LOCUS       DAVKHZ010000001        69036 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000001 DAVKHZ010000000
VERSION     DAVKHZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 69036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.001"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            16..86
                     /locus_tag="VWW99_000001"
     tRNA            16..86
                     /locus_tag="VWW99_000001"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Gly,seq:tcc)
     gene            122..195
                     /locus_tag="VWW99_000002"
     tRNA            122..195
                     /locus_tag="VWW99_000002"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Ile,seq:gat)
     gene            202..289
                     /locus_tag="VWW99_000003"
     tRNA            202..289
                     /locus_tag="VWW99_000003"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Ser,seq:gct)
     gene            complement(415..1350)
                     /locus_tag="VWW99_000004"
     CDS             complement(415..1350)
                     /locus_tag="VWW99_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9537919.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1347..2207)
                     /locus_tag="VWW99_000005"
     CDS             complement(1347..2207)
                     /locus_tag="VWW99_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9537920.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2315..4477
                     /locus_tag="VWW99_000006"
     CDS             2315..4477
                     /locus_tag="VWW99_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9537921.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIYKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLEHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDIVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            4512..5771
                     /locus_tag="VWW99_000007"
     CDS             4512..5771
                     /locus_tag="VWW99_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9537922.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTGRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5785..6651
                     /locus_tag="VWW99_000008"
     CDS             5785..6651
                     /locus_tag="VWW99_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9537923.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6665..7498
                     /locus_tag="VWW99_000009"
     CDS             6665..7498
                     /locus_tag="VWW99_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9537924.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7499..7579
                     /locus_tag="VWW99_000010"
                     /pseudo
     CDS             7499..7579
                     /locus_tag="VWW99_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7856..9298
                     /gene="gtfA"
                     /locus_tag="VWW99_000011"
     CDS             7856..9298
                     /gene="gtfA"
                     /locus_tag="VWW99_000011"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9537925.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTIEIETPTVHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9436..10629
                     /locus_tag="VWW99_000012"
     CDS             9436..10629
                     /locus_tag="VWW99_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9537926.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11316..13295
                     /locus_tag="VWW99_000013"
     CDS             11316..13295
                     /locus_tag="VWW99_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9537927.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13362..14858
                     /locus_tag="VWW99_000014"
     CDS             13362..14858
                     /locus_tag="VWW99_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9537928.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPAAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14858..15784
                     /locus_tag="VWW99_000015"
     CDS             14858..15784
                     /locus_tag="VWW99_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9537929.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15793..16860
                     /locus_tag="VWW99_000016"
     CDS             15793..16860
                     /locus_tag="VWW99_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9537930.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16871..17797
                     /locus_tag="VWW99_000017"
     CDS             16871..17797
                     /locus_tag="VWW99_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9537931.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQSIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            18288..18803
                     /locus_tag="VWW99_000018"
     CDS             18288..18803
                     /locus_tag="VWW99_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9537932.1"
                     /translation="MGILLTSLISLHQLKADKKRDVFRIGISQFITHQSLDATREGFV
                     DELAKQGYVEGENIEIDLQNAQGEQRNLKTISQQLAESSDVVLAIARPSAQSLANTTQ
                     TTPVIFSAVTDPVSAKLVESREHPGGNVTGTSDQSSDAISTQINLIKKVLPKAKTIGI
                     LYTQSEPNSVV"
     gene            18825..19226
                     /locus_tag="VWW99_000019"
     CDS             18825..19226
                     /locus_tag="VWW99_000019"
                     /inference="COORDINATES: protein motif:HMM:NF016292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9537933.1"
                     /translation="MKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISST
                     METVKQVSIKHKVPVFGGSTEMIAVGDLYNYGTNYEELGRQTARMLIRVLKGEKPENI
                     AVELPEKLELHTNQEMADALGIDISKLEGKE"
     gene            19238..20115
                     /locus_tag="VWW99_000020"
                     /pseudo
     CDS             19238..20115
                     /locus_tag="VWW99_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20128..20931
                     /locus_tag="VWW99_000021"
     CDS             20128..20931
                     /locus_tag="VWW99_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9537934.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFIYVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQYPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(20964..21674)
                     /gene="treR"
                     /locus_tag="VWW99_000022"
     CDS             complement(20964..21674)
                     /gene="treR"
                     /locus_tag="VWW99_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9537935.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21859..23826
                     /gene="treP"
                     /locus_tag="VWW99_000023"
     CDS             21859..23826
                     /gene="treP"
                     /locus_tag="VWW99_000023"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9537936.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            24004..25629
                     /gene="treC"
                     /locus_tag="VWW99_000024"
     CDS             24004..25629
                     /gene="treC"
                     /locus_tag="VWW99_000024"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9537937.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25885..26133
                     /locus_tag="VWW99_000025"
     CDS             25885..26133
                     /locus_tag="VWW99_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9537938.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26340..27134
                     /gene="racE"
                     /locus_tag="VWW99_000026"
     CDS             26340..27134
                     /gene="racE"
                     /locus_tag="VWW99_000026"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9537939.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            27131..28102
                     /locus_tag="VWW99_000027"
     CDS             27131..28102
                     /locus_tag="VWW99_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9537940.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            28078..28599
                     /locus_tag="VWW99_000028"
     CDS             28078..28599
                     /locus_tag="VWW99_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9537941.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28596..29057
                     /locus_tag="VWW99_000029"
     CDS             28596..29057
                     /locus_tag="VWW99_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9537942.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            29048..29788
                     /gene="xerD"
                     /locus_tag="VWW99_000030"
     CDS             29048..29788
                     /gene="xerD"
                     /locus_tag="VWW99_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9537943.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29788..30516
                     /locus_tag="VWW99_000031"
     CDS             29788..30516
                     /locus_tag="VWW99_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9537944.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30535..31104
                     /locus_tag="VWW99_000032"
     CDS             30535..31104
                     /locus_tag="VWW99_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9537945.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            31091..31813
                     /locus_tag="VWW99_000033"
     CDS             31091..31813
                     /locus_tag="VWW99_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9537946.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31813..32055
                     /gene="yidD"
                     /locus_tag="VWW99_000034"
     CDS             31813..32055
                     /gene="yidD"
                     /locus_tag="VWW99_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9537947.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(32280..33245)
                     /locus_tag="VWW99_000035"
     CDS             complement(32280..33245)
                     /locus_tag="VWW99_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9537948.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEIATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33341..34093)
                     /locus_tag="VWW99_000036"
     CDS             complement(33341..34093)
                     /locus_tag="VWW99_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9537949.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(34090..35046)
                     /locus_tag="VWW99_000037"
     CDS             complement(34090..35046)
                     /locus_tag="VWW99_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9537950.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(35036..35995)
                     /locus_tag="VWW99_000038"
     CDS             complement(35036..35995)
                     /locus_tag="VWW99_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9537951.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36418..37119)
                     /gene="galE"
                     /locus_tag="VWW99_000039"
     CDS             complement(36418..37119)
                     /gene="galE"
                     /locus_tag="VWW99_000039"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9537952.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHYAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPATEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37520..38584
                     /gene="pepA"
                     /locus_tag="VWW99_000040"
     CDS             37520..38584
                     /gene="pepA"
                     /locus_tag="VWW99_000040"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9537953.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38606..38902
                     /locus_tag="VWW99_000041"
     CDS             38606..38902
                     /locus_tag="VWW99_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9537954.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            39584..40027
                     /locus_tag="VWW99_000042"
     CDS             39584..40027
                     /locus_tag="VWW99_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9537955.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            40031..40795
                     /locus_tag="VWW99_000043"
     CDS             40031..40795
                     /locus_tag="VWW99_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9537956.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            40810..41538
                     /locus_tag="VWW99_000044"
     CDS             40810..41538
                     /locus_tag="VWW99_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9537957.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            41531..43051
                     /locus_tag="VWW99_000045"
     CDS             41531..43051
                     /locus_tag="VWW99_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9537958.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENVIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            43396..44202
                     /locus_tag="VWW99_000046"
     CDS             43396..44202
                     /locus_tag="VWW99_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9537959.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(44369..44905)
                     /locus_tag="VWW99_000047"
     CDS             complement(44369..44905)
                     /locus_tag="VWW99_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9537960.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            45038..45928
                     /locus_tag="VWW99_000048"
     CDS             45038..45928
                     /locus_tag="VWW99_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9537961.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            46172..46525
                     /locus_tag="VWW99_000049"
     CDS             46172..46525
                     /locus_tag="VWW99_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9537962.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46526..47563
                     /locus_tag="VWW99_000050"
     CDS             46526..47563
                     /locus_tag="VWW99_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9537963.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(47610..48617)
                     /gene="galR"
                     /locus_tag="VWW99_000051"
     CDS             complement(47610..48617)
                     /gene="galR"
                     /locus_tag="VWW99_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9537964.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            48782..49960
                     /locus_tag="VWW99_000052"
     CDS             48782..49960
                     /locus_tag="VWW99_000052"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9537965.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49979..51460
                     /locus_tag="VWW99_000053"
     CDS             49979..51460
                     /locus_tag="VWW99_000053"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9537966.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEIMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFAHVLEDAGVYKQTEQGQTAFMRFVEHVGISLD"
     gene            51530..51934
                     /locus_tag="VWW99_000054"
     CDS             51530..51934
                     /locus_tag="VWW99_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9537967.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            52091..52945
                     /locus_tag="VWW99_000055"
     CDS             52091..52945
                     /locus_tag="VWW99_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU9537968.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            52971..53741
                     /locus_tag="VWW99_000056"
     CDS             52971..53741
                     /locus_tag="VWW99_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9537969.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            53728..54594
                     /locus_tag="VWW99_000057"
     CDS             53728..54594
                     /locus_tag="VWW99_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEU9537970.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(54681..55943)
                     /locus_tag="VWW99_000058"
     CDS             complement(54681..55943)
                     /locus_tag="VWW99_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9537971.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(55943..56524)
                     /locus_tag="VWW99_000059"
     CDS             complement(55943..56524)
                     /locus_tag="VWW99_000059"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9537972.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(56924..57019)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(57402..57641)
                     /locus_tag="VWW99_000060"
     CDS             complement(57402..57641)
                     /locus_tag="VWW99_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9537973.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(57641..57784)
                     /locus_tag="VWW99_000061"
     CDS             complement(57641..57784)
                     /locus_tag="VWW99_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9537974.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            complement(57796..58623)
                     /gene="xth"
                     /locus_tag="VWW99_000062"
     CDS             complement(57796..58623)
                     /gene="xth"
                     /locus_tag="VWW99_000062"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9537975.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTITFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            58757..59212
                     /locus_tag="VWW99_000063"
                     /pseudo
     CDS             58757..59212
                     /locus_tag="VWW99_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            59365..59661
                     /locus_tag="VWW99_000064"
     CDS             59365..59661
                     /locus_tag="VWW99_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9537976.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            59867..61612
                     /locus_tag="VWW99_000065"
     CDS             59867..61612
                     /locus_tag="VWW99_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9537977.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQTAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            61602..63353
                     /locus_tag="VWW99_000066"
     CDS             61602..63353
                     /locus_tag="VWW99_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9537978.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEDLLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            63526..64218
                     /locus_tag="VWW99_000067"
     CDS             63526..64218
                     /locus_tag="VWW99_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9537979.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            64261..65487
                     /locus_tag="VWW99_000068"
     CDS             64261..65487
                     /locus_tag="VWW99_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9537980.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFVGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            66107..66337
                     /locus_tag="VWW99_000069"
     CDS             66107..66337
                     /locus_tag="VWW99_000069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9537981.1"
                     /translation="MSDIQVNIPGECLYDKVFVLSFIIYNISTNLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIVK"
     gene            66617..68776
                     /gene="pfbA"
                     /locus_tag="VWW99_000070"
     CDS             66617..68776
                     /gene="pfbA"
                     /locus_tag="VWW99_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9537982.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            68760..>69036
                     /locus_tag="VWW99_000071"
     CDS             68760..>69036
                     /locus_tag="VWW99_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9537983.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELT"
BASE COUNT        20295 a        12597 c        15418 g        20726 t
ORIGIN      
        1 attgatagta tttatgcggg tgtagtttag tggtaaaact acagccttcc aagctgttgt
       61 cgcgagttcg attctcgtca cccgctttga actttgttca aattaccaag ttttttaact
      121 tgggcgcgta gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc ggtggttcga
      181 gtccactcgt gcccattaat tggagaatta ctcaagaggc tgaagaggac ggtttgctaa
      241 atcgttaggt cgggtaactg gcgcaagggt tcgaatccct tattctccgt tttaatgaga
      301 gtttgtaact ctctttttta tttttagagc caataaaact tcagatccgt aaaacttaat
      361 tgaattacta atctgaagtt atttttatat cgtttatatt ataattgtgt tattttactt
      421 ccaactattg agagaaattt cgccactatt tagccagatt tcttttccgt tatcacattg
      481 aactaaaagt tttccatttt ctgagatgtc tttagcaagt cccttgtagt ctttttgctc
      541 tagtgtgaaa gtgacttctt ttcctagaat gaatgactgt tttttgtata ggtataatag
      601 ctcttctgct ggtgtttcga agaaagcacg ccagatttct atgatcaatt cattccttgt
      661 tataggagct gtagctttaa ataagctggc agctttttct tttaattcct gagggaagtc
      721 tttaatagtg aaattgatac ctactccaat aatgatatct gtgactaagc cagtttctac
      781 agaggtcatt gcttcagtaa ggattcctcc aattttatga ttgtttagat agatatcatt
      841 gacccatttt atgtcgacat ctattaaagt taggttctta atggctttgt agacagctcc
      901 agctacaagt agtgtgtagg atggtaattt gtcatagggg agatttggtt taagatggag
      961 tgtcatataa ataccacctt gtggtgagta gaaggaacgt tgaaaacggc ctcggcctgc
     1021 tgtttgatag gaagctagat agagggtatt tgcttcatgg cctaaatcaa ttgcttcttt
     1081 tgcatctagt tgtgttgatt ttgtttcggg tttaaagctg actttaattg gaagattttc
     1141 ttctagaatc tctggaagaa taaggtcacc attcatcagt ttatatcctc tatttttgat
     1201 actatcaatt tcaatgcctt cttgttctag tcgcttgatg gctttccaaa ttgctgttcg
     1261 gcttagggat agtttttctg cgattttttc tccgctgata tagtcggttt ctttagatag
     1321 gatttggtag acagcttggt aggatttcat aatgttgcct ttcttactag ttggtattga
     1381 gagtattctt ttcttgtatg acttggagtc tgattaaagt attgtttata agctttcgaa
     1441 aaatggagtg gatctgaaaa acctaccgag tatgcaatta ccttgatgga ctcttgggta
     1501 ttttcgagaa gttgtctagc tcggtgcatt cgaacgtaga gtaggtattc tttgggtgat
     1561 aaggtattaa attctttgaa tacgcttgat aagtagcttc tgtgaacgga tagttctttt
     1621 gctaaatctt gaattgtaag tgattgagga tagtggctat caattaatcg tttgcattca
     1681 agatagagtt ggtgggttga tgaaatattc ttttttttct gattgggagc aatagttccc
     1741 agatgaaaca tcagttcatg aagttgtccc atgatatgga gttgagctaa ttcacttgat
     1801 tttgtaatct gagcgaagcg gacaatgtct gagatgagtt ttgcagtagt ctgggtatga
     1861 caagtttcag attggatgag ataggattga tcagaaattt gggaaagagc aaaataatca
     1921 ggggctttcc ctccagtgat tcctaaccag tagtaggccc aaggttcttt actatctgct
     1981 tgataaaagg ttagttcctc tggttttaat agaaagaaat ctccttcttt taaatcaaca
     2041 attttaccct tgtaatgaaa ttttccttgt cctttagtaa tgtaatgtag aacgtatgta
     2101 tcacgaatgg ctggaccaaa agagtaatta ggtgtgcatt cctcatatcc ataaaagctt
     2161 agggcaaggt cgattgttcc agtctggtat tctgaaaaaa ctaacatgtg ctacctccta
     2221 cctaacattt taccatattc ttgagacatt tttctatttt ggaaagcgat ttcaggtgat
     2281 aaaatataat caataaagtg atgaggtgaa gtaaatggga gttaggatag agaataatct
     2341 attttatgtt gagagtaaaa atctaagttt gattattgaa aatcgaaatg gctacttact
     2401 tttgaaacat ttaggaaaga ctattaagaa ctataaaggt tccaatagtg tttatgaacg
     2461 agaccatgcc ttttcaggaa atccaacggc tactaatcga acctttagtt tagatactca
     2521 acgtcaaatt tttggacaac atggcttagg agattttagg aaaccaacca tacaggttca
     2581 gcataatgta actgaagtaa cagactttcg atttgtagaa gcaaagattt taaaaggtca
     2641 gaatggtcca cagggcttac cttctccaca tagcatggac gatacagaga ctcttgtctt
     2701 aatgttagaa gattctaagg ctcaacttag tctgactttg tattatacta cttttaataa
     2761 tgatgcgact attgctagct acagtaaatt agataataat agtaatcagg aagttgtcat
     2821 ctataaagat ttttctttta tggctgattt tccagctgca gattacgaaa tagtaactct
     2881 gcagggtgct tatgctcgtg aaaagactgt tagacgtcaa caggtagaac aaggaatctt
     2941 ttcgattagt tcaaaccgag gtgcttctgg tcatgctcaa acaccagctc ttctactatg
     3001 cgaacaagga gtcacagagg atgctgggaa tgtgtttgct attcaactaa tgtatagtgg
     3061 caactttgaa gcttttgttc aaaaaaatca attgaatgaa gttcgggtgg ctattggcat
     3121 taatccagaa aacttttctt ggaagttagc ccctgaggaa tactttgaaa caccggtagc
     3181 tttagtgact cattcagatc agggattaac tggtattagt catgaaagtc agaattttgt
     3241 actggagcac attatgctaa gtgaattttc taaaaaagaa cgtccaattc taatcaataa
     3301 ctgggaagct acttactttg actttcagag agaaaaactg ttagagttag cagatgaagc
     3361 taagaaagtt ggcattgaac tttttgtatt agatgatggt tggtttggca atcgttttga
     3421 tgataatcgt gctttaggtg attgggttgt taatgaggaa aaactgggtg gaagtctaga
     3481 aagtctgatt tcagctatcc atgaaagagg tttgcagttt ggactttggt tagaacccga
     3541 aatgatttct gtagatagtg atttgtatcg tcaacatcct gactgggcta ttcaggttcc
     3601 tggctatgag catacttatt ctcggaatca attagtactt aatcttgcca atcctcaggt
     3661 agtagaatac ttgaaaagtg tcttagatca actcctatct tatcatgata ttgattacat
     3721 taaatgggat atgaaccgca atatcactaa gctagggaat ggattaactt atctagagac
     3781 acagatgcaa tctcatcagt acatgctggg gctttacgaa ctcgtttctt atctgacaga
     3841 gaagcacagc catattctct ttgagtcctg ctctggtggt ggtggacgaa atgatcttgg
     3901 tatgatgcgc tatttcccac aagtctgggc tagtgataat actgatgcca ttgcacgttt
     3961 accaattcaa tacggttcat cctatctcta tccaaccatt tctatggggg ctcatgtgtc
     4021 agcagtaccg aatcatcaga tgggacgaat gacaccatta gaaacacgtg gccttgtagc
     4081 aatgatggga aatttgggct atgaacttga tttgacaaat ttatcagatg aagagaaagc
     4141 tacgattgct aatcaggtga acttgtataa agaattacga ccagtagttc agttaggaca
     4201 gcagtataga ctaattaatc ctgatattgt atccaatgaa gcagctgtac aatttaatta
     4261 cggaaatcaa acgattgtaa cctacgttcg cgttttatct gttgtagaga ccatggaaac
     4321 aactttaaaa ttaaaagatt tggatgaaga gggactatat aaattacagg aaaatggcga
     4381 agtttactca ggtgcagaac taatgtatgc gggcttaact gttatcttat cccaaggaga
     4441 ttttttgagt agacagtata tttttagaag actatgataa gggtgtataa atttataaag
     4501 gaggctttct aatggaatgg tataaaaaaa tcggacttct tgcaactaca ggtttagctt
     4561 tggttgggct cggcgcttgc tccaactatg gtaaatctgc ggatggcaca gtgaccatcg
     4621 agtatttcaa ccagaaaaaa gaaatgacca aaaccttgga agaaatcact cgtgattttg
     4681 agaaggaaaa ccctaagatc aaggttaaag tcgtcaatgt accaaatgct ggtgaagtat
     4741 tgaagacacg cgttctcgca ggagatgtgc ctgatgtggt caatatttac ccacagtcca
     4801 tcgaactgca agaatgggca aaagcaggtg tctttgaaga tttgagcaac aaagactacc
     4861 tgaaacgcgt gaaaaatggc tacgctgaaa aatatgctgt aaacgaaaaa gtttacaacg
     4921 ttccttttac agctaatgct tatggaattt actacaacaa agataaattc gaagaactgg
     4981 gcttgaaggt tcctgaaacc tgggatgaat ttgaacagtt agtcaaagat atcgttgcta
     5041 aaggacaaac accatttgga attgcaggtg cagatgcttg gacactcaat ggttacaatc
     5101 aattagcctt tgcgacagca acaggtggag gaaaagaagc aaatcaatac cttcgttatt
     5161 ctcaaccaaa tgccattaaa ttgtcggatc cgattatgaa agatgatatc aaggtcatgg
     5221 acatccttcg catcaatggc tctaagcaaa agaactggga aggtgctggc tataccgatg
     5281 ttatcggagc cttcgcacgt ggggatgtcc tcatgacacc aaatgggtct tgggcgatca
     5341 cagcgattaa tgaacaaaaa ccgaacttta agattgggac cttcatgatt ccaggaaaag
     5401 aaaaaggaca aagcttaacc gttggtgcgg gagacttggc atggtctatc tcagccacca
     5461 ccaaacatcc aaaagaagcc aatgcctttg tggaatatat gacccgtcca gaagtcatgc
     5521 aaaaatacta cgatgtggac ggatctccaa cagcgatcga aggggtcaaa caagcaggag
     5581 aagattcacc gcttgctggt atgaccgaat atgcctttac gggtcgtcac ttggtctggt
     5641 tgcaacaata ctggaccagt gaagcagact tccatacctt gaccatgaac tatgtcttga
     5701 ccggtgataa acaaggcatg gtcaatgatt tgaatgcctt ctttaacccg atgaaagcgg
     5761 atgtggatta ggagtagaga gactatgaaa aaagtattac aaaaatattg ggcatgggct
     5821 tttgtggtca tccccctctt gttacaagca attttcttct atgtgccgat gtttcaagga
     5881 gccttttaca gttttaccaa ctggacagga ttgacttaca actacaaatt tgtcggctta
     5941 aacaacttta agctcctctt catggatcca aaattcatga atgcgattgg ctttaccgca
     6001 atcattacga ttgccatggt ggttggtgag attgcactcg ggatcttcat tgcgcgtgtc
     6061 ttgaactcta aaatcaaagg ccaaaccttc ttccgtgctt ggttcttctt cccagctgtt
     6121 ttatctggtt tgacagtggc tttgatcttc aagcaagtct tcaactacgg tcttccagcg
     6181 attgggaatg cccttcatat tgaatttctc caaaccagtc ttttagggac taagtgggga
     6241 gcaatctttg cggctgtctt tgtccttctt tggcaagggg tggctatgcc catcatcatc
     6301 ttcctagctg gtttgcaatc tattccaact gagattacag aggcagcaag gattgatggt
     6361 gcgactagca agcaagtttt ctggaacatt gaattgcctt acttgctacc aagtgtctct
     6421 atagtcttta tcctagccct aaaaggtggg ctgactgcct ttgaccaagt ctttgccatg
     6481 accggtggtg gtccaaacaa tgccacaacc tcacttgggc ttttggttta taactatgcc
     6541 tttaaaaaca accaattcgg ttatgccaat gccattgccg taatcttgtt cttcttaatt
     6601 gtagtgattt cgatcatcca attgagagta tctaagaaat ttgaaattta agaggagaag
     6661 catgatgaaa caagatgaaa gaaaagccct gattggcaaa tacattctat tgattctagg
     6721 atcggttctg attttagtgc cgctccttgc taccctcttt agttccttca aatccactaa
     6781 ggatattgta gataatttct ttggctttcc aaccaacttc acatgggaca actttagccg
     6841 tctcttagct gatgggattg gaggctatta ttggaactct gtcgtcatca ctgtcttgtc
     6901 tttacttgca gtaatgatct ttatccctat ggcagcctac tccatcgctc gcaatatgag
     6961 taaaagaaaa gcctttacca tcatgtatac cctcttaatc ctcggaatct tcgtaccttt
     7021 ccaagtcatc atgattccga ttacggttat gatgagtaaa ctcggtttgg ctaatacctt
     7081 tggtttgatc ttgctctact tgacctatgc gattccacag accctctttc tctatgttgg
     7141 ctatatcaaa atctcgattc cagaaagtct ggatgaagca gcagagatcg atggggctaa
     7201 tcaatttaca acctatttcc gcatcatctt cccaatgatg aaaccgatgc atgcgacaac
     7261 catgatcatc aatgcccttt ggttctggaa tgacttcatg ttgccactcc ttgtcttgaa
     7321 ccgggattcc aaaatgtgga ctctgccttt gttccaatac aactacgcag gccaatattt
     7381 caacgactac ggaccaagct ttgcctctta cgtggtcggc attatcagta tcaccattgt
     7441 ctatctcttc ttccaacgcc atatcatttc aggaatgagc aacggggcag tgaagtaata
     7501 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggttgctcaa aacactgttt
     7561 tgaggttgtg gatgaaactg acgaagtcag taaccatacc tacggtaagg tgaagctgac
     7621 gtggtttgaa gagattttcg aatagtataa gaagagctca tggaatctat agtctttgac
     7681 acagaggaag agttcagctt gttgataatt ctatgtagaa aacaagaaag tagtactttg
     7741 tttatcacag tctatattag tacgagtttg atgataaata gagatttaaa atgaatttta
     7801 tttatgaagg cagatcatat gagacttggc ttcaattaga aaaaggagag taatgatgcc
     7861 aattcaaaat aaaaccatgt tgattaccta ttctgatagc cttggaaata atcttaaaga
     7921 cttatatgat aatttggaag agcattttgg agatgctatt ggaggagttc accttttacc
     7981 atttttccca tcaacaggtg atcgtggatt tgcgccagtt gactacgacg aagtggactc
     8041 agcttttggt gattgggagg atgttaagcg tttaggtgag agatattatc ttatgtttga
     8101 ttttatgatt aatcatattt ctcgtcaatc caagtattat aaggactatc aagaaaaaca
     8161 tgaagccagt gaatttaaag atctcttttt aaactgggat aagttttggc cagaaaaccg
     8221 tccgacacag tctgatgtag atttaattta caagcgtaag gatcgtgcac caaagcaaga
     8281 gattgtgttt gaagatggtt cagtggaaca tttgtggaat acctttggtg aggagcagat
     8341 tgatcttgat gtgaccaaag aagtaactat ggaatttatc cgtaagacca ttcagcactt
     8401 ggcaagtaat gggtgtgatt tgattcgtct agatgccttt gcttatgcag tgaagaaatt
     8461 ggatactaat gatttctttg tggaaccaga tatttgggat ttattggaca aagttcgaga
     8521 tatcgctgct gagtatggga cagagctttt acctgagatt catgaacact attcgattca
     8581 gtttaaaata gcagaccatg attactatgt ttatgatttt gctcttccaa tggtgacact
     8641 ttatactctt tacagttcca gaacagagcg tttggctaag tggttaaaga tgagcccgat
     8701 gaagcaattt acgacgctag atacccatga tgggattgga gtagtagatg tcaaggatat
     8761 cctgaccgat gaggagattg actatgcttc aaatgaactc tataaggttg gagccaatgt
     8821 caaacgtaag tactctagtg ccgagtataa caacttagat atctaccaaa tcaattcaac
     8881 ctactattca gcgcttggag atgatgatgt caagtatttt ctcgctcgtc taattcaagc
     8941 ttttgcccca ggtattcctc aggtttacta tgtgggtcta ttagcaggca agaatgactt
     9001 gaaattatta gaagaaacta aagaaggtcg aaatattaat cgtcattact atagcaacga
     9061 ggaaatagca aaagaagtgc aacgatctgt tgtgaaggcc cttctcaatc tattttcttt
     9121 ccgtaaccgt tcagaagcct ttgatctaga agggactatt gagatagaga caccaacagt
     9181 tcacagcatt gtaatcaaac gtcaaaataa agataagtcc gtaacagcag tagcagaaat
     9241 tgatttgcaa aatcagactt atcgggtaat tgagaatgga gttgaagtaa cattttgaag
     9301 ccttgatatg gtggataaaa tagggttttt attttggaaa acgtttcctt tgttttcaaa
     9361 ttgctaaaaa agtggtacaa tataggatag cttactatta tctgaatcag ctgatttgga
     9421 gagaaaggat tcattttgaa atcaataggc tttattgaaa agctgaaggg gttgtctagt
     9481 aaagagctga ttttattggg aattatcctg agtatctttt taccctttta tctttttgta
     9541 gttgtactct gtttatatat tatcagtttg atttttacag gagacatgaa aagtattctt
     9601 cagaaaatgg gggagcatcc gatgctgctt ctttttctta gctatagtac tgttatatcc
     9661 attcttgcac aaaattggat gggtcttgtg gcttcagtag gaatgtttct atttactatt
     9721 ttctttttgc actatcagtc gattttatcc cataaattct ttcgattgat tttgcagttc
     9781 gtcttgtttg gtagtgtctt gtcagctgct tttgccagtt tagaacattt ccaaattgtg
     9841 aagaaattta actatgcttt tctttcaccc aatatgcagg tgtggcatca gaaccgggca
     9901 gaagtgacct tctttaatcc taattattat ggaattattt gttgtttctg tattatgatt
     9961 gctttctatc tgtttacaac gaccaagttg aattggttga aagtattctg tgtgattgca
    10021 ggctttgtta atctctttgg tttgaacttt actcaaaatc gaactgcctt tcctgctatt
    10081 atcgctggag caattatcta tctctttacg actattaaaa actggaaggc tttttggctt
    10141 agtattgggg tcttcgcgat tggtttgagt ttcctctttt ctagtgattt gggagttcga
    10201 atgggtactt tagactcttc tatggaagaa cgcatttcta tctgggatgc tgggatggcc
    10261 ttgtttaagc aaaatccttt ttggggtgaa gggccattga cctatatgca ctcttatcct
    10321 cggatacatg ctccttatca tgaacatgcc cacagtcttt atattgatac gattctgagt
    10381 tacggaattg tgggtaccat tttattagtt ctgtcttctg ttgctcctgt tcgtttgatg
    10441 atggatatga gtcaggagtc ggggaaacgt ccgattatcg gcctttatct atctttcctt
    10501 acagtggttg ctgtgcacgg aatttttgac ttggctctct tctggattca gtcaggcttt
    10561 attttcttgc tagttatgtg cagtattcca ttggagcatc gaatgttggt atcggacatg
    10621 acggattaag ttttataagt ttgaaatctt ctaccttggg taggagattt ttttatacga
    10681 tgggcatgtc gtgcatctga ggtgtaagtc ctctgtaggg atccactacc ggtgaataca
    10741 atagcgctcc ccaagcctga ctatcgtgag gtagcggatt aaaatggtct ggggatagat
    10801 cgttttaagt ctgacgcagg aaataagaat tgtcagagaa agcaatagtg aaatcgtagg
    10861 ctatgaatag aaacgtgata gcgaggagca tatagtggac aaggtagcga gaaattttaa
    10921 agtataaaag atagtcttaa tctatatgtg taaatcacga aagtaattga attcggaata
    10981 aggttggtgt gaaaagacat agtaaactga atcttgaata ctagaatgat acttctacaa
    11041 tattgttata atttaatttg aattccaaaa tcacattgtt ccgtccgtat ttcattttac
    11101 tgtatgtctt cctaagctcc aaaatttctg tgtcaaattt cctattttca ctgtagtctt
    11161 tgacacttcg aacgacaatt tgaatcatat attcaaaaaa aaatgaaaaa tttgtgccaa
    11221 caagcctaaa tattctgaaa atttgtttga tttttaatgg ataatgtgat ataatggttg
    11281 tcaatctata attcacataa acagaggagt agaaaatgaa aaaaaatcgt gtatttgcta
    11341 cagcaggtct tgttttatta gcagcaggtg tacttgcagc atgcagttct tcaaaatcat
    11401 ctgattcatc agcccctaaa gcttatggct atgtttatac agcagaccca gaaaccttgg
    11461 actacctgat ttcaagtaaa aatagtacaa cagtagtgac ttcaaatggg attgatggtt
    11521 tattcactaa cgataattac ggtaatcttg ctcctgcagt tgcagaggat tgggaagtct
    11581 ctaaggatgg tttgacctac acttataaga ttcgtaaagg ggttaaatgg tttacctctg
    11641 atggagaaga atatgcagag gtgacggcta aagatttcgt gaacggttta aaacacgcag
    11701 cagataaaaa atcagaagct atgtatttag ctgaaaattc ggttaaaggc ttggcagatt
    11761 atctatcagg aacttcaaca gatttttcaa cagttggtgt caaggcggtt gatgattata
    11821 cgttacaata cactttgaac cagcctgaac cgttctggaa ctctaagttg acctattcta
    11881 ttttctggcc tctgaatgaa gaattcgaaa catcaaaagg aagcgatttt gctaaaccaa
    11941 cagatccgac atccttgctt tataatggtc cattcttgtt gaaagggttg actgcaaaat
    12001 cttctgtaga gtttgtaaaa aatgagcaat attgggataa agaaaatgtc cacctagata
    12061 ctatcaatct agcttactat gatggatcag atcaggagtc gctagagcgt aacttcacta
    12121 gtggagctta tagttatgcc cgtctttacc ctaccagctc caactattct aaggttgcag
    12181 aagaatacaa ggacaatatc tattacacac aatcaggctc tgggattgct ggtctgggtg
    12241 tgaatattga tcgccaaagt tacaactata cttctaaaac tacagattca gagaaagtag
    12301 ctactaagaa ggcattgctt aacaaagatt tccgtcaagc cttgaatttt gctcttgatc
    12361 gctcagctta ctcagctcaa atcaatggta aagatggagc agctttagca gttcgtaatt
    12421 tatttgtaaa accagacttt gtttcagctg gtgagaagac ctttggtgat ttagtcgctg
    12481 ctcaacttcc tgcttatggt gatgagtgga aaggtgtgaa tttagctgat gggcaggatg
    12541 gtttattcaa tgctgacaag gccaaggcag agtttgcgaa agctaagaaa gctttagaag
    12601 cagacggcgt tcagtttcct attcatctgg acgttccagt agaccaagca tcaaaaaact
    12661 acatatctcg tattcagtcc tttaaacaat ctgtagaaac agttcttggt gttgaaaatg
    12721 tcgttgttga tattcaacaa atgacaagtg atgaattcct taatattact tactatgctg
    12781 ccaatgcttc atctgaggat tgggatgtat caggaggagt ttcatggggg ccagactatc
    12841 aagacccatc tacttacctg gatattttaa aaacaactag cagtgaaact acaaaaacat
    12901 atttaggatt tgataatcca aatagccctt cagtagttca agttggtttg aaagaatacg
    12961 ataaattagt tgatgaagct gccaaagaga caagcgactt gaatgtccgt tatgaaaaat
    13021 atgcagcggc tcaagcatgg ttgacagata gttcactctt tattcctgct atggcttctt
    13081 ctggtgcagc accagtgctt tcacgaattg ttccatttac tggagcttct gcgcaaacag
    13141 gctctaaggg gtcagatgtt tacttcaaat atttgaaatt acaagataaa gcggtgacta
    13201 aggaagagta tgaaaaagct cgtgaaaaat ggttgaaaga aaaagctgaa tcaaatgaga
    13261 aagctcaaaa agaattggca agtcatgtga agtaaataat ttctaataga actttctctc
    13321 catgaggaga aggttctttt gggattttta aaggaaataa tatgaaaaaa tatattttta
    13381 tgcgtgtttt gcggtcattg gtttcgattt tcttagtaac gactttgacc tacacgatta
    13441 tctatacctt ggttcctcga aaattgattt tcaagcagga tcctaactat aataaaattg
    13501 cgacaacggc tgataaacgt gataactatg aaaatactgt gtttgagcgt atgggctaca
    13561 ttgagtatta cgatactaaa gagttgcaag aaaaggcaag tagcatggat tcttctgtaa
    13621 cagtagaagc aaatgcgacc aataaagcta tttatgaaaa gtacatcaat caattaggtc
    13681 atggttggac tttgggagaa tttactgaaa gtggtcaatt ctatgctact cgtgaaattc
    13741 caatttttga acgtgttttt cacttctatg ctaacttgat tgacattgac catacaaata
    13801 aaatccaaga ccctgaaaat ccagacttga aacgctacct tcgttttgaa aatgatccag
    13861 ctatcggatg gtcattggtc ggttcaggaa ctaaacataa atatctcttg tactttaaca
    13921 gtcagttccc atttgttcat caaaactttg tgaacttgaa tttaggtgac tcttacccaa
    13981 cctatgctaa tacaccagtt cttcaggtta ttactcaagg tcaaggacaa accaaaactg
    14041 cccaagttca gttcccaaca ggtaagaaaa cgtcttctgt aaatatttac tcaagaacct
    14101 acaagtcacc tagtcaggct gactctcgtg aagtagctag ctatgggaaa gatgatcctt
    14161 atacagcgac tgaaagtaat taccaatatc catctatgat tgtcagctct gctattactg
    14221 gtttgattgg tttggttctt gcctatgctc ttgccgtgcc acttggttca gccatggctc
    14281 gtttcaagaa cacttggatt gatagcctct caacaggggc tttgaccttc ttgcttgctc
    14341 ttccaacgat tgccttggtt tacatcgttc gattgattgg atcatctatt gcccttccag
    14401 attcattccc tatcttggga gctggagatt ggcgttctta cgttttacca gcagtcatcc
    14461 ttggtttgtt gggtgctcct ggtacagcca tttggattcg tcgttacatg attgacttgc
    14521 aatctcaaga ctttgttcgt ttcgctcgtg caaaaggttt gtctgaaaaa gaaatttcaa
    14581 acaaacacat ctttaaaaat gccatggttc cgctggtttc aggaattcct gctgccatta
    14641 ttggggttat cggtggtgca acccttactg aaacagtctt cgccttccca ggtatgggta
    14701 aaatgttgat tgactctgta aaagcatcta ataactctat ggtcgttggt cttgtcttca
    14761 tctttacatg tatttctatc ttctcacgtc ttttgggaga tatttggatg actattattg
    14821 acccacgtat taaattgact gagaaaggag gcaaataatg tctacaatcg ataaagaaaa
    14881 atttcagttt gtaaaacgtg acgattttgc ctctgaaact attgatgcgc cagcatattc
    14941 ttactggaaa tcagtgttta aacaatttat gaagaaaaaa tcaactgtag tcatgttggg
    15001 aatcttggta gccatcattt tgataagttt catctaccca atgttttcta agtttgattt
    15061 caatgatgtc agcaaggtaa acgactttag tgttcgttat atcaagccaa atgcggagca
    15121 ttggttcggt actgacagta acggtaaatc gctctttgac ggtgtctggt tcggagctcg
    15181 taactccatc ctcatttctg tgattgcgac agtgattaac ttggttatcg gtgtttttgt
    15241 cggtggtatt tggggtattt caaaatcagt tgaccgtgtc atgatggaag tttacaacgt
    15301 catctcaaac atcccacctc ttttgattgt tattgtcttg acttactcaa tcggagctgg
    15361 attctggaat ctgatttttg ccatgagcgt aacaacatgg attggtattg ccttcatgat
    15421 ccgtgtgcaa atcttgcgct atcgtgactt ggaatacaac ttggcgtcac gtactttggg
    15481 aacaccaacc ttgaagattg ttgccaaaaa tatcatgcct caattggtat ctgttattgt
    15541 gacaaccatg actcaaatgc ttccaagctt tatctcatac gaagccttct tgtctttctt
    15601 cggtcttgga ttaccgatta cagtgccaag tttgggtcgt ttgatttcgg attattcaca
    15661 aaacgtaaca accaatgctt acttgttctg gattccattg acaacccttg tcttggtatc
    15721 cttgtccctt ttcgtagttg gtcaaaactt agcggatgct agtgatccac gtacacatag
    15781 ataggagtag aaatgacaaa agaaaaaaat gtaattttga ctgctcgcga tattgtcgtg
    15841 gaatttgacg ttcgtgacaa agtattgaca gccattcgcg gcgtttccct tgaactagtc
    15901 gaaggagaag tattagcctt ggtaggtgag tcaggatcag gtaaatctgt tttgacaaag
    15961 accttcacag gtatgctcga agaaaatggt cgtattgccc aaggtagtat tgactaccgt
    16021 ggtcaggact tgacagcttt atcttctcac aaggattggg aacaaattcg tggtgctaag
    16081 attgcgacta tcttccagga cccaatgact agtttggacc ccattaaaac aattggtagt
    16141 cagattacag aagttattgt aaaacaccaa ggaaaaacag ctaaagaagc gaaagaattg
    16201 gccattgact acatgaataa ggttggcatt ccagacgcag atagacgttt taatgaatac
    16261 ccattccaat attctggagg aatgcgtcaa cgtatcgtta ttgcgattgc ccttgcctgc
    16321 cgacctgatg tcttgatctg tgatgagcca acaactgcct tggatgtaac tattcaagct
    16381 cagattattg atttgctaaa atctttacaa aacgagtatc atttcacaac aatctttatt
    16441 acccacgacc ttggtgtggt ggcaagtatt gcggataagg tagcggttat gtatgcagga
    16501 gaaatcgttg agtatggaac ggttgaggaa gtcttctatg accctcgcca tccatataca
    16561 tggagtctct tgtctagctt gcctcagctt gctgatgata aaggggatct ttactcaatc
    16621 ccaggaacac ctccgtcact ttatactgac ctgaaagggg atgcttttgc cttgcgttct
    16681 gactacgcaa tgcagattga cttcgaacaa aaagctcctc aattctcagt atcagagaca
    16741 cattgggcta aaacttggct tcttcatgag gatgctccaa aagtagaaaa accagctgtg
    16801 attgcaaatc tccatgataa gatccgtgaa aaaatgggat ttgcccatct ggctgactag
    16861 gaggaaggaa atgtctgaaa aattagtaga aatcaaagat ttagaaattt ccttcggtga
    16921 aggaagtaag aagtttgtcg cggttaaaaa tgctaacttc tttatcaaca agggagaaac
    16981 tttctcgctt gtaggtgagt ccggtagtgg gaaaacaact attggtcgtg ctatcatcgg
    17041 tctaaatgat acaagtaatg gagatatcat ttttgatggt caaaagatta atggtaagaa
    17101 atcgcgtgaa caagctgcgg aattgattcg tcgaatccag atgattttcc aagaccctgc
    17161 cgcaagtttg aatgaacgtg cgactgttga ttatattatt tctgaaggtc tttacaatca
    17221 ccgtttattt aaggatgaag aagaacgtaa agagaaagtt caaagtatta tccgtgaagt
    17281 aggtcttctt gctgagcact tgactcgtta ccctcatgaa ttctcaggcg gtcaacgtca
    17341 acgtatcggt attgcccgtg ccttggtcat gcaaccagac tttgttattg cagatgagcc
    17401 aatttcagcc ttggacgttt ctgtacgtgc ccaagtcttg aacttgctca aaaaattcca
    17461 aaaagagctc ggcttgacct atctcttcat cgcccatgac ttgtcggttg ttcgctttat
    17521 ttcagatcgt atcgcagtta tttacaaggg tgttattgta gaggttgcag aaacagaaga
    17581 attgtttaac aatccaattc acccatatac tcaagccttg ctttcagcgg taccaatccc
    17641 agatccaatc ttggaacgta agaaggtctt gaaggtttac gacccaagtc aacacgacta
    17701 tgagactgat aagccatcta tggtagaaat ccgtccaggt cactatgttt gggcgaacca
    17761 agccgaattg gcacgttatc aaaaaggact aaactaataa tggttttata atttccatgt
    17821 caatttttat gggaattata aaactttttt tgttggactg atttgaaaaa ctttgaaagc
    17881 ttcctgaaag aattttcgaa aaattttaaa aaattttgaa atattattga caggtgatga
    17941 aaaaggtggt aggataggaa ggatcaaaaa agaaagcaga aaaagaaatg aataagagac
    18001 aagccgtaac tatgaatcaa aacttttact ttagctactt tagatagtgt ttgtagacat
    18061 gtcaccatgg atgcaaacag ttcaagcaat ttgtttgtat ctatgtggct aagatttttg
    18121 attgtggtcc atatagatga atatctaaat ggactagcta agttgtaact tgttaggtta
    18181 tttcaagtac ccgtttagat agtatctagg cggttttttg ttttatcttt tctgagaagg
    18241 agtttcatta tgaaagttgt tcgaaaatca ctagccccct cttggtagtg gggatcctct
    18301 tgacttctct gatcagtttg catcagttga aggcagataa gaaaagagat gtctttcgta
    18361 tcggtatttc gcagtttatt acccaccagt ccttagacgc tactagagaa ggatttgtgg
    18421 atgagctggc caaacaaggc tatgttgagg gggaaaatat tgagattgat ttgcaaaatg
    18481 cacagggaga acaaagaaat ctaaaaacga tttcccagca actagcagaa tccagcgatg
    18541 tcgttctagc catcgcaagg ccttctgctc agagcttggc caatacgacg caaacgacac
    18601 cagttatctt ttcagctgta acagacccag tcagtgccaa gttggttgag tcaagagaac
    18661 atcctggggg caatgtaact gggacaagcg atcagtcatc agatgccatt tcaacccaaa
    18721 tcaatttgat aaagaaagtg ttgccaaagg ctaaaacgat tggaatcctc tatactcaga
    18781 gcgagcccaa ttcggttgtt taaaatgatg aagctaagcg acttctgaaa gaaaaaggct
    18841 ttaccgttgt tgaaaaaaca atcttggaca gtaataacgt caaggcggca gcagagagtt
    18901 tgatggcaga ggtggatatg gtttttgtcc caactgataa tatcatttcg tcaaccatgg
    18961 aaacagttaa gcaggtttct attaaacaca aggttccagt attcggtggt tcaacagaaa
    19021 tgattgctgt tggtgacttg tataactacg gaaccaatta tgaagaattg ggaagacaga
    19081 cagctcgcat gctgattcgt gttttaaaag gtgagaaacc agaaaatata gcagttgagt
    19141 tgcctgaaaa actggaattg cataccaatc aagaaatggc agatgcattg ggaattgata
    19201 ttagtaagtt agaaggcaag gaataaggag gattaagatg aattttgtgt tatctagttt
    19261 atcagaaggt ttgttgtggt cgattgtggc gattggggtc tacttgactt tccgtatttt
    19321 ggatattgcg gatataactg ctgagggagc ctttcctctg ggggcagcag tcgttgtatc
    19381 acagatacag gcaggggcaa atccttggct tgcgacttta cttgctttgc tggcaggtat
    19441 ggtagcaggt cttgtatcag gaatgctcca caccaagatg aaaatcccag ctctcttgac
    19501 agggattgtg accttgacag ggctctattc aatcaatatt aaaattatgg gcagtgtacc
    19561 caatctttcc ttgggagatt ctgcaaccgt ctttaaacag ttggcgagct tgggtctgac
    19621 aaatgaagga gctgttttct cacttagttt agtctgtctc ttacttgttt ggtcttgacc
    19681 cttttgatga aaacagagat tggtttggtc ttgcgttcga ctggggataa tatcccgata
    19741 agtgaggcca atggagtcaa tgtagacacc atgaagattg ttggttacat gatttcaaac
    19801 ggtttgattg ccctatgtgg ttccttgttt gcccaaaatg atggattttc agatgtgact
    19861 tctgggacag gaaccattgt tgttggtttg agctcggaag tcttgataca cgacttgacc
    19921 attggaggcc gcttattatc catcggaatc ggtgctattg tttaccgttt gattatttta
    19981 aatatctatg aaattccaaa tctagatcaa aacttggtac gtctctttaa tgcaatcttg
    20041 ctcgctttgg ttttatttgc accagaattg caaaagagat taaagattcg tggtctgaaa
    20101 ttgagaaatg aataggagga gaaagttatg gcaagtttgt taacacttga aaatattcac
    20161 aagacatttg aagcagggac ggtcaatgag aaccatgtcc tcaaaggatt agatttagag
    20221 gttgaagagg gagattttat ctatgtgatt ggtggaaatg gagcaggaaa atccactttg
    20281 atgaatatct tggctggcaa tctatcggtg gacgaagggg accttctact ggcagggaaa
    20341 tccattaaaa atctgagtgt cagaaagaga gccaaggata ttgcccgtgt ttttcaatat
    20401 cctaagatgg gaacagcttc tcgtttgacg attgaggaga atatggctat tgccttgaga
    20461 cgtgggcaaa agagagggct tggttggggt gtgaaggaga aggatagaat ccagttccaa
    20521 gaggccttga aggagttgaa tattggtctt gaaaaccgct taaaagtaga tactcaatat
    20581 ctctcaggag gacaaagaca ggctttgacc ctagtcatgg cagctctggt gaaacccaaa
    20641 cttttacttt tggatgaaca tactgcggca cttgacccga aaaccagtca aatggtgatg
    20701 gacttgacgc aaaagattgt ggagcaccat cagtggacga ctttgatgat tacccatgat
    20761 atgaatcatg cgattgagta cggcaatcgt ctcattatgc tctatcaagg caagatagtg
    20821 gtggatgtca agggagaaga gaaaaagcat ctgacagttg aagaccttat gcatctcttc
    20881 cagaaaaata gtggccaaag tctggtcagt gatgaattag ttttgggata aagaaaaagg
    20941 ctgagatctg gtgtctcagt cttttatttc ttgcgttttg caaaatctac aaatctaaat
    21001 ttttctaact tatggcgact ttcggtaaac tgaaattggc gtccgtcttg gagatagact
    21061 tttgatttaa tcgaaacgac ataagggtct ttgccaatgt ccatgagaat cttgtctcgg
    21121 tcgcttgagt ggtcaatggt gatttccttc tgagcataat caataaggag tttgaggcca
    21181 ttttctatat aagaatagat agactgctca gcaatttggc gagtgagatt tgggatgagt
    21241 tccatatcca gatagtccgt atccagaacg gataccagat catccaccac acgctggcgg
    21301 accaccttcc aaaccatccg aaactctggg aaaccggtta tcagtgagga ttttttatca
    21361 ataataatct tgtccagact gaccacgttg gttttagagc gcagtccaag ttctttaact
    21421 agttcttggt agctggttag gttggataca gggaaattga cggtttcttc tttgacgact
    21481 tgagaacctt gccctcttat ctttttgatc aatccttcct cttggaggag agacagggcc
    21541 tttcggacgg tatcacgact cacttgatat tgctccataa ggtcgtgctc gctaggaagg
    21601 aaatctccga cagcgtaagt ctcgttttga atggtttctt ggatttgctt aaataattgt
    21661 tggtatttct tcattttgcc tgctttaata ggtttttagt ctagtttaat gaacacctgt
    21721 atctatttta ccatacaagt gctagaaaat ttacaaaaaa acaccctttt tataccaaac
    21781 aagttgcaga caagtttggt atcgtttaca atatacttat caaatcaaac ttgctttgac
    21841 aagtataagg agaatcaaat gggaaaattt gaacaagaag ccaaagatct gcttcaggca
    21901 attggaggca aagaaaatgt gactgccgta actcactgtg cgacacggat gcgctttgtt
    21961 ttaggagatg ataagaaggc taatgttaaa gctatcgagt caattccagc tgttaaagga
    22021 acttttacaa atgcaggtca atttcaggta atcattggaa atgacgtgcc catcttttat
    22081 aatgatttta cagccgtttc aggtattgag ggtgtttcca aagaagcagc caagtctgca
    22141 gctaagagta atcaaaacgt ggtccaaggt gttatgacca ctctggcgga gatttttact
    22201 ccgattattc cagccttgat agtcggagga ttgatcctcg gtttccgtaa tgtcttggaa
    22261 ggtgtccatt ggtcgatgtt ggatggcaag accatcacag aatcctctca gttttgggca
    22321 ggtgtcaatc acttcctctg gttgcctggt gaagctatct tccagttctt accagtaggg
    22381 attacttggt ctgtttctcg taagatggga accagccaaa ttttgggaat tgttctcgga
    22441 atctgtttgg tatcgcctca gttgctcaat gcctatgcgg ttgcttcaac gccagcagct
    22501 gatatcgcgg caaactgggt ttggaatttt ggctatttta ctgttaatcg tatcggttac
    22561 caagcccaag ttatcccagc cttgcttgca ggtttgagtc tgtcttatct tgaaatcttc
    22621 tggcgcaagc atatcccaga agtcatttct atgatttttg tacctttctt gtcattgatt
    22681 ccagccttga ttttggctca tactgttttg ggaccaatcg gttggacaat tggacaagga
    22741 ctttcatcag ttgtcttggc aggtttaact ggtccagtta aagggctctt cggtgcaatt
    22801 tttggtgccc tctacgctcc atttgtcatc acaggtctgc accatatgac caatgccatt
    22861 gatacacaat tgattgcgga tgctggtggc actgccctat ggccaatgat tgctctttct
    22921 aatattgctc aaggctcagc cgtgtttgcc tattatttca tgcatcgcca tgatgagcgt
    22981 gaggctcagg tttcacttcc tgcaaccatt tcagcctatc tcggtgttac agaaccagct
    23041 ctttttgggg ttaacgtaaa atatatttat ccatttgttg ctgggatgac tggttcagcc
    23101 cttgcaggca tgttatccgt tacttttaat gtaactgcgg cttctattgg tatcggtggt
    23161 ttgccaggta ttctctctat tcaacctcaa tacatgctgc catttgcagg aactatgcta
    23221 gttgcgattg ttgttccaat gctcttgact ttcttctttc gcaaggctgg tctcttcaca
    23281 aaaatagagg gcgatacgaa cttgcaggca gaattcgttg ctcaagaaga agcagaattt
    23341 gtgaaccatg aaccagtaga acttacttcg gtagaaatta tcagcccact aactggccaa
    23401 gtgaaagaat tgagtcaagc gacggatcct gtttttgcat caggtgtcat ggggcaaggt
    23461 ctggtcattg aaccaagcca aggtgagttg acctctccag ttaatggaac agtgacggtt
    23521 cttttcccta ccaagcatgc catcggcatt gtctctgacg agggagttga attgctcatc
    23581 cacatcggta tggatacagt aggtcttgat ggcaaaggtt ttgaaagtct tgtagtccaa
    23641 ggagatcacg ttatagttgg ccagcaactg attcgttttg atatggatgt cattaaggct
    23701 gcaggtctgg tgacagaaac tcctgttatc atcaccaacc aagatgctta tacagcgact
    23761 attactggaa cttatccgac aacgatccaa gctggagcat ctctcatggt cgctacacga
    23821 atctaatact cttcgaaaat ctcttcaaac cacgtcagct ctatctgcaa cctcaaaata
    23881 gtgttttgag caacctgtgg ctagcttcct agtttgttct ttgattttca ttgagtataa
    23941 ttgaaatagc acaaataggg acggaagact gcttcctccc ttttatcaga aaaggagaaa
    24001 cagatgacac ttgataaagg aaaagtagtc tatcaaatct atccaaaatc ttacaaagac
    24061 accactgaaa acggtattgg ggatttccgt gggattatcg aaaaaattcc ctatttagcc
    24121 aaactaggtg tggatatggt ctggctcaat ccattctatc caagccctca acgggataat
    24181 ggttacgata tttcagatta tatggcagtg gatcctcttt ttggtgatat ggctgatttt
    24241 gaggaaatgg tgtgtatcgg taaagagcac aagattgact ttatgctgga catggtactt
    24301 aatcattgtt cgacagagca tgaatggttt cagaaagccc tagctggtga taagtattat
    24361 caagactttt tcttcatcca agaccaacca acagattggc agtctaagtt tggtggttct
    24421 gcatgggcgc ctttcgggga tactgggaaa tactatcttc acctatttga tgagacccag
    24481 gctgacctta actggcgcaa tcccaatgtc cgtaaggagc ttttcaaggt tgttaatttc
    24541 tggcgcgaca agggtgtcaa aggttttcga tttgatgtga tcaatttgat tggtaaggac
    24601 gaggtttcag tggactgtcc tgaaaatgaa gggaagccag cttatacaga caaacccatc
    24661 gttcataact atttgcgtat gatgaaccaa gccacttttg gatctgacga tagctttatg
    24721 acagttgggg aaatgtcttc taccaccatg gaaaactgtg tcctctattc gtcgcctgat
    24781 cgtcaggaat tatccatgac ctttaatttt catcacctca aggtggacta taaggatgga
    24841 caaaagtgga ccttggctcc ctttgatttt gaagagttga agagtcttta ccatagttgg
    24901 ggtaaggaaa tgagtgataa agacggttgg agtgccctct tttggaacaa tcacgatcaa
    24961 ccacgtgcct tgaaccgttt tgtcgatatt caaaacttcc gcaaggaagg agccaccatg
    25021 ctagcagcca gcattcacct gtcacgtggg acaccttata tctacatggg tgaggaaatc
    25081 gggatgattg acccagacta tgattctatg gctgattatg tggatgtcga atcgcttaat
    25141 gcctatcaga tgctcttaga agaaggaaag agtcagcaag aagccttcca gattattcaa
    25201 gctaagtcgc gtgataattc acgcattccc atgcagtggg atgcttcgga aaatgcagga
    25261 ttttcaacag gcactccttg gttgaaagct ggtaaatcct ataaatacat caatgtagaa
    25321 aatgaaatcc agggtccgat ttttaccttc tatcaagact tgattcgact gcgtaaggaa
    25381 atgccaatca tttcagaagg aagttacaaa ccagcctttg aagatagcaa acaagtctac
    25441 gcttttgaac gccagtttga ggatcaaaag ttactagtgc tcaataactt ttatgctaaa
    25501 gaagtggaaa tcgacttgcc agcagtctac caaaatggac agattttgat atcaaactat
    25561 gaagatgcag aagtgtctga gaaaattcta ctcaaaccct atcaaacact ggctatttat
    25621 gtaaattaat actcttcgaa aatctcttca aaccgtgtca gttttatctg caacctcaaa
    25681 gcggtgcttt gagcaacctg tggctagctt cctagtttgc tctttgattt tcattgagta
    25741 taaacttagc aaggagacct ctttttcaga aaagcagggt tctttttgct gtctattcca
    25801 tccttgaaag tgtagaacaa actggtcagg gtggctagtt tgtggtataa tgaaagggac
    25861 tcaaaaagaa acaggagaaa aatgatggat ttacttttag caattgtatt gattgtgcta
    25921 gcttttctag gaggagctct tggaggaatg tacttggttc gtaagcaaat cgaaaaagaa
    25981 tttgctgaca acccacgttt gaatgctgaa gcagttcgta ctcttttgag tgcaaatggt
    26041 caaaaaccaa gcgaagctaa ggtacaacaa gtttaccacc aaatcatccg ccaacaaaag
    26101 gcagcccttg ctaacaataa aaagaaaaaa taaataagga aaagtctggg atgaaagttc
    26161 cagacttctc actatgttgg ttatggttta gggatgagac tttttccttg cattctattg
    26221 atatttgatt atgattaaga gagaggtagt tgacattcta tcaagctgtc ggtcagttcg
    26281 ttcagtacaa ggaatcataa tgaacgatca atcagaaaaa agactagaaa gaagactgta
    26341 tggataatcg accaattggt tttttggatt cgggtgtcgg gggcttgacc gttgtgcgcg
    26401 agctcatgcg ccagcttccc catgaagaaa tcgtctatat tggagattcg gcgcgggcgc
    26461 cctatggccc ccgtcctgct gagcaaattc gtgaatatac ttggcagctg gtcaactttc
    26521 tcttgaccaa ggatgtcaaa atgattgtca ttgcttgtaa cactgcgact gcggtcgtct
    26581 gggaagaaat caaggctcaa ctagatattc ctgtcttggg tgtaattttg ccaggagctt
    26641 cggcagccat caagtccagt caaggtggga aaatcggagt gattggaacg cccatgacgg
    26701 tacaatcaga catataccgt cagaaaatcc atgatctgga tcccgactta caggtggaga
    26761 gcttggcctg tcccaagttt gctcccttgg ttgagtcagg tgccctgtca accagtgtta
    26821 ccaagaaggt ggtctatgaa accctgcgtc ccttggttgg aaaggtggat agcctgattt
    26881 tgggctgtac tcattatcca ctccttcgcc ctattatcca aaatgtgatg gggccaaagg
    26941 ttcagctcat cgatagtggg gcagagtgcg tacgggatat ctcagtctta ctcaattatt
    27001 ttgaaatcaa tcgtggtcgc gatgctggac cactccatca ccgtttttac acaacagcca
    27061 gtagccaaag ttttgcacaa attggtgaag aatggctgga aaaagagatt catgtggagc
    27121 atgtagaatt atgacaaata aaatttatga atataaggat gaccaggact ggtatgttgg
    27181 gtcttatagt atttttggtg gcgttaacag tttgagcgac tataagacag attttcctct
    27241 gtttgaattc tccaaaatat ttggagatga agagtatggt ttcccgcttt cagttactgt
    27301 tttacgctat ggttctacct accgtttgtt ctcctttgtg gtagatatgc ttaatcaaga
    27361 aatgggacga aacttggaag ttattcaacg tcatggggcc ctgctcttgg ttgaaaatgg
    27421 gcaactcttg tatgtagaat tgcctaaaga aggggtcaat gttcatgatt tctttgagac
    27481 aagcaaggtc agagaaacct tgttgattgc gactcgtaac gaaggcaaaa ccaaggaatt
    27541 ccgagctatc tttgataagt taggctacga tgtggaaaat cttaatgact accctgacct
    27601 gcctgaagta gcagaaacag gtatgacctt tgaagaaaat gcccgcctta aggcagaaac
    27661 catttctcaa ttaacgggta agatggtttt ggcagatgat tctggtctca aagtcgatgt
    27721 ccttggtggc ttaccaggcg tctggtcagc tcgtttcgca ggtgtgggag caactgaccg
    27781 tgaaaataat gccaaactct tgcacgaatt ggccatggtc tttgaactca aggaccgctc
    27841 ggctcagttc cacacaaccc tagtcgtagc cagcccaaat aaggaaagtt tagttgttga
    27901 agcagactgg tcaggttata ttaactttga acctaagggt gaaaatggct ttggctatga
    27961 tcccctcttc cttgtaggag agacaggtga gtcatcagct gaattaaccc tagaagaaaa
    28021 aaatagtcaa tctcaccgtg ccttagccgt taagaaactt ttggaggtat ttccatcatg
    28081 gcaaagcaaa ccatcattgt aatgagcgat tcccatggcg atagcttgat tgtggaagaa
    28141 gtccgtgatc gctatgtggg caaagtcgat gccgtttttc ataacggcga ttctgaacta
    28201 cgtccggatt ctccactttg ggagggcatc cgcgttgtta aagggaacat ggacttctac
    28261 gccggctacc cagaacgtct ggtgactgag cttggttcga ccaagattat ccaaactcat
    28321 ggtcacttgt ttgacatcaa tttcaacttt caaaagttgg actactgggc tcaggaggaa
    28381 gaggccgcta tctgcctcta tggtcacttg catgtgccaa gtgcttggtt ggaaggcaag
    28441 atcctctttc taaatccagg ttctatcagt caaccacgag gtaccatcag agaatgtctc
    28501 tatgctcgtg tggagattga tgatagttac ttcaaagtgg actttttgac acgagatcac
    28561 gaggtgtatc caggtttgtc caaggagttt agccgatgat tgccaaggag tttgagactt
    28621 tcttgttggg gcaggaggaa acttttttga cccctgctaa aaatctagct gtgttgattg
    28681 atacccacaa tgcggatcat gcgaccctct tgctcagtca gatgacctat acccgtgttc
    28741 ccgttgtgac agatgaaaaa cagtttgttg ggacgattgg actcagagat attatggctt
    28801 atcagatgga gcatgacttg agccaagaaa tcatggcgga tacggatatc gttcatatga
    28861 caaaaacgga cgtagcggtt gtttcgcctg atttcaccat tacggaggtc ttgcacaagc
    28921 tagtagatga gtccttctta ccggtcgtgg atgcagaggg tattttccaa gggattatta
    28981 cgcgcaagtc catcctcaag gccgttaatg ccctcttgca tgactttagt aaggaatatg
    29041 agattcgatg ccaatgagag acaggatttc agccttttta gaggaaaagc agggcttgtc
    29101 tgtcaattcc aagcagtcct ataagtatga tttggagcaa tttttagaca tggtaggtga
    29161 gcggatttct gagaccagtc tcaagattta ccaagcccag ctagccaatc taaaaatcag
    29221 cgcccagaag cgaaagattt cggcctgtaa ccaatttcta tactttctct atcaaaaagg
    29281 agaggtggac agcttttacc gcttggaatt agccaaacaa gctgaaaaga agacggaaaa
    29341 gccagagatt ctatacctag actctttttg gcaggaaagc gaccatccag agggccgctt
    29401 gctagcgctc ttaatcctag aaatggggct cttgcccagt gagattttag ccatcaaggt
    29461 tgcggacatc aatctggatt ttcaggtgct gcgaatcagc aaggcttccc aacagaggat
    29521 tgtcaccatt cccacggcct tgctttcaga attggaaccc ttgatggggc agacctatct
    29581 ttttgaaaga ggagagaaac cctattctcg tcagtgggcc tttcgtcagt tagaatcttt
    29641 tgtcaaggag aaaggttttc catccttatc agctcaagtc ttacgtgaac agtttattct
    29701 aagacaaata gaaaacaagg tcgatttgta cgaaattgca aaaaaattag gattaaaaac
    29761 agtcctgacc ttagaaaaat atagataatg gatattaaat taaaagattt tgaaggaccc
    29821 ctggacttgc tcttgcatct ggtttctaag taccagatgg atatctacga tgtgcccatt
    29881 acggaagtca tcgaacagta tctagcctat gtctcaaccc tgcaggccat gcgtctggaa
    29941 gtgacgggtg agtacatggt catggctagt cagctcatgc tgattaagag tcgtaaactc
    30001 cttccgaagg tagcagaagt gacagacttg ggggatgatc tggagcagga cctcctctct
    30061 caaatcgaag aatatcgcaa gttcaagctc ttgggtgagc acttggaagc caagcaccaa
    30121 gaacgggccc agtattattc caaagcgccg acagagttga tttacgaaga tgcggagctt
    30181 gtgcatgaca agacgaccat tgacctcttt ttggcttttt caaatatcct agccaagaaa
    30241 aaagaggagt ttgcacaaaa tcacacgact atcttgcggg atgagtataa gattgaggac
    30301 atgatgatta ttgtgaaaga atccttgatt ggacgagatc aattgcgctt gcaggatttg
    30361 ttcaaggaag cccagaatgt ccaagaggtc atcaccctct ttttggcaac cctagagtta
    30421 atcaaaaccc aggagctgat cctcgtgcaa gaggagagtt ttggagatat ctatctcatg
    30481 gaaaagaagg aagaaagtca agtgcctcaa agctagactt gatagagagg aaagatgagt
    30541 actttagcaa aaatagaagc gctcttgttt gtagcgggtg aagatgggat tcgggtccgc
    30601 cagttagctg aactcctctc tctgccaccg acaggcatcc agcaaagttt aggaaaatta
    30661 gcccagaagt atgaaaagga cccagattcc agtttggctt tgattgagac aagtggtgct
    30721 tatagattgg tgaccaagcc tcaatttgca gagattttga aggaatactc taaggcgcct
    30781 atcaaccaga gcttgtctcg ggctgccctt gagaccttgt ccattattgc ctacaaacag
    30841 ccgattacgc ggatagaaat tgatgccatc cgtggagtta actcgagtgg agccttggca
    30901 aagttgcagg cttttgacct gataaaggaa gacgggaaaa aggaagtatt ggggcgcccc
    30961 aacctctatg tgactacgga ttatttccta gattacatgg ggataaacca tttagaagaa
    31021 ttaccagtga ttgatgagct tgagattcaa gcccaagaaa gccaattatt tggtgaaagg
    31081 atagaagaag atgagaatca ataagtatat tgcccacgca ggtgtggcca gtaggagaaa
    31141 agcagaagag ctgatcaagc aaggtttggt gacggttaac ggccaagtgg tgcgtgaact
    31201 agcaaccact atcaagtcag gcgacaaggt cgaagttgaa ggtcaaccta tctacaacga
    31261 agaaaaggtc tattatctgc ttaacaaacc acgcggtgtc atttccagtg tgacagatga
    31321 taagggtcgc aagacggttg tcgacctctt gcccaatgtc aaagagcgta tttaccctgt
    31381 gggtcgtttg gactgggata catcaggagt cttgattttg accaatgatg gggactttac
    31441 agacgagatg attcaccctc gtaatgagat tgacaaggtt tatgtcgcgc gtgttaaagg
    31501 tgtggccaat aaggacaatc ttcgcccctt gacccgtggt cttgagattg atggtaagaa
    31561 aaccaagcca gctgtttatg aaattctcaa agtggaccca gtcaaaaatc gctctgtggt
    31621 gcagttgacc atccatgaag ggcgtaacca tcaggttaaa aagatgtttg aagctgttgg
    31681 tctccaagta gataagttgt ctcggactcg tttcggacac ctagacttga caggactccg
    31741 tccaggagaa tcccgtcgtc ttaataaaaa agaaatcagc caactacaca ccatggctgt
    31801 aactaagaaa taatgaaacg aattttaata gcgcctgtgc gcttttacca acgttttatc
    31861 tcaccagtct ttccaccctc ttgtcgcttt gatctgactt gttccaacta catgattcag
    31921 gctattgaaa aacatggttt taagggagta ttgatgggct tggctcggat tttacgttgt
    31981 catccctggt cgaaaacagg taaggacccc gttccagacc acttttccct taaacgaaat
    32041 caagaagggg aatgaggtgg ggtaaataga tttcaaaatg ataaaaacgc atcctatcag
    32101 gtttgagtga acttgatagg atgcgtttta gaatgtcaaa attttatact cttagaaaat
    32161 ctcctcaaac cgcatcagct ttcatctgca acctcaaaac agtgttttga gcaacctgcg
    32221 gctagtttcc tagtttgctc tttgattttc attgagtatt aaattgagtt tgaagtggct
    32281 tatttcaaag ctttttgtat gtcttcaatc atgagttttg ttgattcaag tccgcctccg
    32341 cttagatacc agaggtctgg tgttagttgg ataatcttac catttttagc agcaggtgtt
    32401 tcagcgataa gggcattttc taggacaccg tcgttgctag agttgtcccc accgatggca
    32461 agggtacggt tgatgacaaa gaggatgtca gggttgattt ctttgacact ttcaaagctg
    32521 acttcttgtc cgtggcgtga gtcttcaaat gttgtatcag ttggtttgaa tttcaaggtt
    32581 tggtacaaga aagagaaacg agatttggca ccaaaggctg ccatttttcc ttcattaagg
    32641 aggatcgcaa gggctttttt gtcagagctt tcatttttag tagcgatttc ttggatgctc
    32701 ttgtctagct tggccaattc ttccttggct ttctgtgtac cagtttcgcc gaaggcactt
    32761 gctaaggatt cgatattagc cttggtagaa gtccagtagt cgtccttgct tgcttggaag
    32821 agaacggttg gggcgatttc tttgaatttg tctacgaatt tttgtgtacg tggtgaagcg
    32881 ataatcaaat caggctcaag ggcggcgata gcttctaaat caggttcttt catagaacca
    32941 acatttttga cagttcccac taggtctttt agataagtcg gaacagtttt tgtaggcatt
    33001 ccgacgatat ttttttcaaa tcctaaagcg cgaatagtat ccgcagcgcc gaggtcaaag
    33061 gtcacaatct tttcaggaac tttggaaagt ttgacctcgt ccagtgaact tttaatggtt
    33121 acctctgttg gagcagagct actggtctct gtctgactag tgcttgagtt tgtactacat
    33181 gcaccaagta ggagcaagaa gctggccact agggcagtga aataaagttt aagggatgtt
    33241 ttcataattt ctccttttta aaatgtgata acgatttagg gagtctctta atcttattga
    33301 ctaagagact gaaggttctc taacttgagc ttttatgtta ctagctatag atacagatct
    33361 ttttgtcatt gatatcagct agcgtgatgg gaatctcata aagttgactg agcaggtcag
    33421 cctgcatgat ttgatcggtt cttcccttgc taaagacctg gccgtccttg aaggcgacaa
    33481 tttcatctgc atactgactg gccatgttga tatcgtggag gacgatgata atggtcttgc
    33541 cgagttcctc caccagtcgt cgaagaatct gcatcatgct gacgctttgc ttgatatcga
    33601 gattgttgag tggttcgtcc agcaagataa agtccgtatc ctgggccagt accatagcga
    33661 taaagacgcg ctggagttgc ccccctgaca ggctattgat gtagcggtct tttaagttgg
    33721 tcagttccaa atagttcaga gtttctcgga ttttttccca gtcttctgat ctaagtcgac
    33781 ctcggctgta gggaaaacgt ccaaaactga ccagttcttc aacagtcaat ttggcttggt
    33841 aattgatttt ctgttttagg atggttagtt cttgggccag ttcttgcgaa ttccagctct
    33901 cgatttcacg tcctttgata ctgagaactc cctgatcttt cttggttagt ctgctcatga
    33961 tggagaggag agtcgatttt ccagcaccat ttggaccaat aaaggctgtc agtttttgag
    34021 gactgacttc aagcgaaatg ccttgcaaaa tatcctgttt ttgaatggat ttgtcaatgt
    34081 tttccagttt cactgacgag acctcctata tagtaagata aagaataaga agccacccac
    34141 actctcaatg atcatactga tacgaatttc cagtgcaaag actcgttcaa tcaaggcttg
    34201 ccccaaggtt aaactaataa atccaaccag aatggccact ataaagagta acttgtgctg
    34261 atagtctttg acaatcaggt aggtgaggtt ggccagcata aagccgaaga aggccatagg
    34321 tcctaccaag gcagtggccg ttgaggtcaa aagcacgatt ccccagagga gctctttctg
    34381 ttctttttca acatcgagtc ccaatatctg agccgtttct ctttgcaggt gcaagacatc
    34441 tagaacgact gcttttcgaa agaaaaagat tgtcaaagcg aggatgatca gagaaccgat
    34501 ggctaggatg gaagtgttaa gatgttgaaa ggaggcaaaa agactatttt gcagtttatc
    34561 gtattcgttt ggatccatta ggacttgaag gaaggtgctg atatttcgaa agagacttct
    34621 gagcgctaga cagatcagca ggatgaagac caggtcttgc ttcatcagtg tcttcaagta
    34681 accttgtaag gcgagaaaga agagggactg gacaagaagt aagactagga attctaagat
    34741 aggggatttg ccaagttgaa gaaacttgct ttcaaaaacc agtagtaggg tttgtagtag
    34801 gacgtagaag gattcaattc ccaaaatact aggcgtcagg aagcgatttt ccgtcagggt
    34861 ttgaaaacta atggtcgaaa tcccagtcgc gatggctacc aagagataaa cgatgatctt
    34921 ttgggaacgc aacttccaag caaaggctga caagtgagtg atgggccaaa agtagagaag
    34981 acaagctccg atggcaagaa taatgagaat ccagaagagc ttggtatgtt tgcttttagt
    35041 ctgcatcttt tcgtccccct ctccagagaa gtaggataaa gacgagacta ccgatgattc
    35101 ctagcaagag actgacagac aactcatagg gcctaatcag aactcgggat aggatatcgc
    35161 aagccagaac tagattggca ccaaccagtg cgaccatgag tttggtttga cttagattat
    35221 ctccatagcg cttgcgaaca agattgggaa cgataactcc gagaaatggt aggccaccca
    35281 cggtaatcat ggtgacgctt gtcgttagcg ccaccagaaa gagggccagt ttttcaagta
    35341 gggagtagga aatccccaaa ctctcgctgg tttctttccc tagattcatg atggtgaagg
    35401 tttgggataa tttccaaacg gttatcagga tgatgaggcc taagaagagc cactcatact
    35461 gatgggtctg aatcatggag aaggagccct gggtccaggc agtcatactc tgaaccagat
    35521 tgaaacgata ggcgataact tctgtgactg agccgataat cccgctatag atgatcccaa
    35581 tcagaggcaa catccacctt tcctttacag taaaaatggt cataaaggct aggaagaaga
    35641 gggtgaatac gatggatgaa acaaaagcga agagcatctt ttgggtcaga ctagccgatg
    35701 gaaagacaaa aaggctcagc accattccca gtttggcggc ttcagtcgtt ccaactgtac
    35761 tcggtgcagc aaactgattt tgggtaatag tctgcatgag aaggcctgcc atactcatac
    35821 tagaggcagt caggagaata ctgatagttc ttgggagacg ggactcttga aagaggagcc
    35881 aggtctgctg gtcgaaatca aatagctttc cccatgaaaa atcactggtc ccaatgctaa
    35941 tagagagaaa gactaggagt agaagtaagc caattaaata atgagaaagt ttcatacccc
    36001 gtcctttcat gtagatttgg tatcgaaaga tacctgcgga tataaatgta acattatttt
    36061 tctaatctgt caataaaatt tctgacaatt taataaatac aacaaggaga gagcaacaag
    36121 actttctcct ttgttatcct attctaaaat gtttttacct taatctgata aaataatatc
    36181 ttcgagggag tagctagccg tccaatcaag atattgttta gcttttgaag catctgctag
    36241 gacactggct gggtcactag cacgtcgagc aacaatctcg tgtgggattt tttaatttag
    36301 taattcttca gcagttttaa agatttcttt gatagtatag ccttttttag ttcctaagtt
    36361 aaagatttga gaagaactgt cttcttgaaa taggtagttc attcctttaa catgagccta
    36421 tgcaaggtcc aagacataaa tgtaatctcg aatacatgaa ccgtcacgtg tatcgtagtc
    36481 atctccaaat atttttaagc tatcattttg tcccaatgcg gtcttgttga tatttggaat
    36541 gatgtgagtt ggatttttca cacgcagacc gtttgaagca tccatttcag ccccagcaac
    36601 attaaagtaa cggaaaataa catatttcca gtcgtagcga ttggccatcc agtaaatcat
    36661 tcgttcgccc atcagttttg tctctgcata agggttgaca gggtcgagca gggtatcttc
    36721 agtcgccggc ttgtcaatac agttatttcc atagagagaa gcagtcgaag agaacatgat
    36781 tttttgaatg ccaacttcag ataagacttt gagaacttgg ttcataccag caacgttggc
    36841 agtgaagtat ttacttggat tttcaatact ttcgctcaca acaatttcac ctgcataatg
    36901 gaggacagca tcaatctgat tttcttctaa gtaagccttt aaggcgctag catcataaac
    36961 atccagttct ttaaagctag cacgactatc cacagccgct cgatttcctg tggagagatt
    37021 atcgaggaca tgcaccttat aaccagcatt taaaagagct ttaacggtat gggagccaat
    37081 gtagctagtc ccacctgtaa caagaattgt tttggtcatg attttttcct tttctagttt
    37141 tgtactatag taaattgaaa taagatatga acaactctat taggaaagtc aaattaattt
    37201 ctagaaatgt tttagcagct acagcgtact attccaaact caaccaacta tatatttaaa
    37261 gaaagaagaa agggaagtca actattttct gtaaatttca tgaaaaatcc aaacaacttt
    37321 gatggtgtat tgtttttttg tttggaaata aatattcgag ttaattttgg aatgtttact
    37381 agattttgca ctacatgcaa gagaatacta tccttttcta agatagagca ggactaagat
    37441 actaacttag aaatccacct tcccactatc cttctcctat aaaaagtggt aaaatggatg
    37501 aaagaaagaa ggaactgaaa tgacaacatt attttcaaaa attaaagaag taacagaact
    37561 tgctgcagtc tcaggtcatg aagcgcctgt ccgtgcttat cttcgtgaaa agttgacacc
    37621 gcatgtggat gaagtggtga cagatggctt gggtggtatt tttggtatca aacattcaga
    37681 agctgtggat gcaccgcgcg tcttggtcgc ttctcatatg gacgaagttg gttttatggt
    37741 cagcgaaatc aagccagatg gtaccttccg tgtcgtagaa atcggtggct ggaaccccat
    37801 ggtggttagc agccaacgtt tcaaactctt gactcgtgat ggtcatgaaa ttcctgtgat
    37861 ttcaggttct gttcctccgc atttgactcg tggaaagggg ggaccaacca tgccagccat
    37921 tgccgatatc gtttttgatg gtggttttgc ggacaaggct gaggcagaaa gttttggcat
    37981 ccgtcctggt gataccattg taccagatag ttctgcaatt ttgacagcca atgaaaaaaa
    38041 tatcatctca aaagcttggg ataaccgcta cggtgtcctc atggtaagcg agctagctga
    38101 agctttatcg ggtcaaaaac tcggcaatga actctatctg ggttctaacg tccaagaaga
    38161 agttggtctg cgtggcgctc atacctctac aaccaagttt gacccagaag tcttcctcgc
    38221 agttgattgc tcaccagcag gtgatgtcta cggtggtcaa ggcaagattg gagatggaac
    38281 cttgattcgt ttctatgatc caggtcactt gcttcttcca gggatgaagg atttcctttt
    38341 gacaacggct gaagaagctg gtatcaagta ccaatactac tgtggtaaag gcggaacaga
    38401 tgcaggtgca gctcatctga aaaatggtgg tgtcccatca acaactatcg gtgtctgcgc
    38461 tcgttatatc cattctcacc aaaccctcta tgcaatggat gacttcctag aagcgcaagc
    38521 tttcttacaa gccttggtga agaaattgga tcgttcaacg gttgatttga ttaaacatta
    38581 ttaaaccata agggaggtgg tagggatggc agaaccaaac ctagaaagcc ttataaaaga
    38641 tctttacaat catgctcgac atgatttgag tgaagattta gttgctgctc tcctagagac
    38701 tactaaaaaa ctgcctacta caaatgagca attgcaggca gttcgtctct caagcctggt
    38761 caatcgtgaa ttgctcctaa atcccaaaca tccagcacct gagttgctca acttggctcg
    38821 ctttgtcaaa agagaagaag ccaagtacag aggaactgcg acttctgcgc ttatgtatga
    38881 ggaacttttt aaaatgcttt gattccattg tggatcaaag cattttttat aagacctatc
    38941 tcgtgagcgt tcaaatagta gtaacaagga atcatgagaa gtcagccgag accatagtag
    39001 tgaagaaact tctgtaatgg aagtggagtg aagtgaggaa tcgtcaaacg gtaggggata
    39061 ggctgtttga agggtgctac gaaaaagata gggtaaggtg aaattatagc ggaatgaaat
    39121 aagatatgaa caaattgatt agggaaatca aatgaattta tagaaatctt ttagcagtca
    39181 tggcgtccta ttctagtttc aaaatgctat aaatacgaac aaattgattg ggaaattcaa
    39241 atcgctttct atcaatgttt tagaagatac agtgccctag tccaacttca atatactata
    39301 aaagaacaga tggaagcaat tatgatttac ttctatcttt aaaataaatg atctgatagg
    39361 aaaaatgtgt cccctcataa aaaataggat agtacggatg gaaggctttt gtagactgga
    39421 ggacgaagga gtttagaaac tctcgttaaa agttagttga gccggagtat ggtggtgtgg
    39481 ataattatca aaaattattt ctaaatcgaa atagttgaca gttagaacga tgagtgttac
    39541 aattgtttta ttcatttcga tatcgaaata aattggaggt gttatgaaag atggtcattt
    39601 gctagcccat catattcgtt tgttgaatgg gcggattttt caaaagttac tgagtcaaga
    39661 tcctgaggct ctttataggg gtgaacaggg caagatttta gcggttttat ggaatagtga
    39721 aactggctgc gcaactgcga cagatatcgc gcttgcgact gggcttgcga ataatacgct
    39781 aacgactatg ataaaaaagc tagaggaaca aaagcttgta attgttagtc cgtgtggaaa
    39841 agacaagcgt aagaagtatt tagttttaac ggagttaggc aagtcccaga aagaagtggg
    39901 gcatcgtgtc agtcagaaat tggatactat cttttacaaa ggattttcag aggaagaaat
    39961 tcaccaattt gaaggttttc aagaaagaat tttggcgaat ttgaaagaga agggaaatga
    40021 ggtttagaat atgtcacaac aagtgaaaaa tgctcacaac ctatacatcc atgccattca
    40081 agacggacga gttgctgagg ctcaagccca gtctgtaggg gatacctata tccaacattc
    40141 gacaggtgtt cctgacggga aagaaggatt tgcagccttc tttgcagatt tctttgagcg
    40201 tcatccagag cgtcagatta agattgtccg caccattgag gatggcaatc tggtctttgt
    40261 tcatgttcat caatatctga atggtggaga agctcaatgg gtgacgacgg atactttccg
    40321 tgcggatgag aatggtcgta ttgttgagca ttgggatgtc attgactact atcgaacacc
    40381 tgaaaatgat caactagatc aaatttttgg agattttgaa atcaaggatt tggacaagaa
    40441 agcagaaaat aaaaaactgg ttcgccgttt cttgacagaa attttccaaa atggggagct
    40501 agagcagtgg agtgattatg tggcagacga tttgattcag cataatcatg agattggaca
    40561 aggaagtgct gcttataaaa actatgtggc tgaatatagt gtcacttttg actttgtttt
    40621 ccaactctta ggacaaggaa actatgtggt tagctatggt cagactcaga ttgatggcgt
    40681 tgcttatgcc cagtacgata tcttccgttt agagaacggg aaaattgtgg agcattggga
    40741 taataaggaa gtcatgccta aggtagaaga cttgactaat cgagggaagt tttaaattga
    40801 ggacaaagaa tgattgaata caaaaatgta gcactgcgct acacagaaaa ggatgtcttg
    40861 agagatgtca acttacagat tgaggatggg gaatttatgg ttttagtagg gccttctggg
    40921 tcaggtaaga cgaccatgct caagatgatt aaccgtcttt tggaaccaac tgatggaaat
    40981 atttatatgg atgggaagcg catcaaagac tatgatgagc gtgaacttcg tctttctact
    41041 ggttatgttt tacaggctat tgctcttttt ccaaatctaa cagttgcgga aaatattgct
    41101 ctcattcctg aaatgaaggg gtggagcaag gaagaaatta cgaagaaaac agaagagctt
    41161 ttggctaagg ttggtttacc agtagccgag tatgggcatc gcttacctag tgaattatct
    41221 ggtggagaac agcaacgggt cggtattgtc cgagctatga ttggtcagcc caagattttc
    41281 ctcatggatg aacccttttc ggccttggat gctatttcga gaaaacagtt gcaggttctg
    41341 acaaaagaat tgcataaaga gtttgggatg acaacgattt ttgtaaccca tgatacggat
    41401 gaagccttga agttggcgga ccgtattgct gtcttgcagg atggagaaat tcgccaggta
    41461 gcgaatcccg agacaatttt aaaagtgcct gcaacagact ttgtagcaga cttgtttgga
    41521 ggtagtgttc atgactaatt taattgcaac ttttcaggat cgttttagtg attggttgac
    41581 agctctatct caacatttgc agttgtcact tttgaccttg ttactagcta ttttgcttgc
    41641 gattcccttg gctgtttttc ttcgctatca tgagaagctg gccgactggg tcttgcagat
    41701 tgcaggtatt ttccagacca tcccgtctct ggccttgttg ggactcttta ttcccttgat
    41761 gggaattggg actttaccgg cattgacagc tctagtgatt tatgcgattt ttccgatttt
    41821 acaaaatact attactgggc tgaagggaat tgatccgagt cttcaagagg ctgggattgc
    41881 ctttgggatg accagatggg agcgtctcaa gaagtttgaa attccacttg ccatgcctgt
    41941 tatcatgtct ggaattcgga cggcagcggt cttgattatc ggtacggcta ccttggcgac
    42001 cttgattggg gcagggggac tgggttcctt tatcctgttg ggaattgacc gtaataatgc
    42061 cagtttgatt ttgattgggg ccctttcttc tgcagtgcta gctattgcct ttaacttcct
    42121 actaaaagta atggaaaaag cgaaattgcg gacgattttc tctggttttg ccttggtggc
    42181 tttattactg ggtctgtctt atagtccagc ccttttggct caaaaagaga aggaaaacgt
    42241 gattattgct gggaaaatag gcccagaacc agaaattttg gctaatatgt ataaattgct
    42301 gattgaagaa aataccagca tgactgcgac tgttaaaccg aattttggga cgacaagttt
    42361 cctttatgaa gctctgaaaa aaggtgatat tgacatttat cctgaattta ccggtacggt
    42421 gactgaaagt ttgcttcaac catcacccaa ggtgagtcat gagccagagc aggtttatca
    42481 agtggcgcgt gatggcattg ccaagcagga tcatctagcc tatctcaaac ctatgtctta
    42541 tcaaaatacc tatgctgtag ctgttccgaa aaagattgct caagaatatg gcttgaagac
    42601 catttcggat ttgaaaaaag tggaagggca gctgaaggca ggctttacgc ttgagtttaa
    42661 cgaccgtgaa gatggaaata agggcttgca atcaatgtat ggtctcaatc tcaatgtggc
    42721 gactatgcag ccagctcttc gttatcaggc aattcagtca ggggatattc aaatcacgga
    42781 tgcctattcg actgatgcgg aattggagcg ttatgattta caggtcttgg aagatgacaa
    42841 gcaactcttc ccaccttatc aaggggctcc actcatgaaa gaagctcttc tcaagaaaca
    42901 cccagagttg gaaagagttc ttaatacatt ggctggtaag attacagaaa gccagatgag
    42961 ccagctcaac taccaagtcg gtgttgaagg caagtcagca aagcaagtag ccaaggagtt
    43021 tctccaagaa caaggtttgt tgaagaaatg atgggacaga gactttaaac atactgaact
    43081 caacttcctt aaacgactag ataaaagaat gctcagacaa tttcgtctgg gcttttttga
    43141 tgtgagaatg actgaatgtc acttaaaatg gaaagaaaga gaaaattttc atgattttct
    43201 aaaattttac tgtaaagaca gttgactatt cataaaatag gtgtataata ttttgtgaac
    43261 tacttaacaa ataagaggtt tcagctcatg tcgactaagg ttagaaatct tgtgtatagg
    43321 cagttcatta tctaatagag cgttgcgtcc gaaagtctat ccagacacgg ctctttaaaa
    43381 acaaaaggag aaatgatgca tacttatttg caaaagaaaa ttgaaaatat caaaacaacc
    43441 ctaggtgaaa tgtcaggtgg ttaccgtcgt atggttgcgg ctatggctga tttaggattt
    43501 tcaggaacta tgaaggctat ctgggatgac ctctttgccc atcgtagttt tgcccagtgg
    43561 atttatttgc tggttttagg aagttttcct ctctggctgg agttggttta cgaacatcgt
    43621 attgttgact ggattgggat gatttgtagc ttgacaggga ttatctgtgt aatctttgta
    43681 tcggaaggtc gagcaagtaa ttatcttttt ggcttgatta actctgttat ttaccttatt
    43741 ttggccctac agaaaggctt ttatggtgag gtgctgacga cactttactt cacagtcatg
    43801 cagccaattg gacttctagt ttggatttat caggcacagt ttaagaagga aaagcaggag
    43861 tttgtcgcgc gtaaactgga cggcaagggc tggacaaagt atctttccat tagtgtgctt
    43921 tggtggttgg cctttggctt catttatcag tctattggtg ccaatcgtcc ctatcgtgat
    43981 tcaatcacag atgcaaccaa tggggtaggg caaatcctca tgacagctgt ttaccgtgaa
    44041 cagtggatat tctgggcggc taccaatgtt ttttcaatct atctctggtg gggagaaagc
    44101 ctgcaaattc aagggaaata tctaatttat ctcattaaca gtctagttgg ttggtatcaa
    44161 tggagcaagg cagctaagca gaatactgat ttacttaact aggaaaagat gtttgaaagt
    44221 gctgttttga gattttgatt aaaacagata tagttgataa tcaaggattt atagtatgaa
    44281 aaagaggatc ggcgggtcct cttttgttgt tgaaaagata aaaaactcag taacctagaa
    44341 ataagacaac tgaagcttta ctctatattc aatttttagg aatgagaagg tctagataaa
    44401 attggacaac ttcctggtct gtgaaatctt gccctttttt gagccaccag gtcaatgtct
    44461 cgataaagtt ggacatgacc aagtgttgga ggtaagaagt aggcagatta gggtgggctt
    44521 cttttaaatt atcagctagc acggaataga catggtgttc tagctcttta tggagttgac
    44581 ggaggaagta gtcatttttg gaaaatagca gactggtgat atggtcttgg tttttatgaa
    44641 aatggagaaa gaggtgggcg aggtagtcct cggttgaaat ggcttgctct ctttcaaaaa
    44701 gatgatggaa gaggtagcgg cagagctggt ccagaagaag ctccttactc tcatagtgac
    44761 agtaaaaggt ggatcgtccc acatctgcga gatcaatgat atcctgaaca gtagtggcct
    44821 cgtagccctt agcattcaaa agttgtataa aagcttgata gatggctttt ttggttttgc
    44881 tgatacggcg gtcaatgtta gtcatatgga cacttaaggc aaattgttca gaactgaata
    44941 aagctgacgt tttgcttcta tcctttcttt gagttttagt ggataatgat aatgaacaag
    45001 gtgttcataa atctattata acaaaggaat gagaaatatg aaggcaaaat atgctgtttg
    45061 ggtggctttt ttcttaaatt tgacttatgc cattgttgag tttattgcag gtggagtatt
    45121 tggttctagc gctgttcttg ctgactctgt gcatgacttg ggagatgcga ttgcaattgg
    45181 aatatcagct tttctagaaa caatctccaa tcgtgaagaa gacaatcagt acaccttggg
    45241 ctataagcgg tttagcctgc taggagcctt ggtaacagct gtgattctcg taacgggctc
    45301 tgttctagtc attttggaaa atgtcacgaa gattttgcat ccgcaaccag tcaatgatga
    45361 ggggattctc tggttaggaa ttattgcgat tactatcaat ctgttagcga gtctggtggt
    45421 tggtaaggga aagacaaaga atgagtctat tctgagtttg cattttctgg aagatacgct
    45481 agggtgggta gctgttatcc tgatggcgat tgttcttcga tttacggact ggtatatcct
    45541 agatcctctt ttgtcccttg tcatttcttt ctttattctt tcaaaagccc ttccacgttt
    45601 ttggtctaca ctcaagattt tcttggatgc tgtgccagaa ggtcttgata tcaagcaagt
    45661 aaagagtggc ctggagcgat tggacaatgt ggccagcctt aatcagctta atctctggac
    45721 tatggatgct ttggaaaaaa atgccattgt acatgtttgt ctaaaagaaa tggaacatat
    45781 ggaaacttgt aaagagtcta ttcgaatttt cctaaaagat tgtggttttc aaaatattac
    45841 cattgaaatt gatgctgacc tagaaactca ccaaacccat aagcgaaagg tgtgtgactt
    45901 ggaacggagt tatgagcatc aacattagaa aaaagtgaaa aatacttggg tactatctta
    45961 tttggaatag agtaatttct ttattattta aatatttcaa aaattggtaa gagaagagca
    46021 ttgtataaac tccagatata tgattgttaa tgataaaaat ttttcgatta gatacaaaat
    46081 gcttgacttg gagtcaactc aaagttatat aataagataa gtgagttaga atagcgtgaa
    46141 ttcagtgaat gaaatgagag gaggttagcg tgtgaatatt aaatctgcca gtgatttgtt
    46201 gggaatttca gcggatacga ttcggtatta tgaacgggtt ggtcttgtgc caccgattac
    46261 tcgtactgct actgggattc gtgattttca agatcaggat atcgaagcgc tggaatttat
    46321 taagtgtttt cgttcggcgg gtgtctctgt agatagttta gttgactata tgtcgctcta
    46381 ccaaaaggga gatgaaacga gagaggagag gcttggtatt ttagaagagg aaaagcaaaa
    46441 attagaggag cgcttgtctc agctacagac agctttaaat cgtttaaatc tcaaaattaa
    46501 actttataag gaaggaaaat tttaaatgaa atcagcagta tatacaaagg caggtcaggt
    46561 tggacttgct agcattgaac gtccgcaaat aatagaagcg gatgatgtga ttattcgtgt
    46621 ggttcgtgcg tgcgtttgtg gttcagattt atggaggtac cgtaatccag aaacgaaagc
    46681 tggacataaa aatagtggac acgaagcgat tgggattgtt gaagaagctg gggaagccat
    46741 tacgacggtg aaaccaggtg attttgtgat tgtccctttt acacatggat gtggtgagtg
    46801 tgatgcctgt cttgctggat ttgacggttc ttgcgacaat catattggca ataatttggg
    46861 gggtgatttt caggcagaat atattcgctt ccactatgca aactgggcgc tggttaaaat
    46921 ccctggtcaa ccttctgatt atacagaagg gatgctcaag tcccttttga ctcttgcaga
    46981 tgtcatgccg acaggctatc atgcagcgcg tgttgcaaat gttcaaaaag gggacaaggt
    47041 tgttgttatc ggtgatgggg ctgttggtca atgtgctgtc atcgcggcta agatgcgtgg
    47101 agcatcacaa attatcctta tgagccgtca tgaagaccgt caaaagatgg ctatggagtc
    47161 aggtgcgaca gctgttgttg cagaacgtgg tcaagaagga attaccaagg tgcgtgaaat
    47221 cctcggtgga ggagcagatg cagcacttga atgtgttggt acggaggctg ctatagaaca
    47281 ggcgctaggt gttcttcata atggagggcg tatgggcttt gtaggagtcc cacactataa
    47341 taatcgtgct cttggttcga catttatgca aaatatctct gtagcaggtg gggcagcttc
    47401 tgctacaaca tacgataagc aatttttact aaaagccgtc cttgatggtg atatcaatcc
    47461 aggtcgcgtc tttacttcaa gttataaact ggaagatatc gaccaagcct acaaagatat
    47521 ggatgaacgt aagaccatta agtctatgat tataatcgaa taaaaaacga ataggagttc
    47581 taaaactcct attcgttttt tatgttatcc tattcttgat ttagggtact ttctcttaat
    47641 gtcagtctgg ttcccagcat ggtcaggcta gggattttcc gaccgtggag gacttccttg
    47701 ttaagaatat ccatacctgc tcggcccatt tcttcagtat aaactgtaat actagagagg
    47761 ggaggataga cctgtttggt cagactagtg tcgttaaagg aaatgaggct gacgcgatct
    47821 ggcaggttga ttccagcttc ttggagggca cggagggcac cgatagctaa actatcgctg
    47881 gctgcgaaaa atgctggcgg aagttggtct cccaagctct gaatggcctc cttcatcaag
    47941 tcatagccag actgggcagt aaagtttcct tgaaagatca gttcatcatg atagattcct
    48001 tttgcttgac tgtagtttct gaaattttcc agccgcttat cctcaatgat ttcttcttgg
    48061 tctgttgttt cttcaaggcc tgttagaatc ccgatacggt ccattccttg actgaggaaa
    48121 taatcgacaa cctgtttcat agcagtgtaa aaatccgtga taatacaggt atgtcccagg
    48181 gaaagtgtat cgctgtctag aaatacaaga ggcttttggt attcttcaaa ggcagaaatc
    48241 tgagctcgac taaactttcc gatgcagaga atcccaatca cttcctcgct tagggtaaaa
    48301 gggtggtcat taaaatagcg caagatatcg tagtccaact cttgggctct tttttctatt
    48361 cctaggcgaa tctggtagta gtagaggtcg tctagctccc cttgttcgct gacccattga
    48421 ataatggcaa tcttttgctt gggtttgtgg gactcgcctg tcttgaggtg cttggtgtag
    48481 cccagctctt cagcaacggt taaaatacgg tgtctggttt cttctgtaac agataggctc
    48541 tggtcgcggt tgaggacacg ggatacggtc gcgattgaga cagaggctag ctgtgcaatg
    48601 tcttttaagg tagccataaa tcctccttga ttaggttagt atatcatatt tttcttcttt
    48661 ttactgatat tttagtaaaa ttttagtaaa aaggattgac cttgggaaat tccttggata
    48721 caatagaaag aaaacgatta cacgttaaga tggcttaacg gacagtcaaa ggagaattca
    48781 tatggcacaa catcttacta ctgaagtcct tcgcaaagac tttcttgctg tttttggtca
    48841 agaagcagac caaaccttct tttcaccagg ccgtattaat ttgattggtg aacacacaga
    48901 ctacaacggt gggcacgttt ttcctgctgc tatttccttg ggaacttacg gtgcagctcg
    48961 taagcgtgac gaccaagtct tgcgtttcta ctcagctaac tttgaggaca agggcattat
    49021 cgaagtgcct ctcgctgacc tcaagtttga aaaagagcac aactggacca attatccaaa
    49081 aggggttctt catttcttgc aagaagctgg gcacgtgatt gacaaaggtt ttgattttta
    49141 tgtttatggg aatatcccaa atggtgctgg cttgtcttct tcagcatcct tggagctctt
    49201 gacaggagtc gtggcagagc atctctttga tttaaaacta gaccgtttgg atttggttaa
    49261 aatcggaaaa caaacagaaa acaactttat cggagtcaac tctggtatca tggaccagtt
    49321 tgctattggt atgggggctg accaacgtgc tatttactta gataccaata ctttagaata
    49381 cgacttggtg ccacttgatt tgaaggacaa tgtcgttgtt atcatgaaca ccaacaaacg
    49441 ccgtgaattg gcggactcta aatacaatga acgtcgtgct gagtgtgaaa aagcagtgga
    49501 agaattgcaa gttgccttgg atattcagac cctgggtgaa ttggacgagt gggccgttga
    49561 ccaatatagc tatttgatta aagatgaaaa tcgtttgaaa cgtgctcgcc atgctgtgct
    49621 tgaaaaccaa cgtaccctta aagctcaagc agcccttcaa gcaggtgatt tggaaacatt
    49681 tggtcgtttg atgaatgcgt cacacgtttc tctggagcat gattatgaag taactggttt
    49741 ggaattggat actcttgttc acacagcttg ggcacaagaa ggagttctcg gtgctcgtat
    49801 gacaggggct ggttttggtg gctgtgccat tgccttggtt caaaaagata ctgttgaggc
    49861 ctttaaggaa gctgtaggca aacactacga ggaagtagtt ggatacgctc caagcttcta
    49921 tatcgctgaa gttgcaggtg gcactcgcgt ccttgactag tcaaaaggag gctctatagt
    49981 gatcttagta gataaatttg taacacatgt catttctgaa agttcatttg aggaaatgga
    50041 tcgaatctac ctgaccaacc gtgttttggc acgagtggga gaaggtgttt tggaagttga
    50101 gaccaatctg gataaattga ttgacctcaa ggaccagcta gttgaggaag ccgttcgatt
    50161 agagatgatt gaggatagtc agactgcgcg tgaaatcctt ggtactgaac tgatggactt
    50221 ggtgactcct tatccgagtc aggtcaatcg tgacttctgg gaagcctatg tccactctcc
    50281 tgagcaagcg atagaggatt tttatcaact cagtcagaaa aatgattaca tcaaactcaa
    50341 ggccattgct aaaaatatcg cttatcgtgt tccatctgat tatggagaac ttgaaattac
    50401 catcaatctc tctaagcctg aaaaggatcc gaaagagatt gcggtagcca agttggtgca
    50461 agctagtaat tatcctcagt gtcaactttg tttagagaat gagggttacc atggtcgggt
    50521 caaccaccca gctcgcagca accaccggat tatccgtttt gaaatggttg gtcaggaatg
    50581 ggggttccag tattcgccct atgcttactt taatgagcat tgtatctttt tagatggcca
    50641 gcatcgtccc atggccatta gtcgtcagag ttttgaacgt ctgttggcta tcgtagagca
    50701 gtttccagga tattttgctg gatctaatgc cgacctgccg attgtggggg gctctattct
    50761 aactcacgat cattatcagg gaggccgtca cgtatttcct atggaattgg ctcccttgca
    50821 aaagaccttc cgatttgctg gttttgagca ggtcaaggct ggaattatca agtggccaat
    50881 gtctgtccta cgcttgactt cggattccaa agaggatttg attaacttag ctgataagat
    50941 tttccaggaa tggcgtcagt attcagattc tagtgtgcag attttagcag agacagacgg
    51001 gacaccgcat cacaccatca caccaatagc gcgtaagcgt gatggacagt ttgagttgga
    51061 cttggtcttg cgagacaatc agacttcagc agagcatcct gatggcatct atcatcccca
    51121 caaggatgtc caacatatca agaaggaaaa tatcggcttg attgagatca tgggcttggc
    51181 aatcttgcca ccacgtctga aagaagaagt agagcaagtc gctagctatc ttgtaggaga
    51241 agctgttaca gttgccgatt atcatcagga gtgggcagac caactcaaat cccaacatcc
    51301 agacctaacg gataaagaaa aagcccttgc aatcgtcaag gactctgtgg gtgctatctt
    51361 tgcgcatgta cttgaggatg caggagtcta caagcagaca gaacaagggc agacagcctt
    51421 tatgcgcttt gtggaacatg tcggaatttc actagactag gagctttctc cttgcacagt
    51481 cctataaaaa gtagtatcat agtgtcgttt gaaatatcag gaggaaacga tgaaagactt
    51541 tcattttgac gctatatctg cctttgaaaa ttacgaaatt gaaaaaatga gagatggtca
    51601 cgttgtggtg acgaccaaag tagtgaactc gtcgctcaac tactatggca atgcccatgg
    51661 tggctatcta ttcacgctat gtgaccaaat cagtggtttg gtggttatct cgctgggact
    51721 tgatggggtg acactccaat cctctatcaa ctaccttaag gcaggaaaac tcgacgatgt
    51781 attgaccatt aaaggagaat gtgtccatca aggccgtaca acctgtgtga tggatgtgga
    51841 tatcaccaat caagagggca gaaatgtctg taaagcaacc tttaccatgt ttgtcacagg
    51901 ccaaaggtct gaggaaagac gggtaaggat ataaagagta atttaataga gtgaggtgaa
    51961 ttttggaatt tataagattt gccttactca ttttttgata ttgatatgat ttaattctaa
    52021 aatagaaaat ttagtaatgt ggtagaaaat acaagagttg tgttttaatt tctatggtat
    52081 aattaaaagc atgaagataa aagaaataaa gaaagttact ttacaaccgt tcacgaaatg
    52141 gacaggtggt aaaagacaat tattgcctgt tattagagaa ttaatgccta aaacctataa
    52201 caggtatttc gaaccttttg ttggaggtgg agctttattt tttgatttgg ctcctaaaga
    52261 tgcagttatt aatgatttta acgctgaact aataaattgc tatcaacaaa ttaaggacaa
    52321 tcctcaagaa ttgattgaaa ttttgaaagt tcatcaggaa tataattcaa aagaatatta
    52381 tttagattta cgttctgcag atcgtgatga aagaatagat atgatgtccg aagtacaaag
    52441 agctgcacgt attctatata tgttgagagt gaactttaat ggtctatatc gtgtgaattc
    52501 taagaaccaa tttaatgttc catatggacg ttataagaat cctaaaattg ttgatgagga
    52561 attgatatct gctatttcag tttatctaaa taacaatcaa ctagaaatta aagtgggaga
    52621 ttttgaaaag gcaattgtag atgttcgaac aggagatttt gtgtattttg accctccata
    52681 tattccattg tctgagacga gtgcatttac gtcttatact catgagggat tctcttttgc
    52741 agatcaagta agattaagag atgcctttaa gagattgagt gatacaggag cttatgttat
    52801 gttatcaaat tcttctagtg ctttagtaga ggagttgtat aaggatttta atatacatta
    52861 tgttgaagct acccgaacta atggagcaaa atcttcaagt cgaggaaaaa tttctgaaat
    52921 tatagtcaca aattatgaaa aataacgaat ataagtatgg aggtgttctt atgacaaaac
    52981 catactacaa taaaaataag atgattcttg ttcattcaga tacgttcaag ttcttatcaa
    53041 aaatgaaacc agaaagtatg gatatgattt ttgctgatcc accttatttt ttaagtaatg
    53101 gtggaatatc taattctggg ggacaagttg tttctgttga taaaggagat tgggataaaa
    53161 tttcttcatt cgaagaaaaa catgagttta atcgtaaatg gattcgccta gcaaaagaag
    53221 ttctgaagcc taatgggacg gtatggattt caggtagttt gcacaacata tactcagttg
    53281 gaatggcatt agaacaagaa ggttttaaaa ttctgaataa tattacttgg cagaaaacaa
    53341 accctgcccc caatttatct tgtcgttatt ttacccattc tactgaaacc attttatggg
    53401 ccagaaaaaa tgataaaaaa gctcgtcatt actacaatta tgatttaatg aaagaattga
    53461 atgatggaaa acaaatgaaa gatgtctgga ccggttcttt aacaaagaaa gttgaaaaat
    53521 gggctgggaa acatccaact caaaaaccag agtatttgtt agaacgcatt attttagcct
    53581 ctactaaaga gggtgactat attctagacc catttgttgg tagtggcact acgggtgttg
    53641 ttgcgaagcg gttaggtaga agatttatag gtatcgatgc tgaaaaagaa tatttaaaaa
    53701 ttgcaaggaa gaggttggag gcagaaaatg aaacaaacta gaaattttga tgagtggttg
    53761 tcaacgatga ccgacacagt agctgactgg acttattata cagattttcc taaggtttat
    53821 aaaaatgtat ctagtataaa agttgctcta aatattatga atagtcttat tggtagtaaa
    53881 aatatacaag aggatttttt agacttatat caaaattatc cagaaatttt gaaagttgtg
    53941 cctctactta ttgctaaacg tttgagagat acgattattg ttaaggatcc gataaaagat
    54001 ttctattttg attttagtaa acgaaactat agtattgaag aatatactat gtttttggaa
    54061 aaatcaggta tatttgattt attgcaaaat catttagtat caaatttagt tgactacgtt
    54121 acaggtgttg aggttggtat ggatactaat gggcgcaaaa atcggactgg agatgcgatg
    54181 gaaaatattg ttcaaagtta tcttgaagct gaagggtata ttttaggaga gaatttattt
    54241 aaagagattg aacaaaatga aatagaggaa attttttctg tcgatttatc tgctattacg
    54301 aatgatggga atactgtgaa acgttttgat tttgtcataa aaaatgaaca agtgctttat
    54361 ctgattgagg ttaattttta ttcaggaagt ggttcaaaat taaatgagac ggctagatct
    54421 tacaaaatga ttgctgaaga aattaaagct attcctaatg ttgagtttat gtggattact
    54481 gatggacaag gttggtataa ggctaagaat aatttacgag aaacatttga tattttacca
    54541 ttcttatata atattaacga tttggaacat aatattttaa agaatttgaa ataggtccaa
    54601 ttcaattaga tgggaattgg gattgattgt atttataatc aatttgtgat aaacaaaaaa
    54661 gaggctcgca cctctttttc ttatttcttt ttatgattta atacggcatt gaggacaata
    54721 gcgagtaggc tggctacgac gattccgttt gagaagaaca tttggaaggc tgttggcatg
    54781 ctgacaaaga gattactgtt gttgagaccg acacctgcag cgattgaaac agctgcgata
    54841 aggaagttgt gttcattgtt agcaaagtca actcgggcga ggatttgcat cccttgaata
    54901 gatacaaaac caaacatcac cagcatggca ccgccgagga cggagcttgg aatgatttgg
    54961 gcaagggcac caaacttagg aagcagtcca aggagaacca ggaaaccagc tgcgtagtag
    55021 attggcaggc gttttttgat gcctgacaat ttaaccaaac caacgttttg tgaaaatccg
    55081 gtgtaaggga aggtgttaaa gattcctccg agaagtacgg ccaaaccttc tgcgcggtat
    55141 ccgttgcgaa ggcgcgtgct gtcgattgga tcctttgtga tatcagacaa ggctagataa
    55201 acaccagttg actcaaccat agacaccgtt gcgatgatac acatcatgac aatagatgag
    55261 atttcaaagg ttggcatccc aaagtagagt ggaattggga catggacaag tggagctacc
    55321 gcaacaggag agaagtccac caagcccata gtagcagcaa tggcagttcc aacaaccaga
    55381 ccaatcaaaa tagagataga cttgataaat cctttggtaa agatattgat caagaggata
    55441 atcagaacag taatagctgc aagcaagaga ctttgaccag ttggctctgg aacgttattt
    55501 cccatatttc caatagcgac agggatcaag gttaaaccaa tcgtggtaat aacagatcct
    55561 gttacgatag atgggaagag attggctact tttgagaaga tgcctgaaac aagaaccacg
    55621 taaatcccag atgcgataag ggcaccaaac atagcgccac taccatggct ttgcccaatc
    55681 ataatcaagg gagcgaccga ctggaatgca actccaagaa cgactgggag tccaatccca
    55741 aagtatttgt tgagttggag ttggaggaag gttgccaccc cacacatgaa gatatctgta
    55801 gaaatcaggt aggtcaactg ctcagctgaa tagccaaggg ctgtcgcaat catgatggga
    55861 accaggatag atcctgagta catggctagt aagtgctgca agcccagaac ggctgcttgc
    55921 gagtgttttt cttgagtttg cattagagat ctgcctcctt aaatacgacc tgaccatttt
    55981 caaaacgatc caaacgagca agtgatagga cagggtagcc tgctttttca agcaaatcac
    56041 gaccatcttg gaaggatttc tcaatcacga taccgatagc ttggactgtg gcaccggcct
    56101 gttcgatgat ttgaatcaag cctttagcag cttggccatt agcaaggaaa tcgtcgataa
    56161 tcaaaacctt gtcctctggt gagaggaatt ttccagcgat agaaacagtg ctggtcacct
    56221 gcttggtaaa ggagtagact tgagcagtta agatgccttc gttcatggtg atgttcttag
    56281 cttttttggc gaaaatcatg ggaacgttta aggcttcagc tgtaaaaacg gctggggcaa
    56341 tacccgacgc ttcaatggtt acgaccttgg taatgccagc agcagcaaat ttttccgcaa
    56401 aaaccttacc aatctctcgc atcaagctaa agtcaacttg gtgggttaaa aaggaatcta
    56461 ccttgaggat gttatcaccc aagatatgcc catccttgag gatgcgctct tctaataatt
    56521 tcataagacc tcctaaagtc taaaagttaa tttacttgtt gtttaaatgt ttctatagtg
    56581 atcccttttg ctaatattat atatttgata aaactattac gagcgaagcg agtcttatca
    56641 aatatttccc gttgtagtgg tatcatagac aataatcttg ttattgtcta tgacgggatt
    56701 tttgagagta aaatagttcg gggaactatt ttagcctaag cctagaaatg aaagagctag
    56761 gggctcaaaa attagggatg aaattccctg gattcctgaa attattcaca ggataatttc
    56821 acctcccgtc cgcactaatt aagggaaata ttaaaaatat tcaaaaaaga cctacttaat
    56881 ctctaagtaa gtcccccaaa taggcatggc aaaaacggcc atatctcact gctgacttac
    56941 ttattgttag gtgttccggc accttgtaga aacgtcgtgc caattcacga cataaacaag
    57001 taaaacgata ttcaatttta aataggcttg agccaatgtt tttattttac actaaataac
    57061 tttaaaaatc aactattttg ttagtgtttc ggtttaaaaa acgaacaaac agaagagagg
    57121 gtgaataaaa actccattgt aagctaacat ttatactaaa tgaaaatcaa agagcaaact
    57181 aggaagctat ccacaacctc aaaacactgt tttgaggttg tggatagaat tgacagagtc
    57241 agtatcatat acctacggta aggcgacgtt gacgtggctt gaagagattt tcgaagagta
    57301 ttagaagatt tttccatcat aaaaggcata ctatcaagtt tttagacacc tgacaatatg
    57361 cctttttcta actttaaaga cttttcccaa tttttattat tctactcgct aaatcttaaa
    57421 aaatagccat ctggatccaa aactgcaaat ttatgaggat agatataggg atcactgaca
    57481 cgaaactttc ttttggtcaa gggacgataa ataggatagt ttgcctccat cactttttga
    57541 tagagttttg gaacatcctt tatgccaaag gagagattga ctccacgacc aaaggggtag
    57601 gtcagttcag ctagttgatc ctttgttccc tcctctaaca ttagttgaca ctcttcaaga
    57661 gaaagagaaa gttttcttct ggacgttggt attcaatcct aaaacccagt aaaccacagt
    57721 agaaggaccg ggactgttcg atattcgata caagaaactc gggaatgacc gcattgtagt
    57781 ccatatagaa aatccttaca agtcaatttc caagacaatc ggtgtatggt cttggcgagc
    57841 acctgagtca atcatatcag atttagtgac cttgtcagcg atacggttac ttgtgagcca
    57901 gtagtcgatt ctccagcctg tattgttgat tttagaagtt ttgctgcgtt gtgcccacca
    57961 agtgtagcgt tcaggaacat cgccatgaac atggcggaag gtatcagtaa atccagttgc
    58021 caataggttg gtaaatccag cacgttcctc gtcagtaaat ccaggtgaac ggcggttgct
    58081 agcaggattt gcaaggtcga tttcattgtg ggctacgttg tagtcaccgg tcgcaaggac
    58141 tggtttttct ttgtctagtt cagccaaata ctccgcatat ttgacatccc agacttggcg
    58201 ttcttccaag cgtttaagac catcgccagc atttggtgtg taaacttggg tcacgaaaaa
    58261 tgcatcaaat tctagagtga tgatgcggcc ttccaagtcc atggtagaag gggcaccgat
    58321 ttctgggaaa gtgatagtag gtgtaagttc tttcttataa aggaacatgg ttccagcata
    58381 gcctttacgg gcaggctctt gggaagagcg ccacgtgttt tcgtagcctg ggaagagttc
    58441 ttctaaaatt tccacgtgtt tctttgtagg tcctttggca gaaagcttgg tttcttggat
    58501 agcaatgata tcagcatttt cagcgaccaa ggtttgtagg acttcttggg acaatttggc
    58561 acgagctgag tcactagtta gggcagcgtt tagggaatca atattccatg agataagttt
    58621 cataaagtta cctttttcat tcagattata gattttatta taccaaaaaa agatctattt
    58681 ccccaacgta tggtttgaaa aattactctc tttcgtttat aattaagaat gattttatga
    58741 aagggagtga aaatacatga aattctactc ttatgactat gtactcagcc aaatcggtca
    58801 gcaaaatggt atcatggttg gctttgggat tgttctatta gctgtgacag tttttttgct
    58861 tttaaggcat accatgataa aaagggaacc gaatttcgtg agttggtcat gatttcagct
    58921 ctagccttat ttagctctgc ttttggtcag catcacgact tatcaaaaca atcaagtttc
    58981 taacaataaa tttcaaactt cacttcattt catcgaggtt gtttccaaag atttgggagt
    59041 agacaagtca gaagtctatg ttaatacttc cacaaacaca gatggcgcac ttatcaaggt
    59101 gggagatcgc tattatcgtg ccctaaacgg aagtgagcca gacaagtacc tgttagagaa
    59161 agtcgaattg tataagacag acgcaattga actggtggat gtgaacaaat gacacttaat
    59221 tatatcgaaa ttttaatcaa actggtcttg actctcaaat agttcaacaa caatgttcac
    59281 tttatgaaac gtttgattga tggtaagcca actctcctta tcaaaaatgg gaatattgac
    59341 ccataagcct gtcgttcagt tggtttgtct gcatcggatg tagccctcaa acttcgtagc
    59401 caagggattt tccagatgaa gcaagtcaaa cgagctgtgc aagagcaaaa tgggcaactc
    59461 atcgttgtgc aaatgggaga tgaaaatcct aagtatccag ttgtgactga cggtgtgatt
    59521 caagtagatg tcttggaatc gattggtcgt agcgaagagt ggttgcttga taacctcagc
    59581 aaacaagggc atgacaatgt agccaatatc tttattgctg aatatgacaa gggtgctgtt
    59641 acagtcgtaa cttatgaata agaaaaacct ggggtcttgt actcttcgaa aatctcttca
    59701 aaccgcgtca acgtcgcctt gccgtatgta ggttactgac ttcgtcagtt ctatctacaa
    59761 cctcaaagca gtgctttgag cagcctgcgg ctagtttcct agtttgctct ttgattttca
    59821 ttgagtattg gcctcaggct tccatttgca atcagaaagg gattttatgt ccattattca
    59881 aaaactttgg tggtttttca agttagaaaa acgccgttat ctagtcggga ttgtggccct
    59941 ggtcttggtt tccgtcctca atctcattcc tcctatggtt atggggcggg tcattgatgc
    60001 catcacatcg gggcaattaa cccagcagga cctccttctt agcctatttt acttgctact
    60061 tgcagccttt ggtatgtact atttgcgcta tgtgtggcgt atgtatatcc ttgggacctc
    60121 ttattgcttg ggacagatca tgcggtctcg cttgtttaag catttcacaa aaatgtcgtc
    60181 agccttttat caaacctatc ggacgggtga tctgatggca cacgcaacca atgatatcaa
    60241 tgccttgact cgtttagcag gtggcggtgt catgtctgcg gtggatgcct ctatcacggc
    60301 tctggtgact ttgttgacca tgctctttag catctcatgg cagatgactc ttgttgccat
    60361 tctcccccta cctttcatgg cctatgcgac tagtcgccta gggagaaaga ctcataaggc
    60421 ctttggcgaa tcccaaactg ctttttctga actcaataac aaggtacagg agtccgtatc
    60481 aggtatcaaa gtgaccaagt ctttcggtta tcaggcagac gagttgaagt cttttcaggc
    60541 agtcaatgaa ttaaccttcc aaaagaacct gcaaaccatg aaatatgata gtctctttga
    60601 ccctatggtt ctcttgtttg ttggttcgtc ctatgtttta acgcttttgg ttggctcctt
    60661 gatggttcag gaagggcaga ttacagttgg gaatctagtc acctttatca gctatttgga
    60721 tatgctggtc tggcctcttc tggccatcgg tttcctcttt aatactactc agcgagggaa
    60781 ggtttcttac cagcggattg aaaatctttt gtctcaggaa tctcctgtac aagaccctga
    60841 gtttcctctg gatggtattg aaaatgggcg tttggagtat gccattgaca gctttgcttt
    60901 tgaaaatgag gaaacactga cggatattca ctttagtttg gcaaaagggc aaaccctggg
    60961 cttggttggg cagacaggct ctgggaaaac gtccttaatc aagctcctct tgcgtgaata
    61021 cgatgtggat aagggtgcca tttatctaaa cggtcacgat attcgggact atcgtctgac
    61081 agaccttcgc agtctcatgg gctatgttcc tcaggaccag tttctctttg cgacttcaat
    61141 cctagacaat atccgctttg gcaatcctaa cttgcccctt tcagcggtcg aggaagctac
    61201 taagctagcc cgagtttacc aagatattgt agacatgcct caaggatttg atacgctgat
    61261 tggtgaaaaa ggagtcagtc tttctggtgg tcaaaagcaa cggttggcta tgagtcgggc
    61321 tatgatttta gaccctgata tcttgatttt ggatgattcc ttatccgccg tagatgccaa
    61381 gacagagtat gcgattatcg acaacctcaa ggagacgcga aaggacaaga caaccattat
    61441 cactgcccat cgcctgagtg cagttgtcca tgcagatttg attttagtcc tgcaaaatgg
    61501 tcaaattatc gaacgaggca ggcacgaaga cttgctagct ttggatggct ggtatgccca
    61561 aacctaccag tctcagcagc tggaaatgaa gggagaagaa gatgcagaat aaacaagaac
    61621 aatggactgt attgaagcgc ttgatgtcct atctcaagcc ttatagactc ctgacctttt
    61681 tggcactcag ctttctccta gcgacgacgg tcattaaaag tgtcataccc ctcgtggctt
    61741 cccactttat cgaccagtat ctcagcaatc ttaaccaact agccgtgacc gttttgctgg
    61801 tctactatgg tctctacatc ctacaaactg tagttcagta tgtcggcaat cttctctttg
    61861 cacgcgtgtc ttacagtatt gttagggata ttcgtcggga tgcctttgcc aatatggaga
    61921 aactgggcat gtcttacttt gacaagacgc cagcaggttc tatcgtttct cgtttgacca
    61981 acgataccga gacgattagt gatatgtttt ctgggatttt atccagcttt atctcagcag
    62041 tttttatctt tctgacaacc ctttatacca tgttggtgct ggattttcgt ttgacagcat
    62101 tagtcttgct ctttctcccc ttgattttcc ttttggtcaa tctctatcga aaaaagtcag
    62161 tgaaaatcat cgagaaaacc agaagtctct tgtcagatat caatagtaag ctggcagaga
    62221 atatcgaggg aatcaggatt attcaggctt ttaatcaaga gaagcgcctg caggcagaat
    62281 ttgatgaaat caaccaagag cacttggcct atgccaaccg ttctgtagcc ttggatgccc
    62341 tctttttgag acctgccatg agtttgttga aacttctagg ttatgctgtc ttgatgacct
    62401 attttggcta ccgtggtctt tctatcggga taacggccgg gaccatgtat gcttttatcc
    62461 agtacatcaa ccgcctcttt gaccctttga ttgaggtgac gcaaaacttt tcaacactgc
    62521 aaacgtctat ggtttctgca ggccgtgtct ttgccttaat cgatgagagg acctatgagc
    62581 cccttcaaga aaatgggcaa gccaaagtca aagaaggcaa tatccgtttt gaacatgtgt
    62641 gtttctcgta tgacggtaaa catccgattc tggatgacat ttctttctct gttaataagg
    62701 gtgaaaccat tgcctttgta ggtcatacag gttcagggaa atcgtctatt atcaatgtcc
    62761 tcatgcgctt ttatgaattc cagtcaggga gagttctctt ggatgatgtg gatatcaggg
    62821 atttcagtca agaagagctg agaaaaaaca tcggtttggt tttacaggaa cccttcctct
    62881 atcatggaac tattaagtcc aatatcgcca tgtaccaaga aatcagtgat gagcaggttc
    62941 aggctgcggc agcctttgtg gatgcagatt cctttattca agaacttcct caggggtacg
    63001 actcccctgt ttccgagcgt ggttcgagct tctctactgg gcagcgccag cttcttgcct
    63061 ttgctagaac agtcgccagc cagcctaaaa tcctgatttt ggatgaagcg acagccaata
    63121 ttgactctga aacagaaagt ttggttcaag cttctctggc gaagatgaga cagggccgaa
    63181 caactattgc tatcgctcac cgcctttcta ctattcaaga cgccaactgc atctatgtct
    63241 tggataaggg acgcattatc gagagtggaa cccatgagga cctcttgact ctgggaggaa
    63301 cctatcacaa gatgtatagt ttgcaggcag gggccatggc ctatactctt tgaaaatctc
    63361 tttaaaccat gtcagcttta tctgcaatct caaagctgta ctttgatttt cattgagtac
    63421 tagaaggaaa tccttcaaat tacagatttc tttcaccgcc ttttccattt tgtggtataa
    63481 tgaaaaatgt tgacaaatag tataataaaa acaaaggaga acagcatgct gaaatgggaa
    63541 gacttgcctg tggaaatgaa atcaagcgag gttgagtctt actaccagct tgtctctaaa
    63601 aggaagggtt cgctgatttt caagcgttgc ttggactggg ttttggcctt ggtcttactg
    63661 gttctaactt ctcccatctt tctcatcttg agcatttgga tcaagttgga tagcaagggg
    63721 ccagtgattt acaagcaaga gcgtgtgacc cagtacaacc gtcggttcaa gatttggaag
    63781 ttccgtacca tggtgacgga tgcggataaa aaaggaagtc tggtgacttc tgctaacgat
    63841 agccgtatta ccaaggttgg aaatttcatc cgacgtgtcc gtttggacga actgcctcag
    63901 ttggtcaatg tccttaaagg tgagatgtcc tttgtcggta cacgacctga agtgccacgt
    63961 tatacagagc agtatagccc tgaaatgatg gcaaccttgc tcttgcaagc aggaattacc
    64021 tctccagcca gcatcaacta caaggatgag gacaccatca tcagtcaaat gacggagaaa
    64081 ggtctgtcag ttgatcaggc ctatgtggag catgttcttc ctgaaaagat gcgctataac
    64141 ctcgcctatc tccgagagtt tagtttcttt ggggacatca aaatcatgtt tcaaaccgtg
    64201 tttgaggtac taaaataaag tagtcataag aaaatgagta cagataaaag gagcaaatca
    64261 atgccaaatt acaatattcc attttcaccg cctgatatca cagaagcaga aattgctgaa
    64321 gtagcggata ccctgcgttc tggttggatc acaacaggtc ctaaaacaaa agaactggag
    64381 cgccgcttgt ctctttacac acagacacct aagactgttt gtctcaactc tgcgacagcc
    64441 gctctggagt tgattttacg cgttttggaa gtgggacctg gtgatgaagt catcgttcca
    64501 gccatgacct atacggcttc atgtagtgtc attacgcacg tgggagcaac ccctgtcatg
    64561 gtggatatcc aagcagatac gtttgagatg gactatgacc tgcttgagca agctatcact
    64621 gagaaaacta aggtgattat cccagtagag ctcgcaggga ttgtttgcga ttatgaccgt
    64681 ttgttccaag tcgtggagaa aaaacgtgac ttctttaccg cttcaagcaa gtggcaaaag
    64741 gcctttaacc gtattgtcat tgtctctgat agtgcccacg ctttgggatc tacttataaa
    64801 ggacaacctt ctggttctat cgctgacttt acttccttct cattccatgc cgttaagaac
    64861 tttacaacgg cagaaggtgg aagtgcgact tggaaagcca atccagtgat tgatgacgaa
    64921 gagatgtaca aggaattcca aatcctttcc cttcacgggc aaactaagga tgctcttgcc
    64981 aagatgcaat tggggtcatg ggaatacgat atcgttacac cagcctataa gtgcaacatg
    65041 accgatatca tggcttcact tggtttggta caattggacc gctatccaag tttgttgcaa
    65101 cgccgtaagg acattgtgga ccgctatgat agtggttttg taggttctcg catccatcct
    65161 ttggcacaca agactgaaac tgtcgaatct tcacgccacc tctacatcac ccgtgtagaa
    65221 ggagcaagcc tagaagaacg caacctcatc atccaagaat tggctaaagc aggaattgca
    65281 agtaatgttc actacaaacc gcttcctctc ttgacagcct ataagaatct tggatttgat
    65341 atgacgaact atcctaaggc ctatgccttc tttgagaatg aaattaccct ccctcttcat
    65401 actaaattaa gcgatgaaga agtagactat atcattgaga ctttcaaaac agtttctgaa
    65461 aaagtgctaa ctttatcaaa aaaatgacaa actacagtca agcgaaagtg atcctgtccc
    65521 taaaaagtct aattgagtgt aaaaactgtt gttttcaatt gataatagtt tacacctgta
    65581 gttgaggcct ctttctcctc agagagagaa tttttatagg attttccttt cttgtgggag
    65641 tcccgtggtt tgaaataaga tgtgagcaat ttagtgtagc atttagaatc cttactagac
    65701 atcatttaga aaatctagtg tcttgttcta gttttcaatt caccctattt tttgaaagac
    65761 gtgagtttcc atgagtgaga ttgtggaaac tcgcgtcttt ttttgttttc agaatattgt
    65821 tcaaaatttt gtgcctgtct ttcatgttct agtcattctt ttgcatgata gaatttatag
    65881 catgttgata ttataataat acaaatattc tatatgttta gtgatgcttg ctatacatta
    65941 ttagatctcc tgcgagacaa tctataaaac acttgtctac gattacctat atgccctatt
    66001 ccagtatttt agaagcactg catctatttt tatcgaggtt aaatctagct tttatagaag
    66061 gtctatttaa gaaatatatt gtagtgtttt agtttcaatc cgccatatga gcgatattca
    66121 ggtaaatatc cctggcgaat gcttgtatga caaggtattt gttctttcat ttataattta
    66181 caacatatca acaaatttaa atatagtaaa tgggatattt tatattcaag ctaagaaaga
    66241 tagcatcact tttgaatgga aggctaaaga gcaaactagg aagttggcca tagatagctc
    66301 aaaaccctgc tttgaggttg tagatatagt aaaatgaaac aagaatagga caaattgatc
    66361 gggacagtta aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttatagtttc
    66421 agtctactat agaactgacc aagtcagtaa cctagactta gggcaaggcg gcactgacct
    66481 agtttgaaga gatttccgaa gagtataaat tttaatattt tcttgtgtta ttccttgaca
    66541 attcaatttg gaaaatatat gataaagata atgacagcgg tgtcattcta tctattttaa
    66601 gaaaagtaat aatcaattgt taaaaatagt aaaaaaattg gaggttctga tgaaatattt
    66661 tgttcctaat gaggtattca gtattcgtaa attaaaggtg gggacttgct cggtactatt
    66721 ggcaatttca attttgggaa gccaaggtat tttatcggat gaagttgtta ctagttcttc
    66781 accgatggct acaaaagagt cttctaatgc aattactaat gatttagata attcaccaac
    66841 tgttaatcag aatcgttctg ctgaaatgat tgcctctaat tcaaccacta atggtttaga
    66901 taattcgtta agtgttaata gtatcagctc taatggtact attcgttcca attcacaatt
    66961 agacaacaga acagttgaat ctacagtaac atctactaat gaaaataaga gttataagga
    67021 agatgttata agtgacagaa ttatcaaaaa agaatttgaa gatactgctt taagtgtaaa
    67081 agattatggt gcggtaggtg atgggattca tgatgatcga caagcaattc aagatgcaat
    67141 agatgctgca gctcaagggc taggtggagg aaatgtatat tttcctgaag gaacttattt
    67201 agtaaaagaa attgtttttt taaaaagtca tacacactta gaattgaatg agaaagctac
    67261 aattctaaat ggtataaata ttaagaatca cccttccatt gtttttatga caggtttatt
    67321 tacggatgat ggtgcgcaag tagaatgggg cccaacagaa gatattagtt attctggtgg
    67381 tacgattgat atgaacggtg ctttgaatga agaaggaact aaagcaaaaa atctaccact
    67441 tataaattct tcaggtgcat ttgctattgg gaattcaaat aacgtaacta taaaaaatgt
    67501 aacattcaag gatagttatc aagggcatgc tattcaaatt gcaggttcga aaaatgtatt
    67561 agttgataat tctcgttttc ttgggcaagc cttacccaaa acgatgaagg atgggcaaat
    67621 cataagtaag gagagcattc agattgaacc attaactaga aaaggttttc cttatgcctt
    67681 gaatgatgat gggaaaaaat ctgaaaatgt gactattcaa aattcctatt ttggcaaaag
    67741 tgataaatct ggggaattag taacagcaat tggcacacac tatcaaacat tgtcgacaca
    67801 gaacccctct aatattaaaa ttttaaataa tcattttgat aacatgatgt atgcaggtgt
    67861 acgttttaca ggattcactg atgtattaat caaaggaaat cgctttgata agaaagttaa
    67921 aggagagagt gtacattatc gagaaagcgg agcagcttta gtaaatgctt atagctataa
    67981 aaacactaaa gacctattag atttaaataa acaggtggtt atcgccgaaa atatatttaa
    68041 tattgccgat cctaaaacaa aagcgatacg agttgcaaaa gatagtgcag aatatttagg
    68101 aaaagtatca gatattactg taacaaaaaa tgtaattaat aataattcta aggaaacaga
    68161 acaaccaaat attgaattat tacgagttag tgataattta gtagtgtcag agaatagtat
    68221 attcggtggt aaagaaggaa ttgttattga ggattcaaag ggtaaaataa ccgttttaaa
    68281 taaccaattt tataatttat ccggtaagta tatatcattc atcaaatcta atgcaaacgg
    68341 gaaagaacct gttatacgtg atagcgatgg taatttcaat attgtaacgg agaatgggct
    68401 ttacaaaatt gtaacaaata atttaagtga taaaaacgaa aaagaaaaaa acaaagagga
    68461 aaaacaatct aattcaaata atgtaattga tagtaaccag aagaacggag agtttaactc
    68521 aagtaaagat aatagacaaa tgaatgacaa gatcgacaat aaacaagata ataagacaga
    68581 agaagtaaac tataaaatag ttggagatgg cagagaaact gaaaatcata ttaataaatc
    68641 taaagaaata gtagatgtaa aacaaaaatt accaaagact ggttcgaaca agattatgga
    68701 actattctta acagtgacag gaattggttt acttttgaca ctaaaagggt tgaagtatta
    68761 tggtaaagat aaataaaatt tgttcgatac aaggaagttc cgtagaaaat gaggatattg
    68821 taggttcaca aaatcagtat ttttggatta ttggtggagc tacagattta tataattcta
    68881 aagaggagat aggttattca gtctcagaag ttgtacatat tttatcagaa agtttgtcgg
    68941 ttaattgtaa agaatcaaaa actcttaaac aaatatttga aactgcttta ctcgaggtta
    69001 aagatgaaat cggtttaaac tcatatgaat taacag
//
LOCUS       DAVKHZ010000002        66621 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000002 DAVKHZ010000000
VERSION     DAVKHZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.002"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            91..6525
                     /locus_tag="VWW99_000072"
     CDS             91..6525
                     /locus_tag="VWW99_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9537984.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
     gene            7158..7634
                     /locus_tag="VWW99_000073"
     CDS             7158..7634
                     /locus_tag="VWW99_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9537985.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7672..7977
                     /locus_tag="VWW99_000074"
     CDS             7672..7977
                     /locus_tag="VWW99_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9537986.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            8025..9500
                     /locus_tag="VWW99_000075"
     CDS             8025..9500
                     /locus_tag="VWW99_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9537987.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            9805..16506
                     /locus_tag="VWW99_000076"
     CDS             9805..16506
                     /locus_tag="VWW99_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9537988.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVHYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKIVAAVQENLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(16754..17606)
                     /locus_tag="VWW99_000077"
                     /pseudo
     CDS             complement(16754..17606)
                     /locus_tag="VWW99_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17818..18177)
                     /locus_tag="VWW99_000078"
     CDS             complement(17818..18177)
                     /locus_tag="VWW99_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9537989.1"
                     /translation="MGMILMKLASILLLILTLVICIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            18269..19225
                     /locus_tag="VWW99_000079"
                     /pseudo
     CDS             18269..19225
                     /locus_tag="VWW99_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            19209..19766
                     /locus_tag="VWW99_000080"
     CDS             19209..19766
                     /locus_tag="VWW99_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9537990.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPAADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            19759..20013
                     /locus_tag="VWW99_000081"
     CDS             19759..20013
                     /locus_tag="VWW99_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9537991.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAMGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            20025..22079
                     /locus_tag="VWW99_000082"
     CDS             20025..22079
                     /locus_tag="VWW99_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9537992.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            22258..23118
                     /locus_tag="VWW99_000083"
     CDS             22258..23118
                     /locus_tag="VWW99_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9537993.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSINFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQFLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQTLQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGVLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            23329..24039
                     /gene="ccdA2"
                     /locus_tag="VWW99_000084"
     CDS             23329..24039
                     /gene="ccdA2"
                     /locus_tag="VWW99_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9537994.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIIFLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            24059..24625
                     /locus_tag="VWW99_000085"
     CDS             24059..24625
                     /locus_tag="VWW99_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9537995.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            24636..25748
                     /gene="msrB"
                     /locus_tag="VWW99_000086"
     CDS             24636..25748
                     /gene="msrB"
                     /locus_tag="VWW99_000086"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9537996.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            26016..26753
                     /locus_tag="VWW99_000087"
     CDS             26016..26753
                     /locus_tag="VWW99_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9537997.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            26750..28441
                     /locus_tag="VWW99_000088"
     CDS             26750..28441
                     /locus_tag="VWW99_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9537998.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            28557..29120
                     /locus_tag="VWW99_000089"
     CDS             28557..29120
                     /locus_tag="VWW99_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9537999.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            29142..34805
                     /locus_tag="VWW99_000090"
     CDS             29142..34805
                     /locus_tag="VWW99_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9538000.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGKSYIKGEFKGKLLGTNDNVRHSIFDL
                     EYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYLEG
                     RDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADIEV
                     HRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPYGA
                     IDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRISEE
                     EANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTKFT
                     PYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGSQK
                     RYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTEST
                     YQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAVAA
                     LRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRLGS
                     SGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDYIQ
                     FEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSREK
                     RPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGSEW
                     VDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSEYG
                     ISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAFER
                     KNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFKKV
                     EPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFETSY
                     IAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRDEG
                     HAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEELQ
                     EGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            34866..36587
                     /gene="pabB"
                     /locus_tag="VWW99_000091"
     CDS             34866..36587
                     /gene="pabB"
                     /locus_tag="VWW99_000091"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9538001.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            36707..38233
                     /locus_tag="VWW99_000092"
     CDS             36707..38233
                     /locus_tag="VWW99_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9538002.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            38320..39239
                     /locus_tag="VWW99_000093"
                     /pseudo
     CDS             38320..39239
                     /locus_tag="VWW99_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            39374..40333
                     /locus_tag="VWW99_000094"
     CDS             39374..40333
                     /locus_tag="VWW99_000094"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9538003.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            40430..41269
                     /locus_tag="VWW99_000095"
     CDS             40430..41269
                     /locus_tag="VWW99_000095"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9538004.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(41317..41487)
                     /locus_tag="VWW99_000096"
     CDS             complement(41317..41487)
                     /locus_tag="VWW99_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9538005.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            41608..42492
                     /gene="miaA"
                     /locus_tag="VWW99_000097"
     CDS             41608..42492
                     /gene="miaA"
                     /locus_tag="VWW99_000097"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9538006.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            42485..43723
                     /gene="hflX"
                     /locus_tag="VWW99_000098"
     CDS             42485..43723
                     /gene="hflX"
                     /locus_tag="VWW99_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9538007.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            43716..44339
                     /locus_tag="VWW99_000099"
     CDS             43716..44339
                     /locus_tag="VWW99_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9538008.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            44354..45283
                     /locus_tag="VWW99_000100"
     CDS             44354..45283
                     /locus_tag="VWW99_000100"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9538009.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            45304..46059
                     /locus_tag="VWW99_000101"
     CDS             45304..46059
                     /locus_tag="VWW99_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9538010.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(46113..47021)
                     /locus_tag="VWW99_000102"
     CDS             complement(46113..47021)
                     /locus_tag="VWW99_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9538011.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            47085..47354
                     /locus_tag="VWW99_000103"
     CDS             47085..47354
                     /locus_tag="VWW99_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9538012.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            47354..47734
                     /locus_tag="VWW99_000104"
     CDS             47354..47734
                     /locus_tag="VWW99_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9538013.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            47823..48050
                     /locus_tag="VWW99_000105"
                     /pseudo
     CDS             47823..48050
                     /locus_tag="VWW99_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(48090..48815)
                     /locus_tag="VWW99_000106"
     CDS             complement(48090..48815)
                     /locus_tag="VWW99_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9538014.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            48977..50818
                     /gene="typA"
                     /locus_tag="VWW99_000107"
     CDS             48977..50818
                     /gene="typA"
                     /locus_tag="VWW99_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9538015.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            50838..51092
                     /locus_tag="VWW99_000108"
     CDS             50838..51092
                     /locus_tag="VWW99_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9538016.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
     gene            51457..51756
                     /locus_tag="VWW99_000109"
                     /pseudo
     CDS             51457..51756
                     /locus_tag="VWW99_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            51839..53860
                     /locus_tag="VWW99_000110"
                     /pseudo
     CDS             51839..53860
                     /locus_tag="VWW99_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            53862..54503
                     /locus_tag="VWW99_000111"
     CDS             53862..54503
                     /locus_tag="VWW99_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538017.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            54607..55959
                     /locus_tag="VWW99_000112"
     CDS             54607..55959
                     /locus_tag="VWW99_000112"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9538018.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            55963..57021
                     /locus_tag="VWW99_000113"
     CDS             55963..57021
                     /locus_tag="VWW99_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9538019.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            57031..58230
                     /gene="divIB"
                     /locus_tag="VWW99_000114"
     CDS             57031..58230
                     /gene="divIB"
                     /locus_tag="VWW99_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9538020.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            58463..59164
                     /gene="pyrF"
                     /locus_tag="VWW99_000115"
     CDS             58463..59164
                     /gene="pyrF"
                     /locus_tag="VWW99_000115"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9538021.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPV
                     AAYHAIKDEWTQDWN"
     gene            59198..59830
                     /locus_tag="VWW99_000116"
     CDS             59198..59830
                     /locus_tag="VWW99_000116"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9538022.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSDLIHLAQEEGYITPEGLYLLKRFKEDQENWQEG"
     gene            60010..60414
                     /locus_tag="VWW99_000117"
     CDS             60010..60414
                     /locus_tag="VWW99_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538023.1"
                     /translation="MKRRKIYSLFCVVTIASSLFLPTVLARQIGDYDLTLPRWGSDTT
                     SELEKENASAGINNNDSTGGGKTLNTSIRSAYSGSDITPVYSLGSGSRIVMYYNGGGD
                     NYIGSGTRLAMAPQFGNHVRIHTSGSWSPDSY"
     gene            60510..61274
                     /locus_tag="VWW99_000118"
     CDS             60510..61274
                     /locus_tag="VWW99_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538024.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            61274..62026
                     /locus_tag="VWW99_000119"
     CDS             61274..62026
                     /locus_tag="VWW99_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538025.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            62036..62734
                     /locus_tag="VWW99_000120"
     CDS             62036..62734
                     /locus_tag="VWW99_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538026.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            62731..63378
                     /locus_tag="VWW99_000121"
     CDS             62731..63378
                     /locus_tag="VWW99_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538027.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(63465..64331)
                     /locus_tag="VWW99_000122"
                     /pseudo
     CDS             complement(63465..64331)
                     /locus_tag="VWW99_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(64343..65101)
                     /locus_tag="VWW99_000123"
     CDS             complement(64343..65101)
                     /locus_tag="VWW99_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9538028.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(65102..65779)
                     /locus_tag="VWW99_000124"
     CDS             complement(65102..65779)
                     /locus_tag="VWW99_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9538029.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTTEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(65739..66419)
                     /locus_tag="VWW99_000125"
     CDS             complement(65739..66419)
                     /locus_tag="VWW99_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9538030.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSRK"
BASE COUNT        21091 a        11669 c        14423 g        19438 t
ORIGIN      
        1 ataatactta gtaaaacttt ttataacaaa ggctagcaaa gttaaagttt attatctatt
       61 ggaagttaaa taaatatgta aggaattaaa atgaaaaaaa gtacagtatt gtcattaacc
      121 acagctgcag ttattttagc agcctatgcc cctaatgagg tagtcttagc agacacatct
      181 agctctgaag atgctttaaa catctctgat aaagaaaaag tagtattaga taaggaaaca
      241 gaaaataaag agaaacatga agatattcat aatgctatag aaacttcaaa ggatactgaa
      301 gaaaagaaaa caacagttat tgaggaaaaa gaagttgtta gtaaaaatcc tgtgatagac
      361 actaaaacta gcaatgaaga agcaagaatc aaagaagatt ccaatcaatc ccaaggagat
      421 aatgcacact cgtctgcaaa taaaggcaca gaaaatccga aaaaagaaga tagacttgtc
      481 tatattgctg aatttaaaga taaagaatct ggagaaaaag caatcaagca actatccaat
      541 cttaagaata caaaagtttt atatacttat gatagaattt ttaacggtag tgccatagaa
      601 acaactccag ataacttgga caaaattaaa caaatagaag gtattacatc ggttgaaagg
      661 gcacaaaaag tccaacccat gatgaatcat gccagaaagg aaattggagt tgaggaagct
      721 attgattacc taaagtctat caatgctccg tttgggaaaa attttgatgg tagaggtatg
      781 gtcatttcaa atatcgatac tggaacagat tatagacata aggctatgag aatcgatgat
      841 gatgccaaag cctcaatgag atttaaaaaa gaagacttaa aaggaactga taaaaattat
      901 tggttgagtg ataaaatccc tcatgcgttc aattattata atggtggcaa aatcactgta
      961 gaaaaatatg atgatggaag ggattatttt gacccacatg ggatgcatat tgcagggatt
     1021 cttgctggaa atgatactga acaagatatc aaaaacttta acggcataga tggaattgca
     1081 cctaatgcac aaattttctc ttacaaaatg tattctgacg caggatctgg gtttgcgggt
     1141 gatgaaacaa tgtttcatgc tattgaagat tctatcaaac acaacgttga tgttgtttcg
     1201 gtatcatctg gttttacagg aacaggtctt gtaggtgaga aatattggca agctattcgg
     1261 gcattaagaa aagcaggcat tccaatggtt gtcgctacgg gtaactatgc gacttctgct
     1321 tcaagttctt catgggattt agtagcaaat aatcatctga aaatgaccga cactggaaat
     1381 gtaacacgaa ctgcagcaca tgaggatgcg atagcggtcg cttctgctaa aaatcaaaca
     1441 gttgagtttg ataaagttaa cataggtgga gaaagtttta aatacagaaa tataggggcc
     1501 tttttcgata agaataaaat cacaacaaat gaagatggaa caaaagctcc tagtaaatta
     1561 aaatttgtat atataggcaa ggggcaagac caagatttga taggtttgga tcttaggggc
     1621 aaaattgcag taatggatag aatttataca aaggatttaa aaaatgcttt taaaaaagct
     1681 atggataagg gtgcacgcgc cattatggtt gtaaatactg taaattacta caatagagat
     1741 aattggacag agcttccagc tatgggatat gaagcggatg aaggtactaa aagtcaagtg
     1801 ttttcaattt caggagatga tggtgtaaag ctatggaaca tgattaatcc taataaaaaa
     1861 actgaagtca aaagaaataa taaagaagat tttaaagata aattggagca atactatcca
     1921 attgatatgg aaagttttaa ttccaacaaa ccgaatgtag gtgacgaaaa agagattgac
     1981 tttaagtttg cacctgacac agacaaagaa ctctataaag aagatatcat cgttccagca
     2041 ggatctacat cttgggggcc aagaatagat ttacttttaa aacccgatgt ttcagcacct
     2101 ggtaaaaata ttaaatccac gcttaatgtt attaatggca aatcaactta tggctatatg
     2161 tcaggaacta gtatggcgac tccaatcgtg gcagcttcta ctgttttgat tagaccgaaa
     2221 ttaaaggaaa tgcttgaaag acctgtattg aaaaatctta agggagatga caaaatagat
     2281 cttacaagtc ttacaaaaat tgccctacaa aatactgcgc gacctatgat ggatgcaact
     2341 tcttggaaag aaaaaagtca atactttgca tcacctagac aacagggagc aggcctaatt
     2401 aatgtggcca atgctttgag aaatgaagtt gtagcaactt tcaaaaacac ggattctaaa
     2461 ggtttggtaa actcatatgg ttccatttct cttaaagaaa taaaaggtga taaaaaatac
     2521 tttacaatca agcttcacaa tacatcaaac agacctttga cttttaaagt ttcagcatca
     2581 gcgataacta cagattctct aactgacaga ttaaaacttg atgaaacata taaagatgaa
     2641 aaatctccag atggtaagca aattgttcca gaaattcacc cagaaaaagt caaaggagca
     2701 aatatcacat ttgagcatga tactttcact ataggcgcaa attctagctt tgatttgaat
     2761 gcggttataa atgttggaga ggccaaaaac aaaaataaat ttgtagaatc atttattcat
     2821 tttgagtcag tggaagaaat ggaagctcta aactccaacg ggaagaaaat aaacttccaa
     2881 ccttctttgt cgatgcctct aatgggattt gctgggaatt ggaaccacga accaatcctt
     2941 gataaatggg cttgggaaga agggtcaaga tcaaaaacac tgggaggtta tgatgatgat
     3001 ggtaaaccga aaattccagg aaccttaaat aagggaattg gtggagaaca tggtatagat
     3061 aaatttaatc cagcaggagt tatacaaaat agaaaagata aaaatacaac atccctggat
     3121 caaaatccag aattatttgc tttcaataac caagggatca acgctccatc atcaagtggt
     3181 tctaagattg ctaacattta tcctttagat tcaaatggaa atcctcaaga tgctcaactt
     3241 gaaagaggat taacaccttc tccacttgta ttaagaagtg cagaagaagg attgatttca
     3301 atagtaaata caaataaaga gggagaaaat caaagagact taaaagtcat ttcgagagaa
     3361 cactttatta gaggaatttt aaattctaaa agcaatgatg caaagggaat caaatcatct
     3421 aaactaaaag tttggggtga cttgaagtgg gatggactca tctataatcc tagaggtaga
     3481 gaagaaaatg caccagaaag taaggataat caagatcctg ctactaagat aagaggtcaa
     3541 tttgaaccga ttgcagaagg tcaatatttc tataaattta aatatagatt aactaaagat
     3601 tacccatggc aggtttccta tattcctgta aaaattgata acaccgcccc taagattgtt
     3661 tcggttgatt tttcaaatcc tgaaaaaatt aagttgatta caaaggatac ttatcataag
     3721 gtaaaagatc agtataagaa tgaaacgcta ttcgcgagag atcaaaaaga acatcctgaa
     3781 aaatttgacg agattgcgaa cgaagtttgg tatgctggcg ccgctcttgt taatgaagat
     3841 ggagaggttg aaaaaaatct tgaagtaact tacgcaggtg agggtcaagg aagaaataga
     3901 aaacttgata aagacggaaa taccatttat gaaattaaag gtgcgggaga tttaagggga
     3961 aaaatcattg aagtcattgc attagatggt tctagcaatt tcacaaagat tcatagaatt
     4021 aaatttgcta atcaggctga tgaaaagggg atgatttcct attatctagt agatcctgat
     4081 caagattcat ctaaatatca aaagcttggc gagattgcag aatctaaatt taaaaattta
     4141 ggaaatggaa aagagggtag tctaaaaaaa gatacaactg gggtagaaca tcatcatcaa
     4201 gaaaatgaag agtctattaa agaaaaatct agttttacta ttgatagaaa tatttcaaca
     4261 attagagact ttgaaaataa agacttaaag aaactcatta aaaagaaatt tagagaagtt
     4321 gatgatttta caagtgaaac tggtaagaga atagaggaat acgattataa atacgatgat
     4381 aagggaaata ttattgctta tgacgatggt agtgccttac aatatgaaac tgaaaaactt
     4441 gacgaaataa aatcaaaaat ttatggcgtt ctaagcccat ctaaagatgg acactttgaa
     4501 attcttggaa agataagcaa tgtttctaaa aatgccaagg tatattatgg aaatagctat
     4561 aaatcgatag aaatcaaagc gactaagtat gactcacatt caaaaacgat gacatttgat
     4621 ttatacgcta atattaatga tattgtggat ggattagctt ttgctggaga tatgcggttt
     4681 tttgttaaag atgataatcg aataaaagct gaaactaaaa ttagaatgcc tgaaaaaaat
     4741 aaggaaacta aagcagaata tccctatgta tcaagttatg ggaatgtaat cgaattagga
     4801 gaaggagatc tttcaaagaa caaaccagac aatttaacta aaatggaatc tggtaaaatc
     4861 tattctgatt cagaaaaaca acaatatctg ttaaaggata acatcattct aagaaaaggc
     4921 tatgcactaa aagtgactac ctataatcct ggaaaaacgg atatgttaga aggaaatgga
     4981 gtctatagca aggaagatat agcaaaaata caaaaggcca atcctaatct aagagtcctt
     5041 tcagaaacaa caatttatgc tgatagtaga aatgttgaag atggaagaag tacccaagct
     5101 gtattaatgt cggctttgga cggctttaac attataaggt atcaagtgtt tacatttaaa
     5161 atgaacgata aaggggaagc tatcgataaa gacggaaatc ttgtgacaga ttcttctaaa
     5221 cttgtattat ttggtaagga tgataaagaa tacactggag aggataagtc caatgtagaa
     5281 gctataaaag aagatggctc catgttattt attgatacca aaccagtaaa cctttcaatg
     5341 gataagaact actttaatcc atctaaatct aataaaattt atgtacgaaa tccagaattt
     5401 tatttaagag gtaagatttc tgataagggt ggttttaact gggaattgag agttaatgaa
     5461 tcggttgtag ataattattt aatctacgga gatttacaca ttgataacac tagagatttt
     5521 aatattaagc tgaatgttaa agacggtgac atcatggact ggggaatgaa agactataaa
     5581 gcaaacggat ttccagataa ggtaacagat atggatggaa atgtttatct tcaaactggc
     5641 tatagcgatt tgaatgctaa agcagttgga gtccactatc agtttttata tgataatgtt
     5701 aaacccgaag taaacattga tcctaaggga aatactagta tcgaatatgc tgatggaaaa
     5761 tctgtagtct ttaacatcaa tgataaaaga aataatggat tcgatggtga gattcaagaa
     5821 caacatattt atgtaaatgg aaaagaatat acatcatttg atgatattaa acaaataaca
     5881 gacaagacac taaacattaa gattgttgta aaagattttg caagaaatac aaccgtaaaa
     5941 gaattcattt taaataaaga tacgggagag gtaagtgaat taaaacctca tagggtaact
     6001 gtgaccattc aaaatggaaa agaaatgagt tcaacgatag tgtcggaaga agattttatt
     6061 ttacctgttt ataagggtga attagaaaaa ggataccaat ttgatggttg ggaaatttct
     6121 ggtttcgaag gtaaaaaaga cgctggctat gttattaatc tatcaaaaga tacctttata
     6181 aaacctgtat tcaagaaaat agaggagaaa aaggaggaag aaaataaacc tacttttgat
     6241 gtatcgaaaa agaaagataa cccacaagta aaccatagtc aattaaatga aagtcacaga
     6301 aaagaggatt tacaaagaga agatcattca caaaaatctg attcaactaa ggatgttaca
     6361 gctacagttc ttgataaaaa caatatcagt agtaaatcaa ctactaacaa tcctaataag
     6421 ttgccaaaaa ctggaacagc aagcggagcc cagacactat tagctgccgg aataatgttt
     6481 atagtaggaa tttttcttgg attgaagaaa aaaaatcaag attaagacaa aagctataga
     6541 aaaaaatggt ttatgtactg agattagata gtgaggtgat gacatagttt tgtgaaaacg
     6601 gccatttata actcaattat ttagtttact tcacttcact attgatacta tttggagtta
     6661 ttaatggact tagtttagat aactaatgaa ttgattgaaa gggttagtat tgacaatatt
     6721 ggtcatattg attagaaaat agagtctatc aaaatttaaa ggctaataga ggtgatgaga
     6781 caatttcaag gaattaagta gcgtttggtt gcttaattcc tttttatttg gaaggaagtt
     6841 tttcgtaacc gcccaataac gaagtctatt ggacggttac gatatgatca ctgacttcgt
     6901 cagtcttatc tacaacctca aaactgtgtt ttgagcaacc tgcggctagc ttcctagttt
     6961 actctttgat tttcattgag tattagaaca gaaaaaatgc ttggagtatt tgtttgtgtg
     7021 tttattttta tataacaaac tataaacaaa acaaaaatat aaaaaaaaga gacaaaaaag
     7081 aacaaaaagt aattgacaac ggtttcatat agtgttatac ttatggcata aagttaatgg
     7141 aaagaaggga aaaacttatg gtattagatt atttctttga caaaaacctt gtgttttgct
     7201 tagaagcgga taatcaagaa catctctttg atcaggttgc aactttattg gaagaacgag
     7261 aaattgtaac tccaacttat cgtgaagcct tgatcacgcg tgaaaagtca tttccaactg
     7321 gtttagatat ggaatttcta ggaaaggact tgccaaatgt agcgattcct cacacagata
     7381 ttgttcataa tctagctgag aaagtggtgg ttgttcgatt agaaaaacca gtaacttttc
     7441 acaatatgat agctcctgat aaggaagtag aagtatctct actcttcttt atcattaaca
     7501 attcaagttc aagtcaaacg aacattctgg ctcagttgat ggactttttc acaggaaatg
     7561 gtcatcttga agacctatca aaaatttctg aaccagaaaa actttatgct tacattgctg
     7621 aagcaaccgc ttaatcttgt ctattaaaaa ataaaatcgg aggaaatcca aatgattaaa
     7681 attcttgctg cctgcggtgc aggtgttaac tcaagtcacc aaattaaaag tgctctagaa
     7741 gaagaacttt caaaccgtgg ttatgatgtt cactgtgatg cagtcatggt gaaagatgta
     7801 aacgaagacc ttatgaaagg ttacgatatc tttacaccaa tcgctgcaac agaccttggt
     7861 tttgaaccag gtatcccagt tatcgaagct gggccaatct tattccgtat cccagcaatg
     7921 agcgctccag tatttgacaa tattgaagca gctattaaag aacatggatt aagctaattc
     7981 taatttgtta acattcatat aacataaaaa agggaggaac tgttatggat gcaatctttg
     8041 acctaatcgg aaaggttttc aatcccatct tagaaatggg tggacctgtc atcatgttga
     8101 tcattttgac agtattggct ttactttttg gagtgaaatt ctccaaagcg cttgaaggtg
     8161 gtatcaaact tgccatcgct cttacaggta tcggtgctat catcggtatg ctaaatggtg
     8221 ctttctcagc atcacttgca aaattcgttg aaaacactgg tatccaattg agtattacag
     8281 acgttggttg ggcaccactc gcaacaatca cttggggttc tgcttggaca ctatacttct
     8341 tgctcatcat gttgattgtc aacgtagtga tgcttgctat gaagaaaacc gatacacttg
     8401 atgtcgatat ctttgatatc tggcacttgt ctatcacagg tctcttgatt aaatggtatg
     8461 ctgataacaa tggtgtgagt caaggggttt cactctttat tgctacagca gctatcgtcc
     8521 ttgtcggtgt gttgaaaatt atcaactctg acttgatgaa acctacattt gatgaccttc
     8581 ttaacgcccc aagttcatca ccaatgacat caactcacat gaactacatg atgaacccag
     8641 ttatcatggt tttggataag atttttgaaa aattcttccc aggccttgat aaatatgact
     8701 ttgatgctgc taaattgaac aagaaaatcg gtttctgggg atctaaattc ttcatcggtt
     8761 tcatccttgg tatcgttatc ggtattatgg gaactccaca tccaattgca ggtgttgcag
     8821 atgcagataa atggcgtctt gttatcaaag gatggttgtc tcttggtttg actgccggtg
     8881 tatctttgga actcttctca cttatcggtt catggttcat cgcagccgta gaaccactat
     8941 cacaaggtat tacaaacgtt gctactaaac gtcttcgagg acgtaaattc aatatcggtc
     9001 ttgactggcc attcatcgct ggtcgtgctg aaatctgggc ttgtgccaac gtacttgcac
     9061 caatcatgtt gattgaagca gtgcttcttt caaaagttgg aaatggtatc ttgccacttg
     9121 caggtatcat cgctatgggt gttactccag ctctcttggt tgtaactcgt ggtaaattgc
     9181 tccgtatgat tatcttcgga acactcttgt tgccactctt ccttctttca ggtacactta
     9241 ttgcaccatt tgcaacagaa cttgctaaag gtgtaggtgc cttcccagaa ggtgtgagcc
     9301 aaactcaatt gattactcac tctactcttg aaggaccaat cgaaaaactt cttggttgga
     9361 caattggtaa cactacaact ggtgatatca aagcaatcct tggtgcagta gtcttccttg
     9421 tattctatat cggtatcttt gcttggtaca gaaaacaaat gatcaaacgt aacgaagagt
     9481 acgcagcaaa agcaaaataa tgcgctccta taaaatataa actcaatgaa aatcaaagag
     9541 caaactagga aactagccgc aggttgctaa aaacacagtt ttgaggttgt agataagact
     9601 gacgaagtca gatcaaaaca tggttttgag gttgtagatg aaactgacga agtcagtaat
     9661 catatctacg gcaaggcgac gctgacgaag tttgaattgg attttcgcag agtgtaaatt
     9721 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
     9781 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
     9841 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
     9901 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
     9961 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
    10021 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
    10081 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
    10141 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
    10201 gaagatgcta aagctgtaac aaatgaagaa atgaatcaaa tgattgaaga caggaaagtg
    10261 gattttaatc aaaattggca ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
    10321 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
    10381 aatttcgatc atgaatctcc tgcacaaaat gaaggtggac aactcaacgg tggggaagct
    10441 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
    10501 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
    10561 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
    10621 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
    10681 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
    10741 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
    10801 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
    10861 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
    10921 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
    10981 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
    11041 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
    11101 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
    11161 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
    11221 aaggagatgg gagttaactc tatccgtaca acccacaacc ctgctagtga gcaaaccttg
    11281 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac gtggtatggt
    11341 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
    11401 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
    11461 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
    11521 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
    11581 tatgttacca tgggagcaga taaattccgt ttcggcaatg gtagcggagg gcatgagaaa
    11641 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
    11701 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcatcagc aacccgtaca
    11761 cgaggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
    11821 tatgaacagt cagattatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
    11881 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
    11941 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
    12001 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
    12061 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
    12121 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
    12181 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
    12241 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
    12301 cttgaatgga aagttgccta tcaaccaggt accttggaag caattgctcg tgatgaatct
    12361 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
    12421 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
    12481 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
    12541 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
    12601 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
    12661 gtcaaatcaa ctgaacaagc agggaaattc acccttactg ctcactctga tctcttgaaa
    12721 tctagtcaag tcactgtctt tactggtaag aaagaaggac aagaaaagac tgttttgggg
    12781 acagaggtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc taccactgtt
    12841 ccgtttctat acagtgatgg tagtcgtgca gaacgtcctg taacctggtc ttcagtagat
    12901 gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga agtagaagct
    12961 cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat tgctccaaat
    13021 actgacttga attctgtaga caaatctgtt tcctatgttt tgactgatgg aagtgtacaa
    13081 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
    13141 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
    13201 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaagca
    13261 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
    13321 ttgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
    13381 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggccc tcttcaaacc
    13441 tatgcaattc aattccttga agaagcgcca aaaattgctc acttgagttt gcaagtggaa
    13501 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
    13561 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
    13621 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctgaac
    13681 gctgaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
    13741 aagattgcgc aatccatccg tcctgtaaat gtagtgacag atttgcatca ggaaccaagt
    13801 cttccagcaa cagtaacagt tgagtatgac aaaggtttcc ctaaaactca taaagtcact
    13861 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
    13921 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
    13981 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagtgttcgg
    14041 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtcca
    14101 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
    14161 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
    14221 caatggaccg gttcagaatt gccacttgcc tttgcttcag actcaaatcc aagcgaccca
    14281 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
    14341 aactggaatc gtagtaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
    14401 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
    14461 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
    14521 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
    14581 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
    14641 gataaagttg aaacctatgc tattcgtatt cgcatggtta aagcagataa caagcgtgga
    14701 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
    14761 acaagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
    14821 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
    14881 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
    14941 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
    15001 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
    15061 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
    15121 ctgacagttg aatgggaaga agttccagcg gaaaatctga caaaagcagg tcaatttact
    15181 gttcgaggcc gtgtccttgg tagtaacctt gttgctgagg tcactgtacg agtgacagac
    15241 aaacttggtg agactctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
    15301 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
    15361 ggagatcatt cagaaaatcg tcgttggaca aactggtcac caacaccatc ttctaatcca
    15421 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgca
    15481 caaggaaaag ttcagttctt tgcagatagt ggtacggatg caccatctaa actcgtttta
    15541 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
    15601 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcatta tcgtgcggat
    15661 aaagacattg aagccggtga tgaaattaac gttaccttta aagctgtcaa agccaaagcc
    15721 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
    15781 ttcctggcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
    15841 aaagagcttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
    15901 cggccaaaag tctcagttga agaaaataat caagtagctt caactgtggt agatagtgga
    15961 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
    16021 taccgtatcc acttgactaa ggaaaaacca gtttctgaga agatagttgc tgctgtacaa
    16081 gaaaatcttc caaaactcga atttgttgaa aaagatttgg cctacaagac agttgagaaa
    16141 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa agttggaaaa
    16201 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
    16261 gtagaagttc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
    16321 aaaaaaccac aagtgtccga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
    16381 caaactaata aagcccagtt accaaataca ggtagtgcgg caagccaagc agcagtagca
    16441 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
    16501 gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc ttgtcctaaa
    16561 tagaagataa agagtctggg acaaaatagt tctcaaccac aaaaaagcta gatatttcca
    16621 attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt agattgaaac
    16681 tagagtagta cacttctact tctaaaacat tgttagaaat cgatttgact gtccttatcg
    16741 atttgtcctg ttcttatttc atttcactat gttttaggag gttattggcc ataagtacca
    16801 atcccatgtc aattctcact tgacgcttgc ctctcagatt acatctcttg taacccaaac
    16861 aagcctttat ctgcccaaag acaggttcca catcaatctt gcgttgagcg aaaatctgtc
    16921 tatcttgggg agataaaagc gcctgacatt ctttagcttt caagttttga tagcgttcgt
    16981 tcatatacag tcccttttga ggggctgatt caggttcatc agcgtagtaa accttgattt
    17041 cctgttgaaa gtctgtctgt gttggtactt gatatggtga aaacgatagc atcagccatc
    17101 aggatgtatg tagctatcct ccttgtcatt atagtgccaa ttcgctaagt ttttagctga
    17161 ctctttatac cctctctttt gtttcttatc aaacatggca tatttaatca gaaggggagt
    17221 gatttcttct tgaaaataga cttgtatctg ttcttgaagt ttggcggaga atttctctgt
    17281 cgttttcttc cacacaaaac tatacttgtt ggcattggct tcaatcttag ttccgtcaat
    17341 aaacaaacaa tctaaggtca ctaactcttc catttttcaa tttttcgacc agagaaaatc
    17401 ccttgtgaat aggcaaatag aagagtagat acaagcattt tagggtgata agacgggcga
    17461 tcaaaggcat gataggaggt gtagaagtga cgttcctcca aggtattcac cactttttca
    17521 atagtaaaga cgagatggtc ttgtggtaag aaggaactga tttctagtgg taaagttgtt
    17581 tgatttgtgt tatagtgaat atgcatggga tagccttttt aaatggttta ttaagcaatt
    17641 ctattttacc aagactatcg taaccatgca aaaaaagcaa cggcaaatcc gttgcttttt
    17701 ttggatataa gagactattg tcccagactc ttttctttgt ataaaattgt atagtgttat
    17761 tgtctatact attttgtaga gaaagcaaaa atccctagaa aatgcttagg gattcgacta
    17821 tttcattaag aacaattcaa caatcatttc tatatacatg ataagggtta ataagaatcc
    17881 tgcacgccag aagaatttga tgaatttagg atagtaaaag ctgcgttttt tcaaaaggaa
    17941 gaaaaagacg agaataatgg ctaggaacga gagggctagc cccagtctag ggaggaaatt
    18001 atgggtaaag gttttagctg taatcaagta atactcaaat accaagactg gaaaagccaa
    18061 atccgcaaag tttcgtccta gtttttttaa tctaaaaagt ttggttagga taatgcagat
    18121 gactaaggtc agtatcaata ataaaataga tgctaatttc attaaaatca tacccatatt
    18181 gtatcataaa aaaaggctaa tggaaacgag aaacagagga atttatagga aagtcaggct
    18241 tttgaaattg tgggtgattt ttgttataat gaaagttata aaatcttata ataccttgaa
    18301 tgattattat cgaaaactct ttggagaaaa gacttttaaa gtccctattg atgcgggatt
    18361 tgactgtccc aatcgtgatg gaactgtggc tcatggaggc tgtacttttt gtacggtttc
    18421 cggttctgga gatgccattg tggcaccaga tgcgcctatc cgtgagcaat tttataagga
    18481 aattgacttt attcatcgta agtggccaga tgttcagaag tatctggttt attttcaaaa
    18541 ttttaccaac acccatgaaa aggtggaagt tattcgagag cgttatgaac aggctatcaa
    18601 tgagccaggt gtggtaggaa tcaatattgg aacgcgccca gactgtttac cagacgaaac
    18661 catcgaatat ttggctgagt tatcggagtg catgcatgtg acggttgaat tgggcttgca
    18721 aactacttat gaagcaacct ctgacctgat taaccgtgcg cattcttatg aattgtaggt
    18781 ggaaacggtc aagcgtttga gaaagtatcc aaaaattgag attgtttccc atttgatcaa
    18841 tggtttgcct ggtgaaaccc atgagatgat ggttgaaaat gtccgccgtt gtgtcacgga
    18901 taacgatatt caagggatta aactgcattt gctccatctt atgacaaata ctcgtatgca
    18961 acgagattac catgaggggc gtttacaatt gatgagtcag gacgaatatg tcagggtcat
    19021 ctgtgaccaa ctggaaatta ttcccaaaca tatcgtcatc catcgaatta caggagatgc
    19081 gcctagggat atgctgcttg gtcctatgtg gagcctcaat aaatgggaag tcctcaacag
    19141 cattgagatg gagatgcgac gtcgtggaag tgttcaagga tgcaaggctg taaaacagga
    19201 gtttgaaaat gaaaagacca cttgagatgg cacatgattt tttggctgag gtcgtgacaa
    19261 aagaggatgt cgtagtggat gcgactatgg gaaatggtca tgacacgctt tttttagcca
    19321 agctagccaa gcaagtctat gcctttgata ttcagaagca agccttggaa aagacccaag
    19381 agcgtttgca tcaggctgac ttgacaaatg cccagttaat cttgcaaggc catgagacac
    19441 tggaccagtt tgtgataaaa gctaaggcag ggatttttaa tctgggctat ttgccggcag
    19501 ctgataagtc tgtcatcacc cgaccgcaga caacgattga ggcattagaa aagctatgtg
    19561 gcttacttgt caaaggtgga cgaattgcta ttatgattta ctatggtcat gaaggaggcg
    19621 atctcgagag agatgctgtc ttggattttg tgatccagtt gaaccaacaa gagtacacag
    19681 ctgccattta ccgaacttta aaccaagtca acaacccgcc gtttttagtg atgattgaaa
    19741 aattagagag atacagacat ggataaacaa tacttacacg aaaagctgga tgccatgcgc
    19801 cagaattttg ttgaatcaac ccaccacgaa cgagcgatgg gcgtgctaga ccaagcgcat
    19861 atgagcaaaa aaatgcttaa aatcaagaaa aaattagttg ctcttgaaat ggaacggtgc
    19921 cagagaaaaa ttgagcacaa agactgttcc aagattgacc aaaaaatcaa agagcagaag
    19981 gagatatttg aatcctgttg taaaaaagat taaggaggac gtgcgtggaa ttactgattt
    20041 acctcatcct atttttactg gttttgattg tctcaagtac aaccaataag ctcctgccct
    20101 ttttgcctct ccctttggtg caaattcttt tgggaattgt gattggtctc tttttaccca
    20161 atactgactt tcaccttaat acggagttgt ttttggcact ggttatcgga cccttgcttt
    20221 tccgagaggc tgaagaagca gatgttacgg ctattttaaa acactggcga atcattgttt
    20281 atctcatatt tccagtgatt tttatctcga ccctgagttt gggtggcttg gcccatcttc
    20341 tttggttcag ccttcccttg gcagcttgct tggctgttgg ggcagccctt ggtcctacgg
    20401 acttggtggc ctttgcctct ctttcggagc gttttagctt tcctaagcgc gtgtccaata
    20461 ttcttaaggg cgaaggactc ttgaatgatg cttctggttt ggtggctttt caggtagctt
    20521 tgacagcttg gacaactgga gctttttctc tggggcaagc tagcagttcg ctcatctttt
    20581 caatcctagg cggtttttta attggatttt taacagccat gaccaaccgc ttcctccata
    20641 ccttcttgct aagtgtgcgc gcaacggata ttgccagtga acttttatta gaattgagtt
    20701 tgcctctagt gaccttcttt ctggcagaag aagttcatgt ttcaggtatt attgccgtcg
    20761 tagttgctgg aattttaaag gcaagtcgct tcaagaaaat cacgctcctc gaagcccaag
    20821 tggatacggt gaccgagacg gtctggcata cagtgacctt tatgctcaac ggttctgtct
    20881 ttgtgatttt agggatggag ttggaaatga tagcagaacc tatcttgacc aatccaatct
    20941 ataatcctct acttttattg ctatctctta tcgcccttac ctttgtcctt tttgtcattc
    21001 gttttattat gatctatggc tattatgcct atagaacccg acgcctcaag aaaaagctaa
    21061 ataagtatat gaaggacatg tttctcttga ccttttcagg tgttaaggga acggtgtcga
    21121 ttgctacgat tctcttgata ccaagtaatc tagaacagga gtatcctctt ttgcttttcc
    21181 ttgttgcagg tgtgacgctt gttagctttt taacaggtct cttggtcttg cctcatcttt
    21241 ctgatgaaga ggaagaaagc aaggattatc ttatgcatat cgccattttg aatgaagtaa
    21301 cgctagagtt ggaaaaagag ttggaagaca ccagaaataa acttcccctc tatgcggcta
    21361 ttgacaatta tcatggacgt attgaaaatc tcattttaag ccaagaaaac caggatgatc
    21421 aagaagactg ggctgctttg aaactcttga ttcttagtat tgaaagtgat ggtttggaac
    21481 aggcctatga agaggggaac attagcaatc gtgcttaccg agtttaccaa cgttatctga
    21541 aaaatataga acaaggaatc aatcgtaaac ttgcctcaag actgacctat tattttcttg
    21601 tttccttgag gattttacgt tttctccttc atgaagtttt tactcttgga aagaccttcc
    21661 gtagctggaa ggacaaggag caaagccgtc tccgtgctct tgattatgac caaattgcag
    21721 agctctatct tgccaataca gagatgatta ttgaaagttt ggaaaacctg aagggagtct
    21781 acagacgctc tttgattagt tttatgcagg agtctcgtct tcgagaaaca actattatca
    21841 gcagtggtgc ctttgtcgaa cgggttatca atcgtgtcaa acccaacaat atcaatgaaa
    21901 tgctgagagg ctattatctg gagcgcaagt tgattttcga atacgaagaa aaacgattaa
    21961 ttacaactaa gtatgccaag aaattacgac aaaatgtaaa taacttagag aactattcct
    22021 tgaaggaagc tgccaatacc ctgccgtatg atatggtgga attggtaaga agaaattagt
    22081 taatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtatgtgac
    22141 tgacttcgtc agtcttatct acaacctcaa aacagtgttt tgagcagctt gcggctagct
    22201 ttctagtttg ctctttgatt ttcattgagt ataagattat aaatgaagga gtatgacatg
    22261 aaaaagtggt ggaaagagct gatagacaag cctttattaa aagctttttt gcattattat
    22321 caagcatcag atagtgagtt gaccagtgtc gcagtagcct actattggtt gatttcgatt
    22381 ttccccctgc ttttggtggt ggtcaatatc ctcccctatt ttcagattcc tgtgggagaa
    22441 tttctgggct ttatgaaaga cgtcctgccc ccaagcctct atgaaggtgt ggaaaaaata
    22501 gccagagaag tcttgacgca accttcaaca ggtttattga gtttttctgt tttatcggcg
    22561 ttatggagtt tttcaaaatc tataaatttc ttgcaaaaag cttttaacaa ggcttatggc
    22621 gtcgaaaaga gtcgtggact tatttcccat cagatgctaa gtctcctagt cagttttggc
    22681 ttgcagttcc tctttgcgtt tgccttgttt ttgatcttat ttggtcagat gattttggct
    22741 ttacttgctc attattggac aaaagatggc attatctatc agactttaca agggttggca
    22801 ggtcctctga tttacgccct cctctttgct atcttggtca tgctttatta ttttcttccc
    22861 aatctatcta acaggaaaat tagctatacc ctaccaggca gtgcctttgt tctcttggtg
    22921 attttaggtg tactaactct gttctctagt tatctcaatt actatgtcca tcatttggtg
    22981 gatgtccgaa ttttaggttc cgttcttctt gttgttatga tgttttggtt tatcttgatt
    23041 gctaaaattg ttatcctagg cgcggttatc aatgccagta tgcagagttt gaaagatccg
    23101 gtctttaaaa aagactaact atttatccat tataaaagcg catagtatca agactttata
    23161 gaaccttgat actatgcgct tttgtgcttt gacagttttt tgtatagttt ttgtccaaga
    23221 tttctaaatc atctgattaa attcctattt tcccctatca ttgtccctgt tttttcgaaa
    23281 atggaaggag tataataaat ctatcaataa aatgatagga ggaactttat gggtcatatt
    23341 ttcttttttc taagtgtctt tttggcaggg attctatcct tcttttctcc ttgtatctta
    23401 cctttgttac cggtctatac aggagtgtta ctagatgata aggatggtgc tcaggcttct
    23461 agcggcaaat tttcaatctc agttactagt ttattacgaa ctctggcctt tatagcagga
    23521 atttccttta tatttatttt gttgggctat ggagctggtt ttttaggcga tttgctttat
    23581 gcttcttggt tccaatatct tactggggca attattatct ttcttggttt gcaccaaatg
    23641 gagattctac actttaaggg gctttataaa gaaaagaggc tacaactgca aggacagggg
    23701 caaaatggta agggctatag tcaggcattt ttattgggct tgacctttag ttttgcttgg
    23761 acgccttgcg tggggccggt tctggggtct gttttggcct tggcggcttc aggtggttca
    23821 ggagcttggc agggagctgg tctcatgttg gtgtatacgc tgggcttggc gctaccattc
    23881 ttgcttctag ctctgacctc tagttatgtt ttgaaacatt tccgaaaact tcatccctat
    23941 ctcggaatcc tcaaaaaagt gggtggtttt ctcattattg tgatgggact cttggttctg
    24001 tttggaaatg cttcaatttt aagtcaatta tttgaataaa atggaaagga atatcaatat
    24061 gaaaaaatgg caaacatgtg ttcttggagc aggttcgctc ctttgtttga cggcttgttc
    24121 aggcaagtcc gtgactagtg aacaccaaac gaaagatgaa atgaagacgg agcagacagc
    24181 tagtaaaaca agcgcagcta aagggaaaga ggtggctgat tttgaattga tgggagtaga
    24241 tggcaagacc taccgtttat ctgattacaa gggcaagaaa gtctatctca aattctgggc
    24301 ttcttggtgt tccatctgtc tggctagtct tccagatacg gatgagattg ctaaagaagc
    24361 tggtgatgac tatgtggtct tgacagtagt gtcaccagga cataagggag agcaatctga
    24421 agcggacttt aagaattggt ataagggatt ggattataaa aatctcccag tcctagttga
    24481 cccatcaggc aaacttttgg aaacttatgg tgtccgttct tacccaaccc aagcctttat
    24541 agacaaagaa ggcaagctgg tcaaaacaca tccaggattc atggaaaaag atgcaatttt
    24601 gcaaactttg aaggaattag cctaggaggt gtcttatgaa tgataagtta aaaatcttct
    24661 tgttgctagg agtatttttt ctagccataa ccggtttcta tgttctattg atacgaaatg
    24721 cagggcagac agatgcctcg caaattgaaa aggcggcagt tagccaagga ggaaaagcag
    24781 tgaaaaaaac agaaattagt aaagacgcag acttgcacga aatttatcta gctggaggtt
    24841 gtttctgggg agtggaggaa tatttctcac gcgttcccgg ggtgacggat gccgtttcag
    24901 gctatgcaaa tggtagagga gaaacaacca agtacgaatt gattaaccaa acaggtcatg
    24961 cagaaaccgt ccatgtcacc tatgatgcca agcaaatttc tctcaaggaa atcctgcttc
    25021 actatttccg cattatcaat ccaaccagca aaaataaaca aggaaatgat gtggggaccc
    25081 agtaccgtac tggtgtttat tacacagatg acaaggattt ggaagtgatt aaccaagtct
    25141 ttgatgaggt ggctaagaaa tacgatcaac ctctagcagt tgaaaaggaa aacttgaaga
    25201 attttgtggt ggctgaggat taccatcaag actatctcaa gaaaaatcca aatggctact
    25261 gccatatcaa tgttaatcag gcggcctatc ctgtcattga tgccagcaaa tatccaaaac
    25321 caagtgatga ggaattgaaa aagaccctgt cacctgagga gtatgcagtt acccaggaaa
    25381 atcaaacaga acgagctttc tcaaaccgtt actgggataa atttgaatcc ggtatctatg
    25441 tggatatagc aactggggaa cctctctttt catcaaaaga caaatttgag tctggttgtg
    25501 gctggcctag ttttacccaa cccatcagtc cagatgttgt cacctacaag gaagataagt
    25561 cctacaatat gacgcgtatg gaagtgcgga gccgagtagg agattctcac cttgggcatg
    25621 tctttacgga tggtccacag gacaagggcg gcttacgtta ctgtatcaat agcctctcta
    25681 tccgctttat tcccaaagac caaatggaag aaaaaggcta cgcttattta ctagattatg
    25741 ttgattaata ctcttcgaaa gtctcttcaa accacgtcag cgtcgcctta ccgtatgtag
    25801 ggttactgac tccgtcagtt ttatctacaa cctcaaagct atactttgag ctgacttcgt
    25861 cagttttatc tacaacctca aagcggtact ttgagaaacc tgtggctatc ttcctagttt
    25921 actttttgat ttttattgag tataagaggt ctttcctaag cagttagagg agaattttgc
    25981 tatactgata ctagtaagtg gtaaaggagc agagcatgac ctacacaatc ttaatcgtag
    26041 aagatgaata tctggtaaga caaggtttga ctaaactggt caatgtagca gcctacgata
    26101 tggaaatcat cggtcaggct gaaaatggaa ggcaggcttg ggaattgatc caaaagcagg
    26161 tgccagatat cattttaacc gatatcaaca tgcctcatct aaatggcatc cagttggcca
    26221 gtctggtacg agaaacctat cctcaggttc atttggtctt tttaacaggt tacgatgatt
    26281 ttgattatgc cttgtctgct gtcaaactag gtgtggacga ctacctgctc aaaccctttt
    26341 ctcgtcagga tattgaggaa atgttgggga aaatcaaaca aaaactagac aaggaagaga
    26401 aagaagagca gttacaagat ttattgacta ataggtttga aggaaacatg gcccagaaaa
    26461 tccagtctca tctggctgat agccaattta gtttaaagtc tttagccagt gacttaggtt
    26521 ttagtccgac ctatctgagt tccttgatta agaaagagtt gggcttgcct tttcaggatt
    26581 atctggtgag agaacgtgtt aaacaagcta agctcttgct tttaactaca gatctgaaga
    26641 tttatgagat cgcagagaag gttggttttg aagatatgaa ctattttacc caacgtttta
    26701 agcagattgc aggtgtgaca cctcgtcagt ttaagaaggg agaagaccga tgaagcgttc
    26761 ttctctttta gttagaatgg ttatttccat ctttctggtc tttctcattc tcctagctct
    26821 ggttggaact ttctactatc aatcaagttc ttcagccatt gaggccacca ttgagggcaa
    26881 cagccaaacg accatcagcc agactagcca ctttattcag tcttatatca aaaaactaga
    26941 aaccacctcg actggtttga cccagcagac ggatgttctg gcctatgctg agaatcccag
    27001 tcaagacaag gtcgagggaa tccgagattt gtttttgacc atcttgaagt cagataagga
    27061 cttgaaaact gttgtgctgg tgaccaaatc tggtcaggtc atttctacag atgacagtgt
    27121 gcagatgaaa acttcctctg atatgatggc tgaggattgg taccaaaagg ccattcatca
    27181 gggagctatg cctgttttga ctccagctcg taaatcagat agtcagtggg tcatttctgt
    27241 cactcaagaa cttgttgatg caaagggagc caatcttggt gtgcttcgtt tggatatttc
    27301 ttatgaaact ctggaagcct atctcaatca actccagttg gggcagcagg gctttgcctt
    27361 cattatcaat gaaaaccatg aatttgtcta ccatcctcaa cacacagttt atagttcgtc
    27421 tagcaaaatg gaggctatga aaccctacat cgatacaggt cagggttata ctcctggtca
    27481 caaatcctac gtcagtcaag agaagattgc aggaactgat tggacggtgc ttggcgtgtc
    27541 atcattggaa aagttagacc aggttcggag tcagctcttg tggaccttgc ttggggccag
    27601 tgtcacatct cttcttgtct gtctctgctt agtgtggttc agtcttaaac gctggattgc
    27661 tcctttgaag gatttgagag aaaccatgtt ggaaattgct tctggtgctc aaaatcttcg
    27721 tgccaaggaa gttggtgcct atgaactgag agaagtaact cgccaattta atgctatgtt
    27781 ggatcagatt gatcagttga tggtagctat tcgtagccag gaagaaacga cccgtcagta
    27841 ccaacttcaa gccctttcga gccagattaa tccacatttc ctctataaca ctttggacac
    27901 catcatctgg atggctgaat ttcatgatag tcagcgagtg gtgcaggtga ccaagtcctt
    27961 ggcaacctat ttccgcttgg cgctcaatca aggcaaggac ttgatttgtc tctctgacga
    28021 aatcaatcat gtccgccagt atctctttat ccagaaacaa cgctatggag ataagctgga
    28081 atacgaaatt aatgaaaatg ttgcctttga taatttagtc ttacccaagc tggtcctaca
    28141 accccttgta gaaaatgctc tttaccatgg cattaaggaa aaggaaggtc agggccatat
    28201 taaactttct gtccagaaac aggattcggg attggtcatc cgtattgagg atgatggcgt
    28261 tggcttccaa gatgctggtg atagtagtca aagtcaactc aaacgtgggg gagttggtct
    28321 tcaaaatgtc gatcaacggc tcaaacttca ttttggagcc aattaccata tgaagattga
    28381 ttctagaccc caaaaaggga cgaaagttga aatatatata aatagaatag aaactagcta
    28441 actcccagtc aattctggga gttttatgcg taattgggca aactttctag gttgtctatc
    28501 ttgctaaaaa caagaaaaaa cggtatgata gaagagtgac aacagaggta acagagatga
    28561 acgagacaca gattcaaaga gaaacacgtc aggtagtgga agatgtttta gaaaagacca
    28621 atttgaagca gggggccctt tttgttttag gtctatcttc tagtgaggtg ctaggtggtc
    28681 atattgggaa agaatccagt caagagattg gtgagctgat agtggagact atcttggata
    28741 ttctaggtag tagaggtatc catttagcag ttcaaggttg tgagcatgtc aatcgggctc
    28801 tcgttgttga acgtcaggtg gcagagcagt ttggtctgga aattgtcagt gtccatccta
    28861 ctcttcatgc aggaggttcg gggcagttgg cagcctttaa gtttatgcag gatccagttg
    28921 aggttgaatt tatcaaggct catgctggat tggatatcgg agacactgca attggcatgc
    28981 atgtcaagca tgttcaggtt ccgattcgcc ctattttgag agagattggt catgcccatg
    29041 taacggcact ggctagtcgt ccaaaattaa tcggaggtgc gcgtgcgcac tatccgcaag
    29101 atgctattag aaagacctga gaatgaataa aggagataaa agtgtttaaa aaagaccgtt
    29161 tttcaattcg taagattaag ggagttgtag gctctgtatt tcttggaagc cttttgatgg
    29221 ctccttctgt agtggacgca gccacctatc actatgtaaa taaagagatt atttcacaag
    29281 aagctaaaga tttaattcag acaggaaagc ctgacaggaa tgaagttgta tatggtttgg
    29341 tgtatcaaaa agatcagttg cctcaaacag ggacagaagc atctgttttg acagcttttg
    29401 gttatctatc aggtgacatc ctcaaaacgc ttggcttgga cactgtttta gaagaaacct
    29461 cagcaaaacc tggagaggtg actgtagtcg aagttgagac tcctcaatca acaacaaatc
    29521 aggagcaagc taggacagaa aaccaagtag tagagacaga ggaagctcca aaagaagaag
    29581 cacctaaaac agaagaaagt ccaaaggaag aaccaaaatc ggagataaaa cctactgacg
    29641 acacccttcc taaagtagaa gaggggaaag aagattcagc agaaccagct ccagttgaag
    29701 aagtaggtgg agaagttgag tcaaaaccag aggaaaaagt agcagttaag ccagaaagtc
    29761 aaccatcaga caaaccaaca gaggaaccaa aagttgaaca agtaggtgaa ccagtcgagc
    29821 caagtgaaga cgaacaggca ccaacggcac cagttgagcc agaaaagcaa ccagaagctc
    29881 ctgaagaaga gaaggctgta gaggaaacac cgaaaccaga agataaaata aagggtattg
    29941 gtactaaaga accagttgat aaaagtgagt taaataatca aattgataaa gctagttcag
    30001 tttctcctac tgattattct acagcaagtt acaatgatct tggacctgtt ttagaaactg
    30061 caaaaggtgt ctatgcttca gagcctgtaa aacagcctga ggtaaatagt gagacaaata
    30121 aacttaaaac ggctattgac gctctaaacg ttgataaaac tgaattaaac aatacgattg
    30181 cagatgcaaa aacaaaggta aaagaacatt acagtgatag aagttggcaa aacctccaaa
    30241 ctggggttac agaagctgaa aaagttgcag ctaatacaga tgctaaacaa agtgaagtaa
    30301 attctgagac tgcaagtctt aaaacagcaa tttctagatt aaatacagat aaagttgaac
    30361 tcgaaaatca acttaagatt gcacagggaa aaactgaaac tgatttcagc atggaatctt
    30421 ggactgtttt gagtacagct aaaaataaag ctcaggaagt aaaagataac ggaacagcga
    30481 cccaagaaca aataaacgaa gcagagaaaa gtttgaaaac agcccttgct gacttatctg
    30541 ttgacaaaac agctttggga agcgctatcg atacagctac taagaaaaat aaagagaatt
    30601 atacaaatca aacatgggca gagttggaaa cagcgcttac agcagcaaaa tctgtaaata
    30661 ctaacgagtc taaacaaagc gacgttaacg aagctgctga aaaattaact gcaactatgg
    30721 aaaaattggt tgaattgtct gaaaagccac gtttgactct atctattgaa aagagagaca
    30781 tagatcgaaa agctacggtc acgtatactt tggaaaatcc agcaaatact caaataaaat
    30841 caataactgc aactctgaaa aaaggagagg aagttgttaa ggattttgtt ttaacagaag
    30901 aaaatcttaa aactaatcat ttaactgctt tgtttgaaaa gctagactac tacaaagaat
    30961 acactctatc tacggatatg gtatataaca gaggaaatga tgatgagacg gagagtattt
    31021 cagaagaact aattcagctg aatcttaaga aactagaatt gaaagatatc cagacagtct
    31081 ctctgatgaa atttgaaaat ggtcaagaaa gtcaggttac acacttgtct gataaaccga
    31141 ccgatttgag taaattatac ttaaaagtta catcatcaac gtctaaggat gctgtgttag
    31201 ccgttagctc tatagaagaa gagattattg aaaataaaaa aatctttaaa attcatgcag
    31261 atacaccaga attagtagtt cgtaagaaag atggcagtct ttctaaggga tttgactatt
    31321 acatggagag agttatccct catgatggtg atatttatta tgattttaaa gacttgattt
    31381 cagctatgac atctaatcct acaggtacgt ttattttagg gagagatatt tcctcgcgta
    31441 atgtaaaacc ggatggaaaa tcttatatca aaggtgaatt taaaggaaaa ctacttggta
    31501 caaatgataa tgttagacac tcgatctttg acttagaata tccattgttt gatactataa
    31561 aatcaggagt cgtgaaagat attgatttca agcatgtcaa tatggtattc ccagatagca
    31621 atcaaggaga taacgtagca acaattgcaa gagtaatcaa agataagaca aaaattgaga
    31681 atgttaatgt tgaaggttat ctcgagggtc gagatcatgt agctggttta gttaataatc
    31741 ttgaaggtaa ttccgaaatt gaaaatgtat cctttacagg aaagattaag tctaaaggtg
    31801 gtaattctat tacagcaggt attgcaggta gaaatatctt atctcgtgtt aaacgtgcct
    31861 atgtaaatgc agatatagag gtacatcgat catctaattc tagcatgctt gttgcagtaa
    31921 atggaataaa cgcagatgca agtggtggtt ggggaacctg gggtagactt actgagtcag
    31981 ttgctaaagg aacattagaa accaagcaag gagggcaagc tggaggagcc tcttctacag
    32041 tctggcctta tggagcaatt gataatgttg tcagctatgc aaaagtgacc aaagggaaag
    32101 aattatttgg atcagatggt gatttaaatt atgattggtt tatgaaaaaa attagtaata
    32161 tttttggtgt tcaaggcatc agctcaggcg attcgggtag tgattctaaa tttacgcgta
    32221 tttctgagga agaagcaaat caaaaagttg cttcttataa tattacagct cccaacttga
    32281 tgagtgactc aagtcttttg gtagacagac tcaatgaaag ttggaaaaat actgatcaat
    32341 ttgaaagtat acaggattat caagcccaaa accaattaat ttaccagaac ttgactaaat
    32401 ttactcctta ttacaataaa gaatttattg ttcatgaagg aaatgctctc actcctgaac
    32461 aggaaatatt gaagactaag aaaatcaaat cgattgtagg gttgaaagga acagagttcg
    32521 ttgtagatgg cagtgatatt gatactatca tgcttcattt tgaggatggt agtcagaagc
    32581 gatataaggt aacctctact ggtaagtttt cgataactaa tttaccggaa tatcaggttg
    32641 aagatttaaa tgtggtgtat acatcagaac atatagtaca tccactagat tcgtcactga
    32701 taaataacct tgttgaagaa ttgaaaaaag tagaattgta tacagagtct acttaccaag
    32761 ttctaggtat tgataaagat aatgctaaca aattgaatag aactaagaga ttattcttag
    32821 atgagtcatt agatgctgtt aaaacacaat tgcctacctt tgtaaaaaca atgtttgaaa
    32881 atgagtggtt gcacatcaat ggagaatcta gtggagctgt cgctgcttta cgacaaaaaa
    32941 tcatggataa taagacggct atattgcttg ctttgactta tatcaatcgt tattatgatg
    33001 ttaaattctc agactataat attaaaaaac tgatgctctt taaaccaact ttccatggtg
    33061 aaaagattga tctattagat cgattaatcc gactaggatc atctggagaa aatagattaa
    33121 aaggttcaga aaatgcagaa acgttcaagc agttatttgc tagcgaaacg aagcagaagg
    33181 atttggtgac ttatttagac tacaatcgct ctttgttgac taattatcaa acaacagggg
    33241 aatggtttaa agaaacgact aaggattata ttcaatttga ggaacgtcca tccttagtag
    33301 aggagattaa agatgccaaa tatcgagttt acgataattt aaccgcaccg tattatcaag
    33361 gatatatcct acctctctta acgttgaaga atactcattt agcaatttta tcgaactata
    33421 gtactatgac atttgtcagc cgtgaaaaac gccctaattg gaaaaatgaa gattttgata
    33481 agtgggtgaa atatgtagca actgctcata gaaaccatgt tgatacttgg tataagattt
    33541 tacctgataa tatcaaaggt aaaatggtta aagagaatgt tactgctgtt tgggaaggat
    33601 tgagcatccc aggaagtgaa tgggtagacc agaatgctgt agatagaaaa ggaagagatt
    33661 acgccccagc tcgagaattc tttaatcttg taggtggtcc tatgggaggt tggtatgctt
    33721 accatggtta tggtgcgcat gctggaggaa gaaatcgggt taattatgag gtgtttgatg
    33781 tattaagtga gtatggtata tcggtcttta ctcatgaatt gactcacgtt aatgatactt
    33841 ggatttactt aggagggtat ggtagacgtg aaaatatggg acctgaagct tatgctcagg
    33901 gattattcca gtctcctgtt ccaggtcagc cgggttgggg ggcgcttggt ctcaatatgg
    33961 catttgaaag aaaaaatgat ggtgatctaa tttataatgc ctccccaact caatttgaaa
    34021 ataggaaaga gttagatagt tatatgaaaa attataacga tactctcatg atggtggact
    34081 acttggaagg agatgcagtt atctctaaag gtaaagaagc tattactaaa tggtttaaaa
    34141 aagttgaacc taaggttgtt agtcaaacag ctcagtatga tacggttcgt cagttgactg
    34201 ctgaagagaa agaaaaactt tctgtttcta gtgttgatga tttagttgat caaggattaa
    34261 tgtcagacag agctgtagga aataatacct ataatccagc tgattttgaa acaagttata
    34321 ttgcgataga ttatatgact ggtatttatg gtggaggaaa gaatagtgta ggttcaccag
    34381 gtgccttgat gtttaaacat aatactttcc gtatgtgggg ctactatggt tttgaagaag
    34441 gtgttctagg atatgcttct aataaattta agcaagcatc tagagatgaa ggtcatgctg
    34501 gacttagtga taactttatt atttctaaga tttccaaagg ggaattttta actatggaag
    34561 catttaaaaa gggatatttt aagaaagtag tagaagagtt aaaaactaaa ggtattcgtc
    34621 ctgttactat caatcaaaaa acatatagca cctttgaaga attgcaagaa ggatttaagc
    34681 aggctgttga aagagatttg aagaaaaatc agttagatga gagggaaaca agaaacttca
    34741 aattccaagt tttcagacaa cttcttcaac aaacagatag ctttaaaact tctatcttta
    34801 gataatcgaa atctctcatc tgctttgcgg atgagggttt tcttatttta tgctatactt
    34861 aaagtatgca tagaaaaaca gtgattgatt ttaggacttt gggggagaga tataccttta
    34921 cccagcctat taaagagttg aaaacgagaa atgtagaaga agtggcagat ttgctggcac
    34981 aagtggaaag ctaccaagag caaggttatt atgtggtggg gtatgtcagc tacgaggctg
    35041 cacctgcttt tgaggaaaaa ttagcagttc acaaggatcc cctactggga gagtacctgc
    35101 tttattttac tgttcatgat agcgtagaaa cttcccctat tcctctgact tatgaggata
    35161 ttgatttgcc ctcaaattgg caggaagtaa cgtctgcagc agactatgaa aaggccattg
    35221 cccaaattca ccatcattta cggcagggag atacctatca ggtcaactac accgtccaac
    35281 ttaagcaaaa gttaaatgcc aatccttttg ccatctacaa tcgtatggtg gtagagcagg
    35341 aggcgggcta caatgcctat gtggaacatg acgagatggc agtgatttcc atgagcccag
    35401 agctcttttt tgagcaaaat gatcgcgagt tgacaacacg accaatgaag gggacgactc
    35461 agcgtggggt aactgaccaa gaagatcttg aacaggccag ttggttggaa caggatccca
    35521 aaaatcgctc tgaaaatatg atgattgtgg acctcttgcg caatgatatg aaccgtattt
    35581 ctgaagttgg gagcgagcac gtggagcgtc tgtgtcaggt agagcagtat tcaactgttt
    35641 ggcagatgac ttcgaccatc aagagtcagt tgcgagagga tgtggacctt gttgaaatct
    35701 tccactcact ctttccttgt ggttccataa cgggtgcacc gaaaattgcg acaatggaga
    35761 ttatcaagga cttggagcct caaccgcgtg gagtctactg tggaacgatt ggtctcttgc
    35821 ttccaaatgg acgacggatt tttaatgtgg ccattcgtac cattcaactt cacaaaggtc
    35881 aagccattta tggagttggc ggagggatta cttgggatag cacttgggaa tctgaatacc
    35941 gtgaagttca tcaaaaggca gcagttctct atcgtaaaca agctcgcttc caattaatta
    36001 caactggaaa aatcagccaa aaacaactga tgtttgaaga acaacatctg gaaaggctga
    36061 gaaaagctag tcggtatttt gcttatcctt ttgacccaga aattttgaga caaaagattg
    36121 aagcagagtg tcagacttgt gatgctaatc aagattaccg cttgcgaatt tctctcagca
    36181 agtctggaga gatagaattc agtcgtcaaa tcttaacacc tcttagtcca agtttttgtc
    36241 aggccaaact ttgtctgcaa gaagcgaatt tgaatcaatc ctttacctat tttaaaacga
    36301 cttacagacc gcatttgagc atagaggaac aagagaagat ttaccataat ggaaaaggag
    36361 aacttcttga aacctctatc ggaaatttgg ttctgaaaat cgatgggaaa ctctacacac
    36421 cgcctatccg acttggaatc ttgccaggaa tttatcacca gtatttacta gaaacaggac
    36481 aggtagaaga gaaagtcttg accttggcag attttgccca agcagaagct atttacggct
    36541 gtaatgcagt gagaggcttg tatgagttgc aagtgatagg attgtgagtt ttagctatta
    36601 atagttttta atatatatta aaatatattg gtgtttactt ttgatgagat atcatatttg
    36661 tgttataatg taaccgtaaa caatagaaat ttaaaggaga tttttgatga aatcaaaaat
    36721 agttttggga gcttcgctag cgatcgcaac acttagctta gtatctcttg tcgagattga
    36781 aggactctca ccgtttttaa tagagaatgt tagcgcaaat actcattctg caaataaagt
    36841 tattaatcat aaagtaagta tttacttaga aaatgctgat gaaggtaaag gtctaactgt
    36901 aaacttttca acagacagtg ttagtccaaa tttatttgat gagttcgaaa aaaagagtgg
    36961 aattacaata actactatgt tagtgaatgc taagacaggt gaagttgtag aaaaaagatt
    37021 aacaccaagc gtttttttac gttccaatga tttaaccagt gggaccatat cttcgtttat
    37081 ttttagtgaa tatcctgatg gagaatataa atatgttgtg tctaaaggag actttatcga
    37141 tcctaagact cagtttaaac atcagtatag aggagaatct ccagttttta gaatacgtaa
    37201 tcgaaaatat gtggagttgg gaacaactga taaaaaatta gatgaaagac gtgataatag
    37261 cgtttataaa gatggggttg ttgaacacaa ggtaaattta agtttaacaa gttatcaagg
    37321 aggtaatgga gttactgcaa ttttttcaac tgatagtgtt aactctaact tacttaattc
    37381 ttttggtgaa aaggctaaaa aagttttgat taggtctaag ttaattaatg ttaagactgg
    37441 cgaggttatt gatgaaacgt tttctccaaa agtaagttta acctctaaaa ttttgaaaag
    37501 tggttctaca gcagtatttt attttataga tttaactgat ggcgaatata aatatgttgc
    37561 gtatgaaagc cagcaatata ctgatcctca aacaacattg actcatcagt atagaggaga
    37621 atctcctatt tttagtatca aagatggaaa attttctggt ttagtatctg cctcaaaacc
    37681 agacgaaaac ccgaagccaa cgccaaaacc ggatgagaaa ccaaaacctt cagcaccaca
    37741 acaggagaaa acaaaaccta cagttcagtc tggttgggta ggttcatctt attatcaaaa
    37801 tggtaaaaaa gtcactagta agtggatttt tgataaaaaa tataattcat acttctatct
    37861 agatgcatca ggaaattatg ttcaaaacgc ttgggtaggt aactattatc tcaaatcagg
    37921 cggatacatg gctaaaagtg agtggattta tgataagaac tatggttcat actactatct
    37981 aacatcagaa ggcagctacg ctcgtaacac ttggtcaggc aattattatt tgaagtcaaa
    38041 tggtaagatg gctaaaagtg agtgggtata cgatagtaac tacaaatcat attactactt
    38101 gacatcagaa ggaagttatg ctcgtaacac ttgggtaggc aattactatc ttaaatcaaa
    38161 cggtaaaatg gctgttaatg aacgtacacc agatggttat cgtgtagatg gttcaggtaa
    38221 atgggttaaa taagaaacta aaaaatagga taataggtaa tatcagatga ataaaaaaaa
    38281 ttattttcat gtgctttagc attttcaacg attggaacat tgtcaacgat tgataaatca
    38341 ggagttcttc ctttttcagc gcaaacggtg caggctgaca tacaaacgct taccccaatg
    38401 gcaaaatatt atattattct tccgaaggat gcagaaatat ataaaacttg gagaggtact
    38461 gtcaatattc ccattattga tgctacaaaa actactccag aattatctta tttcaaagaa
    38521 gatcatcgca actatatagc taatgagaat aagtctggtg ccaattacat agagtggaag
    38581 ggaacagtag aagagtttaa agaggccatt aagaagttga ctgataaaaa atcaactact
    38641 gcaacaccaa aaaaggatga aaagccaaca ccaaaaccgg atgagaagcc aaaaccaaca
    38701 cctacagttc agtctggttg ggtaggttca tcctattatc aagatggtaa aaaagtcatt
    38761 agtaagtgga tttttgataa aaaatataat tcatacttct atctagatgc atcaggaaat
    38821 tatgttcaaa acgcttgggt aggtaactat tatcttaaat caggcggata catggctaaa
    38881 ggtgaatggg tttatgatgc cacctatcaa gcttggtatt atttgacatc agatggttct
    38941 tatgcttaca gtacatggca aggaaattac tatctaaaat cggatggtaa aatggctgtc
    39001 aatgaatggg ttgatggtgg acgttattat gttggcgctg acggagtttg gaaggaaggt
    39061 caagcaagta cagcttcttc tagtaatgat agcaatagtg aatattctgc tgctttagga
    39121 aaggcaaaaa gttataattc gttattccac atgtcaaaaa aaaacgtatg tatagacaat
    39181 taacttctga ttttgataaa ttttcaaatg atgcagctca atatgccatt gatcattaga
    39241 tgattaacag atactggtag gcgaaaaaat tccttataat tcatttacgc aaacctttgc
    39301 ataagtgaat ggattttgat atactagtac tgtaagcatt ttcaattact acacaatgaa
    39361 aaaggagaat atgatgagtc aaaagattat tgggattgac cttggtggaa cttctatcaa
    39421 atttgcaatc ttaacaacag caggagaaat ccaaggaaaa tggtcaatca agaccaacat
    39481 tttggatgag ggaagtcata tcgttgatga tatgattgag tctattcaac atcgtttgga
    39541 cttgcttgga ttggcagcag cggacttcca aggcattgga atgggatcac caggtgtggt
    39601 tgaccgtgac aaagggactg ttatcggtgc ctacaacttg aactggaaaa cccttcaacc
    39661 aatcaaacaa aagattgaaa aagctttggg cattccattt ttcatcgata atgatgccaa
    39721 cgtagcagct cttggtgagc gctggatggg tgctggagat aaccaaccag acgttgtctt
    39781 tatgacactc ggtactggtg ttggtggcgg tatcgtcgca gaaggcaaat tgcttcacgg
    39841 tgttgctggt gcagcaggtg agcttggtca catcactgtt gactttgacc agccaatctc
    39901 atgtacttgc ggtaagaaag gctgccttga gacagttgct tcagcaacag ggattgtcaa
    39961 cttgactcgt cgctatgccg atgaatacga aggcgatgca gccttgaaac gcttgattga
    40021 taatggagaa gaagtaactg ctaagactgt ctttgatctc gcaaaagaag gagacgacct
    40081 tgctttgatt gtttaccgta acttctcacg ttacttggga atcgcttgtg ctaacattgg
    40141 ttcaatccta aacccatcaa caatcgtcat cggtggtggt gtgtcagctg cgggagaatt
    40201 ccttctacaa ggtgttcaaa aagtttacga tgaaaatagt ttcccacaag tacgcacatc
    40261 tactaaatta gctcttgcaa ctctaggaaa tgacgctgga gttatcggag cagcatcact
    40321 tgtattgcaa taagataatg agagaggaaa atgctaacct agagttagta atgttcctct
    40381 tttatttttt atgctatact agttaggaca atgaatgagg tgagagtaaa tgacaaaagc
    40441 agatacgatt tttaaagaga atattgaatg cattctaaag gaaggtgttt tctcagagca
    40501 agctcgtcct aagtacaagg atgggactgt tgctaattcc aagtatgtaa ctggtgcctt
    40561 ttccgagtac gacttatcta aaggagaatt tcctatcaca accttgcgtc ccattgcaat
    40621 caaatccgcc atcaaagaag ttctctggat ttaccaagac cagtcaaata gcttagaagt
    40681 gcttaatgac aagtacaatg ttcactactg gaatgactgg gaagttggag acacgggaac
    40741 cattggtgag cgctatggtg ccgttgttaa gaaacacgac attatcaata agcttctcaa
    40801 acagttggaa accaatcctt ggaatcgccg caatattatt tcgctctggg attaccaagc
    40861 tttcgaagaa acagatgggc tgctcccgtg cgcctttcag accatgtttg atgtccggcg
    40921 tgttgatggg gaaatctatc tggatgcgac cttgacccag cgctccaatg atatgctggt
    40981 ggcccaccac atcaacgcta tgcagtatgt ggctttacag atgatgattg ccaaacattt
    41041 tggttggaag gttgggaagt tcttctattt catcaacaac ctccatatct atgataatca
    41101 atttgaacaa gctcaggaat tgctccgtcg ggagccgtca aactgccaac cacgcttggt
    41161 tttaaatgtt cctgatggga ctaatttctt tgatatcaaa gcagaagatt ttgagttggt
    41221 ggattatgac cctgttaagc cacagttgaa gtttgaccta gctatttaaa agaatagaaa
    41281 aaagaagttg agaataatcc caacttcttt tgtttcttaa cgtgatacgc ggcgacgagc
    41341 tgctttttta cgattttctt cgatgaaagc tgctttttgc tcttctggtt cgattacttt
    41401 ctttttaaat gcgtatactg cacctgcaac ggcagcgaca gttcctgcga cacctgttac
    41461 aagaccttta gcgaatcctt tagccatgag tcttcctcct ttatattctc aatcagccag
    41521 cctcctcaag aggtcacatt tttctgactg acctttttgt gttataataa tagtaacgaa
    41581 aaaatgggaa tttttcaagg aaaaaagatg aaaacaaaaa taattgtgat tgttggaccg
    41641 actgctgttg gaaagacggc tctagctatt gaagttgcaa agcgctttaa tggcgaagtg
    41701 gttagtggag atagccagca agtctatcga ggacttgata ttgggacggc caaggctagt
    41761 ccagaagagc aggcagctgt tcctcatcat ttaatcgatg ttagagagat aaccgagtct
    41821 tactcggctt ttgattttgt ttcagaagct aagatgacta ttgaggatat tcagagccgt
    41881 ggcaagctag ccattatcgc cggtgggact ggactttata tccagagctt gctagaaggt
    41941 taccacctag gtggggagac tcctcatgag gagattttag cttatcgagc tagtttggag
    42001 ccatattcag atgaggaatt agcccatttg gtggagcaag caggccttga gattccccag
    42061 tttaatcgtc gtcgtgctat gcgtgcctta gaaattgccc attttggtca ggatttggaa
    42121 aatcaagaga tcttgtatga accgctgatt atctgcttgg atgatgaacg tagtcaactt
    42181 tatgagcgta tcaaccaccg agtggatttg atgtttgagg ctgggctttt ggatgaggcc
    42241 aagtggctgt ttgaccattc ccctaatgta caggctgcta aaggaattgg ctataaggaa
    42301 ctctttcctt atttccgtgg agagcagacc tttgaggaag ctcgtgagag tcttaaacag
    42361 gcgacccgtc gttttgccaa gcgtcagctg acctggttcc gtaatcgcat gcaggtcacc
    42421 ttttatcaga ttggagaatc tggtgtacaa gaccgcattt taagccagat agaggagttt
    42481 ttagatgatt gaaacggaga aaaaagagga gcgagtcctg ctgattggtg tggaattgca
    42541 gggtatggac agttttgacc tctccatgga agaattggct agtttagcga aaacggcagg
    42601 ggcagtcgtt gtagatagct acagacaaaa acgtgaaaaa tatgattcca agaccttcgt
    42661 cggctctggt aagttggaag agattgcgct tatggtggat gcagaagaaa tcactactgt
    42721 catcgtcaac aatcgtctga ccccaaggca gaatgtcaat ctagaggaag ttctcggtgt
    42781 taaggtcatt gaccgtatgc agttgatttt ggatatcttt gccatgcggg ctcgaagcca
    42841 tgaagggaag ctccaagtcc acctagccca actcaaatac ctcttgcctc gcttggttgg
    42901 tcaggggatt atgctcagcc gtcaggcagg gggaattggt tcccgtggtc ctggtgaaag
    42961 ccaactggag ctgaaccgtc gtagcgttcg caatcaaatc acggatatcg agcgccagct
    43021 taaggtggtt gagaaaaatc gtgcgactgt cagagaaaaa cgtttggagt ctagcacttt
    43081 taagattggt ttgattggtt atactaatgc tgggaaatca actatcatga acatcttgac
    43141 cagtaagacc cagtatgaag cagatgagct ctttgcgact ctggatgcga caaccaagag
    43201 tattcatctg ggaggcaatc tccaagtaac tttgacagat accgttggct ttatccaaga
    43261 tttgccgaca gagttggtgt ccagtttcaa gtcaaccttg gaagaaagca agcatgtgga
    43321 ccttctggtt catgttatcg atgctagcaa tccttaccac gaggagcatg aaaaaacggt
    43381 tctctccatc atgaaagacc tggacatgga agatattcct cacttgacgc tttataataa
    43441 agcggatttg gtggaggatt tcacgcctac ccaaacgcca tataccctca tttctgccaa
    43501 gtctgaggac agtcgtgaaa acttgcaagc attattgcta gataagatta aggaaatttt
    43561 tgaagcattt accctgcgag tgcctttttc aaagtcctac aagattcatg atttagagag
    43621 tgttgcaatt ctggaagaac gtgattatca ggaagacggc gaagtgatta caggctacat
    43681 ttccgagaaa aataaatgga ggttagaaga attttatgac tgatattaaa actttggctc
    43741 taaagtatgg aggttataca agtctggaca aggtctatct ggatcagctt ctagctggta
    43801 aaacagatca ggagcagtta acacttatta cacctccgcc aagtgttgtt aatgcctact
    43861 ttgcagaact ctatcagaaa aagagtcctc aggctgcgac ggattatttt gcagaactca
    43921 gtcaggaact gaatctttac aatactgagc caagtttcac cctagaaaat aagcctttta
    43981 ttcggcttaa tctgtctggt aaatcctttg gtttttgtta tgagagtgac ggccttggtc
    44041 ggattttctt tgaaaatgag gagaaaatct cggatgactt gctctttgaa attgcgcaaa
    44101 ttttccccca tcaattagtc tttgaagagt ctggtaagat ttacatgaag cctgtcgagg
    44161 acgaggaagt tatcagccta gaaagtctca cagctttgac tgatttggaa agattggctg
    44221 atggtcgaaa acgtctcaaa ggctacagcc aagaggattt attgcaagaa gctgctgctt
    44281 tttctggcaa acgctatttc cgatcggaaa accgcacagc catgttatat attgattaat
    44341 tagaaagtat cgaatggata ttcaattttt aggaacgggg gctggtcagc cctctaaagc
    44401 ccgcaacgtt tcaagtctcg ccctgaaact tttggatgag attaacgaag tttggctctt
    44461 tgactgtgga gaaggtacgc aaaatcgcat tctggaaacc acaattcgac cacgtaaggt
    44521 cagcaaaatc tttattaccc atctgcatgg agaccacatt tttggtttgc caggtttcct
    44581 ttctagccgt gcctttcagg ccaatgaaga gcagacagat ttggaaatct acggacctca
    44641 aggaatcaag tcatttgtct taaccagcct tcgtgtgtca ggttctcgtc tgccctaccg
    44701 cattcatttc catgagtttg accaagattc tctgggtaaa attcttgaaa ccgataaatt
    44761 cactgtgtat gcagaggagc tggaccacac tattttctgt gttggctatc gtgtcatgca
    44821 aaaggatcta gaagggacgc tggatgctga aaaactcaag gctgctggtg ttccgttcgg
    44881 cccgcttttt ggtaaaatca aaaacggcca ggatcttgtt ttggaagacg gaactgaaat
    44941 caaggcagca gactatatct cagcgccacg tccaggtaag attatcacta ttttaggaga
    45001 cactcgaaaa acgggtgcca gtgtgcgtct ggctgtcaat gcagatgtcc tagttcatga
    45061 gtccacttat ggcaagggtg atgaaaaaat tgctcgtaac catggtcact caactaatat
    45121 gcaagctgca caagtagcgg tagaagcagg tgccaaacgc ctcctactca accatatcag
    45181 tgcccgtttc ctctcaaaag atattagcaa actcaagaaa gacgctgcca caatttttga
    45241 aaatgtccat gtggtcaaag acttggaaga agtggaaatc tagcagtcac agaaaggata
    45301 agtatgccta ctattctcat taccggagct agcggtggtc tagctcaaga aatggtcaaa
    45361 ctcctgccca atgaccaact catcttgctt ggtagaaata aggaaaaatt agcccaactc
    45421 tacggaaatt attcccatgc agaattgatt gaaattgata ttaccgacga ttcagcccta
    45481 gaagctctgg taactgatct ttatctccgc tatggcaaga ttgatgtctt gattaacaac
    45541 gctggttacg ggatttttga gggatttgac cagattgctg ataaagatat tcaccagatg
    45601 tttgaggtca atacctttgc cctgatgaat ctgtctcgtc accttgcggc tcgtatgaag
    45661 gaaagcagca aagggcatat catcaacatc gtcagcatgg caggtctaat agctactggc
    45721 aagtctagtc tttactcagc gaccaagttt gcggctattg gtttttcaaa tgctctgcga
    45781 ctcgaactta tgccctatgg agtctatgtg acaacagtca atccaggtcc aatccgaaca
    45841 ggatttttcg accaagccga cccagatgga acttatctta aatcggttga ccgcttcctg
    45901 ctagaaccag atgcagtggc taaaaagatt gtcaagatta taggaaaaaa caaacgagaa
    45961 ctcaatctcc cgattttgtt gaacctagct cataagtttt ataccctctt tcctaagcta
    46021 gctgataagt tggcagggga aacttttaat tataagtaaa aagaaccaat gtgcaggttg
    46081 ttgctagcct acatattggt tcttatcatt tctcaactat caaactgaac ttcttctagg
    46141 agatagtcta taaagcgttc gcccatctta gataggctgg ttttctcatg ctggatatag
    46201 accagctcga tcgggtcatc aatatccagt gggatagaaa cgatattgtc tccgtttagg
    46261 ttgctgttca aaatccctgt cgcaatggta taaccatcca aaccaatcaa gagattaaag
    46321 agggtggcac ggtcactgac cacaatggat ttcttgtggt gttcttgaga aagaatctct
    46381 tctgaaaagt agaaggagtt gtgcgtccct tggtcatagc tgaggtaagg gaaattctcc
    46441 aaatcagaca gtttcacctt gtctttcttt gccagagggt tggtcttgct gacaaagata
    46501 tgtggttgcg ctgtgaagag atggtgggct agcaggtgat tgtcatccag catcttggtt
    46561 aaaacatcac ggttgtaact gtttaagaag aggaccccga cctcactgcg gaagttcttg
    46621 acgtcgtcga taatctccca agtccgagtt tcacgaagga agagttcgta tttctccata
    46681 tcgcttttct tgagcaaaga gacaaaggca ttgaccacaa aggcatagtg ttgagacgaa
    46741 acgctaaata gttcgcggtg ggcgacagga tttttatagc gttcctccag aagctgggtc
    46801 tgctcgacaa cctgacgggc ataagagaga aactccatcc catcacgggt caaggtgatt
    46861 cccttgggat tgcggataaa gatctcaatg cccatttcat tttccaaatc tcgcactgca
    46921 ttggagagac ttggctgagt gataaagagt tgcttggctg cctcattcat ggagccagtt
    46981 tcgacgattt tgataatata atgtaattgt tgaattctca tgtttttatt gtaccatact
    47041 tgccttagaa tcgctaaaga agataggaaa ataaggagca ttgtgtgagt ctcttcttga
    47101 aaaaaacaca aaattttgct agaatgagtc ttatgaaaac attctatgat gtgcagcaat
    47161 tcctcaaacg atttggtatt attgtttaca tgggaaaacg cttatatgat attgaactga
    47221 tgaagttgga actatctcgg atttacgatg cagggttgat ggataaatta gactatctag
    47281 aggcagaagc agttcttcgc agagagcaca aggtagaatt ggattatatt gagaaaaatg
    47341 gagaaaagaa ctaatggtta cttggatttt gtgggcactt atactagcaa tgttggcgtg
    47401 gatgggcttt aactatcttc gtattcgccg tgcggctaaa attgtggaca atgaggagtt
    47461 tgaagccttg attcgtacgg gtcaattgat tgatttgcgc gacccagcag aattccacag
    47521 aaaacatatc cttggtgcac gcaatattcc ttcaagtcag ttgaaaacta gtcttgcagc
    47581 ccttcgtaaa gataaacctg tccttctcta cgagaaccaa cgtgcgcaac gagttacaaa
    47641 tgcagctctt tacttgaaaa aacaaggttt ttctgagatt tatatccttt cttatggctt
    47701 ggattcttgg aaagggaaag tgaagactag ctaaccttat ttccaaatta gtcgaatgat
    47761 aggatgaagc tattgtttta tagtataatt aatttgtata aaatttaagg agttcttaga
    47821 acataaaaga aaagaagaaa aatccacttt ttgtgggtat cttgtcgatt attcttggtt
    47881 tgttatttcc aattgtaggc ctgattttgg gtatcatagg attggtcttg gctatttcat
    47941 accaaaaaga gtcataatta gactataaaa tagaaaagat tcttaatatc ttaggaattg
    48001 tgatttctgt agttaactgg attgtagcaa tcgccttaat ttttcggtaa ataaaagaaa
    48061 aaagctttag ttttaaagct ttttttgttt tatcgactca tctctaaata gtttttaatg
    48121 atttgtaact ctttttggtt caaagggcgg tagttacttt ctgctaaatc tgggttcaat
    48181 gtgaagtccc caaattggat tctcttgagg atagtcacct taacaccaac cgagaggaac
    48241 attttcttga tttgatgaaa ttttccttct gaaatggtga tagaggcttg actgatggag
    48301 ggacttgcag atagaatctc tagttttgcg ggtttacaga cagtaccatc taaaaagaca
    48361 attccctttt gaaaggcttg gatatggtca ggtgttagaa gtccattaac ctcaacttga
    48421 taagtcttat cgacatgata ttggggatgg aggagttgga agcccaaggg accgttatcg
    48481 gtcaagagga ggagtcccgt tgtatctcgg tccagtcggc caacggcata gagcttgtca
    48541 gactggatgt cagatggaag caggtccatg acggtcggaa gttccttgtc tttgttggct
    48601 gtaacgacac cagcaggttt atgaagcata agataggtgt gttcatagcc ttgaatgatt
    48661 cgatcctgaa aaaggagttc ttgtagtcct gtatcgatat tttgagctag ggagcgggct
    48721 gggcaaccat cgactagaat tttccctctg aggagtgctt gcttcatggc ctttcggctg
    48781 attttttctt gggctaataa attatctaaa cgcatactgt gcctatctta tcataagtcg
    48841 cttgctttga aaaggcttga cgtgaaaaga aaagcggagt gaaaacattt acacttgctt
    48901 gagttatgtt atttatttga aattatggta taatcgttca gttagaaaat aaattttgaa
    48961 tattatagag gaaatcatga caaaattaag agaagatatc cgtaacattg cgattatcgc
    49021 ccacgttgac cacggtaaaa caaccctggt tgacgaatta ttgaaacaat cagaaacgct
    49081 tgatgcacgt actgaattgg cagagcgtgc tatggactca aacgatatcg aaaaagagcg
    49141 tggaatcacc atccttgcta aaaatactgc cgttgcttac aacggaactc gtatcaacat
    49201 tatggacaca ccaggacacg cggacttcgg tggagaagtt gagcgtatca tgaaaatggt
    49261 tgacggtgtt gtcttggtcg tagatgccta tgaaggaacc atgccacaaa ctcgtttcgt
    49321 attgaaaaaa gccttggaac aagaccttgt cccaatcgtg gttgttaaca aaatcgataa
    49381 gccatcagct cgtccagcag aagtagtgga tgaagtcttg gaacttttca tcgagcttgg
    49441 tgcagatgac gaccagcttg atttcccagt ggtttatgct tcagcgatca acggaacttc
    49501 ttcattgtca gatgatccag ctgaccaaga agcgactatg gcaccaatct ttgacacgat
    49561 tatcgaccat atcccagctc cagtagataa ctcagatgag cctttgcagt tccaagtgtc
    49621 acttttggac tacaatgact tcgttggacg tatcggtatc ggtcgtgtct tccgtggtac
    49681 agttaaggtt ggggaccaag ttaccctttc taaacttgac ggtacaacta aaaacttccg
    49741 tgttacaaaa ctcttcggtt tctttggttt ggaacgtcgt gaaatccaag aagccaaagc
    49801 gggtgacttg attgccgttt caggtatgga agacatcttt gtcggtgaaa ccatcactcc
    49861 gacagatgca gtagaagctc ttccaatcct acacatcgat gagccaactc ttcaaatgac
    49921 tttcttggtc aacaactcac catttgctgg taaagaaggt aaatgggtaa cttctcgtaa
    49981 ggtggaagaa cgcttgcagg cagaattgca aacagacgtt tcccttcgtg ttgacccaac
    50041 tgattcacca gataaatgga ctgtttcagg acgtggagaa ttgcacttgt caatccttat
    50101 cgaaacaatg cgtcgtgagg gctatgaact tcaagtatct cgtccagaag ttatcgtaaa
    50161 agaaatcgac ggtgttaaat gtgaaccatt tgaacgtgta caaatcgaca ctccagaaga
    50221 ataccaaggg tctgttatcc aaagcctttc tgaacgtaaa ggtgaaatgt tggatatgat
    50281 ttcaactggt aatggtcaaa ctcgtttggt cttccttgtt ccagcgcgtg gtttgattgg
    50341 atactcaact gagttcttgt caatgactcg tggttacggt atcatgaacc ataccttcga
    50401 ccaatacttg ccattgattc caggggaaat tggtggacgt caccgtggtg cccttgtttc
    50461 tatcgatgct ggtaaggcta caacttactc aatcatgtct atcgaagaac gtggtacgat
    50521 ctttgtcaac ccaggtactg aggtttatga aggaatgatc atcggtgaaa actctcgtga
    50581 aaatgacttg acagttaaca tcacgaaggc aaaacaaatg accaacgttc gttcagctac
    50641 taaggaccaa acagctgtta tcaagacgcc tcgtatcttg acacttgaag agtctcttga
    50701 gttcttgaac gacgatgagt acatggaagt aacgcctgag tctatccgtt tgcgtaaaca
    50761 aatccttaac aaagcagagc gtgagaaagc taacaagaag aaaaaatcag ctgaataaga
    50821 gctagaaaga gagaaagatg gtctatttag tcctaggaat tttactgctc ctactctatg
    50881 tatttgcgac accagaaagc attaaaggga cagtcaatat cgtcgctatg gtatgtattt
    50941 tagtggcact cttgatttta ttggttctat cttttctgaa aatttttcaa ttaccaacag
    51001 aaatattcct agcaatagcc atgttgctcc tagcttactt tagtgttaga gacattacac
    51061 tcatgccagt caaaaaaagt aaaagaagat aaaaaagaga gttttggctc tcttttttgc
    51121 taggaatttt acaagattgc aatttatttt tgagttatca ccatctcttt tgtttggata
    51181 atatgaatgg gaaaaacttg aataaaattc gttaatggca tctattttat atttggttag
    51241 tactggttgc atattatttt gaaataaaac tactatataa tattattcat agaggtaata
    51301 tagtaaaata tctaaaaaat tcgatttttt ccttgacaac aaataataaa aatgttattc
    51361 ttatcacata agaaagcggt ttcaacaaaa gagagaggag gatttatttt gttgcattgt
    51421 tgctcaaggt gtgatttttt tgaaagaaaa aataaaatga aaacgaaaaa acatagatta
    51481 cttgctctag ctcttatttc aagttttaca ttattgggag ctgcatcagc tgctgtataa
    51541 tatccagatg gaggagtatg gacatatgga gaaggttcag gaggtggtta ggctttttca
    51601 aattactatc atggtaaaaa atatcattat tcatccatcg tgagcagatg ggatggtcat
    51661 tctgataaag gagaagctcc tgctggaaaa acctcctatg catggatttg gactaaatgg
    51721 ggagaacaag tagcatttta ctgtgattat gactaatcgt tcattgattt aaatcgttcc
    51781 ccttgtaaga aattacaggg ggaattttgt ttacaaggag tctaccaagg agtctactat
    51841 gaagaaacta ttcatattat tatcaacttt ttttctcagc ttcttccttg cttggattat
    51901 tgtcttacgt gcgccacaat atttatatgc aagctatgat tccgtttctt tacttcgtgt
    51961 caaaaaagat actcaggaac cgacgcgtga ggtatttgaa caggaattgg agaattttgc
    52021 aaactcagaa cagagtttaa tagctagaag aattgtagag ccgagtaagg atgggacgac
    52081 tcactttact tatgcaactt atggtcaggg aactttacca aaagaattcc aagaagctag
    52141 ccaagaaagt cgtgaacgta gtgatccgct aaatagttat ctccttttgt caggctcctt
    52201 gacgaaagaa aagcttgccg ataaattagg agatttgggt tataaagcaa gtgctgaccg
    52261 aaagataccg ccctattttc ttgcttttcg aatattacta aatcccctta ttttaattag
    52321 tttagcaata tttggcttat ctttctttgc tttagtgatt atcactcgga ttaaggaaat
    52381 gagagcagca ggtataaaac tcttttctgg tcagactctc ttatccatca tggggcattc
    52441 tttatctact gatatcaaat agctccttct atcagccctc ctttccttcc taggtggggg
    52501 agtcgttctt tttagtcaag gtttgtttta tcctatcttg ttagccacct atggttttgg
    52561 gattagtttc tatctgttgt ttttattggc gatttcaatt ttactaatgc ttctttatct
    52621 aatgagtttg agttacaaag cattagttcc cgttattaag gggagattac cccttaaacg
    52681 cctgatgatt ttaaccctat tgtgtcagtt ggttgctgtc tttacagtag gatacgctgt
    52741 taagacaggt ttgacgtctt accaacgatt gaaagaactt gaaatttcaa aacaagcatg
    52801 gcaggataga gcagactatt atcaaatttc ttttggctta ggtgatagag gaaaagatac
    52861 agaaaatcag agcaagtggt atgcctttgc caaggaagca atcgaagagg aacaagctct
    52921 ttatgtaaag gataatctgc tccattttgc caatccacaa ggaaaaaatg aacagggaga
    52981 gacactggat acctatagtc cagatgctaa tacgctctat gttagtccca gttatttgga
    53041 caaggaaaag gtcgtggtag atgctgagac caaacagaag ttagcccatc tccaaaaagg
    53101 tgagtttatc ctcttgctcc cagaacattt gcgctctcga gaagcagaac ttaagaaagt
    53161 ttttgaagaa agattgagtt attatggaaa atctggtgag gaggcaagtg ctcctttgga
    53221 ttatgagatg aaagcgcacg ttagttatct ttcaatggga gaaaagcggt ttgtttataa
    53281 taacggtgag aatcccgtat ctactcagta tttgactgat ccgattttag tggtattcac
    53341 gccgacttct acaggtgata gttttatatc cttatctagt tggtctatca atgctggaaa
    53401 acaactcttt atcaaaggat atgagagtgg gctagaactc ttgaagaaag ctggaattta
    53461 tgagcaagta tcctatctta aagaaggaag aagtgtttat ctaactcgtt ataatgaagt
    53521 tcaaactgaa acagcaactt taatcttagg agctattgtg gggatagcta gttccttgtt
    53581 actattttat tctgtcaatc ttctatattt cgagcaattc cgccgagata tcttgattaa
    53641 acgaatttca ggtttacgat tttttgaaac acatgctcag tatatggtta gccaatttgc
    53701 cagttttgta tttggtgcta gtctctttat tttaagcagt cgagacttgg tgattggctt
    53761 gctcacttta ttggtctttc tagctagtgc agttttgacg ctttaccgtc aagcgcagaa
    53821 agaatctcgt gtttctatga caattatgaa aggaaaatag gatgattgaa ctaaagaata
    53881 tatctaaaaa atttggaagc cgtcagctat tttcagatac gaatcttcat tttgaaggtg
    53941 ggaaaattta tgccttaatc ggtacaagtg gctgtggtaa gacaacactc ttgaatatga
    54001 ttggacgatt ggagccatat gacaaagggc aaatcatcta tgatggcact tctcttaagg
    54061 acatcaagcc ttctgttttc tttagagatt acttaggata cttatttcaa gattttggct
    54121 taattgaaag ccaaaccgtc aaagagaatc tcaatctggg tttagttggt aaaaagttga
    54181 aggaaaaaga gaaaatctct ttgatgaaac aagctctaaa ctgtgtaaac ctctcttatt
    54241 tggatttgaa gcaacctata tttgagttat caggaggaga agcacaacgt gttgcactag
    54301 cgaagataat tttaaaggat ccgcctttga ttcttgcaga tgaaccaacc gcttccttag
    54361 accccaaaaa ttctgaggaa ttactttcca tcctagaatc tttaaaaaat ccgaatcgga
    54421 ccattattat tgcgacccac aatcctctga tttgggagca agtggatcag gtcattcgag
    54481 ttaccgattt atcacataga tgatatggta agattcagtt agaagaaaga gtcacaaaca
    54541 cactttgtgg cttttttatt tccataaaaa tggtaaaata gtaggagtag aaatggagtt
    54601 cgagacatga aagtaataga tcaatttaaa aataagaaag ttcttgtttt aggtttggcc
    54661 aagtctggtg aatctgcggc tcgtttgttg gacaagttag gtgccattgt gacagtaaat
    54721 gatgggaaac cattcgagga caatccagct gcccaatgtt tgctggaaga agggatcaag
    54781 gtcattacag gtggccatcc tttggaactc ttggatgaag agtttgccct catggtaaaa
    54841 aatccaggta tcccctacag caatcccatg attgaaaagg ctttggccaa gggaattcca
    54901 gtcttgactg aggtggaatt ggcttatttg atttcagaag caccgattat tggtatcaca
    54961 ggatcgaacg gtaagacaac cacaacgact atgattgggg aagttttgac tgctgctggg
    55021 caacatggtc ttttatcagg gaatatcggc tatcctgcca gtcaggttgc tcaaatagca
    55081 tcagataagg acacgcttgt tatggaactt tcttctttcc aactcatggg tgttcaagaa
    55141 ttccatccag agattgcggt tattaccaac ctcatgccaa ctcatatcga ttatcatgga
    55201 ttatttgagg aatatgtagc agccaagtgg aatatccaga acaagatgac agcagctgat
    55261 ttccttgtct tgaactttaa tcaagacttg gtaaaagact tggcttccaa gacagaagcc
    55321 actgttgtac cattttcaac acttgaaaag gttgatggag cttatctgga agatggtcaa
    55381 ctctacttcc gtggtgaagt agtcatggca gcgaatgaaa tcggtgttcc aggtagccac
    55441 aatgtggaaa atgcccttgc gactattgct gtagccaagc ttcgtgatgt ggacaatcaa
    55501 accatcaagg aaactctttc agccttcggt ggtgtcaaac accgtctcca gtttgtggat
    55561 gacatcaagg gtgttaaatt ctataacgac agtaaatcaa ctaatatctt ggctactcaa
    55621 aaagccttat caggatttga caacagcaag gtcgtcttga ttgcaggtgg tttggaccgt
    55681 ggcaatgagt ttgacgaatt ggtgccagac attactggac tcaagaagat ggtcatcctg
    55741 ggtcaatctg cagaacgtgt caaacgggca gcagacaagg ctggtgtcgc ttatgtggag
    55801 gcgacagata ttgcagatgc gacccgcaag gcctatgagc ttgcgactca aggagatgtg
    55861 gttcttctta gtcctgccaa tgccagctgg gatatgtatg ctaactttga agtacgtggc
    55921 gacctcttta tcgacacagt agcggagtta aaagaataaa atatgaaaaa aattgtcttt
    55981 acaggtgggg ggacggttgg acacgttacc ctcaatcttt tgttaatgcc caagttcatc
    56041 gaagatggtt gggaagtcca ctatatcggg gacaagcgtg gtatcgaaca ccaagaaatc
    56101 cttaagtcag gtttggatgt cactttccac tccattgcga ctgggaaatt gcgtcgctat
    56161 ttctcttggc aaaatatgct ggacgtcttc aaagttggct ggggaatcgt ccaatcgctc
    56221 tttatcatgt tgcgacttcg tccacagacc cttttttcaa aggggggctt tgtctcagta
    56281 ccgcctgtta tcgcagcgcg tgtgtcagga gtgcctgtct ttattcacga atctgacctg
    56341 tctatgggct tggccaataa aatcgcctat aaatttgcga ctaagatgta ttcaaccttt
    56401 gagcaagctt cgagtttgtc taaggttgag catgtgggag cagtgaccaa ggtttcagat
    56461 caaaaaaatc cagaaccaga tgaattggtg gatattcaaa cccactttaa tcataaattg
    56521 ccgactgtat tgtttgttgg cggttctgca ggtgctcgtg tctttaacca attggtgaca
    56581 gaccataaga aagaactaac agagcgctac aatattatca atctaactgg agattctagc
    56641 ctgaatgagt tgagccaaaa tctttttcgt gttgactatg tgaccgatct ctatcaaccc
    56701 ttgttggaat tggctgatat tgttgtgaca cgaggtggtg ccaatacgat ttttgagctc
    56761 ttggcgatag caaaattgca tgtcattgtg ccgcttggtc gtgaagctag tcgtggtgac
    56821 cagattgaaa atgcagctta ctttgtaaaa aaaggctatg cagaagacct tcaagaaagc
    56881 gatttgacct tggatagttt ggaagagaag ctttctcact tactaagtca caaggaagat
    56941 taccaagcta agatgaaagc ttctaaggaa ttgaaatctc tagcagattt ttatcaattg
    57001 ttgaaaaaag atttatcata aggaaagtaa atgtcaaaag ataagaaaaa tgaggacaaa
    57061 gaaatcctcg aagaattgaa agagttatca gaatggcaga aacgaaacca agaatatcta
    57121 aaaaagaagg ctgaagaaga agcggctcta gctgaggaga aggaaaagga aagacaagct
    57181 cggatgggag aagaatctga gaagtcagag gacaaacagg accaggagag tgaaacagac
    57241 caggaagatt cagaatcagc taaggaagag tctgaagaaa aagtagcatc ctcagaggat
    57301 gacaaagaga aagaagagaa agaagaacca gagtctaaag agaaggagga acaggataaa
    57361 aagcttgcta aaaaggctac aaaggaaaaa ccagccaaag caaagattcc tggtatccat
    57421 atcttgcgag ccttcacgat tttatttcca agtctgcttt tattgattgt ctctgcctac
    57481 ttgctcagtc cttatgcgac catgaaagat attcgtgttg agggaacggt gcaaactaca
    57541 gctgatgata ttcgacaggc ttcaggcatt caggattcgg attatacgat taaccttctg
    57601 ctagacaagg caaaatatga aaagcagatt aagtctaact attgggttga atcagctcaa
    57661 cttgtctatc aatttccaac taagttcact attaaggtca aggaatatga tattgtggcc
    57721 tactatattt ctggtgaaaa tcattatcct attctttcca gtggtcagct tgagactagt
    57781 tctgtgagcc tgaacagttt accagaaact tatttatcag ttctctttaa tgatagtgaa
    57841 caaatcaagg tttttgtctc agaacttgct caaattagcc cagaactcaa ggcggctatc
    57901 caaaaggtgg aattagcccc aagcaaggtg acatccgatt taattcgatt gactatgaat
    57961 gatttggacg aagtcttggt tccgctttct gaaatgagca agaaactgcc ttattatagt
    58021 aagattaagc cacaattgtc agaaccgagt gtgattgaca tggaagctgg aatttacagt
    58081 tacactgtgg cggataaatt aattatggaa gctgaggaaa aagccaaaca agaggctaag
    58141 gaagctgaga aaaaacaaga agaagaacag aaaaaacaag aggaagagag caatcgaaac
    58201 caaacgaccc agcgctcatc gcgtcgctag gtttaccttt tctcttatag ttctttagtg
    58261 accatgtttt tacttgacaa gtgctagtag aaataataga atagtaaaaa acctttaaag
    58321 cagtccagag aggcagctaa ggttagacgg tgaaagggtg gagactaccc atttttcgtg
    58381 gaaccttgct gttggcaggt tccttttttc gtggcttctg ttggccagac tctctcacta
    58441 gtaaaggtaa aaggagaaac ctatgcgaga acatcgtcca atcattgctc ttgattttcc
    58501 tagttttgag gcggtcaagg aatttttagc tcttttccca gcagaagaaa gcctttatct
    58561 caaggtaggg atggagcttt attacgcagc ggggcctgag attgtgtcct acttaaaagg
    58621 tttgggtcat agtgtctttt tggatctcaa acttcatgac attcctaata cagtcaagtc
    58681 agccatgaag atcctatctc agcttggtgt ggatatgacc aatgttcatg cggctggtgg
    58741 tgtagagatg atgaaggctg cgcgtgaagg tcttggaagt caagctaaat tgatcgctgt
    58801 aactcagctc acatcaacgt cagaagctca gatgcaggag tttcaaaata tccaaaccag
    58861 tctgcaagag tctgtgattc actatgccaa gaagacagct gaagctggct tggatggtgt
    58921 tgtttgctcg gctcaggaag tacaagtcat caagcaggct accaatccag attttatctg
    58981 tctgacacca gggattcgtc cagctggtgt tgcagttgga gatcaaaaac gagtcatgac
    59041 acctgctgat gcctatcaaa tcggcagtga ctatatcgta gtgggacgtc ccattaccca
    59101 agctgaggag cctgttgcag cttatcatgc catcaaggat gaatggacac aggactggaa
    59161 ttaaagaact agattagaaa aataaaagga gaataccatg acacttgcta aagatatcgc
    59221 tagccacctc ttgaaaatcc aagccgttta cctcaaacca gaggaaccct tcacttgggc
    59281 atctggtatc aagtcaccga tttacactga taatcgtgtg acactagcct atccagaaac
    59341 tcgtacccta attgaaaatg gttttgtgga agctatcaaa gaagcctttc ctgaagtaga
    59401 agtgattgca ggaactgcaa cagcagggat tccacacgga gccattattg ctgataagat
    59461 ggacttgcct tttgcctaca tccgtagcaa accaaaagac cacggagctg gtaatcaaat
    59521 cgaaggtcgc gtagctcaag gtcaaaaaat ggtagtggtt gaagacctta tttcaacggg
    59581 tggttcagtt cttgaagctg tagcagcagc caagcgagaa ggagcagatg tgcttggagt
    59641 tgtagcgatt ttcagctacc aattgccaaa agcagataag aactttgcag atgctggtgt
    59701 taaacttgtg acgctttcaa actatagcga tcttatccat ctagcccaag aagaaggtta
    59761 catcacgcca gagggccttt atcttctaaa acgctttaaa gaagaccaag aaaattggca
    59821 agaaggttag gtcagtaaga taaagagaga cgaggctacc gagcctcttt taccattttg
    59881 tttaaaatat gacaatattt taaacaaaat aattgacaac gctttctttt gttgatataa
    59941 tgaaaatata atatgtatca aattgatcaa tcaactatct tatttatcac ttcttaggag
    60001 gacgatgtca tgaaaagaag aaaaatatat tccctttttt gcgtagttac aattgcatct
    60061 agtttgtttc tccctacagt tttagctaga cagattggag attatgattt aacgttgcca
    60121 cgttggggtt cggatacaac tagtgagctt gagaaagaaa acgcctctgc tggaattaat
    60181 aataatgaca gtactggtgg cggtaaaaca ttaaatacct ctattcgtag cgcctatagt
    60241 gggtcagata ttactccggt atattcattg gggtctggct ctaggattgt catgtactat
    60301 aatggaggtg gtgacaatta tattggttct ggtactagat tagctatggc gccacaattt
    60361 ggaaatcatg taagaattca tacttcaggt tcttggagtc cagattctta ttaacttact
    60421 tgtcagagta agccttaaag atggttgatt gtgggtgtag catgaaaaaa gaatgctaca
    60481 ccctattttt attataagga ggagtaagga tggaattttt catttgtaat cttgtacgag
    60541 tcgttcaatc acctcgattt tatatgtctt tatttttgac ccttctttgc atgagtttag
    60601 gaaatttcct tgctttcaat ggtatttata aaattgaagg tttatcgatt ttttttgccg
    60661 cttcttctat tcgaggattt tcaccgatta gcctagtagc tgcacttatc tgtacactgc
    60721 cctattctag tcagataata gaggatgctg agagtcattt tctaacagca caattgtgcc
    60781 gaatttctaa aaagaggtat ctagctattg taggtagtac tgcaattatt tcttcttttc
    60841 tagtcttttt tctcccctat ttattattat taggaattaa tcttttagtg actccttatc
    60901 aggaaattta tattggagat tatagtggtg ccttaaaaga attatttgat tccaatcagt
    60961 tgctctatag tcttgtgacg actctctggt atggagtttg gggcgctgtg ttctctattt
    61021 ttggactagc tagtgctttg ctagtgaaga aaaaattagg agctattttc atcccagttg
    61081 cctatatgat ggttggtggt attttatggg ctattttagg gctatcttac ttagaacctg
    61141 tgacaacgct agctttggga tatcagaaag atatcagtct ttccttagtt agtgctcatc
    61201 ttgcttttat tttatttgtt agttgtttgg ttgtttatgg tacatttttt ctacattcag
    61261 aggactatgt ataatgaaac aatttgttca attttataaa aaagatttct tagcagtatt
    61321 ggtttatttt atattactgc tatcctgtgt tttatctagt acagtatatt tattgcgctg
    61381 tcgccaatat tcaatccatc caaatgtatt agaatggatc ttagttttac ttcaagatat
    61441 gacgactgga gtatattgct ttccgttcac atatatattg ttcttttttt atttgatgaa
    61501 taactatttt aataggttgg agtgtcgcat tcgtctgaaa tcaattaagc actttaccag
    61561 ttttagtttc aaattagcag ctcttagtac ggggatttgg acggcgactt tatttttatt
    61621 gatttttcta attgcattta gtaatggttt tagcttctct ttggagataa aggaggttga
    61681 ttttttaaga gaattttatg gtataagtat tgcaaacaat gctagtttct ttataggatt
    61741 ttttttctct tatatagcat actatttctt tttatcctta cttactatta gcagtttttc
    61801 ttggtttaaa aaatcaaaca tgagcttagt atttctgttt acttttttat ttgtagaatc
    61861 cttattctgg atttatcagt tggacaatgg gataattgga ttattgccaa tttttcagta
    61921 tatggtaaat tccaatccgt atgcattgat ttattggctt acattactat ctatcataat
    61981 tccattgact gtattttctg ttcatagaaa ctggaggaga gtgtaaaagt tggaaatggg
    62041 aaagttaagt agtcacatgt ggaggttgaa tcagataatc tataccaagt acttttgggg
    62101 ttatgttctt ttttggatat tgatttgttt aggattatgg tattggttag aaggaaatga
    62161 tagacttgtt atagaaattt taaaagggcc taatctgagt caaaactctt ttttagtctt
    62221 atctatatgg ttgcttcatt ggtttattat tcatacattt tttctagcag ttgtatatcg
    62281 tagaagagca tccgatttct ttatggaagt gattcgattt tcttctatta agctctggat
    62341 taggtatcag atttggacct gttttcttta tggactcatt ttaatcatgg taaaagttct
    62401 agtgattcaa tttatgttac agttaccaaa ctgggatata ggagttttgt ttatagttga
    62461 ttctttgaat gcttgtgtgt tagtcttgtt ttgctttatg ttatacgcac taggagcgaa
    62521 tgtacaaatg aactttgctt gcgttagttt ctttttactc atgattgtgt ttggtggtct
    62581 atttgtagga aatcgaacta attatctatt ttatattttg aatcgaggga atggagatat
    62641 aggacgagat ttatttcttc agttgctatt tttagtcttt ctttttaaaa gcattttcta
    62701 tttcactcgt caaaagagga ggtttataga atgaatgaaa taattacatt aaaaaatatt
    62761 gagttgaaat taaaaaaaac atgtgttttt caaaacctta attttagctg taaacagggg
    62821 gaaattatag gaattactgg tgcgaatggc tcaggtaaaa gtgtattgtt taaattaata
    62881 gctggtttat atagtccgtc ttatggagaa gtgttaatca atggggaaaa tattgttcct
    62941 gagagaaaaa ttccagctaa tttgggagct ttgattgaag aacctggttt tataaattat
    63001 tatagtggct ttaagaattt acaatatttg gcaagcatac gaggagtagt tggtaatcag
    63061 gaaatcaatg atacactgaa aatagttggt ctatatgagc aaaaagacca aaaagttaaa
    63121 acttattcgc taggtatgag gaaaaagcta gggattgctc aagcaattat ggagaatccc
    63181 tctattcttt tactagatga acctatgaat gccttggata aatcaagtgt agaaaatatg
    63241 agaacattgt ttagaaagct ctctagtgaa aaagggacaa caattttgat tgctagtcat
    63301 agtgaagagg atattcgtat cttatgtgat aaagtatatg caatagaaga taaagtatgt
    63361 acactgtgtt cagattgaac atcttagata gactcaggat gttataggta aaatcttaaa
    63421 aagggattgg agcttaaaat ccagttcctt ttaagagtta atgactaatc aacttttcca
    63481 ccttcaacaa ccaggtcatc tgctttagca gtgtcaccgt aggttgggtg aagtgttttt
    63541 tcataggcct tgtggaagaa gttttccttg ctaatttttc aatatcttta ttgatgaagt
    63601 ctagcaattc ttggttgcct ttttgaactg ctgccgcaat ggtatcggga tcaccgaggg
    63661 aagtaattcc tacttcaaat cctttatttt caagcgccca agctagaact tccgtattgt
    63721 cagttgaaaa ggcatctcca cgtccgtcaa gaagagcttg gtaagagtca ctgtattggt
    63781 cgtatttttg gagtttgatt tctggatgat tcttttcaaa ataagtctca gcagtcgttc
    63841 cttttgtgac aattaaggtt ttaccttcaa gttgtttgac gtctgtaatg agaccagtct
    63901 taggtgatac gacacccaga gaaactttca tatatggaag ggcaaaatca acttgtttct
    63961 tacgttcgtc agttactgta aagttagcaa gagtaatatc taccttgttt gaaatcaagt
    64021 attccgcacg gttggcagca tcgactgaaa tgtatttaac cttgacacca aggtctttag
    64081 ctagttggtt ccctagttca atatcgtagc cttggtaaga accatcattg tcaacgtagc
    64141 caaacggttt tttatctcca aacacggcga ttcgcagttc accgcttttt tttgatttca
    64201 tcgatagtgc gagctttggc agttgttttt ccagaggatg aaccagcgtt tccacctgag
    64261 ctacaagcag tgataaagat aagggcaaag gcaagtgcta aaacagttaa gagtggtttg
    64321 aatagtttca taagaatctc ctttatagat atgagccaaa ttggctaaag tcaaagacgt
    64381 ttaaaaattc ctgggctcgt ttggtttgcg gattggtaaa gaaggcttga gctgttcctt
    64441 cttcagcgat tttcccttgg tcgaggaaga taatccggtc agcaatggct tgggcaaact
    64501 gcatttcgtg ggttactaaa atcatggtac ggccttcttg ggccaaatca ttgataagtt
    64561 ccagcacctc acgcaccatt tctggatcca gcgaagcagt cacctcgtca aaaaggatga
    64621 tttctggatg cattaggagg gcacggacaa ttgcaacacg ttgcttctgt ccaccagata
    64681 attgacgggc aaagctatgt tgtttatcca gcaaaccgac acgatctagt aattgcaaag
    64741 cttcttccgt tacttctttc ttgtcccttc cttgagtttt gatagggcct aggatgaggt
    64801 tttgtaagac atccagatgg ggaaagagtt cataactttg aaagaccatg ccaatctttt
    64861 ggcgaactag gtgaaaattt tttttatttt caacgataga ctgaccatcc agaaaaatat
    64921 ctccaccttg aatactttct aagccgttga ggcaacgaag gagggtactt ttcccacaac
    64981 cagatggccc taggatgaca acaacttccc cttttttgat ttctagagaa agtccttgga
    65041 ggatgggatt gtctccgaag gattttttta gttccttgat ttctaagata gtttcagaca
    65101 tttagtttct ccaatgtttt tctaagtgag tggatagttt ggaaataggg tagcaaactg
    65161 cgaaatataa gactagaatg gttccataaa tccaaaatga agcagttggg atggtcaggc
    65221 gattgctatc gatgatttgt tgtccaactt tggtcacttc cacaacccca atcaaaacaa
    65281 ctaatgaagt ggttttaatc atccgagtga caagattgat agcctgcggt agcagtcttc
    65341 ttaagacttg tgggatgatg atgtggtagt aaagttgaac attagtcaag ccgagtgcct
    65401 gtccactttc aaactgatgt ttagggagag aagtgatagc tccacgtacc aagtctccca
    65461 tttcagttgt tccccagagg gtaaaaacga taatagctga agtctcacct gagatattga
    65521 tattaaagtt tcgagctaag ccaaagtaaa cgatgaagag tagcaccagc tggggcatga
    65581 tacggataaa gtccagatac aatcgtgtta aaaatcgtat gattctagaa tgggaggtca
    65641 tgatgattcc catgactgtt ccgaacatca tggataagag gacagacagg atggatatct
    65701 caatcgtaac gcccaatccc tgtaagattc tcaggagatt atttccttga aagagtactt
    65761 ggattcccga atcctgcatg gcggagcctc ctttctatcc agctaaagac cagtgagatg
    65821 ggtagcagca tgatcagata agcaactacc aacatagcta gcgcaatgtc tgtctcatag
    65881 tagagaccaa tcaaatcctt ggcgacatac atgaggtcgg ccaaagccac tgctgagaaa
    65941 acagaggttt ccttgataag gaaaatgaca ttggcactaa aggaggggag tgccaccgct
    66001 gttgcttgcg gaagaaccac atagcgaaag acctgtagag gtgtcagacc aatagcgagg
    66061 ccaatctcct gctgggtttg actgatggct tccagcccac ttcggaaaga ttctgccata
    66121 taggatcctc ctaaaaagac aagcccaagc gttgcacaga cttctgaaga taggacaatc
    66181 ccgattcggg gaagaccgaa gtagagaaag aagagttgaa tcaaaagggg cgtattacgt
    66241 gacaattcaa tgtaggctgt cgctacttgc gccaaaacag ggattcgata atgtcggatg
    66301 atactaacga ttaaaccgag cagaaaggat cccaaaattc cccaaactgc aatatgcaag
    66361 gtcagaaaga atgccttttg atatagtggt agatattgtt caacaatgga ccaatccaaa
    66421 aatagaacct cccatctaga aataatacag ttattgtagc acttaaaatc ttctttggat
    66481 aatatctatt ttttattgcc gttataagga ttttttacca tagacataaa atttctgaaa
    66541 tttccaaaca aaatatttga aaagttttga aaaaaagtta agatattttt gtaatataca
    66601 aagtaaacgc ttacttatta a
//
LOCUS       DAVKHZ010000003        61880 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000003 DAVKHZ010000000
VERSION     DAVKHZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.003"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(4..1170)
                     /locus_tag="VWW99_000126"
     CDS             complement(4..1170)
                     /locus_tag="VWW99_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9538031.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(1178..2272)
                     /locus_tag="VWW99_000127"
     CDS             complement(1178..2272)
                     /locus_tag="VWW99_000127"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9538032.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(2429..2974)
                     /locus_tag="VWW99_000128"
     CDS             complement(2429..2974)
                     /locus_tag="VWW99_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538033.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            complement(3010..3549)
                     /locus_tag="VWW99_000129"
     CDS             complement(3010..3549)
                     /locus_tag="VWW99_000129"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538034.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            complement(3558..4103)
                     /locus_tag="VWW99_000130"
     CDS             complement(3558..4103)
                     /locus_tag="VWW99_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538035.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEI"
     gene            complement(4275..6233)
                     /locus_tag="VWW99_000131"
     CDS             complement(4275..6233)
                     /locus_tag="VWW99_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9538036.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(6436..8058)
                     /locus_tag="VWW99_000132"
     CDS             complement(6436..8058)
                     /locus_tag="VWW99_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9538037.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPFYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            8164..9609
                     /locus_tag="VWW99_000133"
     CDS             8164..9609
                     /locus_tag="VWW99_000133"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9538038.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYIAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISLDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FRFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(9655..9849)
                     /locus_tag="VWW99_000134"
     CDS             complement(9655..9849)
                     /locus_tag="VWW99_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9538039.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVSKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(9950..10558)
                     /locus_tag="VWW99_000135"
                     /pseudo
     CDS             complement(9950..10558)
                     /locus_tag="VWW99_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            complement(10583..11095)
                     /locus_tag="VWW99_000136"
     CDS             complement(10583..11095)
                     /locus_tag="VWW99_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9538040.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(11181..12116)
                     /locus_tag="VWW99_000137"
     CDS             complement(11181..12116)
                     /locus_tag="VWW99_000137"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9538041.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(12187..12486)
                     /locus_tag="VWW99_000138"
     CDS             complement(12187..12486)
                     /locus_tag="VWW99_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9538042.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(12476..13225)
                     /locus_tag="VWW99_000139"
     CDS             complement(12476..13225)
                     /locus_tag="VWW99_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9538043.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(13279..13638)
                     /locus_tag="VWW99_000140"
     CDS             complement(13279..13638)
                     /locus_tag="VWW99_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9538044.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(13640..15040)
                     /locus_tag="VWW99_000141"
     CDS             complement(13640..15040)
                     /locus_tag="VWW99_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9538045.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(15335..15574)
                     /locus_tag="VWW99_000142"
     CDS             complement(15335..15574)
                     /locus_tag="VWW99_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9538046.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(15606..16076)
                     /gene="ssbA"
                     /locus_tag="VWW99_000143"
     CDS             complement(15606..16076)
                     /gene="ssbA"
                     /locus_tag="VWW99_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9538047.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(16088..16378)
                     /locus_tag="VWW99_000144"
     CDS             complement(16088..16378)
                     /locus_tag="VWW99_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9538048.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(16531..17877)
                     /gene="asnS"
                     /locus_tag="VWW99_000145"
     CDS             complement(16531..17877)
                     /gene="asnS"
                     /locus_tag="VWW99_000145"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9538049.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIICATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(18034..18258)
                     /locus_tag="VWW99_000146"
     CDS             complement(18034..18258)
                     /locus_tag="VWW99_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9538050.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            complement(18251..19438)
                     /locus_tag="VWW99_000147"
     CDS             complement(18251..19438)
                     /locus_tag="VWW99_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9538051.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEKRLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(19435..19923)
                     /locus_tag="VWW99_000148"
     CDS             complement(19435..19923)
                     /locus_tag="VWW99_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9538052.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            20244..20792
                     /locus_tag="VWW99_000149"
     CDS             20244..20792
                     /locus_tag="VWW99_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9538053.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(21029..27886)
                     /locus_tag="VWW99_000150"
     CDS             complement(21029..27886)
                     /locus_tag="VWW99_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9538054.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKEDVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPRVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAQGAKGEKGAQ
                     GERGLTGAQGAKGEKGDQGERGLTGAQGQAGRDGVTPTVTVKDNKNDGTHTITINDGR
                     GNVTSTVVRDGFDGASPLVATQRNDADKTTTVIFYYDKNGNNELDASDKKLKEVVIAD
                     GAKGEKGDKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITINDGKGGITTTV
                     VRDGFDGASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKG
                     DKGDNGKDGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAERDD
                     AKKQTTLTFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPT
                     TEGKDGDTFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQGERGLTGA
                     QGVKGEKGDQGERGLTGSKGEKGDQGERGLTGAQGAKGAKGEQGLQGRDGAQGPKGAD
                     GQRGPAGPQGPKGEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLA
                     TITDGRNGTNGQSPTITATRSVQNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPT
                     VTITEGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGD
                     KYINTETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGAD
                     GAVGRDGRDGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKG
                     DKGERGEDGKTPEVTVTPGKDDHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLL
                     RVQGKNGNPSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVK
                     DGKETKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDN
                     HDGSQPTTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTPGKDPVTFTV
                     KDGKNGKDGRAPKIKVEDITSPSRIRRATDAAATPTRNGIRVTVYDDVNDNGVYDEGV
                     DKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTA
                     NITTTENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDG
                     NVTNAIIKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTA
                     SVRDNGDGSHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTET
                     IITDGKSPVATVRDNQDGTYTIRVENGNGTVSETTVRDGKSPTAKVVDNGDGTHTITV
                     VNSDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVD
                     NGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKT
                     EMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTVTKTVIKDGKDGRDGRDGRDGKD
                     GKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVKPVPAQPTPNVPTPEVPVKPVPAQ
                     PTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVAPTTGK
                     ENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKED"
     gene            28102..29586
                     /locus_tag="VWW99_000151"
     CDS             28102..29586
                     /locus_tag="VWW99_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9538055.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILEGLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            complement(29637..30365)
                     /gene="yaaA"
                     /locus_tag="VWW99_000152"
     CDS             complement(29637..30365)
                     /gene="yaaA"
                     /locus_tag="VWW99_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9538056.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(30444..30608)
                     /locus_tag="VWW99_000153"
     CDS             complement(30444..30608)
                     /locus_tag="VWW99_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9538057.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            complement(30787..32382)
                     /locus_tag="VWW99_000154"
     CDS             complement(30787..32382)
                     /locus_tag="VWW99_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538058.1"
                     /translation="MKRIVCELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            complement(32394..32804)
                     /locus_tag="VWW99_000155"
     CDS             complement(32394..32804)
                     /locus_tag="VWW99_000155"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9538059.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(32920..33711)
                     /gene="yghU"
                     /locus_tag="VWW99_000156"
     CDS             complement(32920..33711)
                     /gene="yghU"
                     /locus_tag="VWW99_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9538060.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(33724..36420)
                     /locus_tag="VWW99_000157"
     CDS             complement(33724..36420)
                     /locus_tag="VWW99_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9538061.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            36724..37908
                     /gene="mntE"
                     /locus_tag="VWW99_000158"
     CDS             36724..37908
                     /gene="mntE"
                     /locus_tag="VWW99_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9538062.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(38051..39922)
                     /locus_tag="VWW99_000159"
     CDS             complement(38051..39922)
                     /locus_tag="VWW99_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9538063.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMVNQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(39919..41103)
                     /locus_tag="VWW99_000160"
     CDS             complement(39919..41103)
                     /locus_tag="VWW99_000160"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9538064.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(41115..41882)
                     /locus_tag="VWW99_000161"
     CDS             complement(41115..41882)
                     /locus_tag="VWW99_000161"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9538065.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(42148..42996)
                     /locus_tag="VWW99_000162"
     CDS             complement(42148..42996)
                     /locus_tag="VWW99_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9538066.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(42998..43372)
                     /locus_tag="VWW99_000163"
     CDS             complement(42998..43372)
                     /locus_tag="VWW99_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9538067.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(43446..44798)
                     /locus_tag="VWW99_000164"
     CDS             complement(43446..44798)
                     /locus_tag="VWW99_000164"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9538068.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            complement(44822..45601)
                     /locus_tag="VWW99_000165"
     CDS             complement(44822..45601)
                     /locus_tag="VWW99_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538069.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEASITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(45588..46445)
                     /locus_tag="VWW99_000166"
     CDS             complement(45588..46445)
                     /locus_tag="VWW99_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9538070.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            46581..47549
                     /locus_tag="VWW99_000167"
     CDS             46581..47549
                     /locus_tag="VWW99_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9538071.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNEKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(47583..48494)
                     /gene="whiA"
                     /locus_tag="VWW99_000168"
     CDS             complement(47583..48494)
                     /gene="whiA"
                     /locus_tag="VWW99_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9538072.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(48491..49468)
                     /locus_tag="VWW99_000169"
     CDS             complement(48491..49468)
                     /locus_tag="VWW99_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9538073.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(49465..50355)
                     /gene="rapZ"
                     /locus_tag="VWW99_000170"
     CDS             complement(49465..50355)
                     /gene="rapZ"
                     /locus_tag="VWW99_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9538074.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(50407..50787)
                     /locus_tag="VWW99_000171"
     CDS             complement(50407..50787)
                     /locus_tag="VWW99_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9538075.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(50798..51385)
                     /locus_tag="VWW99_000172"
     CDS             complement(50798..51385)
                     /locus_tag="VWW99_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9538076.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(51394..52626)
                     /gene="clpX"
                     /locus_tag="VWW99_000173"
     CDS             complement(51394..52626)
                     /gene="clpX"
                     /locus_tag="VWW99_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9538077.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(52658..52828)
                     /locus_tag="VWW99_000174"
     CDS             complement(52658..52828)
                     /locus_tag="VWW99_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538078.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(52828..53334)
                     /locus_tag="VWW99_000175"
     CDS             complement(52828..53334)
                     /locus_tag="VWW99_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9538079.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFGPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(53464..53982)
                     /locus_tag="VWW99_000176"
     CDS             complement(53464..53982)
                     /locus_tag="VWW99_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9538080.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(54479..55984)
                     /gene="lytC"
                     /locus_tag="VWW99_000177"
     CDS             complement(54479..55984)
                     /gene="lytC"
                     /locus_tag="VWW99_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9538081.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGTRYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(56022..56780)
                     /locus_tag="VWW99_000178"
     CDS             complement(56022..56780)
                     /locus_tag="VWW99_000178"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9538082.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(56878..57555)
                     /locus_tag="VWW99_000179"
     CDS             complement(56878..57555)
                     /locus_tag="VWW99_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9538083.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELFFDASLALECL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(57564..58508)
                     /locus_tag="VWW99_000180"
     CDS             complement(57564..58508)
                     /locus_tag="VWW99_000180"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9538084.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(58690..59202)
                     /locus_tag="VWW99_000181"
     CDS             complement(58690..59202)
                     /locus_tag="VWW99_000181"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9538085.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(59289..60047)
                     /locus_tag="VWW99_000182"
     CDS             complement(59289..60047)
                     /locus_tag="VWW99_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9538086.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKFAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            60255..60401
                     /locus_tag="VWW99_000183"
     CDS             60255..60401
                     /locus_tag="VWW99_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538087.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(60548..61678)
                     /gene="ugpC"
                     /locus_tag="VWW99_000184"
     CDS             complement(60548..61678)
                     /gene="ugpC"
                     /locus_tag="VWW99_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9538088.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
BASE COUNT        17567 a        14023 c        11392 g        18898 t
ORIGIN      
        1 tttttaacgt ttggcaaaag tagccacaat ccgctgacag acttcttgca acagagattt
       61 gggcatagct atattgatgc gggcatggag acttccttcc tctccaaaat ccaaaccacg
      121 gttgaggata accttggctt catttctcaa caactcttgc aatgtttcat cagtcaggtc
      181 ataagctgaa aagtcaagcc aaatcaagta ggtaccttgc ggtttcatga ccttgatttt
      241 agtctctttt ccaaatagat ccaccacata attgatgtgg tcttcaaaga cttgcttgag
      301 ttcctctagc caatctttac cgtatcgata ggcagcttct gtcgccaaat aacccaagcc
      361 tgaaatttca tgctgattat tggccaacag gcgtttctgg aaagctagtc tcaacttagg
      421 attttcaatg actgcatagg aattttttgt tccagcaata ttaaatgttt tagtggcact
      481 gctcaagacg atagcaaaat ttttgaaggc aggattgatg gtattgaaag actggtgttt
      541 gtgaccaaag agggtcaaat cttggtgaat ctcatccgaa actaacaaaa caccgtgttt
      601 ttggcagagt tggccaatct tctccaacac ttctttttcc caaacacgtc caccaggatt
      661 gtgagggttg caaagaatat agagtttaac ctcctcttcc accaaatcct tttcaagttg
      721 gtcaaagtca atctcaaaca gaccatcctt ttccactaag gaattagtaa tcaatctacg
      781 attattcaac ttgacactgc gagcaaaggg tgggtagaca ggcgtgttaa ttaaaaccgc
      841 ctcgccttct tttgtaaagg tttgaatagc tgttgagatg gctggtacca caccctcgat
      901 aaagacaaga gcctctttgt caaagttgta accgtattgt gtagcttccc acttttgaac
      961 ttccttaatt aagtcttcac tggcataggt ataaccataa accagttggt ctgcgtaagt
     1021 ttgcacggct tggcggattt caggcaagac cacaaagtcc atatccgcta tccaagctgg
     1081 tagaacttca ctatccgttt ctgtttcttt ccatttatag gtatggtgcc ctaaacggtt
     1141 gggcaggctt gtaaaatcat attttcccat ctttgtctta tccttctatg gcttggcgca
     1201 aatctgcaat caaatctcta gcatcctcaa tcccaataga caaacgcaag aggtcatctg
     1261 tcaaaccata agaatggcgt acctctgctg gaatatcagc atgagtttga gtcgttggat
     1321 aagtaataag actttccact ccacccaaac tttccgcaaa agagaagacc ttgagactgt
     1381 tcaaaatatg aggaatgcgt gtttcatcgg ctactttaaa ggaaatcatg cctccacgac
     1441 cagtgtagag aacttcctta actgctggag aatccttcaa aaaggcaacc acttcttggg
     1501 cgttagctgt tgagcgctcc atacgaagag acaaggtctt gagaccacga agcaactggt
     1561 agctgtcaaa tggagacaag actgcccctg ttgtattaag attgtaaaaa agcttctcgt
     1621 atagttctaa actattggtc acaaccactc cagccaagac atcattgtgg cctgctagat
     1681 acttggttgc tgaatggaga acgatatctg ctccatcttc aatcggacgt tggtagatag
     1741 ggctatagaa ggtattgtcc accaccactt tggcaccctt agcatgagcc aattttgcta
     1801 gtttttcgat atcaaattcc aacatcaagg gattggttgg ggtttcgata tagagaacat
     1861 ccacatcctt ttctaactcg gcaatcaact cttcttctgt attggcatag gtaaaatgga
     1921 aacgaccttc ctgctccact tggttaaacc agcgaaaaga accgccgtaa agatcacgca
     1981 ctgccaagac cttacttcct actggaaagc cgctaaaggc cagtacaata gctgacatcc
     2041 ctgagctagt cgctaaggca tagtctgctg actcaatagt cgccaagact tcctcagcct
     2101 tactacgagt tggattttta gtgcgcgtat agtcaaaccc agtagatcga ccaaactctg
     2161 gatgctgata ggtcgttgaa aaatgaagcg gtgtcaccaa tgcacctgtc gcttcatctg
     2221 acttaatacc cgcctgggcc aaaattgtgt taatgtgtaa ttccttgctc atacaatacc
     2281 tccaaatcta tagtaactat tgtaccactt attttcatca actcattttc ttcttttcaa
     2341 gagctagtta tagtttcaaa ctatataaaa agggagtttt tcctgctccc tttaatagac
     2401 tataaaatgg tgaatctcaa aagacacttc actctatcat ttgctcctgc acaaaacgag
     2461 cataacgctc atgattttcc agtagttcct tatgagttcc tgagccagtg attttcccct
     2521 cctctaagaa gaaaatacaa tccacatctt ttaccgttga caaactatgc gctataatca
     2581 caaccgtctt ctcctttaat acagaataga ggctactgat aatcgcatac tcagaatccg
     2641 catcaagatt agcagtagct tcatcaaata taagaatttc agcatctttt aagtaggttc
     2701 tagctatttg aagtctttgg ctttgccccc ctgacaagaa tcgtccgcgt tcaccaactt
     2761 cagtatctag tccctctttc atggagcgaa tctcatcacc tagtgatact aagtctagca
     2821 ctttcatcaa ttcatcatca gttactaagc gattcaaacc gagacaaaga ttgtcacgaa
     2881 tactgccaga taagactgca ttattttgtg aaacccaagc gattttactt ctccattctt
     2941 ttaagttaaa atcatatata cttgattgct ccattagaat atctcctgaa agcggtttat
     3001 aaaaccgctc taacaaacgc acaatcgttg attttcctga tccagatggt ccaacaaaag
     3061 caatttttgc cccttgaaaa ttgaacaagt aatatccttt aagacaggtc gattttcatc
     3121 ataaccaaaa tagacatggt taaaattcaa cccttgtcct gataccgatt ttcctccctc
     3181 aaatttttct ttaggaactg caagcaagtt atccagtgca actgaagatc ccttgctcct
     3241 agaataaaca gttacaaaat tagctatatt actaatagga ttaagtaatt gaaagaggta
     3301 aatcaaaaac gaaaccaagg ttcccacaga tatatatcct gcgctgaccc gataaccccc
     3361 ataggttagc atcacagcta tagtcgcaaa gataaataag agagcaaacg gggtctcaaa
     3421 agaagtaacc ctatctgatt tcagtgaatt gttttgtacc ctttcaatac aattatccaa
     3481 aacatcctgt acacttttct ctgcttggtt agtcttaatt aattcatgtt cttgaatctt
     3541 ttcagtcaat tgccctgtta aatttcctcc tgtaaacgac gactatactt ttcactgata
     3601 ttggaaaggg gcaagataat aaacatcata caaggaagag tgatgaataa aagtagagaa
     3661 agattccaat caagactaaa taagactaca atggaaccaa gtaccataac taaactcaga
     3721 ataatatttg ggaaagtcgt aattaaaaac tcacgaatga cactcatgtc attgacaatg
     3781 gcagaagtca actccccact ttggctctta tcaaagaagg atttctctgc ataaatcaac
     3841 ccctctatca cttttttcct gatttttgct atcttttttt cacccgattg actaaacaga
     3901 tagtaaccaa tagaagaaaa caaggcttga ccaataaaaa tcaaaaacga ttgaaatact
     3961 ttggaaccta tattttcaat agaactccca tctattaaac cctttaagat aaggggaagc
     4021 aacaaagcaa gtagactaga cagaacaagt aagaaactcc ccataatcac cttagtatct
     4081 actcttaata attttaattt cataaatact ccttataata tttcaacgga taaagtcggg
     4141 aataactcaa tttgaggata aaatctaata aatcttccta taacaaaacg cataacatct
     4201 aggattttat atacctgata ttatgcgttt ttaagcacaa agacttctta cacaaactta
     4261 tctacaatta gattttattt gacatgtttt gccaattctt cttgggcttt tttattggat
     4321 tcttcttttt ctttcaacca tttttctctg gcttttgcat attcgtctgt tgtgacaatc
     4381 ttatcttgta ctttgaggta tttatatgat tcaacccctt ttgtaccggt taaaccatag
     4441 gcagcagcaa atggtacggt tcttctcaat gatggtgttc ccccacgcga aacacttgga
     4501 agaactaaag aactatcaat caaccaagct tgaatatcag catatttctc ataacgtttg
     4561 gctggatctt gctctttatt agcttcttcc aacatttgag tatagacatc cagtccaact
     4621 gccttagcct tgtcattggc ttctccaggc tctagtccaa gattttgtag aactcctcca
     4681 ctattagtat taaaaatatc gagataggtt gacgggtctt gataatcagg tccccaaccg
     4741 ccatgatata aatcatagtc tttctgagca gctgtttgag caaagtagcc tgaactgtca
     4801 aactcatctg atgttaattg ctgaatgtca atcactacat tatcagaacc taaaacagat
     4861 tcaattgatt gtttgataga actaactcct tgtatgccta ctttatctgt tacttccaca
     4921 gtcttatcca agtggattgg gaattgaaca ccctttgctt cgagttcttt cttagcttcc
     4981 gcaaacttag ccttggcttt ctcaggattg taataagggt cttgaccatc cgcaaagttg
     5041 ataccttgcc attccttacc atagttgacc atcttagagg ctacaacttc accaaagtct
     5101 tttcccttga tactgacaaa gtttggagga accactaggt tacgcaaaat ctttgttgca
     5161 ccttctttcc cttcagactg agccccataa gatgttctgt caaaagcaaa attgatagcc
     5221 tgacggaagt ttttattgag aactgcttcc tgggtcgatt tcttttcaat gtcacttgtt
     5281 ttagaagtat aattgtaaga cttcctatct aggttaaaat taaagaaata tgaagttgaa
     5341 ttttgcatac tatagatgat attgtttttg tatttttctt taatcccttc atagctggag
     5401 ctgttaggaa aaagacgagc cgtagtataa gcaccagctg taaaattacg ttccagtgat
     5461 tcttggtcgc taccatcata gtaggtcaat ttcacatcgt ctacaaagac attcttagca
     5521 tcccagtaat tagggttttt cttatattca atagcagatt ttgagacaag tgctttcatc
     5581 aagaaaggtc cattgtacaa aatactagat ggatccgcct tcccaaaatc atcccctttt
     5641 gatttcagga aatctgcatt aacaggaaaa agtatcgttg caagtgtttt tgaattccag
     5701 taaagttctg gtttaaccaa agtatattga accgtttggt catcaagtgc cttgacaccg
     5761 acagttgaaa agtcgcttgt tttaccagtg atatagtcat ccaaaccagc aacagagtcc
     5821 tgcactagat acaaagcttc tgatttttta tcagctgcat attgcaaacc tgtcacaaaa
     5881 tcctgggcag ttacaggcgc atattcttct ccctcagaag taaaccactt ggcatcctta
     5941 cgaagtttgt aggtataggt caaaccgtcc tgagaaacag tccaatcctc tgctaatgat
     6001 ggaataatat tcccatattg gtcattttct aataacccgt ctaccaaatt tgcaacaata
     6061 tcggatgttg ctgcgcggtt ttctgctaga tagttcaaac tagatggatc acttgaataa
     6121 acatagttat aggtttttga tgcagtgcta gaatttccac acgcgctcaa taaaactcct
     6181 gtacccagga caagacctgc caaggttaga tatttgctct tagacttttt catttccaga
     6241 acctcctgct ttaaatattt gttttattat acaactcttg gtttgtttag tcaattgttt
     6301 tttccaaaaa atcaaaaaaa cctcattttc tatttttgaa agataatttt agctattttg
     6361 acgctaaaaa aggaataagt ttcaagttct cctaaaagat tgttcaactt attcctttta
     6421 tttataaatg ctaccttacg aaagcttaaa ttttgctcgc aggcgatctg cttgggcttt
     6481 tcctattacc ttatctaata aataggtacg cagactcata accatataaa gtccaccccc
     6541 catggcaccg acaagagcta cataaaagaa gctccacaaa cgtccacttg gttggaagaa
     6601 aaatcctaac agccactgga tggttcctat taacagaaac atgactaggg tcagcaaact
     6661 gattaaaatg gttcgcttca aaatcacctt gcgcttgaca ccagttactt tacaaatatc
     6721 ccgatacatc aagacgttag gaatgatgag agcaatggtt gttgaaatca aaggaccata
     6781 actgtggaag agggcgatgg taggtagttg caagactagc ttggcaatag aaccatagat
     6841 aaaatagaga acggccttgc ggttgcggaa catggcctga agcattggag acaagaccat
     6901 gtacaagcct aaaataatag actgcaaaac tgcaaagaca aataagccca gagccaaact
     6961 atctggctta ccatagaaga ccgtataaaa aggttctcct accataacca ctccaaccgt
     7021 tgctggtagc aagaacataa agagtagggt gagactgtcc tgaacgagac gagaagctgc
     7081 tttcaagtcc cccttgacat agttttccgt caaaagtggc aaaccaacac tcccaatcga
     7141 aacccctaca gaaatcaaaa tcatcgtgat tttattagga ttggctgaga aataagaaaa
     7201 catgacaacc aagtcctcat tgctgtagtt ggtaaaccag ctcatactat tgataaaggt
     7261 cagctgatcc aaaatctgga agagctggat ggcagaccct gtcaggataa aaggaatggc
     7321 ttccttaatg gtatcaacca agagacgctt actgttaatc ttatctcctg tttcaaagac
     7381 tcttttgagt gaaccttctt gggcaaggaa ataaatcaag actgcaaaac tggctaccat
     7441 accgacaaag gcagcaaagg ttgattgggt aacggctgct agataatctc ctgaaccgag
     7501 cttcataatg ataaaggttg ctaggagcat ccagataaca cgaatgacct gctcagcaat
     7561 ttggctcatg gcatagggtt tgaggttatt catcccttgg aaaaatcctc ggataacact
     7621 catagacggg aaaatcaaga ctccccaagc caagctttgc atgattggga tcaagtcttt
     7681 gcccacgcca gacaagtctg ctagccaagg agcaaagaca tacaagacta aagcaaaaac
     7741 caggcctagt cctgtcataa agcctaagaa gctccgaatc agggcaaagc tatgctcttc
     7801 ttctcgcatg gtattatact tggcaacttg cttggccacc gcaactggaa tccccgctgt
     7861 tgaaaccaac aagaaccaag catagatatt gtaacccatg gtaaagagac catttgcctt
     7921 agctgcataa gcccccatcc agatgtacca agggataatg taaacagccc cgagtaggcg
     7981 actgataaag ttactagccg ttagccaagc agtcccccgt aacatctggg cctgctggtg
     8041 attgttttcg tgcgacatag attcctcatt caattttgat aactaggaaa aactccttat
     8101 cttccattat aaacttttcc ttgttacttg taaatttgcg actttcggtt tacaatagaa
     8161 agtatgataa aaattgaaac tgtattagat attttaaaga aagatggcct ttttcgcgaa
     8221 attattgacc aaggtcatta ccactacaac tacagcaaag ttgtttttga tagcatcagc
     8281 tacgacagcc gaaaagtaac agaagacact cttttttttg caaaaggcgc tgcctttaaa
     8341 aaagaatacc ttctttctgc tataacacaa ggcttagctt ggtatatagc tgaaaaggac
     8401 tacgaagtcg gtatccctgt catcattgtg aacgatataa agaaagccat gagtttgatt
     8461 gccatggagt tctatggtaa tccacaggaa aaactcaaac tccttgcctt tactggtact
     8521 aagggtaaga caacagcagc ctatttcgcc tataacatct tatctcaagg gcatcgacct
     8581 gctatgttgt cgaccatgaa cacaactctt gatggagaga ctttctttaa gtcagcgttg
     8641 acaacccctg agagtattga cctctttgac atgatgaatc aggctgtgca aaatgaccgt
     8701 acccacctca tcatggaagt ctccagtcaa gcctatctgg tcaaacgtgt ctatggtcta
     8761 acctttgatg tgggagtttt cctaaatatc agcctagacc atatcggccc gattgaacac
     8821 cctagctttg aagactattt ctaccacaag cgtctcttga tggaaaatag ccgagcagtc
     8881 atcattaaca gtgacatgga ccacttctca gtcttgaaag aacaggttga agatcaagac
     8941 catgatttct atggtagcca atttgataac caaatcgaga attccaaagc ctttagattt
     9001 tcagctacgg gtaaactcgc tggagattat gatatccaac tcattggcaa cttcaaccaa
     9061 gaaaatgcag ttgctgctgg acttgcttgt ctccgtctcg gagcaagtct tgaggacatc
     9121 aaaaaaggca tcgctgcaac ccgcgttcct ggtcgtatgg aagtcctcac tcagaaaaat
     9181 ggagccaagg tattcatcga ctatgcccac aatggggata gtctgaaaaa actcatcaat
     9241 gtggttgaaa ctcatcaaac cggaaagatt gctctggttc tgggatcaac aggaaacaag
     9301 ggagaaagtc gtcgtaagga ctttggcctc ctcctcaatc aacaccctga gattcaagtc
     9361 tttctgactg ctgatgaccc taactatgaa gacccaatgg ccattgcaga tgaaattagt
     9421 agctacatca atcatcctgt tgagaagatt gcggatcgcc aagaagccat caaggcggca
     9481 atggctatca caaatcacga attagatgca gttattattg cgggtaaggg agccgattgt
     9541 taccaaatca tccagggcaa gaaagaatcc tacccaggag atacagccgt cgcagaaaat
     9601 tatttataag aatagaaaaa atcaagggaa aatgaaaccc ttgatttttt cctattattt
     9661 agtaaaaacg tttttcaaac cttcaacggc accttctaca gcaccttttg cgtcttctac
     9721 aacgtctttt gccttagaaa ctgttttttc aacagctcct tcaagttctg tcttgctgtc
     9781 tccagtaact ttaccaaaac cttctttgac agcgcctgtt gcttgttcca atttgttttc
     9841 aagtgacata tgattgtctc ctttagttta ttccgatatg tgttaccggt tacatatagt
     9901 ttataccgaa tactaaggaa agtcaaacaa tgtactcaga aacaagaaat cacgctaagt
     9961 agaaagttaa cctctcctta tcaaaatcga tagccaactc atactttcca tcttcatttt
    10021 gcacaatata gcctaagaca acctaactgt ccacaaagat atcacggcat ttctgcataa
    10081 gctggtcttt tttgagaaat ttcaacaaaa aagccgtcat atacttgagg gcatactcag
    10141 gattaacatc tcctaaaatg tcatagagct cctgctgttt ttctgtcaaa ggatactgct
    10201 gtttgacctt gtaaaaataa ttggacaggg tcattttaat gcgcgcaaag tccgtctttt
    10261 caactaaaat agctgcattg atttgattgc gcaattccgt ctcaaaactc tgctccaaca
    10321 aggcttgata gaccggacta gcttctctga caaaaatctc ttgatccagt tcaagactat
    10381 caagtgattc aagcgtagga aaattgaggt aatagcgctt attttcacgt agaatcaagc
    10441 ctgcctttat atactcttcc atgagtttat caactgcaac atctggaaat tgagccttaa
    10501 tttcccgtag aatcacatcc tcatactgat ccagatagcg gatcaattct ccaaaaatgg
    10561 ctgtctcgtc aaacgagatg gattaaaaat ctgaatcatg aagattcctt tctttacatt
    10621 gtaacagagg cgatgctgcc aactgactag gattggtacc agcttggttc agaggaacag
    10681 gcagacctag ttctatgtaa tactccctcc aaaaatcact aatctcctgc tccccatctc
    10741 caaatttcat aggaatagtt tcaaaaatag gtaggatttc tgctatatac tgggagcagt
    10801 aaaaaccagc tgcatctgga tagaaagaag cattgtaggg tgctccaaga tgtttgcaag
    10861 ctctttcctt caccgactgg atatccattt ctgggtaaac atagaggtcg tataaatgat
    10921 tggactcaaa gaagtctgcc ggttcttgac agacaacacc agcctgtcca ctagcatgat
    10981 aaatcatccc atccaaataa atggcaacat ggctatagtt acctgtggaa gtctggatgg
    11041 cctgtcccat gtctgaccca tctctcacaa aaatcaaatc gccattttct aacatacttc
    11101 actcctagaa aaaaggagcc gaaactcctt tcgatataag gcaaactaga ctttccgatt
    11161 ttgagctgac acccaaaatc ttacgcatta aagctttcag tcaattgagg taccacttgt
    11221 ttcttacgtg aaacggcacc agcaaggaag gcatgattgt tttcaagttt gaagttgaaa
    11281 gccgcttcga ccttgtccat attggcacca agagccaaga tttctgagtt tgagttgacg
    11341 atatctgtaa tcatcaagac aaagtcagag tagccgtttg attcgttggc agcttgcatt
    11401 gcagcttcaa tttctgcttg gcgttccaaa acttcagcga tgtcaactgt gttcacttgg
    11461 gcaacacgga cattatttcc gttgagttca aaagtcttag catcgatatc aatcaattct
    11521 tcagcagatt tgctagccaa gttggtacca gctttcaaca ttgccaaacc atattcttcc
    11581 aagttcacac cagccaattc agccaattca ggagcaatga ttttatctgt tgggtgtgtt
    11641 gttggtgatt tcaaaagaag ggtatctgaa atcaaacctg aaagcatcaa acctgcaatc
    11701 tctttaggca cagctacacc atgttctttg aacatacggt aaacgattga agacgctgat
    11761 ccaactggct ccaaacgcat gtaaagtggg cttgcagttt caaagttagc cacacggtgg
    11821 tggtctacaa caccgtaaac ttctacttca gcgatatctg atacagattg ttggaattca
    11881 ttgtggtcag tcaagataac ttgctctgcc ccctctgctt tggcagaagt gataacacgt
    11941 ggtgcttcca caccaaaata gttcaagaca aaggctgttt cttcatttgg agttccaagg
    12001 gcaacagctt ccgtatccaa accgtaagct tcttttgcaa ggtaggcaaa agctacagat
    12061 gatccgatgg catctgagtc tggattttgg tgaccaaata ctagaatctt ggacatgata
    12121 atacctcatt ttgtttattt tctttattgt agcatttttt tcgctttttg acaaaggtaa
    12181 gaggagtcaa aggactcccc acgattcttc aaaataactg agtaaatttc tatcttgatt
    12241 ttcagataaa aattcttcct tctgtggcct cttcttacgc ttgagaagag cttcagccga
    12301 catggcttct tccttactgg caaaaccttg agcatagata agtttgactg gcaagcgtgc
    12361 tcgtgtatat ttggctccct tcccactatt gtggatagcg aggcgtcttc tcatatcagt
    12421 cgtatagcct atatagtagg atccatcacg acactccaga acgtacatat aagccttatg
    12481 atccataata aatctcttcg atttcgggcg tataagagcc atcatcattg tggacaatca
    12541 aaggaggtaa gaccttaaag ccacttgttg agccatcctt gatcgcctca atcaaaagca
    12601 tattggcttc cttttctctt tttggataaa caaactgcag gcgcttaggg gctagattat
    12661 gtcgttttaa cgtatccaaa atatccagaa gtcgatcagg acgatgaacc atggccaaac
    12721 gcccattaga cttgagaata ctctgggcac tacgacagat ttcttccaaa ttagtcgtga
    12781 tttcgtgtcg agccaagaga taatgttcac tctcgttcag attagaataa ggatccacct
    12841 tgaaataggg tggattacac aaaatcatat ccaccttact cccctgaatg tgagcaggca
    12901 tatttttcaa atcatcgcag atgacctgca tttgctcctc taatccattc aaacggacag
    12961 agcgttcagc catatccgcc aaacgctcct gaatctcaac agacaatatc tgtgcttgag
    13021 tacgagtgct agcaaaaagc cccactgctc cattcccagc acagaaatcc acaatcaacc
    13081 ccttcttagg aaaacgtgga aatcgtgata agagaacact atccaccgaa tagctaaaaa
    13141 cctctctatt ttgaatgatt ttgatatctg tcgaaaagag ctggttaatg cgctctcctg
    13201 attttaataa ttgttcttct tccatggtcc tattatagca aattcatatt aacattacaa
    13261 aaaatataaa actctaaact acttcttctt ttttaaatgg tgcagggctt ctccagtcca
    13321 gattggtagc attcgtcgaa agggagcaaa accgtagtta aagcggtcgc ttgaaaagcg
    13381 tctccgtcta ggaaactggt acttttcttc ctccaaagtg cggatagaaa gactggcttt
    13441 ccccgtaaat tcatctaaat ccactacctg aacttgaacc tcttcatcga ctttcaaggt
    13501 ttcatgaata ttttcaataa atcctgtccg aatttctgaa atgtgaatca gccccgtatc
    13561 acccgtctct aactcaacaa aggcaccgta gggctgaatc cctgtaatac gcccctttag
    13621 cttatcaccg attttcatct tagtcctcga tttcaatagt ttcaattaca acatcttcaa
    13681 ctggcttgtc catagctcct gtctcaacag cagcaatggc atccaagaca gcgtaagatg
    13741 cttcatcagc taactgacca aaaaccgtgt gacggcggtc taggtgaggt gtcccacctt
    13801 gattggcata gatttctgca atcggttctg gccaaccacc acgagtaatt tctttcttag
    13861 aataaggtag gtgttggttt tgcacgataa agaactggct gccgttggta tttggaccag
    13921 catttgccat ggaaagagca ccacggatat tgtaaagctc ttctgagaat tcatcctcaa
    13981 aagattcgcc gtagattgac tggccaccca taccagttcc agttgggtct ccaccttgga
    14041 tcataaagtc cttgataata cggtggaaaa tgacaccatc atagtagcca tcttttgaaa
    14101 gagatacaaa gttagccact gttttaggag catgttcagg gaaaagcttg atacgtaagt
    14161 ctccgtgatt ggtcttaata gtcgcaagag gaccttctac tgtttcaatg tctacttgtg
    14221 gaaaatgcaa ttctttttct accataccaa atacttctaa ggcagcaaaa atgccatctt
    14281 cttctaatgt ttttgtaata taatctgctt tttctttgat tttatcatga gaaattccca
    14341 tggcaacgct gattccagca taatcaaaga gttccaagtc gttgagacca tctccaaaaa
    14401 ccatgacctt ctctggtttc aagccaaggt gttccacaac cttttccacc cccgtcgctt
    14461 tggagcctga aatcggcaca atatcagacg aatgttgatg ccaacgaacc atacgaagtt
    14521 tgtctgagag actgtcaggc aagtgcaagt catctccctt atcttcaaaa gtccacatct
    14581 gatagatatc ttctttttca tggaaatcgg gatctacatc taagtcggga taaattggat
    14641 tgatagcttc actcatcata tcggtgcgag tcgacaactt ggcatcatga ctcccaacca
    14701 agccatactc aattccttct tgcttagccc aagagatata ctcctcaaca tctgactttt
    14761 caatctgatg ctgataaatg acctgacctt ttttatcttc gatataagcc ccattcaaag
    14821 ttacaaaaaa gtcaggcttg agatcacgaa tctctggaac aacaccaaaa atgccacgtc
    14881 cagaggcgat tcctgttaaa attccttttt cacgcaactg tttaaaaaca gtggggattg
    14941 tagttggaat aaaccctgtc tttgaattcc gcaatgtatc atcaatatca aaaaagacaa
    15001 tcttgatctt ctttgccttg tatcttaatt tcgcgtccat ctcactacct ctttcaatct
    15061 aactctttcc attatatcat aaagtaggca aatcccctat tttcaaaaag tttatcattt
    15121 ttattttaat ttcttggatg agaaaagaga catatttatg aaaaagctcc atcgtgcttt
    15181 taatgtgttc tcttgttttc aaactcgtaa aaagggagcc actgagccta actcgctctc
    15241 tcatttcaaa gcttgtgaaa aaagacccgt tggggtctta attcgctttc ttgttttcaa
    15301 gctcatgaaa aagagaccca accgggtctt ttctttaatc ttcgtttacg aaaggcatca
    15361 aagccattac gcgagcgcgt ttgatagctg ttgttacttt acgttggttt ttagctgaag
    15421 ttcctgttac acgacgagga aggattttcc cacgttctga aacgaaacgg ctaagaagct
    15481 cagtatcttt gtaatcaaca tattcaattt tgtttgctgc gatgtaatca acttttttac
    15541 ggcgtttgaa tccgccacga cgttgttgag ccatgttttt tctcctttat aagtttagtt
    15601 gtccattaga atggtaaatc atcatctgaa atatccaatg ggtttgttgc tccaaatgga
    15661 ttttcattac gtgaaaagtc tggtactgaa tttgtaggtg ctgaatagtt tgcagttggt
    15721 gcagagtaag ctccacctgt gtgaccctca cgcacactac ggctttccaa catttggaaa
    15781 ttctcagcca cgacctctgt cacgtagaca cgttgtcctt gctggttatc gtaactacga
    15841 gtctggatac gacctgtcac cccgataagt gagccttttt tagcccagtt agcaagattt
    15901 tcagcctgtt ggcgccacat aacgacattg ataaaatcag cctcacgttc accattttga
    15961 ctcttaaatg tacggtttac tgcaagagta aaagtcgcaa ctgctacatt tgatggggta
    16021 taacgcaact cagcgtcacg tgtcatacgc cctacaagta caacattgtt aatcatagtt
    16081 taccttctta cgcgtcaatt ttgacgatca tgtgacgaag aatgtcagcg ttgatttttg
    16141 aaagacggtc aaactcttta agagctgcat cgtcatttgc ttcaacgtta acgatgtggt
    16201 aaagtccttc acggaaatct tggatttcgt atgcaagacg acgtttttcc caagtttttg
    16261 attcaacaac agttgcaccg ttgtcagtca aaatagagtc aaaacgtgct accaaagcgt
    16321 ttttagcttc ttcttcaatg tttggacgaa tgatataaag aatttcgtat ttagccattg
    16381 atatgttcct ccttttggtc taatgacccc aagactttgc aaggggtaag tgaggttcgc
    16441 tcacaataaa ctattatact agaaaaaatt tttttacgca agtaaaaaca ctagaattcg
    16501 aaaaaacgcc acatgggcgt tttcctgttc ttatggtttg atacggtgca acatacgtgg
    16561 gaatggaata gcttcacgga tatgttttgt tcctgctgca aaggttacca tacgttcgat
    16621 accgatacca aatcctccgt gtggaactgt accgtattta cgaaggtcaa ggtagaattc
    16681 atattctgta cgatccatac caagttcatc catcttagcg acaagggcat cgtaatcttc
    16741 ctcacgcata gacccaccga taatttctcc atagccttct ggagcaagca agtctgcaca
    16801 aagcacgcgc tctggatttc caggaactgg tttcatgtag aaggccttga tggctgctgg
    16861 atagttcatg acaaatgttg gcacaccaaa gtggtttgaa atccaagttt cgtgtggtga
    16921 cccaaagtca tcaccatgct caagatgctc gtagtcagca tcttcatcat tttcatgctc
    16981 ttgcaagagg tcaatggctt gatcgtaagt gatacgtttg aatggctctg caatgtagcg
    17041 tttcaagagt tctgtatcac gttccaaggt ttccaaggct tgaggcgcgc ggtcaagaac
    17101 accttgtaga agagctttca cataagcttc ttgcaagtca agcgactcat catgtgtcaa
    17161 gtatgagtac tcagcatcca tcatccagaa ctcagtcaag tgacggcgtg tttttgattt
    17221 ttcagcacgg aaaactggac caaagtcaaa gacacgacca agagccatag cccctgcttc
    17281 taggtaaagt tgacctgatt ggctcaagta ggctggcgtt ccgaagtagt cagtttcaaa
    17341 gagttctgta gaatcttctg ccgcatttcc tgaaagaatt gggctgtcaa acttcataaa
    17401 accgttcttg tcaaagaact cataagttgc acagataata gcgttacgga tttgcaacac
    17461 agctacttgc ttacgagagc gtagccacaa gtgacggtta tccatcaaaa agtctgttcc
    17521 gtgttctttt ggtgtgattg ggtagtcttg agattcaccg atcacttcga tgtctgtgat
    17581 gtccaactca tagccaaatt tagaacgttc gtcctctttg acaatacctg tcacataaac
    17641 agacgtttct tggctcaagc gtttgataac atcaaacttc tcaagtccca cttcttcacc
    17701 aaatttttcg acaaagtttg gtttaaaagc cacaccttga aagaaggctg ttccatcacg
    17761 caattgtaag aaagcgattt tcccttttcc tgatttattg gcaacccaag cgccaatcgt
    17821 cacttcctga ccaacatagt cttttacgtc aataatcgtt acacgttttg tcattatttt
    17881 tccttttctt ttttattctt tatggcaaac cacctctata ttgttcccat ccaggtcaat
    17941 cataaaagca gcatagtaaa tcggacgctc acttcgataa ccaggagccc cattgtctcg
    18001 cccacctgcc tctaagccag cctcataaca agcctaaact tcttccttat tttctgctaa
    18061 aaaagcaaaa tgaacaggat cttgtgttcc ctgagtcagc caaaaatcac caccaggatg
    18121 agggctgttc ggggatagaa aactaattag agaactagtc ttaaaagcca atttatagtc
    18181 caaaggagcg agaaaactcc tataaaatcc ttctgaaatt tgtaaatcct ttaccttaat
    18241 ctcaaaatga tcaatcattc tcactaccca taaatgcttt caagcgttcg actgcttctt
    18301 taagcgtgtc taggtctgtc gcatagctga ggcggacatt ttctggtgct ccaaatccag
    18361 ctcctgttac caaggccact tcggcttctt ctaagatagc agttgtaaag tctgtcacat
    18421 ccgtgtagcc tttcatctcc atggcctttt tgacatttgg gaagagatag aaggcccctt
    18481 gtggtttgac cacttcaaat cctggcacct ctgcaaggag gggatagatg gtattaagac
    18541 gtttctcaaa ggcctgacgc atgctttcta cagtatcttg ctcacctgat agagcctcaa
    18601 ctgctgcata ttgggctact gctgacggat tcgaagttgt ttgacctgca atcttggaca
    18661 tggcagcgat aatgtctgct tctccaacgg cataaccaat ccgccaacca gtcatggcat
    18721 aggttttaga cacaccattg atgaccactg tttgcttgcg aatcgcttcc gataggctag
    18781 aaatcggtgt gaactcatga ccattataaa ccaagcggcc atagatatca tctgctagga
    18841 tgagaatatc attttctaca gcccagtttc caattgccaa gagttcctca cgggtgtaaa
    18901 tcatacctgt gggattagat ggcgaattca gcaccaaaac cttggtcttg tcagtgcgag
    18961 ctgcttctaa ctgctctacg gtcaccttaa agtgattgtc ttccttagca gaaacaaaga
    19021 cgggaacgcc ttctgccatc ttgacctgat ctccatagct aacccagtat ggggttggga
    19081 tgatgacttc atcacctgga ttgaccacag ccataaagaa ggtatagaga gaatatttgg
    19141 ctcccgcagc gactgtcact tgatttgacg ctacagaata gccgtaaaag cgctcaaagt
    19201 agctattgac caccgcctta agctctggca gacctgaggt tactgtataa aaagaagcac
    19261 gcccatctcg aatcgatgca atggcggcat cttggatatt tttgggagta gtgaaatctg
    19321 gctcacccaa ggttagagac aaaatatctc tgccctcagc cttcagtgct ttggcacggg
    19381 ctccagcagc caaagtcaca ctttcttcca tttctaaaac acggttggat aatttcatag
    19441 gccctccttg ttgaccaatg ctcctgtttc aaaatctact agataaaaat cagatcctga
    19501 cttgacttcc cagattggct tatcttgata acgaccaaag ataatcttgt caatttcgcc
    19561 agctcccttt tccttagaaa ccgtttctgc tttttcttgt gaaacaccct gatttagctg
    19621 ataaacgtaa atcttatgat catcttttcc aatcagaaca gcaagtgctt cttgctgttt
    19681 attacgacca agaacgctgt aataagattc caagccattg tataaatcaa cctgatcggc
    19741 ctgctctaat cctgcatact gctgagctaa tttttctcct tcacttttag ctgtttgata
    19801 gggtttcatg ctaagagaaa ccatatacag aaaggaagca ctgataacca caaacaaaat
    19861 cgtcatccct agaccatact gccacagtag attatttttt gctttgtttt gtcttttttt
    19921 cactcgtcta ttttaccatc tattaagctt tattacaagt gaatataaga atactcttcg
    19981 aaaatctctt caaaccacgt cagctttatc tgcaacctca aagctgtgct ttgagcaacc
    20041 aattctattt ctcccttcaa acaaaaccga ttttgaaagt gaaacagttc ttactttttc
    20101 agtcacaaat gactagagtt tccgaaattt tacaattttc aaataaactc tgagcaaatt
    20161 tcttgcaagt tcttttgttt tgttgtaata tattttataa caacgagaga gttctcgaaa
    20221 ttttaagaaa aaggagacac atcatgtcta aaaaagtatt atttatcgtc ggatcactac
    20281 gtcaaggttc tttcaaccac caaatggcgc tcgaagctga gaaagcactt gctggtaaag
    20341 cggaagttag ctaccttgat tattcagccc ttcctctctt cagccaagat ttggaagttc
    20401 caacacatcc agctgtagct gctgctcgtg aagcagttct cgttgcggat gctatctgga
    20461 ttttctctcc agtctacaac ttctctatcc ctggtacagt gaaaaacttg cttgactggc
    20521 tatctcgtgc ccttgacttg tctgatacac gtggcgtttc tgcccttcaa gacaagtttg
    20581 tcacagtatc atctgtagcc aatgcagggc acgatcaact tttcgctatc tacaaagacc
    20641 tcttgccatt tatccgtaca caaggcgttg gtgatttcac tgctgcacgt gttaatgact
    20701 ctgcctgggc agacggaaaa ttggttcttg aagaaacagt cctaaactca cttgaaaaac
    20761 aagctcaaga cttggtcgaa gcgattcagt aaaaaataga aaattttaga aagtctaaat
    20821 caattatagc cacctaatag ggtggctttt tatttctcaa tcaagatttt cagacaaaca
    20881 tcagccatcc aaaaaaacct tgagcgttac cactcaaggt ttttagcagt cttttccatc
    20941 ataggaaaat cattctaaga atattctttc attcgagatc tatcacttat catcttcttt
    21001 cgagaaatta gtgactgctt ttggctgatt aatcttcttt tcttcgtcct acatataggc
    21061 tcaatagaat accgctacct aaaagaacag agatataatc agattggctt ccagtttcag
    21121 gtaatttgtc cccacggttt tctttacctg tagttggtgc tactgggtta gcatttactg
    21181 gagttgctgg ttgagccggt gttggtacta ctggttgttc tggaaccgct ggtactggtt
    21241 taaccggtac ttctggtgtt ggaacagctg gagttggttg tactggcact tctggtgtcg
    21301 gtacatttgg tgttggttgc gctggcacag gtttaaccgg tacttctggt gtcggcacat
    21361 ttggtgttgg ttgcgctggc acaggtttaa ccggtacttc tggtgtcggc acatttggtg
    21421 ttggaggaac tggtttgtca tttgatggtg ttactggttt gtcttggcat ccacattttc
    21481 catctttacc gtctttgccg tctcgaccat cacgtccatc tctaccatct ttgccatctt
    21541 taataactgt ttttgtcaca gttccatcag aattgatgat tgtgattgta tgactaccat
    21601 caccattatc ttcaacagaa acttttggtg atttaccatc tttaccgtct ttaatcacca
    21661 tttctgtctt acttccatct ggattagtga ttgtaacaac gtgagttcca tcgttattat
    21721 ttttaacagt tacagttggt gaaacgctat ctttgccgtc tttaccatct ttgataatag
    21781 atttgtattc acgaccatca gaatctacga ttgttaatgt atgagttcca tctccgttat
    21841 caacgatgtt cgcttttggt gatttaccat caaagattgt agttgtcgtt cctttaccat
    21901 cagcacctgt cactttaata gtgtgtgaac catcgttgtt atcaataact tcaagttttg
    21961 gttctctacc atcacgaact gtagttgttg tagttgttcc gtctgagttc acaactgtga
    22021 tagtgtgagt tccatctcca ttatccacaa ccttagcagt tggtgattta ccgtcacgaa
    22081 ctgtggtttc agaaacagta ccattaccgt tttccacacg aatagtgtaa gtaccatctt
    22141 ggttatctct aactgttgct actggtgatt taccatctgt aatgattgtt tcagtagttg
    22201 ttccgtcacc atttagaaca gagatcttat gagttccatt ttgttcatca gttatagtca
    22261 cttttggtga tttaccatca cgaactgtgg tttcagttgt cacaccttct ggatttgtaa
    22321 taacgattgt atgacttccg tctccattat cacgaacaga tgcggttgga gtacgtccgt
    22381 caacaccgtc acgtccattc ttaacaacaa actcattctt agttccgtct gggttagtga
    22441 ttgttactgt gtggcttcca tctggatttt cagtagttgt tgctgttgca gctttaccgt
    22501 ctttaccatc tttgatgata gcgttggtaa cattgccgtc tccatctgta actttaacag
    22561 tatgagttcc atcgttgtta tccgttactt caactttagg agtcttaccg tctttaccgt
    22621 ttttaacaac agtttcttta gttgaaccat ctggatttgt caccgtaatt gtgtggcttc
    22681 catctgggtt ttctgttgta gtgatattcg cagttttacc gtctttacca tctttaacaa
    22741 ctgttgttga ttcagaacca tctgggtttt gaactgtgat agtgtgagtt ccatctccat
    22801 tatcttttgt agtaatagtt ggagctgaac catcacgtcc atctataccg ttataaatat
    22861 ctttactatt taatacttta tcgacacctt cgtcgtatac cccattgtca ttaacatcat
    22921 cataaactgt aacacggata ccgttacgcg ttggagttgc agcagcatct gtagcgcgtc
    22981 taattcttga aggtgaagta atatcttcta ctttgatttt cggtgcacgg ccatctttac
    23041 cattcttacc atcttttaca gtgaaggtta ctggatcttt acctggtgtt gtgatagtta
    23101 caacagtgct ttggccatct tgtccacgtt gagtagtgat tgtaggagtt tgtccatcct
    23161 gaccggttgc accagtttct cccttagcac catcttgaac aaagactgta gttggttgtg
    23221 aaccatcatg gttatcaaca gtgatttcta cacctttatg ccctgcttca tctttacgaa
    23281 cagctgtaac tgtaggtgat tttccatcta ctccatctct aattaccgca agtggttttt
    23341 ctggatttaa tggttgtcct gggttagctg gatcttcttg gtaaacttta gtttctttac
    23401 catccttgac agtaagcaat gatttaccat ctcgtccgtc cgcgccttta gcaccatctt
    23461 taatcatttc tgaacctaga agttcatcag ttcctggtgt atatttaccg tcattgtttt
    23521 catctgtata gaatgtcaca attgtatgag atggatttcc gttttttcct tggacacgaa
    23581 gtaaatcaac ttttggagtt ttaccgttca gaccattttc tccgtcctta acatttactg
    23641 taactggatc tttacctgga acagtgaatg taatgtcagt actatggtca tctttacctg
    23701 gagttacagt tacttctgga gtcttaccat cttctccgcg ttcacctttg tcacccttag
    23761 ggcctttgat gttgccctct ttatcccagt tgccgttatt cttaacgaag acgtcgcctg
    23821 tttctgtatt aacgtattta tcgccgtctt taccttgatg tgcttctggg ttagctttgc
    23881 cgttcaacac gtctttaccg tcacgtccat cacgacctac tgcgccatcc gcacctttag
    23941 ctccttgcgc tccagttagg ccgcgttcgc cttggtctcc tttttcgcct ttagcaccat
    24001 ctgcaccttt gtccccttta gggcctttga tgttgccttc tttatcccag tttccattgt
    24061 ttttaacata gacatcaccg gtttcggtat taatatattt atctccattt ttaccttggt
    24121 ttggttgtgg atttactttt ccgtttaata catctttacc atcacgacct gcttgtcctg
    24181 tagctccatc acggatcgta gttgtcacat ctggacttcc tggattatga accgttaatg
    24241 tatgtgtgcc gttttgcccc tcagttattg ttactgttgg ggttttccca tcagcaccct
    24301 tttgtccatc ctctacaaat gttgtttgtg gacgacctcc tactggtgta attgttacta
    24361 atacaccatt tttgccattt tgcacacttc gtgtcgctgt aattgttggt gattgtccat
    24421 tagtaccgtt tcttccatca gtgatagttg ctaatggttg gtttggatta agtggttgtc
    24481 ctggtctagc cggatcctct acatagactt ttgtttcagt tccattttta acagcaatta
    24541 gagattttcc atctttacct ggagttcctg gattaccttg ttctcctttt ggtccttgtg
    24601 gtcccgctgg tcctctttgt ccatctgcgc cttttggtcc ttgagctcca tcacgacctt
    24661 gaagaccttg ttcacctttg gcacctttgg caccttgcgc tccagttaaa ccgcgttcac
    24721 cttggtctcc tttttcacct ttagatccag ttagaccgcg ttcaccctga tctccctttt
    24781 cacccttgac accttgcgct ccagtcaggc cgcgttctcc ttgggctcct ttttcaccct
    24841 tggcaccatc tgcacctttg tcacctttcg gtcctttgat gtttcctgct ggttcccaag
    24901 tgttaccttt cttaacgaat acatctcctg tttgtgtatt tacgaatgtg tctccatctt
    24961 taccttctgt tgttgggtct acttttccgt ttaatacttc ttttccatca cgtccagatg
    25021 cgcctgcagc tccgtcttgt ccgtctttaa ctactgttgt ttgaactaac tcgtcttttc
    25081 ctgctgtgta acttccatct ccatctttgt caatgtagaa tgttaatgtt gtttgtttct
    25141 tcgcatcatc tcgttctgct ttgacttttg gtgtttttcc atcttcaccg tttttaacga
    25201 ttgttgttaa tgatggtctg ttgtctggtt gtgtgattgt gatggtgtgt gttccgttat
    25261 tgttatctgt aactgttact tctggtgtga atccatcttt tccgttatca cctttgtcac
    25321 cttttggtcc ttgttttcca tctgcaatta caacttcttt aagttttgta tctccttcat
    25381 caaattgatt attatcattt agatcataat agaaaataac agttgttgtt ttatctgctt
    25441 catttctatg agtagaaaca agagggcttg caccatcaaa tccatctctt actactgtag
    25501 tagttatacc acctttacca tcgttaattg taattgtatg cgttccatct tgaccatcag
    25561 ttactttaac tgttggggtt ttcccatctt ctccttttgg tccttgttca ccatcacgcc
    25621 cttgaagacc ttgttcacct ttgtcccctt tttcaccttt agcaccatct gcaattacaa
    25681 cttcttttaa tttcttatca gaagcatcta attcattgtt tccattttta tcgtaataga
    25741 agataacagt tgttgtctta tctgcgtcat ttcgttgagt cgcaactaat ggacttgcgc
    25801 catcgaaacc atctcttaca acagtacttg taacattacc tctaccgtcg ttaatagtga
    25861 tagtatgagt gccgtcattt ttattatctt taacggttac ggttggagtt acaccgtcac
    25921 gacctgcctg accttgggct ccagtgagac cgcgttcacc ttggtcccct ttttcacctt
    25981 tagcaccttg tgctccggtt agaccgcgtt caccttgggc tcctttttca cctttagctc
    26041 cttgagctcc agttagaccg cgttcacctt ggtctccttt ttcaccttta gctccatctt
    26101 taatagcaat tttttgaaca agttcgtcta caccttcagt ataaacaccg tttccatcgt
    26161 tatcaagata gaaagtgagg ttatttgtat caccgacttt attttgatca acacgtggtt
    26221 ttactgtatc gattagatct ttagccacac caatttgcat aatacgtggc ttagcttctt
    26281 tagtaacact attactaatc ggcaatccta aaagtgtttc gttaccaaat cgatgaactt
    26341 tataggtaat ttgacgttgt cctttttcac cttctcgaac taatctagtt tgaccttctg
    26401 gtaacttact agttggaaga tagacagttt ggaagtcaat gtcttctgaa acagtcttat
    26461 tattagtacc accataaggg tttgcatcca agtatagtgg gtttgctcta taataatagt
    26521 tagagttact gtatgggtca ttgctagtag cggcaccacc accaccttgg tccacgttaa
    26581 aagtaacagg tttcaaacga acagacaaat caccatttag attaatgata ggcaaactaa
    26641 atctgatagt ttgttccgta tttgttgaaa tagcagaggt catactgtat cctgagtgag
    26701 atacgtagtt ggcaccctga ctaatagatt ttgattgctc ccttactctt ggatttgaac
    26761 ttgtgatcgt tgcatttgaa ggatttccaa atccatcccc tttatccaag ataaaattat
    26821 tattaacaaa ctctctagaa tctcctggcg cagaataact aattgtaaag ttaacactat
    26881 taccactacg tactgttgtt acatcgtaat gaatagaatt tccaccacgt gagttcccgt
    26941 agccacggaa actatagcta cgaccacctt ggctaatatc tgcaaatgca actgtatttc
    27001 cagaacctga gacaattggg ttggctggat tgctagccgc tctaaaacca ctataccctc
    27061 tattaacatc ttccttacgg atagctggat tttggcgagc atcagaaagg ctcaaaccta
    27121 gtttcaagat ttcattaata gatgctggag ctgaagttgt ttctactttg tcagctgtgc
    27181 tagttggcaa aatagcgcta tcaacagctg aacgttcagt tgttgcagct ggtttagccc
    27241 cttcttttga ttcaactgca tcagctttct tcgctacagg cgtttctgat acagtttctt
    27301 tattagagtt agaacgaagt tctagaccaa taataaatga ttgaatagat gactctgctg
    27361 ctgataattc agcttcagtt gcatctgctt ttccaagcaa gctttgacca gcttggatac
    27421 ttgtttgcaa gttagcagca cttgcttctg tatataactt gctttctaat tttttagcac
    27481 gcgaaagagc cgttaataat tttgacttgt caacggcatc ttgatgcgtt acttcctctt
    27541 ttttgtcttt tgcttcttca actttttctg ctggtttagt aacttcttca gttttaactg
    27601 gagtcgtttc tactggatta gctacagttg gagctgcata ggttttctct ttattagacg
    27661 cagaaacctc tgctggttta gtagctgctt ctcccctacc aacagaaata ctattggtag
    27721 tttcttgttt gttttcttcc gcttgtactg tttggacact agctgctgca cctaatacca
    27781 aagcggtacc gagcaagaca ctagctgcac caaagtaata cttacgaata gaataacgtt
    27841 gttgcatgct ataccagtca aatgattttt tatgtgaatg tttcatttta aactcctaga
    27901 tagtatttta tagtttacaa tgtacaacta tttgaggcat taatcaatat tttctgaaca
    27961 attaatatat tctgtttttg cacttaaaag ccacttatat tattctaatt tttccgatta
    28021 ctaacgacaa ttttcttgct ctaatttttt tatactactt agacaaaaaa agatataatg
    28081 aattatggag gaaatacccg tatgagaaac cttttatcca caaaagttca aagacaatta
    28141 cgcttaatgg aaaccctaat tcagaatcgt aattggatga aattacatga actagccgaa
    28201 aaattaggat gtacagaacg aatcttaaaa agtgatttaa atgaattacg tattgccttt
    28261 ccatctatta acatccaatc ttctgttaac ggaatcatga tagatttaga agttaacact
    28321 agtgtagaag atatttatca atactttctt gctaattctc aatcttttca attactagag
    28381 tatatgttct tcaatgaggg gctacctatt tatcgaacaa tagaaaatct ttactttagt
    28441 agtgccaacc tctatcgact gggcagaaat attacaaagg ttctttcatc tcagtttcaa
    28501 attgaattat cttttactcc atccgagata cgtggaaatg agatcgatat tcgttacttt
    28561 tttgcccaat acttttctga gcgctattat ttcctagatt ggccttttcc tgaccttcct
    28621 gaggaagatt taacagagtt tgctgacttc ttctataaaa tcacaaatta tccaatgcga
    28681 ttttcaattt atagaatgta caaactgatg atagctatca gtatccacag ggttaaaaat
    28741 ggtcacttca ttgacctccc caaccacttt tacaaagaat attatcctct tttaaagagc
    28801 attccgaatt tccaggagac acttgcttat ttctctaaac attttgggct tgaaatgact
    28861 ccagatacaa tcgctcaaat ttttatttct tttcttcaga atgatatttt cctagatcct
    28921 caagaattct tcaactcttt agaagataac agtcaagcaa gatactctta ccaattactt
    28981 agtcaaatat tagaaggcct atcaaaacaa tataagatta cttttactaa ccacgatgag
    29041 ctgatttggc atttacacaa cactgctttc tttgaaagac aggaaatctt ttctacccca
    29101 atcttatttg aacaaaaagc attgacgatt aaaaaatttg aagtttactt cccagacttt
    29161 atgggaagtg cgcgtcaaga attggctcaa taccgtcagg caattggaca gcatgaccat
    29221 cctgaacagt tggaacactt gatgtacacc atcttaaccc atgctgaaaa cctctctact
    29281 cagttgttag aaaatcgacc acctatcaag gttttgatta tcagtaactt tgaccatgct
    29341 atatccctta cctttgttga tatgctttcc tattactgta ataaccgctt taccttcgat
    29401 atttgggatg aattaaaaac aagtcctgag attttaaatc agacagatta cgatatcatc
    29461 gtgtctaatt tctatattcc agggattacc aagaagttta tttgccgaaa tcatctgtca
    29521 atcatgaatt tggttaatca tcttaacacc ttatcaaatg agattcattt gtccaatact
    29581 ttataagtta tcaaaaaagt cagcataatc gtgctgactt ttctcttatc tgatttctat
    29641 acttccttta caaaaaccaa tccctgtggt tgtgacaaat cttctcggta aacaaatcca
    29701 gcaaagttca agcgacgtgc ttcccccaca gtttgtactt gattttctat taaagctgtt
    29761 agaaaggccc cgcgcgcttt cttggagata gttgagtgaa tcttcagctg accgcctcta
    29821 tcctccatga atttgaaggt caccatcttt gctctgattt ccttagaaaa tacagtctca
    29881 aactctgatg acaagagaga gaaaatcact tcttccttct tcagagcttc atcatagact
    29941 gccttccaat ggctcttcaa agtcttacca gcgactttta atttcatcaa aaaatccaaa
    30001 cggtgaggag ccatgggtga caaggctgga acaacaccgt acaaagccga ggtaatgaag
    30061 acatgatttt caagataatc ttgttccgcc tcggtcagct tatctctctt aatgttgcgg
    30121 tacataagcc catcaaaaag tttcaaggct ggatagtgtt gagcagtttg ccttttcaaa
    30181 gcttggatat tttgaaattc ttccgccgct ttctcagctg ataccttgta gaaactctcc
    30241 aattgactgg cagaatagag agccaaggca tcaagcacgg tctgactttc tggttttaaa
    30301 ggaattgcct cgatacttgg caagtctgtg ttcatttctt ttgctgttgg gattaaaatt
    30361 ttcatattga tagtgtagca tattttttag ccacaaccaa gaaattcatt tgaaaaacct
    30421 cggtttgacc gaggtttttc tatttatttg ggccatccta accagacagc cgcgagaaca
    30481 agggctatgc cagctaaact tgttaagata gataagaatt tatctttttt ctccttgaac
    30541 tgagaaaaac ctatcttcaa agcgagcagt ccgagtagca ttgaagcaac catcaaataa
    30601 aaacccattt ctttgtcctc cattaggctt aatttacctt attataacat atatttctta
    30661 aataaacctt ttttatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc
    30721 tgctcaaaac actgttttga ggttgcagat agagctgacg tagtttgaag agattttcga
    30781 agagtattat tgtactttaa aggtcttgag ataattgtct tttcctactt gaccttcgaa
    30841 ggttttacca ttttcaaggt aaggaaggtc atcggatact gaagccttga ccttgtatat
    30901 cttgccatca actttaaaga agtagacagt gtctcccttg ataacagctg atttgaggtc
    30961 tgctactact cccttgatgc tttctgtcgt tgcattgtca atttcaaggt cgtttttatt
    31021 ggcatacttg ctgagcatct cttccactgt agtagcaacg ataacatttt ggtactcgac
    31081 tgcgtctacc agggcgtact ctttgaccaa gccagcattg tcctttaagc ccatgatgta
    31141 gagaggcttg tcattgaggt tgatgaggat tgggaaggtt gctttgtagg atttctcctg
    31201 aacagcacct tctgctgatt cacgggctga ttcttctgtc gcagaagcca agctatactt
    31261 agtaatttct cctgttcgca tattttcaag gatgaaacca agattactct catccgcatt
    31321 agccgacgtc acacctgtgt agagatagat gtcattaccg atagacaagt aattatagcc
    31381 attggtagtc tgggtcacgt ttttcttgga aatcatggca ttcaagaaac cgtccttgta
    31441 cttgccgttg tagttgattt gctcaatggt ttcctctgct ggatagatcc tgtccaccca
    31501 ttctggaaca tctgataagc tgtattcctt ggtttctcca tttgtagcat ccaagataat
    31561 gactgaagca ggacgaggaa ccgcaagtcc aaattgcttt tggtaaaccg ttgctacata
    31621 gaaaggattg ccctcatcgt ccacctcaaa agatggagtt ttaaagattt tagtcgggta
    31681 cttcaagcgc aggtgacgtt tgacatcacg gttaaaatac tccgagtctg aatacttgat
    31741 tggtgtcttc aagtccacca aatccgcatt tccagttacc atgtccacct tgatatactc
    31801 accgattccc ttggcttgat tgttaaacca tttgataggg tctgcatatt ctagtggtgt
    31861 gacccgataa ggtttcccat caattgtcaa ttgggtatag gtatctgccg ctacgtattg
    31921 cgacacctta tcggttaggg aacccaagta gcggtctcca attttttcag cagtacttct
    31981 atctaggata ggaaccttac tggtgtcact cctaggaaat tcagtaaagt ctttttccgt
    32041 aaccgtgact acattggcat aatttttagc ctgaaacatg ctggaagtta ccaaggaaac
    32101 caaagctgcc aagagaagaa ttcctccaat cgaagctaac agaattttcc ctaaccgatt
    32161 gattttgaaa ccctcaagat ttaaggcagc ttccgcctta ccgtggcgca catgaaccgt
    32221 tttgacaagg tttatcccct tgccaaagcc aaataagatt gccacaacta acaaatgccc
    32281 acagaggaag aagagaaatt cccagctggt caggttaagg ggcggtaaaa agatatacca
    32341 ggtcgttgcg ataaaaataa gttcacagac tattcgtttc atttgctttc ctcctaaatg
    32401 attcgtcctt ccaagtgatc cagctcatgc tggcaaatct gagctgggaa gcctgtcaag
    32461 gtaatggtct gttcctgcca cttgctgtca cgataggcaa gccttgtggt ttcataacgc
    32521 ttagttgatc tcacacctac caaggacaaa cagccttctt ctgcctcata ggatccttca
    32581 aaggacagaa gcactgggtt aaacatgacc acgggaacca agccaagatt aaagataatc
    32641 acgcgcttct gcaccccaat catattggca gctagaccaa cacaggtatc acgatttgct
    32701 aagagtgtat cctgcaaatc tctggcaaga taaaggtcct cctgacttgc cggctgagac
    32761 acttgagata aaaataagat atccttcaca attttctttt ccacttcctc acactcctct
    32821 tgctttttac tatattatag caattatagc acaaaagccg ataactgcaa accctttccc
    32881 tcaactgtag caaaaagcca tccgaagatg acttttttgc tatttaattt ctgtataagt
    32941 tacttccaag ccacgcttaa cagctggacg attggcaatt ttttctgccc attttactag
    33001 attttgataa cttgaggcat ccaagaattt tgcagaacct tggtaaagat ttccttgaac
    33061 taactgtcca taccaagacc agatagcaat atctgcaatc gtatagtcat tgcctgcaat
    33121 ataaggtttc tgagccaatt ccttatccaa taaatccaac tggcgtttca cttccatcgt
    33181 aaaacggtta ataggatatt ccaatttttc aggagcataa ttgaagaaat gtccaaatcc
    33241 cccacctaga aaaggtgctg cacctgcttg ccagaatagc caattcaaaa cttctacctt
    33301 ttccacagga ttacttggta aaaaggctcc aaatttctca gcaaggtaaa gaagaatatg
    33361 agcagactca aagactctta catcttcagt acctgactgg tccaataagg ctggaatctt
    33421 ggaatttgga ttgagcttca caaagtctga tccgaattga tccccatcca tgatagcaat
    33481 cttatacaag tcgtaagccg cttccttaaa accagcttct agtaattctt ccaataagat
    33541 agtaaccttc acaccatttg gtgttcccag tgaataaagc tgaaaagctt gttctccttt
    33601 tggcaagttt tgttcgaaac gggcacctgc tgttggtctg tttagccccg taaaagctcc
    33661 ttgattacta gcttcatcct gccatacggt cggtaattga tatgctgaca tccgagacct
    33721 cccttaaatc gcattcttgt caaaaccgag tttgcgttga ataaacttaa cgatttcgac
    33781 gatgataatc attgagaagc ttccagccat aacaattccc cattgtgaca agtctagttt
    33841 ggttacgtgg aagattcctt caagcggttc tacaacgatt gttgccatga gaaggataaa
    33901 ggataccaag atggaccagt taaaggtctt agacttgaat gggccaactg tcaagatgga
    33961 ttggtagaca gacttgacat tgtaggcatg gaagagctga atcaaaccaa gggttgcaaa
    34021 ggccatcgtt agggcatctg catgaatagc atgattgtca cccacatgaa ctgggtaagc
    34081 aatcgcaagg ccataaacac tcataacaag agctgcttgg agtacacctt gataaatgat
    34141 agaactcaaa acaccacctg agaagaagct tgccttgcgt ccacgtggtt tatgattcat
    34201 gacaccaggt tccgcaggtt caacaccaag agcgatagct gggaaggtat ccgttaccaa
    34261 gttgatccac aaaagatgaa ccggctgtaa gacatcccaa ccaaacaagg ttgataggaa
    34321 gatggttaat acttcagcag tattagcaga aagtaggtac tgaatagtct tttgaatgtt
    34381 tgagaagacc ttacgtcctt cttccactgc gacgataata gtcgcaaagt tatcatctgc
    34441 aagaatcata tcagaagccc ccttagaaac ctctgtacca gtgattccca taccgatacc
    34501 gatatcggct gttttcagag ctggcgcgtc attgacaccg tcacctgtca tggcaacgac
    34561 tttaccttgt ttttgccaag ccttgacgat acgaaccttg tgttctggag acacacgggc
    34621 ataaacagag tattgaccaa caactttttc aaattcttca tctgacagtt cattgagttc
    34681 agcaccagtt aaaacgtgac cttctgtatc gtttgcgtca atgattccca aacgtttggc
    34741 aatggcttcc gctgtgtctt ggtggtcacc tgtaatcata attggacgga ttcccgcttc
    34801 cttagccaca cgaacagcct cagcggcttc aggacgttca gggtcaatca tcccaatcaa
    34861 accagtaaaa attaaatcat tttcaagctc ttcagaagtg agattttctg gaatgctgtc
    34921 aataatctta taagcacctg caaggacacg caaggcttga tgagccattt cagagttgtt
    34981 tgtatgaatg agatttgtaa ccttctcatc aatcggagca atatccccag ccttatcacg
    35041 aagaagacaa cgttttaaga gttggtctgg cgcacccttg actgctacaa ggaaacgacc
    35101 atctggcaat gggtgaactg ttgacatgag cttacggtca gagtcaaatg gcaattcagc
    35161 tacacgagga tatttctcta agaaaccttt gacatcatag cccttgtcca aggcatattg
    35221 gataaaggct gtttcggttg ggtcaccaat caagttacct tccacatcga ttttcgtatc
    35281 attggccaag acaactgaac gaagtagtgg catttcaaga cctagttcaa tatcatcagc
    35341 tgagtcatgt agaaccgcat cgtagaagac tttttcgact gtcatcttgt tcatagtcag
    35401 cgtaccagtc ttatcagaag cgatgatttc agttgaacca agtgtttcaa ctgctggcaa
    35461 cttacgaacg atagaatgtc gtttggccaa aacttgagta ccaagagaaa gaacgatggt
    35521 aacgatagca ggaagtcctt ctggaatggc tgcaacggca agggcaacag aagtcaacaa
    35581 ctcaccaagt ggatttttcc cttgaatgaa gacacccact acaaaagtaa caagggcaat
    35641 gaccaagata gcataggtca agaccttaga aaggttgttc aaattttgtt tgagtggtgt
    35701 atcagtctca tccgcatctt gaagcatacc agcaatatga ccaacttcag tgtacatacc
    35761 tgtattgaca acaacaccca tcccacgacc ataggttacg tttgagtttt ggaaggccat
    35821 gttgacacgg tcaccaatac cagcatctgt cgcaagctcg actgacaagt ctttttcgac
    35881 tggtacagat tcacctgtca aggctgcttc ttcaatttta agagagttgg cttctatcaa
    35941 acgtaggtcc gctggtacca cgtcacctgc ttcaagggca acgatatcgc ctggtaccaa
    36001 ttctttagag tcaatctctg ccatgtgtcc atcacgaaga acgcgggcaa ctggactaga
    36061 catggatttg agggcttcaa tagcttcttc agcttttcct tcttggtaaa caccaaaggc
    36121 agcgttgatg ataaccacag ctaggatgat aatggcatct gcgatatctt ccccaccaga
    36181 agtcacgact gacaagattg ctgccgcaac taggatgata atcatcaaat ccttaaattg
    36241 ctcgatgaat ttgaccaaga ttgatcgttt ctcgccttct tcgagttcat tgtgcccaaa
    36301 ttcggcaagg cgcttttccg cctcacttga tgacaaacct tgctcggtcg catccacagc
    36361 ctgcaagacc tcttcagggc tctgagtata aaacgcttgg cgtttttgtt cttttgacat
    36421 gtgtctcctc cttgacattg tgtgcaaaac agactctctt tctgtcatat cttttcacga
    36481 caaacaaaaa gagacctgtt aatcataaca agtctcgctg tttaagatag ggccggaaag
    36541 catacttttc agcataaaat tcggaatgac gacactatca caggtttctg ccagctactc
    36601 ccttgagtag taccattata ccaaattttg gggagttttc aaagagtaaa aactgcctta
    36661 tttgaatttt tccttgaaaa ccagtataat ggtagaatgc tatgtgacta gaaaggaagt
    36721 tgaatgaagc aatctatctc aaatctcaag ttagctgagc gtggagccat tatcagtatt
    36781 tcgacctatt tgatcttgtc tgcagccaaa ttagcagctg gtcatctcct tcattcatcc
    36841 agtttggtgg ccgatggttt taataacgta tcggacatca ttggaaatgt ggccctctta
    36901 atcgggattc ggatggcgcg ccagcctgca gaccgtgacc accgttttgg tcattggaag
    36961 attgaagatt tggcaagctt gatcacttct atcatcatgt tctatgtcgg tttcgatgtt
    37021 ctaagagata ccattcaaaa gattctcagt cgggaagaaa cggtcattga tcctcttggt
    37081 gcaactctag gaatcatttc tgcagcgatt atgtttgtgg tctatctcta caatactcgc
    37141 ctcagtaaga aatccaactc caaggcgctg aaggcagctg ctaaggacaa tctttctgac
    37201 gctgttacct cacttggaac cgccattgcc atcctagcta gtagtttcaa ttatccgatt
    37261 gtggataaac tggttgctat catcatcact ttctttatct tgaagactgc ctatgatatc
    37321 ttcatcgagt cttcctttag tctttcagat ggctttgacg accgcctgct cgaggactac
    37381 caaaaggcta tcatggaaat tcccaaaatc agcaaggtca aatcgcaaag aggtcgcacc
    37441 tacggtagca acatctacct ggatattaca ctagagatga atcctgactt gtctgttttt
    37501 gaaagccatg aaatcgcgga tcaggtcgag tctatgctgg agaatcgttt tggcgtcttt
    37561 gataccgatg tccatatcga accagcacct atccctgagg atgaaatttt agacaatgtc
    37621 tataaaaaat tgcttatgcg tgaacaattg atcgaccaag gaaaccaact agaagaactc
    37681 ttgactgatg attttgtcta tattcgccaa gatggagagc agatggataa agaggcttat
    37741 aagaccaaaa aagagttaaa ttctgctatc aaggacattc aaattacttc catcagtcaa
    37801 aaaaccaaac ttatctgcta tgagttagat ggtatcatcc ataccagtat ctggcgtcgc
    37861 cacgaaacct ggcaaaatat ctttcatcaa gaaaccaaaa aagaatagag aaatcctttc
    37921 atgagacggg atttttctat tcgtttatac tcaataaaaa tcaaagtgca aattaggaag
    37981 ccggtcacag gctgtacttg agtcggcaat gtgaagccga catagtttgc actttgattt
    38041 tcgaatagtc ttaactatca aattcactga gatactcata gcgttcgtat ttttcaagga
    38101 gtgcttcatt tttctcatcc aattcttttt ggagagtagc cagcttacca aagtcagagc
    38161 cgttagcctg catttcctct tcaatagcag cgatacgttt ttccaaggtt tcaatatcac
    38221 cttcaatact tgcccactcc tgcttttctt ggtaggtcat gcgtttcttg tcttctcgaa
    38281 ccttgaccac tttttccttt tcgacctttt gcacttgatt gaccatatct gtttcaaaag
    38341 ctttttcatc aagatagtcg gtgtaatgac caaagaaagg acgaatcttg ccatcctcaa
    38401 aagcgagaat cttggtcgct accttatcca agaaatagcg gtcgtgactg actgttaaaa
    38461 cgggacctgc aaaaccttgc aagaaattct ctaagactgt caaagttgca atatctaggt
    38521 cattggttgg ctcgtctaaa agaagaacat ttggtttttc caaaagcagt ttgaggagat
    38581 aaagacgttt tttctcaccc cctgacaatt tctcaatcaa ggttccatgc gtcgaacgtg
    38641 ggaaaaggaa ctgctccagc aactctgcaa tcgaagtcgt agaaccgcca ctggtcttga
    38701 cctcctctgc cacttcctgc aggtaattga taactctctt gctttcatcc aaaccctcaa
    38761 tttgctgaga gaaataggcg atgcgaacag tttccccaat cacaacttgt cctttagttg
    38821 gctcaagact tcctgcaatc aagttgagca gggttgattt tccaacacca ttgtccccaa
    38881 cgattccgat acggtctttg gcctgcacca agagattaaa atcttgcaaa ataggcttgt
    38941 tttcataggc aaaggaaaca gcctgaaact cgatgacttt cttcccaatc cgactggttt
    39001 caaagttcat ggtcaagtct gtctcagcac tactgcctga aacttccttt ttcagatcat
    39061 ggaatcgatt gatacgggcc tgctgcttgg tcgcacgcgc ctgcggttgt ctgcgcatcc
    39121 aggccaattc ttgtttgtag agttgttctt ttttgtgaag aagagccgcg tcgcgctcat
    39181 cctgttccgc ctttaggcga acatagtcct ggtaatttcc ctggtactcg gtcaagcctg
    39241 cacgatccaa ctcgaaaatc cgtgttgaca aagcgtctaa gaaataacga tcgtgagtga
    39301 taaaaaggac ggtcttctta gaatttttca aaaagagggt cagccactca ataatcgcaa
    39361 tatccagatg gttggtcggc tcatccaaaa gcaagaggtc gtggttgcca agtaagactt
    39421 gtgccaactg tacccgtctt ctcagaccac ctgacaattc cccaacagga gtagataagt
    39481 cttgaatgcc caatttgcta agaacggtct tgacctgact ttcgatttcc caagcttgga
    39541 gagagtccat ctctgccatg acacgttcca aacgcgcctg cttgtcctca ctatagtcga
    39601 gcataatcaa ttcatactca cgaatgagct ggatttcctt gagttcacta gatagaaccg
    39661 tatccaaaac tgtctttcta tcatcaaaat caggatcctg agtcaagtaa ccaatctggt
    39721 aatcattttt agctgaaaaa ggactgacat ccccatcaaa tccagaaaca ccagaaagga
    39781 cgtccaaaag ggtggtcttg ccagtcccat tgacaccgat taaaccaatt ctgtctaagt
    39841 catggataat aaaggaaata tccctaaaaa cggtcttgtc accaacggat ttacttagtt
    39901 tttcaacgat aaaatcactc attttttctc cctcaggtaa gcatggatgg cttcacgatt
    39961 attctccaat tctccatcga caatggcaaa ctcaatctct gttaaaatct ctcccaagtc
    40021 tgggcctggc tgatagccat attccttgat caaaataccg ccattaatct gaatctcttt
    40081 cttgtcatgg atagtcaagc tttggtattt ttctgtgatg gcttgtgggt tgacttcttt
    40141 tccttgagct tgacgaagat tttcagcctg taaaagcaaa tctatgtcaa agcgataaca
    40201 atctcgcttg ctcaattctc cattttcacg cagagccaaa ataatcagca aatcctgaac
    40261 ttgcttggca aactggcgtg aggtcttcca agatttcaaa aatgactgcg cattttcaat
    40321 ctccaaagcc catagtaaag ccgcccaggc ttgttcagag gattcaaaag taaaatcagt
    40381 ctccaaatca aacagtctgt tgagcttgtc ctggctagat gccatatcag ggagatagtc
    40441 ataagcttga ctctcaatca tggaagccaa gccccttctc caaaatggag ccagcaagag
    40501 tttatcaaac tcgacgaagg tacgctctac agaaattttc tccaaaagcg gcgtcaaggt
    40561 cttcatagct ttaaatgttt ctggttcaag tgcaaaacca agactagcct gaaaacggaa
    40621 accacgcata atccgtaaag catcttcgtt gaaacgctca ctagccactc caactgctcg
    40681 caagacttgc ttttccaaat cttctaaacc atggaacaag tcaacgattt ctcctgtctc
    40741 atccaaggca aaggcgttga ctgtgaaatc acggcgtttg aggtcttctt ctagcgagcg
    40801 cacaaaggaa accgcactgg gtctgcgata gtccacatag acatcctctg tccgaaaggt
    40861 tgttacctca tactcctcat ccccatctaa gaccaagacg gttccatgct cgattccgat
    40921 atcggctgtt cgcggaaaaa tctgcttggt ctcttctgga taagaagacg tcgcaatatc
    40981 cacatcgtgg atagggttat ggagaagggc atctcgaaca gagcccccaa caaaataagc
    41041 ctcaaagcct gcttctttaa ttttttctaa tactggtaaa gccttctgaa attcagaagg
    41101 catttgcgtt aatctcataa taagtgttct aatccataga caagctcatg acgcttgaca
    41161 acttctttaa ttcccaaatt gactcctgtc atgaaggaga tgcgatcata ggagtcatga
    41221 cggagggtca acccttctcc ctgattgcca aagatgactt cctgatgggc taccaaacct
    41281 ggcaaacgaa ctgagtggat gcgcatacca tcaaagtcag caccacgagc accagcaatc
    41341 agctcttcct catctgctgc gccttgctga atggactctc gaacctctgc catcaactca
    41401 gctgttttaa tggctgttcc actcggagca tcctttttct tgtcatgatg gagctcaata
    41461 atctccacat ttgggaaata tttggcagcc tgcgtcgcaa attgcatgag taagacagca
    41521 cccaaggcaa agttaggggc aatcaggcca cccaagtctt gggcacgaga aaattctttt
    41581 agctctgcaa tttcttcact cgtgaaacca gtcgttccaa ctactggagc aaagccattt
    41641 tcaagagcaa aacgtgtatt ttcgtaggca acagctggag tagtaaaatc tacccagaca
    41701 tccgcttcaa aaccagctaa atcagcctta tccttgaaaa caggaatacc ctgccattct
    41761 gactcagact caaaaggatc caaaactgcc accaagtcca agtctggatc agtcaatacc
    41821 atctgacaag cagcctggcc catctttccc ttaaaaccgg caataattac ccgaatactc
    41881 atctctactc ctgtctaaga tacaaagtcc gtaagaacac aaagtgaaaa taggaattcc
    41941 aatcaagaag tgtctacttc ttggaagaac tatctttttc acacagggtt ccaggcgtgt
    42001 tcaattatca agatacaaag gaccttagct gcccctgaaa aatagggaat ggcactgact
    42061 ttccacgaaa ggcaagacag gcatcttttt tcaagaggca ggtagtccgt gttcaatttc
    42121 taagattaac tagcctagaa gtgccaacta aatcactgga atataaccta gagcaatact
    42181 tcctgctcct aggtgcgttc caatgacact accaaatgta gcaagtgaaa catccgaacc
    42241 caagccaaaa tcaagcaaat gctgacgcaa ttcttcagcc ttttcaggag cattcccatg
    42301 aatgacaatg acccggtatt gacctgaagc cgttgtttcc ttgataattt caattaagcg
    42361 tttggtggcc ttcttttcag tacgaacttt ttcgtaaact tcaatcacac cttgatcgtt
    42421 aaaataaagg attggcttaa tgctaagcaa attgcccaaa atggcagccc catttgaaag
    42481 gcgtccacct tttaccaaat gatccaagtc atctaccatg ataaaggctg acgtacggct
    42541 gatttgaatg gctagcttat cctgaatgct ggcaaaatca tcgccctgat cacgccaatt
    42601 aaagacgctt tcaaccatga tgcctagggg agcacttgta atcaaagtgt ctgggaaagc
    42661 aatggttaag ccctcatagt catcgaccat atactggata ttttggtaaa aacctgaaat
    42721 tccagaagat aggaaaagcc ccaaggcatg tgtatagcct tgttctttga gcgaagttaa
    42781 gatttcatct aacttggcaa tacttggttg actggtctta ggcaattcag aagcctgagc
    42841 cattttttgg taaaattcct cagcagacag attgatgcct tcgacatatt cctcaccatc
    42901 aatattgaca ggaatatcca agacaaacaa gtcttctctt tgcaaggtct cttcactgag
    42961 ataagcagag gaatctgtga aaacagctaa tttcatatta gaactccaaa ttaattcctg
    43021 gtaagtctaa tgcaatttca gtcacttcgt aagtcaaacg attgagcatg ttcaaacatg
    43081 gacgagccaa ggtttccacc tcttcttggt tcaattcact tggttcattg acaatacggc
    43141 catcgatatg gtttacttgt gagattgttc cactaatgac aaacttatca aatacaatca
    43201 taaagctcaa gatgacaatc aaggaagtca cttgattttc ttggtcatgt tggagcaatt
    43261 ggaaattcac atccaccttg gtttcaggag ctccattttc attttcccat tcaaaattac
    43321 gcgcatcaaa atgatactga ctaacaaatt cttgttcacg tttaagattc atgtctttct
    43381 ccatcggcta caatattata agctattgta ccataatttt ttattttcat ctagttttct
    43441 aggatttagt caatcccaat ttcagcacga actacatctg tgatggtatc aacatagtag
    43501 tctacttctt ctgttgtagg cgcttctgcc ataacacgca agaggggttc tgttccactt
    43561 ggacgaacaa ggatacggcc gttccccgcc atttcttctt ccatcttctc gatgatggcc
    43621 ttgatagctg gcacttccat ggccttttcc ttcatgacgt tttccactcg gatattaact
    43681 aatttttgtg gataaatcgt tacttctgcc gccaactctg ataagctctt accagtttcc
    43741 ttcatgattt tagtcaattg aactgctgat aattgaccat cacctgtggt attgtaatcc
    43801 atcaagataa cgtgaccaga ctgttcacca ccaaggttgt aacctgattt tctcatttct
    43861 tcaacaacgt agcggtcacc aactgcagta actgccttgt taataccttc gcgattcaag
    43921 gccttgtgga aaccaaggtt agacataaca gttgtcacaa ttgtattttg agccaattgt
    43981 cctttttcag aaaggtattt tccgatgatg tacataatct tgtcaccatc gacgatgtca
    44041 ccattctcat caacagcaat caagcggtca ctgtctccat caaaggccaa accaatagct
    44101 gacccacttt ctttgaccac ttcttgaagg gcttctggat gtgttgaacc aacattaagg
    44161 ttgatgttaa gaccgtctgg tgtttccccg ataaccgtca attgggcacc aaggtctgca
    44221 aagatttgac gggcactggt agaagctgct ccattagctg tatccaaggc aaccttcatt
    44281 ccatcaagag gagttccagt tgaaacaagg tatccttcat acttacgcaa gccttctgga
    44341 taatctacca aaattcctaa gccttctgca cttggacgag gaagagtgtc ttcctcagca
    44401 tctagcaagg cttcaatttc tgcttctttt tcatcatcta gtttgaagcc atcaccgcca
    44461 aagaacttga ttccgttatc aagagctgga ttgtggctag cagaaatcat gacaccggca
    44521 cttgctcctt cagtttcaac caagtaagct actgctggtg ttgcaaggac accaagtttg
    44581 tatacgtgaa tccctactga aaggagacct gccaccaagg ccgattccag catttcccct
    44641 gaaatacgtg tgtcacgtcc tacaaagact ttcggcgctt ccgtttcatg ttgactaaga
    44701 acatagcctc caaaacgtcc tagtttaaag gctaattctg gtgttagttc taggttagct
    44761 tctccacgga ctccatcagt cccaaaatat ttacccattg ttataaaatc cttttctatt
    44821 tttaattcgt ttttgaacta gttgctttcg ttgacgaaga tgtctccgat gaactgcttg
    44881 tacttgaatt tgatgtgctt gaacttggtg ctactggttt tgtagtcacc ttcattattg
    44941 tatcaaacgg agtgataact gccggtaaga caacaccatt gcggtcgatt gcctgcaaag
    45001 gtactgaacc actgtaatta cctgttatgc gttcgctagt tggcaagaca gcgataatct
    45061 tatcaattct atccaatgtc tcttggtcac tcgtaataga cgcttcttta tctgacacca
    45121 tgacattttc aatttgtacc cgactatcaa tttgactagg gtcaatctct ggtacaatct
    45181 ttaccttatc cttctgagcc ttcttaccaa tcttgactgt aattttttgc ggagtcgcca
    45241 cagcggtcag cccattgggt aaatcttcaa tgctcaaagg aacttcaatt gttccaacac
    45301 cggcatctgt taggtcagca gtaaccttga atttacgtgt actttcttgc atttcactag
    45361 ctagcgatag gcgatttgca ccagtcaaga ccactgatac ttctgaagca aaaccgctaa
    45421 taaaatactt atcactatta tagcgtatgt caatagggac atttgttact gtattagtat
    45481 aggtttccgt ttttacctgc ctagcactgg tactgttttg aaaattcgtc gccgtagcat
    45541 agacaaataa gacacaagca aaaaagagtg aggatatgat atataaacta ttttttttca
    45601 tgtttccatc ctcctagcaa tcgttcttta aaactaagac ccacttcctc ttttggaagt
    45661 aagatttcac gtaattctgt ttcaaattca tcaagtgtta ggttgtgctt aaaccttcca
    45721 ttataggtta tcgaaattcc tcccgtttcc tctgatacga caaaagtcaa agcatctgag
    45781 acttctgata aaccgatagc cgcccggtgt ctggtcccaa attccttgga aatccctgtg
    45841 ttttttgtca agggcagata ggcagacgtc acagcgatac gttcttcttt gatgatcacc
    45901 gcaccatcat gtaggggagt gttgggaata aaaatgttaa tgagaagttc tgcagaaatc
    45961 ttagcatcca agggaattcc tgtcgaaata tactcctgca aggtacgtac acgctgaata
    46021 gcaaccaagg ccccgatttt acgaggactc atgtattcaa cagacttaac aaaggcacga
    46081 atcatctgtt cctcagcact aataggggca ttggaaaaaa aatctgtagc tcttcccaaa
    46141 cgttccaaac cagtccgaat ctctggagag aagataacaa ccgccgcaat aaccccataa
    46201 gtaataattt gattgattaa ccaagaaatc gtagtcaaac caatcatatt tgcaaggatt
    46261 tgagctaaaa taaacaccaa aactccacgt accaaaatca taatcttggt tcctgcaata
    46321 gcttttgtaa aatggtataa aatataagca acaatcaaaa tatcaatcag attgatagct
    46381 atcgtccatg gacttgcaaa caaactggtc caatattgca gattggataa ttgttgaaaa
    46441 ttcatccctg atatcctccc tatcaaaaca ctttcgtcct attataccat tttctggcat
    46501 ttttttccct atcctagtcc attttacatt gaacaaaaat atgataaaat aaactgacta
    46561 gaaaaaacaa aggagaaact atgtctcaac tctatgatat taccattgtg ggtggtggtc
    46621 ctgtcgggct ttttgcagcc ttttatgccc acctacgcca agccaaggtt caaatcatcg
    46681 actctcttcc ccagctaggt ggacaacctg ctattctcta ccctgaaaag gaaatcctag
    46741 acgtaccagg cttcccaaac ctgactggag aagagttgac taaccgcttg attgaacagc
    46801 taaatggatt tgatacccct attcatctca atgaaacggt tcttgagatt gacaaacaag
    46861 aagaagaatt tgccatcaca acttctaaag gaagtcacct gactaaaaca gtcatcatcg
    46921 ctatgggtgg cggtgccttc aaaccacgtc cgctggaact tgaaggtgtt gagggctatg
    46981 aaaatatcca ctaccacgtt tctaacattc agcaatacgc tggtaagaaa gtgacgattc
    47041 ttggtggggg agactcggct gtggattggg ctttggcttt tgaaaaaatc gcaccaacta
    47101 cccttgttca ccgcagagat aatttccgtg ccttggaaca cagtgttcaa gccttgcaag
    47161 aatcatctgt aaccatcaag acaccattcg cccctagcca actccttgga aatgaaaaaa
    47221 cacttgataa acttgaaatc acaaaagtca aatctgatga aactgaaacc attgacctag
    47281 accacctctt tgtcaactat ggtttcaaat cttctgtcgg taaccttaaa aactgggggc
    47341 tcgacctcaa ccgtcacaag attatcgtca acagcaaaca ggaatccagc caagcaggta
    47401 tctatgctat cggtgactgc tgctactatg acggaaaaat tgatctgatt gcgacaggcc
    47461 tcggagaagc tccaactgct gtcaacaacg ctatcaacta cattgaccct gaacaaaaag
    47521 tacaaccaaa acactctact agtttataaa aaaaaaccac gagtcacata ggattcgtgg
    47581 ttttataatt catccgctat cttattgatt tttctgagtc tgtgattgac accacttttg
    47641 gtcagagggg tgctgagact atctgctaac tgctggatag agtagtctgg gtgctgaatc
    47701 ctcagttgcg ccacttcctg caaatctact ggcaaatttt ctaagcccat gatatctttg
    47761 attttactga tattgttaat ggtcttcatg ctggcagaaa ctgtccgagc gatattagct
    47821 gtctcggcat tattagcccg attgaggtcg ttacgggttt ctcgcaaaat cttaacccgc
    47881 tcaaaatcat cacgtgcctg catggctccg attactatca agaagtccat aatgtcttct
    47941 gctcgctgga gataggtcac agcccccttc ttgcgctcaa gcaccttggc atccagtaaa
    48001 aactgttgga gaagggaggc aattccttgc gcgtggtcca gataaacaga actgatttcc
    48061 aactggtact tgcctgactc agggtcacga atgctcccat ttgccaagaa agcgccacag
    48121 agataggcac gacctgcttc ctcatccgat aaaatcgcct catcaatacc tgtttccagg
    48181 ccaaagaaag agtctgccaa gtgcaaatca cttaacaaat cctgcacctt ttcatctgta
    48241 aaaacggtat agacgcgatt cttgcgaaga ttgctccgtt ggtggtgacg aatttcagat
    48301 ttgatttcat agaaatggag aaaggactca tagaggtgac gggccagttt ggcattttct
    48361 gtcacaactg acaaagtcaa gcccgaagtc gagagaccga tgctaccaga cattttgata
    48421 atggcagata attcatgcca gctcagatgg tgttggccca ggatttcttc ttttactgct
    48481 actgtgaaac tcattttttc acctgtataa tgcgcatcaa ctcgtccaca atcaaatctc
    48541 catcgtggaa ggcaccgcca ttttccagac gaaggaagtt agatgaaatc acgcgcgaaa
    48601 cttgcttaca aagacctaca aaatcgtgtt ccacttgcac taagtattca tcaaaacggt
    48661 tggaattcat gtattcctga ggcacttttt caatattcac caagacagtg tcgataaaag
    48721 ggcgaccaag gtgacgatgc aagacttcca cgtggtcgct atctgtaaag tgttccgtct
    48781 ccccacgttg ggtcatgata ttgcagacat aggcaatttc tgccttggtt tccaaaagag
    48841 cccgcccaat ttccttaatc acgatattgg gcaaaataga ggtaaagagg gaacctggcc
    48901 ctaggacaat catgtcactt tcaaggatgg tctgcactac tcgacggctg gccagaggcg
    48961 tatcatcgtt tagggcattg gtcacataga cattgtcaat tatgcctcga tggtctacaa
    49021 tatgactctc tccagccact tctgtcccat cctgaaagac tgcatgaagg gtcaaaggat
    49081 ggtcactgga aggataaatt ttccctgttg tatggaaaaa tttgctcaat aactgcatgg
    49141 cattataggt tgaaccctgc atttctgaca agccagcaat gatgagattt cccaatggat
    49201 ggccagcaaa ggctccggca tcctcagaga accgatactg aaagaccttc tcataaaact
    49261 taggcatatc cgacatggcc acaaggacat tacgaagatc acctggcggt gtcaactgtt
    49321 gcatattttt tcggagttca cctgaagaac caccatcatc tgccaccgtc acgatagctg
    49381 cgatttccac atctttttcc cgcagacttt ttagaatgac gggaattcca gtccctccac
    49441 caatcaccgt tatctttggt tttctcatga acggtttacc gtttcctttc tgcggtcttt
    49501 gtcgcgatgc ccttcattaa cagaccaatt cttggataag tcctgcgcca agcgtttagc
    49561 aaatgccaca ctacggtgtt gtccacccgt acatcccatg gcaatggtca aaacggactt
    49621 accttccttt tggtaacttg gcagaatcgg ctcaatcaag gccaataaat gttgataaaa
    49681 gtcttctgac tcaggatggt tcatgacata atcataaaca ggttcatcca cacccgtttg
    49741 gtttctcagt tctggtagat aatagggatt tggcaagaaa cggacatcaa agaccaagtc
    49801 cgcatcaatc gggattccat acttaaatcc gaaagacatg acttcgatac ggaaagactg
    49861 ggcttgttct tggtctgaaa actgctctgc aagggttttg cgcagctcac gtggagtgag
    49921 ttcagtcgta tccaccacat tttggctcat atttttcaaa ggtgccaaga gttcacgttc
    49981 caacttgatt ccatctaaaa tacgaccgtc tgctgctagt gggtgactcc gtctggtctc
    50041 cttgtaacga gcgaccaatt ccttatcagc cgcatccaaa aagaggattt tgaaatccaa
    50101 accatcttga ttttccaact catccaaaac agcttgaatc tctgaaaaga aagaacggct
    50161 acgcatatcc actaccaagg ccaacttagg attgtcttcc ttaatttcaa ccagctgcaa
    50221 aaacttaggc aagagagctg gcggcatatt atcaatggtg aaataaccta gatcctcgaa
    50281 ggactgaatg gctacagttt tccctgcgcc actcatccct gtcacaatca ccaagtgaag
    50341 ttgtttcttt gtcatctttt tctccttata tcaaaagaag tttggcaaca ccaaacttca
    50401 actagcttat ccaatctctg cgatgacttc aatttcgact tttacatcac gaggaagacg
    50461 agctacctcc acagctgaac gagctgggaa ttcctctttg aaggccgttt ggtaaacctc
    50521 attaaaagga acaaagtcgt tcatatcgct caagaagcaa gttgttttga caacatggtc
    50581 aaagtctgtt cctgcttctg ccaaaatagc accgatgttt ttcaagactt gctctgtctg
    50641 ttcttggata ttctctccta caatttcccc agtttcaggg gataggggaa cttgaccgct
    50701 agcaaacaaa aggttgccaa cgatttttcc ttgaacatag ggtccgatag cctttggggc
    50761 cttatctgta tgaattgttt ttgccatttt cttttcctca caatttttct aagattgcat
    50821 cccaagcctc atccatccct gccttgctga cagatgaaaa gaggatgaaa tcgtcacttg
    50881 ggtcaaagtt taatttcttt ttgattgctg attcatgctt gttccattta ccacgaggaa
    50941 tcttgtccgc cttggtcgcc acaatgatga ctggaatctc ataatacttg agaaattcgt
    51001 acatctgcac atcatctgct gacgggtcat gacgaaggtc aactagactg acaaccgcac
    51061 ggagattttc ccgagtcgtt aagtactcct caatcatgca cccccacttt tcacgttcct
    51121 ttttagaaac acgagcatag ccataaccag gcacatccac aaagcgcatc ttgtcatcaa
    51181 tgttaaaaaa gttcaggagc tgggttttac caggttttcc tgatgtacgg gcgagattct
    51241 tacggttcaa catagtgttg ataaagctgg atttaccaac atttgaacgc cctgctaggg
    51301 caatctctgg cagttcatcc tgcggatagt gggacttatt agctgcactg agcaagattt
    51361 cagcattgtg tgtattaagt tccatagtca cctctaggct gtttctagga tcggtttatc
    51421 cgttccatct acagtttctt tagtgatgcg aaccaatttc acattttcct gactcggcac
    51481 ctcaaacatg acatctagca tggtttcttc gatgatggag cgaagtccac gcgcccctgt
    51541 cttccgttcg attgctttat tagcaatctc ttgaagggct tcgtcgtcaa attccaactc
    51601 aacatcatca taagaaagca aggtttggta ttgtttcacc aaggcatttc ttggctcttt
    51661 caagatgcga accaagtcat caacggtcaa ttgctcaaga gccgcaaaaa caggcaagcg
    51721 tccaatcaac tcagggataa taccaaattt ttgaatgtct tcagcgatga tttcttgcat
    51781 gtatgagctg ttttcgtcaa tcgccttatt gttttgacca aatccgataa ctttttcacc
    51841 cagacgttgt ttgacaattt cttcaatacc atcaaaagca ccacccacga tgaagaggat
    51901 attttttgta tccacttgaa tcatctcttg ttgtggatgt ttgcgtccac cttgaggcgg
    51961 cacgctagca acagttccct caataatctt gagaagggct tgttgcaccc cttcaccaga
    52021 aacatcacgt gtgatagaca cattctcact cttcttggca atcttgtcaa tttcatccac
    52081 atagataatg ccacgctctg cacgttcgat gttaaagtca gcaacctgca agagtttgag
    52141 gaggatattt tccacgtcct cacccacata accagcctcc gtcagagctg tcgcatccgc
    52201 aatagcaaaa ggtacattca agctcttagc caaggtctgg gcaaggaaag ttttccctga
    52261 accagttggg ccaatcatca aaatgtttga cttctgcaaa tctacatctt ctgactcttc
    52321 gcgtgtatcg tggaaattga tgcgtttgta gtggttataa accgccactg ccaaggcacg
    52381 cttggcacga tcttgaccaa ttacatagtg gttcaagata tggaggagtt caattggttt
    52441 tggcacctca gacaagtctg ccaagacttc ctcaaccaat tcttctcgaa tgatttcctg
    52501 agctaactcc acgcattcat tacaaataaa agcattgttg ccagcaatta ttttttgtac
    52561 ttcttcttgg tttttgccac aaaatgagca ataaaccatc atatcatttt ttctatttgt
    52621 agacatgatt tccttccatt ctatactgtc attctatcta aaataaggtc atgtaaaaag
    52681 catgaatact attgaccaga ttggtaaagg catttaacca aaggaggata gaaagcccgt
    52741 aacgcttttt acgaaaagcc tgtgctcctg ccagaaagca gatgaaacac agaaaagccg
    52801 tgaatagacc aaataaactc cgttccatta gacttccttt ctcttgcggt attggatggt
    52861 aaaatcataa ggattcttct catctttggc gtaaaatttg cttgaaactg tctcaaaaag
    52921 agacaagtca agctcttcag ggaaataggt atctccttcc acccgagcat gaatgtgagt
    52981 gacaatcact tcatcaaggt aaggtccaaa agcctgaaaa atttgcttcc caccgataat
    53041 gtagagattc ttttcttgag cctgatacca gtcaagaaca gactggacgt cctgaaaagt
    53101 agcaacccca tctatctttt cttccggatt acgcgtcaaa atcaaggttt cccgttttgg
    53161 aagcaagcga cgtcccatcc catcaaaggt cacacgcccc atcaagatag catgattcag
    53221 agttgtttct ttaaagtgct gcaattctgc tggcaaatgc caaggcagac gattttcctt
    53281 accaatcaca ccctcttcat cctgggccca aatagctacg attttcttag tcatgcttcc
    53341 atccttttca ctgatagtac tattttatca aaaaactcaa aaaaagactg gtttggaata
    53401 gcttacaaaa tagaaaaaat ctgtaagaaa tttcctacag atttatctat gtttccttat
    53461 ttcttacaaa ccaggtgctt gtccaagttc ggctgcaagc atccaaattg ttttatctgt
    53521 ttcagtttta gcgcctgcaa agataccgtt tgtcacatcg tcaccttctt catcagtgac
    53581 atccaaacct ttttggaaaa gttctgacaa gtaacggtag ataacaagaa cacgttccaa
    53641 gctttcttca acattacggt attcaccagc ttcttcttcg atttcactat tttgaaggaa
    53701 ctctgtcaat gtagagaatg ggcttccacc gagtgtaatc aagcgttcgc tgatttcatc
    53761 caattgaccg tcaagagctt ccatgtactc atccattttt ggatgccata caaggaaacc
    53821 acgaccatgc atataccagt gcacttggtg caaagcaacg tgagctacat acaaatcagc
    53881 aacagcttgg ttcaagactt cctttgtttt tgccaatgct actggcgctg cttttgtcaa
    53941 tgatgttacg tcttttactg cttctttttt caattctacc attttcttta cctcattcat
    54001 tattttttgt aaccacttct taatgattac taccttagta tactactaag gaaaaccaat
    54061 agcaagacaa agaacttata tgagaaaagt tgcaatagac tgataattta agaattcttt
    54121 agaagacaaa aaagagggtt ggatgcttat agtcaatgga aatccatcag caaactagga
    54181 aactaaaccc agcttgacca aagcacaagt tttgaggtcg caggtaggac tgaccaagct
    54241 gatgtggttt gaagaaattc cccaatacta taatagattg aaataagatg aaaccaagtc
    54301 gattaggaaa ggtaaatgaa tttctaaaat gttttaccaa tcacaaggtc tttatctata
    54361 ttcaatctac tatataagct tcatttccga gcaagatctt gaatttactc gaggatgaac
    54421 tatcataaaa caaagaaaaa gagggtgcta ctatcacatc cctctttcaa atcatcgctt
    54481 aataccaaac gctgacatct acgcgccctt gaattccttt catgtattca gaagaggtat
    54541 attgccaacc tttttctcct gaataataag ggttttccca ttctaaagca ttcgtatagg
    54601 ccgctaccca gtttacatgt tttaaaatat ctgggtgttt taaacgcgtc tgtaataaac
    54661 tacgatagct atagacatac acattttgat aacccgcctg cttcatcgtg tccatgtact
    54721 tgttgatgat tttaacccaa gtgtctgtat cacttggagc tctcttgctc ttatttacat
    54781 attcccagtt ctcaacatca taatagatag ggtaagacag gttcatattg tatttcttta
    54841 taagttcaat ggtctgttta gcgtcattct cagcatcggt ctcattttca gcataggtat
    54901 agagatagac accataagga attcccagac ggtttaactc cttaatgtta tgcgccaatt
    54961 ccttgtcttc tttaccgcta taacctagac gaacaatgac accatccacc tcgttctcat
    55021 caataacctt tttccaatca ttgatacgac cattgtgctc actaatatca atgattttct
    55081 tagcatgttc ggttcccact tgttcttctc tattattaaa gaaatagcgt ttaccatctc
    55141 tgtgaaccca gccgacattc aaatctttct tttctttgag ctcaccagag gccgcaaaga
    55201 tatagcgagt accatccata atcacttcgt cagtcgccat ggcaccactt ccagtcaaat
    55261 agtagttgcc ttgccactca ttccgagcca tatagcccca cttcttgaaa tagtaccatt
    55321 tgccgcccac tttttgccac tcttggttag cataagttcc atcggatttt agataaaaat
    55381 aagcagaata ggctggatca tagagccatt cattttgcat catagctcct tgaccattca
    55441 agaaataact tccttgccat tggcttttag ccatgtaacc ccacttcttg aagtagtacc
    55501 acttatttcc aatcaattgc cattcttgat gagcataagc cccatctgac ttgagataaa
    55561 accaactctt gtaattactg tcatagatcc actcgttttg ggccatatat ccacctgatt
    55621 tcaggtagta atttccatgc cattcattct gcgagtaacg accatctgaa ttaatataaa
    55681 accaggcctt atagtagttg tcaaaaatcc attcactttg agctttagag ccatcatcct
    55741 ttacataata atccccatcc cagtgggcag cagcattagt ctgtacttct gtgctcgttt
    55801 gcgttttatt agaagactga ttttgctctg aactacttga agctgtcgtt gtatcctgcg
    55861 aagtttttgc tacttcagtt tcatttgcag cgacatgact agttgccaag cctagtaaac
    55921 agatacttgc taatccaatt tttattttca atctttcctc tcctataaaa aatgtaacag
    55981 acatctgaat gctgttccac ctagcttttg ctacttactg attattttac aaagtcaagc
    56041 aaagccaaga agctttcagc ttcaagtgac gcaccaccta caagggcacc gtcaacgtct
    56101 gggcaagcca tgtatgaagc aacattttca ggtttaacag aaccaccgta ttgaacacga
    56161 actttgtctg cgacttcttg accaaagtca gcagctacaa cgtcacgaac aactttacac
    56221 attttttgtg catcgtcttg tgaagctgat ttaccagtac cgatagccca gattggctca
    56281 taagcgataa ctgaggcagc aacttgttca gcagtcaatc cagccaatgc agcagatact
    56341 tgagcaccta cgaattcagc agctttacca gcttcgtaag tttcaagtga ttcaccacaa
    56401 cagatgattg gaagcatacc gttcgcaaag attgcttttg cttttttgtt gatatcttcg
    56461 tcagtttcat ggaagtagtc acggcgttct gagtgaccga taacaacgta atcagtaccg
    56521 atttctttca aaacttgtgg gctagtttca ccagtgaaag cacctgcatt ttcaaagtag
    56581 cagttttgag cagcaacttt aaggttcgag ccttttgcaa cagcaagaac agttgtcaaa
    56641 tcaagagctg gagcagcgat acctgcttca acaagatctg atgaaggaag ttttgatgca
    56701 actgcttcaa cgaatgcttt agcttcttct ggatttttgt tcattttcca gttaccagcg
    56761 ataaatggtt tacgtgacat ttcacatacc tcttttttca atttattttc tatttattct
    56821 atcacaatta tacacagctt gcaaatctta ctcggatttc aagcctactt gcatggatta
    56881 atccttccag agatccatgg catttctgaa atctgcagat acctgtgtca actgaggatt
    56941 gcttgcttct ctttctgctc tcttggcctc aatttgagcc acactcttga ttccttcatg
    57001 gcgccagttt ctcaaaattg cctgaatgta cttccagttt ggttttccat tcaaaacagc
    57061 ttcacgaaga gcctccttaa tcaagtcagc actggttcca tcttccttta gtgtcttggt
    57121 caaatcctca atctcaaaag gcgtcaacaa tcgtcccaac tcctgctgga aggtttccac
    57181 caaatccttg agctggtttt gaggtgttag ctggtctgaa cttgaacgaa ctgctccaaa
    57241 caggtcatcc aaacattcca aggccaaact agcatcaaag aacaattcaa tttcgccatt
    57301 taattcgata gtacgatact ggagcagtcc cctttccgtc agattagaaa tggactggtt
    57361 gacatccgaa atttccttgc caatcctttc agcaatctgg cttggcgaca tttcttctaa
    57421 gcctgtcgta ttttgcaaat agaaaaattg ccaaaccaga aaatcgtcgc tagaaggaaa
    57481 gagttcctta aaatgcaaga gcagggcact cggtaaaacc aaggtacctg atttaaaagc
    57541 gtctaaatat gtcataattc ctcttataaa tacccatatg cagatgcatc atcttctatc
    57601 tttctccaat caaaaggcgt ctcctgatag accgcatggt ttacccaatt actgaaaaag
    57661 agggctgcag atgaagacca acaaagacaa ggtacctgat taacatcatc atctttaaag
    57721 taattttctg gaatatgtgg gtcgaaacct gcatcacgat ctcgaaaata ctcttttgcc
    57781 aaggtatcac ggtcatactc caaatgacca aaactataaa tttctcgtaa atcacgactg
    57841 gccaaaatag aaaccccaac ctgaggtcct tctgataaaa tctcgagatt ggtcttgttt
    57901 aagacctctt ccttagaaat ctccgtgtgc cgtgaatgag gggatacata gctatcatca
    57961 aagcctctaa atagaaggtg accctctttt agggtgtcct gaggataaat acctgatagc
    58021 ttactgtcca tctggtattt ttctacacca tagcgcagat aaagcccagc ctgagccccc
    58081 caacagatat gaagggtcga atagacatga gtcttagacc actcaagcat ctgtctaaat
    58141 tcctcccaat agtccacttc ctcaaatggt aaatgctcaa ctggagcacc cgtgatgatc
    58201 atcccatcaa aatactcatc cttgacttca ggaaaagttt tatagaaggt ctccatgtgc
    58261 tctgaacgag ttgttttaga acggtggctc tccatataga gaaaatcaat atccagttgt
    58321 aggggtgtat tagccaagtg gcgcaacaac tgggtctctg tgaccatttt ctgtggcatg
    58381 agatttaaaa ttaaaatctt caaaggacgg atatcttggt gggcagcacg ttgatcatcc
    58441 atgacaaaga tattctctgt ccgtaaaatc tcaacagctg gcaatttttt atcaattcga
    58501 atcggcataa ccctctccta actgtactct ttcaggaata attgcatatg tttgaagaca
    58561 aatctcaaac acttagtcct tttattatac tgcaagaaga tatagttttc aattatagtt
    58621 tttctctaac tagttatagt ctatttttat atcctagtgt agagaaaaca gccctaggga
    58681 ctgttttcat taataatgca taagaacttt gtagtcgtag tcaccaattt tttcacggcc
    58741 gttcaattca tccaattcaa caaggaaggc acaacctgcc ataacaccac caagtttttc
    58801 aatcatctcg atagttgcct taacagttcc acctgtcgcc aaaaggtcat ctacaataag
    58861 aacacgttga cctggcttaa tggcatccgc gtgcatagtc aaggtatcga caccgtactc
    58921 tttttcatag tcagcagaaa taacttcgcg tggcaattta cctggcttac gaacaggcgc
    58981 aaaaccaatt cccaactcaa aggcaactgg acaacccacg ataaatccac gagcttcagg
    59041 tcccacgatc atgtcgactt tcttgtcagt agcatactga acgatttcac gaacagcgta
    59101 gctataagca tttccatcag ccatcaaagg actaatatca cggaaggtaa tgccttcctt
    59161 tggataattt tcaattgttg caatgtaatc ttttaaattc atctttttct ttctttcaaa
    59221 gttttttact ctctattata gcatattttt taagaaagaa aaaaggaaaa gttaacttca
    59281 ataattatct aacgttttga cgatttataa ctagccatcg caataaagcc caatttctgt
    59341 ttattcttag caaacatttt atacatagtt aaaaactgct ttctattctc ctttttacaa
    59401 gcatttacac aaattttcaa agttcctagc aaaccttcgt cataaatcat acccgataat
    59461 ttcattaatg tcatttcacc agtcaatgct ttcacatcac aataacctga ttctatcatc
    59521 acctgttccc aaccatcttg agttaaagga cctacattta catgaattgc ttgtgataat
    59581 tcctgtctga tagactcttt agcttcctta agaagcacat catgtgtcaa gagaagacct
    59641 ccaggtttta atacccttag atattccatt acacattttt tcttagcttg atcggcttgc
    59701 atagtcagca tagcttcatt tataacaata tcaaaactag catcttgata aggaagtttc
    59761 attgcatttg ctctttcaaa actgattaaa tgagcaacac ctgccgttcc agcaaatttt
    59821 ttagccactt ctaaagcttg agcatccata tcaacagcag ttatcttgca accaaaacgc
    59881 tgtgccaact caattgctgt agttccccta ttacacgcaa cctctagtat tctcttttct
    59941 tttgaaaatc ctccttctgc aattaaccaa tctgtggcac gctttccacc tggacgtaag
    60001 cgttttttcc ccaattttgc taaaaactta tgacctgctt ctgacatttg aacacctctg
    60061 ttttttcttc attataacac aaaatgatat tatctgacaa ttctaatcta ttatttgata
    60121 aactagaatc aaaagcatac aagtcaccat aactatagca taaatttatt attacctctc
    60181 tagttcgaat tcaattgctc acgtagttta cgcatatagc ttcacttgta gatggtaccc
    60241 acaaataata ctcaatggaa atcaaagagc aaactaggaa actagccgta agctgctcaa
    60301 agtacatctt tgaggttgca gataaaactg acgaaatcag taatcatacg tacggcaagg
    60361 cgacgttgac gtggtttgaa gagattttcg aagagtatta aagagcatca caatatagat
    60421 gcataatatg acttctctag caaaaaggag agaagcattt tgtttctctc cctagattaa
    60481 ctgatatctc tccactacaa ttgataaaga gatatctttt cttgtagtgc tttgaatttt
    60541 atttatttta gtagattgtt ttttcagttt ctacatcgaa gaagtgtgct ttgttcaagt
    60601 caaatccaag ctcaactgtt gcacctgttt gcaagtagtc acgagcatca acttttgcaa
    60661 caaactcgtc tttaccaact tggcagtaaa ggtgagattc tgaaccaagc agttctgata
    60721 cagagatagt cgcttttaca acacagtctg ggaatgtttc aaggaaagca ggttctgcat
    60781 tcacgtcttc tggacggata ccaaagatca attcttttcc ttcgtagcct ttttcacgaa
    60841 gaactttcaa tgctccttct ggcactttca aacggaaacc gtcagaaaca atttcgctac
    60901 caaccaattt cacggtgatg aagttcatag ctgggcttcc gatgaatcct gcaacgaatt
    60961 tgttaactgg atttttgtaa acttcttgag gagtaccgat ttgttctaca cgtccgatag
    61021 tacctgtacc agcagggttc ttagtagctg acataataac gatacggtct gcaagtgtca
    61081 tcgcttctgt ttggtcgtga gttacataga tagttgtagc tccgatacga cggtgaattt
    61141 tagcgatttc agcacgcatt gatacacgaa gtttggcatc caagtttgac aaaggttcgt
    61201 ccatcaagaa tactttcgca tcacggacaa tcgcacgccc catggcaaca cgttgacgtt
    61261 gaccacctga aaggtcagct ggtttacgtt ccaagaattc tttcaatcca agtatttcag
    61321 ctgcttcttg aacacgtttg ttaatgtctt ctttgctgta tttacgcaat ttcaaaccga
    61381 aagccatgtt gtcataaaca gtcatgtgtg ggtaaagagc gtagttttgg aataccatgg
    61441 cgatatcacg gtcttttgga gctacgtcgt tgacaactac gccatcgata gatgcagtac
    61501 cttctgtaat gtcttcaaga ccagcaatca tacggagtgt agttgattta ccacatcctg
    61561 aaggtcctac gaaaacgata aattctttat ctttgatgtt caagttgaaa tcttcaactg
    61621 aatagtgttc gctgtttgga tattttttgt aaatattttt aagattcaat tctaccatga
    61681 ggtgaactcc ttttgtcttt tgataattat attatatatg aaaacgcttt atatttctat
    61741 ggcaagatga ccaaaaaaat aaacaagttc tgtgcaactt gcacaaaact ttagagaata
    61801 tcggagaatt tgaagaagat aatctttatt attcttctat agacaaaagc agaaacaagg
    61861 atcagttttc ccatattttc
//
LOCUS       DAVKHZ010000004        54402 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000004 DAVKHZ010000000
VERSION     DAVKHZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.004"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            163..564
                     /locus_tag="VWW99_000185"
     CDS             163..564
                     /locus_tag="VWW99_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9538089.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            564..842
                     /locus_tag="VWW99_000186"
     CDS             564..842
                     /locus_tag="VWW99_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9538090.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            832..1605
                     /locus_tag="VWW99_000187"
     CDS             832..1605
                     /locus_tag="VWW99_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9538091.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            1606..2910
                     /locus_tag="VWW99_000188"
     CDS             1606..2910
                     /locus_tag="VWW99_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9538092.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            3484..4362
                     /gene="pstS"
                     /locus_tag="VWW99_000189"
     CDS             3484..4362
                     /gene="pstS"
                     /locus_tag="VWW99_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9538093.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            4362..5279
                     /gene="pstC"
                     /locus_tag="VWW99_000190"
     CDS             4362..5279
                     /gene="pstC"
                     /locus_tag="VWW99_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9538094.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            5269..6153
                     /gene="pstA"
                     /locus_tag="VWW99_000191"
     CDS             5269..6153
                     /gene="pstA"
                     /locus_tag="VWW99_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9538095.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVIHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            6164..6967
                     /gene="pstB"
                     /locus_tag="VWW99_000192"
     CDS             6164..6967
                     /gene="pstB"
                     /locus_tag="VWW99_000192"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9538096.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            6980..7738
                     /gene="pstB"
                     /locus_tag="VWW99_000193"
     CDS             6980..7738
                     /gene="pstB"
                     /locus_tag="VWW99_000193"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9538097.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            7750..8403
                     /gene="phoU"
                     /locus_tag="VWW99_000194"
     CDS             7750..8403
                     /gene="phoU"
                     /locus_tag="VWW99_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9538098.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            8560..9375
                     /locus_tag="VWW99_000195"
     CDS             8560..9375
                     /locus_tag="VWW99_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9538099.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(9395..10666)
                     /locus_tag="VWW99_000196"
     CDS             complement(9395..10666)
                     /locus_tag="VWW99_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9538100.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            10808..11539
                     /gene="budA"
                     /locus_tag="VWW99_000197"
     CDS             10808..11539
                     /gene="budA"
                     /locus_tag="VWW99_000197"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9538101.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            11549..12301
                     /locus_tag="VWW99_000198"
     CDS             11549..12301
                     /locus_tag="VWW99_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9538102.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            12413..13318
                     /gene="murB"
                     /locus_tag="VWW99_000199"
     CDS             12413..13318
                     /gene="murB"
                     /locus_tag="VWW99_000199"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9538103.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            13478..14635
                     /locus_tag="VWW99_000200"
     CDS             13478..14635
                     /locus_tag="VWW99_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538104.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            14616..15422
                     /locus_tag="VWW99_000201"
     CDS             14616..15422
                     /locus_tag="VWW99_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538105.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            15419..16192
                     /locus_tag="VWW99_000202"
     CDS             15419..16192
                     /locus_tag="VWW99_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538106.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            16189..17259
                     /locus_tag="VWW99_000203"
     CDS             16189..17259
                     /locus_tag="VWW99_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9538107.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEVPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            17520..17759
                     /locus_tag="VWW99_000204"
     CDS             17520..17759
                     /locus_tag="VWW99_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538108.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            18034..18519
                     /locus_tag="VWW99_000205"
     CDS             18034..18519
                     /locus_tag="VWW99_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9538109.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            18541..21159
                     /gene="alaS"
                     /locus_tag="VWW99_000206"
     CDS             18541..21159
                     /gene="alaS"
                     /locus_tag="VWW99_000206"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9538110.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYQELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGRRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(21249..22703)
                     /locus_tag="VWW99_000207"
     CDS             complement(21249..22703)
                     /locus_tag="VWW99_000207"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9538111.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            22882..23631
                     /locus_tag="VWW99_000208"
     CDS             22882..23631
                     /locus_tag="VWW99_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538112.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            23612..25246
                     /locus_tag="VWW99_000209"
     CDS             23612..25246
                     /locus_tag="VWW99_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538113.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILDLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            25929..27092
                     /locus_tag="VWW99_000210"
     CDS             25929..27092
                     /locus_tag="VWW99_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9538114.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTAYRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLIN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHHFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            27089..27766
                     /locus_tag="VWW99_000211"
     CDS             27089..27766
                     /locus_tag="VWW99_000211"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9538115.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEASAPGQISLASMKKI
                     REILDEA"
     gene            27756..28610
                     /locus_tag="VWW99_000212"
     CDS             27756..28610
                     /locus_tag="VWW99_000212"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9538116.1"
                     /translation="MKLDGYTRLTAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVVDIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            28629..29696
                     /locus_tag="VWW99_000213"
     CDS             28629..29696
                     /locus_tag="VWW99_000213"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9538117.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            29706..30872
                     /gene="aroC"
                     /locus_tag="VWW99_000214"
     CDS             29706..30872
                     /gene="aroC"
                     /locus_tag="VWW99_000214"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9538118.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNFEELKEAVAKHRDYTKNY"
     gene            30882..31985
                     /locus_tag="VWW99_000215"
     CDS             30882..31985
                     /locus_tag="VWW99_000215"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9538119.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEYLITENTDYTVVIK"
     gene            31996..32334
                     /locus_tag="VWW99_000216"
     CDS             31996..32334
                     /locus_tag="VWW99_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9538120.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            32426..33709
                     /gene="aroA"
                     /locus_tag="VWW99_000217"
     CDS             32426..33709
                     /gene="aroA"
                     /locus_tag="VWW99_000217"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9538121.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            33702..34178
                     /locus_tag="VWW99_000218"
     CDS             33702..34178
                     /locus_tag="VWW99_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9538122.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIENRLGMSIANFFA
                     EKGETAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLRADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            34175..35023
                     /gene="pheA"
                     /locus_tag="VWW99_000219"
     CDS             34175..35023
                     /gene="pheA"
                     /locus_tag="VWW99_000219"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9538123.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            35020..36294
                     /gene="psr"
                     /locus_tag="VWW99_000220"
     CDS             35020..36294
                     /gene="psr"
                     /locus_tag="VWW99_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9538124.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            36573..37418
                     /locus_tag="VWW99_000221"
     CDS             36573..37418
                     /locus_tag="VWW99_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9538125.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EYNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            37408..38511
                     /locus_tag="VWW99_000222"
     CDS             37408..38511
                     /locus_tag="VWW99_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9538126.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEEFLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            38513..39499
                     /locus_tag="VWW99_000223"
     CDS             38513..39499
                     /locus_tag="VWW99_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9538127.1"
                     /translation="METALISVIVPVYNVAQYLEKSIDSIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAYFFILDKMLLDDQYKQFKAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            39515..40948
                     /locus_tag="VWW99_000224"
     CDS             39515..40948
                     /locus_tag="VWW99_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9538128.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            40977..42011
                     /locus_tag="VWW99_000225"
     CDS             40977..42011
                     /locus_tag="VWW99_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9538129.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            42211..42948
                     /gene="mecA"
                     /locus_tag="VWW99_000226"
     CDS             42211..42948
                     /gene="mecA"
                     /locus_tag="VWW99_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9538130.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            43099..44385
                     /locus_tag="VWW99_000227"
     CDS             43099..44385
                     /locus_tag="VWW99_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9538131.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            44387..45256
                     /locus_tag="VWW99_000228"
     CDS             44387..45256
                     /locus_tag="VWW99_000228"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9538132.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            45332..45409
                     /locus_tag="VWW99_000229"
                     /pseudo
     CDS             45332..45409
                     /locus_tag="VWW99_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            45551..46489
                     /gene="msrB"
                     /locus_tag="VWW99_000230"
     CDS             45551..46489
                     /gene="msrB"
                     /locus_tag="VWW99_000230"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9538133.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            46547..48271
                     /locus_tag="VWW99_000231"
     CDS             46547..48271
                     /locus_tag="VWW99_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538134.1"
                     /translation="MKYLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLIQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            48273..50021
                     /locus_tag="VWW99_000232"
     CDS             48273..50021
                     /locus_tag="VWW99_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538135.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            50413..51672
                     /locus_tag="VWW99_000233"
     CDS             50413..51672
                     /locus_tag="VWW99_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9538136.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    51748..51883
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            51922..52422
                     /locus_tag="VWW99_000234"
     CDS             51922..52422
                     /locus_tag="VWW99_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9538137.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNNFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            52498..52866
                     /gene="rplL"
                     /locus_tag="VWW99_000235"
     CDS             52498..52866
                     /gene="rplL"
                     /locus_tag="VWW99_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9538138.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            53067..53240
                     /locus_tag="VWW99_000236"
     CDS             53067..53240
                     /locus_tag="VWW99_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538139.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQS"
     gene            53237..53485
                     /locus_tag="VWW99_000237"
                     /pseudo
     CDS             53237..53485
                     /locus_tag="VWW99_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            53499..53822
                     /locus_tag="VWW99_000238"
     CDS             53499..53822
                     /locus_tag="VWW99_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538140.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLW
                     EVSHD"
     gene            53815..54195
                     /locus_tag="VWW99_000239"
     CDS             53815..54195
                     /locus_tag="VWW99_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9538141.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            54208..54351
                     /locus_tag="VWW99_000240"
     CDS             54208..54351
                     /locus_tag="VWW99_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538142.1"
                     /translation="MAERFWENLSIILAERNISCIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
BASE COUNT        15713 a        10260 c        12297 g        16132 t
ORIGIN      
        1 aaaccatcgt taagagtgaa ttaaccaaat ccaattgtgt caatcgttcg caaacagcct
       61 atttttcttg aaaaaatagg ctttttttct aaaaatccct agtcaagcgc cttatttttt
      121 tgtataatag aagtagcaaa gtatagataa gaagagaaaa gcatgattac actatttcta
      181 tcaccgagct gtacatcatg tcgtaaggct aaggcttggt tagaaaaaca taaggttccc
      241 tttgtggaac acaatattat gaccagtcct ttaacaagaa aagaattgca acacatcctt
      301 tccttgaccg aaaatggtac tgatgacatc atttcaactc gttcaaaaat ttttcaaaaa
      361 ttgaatattg atgtagagag tatttcggta tcggaattgc ttcatttgat tgagcagtat
      421 cctagtcttt tgcgtcgtcc aattattatt gatgccaagc gtatgcaaat cggttttaat
      481 gaagatgaga ttcgtgcttt tctccctcgt agttatcgta agcaagaact aaaagaagca
      541 agaatgagag ctggtattag ttaatgaaca aacagtatag ttacccacta gatttgtcgt
      601 ggagcactga agaacttgct tcagtgcttt ctttttttaa tgatgttgaa gctgcctatg
      661 aaggcaaagt agaggctaaa aagttactgg actcctataa gggattcaaa gcggtcgtgc
      721 caagcaagag cgaagagaaa cgtttgggac gagaatttga aacggttagt ggctattcac
      781 tttaccgagc agttcaggct gccaaagaaa aaggggaagg aaagatttct cttggaaagt
      841 aaatttgaat ttgctaaaga gcttgttaag aaagcaggtc agtacattct tgaccatatg
      901 caggaagact tgcgtgttga aaccaagtcc tctccaacag atttggtgac cagactggac
      961 aaagaagttc aggaactctt ggttggtgag attttgtccc gttatcctga ggataagatt
     1021 tgtgctgaag aaggttgtct gcgagcctcg gttcaagagg gcaaggtttg ggtcattgat
     1081 cccattgatg gtaccaataa ttttgtagcc cagcaggaag attttgctgt tatgatggct
     1141 tattttgaaa atggtcaggg acagtttggt ctgatttatg atgtggtcaa aggggattgt
     1201 taccacggtg gtggggaatt tccagtttgt ctaaatgata ggcccctagc tccttttaaa
     1261 actaaaccac ttggagattt tctcattgca gggaatagtg gtatgctgga aaccaatgaa
     1321 tgggggctgg ctgatttgag ccgatcggtt cttggtgttc gtgtctatgg aagtgcggcc
     1381 attagttttg ccaagatttt gtcagggcgt ttgttgacct atctcactta tctgcaacca
     1441 tgggattatg ctgcagctag tattttaggg gaaagtctgg gctatcgggt tgtgaccctt
     1501 tttggtgaag ctcctgattt tcaaaccaga cagccggtca tgatggtgcc tcttgagatg
     1561 caggaggaaa ttcagtccta tatttacgaa aggaaaagaa cttaaatgca atttccagaa
     1621 ggatttgttg aaaaatataa agagatacta ggagatgagg caagagattt tcttgcctct
     1681 tttgaggagg aagcggtttc ggcctttcgg gtcaatccct taaaagaaga gcaactttcc
     1741 ttttctgatg ccattactca aaccccttgg ggccactatg ggaaggtttc agggaaatcg
     1801 cctgagcatg ctacaggttt agtttattcg caagaacctg ctgctcaaat ggtggctcaa
     1861 gtagcccaac ccagtcctgg tatgaaggtc ttggacttgg ctgctgcacc ggggggcaaa
     1921 tcaactcaac tggcagccta tctagcaggg gaggggctac ttgtttccaa tgaaatttca
     1981 agcaaacggg ctaagatttt ggttgaaaac atggagcgtt ttggagcgac aaatgtcgtg
     2041 gtgaccaatg aatctgccga ccgcttggtc aaggtcttta aaggctattt tgacttaatc
     2101 gttcttgatg ccccttgctc tggtgaagga atgtttcgta agcaacctga tgctatggac
     2161 tattggagct tagattatcc gagtcaatgt gctagcttgc aaagagaaat tctggaggat
     2221 gcagtgacca tgttagctga aggtggtcgt ctggtttatt cgacctgtac ctgggcaccc
     2281 gaggaaaacg aagagattgt caattggttg ctggaagagt atgattttga cttgttgcca
     2341 gtagagcata tcaatggaat ggtagctggt attgacctgc cagaaacggc tcggatgtac
     2401 cctcatcagt ttaagggaga gggtcagttt gttgcccatc tacagtttaa gggtaacaat
     2461 ccagctccta aatttaaggc aagtaagagt aacctcagcc gtgaacaagt tgccttgtgg
     2521 caggaatttg cccaaaacca tttgaaggtc aatctaccgg gtatcttgca gatttttgga
     2581 gaccagcttt atctcttgcc agaacttttg ccagatttag ggaagctcaa gattgctcgc
     2641 aatggactgc atctgggtac ttttaagaag aaacgctttg agcccagttt cgctcttggt
     2701 ttagccttga aaccgagtca ggtcgaacag tcggttgaaa ttggccaaga agcgtttgtc
     2761 aagtatgcgg ctggagaaac cgttcagctg gcagaaagtc tgccaaatgg ttggtaccaa
     2821 gttttggtta aaggcaatgg cttgggcttt gcaaaggtta ctggaaatgt tttgaaaaat
     2881 tattttccaa aaggcctcag attcaaatga aaatgctagt caaatatctt attctatgtt
     2941 atagtgaaag tatggatttt tttagtagga tgattcttga tatcttgatt gccattacat
     3001 gtagttttac tactcaatga aaatcaaaga gcaaactagg aagctagcca tagatagctc
     3061 aaaacactgg tttgaagaga ttttcaaaga gtacaagaag tttagttatt agcgttctta
     3121 ccgcttgtaa actagatttc tcataaaata gaatcttttc cttttagttg taaactagtc
     3181 tgggagagta gagaggtttg agataccttt ctagcttttg gattatcatc taaggagagt
     3241 aatttccctt gcattaaaaa ggagaaaaag agacacgaaa tgactataat gggtgacaat
     3301 gggggaaggg atagacaaga gattttatcc acatatgaaa aaaggaggtt aggaaagagt
     3361 aatatagcct atattatata aataatcaat tgcgcagaaa tttggtaaga attcatgcgt
     3421 caactcataa agaactactt aaaaaattca cagtattcat aattattttc gaggagaaaa
     3481 acagtgaaaa aaagaaaaaa gcttgctctg tctcttatcg ctttttggct gacggcttgt
     3541 ttagtaggct gtgctagctg gattgatcgt ggagaatcca taacggctgt tggctcaact
     3601 gccttgcaac ccttggttga agtagcggca gatgaatttg gcacgatcca tgttggaaaa
     3661 acggtcaatg tccaaggggg aggttctggt acaggcttgt cccaggttca gtctggggca
     3721 gttgatatag gaaactcaga tgtatttgct gaggaaaaag acggaattga tgcttctgct
     3781 cttgttgacc acaaggtcgc ggtagctggc ttggctctga ttgtcaataa ggaggttgat
     3841 gttgataacc taacgacaga gcaacttcgt caaatcttca taggtgaggt aaccaattgg
     3901 aaagaggttg gtggtaagga cttacccatc tctgttatca atcgggcagc cggctctggt
     3961 tctcgtgcta cctttgatac tgtcattatg gaaggtcagt ctgccatgca aagtcaggag
     4021 caggattcaa atggagcggt aaaatcaatc gtatcaaaaa gtccaggagc tatctcttat
     4081 ttatctctta cctatataga tgattcggtc aaaagcatga agttgaatgg ctatgactta
     4141 agtccagaaa atataagtag caataattgg cccttgtggt cttatgagca tatgtataca
     4201 ttggggcagc ccaatgagtt ggctgcagaa tttctcaatt ttgttctctc ggatgagacc
     4261 caagaaggga ttgtcaaagg attgaagtat attccgatta aggaaatgaa ggttgaaaaa
     4321 gatgctgccg gaactgtgac agtgttggaa gggagacaat aatgaatcaa gaagaattag
     4381 ctaagaaaat gttgcttcca tcaaagaatt ctcgtctgga gaaattagga aaaggtttga
     4441 cctttgcctg tctttctttg atagtcatcc ttgtggccat gattttggtt ttcgtagcgc
     4501 aaaaaggctt gtcgaccttc tttgtcaatg gtgtgaatat ctttgacttt cttttgggag
     4561 gaacttggaa tccttctagt aaagaatttg gtgcccttcc tatgattttg ggttccttta
     4621 tcgttaccat tctctcagcc cttatcgcaa caccctttgc tattggtgca gcagttttta
     4681 tgaccgaagt atcaccaaaa ggggcgaaga ttttacaacc agctattgaa ctcctggttg
     4741 ggattccttc agtagtgtac ggatttattg gcttgcaagt cgtcgttccc tttgttcgca
     4801 gtgtctttgg tgggactggt tttgggattt tgtcagggat ttccgtcctc tttgtcatga
     4861 ttttgccgac cgtaaccttt atgacaacgg atagcttgcg tgcggttcct cgttattatc
     4921 gtgaggccag tttcgctatg ggagccactc gctggcagac tatctggcgt gtgatcttga
     4981 aggcggcccg ttctggtatt ttcactgcag tggtctttgg gatggcgcgt gcctttggtg
     5041 aggctttagc tatccagatg gttgtcggaa actcagctgt tatcccaact tccttgacca
     5101 caccagctgc aactttaact tctatattaa ctatgggaat tgggaacact gtcatgggaa
     5161 ctgtaaataa taatgttctc tggtcactgg ccttggtact gctcttgatg agtttagttt
     5221 ttaacagtgt gattaaattg attacgaaag aaagaggaaa gaaaaattat gcgcgctaag
     5281 aaattagata aacttgcaac agctgtcctc tatacgattg ctagcatcat tgtgacaatc
     5341 ttggcttcct tgattctcta tatcttggtt cggggcttgc cccatatctc ttggtctttc
     5401 ttgactggaa ggtcttctgc ttttcaagca ggtggtggga ttggcattca gctttacaat
     5461 tcctttttcc tattggtcat taccttgatt atttctgtac ctctttctat gggagctggg
     5521 atttacttgg ctgaatatgc taaaaaaggt cctgttacca actttgtgcg gacttgtatt
     5581 gaaatcttgt cctctttacc atcagtggtg gtgggtctct ttggttactt gatctttgta
     5641 gtccagtttg agtatggatt ttcaatcatt tcaggtgcct tggccttgac agtctttaac
     5701 ttgcctcaga tgacgcgtaa tgtagaagat agtttgaaac acgttcacca tacccaacgt
     5761 gaggctggtc tggctcttgg gatttctcgc tgggagacag tgattcatgt tgttattcca
     5821 gaagcgcttc caggtattgt aacgggtgtc gtcttggcat ctggtcgtat ctttggcgaa
     5881 gctgcagctc tgatctatac agcagggcaa tcggcgccag ctcttgactg gtctaactgg
     5941 aatatcctca gtgtgactag ccccatctct atcttccgtc aagcagaaac cttggctgtc
     6001 catatctgga aagtcaatag tgaaggcact attccagatg gaaccattgt atcagcaggt
     6061 tctgccgctg tgctcctgat ctttatcctg atttttaact ttggagctcg taagttcgga
     6121 agctatctac acaagaaatt aaccgctgcc taaaggagaa gccatgtcaa catataactg
     6181 ggatgagaag catatcctta cctttcctga agaaaaagta gccctttcta ctaaggatgt
     6241 ccatgtttac tatggtaaaa atgaatccat taaggggatt gatatgcaat ttgaaagaaa
     6301 taaaattaca gctttgattg gtccgtcggg atcggggaaa tctacctact tacgcagtct
     6361 caatcgcatg aatgatacta ttgatattgc taaagtaact gggcagattc tctatcgtgg
     6421 aattgatgtc aaccgtccag aaatcaacgt ttatgaaatg cgtaaacaca ttggaatggt
     6481 ttttcaacgc cccaatccat ttgctaagtc aatttaccgt aatattacct ttgcgcatga
     6541 acgtgctgga gttaaggata agcaagtcct agatgaaatc gtagaaacct cccttcgtca
     6601 ggctgccctc tgggatcagg ttaaagacga tctccacaag tcagccttga ccttatcagg
     6661 tggtcagcaa caacgtctct gtatcgctcg tgccatctct gttaagccag atatcctctt
     6721 aatggatgag ccagcctcag ccttggatcc gattgcgacc atgcaactag aagagaccat
     6781 gtttgagctc aagaaaaact ttaccatcat cattgtaacg cataatatgc agcaggctgc
     6841 tcgtgcaagt gactatacag gcttctttta cttgggtgat ttgattgagt atgacaagac
     6901 tgcaactatt ttccaaaatg ccaagctaca gtccaccaat gactatgtat ctggtcactt
     6961 tggttagaaa ggaaaccgta tgacagatgc gattttacag gtatcagacc tgtccgttta
     7021 ttataataaa aagaaggctt tgaatagtgt ttccctatct ttccaaccta aggaaattac
     7081 agccttgatt ggtccatctg gatcagggaa gtcaaccctc ctcaagtctc tcaaccgcat
     7141 gggagatctc aatccagagg tgaccacaac tggatccgtg gtgtacaatg gtcacaacat
     7201 ctacagtccg cgtacagata cggttgaatt acgtaaggaa atcggaatgg ttttccaaca
     7261 acctaatcct ttccctatga ctatctatga gaatgttgtc tacgggcttc gtatcaatgg
     7321 aattaaggat aagcaggttc tggatgaagc cgtagaaaaa gccttgcaag gtgcctctat
     7381 ctgggatgag gtcaaggatc gtctatatga ttcagctatt ggattgtcag gtggtcaaca
     7441 gcagcgtgtc tgcgtggccc gtgtcttggc aactagtcct aaaatcatcc tcttggatga
     7501 gccaacttcg gctttggatc cgatttcagc tggtaaaatt gaggaaactt tgtatggtct
     7561 aaaagacaag tacaccatgc ttctggtaac ccgttccatg cagcaagctt cacgtatctc
     7621 tgataagaca ggatttttcc tagatggaga tttgattgaa tttaatgata ccaagcagat
     7681 gttccttaat ccccaacaca aggaaacgga agactatatt acaggaaaat ttggataagg
     7741 agatgaaaga tgttacgatc tcaatttgaa gaagatttag agaaattaca taaccagttc
     7801 tacgctatgg gacaagaagt gctctcacaa atcaatcgta cggtacgtgc ttttgtcacg
     7861 catgaccgtg acctggcaaa agaggtcatc gaagatgatg cagaagtaaa tgaatacgaa
     7921 gtgaaactgg aaaagaaatc atttgaaatg atcgcactcc aacaaccagt ctctcaagat
     7981 ttgcgtacag tcttgactgt ccttaaggct gtatcagatg tggagcgtat gggggatcac
     8041 gctgtagcca ttgctcaggc aaccatccgt atgaaggggg aagagcgcat tccagctgta
     8101 gaggaagaaa ttaaaaaaat gggacgtgaa gttaaaagcg ttgttgaagc agcacttgat
     8161 ctttatctta atggttctgt tgacgacgca taccgggtgg cctccatgga tgagcaaatt
     8221 aaccactatt ttgaaactat ccgtgacctt gcgactgaag agattaagaa aaatccagaa
     8281 gccattgtta cgggtcgtga ttatttccaa gttatttcct acttggagcg tatcggtgac
     8341 tatgctaaaa atatctgtga atgggttgtc tactttgaaa caggtaagat tgtcgaacta
     8401 taaaatagtt taattctgta taaaaaggag cttgaaatca actgatggct cctttttttg
     8461 aacgaaaaaa tatttttgag ataaaaattt aaaaaacact tgacaagtaa cttggatatc
     8521 gttataatta tacaaaatta acagagaggt tgtttattta tgaaatcaaa aaaatggata
     8581 tttgttttat gtaattttct tgcaagtttc ttcttagtag cttgccagtc aggttctaat
     8641 ggttctcagt cagctgttga ggccattaag caaaagggga aattagttgt ggcaaccagt
     8701 cctgactatg caccctttga atttcaatca ttggttgatg gaaagaacca ggtagtcggt
     8761 gcagacatcg acatggctca ggctatcgct gatgaacttg gggttaagtt ggaaatctca
     8821 agcatgagtt ttgacaatgt tttgaccagt cttcaaactg gtaaggctga cctagcagtt
     8881 gcaggaatta gtgctactga cgagagaaaa gaagtctttg atttttcaat cccatactat
     8941 gaaaacaaga ttagtttctt ggttcgtaag gctgatgtgg aaaaatacaa ggatttaact
     9001 agcctagaaa gtgctaatat tgcagcccaa aaagggactg ttccagaatc aatggtcaag
     9061 gaacaattgc caaaagctca attaacttcc ctaactaata tgggtgaagc agtcaatgaa
     9121 ttgcaggctg gaaaagtaga tgctgttcat atggatgagc ctgttgcact tagttatgct
     9181 gctaaaaacg ctggcttagc tgtcgcaact gtcagcttga agatgaagga cggcgacgcc
     9241 aatgccgttg ctcttagaaa aaatagtgat gatttgaaag aagtggtaga caaggtcatc
     9301 caaaaactta aggatgaagg aatttaccaa agctatcttg aaaaagcagc aagtctaact
     9361 gaagttgaag aataagaaaa agcagagttg gatgtcattc caattctgct tttaaatagt
     9421 ttaaaacatt ttccagattg tctagggaca ccttggcaaa ttgataagga caggtactta
     9481 aataggcctc ttcaaagaag tccaagcccg gttgactgtg tttgagactg gcgattttag
     9541 gatactgtct caggtctagc aataaaccat cgtgtagggg ataatgagga aggggacaag
     9601 gtgttttagt tatcctttcc tcgatttgtt tttggtaaga ttcatgattg gtcaagcgag
     9661 ccaaacgatt tttttcaaac aattgactgt caatatagag tgacagggcc ttttgcataa
     9721 tgaggttgct gtcgtagtct aggatatttt tgtaggccac aaatgcttct ttgatagcat
     9781 ttggaagaat gagtgctgta atacgaaggg caggaaaaag gctggtcgag aaggacttga
     9841 tataaatgac atgctcctct gtatcaagat agtggaaggt ttggcccttc ttggagtcca
     9901 aatcacccag ataatcgtct tctacgatat agacatcata cttggcagct aagttaagaa
     9961 tggatcgttt gtcttgctca gaataggaat gtcccagagg atagtgaaat cggggaatgg
    10021 tgtagaaaaa cttaattttt cctgttttga agtggccttc cagctcctcc aagtcaatcc
    10081 catcaatgcc tcgttcaatc gtttgatagt ccagcccctg tgcaatcaag aggcgattca
    10141 tccgatggta ggttggctgt tccaccaaga tttccttggc ttgtctagga aaggatattt
    10201 gagagaggat aaataaggct tgttgggttc cagaagtcag tactagttgg ttagccttgc
    10261 agtagagagc ttgctcaaag aggagtttgt gaatggactg tcttaggtct tctaatcctt
    10321 cttgattgtc atagtagttg aagaggtagt tttctcgtcc aatcaaggtt tcattgacac
    10381 agagtcggaa atcgtcatag gcactggcat gttcgtcggt aacctcgatt tctaggtctt
    10441 gatgttgccc ttgttctagg acatagtagc cgctctgggg cttggcatag aggtattgtt
    10501 cgtgccgtaa ttccagcagg gctcgttgaa tggtgtcctt gctgcagtga aagtcaaggc
    10561 tcagttgacg gatagaaggc aggcgactac ccgtcggaaa tcgttcagac tcgataccat
    10621 ttttcagata ggaaacgacc tctttgtact tgctttgttt cttcattcca gacctccctt
    10681 gattttgtta cattgtaccc ttttttttcc ttcttggcaa tgtctagagc gtttctctcg
    10741 ttcacatcta ggggcaaatg tggtatactt aggagtaaga tttatagaat aacagacaag
    10801 aaagtgaatg gataagaacg tgcaggaacc agtgaaatta tttcaataca atactcttgg
    10861 agccttgatg gctggccttt atggtggtac tatgacagta ggagaattgc tagaacatgg
    10921 tgaccttggt ttgggaacct tggattccat tgatggggaa ttgattgtct tggatggcaa
    10981 ggcttatcag gccaaagggt caggagacca gccagaaatt gtggaagtgt caccagatgc
    11041 ccttattcct tacgctgcag tggtaccgca tcaggcagag gtcattttcc gccagcgctt
    11101 tgagatgaca gacaaggaat tgaaagaacg aattgagtct tattatgatg gggaaaatct
    11161 tttccgctct atcaagattc ggggggaatt ttcacatatg catgtgcgta tgattcctaa
    11221 gtcaacaccc gataccaagt ttgctgatgt cgcaacccat caaccggaat atagtcgtga
    11281 caatgttgcg gggactattg ttggtttctg gacgcctgag attttccatg gggtcagtgt
    11341 ggcaggctac catctgcact tcatatcaga tgatttgacc ttcggtggac atgtcatgga
    11401 ttttgtcatc aaggaaggca ttatcgaggt gggagcagtt gaccagttgg accaacgttt
    11461 cccagtccaa gaccgtcaat acttgtttgc taagttcaat gttgacgaga tgaaaaaaga
    11521 tattgaaaag gcagaatagg agaagaaaat gaccattcat atcattatta ccatgttgct
    11581 gttgctagct tttctgatag gaagcatttg gtttgccaaa aagaaatacc agattaatct
    11641 agctgtcttg ggcttgggtg cagtcgcttt ctttgtctct tcgcagattt tggaaaaact
    11701 ggtgcatatc ttgattttgc accctcaaaa agacggtagt attgccctct tgcaagatca
    11761 tccgcttatc tatattatct atggcctagc catggcagca ttttttgagg aaacggctcg
    11821 tcttgttttc ttcaaatggt tggagaaaaa gagaagtttg gaaaaggcag acgccttggc
    11881 ctatggactg gggcatggtg gcttggagtt gatttttcta ggccttacta gtttactcaa
    11941 tctttacatt gttctttcgg cagttcaaac tcagaatcca caggttatgc aattactgtc
    12001 tgaaaatatg ttaaaaacca ttcagtcact ttcggtctgg cagatttatt tgcttggttt
    12061 tgagcgaatc ttggcgcttg gtttccaatt acttttgaca gtttgggttt accaagctgt
    12121 tcgccagaag aaatggattt atctcctagc agcctatggc ttgcatgcct tctttgatct
    12181 ggcaccatct cttttccaag taggctggtt gacaaatcca gtcttggttg aagtgattct
    12241 agcactggag ctcgttctgg ttgcctatgg aaccaaggaa atcttttgta aaaaatcata
    12301 aaaagggggg aacctctttt tattatgcaa aatccaaaca aggtattttt atggtcgtca
    12361 aatgtctcta aaaatggtat aatggaatga attttgtaaa aggaagagtg acatgtctgt
    12421 aagagaaaaa atgcttgaaa tcttagaagg aattgatatc cgttttaagg aacccttgca
    12481 tagctatagt tatacaaaag taggtggaga ggctgattat ttggtctttc cacgaaatcg
    12541 ttttgagttg gcgcgccttg tgaaatttgc caaccaagaa aatatccctt ggatggttct
    12601 tggcaatgca agcaatatca tcgttcgtga tggtgggatt cgtggatttg tcatcttgtg
    12661 tgacaagctc aataacgttt ctgttgatgg ctataccatt gaagcagaag ctggggctaa
    12721 cttgattgaa acaactcgca ttgccctccg tcatagtttg actggctttg agtttgcttg
    12781 tggtattcca ggaagcgttg gcggtgctgt ctttatgaat gcgggtgcct atggtggcga
    12841 gattgctcac atcttgcagt cttgtaaggt cttgaccaag gatggagaaa tcgaaaccct
    12901 gtctgctaaa gacttggctt ttggttaccg ccattcagct attcaggagt ctggtgcagt
    12961 tgtcttgtca gttaaatttg ccctagcccc aggaacccat caggttatca agcaggaaat
    13021 ggaccgcttg acgcacctac gtgaactcaa gcaacctttg gaatacccat cttgtggctc
    13081 ggtctttaag cgtccagtcg ggcattttgc aggtcagtta atttcagaag ctggcttgaa
    13141 aggctatcgt atcggtggcg tagaagtgtc agaaaagcat gcaggattta tgatcaatgt
    13201 cgcagatgga acggccaaag actacgagga cttgatccaa tcggttatcg aaaaagtcaa
    13261 ggaacactca ggtattacgc ttgaaagaga agtccggatc ttgggtgaaa gcaagtagga
    13321 agctgtattt gaaaagctgc tgctatgtca tggaaagggg gtgaaagcct atcggtagcg
    13381 aagatgtatg caggtggttt tactccctgc aagaggtagt ggggacctga cagagccccg
    13441 atcggttaat ctatgaaaaa gaaggaattt atgccaattg aaaaaaccaa ttatcgaatt
    13501 caaaaacgtc tctaaagttt ttgaagacag caacaccaag gttctcaaag acatcaactt
    13561 tgagttggaa gaagggaaat tctacaccct tctaggtgca tctggttcgg ggaaatcaac
    13621 tatcctaaac attattgcag gtttactgga tgcgacgaca ggagatatca tgctagacgg
    13681 tgttcgtatc aatgatattc caaccaacaa gcgcgacgta cataccgtct tccaatccta
    13741 tgccttgttc ccacatatga atgtgtttga aaatgttgcc tttccacttc gcttgcgtaa
    13801 aattgataag aaagaaatcg agcagcgtgt agcggaagtt ctcaagatgg ttcagttgga
    13861 aggttatgaa aaacgttcca tccgcaaact ttctggagga caacgtcagc gtgttgccat
    13921 cgcccgtgct atcatcaacc aaccccgtgt ggtcttgttg gacgagcctt tatcagcgct
    13981 ggacttgaaa ttgagaacag acatgcagta cgaattgcgt gaattacaac aacgattggg
    14041 cattaccttt gtctttgtca ctcacgatca ggaagaagct cttgccatga gtgactggat
    14101 tttcgttatg aatgatggcg agattgtcca gtctggaacc cctgtggaca tctacgatga
    14161 gccaatcaac cactttgttg ccacctttat cggggagtca aacatcttgc caggtaccat
    14221 gattgaggac tacttggtcg aatttaacgg caaacgcttt gaagcggttg atggtgggat
    14281 gaagccaaat gaacctgttg aggtcgttat tcgtccagag gacttgcgca ttacccttcc
    14341 tgaagaaggc aagctccaag ttaaggtcga tacccagctt ttccgtggag ttcattatga
    14401 aattatcgcc tatgacgaac ttggaaatga atggatgatc cactcaaccc gtaaggctat
    14461 cgtgggtgag gaaatcggtc tggactttga accagaagac atccacatca tgcgtctcaa
    14521 tgaaaccgaa gaagagttcg atgctcgtat tgaggagtac gtagaaatcg aagagcaaga
    14581 agcaggtttg atcaatgcaa tcgaggagga aagagatgaa gaaaacaagc tctaaactct
    14641 ttgtagtacc ctacatgctt tggattgcgc tctttgtatt ggcacccttg gtcttgattt
    14701 tcggtcaatc ctttttcaac atcgaaggcc agtttagttt agaaaattat aaatcttact
    14761 ttgcgtcaca aaacttgacc tacctcaaaa tgagtttcaa ctcagtgctt tatgcaggga
    14821 ttgtgacctt tgtgaccttg cttatcagct atccaacagc cctctttttg acccgtctca
    14881 agcaccgtca actctggctc atgctgatta tccttcctac ctggatcaat ttgctcctta
    14941 aagcctatgc ttttatcggg atttttggtc aaaatggctc tattaaccaa ttcttggaat
    15001 ttatcggaat tggttcacaa cagttgcttt ttaccgattt ttcctttatc tttgtcgcaa
    15061 gctacatcga gctccccttt atgattttgc cgattttcaa tgtcttagac gacatggata
    15121 ataatctcat caatgctagt tatgaccttg gtgcaactaa gtgggagacc ttccgtcatg
    15181 tcatcttccc tctatctatg aacggtgtgc gaagtggggt tcagtcggtc tttatcccaa
    15241 gtttgagtct cttcatgctg acccgtttga ttggtgggaa ccgcgttatc accttgggga
    15301 cggctattga gcagaatttt ctaaccaatg acaactatgg tatgggttca accatcggtg
    15361 tgattctcat cctgaccatg ttcatcacca tgtgggtgac taaggaaagg agagaacgat
    15421 gaaaaaattt gccaaccttt atctgggact ggtctttctg gtcctctacc tgcctatctt
    15481 ttacttgatt ggctatgcct ttaatgctgg tgatgatatg aatagcttta caggttttag
    15541 ctggactcac tttgaaacca tgtttggaga tgggagactc atgctgattt tggctcagac
    15601 atttttcttg gccttcctat cagccttgat agcgaccatt atcgggactt ttggtgccat
    15661 ttacatctac cagtctcgta agaaatacca agaagccttt ctatcactca ataatatcct
    15721 catggttgcg cctgacgtta tgattggtgc tagcttcttg attctcttta cccaactcaa
    15781 gttttcactt ggctttttga ccgttctatc tagtcacgtg gccttctcca ttcctatcgt
    15841 ggtcttgatg gtcttgcctc gactcaagga aatgaatggc gacatgattc atgcggccta
    15901 tgacttggga gctagtcaat ttcagatgtt caaggaaatc atgcttcctt acctgactcc
    15961 gtctatcatt actggttatt tcatggcctt cacctattcg ttagatgact ttgccgtgac
    16021 cttctttgta acaggaaatg gcttttcaac cctatcagtc gagatttact ctcgtgctcg
    16081 caaggggatt tccttagaaa tcaatgccct gtctgctcta gtctttctct ttagtattat
    16141 cctagttgta ggttattact ttatctctcg tgagaaggag gagcaagcat gaaaaaaatc
    16201 tattcatttt tagcaggaat tgcagcgatt atccttgtct tgtggggaat tgcgactcat
    16261 ttagatagta aaatcaatag tcgagatagt caaaaattgg ttatctataa ctggggagac
    16321 tatatcgatc ctgaactctt gactcagttt acagaagaaa caggaattca agttcagtac
    16381 gagacttttg actccaacga agccatgtac actaagataa agcagggtgg aacgacctac
    16441 gatattgcca ttccaagtga atacatgatt aacaagatga aggacgaaga cctcttggtt
    16501 ccgcttgatt attcaaaaat tgaaggaatc gaaaatatcg gaccagagtt tctcaaccag
    16561 tcctttgacc caggtaataa attctccatc ccttacttct ggggaacctt aggaattgtc
    16621 tacaacgaaa ccatggtaga tgaagtgcct gagcattggg atgacctttg gaagccggag
    16681 tataagaatt ctatcatgct ctttgatggg gcgcgtgagg tgctgggact aggactcaat
    16741 tccctcggct acagcctcaa ctccaaggat ctgcagcagt tggaagagac agtggataag
    16801 ctctacaaac tgactccaaa tatcaaggct atcgttgcgg acgagatgaa gggctatatg
    16861 attcagaata atgttgcaat cggcgtgacc ttctctggtg aagccagcca aatgttagaa
    16921 aaaaatgaaa atctacgtta tgtggtaccg acagaggcca gcaatctttg gtttgacaat
    16981 atggtcattc ccaaaacagt taaaaaccaa aactcagctt atgcctttat caactttatg
    17041 ttgaaacctg aaaatgctct ccaaaatgcg gagtatgtcg gctattcaac accaaaccta
    17101 ccagcgaagg aattgctccc agaggaaaca aaggaagata aggccttcta tcccgatgtt
    17161 gaaaccatga aacacctaga agtttatgag aaatttgacc ataaatggac agggaaatat
    17221 agcgacctct tcctacagtt taaaatgtat cggaagtagg agataaacgt aagaagacga
    17281 atcagtcaag ctggttcgtt tttaaggaat agaagtcagt ttttagaacc ctatcttttt
    17341 ctattctagt tgtttatttg tttatttgct tatttgcttg cctagtagta tactaagcct
    17401 atactcttca aaccacgtca gcgttgcctt gccgtactca agtacagctt gaggctagct
    17461 tcctagtttg ctttttgatt tttattgagt attaacctta cagaaagcgg caacaaataa
    17521 tgaaaatgtc tacttttttc aaaaaaagtt tctggccaac ctttatgatt gttaaccaaa
    17581 ctgctatact attccattta aaggatggct tggatcgaca atacctaaca actgagtcca
    17641 tatattgggt gataggcacc tttatatttg gaaatattct agtagctgtg tttagtaata
    17701 tgaaaatttg ggataaaaag aaaaatggca gtaagaaaaa atatatcctg aaaaagtaat
    17761 cagagatgta gggaattggt ctaattcaac ggagatcatt tccagttgac gcaagattaa
    17821 aaaaacgata taataagaaa gttgagaatt gattttcact tgtcttagtc cagagaaatg
    17881 gcggtgctgc gagccatcta agctaggaag tcatgctact caatcataca attgcataag
    17941 aacaagagaa tgacaagttc attgaatgaa ggtggtaccg cggtttttcg cccttcgtga
    18001 tatgagcttg tcttttgatt tttggaggtg ttgatgaaaa catttcttgt caaacaaaag
    18061 tttcgtcttg gaggcgaacg cttcgctatc aagaatgaca ggggagaaat tgcctatcag
    18121 gtggagggat cattttttaa gattcctaaa acctttacca tctatgatgc ggctggtgaa
    18181 caagtcagtg agattagtaa agaaatcttg accttgcttc ctcgttttga gattcagctt
    18241 cgggatggct cgagttttgt cattcgtaag aagttgacct tctggcgaga taagtatgag
    18301 tttgataatc taggtcttcg tatcgagggc aatatctggg atttgaattt caaattgctg
    18361 gatgatcgcg atcagctgat tgcggaaatt aagaaggaac tcttccatct gacctctacc
    18421 tataccgtaa cggttcttga ggacgcttat gcagacctag tcatttccct ctgtgtcgcg
    18481 attgactatg tggaaatgct ggaaagccaa tcacattaaa caagtaaata aggagacaat
    18541 atgaaacaac tatctagtgc acaagtacgc caaatgtggc ttgatttctg ggcgaccaaa
    18601 gctcactcag tagaaccatc agtaagtttg gttcctgtaa atgacccaac tcttttgtgg
    18661 atcaactctg gggtagcaac ccttaagaaa tactttgacg ggaccattat ccctgaaaat
    18721 ccacgtatta ccaatgccca aaaggctatc cgtaccaacg acatcgaaaa cgtagggaag
    18781 actgcgcgtc accataccat gtttgaaatg ctggggaact tctctatcgg ggattacttc
    18841 cgtgacgaag ctatcacttg ggcttatgag cttttgacaa gccctgaatg gtttgatttc
    18901 cctgctgaaa aactttacat gacctactat ccagacgata aagattctta caaccgctgg
    18961 attgaagtgg gagtggatcc aagtcacttg attccaattg aggacaattt ctgggaaatc
    19021 ggtgcgggac cttctggacc agatacagaa atcttctttg accgtgggga agcctttgac
    19081 ccagaaaata tcggtcttcg cctgcttgca gaagatattg aaaacgaccg ttatattgaa
    19141 atctggaaca tcgttttgtc acaatttaac gcagaccctg ctgttcctcg tagcgaatac
    19201 caggaattgc cacataagaa cattgatacg ggcgctggtt tggagcgttt ggtggctgtt
    19261 atccaagggg ctaagaccaa ctttgaaacg gacctcttca tgccgattat ccgtgaagtc
    19321 gagaaattgt ctggtaaggt ttatgaccaa gatggcgaca acatgagctt caaggttatc
    19381 gcagaccaca ttcgttctct ttcatttgct atcggtgatg gtgcccttcc aggaaatgaa
    19441 ggtcgtggtt atgttcttcg tcgtcttctc cgtcgtgctt ctatgcatgg tcaaaaattg
    19501 ggtatcaacg agcctttcct ttacaaactt gttccaactg ttggaaaaat catggaaagc
    19561 tactacccag aagtgcttga gaaacgtgac tttattgaga aaatcgttaa gagcgaagaa
    19621 gaatcatttg cccgtaccct tcactcgggt caacactttg cccaaggcat tgtagcagac
    19681 ttgaaagaaa aaggtcaatc tgttatcgct ggtcaagatg tctttaaact ctatgataca
    19741 tacggattcc cagttgaatt gactgaagaa attgctgaag aagctggtat gactgtagac
    19801 cgtgaaggtt ttgaagcagc catgaaagaa cagcaagaac gcgcgcgtgc gtcagctgtc
    19861 aagggtggct caatgggtat gcaaaatgaa actcttcaaa acatcactgt agaaagtgtc
    19921 ttcaactaca atactagcca attgtcttct aaattggtag ctatcgttgc tgacaatgca
    19981 gaagtaggag ctgtttcaga aggaactgcc tctcttatct ttgcggaaac gtcattttat
    20041 gctgaaatgg gtggacaggt agctgactac ggacaaatct tggatgagtc aggtaagatt
    20101 gtggctactg tgaccaatgt tcagaaagcc ccaaatggtc aagcccttca tacagttgaa
    20161 gtccttgcac cgcttgcctt gaaccaagaa tataccttgg caattgatag caatcgccgt
    20221 caccgtgtca tgaaaaacca cactgcgact catttgcttc acgctgccct tcacaatatc
    20281 cttggaaacc acgcaacaca ggcaggatct cttaacgaag ttgaattcct tcgctttgac
    20341 tttacccact tccaagcagt aactgctgag gaacttcgtg cgattgaaca gcaagttaac
    20401 gagaaaattt gggaagcact tgaagtgaag acagttgaaa cggatattga cactgctaaa
    20461 gaaatgggag ctatggccct ctttggtgag aaatacggca aggaagttcg tgtcgtgact
    20521 atcggtgact actctattga actttgtggt ggtactcatg ttggcaacac ttctgagatt
    20581 ggtctcttca aaattgtcaa agaagaagga atcggttcag gaactcgccg tatcttggca
    20641 gtgactggta aggaagcctt tgaagcctat cgtgaacaag aggatgctct taaagctgtc
    20701 gcagcaacct tgaaagcacc tcaagtcaag gaagtacctc acaaggtaga aggacttcaa
    20761 gaacaacttc gtcaacttca aaaagaaaat gctgagttga aagaaaaagc cgcagctgca
    20821 gccgcaggcg atatcttcaa agatgctaag gaagtcaacg gtcgtcgtta cattgctagt
    20881 caagtgtctg tatccgatgc cggtgccctt cgtacttttg cagataactg gaaacaaaaa
    20941 gactactctg atcttcttgt cctagttgcc gctatcggtg acaaagtcaa tgtccttgta
    21001 gcaagcaaga caaaagacct tcatgcagga aaccttgtca aagaattagc accaatcatc
    21061 gatggacgtg gtggtggtaa accagacatg gccatggcag gaggaagcaa ccaagctaag
    21121 atccaagaat tgttggatgc agtagcaggt aaattgtaat caagtaaaga tctatccatt
    21181 tgggtaggtc tttttgtgaa tacaaaaaag ccaaatccgc ttggacctag cttggttatt
    21241 atgagctatt agatggtatt gactgcccag acacttacgg atctagctga gacagggaat
    21301 tgtccataac cttcctcatc aattgtaact tgaccttggt ggttaccaag taaatctaca
    21361 aaggtttgat tagtccattc ttgaccgaca aacattgact tgctgttttc ttggtcattt
    21421 gagataagga ctgcgattgg ggattgattt tcagcacctg aacgtaccca accgatacag
    21481 ttagcatggt caaagtagtc attttgttct ccataggcca agtcttttcg gatggctagg
    21541 aggcggtcaa ggatttcttt gaaatcttct tgagcatact ggcctgaaat cccatagtag
    21601 tctccgtaaa agacacatgg aaggccgtct tggcgtaaca aaatgagggc ataggctgct
    21661 ggcttgaacc attcttcaac ggtagactca agggcttgtc ctcgttgggt atcgtggttg
    21721 tcgacaaaag tcacagcctt gtcaggcttg agttcaacca ggctatctgt gaaaatgcca
    21781 cgaaggtcat agtttgcgcc agcttgactg gcttcaaaga gattctggtg gagacgaaca
    21841 tcgacaaggt caaagtgttc ttccgttttt tcgagatagt ccagattggc ttccttgtct
    21901 gggttccaaa attcaccaaa aacatagaaa tcgtcaccgt atttttcctt catatcgcgg
    21961 atgaagttgc gcataaagaa agagtcaata tgcttaacgg catccaaacg gaaaccagct
    22021 acaccagtcg tttccatgaa ccaatcagcc cagtcataga tgttttggat gacttcagga
    22081 tgtttaaagt ctaggtcggc atacatgagg tagtcgtagt ttccgttttc gttatcgacc
    22141 aattcctcgt tagcccagcc cttgttgtcc ccttggatca gataaatccc agatttactg
    22201 cgtttggcat cgtagtctgt accggtgaag tggtaccaat gccagtggaa gccattatag
    22261 gtatcttggc gaccatcgaa ggtaaaacta gtccagccat tgatggtgaa gggttctcca
    22321 agttcaactg tacggtctac aggatcaact tcgataacct gaaaggcttc cctgttatcg
    22381 gcagcagcct tgtggttgag aactacatcg gccataggtt gaattccctg ttctttaagg
    22441 gcttgaatgg cttgaagata gtcttctttg aaaccatact tggtgcggac agtccctttt
    22501 tggttgaact ctcctaagtc aaataagtca tagaccccat agccgacatc tttttcgttg
    22561 gttgccttga aggctggtgg catccagacg tgactgatcc ccagatgagc taggtgtgga
    22621 gcattttcag ccagacgcgt ccagtgttga ccgtcgtggg gcagatacca ttcaaagtat
    22681 tgcatgagtg tttgattttg catgatgttt cctcttactt gtcaatcttg ttctttattc
    22741 tatcataaag tatctctcgg cgcaaacgtt tgcgtaagat agaaggaaac tattttaact
    22801 acttaaaata aagtattgat ttttttgtat caaagtgcta gtatagcgat atataataca
    22861 gataaagata ggagttgtcc catgattgaa tttcaaaatg ttagtaagtt gtatggtgat
    22921 aaagaggcct tgagtaatct caatttgcag attgaaaatg gagagattat gggcttgatt
    22981 ggtcataatg gggctggaaa atcgaccact ataaaatccc tagtcagtat catttcaccc
    23041 agcagtggtc gtattttggt agacggtcag gagttatcgg aaaatcgctt ggctattaaa
    23101 cgaaagattg gctacgtagc agactcgcct gacttatttt tacgcttaac ggccaatgaa
    23161 ttttgggaat tgatcgcctc atcctatgat ctgagtagat ctgacttgga ggctagtcta
    23221 gctaggctat tgaacgtttt tgattttgct gaaaatcgct atcaggttat tgaaactctt
    23281 tctcacggaa tgcgtcagaa agtctttgtc atcggagcac tcttgtctga tcccgatatt
    23341 tgggtcttgg atgaaccctt gactggtttg gatccccagg ctgcctttga tttgaaacag
    23401 atgatgaagg aacatgcaca aaaagggaag acagtcttgt tttcaactca cgtcctagag
    23461 gtggcagagc aagtctgtga tcggattgcc attttgaaaa aggggcattt gatttattgt
    23521 ggtagtgtag aggacttgag aaaagattac ccagaccagt ctttggaaag tatctacctt
    23581 agtcttgctg gtagaaaaga ggaggttgcg gatgcgtctc aaggtcatta aaaaattagt
    23641 tgatatcaat atcctttatt catctcaaga agctaatctg gctaatctac gaaagaagca
    23701 ggctaagaat cctgggaaaa aagtaaatgt ttccgctaga gtcctaagtt cttacatttt
    23761 ttccagtctc ttgatgctca tctgttttag taatatagcc attcattttc cttttgagga
    23821 aatacctatt tattttagct cgatggttgc tattttacta gtgattgcct tttcaacttc
    23881 tttaacggcc ttttacaatg tcttttatga gagtaaggat ttggtatcgt ataggcccta
    23941 tgcttttaaa gaatcagaga ttataattgc taaaggactg tctgtcctct tgccagctct
    24001 aactggaatt gtaccaatcc tagcttattt tctagtcctc tacattaggc tagctccttc
    24061 tctatggttg ggtttgcctt tgatgctact gtccttgacc ttattatttg tctctgtggc
    24121 tctagtgatg gtagtggcag tgcatttctt ggctcaaact agggtcttca gaaagtatca
    24181 gtctattttt tcgaatgtga tgattgggat aggagttctc atacctttaa tatttgtctt
    24241 ctttctacag tcgacttttg gaagtattgt tgacaaagtt agagacattc catttctcct
    24301 ttatcctctt catatctttt acaaaatagc agtggagcct ttttcaatag aagccatatt
    24361 ggatctgctc gcttggatag ctttaactgt tttcttgctt tacctgacca aaaagaaggt
    24421 ccttcctcgt ttttatgacg tgatcctgct taacagtgag gagaaggtca aaaaagaacg
    24481 tcgcagcaag gagaggattt caactactaa aaagggcttt ttccgtatgg ttttacgcta
    24541 ccacctcacc ctcttgggac aggggactgg cgtgattaca gtgcttttta caagtgcttt
    24601 ccttccttat ctcatgatga tcagtctgat ttccaaaatc cgagattctc agatagttcc
    24661 agacattcat cctccatact ggttaccctt gttttttgta ggagtgttta tagcagttgt
    24721 caataacaat atcaccagcc tgcattcaat tgccttgtcc ttggagaggg aaaatgttga
    24781 ttttctgaag agtttaccct ttgactttgc tcgttatgtg aaagtgaaat tttggattat
    24841 ctttgctgtt cagtcctttt taccaattct gactttgctc ggtctttctc tatatctagg
    24901 cttgtccatc ctttcgatga tttaccttat tgtggcatgg atccttgcca gtgtcatcct
    24961 ttcttgccac cattacttta aggacgttaa aaatctgtca accaattgga gtagcattac
    25021 ggacctggtg aatcgttcaa atggcatagt cgccatagtt ttattgttta tttatagtgc
    25081 aatcctgatg gcccttgtaa ttgggagcat attcttggtt cagtctctct cccctatcct
    25141 tgccatcagc ttgggagtag gagctcttat cctcctgctt gctcttgcta tttttggcta
    25201 tcattattac ctgtcacgca tattggcaga aatagaaaaa agatgaactg gttggtcgct
    25261 tgcttgataa attcaattcg acaaacgctt ttatagtttg ttagaatgta gttgtaaatg
    25321 attaagattg gtaatcaaaa atatagggag gaatgttttg aataagaaat ataacatagt
    25381 tctatttttg ttgtttatag tttatttatt gggatatttt tcaatttcaa aaacgttaat
    25441 ccctataatg tgtgttccaa gtatttttga tagaacatat atttagagtt cgaaatagaa
    25501 tgatgcagat aggtgaaatt ataattattg ttgcttctat aatattattt attgatagta
    25561 tattgagttt gtaacaaaag gcttctctat aaaatcaaat tttccagata cctactgaag
    25621 ctgatttatt tctttatctt tcttctacta cctttgtcac taaatttgac agacacatat
    25681 tgctgataca acctctctac ctattaatct cacagttaac ctgaggctag tttcatagtt
    25741 tgctatttga ttttcgttga taaaaaaaat gctcctccaa gacggaagag cattttttat
    25801 tgtgattctt acacatttac ttcctagttt tgcaggaagt ctagtatacc attttttaaa
    25861 agaagagtaa aaaagcaagg gtagtttctt ttttgagaat tgtctaaaag tttgatataa
    25921 tggtagttat gaatagaata agagtcagca aaagggttga aaagaagctt gctaaggggc
    25981 tagttttact agaagccagt gatcttgaga atgtcaatct taaggatcag gaagtagagg
    26041 tgcagggtca ggaaggaaac tttcttggga ctgcctacct ttctcagcaa aacaagggct
    26101 tgggctggtt tgtcagcaaa gacaaggtgg ccttcaatca agctttcttt gaaacgttgt
    26161 ttagaaaagc caaagaaaag agaagcgcct actatcaaga cgatttaaca actgcctatc
    26221 gtctctttaa tcaagaggga gatggctttg ggggtctgac agtggacctt tatggcgact
    26281 acgccgtctt ttcttggtat aactcttatg tttatcagat tcgtcagact atatcagaag
    26341 cctttagaca ggttttccct gaggttttag gggcttatga gaaaatccgc tttaagggtt
    26401 tggactatga atctgcccat gtttatggtc aagaagcacc tgactttttc actgttttag
    26461 aaaatggtgt cctgtatcaa gtctttatga atgatggctt gatgacaggc attttcctag
    26521 accagcatga ggttcgcggt agtttagttg acggcttggc tatgggtaaa tccttgatca
    26581 atatgttttc ctacacagcg gctttttcag tagctgcggc catgggagga gctagccaga
    26641 caacttctgt tgatctagcc aaacgttcac gagaattgtc tcaagcgcat tttcaggcaa
    26701 atgggctcag cacagacgag catcatttta tagttatgga tgtctttgag tatttcaaat
    26761 atgccaaacg caaagacttg acctacgatg tgattgtcct agatccgcct agctttgctc
    26821 ggaataaaaa acaaactttc tctgtggcca aggattatca caagttgatt tcccagagtc
    26881 ttgagatttt aaatccggga gggattatca ttgccagtac caatgctgcc aatgtttccc
    26941 gtcagaaatt tacagaacaa attgataaag gctttgcagg aagaagttac cagattttaa
    27001 acaaatatgg tcttccagca gattttgcct ataataaaaa agatgaaagt agtaattacc
    27061 tcaaggtgat tagtatgaag gttagtaaat gaaattaatc gtttcagtaa tgccaagaag
    27121 tttagaggag gctcaggctc tggatgccac gaggtacctg gatgccgaca tcattgaatg
    27181 gcgtgccgac tatctgccta aagaagcgat tttgcaggta gctccagcca tttttgaaaa
    27241 attcgcaggc cgtgagttgg ttttcacgct acgaactcgc tccgaaggtg gagaaatcga
    27301 cctttctcca gaagagtata tccatctaat caaggaagtt gcgcaactct atcaaccaga
    27361 ctatattgat tttgagtact atagctacaa ggatgttttt gaggaaatgc tggacttccc
    27421 aaatcttgtt ttgagttacc ataatttcca agaaacacct gagaacatga tggaaatctt
    27481 gtcagagttg acgatcctaa atccaaaact tgttaaggtt gcggtgatgg ctcacacgga
    27541 gcaggatgtc ttagacttga tgaactatac acgaggcttt aaaaccctca atcctgaaca
    27601 ggaatatgtg accatttcta tgggtaaggt gggcaaggtc tctcgtatca ctgcggatgt
    27661 gactggttcg agttggtctt ttgccagtct ggatgaggct agtgcccctg ggcagatttc
    27721 tctagctagc atgaaaaaaa tcagggagat tttggatgaa gcttgatggc tatacacgtt
    27781 taactgcagt tgttgccaat cctattaagc attctatttc tcccttcatc cacaatagag
    27841 cctttgaggc gacagctacc aacggtgctt atgtggcttg ggagattgaa gcgagtgact
    27901 tggcagaaac agtggccaat attcgtcgct accagatgtt tggcatcaat ctgtccatgc
    27961 cctataagga gcaggtgatt ccttatttgg atgagctgag cgatgaagcg cgcttgattg
    28021 gtgcggttaa tacggttgtc aatgagaatg gcaatttaat tggatataat acagatggca
    28081 agggattttt taagtgcttg ccttctttta caatttcagg taaaaagatg accctgctgg
    28141 gtgcaggtgg tgcggctaaa tcaatcttgg cacaggctat tttggatggc gtcagtcaga
    28201 tttcggtctt tgttcgttcc gtttctatgg aaaaaacaag accttaccta gacaagttac
    28261 aggagcagac aggctttaaa gtggatttgt gtgctttaga atatgtttct gaactgcaag
    28321 caaggattgc cgagtcggat ttgctagtta atgccaccag tgtgggcatg gatggccaat
    28381 cctctcctgt tcctgaaaac atagtcttac cagaaactct cttagttgta gatatcatat
    28441 accaaccctt tgaaacaccg tttttgaaat gggctagaag tcagggcaat ccagcagtca
    28501 atggtctggg aatgttgctc tatcaagctg cagaagcttt tcaactgtgg acaggcaagg
    28561 aaatgccgac agaagagatt tggcagtctt taacagaaaa ataccaataa agaaagggag
    28621 actctcctat gaaaatcaga atcgatattc ctcatcatcc ttatgatatt cagattgaaa
    28681 aaggttgtat ggcccaggct ggtcagtggt tgcgagaact ctggcaaccg caaaaggtag
    28741 tcattgtgac agataaccat gtagcctctc tctatgcaga gaaggtcaag ctcagcctag
    28801 aagatgctgg ttttcaggta gctgtttttg atttcttaga aggtgaagaa agaaagaatt
    28861 taactactgt tcagaaagtc tatgaatttc tagtcaagca aggtctgact cgtagcgatg
    28921 gaatcgttgc tcttggtggt ggcgttgttg gggacctggc tggctttgta gcctctacct
    28981 atatgcgggg tattcacttt gttcagattc cgactagttt gacagctcag gttgattctt
    29041 ctatcggtgg aaagacaggc gttaacacac catttgctaa aaatatggtg gggacctttg
    29101 ctcaaccaga tggggttctg attgatccac ttgttcttga aaccctcgga aaaagagagt
    29161 tgattgaagg gatgggtgaa gttatcaagt atggcttgat tgaggatcca gaactgtggg
    29221 ctctcttgac ggggctgaat ggttctgttg agagcatttt ggaacatgca gagaccttga
    29281 ttgaacattc ttgtcaggtg aagcgcaaga tggtggttga agatgagttg gacaatggta
    29341 ttcgtcttta cctcaatttt ggccacacta ttggccatgc catcgaagcg actgccggtt
    29401 atggcaaggt catgcatggg gaggctgttg ccatgggaat ggtacagatt tccaaggttg
    29461 ctgaggaaaa aggcctcatg ccagctggca ttacccaatc tatcacagag atgtgtcaga
    29521 aattcggctt gcctgttgac tatgaaaatt ggaaagttga caagctttat caggctctta
    29581 ctcatgacaa gaaagcgcgt ggtaacacct tgaaattggt tttggtgcca gagcttggtt
    29641 cagcgaccat tcacccagtt tctctggaag agatgaaaga ctacttggta aaataaggag
    29701 agtgtatgag atatttaact gcaggagaat cacacggccc ccgtctaaca gctattattg
    29761 agggaattcc agctggactt ccattgacag ctgaggatat caatgaggat cttagacgcc
    29821 gtcagggtgg ctacggtcgt ggtggtcgta tgaagattga gagtgaccag gttgtcttta
    29881 cttcgggtgt tcgccacggg aagacgacag gggcgcctat tactatggat gtcatcaata
    29941 aggaccacca gaaatggctg gacatcatgt ctgcggagga cattgaagac cgccttaaaa
    30001 gcaagcggaa aattactcat cctcgcccag gtcatgccga tttggttggg gggattaagt
    30061 accgttttga tgatttgcga aattctttgg agcgttcatc agctcgtgaa accaccatgc
    30121 gggtggcagt tggtgcagta gccaaacgcc tcttggctga gctggatatg gagattgcca
    30181 accatgtcgt ggtctttggt ggcaaggaaa tcgatgttcc tgaaaatctg acagtcgctg
    30241 aaattaagca aagagctgcc cagtctgaag tttctattgt caaccaagaa cgagaacagg
    30301 aaatcaagga ctatattgac caaatcaaac gtgatggtga taccatcggt ggggttgtgg
    30361 agacagtcgt cggaggcgtt ccagttggtc ttggttccta tgtccaatgg gatagaaaat
    30421 tggatgcaag attggctcaa gctgttgtct ctatcaatgc ctttaaaggg gtggaatttg
    30481 gtcttggctt tgaggctggt tatcgtaaag gcagccaagt tatggatgaa attctctggt
    30541 ctaaagaaga cggttatact cgccgtacca ataatctagg tggttttgaa ggtggtatga
    30601 ctaatgggca acccatcgtt gttcgtgggg tcatgaaacc cattcctact ctttataaac
    30661 ctcttatgag tgtggatatc gaaacccacg aaccttacaa ggcaaccgtg gagagaagtg
    30721 atccgactgc tcttccagct gcaggaatgg tcatggaagc agttgtagca acggttctgg
    30781 cgcaagaaat cctcgaaaaa ttctcatcag ataattttga ggaactaaaa gaagcggtag
    30841 ccaaacaccg agactataca aagaactatt aaggagttcc tatggcaaaa acaatctata
    30901 tcgcaggtct tgggttgatt ggagcctcta tggcacttgg tatcaaacgc gatcatccag
    30961 attatgaaat tttaggttat aatcgtagtc aagcttcgag agatatcgcc ttgaaagaag
    31021 gcatgattga ccgtgcaacg gatgattttg ctagttttgc tcctttggca gatgtcatta
    31081 tcctcagctt gccaatcaaa caaactattg ctttcattaa ggagttggcc aatttggatt
    31141 tgcgagaagg cgttattatt tcagatgctg gttcgaccaa gtcaaccatt gtggatgcgg
    31201 cggagcagta tttggctggc aagtctgttc gctttgtcgg ggcccatccc atggctggta
    31261 gtcacaagac aggggctgct tcggcagatg tcaatctttt tgaaaatgcc tattatatct
    31321 ttacaccttc aagcctgaca agtcaggaca cgcttaagga aatgaaggat ctgctttcag
    31381 gtcttcatgc tcgttttatc gagattgatg ccaaggagca tgatcgtgtc acttctcaga
    31441 ttagccattt tcctcatatt ttggcttcta gtctcatgga gcagactgcg gtctatgctc
    31501 aagagcatga gatggcaagg cgctttgcgg caggtggttt tcgagatatg acccgaattg
    31561 cggaaagcga gccaggaatg tggacctcca ttctcttgtc caatagcgag atcattctgg
    31621 atagaattga ggatttcaag gaacgtttgg aagcgattgg tcaggccatt agtaagggag
    31681 atgaagagca aatttggaac ttttttaacc aagcgcgtga acaacgccag accatggaaa
    31741 tccataagcg tggtggtgtg gatagctctt acgacctcta tgttgacgtt cctgatgaag
    31801 aagatgtcat cctgcggatt ttggaactgc tacgtggaac ttccttggtt aacatccaca
    31861 tcaacgagga aaatcgtgag gatattcacg gaatcctaca aatttcattt aaaaatgctc
    31921 aagacttgga aagagctgaa tatctcataa cagaaaatac cgactataca gtcgtcatca
    31981 aataaggaga agaacatgtc aaatatttac gatagtgcaa acgaactcag tcgcggtcta
    32041 cgcggattac cagaatacaa ggctgttaaa gcagctaaag atgcgattgc agcagatgct
    32101 gaggcaagca aaatctttac agaatatctt gcctttcaag aggaaattca aaaactagca
    32161 cagacaggtc aaatgccaga tgcgtccttc caagctaaga tggaaggctt tggtaaacag
    32221 attcaaggaa atagcctctt gtcagaattc tttaccaagc aacaacaatt ggccatttac
    32281 ctttctgaca ttgaaaaaat tgttttcgaa ccagtttcag aattgctgaa ataagaaaag
    32341 aacgatccta aagtcaggaa tttttaagga atttctggct ttttatgata aaataggtaa
    32401 aagtattgca agtatgaggt ccagtatgaa actaaaaaca aacattcgcc atttacatgg
    32461 tagtatccgc gtcccaggtg acaagtctat cagccaccgt tccattatct ttggaagttt
    32521 ggctgagggt gagaccaagg tttatgatat tctgcgaggt gaagacgttc tttcgaccat
    32581 gcaggttttt cgtgaccttg gtgttgaaat tgaggataaa gatggggtta ttaccattca
    32641 aggtgtaggc atggctggct taaaagcgcc gcaaaatgcc cttaatatgg gaaattctgg
    32701 cacctcgatt cgcctgattt caggtgtcct tgctggtgca gatttcgaag tagagatgtt
    32761 tggagatgat agtctttcca aacgtcctat ggaccgtgtg acccttccac tgaaaaaaat
    32821 gggcgtcagc atctcagggc aaactgaacg agacttgcct ccccttcgct taaaagggac
    32881 gaaaaaccta agacctattc attatgagtt gccaattgcc tctgcccaag tcaagtcagc
    32941 cttgatgttt gcagccttac aagctaaggg ggagtcagtt attatcgaaa aagaatacac
    33001 ccgtaatcat actgaagata tgttgcaaca atttggtggt catttaagtg tggacggtaa
    33061 gaaaatcaca gtccaagggc cacaaaaatt gacaggacag aaggtggtcg taccaggaga
    33121 tatttccagt gcagcctttt ggttagtcgc aggtttgatt gctccaaatt ctcgtctagt
    33181 gctgcagaat gtggggatca acgaaactcg caccggtatt attgatgtca ttcgtgccat
    33241 gggtggaaaa ttggaaataa ctgaaatcga tccagtcgct aaatctgcaa ccttgattgt
    33301 tgagtcttct gacttgaaag gaacagagat tggtggcgct ttgattccac gtttgattga
    33361 tgaattgcct attattgccc tacttgcgac ccaagcccaa ggtgtaacag ttattaaaga
    33421 tgctgaggag ctcaaggtca aggaaacaga ccgtattcag gttgtggcag acgccttaaa
    33481 tagtatggga gcagatatta ctcctacggc agatgggatg attatcaaag gaaaatcagc
    33541 tcttcacggt gctagagtca atacgtttgg tgaccaccgt atcggcatga tgacagctat
    33601 cgcagccctc ttggttgcag atggagaggt ggagcttgac cgtgcagaag ccatcaatac
    33661 cagctatcct agtttctttg atgatttgga gagcttgatt catggctaag gtgttattag
    33721 ggtttatggg ggctggaaaa tcgactattg caagaggctt ggaccctaat taccttgata
    33781 tggatgctct gattgagaat cgcctaggta tgtccattgc gaattttttc gctgaaaagg
    33841 gagaaacagc ctttcgtcag gtagaatcag aagtcctagc tgatttacta caaacagacc
    33901 aagtcgtgtc aactggagga ggagtggtta tttctcagag aaatcgtgac ttactcaaga
    33961 ctaatacaga taacatctac ctgagagcag attttgaaac cctctaccaa cgtatcgcag
    34021 ctgataagga caatcagcga ccgctttttc taaataatag caaggaagaa ctagtagcta
    34081 ttttccaaga aagacaggct tggtatgagg aagtggctag tcgggttttg gatgtgacca
    34141 agctaagccc agaggaaatt atagaggaac taagatgaaa attgcttatc taggtcccaa
    34201 gggatcattt tcacaccacg ttgtgcagac agcttttcct catgaggaat tgcaggcttt
    34261 tgccaacatt acagatgtca tcaaggctta tgagcaagga ttagtgaact attctgtggt
    34321 gccagttgaa aattctattg agggtagtgt tcatgaaacc ttggactatc tttttcatca
    34381 ggctcacatc caagcagtag cagaaatcgt tcagcctatt catcagcagt tgatggtggt
    34441 tccaggccat actaagattg aaaagatttt ttcacatcca caggccttgg ctcaaggaaa
    34501 gaaattcatc gatgaacaat atccagaggc tcaaatcgag gtaacagcta gtacagctta
    34561 tgcggcccgt tttatttccg aacatccaga ccagcccttt gcagcagttg cacctagaag
    34621 ttctgctgaa gaatatggat tggaactgat tgccgaggat attcaggaaa tggaagccaa
    34681 tttcacacgt ttctgggttc taggagctga aaagcctagt attcccttgc aagcacaaac
    34741 tgaaaagatg agtttggcct tgacattacc tgacaacctt ccaggtgcac tttataaggc
    34801 cctgtcgacc tttgcttggc gaggaattga cttgacaaaa attgaaagtc gtccactcaa
    34861 gacagcactg ggtgaatact ttttcattat cgatgtggat tataccgata aggacttggt
    34921 ccactttgcc caaaaagaat tagaagcgat tggaatccag tataaaattc tgggtgccta
    34981 tcctatttat ccaatatcag accatggaaa ggagagaaga tgagtaaaga aaatccctta
    35041 agtcatcatg agcagttgcg ttatgattat ttgctaaaaa atattcacta tctcaatgag
    35101 agagaaaaaa atgagtttgc ctatttgcaa gaaaagctaa ctcttgctag gggaaatagt
    35161 agctctagct tggaacaaga aagagaagag caggttgact taccaagcta tgcgaaccgg
    35221 agtcgctcac aatccaaatc acaagcactc tctttccctc caaaaaagaa aagacggaag
    35281 ctccgtctta aacgaatttt tatggtgatt ttttcacttt tggtctgtgt ggctttggcc
    35341 atggtattca tgtttttgcg tggttaccaa gatgctagcg caaagaaaac tgctgatgcc
    35401 cgggcagctc aagtagaagt ctttaatggt caggacacta gagatggagt taatatttta
    35461 atcatgggta ctgatggtcg aatcggccag aacagtgttg agacacgaac tgactctatt
    35521 atggtattaa atgtcggggg ctcagataag aaaatgaagc tagtcagttt catgcgtgat
    35581 aatttggtct atatagatgg ttatagtcaa gtgattaacg gtagaaaaca gacagataac
    35641 aagttaaacg tagcctacga gttaggagaa caagaggggc aaaaaggggc agaaatggtt
    35701 cgccaagtct tgaaagataa ttttgacttg gacattaagt actacgcctt ggttgatttc
    35761 caggcctttg ccacagcgat tgacacgctt ttccctgatg gggtgacgat tgatgctcaa
    35821 ttttcaacat tgaatggtcg tcccctaaca gaagctacag tcggagatga tttatacgct
    35881 accgagactg agtctccaac ccaaaccatt aaagtcggaa aacagcagat gaatggctcg
    35941 accctgctca attatgctcg tttccgtgat gatgatgagg cggattacgg ccgtacaaaa
    36001 agacagcaac aagttttaac agcaatttta gagcaaatta aagatcccac taaacttttc
    36061 actggttctg aagctcttgg aaaggttttt gctatgacct caaccaacgt accctatact
    36121 ttcctcctaa caaacggttt atctgttttg gatggagcaa aaaatggtat tgaaaaattg
    36181 acgattccag aactgggtga ctgggtagat gcctatgatg tttatggagg cttgggcttg
    36241 ctggttgatc aaaacaaata tcaaaccaag ctcgctcaaa tgggcttgag ataagataat
    36301 actaaatgaa aatcaaagtg caaactagga agctagccgc aggctgctaa aaacacagtt
    36361 ttgaggttgt agataagact gacgaagtca gttacctata cctacggcaa ggtgaagctg
    36421 acgtggtttg aagagatttt cgaagagtat aagacagaaa aatcaaagtt tgatagctat
    36481 ttggatagca gtcaggcttt gattttttac agtctgcaat agattttcac cccttgtttg
    36541 tggtataatg aaacgatacg atagaaagaa taatgaacaa tatgactgat ttaaaagcaa
    36601 ttcaggctcg tagtctggag atggctgaat attttgtggc cttttgtaaa gaacatgatt
    36661 tgctctgtta tctctgtggc ggaggcgcta ttggtgccct tcgaaacaag ggatttattc
    36721 cttgggacga cgacctagac ttttttatgc ctcgtaaaga ttatgagaaa ttagcagaat
    36781 tatggcctcg ttatgcagat gaacgttatt tcttgtcaaa gagtcacaag aattttgttg
    36841 atcgtaatct ttttattacc attcgtgaca aggaaaccac ctgtatcaag ccttatcagc
    36901 aggatttgga tttgccacat ggtctggcct tggatgtttt gcctttggat tattatccga
    36961 aaaatccagc tgagcggaaa aaacaggttc gttgggcctt gatttattca ctcttttgtg
    37021 cgcaaactat tccagaaaag catggtgctc tcatgaaatg gggaagtcgc attttactgg
    37081 gtttgactcc aaaatctctc cgttatcgca tctggaaaaa agctgagaaa gaaatgacta
    37141 agtatgattt ggctgattgt gatggcatta cagaattatg ctcaggtcct ggctacatga
    37201 gaaacaagta cccaatcaca tcttttgaat acaatctttt cttgccattt gaaggaacag
    37261 agatgcctat tccaatcggc tatgatgtct atctcagaac tgcttttggg gattatatga
    37321 cgcctccacc agcagacaag caggtaccgc atcatgatgc tgtcatcgct gatatggata
    37381 agtcttatac agaatacaag ggagaatatg gtggctaaga aaaaaatctt attttttatg
    37441 tggtcttttt ctcttggagg tggtgcagag aagattctat caaccattgt ttcaaatctg
    37501 gatccagaaa agtatgatat tgatattctt gaaatggagc actttgacaa gggatatgaa
    37561 tctgttccaa agcatgtacg cattttaaaa tcccttcaag attatcgcca aactagatgg
    37621 ttacgagctt ttttgtggag aatgagaatt tattttccaa gactggctcg tcgtttgctt
    37681 gtaaaagatg attatgatgt tgaagtttct tttaccatta tgaatccacc actgttgttc
    37741 tctaaaagaa gagaagtcaa gaagatatct tggattcatg gaagtattga agaatttctt
    37801 aaggatagct ctaaaagaga atcacataga agccagttgg atgctgcgaa tacaattgta
    37861 gggatttcaa aaaaaaccag caattctatc aaggaagttt atccagatta tgcttctaaa
    37921 ttacagacaa tctacaatgg atatgatttt cagactattc tagaaaaatc tcaagagaag
    37981 atcgatatcg agattgctcc tcaaagtatc tgtactatcg gacggattga ggaaaataag
    38041 ggttctgacc gtgtagtgga agtgatacga ttattacacc aagagggaaa aaactatcat
    38101 ctctatttta tcggggctgg tgatatggaa gaggaactga aaaaacgagt caaagagtat
    38161 gggattgagg actatgtaca tttccttggt tatcaaaaaa atccttatca gtatctatct
    38221 cagacgaaag ttctcttgtc tatgtctaaa caagaaggtt ttcctggagt gtatgtggag
    38281 gccttgagtc tgggactccc ttttatctct acggacgttg gaggggctga ggaattatcc
    38341 caagaaggac gatttggaca aatcattgag agcaatcaag aggcagctca ggcgattact
    38401 aattacatga cttctgcctc aaactttgat gtcgatgagg ctagccaatt cattcaacaa
    38461 tttacaatta caaaacaaat cgaacaagta gaaaaactat tagaggagta gcatggaaac
    38521 tgcattaatt agtgtgattg tgccagtcta taatgtggcg cagtacctag aaaaatcgat
    38581 agattccatt cagaagcaga cctatcaaaa tctggaaatt attcttgttg atgatggtgc
    38641 aacagatgaa agtggtcgct tgtgtgattc aatcgctgaa caagatgaca gggtgtcagt
    38701 gcttcataaa aagaacgaag gattgtcgca agcacgaaat gatgggatga agcaggctca
    38761 cggggattat ctgattttta ttgactcaga tgattatatc catccagaaa tgattcagag
    38821 cttatatgag caattagttc aagaagatgc ggatgtttcg agctgtggtg tcatgaatgt
    38881 ctatgctaat gatgaaagcc cacagtcagc caatcaggat gactattttg tctgtgattc
    38941 tcaaacattt ctaaaggaat acctcatagg tgaaaaaata cctgggacga tttgcaataa
    39001 gctaatcaag agacagattg caactgccct atcctttcct aaggggttga tttacgaaga
    39061 tgcctattac cattttgatt taatcaagtt ggccaagaag tatgtggtta atactaaacc
    39121 ctattattac tatttccata gaggggatag tattacgacc aaaccctatg cagagaagga
    39181 tttagcctat attgatatct accaaaagtt ttataatgaa gttgtgaaaa actatcctga
    39241 cttgaaagag gtcgcttttt tcagattggc ctatgcctac ttctttattc tggataagat
    39301 gttgctagat gatcagtata aacagtttaa agcctattct cagattcatc gttttttaaa
    39361 aggccatgcc tttgctattt ctaggaatcc aattttccgt aaggggagaa gaattagtgc
    39421 tttggcccta ttcataaata tttccttata tcgattctta ttactgaaaa atattgaaaa
    39481 atctaaaaaa ttacattaga acgggggtga ccaagtgatt gatgggaaac gattattatt
    39541 tagtttgacc atagtcagtt atgccttgac gctagtaagt ggaattgtgt atctgtttaa
    39601 taataataat gttagcttac tttctacttt attgttcttg ttggttagta gcttaattgc
    39661 ttgttggaat gatatcaagt attacttaat ccattttatt ttctatttaa ccatttttgt
    39721 atttctggta tcaagaccga ccattgatta ttttagggat ggtgctttgg atacctatca
    39781 tccaatagcc tatcgttttg cctttatagt tgtcatggtt tcgattctgg gcttgaccac
    39841 aggaggcatt ctggctcgtt acttcatagc taggaagaaa ataaaagtag caaatatagg
    39901 aaattctcta aaagaggttt atatcaagcg gttacgcttt gtatcactag gagtttttct
    39961 tctaacctat cctttctatt tcattcggtt atttgaacgg ctcttgtatc gtttgcagac
    40021 ttcctactat gcctactatg caaattttga aagtaaactg ccttatttta cctacatttt
    40081 gtctaccttt acggtctatg caatgtgtat gtatctggca accaagccaa agaaattgca
    40141 ggccacagca gtgcttgtct cctttattgc agctaatact attcatttgg caattgggac
    40201 acgaaatccc tttattttaa gtattttatt tgcttttgtt tattacttta tgcgggagca
    40261 aactgaaaaa ggaaaatgga ttgggtttaa agaaaagtta gcgatttttg taggttctcc
    40321 tattctcatg ttagcgatgg gagtactcaa ttatgtacgg gataatgtcc aagtttccca
    40381 tacaggtttc tgggatatct tacttgactt tatctataaa caagggacta gttttggtgt
    40441 tctggctcga ggttttctat ttaacagtag cctcccttac cgagatttcc gtaattttac
    40501 ttttggtcct gttcttgatt attttgcaag ggggagtttg ggagccattt tcggaggaaa
    40561 agcctttgaa catacaacca atagtgtgga actagctatt gatagtaata gttatgccca
    40621 caatctatcc tatcttgtct tgaacaagga atacttgaaa gggcatggta tcggaagtag
    40681 ttatatcatg gagttgtata ccgactatgg tatgattgga gtctttctgc ttagtttctt
    40741 actcggcgta ttatttatag ccatgctgca agtagcctat cgctcaagga caatcctatt
    40801 tgctttatcc ctactcatct tgaataatct attctttatg ccaagaagca gcttttcaga
    40861 aagtttcttc aatttattta caatgcaatt ctggggaatt gttcttgtga ttatatttgt
    40921 agcaaaaatg cttacaaagg aaaactagta tctactaaac aaaggagaaa aaaatcatgt
    40981 ttgaacatta ttcagtagct gatttgtttg caaatcttta taaaaaacga aaagcaaata
    41041 ttttagctct catcgcttta tttgctctca ttgctgtacc atttacaatt aaagcagtta
    41101 ggaataaaaa cactgtcaaa gatacaacaa gttattcaac ttatcttatc tataaaatca
    41161 ctcctccaga agattcggcc aaaacgattt tgaatcatca aattggtggt tatagtgatt
    41221 tttatgggaa attgattgat ggtaatttga atggagctta tcttttcaat gatgtagaac
    41281 ccagtgagtt gaaaaaaatt gccagtgaat tagacacgac ggaaacaacc ttgaaaaatt
    41341 caacgaatga ctattggtgg aaaaaattga ccgtctacta tatgattgac gatgcagggg
    41401 ttggtgtgaa aattttgaca tcaagtaaag atgccaatga cttgttagag aaaaaaattg
    41461 atgggttgat tgagaaattt aaacatgctt atgcaaatgt gaaaattgaa aaactggaaa
    41521 ccatcaactc taaagaattg aacgcaaatg gtgaaacagc gcttggctta aatgtgaaaa
    41581 atctgattct tcgtttagtt gttattggag tggtttgtgt gattttggtt gtgatgggaa
    41641 atgtgttagt ttatctcttt aatccaacaa tcaatagagt aggtgatttt tctcagtatc
    41701 aaattgattt tgtaacagag attacaacaa ttgctaacct agcagatgtt ttgtcataca
    41761 aaaatactgg acaggaattg accatcgtta gctcaaataa agctatccta gataaattga
    41821 aacagagtca agaagcttta aaaggaatgc attttgtaga tttgcaggat gtatcatctc
    41881 ttttggaaag agatacagtc cttcttgttg aagagtacgg agtgactcgt tataagaaat
    41941 ttgagcaaag tcttcaaatt ctcagaaact taaatcgttc tatcctcggt gtagcaacct
    42001 ttaaattata agtattctga tgtataaggt cttcaaaatc ggctcttagc cgattttgaa
    42061 ggctttttct atctgtctgc agaaattttc tcttggagtc tcctatagaa agaccctagg
    42121 gaaaatctat ccgtatagag gagccttctt ttgataaaat cgtaaaaatc tagtataata
    42181 gatagaactg aaagtatgag gttactagca atgaaaatga aacaaattag tgatacaact
    42241 ttaaaaatca cgatgtcttt agaggatttg atggatcgtg gaatggagat tgctgacttt
    42301 ctcgttcctc aagaaaaaac agaagagttc ttttatgcta tcttggatga gctagagatg
    42361 cctgatagct ttctggatac aggcatgttg agcttccgtg tgactccaaa acctgataag
    42421 gtagatgtct ttgtgaccaa gtcaaagatt gaccaaaatc tagattttga agacttatcg
    42481 gatttgccag atatggaaga attggctcaa atgtctccag atgaatttat caaaaccctg
    42541 gaaaaaagca tcgcagacaa aaccaaggat gatatcgaag ccattcaatc tcttgagcaa
    42601 gttgaagcca aggaagaaga gcaagagcag gctgaacaag aagctgagag taagaaagaa
    42661 ccttacatct actacatcct ttcttttgct aagttggctg acttggtagc ttttgccaag
    42721 acagtgactt ttgaaatgga aacttctgaa ctctacaaaa tgaacgagcg ctattatttg
    42781 accattttag tggatattga aaatcatcca agcccatatc cagcttggct gttggcccgt
    42841 atgcgcgagt ttgcagacga tagtgatatc agtcgctcag tcttacaaga gtatggtcaa
    42901 gtcttgatga gtcacgatgc agtgctcaat ctgcaaaaaa tcggctaatc ctttctggaa
    42961 agctattttc agattttaag aagagcgaat cgtctgattc gttttttctt tttatactga
    43021 aatagtgatt tactataata ggaattttca caaaattctg ttataatggc tatattagaa
    43081 aatttcgagg agataaagat gacagttaaa attgctttac taggatttgg taccgttgca
    43141 agtggtgtgc ctttcctcct aaaggaaaat ggaggaaaaa tcaatcaatc agcacattca
    43201 gatatcaaag ttgctaaggt attggtcaag gatgaagatg aaaaaaatcg cttgcttgca
    43261 gcagggaatg actttaactt tgtaaccaat gtggatgata ttttatcaga ccaggatatt
    43321 actatcgtag tggaattgat ggggcgtatt gagcctgcta aaacctttat cactcgtgcc
    43381 ttggaagctg gaaaacacgt tgttactgct aacaaggacc ttttagctgt ccatggcgca
    43441 gaattgctag aaatcgctca agctaacaag gtagcacttt actacgaagc agcagttgct
    43501 ggtgggattc caattcttcg tactttagca aattccttgg cttctgataa aattacgcgc
    43561 gtgcttggag tagtcaacgg aacttccaac ttcatggtga ccaagatggt ggaagaaggc
    43621 tggtcttacg atgatgctct tgcggaagca caacgtctag gatttgcaga aagcgatccg
    43681 acgaatgacg tagatgggat tgatgcagcc tacaagatgg ttattttgag ccaatttgcc
    43741 tttggcatga agattgcctt tgatgatgta gcccacaagg gaatccgcaa tatcacacca
    43801 gaagacgtag ctgtagctca agagcttggt tacgtagtga aattggttgg ttctattgag
    43861 gaaacttctt caggtattgc tgcagaagtg actccaacct tcctacctaa agcgcaccca
    43921 cttgctagtg tgaatggcgt aatgaacgct gtctttgtag aatctatcgg tattggtgag
    43981 tctatgtact acggaccagg tgctggtcaa aaaccaactg caacaagtgt tgtagctgat
    44041 attgtccgta tcgttcgtcg tttgaatgat ggtactattg gcaaagactt caacgaatat
    44101 agccgtgact tggtcttggc aaatcctgaa gatgtcaaag caaactacta tttctcaatc
    44161 ttggctctag actcaaaagg tcaggtcttg aagttggctg aaatcttcaa tgctcaagat
    44221 atttccttta agcaaatcct tcaagatggc aaagagggtg acaaggcgcg tgtcgttatc
    44281 atcacacaca agattaataa agcccagctt gaaaatgtct cagctgaatt gaagaaggtt
    44341 tcagaattcg acctcttgaa taccttcaag gtgctaggag aataagatga agattattgt
    44401 acctgcaacc agtgccaata tcgggccagg ttttgactcg gtcggtgtag ctgtaaccaa
    44461 gtatcttcaa attgaggtct gcgaagaacg agatgagtgg ctgattgaac accagattgg
    44521 caaatggatt ccacatgatg agcgtaatct cttgctcaaa atcgctttgc aaattgtacc
    44581 agacttgcaa ccaagacgct tgaaaatgac cagtgatgtc cctttggcgc gcggtttggg
    44641 ttcttccagc tcggttatcg ttgctgggat tgaactagcc aaccaactgg gtcaactcaa
    44701 cttatcagac catgaaaaat tgcagttagc gaccaagatt gaagggcatc ctgacaatgt
    44761 ggctccagcc atttatggta atctcgttat tgcaagttct gttgaagggc aagtctctgc
    44821 tatcgtagca gactttccag agtgtgattt tctagcttac attccaaact atgaattacg
    44881 tactcgcgac agccgtagtg tcttgcctaa aaaattgtct tataaggaag ctgttgctgc
    44941 aagttctatc gccaatgtag cggttgctgc cttgttggca ggagacatgg tgaccgctgg
    45001 gcaagcaatc gagggagacc tcttccatga gcgctatcgt caggacttgg taagagaatt
    45061 tgcgatgatt aagcaagtga ccaaagaaaa tggggcctat gcaacctacc tttctggtgc
    45121 tgggccgaca gttatggttc tggcttctca tgacaagatg ccaacaatta aggcagaatt
    45181 ggaaaagcaa cctttcaaag gaaaactgca tgacttgaga gttgataccc aaggtgtccg
    45241 tgtagaagca aaataaagaa tagaagatag gatggggaaa ctcttgacca gagggtttca
    45301 tatccttttt gtgaaaagaa gtttatactc aatgaaaatc aaagagcaaa ctaggaagct
    45361 agccgcaggc tgctcaaaac agtgttttga ggttgtggat agaactgacg aagtcagctc
    45421 aagacactgt tttgaggttg cagatagaac tgacgaagtc agtaacatac ctacggtaag
    45481 gtgacgctga cgtggtttga agagattttc gaagagtatt agttaaaaac gtgataaagg
    45541 agaaataaaa atggcagaaa tttatctagc aggtggttgt ttttggggcc tagaggaata
    45601 tttttcacgc atttctggag tgctagaaac cagtgttggc tacgctaatg gtcaagtcga
    45661 aacgaccaat taccagttgc tcaaggaaac agaccatgca gaaacggtcc aagtgattta
    45721 cgatgagaag gaagtgtcac tcagagagat tttactttat tatttccgag ttatcgatcc
    45781 tctatctatc aatcaacaag ggaatgaccg tggtcgccaa tatcgaactg ggatttatta
    45841 tcaggatgaa gcagatttgc cagctatcta cacagtggtg caggagcagg aacgcatgct
    45901 gggtcgaaag attgcagtag aagtggagca attacgccac tacattctgg ctgaagacta
    45961 ccaccaagac tatctcagga agaatccttc aggttactgt catatcgatg tgaccgatgc
    46021 tgataagcca ttgattgatg cagcaaacta tgaaaagcct agtcaagagg tgttgaaggc
    46081 cagtctatct gaagagtctt atcgtgtcac acaagaagct gctacagagg ctccatttac
    46141 caatgcctat gaccaaacct ttgaagaggg gatttatgta gatattacga caggtgagcc
    46201 actctttttt gccaaggata agtttgcttc aggttgtggt tggccaagtt ttagccgtcc
    46261 gatttccaaa gagttgattc attattacaa ggatctgagc catggaatgg agcgaattga
    46321 agttcgttct cgttcaggca gtgctcactt gggtcatgtt ttcacagatg gaccgcggga
    46381 gttaggcggc ctccgttact gtatcaattc tgcttcttta cgctttgtgg ccaaggatga
    46441 gatggaaaaa gcaggatatg gctatctatt gccttactta aacaaataaa acagagagtg
    46501 gggcttccca ctttcttcat ttctagaata tgaatagaag ggatttatga aatacctatt
    46561 atcttacttc aaaccctaca tcaaggaatc aattttagcc cccttgttca agctgttaga
    46621 agctgttttt gagctcttgg ttcccatggt gattgctggg attgttgacc aatctttacc
    46681 tcagggagat caaggtcatc tctggatgca gattggcctg ctccttatct ttgcagtaat
    46741 tggcgtttta gtggccttga tagctcaatt ttactcagca aaggcagcag taggctttgc
    46801 taaggaattg acaaacgatc tttatcgtca tattctttcc ttgcccaagg acagcagaga
    46861 ccgtctgaca acttctagtt tggtcactcg cttgacttcg gatacctacc agattcagac
    46921 tggtatcaat caattcctgc gtctcttttt gcgagcgccc attatcgttt ttggtgccat
    46981 ttttatggct tatcgaatct cagctgagtt gactttctgg ttcttagtca tggttgccat
    47041 tttgaccatt gtcattgtag ggttatctcg attggtcaat cctctctaca gtagtctcag
    47101 aaagaaaacg gaccaactgg ttcaggaaac gcgccagcaa ttgcaaggga tgcgggttat
    47161 tcgtgctttt ggtcaagaaa aacgagagtt acagattttt caaaccctta accaagttta
    47221 tgctagatta caagaaaaga caggtttctg gtctagttta ttaacacctc tgacctatct
    47281 gattgtcaat ggaactcttc tcgttattat ctggcaaggc tatatttcaa ttcaaggagg
    47341 agtgctcagt caaggtgctc tcattgctct tatcaattac cttttacaga ttttggtgga
    47401 attggtcaag ctagccatgt tgatcaattc cctcaaccag tcctatatct cagccaagcg
    47461 aatcgaggaa gtctttgttg aggctccaga ggatatccat tcagagttag aacaaaagca
    47521 agctaccaga gataaggttt tacaagtcca agaattgacc tttacctatc ctgatgcagc
    47581 ccagccttct ctgagagaca tttcctttga tatgactcaa ggacaaattc taggtatcat
    47641 cgggggaact ggttctggta aatcaagctt gattcaactc atacttggac tttatccagt
    47701 aaacaaggga agaattgacc tttatctaga tggattgagt cctcttaatt tggagcaatg
    47761 gagatcttgg attgcctatg tgccacaaaa ggtcgaactc tttaagggaa ctattcgttc
    47821 caacttgact ttaggtttaa atcaagaagt gactgatcaa gaactctggc aagccttgga
    47881 aattgctcaa gctaaggatt ttgtcagtga taaggaagga ctcttggatg ctctagttga
    47941 agcaggaggt cgaaatttct caggtggaca aaaacaaagg ctgtctatcg cacgagcagt
    48001 cttgcgccag gctccgtttc tcatcctaga tgatgcgacc tcggcactgg ataccattac
    48061 cgagtccaag ctcttgaaag ctattagaga aaattttcca aacatgagct taattttgat
    48121 ctctcaacga acctcgactt tacagatggc ggaccagatt ctcctcttgg aaaaaggtga
    48181 gttgctagct gttggcaagc acgatgactt gatgaaatcc agtcaagtct atcgtgaaat
    48241 caatgcatcc caacatggaa aggaggacta gaatgaaacg acaaactgta aaccagacgc
    48301 tcaaacgttt agtcgtagat ttagcaaacc atcccttcct ccttttccta gcctttctag
    48361 gaactattgc ccaagttggc ttatcaattt acctacctat tctgattggg caggtcattg
    48421 accaagtcct agtggctggt tcatcaccag ttttttggca gatttttctc cagatgctct
    48481 tggtggtaat aggaaatact ctggtacaat gggccaatcc tctcctctat aatcgtctaa
    48541 tcttctctta taccagagat ttacgagagc gaatcatcca taaactccat cgtttaccga
    48601 ttgcctttgt agataggcaa ggcaatggag agatggtcag tcgtgtaacc acggatatcg
    48661 aacagttggc agctggcttg accatgattt ttaaccaatt tttcattggt gttttgatga
    48721 ttttggttag tattctagcc atgctccaaa ttcatctcct catgactctc ttagtcttgc
    48781 tgttgacgcc actgtccatg gtgatttcac gctttattgc caagagatcc tatcatctct
    48841 tccagaagca aacagagacg aggggaattc agactcagtt gattgaagaa tcgcttagcc
    48901 agcagactat aatccagtcc ttcaatgctc aaacagaatt tatccaaaga ttgcgtgagg
    48961 ctcatgacaa ctactcaggc tattctcagt cagccatctt ttattcttca acggtcaatc
    49021 cttcgactcg ctttgtaaat gcactcattt atgccctttt agctggagta ggagcttatc
    49081 gtatcatgat gggttcaacc ttgactgtcg gtcgtttagt gacttttttg aactatgttc
    49141 agcaatacac caagcccttt aatgatattt cttcagtgct agctgagttg caaagtgctc
    49201 tggcttgcgt agagcgtatc tatggagtct tagatagccc tgaagtggct gaaacaggta
    49261 aggaagtctt gaccagtgac caagttaagg gagctatttc ctttaaacat gtctcttttg
    49321 gctaccatcc tgaaaaaatt ttgattaagg acttgtctat cgatattcca gctggtagta
    49381 agatagccat cgttggtccg acaggtgctg gaaaatcaac tcttatcaat ctccttatgc
    49441 gtttttatcc cattagctcg ggagatatct tgctggatgg gcaatccatt tatgattata
    49501 cacgagcatc attgagacag cagtttggta tggtgcttca agaaacctgg ctcacacaag
    49561 ggaccattca tgataatatt gcctttggca atcctgaagc cagtcgagag caagtaattg
    49621 ctgctgccaa agcagctaat gcagactttt tcatccaaca gtttccacag ggatacgata
    49681 ccaagttgga aaatgctgga gaatctctct ctgtcggcca agctcagctc ttgaccatag
    49741 cccgagtctt tctggctatt ccaaagattc ttatcttaga cgaggcaact tcttccattg
    49801 atacacggac agaagtgctg gtacaggatg cctttgcaaa actcatgaag ggccgcacaa
    49861 gcttcatcat tgctcaccgt ttgtcaacca ttcaggatgc ggatttaatt cttgtcttag
    49921 tagatggtga tattgttgaa tatggtaacc atcaagaact catggataga aagggtaagt
    49981 attaccaaat gcaaaaagct gcggctttta gttctgaata agccattctc ttttgaaagt
    50041 ttatggacga aaaaagttgc cttcgagtga cttttttgtt acaatagcta gaaaaattgt
    50101 tcactgtaat actcaatgaa aatcaaagag caaactagga agctagccgt aggttgctca
    50161 aagcacagct ttgaggttgt agataagact gacgaagtca gctcaaaaca ctgttttgag
    50221 gttgcagata gaactgacga agtcagctca aaacactgtt ttgaggttgc agatagaact
    50281 gacgaagtca gctcaaaaca ctgttttgag gttgcagata gaactgacga agtcagtaac
    50341 cacacctacg gcaaagtgaa tctgaagtgg tttgaagaga gtacaacttg tcttttaaaa
    50401 aaggagtcta taatgaaagt ctttcagcat gtaaatatcg tgacttgtga tcaaaatttc
    50461 catgtttatc ttgatggaat cttagcagtc aaggattctc aaatcgtcta tgttggtcaa
    50521 gataagccag catttttaga acaagctgag cagattatag actatcaggg agcttggatt
    50581 atgcctggtt tggtcaattg tcacacccat tctgcaatga caggtctgag agggatccga
    50641 gatgacagca atctccatga atggctcaat gactatatct ggccagcaga atctgagttt
    50701 actcccgaca tgactaccaa tgcggtcaaa gaagccctga cagagatgct ccagtcagga
    50761 acaacaacct ttaacgatat gtataatccc aatggtgtgg atatccagca aatttatcag
    50821 gtggtgaaaa cttccaagat gcgttgttat ttttctccga ctctcttttc ttcagagaca
    50881 gaaacaactg ctgagactat aagcagaact cgatccatca tagacgaaat cttaaaatat
    50941 aaaaatccaa atttcaaggt tatggtagca cctcattctc cgtatagctg cagtagagac
    51001 ttgctggaag cgagtttgga aatggcaaaa gagctaaata ttccgctcca tgtccatgtg
    51061 gcggagacca aggaggagtc aggaattatc ctcaaacgat acggcaaacg cccccttgct
    51121 tttcttgaag aactgggtta tttagatcat ccgtccgtat ttgctcacgg ggtcgaatta
    51181 aacgagagag aaattgaacg cttggcatct tctcaagtgg ctatcgccca caatcctatc
    51241 agtaacctca aactggcatc aggaattgct ccaattatcc agctccaaaa agcgggagta
    51301 gcagtcggaa ttgcgactga ctcggttgct tccaataaca atctagatat gtttgaggaa
    51361 ggaaggactg cagctcttct tcagaagatg aaaagtgggg atgccagcca gtttccaatc
    51421 gaaacagctc tcaaggtact gacaatcgaa ggggctaagg cccttggaat ggaaaatcag
    51481 ataggaagtc tggaagtcgg caagcaagca gattttctgg tcattcaacc acaagggaaa
    51541 attcatctcc aacctcagga aaatatgctg tctcacctgg tttatgcagt taaatctagt
    51601 gatgtagatg atgtttatat cgccggagaa caggttgtta agcaaggtca agtcctgaca
    51661 gtagaacttt aaaagaaaaa tcacgaaaaa ttttaaaaaa agtttgcaaa aatcttgcat
    51721 tctttttttg actatgctat acttatatac ggtttaaaaa aactgcctaa gacagtaggg
    51781 gagctcgact cataaatatc ctaccgagga caagacgtat catgtaaaaa gaagcgtatt
    51841 gtactttcgt gtctaggttt gggcgcgttt ttctttttga aaaattcccc aagcaaaata
    51901 attacggagg tgaacacact aatgagtgaa gcaattattg ctaaaaaagc ggaactagtt
    51961 gacgtagtag ctgaaaaaat gaaagctgct gcatctatcg tcgttgtaga cgctcgtggt
    52021 ttgacagttg agcaagatac agttcttcgt cgtgagcttc gtggaagcga agttgagtat
    52081 aaagttatta aaaactcaat cttgcgtcgt gcagctgaaa aagctggtct tgaagatctt
    52141 gcatctgtat ttgttggacc atctgcagta gcattttcta atgaagatgt tatcgcacca
    52201 gcgaaaatct tgaacaactt ttctaaaaac gctgaagcac ttgaaattaa aggtggtgca
    52261 atcgaaggcg ctgtcgcatc taaagaagag attcttgcac ttgcaactct tccaaaccgc
    52321 gaaggacttc tttctatgct cctttctgta cttcaagcgc cagtgcgcaa cgttgctctt
    52381 gcagtcaaag cggttgcaga aagcaaagaa gacgcggctt aatcttaagc tacacagcgt
    52441 agcctagcta cgaaaaaaac tattataaaa tttaaaactt atttggagga aataacaatg
    52501 gcattgaaca ttgaaaacat tattgctgaa attaaagaag cttcaatcct tgaattgaac
    52561 gaccttgtaa aagctatcga agaagaattt ggtgtaactg cagctgctcc tgtagctgtt
    52621 gctgcagctg atgcagctga tgctggtgct gctaaagatt cattcgacgt tgaattgaca
    52681 tctgcaggcg acaaaaaagt tggcgttatc aaagttgtac gtgaaattac tggtcttggt
    52741 cttaaagaag ctaaagaact tgttgacggt gcaccagcac ttgttaaaga aggcgttgca
    52801 actgcagaag ctgaagaaat caaagctaaa ttggaagaag ctggagcttc agttactctt
    52861 aaataagagg catataccaa ttagaattcg gaatactata gtatcagcct tttagaatcg
    52921 tgaacacatt ttagaaactg ataaatttat ttagtttcct accaaaaacc ctaccaaaaa
    52981 ttctttggca gggtttttct ttttaataaa ttttcggagg tagacactag attgtctacc
    53041 cctttttttg tgtttcggag gtaattatgc aaaaggaagg acagtcatca cgtcaattcg
    53101 tgaagagtca tctcatgact atcatgggag ttgatattgt aaaagcaact cagttagttg
    53161 atgaaatgga acaagtaggt ttaatccgat ttgatgaatt tggaaatgtt ggaattttag
    53221 tcttggaggg acaatcatga agcgtattac cgctagtcaa tatcagacat cagagcgtta
    53281 ttataagctc cccaaacttt tatttgagag tgaacggtat aagaatatga aactggaggt
    53341 taaggtggtt tactcagttt taaaagatcg gttggagttg tctttgagca aagcttggat
    53401 tgatgaggat ggggctattt atttgattta ttccaattca aatttgatgg cacttttagg
    53461 ctgttcaaag tcaaaattac tctccatgtg agtttgaggt gacattttta gatgattacc
    53521 ataaaaaaca taattaccca ctattttacg aatcctatct tcaaaacgtt atggaattcc
    53581 ttgaaagtca agacataaag aatggggttg atgcctttgt agatgatcat caaaatctcg
    53641 tttttgtttt atatggacaa ggctatcgag ccgagggaaa agagggaata cttacaaccc
    53701 aagtaactgt aaaagcttat gatgaagaca agaaaccgat taacttcgca aatttattag
    53761 attccttaat cgtctcagaa tatcaaatgg aaccgaatct ttgggaggtc tcccatgatt
    53821 gatctctatc taagtaaaaa tagacaaaga aatcaacttc ttttagattt cttccaaaac
    53881 tatggcatag aggtatcttg tcattcagtt tctgaaatga caaaggacaa attaattgag
    53941 atgatgagct attcttcaga ttgttttgag tttttatctc ccaatttatt acgatttaag
    54001 aaccgtgaca atctaagatt aacggatttc gttgagataa ttttaaaaaa tcctgaacta
    54061 accatcagac ttcctcttgc ggtttcaaat aagcgagttt atccaagtct gaatttggaa
    54121 gaggctagag ctctattgcc aagagatacg aaacaattga tttacatggc gcaaacacat
    54181 tatttatcta actaaaggag aagcaatatg gctgaacgat tttgggagaa cttgtccatt
    54241 attttggctg aaagaaatat cagctgtatt gagttaacca gaaaaatgtt tgcgggagag
    54301 tttcactatc caagtgaatt gaatcgtttg tatcaaaaga ttcgccacta gaagatggaa
    54361 caacgaatgc ctcaaagttc atgggtagaa aggattgtgc ag
//
LOCUS       DAVKHZ010000005        53647 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000005 DAVKHZ010000000
VERSION     DAVKHZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.005"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(105..1466)
                     /gene="dnaA"
                     /locus_tag="VWW99_000241"
     CDS             complement(105..1466)
                     /gene="dnaA"
                     /locus_tag="VWW99_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9538143.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(1679..2437)
                     /locus_tag="VWW99_000242"
     CDS             complement(1679..2437)
                     /locus_tag="VWW99_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9538144.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(2495..3676)
                     /locus_tag="VWW99_000243"
     CDS             complement(2495..3676)
                     /locus_tag="VWW99_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9538145.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            3859..4338
                     /gene="rlmH"
                     /locus_tag="VWW99_000244"
     CDS             3859..4338
                     /gene="rlmH"
                     /locus_tag="VWW99_000244"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9538146.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4382..4455
                     /locus_tag="VWW99_000245"
     tRNA            4382..4455
                     /locus_tag="VWW99_000245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4416..4418,aa:Arg,seq:tct)
     gene            4630..5103
                     /gene="tnpA"
                     /locus_tag="VWW99_000246"
     CDS             4630..5103
                     /gene="tnpA"
                     /locus_tag="VWW99_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEU9538147.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            5359..5484
                     /gene="comC"
                     /locus_tag="VWW99_000247"
     CDS             5359..5484
                     /gene="comC"
                     /locus_tag="VWW99_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9538148.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            5505..6830
                     /locus_tag="VWW99_000248"
     CDS             5505..6830
                     /locus_tag="VWW99_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9538149.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            6827..7579
                     /locus_tag="VWW99_000249"
     CDS             6827..7579
                     /locus_tag="VWW99_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538150.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            7631..7702
                     /locus_tag="VWW99_000250"
     tRNA            7631..7702
                     /locus_tag="VWW99_000250"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7664..7666,aa:Glu,seq:ttc)
     gene            7708..7781
                     /locus_tag="VWW99_000251"
     tRNA            7708..7781
                     /locus_tag="VWW99_000251"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7742..7744,aa:Asn,seq:gtt)
     gene            complement(7822..8364)
                     /locus_tag="VWW99_000252"
     CDS             complement(7822..8364)
                     /locus_tag="VWW99_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9538151.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            8522..9289
                     /locus_tag="VWW99_000253"
     CDS             8522..9289
                     /locus_tag="VWW99_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU9538152.1"
                     /translation="MIGISLIPDLYNIIFLSLMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            9374..9649
                     /locus_tag="VWW99_000254"
     CDS             9374..9649
                     /locus_tag="VWW99_000254"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538153.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFLQQYQQI"
     gene            9739..10053
                     /locus_tag="VWW99_000255"
     CDS             9739..10053
                     /locus_tag="VWW99_000255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9538154.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(10154..12706)
                     /locus_tag="VWW99_000256"
     CDS             complement(10154..12706)
                     /locus_tag="VWW99_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9538155.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(12768..14390)
                     /locus_tag="VWW99_000257"
     CDS             complement(12768..14390)
                     /locus_tag="VWW99_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9538156.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            14596..15621
                     /gene="trpS"
                     /locus_tag="VWW99_000258"
     CDS             14596..15621
                     /gene="trpS"
                     /locus_tag="VWW99_000258"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9538157.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            15773..17251
                     /gene="guaB"
                     /locus_tag="VWW99_000259"
     CDS             15773..17251
                     /gene="guaB"
                     /locus_tag="VWW99_000259"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9538158.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(17302..18399)
                     /gene="recF"
                     /locus_tag="VWW99_000260"
     CDS             complement(17302..18399)
                     /gene="recF"
                     /locus_tag="VWW99_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9538159.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(18402..18770)
                     /gene="yaaA"
                     /locus_tag="VWW99_000261"
     CDS             complement(18402..18770)
                     /gene="yaaA"
                     /locus_tag="VWW99_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9538160.1"
                     /translation="MEYKLFEEFITLQALFKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQASRFPGR"
     gene            18929..20179
                     /locus_tag="VWW99_000262"
     CDS             18929..20179
                     /locus_tag="VWW99_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9538161.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            20176..21459
                     /locus_tag="VWW99_000263"
     CDS             20176..21459
                     /locus_tag="VWW99_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9538162.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            21501..22322
                     /locus_tag="VWW99_000264"
     CDS             21501..22322
                     /locus_tag="VWW99_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9538163.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            22333..22878
                     /gene="pgsA"
                     /locus_tag="VWW99_000265"
     CDS             22333..22878
                     /gene="pgsA"
                     /locus_tag="VWW99_000265"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9538164.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            22875..23702
                     /locus_tag="VWW99_000266"
     CDS             22875..23702
                     /locus_tag="VWW99_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9538165.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            23687..24526
                     /locus_tag="VWW99_000267"
     CDS             23687..24526
                     /locus_tag="VWW99_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9538166.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQFLNGLLVPSQGSVRIFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            24519..25313
                     /locus_tag="VWW99_000268"
     CDS             24519..25313
                     /locus_tag="VWW99_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9538167.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            25372..26190
                     /gene="mreC"
                     /locus_tag="VWW99_000269"
     CDS             25372..26190
                     /gene="mreC"
                     /locus_tag="VWW99_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9538168.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRTKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            26190..26684
                     /gene="mreD"
                     /locus_tag="VWW99_000270"
     CDS             26190..26684
                     /gene="mreD"
                     /locus_tag="VWW99_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9538169.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            26778..27956
                     /locus_tag="VWW99_000271"
     CDS             26778..27956
                     /locus_tag="VWW99_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9538170.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            28180..28959
                     /gene="rpsB"
                     /locus_tag="VWW99_000272"
     CDS             28180..28959
                     /gene="rpsB"
                     /locus_tag="VWW99_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9538171.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            29038..30078
                     /locus_tag="VWW99_000273"
     CDS             29038..30078
                     /locus_tag="VWW99_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9538172.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            30324..30647
                     /gene="tnpB"
                     /locus_tag="VWW99_000274"
     CDS             30324..30647
                     /gene="tnpB"
                     /locus_tag="VWW99_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9538173.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(30788..31075)
                     /locus_tag="VWW99_000275"
                     /pseudo
     CDS             complement(30788..31075)
                     /locus_tag="VWW99_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            31066..31464
                     /locus_tag="VWW99_000276"
     CDS             31066..31464
                     /locus_tag="VWW99_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538174.1"
                     /translation="MKAPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(31661..32581)
                     /gene="cysK"
                     /locus_tag="VWW99_000277"
     CDS             complement(31661..32581)
                     /gene="cysK"
                     /locus_tag="VWW99_000277"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9538175.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(32866..33501)
                     /locus_tag="VWW99_000278"
     CDS             complement(32866..33501)
                     /locus_tag="VWW99_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9538176.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            33557..34855
                     /locus_tag="VWW99_000279"
     CDS             33557..34855
                     /locus_tag="VWW99_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9538177.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            34852..35514
                     /locus_tag="VWW99_000280"
     CDS             34852..35514
                     /locus_tag="VWW99_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9538178.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            35594..36142
                     /gene="raiA"
                     /locus_tag="VWW99_000281"
     CDS             35594..36142
                     /gene="raiA"
                     /locus_tag="VWW99_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9538179.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            36278..38251
                     /locus_tag="VWW99_000282"
     CDS             36278..38251
                     /locus_tag="VWW99_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9538180.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            38248..38700
                     /gene="rplI"
                     /locus_tag="VWW99_000283"
     CDS             38248..38700
                     /gene="rplI"
                     /locus_tag="VWW99_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9538181.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            38744..40096
                     /gene="dnaB"
                     /locus_tag="VWW99_000284"
     CDS             38744..40096
                     /gene="dnaB"
                     /locus_tag="VWW99_000284"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9538182.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            40098..40364
                     /locus_tag="VWW99_000285"
     CDS             40098..40364
                     /locus_tag="VWW99_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538183.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            40453..41799
                     /gene="cbpD"
                     /locus_tag="VWW99_000286"
     CDS             40453..41799
                     /gene="cbpD"
                     /locus_tag="VWW99_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9538184.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      41882..41984
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            42055..42345
                     /locus_tag="VWW99_000287"
     CDS             42055..42345
                     /locus_tag="VWW99_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9538185.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            42308..43066
                     /locus_tag="VWW99_000288"
     CDS             42308..43066
                     /locus_tag="VWW99_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538186.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            43105..44112
                     /locus_tag="VWW99_000289"
     CDS             43105..44112
                     /locus_tag="VWW99_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9538187.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            44112..44840
                     /locus_tag="VWW99_000290"
     CDS             44112..44840
                     /locus_tag="VWW99_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538188.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            44959..45417
                     /locus_tag="VWW99_000291"
     CDS             44959..45417
                     /locus_tag="VWW99_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9538189.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            45419..47851
                     /locus_tag="VWW99_000292"
     CDS             45419..47851
                     /locus_tag="VWW99_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9538190.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            48493..49146
                     /gene="rr06"
                     /locus_tag="VWW99_000293"
     CDS             48493..49146
                     /gene="rr06"
                     /locus_tag="VWW99_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9538191.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            49143..50483
                     /gene="hk06"
                     /locus_tag="VWW99_000294"
     CDS             49143..50483
                     /gene="hk06"
                     /locus_tag="VWW99_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9538192.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            50566..51093
                     /locus_tag="VWW99_000295"
     CDS             50566..51093
                     /locus_tag="VWW99_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9538193.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDSMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            51172..53166
                     /gene="pspC"
                     /locus_tag="VWW99_000296"
     CDS             51172..53166
                     /gene="pspC"
                     /locus_tag="VWW99_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9538194.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTETVALINELQGIKKTYLY
                     NLNVLKEKSKVELPSKIKAKLDVAFDQFKKDTLKPGEKVAEAQKKVAEAKKKAEDQKE
                     EDRRNYPTNTYKTLELDIAESDVKVKEAELELVNEEAKPRNEEKIKKAKAKVESEKAE
                     AIRLEEIKTDREEAKRKADAKLKEAVEKNAANSEQGEPKRRVKRGVLGEPATPDKKEN
                     DAKSSDSSVGEETLPSPSLKPEKKVAEAEKKVEEAKKKAEDQKEEDRRNYPTNTYKTL
                     ELEIAESDVKVKEAELELVNEEAKPRNEEKIKKAKAKVESEKAEATRLEKIKTDRKKA
                     EEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAEEPNPAPAPKPEKPADQPKAEKPA
                     DQQAEEDYARRSEEEYNRLTQQQPPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGW
                     LQNNGSWYYLNSNGAMATGWLQYNGSWYYLNANGDMATGWFQYNGSWYYLNANGDMAT
                     GWLQYNGSWYYLNSNGAMVTGWLQNNGSWYYLNANGSMATDWVKDGDTWYYLEASGAM
                     KASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(53370..>53647)
                     /locus_tag="VWW99_000297"
     CDS             complement(53370..>53647)
                     /locus_tag="VWW99_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538195.1"
                     /translation="PEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHYSNA
                     KLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT        16977 a         8752 c        11298 g        16620 t
ORIGIN      
        1 tgtgaaatct gtggaaaaca gcctttaaac aagtcatatc aagtttttta gcttgtttaa
       61 aaaatgtgga taaaaaagat aaaaaactaa aagttatcca caaattattt gattttcttt
      121 ttgattgatt caatttctaa acgtaaatta tcgtcttgat caatcaaaga ttttattttg
      181 gcatgggcat gaatgactgt ggtatgatct tttcccccaa attccttccc aatttttgga
      241 agactattat ctgttagttc tctagataaa tacatggcta cttgacgggc caaaacaata
      301 ttttgaaggc gtctacttcc cttcatttct ttgatactaa caccataaaa gttaccaact
      361 tcagtttgga ttttatcaat tgggatgacg agcatttggc taacatcttg tttgcgggct
      421 ctaatggctt ctgcagcaat atcaatagtg atatccttga ttttttttac tctggcaatt
      481 aaagtgatgt cgttgatggc tccctcaaga tctcgaacat ttgaatcaaa ttgcccagct
      541 aggtattcta gagtatcact ttggaaattg tagcctaaat gttccgtctt actttgtaaa
      601 atggcaatac gtgtttcaaa gtcagggggt gtgatagttt gtgtcaatcc ccaactaaaa
      661 cgcgtgacaa gcctctcctc gagcccttct agatgttttg gactacgatc actcgttagg
      721 acaatctgtt tttgcttgtc atgaagggcg ttaaaggtat tgaaaaattc ttcctgagtt
      781 gcgacttttt ttccgctgag tgactgtata tcatcgatta acaaaagatc aagactacga
      841 taggtctttt taaacttttc catttcccca agtcttaggt gatcaagaaa gtcattaata
      901 aagctttcgg cagggatata tttaacacgc gcattaggaa tattttttag aatttcattt
      961 ccaatagcgt ttaataagtg agtcttacca aggcctggtc ctccatagat aaaaagaggg
     1021 ttataggtca gagccaaatc ttcagagaca gctaaagcgg ctgatacagc ccaaacattt
     1081 ccatcccctt gaataaagtt atcaaaggta tacttttctt ttaatcccgt atctgaataa
     1141 ggaatagata ctaactttgg actatagtca taaagagtta aatttgtagc ttcttcaact
     1201 tgtgagctag tcgtatcttg aggtttggtg aaaatatagt ggggagttat ttcagcgtca
     1261 taaatttcaa aaccagctac tacaataata tcttttagtt gtttttccca gaccatttcc
     1321 atttcagagc gaggtagaaa tatagtggca acattttcct ctaccttgat gagttcagct
     1381 tgaatagcat agaaatcata catggatcga gtcagtcttt cttgtgcaaa ttctaatata
     1441 cgattccaaa attgtttttc tttcaatcct ttctcctcct ttactattta aaagtttaat
     1501 actagagata ttttaccata gatagcaaga attttccaca agctgtgaaa aataatccac
     1561 aatgttacca aactttatcc acaggttggg gataaaagaa gaaattattg atttattaag
     1621 ctttttatcg aaaaacatag tggataacct tgtgagataa aaaaataaaa gaacagcctt
     1681 atttcaggct gttgataatt ctactatatt cttcttgatt tgaaaaagaa ataatgattt
     1741 ttccactgtc ttttttagat agtttaattt ctacatctaa tccgagtagt tttcttaact
     1801 gtttttcttc attttgtatg aaatgattag ttttttgctg tttcttttgt tttttctctg
     1861 tcagaagagc ttctaatttc cttacagaaa tatcttcttc tataatccgt tgaaagaaat
     1921 agtcttgttg ttccttattt aacccaacta gggaacgcgc atgggcttgt gatagtttgc
     1981 cattttctac ttctgaaaga atctgttctg gcaaggaaag taaacgaatg gagttgctga
     2041 tatatggacg agacttgccc atcttatctg caatttcagc atgggtgaat cctttctcta
     2101 cgagagattc ataggcgcgt gcttcttcta ttgggtttaa attttctctc tgtaaatttt
     2161 caataatgga ctggaccatc atctcttggt ctgaaatctg tttaacaaca gctgggatag
     2221 accgtagacc agctaaaagt gaagcccgat agcgtctctc tcctgcaagg atttcataac
     2281 caataacagg agattgacga acaataatcg gttgaatgac cccattttct ttgatagact
     2341 gtgctagttc atctagtttt tctctatcaa attcttttcg gggttgatag ggattttttt
     2401 gtatatctgt gatagaaatc atttcaaatt tttccatgat tctacactaa cacatctttt
     2461 ctcttatgta aagctttctt tacatagatg tcaatcaaga ttctaaatca cctgaactct
     2521 tgttaagttt gatagaggta gtttcttctt tcccgttacg atagtaggtt atcttaatgg
     2581 tgtctccgat agaatggttg taaagagcac tttgtaagtc tgttgatgaa gcaatctctt
     2641 tgtcatctac ttttgtaatt acatcgtatt tttcaaggtg accattggca ggcatattac
     2701 tttgtaccga acgaacaact acaccagatg taacattact tggaatattg agtcttctga
     2761 tgtcgcttgt actcacatta gataaattaa ccatctggat tcccaaagct ggacgcgtca
     2821 cttttccgtt tttttctaac tgttcaataa tattgatagc atcatttgca ggaattgcga
     2881 aaccaagacc ttctacagat gttcctccat ttgtagcaat tttacttgag gtaattccga
     2941 taacctgccc ttgaatattg atcagtgggc cgccagagtt acctgggtta atagcagtat
     3001 cagtttggat ggcttttgta gaaatagctt gtccatcttc cgatcttaag gatacatttc
     3061 tattgagact ggatacgata ccttgagtga cagtatttgc atattcagaa cctaacgggc
     3121 taccgatggc aatagcagtt tctcctacag ttaacttact agaatcacca aactcagcta
     3181 ctgttgtcac tttttctgaa gagattttga cgacagcaat atcagagaaa gtgtcagctc
     3241 cgacaatttc tccaggtact ttagtcccat ctgacaatct aatatctact ttgctggcgc
     3301 catttataac gtgattgttg gtgacgatgt aagcttcttt atcattcttt ttataaataa
     3361 ctccagatcc ttcactagag attcgctgag aatctgtgtc agtatcatca ttgccaaata
     3421 cgctattttg tctgtttgcc gaataagtaa taacagaaac aacagcatct tttactttgt
     3481 taacagcctg tgttgttgaa ttttcgttct tataggcagt ttgtgtaata gtactattgt
     3541 tgttagagtt gtttacacta cttttttgag ttagttgagt tattgaaaaa ctacccaagg
     3601 ctccactaaa aaagctaatg acgataacga ctaataattg aaaccatttt ttgtaaaatg
     3661 tttttagatg tttcatattt gcctccatat gtttgaatta ctgaaagtat aaactgacta
     3721 gcttaattat aacttaaaca caaaagtttt acacaaactg tggataactc ttttgaaact
     3781 gtgattttct taattgaaat ctatttttta ttttgtgaat aagatgtgaa aaaatagaga
     3841 atatgttaga atagagtcat gaaaattaaa gttgtaacag ttgggaaact gaaagaaaag
     3901 tatttaaaag atggtatcgc agagtattca aaacgaattt ctagatttgc taagtttgaa
     3961 atgattgagt tatcagatga aaaaacacca gataaggcca gtgaatcaga aaatcaaaag
     4021 attttagaaa tagaaggtca gagaatttta tcaaaaattg ctgaccgtga tttcgttatt
     4081 gtgttagcca ttgaagggaa aactttcttc tcagaagaat ttagtaagca gttagaagaa
     4141 acttctataa aaggattttc tactcttact tttattattg ggggaagttt aggattgtca
     4201 tcatctgtaa aaaatagagc caatctttct gtcagttttg gtcgcctaac cttaccccat
     4261 cagttaatga gactagttct tgttgaacaa atctatcgcg cttttacgat tcagcaggga
     4321 ttcccctacc ataaatagaa aattgacttt taattgaatt tttggtagaa taattgtatt
     4381 aggtctcata gctcagctgg atagagcatt cgccttctaa gcgaacggtc gcaggttcga
     4441 atcctgctgg gatcaatata atagcaaagc tgggaatttt cccggctttt ttcttgttgt
     4501 ctcccgaaaa ccactagcta tgctagtggt tccatagagc ttttagcgtg gtaacaaaaa
     4561 gaacctccta aaatgataag atagaagtgg tttctccgcc actacaacat atcatacagg
     4621 aggtacctca tgagagagga taatcaaagt ttatcacata ccacatggaa ttgtaaatat
     4681 catattgttt ttgcacccaa atatcgtcgt caaatcattt atggcagata caaagctagt
     4741 atcggaagaa tcatacgtga cttatgtgag cgtaagggtg taataatcca tgaagcgaat
     4801 gcttgttcag accatattca catgcttatc agtattcctc cgaaacttag tgtttcgtcc
     4861 tttatgggct atttaaaggg caagagcagt ttgatgattt ttgataagca tgcgaattta
     4921 aaatacaaat atggcaatcg caagttttgg tgtagaggct attatgtaga tacggtaggc
     4981 cgtaatcaga aagtgatagc tgaatatatt cagaatcaat tacaagaaga cagagtagca
     5041 gaccagctca cgttattcga gtcagtagat ccgtttactg gcgaaataaa taagaggaag
     5101 taactaaggt gctttagcac ctgctcggga aagtggtgcg cgaggaagct atttcggtgg
     5161 gcctttggcc ctggccggta gaagcggctt atagccgcag aacaaaccac cagttcacac
     5221 tggtggtttt gattaaaaaa gtacactttg ggagaaaaaa atgacagttg agagaatttt
     5281 atctaaaacg aaattccatt ttgtataatg gtttttgtaa gttagcttac aagaaaaaac
     5341 attttaggag attttattat gaaaaacaca gttaaattgg aacagtttgt agctttgaag
     5401 gaaaaagact tacaaaagat taaaggtggg gagatgaggt tgtcaaaatt cttccgtgat
     5461 tttattttac aaagaaaaaa gtaatgaaat aaggggaaag agtaatggat ttatttggat
     5521 ttgggacggt tattgttcat tttttaatta ttagtcacag ttaccatttt atttgtaaag
     5581 gtcaaataaa tagaaaagaa ttattcgttt ttggtgctta tacattacta acagaaatag
     5641 tatttgattt tcccttatat attctatatt tagatgggtt agggattgaa agatttttat
     5701 ttcctttggg attatattcc tattttcgat ggatgaaaca gtatgagagg gatagaggac
     5761 tattcctaag tttactacta tctcttttat atgagagcac tcataacttt ctgtccgtaa
     5821 ctttctcctc tataacagga gataattttg ttttacaata tcatttccca ttctttttcg
     5881 ttgtgacggt gttaacctat tttgttacat taaaaatcat ttactatttc catttggaac
     5941 tagcctattt tgacgaggac tacctttatc ctttcttgaa aaaagtattt tttgctttac
     6001 tattgctaca tattgtatct ttcgtttcag atatggtaag tacgattaaa catttgaata
     6061 gttttggaag tattttgtca tctattgtct ttatctctct ccttttgacc ttctttgcaa
     6121 tgaattctca taaagttcaa atggagaaag agattgcttt gaagcagaag aaatttgaac
     6181 agaaacattt acagaattac acagatgaaa ttgttggtct gtataatgaa atccgtggtt
     6241 ttcgacatga ttatgctgga atgcttgtca gcatgcagat ggcaattgac agtggtaatt
     6301 tacaggaaat tgacagaatt tacaatgaag ttttagtcaa agcaaatcat aaattgcgtt
     6361 cagataagta cacttacttt gatttgaaca acatagaaga ctcagcttta cgaagtttgg
     6421 ttgctcagtc aattgtctat gctcgaaata atggtgtaga gtttacactg gaagtaaaag
     6481 atacgattac caagcttcca attgaactat tggatttggt tcgtatcatg agcgttttat
     6541 tgaataatgc tgtcgaagga tcggctgata gctataaaaa gcagatggaa gtagcagtta
     6601 ttaagatgga aactgaaaca gttattgtga ttcagaattc atgtaaaatg acgatgactc
     6661 cttcaggaga tctatttgcc ttaggattct ccactaaggg aagaaatcgc ggagtcggat
     6721 taaataatgt gaaagaacta ctagataagt acaacaatat tattttagaa acagagatgg
     6781 aaggcagtac atttagacaa atcattagat ttaagaggga atttgaatga aagttttaat
     6841 tttagaagat gttattgaac atcaagtgag actagagaga atattggatg aaatttcgaa
     6901 agaatcgaat attccaatat catacaagac aacgggaaaa gtccgtgaat ttgaagaata
     6961 cattgaaaat gatgaagtaa atcagcttta tttcctagat atcgatattc atggaattga
     7021 gaaaaaggga tttgaagtgg ctcagctcat tcgtcattac aatccttacg ctattatcgt
     7081 ctttatcact agtcgatcag agtttgcgac tctaacctat aaatatcagg tatcagccct
     7141 agattttgtt gataaggata tcaatgatga gatgtttaag aagagaattg agcaaaatat
     7201 cttctacacg aagagtatgt tacttgaaaa tgaagatgtt gtagattatt tcgactacaa
     7261 ttacaaggga aatgatttaa aaattcctta ccatgatatt ttgtatattg aaacaacagg
     7321 ggtatctcat aaattgcgca ttattggtaa gaattttgca aaagagtttt atggtaccat
     7381 gacagatatt caggaaaagg acaaacatac tcagcgattt tattctcctc acaagtcatt
     7441 tttggtaaat ataggcaata tcagagaaat tgatcgaaaa aacttagaaa ttgttttcta
     7501 tgaagaccat cgttgtccta tttcaagatt aaaaattaga aaattaaaag atattttaga
     7561 gaaaaaatct caaaagtgat tgacaattag caagaaattg atataatggt tatatctgct
     7621 acagatagaa ggtccgttgg tcaaggggtt aagacaccgc cttttcacgg cggtaacacg
     7681 ggttcgaatc ccgtacggac tattttatcc gccatagctc agttggtagt agcgcatgac
     7741 tgttaatcat gatgtcgtag gttcgagtcc tactggcgga gtatagataa aagggaacac
     7801 agctgtgttc ctctttttgt atcaatttgt atcaccaagc attttcataa ggaagtctgt
     7861 tatttcttga gaactttctt tttttccatg tgcaatccaa gtttggcaga caccaaaaag
     7921 tgcatgagtt agatagatgc tactatattc taattcggtg gtatttagat tcagttgcat
     7981 aaatcgcttt tgtaaatctg tactaagcat gatatgaagt ttatttcgta agaaattttg
     8041 gatttcttta gtcccatttt cagaaagaag ggcagccaga agtggttctg actctagata
     8101 ttcaaaaact tctaaaatag cgtctctttt gtgatgagca tgtttttgaa aaatatattc
     8161 aaatgtatgg aatagcttgc tttgatagtg ctcaatcata tcatacttat ccttatagtg
     8221 agtatagaag ctggaacgac taattccggc tttttctact aatttgacag tagaaatttt
     8281 atcaaatggc tgttccatca gtaattgtac catagcattt tcaatagttc gctttgtttt
     8341 taagcgtttg ttactttctt gcatatttcc tccttgtaaa caaattagac tatatgtcta
     8401 aaaatagatt ttttaatgta taatctatta tatcaaaatt ttagacaata tgtttaaaaa
     8461 aggagaaact aagtttaaag aatggaaagc aatttaaaaa aaaccaacct ttattattgt
     8521 catgatcggg atttctctta ttccagatct gtacaatatc atatttttgt cattaatgtg
     8581 ggatccatat gggcaattgt ctgacttacc tgtggcagtt gtaaataatg ataaagaggc
     8641 ttcctataat ggtaatacta tggcaatagg aaaagacatg gtgtccaatt taaaagaaaa
     8701 taaaaccttg gattttcatt ttgtagatga agaggaagga aagaagggat tggaagatgg
     8761 cgattactat atggtagtga ctttaccaag tgatttatct gaaaaaacaa ctacattatc
     8821 caatattcaa tcgacagcag cttatcaatc attgacaagt gagcaacaaa ctgagataag
     8881 tgattctgta tctcaaaatt caactgatag tattcaatcg gctcagtcaa ttgtagcttt
     8941 agtacaagat ttacagggaa gtttagaaaa cttacaaaat caatcttcta atctttcgac
     9001 tttaaaaaat caatctaatc aagtatcacc tattacttct acttctttga taggattgtc
     9061 aagtggatta acagagatac aaggagatgt tactagcaaa ttagttcctg ccagtcagtc
     9121 gattgcatca ggtgtaaacg catatactac aggtgttgat aaagtttctc agggcgcaag
     9181 tcaactaagt gaaaaaaatg ccaccttgac aggtagtttg gataaactag tttcaggctc
     9241 aaacaccttg acacaaaaat cttctagatt gacagcagga gttggttaat tacaatcagg
     9301 atctgggcaa ttagcagaca aatccagtca gttactttca ggtgcttctc cattagagaa
     9361 tagagctaat aaattggcag atggatctgg gaaactagca gaaggtggaa caaagttaac
     9421 ttctggattg gaagatttac agacaggact tgcttcttta ggacaaggac taggtaatgc
     9481 tagtgatcaa ctcaaatcag tatcaacaga atctaaaaat gcagagattt tgtcaaatcc
     9541 actcaatctt tcaaaaacag acaatgatca agttcctgta aatggaatcg caatagctcc
     9601 ttatatgata tcagttgctc tttttttgca gcaatatcaa caaatatgat atttgcgaaa
     9661 ttgccttcag gacgtcatcc agagagccgt tgggcttggt tgaaatcttg agctgaaata
     9721 aatggtatta tagctgtttt ggcaggaatt ttggtatatg gaggagttca gcttattggt
     9781 ttaactgcta atcatgagat gagaatattt attctcatca tcctaacaag tttagtattc
     9841 atgtctatgg tgaccacttt agcaacgtgg aatagccgta taggagcttt tttctcactt
     9901 attttgcttt tactacagtt agcatcaagt gcaggtactt atccacttgc tttgacaaat
     9961 gatttcttta gatctattaa tccctggtta ccaatgagct attcagtttc gggattacga
    10021 caaacaatct ctatcaacaa gtcattttcc tagctgtcat actagttcta tttactagtt
    10081 taggtatgct agcctatcaa cataagaaaa tggaagaaga ttaaaaaaat cgaccgatta
    10141 actggtcgat tttttatgcc ttagatgact ttcgtctgtg attatagatt ccaaatagta
    10201 agagagaagt aaaggaacag attgctccag taataaaacc attgggaatg aaggaaagtg
    10261 taatagttcc tttccccttg ggaatgtcaa ctttcataaa tccagtttga gcttgtttaa
    10321 tttctatttt cttaccatct tggtaggcag accaaccttt gtcataagga atggtgaaga
    10381 aaatagatgt atcttgttgg acatcatatg tagcaaaaac cttgttttta gaagttgata
    10441 ctgtgacagg ttgttcttta attttttgaa ttgcctcggt gaaagttttg gtatctaaac
    10501 gatagaaggt aggagattca aatgatactt gtgaatttcc agggaaacta acattgatat
    10561 tgaaagtttt tttctcttta gtatatccta gattaaagaa ggagaagaca ttatcagttg
    10621 taaaagtctt tttttcacca tttacaagga tgtcaacctt cttttgttta tcgttagaaa
    10681 agtgaaggtt tatgaaagag agataaactt ggctgttttc tggaacttca atttgatact
    10741 ggattgctgc atcttcattt gaagaacttg tgacactaat caaatcatta gtattttcta
    10801 ttttttctgt tttttcataa ggtattggag aaaaataatc aaaattgacg ttagcaagtt
    10861 gatttaaaaa tgaggcctga ttatccaagg tatgttcatt gaacttgaca tcattgtaaa
    10921 cagattgact cgcaactgca atcggaagag agtattgatt ttcatatagg gtaagattat
    10981 ctttttgata gatatcttta aagccatact tatcaatagg actgtctgag atattgtact
    11041 ggataccaaa taaactatca gccaaaatac tattatttgc atatcggaga ttgagattag
    11101 tcccagagga tttaaaacca agtttatcta aagtagagct tgatgaacga tttcgaacag
    11161 atgaaaattg agagattcca ttgtagttga atttcatact gtcatttcct gtctgagttt
    11221 gtagtttttc agtacgagta aattgatttc caatatatgt tgagaaagat tccatagctg
    11281 ggatatctcg actataagca cttcgagaag caaatcccca ttccttagca attccgtcca
    11341 tttgagatga agcatttaaa ctcatttcaa ccagtataaa taaagagatt agaatggcaa
    11401 atagattcac agatataaac tttttgataa ctgcaaggag taaaagagaa tagacaacca
    11461 aaaattcaag agtaagcaga atattcaaat ctgttaaaaa agaataatgc gattttagat
    11521 agatggtagc taaaaatcct gctactacaa gaaaaagcga aactaaaaaa ttccagactt
    11581 taagttcttt cagacgcttt aagacttctg ctgctgtgta aattaacaag gtagagaaaa
    11641 tccaagcata gcgatgtaaa aacatgtttg gagtatgcat gccttgccaa aataagtcaa
    11701 gagcttctat gtaaaagctt gcaattagaa atgcaaagaa tattacatat atgagtttca
    11761 cgtgaaactt aatagatttc agcgtaaaaa ataaaatggt caaaataaag ggaaatagtc
    11821 caacaaaaat cattgggatg gccccatact ttgttgtgtc aaaggaacca atgaattgct
    11881 tagcaaagag atcaagatac cagctacttt cagtttgaaa ctttgtaact tcagtcaatt
    11941 tttccccatg tgtctgtaaa tcaaatagag tgggaagagt cataatcaaa ctagccatac
    12001 cagctaaaaa ggagataact atgaaatcaa gaacagatga ttttcgagtc ttaaagtccc
    12061 acgaaatttg acagagatac cagaaaataa gaaacaatac tgtcatatat ccaaaataat
    12121 aattttgaat aaataagatt gacagacttg taaagtacaa taggagtttc ttttcagtta
    12181 tcagtagatg taaaccagtt ataattaaag gaatcaagat aaaaacatct agccaggttt
    12241 ttatctctaa ttgactgaca gtgaaactca tcagagcata ggaagtagat aaggctagtt
    12301 ttaaaatctg agggatagat tgaaacaatt tattcaaact aaaaaaggtt gacagaccaa
    12361 tcaatccaaa ttttaagaga gttgtcagat agatagcatc tggcatattc gttagatcaa
    12421 aaaagtaaac cagaggcgcg agaaaactac ccaagtaata actagatagg gcatagaagt
    12481 ttagccctag accacttgta aaggtgtaaa acagattact atttccatgt aggatatttc
    12541 gtaaggctac atcaaaaata acgtattgat gaaagccatc tcctaataga ggagagttgt
    12601 cgctattcca gtagatacct tgagatagat atactccaga cataatcact acaggaatga
    12661 tgaaagaaat aaaataggtt cgatatgttt ttaaaaatga tttcatgtta cctcgtagaa
    12721 tgatagaaaa ctcagttggt taacccaact gagttttgaa gttttattta gtctttccaa
    12781 agttctttaa cttttgcttg tacttctgca ttttctagga attcatcgta ggtttcatcg
    12841 atacggtcaa tgacgccatt tttagataag acaatgatat ggttagccaa agtttgaata
    12901 aattcgtggt catggctggc aaagatgatt gattctttaa agtttttcaa tccatcattc
    12961 aagcttgaga tagattccaa gtccaagtga tttgttggat catcaagtac aaggacattt
    13021 gattttaaga gcatgagttt tgaaagcatg acacgaactt tttctccccc tgacaagaca
    13081 tttacaggtt tgttaacttc atctccagag aagagcatac ggccgaggaa gccacgtagg
    13141 aaagtattgt catcttcttc tttacttgcg aattgacgca accagtcaag aattgattct
    13201 cctcctgcaa aatcagctga gttatctttt ggtaggtaag attgactagt tgtaactccc
    13261 cacttgacag ttccttcata gtcaatatct cccatgattg cacgaattaa tgcagtcgtt
    13321 tgaatatcat tttgtccaat aagtgctgtc ttatcatctg gacgcaagat gaaactaata
    13381 ttatccaaga tagtttcacc atcaatcttt acagttaaat tttctactgt caagagatca
    13441 ttaccaatct cacgttccgc tttaaagttg ataaatggat atttacgact agatggcaca
    13501 atctcttcta gctcaatctt atcaagcatt ttcttacgtg atgttgcctg ccttgactta
    13561 gaagcattgg cagagaaacg agcaacaaat tcttgcaatt gtttaatttt ttcttctgct
    13621 ttagcattac ggtctgctag caatttagca gcaagctcag aagattcctt ccagaagtcg
    13681 tagtttccga catagagttt gatttttcca aagtcaaggt cggccatgtg agtacaaact
    13741 ttgtttaaga agtgacggtc gtgggatact acgataactg tgttatcaaa gtcaatcaag
    13801 aagtcttcta accaagtaat cgattggata tccaaaccgt tagtaggctc gtccaagaga
    13861 agaacatctg gtttaccaaa aagtgctttg gcgaggagaa cctttacttt ttcaccgttg
    13921 gccaattcgc tcatgttttg gtagtgtaat tcttctggaa tgtttaggtt ttgaagtagt
    13981 tgagaggctt cactctctgc ttcccaacct ccaagttcgg caaactctcc ttcgagttcg
    14041 gcagcacgaa ccccgtcctc gtctgagaaa tcttccttca tgtagatagc atctttctct
    14101 ttcatgatgc tataaagttt ttcatttccc atgataacga catcaatggc acgttcatct
    14161 tcgtagtcaa agtgattttg acgaagaaca gagagacgtt catctggacc aagagagatg
    14221 tgaccagtag taggttcgat atctccagct aaaattttta aaaaggttga ttttccggca
    14281 ccattagcac cgattaatcc gtaagtattt ccttctgtaa atttgatatt gacatcatca
    14341 aaaagtttgc gatcactaaa acgtagtgaa acatcagata ctgtaagcaa tgtttttctc
    14401 ctatatgtgt aatatattta ttctactaga aaatacagaa atattcaaat ttttatttgt
    14461 caattttgtg taaattatat ttacagtatc ctttacacaa atctgtaaaa agcaaggctg
    14521 atttattttg ataaattacg gttatttcat taaaaaaatg ctataattga aaggactata
    14581 tcgaaggaga acaaaatgac taaacccatt attttaacag gagaccgtcc aacaggaaaa
    14641 ttgcatattg gacattatgt tggaagtctc aaaaatcgag tattattaca ggaagaggat
    14701 aagtatgata tgtttgtgtt cttggctgac caacaagcct tgacagatca tgccaaagat
    14761 cctcaaacca ttgtagagtc tatcggaaat gtggctttgg attatcttgc agttggattg
    14821 gatccaaata agtcaactat ttttattcaa agccagattc cagagttggc tgagttgtct
    14881 atgtattata tgaatctagt ttcgttagca cgtttggagc gaaatccaac agtcaagaca
    14941 gagatttctc agaaaggatt tggagaaagc attccgacag gattcttggt ctatccaatc
    15001 gctcaagcag ctgatatcac agctttcaag gctaattatg ttcctgttgg gacagatcag
    15061 aaaccaatga ttgagcaaac tcgtgaaatt gttcgttctt ttaacaatgc atataactgt
    15121 gatgtcttgg tagagccgga aggtatttat ccagaaaatg agagagcagg gcgtttgcct
    15181 ggtttagatg gaaatgctaa aatgtctaaa tcactaaata atggtattta tttagctgat
    15241 gatgcggata ctttgcgtaa aaaagtaatg agtatgtata cagatccaga tcatatccgc
    15301 gttgaggatc caggtaagat tgagggaaat atggttttcc attatctaga tgtttttggt
    15361 cgtccagaag atgctcaaga aattgctgat atgaaagaac gttatcaacg aggtggtctt
    15421 ggtgatgtga agaccaagcg ttatctactt gaaatattag aacgtgaact gggtcctatt
    15481 cgtgagcgcc gtattgaatt tgctaaggat atgggagaag tttataatat gattcaaaaa
    15541 ggtagtgaaa gagcgcgtga agttgcgggt caaaccctat ctgaggtaaa aggggcaatg
    15601 ggacttcatt actttaacta agtttatttt acaagaaact atcttttcta tctcgaagaa
    15661 aagatagttt tttatttacc cctgtaacat ttgacaaatt tttatagaat ggtatgatag
    15721 atacaatata aaaagagtca agctcaaaaa gaaagaaaag aggaaacttc gaatgtctaa
    15781 ttgggacact aaatttttga aaaaaggttt tacctttgat gatgtattgc ttattccagc
    15841 tgaaagtcat gtgttgccta acgatgcaga tttaacaact aaattggcag ataatttgac
    15901 tttaaatatc ccaattatta ccgctgccat ggacacagtt acagagagtc aaatggccat
    15961 tgctattgct cgtgcaggcg gtctcggagt tatccataaa aacatgtcaa ttgctcaaca
    16021 agcagacgag gttcgtaagg taaaacgttc tgaaaatgga gttattattg atccgttctt
    16081 cttgacgcct gaacatacaa ttgctgaagc agatgagctt atgggtcgtt accgcatcag
    16141 tggtgttcca gttgttgaaa cacttgaaaa tcgtaaattg gttggtattt tgacaaaccg
    16201 agatcttcgt tttatttcag attataatca accaatttca aaccatatga ctagtgaaaa
    16261 tcttgttact gctcctgtgg gtacggatct tgcaacggct gagagtattc ttcaagagca
    16321 tcgtattgaa aaacttccgt tggtcgatga agaaggcagt ctttctggtt tgatcactat
    16381 caaagatatt gaaaaagtta ttgagtttcc aaatgcagct aaagatgagt ttggtcgtct
    16441 tctagttgca ggtgcagtag gtgttacttc agatacattt gaacgtgcag aggctctttt
    16501 tgaggcagga gcggatgcga ttgttattga tactgcacat ggtcattctg caggtgtctt
    16561 gcgtaaaatt gccgagattc gtgctcattt cccagatcgg actttgattg ctggaaatat
    16621 tgctactgct gaaggtgcac gtgcccttta tgaagcgggt gtagacgttg ttaaggttgg
    16681 tattggacca ggttctatct gtactactcg tgtgattgct ggtgttggtg ttccgcaagt
    16741 aacagctatc tacgatgctg cagctgttgc gcgcgaatat ggtaaaacga ttattgctga
    16801 cggtgggatc aagtattctg gagatattgt aaaagcactt gctgcaggtg gaaatgctgt
    16861 tatgcttgga tctatgtttg ctggaactga tgaagctcca ggcgaaactg aaatcttcca
    16921 aggacgtaaa ttcaagactt accgtggtat gggatcaatt gctgctatga agaaaggttc
    16981 aagcgaccgt tatttccaag gttctgtcaa tgaagcaaac aagcttgttc cagaaggaat
    17041 tgaaggtcgt gttgcttata aaggagcggc agctgatatt gttttccaaa tgattggtgg
    17101 tattcgctct ggtatgggtt actgtggtgc agctaacctt aaagaactac acgataatgc
    17161 tcaatttatt gaaatgtctg gtgctggttt gaaagaaagc catcctcatg atgtgcaaat
    17221 tactaatgag gcaccaaatt attctatgta aaaaacaatg aaaagaactc cagtgaaaac
    17281 aggagttctt ttacaatgtt gtcaatttcc atttacagca gctttaccat cctgaatagt
    17341 gaagatactt agattttctg gcagattttg aagatggtct aagcttgttg ttgtgataaa
    17401 ggtttggatt gattgagaaa tcgtttctaa taattttaac tgtctagtgt tgtcaagttc
    17461 actcatcaca tcgtcaagca gtaatatagg agattctgtg gtaatgcttt ccattaattc
    17521 gatttctgct aattttatcg agaggacgag actacgatgt tgaccttggc ttccgaaact
    17581 agcatccatc ccatttatat aaaaagaaat gtcatctcga tgaggaccga caccagtatt
    17641 ctttttaaat aaatctctgg atctactttt ttctaaagca attttgaaag attcggataa
    17701 gttttgtttg tcagttatat tgacagaaga ttgataggat attgacaact cttcgatctg
    17761 attagagagt tcaaaatgtt tcttacgccc aaatgattct agttttttta tgaaatctaa
    17821 gcggtgattc attacacgac atccataatc aactagctga tcatctaaca cagaaaggaa
    17881 tgtttcatct attttttgag ctgattttag gtaagtgttt ctttgcttta ggatgtggtt
    17941 ataattggtt aagtcagata aatagattgg cttaatttgc ccaagttcca tatcaatgaa
    18001 ttttcgtcga atcgaaggtg ctcctttaat tagttgtaaa tcttcaggag caaataagac
    18061 aacattcatg tgtcctacat aatctgaaag gcgtgcctgt tttaagtgat taacttttgt
    18121 tacacgacct ttttgtgtta gttcgatttc taggggaatg gatccagttt ttttctgaac
    18181 gagacctgaa agatgaagtt gttcctcatc aaaatgaatg agatttttat ctgttcgagt
    18241 tcgatgacta cgcgttaagg ctaaaaaata gatagcctct aacatgtttg tttttccttg
    18301 tgcattacgt cccaaaaaga cattcaattt aggattaaag tctattttcg tttctttgta
    18361 attacgaaaa gtcttgagag atagatgttg tagccacatg attatctacc agggaatcgt
    18421 gaagcttgtt ggcttttagg tgatgaagca ggtttcgatt tgtctttttt aactccctta
    18481 ttcatctttt tgacaagttt agcgatccgt tctttttcaa ctttatcagc ttggtattca
    18541 tcttgttctt cagaagtagg ttgtgtcaac aagatgtcaa tgttcatgtc agggatgtca
    18601 actttatcgc caatacgaag tttttttcca cgacggctct ctaattccct attaaagtaa
    18661 acagaatgtt cagagagaaa tgatttaata gctcctccgc tatgtgtaat tccaagttct
    18721 ttgaatagtg cttggagggt aataaattct tcaaataatt tgtattccat aattcacctc
    18781 aatctttggt attataccat attttcctaa aaatagtgag agagacagcg agaaatgtaa
    18841 gcgattttta tttcaaaagt gttacaggga acaagaaaat ttcaggtttt cgtgatataa
    18901 tagaagtctg tatataagga ggtaaatcat ggagttagtg catggaattt caacacattt
    18961 tatccaatca aaaaagttta aaacaaacaa aattaccgtg cgttttaccg ctccattatc
    19021 ccttgatacg attgcaggtc acatgttgag tgcaagtatg ctagagactg ctaatcagat
    19081 gtaccccact tctcaagatt tgaggagaca cttggccagt ctatacggta cagatatgtc
    19141 aaccaattgt ttcagaagag ggcaaagcca cattatagaa ttgacattta cctatgttcg
    19201 tgatgagttt ttaagtagga aaaacgtgct aacctctcag attttggaac ttgtaaaaga
    19261 aactcttttt tcacccgtag tagttgataa tgggtttgat ccggccttat ttgaaattga
    19321 gaaaaaacaa ttgctagcaa gtttagcagc tgatatggat gattcttttt attttgcaca
    19381 taaagaattg gataaattgt tttttcatga tgaacgtctt caattggaat atagtgattt
    19441 acgaaatcgt attttagctg aaactccaca aagttcttat tcttgtttcc aagaattttt
    19501 agccaatgat cgaatagatt tctttttcct aggtgatttt aatgaggttg aaattcaaaa
    19561 tgtattagaa tcatttggct ttaaaggtcg aaaaggagat gtgaaggttc agtattgtca
    19621 accttattct aatatccttc aggaaggtat ggttcggaaa aatgtgggac aatccatttt
    19681 ggaattaggt tatcattact gttctaaata tggtgatgag caacatttac ccatgattgt
    19741 aatgaatggt ttacttggtg gatttgctca ctctaagctc tttacaaatg tccgtgaaaa
    19801 tgctggatta gcttatacca tttcaagtga gcttgattta tttagtggat tcttgaggat
    19861 gtatgctggt atcaatcgag aaaatcgtaa ccaggctcgt aaaatgatga ataatcaact
    19921 gcttgattta aaaaaaggtt attttacaga gtttgagtta aatcagacca aggaaatgat
    19981 tcgttggtcg ttgttacttt ctcaagataa tcaatcttca ttgattgaac gtgcttatca
    20041 aaatgcctta tttggaaaat cttcagcaga ctttaaaagt tggattgcaa agcttgaaca
    20101 aattgacaaa gatgctattt gtagagtagc taataatgtg aaactacaag cgatttactt
    20161 tatggaagga atagaatgac aaaggttgtt tttgaagaaa aatactatcc agctgtaaaa
    20221 gaaatggttt atcgaactcg tttggccaac ggattgacag ttgctctttt gcctaaaaag
    20281 gaatttaaag aggtttacgg gagtgtcact gtacagtttg gttcggtaga tacgtttgtc
    20341 acagaagttg acggagatgt aaaacaatat cctggaggaa ttgctcattt tcttgaacat
    20401 aaattatttg agagagaaga ttctagtgat ttgatgtcgg cttttacgag tctaggtgca
    20461 gatagtaatg cctttacaag ctttacaaaa acaaactatc ttttttcagc aacggattat
    20521 tttttagaaa atttagattt acttgatgaa ttggtaacat cagcacactt tactgaagct
    20581 tccattctga cagagcagga tattattcag caagaacgag aaatgtacca agatgatcca
    20641 gattcgtgtt tattcttttc aactttagcg aatttgtatc ctggtacacc tttagcaact
    20701 gatatagttg gaagtgagga gtccatttcc caaatcaatc taactaattt gcaagaaaat
    20761 tttacaaagt tttacaaacc tgtaaacatg tctctgtttt tagttggtaa ttttgatgtg
    20821 gagcgagtac aggactattt tgaaagcaaa gaactgaaag attcagattt tcaggaagta
    20881 gcaagagaaa agttgttttt acagcctgta aagccaacag atagtatgag aatggaagta
    20941 tcttctccca aactagcgat tggagttaga ggtaagcgag aagtttctga agcggattgc
    21001 tatcgacatc atattttatt aaaattattg tttgcaatga tgtttggttg gacttcggat
    21061 cgttttcaaa aatgttatga atcaggtaaa attgatgcgt ccttatctct ggaagttgaa
    21121 ataacaagtc gctttcattt tgtcatgttg acaatggata cgaaagagcc agttgctttg
    21181 tctcatcaat ttaggaaggc tattcgtaat tttacaaagg atttagatat tacagaggaa
    21241 catttagata ttatcaaaag agagatgttt ggcgaatttt tcagtagcat gaactctctt
    21301 gaatttattg caacgcaata tgatgctttt gaaaatggtg agacaatttt tgatttgccg
    21361 aaaattttac aggaaattac tttagaggat gtccttgatg ctggacatca tttaatagat
    21421 gatggtgaca tagttgattt tacaatattc ccatcgtagt aacctatcat aatagacact
    21481 agaaagaagg gatgacaagt atgagaaaaa aaacaattgg agaggtttta cgattagcta
    21541 gaatcaatca gggattgagt ttagatgaat tgcagaaaaa gacagaaatc cagttagata
    21601 tgttggaagc aatggaagca gacgatttcg atcaacttcc aagtcctttt tacacgcgtt
    21661 ctttcttgaa aaaatatgca tgggctgttg agttagatga ccaaattgtt ttggatgctt
    21721 atgattctgg gagtatgatt acttatgagg aagtagatgt tgatgaagat gagttgacag
    21781 gtcgtagacg ttcaagtaag aaaaagaaga aaaaaacatc atttttacct ttattttatt
    21841 ttatcctttt tgctttatcg attttaattt ttgtgactta ttatgtttgg aactatattc
    21901 aaactcaacc agaggagcct tctctttcta attacagtgt ggttcaatca acaagttcaa
    21961 ctagctctgt tccccactcc tcaagtagta gttcttctag tatagaatca gctataagtg
    22021 tatcaggcga aggaaatcat gtagaaatcg cttataagac aagtaaggaa acagttaaat
    22081 tgcaattggc agtttcagat gttacaagtt gggtcagtgt ttcagaaagc gaacttgagg
    22141 gcggtgtaac cttatcgcca aagaagaaaa gtgcagaagc aacagttgca actaaaagtc
    22201 ctgtaacaat tacgttaggt gttgtaaaag gtgttgattt gacagtagat aatcagactg
    22261 ttgatttatc gaaattaaca gctcagactg gacaaatcac tgtaaccttt actaaaaatt
    22321 aaggaaaaac gaatgaaaaa agaacaaatt cccaatctct taacaatagg tcgaattctc
    22381 tttataccta tttttatctt tattttaacg ataggaaatt cgatagagag tcatatagtt
    22441 gcagctatta tctttgctgt tgccagtatt accgactatt tagatggata tttagctcgt
    22501 aaatggaatg tggtcagtaa ttttggtaaa tttgcagatc ctatggcgga taagttacta
    22561 gttatgtcgg cttttattat gttgattgag ttaggtatgg ctccggcttg gattgttgca
    22621 gtgattatct gtcgtgagtt agctgtgaca ggtttaaggc ttttattggt tgaaactggt
    22681 ggaacaattt tagcagcagc aatgcctgga aaaattaaaa cttttagtca gatgtttgcc
    22741 attattttct tgctattaca ttggactttg cttggtcaag ttctacttta tgtagcctta
    22801 tttttcacta tctactctgg ctatgactat ttcaagggta gtgcctatgt atttaaaggg
    22861 acatttggtt cgaaatgaaa tcaataattg atgtaaaaaa tctttctttt cgctataaag
    22921 aaaatcagaa ctactacgat gtgaaggata ttacgtttca cgtgaaacgt ggagaatggc
    22981 tttcgattgt agggcataat ggtagtggta aatcaacgac ggttcgatta attgatggct
    23041 tactggaagc agaatccgga gagattgtaa ttgatggcca acgtttgact gaggaaaatg
    23101 tttggaatat acgtcgtcaa atcggtatgg tttttcaaaa tccagacaat caatttgttg
    23161 gagcgactgt tgaagatgat gttgcttttg gtttggaaaa tcagggactt tctcgtcaag
    23221 aaatgaaaaa gagagtagaa gaagctctgg ctttagttgg catgttggac tttaaaaaga
    23281 gagagccagc gcgtctatca ggtggccaaa agcaacgtgt ggccattgca ggtgttgtag
    23341 ccctaagacc agctatttta atcttagatg aagcaacgag tatgttggat cctgaggggc
    23401 gtagagaact tattgggaca gtaaaaggaa ttcgaaaaga ctatgatatg acggtcattt
    23461 ctattaccca tgatttggaa gaagtcgcca tgagtgatcg cgtattggtc atgaaaaaag
    23521 gggaaattga atcaactagt agtccaaggg agcttttctc tcgaaatgat ttagatcaaa
    23581 ttggattaga cgatcctttt gctaatcaat taaaaaaatc tttgagccag aatggctatg
    23641 atttacctga aaattatttg acagaaagtg agctagagga taagctatgg gaattgctct
    23701 agaaaatgtg aattttacat atcaagaagg tactccctta gcttcagcag ctttgtcgga
    23761 tgtttctttg acgattgaag atggctctta tacagcttta attgggcaca caggtagtgg
    23821 taaatcaact attttacaat tcttaaatgg tttattggtg ccaagtcaag ggagtgtgag
    23881 gatttttgat accttaatca cctcgacttc taaaaataaa gatattcgtc aaattagaaa
    23941 acaggttggc ttggtatttc agtttgctga aaatcagatt tttgaagaaa cggttttgaa
    24001 ggacgttgct tttggaccgc aaaattttgg agtttctgaa gaagatgctg tgaagactgc
    24061 gcgtgagaaa ctggctctgg ttggaattga tgaatcactt tttgatcgta gtccgtttga
    24121 gctgtcaggg ggacaaatga gacgtgttgc cattgcaggc atacttgcca tggagccatc
    24181 tatattagtc ttagatgagc caacagctgg tctagatcct ctagggagaa aagagttgat
    24241 gaccctgttc aaaaaactcc accagtcagg gatgaccatc gtcttggtaa cgcatttgat
    24301 ggatgatgtt gctgaatatg ctaatcaagt ctatgtaatg gaaaagggac gtttagtaaa
    24361 ggggggcaaa ccaagtgatg tctttcaaga cgttgttttt atggaagaag ttcagttggg
    24421 agtacctaaa attacggcct tttgtaaacg attggctgat agaggcgtgt catttaaacg
    24481 attaccgatt aagatagagg agttcaagga gtcgctaaat ggatagtatg attttggggc
    24541 gttatatccc aggggattcg attgttcacc gattggatcc acgtagcaaa ttgcttgcta
    24601 tgatgctact gattttgatt gtgttttggg ctaataaccc cttgacgaat ctgattcttt
    24661 ttatagcgac agggatattt attgccttgt caggagtatc tctttcattt tttattcagg
    24721 gcttgaagtc tatgtttttc ttgattgcct tcacaactat ttttcaacta tttttcattt
    24781 ctaatgggaa tgttttattt gagttttcgt ttgtgagaat cacggattat gctttgcaac
    24841 aagctgggat tattttttgt cgctttgtat tgattatttt cttttcaact ttgttaacct
    24901 taacaaccat gcctttaagt ttggcatcag ctgtcgaagc tttattagca cctttaaagc
    24961 gtgtgaaagt tccagttcat gaaattggat tgatgctgtc tatgagtttg cgttttgtcc
    25021 caaccttgat ggatgatacg acgcggatta tgaatgcaca gaaagctcgt ggagtggatt
    25081 ttggagaagg aagtatcgtt caaaaagtaa aggcgatgat tcccattttg attcctcttt
    25141 ttgcgacaag tttaaaacgt gcagattcct tggctatcgc tatggaagcg cgtggctatc
    25201 agggtggaaa aggcagaagt caataccgac aattgaaatg gactctaaag gatacgctga
    25261 ccattcttgt tattctcgta cttggttgtt gtttattttt cttaaaatct tagtagcttt
    25321 caggaattga taaaaagtta ctgtaacagt ttttgataga aaggtaggga tatgaaccgt
    25381 tttaaaaaat caaaatatgt cattattgtt tttgtcactg ttctgcttgt gtcagctctc
    25441 ttagcgacga cttattcaag tacaattgtg acaaaattag gagatggaat ctcattggtt
    25501 gatagagttg tacaaaaacc ttttcagtgg tttgattctg tcaaatcaga tttggctcat
    25561 ttgacacgaa catataatga aaatgaaagt ttgaagaaac agctttacca attagaagtt
    25621 aaatcaaatg aggtggaaag tttaaagaca gaaaatgaac aactgcgcca attgcttgat
    25681 atgaagtcta aattgcaagc cacaaagact ttagcagcag atgttattat gcgttctccg
    25741 gtatcttgga agcaggagtt gaccttagat gcaggtagaa caaaaggtgc ttctgagaac
    25801 atgttagcta ttgcaaatgg tggcttgatt gggagtgttt caaaagtaga ggagaactct
    25861 actatagtca accttctgac aaatacggaa aatgctgata agatttctgt taaaattcaa
    25921 catggctcta ctacaattta tggaattatt attggctatg acaaggaaaa tgacgttctt
    25981 aaaattagcc aattaaatag taatagcgat attagtgcgg gagataaggt gactactggt
    26041 ggattaggaa actttaacgt tgctgatatt cctgttggtg aagtggttgc cacaacgcat
    26101 agtacagact atttgacacg agaagtaact gttaaattga gtgcagatac tcataatgta
    26161 gatgtgatag aattagtggg gaattcataa tgagacagtt gaagcgagtt ggagtatttt
    26221 tattgcttcc tttctttgtt ctaattgacg cccatattag ccagcttctg ggctcatttt
    26281 tcccccatgt acatttggct agtcattttc tttttctatt tctcttattt gagacgatag
    26341 aagtatcaga gtatctctac ctagtctatt gttttgttat aggcttggtt tatgatgttt
    26401 actttttcca tctaataggg attacaactc tcttatttat cttattggga gccttccttc
    26461 ataaattgaa tagtgttatt ttgttgaatc gttggacaag aatgctagct atgattgtgc
    26521 tgacattcct gtttgaaatg ggtagttatc ttttggcttt tatggtaggg ttgacagtag
    26581 atagcatgtc gatttttata gtctatagct tggtaccgac gatgatttta aattttttat
    26641 ggattactgt ttttcaattt atttttgaaa aatattatct ataagaacga catataaatg
    26701 taacaaaggc gtaatattta ttaggccttt ttttggtata ctagtattgt ctttaaaaga
    26761 aggagtatct acgtaatatg aagaaaaaaa tcttagcgtc acttttatta agtacagtaa
    26821 tggtttctca agtagctgtt ttaacaactg cgcatgcaga aacgactgat gacaaaattg
    26881 ctgctcaaga taataaaatt agtaacttaa cagcacaaca acaagaagcc caaaaacaag
    26941 ttgaccaaat tcaggagcaa gtatcagcta ttcaagctga gcagtctaac ttgcaagctg
    27001 aaaatgatag attacaagca gaatctaaga aactcgaggg tgagattaca gaactttcta
    27061 aaaacattgt ttctcgtaac caatcgttgg aaaaacaagc tcgtagtgct caaacaaatg
    27121 gagccgtaac tagctatatc aataccattg taaactcaaa atcaattaca gaagctattt
    27181 cacgtgttgc tgcaatgagt gaaatcgtat ctgcaaacaa caaaatgtta gaacaacaaa
    27241 aggcagataa aaaagctatt tctgaaaaac aagtagcaaa taatgatgct atcaatactg
    27301 taattgctaa tcaacaaaaa ttggctgatg atgctcaagc attgactacg aaacaggcag
    27361 aactaaaagc tgctgaatta agtcttgctg ctgagaaagc gacagctgaa ggggaaaaag
    27421 caagtctatt agagcaaaaa gcagcagctg aggcagaggc tcgtgcagct gcggtagcag
    27481 aagcagctta taaagaaaaa cgagctagcc aacaacaatc agtacttgct tcagcaaaca
    27541 ctaacttaac agctcaagtg caagcagtat ctgaatctgc agcagcacct gtccgtgcaa
    27601 aagttcgtcc aacatacagt acaaacgctt caagttatcc aattggagaa tgtacatggg
    27661 gagtaaaaac attggcacct tgggctggag actactgggg taatggagca cagtgggcta
    27721 caagtgcagc agcagcaggt ttccgtacag gttcaacacc tcaagttgga gcaattgcat
    27781 gttggaatga tggtggatat ggtcacgtag cggttgttac agctgttgaa tcaacaacac
    27841 gtatccaagt atcagaatca aattatgcag gtaatcgtac aattggaaat caccgtggat
    27901 ggttcaatcc aacaacaact tctgaaggtt ttgttacata tatttatgca gattaattta
    27961 cagagggact cgaatagagc cctcttttca ggttttaccg tgacaatccc tattaaaaat
    28021 tatatcaaaa tagcttgaaa atattggaaa agtatggtaa aatgaaaatt gtcgtgtgaa
    28081 cgataatact cattcttgat gaattgtgaa gcagttgccc ttgggtcgtt ttgcgagttg
    28141 aagtcaagaa gaggaaaaaa acaaaaagga gaaatactca tggcagtaat ttcaatgaaa
    28201 caacttcttg aggctggtgt acactttggt caccaaactc gtcgctggaa tcctaagatg
    28261 gctaagtaca tctttactga acgtaacgga atccacgtta tcgacttgca acaaactgta
    28321 aaatacgctg accaagcata cgacttcatg cgtgatgcag cagctaacga tgcagttgta
    28381 ttgttcgttg gtactaagaa acaagcagct gatgcagttg ctgaagaagc agtacgttca
    28441 ggtcaatact tcatcaacca ccgttggttg ggtggaactc ttacaaactg gggaacaatc
    28501 caaaaacgta tcgctcgttt gaaagaaatt aaacgtatgg aagaagatgg aactttcgaa
    28561 gttcttccta agaaagaagt tgcacttctt aacaaacaac gtgcgcgtct tgaaaaattc
    28621 ttgggtggta tcgaagatat gcctcgtatc ccagatgtga tgtacgtagt tgacccacat
    28681 aaagagcaaa tcgctgttaa agaagctaaa aaattgggaa tcccagttgt agcgatggtt
    28741 gacaccaata ctgatccaga tgatatcgat gtaatcatcc cagctaacga tgacgctatc
    28801 cgtgctgtta aattgatcac agctaaattg gctgacgcta ttatcgaagg acgtcaaggt
    28861 gaggatgcag tagcagttga agcagaattt gcagcttcag aaactcaagc agattcaatt
    28921 gaagaaatcg ttgaagttgt agaaggtgac aacgcttaat ttatacaaat agtaattacc
    28981 taggagggcg gggcttagcc cggctctcct attttcaaaa aatataggag aattaaaatg
    29041 gcagaaatta cagctaaact tgtaaaagag ttgcgtgaaa aatctggtgc cggtgttatg
    29101 gacgctaaaa aagcgcttgt agaaacagac ggtgacatcg aaaaagcgat tgaattgctt
    29161 cgtgaaaaag gtatggctaa ggcagctaag aaagctgacc gtgttgctgc agaaggtttg
    29221 actggtgttt atgttaacgg taatgttgca gcagttattg aagtaaacgc tgaaactgac
    29281 ttcgttgcaa aaaacgctca attcgttgaa ttggtaaata ctacagctaa agtcattgct
    29341 gaaggaaaac ctgctaacaa cgaagaagct cttgctttga taatgccttc aggtgaaact
    29401 cttgaagctg catacgtatc tgcaacagca actatcggag agaaaatctc attccgtcgc
    29461 tttgcattga ttgaaaaaac agacgcacaa cactttggag cataccaaca taacggtgga
    29521 cgtatcggtg ttatttcagt tgttgaaggt ggagacgaag cacttgctaa acaattgtca
    29581 atgcacatcg cagcgatgaa accaacagtt ctttcttaca aagaattgga tgagcaattc
    29641 gttaaagatg agttggcaca attgaatcac gttatcgacc aagacaacga aagccgtgca
    29701 atggttaata aaccagctct tccacacttg aagtatggat caaaagctca attaactgat
    29761 gatgttattg ctcaagctga agctgacatc aaagctgaat tggctgcaga aggcaaacca
    29821 gaaaaaatct gggacaaaat tattccaggt aaaatggatc gcttcatgct tgataacact
    29881 aaagttgacc aagcttacac acttcttgca caagtttaca tcatggatga cagcaagaca
    29941 gttgaagcat accttgaatc agttaacgct tcggtagttg agtttgctcg ctttgaagtt
    30001 ggtgaaggta tcgagaaagc tgcaaacgac ttcgaagctg aagttgcagc tacaatggca
    30061 gcagccttga ataactaata atagaaagga agcaaattgc ttcctttttt gtatagggct
    30121 tagatttttc ctgaaattgt tttttcccca gtctcactcg attagaataa ttaaaacaaa
    30181 aagccctata ataagggcta agtgcattta ggagactata cttcaaattc atagtaagcg
    30241 cgtcataaca aggtatctat cctaattccc caacttccag tataccagat aaaaaataat
    30301 tatcggagga aaggtattgt actatgataa ttcaactaag tgatttaggt caagttcacc
    30361 ttgtttgtgg caagacagat atgaggcagg gaatagactc attagcctat gtagttaaaa
    30421 cccactttga attggatcct ttctccgatc aagtctttct cttttgtggt ggacgtaaag
    30481 accgctttaa agttctttac tgggatggtc aaggattttg gatattatat aaacgctttg
    30541 agaacggaaa actgacttgg tccagtacag aaaaggatgt caaagctctc acatctgaac
    30601 aagtagattg gctgatgaag ggcttttcta tcactcccca aatatagtgg atttaaacta
    30661 gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgactat cctgttctta
    30721 tttcatttta ctataaattc atcagaaagt cgtgatttct attgaaatga ggactttctt
    30781 tttatactca tctgttttca aaaagcattc tagtccgtct ccgattaacg atggacttta
    30841 tcacctcctt ctccagtcct tgtatgacat cttccaaagt tcgaaaggct ttattcttaa
    30901 atccacgttt acgaatctct ttccacactt gttcaatggg gttcatctct ggtgtgtatg
    30961 gaggaataaa tgcaaagcca atattagtcg gaatctttaa ggtacttgat ttatgccata
    31021 tagcattgtc cataacgagt aaaagataat catctggata agcttgtgaa agctcctatt
    31081 cctaaagccc ctttagcgca taactttggc tcagcttcta ttatcgctca caccatccat
    31141 cagaagttta atctgaaggt acccaattat cgccaagaag aagattgggc taggatgggt
    31201 ttaccaatca cacgtaagga aatctctaat tggcatatca agacgagtca atactatttg
    31261 gagccccttt ataacctctt gcgagagaga ctattgactc agcccttact tcatgcggat
    31321 gaaacttctt atagggtgct agagagtgat agtcagctga cttactattg gacttttttg
    31381 tctgggaaag ctgagaaaca agggattacg ctttaccatc atgttctaat cgatttgttc
    31441 atatcttatt tcaatccatt ataaatagtg agaaatatct atcctatctt ctagaatgtc
    31501 ttccaaacgt ggaaactctc gtaaacaaag aggttttaga ggcctattta ccatggacta
    31561 aagttgtaca agaaaagtgc aaataagaaa tctccagatt aggaactatc cgtgagttct
    31621 ctagtctgga gatttttcaa tagacttcgt tattggacgg ttacaattca taaagtgctg
    31681 tagagagata acgttcaccg ttatctggtg ctagggcaag gactttttta cctgtaccta
    31741 attttttggc aacctcgatg gctccgtaga tagctgcagc tgaggaaatc cctacaagga
    31801 agccttcttt tccaccaatt tcacgtccga gtgcaagagc gtcatctgat gttacacgaa
    31861 cgataccatc ataggcttta gtatcaagtg tatcaggaat aaatccagct gagatacctt
    31921 gaattttgtg aggaccaggt ttttcaccag atagaatagc agattcatct gcttctactg
    31981 caaaaacttg aatgttagaa ttttctgatt tgagtgcatg agaaacacca gaaatcgttc
    32041 caccagtacc tactccagca acaaaggcat ctaatccatc tttaccgaaa gcagctagta
    32101 tctcagctcc tgttgttctt tcgtgtactt ctggattagc tggattgtca aattgaagag
    32161 gaaggaaacc atcacgttca gcagcgattt cttgagcctt agcaatagca cctttcattc
    32221 cctcgctacc aggagttagg acgagttcag caccataagc ttggataatt ttacgtcgtt
    32281 ctacactcat agtttcaggc ataacgatga cgactttata ccctttagca gcacctaccc
    32341 atgaaagtcc aataccggtg tttccacttg ttgcttcaac aatagtagaa ccaggtttca
    32401 gaataccatc ttgttcagct ttttcaatca tgctaagggc aatacggtct tttacagatg
    32461 aaccaggatt aaatgcttca agctttatat agacgtctgc agcaccttct ggcacgatgt
    32521 tgttaagttt aacaatcggt gtttgaccga ttaattcagt aatgttgtta taaatagaca
    32581 tatgaatacc tctttgtata agatatcttt agtataacgc tatccatacc atttgtaaaa
    32641 tatagaaatc ctatcgaagc gataggattt ctatatatca tagatcaaag tcattaattt
    32701 tcaagtgatt gcgttcagct aattctagta aataggtgtg aaggaaaacg atatcaggtt
    32761 tcgtttgaat tgcttcattt tctagtatat tttggtacgc atctttttat tttgctcgct
    32821 aaagtagttg tgagaatgtc aacgaaacgc tgtattattc attgtttaca ctaagtttac
    32881 aggaacttca acctctcgta aaccttggtc agttaaagtg acttttccat taaaaaactc
    32941 cacaagtgca gctttaatag tttctttttc ttctttatca acataaatca tcgtatcgac
    33001 ttgatctgta aagtttgtat ccagctccat gagaccatgt tctttaagga agttactgta
    33061 ctcttggtac tgagcataag acatttgaat agcaatgcca gcctgttctt ttatttcaat
    33121 aataccaatt tctttgacag ctaaggcgac actgccggcg taagcacgaa ttagtcctcc
    33181 agcgcctagt ttaataccac caaagtagcg tgtcacgacc acacagacat tggtgagatt
    33241 gtgattttct agtaccccaa gcatgggaac accagcagta ccactaggct caccatcatc
    33301 acttgtacgt ttaatttcac tacgttctcc aataatgaag gcagagcagt tatgtgtcgc
    33361 tttgtagtgt tcttttttga tggcagtaat gaagtcacga gcctcttctt cgctataaac
    33421 acgcttggca tggcagataa agcgagattt tttgatttct tcttggactt gaccgtcctc
    33481 tttaattgtc ctaaattcca tgatttaatt gtactaaaaa atcatctaaa gtgctagttt
    33541 ttacgaataa agaagtatga aagtaaattt agattatctc ggtcgtttat ttactgagaa
    33601 tgaattaaca gaagaagaac gtcagttggc ggagaaactt ccagcaatga gaaaggagaa
    33661 ggggaaactt ttctgtcaac gctgtaatag tactattcta gaagaatggt atttgcccat
    33721 cggtgcttac tattgtcgag agtgcttgct gatgaagcga gtcagaagtg atcaaacttt
    33781 atactatttt ccgcaggagg attttccaaa gcaagatgtt ctcaaatggc gcggccaatt
    33841 aactcctttt caagagaagg tgtcagaggg attgcttcaa gtagtagaca agcaaaagcc
    33901 aaccttagtt catgcggtaa caggagctgg aaagacagaa atgatttatc aagtagtggc
    33961 taaagtgatc aatgcgggtg gtgcagtgtg tttggctagt cctcgcatag atgtttgttt
    34021 ggagctgtac aagcgcctgc aacaggattt ttcttgcggg atagctttgc tacatggaga
    34081 atcggaacct tattttcgaa caccactagt tgttgcaaca acccatcagt tattgaagtt
    34141 ttatcaagct tttgatttgc tgatagtgga tgaagtagat gcttttcctt atgttgataa
    34201 tcccatgctt taccacgctg tcaagaatag tgtaaaggag aatggattga gaatcttttt
    34261 aacagcgact tcgaccaatg agttagataa aaaggtccgt ttaggagaac taaaaagact
    34321 gaatttaccg agacggtttc atggaaatcc gttgattatt ccaaaaccaa tttggttatc
    34381 ggattttaat cgctacttag acaagaatcg tttgtcacca aagttaaagt cctatattga
    34441 gaagcagaga aagacagctt atccgttact catttttgct tcagaaatta agaaagggga
    34501 gcagttagca gaaatcttac aggagcaatt tccaaatgag aaaattggct ttgtatcttc
    34561 tgtaacagag gatcgattag agcaagtaca agcttttcga gatggagaac tgacaatact
    34621 tatcagtacg acaatcttgg agcgcggagt taccttccct tgtgtggatg ttttcgtagt
    34681 agaggccaat catcgtttgt ttaccaagtc tagtttgatt cagattggtg gacgagttgg
    34741 acgaagcatg gatagaccga caggagattt gcttttcttc catgatgggt taaatgcttc
    34801 aatcaagaag gcgattaagg aaattcagat gatgaataag gaggctggtc tatgaagtgc
    34861 ttgttatgtg ggcagactat gaagactgtt ttaactttta gtagtctctt acttctgagg
    34921 aatgatgact cttgtctttg ttcagactgt gattctactt ttgaaagaat tggggaagag
    34981 aactgtccaa attgtatgaa aacagagttg tcaacaaagt gtcaagattg tcaactttgg
    35041 tgtaaagagg gagttgaagt cagtcataga gcgattttta cttacaatca agctatgaag
    35101 gattttttca gtcggtataa gtttgatgga gacttcctgt taagaaaagt tttcgcttca
    35161 tttttaagtg aggagttgaa aaagtacaaa gagtatcaat ttgttgtaat tcccctaagt
    35221 cctgatagat atgctaatag aggatttaat caggttgagg gcttggtaga ggcagcaggc
    35281 tttgagtatc tggatttatt agagaaaaga gaagagagag ccagttcttc taaaaatcgt
    35341 tcagagcgct tggggacaga acttcctttc tttattaaaa gtggagtcac tattcctaaa
    35401 aaaatcctac ttatagatga tatctatact acaggagcaa ctataaatcg tgttaagaaa
    35461 ctgttggaag aagctggtgc taaggatgta aaaacatttt cccttgtaag atgaggaaaa
    35521 aatttgcaaa aatagaatga aagcgttata ataaaatcat aaaaacaaaa atgtttagaa
    35581 agaaggtact catatgatta aatatagtat ccgtggtgaa aacctagaag taacagaagc
    35641 aattcgtgat tatgtagttt ctaaactcga aaagatcgaa aagtacttcc aaccagaaca
    35701 agagttggat gcccgaatta acttaaaagt ttatcgtgaa aaaacggcta aagtggaagt
    35761 aacgattccg cttggatcta ttactcttcg cgcagaagat gtatctcaag atatgtatgg
    35821 ttcaattgac cttgtaactg ataaaattga acgtcagatt cgtaaaaata aaacaaaaat
    35881 cgagcgtaaa aataaaaata aggtagcaac tggtcaatta tttacagatg ctttggtgga
    35941 agattcaaat attgtccagt ctaaagttgt tcgttcaaaa caaattgatt taaaaccaat
    36001 ggatttggaa gaagcaattc tacaaatgga tttattgggg catgatttct ttatctatgt
    36061 ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt gaggatggcg aaattggttt
    36121 gttagaggtt aaagaatctt aatttcaata tgtgtaaagg taggtttact gaattgtaaa
    36181 ctaccttttt cttataattg atagaattac tgattacact tttaaaaagt cgctttttga
    36241 ggggtattat ggtataatgg gtgccaagag gttttgaatg aaaaaatttt atgtaagtcc
    36301 aatttttcct attctagtag gattgattgc gtttggagtc ttatccactt tcattatttt
    36361 tgttaataat aatctgttga cggttttaat tttgtttctt tttgtaggag gctatgtttt
    36421 tttatttaag aaactgagag tgcattatac aaggagtgat gtagaacaga tacagtatgt
    36481 aaaccaccaa gcggaagaaa gtttgacatc tctattggaa caaatgcctg taggtgttat
    36541 gaaattgaat ttatcttctg gagaggttga gtggtttaat ccctatgctg aattgatttt
    36601 gaccaaggaa gatggtgatt ttgatttaga agctgttcaa acgattatca aggcttcagt
    36661 aggaaatccg tctacttatg ccaagcttgg tgagaagcgt tatgctgttc atatggatgc
    36721 ttcttccggt gttttgtatt ttgtagatgt atccagggaa caagccataa cagatgaatt
    36781 ggtaacaagt cgaccagtga ttgggattgt ctctgtggat aattatgatg atttggagga
    36841 tgcaacttct gagtcagata ttagtcaaat caatagtttt gtagctaatt ttatatcaga
    36901 gttttcagaa aaatacatga tgttttctcg tcgggtaagt atggatcgat tttatctatt
    36961 tactgactac acggtgcttg agggcttgat gaatgataaa ttttctgtta ttgatgcttt
    37021 cagagaagag tcgaaacaga gacagttgcc cttgacctta agtatgggat tttcttatgg
    37081 cgatggaaat catgatgaga tagggaaagt tgctttgctc aatttgaact tggctgaagt
    37141 acgtggtggc gaccaggtgg ttgttaagga aaacgacgaa acgaaaaatc cagtttattt
    37201 tggtggtggg tctgctgctt caatcaagcg tacacggact cgtacgcgcg ctatgatgac
    37261 agctatttca gataagattc ggagtgtaga tcatgttttt gtagtcggtc acaaaaattt
    37321 agacatggat gctttgggct ctgctgtagg tatgcagttg ttcgccagca atgtgattga
    37381 aaatagctat gctctttatg atgaagaaca aatgtctcca gatattgaac gagctgtttc
    37441 attcatagaa aaagaaggag ttacgaagtt gttgtctgtt aaggatgcaa tggggatggt
    37501 gaccaatcgt tctttgttga ttcttgtaga ccattcaaag acagccttaa cattatcaaa
    37561 agaattttat gatttattta cccaaaccat tgttattgac caccatagaa gggatcagga
    37621 ttttccagat aatgcggtta ttacttatat cgaaagtggt gcaagtagtg ccagtgagtt
    37681 ggtaacggaa ttgattcagt tccagaattc taagaaaaat cgtttgagtc gtatgcaagc
    37741 aagtgtcttg atggctggta tgatgttgga tactaaaaat ttcacctcgc gagtaactag
    37801 tcggacattt gatgttgcta gctatctcag aacgcgcgga agtgatagta ttgctatcca
    37861 ggaaatcgct gcgacagatt ttgaagaata tcgtgaggtc aatgaactta ttttacaggg
    37921 gcgtaaatta ggttcagatg tactaatagc agaggctaag gacacgaaat gctatgatac
    37981 agttgttatt agtaaggcag cagatgccat gttagccatg tcaggtattg aagcgagttt
    38041 tgttcttgcg aagaatacac aaggatttat ctctatctca gctcgaagtc gtagtaaact
    38101 gaatgtacaa cggattatgg aagagttagg cggtggaggc cactttaatt tggcagcagc
    38161 tcaaattaaa gatgtaacct tgtcagaagc aggtgaaaaa ctgacagaaa ttgtattaaa
    38221 tgaaataaag gaaaaggaga aagaagaatg aaagtaatct ttttagcaga tgttaaagga
    38281 aaaggtaaaa aaggcgaaat taaggaagta ccaacagggt atgcgcaaaa ctttcttatc
    38341 aaaaagaatc tagccaaaga agcgactgct caagctgtag gtgaacttcg tggtaaacaa
    38401 aaatcggaag aaaaagctca cgctgagatg attgcagaag ggaaagcaat taaagcacaa
    38461 cttgaagcag aagaaactgt tgtagaattt gttgaaaaag ttggtccaga tggtcgtacc
    38521 tttggttcta ttaccaataa gaagattgca gaagaattac aaaagcaatt tggaattaag
    38581 attgataaac gtcatattca agtacaagct ccgattcgag cggttggttt gattgatgtg
    38641 ccagtgaaaa tctatcaaga tatcacaagt gtaatcaatc ttcgtgtgaa agaaggataa
    38701 gtttacacct tcttgacaag attgtaaaag gaagggaagt ctgatggcag aagtagaaga
    38761 gttacgagta caacctcaag atatcttagc tgagcaatcc gttttagggg ctatctttat
    38821 tgatgagagt aaacttgttt ttgtgcgaga atacattgag tctcgggact tttttaagta
    38881 tgcccatcgt ttgattttcc aagccatggt cgatttatcc gatcgtggtg atgccataga
    38941 tgcaacaacg gttcgtacta tccttgataa tcaaggtgat ttacagaata ttggtggctt
    39001 gtcttacttg gttgagattg tcaattctgt gccaacttct gctaatgcgg agtattatgc
    39061 taagattgtt gcagaaaaag caatgctacg tcgtttaatt gccaagttga cagagtctgt
    39121 caaccaagct tacgaagcgt cacaaccagc tgatgaaatt attgctcagg cagaaaaagg
    39181 gctgattgat gtcagtgaaa atgcaaatcg aagcgggttt aagaacattc gagatgtgtt
    39241 gaatctcaac tttggaaatc tggaagctcg ctcgcaacaa acgaccgata ttacaggtat
    39301 tgcgacaggt tatcgtgatt tggatcatat gacaacagga cttcatgagg aggagttgat
    39361 tatcttagca gctcgtccag cagttggtaa gacagcattt gccttgaata tcgctcagaa
    39421 tattgggact aagttggaca aaacggttgc tattttttca ctcgaaatgg gtgcggaaag
    39481 cttggtagac cgtatgttag ctgcagaagg cttggtggag tcacattcta tccgtacagg
    39541 gcaattgaca gatgaggagt ggcaaaaata tactattgct cagggtaatc tagctaacgc
    39601 cagtatctat atcgatgata cgccaggtat tcggattaca gagattcgtt ctcgttctcg
    39661 taaattggct caagaaactg gaaatcttgg tttgattttg atagactatt tgcaacttat
    39721 cacgggaact ggtcgagaaa atcgtcaaca agaagtttct gaaatttctc gtcagttgaa
    39781 aatactagcc aaggaattga aggttccagt aatcgctctg agtcagcttt ctcgtggtgt
    39841 agaacaacgt caggacaaga gaccggtctt gtctgatatt cgtgaatctg ggtctattga
    39901 gcaggacgct gatatcgtag cttttctcta tcgcgatgac tactatgaac gtggtggtga
    39961 agaagaggag ggtatcccaa ataataaggt ggaagttatt atcgagaaaa accgtagtgg
    40021 agctcgtgga acagtggaat tgattttcca aaaagaatac aataaatttt caagtatctc
    40081 aaagagggag gcataagatg tcagatgcat ttacagatgt agccaagatg aaaaaaatca
    40141 aagaagaaat caaggcacat gagggacaag tcgtagaaat gactttggag aatggtcgta
    40201 agcgccaaaa aaatagattg ggtaagctaa ttgaagttta tccatcccta tttattgtgg
    40261 agtttgggga tgtggaagga gataaacaag ttaatgttta cgttgaatcc tttacttact
    40321 cagatattct tacagaaaag aatttgattc attatcttga ctaaagtgag aaattttctc
    40381 actttttctt ttttctccga ataatttagg tgaaggcaat catcgcttta tattattttt
    40441 taaggaggaa gaatgaaaat tttaccgttt atagcaagag gaacaagtta ttacttgaag
    40501 atgtcagtta aaaagcttgt tcctttttta gtagtaggat tgatgctagc agctggtgat
    40561 agtgtctatg cctattccgg aggaaatgga tcgattgcgc gtggggatga ttatcctgct
    40621 tattataaaa atgggagcca ggagattgat cagtggcgca tgtattctcg tcagtgtact
    40681 tcttttgtag cctttcgttt gagtaatgtc aatggttttg aaattccggc agcttatgga
    40741 aatgcgaatg aatggggaca tcgtgctcgt cgggaaggtt atcgtgtaga taatacaccg
    40801 acgattggtt ccattacttg gtctactgca ggaacttatg gtcatgttgc ctgggtgtca
    40861 aatgtaatgg gagatcagat tgagattgag gaatataact atggttatac agaatcctat
    40921 aataaacgaa ttataaaagc aaacacgatg acaggattta ttcattttaa agatttggat
    40981 ggtggcagtg ttgggaatag tcaatcctca acttcaacag gcggaactca ttattttaag
    41041 accaagtctg ctattaaaac tgaaccccta gttagtgcaa ctgtgattga ttactattat
    41101 cctggagaga aggttcatta tgatcaaatt ctcgaaaaag acggctacaa gtggttgagt
    41161 tatactgcct ataatggaag ctatcgttat gttcaattgg aggctgtgaa taaaaatcct
    41221 ctaggtaatt ctgttctttc ttcaacaggt ggaactcatt attttaagac caagtctgct
    41281 atcaaaactg aacccctagt tagtgcaact gtgattgatt actattatcc tggggagaag
    41341 gttcattatg atcagatact tgaaaaagac ggctacaagt ggttgagtta tacggcttat
    41401 aacggaagtc gtcgctatat acagctagag ggagtgactt cttcacaaaa ttatcagaat
    41461 caatcaggaa atatctctag ctatggatcc aataatagtt caactgtcgg ttggaagaaa
    41521 ataaatggta gttggtatca tttcaaatca aatggttcta aatcaacagg atggctgaaa
    41581 gatggttcta gctggtatta tttgaaatta tctggtgaaa tgcagacagg atggttaaag
    41641 gagaatggct cgtggtatta tctgggtagt tcaggggcaa tgaaaacagg ctggtaccag
    41701 gtctctggta agtggtatta ttcttactct tcaggcgcct tagctgttaa tacgacggtg
    41761 gatggctaca gagtaaacag tgatggagaa cgagtataga aaattggagt aggagaaatt
    41821 tcctgctctt ttctgtaagc agtttccttg acatttttct tattttgcgc tatcatatat
    41881 acatataata tatgggagtc tgtgtacagt ctgagaggaa gtgttaaact tcgaccgcac
    41941 ctgatctggg taatgccagc ggagggaacg atacttagtc taattttgca ccttttccat
    42001 gtatggtaaa ggtttttctt tttttaaaaa ggaaaacgag aagaggaggt tcttatgaaa
    42061 gcaagcattg ccttgcaagt tttacccctg gcacagggga ttgatcggat agctgttatt
    42121 gatcaggtca ttgcttatct gcaaactcaa gaagtgacga tggtagtgac accatttgaa
    42181 acggtcttgg aaggggagtt tgatgagctt atgcgcattc taaaagaagc gctggaagtg
    42241 gcagggcagg aggcagacaa tgtctttgcc aatgtcaaaa taaatgtagg agagatttta
    42301 agtattgatg agaaacttga gaagtatact gagacgacac attagtctat tgggctttct
    42361 cggagtcttg tcaatctggc agttagcagg ttttcttaaa cttctcccca agtttatcct
    42421 gccgacacct cttgaaattc tccagccctt tgttcgtgac agagaatttc tctggcacca
    42481 tagctgggcg accttgagag tggctttact ggggctgatt ttgggagttt tgattgcctg
    42541 tcttatggct gtactcatgg atagtttgac ttggctcaat gacctgattt accctatgat
    42601 ggtggtcatt cagaccattc cgaccattgc catagctcct atcctggtct tgtggctagg
    42661 ttatgggatt ttgcccaaga ttgtcttgat tatcttaacg acaacctttc ccatcatcgt
    42721 cagcattttg gacggtttta ggcattgcga caaggatatg ctgaccttgt ttagtctgat
    42781 gcgggccaag ccttggcaaa tcctgtggca ttttaaaatt ccagtcagcc tgccctactt
    42841 ttatgctggt ctgagggtca gtgtctccta cgcttttatc acaacagtgg tatctgagtg
    42901 gttgggaggc tttgaggggc ttggtgttta tatgattcag tccaagaaat tgtttcagta
    42961 tgataccatg ttcgctatta ttattctggt atcgattatc agtcttttgg gtatgaagtt
    43021 ggtcgatatt agtgaaaaat atgttattaa atggaaacgt tcgtagaatt agaatgtttc
    43081 tgaaaaagaa aagaggaaat caaaatgaag aaaacatgga aagtgttttt aacgcttgta
    43141 acagctcttg tagctgttgt gcttgtggcc tgtggtcaag gaactgcttc taaagacaac
    43201 aaagaggcag aacttaagaa ggttgacttt atcctagact ggacaccaaa taccaaccac
    43261 acagggcttt atgttgccaa ggaaaaaggt tatttcaaag aagctggagt ggatgttgat
    43321 ttgaaattgc caccagaaga aagttcttct gacttggtta tcaacggaaa ggcaccattt
    43381 gcagtgtatt tccaagacta catggctaag aaattggaaa aaggagcagg aatcactgcc
    43441 gttgcagcta ttgttgaaca caatacatca ggaatcatct ctcgtaaatc tgataatgta
    43501 agcagtccaa aagacttggt tggtaagaaa tatgggacat ggaatgaccc aactgaactt
    43561 gctatgttga aaaccttggt agaatctcaa ggtggagact ttgagaaggt tgaaaaagta
    43621 ccaaataacg actcaaactc aatcacaccg attgccaatg gcgtctttga tactgcttgg
    43681 atttactacg gttgggatgg tatccttgct aaatctcaag gtgtagatgc taacttcatg
    43741 tacttgaaag actatgtcaa ggagtttgac tactattcac cagttatcat cgcaaacaac
    43801 gactatctga aagataacaa agaagaagct cgcaaagtca tccaagccat caaaaaaggc
    43861 taccaatatg ccatggaaca tccagaagaa gctgcagata ttctcatcaa gaatgcacct
    43921 gaactcaagg aaaaacgtga ctttgtcatc gaatctcaaa aatacttgtc aaaagaatac
    43981 gcaagcgaca aggaaaaatg gggtcaattt gacgcagctc gctggaatgc tttctacaaa
    44041 tgggataaag aaaatggtat ccttaaagaa gacttgacag acaaaggctt caccaacgaa
    44101 tttgtgaaat aatgacagaa attagactag agcacgtcag ttatgcctat ggtcaggaga
    44161 ggattttaga ggatatcaac ctacaggtga cttcaggcga agtggtttcc atcctaggcc
    44221 caagtggtgt tggaaagacc accctcttta atctaatcgc tgggatttta gaagttcagt
    44281 cagggagaat tgtccttgat ggtgaagaaa atcccaaggg gcgcgtgagt tatatgttgc
    44341 aaaaggatct gctcttggag cacaagacgg tgcttggaaa tatcattctg cccctcttga
    44401 ttcaaaaggt ggataaggca gaagctattt cccgagcgga taaaattctt gcgaccttcc
    44461 agctgacagc tgtaagagac aagtatcctc atgaacttag cggtgggatg cgccagcgtg
    44521 tagccttact ccggacctac ctttttgggc acaagctctt tctcttagat gaggccttta
    44581 gcgccttgga tgagatgaca aagatggaac tccacgcttg gtatcttgag attcacaagc
    44641 agttgcagct aacaaccctg atcatcacgc atagtattga ggaggccctc aatctcagcg
    44701 accgcatcta tatcttgaaa aatcgccctg ggcagattgt ttcagaaatt aaactagatt
    44761 ggtctgaaga tgaggacaag gaagtccaaa agattgccta caaacgtcaa attttggcgg
    44821 aattaggctt agataagtag aaaaataggg agttggtgaa gattatcctt taccagcgcc
    44881 ctttttcttt taaaaatgag aaaatttcgg tataatagtc aaacaaggtc aaggtttaaa
    44941 gagagaggtg ggtttgttat gagatttaaa aatacatcgg atcatattga ggcctacatc
    45001 aaggcgattt tagatcaatc tggtatcgtg gagttgcaac ggagtcagtt ggcagatacc
    45061 tttcaggttg ttcctagtca gattaactac gtgatcaaga cacgctttac ggaaagtaga
    45121 ggctacttgg ttgaaagtaa gcgtggtggc ggaggctaca ttcgtatagg acggattgag
    45181 ttttctagtc atcatgaaat gctccgggag ctgctttact cgattggtga gcgagtcagt
    45241 caagaaattt atgaggatat tctccagctt ttggttgagc aggaattgat gaccaagcag
    45301 gagatgaatt tgctagaatc agtagctttg gatcgcgttt taggagaaga agctccagtt
    45361 gttcgagcaa acatgctacg tcagatcata caagaggtag atagaaaagg gaagtaagat
    45421 gaactattca aaagcattga atgaatgtat cgaaagtgcc tacatggttg ctggacattt
    45481 tggagctcgt tatctagagt cttggcactt gttgattgcc atgtctaatc acagttatag
    45541 tgtagcaggg gcaactttaa atgattatcc gtatgagatg gaccgtttag aagaggtggc
    45601 tttggaactg actgaaacgg actatagcca ggatgaaacc tttacggaat tgccgttctc
    45661 ccgtcgtttg caggttcttt ttgacgaagc agagtatgta gcgtcagtgg tccatgctaa
    45721 ggtgctaggg acagagcacg tcttctatgc gattttgcat gatggcaatg ccttggcaac
    45781 tcgtatcttg gagagggctg gtttttctta tgaagacaag aaagatcagg tcaagattgc
    45841 tgcccttcgt cgaaatttag aagaacgggc aggctggact cgtgaagacc tcaaggcttt
    45901 acgccaacgc catcgtacag tagctgacaa gcaaaattct atggccaata tgatgggcat
    45961 gccgcagact cctagtggtg gtcttgagga ctatacgcat gatttgacag agcaagcgcg
    46021 ttctggcaag ttagaaccag tcatcggtcg ggacaaggaa atctcacgta tgattcaaat
    46081 tttgagccgt aagactaaga acaaccctgt cttggttggg gatgctggtg tcgggaaaac
    46141 agctctggcc cttggtcttg cccagcgtat tgctagtggt gacgtgcctg cggaaatggc
    46201 taagatgcgc gtgttagaac ttgatttgat gaatgtcgtt gcagggacac gcttccgtgg
    46261 tgactttgaa gaacgcatga ataatatcat caaggatatt gaagaagatg gccaagtcat
    46321 cctctttatc gatgaactcc acaccatcat gggttctggt agcgggattg attcgactct
    46381 ggatgcggcc aatatcttga aaccagcctt ggcgcgtgga actttgagaa cggttggtgc
    46441 caccactcag gaagaatatc aaaaacatat cgaaaaagat gcggctcttt ctcgtcgttt
    46501 cgctaaagtg acgattgaag aaccaagtgt ggcagatagt atgactattt tacaaggttt
    46561 gaaggcgact tatgagaaac atcaccgtgt acaaatcaca gatgaagcgg ttgaaacagc
    46621 ggttaagatg gctcatcgtt atttaaccag tcgtcacttg ccagactctg ctatcgacct
    46681 cttggacgaa gcagcagcaa cagtgcaaaa taaggcaaag catgtaaaag cagacgattc
    46741 agatttgagt ccagctgaca aggccctgat ggatggcaag tggaaacagg cagcccagct
    46801 aatcgcaaaa gaagaggaag tacctgtcta caaagacttg gtgacagagt ctgatatttt
    46861 gaccaccttg agtcgcttgt caggaatccc agttcaaaaa ctgactcaaa cggatgctaa
    46921 gaagtattta aatcttgaag cagaactcca taaacgtgtc atcggtcaag atcaagctgt
    46981 ttcaagtatt agtcgtgcca ttcgccgtaa tcagtcagga attcgcagtc acaagcgtcc
    47041 gatcggttcc tttatgttcc taggacccac aggtgtcggt aagaccgagt tggccaaggc
    47101 tttggcagaa gttctctttg atgacgaatc agcccttatc cgctttgata tgagtgagta
    47161 tatggagaaa ttcgcagcca gccgtctaaa tggagctcct ccaggctatg tgggttacga
    47221 agaaggtggg gagctgacag agaaggttcg caataaaccc tattccgttc tcctctttga
    47281 tgaggtagag aaggcccacc cagatatctt taatgttctc ttgcaggttc tggatgacgg
    47341 tgtcttgaca gatagcaagg gacgcaaggt cgatttttca aataccatta tcattatgac
    47401 atcgaatcta ggtgcgactg cccttcgtga tgataagact gttggttttg gggctaagga
    47461 tattcgtttt gaccaggaaa atatggaaaa acgcatgttt gaagaactga aaaaagctta
    47521 tagaccggaa ttcatcaacc gcattgatga gaaggtggtc ttccatagcc tatctagtga
    47581 ccatatgcag gaagtggtga agattatggt caagccttta gtggcaagtt tggctgaaaa
    47641 aggcattgac ttgaaattac aagcttcagc tctgaaattg ttagcaaatc aaggatatga
    47701 cccagagatg ggagctcgcc cacttcgcag aaccctgcaa acagaagtgg aggacaagtt
    47761 ggcagaactt cttcttaagg gagatttagt ggcaggcaac acacttaaga ttggtgtcaa
    47821 agcaggccag ttaaaatttg atattgcata aaagaataaa agtatcagca tctgaccata
    47881 agtcacagtg gagtgaaact caatgaaaat caaagagcaa actaggcagc tagctgcagg
    47941 ttgctcaaaa cactgttttg aggttgcaga tagagctgac gtggtttgaa gagattttcg
    48001 aagagtatga aactaaaacc tatagcttct aaacgatccg tggttttcac cattcaacac
    48061 aaaattcata tgtttattac cctccgtcgt atttgtctta gagcgtgtgt agtagaaaaa
    48121 gagcagtctt atctgaaatt tttattcttt caaaagagac ctgtttcttt tttgcatgtc
    48181 aaatccgttc tagctggtat ttgaaaaatc aaactaatat tcaatgaaaa tcaaagaaca
    48241 aactaggaag ctagccgcag gttgctcaaa acactgtttt gaggttgtag atagagctga
    48301 cgtggtttga agagattttc gaagagtata agctgcaaga tgaatgattt tcttgtattg
    48361 acgttgttgt tgacaaaaag tagcggataa atgaaatcca ttccattatc atagatgata
    48421 ggctggtagg aaattttcaa atagcataca ggaaatagat gtatggagtt ctggtagtag
    48481 aaagggagag agatgaacat tttagttgca gatgacgagg aaatgattag agaaggaatt
    48541 gcagcatttc tgacagaaga gggttatcat gtcattatgg ctaaggatgg acaagaggtc
    48601 ttggaaaaat ttcaagatct ccctatccat ctcatggtac tggatttaat gatgcctagg
    48661 aagagtggtt ttgaagtgtt aaaagaaatc aatcaaaagc acgatattcc tgtcatcgtc
    48721 ttgagtgctc tgggagatga aactactcag tcacaggtat ttgatctcta tgctgatgat
    48781 catgtgacaa aacctttttc tttggtactg cttgtcaagc gtattaaggc gcttatcaga
    48841 cgttactacg tcatagagga tctttggcga tatcaggatg taacagtgga ttttacctct
    48901 tacaaagcac attataaaaa tgaagaaatt gatctcaaac caaaggaatt actggtacta
    48961 aagtgtttga ttcagcataa aaatcaagtt ttaagtagag agcagatatt ggaagaaatt
    49021 tcaaaagatg tagctgattt accttgtgat agggtcgttg atgtctatat tcgtactctt
    49081 cgcaaaaaat tagctttaga ttgtatcgtg actgtgaaaa atgttgggta taagattagc
    49141 ttatgataaa aaatcctaaa ttattaacca agtctttttt aagaagtttt gcaattctag
    49201 gtggtgttgg tctagtcatt catatagcta tttatttgac ctttcctttt tattatattc
    49261 aactggaggg ggaaaagttt aatgagagcg caagagtgtt tacggagtat ttaaagacta
    49321 agacatctga tgaaattcca agcttactcc agtcttattc aaagtccttg accatatctg
    49381 ctcaccttaa aagagatatt gtagataagc ggctccctct tgtgcatgac ttggatatta
    49441 aagatggaaa gctatcaaat tatatcgtga tgttagatat gtctgttagt acagcagatg
    49501 gtaaacaggt aaccgtgcaa tttgttcacg gggtagatgt ctacaaagaa gcaaagaata
    49561 ttttgctttt gtatctccca tatacatttt tggttacaat tgctttttcc tttgtttttt
    49621 cttattttta tactaaacgc ttgctcaatc ctctttttta catttcagaa gtgactagta
    49681 aaatgcaaga tttggatgac aatattcgtt ttgatgaaag taggaaagat gaagttggtg
    49741 aagttggtga agttggaaaa cagattaatg gtatgtatga gcacttgttg aaggttattc
    49801 atgagttgga aagtcgtaat gagcaaattg taaaattgca aaatcaaaag gtttcctttg
    49861 tccgcggagc atcacatgag ttgaaaaccc ctttagccag tcttagaatt atcctagaga
    49921 atatgcagca taatattgga gattacaaag atcatccaaa atatattgca aagagtataa
    49981 ataagattga ccagatgagc cacttattgg aagaagtact ggagtcttct aaattccaag
    50041 agtggacaga gtgtcgtgag accttgactg ttaagccagt tttagtagat attttatcac
    50101 gttatcaaga attagctcat tcaataggtg ttacaattga aaatcaattg acagatgcta
    50161 ccagggtcgt catgagtctt agggcattgg ataaggtttt gacaaacctg attagtaatg
    50221 caattaaata ttcagataaa aatgggcgcg taatcatatc cgagcaagat ggctatctct
    50281 ctatcaaaaa tacatgtgcg cctctaagtg accaagaact agaacattta tttgatatat
    50341 tctatcattc tcaaatcgtg acagataagg atgaaagttc cggtttgggt ctttacattg
    50401 tgaataatat tttagaaagc tatcaaatgg attatagttt tctcccttat gaacacggta
    50461 tggaatttaa gattagcttg tagacagatt agttttttat taaagttcat atagggttaa
    50521 cataagtgtg ttattctttg tgtagataaa agaaaggata ctaatatggt attagcgatt
    50581 attttagtaa cattccttat tcgattgatt tttttaaagc gttcgataga gaatgagaaa
    50641 cgaatcctta gcaatggcgg gaaagaattt ggagttgaga atacaaaacg attaactatg
    50701 gctcatattg ttttttatct ctcttgcttg gttgaggcaa tggtgcacaa gacaattttt
    50761 gatagcatgg gcatggttgg tttagtcttg cttatttttt ctatgctgat gttaatgttg
    50821 gtgattcact tgttgggaga tatttggaca gtgaagctta tgcttgtcaa taatcacaaa
    50881 tatgtagatc atatcttgtt taggacagta aaacatccta attacttttt aaatattctt
    50941 cctgagttga ttggcttgac cttgttgagt catgcctata tgacttttgt tttagttttt
    51001 ccagtttatg cagttatttt gtatcgacga atagctgaag aggaaaagct attacatgaa
    51061 gttataatcc caaatggaag cataaagaga taaatacaaa attcgattta tatacagttc
    51121 atattgaagt gatatagtaa ggttaaagaa aaaatataga aggaaataaa catgtttgca
    51181 tcaaaaagcg aaagaaaagt acattattca attcgtaaat ttagtattgg agtagctagt
    51241 gtagtagttg ctagcttgtt cttaggagga gtagttcatg cagaagaggt tggtggtagg
    51301 aataccccca cggttacatc tagtgggcaa gatatatcga agaagtatgc tgatgaagtc
    51361 gagtcgcatc taaaaaaaat attgagtgag atccaaacac aattagatag aaaacgacat
    51421 accgaaactg tagcgctaat taacgagttg caaggcatta agaaaacgta tttgtataat
    51481 ttaaatgttt taaaagagaa gtcgaaagtt gagttgccgt caaaaataaa agcaaagtta
    51541 gacgtagctt ttgatcagtt taaaaaagat acattgaaac caggagaaaa ggtagcagaa
    51601 gctcagaaga aagttgcaga agctaagaaa aaagccgagg atcaaaaaga agaagatcgc
    51661 cgtaactacc caaccaatac ttacaaaacg cttgaacttg acattgctga gtccgatgtg
    51721 aaagttaaag aagcggagct tgaactagta aacgaggaag ctaaacctcg aaacgaggaa
    51781 aaaattaaga aagcaaaagc gaaagttgag agtgaaaaag ctgaggctat aaggttagaa
    51841 gaaatcaaga cagatcgtga agaagctaaa cgaaaagcag atgctaagtt gaaggaagct
    51901 gttgaaaaga atgcagcgaa ttcagagcaa ggtgaaccaa agaggcgggt aaaacgagga
    51961 gttcttggag agccagcaac acctgataaa aaagaaaatg atgcgaagtc ttcagattct
    52021 agcgtaggtg aagaaactct tccaagccca tccctgaaac cagaaaaaaa ggttgcagaa
    52081 gctgagaaga aggttgaaga agctaagaaa aaagccgagg atcaaaaaga agaagatcgc
    52141 cgtaactacc caaccaatac ttacaaaacg cttgaacttg aaattgctga gtccgatgtg
    52201 aaagttaaag aagcggagct tgaactagta aacgaggaag ctaaacctcg aaacgaggaa
    52261 aaaattaaga aagcaaaagc gaaagttgag agtgaaaaag ctgaggctac aaggttagaa
    52321 aaaatcaaga cagatcgtaa aaaagcagaa gaagaagcta aacgaaaagc agcagaagaa
    52381 gataaagtta aagaaaaacc agctgaacaa ccacaaccag cgccggctcc tcaaccagaa
    52441 aaaccagctg aagagcctaa tccagctcca gctccaaaac cagagaagcc agctgatcaa
    52501 ccaaaagcag aaaaaccagc tgatcaacaa gctgaagaag actatgctcg tagatcagaa
    52561 gaagaatata atcgcttgac tcaacagcaa ccgccaaaac cagagcaacc agctcctgca
    52621 ccaaaaacag gctggaaaca agaaaacggt atgtggtact tctacaatac tgatggttca
    52681 atggcgacag gatggctcca aaacaatggc tcatggtact acctcaacag caatggcgct
    52741 atggcgacag gatggctcca atacaatggt tcatggtact acctcaacgc taatggtgat
    52801 atggcgacag gatggttcca atacaatggt tcatggtact acctcaacgc taatggtgat
    52861 atggcgacag gatggctcca atacaatggt tcatggtact acctaaacag caatggtgct
    52921 atggtaacag gatggctcca aaacaatggc tcatggtact acctaaacgc taacggttca
    52981 atggcaacag attgggtgaa agatggagat acctggtact atcttgaagc atcaggtgct
    53041 atgaaagcaa gccaatggtt caaagtatca gataaatggt actatgtcaa tggctcaggt
    53101 gcccttgcag tcaacacaac tgtagatagc tatagagtca atgccaatgg tgaatgggta
    53161 aactaaactt aatataacta gttaatactg acttcctgta agaactcttt aaagtattcc
    53221 ctacaaatac catatccttt cagtagataa tatacccttg taggaagttt agattaaaaa
    53281 ataactctgt aatctctagc cggatttata gcgctagaga ctacggagtt ttttttggct
    53341 ctttgtcaac tgtagtgggt tgaagtcagc taagctcgag aaaggacaaa ttttgtcctt
    53401 tcttttttga tattcagagc gataaaaatc cgttttttga agttttcaaa gttccgaaaa
    53461 ccaaaggcat tgcgcttgat aagtttgatg agattattgg tcgcttccaa tttggcgtta
    53521 gaatagtgta gttgaagggc gttgacgatt ttctctttgt cctttagaaa ggttttaaag
    53581 acagtctgaa aaagaggatg aacctgcttt agattgtcct caatgagtcc gaaaaatttc
    53641 tccggtt
//
LOCUS       DAVKHZ010000006        49109 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000006 DAVKHZ010000000
VERSION     DAVKHZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.006"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            183..2198
                     /locus_tag="VWW99_000298"
     CDS             183..2198
                     /locus_tag="VWW99_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9538196.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWFIFLRPVGEDLKAIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGEGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEVGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            2200..2832
                     /locus_tag="VWW99_000299"
     CDS             2200..2832
                     /locus_tag="VWW99_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538197.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            2973..3251
                     /locus_tag="VWW99_000300"
     CDS             2973..3251
                     /locus_tag="VWW99_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEU9538198.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(3760..4173)
                     /gene="ndk"
                     /locus_tag="VWW99_000301"
     CDS             complement(3760..4173)
                     /gene="ndk"
                     /locus_tag="VWW99_000301"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9538199.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(4284..7961)
                     /gene="rpoC"
                     /locus_tag="VWW99_000302"
     CDS             complement(4284..7961)
                     /gene="rpoC"
                     /locus_tag="VWW99_000302"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9538200.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(7994..11605)
                     /gene="rpoB"
                     /locus_tag="VWW99_000303"
     CDS             complement(7994..11605)
                     /gene="rpoB"
                     /locus_tag="VWW99_000303"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9538201.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            12021..12091
                     /locus_tag="VWW99_000304"
     tRNA            12021..12091
                     /locus_tag="VWW99_000304"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12053..12055,aa:Cys,seq:gca)
     gene            complement(12289..13620)
                     /locus_tag="VWW99_000305"
     CDS             complement(12289..13620)
                     /locus_tag="VWW99_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9538202.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(13911..14735)
                     /gene="endA"
                     /locus_tag="VWW99_000306"
     CDS             complement(13911..14735)
                     /gene="endA"
                     /locus_tag="VWW99_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9538203.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(14774..14962)
                     /locus_tag="VWW99_000307"
     CDS             complement(14774..14962)
                     /locus_tag="VWW99_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9538204.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(14955..16238)
                     /gene="murA"
                     /locus_tag="VWW99_000308"
     CDS             complement(14955..16238)
                     /gene="murA"
                     /locus_tag="VWW99_000308"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9538205.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(16262..16492)
                     /locus_tag="VWW99_000309"
     CDS             complement(16262..16492)
                     /locus_tag="VWW99_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9538206.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(16550..17587)
                     /locus_tag="VWW99_000310"
     CDS             complement(16550..17587)
                     /locus_tag="VWW99_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9538207.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(17571..18059)
                     /gene="coaD"
                     /locus_tag="VWW99_000311"
     CDS             complement(17571..18059)
                     /gene="coaD"
                     /locus_tag="VWW99_000311"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9538208.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(18049..18588)
                     /gene="rsmD"
                     /locus_tag="VWW99_000312"
     CDS             complement(18049..18588)
                     /gene="rsmD"
                     /locus_tag="VWW99_000312"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9538209.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(18653..19645)
                     /locus_tag="VWW99_000313"
     CDS             complement(18653..19645)
                     /locus_tag="VWW99_000313"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9538210.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(19757..20440)
                     /locus_tag="VWW99_000314"
     CDS             complement(19757..20440)
                     /locus_tag="VWW99_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9538211.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(20496..21236)
                     /locus_tag="VWW99_000315"
     CDS             complement(20496..21236)
                     /locus_tag="VWW99_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9538212.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            21270..21548
                     /locus_tag="VWW99_000316"
     CDS             21270..21548
                     /locus_tag="VWW99_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9538213.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            21626..22552
                     /gene="yidC"
                     /locus_tag="VWW99_000317"
     CDS             21626..22552
                     /gene="yidC"
                     /locus_tag="VWW99_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9538214.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(22627..23421)
                     /gene="pflA"
                     /locus_tag="VWW99_000318"
     CDS             complement(22627..23421)
                     /gene="pflA"
                     /locus_tag="VWW99_000318"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9538215.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(23547..24797)
                     /locus_tag="VWW99_000319"
     CDS             complement(23547..24797)
                     /locus_tag="VWW99_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9538216.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERAAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(24864..25691)
                     /gene="purR"
                     /locus_tag="VWW99_000320"
     CDS             complement(24864..25691)
                     /gene="purR"
                     /locus_tag="VWW99_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9538217.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(25958..26899)
                     /locus_tag="VWW99_000321"
     CDS             complement(25958..26899)
                     /locus_tag="VWW99_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9538218.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(26889..28145)
                     /locus_tag="VWW99_000322"
     CDS             complement(26889..28145)
                     /locus_tag="VWW99_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9538219.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            complement(28147..28809)
                     /locus_tag="VWW99_000323"
     CDS             complement(28147..28809)
                     /locus_tag="VWW99_000323"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9538220.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKGAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(28772..29428)
                     /locus_tag="VWW99_000324"
     CDS             complement(28772..29428)
                     /locus_tag="VWW99_000324"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9538221.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(29439..30317)
                     /gene="rsgA"
                     /locus_tag="VWW99_000325"
     CDS             complement(29439..30317)
                     /gene="rsgA"
                     /locus_tag="VWW99_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9538222.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKKELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(30319..31191)
                     /gene="rsmA"
                     /locus_tag="VWW99_000326"
     CDS             complement(30319..31191)
                     /gene="rsmA"
                     /locus_tag="VWW99_000326"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9538223.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMTFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(31577..31735)
                     /locus_tag="VWW99_000327"
     CDS             complement(31577..31735)
                     /locus_tag="VWW99_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538224.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQILVHGYL
                     LDFASIEG"
     gene            complement(31728..32369)
                     /locus_tag="VWW99_000328"
     CDS             complement(31728..32369)
                     /locus_tag="VWW99_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538225.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(32371..34401)
                     /locus_tag="VWW99_000329"
     CDS             complement(32371..34401)
                     /locus_tag="VWW99_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9538226.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQGLFTDYL
                     QNFSSESVEVTSQKYYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(34670..35569)
                     /locus_tag="VWW99_000330"
     CDS             complement(34670..35569)
                     /locus_tag="VWW99_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9538227.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(35641..36201)
                     /gene="rnmV"
                     /locus_tag="VWW99_000331"
     CDS             complement(35641..36201)
                     /gene="rnmV"
                     /locus_tag="VWW99_000331"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9538228.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(36201..36974)
                     /locus_tag="VWW99_000332"
     CDS             complement(36201..36974)
                     /locus_tag="VWW99_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9538229.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(37279..38160)
                     /locus_tag="VWW99_000333"
     CDS             complement(37279..38160)
                     /locus_tag="VWW99_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9538230.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLV
                     VATSGFALLGRKTRRRK"
     gene            complement(38444..38578)
                     /gene="rpmH"
                     /locus_tag="VWW99_000334"
     CDS             complement(38444..38578)
                     /gene="rpmH"
                     /locus_tag="VWW99_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9538231.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(38723..39937)
                     /locus_tag="VWW99_000335"
     CDS             complement(38723..39937)
                     /locus_tag="VWW99_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9538232.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(40093..40386)
                     /locus_tag="VWW99_000336"
     CDS             complement(40093..40386)
                     /locus_tag="VWW99_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538233.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            40548..41000
                     /locus_tag="VWW99_000337"
     CDS             40548..41000
                     /locus_tag="VWW99_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9538234.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(41035..42423)
                     /locus_tag="VWW99_000338"
     CDS             complement(41035..42423)
                     /locus_tag="VWW99_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9538235.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDMLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            42489..43451
                     /locus_tag="VWW99_000339"
     CDS             42489..43451
                     /locus_tag="VWW99_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9538236.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(43657..44667)
                     /gene="ccpA"
                     /locus_tag="VWW99_000340"
     CDS             complement(43657..44667)
                     /gene="ccpA"
                     /locus_tag="VWW99_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9538237.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(45092..45691)
                     /locus_tag="VWW99_000341"
     CDS             complement(45092..45691)
                     /locus_tag="VWW99_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538238.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(45693..46790)
                     /locus_tag="VWW99_000342"
     CDS             complement(45693..46790)
                     /locus_tag="VWW99_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9538239.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(46790..47527)
                     /locus_tag="VWW99_000343"
     CDS             complement(46790..47527)
                     /locus_tag="VWW99_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538240.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(47529..48413)
                     /locus_tag="VWW99_000344"
     CDS             complement(47529..48413)
                     /locus_tag="VWW99_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538241.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(48400..48594)
                     /locus_tag="VWW99_000345"
     CDS             complement(48400..48594)
                     /locus_tag="VWW99_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538242.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(48591..48797)
                     /locus_tag="VWW99_000346"
     CDS             complement(48591..48797)
                     /locus_tag="VWW99_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538243.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        14137 a        11243 c         9344 g        14385 t
ORIGIN      
        1 aaggaaaaag aaaggggttt cattgcatga gaaaaaaacg tggaattaaa aaattagtta
       61 catttgatgc gggatggtaa agaataattg aagaagattc ttttatcaat agtttagtaa
      121 gcttgttagt ttatagaggg caggggtatt tctgccctct gttttagaat aaggaatgca
      181 ctatgaaaaa attatttata ctcatatcca acctgcttgc tagtttgttc tttgtttggg
      241 tatttaccat ttggactgat acatatgtct cccattatta ccccaacgtt gttgtacgtg
      301 attcttcccc tgaaacaact tttcagcatg ttgctacacg cttggaaaaa ctagcagaag
      361 aaacagatag ctttattgcg attcaacacc aagatcctaa ctcagaaggt actacagttt
      421 tttcttatac gacctttggg gacggaaagt tgcctgatgg gcttcaggaa aaaaatctag
      481 aagatgctca aagtagtagt gttgaaacaa actattttgt attcgatgga cacctagata
      541 ttcacttgct aagggaagag ctaagtcaac ttggcttgac taacatgcac ctgacaattc
      601 catctaaact atctacgtta atggctatct ttagtaacgg atttcagtta atcagtctat
      661 taattttcat cttaaccttt gtagcactga ctttaattag tcaaattagc caattacggt
      721 catcgggcat tcgcctcatt tcaggagaga aacggtggtt cattttcctt agaccagttg
      781 gtgaggattt gaaagcgata gctgttggtt tcagtttagc tggcgtgctc gccattctta
      841 tgcaaaaaat tctgtcgctt ccaacgcaat ccctaatgac cataggagaa ggcttgctca
      901 gttataatct catcttgttg tcgatctccc tgtttttcgc tcaactcttc gcagttggga
      961 taaaaaagat acacctgatg cagattataa aagggcaagt gcctgtcaga ggaatcatta
     1021 gtttgatttt aatcggtcaa ctattagcga ttattattgt aacgctggga atagggagta
     1081 gcttaaagta ttcccaagcc tggcaacagc atcggattgg acaagaggct tggagtcagg
     1141 aaagacaact gattacccta tcaatcagcc gtgaaggaac gagtcctggt tttgatgaac
     1201 aagctcaaag gaaactcaga acttggtatc aattgatgga tctggctgtt tcagaacaaa
     1261 aggctttctt atctagacac cagttaattg accgtacttt gcaaaatggc atggcttcct
     1321 ccaaaaactt gataacctct acagaatggc acgattacaa cccgaatggc aatgtcctta
     1381 tcgtcacacc gcaatacttg gaacgtcaaa acattcctgt agatacaact attgagcaaa
     1441 agatgaatca ccttaatgta ggggagtttg tcttattgct gcctgaacac ctccgttcag
     1501 aggaagaaca ttataaatct gtttttgaag acgacttaac cagtcgcatg tctagtcaag
     1561 atgaacgaca gcaaatgact gctacggtag gttatttaga atcaggtcag gatcgttttg
     1621 tgtataatac gacccctatt tcttaccagc agtttttgaa agatccaatc atcattgtta
     1681 taacacccca atcaactggt ccacagtcca ttttgttttg gatagacgca gtacagaact
     1741 acgttctctt taatcaattg tctgatgccc aggagcttat ccagagacaa ggcattgaaa
     1801 attgggtctc agaaatgcaa acaggttacc acaactacat cacattattg gataatatcc
     1861 agagggaacg ttgggtaatg ctagcaggag ctgtgcttgg gattgcaact tcaatcttgt
     1921 tgtttaacac tatgaatagg ctctactttg aagaatttag acgtgccatt tttatcaaac
     1981 gcattgcagg tctcaggttc ttagaaatcc atcgcactta tctctttgct caactgggtg
     2041 tgtttttact gggatttgtt gcgagtgtat ttcttcaggt agaggtagga gttgctttct
     2101 tagtcttgtt actctttact ggtctatctc ttttacagtt acatgtccaa atgcagaaag
     2161 aaaacaagat gtccatgctt gttttgaagg gaggttaata tgattgaact taaacaggtg
     2221 agtaaatctt ttggagaacg agagttattt tcgaatcttt caatgacatt tgaggctgga
     2281 aaagtctatg ccttaattgg ttcaagtggt agcggaaaaa caaccttgat gaacatgatt
     2341 gggaaattag aaccttatga tgggacgatt ttttaccgag gtaaagactt ggccaattat
     2401 aaatcgagtg attttttccg tcacgaattg ggctacctct tccagaactt tggcttaatt
     2461 gaaaaccaaa gtattgaaga aaaccttaag ctaggtctca ttggtcaaaa gttgagtcgg
     2521 tcggaacagc ggttgaggca gaagcaggct ttagaacagg tcggcctggc ttatcttgac
     2581 ctagataagc gcatctttga gttatcgggc ggagaatcgc aacgagttgc cttggcaaaa
     2641 attatcttaa agaatccacc ctttattctg gcagatgagc caacagcttc aatagaccca
     2701 gcaacctctc agttgattat ggagattttg ctatctcttc gagatgataa taggctaatc
     2761 attatcgcaa cacataatcc ggcaatttgg gagatggctg atgaagtgtt cacgatggat
     2821 catctgaaat aaaaatcctt gtttttaatt gcacgatgag ttactgaaat attatcatga
     2881 atcaataatt ggagttaatt tagaattgta ctttattaat attgaggtaa ctttttcttg
     2941 ataaaggagg aaatagtgga gaggaagtta gaatgaaaaa attcgacaat tatattattg
     3001 agaagccttg cgattctaat tcagataaac tgcaaaaaat cttaataatt gaaaatttgg
     3061 tagatgatat tttgcaattt cctctcagaa tcaataatag tgtaggagag attttcctcc
     3121 tacaaccgtt tcaaaagaaa actatcttta ttccatgtta ttttgaggaa gatattgtga
     3181 aagtcaaaga tgatgataaa gttgagtgga atttgttaga atttcaaaaa tttagagcat
     3241 ttttggctta gtaatctgtg ttgaaggctc aaaacctatg gtaaaaaagt cgctttgaaa
     3301 acgtattgcc tccaaagatt tagttaaata atgatttaac acaaaaagaa attattgaag
     3361 ttctggaaag atgttgtttc agtattgaga aaaggtggga aaaacttgcg attttcacag
     3421 agaaaggaag aaaaagtata gaaatatagt caattgaaac aagaacagga caaaagaacc
     3481 ttttgtgcca tatttttctc ctttcgcttt acaattggct tgaacacctt tattgtatcg
     3541 cgtttggagt ttttttggta taaccttcga cgcgcacccg catagcgggt gtttttttgt
     3601 ctcgcaccta acggagcgag acgaactaat agtcacttaa tcaaaaaacg caccatatca
     3661 aaaactaaaa agtttgatat catgcgtcat gtcttaaact aattgactat actttctatt
     3721 caaatgagct tttaaccaat tgattgagcc aatccactct taaaaccaaa gagcaatttc
     3781 tcgcttagct gactcttctg aatctgaacc atgtacaaca ttttggataa cctcattttc
     3841 tccagcagct tttgcaaagt cacctcgaat agtgcctggt aaagcttctt ctggacgagt
     3901 tgcacccatc atggtccgcc aagtttcgat tactttggga ccagaaatga cacccacaag
     3961 aactggacct gaagtcatga attcacgaat cggtaggtaa aaactctgac caaccaagtc
     4021 ctgatagtgc tggtcaatca actcttctga aacctgtgaa cgaaactcca atttttcgat
     4081 tgtaaatcca cgttgttcga tgcgctttaa cacttcaccc actagccctc tttttacacc
     4141 atctggtttg atgataaaga atgtttgttc catacccgtc tcctttgtca gcttctttct
     4201 tttattttac cacatttcgt ggaaaaatgg agaaagtttt cagaagagag aatgagagaa
     4261 cccgagggtt ctctcattct ctcttattct actgtttctt ccacagtttc aacggcagta
     4321 tccacaacta cttctgttgt ttcttcattt ccttcttcct ctactggagg attaaggtat
     4381 tcttcttcgt tgacagcatg tggttcaagg ttacggtaac gggccatacc agtaccagct
     4441 gggatgatct taccgatgat aacattttct ttaagtccaa ggagatggtc tttcttacca
     4501 cggatagctg cgtcagtaag gacacgagtt gtttcttgga aggaagctgc tgacaagaaa
     4561 ctgtttgttt caagtgaggc tttggtaatt cccataagga ctgggcgacc tgtcgctgga
     4621 actccacctg cgataaggac atctttgttg gcatctgtaa agtcattgat atccatgagg
     4681 gtacccatga gaagatctgt atcacctgga tccatgacac ggactttacg gatcatttga
     4741 cgaaccatta cctcgatgtg tttgtcaccg atttctaccc cttggctacg gtaaactttt
     4801 tgtacttcac cgagaaggta cgtttcaact gacaagacat cacgaactgc aaggagacgt
     4861 tttggttgga tagaaccttc tgtcagagca gcaccacgcg ctacttggcc cccaacttcg
     4921 acacgcatac gagctgtaaa tggaacgaca tattcacctt cgccagtttc acccttaaca
     4981 aagactttct tggtacgagt tgatgcatct tcttcgatag cagtaacttg tcctttaacc
     5041 tctgtgataa ccgcttcccc tttaggatta cgggcttcaa agatttcttg gacacgagga
     5101 agaccctgag tgatatcggt atttgaggca accccacctg tgtggaaggt acgcattgta
     5161 agctgtgtac caggttcccc gatagattgg gcagcaattg taccaactgc ttcaccaact
     5221 tcaaccgcat caccagtcgc caagttgata ccgtaacagt gacggcagac accgtgacga
     5281 gtgttacatg taaatacaga acggatagtc acttcttcca caccagcatt gacaatttca
     5341 cgcgccttgt cttctgtaat caattcattt ggaccaataa tcactgcacc agtttctgga
     5401 tgtttaacag ttttcttagt gtaacgaccg ttgagacgct cttcgagaga ctcgatcatc
     5461 tcttttcctt ctgcgataga acggatcaag agaccacggt cagttccaca gtcgtcctca
     5521 cggatgataa cgtcttgggc aacgtcgacc aaacgacgag tcaagtaacc tgagtcggct
     5581 gtcttaaggg ccgtatcggt cataccttta cgagcaccgt gagttgagaa gaacatttcc
     5641 aataccgaca aaccttcgcg gaagtttgaa aggattggca attccatgat acgtccattc
     5701 ggagcagcca tcagaccacg cataccggca agctgtgaga agtttgagat gttaccacgg
     5761 gctccagagt ccatcatcat aacgattggg ttcttaggat cttggttagc aatcaagcgt
     5821 ttctcaagtt tttcacgggc agcacgccat tcagctgtaa cagcattgta acgctcgtcg
     5881 tctgtgatca taccacgacg gaattgtttg gtgatttgtt cgacacgttt gtgtgattct
     5941 tcaatgattt cagccttgtc atcaacgact gggatatcgg caatacccac tgtcaatcct
     6001 gcaagagttg agtggtggta accgaggttc ttcatgcggt caagtagggc agaagtttct
     6061 gtcgtacgga aacgtttgaa gatttcagcg atgatatttc caaggttttt cttcttgaat
     6121 ggagggttga gctcaagatt gctgatagct tccttgatat ctccaccaag tggcaagaag
     6181 tatttagctg gaacaccttc tgtcaagttg gcattgtttg gttcttgcaa gtatggtagc
     6241 ccctctggca tgatatcgtt gaagagaatt ttaccaactg ttgtaagcaa gaccttatgt
     6301 ctttgctctt ctgtccaagg cttgttgagg ctgtctgttg cgataccaac acgtgagtgg
     6361 aggtgaacat aaccattgcg gtaagccata accgcttcgt cacggtcttt gaagaccatt
     6421 ccttcacctt cgcgaccagc ttcttccatg gtcaagtagt agttacccaa aaccatgtcc
     6481 tgagatggag taactaccgg tttcccatct ttcgggttca agatgtgctc agcagctagc
     6541 atgaggatac gagcttctgc ttgtgcttct tctgaaagtg gtacgtggat ggccatttgg
     6601 tccccgtcaa agtcagcatt gtaggcttca cagacaagtg ggtgcaagcg aagagcctta
     6661 ccatcaatca agactggctc gaaggcttgg atacccaaac ggtgaagggt cggtgcgcgg
     6721 ttcaaaagca ctgggtgttc tttaatcact tcttcaagga tatcccagat acgctcatct
     6781 ccgcgttcca ccaagcgttt agctgctttg acgttttgca cgatatcacg ggcaacgatt
     6841 tcacgcatga caaatggttt aaagagttca atcgccattt cacgcggcac accacattgg
     6901 tacatcttaa gagttggacc aacggcgata actgaacgtc ctgagaagtc aacacgttta
     6961 ccgagcaagt tttgacggaa gcgtccttgt ttacctttaa gcatgtggct caatgatttc
     7021 aatggacggc tacctggtcc tgtgattgga cgaccacgac gaccattgtc aatcaaagcg
     7081 tcaactgctt cttgaagcat acgcttctca ttttgaacga tgatacctgg tgcatttaac
     7141 tcaagcaaac gagccaaacg gttgttacgg ttgataacac ggcggtaaag gtcattcaag
     7201 tcagatgagg caaaacggcc accatccaac tgcaacattg gacgaagatc tggtgggata
     7261 accggaagga tgttaagaat catccattca ggtttgtttc cagacttgta aaaggcatcc
     7321 aaaacatcca aacgacggat ggctttgaca cgtttttgtc cagtagcagt tttcaattct
     7381 tctttgagtt cagcaatttc tttttcaaga tctacttgct tcaaaaggtc ttggatggct
     7441 tccgcaccca tcttagcaac aaatgaacca taaccatatt cacgcaagcg ctctcggtat
     7501 tcgcgctctg tcatgataga cttgtgctca agtggtgtat ccttaggatc aatcaccaca
     7561 taagccgcaa agtagataac ttcctcgagg gcacgagggc tcatatcaag ggtcaagccc
     7621 atacggcttg gaatcccctt gaagtaccag atgtgagata caggagcttt caattcgata
     7681 tgtcccatac gctcacgacg aactttcgta cgcgttactt caaccccaca gcggtcacaa
     7741 acaattcctc tgtaacgaat gcgtttgtac ttaccacaag cacattccca gtcttttgta
     7801 ggaccaaaga tcacttcatc aaagagtcct tcacgttctg gtttcaaggt acggtaattg
     7861 attgtttcag gttttttgac ttctccataa gaccatgaac ggactttact tggagaagct
     7921 agggtgattt gcatactttt aaaacgattt acatcaacca ctatttcttc cctttctatt
     7981 ctaagtgaac tgcttattct tgttcagcag cttcttctgt tgcttccgct tttgttgctt
     8041 tctcagcttc ttcagcttca aaggctgctt tagcctcttg ggctgctttt tcgcgggctt
     8101 tttcaaggtc atctacgtgg atgacatctt cgtccattcc ttcatccaag tcgcgaagtt
     8161 ccacttcttg gtcatcttcg tctaggacac gcatgtcaag accaagagat tgcaattctt
     8221 tgacaagaac tcggaaggat tctggaacac ctggttttgg aattggtttg ccttttgtaa
     8281 tagcttcata ggctttcaaa cgtccgttga tatcgtcaga cttgtaagtc aagatttctt
     8341 gaaggacatt tgacgcaccg taggcttcaa gagcccaaac ctccatctca ccgaaacgtt
     8401 gtccaccaaa ctgagcttta cctccgagtg gttgttgggt aacagttgaa taaggtccga
     8461 ctgaacgcgc gtgcaattta tcgtcaacca tgtggtggag tttgatcatg tacatgactc
     8521 caacagaaac acggttatca aatggttcac ctgtacgtcc atcgtaaagg attgtcttgg
     8581 catcgctatc catacctgct tctttaacag ttgaccaaag atcttcagaa cttgctccat
     8641 caaagactgg tgtcgcaatg tgaataccaa gagtacgagc tgccatacca aggtgaagct
     8701 ccataacctg accgatattc atacgtgatg gcaccccaag tgggttcaac atgatgtcga
     8761 ctggagttcc gtctggaagg taaggcatgt cttctacagg aacgatacga gagacaaccc
     8821 ctttgtttcc gtgacgtccg gccattttat ctccgacctt aatcttacgt ttttgagcga
     8881 tgtaaacacg aaccaacatg ttaacacctg attgcaactc atctccattt acacgtgtaa
     8941 agatcttaac atcacgaacg acaccatcgg caccgtgtgg tacacgaaga gaagtatcac
     9001 gcacttcacg agacttgtct ccaaagatag cgtgcaagag acgttcttca gctgaaagat
     9061 ctttctcacc cttaggtgtt actttaccta caagaatatc accttcttta acctcagcac
     9121 caatacggat aatccccatt tcgtcaaggt ctttgagggc atcttcacca acgtttggaa
     9181 tttcgcgagt gatttcttca ggcccaagct ttgtatcgcg cgtttctgat tcgtattctt
     9241 caaggtgaac agatgtgtag acatcgtcct tcaccaagcg ttcgctcatg ataacggcat
     9301 cctcgaagtt gtaaccttcc caagtcatgt aggcaacgat tgggttttgt ccaagcgcca
     9361 tttctccatt ttccatagaa ggtccgtcag cgatgaaatc gcctttttca acgacatcac
     9421 caacttttac gagagtgcgt tggttgtaag cagtacctga gtttgaacga cggaattttt
     9481 ggatgtggta aacatccaat gaaccatctt cacgacgaac ttctaccttg tcagcatctg
     9541 cgtaagtaac tttaccatca tactgagcaa tcacagccgc accagaatcg tgggctgctt
     9601 ggtattccat accagtacca acgtaaggtg cctgaggatt aatcaatggc acagcctgac
     9661 gttgcatatt ggctcccatg agggcacggt tggagtcatc gttttccaag aaaggaatac
     9721 atgctgtcgc aacggcaact acctgttttg gtgaaacgtc catgtagtca acaatattag
     9781 ctggatactc ttggttgacc ccttggtgac gtcccatgac aatcttctca gcaaaggttc
     9841 catcttcatt cagacgagag ttagcctgag ctacagtata ttcatcttct tcatcagctg
     9901 tcaaccaaac aatttcgttc gtgacaacac ctgtttcacg gtcaacctta cggtatggtg
     9961 tttgaacaaa accatatttg ttcaagtgtc cgtaagatga caagttattg atcaaaccga
    10021 tgttaggtcc ttcaggtgtc tcgattggac acatacgacc atagtgagtg tagtgcacgt
    10081 cacgtacttc atatccggca cggtcacgag tcaaaccacc aggtcctaag gctgacaaac
    10141 ggcgtttgtg agacaactca gaaagcgggt tgtgttggtc catgaactgt gacaactgtg
    10201 atgaaccaaa gaattcttta actgcagctg ttacaggacg gatattgata atttgttgtg
    10261 gtgtcaagac ttcattgtcc tgaacagaca tacgttcacg gacattacgt tccatacgag
    10321 aaagtcccaa acgtacttgg ttggcaagca attcaccaac cgcacggata cgacgatttc
    10381 caaggtggtc gatatcatct acacggccaa gtccttcagc caagttgagg aagtagctca
    10441 tctcagcaag gatatctgca ggagtcaccg tacgaacctt gtcatctggg ttagcattac
    10501 caatgatcgt tacgacgcga tctggatcag ttggagcaac aaccttgaat ttttgaagaa
    10561 caacaggctc agtcacaacg gctgcatcgt ttgggatgta gacaatcttg ttcaagtcgc
    10621 catccaaatg gctttcaatg ctttcaatca cgctacgagt cataatcgta ccagcttcta
    10681 ccaagatttc tccagtttca gggtctacca atggctctgc aatggtttgg ttgagcaaac
    10741 gtgttttaac attgagtttt ttattgattt tgtaacgacc aactgctgcc aagtcataac
    10801 gacgtgggtc aaagaagcga gctacaagca agctacgtga gctttcagcc gtcttaggct
    10861 cacctggacg aaggcgttcg taaatttctt tcaaggcttc gtctgtacga gagtccattg
    10921 gattcttgtg gatatctttt tcaacagtgt tgcgaaccaa ttcgctgtca ccaaagatat
    10981 caaagatttc atcatcacct gagaaaccaa gagcacgaac caaggttgta aatggaatct
    11041 tacgagtacg gtcgatacga gtgtaggtga tatcttttga gtcgctttca agttccaacc
    11101 aagctccacg gttagggata acagttgaac catagcccac cttaccattt ttgtctactt
    11161 tgtcgttaaa gtaaacacct ggtgagcgga ccaactgaga aacgataata cgttcaccac
    11221 cattgatgat gaaagtaccc atttctgtca tgattgggaa atcaccaaag aaaacttctt
    11281 gggtcttgat ttcgcttgtt tctttattga tcaaacggaa ggttacaaaa attggtgctg
    11341 agtagctagc atcgtggata cgagcttctt ctagcgtata ttttggttcc ttgatttcat
    11401 atccaacaaa ttccaactcc attgtgtctg tgaagtttga aattggcaat acatcttcaa
    11461 acacttcctt aagaccgtgg tctaggaaag ctttgaatga gtcagtttga atttcaatca
    11521 aatttggtaa gtcaagaact tctttgattc ttgaaaaact acgacgggta cgatgtttcc
    11581 cgtattgaac gtcatgtcct gccaagatga ttctcctttg taaataagtt ccaagccttg
    11641 tcaatcaggc ttttctaatc gtcatatggt tgtaaacccc ttatcaccgt gtcctcttga
    11701 cgaattttca gaatctttaa gcctctgtta caaatgctca aaatcttgaa aaaaagcaca
    11761 aaaagagcag ctaaatctga ctttttcaga agatttaact gctgtgagcc ttgtctggac
    11821 aatatttcag acaaaaccta cgacaaatga ttacccatat tataccctat ttagctagat
    11881 ttttcaaggg gtttcagtag gtttttggta aattttttcc catagaaaac ttggcatcac
    11941 attcgaatca cgctatggta caaaaaactg aaaaaactat tgactgaaaa tcattttcaa
    12001 ggtataataa taaacgttaa ggcggtatag ccaagtggta aggcacggct ctgcaaaagc
    12061 ttgatcgtcg gttcaaatcc gtctaccgcc ttctataact tgatttatca ggtttcaaat
    12121 gaacagaaag cccaatttga agggcttttt ttattttccc tcgaataaat acgtataact
    12181 ttaaaaactt ttggagcgag tttgtggcag agttctttcc atggcataat tcccttttga
    12241 aatcaattga aaaaagcttc gtcacgcctg gtgacaaaag cctctagttt attctgtttc
    12301 ttcttctatt tcgacttctg tgatttggac ttttaccttg gtaacacgtc cattcttcac
    12361 tttatcattg gttaagataa actgtttgtt ttggctgacc aattcatagc tgagtttctc
    12421 agtcgttgga atggttccca ctcctgtcaa ataataacca gcgatggtat caacgtcatc
    12481 actttccagt tcaacatcaa agtaattatt gaaatcattg agggtcatag ttccttgaac
    12541 aatgtaggta tcctcgccaa tctgatgaac atctattgct gctttgtccg tttcatcatc
    12601 aatttcacca acaatttcct ctaagaggtc ttccaaagtc accaaaccag ccataccacc
    12661 gtattcatcc agcaagattg ccatttgtct ttgggtattt cgcagttctt ttagcaagtc
    12721 atccacaaaa atagtttcag gtacaaaaag tggatcttgt aaaattctct tccaaacaat
    12781 attgtcaaaa ccgtccacaa agcctgcctt aaggagactc ttggtgtgaa tgattccaat
    12841 tacattgtcc ttatccccat cataaaccgg gatacgagaa taattttgtt ttaaaatact
    12901 ttggataatg gcttgactat catcctgaat atccaccata aaggcatccg ttcgaggaac
    12961 cataacctct cgtgccatca gttcatcgag cgaaaagaca ccttgtagca tctcaatctc
    13021 atcagcatcc aatgtttctt cactatttgt cagcatatag gcaatttcat cacgggtcat
    13081 cttttcatcc gcatcatcga atgacatagg agtcaaatgg ctcaagaaat tggtcgaagc
    13141 agctaaaagc caaacaaaag gactgactag ttttccgatc ccaatgataa tcggcgctgt
    13201 acgaattgcc aaggcatcct ttagattaag agcgattctc ttaggatata attccccaaa
    13261 aacgatggaa atataggtca aaaatgccaa ggatagaaaa gttgccacgg cttgtgctgt
    13321 ttcgccattc ccaagccaag aggcaatcac acgtcctaga gtatcagtta aactcgcccc
    13381 tgataagatt gtaatcaggg tgattcctac ctgaatggtt gataaaaagt ggttaggatt
    13441 ttctagtacc ttcagcaggc ggatgtagcg tctgtctcct tcttccgcct tttgttcaac
    13501 tcgggcacga ttaagagaaa ccatggccat ttctgtggct gagaaaaagg catttaatac
    13561 cgtcaagata aacaataaaa caaattgtag caacaaattc tgactacttg ggtcttccat
    13621 agttcttctc ctaaaatagt atcttatcct ttattatatc acaaaattta atccaataca
    13681 tattattttt ttcttagcgt aacttccatc tctctcccaa aactggaagt tagtctaaga
    13741 cggatttcca ctgatttcac gcgtttttct catactaaaa gctaggttgt gggaaactgg
    13801 aagttagttt tagaagttac tattttttat tagtacctgt tctagccctg cttaaactag
    13861 taaaaagaac tcctacatcc ataagtgaca taggagttta ggcttatctt ttactgagtt
    13921 acagttactt ctccagttcg gtaatccagt tgaagtccct tttgaacatt gggaactaga
    13981 acattgaatt ccaattctcc atccgaagac ttggcttcaa tctgtgaagc tgagggaact
    14041 aaatcctcgt ttgaagcgta gtaaagggtt acacggtaac ggacacgctt gttttggtcc
    14101 aaggctttac gcaccttgct ttcatagtag ttttgaccag tcgaatactc ggcttgtgcc
    14161 tgatttgccc aggctgtctg aacagcaatg tttttaggat tgcttgttga ggcatcaaaa
    14221 ccatccaaac caccgattaa ggcatagcct aacaaatgac ctctatcgac tgcatgggta
    14281 taagagccct ttagattctt gacctgatgc caacctggag gagtccaaga agttgaacca
    14341 ttcccagttt ctttacgatt cttgtactga cgagtggcct tagacaagag ggcattagct
    14401 acggttggaa cagtttcctt gcccactgtc tttgttttat tgtcagcgta gggcttactt
    14461 gaaaccttgg catctagatt tgttttatta ccattgacga taaaagcacc tgagccattc
    14521 cactccagac tcccctttat ttgactcttg actgcgtctg ttaagacact ctctgccaat
    14581 gcttgactag gagcttcagg cgcttgtttt ttctgactaa gcttggtttt aggactatta
    14641 gctgtcgact gcatctgctt gatataatag ctccctgtag acaaaagcaa taacactagc
    14701 agtccgatta gtgtctgtct tgtttttttg ttcatatttc tccttatctt tagaaaaagg
    14761 ctggttctcc agcctaattt cctgtaaatt tatgaatcaa ttcctgccat tttgctggag
    14821 acaggatagc ccatggatct tgacccttgc ccaagattcc ataacctacc attaaaccga
    14881 ttcctagagc cagagtacct aaaatcagta ctatgatgac taaaagtaaa cgcttgacta
    14941 cgtagcttga tttcttattc atcttcatca cttgcctcaa tccgctgaat cttagcacct
    15001 agctgcgcca acttctcatg gaaaccgtag taacctctat ccaagtgaac caatttaccg
    15061 accacagttt ctccctgtgc taccaaacct gtcaaaatca aagctgcact ggcacgaagg
    15121 tcagttgaaa gaacttctgc tccctgcaaa ggctgtccac caacaatacg agctgtatca
    15181 cggataatct cagaatgcaa gcccatgcgg cgcatctctt ctaggtgttg gaaacgattt
    15241 tcaaaaactg tctccaccat ggttgattcg ccttttgcaa ctgtcatcaa ggctgtaaat
    15301 tgagcctgca tatctgttgg aaatcctggg tggggcaagg ttttcacatg aacagctttt
    15361 agattttcca gttgagaacg aacacgaatt ccttcgtctt cttcaattac ttcaacaccc
    15421 atttcaagta acttggcaat caagggacgg ttgtgctccc agacagcgtc tcgaatcaag
    15481 acatcaccac cagtcatggc agcagctacc ataaaggttc ctgcttcgat acggtcctgg
    15541 actacattgt gagtcgtacc atgaagtttc tcaacaccag taatggttat agtctctgta
    15601 ccagcacctt tgaccttggc tcccatttca ttaaggagaa tggctaagtc aacaatctca
    15661 ggctcacgcg cagcattctc aatcactgtc accccatcag ccagagtcgc tgccatcatc
    15721 aagttctgcg ttgcaccaac acttggaaag tccatataga tatgagcacc atgcaagcgt
    15781 tctgccttgg cttcgatgta accagctgtc tgactaatct taacccccat agcttccaga
    15841 cctttcaaat gaagatcaat aggacggcta ccaatcgtac aaccacctgg catggatacc
    15901 ttggcatgac ccacacgggc aaggattggc cctaatacaa cgatggaggc gcgcatctta
    15961 ctgacatact tgtaaggggc ttcctcagtg atgtcgccag tagcatccac cttgacaaga
    16021 tgagcttcct catcaaagtc aaccttggca ttcaaaccac caactacctg attcataata
    16081 aagacatccg acaaaatcgg aacattctgc aagacggtct ttccttcact tgctagaata
    16141 gtcgctgcca acaagggtaa gactgcattt tttgctccct cgatcgtcac gcttcctacc
    16201 agacgattat cgccaccttg aaccacaatt ttatccatac tcgtttcctt tactcttgat
    16261 tttataataa tcctctaagc gcttcttgcc acaaatgata caggctaata aagaaagaac
    16321 tcaggatgta gcctatgaca atgctaaaaa aggctactaa taaacgaact tttcttgtat
    16381 tatcatctgt cacttttaaa accttctccc atctaacaag atcctttaaa aggtaaaaac
    16441 tcacataaat aaagaacata tgactactta gagtgaataa taattgaacc atttgactat
    16501 tataccatct tctctgtttg tgcgctagtt tggaaacttc actcaaaaat tagggattgt
    16561 ttttcaagta atcaatcgca tcttgtaggg ttttaacggg cacgattttc atatccgtct
    16621 tgattgtttt agccgcttct agggctgttt ggtagttgtt tttcgcgtcc ggatgcgcct
    16681 tttgttcttc ttcgctaaca gggttatcag gagcaaagaa aatagcagca cctgccctag
    16741 ccgaagctac aaccttctta tcaatacctc caatgtctcc cacattacca tcgcggtcaa
    16801 tggtacctgt accggcaaca atacgaccat tacgaagatc tgggtgagct atttgagtat
    16861 agatagctag actaaacatg agaccagcac ttggaccgcc aataccagct gttgaaaagc
    16921 taattgggac attgctgatt acctctgtac ggtcaatcaa gccgatccca attccatttt
    16981 tgccattttc caaggtgatg atttttcctt ctgcagactt ggtttgccca tcctcttcat
    17041 aggtgacctt gacggaatcc cctaattttt gagaactgac gtaatcaatc aagtctttgg
    17101 aactatcaaa ggtctgatca ttgactgctg tgaccgtatc agagatattg agaatccctt
    17161 taaaggttga attatccgtc acattcaaaa cataaactcc aaagtacttg agttcgatat
    17221 ccttaccagc tgtttttagt ccttgatact tggccatatt ttgcgatgtt tgcatgtaga
    17281 attgattgat tcgcataaat tcaacatcgg aagaaccacc tgtagtctcc tgagcactac
    17341 gaatatctgt aaaaggtgtc aaccaagcat aaatcatatg agctaaagtg gcatgttgaa
    17401 caccaaccgt aacgaattga taggcaccag cttccttatc ttctgtgtca tttactttaa
    17461 ggacttggcg aatatcttcc gaaccacctg gaacctctat ataataaggc aagggcacta
    17521 caaatgccaa gaaagtcaca atcaaggccg caatgacgta taagggccat ctaatctttt
    17581 ttttcatttc ttatttcctc caaaatactc tcgggaacat agcaggcaat atcctgacca
    17641 aacttcaaaa gctctctaac gcctgatgaa ctgatataga gatgttcagg tcgactatgt
    17701 aaataaatag tctctatatc agaagacagc tgatgattgt agtaatcaaa actggcttca
    17761 tattgcaaat ccgacgcatt cctcaaacca cgcactagac aagtagcacc caatcttttt
    17821 gcaacatcga ccaccaattc atcatgagaa gccacgactt caacattttc caaatgtccc
    17881 aaagcctttt ctagcccccg tttacgattt tcaagaggga gaaatccttg tttgtgggga
    17941 ttaaaaaaaa tacccacaca aagcttatca aaaagtctgc tcgcccgttc aatgatatcc
    18001 agatgcccat ttgtcatcgg atcaaatgag cctgtgaata agccaatctt atctgacata
    18061 gactgtcacc ttactaattc cataaatctt ttccttccag atacccagac aggcaatttc
    18121 ttctggaagt tcaacggcct tatccgtctc gcacacaacc ataacatctt cagaaaaaag
    18181 ctctctctca gccatttttt caatatctgc tacgatttgt tccttggcat agggagggtc
    18241 taagaaaacg aggtcaaatt ccccagatac ctgttccaat gccctctctg catccatctt
    18301 gaggagttga aattttccaa cttccttggt catctggata ttttcagcca cgatggtctg
    18361 agccttacgg tctcgctcca ccaaaacagc actggacatg ccacgcgata ctgcttcgat
    18421 agataaacca ccactacctg cataaaggtc caagactcgt cccccttcaa agtagggacc
    18481 aatcatgtta aaaatggctc ccctaacctt atccgaagta ggtcttgttg tcttgccttc
    18541 tagtgtcttg aggggacgtc ccccatagat tcctgatacg attttcatac tgtttattat
    18601 accaaattat agacaaaaag agaaagaaaa ccgaaccttg cggttcgatt ctctacaaaa
    18661 tattttcgta agtatcgcgg acttcttgag gccaaacact tgtttgcact tctccaatgt
    18721 gtctcttgcg aagtaggaac atggccatac gagattgtcc aattcctcca ccgattgtca
    18781 atgggaatag gccattcaac aaagacttgt gccattccaa ttccaagcgg tcttcatcac
    18841 ctgtaatttc cacctgacgt ctaagagttt cttcatctac acgaattccc atagaagaca
    18901 actcaaaggc tccacctaaa gactcattcc agacaagaat atcaccattt agacccttgt
    18961 agccattctc agactcgctt gtccagtcat catagtctgg tgcacgtcca tcgtgcggtt
    19021 taccatctgg caactcgcca ccgataccaa tcaaaaagac ggctccaaat tctttacaaa
    19081 tcgcattttc acgttctttc ggtgtcaagt ctgggtagcg ttctaccaat tcttctgtat
    19141 ggataaaggt gatttgtttt ggcaagatag actcgatgtc atagcgggct tcaacagcta
    19201 gctcagtcag gcgaatagcc ttataaatct tctcaactgt ttcttttaga taaacgatgt
    19261 tacgcttacc atttgggata accttctccc agtcccactg gtcaacataa acagagtggg
    19321 ttgcatccaa ggaatcctca tctggacgaa gggctttcat gtggacaaag agaccctctc
    19381 cttcaccaaa gccaaaacga gccaaggtgt ggcgtttcca tttagcaagt gagtgcacca
    19441 cttcataagt agcatcaggg atttggagaa ccttgaccga tactggattt tccacaccag
    19501 acaggttgtc ctgcattccg tcaccgacct tactcaagat aggaccttga acttcgacaa
    19561 cttctagctt atctttcaaa tactgggtaa aagtgttttt gacaaaggaa atttcttctt
    19621 gttgatggat aaaacttttc ttcatagaca actcctcaaa aatttaataa acgtattata
    19681 cacctatttc caagaaaaga aaagagaaaa tttaaaaaaa atcagtgtca ctcaaaacac
    19741 tgatttcata gaccttttag tcattgcgac caaagatacg tagaatactt aggaaaagat
    19801 tgataaaatc aagatagatg ctgagagcca ttgacaccac ccagcctgtc gctacacgac
    19861 cttgtgattg ttcataggcg aggcgaatct tttggttatc ccaagcaatc aaccctgaaa
    19921 aaaccaaaac catggctacg ctaatcatat aatcaaagaa gccactagcc aagaaaatat
    19981 tgactaccat agcaatcagt aaaccaatga gagctgccat cattgctcga ccaatcccac
    20041 tcaagtcttt cttggtaaaa ataccaactg ccgccatgac aaagaagaga agggcgctcg
    20101 atacaaaggc agataaaact gtacccggag tatagaaagc caccacaaaa ctaagggtaa
    20161 agccgtttaa aacggagtaa agtaaaaata ctggaagagc agctggacta tttcttgaag
    20221 ccatgctact ggcaacgaaa accaaagcca actccgcaaa agtagcaatg gtcaaccaga
    20281 gacgcccctg catcaaaaag taaaccaact gagactgaaa gaccgtcaac ataaggcctg
    20341 ataccaaagc agatagtccg attcccagac caacaaaggc ataaacctta gcgtaaaatt
    20401 gattgagacc tgcacggtca tgtataatag tgtgattcat ccatttctcc ttttctgtga
    20461 acatctttcc ttgattataa caaaaaaagt taaaattagc ttaaatggaa gattaaaata
    20521 ccggctgcaa ccgcaacatt caaactctcg gcctgcccct tcatgctaat atggaccaac
    20581 tggtctgcac tttcagccat aaggggacta attccttgac cctcatttcc cataactagt
    20641 acaaaatttt ctatagaagg cagttctctg taatcaacag aatctttaga tagggttgtt
    20701 gctagcactg ggatagctac ctttttagtt tcgtcaagaa gcgcttgact agtcatccgg
    20761 taaatgggca gatggaaatg actaccttgc atggaacgta gggttttgag actgtagatg
    20821 tctgccgact tatctgaaac aatcactcct gtaaaacctg ctgcatccgc agttcgaatg
    20881 atagttccta cattaccagg atcttgcaca tcttccaaaa acaagaactt gccctgactc
    20941 aaatcagctt gtcctacttc ttctttttga accacggcaa caattccctg tggagtctga
    21001 gaatccgcca aatctagcaa aatatcctct gaaatccaga cagtttgcgg aaaagcagct
    21061 aactgatttc gataattttc tagggcaaag attttctcaa tcgtcactcc agcttggaca
    21121 gcttcttcaa acaagtgcca gccttcaatc aaataggcag acttgcggta ttttttttgg
    21181 tgtaatttct tggcattttt taccacagaa ttggcttttg aggttataat agtcatagaa
    21241 atattataac acaatcaaag gggtttggta tgcaaaaggt tagaatgatt gcccaaggta
    21301 gggtacaggg agtcggcttt cgttggggtg tttacagctt ggcacttgaa attggtggca
    21361 tcacaggtcg agtatggaat aacgacgatg gcacagtgga aatcttagcc caagcagact
    21421 catctgctat catggcaaaa tttatccaag aaatccgaaa aggaccgaca cctttttcaa
    21481 aagtaagcta cttagatgtc aaactaagca actttcctcc ctactctgat tttaaaatcg
    21541 caaattaggt ctccagaact attgtatatt ttgtaaaaaa acagtagaat agaaaggtat
    21601 aatttttaaa gaaggaataa aaacagtgaa atctattaaa cgttttgcat tctcggctat
    21661 gggagtggct atgttgcttg tcttgactgg ctgtgtcaat gtcgataaaa ccacaggtca
    21721 gccaacagga tttatttgga atacgatcgg agcgcctatg gctgaagcca tcaagtactt
    21781 cgctactgat aaaggtctag gctttggtgt cgctatcatt atcgtaacca ttatcgtacg
    21841 cttgattatc ttaccacttg gtatctacca atcatggaag gcaacgcttc actctgaaaa
    21901 gatgaacgct ctcaagcatg tccttgagcc acaccaaaca cgtctcaaag aagcaactac
    21961 tcaagaagaa aaactcgaag cccaacaagc cctctttgcc gctcaaaaag agcacggcat
    22021 cagcatgttt ggtggtgtag gatgtttccc tatcctcctt caaatgcctt tcttctctgc
    22081 tatctacttt gctgcccaac atactgaagg ggttgctcaa gcaagctacc taggcattcc
    22141 tctaggttct ccaagtatga ttttggttgc ctgtgctggt gtcctttact atcttcaatc
    22201 gctcctttca cttcacggag tagaagatga aatgcaaaga gaacaaatca agaaaatgat
    22261 ttacatgagc ccactcatga tcgtcgtctt ctccctcttc tcaccagcta gtgtcacact
    22321 ttactgggtt gtcggtggtt tcatgatgat tctccaacag tttatcgtca actatatcgt
    22381 tcgtccaaaa cttcgcaaaa aagtccgtga agaactagcc aagaacccac caaaagcaag
    22441 tgctttctct aaaccaagtg gacgaaaaga cgttacccct gaacaaccaa ctgctatcac
    22501 aagcaagaaa aaacacaaaa atcgcaacgc tggaaaacaa cgttcgagat aagaagaaaa
    22561 aggcttagct gatttgctaa gtctttttgc aaatcaaaag cccgatgttt ccatcaggct
    22621 tcttttctat ccatgtacac gtttcatata atcttgataa ctttcggtat ccatgagttt
    22681 tttagcgttc ttgacgcgat ctgctgttgg tggtttgact ccttcgaggg aatatggaat
    22741 tccaagttca cgccacttga actcacccat ggtgtgataa ggtagaattt caaacttatc
    22801 aacatttttg agggtcttga cgaacttacc aagttcaatc aagtcatcat ctctgtctgt
    22861 caatcctgga actagcacgt ggcgaatcca gacaggtttt ccaatatctg atagatactg
    22921 ggcacaagcc aagatatttt tattagtttg tctagtgaca atcttgtgct gttcttcgtt
    22981 gatttccttg atatccaaaa gaaccaagtc agtgacagcc atgagtttgt caaacttctc
    23041 aaggtaacgt ggtttattac ggaaaggaag agcacaggta tccaaggtac agtggattcc
    23101 ttgttcctta gccttggtga agagagcaat caggaaatca atctgcaaga gagcttctcc
    23161 tccactgact gtaatcccac ccttatttcc ccagaaacca cggtagcgca aggcctctgt
    23221 caagacatca tctaccgtcc gttcacgtga cttattggac tccatagccc aagtgtctgg
    23281 gttgtggcaa tactggcaac gcatgtgaca gccctgcaaa aagacaataa agcgaatacc
    23341 aggcccatct actgacccaa agctttctgt cgaatgcacc attcctgtca cttgtccata
    23401 atcaattgtt tcttcagaca tatcgttacc ttccttgaaa acgttttata gttttattat
    23461 atcacgactt gaaggaaaaa caagattttt gcctattttt tagaaagcaa tctgtttttc
    23521 aatttcattt tcaatctatt atcagattat tcttcaaaat cgaagccata taaggttgca
    23581 aaatagtcct cagggcgctc tgcacggcgg atttgacggg ctttaccttc ttcggtatag
    23641 aggatttccg cagaacgtaa tttggcatta tactggtagc ccattgaaaa tccgtgggca
    23701 cctgtatcat gaattaccag caaatcaccg atttctgtat gaggcagttc gcgattgact
    23761 gcaaatttat cattgttttc acagagtgaa ccaaccacat ctaccgcttc agctggtcca
    23821 tctggatggg tcacgttgct aatatgatgg taagctccgt acatagctgg acgcatgagg
    23881 ttgactgctg aggcatccac acctagatag gtacggtagg tttccttctt atgagtcact
    23941 cttgtgacta gagcaccgtg aggtgccagc ataaaacgac ccaattcggt gaaaatcttg
    24001 acctgaccaa gacctgctga cgtaagaact tcttcataca ccttacgaac tccctcacca
    24061 atcaaggcga tatcgttcgg ctcctggtct ggacgataat taacaccaat accgccagaa
    24121 agattgataa agtctagcca aatgcccaac ttttccttga tttcaacagc cagttcaaag
    24181 agctgacgag ccaactctgg ataatagaga tgggtcacgg tattggacgc taggaaggag
    24241 tgaatcccaa aagtcttggc tcctttttcc ttcaagatag caaaggcttc aaagagctgg
    24301 tccttggtca tgccaaactt agcctcccca ggattgtcca taatgtctgt ccccagttca
    24361 aaaacgcctc caggattata acgacaagag atgatttctg gaatacctgc tgctctctcc
    24421 agatgttcaa tatcttcaaa ggcatccaag ttaatggtcg cacccaattc acgtgcatag
    24481 gcgtattcct tgtctggcgt gttgttggaa gagaacataa tctcagaacc cagaaagtcc
    24541 agtttatggc tcatcaaaag ctctacataa ctagagcagt ccacaccaca accttcttct
    24601 tggagaattt tcaaaatagc tggagttgga gtagccttaa ctgcaaaata ttccttaaag
    24661 cccttgttcc acgaaaaagc ttggttgacg gctcttgcct tctcacgaat ccccttctca
    24721 tcatacaagt gaaagggagt cgggaactcg gcaacaatcg cttctaactc ttctctattg
    24781 ataaatggtg ttttcataag cttccttcta ttctgtttgc aaagaaaggc tgggacaatc
    24841 gttccaacct ttagttctat ctcttattta tcttcgtcaa agatattgcc aacctcaaca
    24901 tcgatggctt ggttcttgac atcaatatta gttaccttca agagtgactt gtagtcaaac
    24961 tgcttttcac gttcttcttg ggcattgtcc gcaaagaccg ctacacctgc cagttctgag
    25021 tcgaactcgc gcaagagact aatcataccg ttgaccgttc cgccaccttt caagaagtca
    25081 tccacaatca agacacggct gcctgcctta agactacgtt ttgaaaggaa cattttctcg
    25141 atacggtcac cacttgaacc tgaaacatag ttgacgctaa cagttgaacc ttcggtaatt
    25201 ttcaggtcac ggcgcacaat gacaaaagag acattgagga cattggcaac tgcatttgca
    25261 agtggcacac ccttagttgc tacggtcata accgcgtcaa ttttttggtc cataaagctt
    25321 ttggcaataa tacgaccaat atttttcaag atggctggtg tgctaagcaa atcagacaga
    25381 tagatataac cacctggcaa gatacggtca ctttctgaca acttggtacg caagtcttca
    25441 accatttcct tagcatcctg actcgaaata gacggtgtga agatgacacc tccgccagcc
    25501 ccagtcactg tctggatatg accgatttca atttcctcaa aggcgcgttt gataatgacg
    25561 atatcttctg agatggatga tttagcagac tcatactttt cagcaaaagt attgagacta
    25621 gttagtttat aaggattatt aatcaaatag ttggaaatga caaccatccg atcacttctt
    25681 cttaatttca tttctatttc ctattccatt tttattcaaa aaatcaaaaa gcaaactaga
    25741 aagctggtcg ctggtggttc aaaacactgt tttgagattg tcgatagaac tgacaaaccc
    25801 tgtaatatac ctgcatatat acatacgaca aagcgatact accctagttt gaagagattt
    25861 tcgaagagta ttcatttttg tcttttactt attataccat attcacataa aaaaacgaac
    25921 attcttatcc taaaaaatgc tcatttttct taaattatca atctaaatct ggtttataga
    25981 aggaacgatt atccatagcg aagattttat tggacatctc tcctttatcc accaaagcca
    26041 gagctgttga catcatcatc atgcttgcat ccagattgtc aatcatatgg ataatctctg
    26101 cctccataat acgtggacgg actggacttc catattcaag caagccgtgg tgactgagga
    26161 tgacatgacg aagcaaaacg acttcttcct tggtatcatc gatgccgagt tccataactg
    26221 tcttggtaat ttcgctatca atgagagcga tatgtccaag aagattacct cgcactgtgt
    26281 actctgtctg gtctggcccc gtcaactcga taaccttagc taagtcatgc agcataatcc
    26341 ccgcatagag taagctctta ttgagctgag gataaacttc gctaatagcg tctgccaaac
    26401 gtaccatggt cgccgtatga taagccaagc ccgtttcaaa ggcatggtgg ttggtcttgg
    26461 cggctggata ggagtagaat tccttatcat acttggtgta gagatttcgg acaatccgtt
    26521 gccagacagg attttcaatt ttgaaaatca tttgcgacat gtagtcacga atttccttga
    26581 catcaactgg tgacttgacc ttgaaatcag ctgggtcatt gggttcacca gcttgaggca
    26641 ggcggagagt aatttgattg acttgagggg tattgttata aacttctcgg cgtcctttca
    26701 tgtggacaac cttacctgcg gtaaaggcct caatgttatg aggttgggca tcccagagct
    26761 tcccatcaat ctcgccacta tcatcttgga aggtaaaggc taggtagttt ttcccagctc
    26821 gagtttgcct caggtcagct gatttgatta ggtaaaagcc ttcaaataac tcatcttttt
    26881 tcatgtgact aatcttcata ttcttcctca ttttcttgaa aatagagtag atcaagcgca
    26941 ggctcacctt ctgacaactc aatgtgacgg agcgtccgct cgatagctat ggtacgacgg
    27001 tttaataatt catcaatatt gccagaggca tgttggagat gtttttgtgc cttgaccaga
    27061 ataccaccaa acttgccaaa ctcggtcttg acactggcaa gagtcttgct gatatggtcg
    27121 gcactctttt ggatattaag ggtcttgaaa ccaactgata gggagttaag aagggctgat
    27181 agggtacttg gtcctgcaac aataatctgt tcttcccgtc tcaaatcatc aaagaagacc
    27241 ggattgcgca caatctctga ataaagacct tctgtcggaa caaacaagat tccaaagttg
    27301 gttgtccgag gcggtgctat gtacttgctc ttgatatcct tggcaaagcg cttgacgctt
    27361 gctaggagtg acttacgaca gcgttcaatc tcatccttgt cacctgtctc ataggcttct
    27421 tccaagcggt aataatctgc cagtggaaac ttagagtcaa ttggtaggta gacgtattct
    27481 tggtcgcctt gtccgggtaa cttgatggca tactccactc gttcactaga gttttcaacc
    27541 gttgcgtatt ctcgttcgta ctgggcaggt gtcatgatgt cttcaataat ttgccccagt
    27601 tgcaattctc ccagaatccc tcgcgtcttg gttccagaga gaaccttgtt aagagctccg
    27661 acatcacggg caactgtctg catttctcca agaccacgat tgacagactc cagttgttta
    27721 gaaactgtct caaaggaagc ctgtaagcgt gtctgcaagg tcttttctag tttttcctcg
    27781 accgtctggc gcatttgttc caaacgttgc tcatttgatt cctgcaaggc ttggagacgt
    27841 tggtctgtct tgtctctagt ttggaggaga ttatctgtca tttcttgacg gacttgggtc
    27901 aggccttggt gcaattcaat ccgcacttct tgcaaacggt cgctgacagc cacttccaaa
    27961 tctttttggt ctaactggct ggcttgtctg gcttggtcaa agcggtaatc caactggtct
    28021 gacaaatgat ctgcctgatc ctccaagctc ttacttaagt gtttctcctg cctatcctgc
    28081 ctttgccaaa tcagaaaaag accagctagg ttggcaatta ataatagaat aagtaaactt
    28141 tccatcctac ctcctgtcct tgctatgcag tacgaccaca taaccatctg ggcaagttac
    28201 cgacacttcc ctatctatat attcgttaga agcgtacact tttttaaaga aaaaattttc
    28261 ctctgtcaac tcatacttgg ctccaagaat agtcagctgg ctatcccgaa ctggcataaa
    28321 ggctagatag tcgtagtctg aacgaggttc tagctgactg gtcccttctg gacaataagt
    28381 aatcaagttt tgcccatcct caatttctat ttgatgcata tagggtgcca acttaggatt
    28441 gctaggcaga aagacattgg ccaacatatg gtcaatacgg ccacccaagg caccgaaaat
    28501 agtaacctga gcctgaggat ttttctcaaa gatggttaag agagccaatt ccaaatcggt
    28561 atcatctttt tctggtcggg cttggacaaa atgctgggca cctttttgaa tcacctgtcg
    28621 ctcttcttcc gtcacagaat caaaatcccc aactgctaga gcaagaagca agtcttcttc
    28681 caagacccag agcgagcctc gatccacccc aacaaaagca tcaaaatctg tccgataatg
    28741 accgcggtct ccgcctgcaa aaacggcaac cctagtccag ttgttttctg agagtttgta
    28801 ctcgctcatt gacatctccc ttaaagacat aggaacctgc tacaaaaacg gtcgcaccag
    28861 cttctttggc ttgggcaata gtttggtcat caatcccacc atccacttcg atttcaaagt
    28921 tcaaaccttt ttcctcacga agagcaacca actcacgaac cttatccatg gtttctggca
    28981 gaaaggcttg cccaccaaaa cctggattaa ccgtcatgac taaaacttgg tcaactagat
    29041 gaaggacgtg cttgatggct tcaactgatg tgccaggatt gataacgact gaaggcttaa
    29101 ctccgagtga acgaattttt tggagggcgc catgaatatg aggcgttgct tctacatgga
    29161 tactgatgat gtctgcacct gcacgcgcaa aatcttccag atgatgctca gggtttgaca
    29221 ccatcaagtg gcaatcgaaa accatcttac tatgaggacg aaggctctcg accacacctg
    29281 caccaaaact gatttgcggt acaaaatgac tgtccatgat atcgatatgg gcatattctg
    29341 ccccagttgc ttctagacgt ttgatttcac gttcaaagtt ggcataatct gctgccagaa
    29401 ttgacggagc aatcttgtat tgagacatag gtttctcctt attttggaat ttttttgctg
    29461 acttttttat aggtttctct acgattttca atttcactaa ggaattgcag gtaattgtca
    29521 aaacggaagg ttgcaataac accctcttca acagctggtt tgacggcaca agacggctca
    29581 tgggtatggg tacaagtacg gaacttacaa tctcggctaa cagtagcaat ctctggaaag
    29641 gcctgattga ggtcttcagc ccttgatact tcatagtcca gagatgaaaa gcctggtgta
    29701 tctgcgattt tacccccgtt gagattgtaa aaactaacag ctcgagtggt atggcgcccg
    29761 cgacccagac tgtctgaaat ttctcccgtt tcaagattaa ggtctggtac gagtttattg
    29821 agaagagttg acttcccaac acctgtctgc cccataaaga ccgtaacctt gcctgttaac
    29881 aaagacagga gttccttttt actggtcaca aagtcatagc cgatgtcacc ataggtctgc
    29941 cggtaaaaat ccagttctcc cctatcttcc aacaaatcca ttttggaaat atagacaatg
    30001 ggatggatgc ccttgtgctc caaaagaacc aagaaacgat ccagcaaatt gctgttaaaa
    30061 tcaggttcct tgacggacat gattactaca gcttgatcga tattgacaat aggcggacga
    30121 accagactgt ttttccgttc gtgaattttt aggatatagc cttctgaatt ttcctcggca
    30181 gagaaatcta cccagtcccc aacataaggg gtatggcctt ttttacggaa attcccacgc
    30241 gcgcgtgttt gataaacctg gccatcactc tccacatagt agaaacctgc caaggcttta
    30301 atgatttgtc cctgcatctt agagtccttg ccctttaagt gcgtctgcta gaccggcaaa
    30361 ttctgccaag ctgagagctt ccccacgcac acttggtgac aagcctgcct ggtccaaagc
    30421 cttggtcagc ttgtccttga cctcttcagt cttaccaaag taacctgtca agttattcca
    30481 caaggtctta cggcgatggg taaaactagc cttggaaacc ttaaagaaga agttctcatc
    30541 ttccactgct acagctggct ctggacggcg caccattttc aagatggctg aatccacatt
    30601 tggcgctggc acaaagaccg tacgaggcac gataaaggca accttggctg tcatgtaata
    30661 ctgcacggcg atagacaagc taccgtaagc cttggtgtta ggctgggctg aaatgcggtc
    30721 cgctacttct ttctgcatca tgaccacaaa ctcactaaaa ggaatgccac tctcaatcaa
    30781 gtgcatgaga ataggcgtcg tgatgtagta aggcaaatta gccactacct tgattggcag
    30841 gtcaggattt ttaaaattct ggatatgttg cgccaaatca accttgagaa tatcttcgtt
    30901 aactacggtc acattatcaa aatcacgcag ggtatctgcc aaaattggca ccaaacggtg
    30961 gtcaatctca aaagtcatga cttgggctgc acgctcagcc agaaactccg tcaaggcacc
    31021 aatacctggc ccgatttcga tgacattgac ctggtcatca atttcagccg tatccacaat
    31081 tttttgaagg atattggtat ccgtcaagaa attttgcccg aaggactttt taaaggtaaa
    31141 accgtgacgc tccagcactg ccttggtcac gctataatct gcaattctca tctattctct
    31201 tttctattat ctgttactac tattgtaaca cattcccctt gtattttggg aatgaaaatc
    31261 ataccaaaaa actatctttc ctcatgaaga aagatagtct tggaaatctt taaagaggga
    31321 cttagcgctt ctctcgaaac atacgttcat actcttcgaa aatctcttca aaccacgtca
    31381 gcttcacctt gccgtactca agtacagcct gcggctagct tcctagtttg ctctttgatt
    31441 ttcattgagt atcaaaaaaa tcagcttctt gataacttct attttcgggt agggtcaaat
    31501 ccacatcctc ctccacatca tccactgtca gttcaaccgt ttgaggtaaa tttttcaaac
    31561 gataatgctt ttgcctttac ccttcaatac tagcaaaatc aagcaaatag ccatgcacta
    31621 ggatttgaac caaaaggaga tgattggtca tattttccat gatgaggata tcttctaaac
    31681 cacctctggc actaggataa tcagataccc tgcgaatacg gattttttca cacatgagca
    31741 agtttcctca tatcaatgat ttcatcagcc ttattccaga ctaggggatt atgggtcgca
    31801 atgatgataa ttcgattttc atctttcaaa tccaccaaga gattcataac ctcctctgaa
    31861 ttttcaggat caagagctgc tgttggttca tctgccaaaa tcaagggtgg atttttcaaa
    31921 atagtcttag caagggcaac tcgttgggcc tctcccccag ataaagtata gattttttgt
    31981 tctaaatcca aataccctag attaactttt tctaaagccc ccacttgcct ttccaaacgt
    32041 tctacttttg agattttctg accaacaaaa cccaaatcca aattttcttt gattgattgg
    32101 ttttctaaga ggccaaaatt ttgaaagaga tagcccatct gatctcgaaa atactcacga
    32161 gtgggaatgg tttttaaatc tttcccctga tagagaaccc ttcctccatc tatcttttct
    32221 agctttccca agatattcag cagcgtcgtc tttccgcttc cactctttcc gattaaggca
    32281 taaaccttgc ccttctccag cttgagattc aaaccagaga aaatcgcgcg gtcattaaat
    32341 ttcttttcca atccttgaat atcaatcatc ttattttcct ttcaatactg ccatgctacc
    32401 agcttcttct tttttgtcct gccttactaa aatcaagagg gcgttaagcg taaacaaagc
    32461 tacaactaga gcgctaatca aaccatctct tgtcaaaata ctagatagga ctaggccaag
    32521 caagagaact cctccttgcg ccagtaaata cttgccatga agctcataga ttgtcatacc
    32581 agcaagacgt ttaatcataa gctcccgtct gaactgctca aaatagagaa gattcatgga
    32641 atcaaataac aagatagccg tagatagggt caaaatcgtc ccaataagga gagagtaaaa
    32701 ctccacccgt ttgtcattta gtaccttggc aaaaaatagc tggcttttta ccaagtaaga
    32761 aaccttgtga tacagacctt gctcttttag agctgtgatg ctgtctccat atcgatctag
    32821 tttcaaggca ctattagccg tagttcccca caacatccct gcaacaggtc ttgttccagt
    32881 cgcttgcggc gttagaacta caataatcgg atcttttagg tactggcgtg ttgtcttgta
    32941 cccatcatta tagaggaaac gttcctgtcc tgtttctgta aaagctagcc ttacctgtgg
    33001 gaggtagtat ttctgactcg tcacttctac actttcagat gaaaagtttt gcaggtaatc
    33061 tgtaaacaat ccttggtagt aggtagactg ctctcgcaag ctctcaggca agatcagccc
    33121 aaactctccc tcagacaagt tttgcatctt gtccataaac tctgaagaaa cggtaatctt
    33181 ttcttctatc agatagcgtg gtgagacata gataacattc cctgacggtg tgtagtcact
    33241 gagacgattg ccatctaggt ccacttctgc tccatctgag aaattgtcaa cattgctcat
    33301 aatataaaaa gagtctgtat tggctaaccg ttcttcagta aatgtctgcc actcacgatt
    33361 atccttacgc gttccttctt catcggcaaa tgcactagac caaccaaagg atagacggta
    33421 acggtctgag gactggctcc atttattgct agctctctcc atttcctgca tttcacggta
    33481 gtggggtagg agagctgtcg cactcgatcc gaccaccaat acagctaaga gttgccccac
    33541 catcatcaat gtcatcatac gtttgagagg gagtttccct ttcaatagat ccaccagact
    33601 attttcctgt aaaccaagta gatagacgac acttagtaag gtagaaatcc ctgccagggt
    33661 caatccataa agtagaagag caatgatgac cagttgcacc gttgccataa acaaggcacc
    33721 ttgataccag agaatcccca atcccaaaag actggatacc agcactgagc agataagctg
    33781 tctcacatct tctaacactg gtctgagagc aactccaaac aagctctcac cagctattaa
    33841 gcgaatccct gcctgacgaa gggatttgat ccgataaatc agggtcagac tcataaaggt
    33901 cagcagaaaa atagctaaac tcagtagcac cataggggtg gccgtcaata gtaagactat
    33961 cgaaaatgga tcttctccat tcgaaacaaa gccttgatag ccaagttctt tcaaggtggt
    34021 ctgtaagctc actccatcca aacttcctga cacaatcaag taagacccta ataaatcact
    34081 cgtttctgca ctctccttgg aggaaatggt cagaccttct ggaagctctc cctcaccata
    34141 agtggcatag gtaaagagtg tttctccagc ttcatttggc tcaacgattc gacgggcgat
    34201 gagactatta tgctctaccg ccaagtgact caaagcctct gtcacctctt cgcgactagc
    34261 tactggttga tttatcatca ttcctgtcac atcaacagaa ggatagttac caaataaaat
    34321 ctcttctcga tggtcaacgg aagttatcac catataaagc gataccaata ccattgaaat
    34381 caaaataaat aaacgtttca tcatttcctc caaaaagtta cttggtcact tagtgagaag
    34441 gtgaccacag tcactgcgat gttaagaagc ttattaagtt ctttctgcta gcaacatctt
    34501 caaatatatt atatccgttt tttcaagaac tatcaaggac atcaatgtcc ttaaaatcta
    34561 actatttcat attattttta gcttatatct aaaaaaacag ttctaaagac gaactttaga
    34621 actgttttga tacgaggaca aggacatttt tgtcactgat tcactatgtt tataaatatt
    34681 ttttgacatc ctcttgccat tcctctgtca atttctttac tagaaattca tctccaatta
    34741 gctgcgcaaa tgttttagaa gactgataaa atgattcagc taaactaaaa tctctttcta
    34801 ctcttaatgc ttgcttccac aaaaacataa atacaagagg tttcttttga taatcttgag
    34861 ttttatccat aatttcttgt aaacacgata gataatcatt aaaacaatca gttactccta
    34921 ccttttcaag acaacacatt cctgaaataa gagtatctct aattataaag taattctcta
    34981 tttgagttac cttcttctgt tccaaaagaa agagcattag cttttcgatt tgatttataa
    35041 atttggcaag atcttttcta atgagcattt gataaaaaaa caaacgaatc aacaacatat
    35101 catttattga ataagcttct ttttctataa tctgaggcat atgttcctca agtattaact
    35161 ctgcatattc aggccttcta gttctagaag tatccaaaca cgcctgcaat acttcacatg
    35221 ctaattgttc ttctttagga agattatcaa agtatgtttt ataaatctct tccaaacacg
    35281 cttccttctt atcgtactcc tcttctttac catagattgg ttcacgtaaa atctggtatt
    35341 tcaattctaa ataagcagaa ggaagagctg aaaaatccgg cataaggcta taaactggaa
    35401 ctcctagccg gcgagcaata tagtctaact tgggcaaact cggttgcgaa gctccacttt
    35461 caattcgcga cagttgacga actgtcagtt cttgctcatc tccacaaaaa tctggacgac
    35521 taattccttt ttctatccga atctctttaa ttttttgacc aattaacatc agacgcctcc
    35581 tcttctacct gcttcgaaaa atactgtgaa acttaaaaaa ttgtaacggc tacatctttc
    35641 ctactcataa gatttcatag cttcttccac ttctgccaaa gtaaccccaa acaactctag
    35701 gcgtttgagg agttgcttgc cgttggaata gccgattcgg agagtctctc cgagatattc
    35761 tctacgctta cggctgtctg cccctgctag aaaaccaagg cgaatcaaat cgctacgact
    35821 aatgtcaaac tgactctcat gttcaaattg ttctgtcacc tgagctagag ccgttttcag
    35881 gtcttcatag ctggcatgct caattcccag agaacgcccc ttggtcttgg actttggaac
    35941 agcttcatct cgcttgagaa aggcatgccg aactgttgga atggccgtca taatcatgcg
    36001 ccgaatccgc tccccattaa aatctgggtc tgtaaagaca atgactccat gacgttggtg
    36061 caggcgctga atccgctcta tatcctgagc attgatggca gaacctcgag tctcataggt
    36121 ctccacatcg aaataacgtt tgagattgac cgtatcatca cgcccttcaa ccacgataac
    36181 ttgagaaatt ttctctttca ttacttgctg tccaatccaa aaattcgttc tgcatttgca
    36241 gtcgttgcta ccgccagctc ttctgtcgtc ataccacgca agtcagcgat aaagtcgacc
    36301 acatagcgag tataggctgt tttattttca cgaccacgct tgggtacagg tgctaagtaa
    36361 ggcgcatctg tttccaccaa aatcttgtcc aaaggtaact ctttagctgc ttcttggagg
    36421 tcagttgcct tcttaaaagt caccactcct gagaaggaaa tggtcatacc aagatccaca
    36481 aacttctctg cccactcaag cgtccctgaa aatgaatgca tgataccacc acgaggacca
    36541 acgccctcac tcttgataat ctcataggta tcttccagcg catcacgggt atggacaaca
    36601 aaaggcaaat ccaagtcctt agatagctga atctgacggc gaaaaacctg ctcctgcacc
    36661 tctttgggcg ctgtcatcca atggtaatct aagccaattt cacctaaagc cacaaccttg
    36721 ggatgtttta acttatccaa caagtaagcc tcaacttcct ctgtataagt accagcttct
    36781 gtaggatgcc aaccaatagt cgcatagagc tgatcatact catctaccaa ctccaaggca
    36841 cgctcaatcg tcggtttatc aaaaccaaca atattcatct gtgtcacacc catctcagca
    36901 gccaaggcaa tttcttctgc ctcacgacct gcaaattctt ctacattcaa gtgtgtatgt
    36961 gtatcaaaaa tcatctcttc taacctcgtt ttctatcttc tattatacca aaaaagagga
    37021 ggggcaccta atttttcggt ttcccctcct ctcttcaata gagagctatt ctgctatctt
    37081 ttctatccga tattgcccat ctcctattcc acagttagag acagaagaga ttggctacat
    37141 tgatagaata ttcctattca gctaaagtat gtgattatct tgggttctca cttttctcac
    37201 ccatcactag gattgatgac ttctcaaaat cccccttata atctaacttt aagtagagaa
    37261 tagaattttc tagctgttct attttctacg tctagtcttt cttcctagca aagcaaaacc
    37321 acttgttgcc accactagac caagactagc aagtgctgca cttgatgctg aacctgtgtt
    37381 aggtaattgt ttgccagcag gtgactggta ggtaacatct tgcattgttc ctgcagttgc
    37441 ttgcggtgca cttgttgtct taggagccac tggtctctta ggaactgtaa ccatgacaat
    37501 ggcttgcact ccaatcgttt ctgcttcctt cacatcttcc atagttttcg cattttcaat
    37561 ctctttgaga gctgcttgtt tttctgcttc cactcttgct aaaagttctg ctttttcttt
    37621 atcagaaagc ggtgcgcctt tgatttcatc ctgcttgtcc ttggctgctt tttcaatggc
    37681 atttttagct gattcttttt cagtagctaa ttgcttagaa gcttgtgatt gagctgagtt
    37741 ttgtgaacct gtgtgagctg atccacccgc ctggctacca ccgcctgcat tcgcattagc
    37801 atctgagctt ccagtattgc ctcccttatc tccacctggt gtattttcta gcttatactc
    37861 tggtgtagct tcttgagtta cagattcacc tttcgcagat tgttgaactg tatcttttcc
    37921 tagaatcgtc actactgaac catctgggaa tgtgattgtt gctgtaccat ctccagctac
    37981 attgattgtc gctccgtcta atgctgaacc atttgcttgt aaaatagcaa ccttaacttt
    38041 ttcttcatct gttaaatgag ttatatcttt tacatctact ctttgagcag gtgctttaac
    38101 agttgatttt tcagcagtcg gtttagtgct atcttgtgct tttggatctt gtgctaccaa
    38161 ttgatttcct ggaattgtaa tagctgaacc atctttaaag acgattgtcg ctgtgccatc
    38221 tccagctact tcaactttat agtaaaatga aataataaca ggacaaatcg atcaggacag
    38281 tcaaatcgat ttctaacaat gttttagaaa tagaggtgta ctattctagt ttcaatctac
    38341 tataacatcg cttccgaatc catttgcatc tactgaactt ttttacaaaa ataaaaatca
    38401 gtagactcga gttcctactg atttttgata gaattcgttt ggattatgca gccaaaactt
    38461 tgcgtccttt acgacgacga gctgccaata cgcgacgacc atttttagtt gacatacggt
    38521 tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg ttgataagta cgtttcacga
    38581 tgaatacctc ctcatagatt ttgtattcgt ttagccggct ataaagtgat cagttactaa
    38641 acatacttta ctattctatc tgattctttc acttttgtca atagctctag gcaaatgttt
    38701 ccagcttttt cgaatgcaag ccctatctac gatactgttt caagaaacga gtcatctttt
    38761 cttggatttg ggctaactca tcaacacgag gaaggtaaac gatacggaag tggtctggtt
    38821 cctgccagtt aaagcctcga ccatgaacca agagaacctt ttcctgcttc aagaaatcaa
    38881 ggacaaactg ctcatcatca tcgatacggt acatattgcg gtcgattttt gggaagatat
    38941 agagccccgc cttgggttta acggcagaca aacctggaat atcttgaatg gcattataga
    39001 tgaaatttct ttgctcgtag attcgtccac caggaagaag caattcatcg actgattggt
    39061 gaccccccaa ggaagtttgt acgacttgtt gggccaaaac gttagagcaa aggcgcatat
    39121 tggacagcat attgagccct tcgatatagc ccttaacatg agtcttaggg ccagacaaga
    39181 ccatccaacc cacacggaaa cctgctatgc ggtgggattt tgacagacca ttcatgctga
    39241 cacagaagac atctggtgcc aagctcgcca caggcgtatg cacatgtccg tccattacca
    39301 tgcggtcata gatttcatcc gcaaagataa tcaaatcatt ttgacgggca atctcaataa
    39361 tctccagcaa gagttcctta ggataaaggg ctccagttgg gttatttgga ttgataagga
    39421 caattgcctt ggtattggaa gtgatttttg acttgatatc gtcaatatct gggtaccatt
    39481 ccacagcttc atcacagata tagtgaacgg catttcctcc agctaggctg acagccgccg
    39541 tccatagagg atagtctggc attggcacta agacctcatc cccattgtcc aaaagcccct
    39601 gcatggacat gacaatcaac tcactgacac catttccaag gtaaatatca tcaatatcta
    39661 cgtttgggaa tttcttcagc tggcaatact gcatgatggc cttacgggct gagaaaatcc
    39721 ctttggagtc agaatagcct tcactatcac gcgcattcat aatcaagtca tgaatgacct
    39781 cgtctggcgc tgtaaagcca aattctgctg gatttcctgt attcagacgt aaaatctttt
    39841 ctccattcgc tcgcatccgc atggcttctt ccaatacagg accacggata tcataagcaa
    39901 catgctctaa cttactagac ttgttatatt ctttcatctt gtttcctcaa ttcatcagga
    39961 tagtaacatt ataccactag aaagagaatg cgacaagttt actgaaatgt gttactagtt
    40021 tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa cgtagagata gcaggtattt
    40081 tgcaaagaca catcacatac caaaagataa acctagaaaa agcaagccta gaaaggagtc
    40141 ataaagaaga ttaaacatga ggaaaagccc ccatatcatc aagtaatccc aacgcccact
    40201 tcgttttgca actagaaata agattagatg gtagagtaca tgaaagacta aaaatccaat
    40261 aaaacttgga taaagaagcg gtaccagact ctctaattgc cagatcatga tcacttctac
    40321 caagactgaa actaggataa ttccataata aaggaaattt gatttttgaa aagagttttt
    40381 catcattctc ccttccttca cttctttcct gctatccttt cttttatttt atcatatatt
    40441 tatatagatt ttaagcccta atattctttt gaaaaatact ttttttgctt tacttctata
    40501 gtgaaaaggt ttacaattat agtaagaaaa tttcaggagg tttcattatg gcacaacgtt
    40561 accaaaatat catggtcgca atcgatggtt ctaaggaagc ggacttggct tttgtcaagg
    40621 gagttcattc tgctctacga aacgacgcta aactcaccat cgcacatgtc attgacacac
    40681 gcgctctcca aagcgtatcc acctttgatg ctgaagttta cgaagaactc caagtcgacg
    40741 ctgaaagtct gatgaaagag tacgaaaaac gtgctaaaga tgctggggta gcagatgttc
    40801 atatcgtcat tgaaatggga aatccaaaga ccctgctagc acgtactatt ccagatgccg
    40861 aggaagtgga cctcatcctc gttggcgcaa ctggtctcaa cgcctttgaa cgcctcttgg
    40921 tcggctcttc atctgaatac atactccgcc atgctaaggt cgatttgctg gttgtaagag
    40981 aacaagaaaa aaccttataa tcacaaagaa aaggagcccc tagctccttt ttgtttacga
    41041 tttatttctc tctttatggc gttcgtaagc cttgagctgg cgctgcagtt cctttttaat
    41101 agcaggttct ggagcatatt tttcttccca attatctggt tttaagattt tatgggtcac
    41161 tggatcaaaa tgagccttac catctggaaa aattttcccc atattggcct gatggacaat
    41221 atcaaaaata cgttctgggt ccacccccat caagacaaaa ctgccgtagg tgaagtaaag
    41281 catgtcaatc aaggcatcca cttgccctat caaatcttgc tgagcaggtg tcttcttggc
    41341 tactttatct gctgccttat caagggcctg atgaagttgc gatacagctt gaccaaaatc
    41401 ttcttcagaa ggactggctg ctcgaacaaa ctccaccaat tcttctattt taaaaccagc
    41461 cctatgggtt gcaccctcta aatcccaagc tcgaggttct tcttgggttc gttcatccat
    41521 catgtggtgg aaggtcttga ccttattgaa atgatagtca cggctgacaa agactttttc
    41581 tgaagacaaa acaccaaaat gttccaaggc cttgtggatc ccatcttgtt gattgctagt
    41641 ggtaatgtgc ttggcagctt ccttgacact gctactacca tttcccatag cgaccgacat
    41701 accaacgcca gccagcattt caaggtcatt atctgagtca ccaaaagcca tgacttggtt
    41761 gaggtcaaag ccatattctt tcccaactcg gcgaatgcct tctaatttag aatttccctg
    41821 attgatgaca tccgatgcaa aaggattgct acgtgtcaat ttcaagtctt caaaatcagc
    41881 tgccgccttc tcagattctt ctggtgtcat cagcatcaaa acttggtaaa taggttgatt
    41941 catcaggtga agcaggtcct cttccttttg gggaacaacc ttgctgacca tgcgattaaa
    42001 agactgactc accgtccgag ttaaaacaga gggaacgaag cgactaattc gttgggaaaa
    42061 agaacccaga ccaaaggaca tgattttaga acccaacatg gcatccttgg tccctaaggc
    42121 aatctccgtg ccctcttttt tagcatagct aattagatgg cgcaaatgta acttggaaat
    42181 agggctcgtg aacaagactc tgtctttact aaagatatac tggccattat aggttaccgc
    42241 aaaatccaga tccaaatcgt ccatcaattc cttaacaaaa aaaggtcctc gccctgtcgc
    42301 tacgccaact agtacccctt gttctttgac aatcttaatc gcatccttag tggatttcaa
    42361 aacactcttg cgattgttga ccaaggttcc atcgatatca aaaaaaacag ctttgacttc
    42421 catcctatcc caatctcccc ttttgtgata caatgattat accacatttc agaaagagtg
    42481 agtaaatcat gcctaagaaa atccttgttt tacatacggg tggaactatt tccatgcagg
    42541 ccgatgcttc tggcgctgtt gtgacgagtt cagataatcc catgaaccat gtgtccaacc
    42601 cacttgaagg aatccaagtc cacgccttgg acttttttaa ccttccaagt ccccatatca
    42661 aacccaaaca tatgctggtc ctctaccaga aaattaaaga ggaagcagat aactacgatg
    42721 gagtggtgat cacacacgga accgatactt tagaggaaac agcctatttc cttgatacca
    42781 tggaagttcc ccatatgcct atcgttctaa caggagccat gcgtagctcc aatgaactcg
    42841 gtagtgatgg tgtttataat tacctaagtg ctttacgagt ggccagcgat gacagggctg
    42901 ctgacaaagg agttttggtc gttatgaacg atgaaatcca cgctgccaag tatgtcacca
    42961 aaacacatac gactaatgtc agcaccttcc agactccaac acatggcccc cttggtctca
    43021 tcatgaaaca ggaaatcctc tacttcaaaa cagctgaacc tcgtgttcgc tttgaccttg
    43081 atcacataca aggtttagtc cctatcatct cggcttatgc tggtatgaca gatgagctga
    43141 ttgatatgct ggatttagaa cacttggacg gtttgattat ccaagccttc ggagctggta
    43201 atattcccaa agaaacggct caaaaattag aaagccttct gcaaaaagga attccagtcg
    43261 ctctggtatc acgatgcttt aacggtattg ccgagcctgt ttatgcctac cagggtgggg
    43321 gcgtacagtt gcaaaaagca ggcgttttct ttgttaaaga actcaacgcc caaaaagctc
    43381 gcttgaaact cctcatcgcc ctcaatgccg gactaacagg acaggctttg aaagactata
    43441 tggaaggcta atactcttcg aaaatctctg caaaccacgt cacgtcgcct taccgtatgt
    43501 atggttactg acttcgtcag tttcatctac aacctcaaaa cagtgtgttg cgcagccttt
    43561 aaccagccgc ctagtccgct ctatggtatt cattaagtca acatctcttg tttaagagca
    43621 caaaatcagg aaatcttctc gattccctga ttttttctat ttacgttttc gtgttgagct
    43681 acgttctgtc aaaccatgag gtaagagaac ttcacgttct tccaactctt ccttatgcat
    43741 aatcttggtc aacatacgca tactaatggc accaaggtca taaagaggtt gggcaatcgt
    43801 tgtcaagttt ggacgggtaa agcgtgagat ttgtgaatca tcactagtaa taatttcaaa
    43861 atcttctggc acagaaacac ccttatcagc caaaccgttc aagactcctg ctgccaactc
    43921 atcacctgtc acaactgctg cagttgcatt tgatgaaatc aaacgctctg ctaaggcgta
    43981 accatcatca tagctatatt tagattcaaa taccaaaccc tcactataag tgattcctgc
    44041 ttttttcaag gtttccttgt agccaactaa acgaacctta ccattgatgt catccactag
    44101 cggaccgcta acgaaagcaa tacgctcatt ttctttagca aggtaactca ctgcatcaat
    44161 tgttgcttgc ttatagtcaa tattgacact tggcaactgg tgctcaacat cgacagttcc
    44221 tgcgagaaca atcggagtac gtgaacgcga aaattctgag cgaattttat ctgtcaagtg
    44281 ataccccata tagataatgc catctacctg ctttgaaaag agggtattga caacagaaac
    44341 ttctttctcg ttatcttcat cgctattagc taggacaata ttgtacttgt acatttctgc
    44401 aatatcatca atccccttag ccaaactcga aaaataacca ttggtaatat ttggaatcac
    44461 gacaccgaca gtggttgtct ttttacttgc aagaccacgc gcaactgcat ttggacgata
    44521 atccaaacga tcaattacct ctagcacttt tttacgggta ttctctttta catttttatt
    44581 gccattgacc acacggctga ccgtcgccat ggaaacacct gcttcacgag cgacatcata
    44641 aatggttact gtatcatctg cattcattcc ttttcctgtc ctttctatct cacacattct
    44701 tttacaagta gaggtactga ttgaagctct atatctactt acaaaagtga agatgtgaaa
    44761 atttcgtttt catatttcta cttattccat tctatcacta attgtaaaca ctttcaagtg
    44821 ttttttgaag attgattgaa aaaatttcat agaaaaccta ggtttagctc cttgctacca
    44881 ccttagacta aacaaaaagg aggaaactaa gccctcctaa agttatagta aaatgaaata
    44941 agaacaggat aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga
    45001 ggtgtactat tctagtttca atctactata ggtattgttc cattcactac cgtcaatttt
    45061 agcacatagt cttcatgaaa atattatatc atcataacca accagattct tttgcgatat
    45121 tagctgcctc tgttcgatta ccagcatcca gttttgaaag aatattggtg acatagtttc
    45181 gaatagttcc gtttgagagg taaagctgat ctgcgatttc ttggttggat aagccccggg
    45241 cgattccctt taagacagca atctcttgtt ctgtcaacgg attgggacgg gtcatcacca
    45301 tttccatcaa ttcaggcgaa tactccttgc gtccttcgag gacggtgtgc aaggtttgca
    45361 tgaggtctgc aatgcttcgt tcctttaata cataagcatc cactccagcc ttgaccgcac
    45421 gttcaaaata cccagcacgc ttgaaggtcg tcaccacaac cacctttgtt tcaagctttt
    45481 ctgatcgtat ccactccaag acttcaagac ctgtcttaac aggcatttct acgtcaagga
    45541 tggcgatatc tacagactcc ttttctaata gttggattgc ttcttgccca ttcttggctt
    45601 gaaggacaga ctccacatct ggttgaagcg tgagcaattg acacatggca tctcgcaaca
    45661 tactttgatc ttctgcgact aatactttca tcttagtttc tctccttata aggtagtcga
    45721 acttgcactt ccgttggatg tttctgactg attacactta cttctcctga aaatggaaaa
    45781 acacgatctc ggactgtatg gagctcatct ccttttagaa aagcaaagcc gcagccatca
    45841 tcacttactg ttaaaatgag ttccttctct gtccgttcta attttaagta agctttagac
    45901 gctttggcat gtttgatgat attggtcacc aactcaagca aaatcataga agccattgac
    45961 tccaattctt gagtaaggct agcagtatct agttggttat ccgtttccac ctcaattcca
    46021 gcaatttcta acatcttttt cacagtctct agttcggatg tcaaagttct agacttaaga
    46081 ttttccacga tagttcgaac ttcattcatg gaatccttgc tgatctggtg aatttctttt
    46141 aattcctttt ccacctgtgg ataagcctcc atctgaaata actgcaaggc tagatcagtt
    46201 ttaacactaa gcatagcaaa ggtatgcccc agactatcat gcaaatcttg accgatacga
    46261 ttgcgttcat tttcagcaag caatagattt atctgggcat tttgcttagc ctgtgcttct
    46321 ttcaaatcct ccacaattcg aatccgaacc aagccaaaag tcattaaatc gacaaaagca
    46381 agaattacaa gtagatagaa tagaaactca acttcgattc tctgaaaaat caacagttga
    46441 cccacaacaa ggacttgagc aagaagaaaa gtccagacat gtaaagactt taaaccacct
    46501 acgctgaaat gatagcttaa gagattggat aggaaaaaga aaaaccagat ataattaaca
    46561 gccacaaagg cagtattccc aactacataa gtcagcagga tgccccaata tagccaagat
    46621 agacgctggc tcttagttgt taaaacaccc aaatacgcca ctacaaatag aatatcaatc
    46681 aataaatgcc aggcagaaag ccacccagtc actacagtta ggatggggaa aaccataaaa
    46741 attaaactga tccaaaacat ataatgtatg cttttcagtc tttcaagcat taagcattct
    46801 ccttatgacc ttgaaggtaa atggtcaaac caaacaaaac tactgaaaaa acaagtaaat
    46861 aaactgtggc tgatagattg atgccaccct catttaagaa ggtcttgagc aactccatca
    46921 actgataggt cgggagacac ttacctacta cttgcatcca gtctggaaat aaagagatag
    46981 gcatccagag tccacctaaa acagccaacc ctagataaag aagattgccc acgacagaca
    47041 tcaactgact agttggtaag agagtcaagg tcaaaccaag cgctacgaag gcaatacttc
    47101 ctactatcag caaaagtgca gccccaatcc aatttccaag agacatgtcc acacctctta
    47161 caaaatgccc aactgagaaa accaccaaga ttgaaaccaa ataatcaacc agcatacttg
    47221 ttatctttga tagataatat tctaccatat ttacagggct atgacgcaat gttttctgcc
    47281 agttgttgat cttgtcggta tgtaaaacaa ctgggaatga gaagatagct gttgacatca
    47341 tggaaaatgc agtcatggag ataagataat cacgcataaa attcgcgagt tcacctggtg
    47401 tgtcctgata gataccagaa aaaaataaat agaaagccgt cggcatccct accgacaata
    47461 gataatagat taattgtcgt ttggtcaata aaaattctat cttactaagt gctatccatc
    47521 gtttcatctt agttatctcc cttctgcgtc tcttcaaaga ttgtattcaa caaactacga
    47581 ttattaactt cgatttcttg catcctacat cctgcttggg ctaatagttc ccagaaagca
    47641 tctgcttctc gagtgactac ttgtaaagaa tcttgcttta gggtccagtt ttcaaccaaa
    47701 tttgactgct ctacgacttc cttgtaagct ataggaagga taaagtgctt ttctatttcc
    47761 tcgctacgca tagctagagg cgtcgtatct cgaatcaact ctcccttatt taagagcaaa
    47821 atgcggtctg cagtgtgttc cacttcctca atataatggg acgaatagag aatggtgact
    47881 ccttgcgctt ttaactcctg gacaatttcc caaaaacgtt gacgagttga ggtatccatg
    47941 gacgcagtag gctcatctaa aaaaacaatc tttggtcgcc caattaaggt caagatgaaa
    48001 gaaaagagac gcttttgacc gcctgataat ttttctgcca actgttcttt ttgttgcttg
    48061 acaaactgca atagttgatc aatttcctga ttgctcaaag gatttggata gatacgttga
    48121 aagaaagcaa tcaactcttt aacttttaat ttctgaacga ttatattttc ttgagggagg
    48181 taagccctag tatagtctaa cttagaactc gtcactggta aaccttgaat agatacttga
    48241 ccgcttgtga ccagtttatc tccaagcaaa caatctaaga gtgttgtctt tccggcacca
    48301 tttggaccaa ttaaggcaac acattcacca tcgttgattt caaaggagat gttccgcaaa
    48361 atttccttgt cttttatttt cttactcaat ttttcaactt taaccacagt catgagactc
    48421 tcctttcaac cactccattc ccatagggaa aacgacgaaa atcataaatc caaaccccaa
    48481 agcaccacaa atgaattggc gaagcaaggc ttgatcaaac caatcagtaa acatttccac
    48541 taaccatacc aagagtgaca ggccgataaa gaaatagatg atccctctct tcattcctca
    48601 aactcctttt tcacatctct gactaatttc aaaccttctc taacaagcca agacatgatt
    48661 ccaaagccag caaagacctt ccaaggaaaa tggtagaaat cctcatccaa tcccgaaaac
    48721 atgagatagg tcataactcc tgctactact aaactcattg caataatcat tttatttctc
    48781 atctcttctt cccccatttc atactacaat tatagtcttt taaaatcaga ggaggcagaa
    48841 gcttctgtca ctagaaaata tgacaaatgt cataaaaatt ctgttcaaaa caagcaagat
    48901 acactataca ataaaacaca atcagaaaaa tctaaggcaa cttcctcaaa agagatatca
    48961 aacccaattc acaccataat gtaaactaat acttatttaa aatcaaaaag agtagaaatt
    49021 tttatcagac aaacacatat atagtgtatt gaatctataa cagtaggcct taaatactaa
    49081 aatatttcta taaattaatt taactttcc
//
LOCUS       DAVKHZ010000007        48042 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000007 DAVKHZ010000000
VERSION     DAVKHZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.007"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            179..1942
                     /gene="aspS"
                     /locus_tag="VWW99_000347"
     CDS             179..1942
                     /gene="aspS"
                     /locus_tag="VWW99_000347"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9538244.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1920..2861
                     /locus_tag="VWW99_000348"
     CDS             1920..2861
                     /locus_tag="VWW99_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9538245.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(3026..3106)
                     /locus_tag="VWW99_000349"
                     /pseudo
     CDS             complement(3026..3106)
                     /locus_tag="VWW99_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3242..4228)
                     /locus_tag="VWW99_000350"
     CDS             complement(3242..4228)
                     /locus_tag="VWW99_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9538246.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4238..5038)
                     /locus_tag="VWW99_000351"
     CDS             complement(4238..5038)
                     /locus_tag="VWW99_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9538247.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5291..6133)
                     /locus_tag="VWW99_000352"
     CDS             complement(5291..6133)
                     /locus_tag="VWW99_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9538248.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6135..7427)
                     /locus_tag="VWW99_000353"
     CDS             complement(6135..7427)
                     /locus_tag="VWW99_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9538249.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7538..8809)
                     /locus_tag="VWW99_000354"
     CDS             complement(7538..8809)
                     /locus_tag="VWW99_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9538250.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9359..10876
                     /gene="malQ"
                     /locus_tag="VWW99_000355"
     CDS             9359..10876
                     /gene="malQ"
                     /locus_tag="VWW99_000355"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9538251.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            10902..13160
                     /locus_tag="VWW99_000356"
     CDS             10902..13160
                     /locus_tag="VWW99_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEU9538252.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14205..14777)
                     /locus_tag="VWW99_000357"
     CDS             complement(14205..14777)
                     /locus_tag="VWW99_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538253.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(15400..16248)
                     /locus_tag="VWW99_000358"
     CDS             complement(15400..16248)
                     /locus_tag="VWW99_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9538254.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            16400..16678
                     /locus_tag="VWW99_000359"
     CDS             16400..16678
                     /locus_tag="VWW99_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538255.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            16680..18743
                     /locus_tag="VWW99_000360"
     CDS             16680..18743
                     /locus_tag="VWW99_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9538256.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYNKEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(18821..20077)
                     /locus_tag="VWW99_000361"
     CDS             complement(18821..20077)
                     /locus_tag="VWW99_000361"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9538257.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAIRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            20219..22684
                     /locus_tag="VWW99_000362"
     CDS             20219..22684
                     /locus_tag="VWW99_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9538258.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNVIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEEKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            22749..23660
                     /locus_tag="VWW99_000363"
     CDS             22749..23660
                     /locus_tag="VWW99_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9538259.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIVLIILPMIFLSIEEAKESR"
     gene            23890..24588
                     /gene="dapD"
                     /locus_tag="VWW99_000364"
     CDS             23890..24588
                     /gene="dapD"
                     /locus_tag="VWW99_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9538260.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            24656..25786
                     /locus_tag="VWW99_000365"
     CDS             24656..25786
                     /locus_tag="VWW99_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9538261.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            25798..26337
                     /locus_tag="VWW99_000366"
     CDS             25798..26337
                     /locus_tag="VWW99_000366"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9538262.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVAYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            26321..26998
                     /locus_tag="VWW99_000367"
     CDS             26321..26998
                     /locus_tag="VWW99_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9538263.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(27065..27964)
                     /gene="galU"
                     /locus_tag="VWW99_000368"
     CDS             complement(27065..27964)
                     /gene="galU"
                     /locus_tag="VWW99_000368"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9538264.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(27986..29002)
                     /locus_tag="VWW99_000369"
     CDS             complement(27986..29002)
                     /locus_tag="VWW99_000369"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9538265.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            29214..29654
                     /locus_tag="VWW99_000370"
     CDS             29214..29654
                     /locus_tag="VWW99_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9538266.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(29777..30193)
                     /locus_tag="VWW99_000371"
     CDS             complement(29777..30193)
                     /locus_tag="VWW99_000371"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538267.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(30261..30911)
                     /gene="phoU"
                     /locus_tag="VWW99_000372"
     CDS             complement(30261..30911)
                     /gene="phoU"
                     /locus_tag="VWW99_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9538268.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(30926..31678)
                     /gene="pstB"
                     /locus_tag="VWW99_000373"
     CDS             complement(30926..31678)
                     /gene="pstB"
                     /locus_tag="VWW99_000373"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9538269.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(31680..32495)
                     /gene="pstA"
                     /locus_tag="VWW99_000374"
     CDS             complement(31680..32495)
                     /gene="pstA"
                     /locus_tag="VWW99_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9538270.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(32488..33351)
                     /gene="pstC"
                     /locus_tag="VWW99_000375"
     CDS             complement(32488..33351)
                     /gene="pstC"
                     /locus_tag="VWW99_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9538271.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(33469..34344)
                     /locus_tag="VWW99_000376"
     CDS             complement(33469..34344)
                     /locus_tag="VWW99_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9538272.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(34511..35842)
                     /locus_tag="VWW99_000377"
     CDS             complement(34511..35842)
                     /locus_tag="VWW99_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9538273.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(35843..36550)
                     /locus_tag="VWW99_000378"
     CDS             complement(35843..36550)
                     /locus_tag="VWW99_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538274.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(36634..37110)
                     /locus_tag="VWW99_000379"
     CDS             complement(36634..37110)
                     /locus_tag="VWW99_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9538275.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            37117..37644
                     /locus_tag="VWW99_000380"
                     /pseudo
     CDS             37117..37644
                     /locus_tag="VWW99_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(37959..39650)
                     /locus_tag="VWW99_000381"
     CDS             complement(37959..39650)
                     /locus_tag="VWW99_000381"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9538276.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            39786..40232
                     /gene="argR"
                     /locus_tag="VWW99_000382"
     CDS             39786..40232
                     /gene="argR"
                     /locus_tag="VWW99_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9538277.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            40283..42817
                     /gene="mutS"
                     /locus_tag="VWW99_000383"
     CDS             40283..42817
                     /gene="mutS"
                     /locus_tag="VWW99_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9538278.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            42964..44658
                     /gene="patA"
                     /locus_tag="VWW99_000384"
     CDS             42964..44658
                     /gene="patA"
                     /locus_tag="VWW99_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9538279.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(44988..45251)
                     /locus_tag="VWW99_000385"
                     /pseudo
     CDS             complement(44988..45251)
                     /locus_tag="VWW99_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            45449..47215
                     /gene="patB"
                     /locus_tag="VWW99_000386"
     CDS             45449..47215
                     /gene="patB"
                     /locus_tag="VWW99_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9538280.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            47354..>48042
                     /locus_tag="VWW99_000387"
     CDS             47354..>48042
                     /locus_tag="VWW99_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9538281.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
BASE COUNT        13966 a         9157 c        10503 g        14416 t
ORIGIN      
        1 aaccatacct acggtaaggc gacgctgacg tggtttgaag agattttcga agagcattag
       61 cgctaatttt tagggctttc acctgacttt taaagactgg caaacttttc tgatgatttt
      121 catgaagagc ctgcgctttt atggtaaaat agtaacagaa taaaagagga gagaaataat
      181 gaaacgtagt atgtatgctg gtcgtgttcg tgaggaacac atcggacaag aaataacctt
      241 gaaaggatgg gttggccgtc gtcgtgacct tggtggtttg atctttatcg atcttcgtga
      301 ccgtgaagga atcatgcagt tggttatcaa ccctgaaaaa gtatctgcag aggttatggc
      361 aacagctgaa agccttcgta gcgaatttgt tattgaggtg actggtcagg tcgctgcgcg
      421 tgagcaagcc aatgataagt tgccaactgg tgcggttgag ttaaacgtga cagctcttat
      481 tgtgcttaac acagctaaga caacaccatt tgagattaag gatggcattg aggcaaatga
      541 cgatacacgt ttgcgttacc gttaccttga ccttcgtcgt ccagaaatgt tggaaaatct
      601 taaacttcgt gccaaggtga cccactctat ccgcaactac ttggatgagt tggagtttat
      661 cgacgtggag acaccattcc tttctaagtc aacgcctgaa ggggcgcgtg attatttagt
      721 gccgtctcgt gttaataagg ggcattttta cgctcttcct caaagtccac aaatcacgaa
      781 acagctcttg atgaatgctg gttttgaccg ttactaccaa atcgttaaat gtttccgtga
      841 cgaggacttg cgtggagacc gccagcctga atttactcag gtcgacttgg aaacgtcctt
      901 ccttactgag caagaaatcc aagatattac agaaggcttg atcgcgcgcg tgatgaagga
      961 aacaaaaggc atcgaagtaa cgctaccgtt ccctcgtatg aaatacgatg atgctatggc
     1021 tctttacggt tctgacaagc cagatactcg ttttgacatg ttgcttcagg acttgacaga
     1081 agtggtcaaa ggtgtagact ttaaagtctt ttcagaagca cttgctgtaa aagcgattgt
     1141 ggtcaaagga gctgcggaca actattcacg taaagacatc gacaagatga cggaagtagc
     1201 caaacagtat ggtgccaaag gtcttgcttg ggtcaaggtg gttgatggag aattaaacgg
     1261 accagttgcc aagttcttga ctggtatcca agaagaattg acaacagcgc ttgctcttga
     1321 agataaggac ttggttctct ttgtggcgga tacgcttgaa gtggctaatg caacactggg
     1381 tgcccttcgt ggacgtattg ctaaagagct tggcttgatt gataatgata agttcaactt
     1441 cctttgggtg gttgactggc cgatgtttga atggtctgaa gaagaaggcc gctacatgag
     1501 cgcccaccat ccattcacac ttccacagga agagactgct cacgaattag aaggtgattt
     1561 ggctaaggtt cgtgccattg cttacgatat cgtcttgaac ggttatgagc ttggtggtgg
     1621 tagccttcgt atcaaccaaa aagaccttca agaacgcatg ttcaaggctc ttggtttctc
     1681 agctgaagaa gccaatgacc agtttggttt ccttcttgaa gccatggact atggtttccc
     1741 accacacggt ggtttggcta tcgggcttga ccgttttgtc atgttgcttg ctggagaaga
     1801 aaatatccgt gaagtcattg cctttcctaa gaacaacaag gcaactgacc caatgacaca
     1861 agctccatca acagtcgctc tcaaacaact agaggaactc agcttacaag tagaagaaga
     1921 tgaaacaaac aaaacgaatt aagcggtggc gctattatct gcgccgcttt gcttatcaga
     1981 taaaaatttt acgtgtctta caaagtatct ctcgagaaaa gtatgatgag aagatttcgg
     2041 cctctctggt ctatggtttt ttatcagcag tagcagttaa tttctttttc caaccagggc
     2101 atgtgtattc gagtggtgca acaggtctgg cacagattat ctctgccttg agtaatcact
     2161 ggtttggttt tcatattccg atttcgctaa gcttctacgc cattaacttc cctttgatgg
     2221 tcttagcttg gtatcagatt ggccataagt tcaccgtctt tacctttatc acggtatcta
     2281 tgagttcctt ctttatccag tttgtccctg tggcaacctt gacggaggat cccattatca
     2341 attccctttt tgggggtgtt gttatgggtt tggggattgg ttttgctctt cgaaacaata
     2401 tctccagtgg tgggacggat atcgtcagcc tgactattcg caagaaaacg ggtaagaatg
     2461 tcggtagtat ttctttcttg gtaaatggaa ctatcatgct gatagcaggt ttgacctttg
     2521 gttggaaata cgctctttat tctatgatta ccatctttgt ctctagccgt gtgacagacg
     2581 cagtctttac taagcaaaag cgtatgcagg ccatgattgt gacaaatcat ccagagaagg
     2641 taattgaaaa aatccataaa aaattgcacc gcggagcaac catgatccac gatgcagaag
     2701 gaacctataa tcacgagaga aaggcagttt taatcactgt cattacacgt gcagagttta
     2761 atgaatttaa acagattatg acacaagtgg atccaagctc ctttgtctct gtctcggaaa
     2821 atgttcatat tctaggaaga tttgttgaga tagataatta gtgattaata ctcttcgaaa
     2881 atctcttcaa accacgtcag cttcaccttg ccgtatatat gttactgact tcgtcagttc
     2941 tatctgcaac ctcaaaacca tgttttgagc tgacttcgtc agttctatct gcaacctcaa
     3001 aaccatgttt tgagctgact tcgtcagttt catctacaac ctcaaaacag tgttttgagc
     3061 aacctgcggc tagcttccta gtttgcactt tgattttcat tgagtataaa aagatcttgg
     3121 gaaaagactc aatttcagta gaaaaggaag taaatcttcc cacaataaaa cgcatagtat
     3181 caagtttttt caatacctga tagtatgcgt ttttgctttt aacgactttt tgcccagtct
     3241 cttatttttc gataattttg tgggcgatca attgacggta acaaatttgt ttattgtttt
     3301 tattatcatt aataatctgg agaatagttt caagggaaac acgaccaagc tctaaactat
     3361 tgatatcgac ataggctgcc aagttgagct tgggattaac cgagtcaaag ctgagaacag
     3421 ggacatccag ctggtgtttg gcaatatagt tacaaacacc ttcagctagg aggctatcgg
     3481 ttgtgatgat agcatcaatt tgtggatcgt gcttgaataa tcgcttgcta aatttatagc
     3541 ccttttcttc cagaaactcg tcggcaaagt agatgcgatt gttgtcagtg gtaagtttgt
     3601 aatgtttaag cgcctgttca tagcctgtta aacggtcttt ggtcacgaag agctttttac
     3661 ttcctccgat aaaggcaatg cgtttgcagc cttttttgat gaaatattca gtcgcatcaa
     3721 aaccagcttg aacattgtcg ttgtcgacaa gtgggatgaa aggagataga gatttaccta
     3781 agataaggaa ggggaactgt tcttctgcga cgagttttac gagagggtct tcttcttggg
     3841 catagagaaa aattagccca tctacacgct tgccgtagac catttgtgaa atagcgttga
     3901 gacgctcctt ctcatctttc cctgttgcta tctgaatggc atagtggttt tcagatgcga
     3961 cttgagagat gccacgtaga accgatggaa agaaaggatt ctggtagaag gcgtctgagt
     4021 catcaggaag aactaatccg ataacctgag tatagctgct taccaagcta cgagcgttga
     4081 ggtttgggtg gtagttgagt tccttcatag ctttgcgaac gcgttttttt gtttcgtcgc
     4141 taatggttga tttattttga ataacacggg ttacggttga aggcgaaaca ccagcagcct
     4201 tggccacgtc tttaatcgta acgggcataa aaatctccta tttatggtaa tttggatcac
     4261 ggaaatgtgt tttataaaag atagtgacca gatacagaac aaaaagaata ttttgtacag
     4321 taatcagggt tgtcatattt tggccaaata atcccaaaat aagtgtaatc agagtcggca
     4381 atcctaaaca gttcaagata aagtgatagc actctttaaa ggttctaaat gaaaagaggc
     4441 gtgatttctt agtgatataa aggagaagac tagctcctag agagacgata aagaaattca
     4501 aaccaaagag gaagctcgca ccgagaacta ggaagaggct gatatagaca cgattttgtt
     4561 ggtaccagtc tttagaaatt gcttgggtca agctgtcttt gcttttgaaa ctctcagtct
     4621 gaatggctcg gtaagagatg cgggtcagtt ccttgctttc cttgctgatg actagctcat
     4681 ttgtatcgaa atgcagttgc aagtccttag gtaattcctt gatttgactt ggaccaatca
     4741 caacagaagg ggcttgacta gctgttccag tataagttaa tgtgccatcg acaattgtag
     4801 catgttcaga gagatcctgg acaaccttat ctgtcagagg ttcatagaca ttatcgataa
     4861 aagtttctag cggataggtc tcctgggagc tgttttggat agcaatgggt accatagaca
     4921 agctgataag gaagatactg gtaaagagaa gttgaaacca gttgagctcg aaacgtttgg
     4981 acaggggctt acgaaacccc caaatacttg aaaaatagga aaatggatat ggaagcatag
     5041 gtatctttct aaaaggtgtt ttctatatga gtattattat atagagaaat gcgctttatt
     5101 tcaagtctag gagaaaaatt gcttgctaca aggatattat ttacagtgac aattgctaat
     5161 aatcaatgaa aatcaaagtg caaactagcc acaggttgct caaagcacaa ctttgaggtt
     5221 ggagataaag ctgacgtggt ttgaagagat tttcgaagag tataaaatga aaaagggtgg
     5281 cggggataaa ttatcccttg tcgccaccac ttgtaagtcc tgaaacaaag ttcttttgta
     5341 ggaaaaagaa gagaatacag attggaaggg cgatgaggat agcacctgct gagaagtagg
     5401 caatcttcaa gtttttcgca ttgttaacga aggtttggag acctacggca acagtaaagt
     5461 attctttctc acgaagcaag aaactagaga ggatgtagtc cccgaaaggt cccatgaagg
     5521 cccagagagc ttgtacggca accattgggc gaacaagtgg tagaacaatt tgccagaagc
     5581 ggcggaagtg tcctgcaccg tctagttttg cagattcgtc taaagacatt ggcactgtat
     5641 cgaagtagcc tttcatgagc caagcattca tcgggatacc accaccaacg tagaggaaga
     5701 tgaggaacca gttgtggtta agggcgttca acataagcgc cataacgaag aaggctgtca
     5761 aagcggccat agttggcacc atttggatga tcaagaagaa gaccaaactt tgtttacgag
     5821 ccaagaagtt gtaacggctg taggcataac cagcaagtac gatgatactt gtttgaacag
     5881 ccatggtaat taaggcgata atcaaagtgt tgaggtacca agtaccgtac aaggtttcag
     5941 tgaagaggcc tttaaagtta tcaaaattga ggtcgatatt agtatctagt ttaaaggctg
     6001 agacgttacc tgctttaaag gctgacataa tggtaatcaa cagtggatag ataattacaa
     6061 ttgatagacc aatcaggtaa aggtaagtaa ggctttgagt cagtctacgt ttgagtttaa
     6121 ttgagttatt catcttagac gtcctccata tcaaatgcgt gtagtttctt gaatgcgatc
     6181 atagagattg agatgacaat gatagagata atcaaggtaa cagctgccgc cattgagtat
     6241 tgaggagatg tacctgttgt caaacggtag atccatgaga tcaagatatc ggttgaacca
     6301 gctccacctc cgacactacc aggtcctcca ccattgaaga ggtacatgat agagaagttg
     6361 ttaaagttga aggtgtattg gctaatcaaa gtaggtgccg caacagccaa aatcattggg
     6421 aaagtgatgt tgcggaattt ttgccaagcg ttggcaccgt caatataagc tgcttcgtaa
     6481 aggtcgttag gaatagattg caagataccc aaagtcaaaa cgtagatgta tgggaatccg
     6541 agccaacctt gcatcataat caaggcaatc ttagtccaag ttgggtctgt tttccaagga
     6601 ataagagctc catcaaggaa aggaaggaat ttagccaaga ttggcaatac ttgagtgttg
     6661 atagcaccga cactatcgtt aaacatgttt gagaatgtca agatagtgat gaaggctggg
     6721 acagcccaag gaagaaggaa aataacacca aagatacgtt ttcctttgat aaatggttgg
     6781 ttggcaatga tagctgtgaa gataccaatt acgatttgta aagtagaagc tgccaaagcc
     6841 caaatgatag tccaagaaag aacagaacca aaggcagaac ggaaggtact caagctccaa
     6901 atgtttgtaa agttggtcaa accaacccag tccaacaact tgtttggtgg caagtgttgg
     6961 aagtcgtagt tggtaaaggc gatcatcaag gttacgataa ctgggaagat aatcgcgaag
     7021 gtcatggcaa cataagatgg aatgatcaag aggtaaggga agccattttc ataaatccct
     7081 ttgatcatgt ctttgagtgt gcgtggaact ggaattccat tgttaatgcg ttttgcaatc
     7141 gtatgtgcat ctttgatatt tgagaaataa aagagtacat aaacgattac aaagattaga
     7201 tggaaggcac cacgaatcag cataaagagg gaattatcac gacctggttt gtcaccaaga
     7261 gtgatgaggt tgctcaattc aggggttgca agtgctagga agtaaaggac aaatacgatg
     7321 gttacaccaa ggaagataaa acctttggct ttttgtttat tgtaaatctg tcctaaccca
     7381 ggaatgatag acagcagggc tgctttacta ggttgttgct tttccatatt aactcctttc
     7441 ataggatact acttttttat aagaaagcta aattatttgt gtctctattg ataaattcaa
     7501 agggggattt gatttccacc ccccttgaac aaatttttta ttcaccaaat ttttgtttga
     7561 ttgtttcttt gatcaatgtt acagcatcgt tagcagctgt tttagcatct ttttgaccac
     7621 ttacagcatc aaagagcata tttttcgctg gatcccaaac tgcagacatt tgagagatgt
     7681 ttggcagtgg ttgagtgttc ttgaactgtt tgataacagc tgttgtcaac tcatcgtttt
     7741 taccttcagc gtatgaacga gcctcagtat tagctgggat ttcgttagtc ttatcatata
     7801 atactttttg ttgttcagtt gcaacaagga agtctacaaa tttttgagaa gcttcaaggt
     7861 tcttaacggc ttgaggaatg acccaagctt taccaccacc gaatgcagca tattcttttc
     7921 catttggaag agttgggata gttgcaactc cgtagtttac tttagcatct ttaaaggctt
     7981 gagctttcca aggtccgtcg atgatagcag ctgttttacc ttcttggaat tgagtttgga
     8041 ttaagtttcc agcaccttct gtatcttgca tacctttagg ccatttttcg taccaagatt
     8101 tagcatagtt gatacctgcg atagaaccgt cgtttgcaag accgatgtct ttagcgtctt
     8161 taccgttttg gccaaagacg taagcaccgt taccggcaag aagtccatat gtatagtaga
     8221 agtttgtcca gtcagctagg aaggcagtag ttttaccatc ttcaccagcg aatgcgtatt
     8281 tgctatcttt agcaaggttt tccaagtcag caaatgtttt tggagcatct ttcaccaagt
     8341 ctttgttgta gtacataaca agtgactcga taacggcagg agcaccgtaa actttaccat
     8401 tagcagctgt tacaagagat ttagttgtgt cgtctgtttt agcaccatcg ctcaatttca
     8461 cttctgaaag ttgtccgtca gaaccaaggc tacctacacg gtcgtatgga gccatcataa
     8521 catcagggac attaccagat tggttgtcaa gagaaagttt atcaagacct cctagagcat
     8581 caccagtttt aagagtgact tttactccag cttctttttc ataagcttta gcaacctctt
     8641 caatatagct cttatatccc tcgtctacat atacagtgag ttctttgact tcagatgaac
     8701 cagaatcagc aggcttatca gcagttttgc ttccgcaagc taccaaaagc aagctagcaa
     8761 gtgtagcagt tccaagcacc gcagtgctct tcataaattt agatgacata gtgtattcct
     8821 cccaaagaat agcaagtttt attgataagg aaacgcaaac gttttccttt atgagcttag
     8881 tatagcacaa atagaaaacg gttgcaagta ttttttgtaa aaattttaaa aaatttttaa
     8941 gcttgatttg atagcataat tttgcatttt aatagaagaa aagtgtgaaa tagataagaa
     9001 tacagtattt ttaataaaaa tatatggaat ctgtctaaag agggaaatca ggaggggcta
     9061 cctccctgtt tctaatccct atctattttc tatagacgtt tgtgctttgt tgcatataaa
     9121 ttcaagctcc ctaaggaggt tttgtacttt gtagactgtc tcgtgagagt caaaccttag
     9181 gattagatag ttttaagtta tctttgctaa aatgagttag tctccttaaa aataatcaaa
     9241 aaagtttttt aaaaacgctt gcaattatgc ttgaaaagga gtatacttat aagtaacgca
     9301 aacgtttgcg tctgcaaaat acgcaacgtt ccattatttt aacacacgag gtgctattat
     9361 gaaaaaacgt caaagtggtg tgttgatgca catctcttct cttccaggag cttacggaat
     9421 cggatcattt ggtcaaagtg cttacgactt cgttgatttc ttggtccgta caaaacaacg
     9481 ttactggcaa atccttccat taggagcaac tagttacggg gattctcctt accaatcttt
     9541 ctcagccttc gcaggaaaca ctcattttat cgatttagat atcttggtgg agcaaggttt
     9601 gttggaagca agtgaccttg aaggagttga ctttggtagc gatgcgtctg aagttgacta
     9661 tgctaaaatc tactatgcac gtcgtcctct tttagaaaaa gcggtgaaac gtttctttga
     9721 agtcggagat gttaaagatt ttgagaaatt tgctcaagac aaccaatcat ggcttgagct
     9781 ctttgctgag tatatggcta tcaaagagta ttttgacaat cttgcttgga ctgaatggcc
     9841 agatgcagat gctcgtgctc gtaaagcttc agcacttgaa agctatcgtg agcaattggc
     9901 agacaagttg gtttaccacc gtgtgactca atacttcttc ttccaacaat ggttgaaatt
     9961 gaaagcttac gctaacgaca accacatcga aatcgttggg gacatgccaa tctacgtagc
    10021 ggaagattca agtgatatgt gggcaaatcc acatctcttc aaaacagatg tcaatggtaa
    10081 ggctacttgt atcgcaggat gcccaccaga tgagttttct gtaactggtc agctttgggg
    10141 taacccaatc tatgactggg aagcaatgga caaagacggc tacaaatggt ggattgaacg
    10201 cttgcgtgaa agcttcaaaa tctacgatat cgttcgtatc gaccacttcc gtggcttcga
    10261 atcttactgg gaaatccctg ctggttccga tacagcagca cctggtgagt gggtgaaagg
    10321 tccaggctac aagctttttg cagccgttaa ggaagaactt ggtgagctaa acatcatcgc
    10381 agaagacctt ggcttcatga cagatgaagt gatcgaattg cgtgaacgta ctggcttccc
    10441 aggaatgaag attcttcaat ttgccttcaa cccagaagac gaaagcattg atagcccaca
    10501 cttggcacct gctaactcag ttatgtacac aggaacacac gataacaata cggttcttgg
    10561 ttggtaccgt aatgagattg atgatgcgac tcgtgagtac atggctcgtt acacgaaccg
    10621 taaagaatac gaaacagtgg tacacgctat gcttcgtaca gtattttcat cagttagctt
    10681 tatggcaatt gcaactatgc aagatttact agaattggat gaggcagctc gtatgaactt
    10741 cccatctacc cttggtggaa actggtcttg gcgtatgact gaagatcaat tgacaccagc
    10801 tgtcgaggaa ggtttgcttg acttgacaac aatttatcgc cgaattaatg aaaatttggt
    10861 agatttaaag aaataagaca ataatcagga gacaactaaa catgttatca ctacaagaat
    10921 ttgtacaaaa tcgttacaat aaaaccattg cagaatgtag caatgaagag ctttaccttg
    10981 ctcttcttaa ctacagcaag cttgcaagca gccaaaaacc agtcaacact ggtaagaaaa
    11041 aagtttacta catctcagct gagttcttga ttggtaaact cttgtcaaac aacttgatta
    11101 accttggtct ttacgacgat gttaaaaaag aacttgcagc tgcaggtaaa gacttgatcg
    11161 aagttgaaga agttgaattg gaaccatctc ttggtaatgg tggtttggga cgtttggctg
    11221 cctgctttat cgactcaatt gctactcttg gtttgaatgg tgacggtgtt ggtcttaact
    11281 accactttgg tcttttccaa caagttctta aaaacaacca acaagaaaca attccaaatg
    11341 catggttgac agagcaaaac tggttggttc gctcaagccg tagctaccaa gtaccatttg
    11401 cagactttac tttgacatca actctttacg atattgatgt tactggttat gaaacagcga
    11461 ctaaaaaccg cttgcgtttg tttgacttgg attcagttga ttcttctatt attaaagatg
    11521 gtatcaactt tgacaagaca gatatcgctc gcaacttaac tctcttcctt tacccagatg
    11581 atagtgaccg tcaaggtgaa ttgctccgta tcttccaaca atacttcatg gtttcaaacg
    11641 gtgcgcaatt gatcatcgac gaagcaatcg aaaaaggaag caacttgcat gaccttgctg
    11701 actacgcagt tgtccaaatc aacgatactc acccatcaat ggtgattcct gaattgattc
    11761 gtcttttgac tgcacgtggt atcgatcttg acgaagcaat ctcaattgtt cgtagcatga
    11821 ctgcctacac taaccacaca atccttgctg aagcgcttga aaaatggcct cttgaattct
    11881 tgcaagaagt ggttcctcac ttggtaccaa tcatcgaaga attggaccgt cgtgtgaagg
    11941 cagagtacaa agatccagct gttcaaatca tcgatgagag cggacgtgtt cacatggctc
    12001 acatggatat ccactacgga tacagtgtta acggggttgc agcactccat actgaaatct
    12061 tgaaaaattc tgagttgaaa gccttctacg acctttaccc agaaaagttc aacaacaaaa
    12121 caaacggtat cactttccgt cgttggctta tgcatgctaa cccaagattg tctcactact
    12181 tggatgagat tcttggagat ggttggcacc atgaagcaga tgagcttgaa aaactgttgt
    12241 cttatgaaga caaagcagct gtcaaagaaa aattggaaag catcaaggct cacaacaaac
    12301 gtaaattggc tcgtcacttg aaagaacacc aaggtgtgga aatcaatcca aattctatct
    12361 ttgatatcca aatcaaacgt cttcacgagt acaaacgcca acaaatgaac gctttgtacg
    12421 tgatccacaa ataccttgac atcaaagctg gtaacatccc tgctcgtcca atcacaatct
    12481 tctttggtgg taaagcagct ccagcctaca caatcgctca agacattatc catttaatcc
    12541 tttgcatgtc agaagttatt gctaacgatc cagcagtagc tccacacttg caagtagtta
    12601 tggttgaaaa ctacaacgtt actgcagcaa gtttccttat cccagcatgt gatatctcag
    12661 aacaaatctc acttgcttct aaagaagctt caggtactgg taacatgaaa ttcatgttga
    12721 acggagcttt gacacttggt actatggacg gtgctaacgt ggaaatcgct gagttggttg
    12781 gagaagaaaa catctacatc ttcggtgaag attcagaaac tgttatcgac ctttacgcaa
    12841 aagcagctta caaatcaagc gaattctacg ctcgtgaagc tatcaaacca ttggttgact
    12901 tcatcgttag tgatgcagtt cttgcagctg gaaacaaaga gcgcttggaa cgtctttaca
    12961 atgaattgat caacaaagac tggttcatga ctcttcttga tttggaagac tacatcaaag
    13021 tcaaagagca aatgcttgct gactacgaag accgtgacgc atggttggat aaagtcatcg
    13081 ttaacatttc taaagcagga ttcttctcat ctgaccgtac aatcgctcag tataacgaag
    13141 acatctggca cttgaactaa tactcttcga aaatctcttc aaaccacgtc agctttatct
    13201 gcaacctcaa agcggtgctt tgagcaacct gcggctagct tcctagtttg ctctttgatt
    13261 ttcattgagt ataagataca aatttatact aatacatctt gtaaaaaagc gagtttcaat
    13321 tgaaattcgc ttttttaaat gctgtggatt tgggtcaatc ttgtctaaaa atagggaaat
    13381 cctagataca gtgaaggctt taaatgctgg tttttactgt cttcagcctt atattttttc
    13441 gtagttggtt acctcacatc tattgtattc gcttacataa agtattataa tataattgta
    13501 ggaaagaagg tgtttttatg atatacacac ttaaattggt gttgtttatt atctttcttg
    13561 taataagctt gttacctgat aagatttttg gaaaaaataa aaaaatttgg aaaatagttt
    13621 ttgcaatatt gacggcagtg gcagcattgt catttatgta ctaagttatt ttaagaatgt
    13681 agggaaataa accctacatt ttttttagtt ttttctgttt tctaaattct atttatccaa
    13741 gagcttcaac atttcttgct tcttcgcttc aagttctgca cgcttttctt cgatttcggc
    13801 atgttttttc tcgagttcag aacaacttgc atcattgcta aattcttttc gccatcagga
    13861 gatagggtga gtctacatgt ctattactca cccaaagcag tcctacaaag caggaatttt
    13921 ctgttacttt tttggaaata gtaacgttta tacagctttg acacttcgta tcaaagcgcc
    13981 aaacacactc cgagggattt acagaaagca gaaaaggaat gatctggtat aagatcattc
    14041 cttttctctc tttttcttta agtaattata tacaatgtac gacgaagtcg tcattgcaat
    14101 gctgatccac cacctaaagg gaactttaaa caacattgat aagataaaga atataaacaa
    14161 cgaaaatacg ttatacccaa ttaattttat tgtatatctc atgattaaaa gttaatcctt
    14221 ccgttgttag gaatggcatc atttttatcc cataattgtg ctaaataagt ccccggtgat
    14281 aataaattca tagcggattc taaagcaaca tcatttacaa accaactacc tagatatcta
    14341 gaaattgctg aacgaatagc actttttgct gcatgttttc cttttacttt aattagattt
    14401 gcaaggcctg cagtagttcc tcctaatgct aaagctattg cagtatctaa tagagcaccc
    14461 atttgattaa ctgtaatacc ttgccaagct gctctaaatg gagagtatgt aggtgggatt
    14521 gtataatcgc cttgtaattg tcggttaatt acttctttga tccattgttg tgagacgtct
    14581 ggatgaaaag attggatttc gtttgcaagt gtattgattt gttcttctgt tagagaagtg
    14641 acaggttgaa gttccatatt tgtttcaatt tgtgatactt gttcagaagc gtatacagct
    14701 gaaacacttg gaatcgctga tacaattaac acaattgacg tcaaaaaaac cgaaataaat
    14761 ttcattaatt tgttcatgag cttttctcct ttttatttgt atctgcttac attttattat
    14821 atactgttat tatagtcaag aaaaatatgc tattatgtta aaaaaatatt tttaaaaata
    14881 taaatggacg gatttatttt ggattttatt tgttattttg acctgcctct atattggtaa
    14941 ccatgatttg tttactttca atcatcaaga attctctttt cgtggtagcg tttggggtct
    15001 ggtactggcc ttatatcact tattgttcat tgataagttt gtaatttcga atcgaaaata
    15061 aagattagaa ctatgattga ctggctactt ttaatattga ttttggagga agattttgtc
    15121 tctattattt attattttaa atttatctat tttgtacaag atttattctt tgagacggga
    15181 gaaatcgaaa tacttgattt atacggccta tatcatattt ggggtaaatg tagtatatgg
    15241 tattcaatgg ttattaagag aactgatttc aactatttcc ccttaatgtg tatggaaact
    15301 ataacaaaac gcataagatt aaggtttgtg tacctgttct tatgcgtttt tataatattg
    15361 aagattttcg gtaagtttta tacctttggg aagtctagtt tagaatgctt tcccaaccaa
    15421 aatctctgct tcgatggtaa tctctgtcag ttggctccag tcaatcttgc tctggtcaac
    15481 gtgaaagagg agaggagtca tactgagtag ggcttggagt tgttctgctg tgatagtctt
    15541 agtcagagag gcagtttgac tagataggat ggtaaagtgt tcctggaaat gctccttgat
    15601 atcttggtta gaatactcct tgtttgtcag ctggtcctgt actctttgac ggatttcttt
    15661 gaggtgattt tcagttggga taacctttat caagataccg tctttggata aaacgcgacg
    15721 aaattctcca tagttggcag gtgagaagat atcaagcaga atatccatgt tagcgtcttt
    15781 tataggaagt cgtgccaagt cgccaacaaa ccaattgact gcccagttgg gttcactttt
    15841 agcagcgatt tggactgaat ctttggagat gtcaaaggca tagaaagttt tttcagagtg
    15901 actttcttgt agtttgcgag aatagaatcc ttcaccacaa ccgatatcca aaattgttgt
    15961 ggtagttttt gagcttgcaa gcaagtcaga tacagcatct aagatagctt ggtaaaagcc
    16021 ggcttctagg atttgttgac ggttttgaaa attttccttg ttatagttag cagattgctt
    16081 gatttgaggt gctagattga catagccaaa tttcgccaag tcaaaagaat gacggttgca
    16141 gcacttgaaa ttagtctcta acagagtcag attttcttga cagataggac aggcaaaggc
    16201 agtcgcagaa gcaaaacgct gaagtttggg cttgagattt gtattcatag tttaagtgta
    16261 tcaaaagagg caaatgaaag caactttagg aataagtaga tgggagattc agtagaaata
    16321 aaactaattc tccaatttat agaatgaaat tgcttttata tctaaattgc tatataataa
    16381 tgataaggag gaaataagta tgttaaaaga agtattaacc gttgcaaaag ttgcgaaaaa
    16441 atcttcatta tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa tcttagcaag
    16501 taaggaagct aaaaaaggtt attctaaagc tttggctaag gcttacaagt tgaaagacga
    16561 gctagatgca tctgtttctg ttgtgaagca acatggagac gatgtcttgc aagatgccaa
    16621 atatttgtac gagcaagaga aaaaagaaga gcaattagat agccttatag gtgaataata
    16681 tgtcttttaa agtgctacat agaggatacc aacatatccg actatcatct tctttttcac
    16741 ttaccttgga tattcaagac tatcttcgtt ccttggcgag agatgaaaag gggattgagt
    16801 ctatccagtt ttacatggat caacagcact ttactctacg cataaaagaa ggcttttctg
    16861 tattagataa tgcagaagcc tttttaaaaa gaattgataa agggaaagtt tctgagttga
    16921 tgactcttcc cattcgtaga gaagagagtg cttattctat tgtttcaggt gcagcggtta
    16981 agcgtgtact ttttcgtagt tttgtgccgt atcctattcg ctatatatgg acttgttatc
    17041 aggctttggg ttatattaga gaagcctatc aaacactagc gcgtaaggaa ctaacgatgg
    17101 aagtcttgga ctgttcggct attttattgt ctttgtttat gaaccaatcc aagacagcta
    17161 gcaatatcat gtttatgctt gatttgggga atcatttaga tcagtggtcc ttgaaaaaaa
    17221 ctgcaacaga tttagaacag agtcttcttg caaaagagag cgatgtattc ctagtacagg
    17281 gcgatacggt tgttagtatc aagagttccg atgttcaaat aggagatgtc ttgatcttat
    17341 ctcaaggaaa tgaaattctg tttgatggac aagtagtttc aggtttaggt atggtcaacg
    17401 aaagttcctt gacaggagag agttttccag ttgaaaaaag agagtctgat ttggtttgtg
    17461 caaatacagt attagaaact ggagagttac gcattcgtgt aacagataat cagatgaaca
    17521 gccgtatttt acagctgatt gagttgatga agaaatctga agaaaacaag aaaacgaaac
    17581 aacgctattt catcaagatg gcggataaag tcgtcaaata taatttcttg ggggctgggc
    17641 tgacttacct attgacaggt tctttttcta aggctatttc tttcctattg gtcgatttct
    17701 cctgcgcttt gaaaatctct actcctgtag cttatttgac agttatcaag gaggggttga
    17761 accgtgaaat ggtgattaag gatggagatg ttctggagaa atatctggaa gttgatactt
    17821 tcttgtttga taagacagga acaatcacaa ctagttatcc tatagttgaa aaggtgttac
    17881 cttttggaga ctataataag gaagatattc tcagaatcag tgcctgtctt gaggaacaca
    17941 tttatcatcc tattgctaat gccatcgtca agcaagctga gatagaggga attgaacatg
    18001 aggaaatgca tgggaaactc caatatatcg caagcaaggg gatcaaatct catatagatg
    18061 gccaaccagt tcttattggg aattatgtct tgatgcagga tgagcagatt catatcagtt
    18121 cagaacaaaa tgctttaatt gaagagtaca agagtcacta caatctctta ttcttggctt
    18181 atcaaaatga attgattgga atgttctgca ttcatactcc tttgagaaaa gaagcaaaaa
    18241 cagccttgga taaacttaag gcacagggga aaaaattgat tctggcaaca ggagacacct
    18301 tgattaggac agaggaatta gtcaaagatt tgccctttga tcaggtctat acagacttga
    18361 aacctgatgg gaaatttgag ttagtagaga aactgcagaa agcaggtcac actatcttga
    18421 tggttggaga tggattgaat gactcagcgg ctttaactct atcagatatc ggtgtggtga
    18481 tgaatgagag tgcagatatt tctaagcaga tgagtgatat cttattgtta gataatcgtt
    18541 tggatttctt ccaagagttg gattcgttat catcatcttt gcaaacactc atcaagaaaa
    18601 atattcaaga taccgttgtc gtaaatagta gtttgattgg ctttggctta tttaattggt
    18661 tcagtccttc aaatctctct atcttacata atctaacaac cttgcgcatt gtcttgcgta
    18721 gcttgtctat taaaaataga tagatgagag ttattaacag caaaaaggag ttacctttct
    18781 cgaggttaac tcctttgttt atagataaat gttgaatagt ttagtaagtc aatacaaagt
    18841 attttttctt cccacgacgg ataacagtca gttcattttc taacttatca gcgtcactca
    18901 agacatagtc aaggtcttgg atgcggtcgc cgtttacgta gatggctccg ttttggacgt
    18961 cttcacgggc ttggcgtttt gagttaacta taccagatga gacgagcagt tccacgatat
    19021 tgttgttttc gtctgcctgt acttggtagt ttggcacacc acgaagtcct tgtttgagct
    19081 ctttgacaga aaggtttttg atgtttcctg caaagagttg ctcagtgatg ttaagtgctt
    19141 ctttgtaggc ttcttctccg tgaacaagtg taacaacttc acgagccaag actttttgag
    19201 ccaagcgttc gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc tcatcaagtg
    19261 acaagaaagt aaagattttc aagaagcgaa tagcgtcagc gtccatcacg ttcatccaga
    19321 attggtacat ttcgtatgga gaagtctttt cgggattgag ccagacggca tttccttctg
    19381 atttaccaaa ttttttacca gttgcatctg tgattagtgg aacagtgata acgtgaccag
    19441 tcttgtccgc cttacgacga agcaattcgg taccagctgt catatttccc cactggtcag
    19501 aaccaccgat ttgaagagtg acattatggt cttggttaag gacgaagaag tcgtaccctt
    19561 gcatgatttg gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt tttttaacag
    19621 attccttact catcatgtag ttgaccgtga agtattttcc aatgtcacgg aggaagtcaa
    19681 tgaagctgat gctgccaaac cagtcgtagt tgttgaccat gacagccttg ttttcgccat
    19741 tttcaaagtc aagaaaacga gaaagttgtc cttggataga cttgacccag ccatctactg
    19801 tgtcttttgt ttggagacta cgttcagcat ctttgaagga cggatctccg atgagacctg
    19861 tagcaccgcc aacgagcgca taaggtttgt gacctgctag ttgcaagcga cgacttgtca
    19921 agattgcgac aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag ccagtataat
    19981 aagaaacttg accttcttct agggctttac gcaaagcttc ttcatcagtc gtttgaaata
    20041 tcaaaccacg ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc tttttaaaat
    20101 tattgtttct acctattgta tcacaaactt gggcaatagc ctagcagaat agggaagaaa
    20161 gtggctattt tattgaaaat ttcccttttg tggtataata gataaagtga ggaaatccat
    20221 gcaaaatcaa ttaaatgaat taaaacgaaa aatgctggaa tttttccagc aaaaacaaaa
    20281 aaataaaaaa tcagctagac ctggcaagaa aggttcaagt accaaaaaat ctaaaacctt
    20341 agataagtca gccattttcc cagctatttt actgagtata aaagccttat ttaacttact
    20401 ctttgtactc ggttttctag gaggaatgtt gggagttggg attgctttgg ggtacggagt
    20461 ggccttattt gacaaggttc gggtgcctca gacagaagaa ttggtgaatc aggtcaagga
    20521 catctcttct atttcagaga ttacctattc ggacgggacg gtgattgctt ccatagagag
    20581 tgatttgttg cgcacttcta tctcatctga gcaaatttcg gaaaatctga agaaggctat
    20641 cattgcgaca gaagatgaac actttaaaga acataagggt gtagtaccca aggcggtgat
    20701 tcgtgcgacc ttggggaaat ttgtaggttt gggttcctct agtgggggtt caaccttgac
    20761 ccagcaacta attaaacagc aggtggttgg ggatgcgccg accttggctc gtaaggcggc
    20821 agagattgtg gatgctcttg ccttggaacg cgccatgaat aaagatgaga ttttaacgac
    20881 ctatctcaat gtggctccct ttggccgaaa taataaggga cagaatattg caggggctcg
    20941 gcaagcagct gagggaattt tcggtgtaga tgccagtcag ttgactgttc ctcaagcagc
    21001 atttttagca ggacttccac agagtcccat tacttactct ccttatgaaa atactgggga
    21061 gttgaagagt gatgaagacc tagaaattgg cttaagacgg gctaaggcag ttctttacag
    21121 tatgtatcgt acaggtgcat taagcaaaga cgagtattct cagtacaagg attatgacct
    21181 taaacaggac tttttaccat cgggcacggt tacaggaatt tcacgagact atttatactt
    21241 tacaactttg gcagaagctc aagaacgtat gtatgactat ctagctcaga gagacaatgt
    21301 ctccgctaag gagttgaaaa atgaggcaac tcagaagttt tatcgagatt tggcagccaa
    21361 ggaaattgaa aatggtggtt ataagattac tactaccata gatcagaaaa ttcattctgc
    21421 catgcaaagt gcggttgctg attatggcta tcttttagac gatggaacag gtcgtgtaga
    21481 agtagggaat gtcttgatgg acaaccaaac aggtgctatt ctaggctttg taggtggtcg
    21541 taattatcaa gaaaatcaaa ataatcatgc ctttgatacc aaacgttcgc cagcttctac
    21601 taccaagccc ttgctggcct acggtattgc tattgaccag ggcttgatgg gaagtgaaac
    21661 gattctatct aactatccaa caaactttgc taatggcaat ccgattatgt atgctaatag
    21721 caagggaaca ggaatgatga ccttgggaga agctctgaac tattcatgga atatccctgc
    21781 ttactggacc tatcgtatgc tccgtgaaaa gggtgttgat gtcaagggtt atatggaaaa
    21841 gatgggttac gagattcctg agtacggtat tgagagcttg ccaatgggtg gtggtattga
    21901 agtcacagtt gcccagcata ccaatggcta tcagacctta gctaataatg gagtttatca
    21961 tcagaagcat gtgatttcaa agattgaagc agcagatggt agagtggtgt atgagtatca
    22021 ggataaaccg gttcaagtct attcaaaagc tactgcgacg attatgcagg gattgctacg
    22081 agaagttcta tcctctcgtg tgacaacaac cttcaagtct aacctgactt ctttaaatcc
    22141 tactctggct aatgcagatt ggattgggaa gactggtaca accaaccaag acgaaaatat
    22201 gtggctcatg ctttcgacac ctagattaac cctaggtggc tggattgggc atgatgataa
    22261 tcattcattg tcacgtagag caggttattc taataactct aattacatgg ctcatctggt
    22321 aaatgtgatt cagcaagctt ccccaagcat ttgggggaac gagcgctttg ctttagatcc
    22381 tagtgtagtg aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga aggtttctgt
    22441 tgaagaaaaa gaagtagagg tcacaggttc gactgttacc agctattggg ctaataagtc
    22501 aggagcgcca gcgacaagtt atcgctttgc tattggcgga agtgatgcgg attatcagaa
    22561 tgcttggtct agtattgtgg ggagtctacc aactccatcc agctccagca gttcaagtag
    22621 tagttctagc gatagcagta actcaagtac tacacgacct tcttcttcaa gggcgagacg
    22681 ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcctt tcgtgttaca
    22741 ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat tcgctcctat
    22801 ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact cattatcagg
    22861 tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt gtactcattt
    22921 ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag aaaaattacc
    22981 tgcccttttt agcctatgct attttgggga ttttttcagt tcagtatttt ttctatctct
    23041 gtgtagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc cctgtcttta
    23101 tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct gttttctatg
    23161 ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagatctc tctcagttat
    23221 ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt atgtttaatg
    23281 ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt gggtggggga
    23341 tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt tcctttactc
    23401 ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg gcttttgctt
    23461 ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggtttcc gttatcagtg
    23521 ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta gtagtggatt
    23581 ggtccctcct tctagctata gtcttgatta ttttacccat gatatttttg tctatagaag
    23641 aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct atactagaat
    23701 agtcaataaa acacggggag atagttgtga agagtgtttt taggttgtat cggtaggggc
    23761 ttgcttttac cgacagcacg ttttatctca caaccctaaa aaatcatacc taaaaacaga
    23821 caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat aacgaaccag
    23881 gaggcaccta tgactgctac aaaaatgaac gctcaagaaa ttatccaatt tatcgccaat
    23941 gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac tgctgtgcct
    24001 agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt ggctccgctt
    24061 cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg taattctgca
    24121 gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc tattatccgt
    24181 gaccaagtgg aaattggtga caatgctgtt atcatgatgg gagctgttat caatatcggt
    24241 gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg ccgtgccatc
    24301 gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat tgagccagct
    24361 agtgctgaac cagtccgtgt cggagacaat gttctcatcg gggctaatgc agtggttatc
    24421 gaaggagtcc aaatcggtag tggttcagtt gtcgcagcag gagctattgt tacccaagat
    24481 gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga aattgatgcc
    24541 caaactcaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt gtaaaagtaa
    24601 aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca gatagatgtt
    24661 agatttgatt cagactagac gagatttaca ccagattcca gagattggct tggaggagtt
    24721 caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca aggattttgt
    24781 tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc agccggagcg
    24841 aaccattggt tggcggacag atattgatgg cctgccgatc gtcgaacaaa caggtctacc
    24901 ttttgcttct caacaccaag gtcgcatgca tgcctgtggc catgattttc atatgaccat
    24961 tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc tgctctttct
    25021 atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg acgatgcttt
    25081 tggagattgg ttgccagatc aattttatgg cctccatgtc cgtccagatt tgaaagtcgg
    25141 ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga agatccgttt
    25201 caaaggcaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct tggtggcggc
    25261 tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc caatcgaggg
    25321 agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca tcacagacac
    25381 agctttttta catggaacta ttcgtgcctt gacacaggac atgagtctct tggtgcaaaa
    25441 gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag tcgaagtgga
    25501 actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc gtgaactgat
    25561 ggacttcttt gacgaaaaag acggaatcga gttgattgat atcgaacctg ctatgacagg
    25621 cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc taggtatcga
    25681 tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct tagctattgg
    25741 ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg cttgtctatg
    25801 aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctatatctca agagcaaaaa
    25861 caggctatag accaagcttt aaccgagcgg cttttacacc atcccttcta ccaagaagcc
    25921 aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga actgattgag
    25981 caggcgctga aggacggcaa gaaggtttta atacccaaaa cttatcccaa ggggcgcatg
    26041 gagtttgtgg cctatgatcc gcaacagttg gtaaaaactt cctttgcctt actggagcca
    26101 cagggagatg tggaagtggt ggatgcatct cagattgatt tgattcatgt tcctggtctg
    26161 gcttttacga cgaaaggata tcggatagga tacggtggag gctattatga ccgctatctg
    26221 gaacattttt ctggtcgtac tttgagtacg atatatcctt gtcaaattca ggactttata
    26281 cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa tctttgatag
    26341 acgttaccct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt tactaatgtt
    26401 ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg gagccatgta
    26461 tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg ccatttttgt
    26521 tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc ttggaaggca
    26581 ggtagaggag attttcggtt ctaagcagtt tttctttctc tatcttttat caggaatgat
    26641 gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg cctctacctc
    26701 tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca atccttatat
    26761 ccaacagcta gggcaatcct atctgacact ttttgtggtt aacattattg gaagtgttct
    26821 gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg catttctagc
    26881 agttatcttt ccagttagag gagaaaaacg gatgtataac accagccaga gattaggagc
    26941 ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg gaatgtgacg
    27001 aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac cttatctttt
    27061 tattttattc cttctcagtc aattcttttc caagttggat gaggtaattc ttcaaatcat
    27121 ctttgacttg tggatgtttg agggcgtagt caatagatgt tttcataaag ccaaacttat
    27181 ccccaacgtc gtaacgagct cctgtaaatt cacgggcaaa aacacgctgt gttttattaa
    27241 gagtatcgat tgcatcagta agttggattt catttccagc accaggagct tggttttcga
    27301 ggatttggaa aatttcaggc gtgaggaggt agcgtccgat aatagcaagg tcgctaggag
    27361 cgtcctctgg agctggtttt tcaacaaagg tttcaacact gtaaagacca tcttttcctt
    27421 cgccttgcgg agcaataacc ccgtaagcag atacttcgtc atgagggact ggcatgacag
    27481 cgatagtaga cgcgtgggta cgcttgtagt catccatgag ttgtttggta agtggaacag
    27541 ccttttcgtc tgtgatatcc atcaagtcat caccaagcat aacgacaaaa ggttcatttc
    27601 cgacgaaagc cttggcttgc aaaacagcat ctccgagacc gcgtggatga gtttggcgga
    27661 taaaatgcag acgcatgcca gttgtttcat caactagctt caaaagatct gttttccctt
    27721 tttctttgag gttatattcc aattcgaagt ttgaatcgaa gtggtcctca atagaacgtt
    27781 ttgatttacc agtgacaact agaatatctt caatacctga tttgagagcc tcttccacga
    27841 taaactggat agttggtttg tctacgattg gcaacatttc tttggcaagg gccttggttg
    27901 ctggtaaaaa tcgagttcct agtccagcag cagggataac tgcctttcta acttttgatg
    27961 tcataaaaat cctttctata gagggttaag accactcatt ttctgcttta aattcattgt
    28021 tcatgatgtc ataaatggca tctttgatat tggttccgtg ataaataact tggtaaatag
    28081 cctgtgtaat gggcatatag actccaagtt cttgggctag ttcataggct gctcgagtcg
    28141 ttgaaattcc ttcgattacc atgcccatat tagcttctat atcagctagg gattctcctc
    28201 gtccgagagc atctccagct ctccagttac gagagtggat ggaagttccc gttacgatca
    28261 aatctcccac accagataag ccgctatagg tcaatggact ggccccgagt gctaccccta
    28321 ggcgggtgat ttctgctaaa cctcgagcga tgatggctgc cttagcatta tcaccaaatc
    28381 caagaccatg taaagctcca gcaccgacag caataatatt tttaagagca ccagcagttt
    28441 caaccccgat aacatccgta ttggtataaa gtcggaagta gtgattacta aatagctcct
    28501 gaacgtattg agctgtttgt aaatctttag aagcagcagt tattaaagtt aggtcacgca
    28561 caatggtctc ttctgcatga ctaggccctg aaacaacgac gatatcacta cggagatgtt
    28621 caggaatttc ttcttcaaga atggttgata atcgtttatg gctatcaggt tctaatccct
    28681 ttgatgcgtg catgatgata accttatggt ccaaggtttg tgcaacttgc tgggcaacaa
    28741 gtcgtgtcac ttttgttggg acaacaaaca aaatcgcatc cacatttttc aatgtttctg
    28801 ctaagtcggt gtaggcaatg atattttcgt ctagaacgac atctttaaag tagtgcttat
    28861 tagtatggtg tgtattaatt tcattgattt gctcgggaag atttccccaa atacgtacct
    28921 cgtgtccatt gtcatttaag acttgtgaaa gggcggttcc ccaagaacca ggccccaaga
    28981 cggcgacggt ttgtttttcc atgatttcct ccttgataga tttctttctc ttattctatc
    29041 atattctagg ggattttgca cttgcattac tggggtatgg tggaaaaata cacaaagacc
    29101 gagatgaagg gataaactct tagtctttag ttaaagtgag aagaaatctg atagatagtt
    29161 tgattcagta tatgcttata atagaggtaa acaactcagg aagttcttgt aggttgcgag
    29221 agtttggcga aaaaattaaa agattacgtt tggctaaaaa aatcagtcgt tcagaatttt
    29281 gtggtgatga gtctgaatta agtatccgtc aattaattag aattgaaaat ggagaatcca
    29341 gaccaacact aacaaagtta aaatatattg ctgaacgttt ggaggttgaa gattacaagt
    29401 tgatgccaag ttatatagag ttggataagg aatacctaga attgaagtat ttcttgatga
    29461 ggactcctac atacgaagat gaaactatcg cccaaaagaa agagagtgtt tttgataaga
    29521 tttttgaaga gtattatgat aggctacctg aggaagaaag atttatcatc ccaaattatt
    29581 catatctagc actagcgaac tacacagttc aaaaattacc agaaaagcta gttgaaatac
    29641 tgtccttctg gtgattttgc ttaatttctg atgttcgaga catgattgag gagatttgtc
    29701 ttgatttttt cctaaaaaag ggcagactcc tcccttggtt tgatgctcga tttttaaaat
    29761 aaggtgactt attctgttag gcagttttcc cctgccctct agctctatat ggaacaggga
    29821 gtatcagatt tatctggcag attgtatcaa ataaggcttg aaactgtttt gaataggcgt
    29881 atagacttga gaatttgaga atatgtcgtc tagcagtaga gacatatttt ccggcaatat
    29941 gaaggaagag acgtcgaaaa cgcttgatag tcaaggcctt tacttcatca ccagctagct
    30001 gctttaaaaa gaggtagaga ttataagcca gacagcccat catcatacgt acttcattct
    30061 taatcatggt cgaactatct gtcttatccc caaagaatcc tgctttcctt tctttgataa
    30121 agttttcagc attccctcgt tcacgataga attgaaacag ctcctcacta tctacactgc
    30181 ttaaattgtt caaatgaact tttttacaac cataaaaaag agcacttaat cgtactcttc
    30241 tcttttaagg attagttgaa ttaattcaaa tccaccagtt ctcctgtttc taggtagact
    30301 aggcgttcac aaatgttagc aatgtaatca gcgaagcgtt ccagatgccc tatgatataa
    30361 aggtattgag ttccattggg aattgaggtt tcttggtctt tcataagtcc aatgatttcc
    30421 tttgataagg cataataata ttggtcaatc tgttcatctt tttgagcaat actaatagct
    30481 tttgaggctt ggtgcaaagg aaaggcaacc aataaatcgg ctagcatgct gagggataat
    30541 ttacccattt ggtgtaactg ttcttcgtca ggggctagtt gattttcttt tagttgcaaa
    30601 acagctttag caatgcctgc catatggtct cccatacgtt caaggtctga acaagaagac
    30661 atgatgctaa tcacaaatcg aaggtcagac acttgtggct gctgcaaggc caacaaacgg
    30721 gcacaggtca attcgatagc gctttgacct tggttgatag catgatcctt attgataatt
    30781 agctctgcca tctccttgtc tttggaggct aaggccagta aggtttttga agctgtttca
    30841 aggacaagtt gccctagtcc taaaaaggat tgttctaatt catgcaattc taagtcaaat
    30901 tgatttctca taggtttttc cttccttatc cgaaccgtcc tgaaatatag tcttctgtgc
    30961 gctgatcttt tggattggta aacacgtcaa cggtgtctcc aaattcgcaa atttctcctg
    31021 ttaagaaaaa agcagtttta tctgaaatac gtgaagcttg ttgcatgtta tgggtaacaa
    31081 tgataatcgt ataatccttt tttagttgct gaatgaggtc ttcaattttt aaagtggaga
    31141 tagggtctaa ggctgaagtc ggctcatcca ttaacagaac atcaggttct actgctaaag
    31201 ctcgcgcaat gcaaaggcgt tgctgctgac cgcctgataa ggacatggca ctctttttaa
    31261 gattatcttt gacttcgtcc caaatggctg ccccttttaa agatttttcc actaaggcat
    31321 ctaattgttt tttgtctcga ataccatgtg tccttgggcc ataagccacg ttatcataga
    31381 tagacatggc aaagggatta ggctgttgaa aaaccatccc tacacgcttg cgtagctgat
    31441 taaggttgaa tttgctacta taaatatctt gctcatctaa gaggacttgg ccttcaatat
    31501 ggcaagaagg aaccaaatcg ttcatccgat taagggtttt tagaaaagtt gatttaccac
    31561 aaccagatgg gcctatcaag gcagtaatct gtctttctgg taattgaatc gaaatatttt
    31621 ttaaggcttg aaaatccccg taaaataaat ctaggtgtct gactgaaaat gttcccatac
    31681 taggaagctc ctttcacaag ttttcgagat aataagcttg atagagtatt tatcattaaa
    31741 acagtaataa tcaaaatcac accggtagca taggcttcat tgacatgtag cccctcactt
    31801 gacagcatat acatatgcag ggctagagaa cgacctgaag acatgagact acttggcgta
    31861 ttggtagagg tacctaatgt atacatgagg gcagctgtct caccaacgat acggccaata
    31921 gctagtatca ctccagctaa aatacctggc atggcaactg gtagaacaat tctaaaaaca
    31981 gtccgtaact tacctgcccc aagtccataa cttgcttgac gcatgctatc actaacagat
    32041 aaaagggctt cttctgttga gcgaataatg actggcaaca ccatgatgac tgaggttaag
    32101 attcctgata acagagagta ttgaaaacct aagaagacta caaagaagag catgccaaac
    32161 agaccaaaaa caatggaagg aatcccagac aaggtatctg aggccaatcg catgatttta
    32221 acacaaaggg aatctttttt tgtatattcc acaagataaa aaccagcaaa aatccctatg
    32281 ggcaaggcta aaagaagagc accaaagacc agaataacgg tggaaataat cgctggcata
    32341 agggaaatgt tctcagaagt ataagtccaa gaaaagaggg atagacttag atgaggtaaa
    32401 cctttgatga ggataaaacc aatgattaaa aagagagagc caaaggttaa agctgaaaaa
    32461 caataaacga gaagttttag caggtattta ctcataagat gattttcctt tcaagtaggc
    32521 aaagtaggca ttaatcaaga gaataaggaa aaagagaact gctgaggttg caataagggc
    32581 ttccctatgc tgacctgatg cgtaagccat ttccagaaca atattggttg ttaaggttct
    32641 ggttcctgaa aagagtccac ttggaataat cggctggttg cctgccacca aaataactgc
    32701 catggtttca cctactgcgc gaccgatgcc taaaataact gctgataaaa taccagatct
    32761 cgcagctggc aagatgacac taaaaatact ccgttcatga ctagctccta gagccaagct
    32821 accagaataa tacgttttgg gaactgttcg gatagcagat tctgacaaac tgataatggt
    32881 tggcaaaatc attattccta atagtaacga agcggttagg acactcatgc catttcctaa
    32941 aaagcttcta atccaaggca ccaataattg taggccgaaa aaaccataaa caatagatgg
    33001 aatggccgcc atcaagttga tagctgattt taagaagcca tagacgggct ttggacaata
    33061 ataaaccata aacaccgatg tcaagatgcc tgttggcacc ccaatcacaa tcgctcctaa
    33121 ggtaattaat aaggaaccaa cgatcattgg taaaatacca tagcttgccg gaatgttcgt
    33181 tggcgaccaa tcactgccta ataaaaaacg ggcaaagccg tagttagcca tgaaaggtaa
    33241 gccattacta aaaataaaga aacagattag caaaatagct acaacagcta ctgttgcact
    33301 catgaaaaaa attgccctaa aaactgcttc tttgaaggct tgttttatca catcttgtcc
    33361 tttctagtga agaaagtaag ggagatacga cacctcccta cttgccttct ttatcttatt
    33421 gtacgatgaa acgtctgcat ctctttagag atttatggag caaacatttt atttaatctt
    33481 gtcccaggtg gttaatttgc cactaaaaac gtctgcaagt tcagccatac tgacttggct
    33541 tgccttattg tcattattga ccacaacagc aataccgtct aaagcaatag catcatgggt
    33601 gagactctta ccttcttcag gagttaattc cctagaaacc ataccaatat cagcggtttt
    33661 ctccttaaca gcggtaatac ctgctgaaga cccattagag gtaatatcaa tcgtaacttc
    33721 tggattttct tttttataag cttctgctaa tttttccatt aaagaagata ctgaagtgga
    33781 acctacaaca gacaacttgc ctgataagtg ttggcttgta tattctgtgg tttcagtttt
    33841 agcttcaata aatttattat ctgtgaccac ttgttgacct tgtttggagt ggataaagct
    33901 gataaaatct tgacctagct tggaaagatt agaagaccaa acaatgttga agggacgttg
    33961 aagagggtat tcaccatcta aaactgtgtc tcgactagcc ttgacaccat caatctctaa
    34021 agccttgaca gatttcgtta aagatcccaa ggagatgtag ccgatagcat tagcattccc
    34081 ttgaactgct gagagaacac cttctgtact attttgaatc acagctgttt tggcagtgtt
    34141 gtcaattttt ttatcaccgt cttttttgag aatccctgtg atttctgtga aggcaccccg
    34201 tgttccagag ccattttctc gtgaaatcac ctcaatcgtt cctgaagctg actgtttgga
    34261 agcagctgac tgattgccac aggcaacaag cccaaatcct gataagccaa tggctgcaag
    34321 agtaagcatt tttttgaatt tcataataat cacctttatc tctatgtatt tttcttgtgt
    34381 aggcttacta catttatagt ctaacaagtc tttgtaaagg tttatccctg attcatgtaa
    34441 agattgtgta aagaatcaaa aaaagccact tttgaaaaat ggctgctcct aaaaatagct
    34501 ttaaaaatta ttagtcctgt gcgaaagatt ggttaggaag aaaaatcgtg aagcaactgc
    34561 ctctgccaag ctgactcgtc accgtgactt ggccacctaa taattgactg agttctttga
    34621 caatggcaag gccaagacca gtgccaccag tttgtctgct tcgaccttta ttaactcggt
    34681 aaaaacgttc aaaaatacga tcctgctcta attgactaat accaatccct gtatctgata
    34741 cagaaatctt aatgccttcg ttcacctttt gggtcttgac ctcaattttt cccccttgtt
    34801 cagtgtaacg gatggcattg gataaaagat tgagtaagat ttgggaaagt aattgactat
    34861 ctgatacgag ggtgacatca tctggcacct gcacctttag ctgtaaatcc ttcttcttga
    34921 gctgaggttg caagctttga gtcaaatcct gtacaaattc agccaaagaa agggtcgtcc
    34981 attgtatagg catttgttga gccttagata aggtaagaag atgctcaaca atatgctcaa
    35041 gacgcaaact ttctttgtaa ataatgtcta gaaagtcatc cttgagcgct tcttcttcag
    35101 ctgacatccc cttaatggtt tcagcaaagc ccttaattga agtaactggt gtcctcaatt
    35161 catgggaggc atttgagaca aaggctaaat ttaacttttc ataagttcta atcgttgtta
    35221 aatcatatag caagacgagc acagcttcca cagattgggt ggggctaaaa acgggaactg
    35281 ctgtcacttc taaaatcaag tcaccctcat gaaacccact tacttcttgt tttaaccttg
    35341 ttttttgatc aaaggcttgg tgaactaaat tccgaatatc catccgtttg aggtcatcaa
    35401 gtgaacttat gtcgccgtcc acatcgggaa aataatgagg cagagagcga ctggataata
    35461 acatctgacc ttgagcggaa actaaaaacg tccccatggt taggtgcgac agaagaacct
    35521 ccattgtttc ggctaggtcc ttgtattgct gatcctgttg ggagactttg gtttttaggc
    35581 cagacacata ctgagccaaa gactttaagt cttcttgccc tttttctaaa aagtattcac
    35641 tactggtcaa gagaggttgg tgcaaggtct caaaagcaac ttcccatttc caaaggcaaa
    35701 agagccagta gccaccttgt cccaaagaaa gggctagaag aaagagaccg atgcctttac
    35761 tgatccaagt taatgccatc cctgcaatca gaatgaggct aacacttaga ttgactagcc
    35821 aaaattgaag gtagcgtttc atctataact ccttgaactt ataaccataa ccccgaatgg
    35881 ttcgaataaa ttgaggggct ttaggattgt cttcaatttt ttccctcaac ttaccaatat
    35941 gaacgtccac caaacgtgtt tcctgcccaa agtcataccc ccagatacgt tccaaaagac
    36001 gctctctagt cagtgtcatg ttgggatgtt tcataagata gagcaagagt tcaaattctt
    36061 ttggggtcaa actcagtaac ttattcgcct tgtagacttc atgacgctca gggtatactt
    36121 tcaaggtccc aaatagccaa gaatcgtcag cgatattatc tgaatcatct ccttcttgtt
    36181 ctcctttagt tcgcctgagg acagccttga cacgcgccag caattctcta gggctaaaag
    36241 gcttggtcag gtagtcatca gcccctaatt ccaaggccaa aaccttatca aattcatcac
    36301 ttttcgcaga aaccatcata attggagttt tgacgccttt ggctctcagc cgcttacaaa
    36361 cttccatgcc atctaattgt ggtaacatga tatcaagcaa gataaaatca aagggttctg
    36421 tttctgccaa agctaaggcc ttccgtccat ttgttaccaa ttgagtagaa aagccttcct
    36481 tacttaaatg gtagtcaagc aatttcagaa tgtgttcttc atcatccact aataagactt
    36541 gttttgtcat ctattatctc ctattggtaa cattataaca caattatcag aaatcctaac
    36601 attgctaaat cagattaaat ttgcttatca agactagtat ctggtcaaac gctcaatcat
    36661 ctccttgtgc tctggatagg tcgccagtag atctaccctt tcaaataatt caaaatcctc
    36721 aaattcaaaa ccaggagcaa caagacaaga aaccagagca taatccttat caactgttga
    36781 tccccaaata gtgcccttag gaacacagta gtgaagttgt tgccctttgg atatgtccag
    36841 gcctaaagtg actgcttcgt agtgaccatc tgctgtaatc atgtgaacag taagtgggga
    36901 tcctgcatga aaataccaga tttcatctgc tgtcaatcgg tgaaaatgtg aaggattcgt
    36961 ttcttctaat aagaaataaa tactggtata aagcgccctt cccttaccag caaggtttat
    37021 agtgtctgaa gctttttttg tttgtctaaa atagccacct tcaacatggg gagctaactc
    37081 tagagttctt atcaagtctt ctttatccat cggggccaat gggttgaagt aactcttgtt
    37141 caaagtggtt ttacgatttc aagaactcct ctcagttctg aggacacggt aatgattgat
    37201 gcgacggaag tacaaatcaa tcgccctaaa aaaagaatta gcgaatgatt ctggtaaaaa
    37261 gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa ttgtttctat
    37321 tgccatgata tgaagttgtt caaaatgagt tgcagaaata tcggacaagc tggtaaaatc
    37381 ttggctgaca gtgattatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    37441 aaatccagca aactcaagcc gctaacagct gaagataaag cctataacca tgcgctatct
    37501 aaggaaagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatattttca
    37561 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
    37621 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt aggtgaatta
    37681 gtgaagtgtt taggcaagtg tctctggtta cgacgtcatg gcctctaaat cgattatatt
    37741 taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta gcgtctaaca
    37801 attaggaact ttagttccaa taactttaag attacgacgt tttaggacat aaatcgatca
    37861 tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat ttggcgttag
    37921 ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc tggcgcttct
    37981 actccaagca agcgaagggc ttctttgaga acgactgcgg ttgcgtagct gagggctaga
    38041 cggctgtcgc gttctgggct ttcatccaag atacgtgtat gtgcatagta tttgttaaag
    38101 gattgagcca ggctaattgc aaatttagca atgatagaag gttcaaagtt atctgccgca
    38161 cggttgataa tacgtgggaa gtcttgaatg agtttaatga tttcccagct ttcagtatca
    38221 ttcaagctat agttgccagc tgtttctggt ttgaaatcgg ctttgcgtaa gatagattgg
    38281 atacgagcgt aggcatattg aacgtaaggt ccagtttcac cctcgaagga taccatagct
    38341 tctaggtcga agtcgtatcc atttgtacgg tcagttttga ggtcatagaa tttaatggct
    38401 ccaaccccaa cagcatgtgc tacttggtct ttgttttcta gttcaggatt tttagcctcg
    38461 atttggacct tggcacggct aacagcctct gcaacagtag gctctagcaa gatgacattc
    38521 cctttacgag tagagagctt cttcccttct tttgtaacca gaccaaaagg aacgtgggta
    38581 atgtcgtcac tccaatcgta gcccatctct tgcaagacag ctttgagttg tttaaagtgg
    38641 gcagattgct cttgaccaac gacgtagata gatttagcaa attggtattc atttttacgg
    38701 tagagggctg cagccaagtc acgtgtgata tagagagttg caccatcaga cttcttgatg
    38761 agggctggat gttcaattcc atatttctca agattcacaa cttgggcacc ttctgattca
    38821 agaagtagtc ctttttcaga aagaatgtct acaactgcat ccatcttatc attgtagaag
    38881 gcttctccgt tatagctgtc aaattcaacc ttcaattcat tgtaaaggcg gttaaattcc
    38941 actaaacttt catcgcggaa ccattgccaa agagcgagag cttcctcatc tccattttca
    39001 agtttacgga accattcgcg cgcttcttca tccaagctag ggtcattttc agcttcagcg
    39061 ttgatgcgga catagagttt aaggagttca tcgattggat gagcttttac agcttcttcg
    39121 tcgccccatt ttttgtaggc aacaatcaac atcccaaatt gtttacccca gtctcccaaa
    39181 tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa gctatctccg
    39241 ataacagttg aacgcaggtg gccaatagaa aatggtttag cgatattcgg actagacatg
    39301 tcgataacaa cattttcttg tttgccaata ttttggtcag catagtgttc tttttcagtg
    39361 gtaacagctt gcaatacttg agcagaaatg gcagatttat caaggaaaaa gttaacgtaa
    39421 ggtcctgttg cgacaacttt ttcaaaggct tggctgttca ttttttcagc cagttcagcc
    39481 gcaatcattt gtggtgcttt acgttcgact ttggcaagag aaaaagcagg gaaagcaatg
    39541 tctcccattt ctgagttttt aggggtttcc agtaacttta aaatagcctc ttggtccagg
    39601 ctatcaatga tgctagataa ttcgctagca atcaattctt ttgtattcat taagagctcc
    39661 tttttggact tttctactat tttatcacaa ttttaaagaa agaagaaaaa atttttgaaa
    39721 tctcctgttt ttttggtata atatggttat aaatatagtt ataaatatgc acgcaagagg
    39781 attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact gaggagaaat
    39841 taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt gtgacgcaga
    39901 caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa aatgatatgg
    39961 tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt ttgtctcatc
    40021 atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa ttgggagaag
    40081 cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta ggaacagttg
    40141 ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa ctcatggaag
    40201 atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct caagacttat
    40261 ttttgaaaag gagagacaga aaatggcgat agaaaagcta tcacccggca tgcaacagta
    40321 tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg gtgattttta
    40381 tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt ccttaacgag
    40441 tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc attctgccca
    40501 acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag agcagatgga
    40561 agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca ttacgccagg
    40621 gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt ccatagaccg
    40681 cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact tttatgtgac
    40741 aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg ctcgagaagt
    40801 ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc agatgaatct
    40861 ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt tgcgattggc
    40921 aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga ctcagatgag
    40981 ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct tgcagatgga
    41041 ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta agaaacaagg
    41101 cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc tcttgcgttc
    41161 ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag aagtagtgca
    41221 ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca agggtgttta
    41281 tgacattgag cgcttggcta gtcgtgtttc ttttggtaaa accaatccaa aggatctctt
    41341 gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag aagggatgga
    41401 gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt tggagagttt
    41461 gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg gaattatccg
    41521 gactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag ggactagctg
    41581 gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc tcaagattga
    41641 ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactgg gaaatgtgcc
    41701 agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa ccgaagaatt
    41761 agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc tcgaatacga
    41821 aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac aagctctagc
    41881 ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg aaacccagca
    41941 tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag ggcgccatgc
    42001 tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc agatggcaga
    42061 agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta cctatatgcg
    42121 tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg ctgaaagcgc
    42181 ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg acttggtttc
    42241 gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt cgcatgcgac
    42301 caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt atgacgggat
    42361 ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta agaccctctt
    42421 tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact tggtcaatgt
    42481 ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga ttgaaccggg
    42541 accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc cagcagacct
    42601 tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag agagtcctcc
    42661 tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata gggcagaaga
    42721 gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac ctatgcaggt
    42781 tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac tagttaatct
    42841 agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat aacatcacac
    42901 aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt ttaaggagaa
    42961 agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccctggcttc gctcctgatg
    43021 acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga agtcttaggc
    43081 gccctccttg ctgggaaata tgaagctatt tatagtatcg gggcttggtt gattggtgtg
    43141 gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta tattgcccaa
    43201 ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt ttcttatgct
    43261 gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga tatcaaccag
    43321 attcagaacg ttgtcatgat gaccttccag attcttttca gacttcccct cttgttcatc
    43381 ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat tgttctcatg
    43441 gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc tcgttttgcc
    43501 aagtttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt acgtggcgtt
    43561 cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac agaggtctca
    43621 gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt ggaacccttt
    43681 atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc gggaatggtt
    43741 cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct aagccagatt
    43801 atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc catgatttcc
    43861 atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa ggatatccca
    43921 gatgaagagt tggttggaag ccttagcttt gaaaatgtga cctttaccta tccaatggac
    43981 aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat ggttggtgta
    44041 gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg tctctttgat
    44101 ccacaggaag gggctatcaa aatcggaggt aaggatattc gagaagtgag tgaaggaacc
    44161 ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg aacgattgca
    44221 gataacttga gacaggggaa gggaaatgct actctatttg aaatggagcg cgcagccaat
    44281 attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag tccagttgaa
    44341 gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc gcgtgggatt
    44401 gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga tgccaaatca
    44461 gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac cattattatt
    44521 gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa tcaaggacga
    44581 ttgattggtc aaggcacgca tgcagacttg gttgccaaca atgccgttta ccgtgaaatc
    44641 tatgaaacac agaaatgaaa gacaaactat aagaaaagtc aatagtttta tctaaactat
    44701 ttcttatttc aatttgatga tttggcgatg attttagagc acggcaaaaa gctattgaaa
    44761 aagtccattt tttcaaaggt aatcctgtgt taatttcaga aattacatca ctttttgttc
    44821 gtcaaatggc agctcttttt taggatataa aacagggttc ggataagttt ttttgcaagg
    44881 tggatgatgg ctacattgta atgttttcct tgttctaact tagtcttaag ataggcctta
    44941 aaagcaggtg aaaagcgagg gcatgctttg gcagcttgta tgagtaccta ccgcagatga
    45001 ggggaacccc gtttgaccat tcttcctgct aaatcaatct gacctgactg ataaatagaa
    45061 gaatccagtc cagcgaaagc ttgtaattga gcaggattat caaaggcatg aatatttcga
    45121 atctcggcta aaatgaccgc ccctaaacga ttctcaatcc cagtaaccgt cgtgatgacc
    45181 gagttgaact cagccatcaa gtcattgaca cattttttcg ccttgtcaat gagcctcttg
    45241 taatgtttga tgttttcatt acaagagata aaacgtttat gcgttatcaa actcattacc
    45301 aattgaaaca aaaagctgtg gttagatcct ttcggaaatt gtcaagcgat tggaggaaat
    45361 gaactaatcc acagcggctt attccaagta taccacttgg gctttggcag tagctaactg
    45421 cgctaaatat aatataagga ggagtaaaat gaagacagtt caattttttt ggcattattt
    45481 taaggtctac aagttctcat ttgtagttgt catcctgatg attgttctgg cgacttttgc
    45541 ccaagccctc tttccagtct tttctggaca agcggtgacg cagctagcca atttagttca
    45601 agcttatcaa aatggcaatc cagaacttgt atggcaaagc ctatcaggaa tcatggtcaa
    45661 tcttggcctg ctggttttgg ttctatttat ctctagtgta atatacatgt gtctcatgac
    45721 gcgcgtgatt gcagaatcga ccaacgagat gcgcaaaggc ctctttggta agcttgctca
    45781 gttgacggtt tctttctttg accgtcgaca agatggcgat atcctgtctc attttaccag
    45841 tgatttggat aatatcctcc aagcctttaa cgaaagcttg attcaggtca tgagcaatat
    45901 tgttttatac attggtctga ttcttgtcat gttttcgaga aatgtgacgc tggctctcat
    45961 caccattgcc agcaccccat tggctttcct tatgctgatt ttcatcgtga aaatggcacg
    46021 taaatacacc aacctccagc agaaagaggt agggaagctc aacgcctata tggatgagag
    46081 catctcaggc caaaaagccg tgattgtgca aggaattcaa gaggatatga tggcaggatt
    46141 tcttgaacaa aatgagcgcg tgcgcaaggc aacctttaaa ggaagaatgt tctcaggaat
    46201 tcttttccct gtcatgaatg ggatgagcct gattaataca gccatcgtca tctttgctgg
    46261 ttcggctgta cttttgaatg ataagtctat tgaaacaagt acagccctag gtttgattgt
    46321 tatgtttgca caattttcac agcagtacta ccagcctatt atccaagttg cagcgagttg
    46381 gggaagcctt cagttggcct ttactggagc tgaacgaatt caggaaatgt ttgatgcaga
    46441 ggaggaaatc cgacctgaaa aggctccaac cttcactaag ttgcaagaaa gtgttgaaat
    46501 cagtcatatc gatttttcat acttgcctga taaacctatt ttgaaagatg tcagcatttc
    46561 tgcccctaaa ggccagatga cagcagttgt tgggcctaca ggttcaggaa aaacgactat
    46621 tatgaacctc atcaatcgct tttatgatgt tgatgctggt ggtatttatt ttgatggtaa
    46681 agacattcgt ggctatgact tagatagtct tagaagcaag gtgggaattg tattgcaaga
    46741 ttcggtcttg tttagcggaa cgattcgaga caatatccga tttggtgtgc cagatgctaa
    46801 tcaggaaatg gttgaggtag cagcaaaagc aacccacatt cacgactata tcgaaagttt
    46861 gcctgataag tacgatactc ttattgatga tgaccagagc atcttttcaa cagggcagaa
    46921 gcaattgatt tcaatcgctc gaaccctgat gacagatcca gaagttctca ttctcgatga
    46981 agcaacttca aacgtagata cggtgacgga aagcaagatt cagcatgcca tggaggtggt
    47041 tgtagcaggt agaactagtt tcgtcattgc ccaccgcttg aaaaccattc tcaatgcaga
    47101 tcagattatt gtccttaaag atggagaagt cattgaacgt ggtaaccacc atgaactttt
    47161 gaagctaggt ggcttttatt cagaactcta tcacaatcaa tttgttttcg aataagaaag
    47221 aagttgtcct atgtgggcag ctttttcttg tccataaaaa atgtttatca cagccttaaa
    47281 aaaaacatat tagacgaaag tcattttgag tgatatgata ggactatcgt tagtattcga
    47341 aaggagaggc atcatggcta gaacggttgt aggagttgct gcaaatctat gtcccgtaga
    47401 cgcagaaggc aaaatcattc attcatctgt atcttgtaga ttcgcagaga tcattcgtca
    47461 agtcggtggt ctccctttag tcattcctgt tggtgatgag tcagttgtac gtgattatgt
    47521 ggaaatgatt gacaaactca ttttgacagg aggccaaaat gttcatcctc agttttatgg
    47581 agagaaaaag accgtcgaaa gcgatgatta caatctggtc cgtgacgaat ttgaattggc
    47641 actcttgaag gaagcgcttc gtcagaataa accaattatg gcaatctgtc gcggtgtcca
    47701 acttgtcaat gttgcctttg gtggaaccct caatcaagaa atcgaaggtc actggcaagg
    47761 actacctttc gggacatctc actctattga gacagtagaa ggaagcgtgg tggctaagct
    47821 atttgggaaa gaaagtcagg tcaactcagt ccatcgtcaa agcattaaag atttggcacc
    47881 taatttccgt gtaactgcta ttgattcaag agaccaaact attgaagcga ttgagtctat
    47941 cgacgagcac cgcattatcg gtttacagtg gcatccagag tttctggtta atgaagaaga
    48001 tggcaattta gaattatttg agtatttatt gaatgaactg ta
//
LOCUS       DAVKHZ010000008        43664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000008 DAVKHZ010000000
VERSION     DAVKHZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.008"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            111..3212
                     /locus_tag="VWW99_000388"
     CDS             111..3212
                     /locus_tag="VWW99_000388"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9538282.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVSYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3295..4302
                     /gene="pfkA"
                     /locus_tag="VWW99_000389"
     CDS             3295..4302
                     /gene="pfkA"
                     /locus_tag="VWW99_000389"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9538283.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4361..5866
                     /gene="pyk"
                     /locus_tag="VWW99_000390"
     CDS             4361..5866
                     /gene="pyk"
                     /locus_tag="VWW99_000390"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9538284.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6610..6835)
                     /locus_tag="VWW99_000391"
                     /pseudo
     CDS             complement(6610..6835)
                     /locus_tag="VWW99_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7683..8555
                     /locus_tag="VWW99_000392"
     CDS             7683..8555
                     /locus_tag="VWW99_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9538285.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8701..9045)
                     /locus_tag="VWW99_000393"
                     /pseudo
     CDS             complement(8701..9045)
                     /locus_tag="VWW99_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9188..9500
                     /locus_tag="VWW99_000394"
                     /pseudo
     CDS             9188..9500
                     /locus_tag="VWW99_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9504..10220
                     /locus_tag="VWW99_000395"
                     /pseudo
     CDS             9504..10220
                     /locus_tag="VWW99_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(10350..11165)
                     /locus_tag="VWW99_000396"
     CDS             complement(10350..11165)
                     /locus_tag="VWW99_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9538286.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11162..12067)
                     /locus_tag="VWW99_000397"
     CDS             complement(11162..12067)
                     /locus_tag="VWW99_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9538287.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGWAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            12479..14608
                     /locus_tag="VWW99_000398"
     CDS             12479..14608
                     /locus_tag="VWW99_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9538288.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14595..15044
                     /locus_tag="VWW99_000399"
     CDS             14595..15044
                     /locus_tag="VWW99_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9538289.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            15102..15374
                     /locus_tag="VWW99_000400"
     CDS             15102..15374
                     /locus_tag="VWW99_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9538290.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15728..15919)
                     /locus_tag="VWW99_000401"
     CDS             complement(15728..15919)
                     /locus_tag="VWW99_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538291.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16347..17105
                     /locus_tag="VWW99_000402"
     CDS             16347..17105
                     /locus_tag="VWW99_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538292.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17107..19095
                     /locus_tag="VWW99_000403"
     CDS             17107..19095
                     /locus_tag="VWW99_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538293.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTIHKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19137..19781)
                     /locus_tag="VWW99_000404"
     CDS             complement(19137..19781)
                     /locus_tag="VWW99_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9538294.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19807..20802)
                     /locus_tag="VWW99_000405"
     CDS             complement(19807..20802)
                     /locus_tag="VWW99_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9538295.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQ
                     KFSPEMMVMVKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGIKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            21509..22984
                     /locus_tag="VWW99_000406"
     CDS             21509..22984
                     /locus_tag="VWW99_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9538296.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(22950..23665)
                     /locus_tag="VWW99_000407"
                     /pseudo
     CDS             complement(22950..23665)
                     /locus_tag="VWW99_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            23752..23889
                     /locus_tag="VWW99_000408"
     CDS             23752..23889
                     /locus_tag="VWW99_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538297.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            23920..24780
                     /gene="speE"
                     /locus_tag="VWW99_000409"
     CDS             23920..24780
                     /gene="speE"
                     /locus_tag="VWW99_000409"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9538298.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            24777..26036
                     /locus_tag="VWW99_000410"
     CDS             24777..26036
                     /locus_tag="VWW99_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9538299.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            26036..27163
                     /gene="nspC"
                     /locus_tag="VWW99_000411"
     CDS             26036..27163
                     /gene="nspC"
                     /locus_tag="VWW99_000411"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9538300.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            27160..28245
                     /gene="aguA"
                     /locus_tag="VWW99_000412"
     CDS             27160..28245
                     /gene="aguA"
                     /locus_tag="VWW99_000412"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9538301.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALGKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            28255..29130
                     /gene="aguB"
                     /locus_tag="VWW99_000413"
     CDS             28255..29130
                     /gene="aguB"
                     /locus_tag="VWW99_000413"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9538302.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(29311..30120)
                     /locus_tag="VWW99_000414"
     CDS             complement(29311..30120)
                     /locus_tag="VWW99_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9538303.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            30392..30613
                     /locus_tag="VWW99_000415"
     CDS             30392..30613
                     /locus_tag="VWW99_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538304.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWQMEPVLTMP
                     VHGFCPRGTDDLGYIGGGNHLCKGSAARF"
     gene            30667..31338
                     /locus_tag="VWW99_000416"
     CDS             30667..31338
                     /locus_tag="VWW99_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9538305.1"
                     /translation="MKKRAIQILLVLSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLLLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYITSYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(31390..31587)
                     /locus_tag="VWW99_000417"
     CDS             complement(31390..31587)
                     /locus_tag="VWW99_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9538306.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFLRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            31580..32488
                     /locus_tag="VWW99_000418"
     CDS             31580..32488
                     /locus_tag="VWW99_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9538307.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            32485..32946
                     /locus_tag="VWW99_000419"
     CDS             32485..32946
                     /locus_tag="VWW99_000419"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9538308.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            32936..33823
                     /locus_tag="VWW99_000420"
     CDS             32936..33823
                     /locus_tag="VWW99_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9538309.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            33826..35709
                     /gene="cbpE"
                     /locus_tag="VWW99_000421"
                     /gene_synonym="pce"
     CDS             33826..35709
                     /gene="cbpE"
                     /locus_tag="VWW99_000421"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9538310.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNFERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIVGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            35789..36919
                     /locus_tag="VWW99_000422"
     CDS             35789..36919
                     /locus_tag="VWW99_000422"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9538311.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            36929..38191
                     /locus_tag="VWW99_000423"
     CDS             36929..38191
                     /locus_tag="VWW99_000423"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9538312.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            38195..38992
                     /gene="proC"
                     /locus_tag="VWW99_000424"
     CDS             38195..38992
                     /gene="proC"
                     /locus_tag="VWW99_000424"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9538313.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLILEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            39212..39850
                     /locus_tag="VWW99_000425"
     CDS             39212..39850
                     /locus_tag="VWW99_000425"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9538314.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            39847..40737
                     /locus_tag="VWW99_000426"
     CDS             39847..40737
                     /locus_tag="VWW99_000426"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9538315.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            40777..41094
                     /gene="yabA"
                     /locus_tag="VWW99_000427"
     CDS             40777..41094
                     /gene="yabA"
                     /locus_tag="VWW99_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9538316.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            41097..41966
                     /gene="rsmI"
                     /locus_tag="VWW99_000428"
     CDS             41097..41966
                     /gene="rsmI"
                     /locus_tag="VWW99_000428"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9538317.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            42193..42711
                     /locus_tag="VWW99_000429"
     CDS             42193..42711
                     /locus_tag="VWW99_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9538318.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            42820..43467
                     /locus_tag="VWW99_000430"
     CDS             42820..43467
                     /locus_tag="VWW99_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9538319.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKIES"
     gene            complement(43420..>43664)
                     /locus_tag="VWW99_000431"
     CDS             complement(43420..>43664)
                     /locus_tag="VWW99_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538320.1"
                     /translation="RKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKMFSTTY
                     QNHRKRFGLRMNLIAGIINHELGFYFCRKSNVILKL"
BASE COUNT        13216 a         7883 c         9626 g        12939 t
ORIGIN      
        1 tacaaatgaa tagaaaataa attagtaagc tgatgaaatt tttctcaaga gaagccattt
       61 ataggtgaaa atggtataat atagtgagaa ggatagagga gaagtgtaaa ttgatcgcac
      121 aactagatac aaaaacagtc tatagtttta tggaaagcgt catttcgatc gaaaagtatg
      181 tgagagcagc taaagaatac ggctacactc atttggctat gatggatatt gacaatcttt
      241 atggcgcttt cgactttcta gagattacaa aaaaatacgg cattcatcct ttgctagggc
      301 ttgaaatgac agtgtttgta gatgatcagg aagtgaattt gcgcttttta gctctatcta
      361 gtgtgggcta tcagcagttg atgaagcttt cgacagccaa gatgcagggg gagaaaactt
      421 ggtcagtcct gtcccagtac ctggaggata tcgcggtcat tgtgtcttat tttgatagag
      481 ttgagtcgtt agaactaggc tgtgattact atataggggt ttatccagaa acactagcaa
      541 gcgaatttca tcatcctatc ttacctcttt atcgggtcaa cgcttttgaa agcagggata
      601 gagaagttct tcaagtttta acagcgatta aagaaaatct accgctcaga gaagttccct
      661 tgcgttcgag acaagatgtc tttatatcag caagttcttt agagaaacta ttccaagagc
      721 gttttccgca agctttggac aatttagaaa agcttatttc aggcatttct tacgacttgg
      781 atactagtct gaaactgcct cgttttaatc cagctagacc agcagtagag gagttgagag
      841 agcgtgctga actggggctt gttcagaagg ggttgactag taaagaatat caagatagac
      901 tagaccaaga attgtctgtt attcatgata tgggctttga tgattatttc ttggttgttt
      961 gggatttgtt gcgttttgga cgatcgaatg gctattatat gggaatggga aggggttctg
     1021 cagtaggcag tttggtttct tatgccttag acatcacggg gattgaccca gtagagaaaa
     1081 atctgatttt tgaacgcttt cttaatcgtg aacgctatac catgcctgat attgatattg
     1141 atatcccaga tatttatcgt ccagatttta tcagatatgt tggtaataaa tatggtagta
     1201 aacatgcggc acaaatcgtt actttttcaa cctttggagc caagcaagct cttcgagatg
     1261 tcttgaaacg ctttggtgtg ccagagtatg aattatctgc aattactaag aaaatcagtt
     1321 ttcgtgacaa tcttaagtcg gcctatgagg gcaatctcca gtttcgtcag caaatcaata
     1381 gtaagttaga ataccaaaaa gcttttgaga ttgcttgcaa gatagagggc tatccaaggc
     1441 aaacctctgt ccatgcggct ggtgttgtaa ttagtgacca agatttaacc aactacattc
     1501 ctctaaagta tggtgatgaa attccactga ctcagtatga tgctcatgga gttgaggcta
     1561 gcggactttt gaagatggac tttctgggac tacgaaattt gacctttgtc cagaagatgc
     1621 aagagttgct tgctgaaaca gaaggtattc atctgaaaat tgaagaaatc gatttagaag
     1681 acaaagaaac gttagcttta tttgcctctg gtaatacaaa aggtatcttt caatttgagc
     1741 aaccaggtgc cattcgtctg cttaagcgtg tgcaaccagt ctgttttgaa gatgtcgtcg
     1801 cgactacttc tctaaatcga ccaggtgcaa gtgactacat caataatttt gtggcaagga
     1861 agcatgggca ggaagaagtg actgttctag attcagctct ggaggatatt ttggctccaa
     1921 cttatggcat tatgctctat caggagcagg ttatgcaggt tgctcagcga tttgctggat
     1981 ttagtcttgg gaaggccgat attttgcgtc gagctatggg taaaaaggat gcctctgcta
     2041 tgcatgagat gagggcttcc tttattcaag gctccataga agcaggccat actgcggaaa
     2101 aagcagagca ggtttttgat gttatggaga agtttgcagg ttatggattt aatagatctc
     2161 atgcctacgc ctactcagcc ttggccttcc agttggctta tttcaaaacg cattatccag
     2221 ccatttttta tcaggtcatg ttaaattatt ccaacagtga ttacttaata gatgcacttg
     2281 aagcaggttt tgaagtagcc tctctatcca tcaatactat tccctatcac gataaaattg
     2341 ccaacaagtc tatctatata ggtttgaaat cgattaaggg gctcagcaag gacttggcgc
     2401 tttggattat tgaacataga ccttattcta acattgaaga ttttatagct aaattacctg
     2461 agaattatct gaaacttcct ctgctagaac ctttggtaaa agttggtctt ttcgattcat
     2521 ttgaaaaaaa tcgtcaaaaa gtatttaata acttagctaa tctatttgaa tttgtgaaag
     2581 agttgggaag tttgtttgga gatgctattt atagttggca ggaatcggaa gattggacgg
     2641 aacaagaaaa attttatatg gaacaagagc ttttagggat aggtgtcagc aaacatccac
     2701 tacaagctat tgcaagtaag gctatttacc cgattacccc aatcggaaat ttgtcagaaa
     2761 atagctatgc tattattttg gttgaagttc agaaaataaa agtgattcgt accaaaaagg
     2821 gtgaaaatat ggccttctta caggcagatg atagtaagaa aaaattggat gtcactctct
     2881 tttcagactt atatcgtcag gttggacagg aaataaaaga gggagccttc tactatgtaa
     2941 aaggaaaaat acaatcacgt gatggccgtc tgcaaatgat tgcacaagaa ataagagaag
     3001 cagttgctga acgcttttgg atacaggtga aaaatcatga atcggatcaa gaaatttcac
     3061 gcattttaga acaatttaaa ggcccaatcc cagtcatcat ccggtatgaa gaggaacaga
     3121 aaaccatcgt ttctccccat cattttgtag ctaaatccaa tgaattagag gagaaattga
     3181 atgaaatcgt tatgaaaacg atttatcgct aaaaatacgg aaaatagaag aattttaaaa
     3241 tcaaatgtga tataatcaag aagaatgtta aaagaaaaag gagcataaac caatatgaaa
     3301 cgtattgctg ttttgactag tggcggagac gcacctggta tgaacgctgc catccgtgca
     3361 gttgttcgtc aagcaatttc agaaggaatg gaagtgtttg gtatctatga cggatatgct
     3421 ggtatggttg ccggtgaaat tcatccccta gatgcagctt cagtagggga catcatttct
     3481 cgtggtggta cttttcttca ctcagctcgt tatccagagt tcgctcaact tgaagggcaa
     3541 cttaaaggga ttgagcaatt gaaaaaacac ggaattgaag gtgtagttgt tatcggtggt
     3601 gacggatctt accacggcgc tatgcgtttg actgaacatg gcttcccagc tattggtctt
     3661 ccaggtacaa tcgataacga tatcgttggt actgacttta caatcggttt tgacacagcg
     3721 gttactactg ccatggacgc tatcgataag attcgtgata catcatcaag tcaccgtcgt
     3781 acttttgtaa tcgaagttat gggacgtaac gctggtgata tcgctctttg ggctggtatt
     3841 gcaactggtg ctgatgaaat catcatccct gaagcaggct tcaagatgga agatatcgta
     3901 gcaagcatca aagctggtta tgaatgtggt aaaaaacaca atatcatcgt cttagctgaa
     3961 ggtgtgatgt cagcggctga atttggtcaa aaacttaaag aagctggaga tacaagcgac
     4021 cttcgtgtaa cagaacttgg acatattcaa cgtggtggtt ctccaactgc gcgtgaccgt
     4081 gttttggcgt cacgtatggg tgcacatgct gttaaacttc ttaaagaagg tatcggtggt
     4141 gttgcggttg gtattcgtaa cgaaaaaatg gttgaaaatc caattcttgg tactgcagaa
     4201 gaaggggcat tgtttagcct tactgcagaa ggtaagattg tggttaacaa cccgcacaaa
     4261 gccgatattg agctatctag cttgaataag agcttgtcat aatttacaat ttagttaata
     4321 agaccaaaaa ggtcatatat ataaaggagt cacaaaaatc atgaacaaac gtgtaaagat
     4381 cgttgcaact ttgggtcctg cggtagaaat ccgtggtggt aaaaaattcg gtgaggacgg
     4441 atactggggt gaaaaacttg atgttgaagc ttcagctaaa aacattgcta aattgattga
     4501 agctggtgct aacacattcc gattcaactt ctcacacggc gaccaccaag aacaaggtga
     4561 gcgtatggca actgttaaac ttgcggaaaa aattgcaggt aaaaaagttg gtttccttct
     4621 tgatacaaaa ggacctgaaa tccgtacaga attgttcgaa ggtgaagcta aagaatattc
     4681 atacaaaact ggtgaaaaaa ttcgtgttgc aactaaacaa ggaatcaaat caactcgtga
     4741 agtgattgcg ttgaacgttg ctggtgctct tgatatctat gatgatgttg aagttggtcg
     4801 tcaagttttg gttgacgatg gtaaacttgg tcttcgtgtg gttgctaaag atgatgcaac
     4861 tcgtgaattt gaagttgaag ttgaaaacga tggtatcatc gctaaacaaa aaggtgtgaa
     4921 catccctaac actaaaattc ctttcccagc tcttgctgaa cgcgataacg acgatatccg
     4981 tttcggtctt gaacaaggta tcaacttcat cgcaatttca ttcgtacgta ctgcaaaaga
     5041 tgtgaacgaa gttcgtgcaa tctgtgaaga aactggaaac ggacatgttc aattgttcgc
     5101 taaaatcgaa aaccaacaag gtatcgataa cttagatgaa atcatcgaag cagctgatgg
     5161 tattatgatt gctcgtggtg atatgggtat cgaagtaccg ttcgaaatgg ttccagttta
     5221 tcaaaaaatg attatcaaga aagtcaatgc tgcaggtaaa gttgttatca ctgcaacaaa
     5281 catgcttgaa acaatgactg aaaaaccacg tgcaactcgt tcagaagtat cagacgtatt
     5341 caacgctgtt atcgacggaa ctgacgctac aatgttgtca ggcgagtctg caaacggtaa
     5401 atacccactc gagtcagtaa ctacaatggc tacaatcgac aagaacgctc aagctcttct
     5461 taatgaatac ggacgtcttg attcagattc atttgagcgt aactctaaga cagaagtaat
     5521 ggcttctgct gttaaagatg ctactagctc aatggatatc aaattggttg taactcttac
     5581 taagacaggt catactgcac gtttgatttc taaataccgt ccaaatgctg acatcttagc
     5641 attgacattt gacgaattga cagaacgtgg cttgatgttg aactggggtg ttatcccaat
     5701 gttgacagat gctccatctt caactgacga tatgttcgaa atcgctgaac gtaaagcggt
     5761 agaagcaggt ctcgttgagt caggcgatga tatcgttatc gttgctggtg tgccagtagg
     5821 agaagctgtt cgcacaaaca caatgcgtat ccgcacagta cgttaagaaa aatataaaaa
     5881 cctatcatat ccagctttct agcctgtatg atgggctctt tttttgtcca gggggcaaaa
     5941 aggggggcaa aactttcata atggattgat actcttcgaa aatcaaattc aaaccacgtc
     6001 agcgtcgcct taccgtatat atgttactga cttcgtcagt tctatctaca acctcaaagc
     6061 agtgctttga gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatg
     6121 aaataagata tgcacaaatt gattagaaag tcaaatgaat ttctacaaat gttttagcaa
     6181 tcgtaatgta cttgtctaga ttcgatctga tatattttcg atttaatgat atggtattta
     6241 aaacctccaa agtagcttgc tccattcttt tacttacgtg agtgtagatg ttatttactg
     6301 ttttagcgtt ttagcgtttt tgtgttccac tctaaccatt atagcattct tctcagctag
     6361 tgtactaagg agtgtgtgcc tgaaaatatg ggaactaagg ggctggttta tcggtttctc
     6421 tagtttagta tttgcctttt gcaaagtgat gttaaatgcc tttctctaaa tttacatatc
     6481 actattgttt aacaaaatct aatctatttt aggtcactta ttcttttttt gaaatgtaga
     6541 atgaactttt tcaaagtttt tcgaatcttt taaaatttgt ttgttttata tcgccattct
     6601 cccccttttt taattctccc tatatagcct gacaactttc ccgatggtac gaatatggtt
     6661 gctttcgtct aggtggatgt cggggtattt gggattgagt ttttttaagg tagccttggc
     6721 ggagtttctt gacatagtta gcgccgtcta cttggaagat gccgatggta ttatagtcaa
     6781 tctgtggggt attcttgata aataggtagt cgctgtttct tatctttggc tccatggact
     6841 tgctgacgac ataagcgatt gggtcgtagt cgtctgggat aatggaaact ccatatctaa
     6901 atcgttgtcc tgcatcgagc ggctacctgc agagataaac tacctaacac gagagtaagt
     6961 agtctgtctg tagtcgtcca gtctgatgat ttttacgata cttcgttttt ctgatcatac
     7021 agttgcctct cggcataggt cagaacttta ccttgtctgg gtggtgcccg ttggtcgtag
     7081 atagattgga tatcgctagg agaatccttt tgaactggag aaaagagggc atcaatcaag
     7141 ctactgaata ctttaactaa gtcaaatata gtatttttct tagtagacct aacccttttt
     7201 tcataatttc taatggtgtt tttacttata cctatcttag tacccaattc ttattgagtc
     7261 caaccattac tagtctatat tgttttatag ttgattgagt ttggaatagt acgctgtagc
     7321 tgctaaaaca tttctaggaa ttaatttgcc tttcctaata gagttgttca tatcttattt
     7381 caatctacta tatttttcgc ctctaacaat cgcaatctct tctttatcta cgaatgaaat
     7441 cgctttctat ttttgtaagt aaagcatcac acgaaatcca cgaaaatgaa aacctttgtt
     7501 gtgttttcgt aaaaaatttg ttgacagagc acgaaacgcc tactataatt aaatcaagct
     7561 taaggaaata acaaaaaaac aaatcggagg gaaagacgat gaacacatta aatgagaaag
     7621 taatcaatat ctgtaaagca gtagttaaag aaactttaat ccaagacatt taggagaaag
     7681 aaatgaagaa atcaagaaaa ttagccacat taggaatctg ttcagccttg tttttaggct
     7741 tggctgcttg tcaacaacaa catgctactt ctgaggggac gaatcaaagg caaagcagtt
     7801 cagcgaaagt tccatggaaa gcttcataca ccaacctaaa caaccaggta agtacagaag
     7861 aggtcaaatc tctcttatca gctcacttgg atccaaatag tgttgatgca ttttttaatc
     7921 tcgttaatga ctataatacc attgtcggct caactggctt atcaggagat ttcacttcct
     7981 ttactcacac cgaatacgat gttgagaaaa tcagtcatct ctggaatcaa aagaagggcg
     8041 atttcgttgg gaccaactgc cgtatcaata gttattgtct tttgaaaaat tcagtcacca
     8101 ttccaaagct tgaaaagaat gaccagttgc ttttcctaga taatgatgcg attgataaag
     8161 gaaaggtctt tgattcacaa gataaggaag agtttgatat tctattttcg agagttccaa
     8221 ctgaggcaac tacagatgtc aaggttcacg ctgaaaagat ggaaacattc ttctcacaat
     8281 ttcaattcaa tgaaaaagct cgaatgctgt ctgtagtctt gcacgacaat ctggatggcg
     8341 agtatctgtt tgtaggccac gttggggtct tagtacctgc tgatgacggt ttcttatttg
     8401 tagagaaatt gactttcgaa gagccctacc aagcgattaa atttgctagt aaggaagatt
     8461 gctacaagta tttaggcacc aagtatgcgg attatacagg cgagggactg gctaagcctt
     8521 ttatcatgga taatgataag tgggttaaac tttaatagtg ttatgatatg acagtaatct
     8581 taggattttt atttctattt ttgatgagtt ggtgggttat ttcaatcgtt aatagccaga
     8641 aaccacgcaa aaatgaaaca tttattggct atatccaaag atacgatatt gatgggaatg
     8701 gctatcaatc attaccgtgt cctcagaact gagaggagtt cttgaaatcg taaaactact
     8761 ttgaacaaga gttacttcaa cccattggct ccgacggatt aagttgcttt cgtgaacacc
     8821 aaaatcagcc gcaatttctt cataagtgcg gtattctcgc acatattgaa gagtggccat
     8881 aagaaggtct tctaggctta atttaggttt tcgtccccct tttgtgtgtt taagttgata
     8941 agcagttttt aatacagcta acatctcttc aaaagtggtg cgctgaacac caacaagaca
     9001 tttaaatcgt gcatcagtta gttgcttact tgcttcataa ttcatagaac tactatacca
     9061 tattttgttt cgcaggaagt ctaatgctaa aatcttatca gtgagaagat ttcttaaagt
     9121 ttattctttg tagtttcatc tgtattagta cataatttaa cttagagaaa ataggaggac
     9181 taatcgaatg gaattaaaag attttacaga aaaagaacag gaaatgatta agaaaaggct
     9241 tacaatgtct aatattagtg acaaagaaac tactgagaag attctggcgc tagtaccaca
     9301 agacttgatt aagcgaatcc cgttttttgt cagaaaacat gctacaacac gtacgattaa
     9361 acgcatttca attgaacacc ctgaactcta cgctgtagct caaacaagtg gtgagattcc
     9421 agaaaaaaaa acgcgaagaa ttgcgtcaga ttatcacaac tatctttgaa caaaagatga
     9481 ataagcatag tattaagtag agaatgaaaa aatattttat tggcggtttg ggaagcaatg
     9541 cctatcatag caaggatttt ctttaagaac tagattcgca ggtctatttt ctaaatccat
     9601 atgaaaagca tcttcgagat gaaacagaat tgaaatcatg gtttaaaaat gagattgtag
     9661 aggaagaatc tatctgtctg ataggtcatt ctattggagg cgatttagct cgttatttcg
     9721 catcggaatt tgaagaagta aagaaactga ttcttttgga tggtggctat ctagatttag
     9781 ataagatttt acctttggat acagagttag aggaaactaa aaattatatc aaatctcaaa
     9841 ttatttcgga cttagatgtt cttacttcta aagaaaaatc tgaagcaaag cattggtcag
     9901 aaaatatgga gaaagctgta agacagtcct atcactggaa tgttgagtat aatagatatg
     9961 agttggctat aaattatgaa aatatagaag cgatactccg cctacggaga aaaatacaag
    10021 cttttaagag agaagtggga gataccttgt ttattagtcc tcgctatcct aatgaagcta
    10081 catggagaga ggaagcccta aaagaattgc cagactattt tgatactatt tttttagaaa
    10141 actttggcca tgagctttat actcaagcac ctaaagaaat cgctagtcta atgaatgagt
    10201 ggctcgctta ttttctatga gctacttgta ttgctggaaa tggtataaga tgaaaatcta
    10261 caagtcacta attttcatat aaccaaaaaa cgaacaggct ctttttctgt tattcagaag
    10321 agaacttgtt cgtttttatc ttgattttct taaaaagctc tatatacata gggattttct
    10381 ggtttatcga ctttggtaaa gctgtcgatt tccaaggttg gaaggttttg tttgagttct
    10441 ttatggtagt gattgaccag ttttcctttg gctgaaatcc aagtgttgtt ttcatactgc
    10501 cgggtattgc ccttggtcag caatccatag acaccagaat ctgcgataca gtggataatg
    10561 ccgaatcgga acagaaattg actattggca tgactggggt cgttatagac aaagcctgta
    10621 aactggattg tcttgccctc aaactcatct ggatagtcgt agatagcctc catgacttcc
    10681 atatagtttt cattagtgat ctgaatgctg tcttgggata agtatttatc cgccgccgtt
    10741 cgcatttcct tttcataggc tgattttgaa aaataagaac tagtatctgg tttcaaatat
    10801 tggcttgtag tcccttcact tgtctgaata gccagattcg ttccttccga tagagggaaa
    10861 tgatagcctt tagctgaaac agtctgagaa tccaagctaa cagttgggaa agttaagccg
    10921 atgacaagtg gaatagccag aagagaaata ctcgtcatct tggccaatcg gctgttcaga
    10981 tgactgtggg ttttgacttg cttcatccag atatacaatt gaacgatagc caagataaaa
    11041 gaaagcacca tggagatata ggccagatag gaatagtgca tgttgatgta ctggtttagt
    11101 ttgcccgaca gatggaggta aatagtcagt tcaaaatagc cagctaaaac taaaaatcga
    11161 atcataggat gactccaatc aagagagaat agactaagac gacaagagtt acaattgtta
    11221 tgaagtgact gataaatcgt gctttcaagt aatttttcat catgagaata tttttgatat
    11281 ccagcattgg gccaatgacg agaaaggcca gaactggtgc ccaaccgaaa ctcgagagta
    11341 gagaagcacc tataaaggca tccgcctcac tacagagcga aagaagaaag gccaaaagca
    11401 tcaagagcag gatggcaaaa agaggagtcg cactgataga ggtcagaatc cgagtcggaa
    11461 cgtagacctg tattatagaa gcaaagaggc agccaaatac caaataacgc cccgtatcaa
    11521 aaaattcatc aatggcctgc acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag
    11581 aaaaatcatg ctcatgacaa gccagacgat tttctttctg aatcggttct tgccagaaaa
    11641 atcctagaaa aattcctagt attacagcca caagaatgga acccagagct cgtaataggg
    11701 cgacatggaa ggagttgcca aaggcagaat aggtcgcaaa aagaacaatg ggattgataa
    11761 caggtgctgt cacaagaaaa ggaacggccg tgtaacttgg aacctttttt tccagaaaac
    11821 gattgatgat ggggacgatt ccacattcac aagaagggaa aagtataccg acaaaggtcc
    11881 caaaaaagat tctcccccaa cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga
    11941 tataaacttc aatcagccct gagacaatgc ttcctatcag aacgaaggga agggcttcaa
    12001 tgataatgga gagaaaaatg gctccagttt gaagtacact agatgggagt tgttgaaaag
    12061 cagtcatcta gtttttcttt tctgctttga cttttttctc tttgtcatca gggaaagtaa
    12121 cttgtttcaa atcagggagt ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt
    12181 ttgtaaattt gacagccata ggtgtacctc gattctaaat tctacctcta ttatactatt
    12241 attttgcgga aaagggagtt ttttaatttg ttttcgatag ttgatacact tcgaaaatct
    12301 cttcaaacca cgtcagcttc gccttaccgt agtacggtta ctgacttcgt cagttctatc
    12361 cacaacctca aaacagtatt ttgagcagcc tacggctagc ttcctagttt gctttttgat
    12421 tttcattgag tatgaaaagg attggagaaa gccttccagc ttgtggtata atagatttat
    12481 ggataaaaaa tatgaaaaaa tctctcagga tttgggagtg acgttaaagc aaattgatac
    12541 cgttctaagt ttgacagctg aaggggcgac tattcccttt atcgcgcgtt atcgcaagga
    12601 catgactggt agtctggatg aggtggcgat taaggctatt attgatttgg ataaaagtct
    12661 gacaaatctc aatgaccgta aggaagctgt cttagctaag attcaagaac aaggtaagtt
    12721 gaccaaggaa ttggaagaag ctatcttagt tgccgaaaaa ttagcagacg ttgaagaact
    12781 ctatcttcct tataaggaaa agcgtcgtac caaggcaacc attgcccgtg aagctggact
    12841 ctttcctctt gctcgcttga ttttgcagaa tatagttgac ttagagaaag aagctgaaaa
    12901 gttcgtctgt gaaggatttg cgactggcaa ggaagccttg accggtgcag ttgatatttt
    12961 ggtcgaagcc ttatcggaag atgtgacctt gcgttctatg acttatcagg aagtgctgag
    13021 acactctaaa cttacttctc aagccaagga tgaaagtctt gatgaaaagc aggtttttca
    13081 gatttattat gatttttcag agacagttgg aactatgcaa ggctatcgta ccttggctct
    13141 caatcgtggg gagaaacttg gtgtcttgaa gatcggtttt gaacatgcga cggaccgtat
    13201 tcttgccttc tttgctactc gtttcaaggt gaaaaatgct tatattgatg aagttgttca
    13261 gcaatccgtt aagaaaaagg tcttgcctgc tatcgagcgt cgtattcgga cagaattaac
    13321 tgagaaagct gaagaaggag ctatccaact tttttctgac aatctgcgca atctcctctt
    13381 ggttgctcca ctgaaagggc gcgtggttct tggatttgac ccagcctttc gtacaggtgc
    13441 caagctagct gtcgtggatg caacaggaaa aatgctgaca actcaggtta tttatcctgt
    13501 taaaccagca tcagctcgtc aaatcgaaga agccaagaaa gatttagcag atttaattgg
    13561 tcaatacggt gtagagatta ttgccattgg aaatggaacg gccagtcgtg aaagtgaagc
    13621 ttttgtagcg gaagttctga aagatttccc tgaagtcagc tatgttatcg ttaatgaaag
    13681 tggtgcttct gtctattctg ccagcgaact tgctcgtcag gagtttccag acttgaccgt
    13741 tgaaaaacgc tctgccattt ctatcgcccg tcgtttgcaa gatcctcttg cggaattggt
    13801 caaaatcgat cctaagtcaa ttggtgtcgg tcaataccaa cacgatgtca gtcagaagaa
    13861 actatctgag agtctggact ttgttgtcga tacagtggtt aaccaagttg gtgtcaatgt
    13921 caatacagct agcccagctc ttctttcaca cgtagctgga ctcaacaaaa ctatctctga
    13981 aaatattgtc aaataccgcg aggaagaagg aaaaatcact tcacgcgccc aaatcaagaa
    14041 agttcctcgt ctgggagcca aggcctttga gcaggctgct ggtttccttc gtatccctga
    14101 aagtagcaat atccttgata atacaggagt tcacccagag aattacactg cagttaagga
    14161 actcttcaaa cgtttagata ttaaagacct aaacgaagaa gcccaaagca aactcaagtc
    14221 gctttcagtc aaggaaatgg cgcaagagtt agaccttggt ccagaaactc ttaaagatat
    14281 cattgcagat cttctcaaac caggtcgaga tttccgtgat tcctttgacg cacctgtgct
    14341 tcgccaagat gtcctagata tcaaggactt agtggttgga caaaagctag aaggagtggt
    14401 gcgtaacgtt gttgatttcg gtgcttttgt tgatatcgga attcatgagg acggtctcat
    14461 ccatatttcc catatgagtc gcaagtttat caaacaccct agtcaagtgg tggcagttgg
    14521 agatttggtg agcgtttggg ttaagaaaat tgatactgaa cgtgaaaaag ttaatctgtc
    14581 tctcctagct ccaaatgaaa ctgactgatt acgtcaagca ggtttcacta gaagacttcg
    14641 gcagaccttt tatccatcat gtccagtgga ataggcgtct acgttcgaca ggtgggcgat
    14701 ttttccccaa agatgggcat ttggatttta atcccaaggt ttatcaggaa ctggggttgg
    14761 acgtttttag gaaaattgtc cgacatgaac tctgtcatta tcacctctat tttcaaggga
    14821 aaggctatca acacaaggat cgggatttta aggaactgtt gaaagcagtg gatggattac
    14881 ggtttgtacc atccttgccc aatagtaact ccaagccaat caagctctat cgttgccaat
    14941 cctgccagca aaggtatcag cgtaagcgta ggattgatac ccaacgctat cgctgtggac
    15001 tttgtcgagg aaagttgcta ttagtaaatc agcctgagga ctgatgaaag ccgagcctat
    15061 ccgtgatata ctagtctaac ctagcagaaa gaggaaatat catgaacagt aagttttata
    15121 aaatgagacg aaatcgcatg atttcaggag ttttagcagg tctgtcagac aagtggaact
    15181 ttgatgttac ccttgtacgt tttctgtttg ccatttttac agtcgcaaac tttggacttg
    15241 gggtgattat ctatatcatc ctagcttcta tcatgccaac caaggaagaa atcgaagcag
    15301 aaatgtacgg aacaggccca cgtaaacgca aagaagctca agccattgac gataatgaag
    15361 gctggttttg gtgaggtttt acaaaagatt tggaatacgc aaaaagcttt aaatcaatag
    15421 tctaaactgc ttgatttaaa gcttttttac ttttatactc aataaaaatc aaagtgcaaa
    15481 ctaggaagcc agccgcaggc tgtaatttag tcggcaatgt gaagttgata tagtttgcac
    15541 tttgattttt gaatagtatt aataatattt cgccagcttc atccacaaac tatagtggat
    15601 tggaactaga ataggacacc tatacttcta aaacattgtt agaaatcgat ttgactgtcc
    15661 tgatcgattt gtcctgttct tatttcattt tactataaaa gcagtaattt aaacgcagtt
    15721 ttttaatcta tttttgctgt tgagacaata catttttgga aaaatggtgg atgctgataa
    15781 caactgataa ccagcaaata acataagcag tgccggtaaa ttctgtactg ataaagtgtg
    15841 tgtgttcaac taagagctga ccgatgaatg gagagagcag atagagaagt ccgatgagta
    15901 tatgttcttt gtgtttcatt aaagcgcacc ttctttcctt ataggttgaa cgacttgcaa
    15961 gtgttaaatt gaatggtatg ttatctaggt aagcttacta aaatgttagt tcccgcttcc
    16021 tccttttcaa agtaagcctt ttcacactta tattttacaa ctttaaaaat aggaaatcaa
    16081 atcattttct aagtaatttt gcttatattt cacccaatct tacaaaaatg taagattaaa
    16141 gtctcttttg taaggttagg ttatggcaag cggtcttttt ttgatgtaaa ctagactcat
    16201 aaagaacaaa ggagtaacat catgaaacaa atcttacata agaaaactag aaaaccaagt
    16261 cacaaagaaa ttgagcgcgt tcagctggga tgcactatga tgcaagctac atttcatttg
    16321 atgggatatt aagaaaggag attttcatga cacttttaga tgtaaaacac gttcaaaaaa
    16381 tttataaaac acgttttcag ggcaaccaag tagaagccct caaggatatt cactttaccg
    16441 tagaaaaggg tgactacgtt gccatcatgg gtgagtctgg ttctggtaaa tcaactcttc
    16501 tcaatattct agctatgttg gataaaccaa gtcgtggtca ggtttacttg aatggaactg
    16561 acaccgcaac tattaaaaat tcacaggctt ctagtttccg gcgtgaaaag ctaggatttg
    16621 tcttccaaga ctttaacttg ctagatactc tgtctgttaa ggacaatatc ttgcttccgc
    16681 ttgtcttgtc aagaagacct ataacggaga tgatgaagaa attggtggtg acagctgaga
    16741 atctgggtat taaccaattg caagagaagt acccttacga gatttctggt ggtcagaaac
    16801 agcgtgtagc agtagcccgc gccatcatca cagaacctga aattctcctt gcggacgagc
    16861 caacaggagc ccttgattcc aagtcatctg cagccttact tgatgtcttt aatgaaatca
    16921 atgagcgtgg gcaaaccatc ctcatggtaa cccactcaac agcagctgct agcagggcca
    16981 agcgtgttct ctttatcaaa gacggcattc tttacaacca aatctaccgt ggagagaaga
    17041 cagagcgtca gatgttccaa gaaatctctg ataccttgac tgtcatggca agcgaggtga
    17101 attagtatgt ttcgattaac caataagtta gcggtatcga acttgattaa aaaccgcaaa
    17161 ctctactatc cctttgcact ggctgttctc ttggcagtca ccatcaccta tctcttttac
    17221 tccctaacct tcaatccaaa gattgcggaa atccgtggag gaaccaccat tcaagcaaca
    17281 cttggatttg gtatgtttgt cgttaccctt gcgtcagcca ttatcgtcct ctatgccaat
    17341 agttttgtca tgaaaaaccg ttccaaggaa ctgggtatat atggcatgtt aggcttggag
    17401 aagcgccatc taatcagtat gacctttaag gagttagtgc tatttgggat tctaactgtt
    17461 ggagcaggaa tcggtattgg agccttgttt gacaagttga ttttcgcttt cctactcaaa
    17521 ctgatgaaat tgaaggtgga actggtcgct accttccaga tgaaagttgt tattacagta
    17581 cttcttgtct ttggattgat tttcctaggc ctcatgttcc tgaatgccct tcgaatcgcc
    17641 cgtatgaatg ccctccagct ctcacgtgag aaagctagcg gagagaaaaa aggtcgcttc
    17701 cttcctctcc aaacgattct tggttccata agtttaggga ttggctatta tcttgccctt
    17761 acggtaaccg atcctcttac agccctaaca accttcttcc tagctgtttt gctggttatc
    17821 tttggtactt atctattgtt taatgcaggg attacagtct tcctacaaat cttaaagaaa
    17881 aacaagaaat actattacca acctaataac ctcatatctg tttccaactt gattttccgt
    17941 atgaagaaaa atgcggttgg actagcaacc atcgctattt tgtcaacaat ggttttggta
    18001 accatgtcag cagcgacaag cattttcaat tccgcagaat cctttaaaaa agttctaaat
    18061 cctcatgatt ttggggtttc agggcaaaat gttgaaaaag aagatttgga caaactcttg
    18121 agccagtttg caagtgacaa aggttatagt gtcaaagaga aagaagtact tcgttacagt
    18181 aactttggta ttgcaaatca agaaggaacc aagttaacta tttttgaaaa aggacaaaac
    18241 cgtgtccaac caaaaacagt tttcatggta tttgaccaaa aagattatga aaatatgact
    18301 ggtcaaaaac tgtctctatc aggaaatgag gtcggtctct ttgccaaaaa tgacggactg
    18361 aaaggacaga aagctctaac tctaaatgat catcaatttt ctgtcaaaga agaatttaat
    18421 aaagatttca ttgtcaacca tgttccaaat aagtttaata tcttgactac tgattacaat
    18481 taccttgttg ttcctgattt acaagccttt ttggatcaat tcccagattc ggctatctat
    18541 aatcagtttt acggtggtat gaatgtagat gccagcgaag aagaacaact caaggtcgct
    18601 gaggagtatg aaaactacct caatcaattt aatgctcaat tagacacaga aggtagctat
    18661 gtttatggta gcaatctagc agatgctagt gctcagatga gtgccctctt tggtggtgtc
    18721 ttctttatcg gtattttcct atccattatc tttatggtcg gaactgttct ggtcatctac
    18781 tacaaacaaa tttctgaagg ctacgaagac cgtgaacgct ttattatctt gcagaaagtc
    18841 ggtttggacc aaaagcaaat caagcaaacc atccacaaac aggttttaac tgttttcttc
    18901 cttcctttgc tctttgcctt catacatctc gcctttgcct accatatgct tagcctgatt
    18961 ttaaaagtga ttggtgtact ggatacgact atgatgttga ttgtgacctt gtctatctgc
    19021 gctatcttcc tcatcgccta tgtgctgatt ttcatgatta cttcaagaag ttatcgcaag
    19081 attgtgcaaa tgtaaaaaag atacctcgac ttcaaaatcg aggtatttct tgtattttaa
    19141 atgctgaaaa gttgttcgag caggaaggta actcccatgg tcaagagacc aatagcaagg
    19201 ttccgaatca tagctgtttt ggttggggct tttccaagtc tagcacttgt gtaaccagtg
    19261 agaagaaggg ccacaccgac aataaggacg gtagcaggga tgcggtaatc acttggaaaa
    19321 atggtcactg acagcattgg aggcaaactt ctaaggaaaa aggcaacgaa gctagaaatg
    19381 gcagcgtgcc aaggattggt aaattcttca tactcaatcc catatttttc ctctaccaga
    19441 gccttgagtg gatttttaag aaagatctta ttagtcaaga gttgggcaga agttttgaat
    19501 tctccatttt ggatataaga agcatagagg gattttttgg ctagttccct atcttggtct
    19561 agcaagagtt tttctcgcga aacggcagct tcctcggtat cttttggagt tgaaacggat
    19621 acatattctc caccagccat tgaaaaggca ccagctaaga tagccgcaaa acctgataaa
    19681 aagataatcc agatattgct cgtggcactg gcaactccga taaccacacc agcaatggaa
    19741 ataattccat cgttagcatc aagaacaccc gcacgcagga tatttaaacg acctgcaaaa
    19801 tttgaatcaa tttcgtgatt tgtttctgac gctaaatttc aagttcaagt tagccatcca
    19861 gaagtcttct ctgggtgact tgtagtccaa gcatttttta ggatagttgt taatccactt
    19921 ttcgatgaat gcgacttctt tgggagtcat tttcttgatt cccttaggta accatctacg
    19981 aatgagcctg ttgtgattct cattagttcc cctttcccaa gaggcatagg ggtgcgcata
    20041 atagatgtgc tcctcagaaa atacatcaaa taagcgattg aattccgttc cattatctgc
    20101 cgtgatggaa agaatcttgt gttgttttaa gatgagtttt agagcctgat tgaccacatc
    20161 agcactttta tttggaatca atcggatgat ctgatgtcta ctctttcgat cagtcaagac
    20221 aagcaaacag tagtttttcg ctctcgtaag tagaaccgta tcaatctcat aatgcccatt
    20281 ctccaagcga agattgatag cttcagaccg ctgttcgatg gattgaccag caggtttaaa
    20341 gttggtgcta gcctgtttct taagcgcttt tccttttcta gggtaaagca gatcctgttt
    20401 gcttaacccc aattttccat gatgaatcca atagtaaatg gttgaaattc ccacgttaac
    20461 ccctttaacc atcaccatca tttcaggcga aaatttttgg ttatgatagt ggagaatctt
    20521 ttcctttagt tccttggtca cgcttgattt cctgaccgag cgcttgcgat tgttttcata
    20581 agactgttga gcatagtcgg cagaataaac ctctttgaag cgcccttttc caagacattg
    20641 tcggactgtc ccacgcttga tttcagtgtg gatagtttga ggaacttttc caagcagaga
    20701 ggcaatttct ctatttgatt tcccttcttt tttccatctt tcgattaagc gacggctatc
    20761 gattgtcaaa tgtttggctt ttgtagtata attgtcttgc atctctgtgc ctttcttgtg
    20821 tttgtggttg aacaacaagt ataacacaga ggtgttttct tatgcctaca agagctatca
    20881 gctagttgaa ccatctaatt tttaggaggg ctgggtggct aacttcatta tagaactttc
    20941 atttacgaac atatagtaaa atgaaacaag aacagaacaa atcgatcagg acagtaaaat
    21001 ctatttctaa taatgtttta gaagcagaag tgtactattc tagtttcaat ctactatatt
    21061 tttgtttttt atcaaaaaat actttacaag ttcttaaaaa catgatatag taataaagct
    21121 tagaaaatga gatgatgttt tctagcaaat ataaacccga gtaaaaaatg cctacggaca
    21181 ggcagggttg aatgccgaag cgtggttgaa aagccacatt attgataggg ttaaaagcct
    21241 acttttataa gttgatgtta ggacacttgt cctaattcat aaatttttag tgtggtgaaa
    21301 gcacacgtca tcttgtgaaa cgatcaataa agtacgtaat atttgctact agagagttag
    21361 gaaacatcgg gaacagacat actcaacaga aaccaaaata aacacgtcag aagattgcag
    21421 agcaggtgaa aacctgctct tttttcatga gtcaaccttt agttccttag ttttcataag
    21481 gtcctaaaaa tattgaaagg agtatgtttt gaaagagtta gatcaaaacc aagccccaat
    21541 ttatgaagcc ttggtgaagt tacgcaagaa aaggattgtt ccctttgatg ttccaggtca
    21601 caagcgtgga cggggaaatc cagaacttgt cgaactctta ggagaaaaat gtgtaggcat
    21661 tgatgtcaat tcgatgaaac ccttggataa tttaggccat cctatttcga ttattcgtga
    21721 tgcagaggag ctggctgcag atgcttttgg agctagccat gcctttctaa tgattggtgg
    21781 aacaacttca tcggtgcaga ctatgattct ggcaacctgc aaggcaggag ataagattat
    21841 tctgccacga aatgtccata aatctgctat caatgcgttg gttctatgtg gtgccattcc
    21901 catctatatc gagatgagtg tagatcctaa gattggtatc gctttaggtc ttgaaaatga
    21961 ccgagtagca caggccataa aggaccatcc agatgctaag gctatcctaa tcaacaatcc
    22021 tacttactac ggcatctgtt cagacctaaa ggggttgaca gaaatggctc atgaagctgg
    22081 catgatggtt ttagtagatg aagcccatgg agcgcatttg catttcactg gtaaacttcc
    22141 aatttctgct atggatgcag gagctgatat ggcagcagtt tccatgcata agtctggtgg
    22201 gagtttgaca caaagttcgc ttcttttaat cggggagcag atgaatcccg agtacgttcg
    22261 tcagattata aacctgaccc agtctacatc tgcctcttac ttattgatgg ctagtttgga
    22321 tatttcacgt cgcaacttgg cccttcgtgg taaagagtcg tttgaggaag tcattgagct
    22381 atccgagtac gctcgtcatg aaatcaatgc cattggcggt tactatgctt actcaaaaga
    22441 gttaatagat ggtgtgtcgg tctgtgattt tgatgtaacc aagttgtcag tttacactca
    22501 gggaataggc ttaacaggta tcgaggttta tgacctcttg cgagacgaat acgacattca
    22561 gatcgagttt ggtgatatcg gcaatatctt ggcctatatt tccatcggcg accgcatcca
    22621 agacatcgag cgcttggttg gtgctctggc tgatattaag agactctatt cacgagatgg
    22681 gaaggactta atagctggag aatatattca gcccgagttg gtgctgtctc cacaagaagc
    22741 cttctattca gagagaaaaa gtttgacctt agatgagtct gttggacagg tctgtggaga
    22801 atttgtcatg tgctatcctc cagggattcc tatcttggct cctggtgaac gcattacacg
    22861 agaaattgta gactatatcc aattcgccaa ggaacgtggt tgctccctcc aagggacgga
    22921 agatccagag gtcaatcata tcaacgttat taagagaaag acaaactata agaaaagtca
    22981 atagttttat ctaaactatt tcttatttca atttgatgat ttggcgatga ttttagagca
    23041 cggcaaaaag cccttgaatt agaagcggtc aatcgcttaa tttctatcag cttatcaaat
    23101 cctgcctcaa tccttttctg aggattaggg tagcgtgtca agagttggta ggtatattct
    23161 gaatgctttc caacgatttt atccaactca ggaaagatga tatcaagaca acgagtgtat
    23221 tgtactttcc aatcagactg tttttcttga gacgatgaat atgtctagcc agtattttta
    23281 gttctacttg ccgattatcg tgttgaaatt gttcacgatt ggggtcagaa agaagtttaa
    23341 gagcgatgcc atgagcgtct ttcttatccg ttttagtctt gcgaagtgat aatgatttgg
    23401 caaattcctt gatgagcaaa ggattgtagg tgtaaacttt atatccttgt tcatgcagga
    23461 agttcagtag attaaaggca taatgtccag tatcttcaag agcgatgaga cagtcttggt
    23521 tgagctgtcg aagagacaga tctaagagtt caaaaccagc tttattattt gaaaaagtga
    23581 gtggtttaag aacagttttt ccttgaacat tcaaggctgt aacatcgtgt ttatttttag
    23641 cgatatcaat gcccacataa agcatgggag tacctccaga tgtagtattt caagtctact
    23701 tggttatcca cgaacttttt gccttgttac cttagacgag ataaaacgtc tatgcgttat
    23761 caaactcatt accaattgaa acaaaagctg tggttagagc ctttcggaaa tcgtcaagcg
    23821 attggaggaa atgaactaat ccacagtggc ttattccaag tataccactt gggctttggc
    23881 agtagctaac tgcgctaaat ataatataag gagaaataga tggatttatg gttttctgaa
    23941 gttcatactc cagatgtcaa attgtctctg agaacagcca agcaacttta cgctggaaaa
    24001 agtgaatggc aggatatcga agtcttggat acgccagctt ttgggaaaat actgatttta
    24061 aatggccatg tcttgttctc agatgcggat gatttcgtct acaatgaaat gaccgttcac
    24121 gttcccatgg ctgtccaccc aaatccaaag aaagtattgg ttattggggg tggtgacggc
    24181 ggtgttgccc aagtattaac actctatcct gaactggaac aaatcgatat cgtggaaccg
    24241 gatgagatgt tggtcgaggt ctgtcgtgag tatttcccag actttgctgc agggctagat
    24301 gatcctcgtg ttaccattta ctaccaaaat gggctacgct ttttgcgaaa ctgcgaagat
    24361 gattacgata ttatcatcaa cgatgcgaca gatccatttg gccatacgga aggactcttt
    24421 accaaggaat tctacggcaa tagttatcga gctctgaagg aagacggcat catgatttac
    24481 cagcatggga gtcccttctt tgacgaggat gagtcggcct gccgaagcat gcaccgcaag
    24541 gtcaatcaat cctttccaat cagtcgggtt tatcaggccc atattccaac tagcccagct
    24601 ggctattggt tgtttggatt tgcatcgaaa aaataccacc ctgtcaaaga ttttgacaag
    24661 gaaggttgga aaaaacgcca gcttttcaca gaatactaca ctgcaaactt acacgtggga
    24721 gcttttatgt tgcccaagta tgttgaggac attttagaag aagaggaagg aaaaaaatga
    24781 gtcgtttact agttattggt tgtgggggcg ttgcccaagt tgctatttca aaaatttgtc
    24841 aagatagcga aacatttaca gagattatga ttgctagccg taccaagtca aaatgcgatg
    24901 acttgaaagc gaagctagaa ggcaaaacaa gtactaaaat tgaaactgca gcacttgatg
    24961 ctgacaaggt agaagaggtc attaccttga ttgaaagcta caaaccagaa gctgttttga
    25021 atgtagctct gccttatcaa gatttaacca ttatggatgc ttgtttggca acaggtgttc
    25081 actatatcga tacagccaac tacgaagcag aagacacaga agaccctgag tggcgtgcta
    25141 tctacgaaaa acgttgtaag gaacttggtt ttacagccta ctttgactac tcatggcagt
    25201 gggcttatca agagaaattc aaagaagcag gcttgactgc tcttcttggt tctggttttg
    25261 acccaggtgt aactagtgtc ttttcagctt atgccctcaa acactatttt gatgaaatcc
    25321 attatatcga cattttagac tgtaatggcg gtgaccacgg ttatccattt gcaaccaact
    25381 ttaatccaga aattaatctc cgtgaggttt ctgcgccagg ttcttactgg gaagatggga
    25441 aatgggtcga agtcgaagct atgtctatca agcgtgagta tgatttccct caagttggac
    25501 aaaaagatat gtatctcctt caccatgaag aaatcgaatc attggccaag aacattccag
    25561 gtgtcaaacg cattcgtttc tttatgactt ttggtcaatc ttacttgacg cacatgaaat
    25621 gtcttgaaaa tgttggactc cttcgtacgg ataccattaa ctttaacggc caagaaattg
    25681 ttccaattca atttttgaaa gccttgcttc cagatcctgc tagccttggc ccacgtacag
    25741 tcggaaaaac caatattgga tgtatcttta caggtgtcaa agatggcgtt gaaaagacta
    25801 tctacatcta caatgtttgc gaccatcaag agtgctatgc agaagtcggg tcacaagcaa
    25861 tttcatacac gacaggcgtt ccagccatga ttgggacaaa attagtcatg aacggaactt
    25921 ggaaacaagc tggagtgtat aaccttgagg agttagatcc agatccattc atggaagctt
    25981 tgaatgagta tggtttgcca tgggttgtgg ttgaaaatcc acaaatggtg gactaatgaa
    26041 gttagaacaa gtaccaacac cagcctatgt tattgacttg gccaagttag aagctaattg
    26101 ccgcattcta caatatgtac aagaagaggc cggttgcaag gtcttgcttg cccagaaggc
    26161 atattccctc tacaaaactt atcccttgat tagccagtat ctatcaggta cgacagctag
    26221 tggactctat gaggccaaat tggcaaggga agaatttcct ggtgaagtcc atgtatttgc
    26281 gcctgctttc aaggatgcag acttggagga attgcttggg ataacagacc atatcgtctt
    26341 taactcagag agacagttgc gtaagcatgg ggctcgttgt cgagaggctg gtgtcagtgt
    26401 tggtttgcgc ctcaaccctc agtgttcaac tcaaggagat cacgcgctct atgacccttg
    26461 tgcaccaggt tctcgctttg gagttactat agacaagatt ccgagtgatt tgctagattt
    26521 ggttgacgga cttcattttc ataccctttg cgagcaggga gcagatgatt tacaaacaac
    26581 tttgaaagca gtagaagaac aatttggtcc ctacttacat gaggtaaaat ggctcaatat
    26641 gggtggtggt catcatatta caagagaagg ttacgatgtg gatttgctga tttcagaaat
    26701 caagcgtatc cgaaaaactt acaatcttga aatctatatc gagcctggtg aagccattgc
    26761 gcttaatgcg ggttatttag caactgaggt attagatatt gtagaaaacg gtatggaaat
    26821 cttggtttta gacgcctctg cgacctgcca tatgcctgat gtacttgaga tgccctatcg
    26881 tccacctttg agaaatggct ttgaggcaca ggaaaaagcc catacctaca gactttcttc
    26941 taatacctgt ctgacgggcg atgtgattgg tgattatagt tttgaaaatc cagtccaaat
    27001 cggagacaga ctttattttc aagacatggc catttattct tttgtcaaaa ataatacctt
    27061 taatggtatt ggattgccaa gtctctatct catggacgaa cagggagact gtagcttact
    27121 caaagctttt ggctatcaag actttaaagg gagattatca tgatggacag tccaaaaaaa
    27181 ttaggctatc acatgccagc agagtacgaa ccccatcatg gtaccctcat gatatggccg
    27241 actcgaccag gatcatggcc ttttcaagga aaggctgcta aaagatcatt tactcagatc
    27301 atcgagacca tagcagaagg ggaaagagtc tatcttttgg tggagcaggc ctatctatct
    27361 gaagcccaat cctatcttgg agacaaggtt gtttatttag acattcccac caatgatgcc
    27421 tgggcgcgtg atactggccc aaccattctc gtcaatgata aaggtaagaa attagccgtg
    27481 gattgggcct tcaatgcttg gggaggcacc tatgatggtc tttatcaaga ttatgaagag
    27541 gatgaccaag tagccagtcg ttttgctgag gccttgggaa agcctgtcta tgatgctaaa
    27601 ccttttgtac tggaaggagg cgcaatccat agcgatggtc aaggaactat tctcgtaact
    27661 gaaagttgct tgcttagtcc tggtcgcaat cctaacctga ctaaagagga gattgaaaac
    27721 acattattag aaagtcttgg tgctgaaaaa gttatttggc ttccttatgg tatttatcag
    27781 gatgaaacca atgaacacgt cgataatgtt gctgcctttg ttggtcctgc tgaggttgtt
    27841 ttagcttgga cagatgacga aaatgatccc cagtatgcca tgtcaaaagc agatctcgaa
    27901 ctcttagaac aggaaacaga tgcaaaaggt tgtcacttca ccattcataa attgcctatc
    27961 cctgcagttc gacaagttgt gacagaagaa gatttgccag gctacatcta tgaagaagga
    28021 gaagaaaagc gatacgcagg tgaacgacta gcagcttcct acgtaaactt ttatatcgcc
    28081 aacaaggctg tcttggttcc acagtttgag gatgtaaacg accaagtggc cttagatatc
    28141 ctcagcaagt gtttcccaga ccgtaaagtt gtcggaatac cagccagaga tattctctta
    28201 ggtggtggca atatccactg tatcacccaa caaattccag aataggagaa aaagatgaga
    28261 aatgtaagag ttgcaaccat tcagatgcaa tgcgctaagg atgtggcaac aaatatccaa
    28321 accgcagagc gtttagtacg tcaggctgct gagcaaggag cccaaattat tctcttgccc
    28381 gagttgtttg aacatcccta tttctgtcag gaacgtcagt atgactacta ccagtatgcc
    28441 caatctgtag cggaaaatac tgccattcag cattttaagg tgattgctaa ggaactacaa
    28501 gttgttttac caatcagttt ctatgaaaaa gatggtaatg tcttgtataa ctctattgcc
    28561 gtcattgatg cagatgggga agtgctgggc gtttatcgaa agacccacat accagatgac
    28621 cattattatc aagaaaaatt ctatttcacg cctggtaaca ctggtttcaa ggtctggaat
    28681 actcgctatg ctaagattgg tatcggtatc tgttgggatc aatggttccc tgaaacagcg
    28741 cgctgtcttg cattgaatgg tgctgaattg ctcttttatc ctacagctat cggttcagag
    28801 ccaattttgg atacagatag ttgtggtcac tggcaacgta ctatgcaagg gcacgcagca
    28861 gcgaatattg ttccagtcat cgcagccaat cgttatggtt tagaggaggt tactcctagt
    28921 gaggaaaatg gcggacagag ttccagtctt gacttctacg gttcctcctt tatgacggat
    28981 gaaacaggag ctattctaga acgagctgaa agacaagaag aagctgttct gttagctact
    29041 tatgacctag acaagggagc aagtgaacgc ctaaactggg gcttgtttcg agatagaaga
    29101 ccagaaatgt atagacaaat tacagattag tgtgggagaa atgagagatt cattctgcta
    29161 gactaacttc ttattagcaa ctataagata ctatggcatc tagtaaatcg atttttatga
    29221 ttcgctattc ttgtctattg attagtccgt attttaaaat attagcaaaa aagcaaatag
    29281 cagtaacttc tgtctatttg cttttctttt ttatagaata tatttctcaa tagcacgcgc
    29341 aacgccgtct tcttcgttgc ttgaggtaac ggcatccgca agagatttga cataatcgct
    29401 ggcatttccc attgcaatcc caagccctgc aaactggagc atttcgatat cgttattagc
    29461 atcgcccatg gccataatct ctgaggaatc aatcttcaaa atctcagcta gtcgtgaaag
    29521 agcagtagcc tttgtcgttc caagcggcat tgcttcataa atgacaggct gcgaacgaac
    29581 tccactgaat cgttggcaaa gctcttcagc aaaacgctgc tcaaaatcgt ctgtttgttc
    29641 ttttgttcct aaaaacatac cttggaacat ccggaacttt ccactagtcg cttcttcaag
    29701 agaaatttca gtcaggtctg aaaatactag tttagcatca ttttcaataa cttgattggg
    29761 tttgccaccg agaacaaaat aatgtgactc gtcaaaaagt gtcaactgaa catcactctt
    29821 ttcagcaagg tcatagaggt attcgatgtc agctggactc agttctttcc agtcaactag
    29881 actccaatca ctggtctggt gagttgaaca accgttgtta acaataatat attcgttctg
    29941 gaggtcgagt cccagttttt tgtagtaggg gaggacaccg aaaagggggc gacccgtaca
    30001 gagaaccagt ttgacacctt tttcaatggc tttgtgaata gcagtaatgt gagcttgtgg
    30061 gatttccttg gcttcattga ggagggtgcc gtccatatcc aaggctagta gtttaatcat
    30121 aggtcttcct tctttatctt ttgctattat tatagcatat tttggagaag aaattgatag
    30181 aaagcttgag actaattgat tttatagttt aagatgtttt gatgacaatt catgatttga
    30241 agaggatatt tggcaaagat atgctatact atgtttgtca atgttgcaac tagacaaatt
    30301 aaaaaaccaa cttaatataa tagttttttt gtaagtaggt atgagtatca gattactcaa
    30361 ctaatctgaa gaataatgga ggaaatatat catgatttta atgacaaaaa atataaatct
    30421 aacaaatgaa gaattagagc tgatacaagg tggagcagat ccctatggta aagaacctaa
    30481 tggttattat ccttggcaaa tggaaccagt attaactatg ccggttcatg gattttgtcc
    30541 cagaggaacc gatgatttag gatatattgg aggaggtaat catctttgca aaggaagtgc
    30601 tgcgagattt taagtaaaat ttattaggaa tatgaagaaa caaggggaga aaacagagga
    30661 tttaatatga aaaaacgagc tattcaaatt ttactagtat tgtccttaat tttttacaaa
    30721 tcaacttggt tttggaggct tttcaattat ctcgcaaagc cctatctacc agcaagtcgt
    30781 gaattttttc agattctgct tttgatggag agtggagttc ttttattagc ggtcatctat
    30841 ctactggttt ttgcaggaaa gaaaattttt catttcaagt ggcagctgag gtattttatc
    30901 taccttttac tgggttacat cacttcgtat atgtctgact ttctcttttc gtatttcata
    30961 tctctgtctt caaatcagat ttctttgaat gaaacggtag aaatgatggg gagacaggag
    31021 ttcccttatt tcttgctcat cgtttgcttc atcgccccta ttgctgagga attgatttat
    31081 cgaggtgtgc ttatgacaac ctttttcaaa aactcacctt ggtacggaga tgttttgctt
    31141 tctgctatta ttttcggtta tattcatatc aattttgctt taactcctct tgcttttttc
    31201 atttatgcta gtggaggtct tattttagct ctattgtatc gcatgactaa aaatctctac
    31261 tatccaatac tagttcatat tctcatcaat atcactgcct tctgggatgt ttggttactc
    31321 ctattttcag gaagttagct tactaaaaaa atgtcggaat tttccggcat tttctttttt
    31381 cacaaatagt caacgttttt ctttccgata ctgaagtggt gtgtagccac ttattttttt
    31441 gaattgattt tgaaaataag attggcgtaa gaaaggcaga tagtgaagat agttaagaag
    31501 aataggatgt tcttttttcc tttttggaaa acttctaaaa tatggtataa tgaaaagata
    31561 aagaagttgg gggtagaaga tgaacattca acaattacgc tatgttgtgg ctattgccaa
    31621 tagtggtact tttcgtgaag ctgctgaaaa gatgtatgtt agtcagccga gtctgtctat
    31681 ttctgttcgt gatttggaaa aagagttggg ctttaagatt ttccgtcgga ccagctcagg
    31741 gactttcttg acccgtcgtg ggatggaatt ttatgaaaaa gcgcaagaat tggttaaagg
    31801 atttgatatt tttcaaaatc agtatgccaa tcctgaagaa gaaaaagatg aattttccgt
    31861 tgctagccag cactatgact tcttgccacc aactattacg gccttttcag agcgctatcc
    31921 tgactataag aacttccgta tttttgaatc aactactgtt caaatattag atgaagtggc
    31981 gcaagggcat agtgagattg ggattatcta cctcaacaat caaaataaaa aggggattat
    32041 gcaacgggtt gaaaagttag gtctggaggt catcgaattg attcctttcc atacccatat
    32101 ttatctctgt gagggtcatc ctttagccca gaaagaggaa ttagtcatgg aggatttagc
    32161 ggatttacca acggttcgtt tcactcaaga gaaagacgag tacctttatt attcagagaa
    32221 ctttgttgat accagcgcta gctcacagat gtttaatgtg acagaccgtg ccaccttgaa
    32281 tggtattttg gagcggacgg acgcctatgc gacaggttct ggatttttag atagtgacag
    32341 tgttaatggc attacagtta ttcgtctcaa ggataaccta gataaccgca tggtctatgt
    32401 taaacgtgaa gaagtggagc ttagtcaagc tgggactctc ttcgtagaag tcatgcaaga
    32461 atattttgat caaaagagga aatcatgaaa aaaagagcaa tagtggcagt cattgtactg
    32521 cttttaattg ggctggatca gttggtcaaa tcctatatcg tccagcagat tccactgggt
    32581 gaagtgcgct cctggattcc caatttcgtt agcttgacct acctgcaaaa tcgaggtgca
    32641 gccttttcta tcttacaaga tcagcagctg ttattcgctg tcattactct ggttgtcgtg
    32701 ataggtgcca tttggtattt acataaacac atggaggact cattctggat ggtcttgggt
    32761 ttgactctaa taatcgcggg tggtcttgga aactttattg acagggtcag tcagggcttt
    32821 gttgtggata tgttccacct tgactttatc aactttgcaa ttttcaatgt ggcagatagc
    32881 tatctgacgg ttggagtgat tattttattg attgcaatgc taaaagagga aataaatgga
    32941 aattaaaatt gaaactggtg gtctgcgttt ggataaggct ttgtcagatt tgtcagaatt
    33001 atcacgtagt ctcgcgaatg aacaaattaa atcaggccag gtcttggtca atggtcaagt
    33061 caagaaagct aaatacacag tccaagaggg tgatgtcgtc acttaccatg tgccagaacc
    33121 agaggtatta gagtatgtgg ctgaggatct tccgctagaa atagtctacc aagatgagga
    33181 tgtggctgtc gttaacaaac ctcagggaat ggttgtgcac ccgagtgctg gtcataccag
    33241 tggaacccta gtcaatgccc tcatgtatca tattaaggac ttgtcgggta tcaatggggt
    33301 tctgcgtcca gggattgttc accgtattga taaggatacg tcaggtcttc tcatgattgc
    33361 taaaaacgat gatgcgcatc tagcacttgc ccaagaactc aaggataaaa agtctctccg
    33421 caaatattgg gcgattgttc atggaaatct gcctaatgat cgtggtgtaa ttgaagcgcc
    33481 gattggccgg agtgaaaaag accgtaagaa acaggctgta actgctaaag ggaagcctgc
    33541 agtgacgcgt tttcacgtct tggaacgctt tggcgattat agcttagtag agttgcaact
    33601 ggagacaggg cgcactcatc aaatccgtgt ccacatggct tatatcggcc attcagtcgc
    33661 tggtgatgag gtctatggtc ctcgcaagac tttgaaagga catggacaat ttcttcatgc
    33721 caagacttta ggttttactc atccgagaac aggtaagacc ttggaattta aagcagatat
    33781 cccagagatt tttaaggaaa ccttggagag attgagaaag taagaatgaa aaagaaatta
    33841 actagtttag cacttgtagg cgctttttta ggtttgtcat ggtatgggaa tgttcaggct
    33901 caagaaagtt caggaaataa aatccacttt atcaatgttc aagaaggtgg cagtgatgcg
    33961 attattcttg aaagcaatgg acattttgcc atggtggata caggagaaga ttatgatttc
    34021 ccagatggaa gtgattctcg ctatccatgg agagaaggaa ttgaaacgtc ttataagcat
    34081 gttctaacag accgtgtctt tcgtcgtttg aaggaattgg gtgtccaaaa acttgatttt
    34141 attttggtga cccataccca cagtgatcat attggaaatg ttgatgaatt actgtctacc
    34201 tatccagttg accgagtcta tcttaaaaaa tatagtgata atcgtattac taattttgaa
    34261 cgtctatggg ataatctgta cggttatgat aaggttttac agactgcttc agaaaaaggt
    34321 gtttcagtta ttcaaaacat cacacaggga gatgctcatt ttcagtttgg ggacatggat
    34381 attcaactct ataactatga aaatgaaacc gattcatcag gtgaattaaa gaaaatttgg
    34441 gatgataatt ccaattcctt gattagtgtg gtaaaagtca atggcaagaa aatttacctt
    34501 gggggcgact tagataatgt tcatggagca gaagacaagt atggtcctct cattggaaaa
    34561 gttgatttga tgaagtttaa tcatcaccat gataccaaca aatcaaatac caaggatttc
    34621 attaaaaatt tgagtccgag tttgattgtt caaacttcgg atagtctacc ttggaaaaat
    34681 ggtgttgata gtgagtatgt taattggctc aaagaacgag gaattgagag aatcaacgca
    34741 gccagcaaag actatgatgc gacagttttt gatattcgaa aagacggttt tgtcaatatt
    34801 tcaacatcct acaagcctat tccaagtttt caagctggtt ggcataagag ttcatatgga
    34861 aactggtggt atcaagcgcc tgattctaca ggagaatatg ctgtcggttg gaatgaaatt
    34921 aagggtgaat ggtattactt taaccaaacg ggtatcttgt tacagaatca atggaaaaaa
    34981 tggaacaatc gttggttcta tttgacagac tctggtgttg ctgctaaaaa ttggaagaaa
    35041 attgttggaa tctggtatta ttttaacaaa gaaaaccaga tggaaattgg ttggattcaa
    35101 gataaagagc agtggtatta tttggatgtt gatggttcta tgaagacagg atggcttcaa
    35161 tatatggggc aatggtatta ctttgctcca tcaggggaaa tgaaaatggg ctgggtaaaa
    35221 gataaagaaa cctggtacta tatggattct actggtgtca tgaagacagg tgagatagaa
    35281 gttgctggac atcattacta tctagaagaa tcaggagcta tgaagcaagg ctggcttaaa
    35341 aaggcaaatg attggtattt ctacaagaca gatggttcac gagctatagg ttggattaaa
    35401 gacaaggata aatggtactt cttgaaagaa aatggtcaat tacttgtgaa tggtaagaca
    35461 ccagaaggct atactgtgga ttcaagtggt gcctggttag tggatgttcc gatcgagaaa
    35521 tctgctacaa ttaaaactac aagtcattca gaaataaaag aatccaaaga agtagtgaaa
    35581 aaggatcttg aaaataaaga aacgagtcaa catgaaagtg ttacaaattc ttcaactagt
    35641 caagatttga catcctcaac ttcacaaagc tctgaaacga gtgtaaacaa atcggaatca
    35701 gaacagtagt agaaaagaag gttttagggc cttctttttc ctatcaactc ttttctattt
    35761 cctgttattc atgttataat ggataaatat gaataatcgg agtgagacta tgaaatacaa
    35821 acggattgtc tttaaggtgg gtacttcttc tctgacaaat gaggatggaa gtttatcacg
    35881 tagtaaggta aaggatatta cccagcagtt ggctatgctg cacgaggctg gtcatgagtt
    35941 gattttggtg tcttcaggtg ccattgcggc tggttttgga gccttaggat ttaaaaagcg
    36001 tccgactaaa attgctgata aacaggcttc agcagcggta gggcagggac ttttgttgga
    36061 agaatatacg accaatcttc tcttgcgtca aatcgtttct gcacaaatct tgctgaccca
    36121 agatgacttt gtggataagc gtcgttataa aaatgcccat caggctttgt cggttttgct
    36181 caaccgtggg gcaattccta tcataaatga gaatgatagt gtcgttattg atgagctcaa
    36241 ggttggggac aatgacactc taagtgctca agtagcggcg atggtccaag cagacctttt
    36301 agttttcttg acagatgtgg acggtctcta tactggaaat cctaattcag atccaagagc
    36361 caaacgcttg gagagaatcg agaccatcaa tcgtgagatt attgatatgg ctggtggagc
    36421 aggttcgtca aacggaactg ggggtatgtt aaccaaaatc aaggctgcaa ctatcgcgac
    36481 ggaatcagga gttcctgttt atatctgctc atccttgaaa tcagattcca tgattgaggc
    36541 ggcagaggag actgaggatg gttcttactt tgttgctcaa gagaaggggc ttcgtaccca
    36601 gaaacaatgg cttgccttct atgctcagag tcaaggttct atttgggttg ataaaggggc
    36661 tgcggaagct ctctctcaac atggaaagag tcttctctta tctggtatcg ttgaagcaga
    36721 aggagccttt tcttacggtg atatcgtgac agtatttgac aaggaaagtg gaaaatcact
    36781 tggaaaagga cgcgtgcaat ttggagcatc tgctttggag gatatgttgc gttctcaaaa
    36841 agccaagggt gtcttgattt accgtgacga ctggatttcc attactcctg aaatccaact
    36901 actttttaca gaattttaga ggtaaactat ggtgagtaga caagaacaat ttgaacaggt
    36961 acaggctgtt aaaaaatcga ttaacacagc tagtgaagaa gtgaaaaacc aagccttgct
    37021 agccatggct gatcacttag tggctgctac tgaggaaatt ttagcggcta atgccctcga
    37081 tatggcagcg gctaagggga aaatctcaga tgtgatgttg gatcgtcttt atttggatgc
    37141 agatcggata gaagcgatgg caagaggaat tcgtgaagtg gttgccttac cagatccaat
    37201 cggtgaagtt ttagaaacaa gtcagcttga aaatggtttg gttatcacaa aaaaacgtgt
    37261 agctatgggt gtcatcggta ttatctatga aagccgtcca aatgtgacgt ctgatgcggc
    37321 tgctttgact cttaagagtg gaaatgcggt tgttcttcgt agtggtaagg atgcctatca
    37381 aacaacccat gccattgtca cagccttgaa gaagggcttg gagacgacta ctattcatcc
    37441 aaatgtgatt caactggtgg aggatactag ccgtgaaagt agttatgcta tgatgaaggc
    37501 caagggctat ctagaccttc tcattcctcg tggaggagct ggcttgatca atgcagtggt
    37561 tgagaatgcg attgtacctg ttatcgagac agggactggg attgtccatg tctatgtgga
    37621 taaggatgca gacgaagaca aggcgctgtc tatcatcaac aatgctaaaa ccagtcgtcc
    37681 ttctgtttgt aatgccatgg aggttctgct ggttcatgaa aacaaggcag taagcttcct
    37741 tcctcgcttg gagcaagtgt tggttgcaga tcgaaaagaa gctgggttgg aaccaattca
    37801 attccgccta gatagcaaag caagccagtt tgtttcaggt caagctgctc aagcacaaga
    37861 ctttgatacc gagtttttag actatattct agctgttaag gttgtgagca gtttagaaga
    37921 agcggttgcg catattgaat cccacagtac ccatcattcg gatgccattg tgacggaaaa
    37981 tgctgaagct gcagcatact ttacagatca agtggactct gcagcggtgt atgttaatgc
    38041 ctcaactcgt ttcacagatg gaggacaatt tggtcttggt tgtgaaatgg ggatttctac
    38101 tcagaaattg cacgcgcgtg gtcctatggg cttgaaggaa ttgactagct acaagtatgt
    38161 ggtcactggt gatggacaga taagggagta agagatgaag attggattta tcggtttggg
    38221 gaatatgggt gctagtttag ccaagtctgt cttgcaggct agaccgtcag atgagattct
    38281 ccttgccaat cgtagtcaag tcaaggtgga tgctttcatc gctaacttcg gtggtcagac
    38341 ttctaacaat gaagaaatat tcgaagaagc agatgtgatt tttctaggtg ttaagcctgc
    38401 tcagttttct gaactgcttt ctcaatacca gaccatcctt gaaaaaagag aaagtcttct
    38461 tttgatttcg atggcagctg gattgatctt agaaaaacta gcaagtctta tcccaagcca
    38521 acaccgaatt attcgtatga tgcctaatac ccctgcttct atcgggcaag gagtgattag
    38581 ttatgccttg tctcctaatt gcagggctga ggacagtgag ctcttttgtc agcttttatc
    38641 caaggctggt ctcttggttg aattaggaga aagtttaatc aatgcagcga caggtcttgc
    38701 aggctgtgga ccagcctttg tctatctctt tattgaggcc ttggcagatg caggtgttca
    38761 gacgggatta ccacgagaaa cagccttgaa aatggcagcc caaactgtgg taggagctgg
    38821 gcaattggtc ctagaaagcc agcaacatcc tggagttttg aaagatcaag tttgtagtcc
    38881 aggcggttcg actattgctg gcgtagcaag cctagaagcg catgcttttc gaggaacggt
    38941 catggaggca gttcatcaag cctataaacg aacacaagaa ctaggtaaat aagaggtagt
    39001 tttatctgcc tcttttatgg tggttgatac tcttcgaaaa tcaaattcaa actgcgtcag
    39061 cttcaccttg ccgtactcaa gtacagcctg cggctagctt cctagtttgc tctttgattt
    39121 tcattgagta tgaaatgaga agacaaaaag attgtcacaa acccctattt ttttgataga
    39181 atagaagtag taaaaaagaa atgagttaga catgtcaaaa ggatttttag tctctcttga
    39241 gggaccagag ggagcaggca agaccagtgt tttagaggct ctgctaccaa ttttagagga
    39301 aaaaggagta aaggtgctga cgacccgtga acctggcgga gtcttgattg gggagaagat
    39361 tcgggaagtg attttggatc caagtcatac tcagatggat gctaaaacag agctacttct
    39421 ctatattgcc agtcgcagac agcatttggt ggaaaaagtt cttccagccc ttgaagctgg
    39481 caagttggtc atcatggatc gttttatcga tagttctgtt gcctatcagg gatttggtcg
    39541 tggcttagat attgaagcca ttgactggct caatcagttt gcgacagatg gcctcaaacc
    39601 cgatttgaca ctctattttg acatcgaggt ggaagaaggg ctggctcgca ttgctgcgaa
    39661 tagtgaccgc gaggtcaatc gtctggactt ggaaggtttg gaattgcaca agaaagtccg
    39721 tcaaggctac ctttctctcc tggaaaaaga aggaaatcgc attgtcaaga ttgatgctag
    39781 tctccctttg gagcaagttg tggaaactac caaggctgtc ttgtttgacg gaatggactt
    39841 ggccaaatga aacaagatca actaaaggct tggcaaccag ctcagtttga ccgttttgtc
    39901 cgtatcttag aacaagacca gctcaatcac gcctatctct tttcaggttt ctttggaagc
    39961 ttggaaatgg cgcaattttt agctaagagc ctcttttgta cggataaagt tggcgtctta
    40021 ccatgtgaga aatgccgaag ttgcaagctg attgaacagg aagagtttcc agatgtcacc
    40081 ttgattaagc cagtcaatca ggtcatcaag acagaacgca ttcgggaatt ggtgggacag
    40141 ttttctcaag cagggattga aagccagcaa caggtcttta ttatcgagca agcggataaa
    40201 atgcatccca acgcagccaa ttctctgctc aaggtcatcg aagaacccca gagtgaagtt
    40261 tatattttct tcttgactag cgatgaggaa aagatgttac cgacaatccg aagtcggact
    40321 cagatcttcc actttaaaaa gcaagaagaa aaacttatct tactcttaga acaaatggga
    40381 cttgttaaga aaaaagcgac tcttttagct aagtttagtc aatcgcgagc tgaagcagaa
    40441 aagttggcac atcaggcaag tttttggacc ttggtcgatg aaagtgaacg cctgctgact
    40501 tggttagtag ctaagaaaaa agaaagttat ctacaggttg ccaaattagc caacttggca
    40561 gatgataagg aaaaacagga tcaggtttta cggattcttg aagttctctg tgggcaggac
    40621 ctcttgcagg taagagtaag agtgattcta caagatttac tagaagctag aaaaatgtgg
    40681 caagctaatg tcagctttca aaatgccatg gaatatctgg tcttgaaaga aatataaact
    40741 caaaaatgaa tgataaagaa aggaaagggc tgttttatgg acaaaaaaga attatttgac
    40801 gcgctggatg atttttccca acagttattg gtaaccttgg ccgatgtgga agccatcaag
    40861 aaaaatctca agagcctggt agaggaaaat acagctcttc gtttggaaaa ttctaagttg
    40921 cgagaacgct tgggtgaggt ggaagcagat gctcctgtca aggccaagca tgttcgtgaa
    40981 agtgtccgtc gcatttaccg tgatggattt cacgtatgta atgattttta tggacaacgt
    41041 cgagagcagg acgaggaatg tatgttttgt gacgagttgc tatacaggga gtaggcatgc
    41101 agattcaaaa aagttttaag gggcagtctc cctatggcaa gctgtatcta gtggcaacgc
    41161 cgattggcaa tctagatgat atgacttttc gtgctatcca gaccttgaaa gaagtggact
    41221 ggattgctgc tgaggatacg cgcaatacag ggcttttgct caagcatttt gacatttcca
    41281 ccaagcagat cagttttcat gagcacaatg ccaaggaaaa aattcctgat ttgattggtt
    41341 tcttgaaagc agggcaaagt attgctcagg tctctgatgc cggtttgcct agcatttcag
    41401 accctggtca tgatttagtt aaggcagcta ttgaggaaga aattgcagtt gtgacagttc
    41461 caggtgcctc tgcaggaatt tctgccttga ttgccagtgg tttagcgcca cagccacata
    41521 tcttttacgg ttttttaccg agaaaatcag gtcagcagaa gcaatttttt ggcttgaaaa
    41581 aagattatcc tgaaacacag attttttatg aatcacctca tcgtgtagca gacacgttgg
    41641 aaaatatgtt agaagtctac ggtgaccgct ccgttgtctt ggtcagggaa ttgaccaaaa
    41701 tctatgaaga ataccaacga ggtactatct ctgagttatt agaaagcatt gctgaaacgc
    41761 cactcaaggg cgaatgtctt ctcattgttg agggtgccag tcagggtgtg gaggaaaagg
    41821 acgaggaaga cttgttcgta gaaattcaaa cccgcatcca gcaaggtgtg aagaaaaacc
    41881 aagctatcaa ggaagtcgct aagatttacc agtggaataa aagtcagctc tacgctgcct
    41941 accacgactg ggaagaaaaa caataaaggg agacaggatg taataattct gtctgtttct
    42001 gtttaactta attagtgatg ataatataaa gatgtatcac ttggtataga agctttggta
    42061 ttaagttttt tattaagccc atacggaata ccgatggttg gagcagcagt tatagcgttc
    42121 ttagaaggta taaatagaaa aataaggtca ttttaaatca aaggattgat aaatcagaaa
    42181 gaaggtgatt ttttgcgaac atacgaaaat aaagaagaac taaaagctga gatagagaaa
    42241 acatttgaga aatatatttt agaatttgat aatattccag aaaatttaaa agataagaga
    42301 gctgatgaag ttgacagaac tccagcagaa aaccttgctt atcaggttgg ttggaccaac
    42361 ttggttctta aatgggaaga agatgaaaga aaggggcttc aagtaaaaac accatcggat
    42421 aaatttaaat ggaatcaact tggtgaatta tatcagtggt tcacagatac ctacgctcat
    42481 ttatctctgc aagagttgaa agcaaaatta aatgaaaata ttcattctat ctctgcaatg
    42541 attgattcgt tgagtgagga agaattattt gaaccgcata tgagaaagtg ggctgatgaa
    42601 gcgactaaaa cagcgacttg ggaagtgtat aagtttattc atgtaaatac ggttgcacct
    42661 tttggaactt tcagaactaa aatcagaaaa tggaagaaga tagtattata aattatattt
    42721 ttaactttaa aaaatttcat aaaaatggtt accaaaggcg atagaagaaa aactatcgtc
    42781 tttttctttg caaattttta agaagggagg tgatcttgca tggactttga atatttttat
    42841 aacagagaag cggaaagatt taacttctta aaagtaccgg agatattagt tgatagagaa
    42901 gaatttcggg gcttatcagc agaagcaatt atcctttatt ccatacttct taaacagaca
    42961 ggaatgtcat ttaagaataa ctggatagac aaggaaggca gagtatttat ctattttact
    43021 gtcgaagaaa ttatgaaaag aagaaatatc tcaaagccaa ctgccataaa aacattagat
    43081 gagcttgatg taaaaaaagg aataggactg atcgaaagag taaggcttgg acttggtaag
    43141 ccgaacatca tttatgttaa agactttatg agtatatttc aggtaaaaga aaatgactta
    43201 cagaagtcaa aaaacttaac ttcagaagta aaagatttta acctcagaag taaagaaaat
    43261 gaacttcaag aggttaagaa ccttgactct aactatatag agaataataa gagtaagtat
    43321 agtaagagag aatatagttt tggtgaaaac ggacttggaa catttcaaaa tgtgttttta
    43381 gctgctgaag atatatcgga tttacaaatc ataatgaact cacagcttga gaattacatt
    43441 agacttcctg caaaaataga atcctagttc atgattgata ataccagcaa tcaaattcat
    43501 tcgtaatccg aagcgtttac gatgattttg ataggttgtt gaaaacattt taaacgtttt
    43561 tactttggca aagatgttct caaccttgct tctctcctta gatagcgcat ggttacaggc
    43621 tttatcttca actgttagcg gcttgagttt gctggattta cgtg
//
LOCUS       DAVKHZ010000009        42422 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000009 DAVKHZ010000000
VERSION     DAVKHZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.009"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            161..361
                     /locus_tag="VWW99_000432"
     CDS             161..361
                     /locus_tag="VWW99_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9538321.1"
                     /translation="MSKLRGYRVMLGLTQQQMADKLKISLQSYNNKELGKTPFNDKER
                     LAIKSMVAEIKSDITIDELFYS"
     gene            376..952
                     /locus_tag="VWW99_000433"
                     /pseudo
     CDS             376..952
                     /locus_tag="VWW99_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            1386..1838
                     /locus_tag="VWW99_000434"
     CDS             1386..1838
                     /locus_tag="VWW99_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538322.1"
                     /translation="MTETTYENLTRHIDRISAEMREIGEKNGLSRLSLLANQTKFIKE
                     NLHRLLWIELPELNDSHKIEAVSKSTTGMFFHAGIFEMDAMRQAFFKRQAMHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            1835..2035
                     /locus_tag="VWW99_000435"
     CDS             1835..2035
                     /locus_tag="VWW99_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538323.1"
                     /translation="MTLDLDNMTQAEFDEVMAEIKAKRPNLFQFIADFVDRKVSTEEV
                     DEYLKMKHEKQVEFIKSYQARA"
     gene            2037..2252
                     /locus_tag="VWW99_000436"
     CDS             2037..2252
                     /locus_tag="VWW99_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538324.1"
                     /translation="MNELDLTNTQSVIFMVVLIGLLLYLNHRDRKKSTQFERENQRTI
                     ETPSEDLNPDYGRYIQLAGVNVWGGTE"
     gene            2249..2590
                     /locus_tag="VWW99_000437"
     CDS             2249..2590
                     /locus_tag="VWW99_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9538325.1"
                     /translation="MSLSDNDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRYGVPIVGVRHGTFRGYFIPANKEELLDGAKAFYNQVQEEQKRLTILLNADLESY
                     KETLKEVGGYV"
     gene            2583..2858
                     /locus_tag="VWW99_000438"
     CDS             2583..2858
                     /locus_tag="VWW99_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9538326.1"
                     /translation="MFSLSRESEQDLAQGILEVVERYLEARDRLKPRLTGLISAQEVM
                     DELDIKYMTLQKWEKAGLRRYQPPLEDTRKIYYRISDIWKFMGVENG"
     gene            2999..3865
                     /locus_tag="VWW99_000439"
     CDS             2999..3865
                     /locus_tag="VWW99_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEU9538327.1"
                     /translation="MTIYETRGFQGNLVYPFDKIEPFQYIERFKPLVVPEGANIEEFK
                     RTQAPYCISGKVTPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTSEGFIKDVSSTLFGY
                     SYILYPTIKHNLEKPRFRLVVKPDNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDLASYQKIVEHGLDYPVPQTTAEPVKQNATSLPYTPRPSGQKSMTMRIIDT
                     LFNGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTF
                     SSIARAEYRKRG"
     gene            3909..5384
                     /locus_tag="VWW99_000440"
     CDS             3909..5384
                     /locus_tag="VWW99_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEU9538328.1"
                     /translation="MEVREQEHPPRTMKELENRIFKAGEEWRAEHTETKVNETTGDVT
                     EKVAMPQTFTVAKILSEIVTFTFISKINIPDYSLLYIYDLDEGIYTASNDLFNLLCKT
                     FDVRIKPREWPQIKLMVRTLVKIRKPLESANLIPVQNGIINLETKELLPFSPKYVITS
                     KISTAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINSNHTRNKFAI
                     FYGDGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYL
                     KNPSDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVP
                     FNADFNGEKEKPWIKNEFLANDEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDN
                     DYLLSWVKHEYMEKGWHELDVVPVFILTRSLKHYAEDMGIPKPNVYGAGKETIRHLQQ
                     LTPNHYKLKRARVKVEDYDKLDPLEFERTKLSKTNHAVVKD"
     gene            5747..6277
                     /locus_tag="VWW99_000441"
     CDS             5747..6277
                     /locus_tag="VWW99_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538329.1"
                     /translation="MTLKTFSDKAKTFTFTYEFKDLDTAMVAGHALLGYMTGTYEVPS
                     ISITHKDKGTLVAEYVEDHKLNKTFKRICDSFKDYYNQLVDDEAFEERYKRERVLQLK
                     ESEDFESLLEKVTDYELELLDYADRLLSDKPIPMDSMTAFGTLEMLGDESISLIQKLD
                     VEGEYKGLAVYTEHLK"
     gene            7424..7843
                     /locus_tag="VWW99_000442"
     CDS             7424..7843
                     /locus_tag="VWW99_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9538330.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELEALEGGIIQKPTLSHSR
                     VQTSRENKTENNFISVLKLKEDTLQRIERITEERMEISGLIDKLANPLERSVLRLFYL
                     NDLDIWGVAEEIGKSKASIYRAKKAAIEHLACMVNGN"
     gene            8591..8800
                     /locus_tag="VWW99_000443"
     CDS             8591..8800
                     /locus_tag="VWW99_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538331.1"
                     /translation="MAKVKEKWNPTISHIVPKGTKLSDGTILDKETTLTQEEFTKNPP
                     VIPAGHPYYNLLARISREEIEKEDL"
     gene            9352..12183
                     /gene="uvrA"
                     /locus_tag="VWW99_000444"
     CDS             9352..12183
                     /gene="uvrA"
                     /locus_tag="VWW99_000444"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9538332.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKNVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVTNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            12176..13237
                     /locus_tag="VWW99_000445"
     CDS             12176..13237
                     /locus_tag="VWW99_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9538333.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            13369..13767
                     /gene="spx"
                     /locus_tag="VWW99_000446"
     CDS             13369..13767
                     /gene="spx"
                     /locus_tag="VWW99_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9538334.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            13833..14402
                     /locus_tag="VWW99_000447"
     CDS             13833..14402
                     /locus_tag="VWW99_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9538335.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            14489..14755
                     /locus_tag="VWW99_000448"
     CDS             14489..14755
                     /locus_tag="VWW99_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9538336.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            14759..15178
                     /gene="ruvX"
                     /locus_tag="VWW99_000449"
     CDS             14759..15178
                     /gene="ruvX"
                     /locus_tag="VWW99_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9538337.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGKFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            15194..15499
                     /locus_tag="VWW99_000450"
     CDS             15194..15499
                     /locus_tag="VWW99_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9538338.1"
                     /translation="MSHDHNHDHEERELITLVDEEGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            15960..17210
                     /locus_tag="VWW99_000451"
     CDS             15960..17210
                     /locus_tag="VWW99_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9538339.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            17207..17749
                     /locus_tag="VWW99_000452"
     CDS             17207..17749
                     /locus_tag="VWW99_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9538340.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            17895..19427
                     /gene="cls"
                     /locus_tag="VWW99_000453"
     CDS             17895..19427
                     /gene="cls"
                     /locus_tag="VWW99_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9538341.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            19505..19648
                     /locus_tag="VWW99_000454"
     CDS             19505..19648
                     /locus_tag="VWW99_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538342.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            19685..21223
                     /locus_tag="VWW99_000455"
     CDS             19685..21223
                     /locus_tag="VWW99_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9538343.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVGLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVEVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            21343..23550
                     /gene="nrdD"
                     /locus_tag="VWW99_000456"
     CDS             21343..23550
                     /gene="nrdD"
                     /locus_tag="VWW99_000456"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9538344.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            23562..23702
                     /locus_tag="VWW99_000457"
     CDS             23562..23702
                     /locus_tag="VWW99_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538345.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            23746..24252
                     /locus_tag="VWW99_000458"
     CDS             23746..24252
                     /locus_tag="VWW99_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9538346.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            24245..24835
                     /gene="nrdG"
                     /locus_tag="VWW99_000459"
     CDS             24245..24835
                     /gene="nrdG"
                     /locus_tag="VWW99_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9538347.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            24832..25449
                     /locus_tag="VWW99_000460"
     CDS             24832..25449
                     /locus_tag="VWW99_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9538348.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGVKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            25715..26023
                     /gene="rpsJ"
                     /locus_tag="VWW99_000461"
     CDS             25715..26023
                     /gene="rpsJ"
                     /locus_tag="VWW99_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9538349.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            26240..26866
                     /gene="rplC"
                     /locus_tag="VWW99_000462"
     CDS             26240..26866
                     /gene="rplC"
                     /locus_tag="VWW99_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9538350.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            26891..27514
                     /gene="rplD"
                     /locus_tag="VWW99_000463"
     CDS             26891..27514
                     /gene="rplD"
                     /locus_tag="VWW99_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9538351.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            27514..27810
                     /locus_tag="VWW99_000464"
     CDS             27514..27810
                     /locus_tag="VWW99_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9538352.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            27828..28661
                     /gene="rplB"
                     /locus_tag="VWW99_000465"
     CDS             27828..28661
                     /gene="rplB"
                     /locus_tag="VWW99_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9538353.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            28765..29046
                     /gene="rpsS"
                     /locus_tag="VWW99_000466"
     CDS             28765..29046
                     /gene="rpsS"
                     /locus_tag="VWW99_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9538354.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            29058..29402
                     /gene="rplV"
                     /locus_tag="VWW99_000467"
     CDS             29058..29402
                     /gene="rplV"
                     /locus_tag="VWW99_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9538355.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            29415..30068
                     /gene="rpsC"
                     /locus_tag="VWW99_000468"
     CDS             29415..30068
                     /gene="rpsC"
                     /locus_tag="VWW99_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9538356.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            30072..30485
                     /gene="rplP"
                     /locus_tag="VWW99_000469"
     CDS             30072..30485
                     /gene="rplP"
                     /locus_tag="VWW99_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9538357.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            30495..30701
                     /locus_tag="VWW99_000470"
     CDS             30495..30701
                     /locus_tag="VWW99_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9538358.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            30726..30986
                     /gene="rpsQ"
                     /locus_tag="VWW99_000471"
     CDS             30726..30986
                     /gene="rpsQ"
                     /locus_tag="VWW99_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9538359.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            31012..31380
                     /gene="rplN"
                     /locus_tag="VWW99_000472"
     CDS             31012..31380
                     /gene="rplN"
                     /locus_tag="VWW99_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9538360.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            31458..31763
                     /gene="rplX"
                     /locus_tag="VWW99_000473"
     CDS             31458..31763
                     /gene="rplX"
                     /locus_tag="VWW99_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9538361.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            31787..32329
                     /gene="rplE"
                     /locus_tag="VWW99_000474"
     CDS             31787..32329
                     /gene="rplE"
                     /locus_tag="VWW99_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9538362.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            32347..32616
                     /gene="rpsN"
                     /locus_tag="VWW99_000475"
     CDS             32347..32616
                     /gene="rpsN"
                     /locus_tag="VWW99_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9538363.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            32901..33299
                     /gene="rpsH"
                     /locus_tag="VWW99_000476"
     CDS             32901..33299
                     /gene="rpsH"
                     /locus_tag="VWW99_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9538364.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            33491..34027
                     /gene="rplF"
                     /locus_tag="VWW99_000477"
     CDS             33491..34027
                     /gene="rplF"
                     /locus_tag="VWW99_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9538365.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            34111..34467
                     /gene="rplR"
                     /locus_tag="VWW99_000478"
     CDS             34111..34467
                     /gene="rplR"
                     /locus_tag="VWW99_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9538366.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            34485..34979
                     /gene="rpsE"
                     /locus_tag="VWW99_000479"
     CDS             34485..34979
                     /gene="rpsE"
                     /locus_tag="VWW99_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9538367.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            34951..35175
                     /gene="rpmD"
                     /locus_tag="VWW99_000480"
     CDS             34951..35175
                     /gene="rpmD"
                     /locus_tag="VWW99_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9538368.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            35320..35760
                     /gene="rplO"
                     /locus_tag="VWW99_000481"
     CDS             35320..35760
                     /gene="rplO"
                     /locus_tag="VWW99_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9538369.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            35773..37083
                     /gene="secY"
                     /locus_tag="VWW99_000482"
     CDS             35773..37083
                     /gene="secY"
                     /locus_tag="VWW99_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9538370.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            37234..37872
                     /locus_tag="VWW99_000483"
     CDS             37234..37872
                     /locus_tag="VWW99_000483"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9538371.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAQALDKTLA
                     ELGIELEGVINIEVNPNSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            37989..38207
                     /gene="infA"
                     /locus_tag="VWW99_000484"
     CDS             37989..38207
                     /gene="infA"
                     /locus_tag="VWW99_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9538372.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            38232..38348
                     /gene="rpmJ"
                     /locus_tag="VWW99_000485"
     CDS             38232..38348
                     /gene="rpmJ"
                     /locus_tag="VWW99_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9538373.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            38366..38731
                     /gene="rpsM"
                     /locus_tag="VWW99_000486"
     CDS             38366..38731
                     /gene="rpsM"
                     /locus_tag="VWW99_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9538374.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            38749..39132
                     /gene="rpsK"
                     /locus_tag="VWW99_000487"
     CDS             38749..39132
                     /gene="rpsK"
                     /locus_tag="VWW99_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9538375.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39175..40110
                     /locus_tag="VWW99_000488"
     CDS             39175..40110
                     /locus_tag="VWW99_000488"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9538376.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            40122..40508
                     /gene="rplQ"
                     /locus_tag="VWW99_000489"
     CDS             40122..40508
                     /gene="rplQ"
                     /locus_tag="VWW99_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9538377.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            40773..41039
                     /locus_tag="VWW99_000490"
     CDS             40773..41039
                     /locus_tag="VWW99_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9538378.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            41049..42386
                     /locus_tag="VWW99_000491"
     CDS             41049..42386
                     /locus_tag="VWW99_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9538379.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        13447 a         7913 c         9474 g        11588 t
ORIGIN      
        1 aaagtacatt ttttttgtgc aaaaattaaa aaaatagttg acaatagaaa aaatattgtg
       61 tatcatacaa ttcaagcaca gaaaaaatgt gcaaactcct ccgacctttc acgctttcaa
      121 tctattcatg gagggggatt ttttagaaag ggggaaagct atgagtaaac tacgaggcta
      181 tcgagttatg ttaggactaa ctcagcaaca aatggcggac aagctaaaaa tttctttgca
      241 gtcatacaac aacaaagaat taggtaaaac gccattcaat gacaaagaac gcctagcgat
      301 taagtcaatg gttgcagaaa tcaaatcaga tataaccata gatgaactat tttatagcta
      361 gaaaggagca aaccgatgga actagtctat atggacggta aaaaagagcc ttatacgctg
      421 agcagtattg tggcagaatg cacgggattg caacatcaca caataaccaa gacgatccgc
      481 aaacatcaag taaggtttga acagttcgga aaggttgaat ttaaaatcca agctatggaa
      541 agtggccaga ataccaagga ctatattttg aacgaacaac aagcgacctt gttagttaca
      601 ttcttaaaga acaccgagca agtggccaac ttcaaaacca acctggtcaa agctttcttt
      661 gaaatgcgtg aggaactttc taagtttcgt atgcagaggg cgctagaaaa gccaaaaaga
      721 aaaacactgc atgaacaacc tgctgctaaa agcagtaact gacatgaacg ctaagcagtt
      781 aagggaagaa cgtgggggct ataacggtat tgatagcttg accagtgacg agctggagca
      841 atatcaggca tttgaggata tggtaatagc catgattggc ttgaatatga gttatcagga
      901 aatcaaagct atggcattca gaaataaaaa aacacgccaa agaagcgcgt gagagcaaca
      961 aaaaaggctt accgagacca atcagcagag cctttcacac taacactaaa acgaatttaa
     1021 caaagcaggc aagctattat caaagaggtt ttagtaaaga tttataccta gattatagca
     1081 tatttagggc aatttgacca tacggagagc gccaactctt taaactggta ctttctcacg
     1141 ctttcaattt ggcgactctg agcgtgagga ttagcgttat aagaaacaac tcaaaaaagc
     1201 cccacgctca aagattggac ccagagagcg tgaggctgta ggcaagaaaa actgtataag
     1261 aaacaaccat tcaaaatccc cacactcaaa ttttggtcga ggagagtgtg aggatatcca
     1321 gtatagtaaa aggcattaaa aagcccttag ggcaattaca caacatagaa acaaaggtga
     1381 aaaacatgac agaaacaaca tacgagaatt taactagaca tatagacaga attagcgcag
     1441 aaatgcgaga aattggcgaa aaaaatgggc ttagtagact ttcattattg gcaaatcaga
     1501 caaaattcat caaagaaaat ttacaccgtt tactttggat tgaacttcct gaattgaatg
     1561 acagtcataa aatcgaggca gtttctaaaa gcactacggg aatgtttttc cacgctggta
     1621 tttttgaaat ggacgctatg cgacaagcat tctttaaacg ccaagctatg cacttttttg
     1681 acaacgaagc agagcaacag gcgtatatag aacatgctga aaaggagtat ttagaggcta
     1741 ctataacctt aaaagatatt ctttttaact ctaaaaatgg aactcagaga gtaaataaaa
     1801 gttgtcttat agagaagttt gaggaggcaa tgcaatgaca ctagacctag acaacatgac
     1861 acaagcagaa tttgatgaag taatggctga aatcaaggcg aaacgcccaa acctcttcca
     1921 gtttattgcg gattttgtag atcggaaagt aagcactgag gaggtagacg aatacctgaa
     1981 gatgaaacat gaaaaacaag tggaatttat caagagttat caagcgaggg cataacatga
     2041 atgagttaga tttgaccaat acacaatcgg taatctttat ggtggtattg attggcttac
     2101 tgctttattt aaaccaccga gaccgcaaaa aaagcaccca atttgagcga gaaaaccaac
     2161 ggacgataga aacacctagc gaggatttaa accctgatta tgggcgttat atccagcttg
     2221 caggggtcaa cgtatgggga ggaactgaat gagtctatca gataatgaca aacgagtatt
     2281 gagactaatc aaggtaggtg ctgagaactc cataacaggg gcagaaatca gcctgatcac
     2341 taagctggca gaaagaaccg tgcaagatat tatcagccgt ctaatcacgc gctatggcgt
     2401 tccaatcgtt ggggttcgac acggaacatt tagaggatat tttatcccag ctaacaaaga
     2461 ggagttactg gacggagcaa aggcattcta taaccaagta caggaagaac agaaacgcct
     2521 aactatcctt ttaaatgctg atttagagag ttacaaagaa accttgaagg aggtgggcgg
     2581 atatgtttag cctaagccga gaaagcgagc aagacctagc gcaaggcatt ctggaggtag
     2641 tggaaagata cctggaagcg cgtgatagac tcaagccacg gctgacaggt ttaatctcag
     2701 cccaggaagt catggatgag ttagatatta agtatatgac gctccaaaaa tgggaaaaag
     2761 cagggctaag gcgataccag cccccactag aagatactag gaaaatctat tatagaattt
     2821 cggacatctg gaagtttatg ggggtggaga atggctagat ataacacaca cccagcacaa
     2881 ggcggaggtc attatcatga cattaaattg tataagcacc gtggcagatc tttggaacag
     2941 ttccaagaac aaaagctact gaagaagtta aaaaagaaac gcaaaaaggg gcgatagtat
     3001 gacaatttac gagactagag gctttcaggg taatcttgtc tatccatttg ataagattga
     3061 gccgttccag tacattgaac gctttaagcc tttggtagtc cctgaagggg caaacattga
     3121 agaatttaaa cgtacacaag cgccttactg tatcagtggg aaagtgacac cagaaaagaa
     3181 tggcagttac aagcgaaaca actccagtct aatctatcgg gatttgattt tccttgatta
     3241 tgacgatata caggggacgt ctgagggctt tataaaggac gtttctagca ccttgtttgg
     3301 ctactcatat attttatacc cgactattaa acataactta gaaaagcccc gttttcgcct
     3361 tgtagtgaag cctgacaacg tgatgaatga ggcaacctat aagcaggtag tgaaagagat
     3421 tgctgacaag attggactac cctttgatat ggccagcctt acatggtcac aacttcaagg
     3481 gctaccagta acaacaggcg acctagcaag ttatcaaaag attgtggagc atggacttga
     3541 ttatccagta ccacagacaa cggctgaacc agtcaaacag aatgctacaa gtcttccata
     3601 cactccaagg ccaagcggtc agaaatctat gaccatgagg atcattgaca cgcttttcaa
     3661 cggctttgga gacgagggag ggcgcaacgt agcattgaca cgctttgtag gcttactctt
     3721 taataagtgg gttgattgtg acctagagac cgcctatgaa cttacaaaga tagccaatag
     3781 cgtgacggtt gaaccattgc ctattgaaga gctagaccgc acttttagca gtatagcacg
     3841 ggctgaatat agaaagagag gttagaacca tagaactaga agaattagca aacctaaaaa
     3901 gtgaaatcat ggaggtcaga gagcaagaac accctcctag aacgatgaag gagcttgaaa
     3961 atcgtatctt taaagctggt gaagaatggc gggcagaaca taccgaaact aaagtaaatg
     4021 agaccacagg ggacgtcacc gaaaaggtgg ccatgcccca aacattcaca gtagctaaaa
     4081 tactgagcga gattgtcaca tttactttta tcagcaagat caatatacct gattatagcc
     4141 tgctctatat ctacgattta gacgagggta tctataccgc tagtaatgat ctattcaatc
     4201 ttttgtgtaa gacctttgac gttagaatta aacccagaga gtggccacag attaagttaa
     4261 tggttagaac attggtaaag atacgaaaac ctttagaaag tgctaacctc attcccgtac
     4321 agaacggcat tatcaactta gaaactaagg aactactccc ctttagtcct aagtatgtaa
     4381 ttacaagtaa gataagcacg gcctaccacg cgcctaaacg agtcccaaca gatagagagg
     4441 gcaaaacttt tgatgattgg ctaaactcaa tcgcttgtaa tgatagtgaa ctggtaacac
     4501 tgttttggca gattatcctt gaagctatta actctaacca tacccgaaat aagtttgcta
     4561 tcttctacgg ggacggtaac aacggtaagg gaacatttca gcgcttcctt atcaacctga
     4621 taggggaaag caatatatca gccttaaaac ctgcacagtt tgctgaaaag cataacttag
     4681 aaaccctggt aggtaaagtt tgtaatatcg gagatgaagc cccaaacgaa tacttaaaaa
     4741 atccgtctga tttaatgagt attactagtg gcgacactgt tctagtcaac cccaaaggtc
     4801 gcccagcctt tgaagctact ttcaagttgt tcaatatctt ttcagggaat tatatcccca
     4861 atggtggcaa caagacaaaa ggctggtatc gtagaattat gatcgtccct tttaatgctg
     4921 actttaacgg ggagaaggaa aaaccttgga taaaaaatga atttttggcc aatgatgaag
     4981 tattagagta tgcactttac aaagctatca atcaagagcc atttactcac tttatcgaac
     5041 ctaaagcagt taaaggactc ctagaagaat accaggagga taatgactac ttactttcat
     5101 gggttaagca tgaatacatg gaaaaaggtt ggcatgagct ggacgtagta cctgttttta
     5161 tactaactag gtcgctgaaa cattatgctg aagatatggg aatacccaag cccaatgttt
     5221 atggggcagg aaaagaaacg ataagacact tacaacagtt aacacccaat cattacaaac
     5281 ttaaaagagc gagggtcaaa gttgaagatt atgacaaact agatccgcta gagtttgaaa
     5341 gaaccaaact atccaaaact aatcacgctg tcgttaaaga ctaaaatgtt accttctttt
     5401 tatgccctaa acccttggta tatctaggtt tttacccaaa aatgttacct tcttgttacc
     5461 ttgtttagaa agaaggtaac gttgttaaac ccttgatact actgactttt ttgggaaaat
     5521 gttaccttgt taccttgttt ttaactctct atatagggaa aaatagtatt tttatatata
     5581 agggctatga cttcaaaaga aggtaacatg gtaacaaaag ggtctaaacc cttgggggtg
     5641 taaggcttaa ctatgttacc tagaaggtaa caaaatcact aaagaaggta acaaattaac
     5701 gaatattaca taatttacct taaactaaga aacggagaaa aacaacatga cattaaaaac
     5761 attttcagat aaagccaaaa catttacttt cacttacgaa tttaaagacc tagacactgc
     5821 tatggtagca ggacacgcgc tactgggata tatgactggg acttatgaag taccatctat
     5881 ttcaataact cacaaggata aagggacgct tgtagctgag tatgtggaag atcacaaact
     5941 aaacaagacc ttcaagcgta tttgtgacag ttttaaagac tactacaacc aactagtgga
     6001 cgatgaagca tttgaagaac gttataaacg ggagcgcgtg ctccaactca aagagtcaga
     6061 ggactttgag agtttgctgg agaaagtaac agactacgag ctggaactgt tagactatgc
     6121 tgatcgcttg ctttctgata agcctatccc tatggactca atgaccgctt ttggtacgtt
     6181 ggaaatgctg ggcgatgaaa gtattagcct aatccaaaag ttggacgtag agggcgaata
     6241 taaaggcctt gctgtttata cagagcattt gaagtagaaa acaggggagc aatccccttt
     6301 tagtatgtaa cccaatcgat ttaggttttt tacatgataa tttgtagtag tgtgcaattt
     6361 acaaaacaaa caaatactac atattagacg gtttgaatgt taaaaaagct agatatatca
     6421 agggtttaag taagtagagc gttccaaaaa agggtgcggt aatcgttcca aaaaaagggt
     6481 gaggtaaggg tgaggttaca tatatataaa gtacaaggtt agtacaaggt tgtgtattta
     6541 aaaatattaa aaataggcta agattaggct aatgtcacat acttcaaaat actaaggttt
     6601 tagcaaggta cagggctttt tttctagtga tatgacccta agattaccct aaggtatagc
     6661 tactttttta gaaccataga acggtaaggt tttggtaagg tatcgaagtt ttccaatggt
     6721 gaggttttgg tgaggtaaga gcaaaaaatg taaaggtttt gtaaaggttg cgtatttgaa
     6781 agccccaagg ttagcacaag gtagagaggt ttaccgaggg ttagaaaagg tgagattttg
     6841 cttaggtagt ggcagtaaat atgcctgaca aatggcaggg ttatgtgtgt tgaactccga
     6901 gcgaacgccg agaaagtgga tatatcccct atcatttatc aagcagataa gagaaataat
     6961 aaccctcctt aactcccgga gaaacgacaa gcagataagc ggagttttta cccctcttat
     7021 ttccgattta ctccttgcag aatgataggc agataaggac gacaataagc cttcttaatt
     7081 gcttacaaaa cgatagggag ttaagacgaa tttaaaagct ctttattttc gtttttctcc
     7141 ttacaaaatg acagggaaat aagaactaaa ataactctcc ttattttcct accaaatgat
     7201 aaggagataa gagcaattta aaagtctgtt attttggatt tgttgcttac caaatgataa
     7261 gcagatcagc aaaggaataa cttgccttaa ttgcttatca aatggtacgt agaatggact
     7321 tgaacctaga gcgaagctag agaaaatgaa aaaaagccag cacacggctg actcctttat
     7381 gaattattcc ctaaaactat tatatcacaa aggaggccga ggcatgacac ccgagcaggt
     7441 aaaagaaaaa ctagagggcg ttaagtggat aaataaggag atcaaaggct tatatttgga
     7501 attggaagcc ctggaaggtg gcattatcca aaagccaaca ctaagccata gcagggtgca
     7561 gacaagcaga gagaacaaga cagagaacaa ttttataagt gttctgaagc taaaagagga
     7621 tacgctccag aggattgagc gcattactga ggaaagaatg gaaatatctg ggctgatcga
     7681 taagctagcc aatccgcttg agcgctctgt tctgagactt ttttacttga atgatctcga
     7741 tatttgggga gttgctgagg aaataggtaa atctaaagct tcgatatatc gggcaaagaa
     7801 ggcagctata gaacacttgg catgtatggt aaatgggaat tgatttaacc ttaccacaga
     7861 gcctagtgcg tatagggggc actaggcttt atattcgccc cttattcgtt ttgttttgga
     7921 ctggataact aaatcaccac ggataaacta caagccctca gaaacaaatc taggggcttt
     7981 tgtgagataa aaaaataaag gtgacccacc gcctccacag caagctgctt catgggcgtt
     8041 agaccacctt taccttaact taattataaa tcacaatgcg gagtttttca agaaaggata
     8101 agagactaga aaactagcta cttattggaa caacaaagct agtgagctat tgaagaacgg
     8161 taagattttg gttagataca gagaaacagg cctaagcctg ctctaaatct aacttaattt
     8221 cttcacaaac tattaaattc aaataagctg agaaaacata aagggtaaca aaaaggggac
     8281 ttaaattgaa gagttcagta agcaagaaac taacacaatc aaacgtgctt tttttgtaaa
     8341 aatgttgtat acttaagtaa ataaaggaac tggtttggca cccaatggct tattttaggt
     8401 cggcgcctaa gtcagttcct ttttactgtt tcgaccaccc caaaggatat gcctctaaca
     8461 ttattgcgtc aattagttat ttaggctata attaaattaa cgaatagagg ttttccatct
     8521 ccccttgaaa cagttcattc cgcggtagga ggtgggacgc ctctattttt accataaagg
     8581 agtatttaac atggcaaaag ttaaagaaaa atggaaccca acaatatccc atattgtccc
     8641 taaaggtacc aaactatcag acggtacaat attagacaaa gaaaccacct taacacaaga
     8701 agaatttact aaaaatcccc ctgttattcc agcaggacac ccttattata atcttttagc
     8761 tagaataagc agagaggaaa tagaaaagga agatctatag tacaaagaaa tagtaaaata
     8821 taatagtttt gcgcgtgatg ttcgcttgaa aaggacttgt taaggtcaac aaaatcgata
     8881 accacgcgct tttatctatc tcaccttttc gatagataaa acaaccaata cgcgtgatga
     8941 acatggtata atatattcac agctggaggc ttgcacagtc tagccgatta gggagagtta
     9001 gaccgagatg tcacacactc ccttttttgg tataataaac ctatcagata agcaaaaaca
     9061 actaaaaaag cgtagctgat gactacgcta gtttcttgcc tgctgaactc gtcaaatctt
     9121 agcccttttg tggggctttt ttgctcccct ttttgcgtac tttgggggag caagtagtaa
     9181 aaagtaatat tagtgttttt tgttaaaaag cgcgtgatat aagggttagg aagccctaag
     9241 gaaacataaa ataagaagtt ttttattata ctacaaaatc ggccttttgg gtaatagaat
     9301 ctcacttttt ttggtataat ggtaagcaat aatggactag aaagaacaaa gatgcaagat
     9361 aaaattgtca ttcatggggc gcgtgcccat aatttaaaaa atattgatgt ggagattccg
     9421 cgagacaagt tggttgtcgt gactggcttg tcaggttcag ggaagtccag tctggctttt
     9481 gataccctct atgcggaggg acaacgtcgc tatgtagaga gtttgtccgc ctacgctcgt
     9541 cagttcttgg gaaatatgga aaaaccagat gtagatgcca ttgatggtct cagcccagct
     9601 atttccatcg accagaaaac gactagtaaa aatcctcgct cgacggtggg aacgacgact
     9661 gaaatcaatg actatctgcg tctcctctac gcacgtgtgg ggacgcctta ctgtatcaac
     9721 ggacatggag ctatcaatgc ttcttctgtg gagcaaatcg ttgataaggt tttggagtta
     9781 cctgaacgcc agcgcttgca gatcttggct cctgtcatcc gcaagaaaaa aggccaacat
     9841 aagaatgtta tcgagaaggt tcagaaagac ggatatgttc gtgtccgtgt ggatggggaa
     9901 gtctatgatg tgaccgaagt gccagagttg tctaagagca agcaacacaa tatcgatgtc
     9961 gtggttgacc gtattgttat caaggagggc attcgtagcc gtctctttga ttccattgaa
    10021 gctgcccttc gtatcgcaga aggttatgtg attatcgaca ccatggacga ttcggagttg
    10081 ctcttctctg agcattatgc ctgtccagtt tgtggattta ctgtcccaga gttagagcct
    10141 cgtctcttct ctttcaatgc tccctttggc tcttgtagtg agtgtgacgg cttggggatt
    10201 aagctggagg tggatactga tttggtagtg ccagatgcca gcaaaacctt acgtgaagga
    10261 gcgctagcac cttggaatcc tatctcatcc aactactatc caaacatgtt agagcaggcc
    10321 atgaaagtct ttggagtggc tatggataag ccttttgagg acctgtcaga agaagataag
    10381 aacttgattc tctatgggtc agatggtaag gaattccatt tccactatga gaatgaattt
    10441 ggtggcgtgc gcgatatcga cattccgttt gagggagtcg tcactaatat caaacgtcgt
    10501 taccatgaaa ccaatagcga ttacactcgc actcagatgc gtctctacat gaatgagctg
    10561 acctgcggaa cctgtcaagg ctatcgtctc aatgaccagg ccttgtctgt ccgtgtgggc
    10621 ggccagcaag ggccacatat tggagaaatc tcagacctgt ctatcgctga ccacttggac
    10681 ttggtgagcc agttaacttt gtctgaaaat gaagccatca ttgctcgtcc cattctcaag
    10741 gaaatcaagg atcgtttgac cttccttaat aatgtgggtc ttaactatct aaccctgtca
    10801 cgttcagcag gaaccctttc aggtggggaa agtcagcgca ttcgtttggc aacccagatt
    10861 ggttccaacc tatcaggcgt cctctatatc ctagacgagc cgtcaatcgg tcttcaccag
    10921 agggacaatg accgcctgat tgccagtctg aaaaagatgc gtgacttggg caatactctt
    10981 atcgtggtgg aacacgacga agataccatg cgtgaagctg attatctgat tgacgttggt
    11041 cctggtgccg gtgtttttgg tggtgagatt gttgcagcag gtacacctaa gcaagtggct
    11101 cgcaacagca agtctatcac aggtcagtac ttgtcaggca aacgtgtcat cccagtacca
    11161 gaagagcgcc gtgtcggaaa tggtcgtttt attgaagtga taggagcgcg tgagaacaac
    11221 ttgcaaaatg tcacggctcg ctttccacta ggaaaattta tcgcagtgac aggtgtgtcg
    11281 ggttcaggga aatcgactct aatcaacagc attctcaaaa aagccattgc ccagaagctc
    11341 aaccgtaatt cagacaaacc tggtaagttt aagactatta cagggattga gcatgtcgac
    11401 cgtttgattg atattgacca gagccctatc ggacggacgc cgaggtctaa cccagctacc
    11461 tatacaggag tttttgacga tatacgtgac ctttttgctc agacaaatga agccaagatt
    11521 cgaggctaca agaaggggcg cttcagtttc aacgtcaagg gaggtcgctg tgaagcttgc
    11581 tcaggtgacg ggattatcaa gattgagatg cacttcttgc cagatgttta tgtggcttgt
    11641 gaagtttgcc acggaacccg ctataacagt gaaaccctag aagttcacta caaggaaaag
    11701 aatatttcgc aggtcttgga tatgacggtc aacgatgcgg tagaattttt ccaacacatt
    11761 ccgaaaattc aacgcaaact ccagaccatc aaggatgtag gtctaggcta tgtgaccttg
    11821 ggacagccag ctaccaccct ttctggggga gaagcccaac gtatgaagtt agctagcgaa
    11881 ctccacaaac gctcgacagg aaaatctttc tacattctgg atgagccgac gacagggctt
    11941 catacagagg acattgctcg cctgctcaag gttttagctc gctttgtaga cgccggcaat
    12001 acagtcctcg tcatcgagca caatctggat gttatcaaga cggcagacca tatcattgac
    12061 ttgggacctg agggcggtgt cggtggtgga accatcatcg taacaggaac tccagaagaa
    12121 gtagctgcca acgaagccag ctatacagga cactatttga aaggaaagtt acatcatgaa
    12181 taaacgcgta caagcatttc tagctaaaat gcaagaaaaa gaactagatg gtatcatcat
    12241 caacaatctt aaaaacgtct attatttgac tggtttttgg ggctcaaacg gaacagtctt
    12301 tatcagtcgt gaccgtcagg tcttagtgac agactctcgc tatatcatcg cagctaagca
    12361 agaaaccagt ggttttgaga ttgtggctga tcgtgatgaa ttggctgtca ttgcaggaat
    12421 tgttaaggac atgggcttga ctcgtatcgg ttttgaagat gagatttcag tgtcttatta
    12481 ccaccgtatg caggcagctt ttgcaggttt gaacttgctt ccacaaactc agtttgtgga
    12541 aggtcttcgt atgattaagg atgaggcaga gattgcagcg attcgcaagg cttgttctat
    12601 ctcagaccaa gctttccgcg atgcgcttga ctttatcaaa ccaggaaaaa ctgaaattga
    12661 gattgccaac ttccttgact tccgcatgcg tgagttggga gcatctggct tatcttttga
    12721 tacgatccta gctagcggta tcaattcttc taaaccccat gcccatccaa tgcacaaacc
    12781 agtggagttg ggagaagcca ttaccatgga cttcggctgt ctctatgacc actatgtcag
    12841 tgatatgacc cggactatct atctagggca tgttagcgat gagcaggcag agatttacaa
    12901 tacggttcta aaagctaacc aagccttgat tgaccaagct aaggcaggct taggtttccg
    12961 tgactttgac aaaatccctc gtgatattat cattgaggca ggttatggcg actactttac
    13021 tcacggcatt ggccacggta ttggtctgga tatccatgag gaaccctact ttagccagac
    13081 ttctacagaa actattaaga caggtatggc cttgaccgat gaaccaggta tctatatcga
    13141 aggcaaatat ggcgttcgta tcgaggatga tatcctgatt acagagacag gttgtgaatt
    13201 attgacccta gctccaaaag agttgatagt catttagttg tttgtcaaga aaaaagtacg
    13261 aaaatgacga aaaaagtcaa aaaaaattaa aaataggtcg caagtcggat gttttttatg
    13321 gtataataga ctaaactgat agtaacatgt agcgaaaggg gtaggtacat gattaaaatt
    13381 tatacagtct caagttgtac tagctgtaaa aaagcaaaaa cctggctcaa tgcccaccag
    13441 ttaagttata aagaacaaaa ccttggtaaa gaaggaatta cgagagaaga attactggat
    13501 attctaacca aaacagataa cggaatagcc agcatcgttt cgtctaaaaa tcgctatgcc
    13561 aaagcccttg gagtggatat tgaagatttg agtgtcaatg aagttctcaa tctgattatg
    13621 gaaacaccga gaattttaaa gagcccaatc cttgtagatg aaaaacgcct gcaagttggc
    13681 tacaaggaag acgatattcg tgccttccta ccacgctctg tccgtaatgt agaaaatgca
    13741 gaagcacgtt tgcgtgcagc tctataaaca tcaaggctgg gagcacttcc cagtcttatt
    13801 ctattttaat ttcaaaaaga aagaagaaag aaatgaaaaa aatagttctt gttagtctag
    13861 ctttcctttt tgtcctggtt ggttgcggac agaaaaaaga aactggacca gctacaaaaa
    13921 cagaaaaaga tacgcttcag tcggcattgc cagttattga aaatgctgag aagaatacag
    13981 ttgtaactaa gactttggtc ttgcccaagt cagatgatgg tagccagcag acacaaacaa
    14041 ttacttacaa agacaagact tttttgagtc tagctatcca acaaaaacgt ccagtctctg
    14101 atgagttgaa gacttatatt gaccaacatg gagtggagga aactcaaaaa gctcttcttg
    14161 aagcggagga gaaggataag tctatcattg aagctcgtaa attggcaggt ttcaaacttg
    14221 aaacaaaact attgagcgca acggaacttc aaacaacgac tagttttgat tttcaagttc
    14281 tggatgtcaa gaaggcttcc cagttggaac atctgaagaa tattggtttg gaaaatcttt
    14341 tgaaaaatga accaagcaaa tatatttcag atagattggc aaatggcgcg acagaacaat
    14401 agaaaatcca aataaataga ctgcctgaat tgtagaaggt aaggaattat gctataatgg
    14461 tactattcta aggaaagagg gtgttagaat gggatttact gaagaaacag tacgttttaa
    14521 attggacgat tccaataaaa aagaaattag cgaaactttg acagatgttt atgcttcgtt
    14581 gaacgataag ggttacaacc caattaacca aatcgtaggt tacgtattga gtggagaccc
    14641 tgcctacgtt cctcgttata ataatgcacg aaatcaaatc cgtaagtatg agcgtgatga
    14701 aatcgttgag gaattggttc gctactacct caaaggacaa ggagtcgatc tataacctat
    14761 gagaattatg ggattggacg tcggttcaaa aacggtaggg gtggcgatta gcgatccgct
    14821 tggttttaca gctcaagggc ttgaaatcat ccagataaat gaagaacaag gcaaatttgg
    14881 ttttgaccgc gttaaggaat tggttgatac ttacaaggtg gaacgatttg tagtgggctt
    14941 gcctaaaaac atgaacaata caagtggacc gcgcgtagaa gctagtcaag cctacggagc
    15001 aaagctagaa gagttttttg gtttaccagt agactatcag gatgaacgct tgacaacagt
    15061 ggctgctgag cgcatgttga ttgaacaagc agatatcagt cgcaataagc gcaagaaagt
    15121 cattgataag ttagcagctc agctgatttt acaaaattat ttagatagaa aattttaata
    15181 taaaggagag gctatgtcac acgatcataa ccacgaccac gaagaacgtg aactaatcac
    15241 actagtagat gaggaaggaa atgaaacctt gtttgaaatc cttttgacaa ttgatggaaa
    15301 agaagaattt ggtaaaaact atgttcttct agtaccagtt aacgcagaag aagacgaaga
    15361 cggacaagtt gaaatccaag cttactcatt catcgaaaac gaagatggaa cagaaggcga
    15421 attgcaacca atcccagaag actcagaaga cgaatggaac atgattgaag aagtcttcaa
    15481 cagctttatg gaggagtgat acagtctggg agactgtatc agcccaactc ccagaaatgg
    15541 ggaagagttg gaacgtccag tggatgtttt tagcccagtt cctttaaata agagagaact
    15601 ggtaggttcg gggggacgtt tttagcccag ctcctttaaa taagatagag ctggtaagtc
    15661 tgggagactg tatcagccca actccagaaa tggggaagag ttggaacatc cagtggatgt
    15721 ttttagccca gttcctttaa ataagagaga actggtaggt tcgggggacg tttttagcct
    15781 gacgctaaaa ataaaaaccg tcagtaagtc cggggggcgt ttttagccca cgttcacatt
    15841 catagtagct agttattagc taaccaggta agaggtcggg acgaaaatcc tggccttgtt
    15901 tttattagta accatacttt aatgcgataa ttgattggac ttttgtcaag gagatgtgta
    15961 tgtttgaagt agaagaatgg cttcatagtc ggattggttt gaattttcga tcaggtttgg
    16021 gccgaataca gcaagcggtg gatttgttag gaaatcctga gcagtcttac cctattatcc
    16081 acgtaacagg gactaatggg aaaggatcta ccattgcttt tatgagggaa ttatttatgg
    16141 ggcatggcaa aaaagttgcg acctttacct cccctcatat cgtatccatc aatgaccgaa
    16201 tttgcattaa tgggcaacct atagcagacg cagactttat ccgtttggct gatcaggtaa
    16261 aggagatgga gaaaacgctt ctgcaaactc ctgaccagtt gtcctttttt gaattgctga
    16321 ccttggttgc ttttctttat tttagggagc aggaggtgga tttggtttta ttagaagtgg
    16381 gaattggtgg cttacttgac acgaccaatg tggtaactgg agagcttgct gtcatcacct
    16441 ccattgggct tgaccatcaa gaaaccttgg gtgatagtct agaagcaatt gcagagcaga
    16501 aagctggtat tttcaaggct ggtaaaaagg cagtgattgc gaaattgcct ccagaagcta
    16561 gacttgtctg tcagaaaaaa gccgaatctt tagctgttga cctttatcag gcaggtcaag
    16621 atttttcagt gctgaatggt gatttttcaa gctctttact aaatctttca cagctgaaca
    16681 taggcttaga aggagcctat cagcaggaga atgcggcctt ggcgttgcaa acttttcttc
    16741 tttttatggg agaaagaaag gaagctattg atgaacaggc tgtaagaaag gccttggaac
    16801 agacccattg ggctggtcgc ttggagcgta ttcgcccaca gatttacttg gatggtgctc
    16861 ataacctccc tgccttgact cgcttggttg agtttatcaa agaaaaagag caggaaggct
    16921 atcgacctca aatcctcttt ggatccttga aacgtaagga ttatcaaggg atgttgggtt
    16981 atctgactga aaatttacct caagtggaac tcaaaatgac cggctttgac tatcaagggt
    17041 ctttggacga gacagatgta acaggttacc atgtaattcc ctcttaccga gaatttatca
    17101 gcagtttcga agaaagggca gacgctcaag acttgctgtt cgttacaggg tctctctatt
    17161 ttatctcaga agtacggggc tacctgctgg accgtgagca gataaattga cccccttttt
    17221 tgaacttgtt atactagggg aattgctggt tagagaaaat ttctctaaat tggtagcaga
    17281 aaggaaattc atcatgaaat taaaaagatt cacactttct cttgcttctc tagcaagttt
    17341 tagtctctta gtagcttgtt cacaaagagc tcaacaggtt caacagcctg ttgctcagca
    17401 ggtccaacag cctgctcaac agaataccaa tactgcaaat gcaggaggta accaaaatca
    17461 agcggctcca gtacaaaacc aacctgttgc tcaaccgacc gatattgatg ggacttatac
    17521 tggtcaggat gacggagacc gtatcacttt agtggtaact ggaacgactg gtacatggac
    17581 tgagctcgaa tctgacgggg atcagaaagt caaacaggtt acatttgatt cagcaaatca
    17641 acgcatgatt attggcgatg atgtcaaaat ttacactgta aacggtaatc aaatcgtcgt
    17701 agatgatatg gatagagacc catcggacca aatcgtttta actaaataag actatgcaag
    17761 tagaaagggc tggatttttc agccttttta cttttaccta gaaaataatc ataaatactt
    17821 gccttctacc gaatacctgc gttatactag tatcaattac ttgtttttag cattcaaact
    17881 atcaaggagg ggatatgaaa tataggaaat ttcaattatt gatgtccaag tatggcttta
    17941 gtctttcgat tatgctactt gaactttgtc ttgtttttgg tctctttctt tatttaggac
    18001 gcatggctcc cattttatgg attactgtcc tcattctact gagtatcatc acaatcattt
    18061 cgatagtcaa ccgtaatacg actcctgaga ataaggtaac ctggttgtta gtagcctttg
    18121 tgccagtatt tggtcccttg ctctatctga tgtttggtga aaggcgattg tccaaaaaag
    18181 aaattaaaca actgaagaag ctaggctcta tgcatttcca agaagcaaat agccagctac
    18241 taaaagagaa attaaaagaa agtgacaagg cagcttatgg agtcatcaag tccttattga
    18301 gtatggatac caatgctgac atctatgatc aaactgcctc tacatttttt cctaacggag
    18361 aagctatgtg gaaaaagatg gtagaagatc ttaaaaaggc tgagaaattt attttcttgg
    18421 aatattacat tatagaagaa ggtttgatgt ggaatcgcat actagatata ctagagcaaa
    18481 aggtagctca gggtgtagag gttaagatgc tctatgatga tatcggctgt atggctactt
    18541 taacaggaga ttatgcacat cgacttcgtc agctgggcat cgaggcccat aaattcaata
    18601 aagttattcc tcgtttgaca gtggcttata ataacagaga tcatagaaaa atattgattg
    18661 ttgatggtca gatagcctat actggtgggg tcaatctggc agatgagtac attaaccacg
    18721 tcgagagatt tggttattgg aaggatagtg gaattcgctt agacggacta gcagtaaaag
    18781 ctctgacacg cttatttttg accacttggt acattaatcg aggagaaatt agtgattttg
    18841 atcaatatca tttagaaaat cattctatcc cgagtgacgg tttaaccatt ccatacggaa
    18901 gtggacccaa gccaattttt cgagcgcagg tagggaaaaa agtttatcag agtttaatca
    18961 atcaagcaac agaatcggtc tatattacga caccttattt gattatagat tatgatttaa
    19021 cagagacaat caaaaatgca gctatgagag gggtcgatgt tcgaattatc accccttaca
    19081 taccagataa gaagttcatt cagttagtca cgagaggagc ttatcccgac cttctttctg
    19141 ctggtgttcg gatttatgag tatagtccag gttttattca tagtaagcag atgttggtag
    19201 acgaagattt tgcggtggtg gggacaatca atctcgacta ccggagcttg gtacaccatt
    19261 atgaaaatgc agtcttactc tataaaactc cttctataag ggaaatcgcc cgagattttc
    19321 gaaatatatt tgcagattct caggaagtct atcctcattc tatcaaaacg agctggtatc
    19381 aaaagcttgt aaaagaaatc gcccagctat tcgcccctat cttataagaa atctagaact
    19441 taggatacaa accagaccta tttttcttgt cttttacgaa tagaaagaaa gaggagggga
    19501 attcatgcta actaaggaag aagtgaatga tatgattgag ttcaaacaga cacatttaca
    19561 ccgctttcgg gaaattgaaa cctttgtgaa actatgcaaa aaatcactca aacagccatc
    19621 gcaagctgac aatcctagga ctttttgata tactaagaca gataaattga acaggagaaa
    19681 cgatatgagt gtgtatggta gagtagaaga agttcataag gagaatcgtg aacctctaga
    19741 ataccaaatc gaacaagaat cgcatcatcg tgaatcaagt cgtcttcctt tggtgaaaat
    19801 tttactatgg agtacgcttg taacggggat aactctagga gtaccgctat tactcgactt
    19861 aatgagtgca caagaagtgc aggattttta tgcaggttgg gcgcttcatc agacagggaa
    19921 gatttacagc gactattatg gaagtcaagg tttgctttat tatttgctga cttacgtgag
    19981 tcagggcgga tttttctttg ccatctttga gtggttagcc ttggtagcag gaggattttt
    20041 cctttttaga tcagcggaca ccttgacaga gcaaggagac caagctggac atctggtgac
    20101 tattttttac atgctagtta caggtcttgc ttttggtgga ggctatgcga ctcttttagc
    20161 gcttcctttc ttattcgcag cctttagttt agttgcggct tacctaagca atccaagcca
    20221 tgataaggga tttgtacgga ttgggctagc tttggcaggc ggatttttct ttgctccctt
    20281 atcatcgctc ctgtttattg ctgtagtgag tttaggcttg ttggtcttta accttgggca
    20341 tagacgcttt gcgcatgggt tttatcagtt tcttgcagtg gctttaggtt tttcacttgt
    20401 cttttatcca actgcctact atagtgctgc aacaggaagt tttggggatg cgattagtgg
    20461 tattcgttat cctattgaca gtattcgctt tgattttact tctaaaattt tagagaatat
    20521 gtttttttat ggcttgttgt cccttggttt gggatttgtg gtttgtatct ttttaggtct
    20581 ctttcaatcc aaacccttta aactatacgt catttcggtt cctgcaagtc ttgtggtaat
    20641 tttaggtttg attttacttt tcttttcaca agagcctctg cacgcttctt atttgatggt
    20701 cgtcttccct gttttcctac ttttattggt aaccaatatt aagagtcaac agagggggcg
    20761 tagtgctaga agaagccgaa gagaaacgcc agttggccta tggagtcgtt tcttcaaagg
    20821 aaatctatat ctgctagttt ttgggtttgt ctatcttttg tctgttcctt ttttgatgaa
    20881 gtttgtcctt tatccagtac cttatcaaga acgtaatcgt cttgctgatt tggtaaaaga
    20941 ggagacaaat acggaagatg ctatctatgc atgggatgat actgcgactc tttatcgtaa
    21001 gagtgagcgc ttgtcgccat cggcgatttt gtccccgttg cactatacag caactgagga
    21061 aaatcgtaat aagttactta atgacttgaa agaaaaacaa cctaaggtga ttgtggtaaa
    21121 tgacaaggtg gaagtctggt ctgaagtgga aacactctta aaagaaaatt accaacaagt
    21181 aaagactgat tactcagagt ttaaagtcta taaaattaaa taaccaaatc aatatcttgt
    21241 gtatttttaa aaattttaag atttttaaca caagatattg atttttcttt ttagagtggt
    21301 ataatacttt ttagaaagaa cattttagaa aagagcatgc atatgattgc actagaagaa
    21361 aaaattacaa ttttgccaac tctcttcgtc gagaaacgag atgggagacg tgttgtattt
    21421 gatgtggaca agattgacaa ggctctccac aaggcggctg acaaggttat ggatgtgaca
    21481 cccctggttg aaaaacgcct caatgatctg actgagcgaa ttattacaga aattcatagt
    21541 cgctttccac agggaattaa gatttacgaa attcaaaata tcgtagaaca tgaactcctt
    21601 gaagccaaag aatatgcact ggctgaggag tatattactt atcggacaca gagggatttt
    21661 gagcgctcaa aagcgacgga tatcaacttt agtattcata aacttctcaa caaagaccag
    21721 acagttgtca atgaaaatgc taataaagac agtgatgtct ttaacactca gcgtgatttg
    21781 acagcaggga ttgttgggaa atcaatcgga ctgcaaatgc ttcctaagca cgtagccaat
    21841 gcccaccaaa agggggatat ccactatcac gatttggact acagtcccta tacccctatg
    21901 accaactgct gtttgattga ttttaagggt atgttggaaa atggttttaa gattggaaat
    21961 gcagaggtag agagtcccaa gtctatccag actgcgacag cacagatttc tcaaatcatt
    22021 gccaacgttg cttctagcca gtacggtggc tgttcagctg accgtatcga tgaaattttg
    22081 gcgccttatg cagagaagaa ttatcaaaaa catctcaaag atgcagaaga gtgggtattg
    22141 cctgaaaaac gggaagatta cgcttggaag aaagcgcaaa aggacatcta cgatgccatg
    22201 caatctcttg agtatgaaat caatactctc ttcacttcaa atggacaaac accttttact
    22261 tcgttaggtt ttggtctggg aaccagtcgt tttgaacgag aaattcaaaa agctatttta
    22321 aacattcgca tcaagggtct tggttcagaa caccgtacgg ctatctttcc taaacttatt
    22381 ttcactctga aaagaggcct taatttagag gaaggaactc ccaactacga catcaagcag
    22441 ttggctcttg agtgtgcaac caaacggatg tatccagatg ttttatctta cgataagatt
    22501 gttgacttga caggatcctt caaggtgcct atgggttgcc gttctttcct ccaaggatgg
    22561 aaggatgaaa atggtgtaga agtcaattca ggtcgcatga atctgggtgt tgtgacggtt
    22621 aatctgcctc gtattgctct tgagtctgaa ggtgatatga ataagttctg ggaaatcttc
    22681 aacgagcgaa tgaatatcgc agaagatgct cttgtttacc gtgtcgaacg cactaaagag
    22741 gcgacaccag caaatgctcc tattctttat cagtacggtg cttttggcca tcgtctaggt
    22801 aaagaagaaa gtgttgacca gctctttaag aatcgtcgtg ctaccgtttc gctgggctat
    22861 atcggcttgt atgaagtagc gacagttttc tttggtaaca gctgggaaag taatccagat
    22921 gctaaggaat tcacgctaga catcattcac gatatgaaac gccgtgtaga agagtggtca
    22981 gaccaatatg gctaccattt ctctatctac tcaacaccat ccgaaagtct gacagaccgt
    23041 ttctgccgac tagatataga caagtttggc tctattcctg atatcacaga caaggaatac
    23101 tacaccaact ctttccacta cgatgttcgt aaaaatccaa caccgtttga aaaattggac
    23161 tttgagaaag tctatccaga agcaggtgcg tcaggtggtt tcatccatta ttgtgagtat
    23221 ccagtccttc agcaaaatcc aaaggccttg gaagctgtct gggattatgc ttatgaccgt
    23281 gtaggctatc taggcaccaa tactccgatt gaccgttgct acaagtgtga ctttgaaggg
    23341 gattttgaac caactgagag agggtttgct tgtccaaact gtggcaatag cgaccctaaa
    23401 acagtagatg tggtgaagcg aacttgtggc tacctaggta atcctcaagc aagaccgatg
    23461 gtcaacgggc gtcacaagga aatcgctgcg cgtgtcaaac atatgaatgg ttcaacgatt
    23521 aaaatagctg ggcatcaagt aacaaattag aaagaaatga aatgggaaaa tatcaactag
    23581 acgataaggg gcgcgcacaa gtgacccgtt atcacgagaa acactctaaa ggtggagctg
    23641 gtaagaaaga acgcttgctt agcttcagag aacaattttt aaacaagaac aagaaaaaat
    23701 aaaagtgaga gccagctctc gcttttctca tagtgggagg taaggatgga attacgcaga
    23761 ccaagattag cggataagaa agctgtttta gatatgatga cagagtttga aaagagccaa
    23821 tcagcccatg atggaggatt ttgggatgtt gaggattttt cttacgagga gtggctagaa
    23881 ggtaaccgta ataaagaaat gggaatatgg ctacctgaaa atcgcgttcc atcgatacag
    23941 ttcgtattgt ttggtgaatc aggttgtgct ctaggatttt tgaatctgcg attgagactg
    24001 aatgaggggt tactgaatta tgctggccac attggctact ccatccgtcc atctgaaaga
    24061 ggcaagggtt atgcaaaaga gactctccgt cagggcttgc aagttgctaa ggaaaagaac
    24121 atcaagaaag ctctggtgac ctgtagcgtg aataatcctg ctagcagagc agtcattcta
    24181 gcaaatggtg gaatatttga ggatgctcgc aatggagtcg agcgttattg gatagaggta
    24241 gcgaatgaat aatccaaaac cacaagaatg gaaaagcgag gaacttagtc aaggtcgtat
    24301 cattgactac aaggccttta actttgttga tggcgaaggc gtgcgtaact ctctctatgt
    24361 agcaggctgt atgtttcact gcgaggggtg ttataatgta gcgacctggt ccttcaatgc
    24421 aggcattccc tatacagctg agttggaaga acagattatg gcagatcttg cccagcctta
    24481 tgttcagggg ttaaccttac taggagggga gccttttctt aatactggga ttctcttgcc
    24541 acttgttaag cggattcgga aggaattgcc agacaaggac atttggtcct ggaccggcta
    24601 cacttgggaa gaaatgatgt tggaaactcc agataaactg gaactcttgt cactgattga
    24661 cattcttgtc gatggaagat atgatcgaac taagagaaat cttatgctcc agtttcgagg
    24721 ttcgtctaac caacgaatta tcgatgtgca aaaatcgctc aaaagtgggc aagtagtgat
    24781 ttgggacaag ctcaatgacg gaaaagaaag ctatgaacag gtgaagagag aatgaagaaa
    24841 aaggacttag tagaccaact agtctcagag atcgagacgg ggaaagtcag gacactggga
    24901 atatacggtc atggagcttc aggtaaatca acctttgcac aggaattgta ccaagcttta
    24961 gattctacta cagtaaattt gctagagaca gatccttata tcacctcagg acgccatctg
    25021 gtagtaccca aggacgcgcc gaatcaaaag gtgacagcca gtctgccagt ggcgcatgaa
    25081 ctggagagtt tgcagagaga tatccttgcc ttgcaggcgg gtatggatgt cttgacaatt
    25141 gaagaacctt ggaaggctag tgaggtcttg tctggagtca aaccaatttt gattgtcgaa
    25201 gggatgtctg ttggctttct acccaaggaa ctctttgaaa aaaccatctg tttctacacg
    25261 gatgaggaga ccgaattaaa gcgacgcctt gctagagata cgactgtgag aaatcgtgat
    25321 gcatccttta tattggctag ccatcagatg agacgggagc agtatctgcg atactataaa
    25381 gaaactgagt ctaaggcgga tatcttagtg gatcaatcag aagataaatt tgatgtcaag
    25441 aggacttaac ttatgtatag aagaaatata gaagaaaatc cctaatttta ggaagaaaaa
    25501 gtaggaaaac agtttcatcc caaaaaaacg aaaaaaccta acaaatccct tgcaatcgca
    25561 ggggatttgt gttattctat tatggtgctg taaattacag ccttagtttt gatacaagag
    25621 gttgcgacac gctcggttgc attgccacgc aacaccgtgt cggttttctt gtggagctag
    25681 cctattatct tagatagacg aaaaggagaa aaagatggca aacaaaaaaa tccgtatccg
    25741 tttgaaagct tacgaacacc gtacgcttga cacagcggct gcaaaaatcg tagaatcagc
    25801 tactcgtaca ggtgcacaag ttgcgggtcc aatcccactt ccaactgaac gtagcctcta
    25861 cacaatcatt cgtgcgactc acaaatacaa agactctcgc gaacaatttg aaatgcgtac
    25921 acacaaacgt ttgatcgata tcgttaaccc aactcaaaaa acagttgatg ccttgatgaa
    25981 attggatctt ccaagtggtg taaacgtaga aatcaaactt taatctaaaa tataaaagag
    26041 cagaggctgg tgtttcaatc taattgaaca cggactaaac tcggtgtgaa aaagataaac
    26101 ttcctattgt ccgttagaca ctgcgttagt ttcctatttt cacgttgagt ttgacgccct
    26161 ttgtatctta gacttgagca taaaaaacgc tcgttaaaaa ctttttgaat aaaaaatata
    26221 gaaaaggaac tattttctca tgacaaaagg aatcttaggg aaaaaagtgg gaatgactca
    26281 aatcttcact gaagctggcg aattgatccc tgtaacagtt attgaagcaa ctccaaacgt
    26341 tgttcttcaa gttaaaactg ttgaaacaga cggatacaac gctatccaag ttggtttcga
    26401 tgacaaacgc gaagtattga gcaacaaacc tgctaaagga catgtagcga aagctaacac
    26461 ggctcctaag cgcttcattc gtgaattcaa aaacgttgaa ggcttggaag ttggcgctga
    26521 aatcacagtt gaaacattcg cagctggaga tgttgttgat gtaactggta cttctaaagg
    26581 taaaggtttc caaggtgtta tcaaacgcca cggacaatca cgtggaccaa tggctcacgg
    26641 ttctcgttac caccgtcgtc caggttctat ggggcctgtt gcacctaacc gcgtattcaa
    26701 aggtaaaaac cttgcaggac gtatgggtgg cgaccgcgta acaattcaaa accttgaagt
    26761 tgtacaagtt gttccagaaa agaacgttat ccttatcaaa ggtaacgtac caggtgctaa
    26821 gaaatctctt atcactatca aatcagcagt taaagctggt aaataataaa gaaaggggaa
    26881 atcagtcaca atggcaaacg taacattatt tgaccaaact ggtaaagaag ctggccaagt
    26941 tgttcttaac gatgcagtat ttggtatcga accaaatgaa tcagttgtgt ttgatgtaat
    27001 catcagccaa cgcgcaagcc ttcgtcaagg aacacacgct gttaaaaacc gctctgcagt
    27061 atcaggtggt ggacgcaaac catggcgtca aaaaggaact ggacgtgctc gtcaaggttc
    27121 tatccgctca ccacaatggc gtggtggtgg tgttgtcttc ggaccaactc cacgttcata
    27181 cggctacaaa cttccacaaa aagttcgtcg cctagctctt aaatcagttt actctgaaaa
    27241 agttgctgaa aacaaattcg tagctgtaga cgctctttca tttacagctc caaaaactgc
    27301 tgaatttgca aaagttcttg cagcattgag catcgattct aaagttcttg ttatccttga
    27361 agaaggaaat gaattcgcag ctctttcagc tcgtaacctt ccaaacgtga aagttgcaac
    27421 tgctacaact gcaagtgttc ttgacatcgc aaatagcgac aaacttcttg tcacacaagc
    27481 agctatctct aaaatcgagg aggttcttgc ataatgaatt tgtatgatgt tatcaaaaaa
    27541 cctgtcatca ctgaaagctc aatggctcaa cttgaagcag ggaaatatgt atttgaagtt
    27601 gacactcgtg cacacaaact tttgatcaag caagctgttg aagctgcttt cgaaggtgtt
    27661 aaagttgcca atgttaacac aatcaacgta aaaccaaaag ctaaacgtgt tggacgttac
    27721 actggtttta ctaacaaaac taaaaaagct atcatcacac ttacagctga ttctaaagca
    27781 atcgagttgt ttgctgctga agctgaataa tctaaggagg aaatatcgtg ggaattcgtg
    27841 tttataaacc aacaacaaac ggtcgccgta atatgacttc tttggatttc gctgaaatca
    27901 caacaagcac tcctgaaaaa tcattgcttg ttgcattgaa gagcaaggct ggtcgtaaca
    27961 acaacggtcg tatcacagtt cgtcaccaag gtggtggaca caaacgtttc taccgtttgg
    28021 ttgacttcaa acgtaataaa gacaacgttg aagcagttgt taaaacaatc gagtacgatc
    28081 caaaccgttc tgcaaacatc gctcttgtac actacactga cggtgtgaaa gcatacatca
    28141 tcgctccaaa aggtcttgaa gtaggtcaac gtatcgtttc aggtccagaa gcagatatca
    28201 aagtcggaaa cgctcttcca cttgctaaca tcccagttgg tactttgatt cacaacatcg
    28261 agttgaaacc aggtcgtggt ggtgaattgg tacgtgctgc tggtgcatct gctcaagtat
    28321 tgggttctga aggtaaatat gttcttgttc gtcttcaatc aggtgaagtt cgtatgattc
    28381 ttggaacttg ccgtgctaca gttggtgttg tcggaaacga acaacatgga cttgtaaacc
    28441 ttggtaaagc aggacgtagc cgttggaaag gtatccgccc aacagttcgt ggttctgtaa
    28501 tgaaccctaa cgatcaccca cacggtggtg gtgaaggtaa agcaccagtt ggtcgtaaag
    28561 caccatctac tccatggggc aaacctgctc ttggtcttaa aactcgtaac aagaaagcga
    28621 aatctgacaa acttatcgtt cgtcgtcgca acgagaaata atattaaact agtcgcttaa
    28681 gcaactagta aatccgccag ctcggtagcg ctccatagga gtgcaagccg ctgtggtaca
    28741 acatttaaag gagaaaatat aaaaatggga cgcagtctta aaaaaggacc tttcgtcgat
    28801 gagcatttga tgaaaaaagt tgaagctcaa gctaacgacg aaaagaaaaa agttattaaa
    28861 acttggtcac gtcgttcaac gatcttccca agtttcattg gttacactat tgcagtttat
    28921 gacggacgta aacacgtacc tgtttacatc caagaagaca tggtaggcca caaacttggt
    28981 gaatttgcac caacccgtac ttacaaaggt cacgctgcag acgacaagaa aacacgtaga
    29041 aaataaggag aacataaatg gcagaaatta cttcagctaa agcaatggct cgtacagtac
    29101 gtgtttcacc tcgtaaatca cgtcttgttc ttgataacat ccgtggtaaa agcgtagccg
    29161 atgcaatcgc aatcttgaca ttcactccaa acaaagctgc tgaaatcatc ttgaaagttt
    29221 tgaactcagc tgtagctaac gctgaaaaca actttggttt ggataaagct aacttggtag
    29281 tatctgaagc attcgcaaac gaaggaccaa ctatgaaacg tttccgtcca cgtgcgaaag
    29341 gttcagcttc accaatcaac aaacgtacag ctcacatcac tgtagctgtt gcagaaaaat
    29401 aaggaggtaa aatcgtgggt caaaaagtac atccaattgg tatgcgtgtc ggcatcatcc
    29461 gtgattggga tgccaaatgg tatgctgaaa aagaatacgc ggattacctt catgaagatc
    29521 ttgcaatccg taaattcgtt caaaaagaac ttgctgacgc agcagtttca actattgaaa
    29581 tcgaacgcgc agtaaacaaa gttaacgttt cacttcacac tgctaaacca ggtatggtta
    29641 tcggtaaagg tggtgctaac gttgatgcac tccgtgcaaa acttaacaaa ttgactggaa
    29701 aacaagtaca catcaacatc atcgaaatca aacaacctga tttggatgct caccttgtag
    29761 gtgaaggaat tgctcgtcaa ttggagcaac gtgttgcttt ccgtcgtgca caaaaacaag
    29821 caatccaacg tgcaatgcgt gctggagcta aaggaatcaa aactcaagta tcaggtcgtt
    29881 tgaacggtgc agatatcgcc cgtgctgaag gatactctga aggaactgtt ccgcttcaca
    29941 cacttcgtgc agatatcgat tacgcttggg aagaagcaga tactacatac ggtaaacttg
    30001 gtgttaaagt atggatctac cgtggtgaag ttcttccagc tcgtaaaaac actaaaggag
    30061 gtaaataacc aatgttagta cctaaacgtg ttaaacaccg tcgtgagttc cgtggaaaaa
    30121 tgcgcggtga agcaaaaggt ggaaaagaag tagcattcgg tgaatacggt cttcaagcta
    30181 caactagcca ctggatcact aaccgccaaa tcgaagctgc tcgtatcgcc atgactcgtt
    30241 acatgaaacg tggtggtaaa gtttggatta aaatcttccc acacaaatca tacactgcta
    30301 aagctatcgg tgtgcgtatg ggatctggta aaggggcacc tgaaggttgg gtagcaccag
    30361 ttaaacgtgg taaagtgatg ttcgaaatcg ctggtgtatc tgaagagatt gcacgtgaag
    30421 cgcttcgact tgctagccac aaattgccag ttaaatgtaa attcgtaaaa cgtgaagcag
    30481 aataaggaga aggcatgaaa cttaatgaag taaaagaatt tgttaaagaa cttcgtggtc
    30541 tttctcaaga agaactcgcg aagcgcgaaa acgaattgaa aaaagaattg tttgaacttc
    30601 gtttccaagc tgctactggt caattggaac aaacagctcg cttgaaagaa gttaaaaaac
    30661 aaatcgctcg tatcaaaaca gttcaatctg aagcgaaata atagactagg gaaggagaaa
    30721 tttcaatgga acgcaataat cgtaaagttc ttgttggacg tgttgtatct gacaaaatgg
    30781 acaagacaat cacagttgta gttgaaacaa aacgtaacca cccagtctat ggtaaacgta
    30841 ttaactactc taaaaaatac aaagctcatg atgaaaacaa tgttgccaaa gaaggcgata
    30901 tcgtacgtat catggaaact cgcccgcttt cagctacaaa acgtttccgt cttgtagaag
    30961 ttgttgaaga agcggtcatc atctaatcaa acctgaaagg agaaaactga aatgattcaa
    31021 acagaaactc gtttgaaagt cgcagacaac agcggtgctc gcgaaatctt gactatcaaa
    31081 gttcttggtg gttcaggacg taaatttgca aacatcggtg atgttatcgt ggcatctgta
    31141 aaacaagcta ctcctggtgg tgcggttaaa aaaggtgacg ttgttaaagc agttatcgtt
    31201 cgtactaaat caggtgctcg tcgtgctgat ggttcataca tcaaatttga cgaaaacgca
    31261 gcagttatca tccgtgaaga caaaactcct cgcggaacac gtatctttgg cccagttgca
    31321 cgtgaattgc gtgaaggtgg cttcatgaag atcgtgtcac ttgctccaga agtactttaa
    31381 tttttaggaa caaactagtc ccctagcttc aagctagggt gcccttatgg gcgtaagaaa
    31441 aatcaaggag aaacctaatg tttgtaaaaa aaggcgacaa agttcgcgta atcgctggta
    31501 aagataaggg aacagaagct gttgtcctta ctgcccttcc aaaagtaaac aaagttatcg
    31561 ttgaaggtgt taacattgtt aagaaacacc aacgtccaac taacgagctt cctcaaggtg
    31621 gtatcatcga gaaagaagca gctatccacg tatcaaatgt tcaagttttg gacaaaaatg
    31681 gtgtagctgg tcgtgttggt tacaaatttg tagacggtaa aaaagttcgc tacaacaaaa
    31741 aatcaggcga agtgcttgat taatcacgaa ggaaaggaga agtataatgg caaatcgttt
    31801 aaaagaaaaa tatcttaatg aagtagttcc tgctttgaca gaacaattca actactcatc
    31861 agtgatggct gtgcctaaag tagataagat tgttttgaac atgggtgttg gtgaagctgt
    31921 atcaaacgct aaaagccttg aaaaagctgc tgaagaattg gcacttatct caggtcaaaa
    31981 accacttatc actaaagcta aaaaatcaat cgccggcttc cgtcttcgtg aaggtgttgc
    32041 gatcggtgca aaagttaccc ttcgtggtga acgtatgtac gaattcttgg ataaattggt
    32101 atcagtttca cttccgcgtg tacgtgactt ccacggtgtt ccaacaaaat catttgatgg
    32161 acgcgggaac tacacacttg gtgtgaaaga acaattaatc ttcccagaaa tcaacttcga
    32221 tgacgttgac aaaactcgtg gtcttgacat cgttatcgta acaactgcta acactgacga
    32281 agagtcacgt gcattgctta caggccttgg aatgcctttt gcaaaataat ataggaggta
    32341 aatctaatgg ctaaaaaatc aatggtagct agagaggcta aacgccaaaa aattgttgac
    32401 cgttatgctg aaaaacgtgc tgcattaaag gcggcagggg actacgaagg tttatctaaa
    32461 ttacctcgca acgcctcacc gactcgttta cataatcgtt gtagagttac ggggcgccca
    32521 cattcagttt accgcaaatt tggtctgagt cgtatcgctt ttcgcgaact tgcgcataaa
    32581 ggtcaaattc ctggtgtaac aaaagcatct tggtaattta agatatcaag agcgtcaaaa
    32641 ctccaagtaa aaataggaaa cttgacgaag aaactaaagt ttctaggaaa gtttatcttt
    32701 ttcacacaga gtttagcccg ggttcaattg ggcttgccaa tttgaacacg agctacagct
    32761 ttggcaaaaa agaccaattt gctttggagc attgcttctg cattaaattg tctatttttg
    32821 ctcgtgctgt tacgctcttt gtatcatgta ttaactagca agtgcaactt gcaaactact
    32881 agtaagagga gaaaaacaaa atggttatga ctgacccaat cgcagacttc ctaactcgta
    32941 ttcgtaatgc taaccaagct aaacacgaag tacttgaagt acctgcatca aacatcaaaa
    33001 aagggattgc tgaaatcctt aaacgcgaag gttttgtaaa aaacgttgaa atcattgaag
    33061 atgacaaaca aggcgtcatc cgtgtatttc ttaaatacgg accaaatggt gagaaagtta
    33121 tcactaactt gaaacgtgtt tctaaaccag gacttcgtgt ctacaaaaaa cgtgaagacc
    33181 ttccaaaagt tcttaacgga cttggaattg ccatcctttc aacttctgaa ggtttgctta
    33241 ctgataaaga agcacgccaa aagaatgttg gtggtgaggt tatcgcttac gtttggtaaa
    33301 atcaagatac aaagctcgta aagaacaaag caaaattagg aagttggaga agtttgttta
    33361 caaacaagcc aacttatcta ttttgcacag ttcttagagc gtgttcagtt cagctcttga
    33421 actaaataag tatctgaacc ccgtgaaaac tggccgttct ggcctgacaa tttaacagga
    33481 gaaaataaac atgtcacgta ttggtaataa agttatcgtg ttgcctgctg gtgttgaact
    33541 cgctaacaat gacaacgttg taactgtaaa aggacctaaa ggagaactta ctcgtgagtt
    33601 ctcaaaagat attgaaatcc gtgtggaagg tactgaaata actcttcacc gtccaaacga
    33661 ttcaaaagaa atgaaaacta tccacggaac tactcgtgcc cttttgaaca acatggttgt
    33721 tggtgtatca gaaggattca agaaagaact tgaaatgcgt ggggttggtt accgtgcaca
    33781 gcttcaagga tctaaacttg ttttggctgt tggtaaatct catccagacg aagttgaagc
    33841 tccagaagga attacttttg aacttccaaa cccaacaaca atcgttgtta gcggaatttc
    33901 aaaagaagta gttggtcaaa cagctgctta cgtacgtagc cttcgttcac cagaaccata
    33961 taaaggtaaa ggtatccgtt acgttggtga attcgttcgc cgtaaagaag gtaaaacagg
    34021 taaataatgt tgagtggttg atcatcaacc accaacctat tttccaactt tgtgcatagc
    34081 acacgattta aaactaaaga ggtgaaaact gtgatttcaa aaccagataa aaacaaactc
    34141 cgccaaaaac gccaccgtcg cgttcgcgga aaactctctg gaactgctga tcgcccacgt
    34201 ttgaacgtat tccgttctaa tacaggcatc tacgctcaag tgattgatga cgtagcgggt
    34261 gtaacgctcg caagtgcttc aactcttgat aaagaagttt caaaaggaac taaaactgaa
    34321 caagccgttg ctgtcggtaa actcgttgca gaacgtgcaa acgctaaagg tatttcagaa
    34381 gtggtgttcg accgcggtgg atatctatat cacggacgtg tgaaagcttt ggctgatgca
    34441 gctcgtgaaa acggattgaa attctaatag gaggacacta gaaaatggca tttaaagaca
    34501 atgcagttga attagaagaa cgcgtagttg ctgtcaaccg tgttacaaaa gttgttaaag
    34561 gtggacgtcg tcttcgtttc gcagctcttg ttgttgttgg tgaccacaat ggtcgcgtag
    34621 gatttggtac tggtaaagct caagaagttc cagaagcaat ccgtaaagca gtagatgatg
    34681 ctaagaaaaa cttgatcgaa gttcctatgg ttggaacaac aatcccacac gaagttcttt
    34741 cagaattcgg tggagctaaa gtattgttga aacctgctgt agaaggttct ggagttgccg
    34801 ctggtggtgc agttcgtgcc gttgtggaat tggcaggtgt ggcagatatt acatctaaat
    34861 cacttggttc taacactcca atcaacattg ttcgtgcaac tgttgaaggt ttgaaacaat
    34921 tgaaacgcgc tgaagaaatt gctgcccttc gtggtatttc agtttctgat ttggcataag
    34981 aaaggggata aaatggctca aattaaaatt actttgacta agtctccaat cggacgcatt
    35041 ccatcacaac gtaaaactgt tgtagcactt ggacttggca aattgaacag ctctgttatt
    35101 aaagaagata acgctgctat ccgtggtatg atcacagcag tatctcactt agtaacagtt
    35161 gaagaagtaa actaatgaag ttttagggga tgtgcactgt accatcccct aaaactagat
    35221 atagtcatct atgatgacat cgtataggcg agttgatggg ggagacaacc ttttctccct
    35281 tatcggcgct agcattttac aaaagaggag aaaataaaaa tgaaacttca tgaattgaaa
    35341 cctgcagaag gttctcgtaa agtacgtaac cgcgttggtc gtggtacttc atcaggtaac
    35401 ggtaaaacat ctggtcgtgg tcaaaaaggt caaaaagctc gtagcggtgg cggagttcgc
    35461 cttggttttg aaggtggaca aactccattg ttccgtcgtc ttccaaaacg tggattcact
    35521 aacatcaacg ctaaagaata cgcaattgtg aaccttgacc aattgaacgt ctttgaagat
    35581 ggtgctgaag taactccagt tgttcttatc gaagcaggaa ttgttaaagc tgaaaagtca
    35641 ggtattaaaa ttcttggtaa cggtgagttg actaagaaat tgactgtgaa agcagctaaa
    35701 ttctctaaat cagctgaaga agctatcact gctaaaggtg gttcagtaga agtcatctaa
    35761 gagaggtgac ctatgttttt taaattatta agagaagctc ttaaagtcaa gcaggttcga
    35821 tcaaaaattt tatttacaat ttttatcgtt ttggtctttc gtatcggaac tagcattaca
    35881 gttcctggtg tgaatgccaa tagcttgaat gctttaagtg gattatcctt cttaaacatg
    35941 ttgagcttgg tgtcggggaa tgccctaaaa aacttttcga tttttgccct aggagttagt
    36001 ccctatatca ccgcttctat tgttgtccaa ctcttgcaaa tggatatttt acccaagttt
    36061 gtagagtggg gtaaacaagg ggaagtaggt cgaagaaaat tgaatcaagc tactcgttat
    36121 attgctctag ttctcgcttt tgtgcaatct atcgggatta cagctggttt taataccttg
    36181 gctggagctc aattgattaa aactgcttta actccacaag tttttctgac gattggtatc
    36241 atcttaacag ctggtagtat gattgtcact tggttgggtg agcaaattac agataaggga
    36301 tacggaaacg gtgtttccat gattatcttt gccgggattg tttcctcaat tccagagatg
    36361 attcagggca tctatgtgga ctactttgtg aacgtcccaa gtagccgtat cacttcatct
    36421 atcattttcg taatcatttt gattattact gtattgttga ttatttactt tacaacttat
    36481 gttcaacaag cagaatacaa aattccaatc caatatacta aggttgcaca aggtgctcca
    36541 tctagctctt accttccgtt aaaggtaaat cctgctggag ttatccctgt tatctttgcc
    36601 agttcgatta ctgcagcgcc tgcggctatt cttcagtttt tgagtgccac aggtcatgat
    36661 tgggcttggg taagggtagc acaagagatg ttggcaacta cttctccaac tggtattgcc
    36721 atgtatgctt tgttgattat tctctttaca ttcttctata cgtttgtaca gattaatcct
    36781 gaaaaagcag cagagagcct acaaaagagt ggtgcctata tccatggagt tcgtcctggt
    36841 aaaggtacag aagaatatat gtctaaactt cttcgtcgtc ttgcaactgt tggttccctc
    36901 ttccttggtg tgatttccat tttaccgatt gcagctaaag atgtatttgg tctttctgat
    36961 gttgttgcct ttggtggaac aagtctcttg atcattatct ctacaggtat cgaaggaatc
    37021 aagcaattgg aaggttacct attgaaacgt aagtatgttg gtttcatgga cagaacagaa
    37081 taaaagtatt tactgaatca gtaaatactg agggagtgga ggtttaaact ctgacatttg
    37141 taagagttgg atctcccctc ttctattttg tttttaaata ggggtgaaaa gactttttgc
    37201 ttctatttaa aaataaaata aggagatcaa atcatgaatc ttttgattat gggcttacct
    37261 ggtgcaggta agggaactca agcagcaaaa atcgtagaac aattccatgt tgcacatatc
    37321 tcaacaggtg atatgttccg cgctgcaatg gcaaatcaaa ctgaaatggg tgttcttgct
    37381 aagtcatata ttgacaaggg tgaattggtt cctgacgaag ttacaaatgg aatcgtaaaa
    37441 gaacgtcttt cacaagatga tattaaagaa acaggattct tattggatgg ttacccacgt
    37501 acaattgaac aagctcaggc cttggacaaa acattggctg aacttggcat tgaactagaa
    37561 ggtgttatca atattgaagt gaaccctaac agccttttgg aacgtttgag tggccgtatc
    37621 atccaccgcg taactggaga aactttccac aaggtcttta acccaccagt tgactataaa
    37681 gaagaagatt actaccaacg tgaagatgat aagcctgaga cagtaaaacg tcgtttggat
    37741 gttaatattg ctcaaggaga accaatcatt gctcactacc gtgccaaagg tttggttcat
    37801 gacatcgaag gtaatcaaga tatcaatgat gtcttctcag atattgaaaa agtattgaca
    37861 aatttgaaat aaagcgtttt tcacacttgc caaaatccgc tacaaatgtt atactgagat
    37921 agtctgactt ataattgttg tctctgtgtc tagaggcatc gaatcgaaat ttatggaggt
    37981 gcttttgcgt ggcaaaagac gatgtgattg aagttgaagg caaagtagtt gatacaatgc
    38041 cgaatgcaat gtttacggtt gaacttgaaa atggacatca gattttagca acagtttctg
    38101 gtaaaattcg taaaaactat attcgtattt tagcgggaga tcgtgttact gtcgaaatga
    38161 gtccatatga cttgacacgt ggacgtatca cttaccgctt taaataatcg aaaaacttgg
    38221 agggataaga aatgaaagta agaccatcgg tcaaaccaat ttgcgaatac tgtaaagtta
    38281 ttcgtcgtaa tggtcgtgtt atggtaattt gcccagcaaa tccaaaacac aaacaacgtc
    38341 aaggataaga tagaaaggag aaaacatggc tcgtattgct ggagttgata ttccaaatga
    38401 caaacgcgta gtaatctcat tgacttatgt ttatggtatc ggacttgcaa catctaagaa
    38461 aattttggct gctgctggaa tctcagaaga tgttcgtgta cgtgatctta catcagatca
    38521 agaagatgct atccgtcgtg aagtggatgc aatcaaagtt gaaggtgacc ttcgtcgtga
    38581 agtaaacttg aacatcaaac gtttgatgga aatcggttca taccgtggta tccgtcaccg
    38641 tcgtggactt cctgtccgtg gacaaaacac taaaaacaac gctcgcactc gtaaaggtaa
    38701 agctgttgcg attgctggta agaaaaaata atataggagg taaaagtctt ggctaaacca
    38761 acacgtaaac gtcgtgtgaa aaagaatatc gaatctggta ttgctcatat tcacgctaca
    38821 tttaataaca ctattgttat gattactgat gtgcatggta atgcaattgc ttggtcatca
    38881 gctggtgctc ttggtttcaa aggttctcgt aaatctacac cattcgctgc tcaaatggct
    38941 tctgaagctg ctgctaaatc tgcacaagaa cacggtctta aatcagttga agttactgta
    39001 aaaggtccag gttctggtcg tgagtcagct attcgtgcgc ttgctgccgc tggtcttgaa
    39061 gtaacagcaa ttcgtgatgt gactccagtg ccacacaatg gtgctcgtcc tccaaaacgt
    39121 cgccgtgtat aatcatcgca ttacactgct tttcgtttaa gagggagtaa ctaaatgatc
    39181 gagtttgaaa aaccaaatat aacaaaaatt gatgaaaata aagattatgg caagtttgta
    39241 atcgaaccac ttgaacgtgg ctacggtaca actcttggta actctcttcg tcgtgtactt
    39301 ctagcttctc taccaggagc agctgtgaca tctatcaaca ttgatggtgt gttacatgag
    39361 tttgacacag ttccaggtgt tcgtgaagac gtgatgcaaa tcattctgaa cattaaagga
    39421 attgcagtga aatcgtacgt tgaagacgaa aaaatcatcg aactggatgt tgaaggtcct
    39481 gctgaagtaa cagctggtga cattttgaca gatagcgata ttgaaattgt aaatccagat
    39541 cattatctct ttacaatcgg tgaaggttct tctctaaaag cgactatgac tgttaacagt
    39601 ggtcgtggat atgtacctgc tgatgaaaat aaaaaggata atgcaccagt tggaacactt
    39661 gctgtagatt ctatttatac accagttaca aaagtcaact atcaagtgga acctgctcgt
    39721 gtaggtagca atgatggttt cgacaaatta acccttgaaa tcttgacaaa tggaacaatt
    39781 attccagaag atgctttagg gctttcagca cgtattttga cagaacatct tgatttgttt
    39841 acaaatctta ctgagattgc taagtcaact gaagtgatga aagaagctga tactgaatct
    39901 gacgaccgta ttttagatcg tacgattgag gaactggact tgtctgtgcg ttcatacaac
    39961 tgtttaaaac gtgccggtat caatactgtg catgatttga cagaaaaatc tgaagcagag
    40021 atgatgaaag tacgaaatct tggacgcaag agtttggaag aagtgaaact caaactcatt
    40081 gatttgggtc ttggattaaa agataaataa aggaggaata catggcttac cgtaaactag
    40141 gacgcactag ctcacaacgt aaagcaatgc ttcgcgattt gacaactgac cttttgatca
    40201 acgaatcaat cgtgacaact gaagctcgtg ctaaagaaat ccgtaaaact gttgaaaaaa
    40261 tgattactct aggtaaacgt ggtgatttgc atgcacgtcg tcaagcagct gctttcgtac
    40321 gtaatgaaat cgcatctgaa aactatgatg aagcaactga taagtacact tctactacag
    40381 cacttcaaaa attgttctca gaaatcgcac ctcgttatgc tgaacgtaac ggtggataca
    40441 ctcgtatcct taaaactgaa ccacgtcgtg gtgatgcagc gccaatggcg atcatcgaat
    40501 tagtataaaa tcatcaattt tgttgagtgt tatgatgatg gagtcttgtg ctcttagtct
    40561 agctctggtc taccgctagg atttcggtcc tagcgggaac actcatcata agttgggata
    40621 gtagacgctt gtttacgaaa ttgttttttt cttaagaaca acttcgtaag caggcgtttt
    40681 tgagtatttt cgttagaatt atgctatact atttgaaaag aatcctgttt aatgttaagg
    40741 tttcttattt taagaagaat tggagtttgc ttatgaaagc gattataact gttgttggta
    40801 aagataaatc tggaattgtt gcaggtgttt ctggtaaaat tgcagaatta ggattgaata
    40861 ttgacgatat ctctcaaact gtcttggatg aatattttac gatgatggct attgtatcta
    40921 gtgatgaaaa gcaagatttt acctatcttc gtaatgaatt tgaagctttt gggcaaactt
    40981 tgaatgtaaa aatcaatatt cagagtgcag cgattttcga agctatgtat aatatctagg
    41041 aggttatcat ggatattaga caagttactg aaaccatcgc catgattgag gagcaaaatt
    41101 tcgatatcag aaccattacc atggggattt ctcttttgga ctgtatcgat ccagatatca
    41161 atcgtgctgc ggagaaaatc tatcaaaaaa ttacgacaaa ggcggctaat ttagtagctg
    41221 ttggtgatga aattgcggct gagttgggaa ttcctatcgt taataagcgt gtatcggtga
    41281 cacctatttc tctgattggg gcagcgacag atgcgacgga ctacgtggtt ctggcaaaag
    41341 cgcttgataa ggctgcgaaa gagattggtg tggactttat tggtggtttt tctgccttag
    41401 tacaaaaagg ttatcaaaag ggagatgaga ttctcatcaa ttccattcct cgcgctttgg
    41461 ctgagacgga taaggtctgc tcgtcagtca atatcggctc aaccaagtct ggtattaata
    41521 tgacggctgt ggcagatatg ggacgaatta tcaaggaaac agcaaatctt tcagatatgg
    41581 gagtggccaa gttggttgta ttcgctaatg ctgttgagga caatccattt atggcgggtg
    41641 cctttcatgg tgttggggaa gcagatgtta tcatcaatgt cggagtttct ggtcctggtg
    41701 ttgtgaaacg tgctttggaa aaagttcgtg gacagagctt tgatgtagta gccgaaacag
    41761 ttaagaaaac tgcctttaaa atcactcgta tcggtcaatt ggttggtcaa atggccagtg
    41821 agagactggg tgtggagttt ggtattgtgg acttgagttt ggcaccaacc cctgcggttg
    41881 gagactctgt ggcacgtgtc cttgaggaaa tggggctaga aacagttggc acgcatggaa
    41941 cgacggctgc cttggccctc ttgaacgacc aagttaaaaa gggtggagtg atggcctgta
    42001 accaggtcgg tggtttatct ggtgccttta tccctgtttc tgaggatgaa ggaatgattg
    42061 ctgcagtgca aaatggctct cttaatttag aaaaactaga agctatgacg gctatctgtt
    42121 ctgttggatt ggatatgatt gccatcccag aagatacgcc tgctgaaact attgcggcta
    42181 tgattgcgga tgaagcagca atcggtgtta tcaacatgaa aacaacagct gttcgtatca
    42241 ttcccaaagg aaaagaaggc gatatgattg agtttggtgg tctattagga actgcacccg
    42301 ttatgagggt taatggggct tcgtctgtcg acttcatctc tcgcggtgga caaatcccag
    42361 caccaattca tagttttaaa aattaagaaa ataggagaaa ttttaagttc tatttaagat
    42421 ta
//
LOCUS       DAVKHZ010000010        36849 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000010 DAVKHZ010000000
VERSION     DAVKHZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.010"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..441)
                     /locus_tag="VWW99_000492"
     CDS             complement(<1..441)
                     /locus_tag="VWW99_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538380.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAF"
     gene            complement(629..1279)
                     /locus_tag="VWW99_000493"
     CDS             complement(629..1279)
                     /locus_tag="VWW99_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9538381.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            1391..2233
                     /locus_tag="VWW99_000494"
     CDS             1391..2233
                     /locus_tag="VWW99_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9538382.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            2309..2746
                     /locus_tag="VWW99_000495"
     CDS             2309..2746
                     /locus_tag="VWW99_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9538383.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            2814..3116
                     /locus_tag="VWW99_000496"
     CDS             2814..3116
                     /locus_tag="VWW99_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538384.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(3207..4280)
                     /locus_tag="VWW99_000497"
     CDS             complement(3207..4280)
                     /locus_tag="VWW99_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9538385.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            4436..5113
                     /locus_tag="VWW99_000498"
     CDS             4436..5113
                     /locus_tag="VWW99_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538386.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            5106..6080
                     /locus_tag="VWW99_000499"
     CDS             5106..6080
                     /locus_tag="VWW99_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9538387.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6129..8072)
                     /gene="thrS"
                     /locus_tag="VWW99_000500"
     CDS             complement(6129..8072)
                     /gene="thrS"
                     /locus_tag="VWW99_000500"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9538388.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEARKIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(8130..8306)
                     /locus_tag="VWW99_000501"
                     /pseudo
     CDS             complement(8130..8306)
                     /locus_tag="VWW99_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            complement(8884..9084)
                     /locus_tag="VWW99_000502"
     CDS             complement(8884..9084)
                     /locus_tag="VWW99_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538389.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            9323..9952
                     /locus_tag="VWW99_000503"
     CDS             9323..9952
                     /locus_tag="VWW99_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9538390.1"
                     /translation="MKLAVIAANGQVGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            10093..10362
                     /gene="rpsO"
                     /locus_tag="VWW99_000504"
     CDS             10093..10362
                     /gene="rpsO"
                     /locus_tag="VWW99_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9538391.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            11096..11710
                     /locus_tag="VWW99_000505"
     CDS             11096..11710
                     /locus_tag="VWW99_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9538392.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(12003..12752)
                     /locus_tag="VWW99_000506"
     CDS             complement(12003..12752)
                     /locus_tag="VWW99_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9538393.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            12885..15221
                     /locus_tag="VWW99_000507"
     CDS             12885..15221
                     /locus_tag="VWW99_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9538394.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            15323..16000
                     /locus_tag="VWW99_000508"
     CDS             15323..16000
                     /locus_tag="VWW99_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9538395.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAERILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            15987..16784
                     /locus_tag="VWW99_000509"
     CDS             15987..16784
                     /locus_tag="VWW99_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9538396.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            16801..17904
                     /locus_tag="VWW99_000510"
     CDS             16801..17904
                     /locus_tag="VWW99_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9538397.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSLGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            complement(17953..18054)
                     /locus_tag="VWW99_000511"
                     /pseudo
     CDS             complement(17953..18054)
                     /locus_tag="VWW99_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
     gene            18159..19178
                     /gene="galE"
                     /locus_tag="VWW99_000512"
     CDS             18159..19178
                     /gene="galE"
                     /locus_tag="VWW99_000512"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9538398.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKVMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            19180..20145
                     /locus_tag="VWW99_000513"
     CDS             19180..20145
                     /locus_tag="VWW99_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9538399.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRISREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(20196..20402)
                     /locus_tag="VWW99_000514"
     CDS             complement(20196..20402)
                     /locus_tag="VWW99_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9538400.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            20454..20930
                     /locus_tag="VWW99_000515"
     CDS             20454..20930
                     /locus_tag="VWW99_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9538401.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            20939..21610
                     /locus_tag="VWW99_000516"
     CDS             20939..21610
                     /locus_tag="VWW99_000516"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9538402.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            21741..22079
                     /locus_tag="VWW99_000517"
     CDS             21741..22079
                     /locus_tag="VWW99_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9538403.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            22082..22711
                     /locus_tag="VWW99_000518"
     CDS             22082..22711
                     /locus_tag="VWW99_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9538404.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            22951..24102
                     /locus_tag="VWW99_000519"
     CDS             22951..24102
                     /locus_tag="VWW99_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9538405.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            24415..25164
                     /gene="truA"
                     /locus_tag="VWW99_000520"
     CDS             24415..25164
                     /gene="truA"
                     /locus_tag="VWW99_000520"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9538406.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            25154..25927
                     /locus_tag="VWW99_000521"
     CDS             25154..25927
                     /locus_tag="VWW99_000521"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9538407.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            25905..26366
                     /locus_tag="VWW99_000522"
     CDS             25905..26366
                     /locus_tag="VWW99_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9538408.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(26625..26828)
                     /locus_tag="VWW99_000523"
     CDS             complement(26625..26828)
                     /locus_tag="VWW99_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9538409.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(26821..27903)
                     /locus_tag="VWW99_000524"
     CDS             complement(26821..27903)
                     /locus_tag="VWW99_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9538410.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(28029..28811)
                     /locus_tag="VWW99_000525"
     CDS             complement(28029..28811)
                     /locus_tag="VWW99_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9538411.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(28811..30154)
                     /locus_tag="VWW99_000526"
     CDS             complement(28811..30154)
                     /locus_tag="VWW99_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9538412.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(30253..31569)
                     /locus_tag="VWW99_000527"
     CDS             complement(30253..31569)
                     /locus_tag="VWW99_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9538413.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            31762..32988
                     /locus_tag="VWW99_000528"
     CDS             31762..32988
                     /locus_tag="VWW99_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9538414.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            33187..34776
                     /locus_tag="VWW99_000529"
     CDS             33187..34776
                     /locus_tag="VWW99_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9538415.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNK
                     KPRNTLSEKQTGFVIRNKGDK"
     gene            35040..35828
                     /gene="codY"
                     /locus_tag="VWW99_000530"
     CDS             35040..35828
                     /gene="codY"
                     /locus_tag="VWW99_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9538416.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            35828..36403
                     /locus_tag="VWW99_000531"
     CDS             35828..36403
                     /locus_tag="VWW99_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9538417.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELFE"
     gene            36485..36570
                     /locus_tag="VWW99_000532"
     tRNA            36485..36570
                     /locus_tag="VWW99_000532"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36519..36521,aa:Leu,seq:aag)
     gene            complement(36693..>36849)
                     /locus_tag="VWW99_000533"
     CDS             complement(36693..>36849)
                     /locus_tag="VWW99_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538418.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSQA"
BASE COUNT        10452 a         6932 c         8387 g        11078 t
ORIGIN      
        1 aaaggcaata gttgttacgt aggcaaggag attcatcttg cccccatatc cgatatagtt
       61 ggtcacaaag gcaaagagga aggcgatgat ggaaatgatg atggccgcca attttacctg
      121 tttttggctc atttggttgg gtctgccttc ttgcgaagct ttccatttct ttatagcaaa
      181 ggtataaatg aggaaggtga cgggataggt aatgatggcc gccttatttc caaggatata
      241 atcaatagca ccggacaaaa tggtattaac aataccaaag taatttcccc atttgcttaa
      301 tttccccgtg aaacgagtgg acaacatgga aatcccaacg ttggttacgg aaatcaatcc
      361 aaagggtaca agagctgtcc atggtcccca gtctacaaat ttatcgaggc gtgagttgag
      421 gtaaccagat gcaatcgcaa tccaacgacc aaagcaaccc cgaagaggtc aaactattta
      481 gatgtagcaa aaatttttag tgattttttc ataggttaaa ctacctttct ttttttcaaa
      541 tactctctca ccaaatgaaa gtaaaataaa atgatagaaa taaaaccctg aaaataaaac
      601 tttcagggtt ggtaggggac ttgagaaatt agttgatttt ctcgacatag agttgttttg
      661 caatgtccat attcacttgt aaatcctcgt cgttactgag gatagtgaag gtattgctga
      721 agccatcaaa ctgcttgact tggagctggt caccgatgtg aagtgagtgc ttgtccagat
      781 aatggagaat gtcaaaatta tcgtgcaccc gagtcaggcg gtaggcgcca gcttccttga
      841 tatcagctag tgggaggtta ttgatttcaa cgaggagttc tcccttggca ggaatagttc
      901 ctccgtgggg gcaggtttta gggaaaccta gcagtttatc tagtctttcc acgaacaggt
      961 cagagacagt gtgttccaag acctcagctt cctcgtgaat ctggtcactt gtatagtcta
     1021 aatgatgaac tagaaaaact tcaatcaagc ggtgcttacg atagagctca gagaccagtt
     1081 tgagaccgag gtcagtcagt agatagccac attccttgtc ctttaggatg agattttcac
     1141 ttttcattcg tttgatcatt tcagttacgg cagggggaga gacttgcatg cgagccgcaa
     1201 tttccttgtt ggtaatctta tgcaggtcta tgccaatttc ataaatacat tttagatagt
     1261 cttctttgtt tggggtcatt cgtttcctct tgtctaatat ctttatctag tatatcaaaa
     1321 aaagctagat ttctctagct gtgacttttt atgttatact tattgcaaga gaaaaaacga
     1381 ggagatggat atgacgaaaa tagctcttct ttcagatatt catggaaata ccaccgcctt
     1441 ggaggctgtt ttggcagatg ctcggcagct aggagtggat gaatactggc ttttgggaga
     1501 tattctcatg ccagggacag ggcgtagaag gattttggac ttgttggatc aactaccgat
     1561 tacggctaga gttttgggaa actgggaaga cagtctttgg catggtgtcc gtaaggaatt
     1621 ggacagtact cgccccagtc aacgctatct cttgcgccag tgccagtatg ttttagagga
     1681 aatttcccta gaagaaattg aagtgctcca caatcaacct cttcaaattc atcgtcagtt
     1741 tggggatttg acggtgggaa ttagccatca tctgcctgat aagaactggg ggcgagagtt
     1801 gattcatact ggcaaacaag aggagtttga ccgcttggtg actcatcctc cctgtgatat
     1861 tgctgtttat ggacatattc accagcagtt gcttcgttac gggactggtg ggcaattgat
     1921 tgtcaatccg ggttcgattg gccaaccttt ctttttggac gctcagctac ggaaggactt
     1981 gcgggcccag tatatgattt tggagtttga tgacaaaggc ctggtagata tggacttccg
     2041 acgggtagac tacgatgtgg cagcggaatt gcagctggct aaagacctga gacttcccta
     2101 ttttgaggtt tactatgaaa gtctggtcaa tgggatccac catactcatc atcaggaatt
     2161 tttgagagaa ttggctcaga aagagggctg cgatcgggag ttagacgact ggttgaaaag
     2221 tggtaacgat tgacattaca actaaaaagg gtataataat agaaaaagaa gagtagaggc
     2281 agggtagcct cgtaaaaagg agaagaggat gcaaattcca agtagattta ccattgcgac
     2341 tcatatgctg ataatcattg ccctcgaggg gaaggaaagc aaggtgacca gtgattttct
     2401 ggctgctagt gtcggggtca atcctgtcat tatcagaaag atcttgtccc agttgaagaa
     2461 ggcagagctg atttcagtag cgcgtggaac gggcggaaca gagattgtca aggaccttaa
     2521 ggatattagt cttttagatg tttatcaggc ggtcgaatgt cttggtaaga caggtcaact
     2581 cttcagtttc catgacaatc cgaatccaaa ttgccctgta ggagctcata ttcatgatgt
     2641 tttggatcaa aaattggaga gaattcagtt gactatggag gcagaacttg gtcaaaccag
     2701 tctagaaaaa gtcgtggccg atgcagagag tcagatgaag gattaagagg gataaatgcc
     2761 ctcttttttc tttggataat tatgataaaa tgtagctatt aaaggagtaa gaaatgtatc
     2821 tcgttataga aattgtatta gcctttatag tgtcattttg gaaggataat agaagtttgt
     2881 ggaatccagt attattttta ttatccctca tttcaagtta tttttatcta tcctaccttt
     2941 tttataaaaa tggatacgag aatgttcagt tagcttttta tgtatttgcc tttgtcttac
     3001 ttcctttttt gctttttcta agtggcatct ttttaatcta taatggggtc attttgttga
     3061 aacgagaggg acgttccaag ccccattatc tctcaatgct ctttgatttt tattgactat
     3121 tagattctat ttaaaaagac ttagaatgca catgaaagta tgtattctag gtcttttgtt
     3181 ttgtatacat ttaattttca agagtattat ctatttttcc atgcatggta acgagtatca
     3241 atcaataact gggtaaggcg acccaaggtt tctctttctt ctggagtgag ggattgagct
     3301 tggacaaaga gatgatgaat gtattcaagc gcctttaagg aagccaagtc attgatagag
     3361 ctaataccag catcaagaat agtgccaaag aagaggtcga agtagagctg aagttcttcg
     3421 tcagggactg tggccaccca ttctttaaag gttgtgtcta cttgccggct atcactgttg
     3481 gtcttatcca gttgaacgaa gtgcttgtcc tcaatctgcc aactaaaggt atcgtgctgg
     3541 gcgatgccac ccagggcagt actctgaacg atgatttggt gagcaggaat ttccagcatc
     3601 ataccgataa tggaaccttg tggaatgaat atcttgcttc tatccattat cctttgataa
     3661 cccgcagtct gtgtcaattc ttgatggaga ccaggtgcat caaatgtata aactgctgtg
     3721 atctgatttt gcaaactttg ctcaatttgg ctagcagcat agatagcgag atttcctccc
     3781 ttggaatgcc cagccagaat aaccttttgc ttaggatgat gggcaaaaaa gttctttaaa
     3841 tagcgaaggg cgtgcttttg agcaggaatt tccttcatat aggtcaggtg gaaatcttcc
     3901 ttccagccaa tgatactgtc atctgtccca cgaaagacaa tcagataggt atcgaggctg
     3961 acacgataag tcatagccgc aaattgcttt tgcagttcag ggtcgatgtc gttgataaaa
     4021 tgggagagtt tgcaattttt gaagcgcttg tgttgagcca attcatctaa taattgaagg
     4081 cgatttttgc tagtaagcat ggtgggctct cttggaacct gaggtgctag atctaaaaga
     4141 cgttgaggaa gtgtggagac cagattatca aaggagaggt aggtgatttc tattaaggtt
     4201 agaatgtcta actcattcaa gggaaggtcg taataagaat catatgcgac atctttcaga
     4261 tagtcaaaaa tattggccat aagagctcct ttctttttat ttatcttagc aaatttccct
     4321 caaattagct agtagcattg cctgtttgta ctggctaaaa acaggctatt tcaaattcag
     4381 tttcagacca tctggcatgg aaaaatctgt tataataata gaaaaggaga agcgcatgca
     4441 caagatttta ttaatagaag atgatcaggt cattcgtcaa caggtcggga aaatgctctc
     4501 tgaatgggga tttgaagtgg tcctggtaga agactttatg gaagttttga gtctatttgt
     4561 tcagtcggaa cctcatctgg tcctcatgga tattggtttg cccttgttta atggttatca
     4621 ctggtgtcag gaaatccgca agatttccaa ggtacctatc atgtttcttt cttcgagaga
     4681 ccaggctatg gatattgtca tggcaatcaa tatgggggcg gatgactttg tgaccaagcc
     4741 ttttgaccag caggttcttt tagctaaggt tcagggcttg ttgcgtcgtt cctatgagtt
     4801 tgggcgtgat gagagtttgc tggaatatgc tggtgttatc ctcaatacca aatccatgga
     4861 tttacattat caagggcaag tcttgaattt gaccaagaat gaattccaga ttttacgcgt
     4921 gttatttgag catgcaggca acatcgtagc acgtgacgac ctgatgcggg aactttggaa
     4981 cagtgacttt ttcattgatg ataataccct ctctgtcaat gtggctcgtt tgcgtaaaaa
     5041 gttggaggag cagggattgg taggatttat cgagaccaag aaaggaatag ggtacggatt
     5101 gaagcatgct tgattggaaa caattttttc tagcctatct gcgctcccgt agtcgtcttt
     5161 ttatctatct gctttctttg gcatttcttg tcttactctt tcagttttta tttgccagtc
     5221 taggaattta cttcctctac tttttcttct tgtgttgctt tgtaaccatc ttatttttca
     5281 cttgggacat attggtggaa acgcaggtct atcgccagga acttctctat ggagagaggg
     5341 aagccaagtc tcctttggaa atagctttag cagaaaaatt agaagcgcgt gagatggaac
     5401 tctatcagca gaggtcaaaa gcagaaagaa aactgacgga tttgctggat tactatacct
     5461 tgtgggtcca tcagataaag acccccattg cagccagtca actcttagtt gcagaagtgg
     5521 tcgaccgcca actgaagcag cagctagaac aggaaatttt caaaatcgac tcctatacca
     5581 acctagtttt acagtacctg cgtttagaaa gtttccatga tgatttgctc ttaaagcagg
     5641 ttcaaattga ggacttggtc aaggaaataa ttcgtaaata tgctcttttc tttattcaaa
     5701 aaggcttaaa tgtcaatcta catgaccttg ataaagaaat cgtgacggat aaaaagtggc
     5761 tgctagtggt tattgagcaa atcatctcaa acagtctcaa gtacaccaag gaaggtggtc
     5821 tggagattta tatggatgac caagagcttt gtatcaaaga tacgggaatc gggataaaaa
     5881 acagtgatgt cctccgagta tttgaacgtg gcttttcagg atacaatggc cgtttgaccc
     5941 agcagtcctc tggacttggc ctttatctat ctaagaaaat ttctgaagaa ctggggcacc
     6001 agattcgtat cgagtctgag gtcggaaaag gaacgacagt gcggattcag tttgctcaag
     6061 tgaacttagt ccttgagtaa aaatagatga gaaaaagcct ccagattgga ggcttttatg
     6121 ctagactctt atttctcaac gcgtgatttg ttggcgatat cagctaggat agcttgaaca
     6181 aaattatcaa ctgagacagt ttgtgtttct ttttggccgt agcgacgaac gttgactgtt
     6241 tcgtcttcca tttctttgtc tccaacaatt aattggtaag gaatcttgct ggtttgtgaa
     6301 gcacggatct tgaactgcat tttttcattg cgctcatcta cgtctgcacg gacaccgcgg
     6361 tcacggagtt tcttggccac ttcccaagcg tagtccacgt gtttttcgtt agatactggg
     6421 atgagggtta cttggtgtgg tgccagccat gttgggaagg cccccttgta gttctcaatc
     6481 aagatagctg tgaagcgttc catagttgag ataaccccac ggtggatcat gactggacgg
     6541 tgatcttcgc catcagctcc gatgtatttg aggtcgaagc gttctggcaa caagaaatca
     6601 agttggatag tagaaagggt ttcttctttt ccaagggcag ttttaatctg gatatccaat
     6661 tttggtccgt agaaggctgc ttcaccttcg gcttcaaagt agtccacgcc catttcatca
     6721 agagctgcac gaagcatggt ttgggcattt tcccacatct catcgttatc aaagtactta
     6781 tgagtatctt gagggtcacg aagagagagg cggaagcggt agtcagtcaa gttgaagtct
     6841 tcataaacat cgataatcaa ctgaagggca cgttggaatt cttcttggat ttgttctgga
     6901 gtaacgaata ggtgaccgtc gttgagtgac atttcacgta cacgttgaag gccagtgagg
     6961 gcaccagatt tttcgtaacg gtgcatcata ccgatttcag cgatacggat tggcaattca
     7021 cggtaagagt gaacatggtg tttgaaaact tggatgtggt gcggacagtt cattggacga
     7081 aggacaaatt cttccccgtc ccccatgtcc atggttggga acatgtcttc ttggtaatga
     7141 tcccagtgac cagaagtctt gtaaagctca acagaagcaa gtggtggagt gtagacgtgt
     7201 tggtagccag aagccaactc tttgtttacg atgtagcgtt ccaattcacg acggatagtc
     7261 gcaccatttg gcaaccagaa tggcaaacct tgtcccactt cttgtgaaat cataaagagg
     7321 tcaagctctt taccaagttt acggtggtca cgttccttag cttcttcacg catttgaagg
     7381 tagtttttca agtctttctt gtcaaaccaa gctgtaccgt agatacgttg catcatagcg
     7441 ttgtcgctgt ttccacgcca gtacgcacca gctacatgga gaaggtggaa gatttggata
     7501 cgacctgttg atggaacgtg aggtccacgg cagaggtcta catattcacc ctgacgatag
     7561 atagtcaaac cgccttcgtc ttctgagtgt tcttcaatca attccaactt gtaagggtca
     7621 tttttgaaga ttttacgtgc ctcgtcttta gtcacttctt cacgaataga tgggaagttt
     7681 tctttgacga ttttttgcat ttcttcttcg atacgaggaa ggtcttcgtt agagatttga
     7741 ccagctgtgt tgtcagtatc gtagtagaaa ccatcttcga tggctggacc aactcccaag
     7801 tgaatgtctg ggaaaagacg acgagctgct tgggcgaaca agtgagctgc tgagtgacgc
     7861 aagattggaa gggcatcttc gtgatcaggt gtcacaattt cgatgcttcc atcttcagtg
     7921 atagcgcgag tagtgtcgat gagtttgccg ttgaatttac cagccaaggc ttttttagct
     7981 agggaattgc tgatagattg ggcaatttca aaagttgtta cgccagattc gaattcacga
     8041 acagcgccat ctgggaaagt aatgttaatc atgatgttct ccttttttat tatttcatag
     8101 tttctaagca ttgatttttt atcatcagac tacttccaag tcctaacgtt caattgcttt
     8161 tctttatcag agaggaagtt ttccatttcc gtcagaatgt ttatatccaa acgttgagaa
     8221 agttctaata gccaccagat attttctgaa agtttttatt ccagtgtgta gggtgtttca
     8281 tcatagtagc gtccttgctt tgtcatcacc agtcgttgga aatttccaat atcattagat
     8341 aaagccaaga ggtcttcttc taccgtccac ttggaatcat gatgcttaac ttccagttcg
     8401 tgataagctt gtcggattgc ccaactgcgc tccactaatt cttgcaactc cataattagt
     8461 atcataataa aaaaatgcga catcctcgaa aggacgtcgc aacgtggttc caccttcatt
     8521 tatgtacctc aaaagagagg gacacctctg attggctcta acgtggccac cgttttatgt
     8581 tttcataaaa aactcaagag tagtatcagt ttagacttcc tatgcgtttc cagcaaccac
     8641 gcactctcta gtagaaaggg actaagtgac ttgtctctat ggattattat agcatgattg
     8701 tgagattttt caaggatttt gtgaaagttt tttgtttttc attttttgta gaatatgctt
     8761 gatgctaata ccagaatcca tgcccaccag agcaactgtt tcatagatta gcaagtaaat
     8821 tagtataaac tcaccaaagg ttatgatagt aaagtaaaag agcagtccgt taccaactaa
     8881 ccatcaaagt gagaaacgga aacggtagct atagagtaac gatacaatca agattaccag
     8941 aggtgtaaag aagagaagat tgtttatata gagtcccttg agtgtaaggg ggtcagaaac
     9001 aactagtaag aacaactcat aaagtggcca ataaaagaat aatatgacca gatagtaggg
     9061 gagatgggaa tagaatctac gcatgataat cacctcgttt aaaaaaaaca gacaacttag
     9121 atctcttgct atgattatta tagcatgttt aaaacaagaa agtaaatttg ttgacaaaga
     9181 agatttttct tttcactaga atcatggtta gttttcttgg tagaaatatt ttgataataa
     9241 ttaagcaaga ctattgacat tgattattaa gagtgtcaca attgttgtaa ctaaaataat
     9301 tacaacaaga gaggtataaa aaatgaaact agcagttatt gcagcaaatg gacaagtggg
     9361 gaaggcaatt gttgaagaag cagtcaaacg aggacatgaa gtaacggcta ttgttcgttc
     9421 tgaaaacaaa agtcaagctg agtctattat taaaaaagat ttatttgagt tgacaaaaga
     9481 tgatcttact ggatttgatg ctgttatttc agcgtttggt gcttatacac cagatacgct
     9541 accgctccat agcaaatcaa tcgaactatt taatcagctt cttgctggaa cacagacacg
     9601 ttttctagtt gtagggggag caggaagtct ttatatagat gaaacaaaaa cgacacgact
     9661 tttagatacg cctgattttc ctgaagaatt taagtcacta gcaaaagctc aagcggatga
     9721 attggatctt cttcgtacaa aaaataatct caactggact tttgtcagtc ctgctgtaga
     9781 ttttattccc gatggtgaaa aaacaggaaa ctacattcta gcaggagaaa tttttactac
     9841 taatgaaaaa ggaataagcc agattagtta tgcagattat gctattggat tggtagatga
     9901 acttgaaaaa ggtcatcata taaaagaacg tatctcttta cttgaaaaat aataactatg
     9961 agagtattaa tgtttgacag aaaatttaaa tcttggtaaa atagggattg tcgtaaagac
    10021 aaataacttc ttcttggtta caggcatgcc aacctgtcac tcggatgaag ccaaataaaa
    10081 aggagaaaca tcatggcaat ctcaaaagag aaaaaaaatg aaatcattgc acaatatgca
    10141 cgtcacgaag gtgatacagg ttcagtagag gttcaagttg ctgtccttac ttgggaaatc
    10201 aaccacctta acgaacacat caaacaacac aaaaaagacc atgctactta ccgtggattg
    10261 atgaaaaaaa tcggtcgccg tcgtaacttg cttgcatact tgcgtaaaaa cgacgttaac
    10321 cgttaccgtg agttgatcaa ctctctagga cttcgtcgct aattcaagat acaagtgctt
    10381 aggccgtgtt caattgagca tatcttgata atgaagctac tctaaattgg gtagcttttt
    10441 tgcgttgtca ccttacctcg ctatactcaa gtataatctt tggttacggt tcctagcact
    10501 gtaaggtaaa ataaaccagc ttttctccct tcggggagat tttttttgtt tcactgaaag
    10561 tttcgtacga tcttcgcctc gtcgcctagt ctataaacga tttatctagc aagaatcatg
    10621 atgctaaggg cgtcaaaaat ccgtatgaaa atagggaaat gacacagtgt tcgatgagca
    10681 caaggagttt catctttttc actaggattt tagcccgggc tcaaatcagc tctctgactt
    10741 cagagggctt ttttattgag gttttatcgg taacaatttt ggagactaca aaaacctcaa
    10801 acggtatcag ctaattacac cataaaggtg cgcagttact cggcttttca agccgagtag
    10861 ctgtatgcaa gccccctaga agagcccact ctttacaaag taaagtatag tatgttatac
    10921 tttacatgga agtagtcact gaattccagt tagaaattac tttgtaacta cgttttgagg
    10981 aggagtaaaa tgctttccta cgttcgacat tacccactag cgatagctaa attaatgtgt
    11041 ctgtgctctc ctaaaatctg ctgatttatt actgactaat acaggagttt tttttatggg
    11101 acagacaatc atatctgcta ttggtgttta tatttccacc agtatcgatt atttaattat
    11161 tttaattatt ttatttgcac agctatcaca gaataaacag aaatggcata tttatgcggg
    11221 gcaatatcta ggcacaggct tacttgtagg ggcgagttta gttgctgctt atgtcgttaa
    11281 tttcgtgcct gaagaatgga tggttggatt gcttggttta atccctatct atttagggat
    11341 tcgctttgca attgttggag aagatgcgga agaagaagag gaagaaatta ttgaaagatt
    11401 agaacaaagc aaggcaaatc aactgttttg gacagttaca ttgctgacaa ttgcgtctgg
    11461 cggagataat ttaggtatct atatacctta ttttgcttcg ttagattggt cacagaccct
    11521 cgtggccttg cttgtgtttg taatcggcat aattatcttt tgcgagatta gtcgggtgtt
    11581 atcctctatt ccgttaatat tcgagacaat tgaaaaatac gagcgaatca ttgtgccctt
    11641 agtattcatt ctacttggac tatacatcat gtatgaaaat ggcacgatag agacttttct
    11701 gatcgtgtag atttttttgt ttcactagga ttttagcccg agctcaaatc agctctctga
    11761 ttttcagagg gctttttcat tgagtataaa tggacaatct taaagttact ctattgtaga
    11821 gtggcttttg atttgattag gagattctta taaatatagt aaaatgaaac aaaaacagga
    11881 caaatcgatc aggacagtca aatcgatttc taaaaatgtt ttaagaagta gaggtgtact
    11941 attctagttt caatttgata tataaatgaa aaccttagct gtttggccaa ggttcatttc
    12001 ttctatgcaa gttcttctct tttcttatct gggttccaga tgaagcttgc gataaagcta
    12061 aagatgatgg ttaggatagc gaggataata gcaaggatga gggcagggat gcggataaac
    12121 caccagagtg gatttggttt tttatcattg tgccattgtt tcgtttcttc gtccagacgt
    12181 tggaattctg tttgaatacg attggcgact gtttcaatgc cttcatcatt cattttcttg
    12241 atatctgaga tatcgattgg atttccaaag ttcatatcga cacgttcacg gctaatcaag
    12301 cccttcaaag tcatgggacc ggtgtaggta accggcatga tacggacctt ggccattttg
    12361 gcaatcagtg ctgcgccccc cttgacatcg tttgagtggc ggctaccact tggaaacatg
    12421 atgagagagc ggtcactttt tttgagaacg ttgataggat atttgatggc tgaggcgctg
    12481 ggattttcac ggtcgatggg aaaggcgcca cacatacgaa tccaccaacc aaagatacgg
    12541 ttggtaaaga gttctttttt tgccataaag atgaactgtt ttggcttggt cgcaaaggcc
    12601 atataaacag gatcccacca ggtacggtga ggcgcaacta aaatataatt ttcatcttga
    12661 ttaggaattt tatcagtatt atgatagtga gcattgccat tgatggacca taagagcaat
    12721 acaactaatc cacgcaaata agtataaaac atgcgatctc cttcgattgt tttcttgtta
    12781 ttattatacc ttatcaaagg agggctggca aacttttccc ttgactagat gcatatttgg
    12841 gatgaaatta gaattctgtt aaaaaaaatg atataataga atttatggat aaaaataaga
    12901 ttatgggatt aacccaaaga gaagtcaagg aaagacaggc tgagggtttg gtcaatgact
    12961 ttaccgcatc agccagtacc agcacttggc aaatcgttaa acgaaatgtc tttacccttt
    13021 ttaacgcttt gaactttgcc attgctttgg cccttgcctt tgtgcaggct tggagcaatc
    13081 tggtcttctt tgctgttatc tgctttaacg ctttttctgg gattgtgacc gagctacgag
    13141 ccaaacacat ggtggacaag ctcaatctca tgaccaagga aaaggtcaaa accatccgtg
    13201 atggtcagga agttgctctt aatcctgaag aattagtgct aggagatgtc attcgtttgt
    13261 ctgcaggaga gcagattcct agtgatgcct tggttttgga aggctttgcg gaagtcaatg
    13321 aagccatgtt aacgggagaa agtgatttgg tgcaaaagga agttgacggc ttacttttgt
    13381 caggaagttt cctagccagt gggtcagttt tatctcaagt tcaccatgtc ggtgcagaca
    13441 actatgctgc caaactcatg cttgaggcta agaccgttaa acccatcaac tcccgtatca
    13501 tgaaatcgct ggacaagttg gctggtttta ctgggaagat tatcattccc tttggtctgg
    13561 ctctcttgct ggaagccttg cttttaaaag gcctgcctct caagtcatcc gttgtaaact
    13621 cgtcgacagc tcttttggga atgttgccta agggaattgc ccttttgacc attacttcgc
    13681 tcttgactgc agtgattaag ttgggcttga aaaaggtctt ggtgcaggag atgtactctg
    13741 ttgagacctt ggcgcgcgtg gatatgctct gtctggacaa gacgggcacc atcacccaag
    13801 gaaagatgca ggtggaggct gttcttccac tgacggaaac ttacggtgaa gaggctattg
    13861 ccagcatctt gactagctac atggcccata gtgaggataa gaatccaact gcccaagcca
    13921 ttcgccagcg ttttgtggga gatgttgctt atcctatgat ttccaatctt cccttctcca
    13981 gcgaccgcaa gtggggggct atggagttag aaggcttggg gacagttttc ttaggggcac
    14041 ctgagatgtt gctggattct gaagtcccag aagccaggga ggccttggag agaggatcac
    14101 gtgtcttggt cttagctctc agtcaggaga aattagacca tcacaaacca cagaaaccat
    14161 ctgatattca ggctctagcc ttgctggaaa tcttggaccc tattcgagag ggagcagcag
    14221 agacgctgga ctatctccgt tctcaggagg tgggactcaa gattatctct ggtgacaatc
    14281 cagttacggt gtccagcatt gcccagaagg ctggttttgc ggactatcac agctatgtag
    14341 attgctcaaa aatcaccgat gaggaattga tggccatggc ggaggagaca gctattttcg
    14401 gacgtgtttc cccccatcaa aagaaactca tcatccaaac gttgaaaaaa gcgggtcata
    14461 caacggctat gacaggggac ggggttaatg atatcttggc ccttcgtgag gcggattgtt
    14521 ctatcgtgat ggcggagggg gatccagcaa cccgtcagat tgccaatctg gttctcttga
    14581 actcagactt taatgatgtt cctgagattc tcttcgaggg tcgtcgcgtg gtcaataaca
    14641 ttgcccacat cgccccgatt ttcttgataa agaccatcta ttccttcctg ttagcagtca
    14701 tctgtattgc cagtgcttta ctaggtcggt ctgaatggat cttgattttc cccttcattc
    14761 caatccagat taccatgatt gaccagtttg tggaaggttt cccaccattc gttctgactt
    14821 ttgagcgaaa tatcaaacct gttgagcaga atttcctcag aaaatccatg cttcgtgccc
    14881 taccaagcgc tctcatggtc gtctttagcg tcctgtttgt gaaaatattt gggagtagcc
    14941 aaggttggtc tgagttagaa atctcaactc tcctctatta tctcttgggg tcaattggtt
    15001 tcttatccgt atttagagcc tgcatgccat ttaccctatg gcgtgtcctc ttgattgttt
    15061 ggtcagtagg aggtttccta gccacagctc tcttcccaag aatccaaaaa ctgcttgaaa
    15121 tttcaacctt aacaggacaa acattacctg tttatggtgt catgatgttg gtctttaccg
    15181 tgattttcat cctgaccagt cgctatcaag ccagaaaata aagaaagact gcaatctgtg
    15241 gattgcagtc tttttaggta caagattgcc agccgaaatg tggtataata agaggtaata
    15301 gagttttgga aagtgagaga agatgatttc aaagagatta gaattggtag cttcctttgt
    15361 gtcacagggg gctattttac tagatgtggg aagtgaccat gcttatctgc ctatcgagtt
    15421 ggttgagaga ggccaaatca aaagcgctat tgcaggtgag gtggtggaag gtccctatca
    15481 gtctgcggtt aaaaatgttg aggctcacgg cctaaaggag aaaatccaag tccgtttagc
    15541 caatggcttg gcagcttttg aagagactga ccaagtgtct gtcattacca ttgctggcat
    15601 gggtggtcgt ttgattgcta ggattttaga agaaggtttg gggaagttag ctaatgtaga
    15661 gcgtttgatc ctccagccca ataatcgtga agacgacttg cgtatctggc tacaggatca
    15721 tggattccag attgtagcag aaagaatctt agaagaagct ggaaagtttt atgagatttt
    15781 ggtggtggaa gcaggacaaa tgaagctatc agccagtgat gttcgctttg gtcccttctt
    15841 gtccaaagaa gtcagtccag tatttgtcca aaaatggcaa aaagaagctg agaagctaga
    15901 gttcgccctc ggacaaatcc cagaaaaaaa tctggaagaa cgtcaagttc tagtagataa
    15961 gattcaagct atcaaggagg tgctccatgt tagcaagtga agtgatccaa gcttatgaag
    16021 ccttttgccc tcaggaattt tctatggagg gagacagccg tggtctgcaa attggcactt
    16081 tagacaaggg tatccaaagg gtcatggttg ccctcgatat tcgtgaagag acggtggcag
    16141 aggccattga aaagggtgtg gacttgatta tcgtcaagca cgcgcctatc tttcgtccta
    16201 tcaaggactt gcttgccagc cgtccgcaaa atcagattta catcgacctg attaagcatg
    16261 atatcgcagt ttatgtcagc cataccaata ttgatatcgt tgaaaatggg ctcaatgact
    16321 ggttttgtca gatgctagga atcgaggaga cgacttatct gcaggaaaca ggtccagaac
    16381 gtggaattgg acgtattggg aatattcagc ctcagacatt ttgggaattg gcccaacagg
    16441 tcaagcaagt ctttgaccta gatagccttc gaatggtgca ttatcaagag aatgatttgc
    16501 agaagcctat ttcaagagta gcaatttgtg gtggaagcgg gcagtctttc tataaggatg
    16561 ctttggcaaa gggggcagat gtctatatca ctggcgacat ctactaccat actgctcagg
    16621 atatgctgtc tgatggcttg ttagcattgg atccaggtca ctatatcgaa gtgctttttg
    16681 tggaaaaaat cgcagcactc cttactcaat ggaaagagaa aaaaggctgg gaactagaga
    16741 ttttacctag tcaagcatcg accaatcctt tccgtcatat ctagttagaa agtgaagaca
    16801 atgaaaaaag ttgccattat tggagcagga attgtgggag caacagctgc ctactacctc
    16861 tcgagagaaa gtgacctaga ggtgaccgtt tttgaccatg agcaaggtca agccaccaag
    16921 gccgcagcag gaattatcag tccttggttt tccaaacgcc gtaataaagc ctggtacaag
    16981 atggcgcgct tgggggctga tttttatgtg gatttattag ctgatttaga gaaatcagga
    17041 caagaaatcg acttttacca gcgttcggga gtctttctct tgaaaaagga tgaatccaag
    17101 ttggaagaac tctatcaact ggctctccag cgcagagaag aatctccctt gataggccag
    17161 ttagccattc tggatcaagc atcagctaat gaattattcc ctggcttgca gggattcgac
    17221 aacttgctct atgcttctgg tggagcgaga gtagatggtc acctcttagt aactcgtttg
    17281 ctggaagcca gtcaggtcaa gttggtcaaa gaaaaagtga ctctgacact cttatcatta
    17341 ggctaccaga ttggcgaaga ggtgtttgat caggttattt tggcaacggg agcttggttg
    17401 ggggacatgt tagaaccttt aggttatgaa gtggatgtcc gtcctcaaaa aggacaacta
    17461 cgagattatc agcttgccca agacatggaa gattaccctg ttgtcatgcc agaaggggag
    17521 tgggatttga ttccctttgc aggtgggaaa ttatccttgg gcgctaccca tgaaaatgac
    17581 atgggatttg acttgacggt agatgaaccc ttgctccaac aaatggagga ggcggccttg
    17641 cctcattacc cagttttagc tagagtgact tcaaaatctg agcgtgttgg aatccgtgcc
    17701 tacaccagtg atttctctcc tttctttggg caggtgcctg acttaactgg tgtctatgca
    17761 gccagtggac taggttcatc aggcctcaca actggtccta ttattggtta ccatttagct
    17821 caactgatcc aagacaagga gttgaccttg gaccctgaaa actacccaat tgaaaactat
    17881 gtcaaacgag taaaaagcga ataatactca atgaaaatca aagaacaaac taggtaacta
    17941 gccgcgggtt actcaaagca ctgctttgag gttgtggata gaactgatga agtcagtaac
    18001 catacccatg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga gtataagcat
    18061 tttactgaaa ttttatcagg tagtttagga tggcaaatga cattccctgt caaaaatggt
    18121 aaaataagaa aaaataatct gagaatcgag gaaaaaagat gcaagaaaag attttggtaa
    18181 ctggtggtgc cggttttatt ggaacccaca ctgttattga attgatccaa gcaggccatc
    18241 aagttgttgt ggtggataat cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa
    18301 gaatcacagg agttgagatt cctttctatg aggcagatat tcgtgacaca gataccctca
    18361 gagatatttt caagcaggaa gaaccgactg gtgtcattca ctttgctggt ttgaaggctg
    18421 ttggcgaatc aacacgtatc cctcttgcct actatgacaa caatatcgct ggaactgtca
    18481 gccttttgaa agtcatggaa gaaaacaact gtaaaaacat catcttcagt tcttctgcga
    18541 cagtttacgg ggatccgcac acagtgccca tcttggaaga tttcccactt tcagtgacca
    18601 acccatacgg tcgtactaag ctcatgctag aggaaatttt gactgatatt tacaaagcag
    18661 actcagaatg gaatgttgtc ttgcttcgtt actttaaccc aatcggagcc catgagagtg
    18721 gtgatttggg agaaaatcca aacggtattc caaacaatct cttgccatat gtgactcaag
    18781 tagccgttgg aaaattagag caagtgcaag tgtttggaga cgattacgat acggaagatg
    18841 gaacaggtgt tcgtgactat atccacgttg tcgatttggc taagggtcac gttgcagctt
    18901 tgaaaaaaat ccaaaaaggt tcaggactaa acgtttataa ccttggaact ggtaaaggtt
    18961 actcagttct tgaaattatc caaaacatgg aaaaagcggt gggacgtcct attccttacc
    19021 gcatcgtaga acgtcgccca ggtgatatcg ctgcctgcta ctcagaccca gcaaaagcta
    19081 aagcagaact cggttgggaa gcagaactcg acatcaccca aatgtgtgaa gacgcatggc
    19141 gttggcaaag caagcatcca aatggatttg aagactaaga tgatgatttc aatcatcgtc
    19201 ccttgtttaa acgaagagga agtacttcct cttttttatc aggctctgga agctttactt
    19261 ccagatttgg aaacagaaat cgagtatgtc tttgtcgatg atggatcaag tgatgggacc
    19321 ttggaactct taaaggccta tcgggagcaa aatccggcag tccattatat ttctttctct
    19381 cgaaattttg gcaaagaagc agccctttat gcaggcttgc aatatgcgac aggagatttg
    19441 gtggtggtga tggatgcaga cctccaagat cctcctagta tgttgtttga gatgaaaaat
    19501 gtactagaca aaaatgtaga cttggactgc gttgggacac ggagaattag tcgggaggga
    19561 gaacccttct ttcgcagttt ctgtgctgtt ctcttttatc gcctcatgca aaaaatcagc
    19621 ccagtagctc tgccgtcggg tgtccgtgat tttcgtatga tgagaaggtc tgtggtcgat
    19681 gccattttaa gtttgactga gtccaatcgt ttttctaagg gactctttgc ctgggtcggc
    19741 tttaaaaccc actatctgga ctatccaaat gtcgaaaggc aggctggcaa gaccagttgg
    19801 agttttaggc aactcttttt ctactccatt gaagggatta ttaatttttc agattttccc
    19861 ttgattatag cctttgtagc tggtctccta tcttgttttc tttctctgct gatgaccttt
    19921 tttgttgtgg ttcggaccct cattttgggc aatccgacat cgggttggac ctctctgatg
    19981 gctgtcattc tctttcttgg aggcattcaa ctcttgacca ttgggattct cggcaagtat
    20041 atcagtaaga tttatttaga gactaaaaaa agaccacttt atcttatcaa agaaaaaagt
    20101 gaccttcctg attttacaga aaaaaataaa gtgaaaagac tataatttta catggaaatg
    20161 tgctaaacta gagggagtgg attatcgcca ttgatttagg cttcctggtt tccaatcagg
    20221 gcgcgagttg ggcaattttt aacagcctct aagacatcct gactaggaga aatttctttt
    20281 tccagttggt cagggtcatc gtaaaaacgc acgattccat tatcgtggta atcaaataaa
    20341 tcagaataag tttggcaaag cccacaggcg atacatcgtt caggtataag tgtgattttc
    20401 atgttcatat ttatattgta ataagaaaag taaaaaaaaa caaggagtaa ggtatggcaa
    20461 aagaaccgtg gcaagaagat atctatgatc aagaagaatc aagagcagag cgtcggcatc
    20521 gaaaccacgg aggggctgat aggatggcta atcgtatttt gacgatccta gctagtattt
    20581 tctttgtaat tgtggtggtg atggtcatcg ttctcatcta tctatcatcg gggggaagta
    20641 atcgcacagc agccttaaaa gactttcatg attctgatgc aagtgtagta caaatctcat
    20701 cttcaagcag ttctcagcct gagcagagtt cagagccaga atctacttct agtagttcag
    20761 aagaagctgc taatcctgaa ggaacgatta aagttctcgc aggagaaggg gaagcagcta
    20821 ttgccgctcg tgcaggaatc tccattgctc agttagaggc cttgaatcct gggcacatgg
    20881 ctacaggatc ttggtttgct aatccaggtg atgttataaa aataaaatag gagccgatat
    20941 gaaaacaatt caaattgcta ttgatggtcc tgcttccagc ggtaagagta cggtcgcaaa
    21001 gattattgct aaggattttg gattcaccta ccttgataca ggagctatgt atcgtgcagc
    21061 gacctatatg gctcttaaga accaattagg agttgaagaa gtcgaagccc ttctagcctt
    21121 gttggaccag catccaatca gctttggacg ttcagaaact ggagaccagc ttgtttttgt
    21181 aggagatgtg gatattaccc atcctatccg tgaaaatgaa gtgaccaatc atgtttctgc
    21241 tattgcagca attcctgaag tgcgtgagaa actggtttct ctccaacaag aaattgccca
    21301 gcaaggcggg attgtcatgg atggtcgcga tattggaact gttgtattgc cacaagcaga
    21361 attgaaaatt ttcctagtag cttctgttga tgagagagca gagcgtcgtt acaaggaaaa
    21421 tattgccaag ggaattgaaa cagaccttga aaccctaaaa aaggaaattg ctgcgcgtga
    21481 ctacaaggat agtcatcgtg agacttctcc tctcaaacaa gcagaggatg ctgtctacct
    21541 tgatacaact ggtttgaaca ttcaagaagt agttgaaaaa atcaaagcag aagctgaaaa
    21601 aagaatgtaa atgtagaaaa gccgatagat tgatatcggt ttttttctaa atttgtcaaa
    21661 atgctgattt tagggtataa tagttgctgt tcgagaaatt ttgattttca aagaatagtg
    21721 aatgatgata aggagaaatc atgaacaatt taccaaattg tccaaaatgt aactcagagt
    21781 atgtctacga agacggtgct ctactggttt gcccagagtg tgctcatgag tggaatcctg
    21841 ctgaagttgc agaagtagaa gaaggtcttg tcgctatcga tgccaacgga aataaattga
    21901 ctgatggtga tacagtaact ctcatcaagg acttgaaagt aaaaggtgcg ccaaaagatt
    21961 tgaaacaagg gacgcgcgtg aaaaatatcc gcatcgtaga aggcgatcac aatatcgact
    22021 gtaagattga tggttttggt gccatgaaac ttaaatcaga gtttgtgagg aagatttaag
    22081 catgtcaaaa cactataaac ttgtatttta tagccgtatc ttcttgtttc tagcggcttt
    22141 tacgggagtt tatcttgaaa ttactaggca tggtggtttt gggatgcttc tctattacac
    22201 ggttctgtcc aacctcttgg taactatttt taccctttat cttctaaagg ttatgagccg
    22261 tgtaggtgaa aattggcaaa ggccaagtct cttgcgctta aaaggtgggg tcaccatgag
    22321 tatcatgatt acctgtgtga tttaccattt cctcttggcg cccattgcga ctaatttcta
    22381 taccctagaa aatttccttt gccactatat cgttcccatc tggtttttag cggataccct
    22441 cttttttgac aaacagggtc aatacaagat ttgggatcca gcagtgtgga ccattttacc
    22501 ctttctgtat atgatgtttg ctctttttaa tggcttggtt ctaaaactca atattccaaa
    22561 tgccaaggat aatcctttcc cttacttctt tttgaatgtg aacaagggtt ggaatgttgt
    22621 gtttaagtgg tgtctgatta tctttgttgc ctatatggta gcaggattta ttttctactt
    22681 tatcaagcaa atcaagagaa agtcatccta atactcttcg aaaatctctt caaaccacgt
    22741 cagtttccat ctgcaacctc aaaacactgt tttgaacaac ctgcggctag cttcctagtt
    22801 tgctctttga ttttcattga gtataagata caagggattc attttcgaat ttaagaagga
    22861 gtctacaaac caatgaggct tcttcttttc ttgcttattt gataactatc aaaaaagtag
    22921 aagattaaga gaaatataga aagagatttc atgaaacaat ttttagaacg ggccagcatt
    22981 ttggctctct ccctcgtttt gattacctcc ttttccattt cgagtgccct accagccatg
    23041 tttgactatt atcagggtta ttctaaggaa caaattgagc tcttggtgag cttgccttcc
    23101 tttggaatca tgatgatgtt actgctaaat ggtttcttag aaaaaatatt tcctgagcgc
    23161 ttacagatta gtttgggctt gctgatttta tcattgagcg gtacagctcc cttctggtac
    23221 caagcctatc cctttgtctt tggaacacgg cttctctttg gtttgggtct tgggatgatc
    23281 aatgccaagg ccatttctat tatcagtgaa cgctaccaag gaaaaaggcg aattcagatg
    23341 ttagggctac gcgcttctgc agaggtcgtt ggagcttctc tcattacctt ggccgtcggt
    23401 cagttgttgg cctttggttg gacagctatc tttctagcct atagtgctgg atttttggtg
    23461 ctgccccttt atctgctctt tgtcccttat ggaaaatcaa agaaagaagt caagaaaaga
    23521 gcgaaggaag caagtcgttt aactcgagaa atgaaaggct tgatttttac cttagctatc
    23581 gaagcggcag ttgtagtttg taccaataca gctattacca tccgtattcc aagtttgatg
    23641 gtggaaagag gattggggga tgcccagtta tctagttttg ttcttagtat catgcagttg
    23701 atcgggattg tggctggggt gagtttttct ttcttgattt ctatctttaa agagaaactg
    23761 ctcctctggt ctggtattac ctttggcttg gggcaaattg tgattgcctt gtcttcatcc
    23821 ttgtgggtgg tagtagcagg aagtgttctg gctggatttg cctatagtgt agtcttgacg
    23881 acggtctttc aacttgtctc tgaacgaatt ccagctaaac tcctcaatca agcaacttca
    23941 tttgctgtat taggctgtag tttcggagcc tttacgaccc catttgttct aggtgcaatt
    24001 ggcttactaa ctcacaatgg gatgttggtc tttagtatct taggaggttg gttgattgta
    24061 atctctatct ttgtcatgta cctacttcag aagagagctt aggattgatt cctaagtttt
    24121 tcttttgata ctcaatgaaa atcaaagagc atagttgatt gagtttggaa tagtacgctg
    24181 tagttgctaa aatatttcta gaaattaatt tgacttttct aatagagtta ttcatatctt
    24241 atttcaatct actatagaaa gctagccgca ggttgctcaa aatagtgttt tgaggttgca
    24301 gatggaaact gacgtggttt gaagagattt tcgaagagta tgagattttt atacccatac
    24361 aatatttgtt ttaggtcctt gtttacagac taatttcttg ataaaataga ggttatgacg
    24421 agatataaag caactatttc ctatgatggt tatgcctttg ctggctttca gcgccagtct
    24481 catgcgcgta gcgttcagga agaaattgaa aaaaccttga ccagattaaa taaagggcaa
    24541 accattactg ttcacggtgc tggtaggaca gatagtgggg ttcatgccct gggacaggtc
    24601 attcattttg acctgcctta tcagatggat gaggagaaac tccgttttgc cttggatacc
    24661 cagtctcctg aagatattga tgtgatttcg attgagcttg tggcagatga ttttcattgc
    24721 cgttatgcca aacatagcaa gacctatgag tttattgtgg atagaggacg tcccaaaaat
    24781 cctatgcgcc gtcactatgc cacccacttt ccctacccac tcgatgtgga acgaatgcag
    24841 attgcaatca aaaagctaga gggaacccat gattttaccg gttttacagc ctctgggact
    24901 agtgtagagg ataaggttcg caccatcaca gaagctagtt taatagtcga tgagacagga
    24961 caatttttga cctttacctt ttcaggaaat ggtttcttgt ataaacagat tcgcaatatg
    25021 gtggggactc ttctcaaaat cggtaacaac cgcatgccag tagagcagat tgatctcatc
    25081 ttggagaaga aggacaggca acttgcaggt cccactgcag caccaaatgg tttgtattta
    25141 aaggagattc gttatgaaga ataatcgtat tttagcactt tctggaaatg atatttttag
    25201 tggtggtgga ctgtcagctg atttggctac ctataccttg aacggcttgc atgggtttgt
    25261 agcagtgact tgtttgacag ccttgacaga aaaaggattt gaagtctttc caactgatga
    25321 taccattttt caacatgaat tagatagctt gcgtgatgtg gaatttgggg gaattaagat
    25381 tggtcttctc cctactgtca gtgtggctga gaaggccttg gactttatca aacaacgccc
    25441 aggagtacct gtggtgttgg atcctgtctt ggtctgcaag gaaacgcatg atgtagctgt
    25501 cagtgagctc tgccaagagt tgattcgctt tttcccttat gtcagtgtga ttacgcctaa
    25561 tctcccagaa gcagaattat tatccggtca ggaaattaaa accttggaag acatgaaaac
    25621 tgcagcgcag aaattgcatg atttaggagc gccagcagtc attatcaagg gaggcaatcg
    25681 tcttagtcag gacaaggctg tggatgtctt ttatgatgga cagaccttta ctatcctaga
    25741 aaatccagtt atccaaggcc aaaatgctgg tgcaggttgt acctttgcct ctagcattgc
    25801 cagtcacttg attaaaggtg ataaactttt gccagcagta gaaagctcta aggctttcgt
    25861 ttatcgtgct attgcacaag cagatcagta tggagtaaga caatatgaag caaacaaaaa
    25921 caactaaaat cgcccttgta tccctattaa ccgccctttc tgtggttcta ggttatttct
    25981 taaaaatccc aacacctaca ggaattctaa ctcttttaga tgctggtgtc ttctttgcgg
    26041 ccttttactt tggtagtcgt gaaggagcgg tagtcggagg actagcaagt ttcttgattg
    26101 acctcttatc aggctaccct cagtggatgt tctttagctt ggtcaaccat ggcttgcagg
    26161 gatttttcgc aggatttaaa ggaaaaagtc agtggttagg ccttatttta gcaactattg
    26221 ccatggtagg aggctacgcc ttgggttcta ctttgatgaa tggctgggca gcagccctcc
    26281 cagaaattct accaaatttc atgcaaaata tggtagggat gattgtagga tttattctta
    26341 gtcaaagtat caagaagatt aagtaatact cttcgaaaat ctcttcaaac cacgtcagtt
    26401 tccatctgca acctcaaaac actgttttga gcagcctgcg gctagctttc tagtctgatt
    26461 ttcattgagc ataaagaggt tgagtaaaaa gtctttcaaa taagaaaaac gcatagtatc
    26521 aggtgttgaa tgaacacctg atactatgcg ttttattgtg ggaagatttt cctcattttc
    26581 tagtgaaatt gaactttttc taacatcttt tgatagatga cttcttaatc aattatcatt
    26641 tccattgttg tttcttgggg tttcatccct tgtctttgat aaaagcggag agcaccctcg
    26701 ttatcattcc agacacttag ggttagatta ttgcagccaa tttctttagc ataatagcag
    26761 aaaaatgaca ataaaatagc ccctagatat tttgaaagga aagcataggg gctatatagt
    26821 ttaatcaaaa taaagcaaat ctttgctggt gcttgtaaag aggtcgaagc catccttggt
    26881 aacaacaccg cagtcttcaa tacgaacacc gactttacca gggatataga tacctggttc
    26941 aacagagaag cacatgcctt cttcgatgac catgtcgttt ccttccatga tagatgggaa
    27001 ttcatggaca tccataccga taccatgccc gagacggtgg ttgaagtact caccataacc
    27061 agctttttcg atgacctcac gggcagcgcg gtccacttca tgagcagtca cacctggctt
    27121 gataaagtca agagcagctt gttgggcttc aagagtcaag ttgtaaatat ctttcttgaa
    27181 ttggtctggt ttgccgacag cgactgtacg agtcatatct gacgcatagc cattgaccag
    27241 aacacccagg tcaaagagga gaagagcatc attttcaacc ttattagctg ctggaatgcc
    27301 gtgtggattc gcagcattat caccagtcaa gaccatggta tcaaagctca tttcgtaacc
    27361 ttcacgtttc atggcaaagt caatttgtgc gatgatatct gtctcagtct tatcaagaga
    27421 aatattgtca aaaccaacat gaacagcctt gtcagcataa agacctgcaa ccatcatttt
    27481 ttgcacttca tcagctgatt tgatgaggcg catgcgttgg atacgaggag tgaggttgtc
    27541 aaactcagca gtctcaaaaa ctgttttcaa accatggtat ttggtcaaga tgagattgtc
    27601 aaactcaaca gcgacacgtt tgaagtcaag ttgtggaaga gcatgtttga ttttttgcca
    27661 tggattttca gaatcgacat agcccactac tgggaaggaa acggtgctac ttgcacgttc
    27721 tacttcaaga gctgggacaa agaggagagg ttcctgatct gctaggacaa agaggaacat
    27781 ttggcgttca tggggatcac tgtaaaaacc agtgaggtaa ttgattgtga cggggtcaga
    27841 tacgacagcg acgtctagtt tttctgattc aagatatgtt aggatttgtt gtaatttaga
    27901 catatgctac cttctttcta tccctctatt ttggcaaaaa atgagagaaa atgcaaggga
    27961 gaagggtaaa agaaaagaca aaaaactccg acaagataac ggagttcttt gatatcattt
    28021 tcctttgttt aagaaaagtc agccttgctt ttgacgtcac tgtactcttc agcgatttct
    28081 tggctgagga tatcctcata ggcagggagt tggatatcct gaccatattt cttcttgagg
    28141 gcagtagtaa ctaggcgata agccagattg gcattacgag agaggatagg cccgtggaag
    28201 taggaaccaa agacattctt ataatgaacc ccttcaccga ccttttcttc gttgtttcca
    28261 tttccataga caacctgtcc cagcggtttt tggtcatcag agaggaaggt acggccttgg
    28321 tgattttcaa atccatagta ggtttcatcg aaatcttcat tgtgaatctt gatgtcaccg
    28381 ataaaacggt tattggtctg gttgagcgtg tagtgtccca tgacccctag cccttcgata
    28441 cgttttcctg aagcttcaac ataatattga cccaatagtt ggaaaccacc gcagatagcc
    28501 agaactacac cgtcgttttg gatgtagttg tcaatgctct cttttttagc aggtaggtcg
    28561 tctgcaatga tactttgttc aaagtcttga ccaccaccga aaaaggcgat gtcgtagtga
    28621 ttttcatcaa agtcatcatg gagagaaacg atgtcaacgg tcacatgggc tcccagtttt
    28681 tcagccacat acttgagcat gaggatgttt ccattgtccc cgtaggtatt catgagattt
    28741 ccgtagaggt gggcaatgtt gagctgatag gggtaattgc catcttttga ggaaagtgaa
    28801 gtataaacca ttagttcatc tcctttctaa caatctgacg actagccagc agttcacgaa
    28861 attccagcat ggcagtataa gttgccagaa tataggcatg cttgcagtct tgattctcaa
    28921 tggtcttgag aacttgctcc agattactcg tttcagtgat tttctcagct ggatagccag
    28981 tcactcggag gcgacgagcg atttcagaat gacgaacacc gccagcgttg atttcaggaa
    29041 tgtccatgtc agtgatttgt tcaaagtctg catcccagat ccagctagtg tcaattccat
    29101 ctgcatagtt ggcattaagg aggacagata ggctaaatgg ataaggtgct agtttgatca
    29161 tttcgatagc ttgggttgca ccgactggat ttttaatcaa gacaagggta cattccttgt
    29221 caccgatatg aaaggtttct tggcgtccaa agacagcacg gctcttgtca aatccctgtt
    29281 tgatgagttg cgaatcggca cctaggaaac gggcgatggc cacagcagct agggcgttat
    29341 agatattata gagcccgccg atttggatac cgtattcttg gccgtctatg acaaagcgag
    29401 agcgattgtt ggtcaactca accagttttg tcaaacgata gtcgagatca ggacgtttgc
    29461 atccacaacc ttcacagata taggcaccca agtttgcata ggtattatgc tcatatttga
    29521 ggatgccttg gcagtcagga cagagaatcc cttcggtatt gtagtgagcc agttgggctg
    29581 gtcctttttc caagtcaaaa ccaaaatact ctatagggtt tggaatagtt ggcttgtaga
    29641 aaagtggact gtctccgtta aggagaacag tagcagttgg aactttccga atggcttcca
    29701 atatcatgtt ataggtagta tagatttcac cgaaacggtc catctggtca cggaagatat
    29761 tagtaatgac aaaaagacta ggctggatat agtcacagat acgagataga ctggcttcgt
    29821 caatttcgag gacggcaata tttttcccag tttttgaaga tttggctgtt aggaaggttg
    29881 ttgcaatccc tgtaatcatg ttggcaccgc ttgggttggt tagaacttga ccataaacct
    29941 cttttaaaat gccgacagtg agggcagttg tcagggtttt tccatttgtt ccagtgacaa
    30001 cgacaatctc gtagttctta gctaggtttt gtaaaatatc tttatcaaat tgaagggcga
    30061 ctttacctgg gagcgtactt ccacgtccaa gacggcttaa aacgaagtgg gaagaacgcc
    30121 cagcaagaag gcccaaagta gtttttaagt tcatgtttct attatatcac aaaagaggga
    30181 aaagacagat ttggtgtttc gtagaagtaa aaacagccca aagagggctg tcagttaaga
    30241 tgagatttca acttaaatcg caaacaagtc attcaagttc tcagccaagg ttgggtgagt
    30301 gaagatttgt tttgtgaagt aagtgtaagg aatcttgttg tccatagcaa cagtaatgat
    30361 gttgatgatt tcttgagaac cttctgagaa gatgcttgct ccaagaattt cttttgtttc
    30421 agtattgaca acagctttga aggcaccgcg aaggtctcca tttacgtgac cacgaggcat
    30481 tgctgcaacg gggatttcct taacagcgta tggaagtttc aaatcagctg cttggctttc
    30541 agtcaaacca acttgtgaaa gtgcaggtgt gatgaacata gtatttggca cattgagacg
    30601 gtcttcaagt gtgtagctgc catctccagc aaggtagctg taaacaacac ggaagtcatc
    30661 aagtgaaatg taagtaaatt gaaggccacc gttgacatct ccaactgcaa agacaccagg
    30721 aacgtttgtt tgacagtgtt tgtctacttt aatagcacca cgttcagtta gttcaatatc
    30781 tgtattttca agttgaagtg gttctacatt tggtttgcgt ccagttgcgt agagaagggc
    30841 gtcgaaacgg taagtttcgt cttcagttac gacaagcact tggtcaccat cgtttttgat
    30901 ttcagtagta tggatatttt gaagcaattc aatgccatct tcttccatgt attgtttagc
    30961 aagagctgcg atggaaggtt ctgcacgagg taggaatgta tccaaggcat ctaggactgt
    31021 gaccttgctt ccaagtttgt tgtaaaggcc ggcaaattca agaccgatat ttccgccacc
    31081 aaggattcca agtttttcag gtaatttgtc caagctttgg atacctgttg agtcaaagat
    31141 gtttttgctt gtagcaagtc cagggattgg caagacgttt gaaacagcac cagtgttgat
    31201 gacgattgtt tcagcagtca gttctttctt ttcatcacca gcttggattt cgatgacttt
    31261 atttgaaagg aagtgagctt ccgcatcaaa gatatctacg cctgtaccag caacagtcgc
    31321 atagttttta ccgttgaggc gaccagtgat cgtgttttta gtagcaatga cttcttcaaa
    31381 agacaagtcc ttttcagcag caactagcaa ggttttagtt gggatacaac caatgttgat
    31441 acaagttcca ccgtacatag ccttgctgcg ttcaacgagg gcaacttttt tgccagctga
    31501 agccaattta cctgctagtg ttttaccagc tttaccaaat ccaataacaa ttaaatcgta
    31561 tgttaacatt tatcttcctc ctattcgaat ttcattctag cacaagaaaa aaacttttgc
    31621 acaattctgc tacggaaaag acagaaaaag ataaaaacgc tttccagaca aaaaactagt
    31681 aattttttga caagcttgat aaactaaaat cctatctatt tttatataag atagaataaa
    31741 tgttcgaaaa taggaggttt tatgaaatcg aatcgttata ttattgcctt tgctggggtc
    31801 attttacact taatgctggg ttcgacttat gcttggagtg tttatcgtaa ccctattatt
    31861 gaaaaaacgg gatgggatca ggcttctgtt gccttcgcct ttagtctagc aatcttttgt
    31921 ttgggcttat cggctgcatt tatggggcgt ttggtagaaa aatttggtcc gaaagtcatg
    31981 ggaagtctat ctgcttttct atacgcaggt ggaaatatct taacaggatt tgcaatagac
    32041 cgtcaggagc tgtggttgtt gtatctcgct tatggcattt taggtgggct tggtttggga
    32101 gcaggctata ttacccctgt gtcgacgatt ataaaatggt ttcctgataa acgtggtctc
    32161 gcaacaggtt tagcgattat ggggtttggt tttgcttctt tattgactag tcccatagcg
    32221 caacacctca tcgcaggggt agggcttgta gaaacttttt atattttagg agcaagttac
    32281 tttattatca tgctcctagc ttcacaattc attaagcgtc caaatgagca agagcttgca
    32341 attttatctt cttcagggaa agaaaaaaca gcctctttga cgcaaggaat ggctgcaaat
    32401 caggctctaa aaagcaatcg gttttatatg ctttggatta ttttctttat caacatagct
    32461 tgtggtttag gcttaatttc agcggcatcg ccaatggcac aggagatggc tggcttgtct
    32521 acaagtcatg cagcagtaat ggtgggtgtt ttggggattt tcaatggatt tggtcgcttg
    32581 ctctgggcga gtttgtctga ctatatcggt cgccctctaa cctttagtat attactgctt
    32641 gtcaatcttt tcttttctct ctcactttgg ctctttacag attccgtttt atttgtagtt
    32701 gctatgtcta ttttgatgac ttgctatgga gctggttttt ctttgattcc agcttatctc
    32761 agtgatattt ttggaaccaa ggaattggcc gctctgcatg gttatatttt aacagcttgg
    32821 gcaatggctg gtttagcggg acctatttta ttagcagaga cttataaaat ggctcattcg
    32881 tacacacaaa ccttgttcgt ttttctcatt ttatacagta tcgccttggc tttgtcttat
    32941 tatctaggtc gttcaatcaa aaaagaaagt caaaaaccgc ttacatgatt ttctgtagaa
    33001 atggggctat cttttgctct ggtcttgtgt tatactagat aggttgcaaa gaaaacagta
    33061 cttttctttt gtggaaaaga agcaacacga ttttatatct agttatatga aaatacgtca
    33121 taaaaagaaa agtataaact aagccctata gggtggcttc gcaccacctt tagaaagaag
    33181 aataacgtga aatttaatga attaaacttg tctgctgatt tgctagcaga aattgaaaaa
    33241 gctggttttg tagaagctag tcctatccaa gaacaaacta ttcctttggc ccttgaaggc
    33301 aaggatgtta tcggtcaagc tcagactggt acaggaaaaa ctgcagcctt tggcttgcct
    33361 acccttgaaa aaatccgtac agaagaagcg actatccaag ccttggtcat cgctccaact
    33421 cgtgaactag ctgtccaaag tcaagaagaa ctcttccgct ttggtcgtag taagggagtc
    33481 aaagtccgtt cagtatatgg cggatcaagc attgaaaaac aaattaaggc tcttaaatct
    33541 ggtgcccata tcgtggtggg aactccaggt cgcctcttgg acttgattaa acgcaaggcc
    33601 ttgaaattac aagacattga aacccttatc cttgacgaag cggatgaaat gcttaacatg
    33661 ggcttccttg aagacatcga agccattatt tcccgtgtac ctgagaaccg tcaaactttg
    33721 cttttctcag caactatgcc agatgccatc aaacgtatcg gtgttcagtt tatgaaagcc
    33781 cctgaacatg tcaagattgc ggctaaggaa ttgacaacag aattggttga ccagtactat
    33841 atccgtgtta aggaacaaga aaaatttgac accatgactc gtctcatgga tgtggcacaa
    33901 ccagaactcg ctattgtatt tggtcgtacc aaacgccgtg tggatgaatt gactcgtggt
    33961 ttgaaaattc gtggcttccg tgcagaagga attcatggcg acctagacca aaacaaacgt
    34021 cttcgtgtcc ttcgtgactt taaaaatggc aatcttgatg ttttggttgc gacagacgtt
    34081 gcagcgcgtg gtttggatat ttcaggtgtg acccatgtct acaactacga tattccacaa
    34141 gatcctgaga gttatgttca ccgtatcggt cgtacaggtc gtgctggtaa gtcaggtcaa
    34201 tctattactt ttgttgctcc aaacgaaatg ggttaccttc aaatcattga aaacttgact
    34261 aagaaacgca tgaaaggtct caaacctgca agtgtagaag aatccttcca atcaaaaaaa
    34321 caggtagctc tcaagaaaat cgaacgtgat tttgcagatg aaaccattcg tgccaacttt
    34381 gagaaatttg gtaaggatgc tcgcaaattg gctgctgagt ttactccaga agaattggca
    34441 atgtatatct tgagtctgac agtccaagac ccagatagcc ttccagaagt ggagattgca
    34501 cgtgaaaaac cactaccgtt taaaccatca ggtaatggtt tcggtggtaa agctaaggga
    34561 ggtcgtggag gccgtcgtgg ggacgaccgt cgagagcgtg atcgccgtgg caatggtcgc
    34621 cgtggcaatg gtcgccgtga tgagttcaaa aaaggaagtc gtggcaacga tcgttttgat
    34681 aaggaaaaac gttaccgtaa ggataataaa aaaccacgca atactttaag cgaaaagcaa
    34741 acaggctttg ttattcgtaa caagggcgat aaataagaaa aagcggttca aaatgaatcg
    34801 cttttttata taaggagacc gaatgtgaaa agacaaataa aatcattata ttatctttta
    34861 taatgttatg atacaaaaaa ataggcaact tgtcaataga aaaggaacaa tttaattaaa
    34921 aatattctgg tttttaatta aattgtaagt caatgcaagt caataaggaa ttttcagata
    34981 attattgaaa aagagacgtt tttatgttat aatgatagcg attaaattcg aggtgaaaaa
    35041 tggcacattt attagaaaaa actagaaaaa ttacatcaat tttgaaacgc tcagaggagc
    35101 agttgcagga tgagcttcct tacaacgcta ttacgcgtca gttagcggat attattcatt
    35161 gcaatgcctg cattatcaat agtaagggac gtctgcttgg ctattttatg cgttataaaa
    35221 caaatacaga tcgcgtagag caattcttcc aaactaagat tttcccagat gactacgttc
    35281 aaggggctaa tatgatttac gaaacagaag caaacttgcc tgttgagcat gatatgagta
    35341 ttttccctgt tgagagtaga gatgattttc cagatggctt gacgactatt gcaccgattc
    35401 atgtatcggg gattcgcctt ggttctttga ttatttggcg taatgataaa aaattcgaag
    35461 atgaggactt ggttcttgtt gagattgcca gtaccgttgt tgggattcag cttcttaact
    35521 tccaacgtga agaagatgag aaaaatattc gtcgtcgtac tgctgtcacc atggcggtca
    35581 ataccctttc ttactccgaa ctccgtgctg tttcagcaat tttaggggaa ttaaatggaa
    35641 atgaagggaa gttgactgcg tcagtgattg cagatcgtat cggaatcact cgctctgtga
    35701 ttgtcaatgc tcttcgtaaa cttgagtctg cggggattat tgaaagtcgc tcacttggaa
    35761 tgaagggaac ctatcttaag gtcttgattt cagatatttt tgaagaagtg aagaaaagag
    35821 attactaatg acaaaggctt taatttcgat tgattataca gaagattttg ttgctgatag
    35881 tgggaaacta acagcaggtg ctccagctca ggcgatttcg gatgccatca gcaaggtaac
    35941 tcgattagct tttgaacgag gagattatat cttctttact attgatgctc atgaagaaaa
    36001 cgattgtttc catccggaaa gtaagttatt tcctcctcat aatctgattg ggacgagtgg
    36061 acggaattta tatggagatt tggggatctt ttatcaagag catggttcag acagtcgtgt
    36121 cttttggatg gataaacgcc attactcagc tttttcaggg actgacctag atattcgttt
    36181 gagagagcgt agagtgtcta ccgttatctt aacaggtgtc ttgacggata tctgtgtcct
    36241 acatacagct atagatgcct ataatctagg atatgacatc gagattgtta aaccagctgt
    36301 tgcttccatc tggcctgaaa atcatcaatt tgccctaggt catttcaaaa atacactcgg
    36361 agctaagtta gtagatgaaa atctaaatga actttttgag taacctggtt ggtgaaaatg
    36421 aaaaaatttg aaaaaaagtg ttgacaagca tcccaaaagt tgatatacta gtaaagtaat
    36481 cgacgcgggg atggcggaat tggcagacgc gcaggactaa ggatcctgtg accgctttag
    36541 gtcgtgaggg ttcaagtccc tctctccgca taggaaaaga gctagctaaa gctagctctt
    36601 ttgtatttct ctaaaaaatt agaatccagt caaatgtagt ggagaatcag cgaaaatagg
    36661 ctctttgtca actgtagtgg gttgaagaaa agctaagctt gagaaaggac aaatttcgtc
    36721 ctttcttttt tgatgttcag agcgataaaa atccgttttt tgaagttttc aaagtttcga
    36781 aaaccaaagg cattgcgttt gataagtttg atgagattat tggtcgcttc caatttggcg
    36841 tttgaatag
//
LOCUS       DAVKHZ010000011        35361 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000011 DAVKHZ010000000
VERSION     DAVKHZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.011"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..270
                     /locus_tag="VWW99_000534"
     CDS             <1..270
                     /locus_tag="VWW99_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEU9538419.1"
                     /translation="KILADSGYQGLMKICPQAQTSRKSSKLKPLTAEDKAYNHALSKE
                     RSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(322..684)
                     /locus_tag="VWW99_000535"
     CDS             complement(322..684)
                     /locus_tag="VWW99_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538420.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(685..894)
                     /locus_tag="VWW99_000536"
     CDS             complement(685..894)
                     /locus_tag="VWW99_000536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538421.1"
                     /translation="MKQKNYLVSNATVIQTYDKIAESECFLRAIGGIMLHLKLVKQEI
                     EAEKPASVEAWIISVKFKKGCYRHI"
     gene            complement(1046..2926)
                     /locus_tag="VWW99_000537"
     CDS             complement(1046..2926)
                     /locus_tag="VWW99_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9538422.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQSYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLIAIEANKACRANDIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSSINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2920..3789)
                     /locus_tag="VWW99_000538"
     CDS             complement(2920..3789)
                     /locus_tag="VWW99_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9538423.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALISYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3873..6518)
                     /locus_tag="VWW99_000539"
     CDS             complement(3873..6518)
                     /locus_tag="VWW99_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9538424.1"
                     /translation="MENIVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVATFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSMDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6609..7889)
                     /locus_tag="VWW99_000540"
     CDS             complement(6609..7889)
                     /locus_tag="VWW99_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9538425.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8060..10144
                     /locus_tag="VWW99_000541"
     CDS             8060..10144
                     /locus_tag="VWW99_000541"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9538426.1"
                     /translation="MKPLLETIDTRLGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFQPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(10184..11863)
                     /locus_tag="VWW99_000542"
     CDS             complement(10184..11863)
                     /locus_tag="VWW99_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9538427.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGE
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIHWIGNERGH
                     AGDPLWQKMNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMIIEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12619..13848
                     /gene="arcA"
                     /locus_tag="VWW99_000543"
     CDS             12619..13848
                     /gene="arcA"
                     /locus_tag="VWW99_000543"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9538428.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13906..14922
                     /gene="argF"
                     /locus_tag="VWW99_000544"
     CDS             13906..14922
                     /gene="argF"
                     /locus_tag="VWW99_000544"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9538429.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15087..16034
                     /gene="arcC"
                     /locus_tag="VWW99_000545"
     CDS             15087..16034
                     /gene="arcC"
                     /locus_tag="VWW99_000545"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9538430.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVEADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            16245..17756
                     /locus_tag="VWW99_000546"
     CDS             16245..17756
                     /locus_tag="VWW99_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9538431.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     IIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17778..19109
                     /locus_tag="VWW99_000547"
     CDS             17778..19109
                     /locus_tag="VWW99_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9538432.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICQDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            19204..19331
                     /locus_tag="VWW99_000548"
                     /pseudo
     CDS             19204..19331
                     /locus_tag="VWW99_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(19511..19804)
                     /locus_tag="VWW99_000549"
     CDS             complement(19511..19804)
                     /locus_tag="VWW99_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538433.1"
                     /translation="MGVLGYYEGFVPYVSNQYKNQAEEEDKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQIS"
     gene            complement(19792..20043)
                     /locus_tag="VWW99_000550"
     CDS             complement(19792..20043)
                     /locus_tag="VWW99_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9538434.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEY"
     gene            20247..20324
                     /locus_tag="VWW99_000551"
                     /pseudo
     CDS             20247..20324
                     /locus_tag="VWW99_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20441..21325)
                     /locus_tag="VWW99_000552"
     CDS             complement(20441..21325)
                     /locus_tag="VWW99_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9538435.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21470..22621)
                     /locus_tag="VWW99_000553"
     CDS             complement(21470..22621)
                     /locus_tag="VWW99_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9538436.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22767..24533)
                     /locus_tag="VWW99_000554"
     CDS             complement(22767..24533)
                     /locus_tag="VWW99_000554"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9538437.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATLDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24546..25274)
                     /locus_tag="VWW99_000555"
     CDS             complement(24546..25274)
                     /locus_tag="VWW99_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU9538438.1"
                     /translation="MSQDEKLIREQICDVCHRMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            complement(25289..28306)
                     /locus_tag="VWW99_000556"
     CDS             complement(25289..28306)
                     /locus_tag="VWW99_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEU9538439.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETV
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISDQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            complement(28317..30533)
                     /locus_tag="VWW99_000557"
     CDS             complement(28317..30533)
                     /locus_tag="VWW99_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9538440.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLGTEKILDLAKAAKDLEIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADKIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSRRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            complement(30555..32426)
                     /locus_tag="VWW99_000558"
     CDS             complement(30555..32426)
                     /locus_tag="VWW99_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEU9538441.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIENVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTED
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYFGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRYGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKYLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTAVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            complement(32432..33751)
                     /locus_tag="VWW99_000559"
     CDS             complement(32432..33751)
                     /locus_tag="VWW99_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9538442.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            complement(33748..34587)
                     /locus_tag="VWW99_000560"
     CDS             complement(33748..34587)
                     /locus_tag="VWW99_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9538443.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            complement(34590..>35361)
                     /locus_tag="VWW99_000561"
     CDS             complement(34590..>35361)
                     /locus_tag="VWW99_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9538444.1"
                     /translation="LNTPDFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQ
                     VPLAVSVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLF
                     NPSFKDSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWR
                     KEWYITIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSR
                     TQYARANTIGVIQILGGMLIIVCINILFRERKRLKGGK"
BASE COUNT        10454 a         7693 c         6332 g        10882 t
ORIGIN      
        1 aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga tatgtcctca agcacaaact
       61 tcacgtaaat ccagcaaact caagccacta acagctgaag ataaagccta taaccatgcg
      121 ctatctaagg agagaagcaa ggttgagaac atctttgcca aagtaaaaac gtttaaaatg
      181 ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct
      241 ggtattatca atcatgaact aggattctag ttttgcagga agtctattga aatactaagc
      301 ctacacagac tgatttcaat tttaccattc ttcatagtca aaaccatcat aatctggata
      361 ttgttctggc gacgttccaa ccgaacgtac gagataagct tcttcgtcct ctgtctcgat
      421 ttctctcagc acttggcatt caaaacgcca ttcatcaccg aagtcgaaaa taaacttaaa
      481 tttttgtttg acacttaggc tatccagata gacattttct gtgtaacgtt cttcaacatc
      541 gtcactaaca aagctaagaa agtaagaatc tgcatgactc cactcaacat tatccatgaa
      601 aaatgcgtga gcatgatcat ttgcaaaatc aaaactccat aaaatcacat ctgctagttc
      661 cgctaacgtt tttgtttttg gaatctatat atgtcggtag caaccttttt taaatttgac
      721 ggaaatgatc caagcttcta cagatgctgg cttttcagct tctatttctt gttttactaa
      781 ttttaagtgt agcataatac ctccaattgc tcttaggaaa cattcacttt ctgctatttt
      841 atcataagtt tgtataactg tagcatttga aaccagataa ttcttctgtt tcacgcgaaa
      901 caccctgcgc caagcaaggt gttcttgttt ttatactcaa tgaaaatcaa agagcaaact
      961 aggaagctag ctgcaggttg ctcaaaacac tgttttgagg ttgcagatgg aagctgacgt
     1021 ggtttgaaga gattttcgaa gaatattaag tcgtataaat cgtcgacgct gtcgcaatgg
     1081 tatgccactg gttggctgta gttgctgcga aatccttgtc tgcgtagaag tcagtaaatg
     1141 gtaggatttc aacttccagc tcgtcgatgc ggtcaagctg accagccaga taaacctcga
     1201 tacggctttc tgctcgtttg atgcgttgca agagtccgcc catacggatg tcaactgtat
     1261 ccaaaccaaa gaccttgttt tctttcagcc attggtgact aaagagggca tggaagtctt
     1321 caatttggct tctaagttct ggtaattctt gtctggcgat ttgttgtaaa ctttctttat
     1381 catccgcttg gtaggcatga cgaatgcgtc gtcccacatc tactttgcta cttaaaatag
     1441 cattcaactg ggcctgagtt tcaaagagat aagcatagtt tccagctttt tctttaatgt
     1501 tagcaagcgt ctcagcagcc tgagcgaagt gcggtttgtc ctgttcaggt gtcatgtgtt
     1561 gatcaagaat cggacaaaga atatcctgat aaaaaacata gcggttggga ttgatactgc
     1621 tgagattgcc tggtaggtct ggtaagaggt tggcaaggtc aatctgcata aaatcctcaa
     1681 ccgttagacc agtattggtc ttgaagtgcg cagacaaacc atctaggtca ttgcgatagc
     1741 tgagttctgc ccagatttgc aagcttggta ggatagagaa ctgggctgtt tccccaccgt
     1801 tatcccccca acccgtcacg atgacttctt tgatgtcatt ggcacgacag gctttattgg
     1861 cctcgatagc gatgagacgg ctgaaatggt tgtgaggtgt aaagccaatc cacttccaag
     1921 ctccccctgc aaatgcaagg tcatggctaa tcttgtgatg attgcggaaa ttacggttgt
     1981 atttttcctc gctatcctga taataatccc agtaaaccag agtcacacgg tctttgagac
     2041 ggtctaggta gacacgagtt tcctctggaa tttccacatc acggtcgtac tggccatccg
     2101 ctgacatgag tttgaagaac atatcactcc acatctggca gtggaaacca tatttgtcag
     2161 caatatccag cacgcgctcc aagtgttggc acatgaggag actacgatcc acaacaccgt
     2221 tcaaaatcag gtagcgtccc aaaccaacca agtgggcttc gtccatcccg atattgacct
     2281 tgcgagtctt cagtttagac aacgtggcaa acatgccatc aatcaggtca taaacctttt
     2341 cttcgccaat gagaagaatg tcctctacat cacggagctc ctgcacttcc ttgacacccc
     2401 atttgacaaa ggctgacagg tgggccaagg tctggatgca tggtacaaag gtcatgtcaa
     2461 actgttgggc ataggcttcg atttcctgca actcctctgc tgaataagct ccacggaaat
     2521 agccaaagta agattgccct tcaatctggt aagtgtcttc catgtaaagc tcaaaggttg
     2581 agtagcccat gagagccaag acctcaatca tctgcttggc agaagccaca ttcagcaccg
     2641 catttcgaga acagtcaacc atgtaagcca aatcttcgta agctgcttgt tcctcaatct
     2701 ctactttatc agcttctgct agaactgtta ccaacaagga taaggcacga tagagttggt
     2761 gaggtttgcg gtaggtcaat tgatagtgac cttcctcacc ctggatagag atagatgctt
     2821 ggtcagactg agtgacagcc acttccacat ctggtagaga aatatgacct tttaaaacct
     2881 caatagcttg cgtttgtttg ggactaagtc ctgtaaatct taccattgct tttcctcctg
     2941 tagccagttg acaagagcac cgtagagatt ggcatctgcg tgataggtgc aggcctggat
     3001 aactggtgcg accgtgtatt cttcgtaggc atcgacaaag tcttcaacag ccttcttgac
     3061 accttggata aaatctggat tttgactgat agagccaccc agactgatga catctggatc
     3121 gatcagatac tggatattga gcaagccttg cgccagattg cggttcatgc gctcaatggc
     3181 ttcttgacaa aggatattac cagctgcggc ctcttggtaa atcttgcgac cgtcccaatc
     3241 agtatgacca gatttttcaa tcacgtatcg taccatattc ccagttgatg ctagttgcga
     3301 ccagttatta agtttttcag caggggcaag ggttgtcatg tagccaaatt ctccacccag
     3361 accgtggcga cctcgatgaa gtctaccatt gataatcatg gctccgccaa tccctgtccc
     3421 aatcacgaca caggctgcat tttcaagctc tggatgagct agtagttcac tgagtccaac
     3481 gcagttggca tcattttcta aatggacagg tagctgataa gagataagcg cctcatacca
     3541 agaaaagcca tggatgtagg gcaccgcact gaagccatca atcacacctg tctcttgatt
     3601 gactgcacct ggaacactca tagcaatccc actgtaatcc tgttctgaca agcgttgatc
     3661 tagccacgct agtaaatcct ccaagttttc aggcgttgaa atacttgtct tatccagtat
     3721 tttcccatca ggagtcagac tggcaaactt aatcccagtc cctccgatat caatcgttgc
     3781 aatggtcatt gtttttacca taaaaagggg cgaatcagca gttagttctt actttttcgc
     3841 ccctcctttc ttttgatgtt tactttttga aattaaattt cttctttttt gatgaattct
     3901 gtacgaatct cttgtggagc caatagtcct gaatgaactg gatatggtcg ctcaagtaag
     3961 tcaagaatgg tttgttgatg ttctgatatg cgcacatttt cttgactcat attatagtaa
     4021 cggaggacat agccttcttc attttccgcc actttaaagg ctgttggaca aacttgcggt
     4081 atgctgagaa cagaatggct caagaggcta ccagtcgcaa ccacgcttcc ttcctgtcta
     4141 gcaagctgaa ggctggtaaa cggtgtctgc aaggctttgg cacgacgata ggctgagaag
     4201 cgttcttggg cttggtggca ttcaatcgca aattcgactt caaactcccg caagcattgt
     4261 gcttctggcg ttgggaagta gccccagtca cctagctcac ctgatgcacg caaaatggtc
     4321 acggcaatag tgttatcccc aaggatttcg tattcattca atcccttatt ggatacagtc
     4381 accccttttt catcgtcata cagactgaca aaagcttgtt ggtgttgagg attttcagga
     4441 ttttcccatg aagcagctgg tttgtttggt cgtgtcacca cctcatagat actttcagaa
     4501 tcattacttg gacgcgtgtt atgagtcttg accaagagac ggatacggtg atccttggca
     4561 gtgttagtaa agcgagtctt gaagcggatt tgtggattgt caacgaagac agtcaactca
     4621 gtttccagag gaatgtttgt caattcttct gaccgtccag cctcacgctt cataaactcg
     4681 atgatacctt gttgctcttc ttctagcttt tcatccgcgc tgacaggcac ggtcaattca
     4741 tgtttgagca agattttagc atagcaagct gtgttttcca agacctcgtg gcccttaagc
     4801 tctgcaaaga ttggctctgt tccttttggt tggaaataga tatactcgtt tccgatgtcc
     4861 ccacggtctt caaagcggat aaagtcttca taggcttcgt gagttgtctt atcatagact
     4921 gtgatgttgt catccatact caccgttacg aatggtgtat caatcactcc gttttggtaa
     4981 ataccctcac ggtgttcttg ttctccttcc agcaattgga aggttgtcca agaaagcggt
     5041 gctaggtgaa ctggaatggt cacgcgcact tgtcgagcaa tacgagcttg gcggaacttg
     5101 tcttttggta aatcgtattc aaaattagct ccgaggtctt cgattgtagc ctctacagga
     5161 cgaccatcca agtcctccac acggtaactt ggcaagataa gagcagccat ctttttgtag
     5221 ccttctgttg ggtgcaattc cttgaaatca caagtcgcca catcaatcac tgtgctgaca
     5281 gtatcgacct tatcatgcaa gcctgtgtta atgacagtaa agagatagtc actttgagcc
     5341 ttatccgtag caattttacc cttccactcg ttgagcaaat tacttttaac aaagtttcct
     5401 acttggttga ccttggcaaa acgcgtttcc atctcgcggt gaacttcgtc cacgctacag
     5461 ccacagatac tatcatgtgg cgcattctgc aaaagtgttt tccaagcata ggtcaactgg
     5521 tccttgtggt tgtgtccacc agtgataata gtcaagggtt ctaccacttg ctctaggagg
     5581 ttgctatttt cttggaaggc ttgttttagg taaatgcggg atgaagaagt gttggcaagt
     5641 gtgtaccagc catctgtttc ctgactggtc aactcacctg taacagttga taagtgttca
     5701 ggaagcgcac cttctacagc ttgaacatat tcatcaaagg aactatgaac aaagattaca
     5761 tccgggaaga gttcatttgc cacacgaatg gcttcgctca gatttttctg tacaggctgg
     5821 tggtcacagc cgttcatcat caaccattgg ttggtcgaag cgtaggcacg cacatctgac
     5881 aatttttgtt tccagaaggt caaggcctca tctttgtcaa ctggaatttc attcccgtta
     5941 ctgtaccagt tggcaaagag aatacctaaa acacgactac catccacacc ctgccagtac
     6001 atttctgaaa actgggatgt aaactgctca tcttcaagga cttggttgtc aaatccaatc
     6061 ggcttcacac cacgaccaaa ggtcgccacg tgaatgcctg atttttgaag aatttgaggc
     6121 gcttgtccca tatttccaaa ggtatctgga aagtagccaa tctgggttga tttaccccat
     6181 ttggcagctt cttgttgacc aatcaaggta ttgcggacat tggcttcact ggagatcaaa
     6241 taatcatcct gcaagatgta aaagggacca attttaagct tgccctcgtc aatgtagcgt
     6301 tggaccttgt cacgattttc agggcgaatt tgtaagtagt cgtcaaggac aatagtttgt
     6361 ccatccaagt ggaaactctt gaactcaggg tcattttcaa agagatcaaa gagattgtca
     6421 aacaattcca ccaactgcat acgatggctt tcaaaaggca agtaccactc acggtcccag
     6481 tgactatgtg agataatatg tacaacaata ttttccatga agtaaaacct cattctaaat
     6541 ttaaatttta acgttttaaa tgtaaacttg tgattctgat aagaatcggt tgagctaaag
     6601 cgagcccctt agcgaatatc caagtaatct aagaccaact cacagaacat catgttagcc
     6661 caagagaacc attcacgaga gtagagggtc ggatcatcta cgtggaagct ttcgtgcatg
     6721 acacctgtac caccatcgca ggcaaccagc tgatccagca agaatttttt ctctgcctta
     6781 tctcttgttg tcaatccttg gatagaaagg gcgattggcc agatatagcg atagaaggta
     6841 tgagaacttc ctagaccgct agcgtattct ccttggtaga aatatggatt ttcagagctc
     6901 aaaatggtac gacgagtagc ttgatacact tcatcatcga ccgaacagta gcccagatag
     6961 ggcgcagcta gtagacttgg tacatttgga tcatccatga tgctggcatt tcctaggcca
     7021 tccacttcaa aagcgtaaat cttttcgccc ttgctgttgg tggtgtaagc gtagtttttg
     7081 attccttctt ggatttcatc ctgaagacgc ttggcatcag caataacact ctggctatca
     7141 gctaggttta atgctgcgaa gatttcttgc acataaccca agactactac agcaaacata
     7201 tttgacggaa tcaagtaact atactgacag cagtcatcgc tcggacgaaa ggctgaccaa
     7261 gtcatacctg tcactgcaaa gtcaggtcca aagccatcat ttaccaaggt gtcttcctta
     7321 cggtccgtat ctcggacaaa acgataagga gagttcttgt ggtcttgttc caccgtccac
     7381 agatggagaa tttccttagt cgctgcgaca aaagtctcat caaactgacc agtctcgcca
     7441 gtctctttcc agaggagata agccaactgc aaaggatagc aaagcgaatc cacctcatac
     7501 ttgcgctccc agatccagcc gttaaggtct gtgtggtcag tctcgtggtg ccctttccag
     7561 ttctcctcaa tgttgaagga gttagcatag ggatccttga gtaccaaggt catctgacgt
     7621 ttgaccaaac ctgcaatggt ctgacgcagg agggcatctc ttttagctac atgaaggtag
     7681 ggtctgagtt gggctgtcga atctcgaagc cacatggcag gaatatcccc agtcaagaca
     7741 aaagttgaac catcttctaa gatttcaacc gtattgtcca aggtatcggt gtagcaacgc
     7801 tcgaaaacat ccacccactc tggatggtcc ttagcccgct ctgctacttc atctagccat
     7861 tctctaacaa tttctttcga ataaatcatc gtgcaattcc ctctttcaaa caagtttcat
     7921 gttcagtata tagcttttga gacagaaaat acatacacaa atgatagtca tttttctaca
     7981 aaatagttat ctttgaaact ccttttctaa aggccctctt tttctgatat aatgtagaaa
     8041 aacagctaaa ggaaagacta tgaaaccact acttgaaacc atcgataccc gcttgggaac
     8101 taccaacaag catgcctttt ctcggggaaa taccctgcca tacacaggcg tgccttttgg
     8161 gatgaattac tttgtgcccc agaccagtga ccaggatgga tcttggttct tcgatccgca
     8221 tctgcctatc tttcagggga ttcgattaac tcaccagccc agtccttgga ttggggacta
     8281 ctcttggctc cttctgacac ctgtcaccag tcaactgggt ggagacagcc tctttcaccg
     8341 ccaatcttcc tatgataggg ataaggcctc tttccaacct cattatctaa agattttctc
     8401 cctacgctat cagattgaaa cccagctcac accgacttgc tatggtgctt ctattcgttt
     8461 gaagcaaaag caaggcaaag ccctctccct ctatcttcac gcagcagatg aactaacagt
     8521 tgagcaggta gataagcgga ctctggctct cagacaagaa gggaaaacag agaccaataa
     8581 aaatccactg atgctcttca ctgccctgca aatgaacacg gatattcttg ctatcagcca
     8641 agaagctgga gactggcgaa ttgacttagc aagtagtcaa accgagatgc aactagccac
     8701 ttctttcatc tctccttctc aagctctaat caatctacct caagaagatt ttgatagctg
     8761 taaaacaagt gcccaagcgg attgggaaaa tctcctccat cgttttgaca ttatagagac
     8821 aggagaggct gaccgaacct tctttgacca ctgcctctac agactcttcc tcttcccaca
     8881 gactttttat gaggttgatg agtcagggca agccatccac atggatctgg ctactggtac
     8941 tgtcaagcct ggtgtcctct ttagcaacaa tggtttctgg gataccttcc gcaccacctt
     9001 ccccctcttt gcccttatca taccggagca ctatcaacgc tttttagaag gtttcctcaa
     9061 tagctaccgc gatactggtt tccttccaaa atggctggct ccagatgaac ggggtatgat
     9121 gccaggtaca cttttagacg gtattatcgc agatagcgcc tgcaaggaca tggcccctga
     9181 cctagaagga gaactcttcc aagccatgct cgaaacagcc agcaaagccg accctctcgg
     9241 aatcaatggc cgccacggac tagcccaata ccaagaacta ggttacctct ctaccgacca
     9301 ccacgaaagt gttagccata ctctagacta tgcctatagt gacttttgta tcgctagctg
     9361 tgccaaaaaa ctagagaaca tagaaatcgc tgaaacctac aaggctgctt cacaaaatta
     9421 ccgccagcta tttgacgctg agacaggtta catgcgagca cgagacaatc aaggaaactt
     9481 tcaccctgac ttctctcctt atagttgggg gcgagactac gctgaatgct ctgccattca
     9541 agctacttta ggcgttctcc acgacatccc tggcttaatc caacttatgg gtggaaaaga
     9601 aacctttagc aactatcttt tgaaagcttg tcaagatgct cccctctttg aaacaacagg
     9661 ctatggttat gaaattcacg aaatgagcga gatggctact gctccttttg gacaaatagc
     9721 catttccaac cagcctagtt tccacatccc ttatctcttc cgctacagcg attaccctga
     9781 ctacactgcc cttcttatca agacgctccg tcagaaagct tttcagccaa gttgggaagc
     9841 ctatcctgga gatgaagaca atggtagtct ctctgcttgg tacatctggt cagctctcgg
     9901 attttatcca acctgtccag gaaaaccaag ctatgacctc ggaatccctc tctttgacca
     9961 tctacgagtc tacttagcta aagaagataa atggctggat atccatacta aacaaaacca
    10021 caaccatttt aactttgtca aagaatgccg actggacaaa acactcgtat caactattca
    10081 acaccaagac ctcctaaaag ctgaacaact aactttcacc ctcagctggc taccaaatca
    10141 ctaataacca aaagaacctt tgcatcgcgc aaaggttctt cttttatgaa acttggactt
    10201 gaagttgatc cacaagctga ccatccaccg ttaggttcag atagaaatcc aaggttttat
    10261 ctcctgcgaa atacaaggtt ggtagcgtca gccttttttc agtctcacca gcttgaaacg
    10321 caaggacttg aatctcatcc tgataggcga caccatggac acctgtcccc ggttgaatcg
    10381 aaatcttagc ctctaaagag gagctacttt cactacgttc aatcctaaaa tgaagagtct
    10441 ctccctttgc cacagctagg cttttttctg caaatgctag ttcctgaaca acttcttttt
    10501 ttgataatcc aggagtttta taaagggaaa tcttggtcaa tacaggaata gattgtgcct
    10561 cgataatcat cacgcgcacc ttttgtgcct ctactagcgg acctcgcaag aggcgcttat
    10621 gaccaactgt gaagcccctg ccaaattcct gccagacacc atccacctct acttgcacat
    10681 gaaaagcagc gattcgttgc cctagcttca aatcttctct taactcaatt acatcaaaag
    10741 ttttaggaga acctaaatcg agttctaact ggatgggcaa gtctgcatcg cttgcccaag
    10801 agctggtctc aaggccgtct gtcaaatggc gacaagcaaa gtctgcggaa agagctggac
    10861 cagatacctc agctcccaga gccaaatctt ctttatagag ctcattgcga taggtcgcaa
    10921 attcataaag tcgttcaata tcctttgcat caaagagccc agcttgattc ggcggaatat
    10981 taagcaagag tggagttcct cgccctactg agtgaaagta gatttcgacc aactcctcga
    11041 gagacttagg atcctgatcc tcatggtaga accagcctgg acggatggaa acatctgcct
    11101 ctccgatcga aaaaatcgtg cccgagggat ccccgtgctg aagatagttc agctctgctt
    11161 ctgttcctag tttatcagga ttcatctttt gccacagtgg atcacctgca tgccctcgtt
    11221 cattgccaat ccagtggata ctggtgcctt ctgttgaaaa aatcaaacaa tcgccctgca
    11281 agtcacgaat ggtttcaaac catttttcaa attcataatt aaccttttgc gcgccctctc
    11341 ctctggcacc atccatccaa acctcagcga acttaccagc attcccatag ttaggatttg
    11401 ataagatttc cttcaactga gccagataat aggcattgta gtccgcttct cggtccacat
    11461 gatagagggg actatgggca tcccacggtg acaggtagac ccccatatcc atatcaaact
    11521 ctgtggcagc ttgggatact tcaaggagca agtcgccctc tcctctcctc caaggactga
    11581 ccttaaccga ataatctgtg tgagctgtcg gataaaggac aaagccatcg tggtgcttga
    11641 ccaccaaaat caactttttg aagcccgttt ccttgagcac acgaacccac tcacgcgcat
    11701 ccaactgact cgggttaaag cgctcaggat cctcctgtcc agtcccccat tcttggtcat
    11761 aaaaggtatt aggaccgaag tggataaaag ctgctagttc atctcccaga taagctagct
    11821 gagtctgact tggtaacggt ccatgcggtt tgattttctt catcgttata tatctccttc
    11881 tttagcaact tctcttgttc acaaggttat ccacaggttg tggataataa accatgatct
    11941 aacagccagc tctttccaac tccaactggt aggctcagga tttctctcct caatattccc
    12001 tcttcaaaca gctctgtcat atggttctgc taacacctct cagaatgcta ctctccataa
    12061 ttattctcag gcttctttca ggtcattgtg tgaagaccat tcactctcca gacttttcca
    12121 cagattgtga ataagtgatt attctaaact atcacctcgt cgactataac aaaaaataaa
    12181 aagagggtca ataagataat tatctcttcc taaatactca gaaaagattc tataatttgt
    12241 acaattgaac aagaaatgct tctccaaaat ctatcttcaa aagaatgttg catcttttta
    12301 tcctttcaca caaacatttc tctttataca gatccaagtg ataaccagat gataattgga
    12361 cactttttgg tcatttgtat attttttgaa aaatttttta aaaaaatttt atatcctttt
    12421 taatagaagt ttatgcattt gtatatatgt attacatcct atgttgtgta taataacata
    12481 aaattgtgct aaatatcata atttatatta ttttccgaga aattatagcc attgacatta
    12541 cctaggaatt gcgttataac atacatgtaa gcggtaccca aaatggtgcc aagtcaaaat
    12601 ttttaaggag gaaaatacat gtcttcacat ccaattcagg tcttctcaga aattgggaaa
    12661 ctgaaaaaag ttatgttaca ccgtccaggc aaggagttag aaaacttgtt gccggactat
    12721 cttgaaaggc ttctttttga tgatattcct ttcttggaag atgctcaaaa agaacatgat
    12781 gcatttgccc aagctcttcg cgatgaagga attgaggttc tctacctaga acaactcgct
    12841 gctgaatcat tgacctctcc agaaatccgc gatcaattta tcgaggaata cttagacgaa
    12901 gccaacatcc gtgatcgtca aaccaaggtt gctattcgtg aattacttca cggcatcaag
    12961 gacaaccaag aattggttga aaaaacaatg gctgggattc aaaaagttga attgccagaa
    13021 attcctgacg aagctaaaga tctaactgac ttagttgaat ccgattatcc atttgcaatt
    13081 gacccgatgc caaacctcta tttcactcgc gacccatttg caacaattgg aaacgccgta
    13141 tcgcttaacc acatgtttgc agacactcgt aaccgtgaaa cactctacgg taagtatatc
    13201 ttcaaatacc acccaatcta tggcggaaaa gtggatttgg tctacaaccg tgaagaagat
    13261 acgcgtatcg aaggtggaga cgagctagtt ctttctaaag acgtccttgc agtaggtatc
    13321 tctcaacgta cagacgcagc ttctatcgaa aaacttttgg tcaacatctt caagaaaaat
    13381 gttggcttca agaaagtttt ggcctttgaa tttgctaaca accgtaaatt catgcacttg
    13441 gatactgtct tcactatggt agactatgac aagttcacta ttcacccaga aatcgaaggc
    13501 gaccttcacg tttactcagt tacttacgaa aacgaaaaac ttaaaatcgt tgaagagaaa
    13561 ggtgacttag ctgaacttct tgctcaaaac cttggtgtag aaaaagttca tttgattcgt
    13621 tgcggtggtg gcaatatcgt agcagctgcg cgtgaacaat ggaacgacgg ttctaacact
    13681 ttgaccatcg cacctggtgt ggtagttgtt tatgaccgca ataccgtgac caataagatt
    13741 ttggaagaat acgggcttcg cttgattaag attcgcggaa gtgaattggt tcggggccgt
    13801 ggtggacctc gttgtatgtc tatgccattt gaacgtgaag aagtgtaatc gctgttcggt
    13861 attcgtcaat agaaaatgta aaaaatagaa agaggaaata ataaaatgac aaattcagta
    13921 ttccaaggac gcagcttctt agcagaaaaa gactttaccc gtgcagagtt agaatacctt
    13981 attggtcttt cagctcactt gaaagatttg aaaaaacgca atattcaaca ccactacctt
    14041 gctggcaaga atatcgctct cctatttgaa aaaacatcta ctcgtactcg tgcagccttt
    14101 acaactgcgg ctatcgacct tggtgctcac ccagaatacc tcggagcaaa tgatattcag
    14161 ttgggtaaaa aagaatctac tgaagatact gctaaagtat tgggacgtat gtttgacggg
    14221 attgaattcc gcggattcag ccaacgtatg gttgaagaat tggcagaatt ctcaggcgtt
    14281 ccagtatgga acggtctaac tgacgaatgg cacccaactc aaatgctcgc tgactacttg
    14341 actgttcaag aaaacttcgg tcgcttggaa ggcttgacat tggtatactg tggtgatgga
    14401 cgtaacaacg ttgccaacag cttgctcgta acaggtgcta tccttggtgt caatgttcac
    14461 atcttctcac caaaagaact cttcccagaa aaagaaatcg ttgaattggc agaaggattt
    14521 gctaaagaaa gtggcgcaca tgttctcatc actgaagatg ctgatgaagc agttaaagat
    14581 gcagacgttc tttacacaga cgtttgggta tcaatgggtg aagaagacaa attcgcagaa
    14641 cgtgtagctc ttcttaaacc ttaccaagtc aatatggact tagttaaaaa agcaggcaat
    14701 gaaaacttga tcttcctaca ctgcttgcca gcattccacg atactcacac tgtttatggt
    14761 aaagacgttg ctgaaaaatt tggtgtagaa gaaatggaag taacagacga agtcttccgc
    14821 agcaagtacg ctcgccactt cgatcaagca gaaaaccgta tgcacactat caaagctgtt
    14881 atggctgcta cacttggtaa cctttatatt cctaaagtat aattttagat aataaaccgt
    14941 ctaccaacag ctatgggggc tgcgactaat agctttagtc cggtcctctt ttatataatg
    15001 gtaatctatt atttcttata aaatatgtga aaaatcatta aattgaaatc taaacgcatt
    15061 ctattgagtg tgataaagga gaatttatgg caaatcgtaa aattgtagta gctttgggag
    15121 gaaatgcgat tctttcttct gacccatcag caaaagctca acaagaagct ttagttgaaa
    15181 cagctaagca tcttgtaaaa ttgattaaaa atggagatga tttgattatc actcacggta
    15241 atggacctca agttgggaat ctcttgctcc aacatttggc atcagactct gaaaagaacc
    15301 ctgccttccc actcgactca cttgtcgcta tgacagaagg tagcatcggt ttctggttga
    15361 aaaatgcttt gcaaaatgct ctcttagatg aaggcatcga aaaaaatgtt gcctctgttg
    15421 taacgcaagt tgtcgtagat aaaaatgatc cagcttttgt taacttgagt aaaccaatcg
    15481 gtcctttcta ttcagaagaa gaagcaaaag cagaagccga aaagagcgga gcgactttca
    15541 aggaagatgc tggccgtggc tggcgtaagg tcgttgcctc accaaaacct gttgacatca
    15601 aagaaattga aaccatccgt actcttttaa ataatggtca agtcgtcgta gctgcaggtg
    15661 gtggcggtat tcccgtcgtc aaagaaaaca atggacattt gactggtgtc gaagcggtta
    15721 ttgataaaga cttcgcttcc caacgtttgg cagaattggt tgaggcagac ctcttcatcg
    15781 ttttgacagg tgtagattat gtatttgtta actacaacaa gccaaaccag gaaaaattgg
    15841 aacatgtgaa tgttgcccag ctggaagaat atatcaaaca agatcagttt gcaccaggta
    15901 gcatgcttcc aaaagtagaa gcagctatcg cttttgtcaa tggtcgtcca gaaggaaaag
    15961 cagttattac ttcccttgaa aatctaggcg ccttgattga atctgaaagc ggaacaatta
    16021 ttgaaaaagg ataagttgtt ttactaataa gatgtattct atttctagta tctttatatc
    16081 aaattagaaa ttattcttga aaacatgtac aatatttcaa aagatactag ttttttagac
    16141 tttaatatgg taaaacaaat ataaatagaa agcgttttct taaatgttta tttaagaaag
    16201 tagttggttt tttacacttt gttagacatc aggaggaaaa acaaatgagt gaaaaagcta
    16261 aaaaagggtt taagatgcct tcatcttaca ccgtattatt gataatcatt gctattatgg
    16321 cagtactaac ttggtttatc cctgcggggg cctttataga aggtatttac gagactcagc
    16381 ctcaaaatcc acaagggatt tgggatgtcc tcatggcacc gattcgggct atgctaggta
    16441 ctcatccaga ggaaggttcg ctcattaaag aaacgagcgc agcgattgat gtagccttct
    16501 tcatccttat ggttggtggt ttccttggca ttgtcaacaa aactggtgct cttgacgtag
    16561 ggattgcctc tatcgtgaag aagtataagg gccgcgaaaa aatgttaatt ttggtactga
    16621 tgcctttgtt tgccctcggt ggtacaactt atggtatggg ggaagaaaca atggccttct
    16681 atccactcct tgtgccagtt atgatggccg ttggttttga tagcctgact ggtgttgcaa
    16741 ttattttgct cggttctcaa atcggctgtt tggcatctac tctgaatcca tttgcgacag
    16801 gtattgcttc agcgactgcg ggagttggta caggggacgg tatcgtactt cgtctgatct
    16861 tctgggttac cctgactgct cttagtactt ggtttgttta ccgttatgcg gataagattc
    16921 aaaaagatcc gactaagtca ctggtttata gtactcgcaa agaagatttg aaacacttta
    16981 acgtagaaga atcttcatct gtagaatcta cacttagcag caaacaaaaa tcagttctct
    17041 tcttatttgt gttgacattc atcttgatgg tattgagctt cattccatgg acagaccttg
    17101 gcgttaccat ttttgatgac tttaatgctt ggttgactgg tcttccagtt attggtaata
    17161 ttgtcggttc atctacttct gcactaggta cttggtactt cccagaaggc gcaatgctct
    17221 ttgcctttat gggtatcctg attggtatta tttatggtct taaagaagat aagattatct
    17281 cttccttcat gaatggtgct gctgacttgc tcagtgttgc cttgatcgta gcgattgctc
    17341 gtggtattca agttatcatg aacgacggta tgattaccga tacaatcctc aactggggta
    17401 aagaaggctt gagcggtcta tcttcacaag tctttatcgt tgtaacttat atcttctatc
    17461 tacctatgtc attcttgatc ccatcttcat ctggtcttgc cagcgcaact atgggtatca
    17521 tggctccact tggagaattt gtaaatgtcc gtcctagctt gattatcact gcttaccaat
    17581 ctgcttcagg tgtcttgaac ttgattgcac caacatctgg tattgtgatg ggagctcttg
    17641 cacttggacg tatcaacatt ggtacttggt ggaaattcat gggcaaactc gtagtcgcta
    17701 ttattgtagt gaccatcgcc cttcttctcc ttggaacctt ccttccattc ctataaaata
    17761 gtgagtgagg tgatttcatg aaaatagata taacaaatca agttaaagat gaatttctta
    17821 tatcattaaa aaccttgatt tcctatcctt cagtactcaa tgaaggagaa aatggaacac
    17881 cttttggaca agcaatccaa gatgtcctag aaaaaacttt agagatttgt caagacatag
    17941 gtttcactac ctatcttgac cctaaaggtt attacggata tgcagaaatc ggtcagggag
    18001 cagagcttct ggccattctc tgtcatttgg atgttgttcc atcaggtgat gaagcagatt
    18061 ggcagacacc gccatttgaa gcaactatca aagacggctg ggtattcgga cgtggtgtcc
    18121 aagatgataa aggcccttcg ctcgcagctc tctatgcagt aaaaagcttg ctggaccaag
    18181 gtattcagtt caaaaagcgc gtacgcttta tctttggtac cgatgaggaa accctctggc
    18241 gctgcatggc acgctacaat accatcgaag aacaggccag catgggcttt gcacctgact
    18301 catcttttcc tctgacctat gctgaaaaag ggcttctaca ggtcaaactt catggccctg
    18361 gatcggatca actagagctt gaagtaggag gcgcctttaa cgttgtacca gacaaggcca
    18421 actaccaagg tcccctctat gaacaggttt gtaacggtct caaagaagct ggttatgatt
    18481 accaatccac tgaacaaacc gtaacggttc tcggagtgcc aaagcatgct aaggatgcta
    18541 gtcaaggtat caatgctgtc atccgactag ctaccattct tgctcctctc caagaacacc
    18601 ctgctctcag ttttcttgca acacaagcag gtcaagacgg cacaggaaga caaatctttg
    18661 gtgatatagc agatgaacct tctggtcacc tatcctttaa tgtcgcaggt ctcatgatca
    18721 atcatgaacg ttctgaaatc cgtattgaca ttcggactcc tgtcttagct gataaggaag
    18781 aactagtaga gttgcttaca agatgtgcac aaaactacca actccgctac gaagagtttg
    18841 actatctagc gcctctatac gtcgcaaaag acagtaaact cgttagcaca ctgatgcaaa
    18901 tctaccaaga aaagactggc gataacagtc ctgctatttc atccggtggt gccacttttg
    18961 ctcgcaccat gccaaattgt gtagccttcg gcgccttatt cccaggagcg aagcagacag
    19021 aacatcaggc aaatgaatgt gccgttctag aagatttgta ccgtgctatg gatatttatg
    19081 ccgaagccgt ctatcgactt gcaacttaat caggcaactg tttctaccaa aaaaaatcga
    19141 ccgattaatg aactgcaccc caaaagttag acagaataaa tctaactttt ggggtgtttt
    19201 attatgaaat tgagttatga agataaagtt cagatctatg aactaagaaa gcaaggacaa
    19261 agcttcaaac agctttcaaa aagatttgtg tggatgtttc tggtctaaag tcatctgaat
    19321 ctttgagatg agctttataa atcgattttt tcagtttttg cactggtgtt tcgataaact
    19381 caaacttttt agccgtggta ttgcctgatt ttatagtata ttgaaactag aatagtacac
    19441 ctctacttct aaaacatttt tagaaatcga tttgactgtc ctgaacgatt tgtcctgttc
    19501 ttatttcatt ttacgatatt tgagccactt cgtctttaac ggctttattc ataagctctt
    19561 gtaatttttc tttactatca attacttctg attttccgtt gtaatttatt gtaataggtt
    19621 ttaacttacc taatttctcg acacgctcat taatttgatc ttttttgaag gctgcatatg
    19681 tttttcctaa gattttttcg aaaatatatt tatcagatag cggtttgtct tcttcttcag
    19741 cttggttttt gtattgattt gaaacgtaag gaacaaatcc ttcatagtaa cctaatactc
    19801 ccataagttc aaaagcttgt tttctaattc aaaccattgc aactcagatt tcagcttttc
    19861 agataaatcc tgctcatcca aataatgact tgaaattagt gctgaactcg tttctgtatc
    19921 ctgtacaggc tgagcaccca taccagcaaa aaataaactc gttcctagca agaccgaaca
    19981 agctcctatt gcatatttcc tcaaagaaaa acgctgcttt ctctcaaatt gaaattcttt
    20041 catcccatct cccatcattc attattactg tatattttgt atatcagaaa tagtttgtat
    20101 tcacaaatct ttctagttat tcccttatca ttcctaatta agggagataa catacaataa
    20161 tttttagtta aatgtatatc gatgtttttt gtttttctta ataaacgcaa tacaaaaaga
    20221 gcctgttgcc aagctctttg tactcaatga aaatcaaaga gcaaactagg aaactagcca
    20281 caggttgctc aaaacaccgt tttgaggtag tagataagac tgacgaagtc agctcaaaac
    20341 actgttttga ggttgcagat agaactgacg aagtcagctc aaatatacgg caaagcgacg
    20401 ctgacgtggt ttgaagagat tttcgaagag tattagtcta ttatttcttc tcagcgcgaa
    20461 gggctgacaa gatttgtgtt cggatatcat ccacaccatt tggagtattt ggtaaaaaga
    20521 tagtttgatt tcctttagag gcaaaggtat tcaaggtatc caaatactgg ttggtcaaga
    20581 ggatagacat gatttgttct tctgtcatgc caacattggc ttccttgagt tcggtgatag
    20641 actctgccaa tccatccaca atcgccttac gttgttgggc aatccccaca ccatgaaggc
    20701 ggtctttttc tgcttctgct tcagctgcag tgacaatttt aatcttgtca gcttccgcca
    20761 attcttgtgc tgcgacccgc ttacgttgcg ccgcattgat ttcattcatg gattgcttaa
    20821 cttctgcatc tggttcgacc ttggtaatca aggttttcac gataatgtag ccgtaagtgg
    20881 tcatttcttc tgctacttgg tgttgaacct caagggcaat ctcatctttt ttctcaaaca
    20941 attcatccaa ggttaatttt ggaacagaag aacgaagagc atcttcgata taagatttaa
    21001 tctgagattc tggacgtatg agtttatagt aagcatctgt cacgctctgc tcgttgacac
    21061 ggtactgagt cgctacattc atcataacga acacattgtc cttggtctta gtctcaacca
    21121 caatatcact ttgcaacaag cgcaactgaa tccgtgctgc aatcgagtca atcccaaaag
    21181 gcaagcgaat atgaataccg ctattggcaa ccttttggta tttcccaaag cgttcaataa
    21241 tcgccaccga ctgctgacga accacataaa ctgtactcag tgtgactatc accaatagga
    21301 gcacacaaac aatcagaaaa atcatgaaaa atattgccat aatggaacct ccacaagtat
    21361 ttttctagta ttatagcaca tttaaagaag gctgtgccgt ttttactgcg atttttcctg
    21421 aaatgtcaat aattagaggt gaattgtcct attgtcgtcc aatctcttgc taaaataact
    21481 ctttataaaa ggcaatcgtt tcttctaagg ttggcataaa tggatttcct ggtgcgcagg
    21541 catcaatcaa ggcattctta gaaaggtatt caaagtcgaa atctttttct tcaataccaa
    21601 gttcagtcag tttcttagga atacctactg tctcagaaag cttctcaatc tcaacaatcg
    21661 cataatcggc acattcttga tctgatttac cttctacatg aagtcccaag gctttggcaa
    21721 cattgcggaa agcttctggt acacgtttag cattttcacg ttctataact ggtagcaaca
    21781 tggcacagca aacaccatgt ggcaaattat atactgcacc gagttggtga gccattgagt
    21841 gaacataacc aagaccagca ttattaaagc tcataccacc aaggaagatg gcattgacca
    21901 taccttcacg cgcttcaata tcatgtccat tttctacagc acgaggaaga tattccttga
    21961 taagctcaat agcaccaata gacagtttct tggttacatc ataagcacca ggtgttacca
    22021 aagcttcaac agcgtgagtc agagcatcca taccagtagc agcagtcagg cctttaggtt
    22081 tggaaagcat gagttccggg tcattaacag agataagagc aaggctattc ttgtcaacca
    22141 ttaccatctt aaccttgcgt tcttcatcag taatcacata gttaatggta atttctgctg
    22201 atgtacctgc cgttgtattg atagccacaa ctggcaagcc ttttttagca gacttatgaa
    22261 gcccttcata atcctgtggt tttccaccat ttgtagccat gatagaaata caactagctg
    22321 catcctgtgg agaacccccg ccaagactga tgataaagtc acagccatgt tcttgcaaag
    22381 cagctacccc atctgtgaca tttttacaag tagggtttgg ctctacatcg ctaaagatga
    22441 catattcgat tccttctgta tccagtggtt ttaaagtctt aggtaaaata tcactgcctt
    22501 cgatgtactt atctgtcacc aaaagtgcct ttttatagcc aagttccttg atataaggcc
    22561 ccacttcatt tacaacacct tttccaataa ggttgactgc cggaacataa aatatagcca
    22621 tataattttc ctcctgcgcc tccgcgctat ctatttaaca tcattatatt actttttgta
    22681 ttcgttttca attatttgtg aattgtttaa caaatctttt taaaaggata ggagggacgt
    22741 aagttcacct cctatataaa caagttttat ttatgtagtg gtcccaacaa ctgacatgca
    22801 cgatagtctg ctgattctag atcttctgtt ccaaataagg accaattttt aggtctaaag
    22861 atatcttcct caggtacatt atgcatattg acaggaattc tcaacataga agccaaggta
    22921 atcaagtctg ctccaatgtg tccatatgtt atggctccgt gattagctcc ccaattattc
    22981 atgacgtcat agacagactt gaaagcacct tttcctgtca aacgtggagc aaaccaagta
    23041 gttggccatc ctggatctgt acgattatct aaagtatggt gaacatcttc aggaagttca
    23101 agtgtgtaac cttctgcaat ttgtagcact ggaccaaccc cttttagaag attgagacgt
    23161 accattgtta ctggcatatc ccccttcgtc aagaaacgag ttgagaatcc tcctccacgg
    23221 aagtattcgc ggtttgctgg tgggaagtct gtattttcaa gcatagcctg cacttcactt
    23281 tcttccaact cccagaatgg tttcataaca ggtttgccat ctagagtagc ttgacctgta
    23341 ccatccaatg tacaagatcc agagttgatt agatgtaaga agccagctgc agcacgaccc
    23401 tctaaagtat gtcccgttac acgtttaaca gcctctgggc tccaataagt acgcacatca
    23461 gcaaagattt gtggagtatt tgttaataga taattaaaga gcatagacac accatttagt
    23521 gaatcattct ctgtcgcaaa tacaaatggt tttcgaatac cattccagtc aaactgagta
    23581 ttgaggaaag tttccataaa atctccattt ggaaaatggt ctgtccactg acgttgacct
    23641 tggaaaccag ctactaaagc atggtgacca actgcttctt cttcaaaacc aagttcagca
    23701 agtcttgggt taccaaccat taagtcacgt ccaatcatga acatcttaat aacaaattcc
    23761 cattgttttt ctttctcttc tttagaaaga atcaaatcct cacgatttcg gtcaactcct
    23821 tctttgatat gttcttttac ccaaaccata gcacgttcaa actcttcagg gtcgtaaata
    23881 ccacggtcca tacggcgtgt gaactccgtc atatctaccg attcatttcg cattcctaag
    23941 tattcttgga agaaatccgg atttacaata gaaccaccaa tccccatcga aacactaccc
    24001 attgataggt aagcagtgtc tctcatcaag ccagttgcaa gagctgcacg cgcataacgt
    24061 aaaagttttt ctttgacatc ttctggaata tctgtatcat tagcttcctg aacatctcta
    24121 ccataaatcc caaaggctgg aatccctttt tgagcatgtg aagctagtac agctgcaaga
    24181 tagacagctc ctgggcgttc tgtcccatta aatccccaaa tagcatgagg aatatctgga
    24241 gacatatcca tagtttcact accataacac cagcatggtg taactgtaat tgttgcgcaa
    24301 acatttgatt ttttaaacaa ctcatgggaa gctgcagcct ctggaacacg accaatggta
    24361 gatggagaaa tcacacattc cacaggttcc ccatctggat atttcaatgt gcttgaaatc
    24421 aaatctgcca cacttttagc catgttcatt gtttgtactt caagtgattc gcgtacacct
    24481 tgacgacgac catcaatagt cggacgaata ccaatacgtg gatgttgaat catactattt
    24541 tcctcttatt tttctgtttc tttcatacta ccatcgccat tatatttacg gtagcctggg
    24601 tgacgacctg taaccttata attttctcgc attgagaata gacgttctaa agtcggacga
    24661 gcaatttctt gctcctcaat gccctttgta gaaagtaaca ttcttccgtg gaaggttgtc
    24721 tttgcgacta attctaaagt ttccatacgg tagtatgctg taatgacatc gcttccgaca
    24781 gtcagagctc catgattttc taatagcatg acatcatgat cgggcagata aggtgtaatt
    24841 gcttctggca cttccattgt agacggaact ccaaatggcg ttataggaat tgcccctacc
    24901 acaatcgcgc tctcaattag tgaataagta tctaaaggaa tgtgtgcaag agcaaatcct
    24961 gttgcaatcg gtggatgcgc gtgaacaact gaacgaacat cctcacgttc ttcgtagcac
    25021 cgaatgtgca ttttaatttc actagaagga cagtaatcac cttctgcttc taaaatctct
    25081 cctttaagat ttaacttcac cagcttttct ggtgtaataa aacttttgct gataccagta
    25141 ggtgttgcaa gaatggtatc ctcatctaat cgaacagata cattcccatc gttagcagca
    25201 acccaaccaa gttgccacat cctatgacaa acatcacaaa tctgttcacg aattaatttt
    25261 tcatcttgag acataaactc actcctttct aaaaatgaag ttcgaattca agatttccgt
    25321 tactgttaat ggtaatagta accatatgat ttttctgatc aaaattgaga gatggctgat
    25381 cataggaatt atttgttcct gataatgaat gaatagtagg aagtttatct atacctctta
    25441 atactatctt tgtaaccctt ttctctccaa actgagtatc tttgatataa tgttcttcaa
    25501 tccacttaat cgcacctttc ttggtcaatt gtggtagttt ctttgcctga tttgcatctt
    25561 gagcattaga ccatagtgaa tctttatttg ttctaaaatt atttaaattc actttcaatc
    25621 cattacttat tctctcaaaa ataccatagg tatgaggttc aaattcaaca tccatttcta
    25681 aagaattaaa ttttaatttc ccagtctgtt taacattctc atttaccttg taattataga
    25741 caaaccatct attatctaat ttctgagcaa agatatctcc ttcataattc ataggataca
    25801 gcttatttaa gtattctttt ctagcctgag tagagctaaa ttctggagaa gtgatttcct
    25861 taatctgaat acggctacca gaaacagact ctttcaattt atctgttttt aaaacacctg
    25921 gaatagctgg aataacatta taacgaccag tcatgtataa tggagtttgt tctctatcag
    25981 tatttacgtt tacaaagaac ttaccatttc ctttatttga atagtctcca tgtatgaata
    26041 cttttgtatc ctcaagtacc ttttctttgg atggtgctgg atgcgctatt acatatctaa
    26101 aaaattcttt tattacttcg ctaaagagta agctttcttt attatttaca ccataggtat
    26161 aagctgggtg ttcaaaatta taaactacac ctccattaag gtaaacccct aaagcttctt
    26221 ctcctagcat agactctggt tcagtcaacc attgtctatc tccttgactt tttccgatat
    26281 taccacttgc aaacagtttc catttacctg tctcatacca cttccaagta tccatcaaac
    26341 caccccattg ataagtatga tttgaaagcc aaagtccctt catataactt tctgtagttg
    26401 aatcatttcc ctcagcagca ggtgtatttt tgaacatgaa aataaggttc ttccagtact
    26461 tatcaacaga tttctgaaat gctatcttac cgttttttcc aaatgctttt tctattgcag
    26521 aaccattatt ttgttcagcc catataaaat atcctccatt tttagctgat acttttaagt
    26581 aatctgccgc cttattttca atatcatttg cccaaatcca ataattctct gtactgaaaa
    26641 taccatgcaa atttggatat ttttgataca ttttatcaat ccagctggta cttaaccaat
    26701 gtgccgaagt ataataaggc atattaccag cagtgtaaac cgtcagaata actggaattg
    26761 gttcattttt tccagtctta ggatttacat atttcgctgc ttcttctaac atatgttcat
    26821 aaaaatctct agcagctcca tctcttttag gaagatcatc tggatgtaac tgtataacag
    26881 tatatggttt taagtcatct ggtatattct cccacttacc tgttaatgta tctcctcccc
    26941 aaaaagtata tttcccttgg ttaaactctt ctccgttagc atataaaggc atcataagta
    27001 acggatgttc attattaatt tcacgacgtt tatcaacttc tgaccaatca ttaggattct
    27061 taaatttacc atttgccata aacaaggctc cacctaaaac caattcgtca gcccatgtat
    27121 gtttccctga atatgattta agttctattg tctgtgtatc tttgggaatt tcaacatcaa
    27181 ccagaaatgc cgatgtatca tacttaattc cttttggata ttttgaatct gatgagtaaa
    27241 tgactttacc atccgcaaca acttcaaatt tttgaatatc tgcgtaatct gaatttaaat
    27301 gatttgcact cctatcaatt ccgacatagg aaataaatct tgtaacatta gcacccgaaa
    27361 catcatattt tatactagac gggttacttg ctacagttcc caatccctta ttaaattcga
    27421 cttccttacc gtcaattagt aattttattt tattattaga cccgttatta ccaggtgtaa
    27481 acggtttatc tttctgaact gtttttgatt tatcgtcatc accatgagta gcatcaaccc
    27541 attctaaatc agaaagatat actcctccat taattccttt attaagaaga tctggagtta
    27601 tagtttgagt actaacagtt ttaataagtt tagcatcagc aaatacaact tcatcacccc
    27661 atgtatgttc tcctgcgaat gattttaagg atattttttt agcgttttgt ggaattgtca
    27721 cactaataaa ttgtgcctgg gtattatatc tgaatccttc tggattagtt acactactag
    27781 aatagacaac ctttccatca atttctattt ctattctatc cactactgca tggtctgatc
    27841 tagaactatt agcactttga tcaattccta tataagtttc aaatttttca aacccttgat
    27901 cgctaatatc atagctgatt actgatggag aatctgcgac tgttcctatt cctttatcaa
    27961 aatatttaac tttatcatca gaagtgagca atgaaatctt agtatgctct cctttatttc
    28021 ctgttgtaaa tggagcatct ttttgtactg tttttgtttt atctatatca ccatgagttg
    28081 cagaagacca atccatatct gaaagatatg tcatctcttt atcaatttct ttatctttag
    28141 taataagttt ttctaagtta ttccctgtta ctctaacagt ttcagtacta gctctgttat
    28201 tagaattact ttgaactaca tctgcataaa ccaaattact tcctaaaaaa cttgttaaaa
    28261 cagccgtagc tattaaaatc tgttttgcta tttttttact ttccattttt atttcctcaa
    28321 tatttacata caacttcctc aataacaaat aattttgtaa gatattctgg aacacaaagt
    28381 gctaaattac ttaaactgac ttttattcca gcattcatta attcatctcc ataaaagaac
    28441 tcatcaaatc cagagacgga atagcattta tctttatcta aaccactaaa ataaactctt
    28501 gtataacctg gattcaactt actattcaat tgagcataca ataaaatcga ttggcattta
    28561 tcttttgaaa caacttgcca actagcagaa tcacaactaa aaggactgtt tatcctatag
    28621 aaatccccat attgaaacaa tgaacgatat tttttataaa actgaatttg ttctctcact
    28681 tgttcttttt cttctaccga caatctcgta acatctaatt cataaccgaa agaactaaaa
    28741 taagctacat ttgtcctagt gtccaaactc gtttgtcttc tactttgttc atttggagaa
    28801 atagaaacat gggctgtcat cattgatgga gaatatccat aagaagttcc atgctgaatt
    28861 ttcaatcttt ctatcgggtc cgtatcatca ctcgtccacg cttgcggtgc ataatacata
    28921 agtcctaaat caaaacgtcc accacctgaa gaacaagatt caaatagaac tgaagggaac
    28981 ttagttatta aacgatctaa tagctgataa agacctaaga tatatcgatg accaaattcc
    29041 atctgctgtt cagaatcaag ttcaatacta taaatatctg ttatatgacg attcatatcc
    29101 catttgatat aatccagatt tgttttgatt atgattttag aaatagaatc aaaaaggtaa
    29161 tcaacaactt cctgtctcgt caaatccaac acatattgat ttcttcctac tgacttctct
    29221 ctgtctaaca aatggatagt ccaatcggca tgattcttgt acaaatcact atcaatagaa
    29281 atcatttcag gctcaaacca caaaccaaat tgtaaaccta ttttgtgaat tttatctgca
    29341 agaaatccaa taccttcagg aaggcgactt ctatctgtta cccaatcccc cagagaactt
    29401 ttgtcatctt tcctatgacc aaaccaacca tcatccagta caaataattc tatctctaaa
    29461 tctttagcag cctttgctaa atctaaaatt ttttctgtac ccaagtcaaa ataaaatgtt
    29521 tcccaactat tgagtattat aggacgacta gcttgagaaa aattaggatt aactaaatga
    29581 tgtcgataaa aatctgatag ttgttggctc atctgattca tcccttgatc agaatagatc
    29641 acaattgctt ctggtgtcac aaaactttcg gcaggattta ataaaaaacg aaattctact
    29701 ggatttattc ctaccgttac tctagcggtg tcaaaatggt caacttcaat catattttga
    29761 aaatttccag aatagataaa gttaaaacca taaacctcac cactatgctc tgtagtttcc
    29821 ctccttgata gcataaagaa aggattttgc tgaggacttg atgagtatct caagctatct
    29881 attgcttgaa tacctggtct aagcgaagtt ctatataact gacgttccct cagccatgct
    29941 ccagaaaatt gaataaagtc atattgacta tcaggtagat ctagctgtaa gctcattgct
    30001 ttcaaaagag tcaacttatt attgctatta tttaaaaatt ccgtatttct aactaagaca
    30061 ttgtattccg gaaaaattgt gtaatttagt ctaacagtta aatctaatat ggaatctgtt
    30121 aaaataatcg ttaatgtttc cgcacactga ccaatatcat caaatgttga aggtagagag
    30181 ttaagacgat ttttcccttt ttcaagttca aaaccttgat attttagtgt cgttatccta
    30241 ctaccattca ttccttgaac ttgaaaggct ggatgccgaa aatctccttt cccatatgaa
    30301 gaatattcat tttgtatcat agactgttga atatcctcaa attcttcaga aaaagatact
    30361 tcaaatcctc ttttttcata ggccatcaat tgattataat tttccttgac atgaatcctt
    30421 ttcccaaaat gtagatgttg aagttctcca ttttctaaaa ttctaaaaat ataactacta
    30481 tctttcattg ccaaatgaaa aacggtctcg tccttattaa tataaatcgt catctctacc
    30541 tcacaatttt ttctttactt cttcagtatc acgatagagt acggtgccaa ttctatctca
    30601 tcttcttgaa tagtaaaatc tccaattact ctggtaaaat tctctatcga aagattctct
    30661 atttttattt tttgtttaat ctgagcagga tttctcagag agagaatttc agtagaagtc
    30721 ttctgattgt gacacttata accataaatt tcaaattttt caggacttcc tcctattttt
    30781 ttactatatt ttagtgtttg ataattctcc tctatccatt tgacggcacg agcattggct
    30841 ttccagcgtt cttcatcaaa catatcaaaa gaatagtgaa actcccaaaa agcattccct
    30901 cttgttgaaa taaataatag ataagcttca aaatctggta ttgatgcaaa catttgatga
    30961 tccatgtacc aggtatttgc actgacagca tagattggtt catgattata aagcgaccac
    31021 ataggtaact gtatctcacg tttttccaaa aattcttgat actgaatatc tcggtaagta
    31081 atcatacgat tgatatcatt acctgcattc tctgtaaagc ctacatcttg agatatttga
    31141 atccataaac tattgaccca ctgtaaaaac catggactag gatttacata agaagtcaag
    31201 tttaaccaac aatcttttcc tcctctctcc tttcttagat ctatcaacag ttgaattaag
    31261 aactcataaa ccgctgtcat ggtatacata ccgtgcggtc cactcttatc aggtttgtca
    31321 ggttgaagta accaaccatc aattttccaa tagctgatat cgaattcttt ttgatattcc
    31381 aacatctttt tcttcatttg attgagatag ttaaaatcag ccacgtttac atcatttgaa
    31441 atcaaatttt tagatccaat atttaactct ggatgtgctt ctaaccaatc actcataatg
    31501 atttctgtcc cattataacc acctcgggga ccaatccaca atcctaggct tgaaccaaat
    31561 ccatttacaa gatatttaat atttctcaaa ccatttggga atttatgatt gaattcccaa
    31621 actgattgat agtttgtcca accatcatca acaacataag catctaaatg aactccatga
    31681 ttttcaaatc catatcgaat ctcagaaaaa ctttttaaaa taccttcctc tgtaatatcg
    31741 gtcatatggt cataccagga attatactgt ttacggaaat aactaggttg agctattcct
    31801 tcaacatatt caaaaaatgc ctcttgaatc tcttcttttt tataagaaca tgctccccca
    31861 ataatacaag accacaaact cttttcttga tttacaacag ttccaaagta atatcttgat
    31921 acataatgtc taccatctac cttgttttca gacattggaa attccattcc taagaataaa
    31981 gaattcgcat aaacaggttg tcccaattct acataataac cggaaaaatt cgccatttcc
    32041 ttaatacaat cttgtctttt cggatagaaa atattgtctt ctgtttcaaa ctcaaaactc
    32101 tctacctcta cagatttaat atcctcagta gatgattcta aaactaattg cttcgaaaga
    32161 acatcatccc tcgattcaaa gattaaacgt aaacgaatgg aaggacaaac aaaagaaacc
    32221 tgaattcgcg tatctccctc ttccctaacg attgctgaaa aatgatttga agaaagtctt
    32281 ataccattct ccaatgtaat ggtaaattct tctttcgaaa cattttctat cattactcct
    32341 gcccgtttgt ttacgatatc agtagtataa aatcgaccat ctccccaaaa gaaattacgt
    32401 cttacatttt tattttcaat tttcatataa actactctct caactcaatt ttgattacgc
    32461 tatcaatcaa gtctggtaat ggataggtaa aatgtggaac ttctccaaac tgtgcaaaac
    32521 aaattccttt gtaggcattg gtcgtccagc tttctgaaat tttcacctca cttccatcat
    32581 gaagaaagct cattcttttt acgttttctt tactaatacc aagaagagct aaaggaccta
    32641 taggttgttc aaatacatga gcataaaccg tatttccatt acgagtatag tatccccaat
    32701 ctggttttgg taaattttca tctccactac atccataaat tgcctcacca tttcgagaca
    32761 tccatcgatg aaaattatct aatattttct tacttgaatc attaattctt cccaaagcat
    32821 ctgggcctac attaagaatc atatttccgt ttttactaac acattccact aactttctta
    32881 tcaaagtttg actagactta tatagataat ctgtaggatt ataagcccaa ttgttattca
    32941 tagttagaca aagttcccaa ggaacaggtt cacctttaaa gtttcgaata ccttcgtgag
    33001 gaacaatttg ctctggacta acaaaatctc cagcgtaaga ggtaatttca tctgtaacga
    33061 tacttccgaa tccttcacct gatgtttcta atctattatc tacgataacg tttggctgat
    33121 aatgacgaac catgtctatt aattttgaag ctccccattt ttcacccacc atgtcttcat
    33181 aagaataatc aaaccataat atgtcaatct gaccataacc agttacaatt tctttaactt
    33241 gattgtgcaa atactccaaa taactgtcaa agttaatttt ctcatctcga taggcttcat
    33301 ttcctctcat tggatgattt agatcagcat atttagggaa atcaggatgg tgccagtcaa
    33361 tcaaactaaa atataagcct acttttaaac cttcagctcg aacagcatct acaaattctt
    33421 taactaaatc tctacctgca ggtgtatttg ttgctttata atctgtaaat tttgaatcaa
    33481 acaaacaaaa cccatcatga tgtttagccg ttaaaaccat atacttcatc cctgcagctt
    33541 ttgcttgttt agcccattct ctagggttat attccttagg atcaaaagct cgaaaatatg
    33601 gttcataatc ttcaatagat aatttctgat gactacgaat ccattcccct tttcctggaa
    33661 tagaatataa cccccaatga atgaacatcc caaatcttgc ttctctaaac cactgagttc
    33721 tactattgat agtttcaagt gtatgtttca tccttttact gctcctcctc ctaaagcttc
    33781 aatgaagtat ttacttgtaa atgcgaatag aatgattggt ggtaatacga ttacaacaat
    33841 agctgaaaat agaccattat aattagtatt aaaacttgta gtaaattgac ctagtaatgc
    33901 tggtactgtt aattcatttt tatcaatcac caacatagat gcccaataat attcattcca
    33961 gttattaata aaggcaagta ctgctgctgt taaaattcct ggtctagcaa ttggcaacat
    34021 cactttccaa aaagtttgag catatgtcgc accatcaatc tctgctgcct cctccaattc
    34081 ttttggaata atttcaaaat agttctttat aataaagaaa ctcatagcaa ttccactaca
    34141 agagtagacc agaattagag caactggggt attataaatc cccaactcat ttactaatct
    34201 ataaactgga aaagtcatag ctgttgctgg aataaataga gtagaataca gcatagaagt
    34261 caccaacttc ttccctctaa attccatcct agcaataaca tatgaagaca tggagatcat
    34321 aacaatgcta ataataactg atatagacac tactttaaag ctattccaaa agtaagtgaa
    34381 aacatgaagt tttgtaaaaa catttatata accttcaaga gtaaagtcag tcggtaaact
    34441 aatacctgga tttttcatag gatctgcttt taatgatgat aaaaaaatcc acgcaatagg
    34501 gaaaatagaa attaaaatgg taaggagcat ataaccatat ataagaactt tgcctttttt
    34561 actaatacca tttatatgtg tattcataat catttcccac ctttcagtct ttttctttct
    34621 ctgaataaaa tattaataca aacgataatc aacattccac ctaagatttg tataactcca
    34681 attgtatttg cacgagcata ctgagttcgt gagctagcca ctgctaattc tcgaataaca
    34741 aaactgatac tttttgttcc tgcagcacca tttgtcaaaa agaatacttc gttatagagc
    34801 aaaaatcctg aagttgctgc cataattgaa acagttttaa ttgtcccctt aatcatcgga
    34861 atcgtaatat accactcttt cctccaacca gtaatattgt ctaaaatagc agcttcttga
    34921 acttcttctg gaatagcaaa aatttgccca agaatcataa tggttgatgc tcctgcaaag
    34981 aagatataag cgccagtcat agctactata tttaatcctg gagtcaacag tactgaatct
    35041 ttaaacgatg gattaaatag atgaataatt tggttaacaa caccatagtt tggattatag
    35101 atttgaagaa aaatcaagcc catagcagca ctagaaatga tacttggtac aatataaaga
    35161 ttcctagata tcttccagcc ttttaatttc ttagataatg taattgcaac agatacagct
    35221 aaaggaactt gaataataac accaattaca gcccaacgaa cagaattaat tagagcttct
    35281 gtaaaaaatg ggtattcttt aaaaacatac ctataatttt caaataattt atcaaatcca
    35341 agaaaatcag gggtgtttaa a
//
LOCUS       DAVKHZ010000012        32549 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000012 DAVKHZ010000000
VERSION     DAVKHZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.012"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(217..1779)
                     /gene="guaA"
                     /locus_tag="VWW99_000562"
     CDS             complement(217..1779)
                     /gene="guaA"
                     /locus_tag="VWW99_000562"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9538445.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1921..2619
                     /locus_tag="VWW99_000563"
     CDS             1921..2619
                     /locus_tag="VWW99_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9538446.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLENGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2662..3558)
                     /locus_tag="VWW99_000564"
     CDS             complement(2662..3558)
                     /locus_tag="VWW99_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9538447.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGALQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3567..4502)
                     /locus_tag="VWW99_000565"
     CDS             complement(3567..4502)
                     /locus_tag="VWW99_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9538448.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQTFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4499..5224)
                     /locus_tag="VWW99_000566"
     CDS             complement(4499..5224)
                     /locus_tag="VWW99_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9538449.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5308..5943)
                     /locus_tag="VWW99_000567"
     CDS             complement(5308..5943)
                     /locus_tag="VWW99_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9538450.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYEALSKALKDYLY"
     gene            complement(5945..6772)
                     /locus_tag="VWW99_000568"
     CDS             complement(5945..6772)
                     /locus_tag="VWW99_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9538451.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6794..7129)
                     /locus_tag="VWW99_000569"
     CDS             complement(6794..7129)
                     /locus_tag="VWW99_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEU9538452.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7130..7846)
                     /locus_tag="VWW99_000570"
     CDS             complement(7130..7846)
                     /locus_tag="VWW99_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU9538453.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWFKQADLAIG
                     DFEETINKDHYLAGYPLFNAPAEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNGIKVALLAYSYDFNGIEQYISQEDYNRYLSD
                     LNEDKMKVEIERAEKEADITIIMLQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            8359..8970
                     /locus_tag="VWW99_000571"
     CDS             8359..8970
                     /locus_tag="VWW99_000571"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9538454.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9015..9743
                     /gene="rlmB"
                     /locus_tag="VWW99_000572"
     CDS             9015..9743
                     /gene="rlmB"
                     /locus_tag="VWW99_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9538455.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9782..10693)
                     /gene="trxB"
                     /locus_tag="VWW99_000573"
     CDS             complement(9782..10693)
                     /gene="trxB"
                     /locus_tag="VWW99_000573"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9538456.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10759..10983)
                     /locus_tag="VWW99_000574"
     CDS             complement(10759..10983)
                     /locus_tag="VWW99_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9538457.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11061..11804)
                     /locus_tag="VWW99_000575"
     CDS             complement(11061..11804)
                     /locus_tag="VWW99_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9538458.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMPFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11804..12604)
                     /locus_tag="VWW99_000576"
     CDS             complement(11804..12604)
                     /locus_tag="VWW99_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9538459.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12762..13118)
                     /locus_tag="VWW99_000577"
     CDS             complement(12762..13118)
                     /locus_tag="VWW99_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9538460.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13112..13642)
                     /locus_tag="VWW99_000578"
     CDS             complement(13112..13642)
                     /locus_tag="VWW99_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9538461.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(13642..14136)
                     /locus_tag="VWW99_000579"
     CDS             complement(13642..14136)
                     /locus_tag="VWW99_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9538462.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSSEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14271..14717
                     /locus_tag="VWW99_000580"
     CDS             14271..14717
                     /locus_tag="VWW99_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9538463.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14714..15361
                     /locus_tag="VWW99_000581"
     CDS             14714..15361
                     /locus_tag="VWW99_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9538464.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15382..15963)
                     /gene="pdxT"
                     /locus_tag="VWW99_000582"
     CDS             complement(15382..15963)
                     /gene="pdxT"
                     /locus_tag="VWW99_000582"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9538465.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15964..16839)
                     /gene="pdxS"
                     /locus_tag="VWW99_000583"
     CDS             complement(15964..16839)
                     /gene="pdxS"
                     /locus_tag="VWW99_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9538466.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16991..18370)
                     /locus_tag="VWW99_000584"
     CDS             complement(16991..18370)
                     /locus_tag="VWW99_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9538467.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLSLGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18665..19588
                     /locus_tag="VWW99_000585"
     CDS             18665..19588
                     /locus_tag="VWW99_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9538468.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIKLDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19648..20253
                     /locus_tag="VWW99_000586"
     CDS             19648..20253
                     /locus_tag="VWW99_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9538469.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20272..21516
                     /locus_tag="VWW99_000587"
     CDS             20272..21516
                     /locus_tag="VWW99_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9538470.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGDHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21739..21996)
                     /locus_tag="VWW99_000588"
     CDS             complement(21739..21996)
                     /locus_tag="VWW99_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9538471.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22038..24074)
                     /locus_tag="VWW99_000589"
     CDS             complement(22038..24074)
                     /locus_tag="VWW99_000589"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9538472.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFRGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24286..25203)
                     /gene="glyQ"
                     /locus_tag="VWW99_000590"
     CDS             complement(24286..25203)
                     /gene="glyQ"
                     /locus_tag="VWW99_000590"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9538473.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQDCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25574..26334)
                     /locus_tag="VWW99_000591"
                     /pseudo
     CDS             complement(25574..26334)
                     /locus_tag="VWW99_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(26607..27449)
                     /locus_tag="VWW99_000592"
     CDS             complement(26607..27449)
                     /locus_tag="VWW99_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9538474.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27563..28954)
                     /locus_tag="VWW99_000593"
     CDS             complement(27563..28954)
                     /locus_tag="VWW99_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9538475.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29189..29566)
                     /locus_tag="VWW99_000594"
                     /pseudo
     CDS             complement(29189..29566)
                     /locus_tag="VWW99_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29781..30758
                     /locus_tag="VWW99_000595"
     CDS             29781..30758
                     /locus_tag="VWW99_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9538476.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30755..31837
                     /locus_tag="VWW99_000596"
     CDS             30755..31837
                     /locus_tag="VWW99_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9538477.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31901..32452)
                     /locus_tag="VWW99_000597"
                     /pseudo
     CDS             complement(31901..32452)
                     /locus_tag="VWW99_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9635 a         7307 c         5857 g         9750 t
ORIGIN      
        1 atattagaaa gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca
       61 gcttgaaaac tacggtctaa ttttttgtcc aaatgtaaac cacttggaac tattattcaa
      121 tttattcttt ttagcaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta
      181 accgttgatt ttacaaagct tttaattttt ttgcgattat tcccactcaa ctgttgcagg
      241 tggtttactt gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg
      301 tactgagatt ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc
      361 gatagaagtg atagcacgga ttgcaatcgt gtagtcatac gtacgaccgt cacccataac
      421 accgactgag cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc
      481 agctttagcg atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc
      541 ttcagtgatt tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca
      601 tacgatgtgg tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag
      661 agtattgagt ggttcaatca gttcaaactg catatcttct ggaagaccac ccacgttgtg
      721 gtgtgacttg atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt
      781 accttgagca aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac
      841 aaactcgtta ccgatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc
      901 aaggaaacgt ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat
      961 gtccataact tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat
     1021 caattgatcg ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc
     1081 tgatagacca agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat
     1141 atcaatgaaa ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt
     1201 acgaaggata tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta
     1261 aatgtgttta tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg
     1321 aacaaagtca gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc
     1381 atcaggtgtt gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc
     1441 acgatttcca gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg
     1501 cataccataa cagattccca aaattggaat tcctagttcg aagatttctg ggtcaatgtc
     1561 aaatgaacca tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt
     1621 gacttcacga acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat
     1681 ctcacggata cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat
     1741 tttttctaca tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa
     1801 atatcttttc tcaatattct atcacaaata agggcgaatt accaactaaa caaggcgaag
     1861 tttgactttt tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat
     1921 atgctacctg cttatatgaa aatccatgat cagataaaaa aggatattga cgagcaccgt
     1981 tgggctattg gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt
     2041 cgcatgaccc tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt
     2101 gtaggaagcg gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc
     2161 agttttactg aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac
     2221 aaaaaaacca ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat
     2281 attatccgaa tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct
     2341 tctattcctg aaaaattcat taaggacttt aaaaaagaag aaatcaccag tcatttcttc
     2401 caaaccttgc aaaaacatgg ctatcgtatc ggcaaatctc aacagaccat ctatgctcgc
     2461 cttgctaaag aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga
     2521 ttgacccagg tttcctacct agaaaatggt actgcttttg aatacgtaaa aagtcaatat
     2581 gtaggcgaac gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct
     2641 gtcacggaca aagagtcttt tttatttttc taagaataaa tctttcaaag aaatcaaagt
     2701 tacagcaaat aatatcattg ccataccaag aaaatcaatg ggataaaatt gttcattcat
     2761 tattaagaag gcaaagaaaa ccgccgatat tggctcaatt gaagccaaca agcttgactt
     2821 gaccggtcct atcagactgg ctcctttaag gaaagctgta taggcaaaaa cagtcccgat
     2881 aaggataatg cctgcaaacg caaggagaaa atcaagacta gttgggatat cggcctgtag
     2941 agcccctgta aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgaccaa
     3001 gctgctcccc cactttttaa tcaaggctat gggtaaaatg atatacagag cataagtcaa
     3061 ggcagaaaag agaccccaga acaggccagc aggtgtcatg gataactggt ccaactgccc
     3121 atgtgttgcg atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag
     3181 tgtcggtgcc accctatcct tgatacagct ataaattaaa attccgacag gacaaacata
     3241 ctgaagcacc gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg
     3301 gttgagaaaa agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt
     3361 taaaaagacc agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc
     3421 agcgattaaa agacgcaagt tggtcaagac cagagccgaa attccgtgtg ccattaggta
     3481 ttggccactc gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc
     3541 ttttaaacta tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga
     3601 ttgcggtcta atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa
     3661 atagtggctt cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg
     3721 tctagccaat ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa
     3781 aaattttgat ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaaggtttgt
     3841 aaatccttta tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc
     3901 ttgggatcat gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact
     3961 ggcccaaact tagtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca
     4021 tccaaatctt gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc
     4081 gaataaagaa aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca
     4141 ggggctgtta aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag
     4201 agctggcgaa tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta
     4261 tcaatatcta attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc
     4321 ttgctgacac tagctaggtc ataaactagt cctgcctcag tctgcaagcc atgctctggg
     4381 gcactctggc ctaaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct
     4441 ccgggataaa ttcctgcctc gatttgttct tctatttttt taataatctt ggtccacttc
     4501 atacttcttc aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag
     4561 acttaggaat aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt
     4621 cattgaccaa gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat
     4681 cctgcccctg agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac
     4741 tttcgatatc agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg
     4801 atgccaaatc tgtttgaaat tcaggctttt ctcctacttc tactagactt gctctgtcat
     4861 cttccgcatg aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat
     4921 tttgaccttt atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta
     4981 agggattttc caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag
     5041 tacgcatact gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg
     5101 acagaaaggc cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt
     5161 catttaattt tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat
     5221 tcataaattc ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta
     5281 tgctcaaatt taacattatt tagctattta gtaaagatag tctttcaagg cttttgacaa
     5341 agcctcataa ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg
     5401 gcctgcttgg tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga
     5461 tgcaagctct tgataggctt gattccaatt ctgaattttt tcattcgagc ggatatagac
     5521 tgcctgctgg tacttctctc cctcattgac aggcaaaatg gaaagcaatt taatctctgt
     5581 caatggataa tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt
     5641 cacaggaaca tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc
     5701 tccaccatat agatgagcat caaggttctc tagtaacagt cctgtctgat agccacgaat
     5761 tcctcgattg acaatcgtct ttgaagtccc aaatagctcc tgtagaggat aatactcgac
     5821 aatggaatcc ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttagatgacg
     5881 atatttggtt tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc
     5941 cacttcattc tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctgt
     6001 tggctggtgc tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat
     6061 aggcaattcc agccatttca agcatttcaa catcattttc actatcacca aaagccatga
     6121 tttcttggga tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa
     6181 tcccagcttg gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat
     6241 caaagagagc attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc
     6301 tcatcttgag cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt
     6361 ggtagaattt ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac
     6421 cttccttgac aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac
     6481 ccttgaaatg agccaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca
     6541 attcattgtt ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca
     6601 agggactcag tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa
     6661 tgcccctttg atccaactga tctaaaatct tttccaatcg tgggagatca agctgacctc
     6721 tagcatccag caaggtccca tccatatctg tcgcaattac cttaatcatc atttactttc
     6781 ctctggattc aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg
     6841 tatcaattcg ttccttagtc gcttcatcta actggtcacg atgactgcca tcctctataa
     6901 aatcttccaa aatataagtt tggtaatgat acaagggata tccttctggt gaaaaggttc
     6961 cctttggtgt tcgattgacc caagtaggat gagctttagc tgttccgata gttgtttttc
     7021 catccttctt cttgatggtg acatccatga gaacaccacg ttcagtccac ttagcattct
     7081 cttcatctcc catagattca atccgttgat tggaaatgaa gttccccatt taatagataa
     7141 tgagtttctt atctccatct ttttcaaccg tttcagatgg ttcaacaacg tgaggatgcc
     7201 ctccaaagat aatatccgct ccccaatcga tcatcttgtg ataaagagct ttttgttctt
     7261 cagttggttc caatcgatac tcaacaccca tctgaagcat gataatggtg atatctgctt
     7321 ccttctctgc ccgttcaatt tcaaccttca tcttatcttc gtttaaatct gaaagataac
     7381 gattatagtc ttcctgagaa atatactgct caattccatt gaaatcatag gaataggcta
     7441 acaatgcaac cttgatacca ttcacttcct taatgaccag cggagcctga tcacgtggtt
     7501 cgtgcgtata aactccgatt ggagtgattc cagctttctc aataatatcg gccgttgaaa
     7561 taactccctc aatttgcgaa tccaaaatat gattatgagc taaatccagc acatgataac
     7621 ctgcctcctt aatagcatcc ataacttcag caggagcatt aaagagagga taacccgcta
     7681 aataatgatc cttattaatg gtttcttcaa aatcaccaat agctaaatct gcttgcttga
     7741 accaaggagt cacatactca aaattttcat gaaagtcata ggtaccgtct tcttttttag
     7801 ctgaaaagaa aagtccatcg tggtagagca aatccccgtg ggccataatt ctggcagttt
     7861 tttcctcttc ctactcctga gacttttgct tagtcccctc ttgagaaata gtatctcgtt
     7921 tttgactagt caagggattc cttggaagct ttcaaacaac aagaccaagc ccattgataa
     7981 atcaactgct agcaagagta tcgccacaaa tcccttattg ctccacttgc gataactcct
     8041 aaaaagttta ccaagccctt cataaaacga aaagctaaac caccctgatt tcgattttgt
     8101 cttctttgta tcttcattct ccctactttc ttatgcaagc cttttctttt tattatatca
     8161 cagataagta tttctttcac aattgaattg aacttcccat ctattttcta taaatcctaa
     8221 atgccataat gctttcaatt cttgtcattt tgtgatatca tgtagaggaa atgaactaat
     8281 ccacagtggc ttattccaag tataccactt gggctttggc agtagctaac tgcactaaac
     8341 ataatataag gagagaaaat gtctgcaata gaacgtatta caaaagctgc tcacttaatt
     8401 gatatgaacg atattatccg tgaagggaat cctactctac gcgcgattgc tgaggaagtc
     8461 actttccccc tatctgacca ggaaatcatc ctaggcgaaa agatgatgca attccttaaa
     8521 cattcccaag accctgtcat ggctgaaaaa atgggactcc gcggtggtgt tggactggct
     8581 gctccccagt tagatatctc aaaacgcatt atcgctgttt tggtacctaa tattgttgaa
     8641 gaaggcgaaa ctccacagga agcctacgat ttggaagcca ttatgtacaa tccaaaaatc
     8701 gtctctcact ctgttcaaga tgctgctctt ggcgaaggag aaggttgcct gtctgttgac
     8761 cgtaacgtgc ctggctatgt tgttcgccat gcccgcgtta ctgttgacta ctttgacaaa
     8821 gatggagaaa aacaccgtat caaactcaaa ggctacaact ccattgttgt tcagcatgaa
     8881 attgaccaca ttaacggtat catgttttac gatcgcatca atgaaaaaga cccatttgca
     8941 gttaaagatg gtttactgat tcttgaataa agaaaatccc gttgcaagac ggggttttgt
     9001 gttataatag aggcatgaaa acaaatgata ttgtctatgg cgtccacgcc gttaccgaag
     9061 ccctccttgc aaatacagga aacaaactct acctccaaga agatctccga ggtaagaatg
     9121 ttgagaaagt caaggaacta gctacagaaa agaaggtgtc catttcttgg acatcaaaaa
     9181 aatctctctc tgagattact gaaggtgctg ttcatcaagg ttttgttcta cgagtgtctg
     9241 aatttgccta tagcgagcta gattacatcc ttgcaaaaac acgccaagaa gaaaatccac
     9301 ttctattgat tctagatggt ctaaccgatc cccataatct gggttctatc ttgcgaacag
     9361 ccgatgcgac caatgtttca ggtgtcatca ttcccaagca ccgtgctgtc ggagtaactc
     9421 ctgtcgttgc caaaacagcc acaggtgcta ttgaacacgt tccaattgcc cgagtgacca
     9481 acctcagtca aaccttagat aaactcaagg atgaaggttt ctggaccttt ggaacggata
     9541 tgaacggtac tccttgccac aagtggaata caaaagggaa aatcgccctc atcattggaa
     9601 atgaaggaaa aggtatctct agcaacatca aaaaacaagt cgatgagatg attaccattc
     9661 cgatgaatgg acatgttcaa agccttaatg ccagtgttgc tgcggccatt ctcatgtacg
     9721 aagttttccg aaatagacta taaaaaagtt tccagtcatc tgattggaaa cttttttatg
     9781 attaactatg ttctgtaatg aatttatagg cttcttgacc agcgatagct ccatctccaa
     9841 ccgctgttgt tacttggcga aggtctttca agcgaacatc tccaactgca aagataccgt
     9901 cgactgcagt tttcatgtgg ttatctgtca caatccatcc tgcctgatct tggatattca
     9961 attctttaac aaaatcgcta agagggtcca aaccaacata gataaagaca ccaccgaagg
    10021 cttgttctgt cacttgacct gttttcacat tttcaaatac gactgattct actcggtttt
    10081 cacccttgat ttcccttact acagaatccc agataaagct gattttttca ttcgcaaagg
    10141 cgcgatcttg taaaaccttt tgggcacgaa gttggtcacg acggtgaaca atggtaacag
    10201 tcttagcaaa acgagtcaag aagagggctt cttcaacagc tgaatctcca ccaccaacta
    10261 ccaataaatc ttggtcacgg aagaaagcac catcacacac agcacagtaa gaaacaccac
    10321 gactgttcag ttcttcttct ccaggcactc ccaaaggacg gtgtttagaa ccagttgcta
    10381 cgataactgt acgtgtttca tatgtttggt catcagtcat cactttctta aaatcaccat
    10441 ggtcttcgac attttcaaca taaccataaa tgtgctcaac accaagattt tcaagtggtt
    10501 caaacatctt ttcagccaat tcaggtccac taatattagc gtatcctggg taattttcga
    10561 tatcagatgt attattcatc tgaccacctg gcagaccacc ttcaatcaaa gctactttta
    10621 gattgcttcg agcagcatac aaggccgcag tcatccctgc aggtccagca ccgataataa
    10681 tagtatcgta catatagatt ccttctttct tgttgtaact atctttattc taactctttc
    10741 ttgtcaatca cgcaaggatt atgccttgaa aacaagaatt aaactcataa ccgcaaagga
    10801 taatactggg acaaccaaga ctccaatcat aatcatatca tagagatggg cttggcgagc
    10861 cctggctgtc ttatcaactc ccgacatggc tctcagacca atccaaatcc ctaaaaaaat
    10921 cagaacaagg atggtggtca agatcaaact ctcgaaatat aaagaaaata gttgcagtag
    10981 catgatttct ctcatttcta tcttttttaa agagtaaact cagctagtcc aactaactga
    11041 gttttccttt atctattata tcaaatataa gtccgtttgt aactagcgaa gaattctttt
    11101 gtccgctctt ctttaggggt gtggataatc tcatccggag ttccagactc gatgattttc
    11161 cccttatcta agaagagaat tttatccgca acttgggcta caaagggcat gtcatgactg
    11221 accaaaatca tggtctgacc tgacttagca gcatctgcaa tagacttttc tacttcaccg
    11281 accaattctg ggtcaagggc tgaagttggt tcgtctaaga gcaaaacatc tggtttcata
    11341 gcaagcgcac gcgctagggc aacccgttgc ttctgtccac ctgataaatg gcgaggataa
    11401 tggttttcac ggtccgaaag cccaacctta gccaactctt ccttggcaat cttagtcgct
    11461 tcttggtcag ataatttctt gacaacaacc aagccttctt tcacattatc aagtgctgtt
    11521 cggcgttcaa ataaattaaa ctgttggaaa accatagaca acttacggcg tagggcaagg
    11581 atttcttctt gagtgatttt agaaaaatca actgaaaaac catcaatctg aatagagcca
    11641 ctgtcaggtg tttcaagata attgagactg cgaagaaagg ttgattttcc agctcctgaa
    11701 gaaccaatca aggctacaac ttcccctttt tgaatatcca agttcagatg atccaagaca
    11761 gtctgtcctg aaaaggattt gcttaaattc gaaatcttaa tcattaacga aggtctcctt
    11821 tcacatctgt ttgcactgta tcaggtgcag aaatagccat ttttctctcg atgaaacgac
    11881 cgaggctttc aattccgata ttgactaccc aataaacaag ggcaacggag atgaagcgtt
    11941 caaaatagcg ataatcagct ccacctagaa tctgagcttg ggcaaagact tccacaacac
    12001 ccgcactaaa agctagagat gttcccttgg tcaaaccgat gagggaatta atcaaggttg
    12061 gagtagctac caccgctgca ttaggaataa tcactcgtcg ataaacttgc gctcgggtca
    12121 tacccagact gcgtgccgcc tcaatctcac caggattaac tgagagaatg gctgcacgaa
    12181 tggtttcact agcataagct gcctcattaa aggcaaaagc gacaatcgca aaagctgcag
    12241 ctggaatcgc attgatattg agaccagttc cccattgctg attgagggct ttcaaagcca
    12301 aagggattcc gtagtaggtc aacatgagtt gcaccaaaat cggtgtccct tttaagaaac
    12361 taacaaagaa ggcctgcaag ggatataaaa tcttgacacg attgatcttc acaatggcaa
    12421 aaagaagcgc caaaaccaag ccaaaaaggg caccaccaat tgtcaacata attgttgttg
    12481 gaagttgttg gacaattcta gggattccat caaagaccga acgtaggcta aacagcttgc
    12541 catccggaat caattgcatc aagttttggt accaatctga tgctaaaatc gttgtaacat
    12601 tcataaaaac atcctcttct gataatgatt cattctacca tgcttctgcc gtcaatgaaa
    12661 ttatttgcta cggtcagata cagactttgt ctacctacta gtttatcata ctttgaaaca
    12721 gggaagttat atattttatc tatcaaagag atagataaaa actatttcag tcccagttct
    12781 tcataagatt ttcgataacc gatttgctta acggttccat tttctactaa aatgggccgt
    12841 tttaacaaca taccgtcact tgctagcaac tcagccgctt cttggtttga caaacttcct
    12901 accttatctt ttagccctaa ttcacggtat ttgataccac tggtgttgaa aaattgcttc
    12961 aattcaaatc ctgaggtttc tagccaattc aaaatgactt cttggctagg tgtttcttcc
    13021 acgatatgga cggctttata gtccacacct aattgattta attcttgttt tgctttttta
    13081 caagttgaac atttgggata ttcgataaat tctaacatgt gcttctcctt ctattgtttc
    13141 tatctttaaa atctactcct tcatgctcca agagccaagc tttcttttcc actcctgcag
    13201 cataacctgt cagacgcttg cctgctccca acacacgatg acaaggtact aggatagacc
    13261 aaggattgcg tcccactgct ccaccaattg cttgagcaga agccacttgc aggtcttgag
    13321 caatttgtcc ataggtcact gtctgaccat aaggaatgcc ctgtaaatag tcccaaactc
    13381 tcttttcaaa attcgttcca attggcgcca agagcaagtt ggataaatcc tgaggcttgc
    13441 ctttaaagta atcatctaag caagcaataa ctgggtctaa aataggatga ctaacaactt
    13501 cttctatcgt ttcatctcct agtcccctct caaaatgctt ctgctcctga acccaaattc
    13561 catacaaata atggtcatca gctacaagtg atagaatacc aattggtgag gagtagagga
    13621 tttttacata cttcttttgc attatttctt taatttttga taggccaagc gttcgccatc
    13681 tactggcacc ttaccaactt gtttaaaacc aagtttttca aaaatatgtt gcataacctt
    13741 gttttcagca tgcgtatctg agcgaaaatc aagataatca aaaccttcaa tcaagccctc
    13801 taagaaggtt tgagcaactc cttttccctg cacatctgct gccacagcaa tacggtgaaa
    13861 gactagatac tctgactctc cagcttgcca gtttccctca taaatagctt cataggcctc
    13921 ctctgaactc ttggtcacag cagcataggc tagcagttct ccctcttcca aggctacgta
    13981 ggcttgccct gagatgatat catcgataat aacatctgca tttggatagc cattttgcca
    14041 ctggtcacta ccagcatctg ctaaacattt tttggcatcc tccatcacct gcatgatggc
    14101 atctacttca tttggaaaag ctaaacgaat ttccatcttt tttcctcatt tctgactagt
    14161 ttctcccatc atagcataat ttaagtattt ctacaagcag ttaaaacttg actttgtgtt
    14221 tttgttattt tataatctag ttatcaaaac tatataaaaa ggagttgaaa atgaaaacta
    14281 ccttttccta cccaaaatgg gcagaaattc caaacattga cctctatctg gaccaggttt
    14341 tgctctatgt caatcaggtc tgcgccccta tctctcctaa taaagacaag ggcctaacag
    14401 catctatggt caacaattat gtgaaaaatg gttacctgac aaagcctgac aaaaaaaaat
    14461 accaacgcca acagattgcc cgtttgattg ctatcacaac cctcaagtct gtattttcta
    14521 ttcaagaaat agcccagaca cttaatactc tacaaactca agcaagttca gaccaactct
    14581 acgatgcttt tgtggactac atgaaccaag gaattgaccc agctaaccct attatccaaa
    14641 ccagctgcca aaccgttaaa ctctatcatc aaactctaga cttaatcgat catactcaag
    14701 aggaggtaat ccaatgaaca ctagtcttaa actcagcaaa caactcagtt ttggagagga
    14761 gattgctaat agcgtgaccc atgctgtggg tgcagtcatc atgcttatct tgctgcctat
    14821 ttcatccatc tatagttatg aagcacacgg atttttatcc tctatcggcg tttccatttt
    14881 cgtcatcagt ctctttctca tgttcctatc atccaccatt tatcactcta tggcctatgg
    14941 ttcgacccac aaatatgttt tgcgaatcat tgaccattct atgatttacg ttgccattgc
    15001 cggctcatac acgcccgttg tcttgacctt gatgaataac tggtttggct atctgattat
    15061 tgtcatccaa tggggaacga ccatctttgg tattctctat aaaatctttg ctaaaaaggt
    15121 caatgagaaa tttagccttg ctctttacct gattatgggc tggttggttc tggctatcat
    15181 tcctgccatt atcagtcaaa cgacacccgt tttctggagt ctcatggtaa ctggcggact
    15241 ctgttataca gttggagctg gattttatgc caagaaaaaa ccttatttcc acatgatttg
    15301 gcatctcttt atcctagctg cgtccgcact tcaatacatc gctattgttt attacatgta
    15361 aaaaagttga gaaattcaat ctcaactttt ttctttacac atattgataa agtactggtg
    15421 caagcgcaca tcatcagtca attctggatg aaaagaactt accaacatat ttttttcttg
    15481 ggctgcaaca atttgattgt tcactgttgc tagaatttct acaccctcac caacactact
    15541 gataatcgga ccacggataa aggtcattgg aatcttgcca actcccttac attctgcttc
    15601 cgtgtagaaa cttcctaatt ggcgcccata agcattacgc tcgaccacca tatccatagt
    15661 tcctagatga ctctctttct gagaagtgat ttccttagcc agcaaaatta agcccgcaca
    15721 ggtcccaaac actggtaagc cagatagaat ggcttctcgg atgggaagta gcatgttctg
    15781 gtcacgtaag agcttgccca tggttgtaga ctcaccacca ggcaaaatca aacccgacaa
    15841 gtcactctga tcttgctgaa aatcatctag atttctgagt tctacactct cgacacctaa
    15901 ttgatctagc acttttgcat gttctgcaaa ggccccttgc aaggccaata ttccgatttt
    15961 catctatttt cctcgttcag ccatgagaat ttggatttca ttttcattaa taccaaccat
    16021 ggcttctcct aaatcttcag agatttgagc taggatttga ggattacgga agttagtcac
    16081 agccttaaca atggcactcg ctcgtttaac aggatctcct gacttgaaaa tacctgaacc
    16141 gacaaagacc ccctctgccc ctaattgcat cattaacgca gcatctgctg gcgttgcaac
    16201 acctccagca gcgaaattta caactggcaa ttttccatgt tcatgaacat attggaccaa
    16261 ttctacaggg acttgcaaat ccttggcagc aacataaagc tcgtcctcac gtaagttttg
    16321 aatgcggcga atttcctgat tcatcatacg catatgacga acagcttgga cgatatcccc
    16381 tgtccctggt tctcctttgg tacgaatcat ggaagcacct tcagcgatac gacgcaaggc
    16441 ttcacccaaa tccttagcac cacagacaaa aggaacttgg aattctttct tatccacatg
    16501 gaaacggtca tcagctggag atagaacttc actctcgtcg atataatcaa tttcaatagc
    16561 ctctaaaatc tgagcttcaa caaaatgccc gattctgacc ttagccatta ctggaatact
    16621 aaccgcttct tggatttcct taatcatctt tgggtcgctc atgcgggaaa ctcctccagc
    16681 tgcacgaata tcagccggaa ttcgttccaa ggccatcaca gctgccgcac cagcagcttc
    16741 tgcgatacga gcctgttcag gattctgcac atccataata acaccaccct tgagcatctg
    16801 tgccaaattt ttatttagtt cataacgatt ttcagtcatt tgttttatcc tcatcatttg
    16861 tattggtagc taccttttca ttttaagtca gaacagaatg aagcacaaga taaaaaaact
    16921 ataattgtat ggggacaaat tagctaaaaa ctatctgacc ttaacttaag gccagatagc
    16981 tcattcattt ttatttttca gccgtaaggg cagccattgt gatgtagttg tatggtttgt
    17041 tgaagtgtgg caagaagaag aggtctgtca atgccaattt atcaattgtc acatgctctt
    17101 ggatagcaag tgagaacatg tggattccca tgctaattgc aatatcatgt gaaaccattt
    17161 gggcaccaag aatttcacgg ctatctttgt caaagacaat cttaattgct acttcatggt
    17221 tgtcatgttt catgaattct ggtttttgaa gatcgttaaa gcctgtttca gttgcgttgt
    17281 aaccagcagc tttcgctttt tcaagagtca aaccagttga aaccatgtga agaccgtaga
    17341 ttgagatacc atttgaacct tgaacaccga ttccttccaa ttcatgtcca caagcgttgt
    17401 aggcaccaac gataccagtg cgaacagcat ttgaagcaag agcgatatag cttgtatctt
    17461 tacgagcatt gtcataaaca gtcgcacagt caccaacagc gtaaacgcct gggatagatg
    17521 tttcttgttt cttgtctaca aggaaggcac cgttgcggaa gagttcgatc ttaccatctg
    17581 caagggctgt gtttggacgg aaaccaactg caaggataac catatccacg tcaaagcttt
    17641 ctttgtcagt aatcaagcgt tcaactttac cgtcaccttc gattgcttta acagtttgac
    17701 ctagagacaa gcggatgttg tgatcttcca agttcttcgc catcatttgt gtgaagtctt
    17761 tgtcatagta accgttcaag acagtatcaa cgatatcaac aaggacaact tcttttccaa
    17821 gacgttcaaa ggcctcagca agttcaacac cgatgtaacc accaccaaca acggcgatac
    17881 ggtcgaggtg ttggctcttg tcagaaagtt tattgataac ttcttcagca ttttggtaca
    17941 atttcacgaa ttgtacgttt tcaagagttg ctttaaattc gcggtttcct ttaacaattt
    18001 caacaccttc gattggtggc aagattggtg tagaacctgt agcgaaaatc aatttttcgt
    18061 atgattcttt gtgctctttt ccttcaactt ccgctgtaac tactttgtta tcatagtcga
    18121 ttgaaagaac aggtgagttc atgtaaactt tagcaccttt agcttccaat ttttctttat
    18181 cagaatagaa caagccttca gcaccgtcaa tttgttcacc aatccaaaga gccattccac
    18241 atcctaggaa agagatgtta gagttttggt caaatacaac aatttcgttc tcatttccaa
    18301 aattatccaa catggtattg atacatgctg taccagcgtg gttagcaccg actacaacga
    18361 ttttactcat aaaaaatttc ctgccttcat tctaaattta ccttactagt ataccattta
    18421 ccgttagcgt ttacaagggg ggtactatca tttcagcatt ttttatcaaa aataattccc
    18481 catttatcct gttttcaagc ctgatgaatt ttatgagcat atttcttgac tttttccaaa
    18541 ttttgtttgt gaaaattcta actttatggt attataataa catgaaaatg aaatgtaagg
    18601 gcttcaattt agatgattat ttgatattac aacttatttt ctataagaga aaggagttgg
    18661 tagcttgact cttcgttcac attctgaacg gctaatgggg actactatca ctatttcatt
    18721 agtggatgag caagccgata tctttctcca aaaatccttt gacttgctca aagaacttga
    18781 ataccgcttc aacgccaata gtcaagaatc tgagttgatg gaaatcaatt atcaagcagg
    18841 aatagccccc gtcacggttc atccagacct gtttgagctg atttcacttg ggttagagca
    18901 tagcctagcg ctctctagcc acctcaatat cagcattggt cccttaattc aaacctggcg
    18961 tatcggtttt tcagatgcca aggtcgccca gcctcaagaa attgaatcgg tgctgcctct
    19021 aatcaatcct catggtatca agttagattc ttctacttct actgtgtttt taaaacagaa
    19081 aggaatgaag atcgatctag gttgtttagc caaaggatac agtgcggata aggttgccca
    19141 atttcttagg aaagaggggg tgacttctgc cttgatcaat ctgggaggga atatcctgac
    19201 cattggaaaa aatcaggcaa gaggggataa cccatggcaa atcgggattc aagacccagc
    19261 caatcctagg ggaaatcatt taatgaccat ccctgttgtc aataaatctg tcgtgacttc
    19321 aggcatttat gaacgtcacc tgaccgtcga tggacaagat taccatcaca tttttgacag
    19381 tcaaacagga tatcctgttg aaacggaact agcgagccta acaatcatct ctgataaatc
    19441 agtcgatggc gaaatctgga cgactcgact ctttggagaa agacctgctt ctatcctctg
    19501 gcaagtcgaa agtttggagg gcatcgaagt catcctcatc gataaagaag gccacctaag
    19561 ctgttcttca ggaattccta ctctatagag acaactgttt caatggaatt ttaaaattgt
    19621 attatgtaaa aagagaaagg aaatctcatg ttaaaactta ttgctattgt tggaacaaat
    19681 tcaaaacgtt ctacaaaccg tcaattgctt caatacatgc aaaaacactt tactgacaaa
    19741 gctgaaattg agcttgttga aatcaaggcc attcctgtct tcaacaaacc agctgacaag
    19801 caagtacctg ctgaaatatt ggaaattgct gctaaaatcg aagaggctga tggcgttatt
    19861 atcggtactc ctgagtatga tcactctatt ccagctgttt tgatgagcgc tcttgcttgg
    19921 ttgtcttatg gtatttaccc acttttgaac aaaccaatca tgattacagg tgcttcttac
    19981 ggtacgcttg gttcatctcg tgcccaattg caacttcgtc aaatcttgaa tgctcctgaa
    20041 atcaaggcaa atgttcttcc agatgaattc ttgctctcac actctcttca agcatttaac
    20101 ccaagtggcg acttggttga ccttgatgtt atcaagaaat tggatgccat ctttgatgac
    20161 ttccgtatct ttgtaaaaat cacagaaaaa ttacgtaatg cacaagaatt acttcgcaaa
    20221 gatgctgaag actttgactg ggaaaatttg taagatagga gaccaaaaag aatgaaattt
    20281 gttggacttg ttggatcaaa ctacgatcaa tcatataacc gtaagctctt ggaatttatc
    20341 cgtcgctact tcaaactcaa atttgaatta gaagttcttg aaattgacga agttccaatg
    20401 tttaaccaag atgaaaaatg ggatgaaagt ttccaattac gtttcttgta taacaagatt
    20461 acacgtgctg atggtgtcat tatcgccact cctgagcaca accacactat ctcagcttca
    20521 ctcaaatctg tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt
    20581 atgattgtgg gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt
    20641 aaaatccttg atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt
    20701 ggtaaagcta aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa
    20761 ttccttgaaa cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa
    20821 aaaccaaaac caatcgaatc agaagactta gattgtggaa aaccaatcgc tacaaccatc
    20881 acagaagttg accctgacga tccagaatgg gtagaaaaag ttgcagcaat cactggtgct
    20941 gtttctggtg atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat
    21001 atgttcttga aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc
    21061 tactacaaca acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa
    21121 tcaggtagcc gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta
    21181 gagtgggtta tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca
    21241 ggttctcctg caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga
    21301 tcatacgctg gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta
    21361 aaagagactg gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggagat
    21421 catggagacg ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt
    21481 ggtgacgcag acgctacagc cggtgcaagt tactaatgat agtttttagg aaccattatg
    21541 gttccttttt tagcgaaaaa agactaacaa acacaaactg ctagtctctt tgttatcgtt
    21601 tcatctatcc ttgttaaaag aaagaatact tatactcaat gaaaatcaaa gagcaaacta
    21661 ggaagctagc cgcaggctgt acttaagtac ggcaaggtga agctgacgtg gtttgaattt
    21721 gattttcgaa gagtattatt atgaatttgg gtcatcaaga ctacggccat gtaatccttt
    21781 ttcacgttgt acttggcgta gtttttctgg tgtaacatcg tttccttctt cgtccacaat
    21841 tttgattcct tcaatgtggt gacgaacagc gcggcgataa ccttcgatgt actcctcacg
    21901 tagtttggct tgttccactt tttcttctgg tgttaagcct tctgtttttt tctttttagc
    21961 aagctcattg atacgagcaa tttttttcgg atccatttct tctcctttgt aataagataa
    22021 agtccgttta tcaaatttta tttagtgtta atttggttaa aacaagcaaa cttagctgct
    22081 ttcttggtta gttgtgacaa gattgccaaa cgattttgac ggacagcctg atcttcagcc
    22141 attaccatag tattttcaaa gaaagcatca atgactgggc taagcgcaaa gagttgtttc
    22201 aattgctgac ttgcaggtcc tgataaaacg agtgtttcta ctgcttctgc caaagctttt
    22261 tcttggtcat tctcaaatag tgctgaatca actgtagcaa ccccttctgc cttctcagcc
    22321 aggttaaagg cacgagaaag tgattcaaca gatggtttaa aatcttcttc cttgcttact
    22381 tctacgagag cacttgctgc ttccaacata tctgccacaa caaagtttga acctgcaaga
    22441 actgcttcct tgatatcttt tggagtagag cccatcatct tatcaacacg agccttgata
    22501 aagtccataa cctctgcttt attttcataa gtcaaactgt caaatttcaa tgcataaagg
    22561 ctatcaatca gctcatccat agcaatgtgc caaccaaagg catccaagat acgaaccaca
    22621 ccttgagttg cacgacgaag ggcataaggg tcattagaac ctgatggaat caatcctact
    22681 gagaagaaac tcaaaatcgt atccaatttg tctgcaatgg ctagaactgc gccgaccttg
    22741 ctctctggaa gttctccttc agctgatgta ggcatgtagt gttcacgaat agcagctgcc
    22801 accgctggag tttcaccagc aagaagggtg tatttttcac ccataattcc ttggagttcg
    22861 tcaaattcac caaccatacc tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca
    22921 aggtcaactg tttcatccac tgacaaacca gctttttctg ccaaaagtac agtgatttga
    22981 cccgtacgaa tcatgtgttc acgaagggaa ccaatcttct catggaaggt gacattgttt
    23041 aatttttcaa caagatctga aatcaccaat ttttggtctt cacgccagaa gaattctccg
    23101 tcttccaagc gggctaccaa gactttttca tttcctttga tgacattttt caaacgctct
    23161 gcgtttccgt tacgaacaga aatgaagttt ggcaagagtt ttccatcttg atcacgaaca
    23221 acaaagtaac gctggtgttc cttcatagaa gtcaccaaga cttcttctgg aacttcaagg
    23281 tatttagcat caaaacttcc catgaaggca gttgggtatt caaccaaatt caagacttca
    23341 ttcagcaaat ccgcatcaat ttcgatacgt acaccatgtt ttgcctcaat ttccttgatt
    23401 tggtcaacaa tcatttgctc acgttcacat ggatctgcga ttacaaactg cttacgaagg
    23461 tcttcttcat agctcaatgc tgactgaatt ttggtttctt tccccaagaa acgatggcca
    23521 cggctcacac gacttccctt gatatcaagg aaatccaagt caaactcttg ctcatccaag
    23581 agaacagtta aagtgtgaac agggcggatg tattcaaagc tatttccagc ccagtgcatg
    23641 ctgacaggga aagtcaatga cttcaagaca tctacaacac caggaacaat ggcttcaact
    23701 gcttgaccaa cttcttcctt agtgacatag acatattctt cacccttgat ttcacggaat
    23761 tcgatatctt caacagtcaa gccttttcca cggacaaatc cttgagctgc tttggtgaag
    23821 tttccatcac tatctaaggc aattttcttt gctggacccc tgaaatcttc tgtcaaatca
    23881 gactgtttgt ctgcaagacc agttacacga acagccaaac gacgtggtgt tgagaaggtt
    23941 tgaatggctt caaaagacag gcgttttccc ttgaggaagg ctgccatttt ttcgcctagt
    24001 tgtttttcac ttggcgtaac aacataggct ggtaattctt caagaccgag ttctactaat
    24061 aagttttttg tcatgttttt tctccgaaaa gtatcttttt attttccagt ttgccttatg
    24121 tgattggcac gcaagcaacc aacttcgttg tttcattgct aaaattggag ccaaaagtct
    24181 ccaattttgc ctagtatcct tagtttcact aaggatacct tcatcactgc ggcaactggc
    24241 tcagggctca ctctgttcgc ccattttcgt aatttgtcac tctctttatt ctgcgtcttc
    24301 tgctaggagt ttagctcttg tttcttcatc caaaagtggg tagcctaggc gtttgcgttc
    24361 tgcgacaaag gttttggcta cgacacgggc caagttacgg atacgagcga tatagcctgc
    24421 acgctctgtt acagatacgg caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt
    24481 gagaacatag tcataggcag ggtgtaccaa gccttcttct aatgcacgac cagcttcttt
    24541 ttcaaactta tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa atgaatattt
    24601 tgagtgctca tactcaggct ggataaagat ttctccgtat tttacaccat cagcccactc
    24661 gatatcatag acagagtcta cttcttgaat gtaagaagcc aagcgctcca aaccataggt
    24721 aacttccgca gtcacagggc cagttgccaa tccaccgact tgttggaaat aagtgaactg
    24781 agtgatttcc attccgtcaa gccaaacttc ccaaccaaga ccagctgaac cagttgatgg
    24841 gttttcccag ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga ttcccaattt
    24901 ttccaaagac tcaaggtaaa gttcttggat atttgatgga gaaggcttca tgaccacctg
    24961 gaattggtgg tgttggtaga gacggttagg gttttcccca taacgaccgt cagcaggacg
    25021 acgtgatggc tctacataag ctgcattcca tggctcaggt ccgatagcac gaaggaaagt
    25081 gtaaggactc attgtccccg cacctttttc attatcataa gcctgcataa gcatacaatc
    25141 ttggtcattc caaaattgtt gcaaagtcaa aataatttct tgaaatgtta atttcttaga
    25201 cattgaatac tccttaataa aaaataattt cttgcgacac gagtctgcct cgtcgattat
    25261 acgaggcaag acgagttagt actgcgtcta gctcaggcac tctagtgctg agcgtagggc
    25321 agtccgactg taaggaggtt tctgccactg acgcagtgaa atgttaattt cttagacatt
    25381 gaatactcct taataaaaaa taattacttg cgacacgagt ctgcctcgtc gatcatacga
    25441 ggcaagacga gttagtactg cgtctagctc aggcactcta gtgctgagcg tggagcagcc
    25501 cgactgtaag gaggtctctg ccactgatgc agtgaaatgt taatttctta gacattgttt
    25561 actcctttag tttttatatt tctttttcaa ttaatttctg ctgaacaaac caatctaaca
    25621 agggaaaagt tttttctgta tttgcccata aatgataaga atctaagacc gcttgtacac
    25681 tacctaaatt ttctaccaac ttatctactg tcaaaaagct gcgccagtat tcataaaaaa
    25741 tacttgcata gttcttatca taagtcgaag aaccaaaatc attcaatgaa tgccaactat
    25801 acttcttctg aaaaagttct acgagagatt gattacaaat tttttccgct tgaaattcct
    25861 cttctgtcaa gaaatacttg cgactaatat attcaaccat cccctcttcg aaccagatat
    25921 aagagtcata accatcaaaa tcatctaaaa aatgtccgac caatgagcta actcatgtcc
    25981 tacaatctgt aagaggaaat tttcagataa agaatgatag tgactttcta ttgcttgagt
    26041 ttgctcagaa cactcgtagt ccatcaactg atacaaatac aattctttcc aaacagctaa
    26101 atcaggcgtc ataaccactc gtctattatt tgtataggtt ggaacagcac tttccctaat
    26161 aatctgtgta gcagcattaa aatctgacca aataacagct tgcggtaaat cacagactgc
    26221 aagttcattc tttaaaaaag attgataatc tttcaacttt tcagtatttc ttactacaaa
    26281 atcacgaaat gtagctagtt gactatcgtc ttttacaaga taaaggttgt ccacggctct
    26341 cctttctttc acacaataga ctgaataagc aaaagaacca catctgacaa tcctaggttg
    26401 atttaaccta ggggcgtcag aaacgcggtt ccaccctaat ttattacttc attgtttttg
    26461 aaaatactac agaaagcgcc agccctttat tttgccctac ttggctccca ctatcccaag
    26521 ctcgcttgaa gaacgccata agactttctt tcctgctgac tattatatca aaaattagaa
    26581 taatgtacaa ttctttttat gattttctag aaatccacat catcaactcg tggagcaccc
    26641 gattgaacag caatcgtttt taaggtttct ctctcctcat gactcagttc aattccaaag
    26701 caatcaagat tagcttgaat acgagaggtt gtgacagatt ttggaagtgg taaaaatcct
    26761 tctgccaagc tccaggccaa ggctatctga gcaaccgatt ttccgtgatt tgctgctatt
    26821 tcttggactt gcttgctatc aaacagttct ccttgtccaa aaggccccca agcttccaat
    26881 aaaattccct tttcacgaca gtaagctacg acttgatctt gatacacacc tggcgccaag
    26941 cgaacttgat tgaccgcagg aacgatagtt gcagtttcaa gcaaggcatc caaatgatgg
    27001 ggaagaaaat tgctaacgcc gatagcacgg attttccctt cttgatagag gtcttccatc
    27061 gctctccaaa cttccgcatt gcgagttttc catgcgtcat tttctctgag cggttttggg
    27121 ttcggccaat gaatcaaata caaatccaaa taatccaagc ccagtttttc tatagatttt
    27181 tccaaagctt gacgagtttg ctcataggtt tgctgactat tccaaagctt ggtagttacg
    27241 aacatttctt cacgtggaac tccgctatct ttgattgctt gaccaacact ttcttcattc
    27301 tgataaatcg ccgccgtatc aatatgacga taaccagcct tcaaggcttc taacactgca
    27361 cgataggctt cttctccatc cttagcctta aaagttccaa atcccaatac tggaatttct
    27421 actccattat ttaattgata ggtattcata tgcttctcct tctattttat tcatcaatta
    27481 tagcataaaa cattttttaa gtagtactat attctgtaaa aaataagtct gtcaaatatc
    27541 taacagacct attttttctt gcttattcat cacgactata gtacagctca tgagctttta
    27601 ggcgagtatt gagcatctct ggtatggtca caaaggtata accttgattt ttcaaatact
    27661 caatgaccct tggcaaggca ttgactgtcg gactgtgaat atcatgcatc aaaacgatag
    27721 agccattagc tacttgatgc tgaatttctg tcaaaataga tgcttcattt ttactcttcc
    27781 agtccagact atccacatcc cacatgataa agctcaaatc caagctattg cgaatatcat
    27841 ctgtaatagc accataaggt ggacgcatga gtttagaact agaacccagc actttagtta
    27901 gcacatcctc agtatcagta atctgctttt tagcttcatc aagagagagt tgcgagagaa
    27961 tcggatggct ccagctatgg tttccaacaa catgaccttc agattttatc ctcttcacca
    28021 agtcctcatt cccagaaaca tttttcccaa gcacaaagaa agtcgcttta atatcatatt
    28081 tagctagggt ctctaatacc tgcggggtcg ttgctggatt tggaccatca tcaaaggtta
    28141 gagcgacaac tttttgatgt ttcttatcaa agtaagattg gtacaaggcc gcatcttttt
    28201 cgagtaagta cgaagattgg ataacatcaa agaaagcaga tactggcaag gctatctctt
    28261 ctaaattttc aaccacagga cttggataaa ggataatctg actatccttg taatcaaaat
    28321 tccatgcaga caagtcttgg tcagagaagt tttttacaat ctgctcactc tggtcttgct
    28381 ctattttttt atcctctatg aaggaggtca actcttttat cagctgttcc ttagccttac
    28441 tagcatctga aaacagttgg tcaagtgtaa aaggttgccc atcttctgtt aagtgaacct
    28501 ttcctagact ggtcttttca gtctcttcaa tcttaaaagc aactaaatca tagatttgtt
    28561 tggtcacatt acgattaacg actcccttta aacctgactc ttcttgctct gtatagtaga
    28621 aaacaagatt gtccttactt tctagcttgt ccttaacatc ttgatttatc agctccctaa
    28681 cagaggaaat cactttctcc ccttggagag gataataagc aatcacttcg gcttgcccct
    28741 gacgaaaatg ctccttctga tttccctcac tcaattgatc atctttctct tttttgagcg
    28801 attcaatctt ttgctcaaaa cttttttgct ggtagatctt aaaagcaata aatccgccta
    28861 ataggcaaat agaaattgct aaaataccaa tcaaagccaa taatacatgt ctcgttttcc
    28921 cgtgtctgcc acgtcctagt ctacttttat tcatatttat atcataacaa atttgagcaa
    28981 taatttcaat gatacaattt ggcaatccac aattcctcta gcaagccaaa gcatcttaca
    29041 tcaactccaa catccgtatc tgtagagatt atgtagtttt ctatctatgg aatttctagt
    29101 agattgaagc tagaatagta gacctatact tctaaaacat tgttagaaat cgatttgact
    29161 gtcctgatcg atttgtcctg ttctcatttc attttactat aaaactatga aaagctaggt
    29221 catctgagaa gtaattacta tcgacaattg cgatagcgta tactagtgcg atttgtttgt
    29281 agctctggtg ggtatcacct tcacgttggc gaattttttc taggttttga ttaacctgac
    29341 tgcacagata aggccatagg cgattgatga aaaaattctg atgatgaacc tgaatttcga
    29401 tgataacttg gattccatta tctaactccg ccaagacgtc tatactagta tagaaatctt
    29461 acgctgagta gggcatggaa ggcaagacgt gaatgttact tccctccaaa atcgtcacat
    29521 tttttgctgg caagtccagc atatcgcgga taaattgaca aatcattagt tgggctgata
    29581 cctagatgtc tgagaatcct ccttttcctc cttctattat agcacatttt taaatctagg
    29641 tttgctagat tctatgcttc tatctattta ttcggaaaag aaagctaata gcttatttga
    29701 aattttctcc tctctgttgg atagtcccgt ctatctatgt tataatgaaa gaaggattta
    29761 aaatcggaaa aggagtattt atgctaaaat taggtgtcat cggaactggc gctatcagcc
    29821 atcatttcat agaagcagcc cataccagtg gagaatacca actggtcgca atctattcta
    29881 gaaaactaga aactgcagca acctttgctt ctcgctatca gaatatccaa ctattcgatc
    29941 aattagaagt cttcttcaaa tcttcttttg atttagtcta tatcgccagt ccaaactcct
    30001 tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa acatgttatt ctcgaaaagc
    30061 cagctgtctc tcaaccacaa gaatggtttg atttgattca aacagctgaa aaaaataact
    30121 gttttatctt tgaagcagct cgtaattacc acgagaaagc ctttactact atcaaaaact
    30181 ttttagcaga taagcaagtg ttaggagcag atttcaacta tgccaaatac tcttccaaga
    30241 tgcctgactt gttggctgga caaacaccaa atgtcttttc agaccgtttt gctggtggag
    30301 ccctcatgga tttggggatt tatcccctct atgctgctgt tcgtcttttt ggaaaagcca
    30361 atgacgcgac ttaccatgct caacagcttg acaatagcat tgacctaaat ggagatggta
    30421 tcctcttcta cccagactat caagttcaca tcaaggctgg aaaaaacatc acttccaatc
    30481 ttccttgcga gatttataca acagatggaa ccttgactct caacacgatt gagcatattc
    30541 gctcagctat ttttaccgac caccaaggaa atcaagtcca gcttcctatc caacaggctc
    30601 ctcatacgat gactgaggaa gtcgctgcat ttgcacacat gatccagcaa ccagatctga
    30661 atctctacca aacttggctg tatgatgcag gctctgttca tgagctacta tataccatgc
    30721 gccagactgc tggcattaga tttgaggcag aaaaatgaaa accaaactac cgactgaatg
    30781 gcaagaactg agtgaccaac tcggtttcca agaattcacc cccattcaaa ctcaactatt
    30841 tgagcccctt cttgctggag aaaacctcct aggagtgagc caaaccggaa ctggtaagac
    30901 cctagcgtat ctcctaccaa gtcttctcag actacaaaag aaaaaagccc aacaactctt
    30961 gattctagca ccaaatacgg aacttgctgg acagattttt gatgtatgta aaacgtgggc
    31021 agaagctatc ggattgactg ctcaactctt cctttcaggt tcaagtcaga aacgacaaat
    31081 tgaacgtctt aaaaaggggc cagaaattct gattggaact cctggccgta tctttgaact
    31141 cattaaactt aaaaaaatca agatgatgaa tgtggaaacc atcatcctgg atgaatttga
    31201 ccaattacta gatgattctc agattcactt tgtagaaaaa atcactcact acgcacctcg
    31261 tgaccaccaa ctggtctaca tgagtgccac gaccaagttt gaccaagaaa agattgtgcc
    31321 gaatacacgc actattgatc tctctgatca aaaattggac aatatccagc atttctacat
    31381 gcaggtagac caacgtcatc gagtggatat gctacgaaaa ctggctcatg tagaggattt
    31441 ccgtggtcta gtcttcttca atagcctgtc agaccttggg aatgccgagg aaaaactaca
    31501 gtatcgtgat atattggctg tttccctcgc tagtgatgtt aatgttaaat ttagaaaaat
    31561 catcttagaa aagtttaaag ataaccaact aaccctgctt cttgcaactg accttttggc
    31621 tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac tttgatattc ctagagatag
    31681 cgaaacctac acacaccgtg ctggccgtac tggccgtatg ggcaaggaag gatatgttat
    31741 tactctcgtc actcatccag aagaaatcaa aaaacttaaa aagtttgcaa gtatccgaga
    31801 aattgtccta aaaaaccaag aactctatat caaataaagc cccattgctc tatacacatc
    31861 cagtgtatgg agctttttta taagcaattc aattttatac tcatctgctt tcaaaaagca
    31921 ttctagtcca tctctgatta acgatggact ttatcacctc cttctccagt ccttgtatga
    31981 catcttgaag ttgattcatg acatcttcca aagttcgaaa ggctttattc ttaaatccac
    32041 gtttacgaat ctctttccac acttgttcaa tggggttcat ctctggtgtg tatggaggaa
    32101 taaatgcaaa gccaatatta gtcggaatct ttaaggtact tgatttatgc catatagcat
    32161 tgtccataac gagtaaaaaa taatcatctg gataagcttg tgaaagctct tctaaaaaga
    32221 cgttcatcca ctcagtatta catccaccag ctcttaagaa aaatgattcg cctgtatggg
    32281 catcaacagc tccataacaa tagcgaaatt ctcgtatata gtgactatgg acatgtggac
    32341 ctactcctat tggagaccaa caagatccca gtttactgat tctaccgaaa ccagcatcat
    32401 cttggtacat caagcgaacc ttatgaaaag gtctactggt tttaaagcgc tttcttgtgt
    32461 attgcccccc aaaagttaga caattaattt atccgaagga tttagttctg tattgcacag
    32521 ggctaagtcc ttttagtttt atcttaatt
//
LOCUS       DAVKHZ010000013        32450 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000013 DAVKHZ010000000
VERSION     DAVKHZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.013"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            556..1008
                     /locus_tag="VWW99_000598"
     CDS             556..1008
                     /locus_tag="VWW99_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9538478.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFIYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1027..2364
                     /locus_tag="VWW99_000599"
     CDS             1027..2364
                     /locus_tag="VWW99_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9538479.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2443..3327
                     /locus_tag="VWW99_000600"
     CDS             2443..3327
                     /locus_tag="VWW99_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9538480.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3327..4160
                     /locus_tag="VWW99_000601"
     CDS             3327..4160
                     /locus_tag="VWW99_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9538481.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4355..5458
                     /locus_tag="VWW99_000602"
     CDS             4355..5458
                     /locus_tag="VWW99_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9538482.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            5632..6330
                     /locus_tag="VWW99_000603"
     CDS             5632..6330
                     /locus_tag="VWW99_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9538483.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            6349..7866
                     /locus_tag="VWW99_000604"
     CDS             6349..7866
                     /locus_tag="VWW99_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9538484.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            7991..9319
                     /locus_tag="VWW99_000605"
     CDS             7991..9319
                     /locus_tag="VWW99_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9538485.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWVFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            9571..10437
                     /locus_tag="VWW99_000606"
     CDS             9571..10437
                     /locus_tag="VWW99_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9538486.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            10453..11292
                     /locus_tag="VWW99_000607"
     CDS             10453..11292
                     /locus_tag="VWW99_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9538487.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            11564..12016
                     /locus_tag="VWW99_000608"
     CDS             11564..12016
                     /locus_tag="VWW99_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9538488.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            12113..12457
                     /locus_tag="VWW99_000609"
     CDS             12113..12457
                     /locus_tag="VWW99_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538489.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIACSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            12741..13385
                     /locus_tag="VWW99_000610"
     CDS             12741..13385
                     /locus_tag="VWW99_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9538490.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            13554..14471
                     /locus_tag="VWW99_000611"
     CDS             13554..14471
                     /locus_tag="VWW99_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9538491.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            14489..15373
                     /locus_tag="VWW99_000612"
     CDS             14489..15373
                     /locus_tag="VWW99_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9538492.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(15389..16240)
                     /locus_tag="VWW99_000613"
     CDS             complement(15389..16240)
                     /locus_tag="VWW99_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9538493.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            16440..18497
                     /gene="pbp2b"
                     /locus_tag="VWW99_000614"
     CDS             16440..18497
                     /gene="pbp2b"
                     /locus_tag="VWW99_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9538494.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            18508..19104
                     /gene="recR"
                     /locus_tag="VWW99_000615"
     CDS             18508..19104
                     /gene="recR"
                     /locus_tag="VWW99_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9538495.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            19280..20323
                     /locus_tag="VWW99_000616"
     CDS             19280..20323
                     /locus_tag="VWW99_000616"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9538496.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            20407..21780
                     /locus_tag="VWW99_000617"
     CDS             20407..21780
                     /locus_tag="VWW99_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9538497.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            21767..22378
                     /locus_tag="VWW99_000618"
     CDS             21767..22378
                     /locus_tag="VWW99_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9538498.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            22469..23173
                     /locus_tag="VWW99_000619"
     CDS             22469..23173
                     /locus_tag="VWW99_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9538499.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            23392..24765
                     /gene="ftsA"
                     /locus_tag="VWW99_000620"
     CDS             23392..24765
                     /gene="ftsA"
                     /locus_tag="VWW99_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9538500.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            24782..26041
                     /gene="ftsZ"
                     /locus_tag="VWW99_000621"
     CDS             24782..26041
                     /gene="ftsZ"
                     /locus_tag="VWW99_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9538501.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            26046..26717
                     /locus_tag="VWW99_000622"
     CDS             26046..26717
                     /locus_tag="VWW99_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9538502.1"
                     /translation="MNVKENTELVFREVAEASLSANRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            26727..27266
                     /locus_tag="VWW99_000623"
     CDS             26727..27266
                     /locus_tag="VWW99_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9538503.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            27266..27529
                     /locus_tag="VWW99_000624"
     CDS             27266..27529
                     /locus_tag="VWW99_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9538504.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            27526..28311
                     /locus_tag="VWW99_000625"
     CDS             27526..28311
                     /locus_tag="VWW99_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9538505.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELNLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLFQDKGQTKKEKKKITVQLLLSK"
     gene            28320..29108
                     /gene="divIVA"
                     /locus_tag="VWW99_000626"
     CDS             28320..29108
                     /gene="divIVA"
                     /locus_tag="VWW99_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9538506.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            29456..32248
                     /locus_tag="VWW99_000627"
     CDS             29456..32248
                     /locus_tag="VWW99_000627"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9538507.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT         9887 a         5804 c         7104 g         9655 t
ORIGIN      
        1 aaactaattt agagaatgaa atatagagta tttctctctt aaaagttttt ggtgaaacga
       61 gatgtagaaa ggagatttag ccaaagagtc tattagtgct agaataatag attagaatta
      121 ttttagaaaa acgaagtgag cagcttataa attcaagtcc ccaaatagat tcatactagt
      181 atcttttgca aaaaataaag ggcgacttcc ttcatgaata tcaatttcat ctataaggaa
      241 ggtagctaat tgaactaact tatttattct gtttgtcgct agaaaaatca gacctccttg
      301 tgaagattga ggagatactg aatgaaaatc aaagaagaaa ctagcaagct agtagcagat
      361 tgcccaaaac accgctttga ggttgtagat aagactgacg tatataatcc aaggtgaagc
      421 gactgtggtt tgaagagatt ttcaaagagt ataggctaga gagtagtgtt tttatgtcct
      481 tctagtagaa aatgctagac agaagaatgg ggaacttgga taggaaaaat agattgagaa
      541 aggaggttag aagagatgat tattacaaaa attagccgtt taggaactta tgtgggagtc
      601 aatccacatt ttgcaacatt aatagatttt ctagaaaaaa caggactaga aaatttaaca
      661 gaaggttcga ttgctatcga tggtaatcga ttgtttggga attgctttat ttatctagca
      721 gatggtcaag caggggcttt ctttgaaaca caccaaaaat atttggatat tcatttagtt
      781 ttggaaaacg aagaagccat ggctgttaca tcgccggaaa atgtaagcgt tacccaagaa
      841 tatgatgaag agaaagatat tgaattatac acagggaaag tggaacagtt ggttcatttg
      901 agagctggtg aatgcctcat cacttttcca gaagatttac atcaacccaa ggttcgtata
      961 aatgatgaac ctgtgaaaaa agttgtcttt aaagttgcga tttcttaatg tagaaagaga
     1021 agaacgatga aaaaaatgag aaagttttta tgtctagctg gaattgcgct agcggctgtt
     1081 gccttggtag cttgttcagg aaaaaaagaa gctacaacta gtactgaacc accaacagaa
     1141 ttatctggtg agattacaat gtggcactcc tttactcaag gaccccgttt agaaagtatt
     1201 caaaaatcag cagatgcttt catgcaaaag catccaaaaa cgaaaatcaa gattgaaaca
     1261 ttttcttgga atgacttcta tactaaatgg actacaggtt tagcaaatgg aaatgtgcca
     1321 gatatcagta cagctcttcc taaccaagta atggaaatgg tcaactcaga tgctttggtt
     1381 ccgctaaatg attctatcaa gcgtattgga caagataaat ttaacgaaac tgccttaaat
     1441 gaagcaaaaa tcggagatga ttactactct gttcctcttt attcacatgc acaagtcatg
     1501 tgggttagaa cagatttgtt aaaagaacat aatattgagg ttcctaaaac ttgggatcaa
     1561 ctctatgaag cttctaaaaa attgaaagaa gctggagttt atggcttgtc tgttccgttt
     1621 ggaacaaatg acttaatggc aacacgtttc ttgaacttct acgtacgtag tggtggagga
     1681 agcctcttaa caaaagatct taaagcagac ttgacaagcc aacttgctca agatggtatt
     1741 aaatactggg ttaaattgta taaagaaatc tcacctcaag attctttgaa ttttaatgtc
     1801 cttcaacaag ctaccttgtt ctatcaagga aaaacagcat ttgactttaa ctctggcttc
     1861 catatcggag gaattaatgc caacagtcct caattgattg attcgattga tgcttatcct
     1921 attccaaaaa tcaaagagtc tgataaagac caaggaattg aaacctcaaa cattccaatg
     1981 gttgtttgga aaaattcaaa acatccagaa gttgctaaag cattcttaga agcactttat
     2041 aatgaagaag actacgttaa attccttgat tcaactccag taggtatgtt gccaactatt
     2101 aaggggatta gcgattctgc agcctataaa gaaaatgaaa ctcgtaagaa gtttaaacat
     2161 gctgaagaag taattactga agctgttaaa aaaggtactg ctattggtta tgaaaatgga
     2221 ccaagtgtac aagctggtat gttgactaac caacacatta ttgaacaaat gttccaagat
     2281 atcattacaa atggaacaga tcctatgaaa gcagcaaaag aagcagaaaa acaattaaac
     2341 gatttatttg aggctgttca gtagatgtaa aagactagaa aataggtggg ctagtgatct
     2401 gaaaagctct agcccaattt tgtaaaagaa gggagaagga gaatggttaa agaacgtaat
     2461 ttaactcgct ggatatttgt tttgccagct atgattatcg taggattact ctttgtttat
     2521 ccgtttttct cgagtatttt ttatagcttt accaataagc atttgattat gcctaattat
     2581 aaatttgttg gtttggctaa ctataaagct gtgctatcag atcccaactt ctttaatgcg
     2641 ttctttaatt caattaagtg gaccgttttc tcattagttg gtcaagtttt agtagggttt
     2701 gtgttggctt tagctcttca cagagtacgc cacttcaaga aattatatag gaccttattg
     2761 attgttcctt gggcatttcc taccatcgtt attgccttct cttggcagtg gattctaaac
     2821 ggggtttatg gctacttacc taatctaatc gtaaaattag gtttaatgga acatacacct
     2881 gcatttttga cagatagtac atgggcattc ctatgtttgg tgtttatcaa catttggttt
     2941 ggagcaccaa tgattatggt taatgtgctt tcagctttgc aaacagtacc agaagaacaa
     3001 tttgaggctg ctaagataga tggtgcttca agttggcagg tgttcaagtt tatcgtcttt
     3061 ccacatatta aagtggttgt aggacttcta gttgttttga gaactgtatg gatctttaat
     3121 aactttgaca ttatctacct cattactggt ggtggaccag ccaatgctac aacgacgctt
     3181 ccaatttttg cttacaacct gggctgggga actaaattgt tgggtcgtgc ttcagcagtt
     3241 acagtattgc tctttatctt cttggtggcg atttgcttta tctactttgc tatcatcagt
     3301 aagtgggaaa aggagggtag aaaataatga agaagaaatc cagtatttat ttagatattc
     3361 tctcacatgt acttttagtt ggtgcgacca tcgttgcaat tttcccattg gtatggatta
     3421 tcatatcttc tgtcaaaggg aaaggggaat taactcagta tccaacacga ttttggcctg
     3481 aacagtttac attagattat ttcactcatg ttatcaacga tttgcacttc attgataaca
     3541 ttcgaaacag tttaatcatt gccttggcta caaccctcat tgcgattatt atttctgcta
     3601 tggcagccta tggtattgtt cgattctttc ctaaattggg agcaatcatg tcgagactac
     3661 tcgtcattac ctacattttc ccaccaattt tgttagcaat tccctattca attgccattg
     3721 ctaaagttgg gttaacaaat agtttatttg gcttgatgat ggtttatcta tcttttagtg
     3781 ttccatatgc agtttggctc ttagttggat ttttccaaac agttccaatt ggaattgaag
     3841 aagcggctag aattgatggt gcaaataaat ttgttacgtt ttataaagtt gtgctaccga
     3901 ttgtagcacc aggtattgta gcaacagcta tttatacatt tatcaatgct tggaatgaat
     3961 tcctgtatgc cttgattttg attaacaata caggaaagat gacagtagca gtagcccttc
     4021 gttcacttaa tggttcagaa atactagact ggggagatat gatggcagcg tctgttattg
     4081 tagttcttcc atcaattatt ttcttctcta tcatccaaaa taagattgca agtggattat
     4141 cagaaggatc tgtgaagtaa tactcaatga aaatcaaaga gcaaactagg aaactagccg
     4201 caggctgctc aaagtacagc tttgaggttg cagataaaac tgacgaagtc agtaaccata
     4261 cgcacagtaa ggcgacgttg acgcggtttg aagagatttt cgaagagtat aagttaaaat
     4321 tagaaagttt attcaataat aaaggagaaa aaacatggtt aaatacggtg ttgttggagc
     4381 agggtatttt ggagctgaat tggctcgcta catgcaaaag aatgatggag cagagattac
     4441 tcttctctat gatccagata atgcagaggc gattgcagaa gaattgggag caaaagtagc
     4501 aagttcctta gatgagttgg tttctagcga tgaagtagat tgtgttatcg tcgcaactcc
     4561 aaataatctt cataaggaac cggttattaa ggctgcacag catggtaaaa atgttttctg
     4621 tgaaaaacca attgcgcttt cttatcaaga ttgtcgcgag atggtagatg cgtgtaaaga
     4681 aaacaatgta acctttatgg caggacatat tatgaatttc tttaatggtg ttcatcatgc
     4741 aaaagaactc attaatcaag gagttatcgg agacgttcta tattgtcata cagctcgtaa
     4801 tggttgggaa gaacaacaac cgtcagtgtc atggaaaaaa attcgtgaaa aatcaggtgg
     4861 tcacttgtat caccacatcc atgaattgga ttgcgttcaa ttccttatgg ggggcatgcc
     4921 tgaaactgta accatgacag gtggaaatgt ggcccatgaa ggtgaacatt tcggtgatga
     4981 agatgatatg atttttgtca atatggaatt ttctaataag cgttttgcct tgttagaatg
     5041 gggttcagct tatcgttggg gtgaacatta tgtcttaatc caaggaagca aaggtgccat
     5101 ccgcttagac ttattcaact gtaaaggaac tcttaagcta gatgggcaag aaagctattt
     5161 cttgattcac gaatcgcaag aagaagatga tgatcggact cgtatctatc atagtacaga
     5221 gatggatgga gcaattgctt atggtaaacc aggtaaacgt actccattat ggctatcatc
     5281 tgtcattgat aaagaaatgc gctatctgca tgagattatg gaaggagccc cagtatcaga
     5341 agaatttgca aaacttttga caggtgaagc tgccctagaa gcaattgcta ctgcagatgc
     5401 ttgtacccag tctatgtttg aagatcgcaa agtaaaattg tcagaaattg taaaataaat
     5461 tttggtattc tcctatttat aggtcgactt gctcctctga aagtactttt agaggagctg
     5521 tttgactttg ctagtttttg aaactgaaat ctattatact acaaactatt gaaagcgttt
     5581 taattttaag gtataataat ctcatagaaa taaagaaaag gaggaaagag gatgccacag
     5641 attagcaaag aagccttgat tgagcaaatc aaagatggaa tcatcgtttc ttgtcaggct
     5701 cttcctcatg aaccgcttta tacagaagcg ggaggggtga ttcccttgct ggtcaaagcg
     5761 gctgagcaag gtggagcagt cggtatccga gcaaacagtg ttcgcgatat caaggaaatt
     5821 aaggaagtca ctaaacttcc aatcattggg attatcaaac gtgattatcc acctcaggaa
     5881 cccttcatca cggctactat gaaagaagtt gatgaattgg cagaactgga catcgaggtg
     5941 attgctctgg attgtaccaa gcgtgaacgc tacgatggtt tggaaattca agagttcatt
     6001 cgtcaggtta aggagaaata tcctaatcag cttttgatgg ctgatactag tatcttcgaa
     6061 gaagggctag cagctgtaga agcaggaatt gactttgtcg gaacaacctt atcaggctac
     6121 acatcctaca gtccaaaagt agacggtcca gattttgaat tgattaagaa actctgtgat
     6181 gccggtgtag atgtcattgc agaaggaaaa attcatacac cagaacaagc caaacaaatc
     6241 cttgaatatg gagtgcgagg catcgttgtt ggtggcgcca ttactagacc aaaagagatt
     6301 acagaacgct tcgttgctag tcttaaataa gatgtgagag ggagttttat gtttaaagtt
     6361 ttacaaaaag ttggaaaagc ttttatgtta cctatagcta tacttcctgc agcaggtcta
     6421 cttttgggga ttggtggtgc actttcaaac ccaaccacga tagcaactta tccaatacta
     6481 gacaatagta tttttcaatc aatattccaa gtaatgagct ctgcaggaga ggttgtattc
     6541 agtaatttgt cactacttct ctgtgtggga ttatgtattg gcttagcgaa acgagataaa
     6601 ggaaccgctg cgttagcagg agtaactggt tacttagtta tgactgcaac gatcaaagct
     6661 ttggtaaaac tttttatggc agaaggatct gcaattgata ctggagttat tggagcatta
     6721 gttgtcggaa tagttgccgt atatttgcac aaccgatata acaatattca attaccttcc
     6781 gctttaggat tctttggagg ttcacgcttc gttcctattg ttacatcgtt ctcttctatc
     6841 ttgattggct ttgtcttctt tgttatttgg ccacctttcc aacaacttct tgtttctaca
     6901 ggtggatata tttctcaggc gggtccaatt ggaacttttc tatatggatt tttaatgaga
     6961 ctttctggag cagtaggctt acatcatata atttacccta tgttttggta tactgaactt
     7021 ggtggtgttg aaactgttgc aggacaaaca gtggttggag ctcaaaaaat attttttgct
     7081 caattagccg atccggccca ttctggatta tttacagaag gaacaaggtt ttttgcaggt
     7141 cgtttctcaa caatgatgtt cggtttaccg gctgcctgtt tagcgatgta ccatagtgtt
     7201 cctaaaaatc gtcgtaaaaa atacgcgggt ttgttttttg gagttgcttt aacatctttt
     7261 attaccggta ttacagaacc aattgaattt atgtttctat tcgtcagtcc ggttctatat
     7321 gttgttcacg cattccttga tggtgttagc ttctttattg cagacgtctt aaatatttca
     7381 ataggaaaca cattttcagg aggtgtaatc gatttcactt tatttggaat tttgcagggg
     7441 aacgctaaga cgaattgggt tcttcagatt ccaattggac ttatttggag tgttttgtat
     7501 tatattattt ttagatggtt cattactcaa ttcaacgttc taacgccagg acgaggagaa
     7561 gaagtagatt ctaaagaaat ttctgaatcc gcagattcaa cttcaaatac tgcagattat
     7621 ttaaaacagg atagcctaca aattatcaga gccttgggtg gatcaaataa tatagaagat
     7681 gtagatgctt gtgtgacacg tttacgtgta gctgtaaaag aagttaatca agttgataaa
     7741 gcacttttaa aacaaattgg tgcagttgat gtcttagaag tgaagggtgg cattcaagca
     7801 atatatggag caaaagcaat cttatataaa aatagtatta atgaaatttt aggtgtagat
     7861 gattaagtac ttactgactt aataaaaaac agaggagagt gatggatgag taggatgaaa
     7921 tgaaatcgca tacaagaaat aaagaactca ttatccaagt tggatacgct tattacatag
     7981 gagaatacaa atgaaattta gaaaattagc ttgtacagta cttgcgggtg ctgcggttct
     8041 tggtcttgct gcttgtggca attctggcgg aagtaaagat gctgccaaat caggtggtga
     8101 cggtgccaaa acagaaatca cttggtgggt attcccagta tttacccaag aaaaaactgg
     8161 tgacggtgtt ggaacttatg aaaaatcaat catcgaagcg tttgaaaaag caaacccaga
     8221 tataaaagtg aaattggaaa ccatcgactt caagtcaggt cctgaaaaaa tcacaacagc
     8281 catcgaagca ggaacagctc cagacgtact ctttgatgca ccaggacgta tcatccaata
     8341 cggtaaaaac ggtaaattgg ctgagttgaa cgacctcttc acagatgaat ttgttaaaga
     8401 tgtcaacaat gaaaacatcg tacaagcaag taaagctgga gacaaggctt atatgtatcc
     8461 gattagttct gccccattct acatggcaat gaacaagaaa atgttagaag atgctggagt
     8521 agcaaacctt gtaaaagaag gttggacaac tgatgatttt gaaaaagtat tgaaagcact
     8581 taaagacaag ggttacacac caggttcatt gttcagttct ggtcaagggg gagaccaagg
     8641 aacacgtgcc tttatctcta acctttatag cggttctgta acagatgaaa aagttagcaa
     8701 atatacaact gatgatccta aattcgtcaa aggtcttgaa aaagcaacta gctggattaa
     8761 agacaatttg atcaataatg gttcacaatt tgacggtggg gcagatatcc aaaactttgc
     8821 caacggtcaa acatcttaca caatcctttg ggcaccagct caaaatggta tccaagctaa
     8881 acttttagaa gcaagtaagg tagaagtggt agaagtacca ttcccatcag acgaaggtaa
     8941 gccagctctt gaataccttg taaacggatt tgcagtattc aacaataaag acgacaagaa
     9001 agtcgctgca tctaagaaat tcatccagtt tatcgcagat gacaaagagt ggggacctaa
     9061 agacgtagtt cgtacaggtg ctttcccagt ccgtacttca tttggaaaac tttatgaaga
     9121 caaacgcatg gaaacaatca gcggctggac tcaatactac tcaccatact acaacactat
     9181 tgatggtttt gctgaaatga gaacactttg gttcccaatg ttgcaatctg tatcaaatgg
     9241 tgacgaaaaa ccagcagatg ctttgaaagc cttcactgaa aaagcgaacg aaacaatcaa
     9301 aaaagctatg aaacaatagt ccttagttat tctataaaaa gtagtttttt aaagaaccta
     9361 agagtgtata cccccttttc cctctacaca gatagtgtaa gaaaaggggg cttttgttta
     9421 aaatgtaaga aactgtcacg aaattaaaat gaagttctta cataagcgaa ccataaaaaa
     9481 tttcattttg attttaaaac agttcaagaa agtcaaaaaa ttattctatt tgaaagagag
     9541 gtgccgactg tgaaagtcaa taaaatccgt atgcgggaaa cagtgatttc ctacgctttc
     9601 ctagcaccag tattattctt ctttgtcatc tttgtgttgg ctccgatggt gatgggcttc
     9661 attacaagtt tctttaacta ctcaatgact aaatttgagt ttgtgggctt agataactat
     9721 atccgtatgt ttaaagatcc tgtctttaca aaatctctga ttaacacagt tattttggtt
     9781 attggatctg taccagttgt tgttctattc tcactctttg tagcatctca gacctatcat
     9841 caaaatgtca ttgccagatc cttctaccgt ttcgtcttct tccttcctgt tgtaacgggt
     9901 agtgttgccg tgacagttgt ttggaaatgg atttatgacc cactatcagg gattctaaac
     9961 tttgtcctta agtcaagcca catcatcagc caaaacattt cttggttggg agataaaaac
    10021 tgggcattga tggcgattat gattattctc ttgaccactt cagttggtca gcccatcatc
    10081 ctttatatcg ctgccatggg gaatattgac aattcactgg ttgaagcggc gcgtgttgat
    10141 ggtgcaactg agtttcaagt tttttggaag attaaatggc caagccttct tccaacaact
    10201 ctttatattg caatcatcac aacaattaac tcattccagt gtttcgcctt gattcagctt
    10261 ttgacatctg gtggtccaaa ctactcaaca agtaccttga tgtactacct ttacgaaaaa
    10321 gccttccaat tgacagaata cggctatgcc aacacaattg gtgtcttctt ggcagtcatg
    10381 attgctatcg taagctttgt tcaatttaaa gtacttggaa acgacgtaga atactaaaga
    10441 aaggagacag ctatgcaatc tacagaaaaa aaaccattaa cagcctttac tgttatttca
    10501 acaatcattt tgctcttgtt gactgtgctg ttcatctttc cattctactg gattttgaca
    10561 ggggcattca aatcacaacc tgatacaatt gttattcctc ctcagtggtt ccctaaaatg
    10621 ccaaccatgg aaaacttcca acaactcatg gtgcagaacc ctgccttgca atggatgtgg
    10681 aactcagtat ttatctcatt ggtaaccatg ttcttagttt gtgcaacctc atctctagca
    10741 ggttatgtat tggctaaaaa acgtttctat ggtcaacgca ttctatttgc tatctttatc
    10801 gctgctatgg cgcttccaaa acaagttgtc cttgtaccat tggtacgtat cgtcaacttc
    10861 atgggaatcc acgatactct ctgggcagtt atcttgcctt tgattggatg gccattcggt
    10921 gtcttcctca tgaaacaatt tagtgaaaat attcctacag agttgcttga atcagctaaa
    10981 atcgacggtt gtggtgagat tcgtaccttc tggagtgtag ccttcccgat tgtgaaacca
    11041 gggtttgcag cccttgcaat ctttaccttc atcaatactt ggaatgacta cttcatgcaa
    11101 ttggtaatgt tgacttcacg taacaatttg accatctcac ttggggttgc gaccatgcag
    11161 gctgaaatgg caaccaacta tggtttgatt atggcaggag ctgcccttgc tgctgttcca
    11221 atcgtcacag tcttcctagt cttccaaaaa tccttcacac agggtattac tatgggagcg
    11281 gtcaaaggat aatactctga gaaaatctct tcaaactacg tcagcgtcac cttgccatac
    11341 ttaagtattg cctgcggtta gcttcctagt ttgttcttca attttcattg agtataggaa
    11401 aatcaatcta tcaagataca gaagtatatt ttatagattt agagaatata gaggttataa
    11461 gtgtctacaa aatggagggt atgcagttac tttatgaagt tttgtcagac acttataaac
    11521 ttaagaatgg ttttagttaa ctatcagaaa cgaaggaaag agtatgattt ttgacgattt
    11581 gaaaaacatc accttttaca aagggattca tcccaattta gacaaggcta tcgactatct
    11641 ctaccaacat cgtaaagatt cattcgaatt aggaaagtat gagattgatg gagataaagt
    11701 ctttctagtt gttcaggaaa atgtcctcaa tcaagttgag aataatcaat ttgaacacca
    11761 taagaactat gcagatttgc atttgctggt agaaggacat gaatattcga gctacggttc
    11821 acgtatcaaa gacgaggcag tagcattcga cgaagcgact gacattggct ttgttcattg
    11881 tcatgaacac tacccactct tgttgggtta tcataatttt gcgattttct tcccaggtga
    11941 gccacatcag ccaactggct atgcaggtat ggaagaaaag gttcgaaaat atctctttaa
    12001 aattttgatt gattaaaaat aggatgaatt gtttttttgt aaagctttga caatactcta
    12061 ccatgaaatt gatctttgtg aggtagagaa atgagaataa aatatttaaa aattggtatc
    12121 ttctaagcat gctgcaagag ctagtttctt agatggacag gggattacag ttgatgagat
    12181 gtcctggata attaatggca ttgtgaatgc attgattggt agatacataa aattaggtac
    12241 ttatgcagct aagtatggta ttagtatagc atgctcgatc ttaagtaggg cagctgcaac
    12301 tgcagcagca agagtaggat tactgaccaa gatttctgga tggattttac gagtagctgt
    12361 gaatgtagct gatgtatatg gtaattttgc caacaatatt gctgcagctt gggatgcata
    12421 tgataaaatt cctaacaatg gtcgtataaa cttttaaaat gcgagaatga aagcactttg
    12481 tattttttta ttgaatatgt tagcttggac agtgcttgca atgataattc gtggagggct
    12541 agatggattt gataggcata cttggagtac tattttaatt gcttcgctgt tcggggtata
    12601 tgattataag cccatagata aaaatagaaa aaagtccaaa agaaaaaaga aaaaatagat
    12661 ttgttcatgg tagggactta tgaaagcttt actgacaaaa aagaaaacag tttacaaaga
    12721 aaaatgatgg aggagcaaac atggcacaaa aaggagtaag ccttatcaag gcagcatttg
    12781 atacagataa ctttctcatg cgttttagtg agaaggtctt ggacatcgtg acagccaatc
    12841 ttctttttgt cgtctcttgt ttgcccatcg tgacgattgg agtggctaaa atcagcctct
    12901 acgagaccat gttcgaagtt aagaagagca gacgggtgcc tgtttttaaa atctatctaa
    12961 gatctttcaa gcaaaatctg aaactaggtc ttcagttagg tctgcttgag ttgggcattg
    13021 tttcattaag cctcctagat ctttatctct tttggggtca aacagctctg ccctttcaat
    13081 tgctgaaagc tatttgtcta ggcattctga ttttccttac gattgtgatg ttggctagct
    13141 atccaatcgc ggcacgttat gatttaactt ggaaagaaat tcttcaaaaa ggattgatgt
    13201 tggctagttt taactttcct tggttcttcc tcatgttagc cattcttgtc ctcattgtga
    13261 tggttcttta tctgtctgcc ttcagtctac tcttaggtgg ctcagtcttc ctgctttttg
    13321 ggtttggact attggtcttt atccagactg gattgatgga gaaaattttc gcaaaatacc
    13381 aataggagcc ttatttctga aactactttc aaaggctcca aacgctattc tataagcgag
    13441 aaactaaaat ctaagtgtct ataagatatt gaaagccttt tcggtaaagt gtatactaaa
    13501 gaagtaaaga aaaatctgct ttagcagaaa ataaaaataa taggagaaaa tctatgtcag
    13561 atttgaaaaa atacgaaggt gtcattccag ccttctacgc atgttatgat gagcaaggag
    13621 aagtaagtcc agagcgtacg cgtgccttgg ttcaatactt cattgataaa ggtgttcaag
    13681 gtctttatgt caatggttct tctggtgaat gtatctacca aagcgttgaa gatcgcaagt
    13741 tgattttgga agaagtcatg gcggtagcca aaggtaaatt gaccattatt gcccatgttg
    13801 cttgcaataa tactaaagat agtatggaac ttgctcgcca tgctgaaagc ttgggagtag
    13861 atgctattgc aacgattcca ccaatttact tccgcttgcc agaatactca gttgccaaat
    13921 actggaacga tattagttct gcagctccaa acacagacta cgtgatttac aacattcctc
    13981 aattggcagg ggttgctttg actccaagcc tttacacaga aatgttgaaa aatcctcgtg
    14041 ttatcggtgt taagaactct tctatgccag ttcaagatat ccaaaccttt gtcagccttg
    14101 gcggggaaga ccatattgtc tttaacggtc cagatgaaca gttcctaggt ggccgcctca
    14161 tgggtgctaa ggctggtatc ggtggtactt atggtgctat gccagaactc ttcttgaaac
    14221 tcaatcagtt gattgcggat aaggacctag aaacagcgcg tgaattgcag tatgctatca
    14281 acgcaatcat tggtaaactc acttctgctc atggaaatat gtacggtgtc atcaaagaag
    14341 tcttgaaaat caatgaaggc ttgaatattg gatctgttcg ttcaccattg acaccagtaa
    14401 ctgaagaaga tcgtccagtt gtagaagcgg ctgcagcctt gattcgtgaa accaaggagc
    14461 gcttcctcta atctaaaagg aggtatttat gacatactac gttgcaattg atatcggtgg
    14521 aactaacatc aagtatggtt tggttgatca agaggggcaa cttcttgaat cgcatgaaat
    14581 gccaactgag gcgcataagg gtggacctca tatcttacaa aagaccaaag atatcgtagc
    14641 tagttattta gaaaaaggcc cagtagcagg tgttgccata tcttctgctg ggatggtgga
    14701 tccggataag ggtgagattt tctatgctgg gccgcaaatc cctaactacg caggcaccca
    14761 gttcaaaaag gaaatcgaag aaagctttac tattccttgt gagattgaaa atgatgtcaa
    14821 ctgtgcaggt cttgctgagg cagtatctgg ttcaggcaag ggagcaagtg tgacactttg
    14881 cttgaccatt ggaacaggta tcggtggttg cttgattatg gataggaaag tcttccatgg
    14941 ttttagtaat tcagcctgtg aagtcgggta tatgcatatg caggatggag cttttcaaga
    15001 cttggcttct acaacagctt tagtgaaata tgtagctgaa gcccatggag aagatgttga
    15061 tcagtggaat ggccgacgta tctttaagga agctactgaa ggaaacaaaa tctgcatgga
    15121 aggtattgac cgtatggttg actatctagg aaaaggtctg gcaaatattt gctacgttgc
    15181 caatccagaa gtggttattc ttggtggtgg tatcatgggg caagaggcta tcctcaaacc
    15241 taagatccgc acagccttga aagaggcttt ggtaccaagt ttagcagaaa aaacacgatt
    15301 agaatttgcc catcaccaaa atacagcagg gatgttgggt gcatattatc attttaagac
    15361 aaaacaatcc tagtttggct cagccaaact aggattttct tacacgtttt tgtctacgat
    15421 agccgttgag ttttttattt tcccagtagc tattaaagat tttttccttg ctttcgcgat
    15481 tgatttccaa aaagtaggca taaatcaaat cgataaagaa gagcatagga agttgagcgg
    15541 atattcgttg gatataggag ggttggctgt gggtggctac aagaacagtc tctgtatagg
    15601 tctggctatc tttattggga acacttgtaa agagtacagt ctttgccccc atctccttgg
    15661 catctaatag actatctaaa atagaaggag ttgagcctga aagtgagaag cccagtacta
    15721 gacaattttc atccatgatg ctggttgtcc aggcaaagcc gtcttggtct gtcaaagctt
    15781 cgcagaccac acctagtcgc ataaaacgta atttcatttc acgggcgacg aggccagaac
    15841 tccctgttcc gaagaagtag atacgctcag catcttcgat tagctgggca attcgttcta
    15901 gttggatttc gtcaatcaag tcttgtgttt gttccctcat gttgctataa cttctgagga
    15961 ctcgtttggt cagtggactg tgcttggaga cttggttggc ttgattttct gcctgatgtt
    16021 ggtattggaa aataaattct cggtagccag taaagccaca ctttttagca aagcgggtca
    16081 aagcagcttg agaaatatgt aatttttggg tgacttgttg agaagataaa tcatctgtaa
    16141 tcgtttcagc ttgcaaaaaa tagcgagcga tttcttgttc taggtctgtc atttcttcaa
    16201 aatgtgaatc aatgatagtt gcgatatctg gtttgtccat agggaaagct cctttacatg
    16261 agtcatactg gaagactaga tcagggaata gtcacacttc attataacac ataatataag
    16321 gatagataaa taaaaacgca tctctgtttt aaaaacgaaa aaatcgaaaa agcttctctc
    16381 ttttccataa ttttctactc aaattgtggt acaattaaga gtaagatttt aagttagaaa
    16441 tgagactgat ttgtatgaga aaatttaaca gccattcgat tccgattcgg cttaatttat
    16501 tgttttcaat cgtcatttta ctctttatga ccattattgg tcgtttgttg tatatgcagg
    16561 ttttgaacaa ggatttttac gaaaaaaagc tagcctcagc tagtcagacc aagattacaa
    16621 gcagttcagc ccgtggggaa atttatgatg ctagtggaaa acctttggta gaaaatacgt
    16681 taaagcaggt tgtttccttt acgcgtagca ataaaatgac agctacagac ttaaaagaaa
    16741 cagctaaaaa gttactgact tatgtgagca tcagttctcc aaatttgaca gaacgccagc
    16801 tggcggatta ctatttggct gatcctgaaa tctataaaaa aacagtggaa gctctcccaa
    16861 gcgagaaacg cttggattca gatggcaatc gtctatccga atcagaactg tataacaatg
    16921 cggtcgatag tgtacaaacg agtcaactaa actatacaga ggatgaaaag aaagaaatct
    16981 atctttttag tcagttaaat gctgttggaa actttgcgac aggaaccatt gcgacagatc
    17041 ctctaaatga ttctcaggtg gctgttattg cctctatttc aaaggagatg cctggcatta
    17101 gtatttctac ttcttgggat agaaaggttt tggaaacttc cctttcttct atagttggga
    17161 gtgtatccag tgaaaaagct ggtctcccag cggaagaagc agaagcctat cttaaaaaag
    17221 gctattctct aaatgatcgt gtaggaacct cctatttgga aaagcaatat gaagagacct
    17281 tacaaggaaa acgctcggta aaagaaatcc atctggataa atatggcaat atggaaagcg
    17341 tggatacaat tgaggaaggt agtaagggaa acaatatcaa actgaccatt gatttggctt
    17401 tccaagatag cgtggatgct ttactgaaaa gttatttcaa ttccgagcta gaaaatggtg
    17461 gagccaagta ttctgaaggt gtctatgcag tcgcccttaa cccaaaaaca ggtgcggttt
    17521 tgtctatgtc agggattaaa catgacttga aaacgggaga gttgacgcct gattccttgg
    17581 gaacggtaac caatgtcttt gttccaggtt cggttgtcaa ggcggcgacc atcagctcag
    17641 gttgggaaaa tggagtcttg tcaggaaacc agaccttgac agaccagtcc attgtcttcc
    17701 aaggttcagc tcccatcaat tcttggtata ctcaggctta cggttcattc cctatcacag
    17761 cggtccaagc tctggagtat tcatcaaata cctatatggt ccaaacagcc ttaggtctta
    17821 tggggcaaac ctatcaaccc aatatgtttg tcggcaccag caatctagag tctgctatgg
    17881 agaaactgcg ttcaaccttt ggcgaatatg gcttgggtac tgcgacagga attgacctac
    17941 cagatgaatc tactggattt gttcccaaag agtatagctt tgctaattac attactaatg
    18001 cctttgggca gtttgataac tatacgccga tgcagttggc tcagtatgta gcaactattg
    18061 caaataatgg tgttcgtgtg gctcctcgta ttgttgaagg catttatggt aataatgata
    18121 agggaggact gggtgacttg attcagcaac tgcaaccgac agagatgaat aaggtcaata
    18181 tatccgactc cgatatgagc atcttgcacc aaggttttta tcaggttgcc catggtacta
    18241 gtggattgac aactggacgt gccttttcaa atggtgcctt ggtatccatt agcggaaaaa
    18301 caggtacagc cgaaagctat gtggcagatg gtcagcaagc aaccaatacc aatgcggtgg
    18361 cctatgcccc atctgataat ccccaaatcg ctgtcgcagt ggtctttcct cataatacca
    18421 atctaacaaa tggtgtagga ccttccattg cgcgtgacat tatcaatctg tatcaaaaat
    18481 accatccaat gaactagaaa ggaaattatg ctttatccaa cacctattgc caagttgatt
    18541 gacagttatt ctaagttacc aggtatcggg attaagacgg ctacgcgtct ggccttttat
    18601 acgattggga tgtctgctga tgatgtcaat gaatttgcaa aaaatctcct ttctgctaag
    18661 agagaattga catattgttc tatttgtgga cgtttgacag acgacgatcc ttgttctatc
    18721 tgtactgatt cgactcgtga ccagacaaca attttagttc ttgaggatag tagagatgtg
    18781 gcagccatgg aaaatatcca agaataccat ggactctatc atgtcctgca tggcctcatt
    18841 tctcctatga atggtatcag tccggacgat atcaatctta agagccttat gactcgtctt
    18901 atggatagtg aggtttcaga agtgattgtg gcgactaatg ctacagcgga tggtgaagcg
    18961 acctccatgt atctttcacg tttgctcaag ccggctggta tcaaggttac gcgtctagca
    19021 cgaggtctcg ctgtgggagc ggacattgag tatgcggacg aagtgacact cttacgagcg
    19081 attgaaaatc ggacagagtt gtaagtgtag gcaaatttac gaactccatt catttataaa
    19141 aaatcaaaga ggctgaaaat cgttcctatc ggcctctttt tgtatagtgt gatgagtaga
    19201 atcaggttca agttttaaaa aaccaagcaa atatgatata ctaaagagcg agtattctag
    19261 tagaattagg acaaataata tgaaacaaac gattattctt ttatatggtg gacggagtgc
    19321 ggaacgcgaa gtctctgtcc tttcagctga gagtgtcatg cgtgcggtca attacgaccg
    19381 tttcacagtc aagactttct ttatcagtca gtcaggtgac tctatcaaaa cgcaggaatt
    19441 tagtcatgct ccggggcaag aagaccgtct catgaccaat gaaaccattg attgggataa
    19501 gaaagttgca ccaagtgcta tctacgaaga aggtgcagtg gtctttccag tccttcacgg
    19561 gccaatggga gaagatggct ctgttcaagg attcttggaa gttttgaaaa tgccttacgt
    19621 tggttgcaac attttgtcat caagtcttgc catggataaa atcacgacta agcgtgttct
    19681 ggaatctgct ggtattgccc aagttcctta tgtggctatc gttgaaggcg atgatgtgac
    19741 tgctaaaata gctgaagtgg aagaaaaatt ggcttatcca gtcttcacta agccgtcaaa
    19801 catggggtct agtgtcggta tttctaagtc tgaaaaccaa gaagaactcc gtcaagcctt
    19861 aaaacttgcc ttccgatatg acagccgtgt cttggttgag caaggagtga atgcccgtga
    19921 aattgaggtt ggcctcttgg gtaactacga tgtcaagagc acgctacctg gagaagttgt
    19981 caaggacgtt gccttttatg actacgatgc caagtatatt gataacaagg ttactatgga
    20041 tattcctgcc aaaatcagtg atgatgtggt ggctgtcatg cgtcaaaatg cagaaacagc
    20101 cttccgtgcc attggtggcc ttggtctatc tcgttgcgat ttcttctata cagataaggg
    20161 agagattttt ctcaacgagc tcaataccat gccaggtttc acccagtggt ctatgtaccc
    20221 actactttgg gaaaatatgg ggatcagcta cccagaacta atcgagcgtt tggttgacct
    20281 tgccaaggaa agttttgaca agcgcgaagc gcatttgata taaaatgaaa gagagggtag
    20341 aagccagaac catcactgca aggtgactag agttctcgga cttcagccct ttttaaagga
    20401 gtagaaatga aattaacaat ccatgaaatt gcccaagttg ttggagccaa aaatgatatc
    20461 agtatctttg aggacactca gttagaaaaa gctgagtttg atagtcgttt gattggaact
    20521 ggagatttat ttgtgccact taaaggtgcg cgtgatggcc atgactttat tgaaacagcc
    20581 tttgaaaatg gtgcagcagt aaccttgtct gagaaagagg tctcaaatca tccttacatt
    20641 ttagtagatg atgttttgac agcctttcaa tccctagcat cctactatct tgaaaaaacg
    20701 actgttgatg tctttgctgt tacaggttca aatggcaaga caacgactaa ggatatgttg
    20761 gcgcatttac tgtcaacaag atacaagacc tacaaaacac aaggcaatta caataatgag
    20821 attggccttc cttacacagt tcttcatatg cctgaaggaa cagaaaagtt ggttttggag
    20881 atgggacagg atcacttggg agatattcat ctcttgtctg aattggctcg tccaaaaaca
    20941 gccatcgtga ccttggttgg agaagctcat ttggcctttt tcaaagatcg ttctgagatt
    21001 gctaaaggaa aaatgcaaat tgcagacgga atggcttcag gttccttgct tttagcgccg
    21061 gctgacccta tcgtagagga ctatttgcca attgataaaa aggtggttcg ttttggtcaa
    21121 ggagcagagc tggaaattac tgacttggtt gagcgtaagg acagtctgac ctttaaggct
    21181 aatttcttgg aacaagccct tgatttgcca gtaactggca agtacaatgc gacaaatgct
    21241 atgattgcat cctatgttgc cttgcaagaa ggagtttcag aggagcaaat tcgtttggca
    21301 ttccaacatc ttgaattgac gcgtaaccgt accgagtgga agaaagcagc caatggagca
    21361 gatatcctgt cagatgttta caatgccaat ccaactgcta tgaaactgat tttagagact
    21421 ttctctgcca ttccagccaa tgaaggtggc aagaaaattg cagtgttggc ggatatgaag
    21481 gagcttggtg accagtctgt tcaacttcat aatcagatga ttttgagcct ctccccagat
    21541 gtgcttgata tcgttatttt ctatggagaa gatattgctc agttggctca attggccagt
    21601 caaatgtttc caatcggcca cgtttactac ttcaagaaaa cagaagacca ggatcaattt
    21661 gaagacctag tcaagcaggt taaggaaagc cttggagcct atgaccaaat cttgctaaaa
    21721 ggttctaact ctatgaatct agccaaattg gtagaaagtt tagaaaatga atgcaagtga
    21781 ttttgctaag tatcttcaaa gaatgattgc tcttacagat actggactaa cctttacaaa
    21841 ggatccgttt gaccgtgagc gctacgaaga cttgcgaagt ctgttatctg aaatgttgaa
    21901 tcaggtatca gaccttgatg cagacgaagt ggcagaactc ttgaaaccga cctccgctta
    21961 tgcgactcct ttaatggacg tccgtgcttg gatagttgag gatgaaaaaa tttgtctggt
    22021 taggggacaa ggagagaatg attgggcttt gccaggtggc tttggcgagg tcggttattc
    22081 accaactgaa aatattctca aggaaattga agaagaaacc ggttttaaag ccaaagttga
    22141 acgactacta gctgtttttg ataccaatcg tttccaacta cagagcaaac aatatgcaaa
    22201 gtttgttttt gaatgtaagc ttctcgatgg acaatttcaa gaaaatcaag aaatcgctga
    22261 ccttcaattt tttgcaattg accaactgcc gaatttatct gaaaaacgca ttaccaagga
    22321 gcaaatagag cttctttggc aggtttatca aggtcatagg gggcaatatc ttgactaaga
    22381 agatgattat cgtatttcta aatccatttt taacaactag catggtataa taatatgcag
    22441 gaaaattttg aattatgagg aagactagat gaatttatgg gatattttct ttacgactca
    22501 ggcaaccgag ccgcccaaat ttgacctttt ttggtatgtt agcctattta cgctcttagc
    22561 cttaaccttt tatacagccc atcgctatcg tgaaaataag gtttaccaac gatttttcca
    22621 aatcttgcag actgttcagt taatccttct ttatggttgg tactgggtca atcatatgcc
    22681 actgtcagaa agcctaccct tttaccattg ccgtatggct atgtttgtgg tactcttgct
    22741 tcctggtcaa tccaaatata aacaatactt tgcattattg ggaacatttg ggacattagc
    22801 agcctttgtt tatccagtgc cagatgctta cccttttcca catatcacca ttctatcctt
    22861 tatctttggt catttagcac tcttggggaa ctctctagtt tatctattga gacagtataa
    22921 tgcgcgattg ctggatgtga agggaatttt tctcatgacc tttgccctaa atgccttgat
    22981 ttttgtggtc aatttggtga caggtggcga ttacggattt ttgacaaaac cgccattggt
    23041 tggggatcac ggtctagtag ctaattattt acttgtttca attgtgctgg tagctactat
    23101 cagtttgact aagaaaatct tagaattctt tttagctcaa gaagcagaaa aaatgattgc
    23161 aaaggaagct taacacagag ctttcttttt tgctcttaga gagtttttac aagcagctta
    23221 taaaataaga atttctgaat agacaaactc aaaaaatggc tgggaaattt aggaaaaaag
    23281 caagcacgat taaatttttt gtgttataat attttgtgaa tagctatgcc tatgtttagc
    23341 tatggaataa tacgaagtgc gaaacttgga agatagagag gaagcgatgt aatggctaga
    23401 gaaggctttt ttacaggtct agatattgga acaagctctg tcaaggtgct tgtggccgag
    23461 cagagaaatg gtgaattaaa tgtaattggc gtgagtaatg ccaaaagtaa aggtgtaaag
    23521 gatggaatta ttgttgatat tgatgcagca gcaactgcta tcaagtcagc catttcccaa
    23581 gcggaagaaa aggcaggcat ttcgattaaa tcagtgaatg tcggcttgcc tggtaatctt
    23641 ttgcaggtag aaccaactca ggggatgatt ccagtaacat ctgatactaa ggaaattacg
    23701 gatcaagatg ttgaaaatgt tgtcaaatca gctttgacaa agagtatgac acctgaccgt
    23761 gaagtcatta cctttattcc tgaagaattt attgtggatg gtttccaagg gattcgtgac
    23821 ccacgtggca tgatgggggt tcgccttgaa atgcgtggtt tgctttatac agggcctcgt
    23881 actatcttgc acaatttgcg taagacggtt gagcgtgcag gtgttcaggt tgaaaatgtt
    23941 atcatttcac cactagcaat ggttcagtct gttttgaacg aaggggaacg tgaatttggt
    24001 gctacagtga ttgatatggg ggcaggtcaa acgactgtcg ctacaatccg taatcaagaa
    24061 ctccagttca cacatattct ccaagaaggt ggagattatg taactaaaga tatctccaag
    24121 gttttgaaaa cctctcgcaa attagcggaa ggcttgaaac tgaattacgg ggaagcctat
    24181 ccgcctcttg caagcaaaga aaccttccaa gtagaggtta ttggagaagt agaagcagtc
    24241 gaagtgacgg aagcctactt gtcagaaatt atttctgcac gaatcaagca catccttgaa
    24301 caaatcaagc aagaattaga tagaaggcgt ctattggacc tccctggtgg tattgtctta
    24361 atcggtggga atgccatttt accaggtatg gttgagcttg ctcaggaagt ctttggcgtc
    24421 cgtgtcaagc tttatgtccc aaatcaagtt ggtatccgta atccagcctt tgcgcatgtg
    24481 attagtttat cagaatttgc gggtcaatta acagaagtta atcttttggc tcagggagcg
    24541 ataaaaggtg agaatgactt aagtcatcag ccaattagtt ttggtgggat gctgcaaaaa
    24601 acagctcagt ttgtacaatc aacgcctgtt caaccagctc ctgctccaga agtagagccg
    24661 gtggcgccta cagaaccaat ggcggatttc caacaagctt cacaaaataa accgaaatta
    24721 gcagatcgtt tccgtggctt gatcggaagc atgtttgacg aataaagagg aaaaataaat
    24781 tatgacattt tcatttgata cagctgctgc tcaaggggca gtgattaaag taattggtgt
    24841 cggtggaggt ggtggcaatg ccatcaaccg tatggtcgac gaaggtgtta caggcgtaga
    24901 atttatcgca gcaaacacag atgtacaagc attgagtagt acaaaagctg agactgttat
    24961 tcagttggga cctaaattga ctcgtggttt gggtgcagga ggtcaacctg aggttggtcg
    25021 taaagccgct gaagaaagcg aagaaacact gacggaagct attagtggtg ccgatatggt
    25081 cttcatcact gctggtatgg gaggaggctc tggaactgga gctgctcctg ttattgctcg
    25141 tatcgccaaa gatttaggtg cgcttacagt tggtgttgta acacgtccct ttggttttga
    25201 aggaagtaag cgtggacaat ttgctgtaga aggaatcaat caacttcgtg agcatgtaga
    25261 cactctattg attatctcaa acaacaattt gcttgaaatt gttgataaga aaacaccgct
    25321 tttggaggct cttagcgaag cggataacgt tcttcgtcaa ggtgttcaag ggattaccga
    25381 tttgattacc aatccaggat tgattaacct tgactttgcc gatgtgaaaa cggtaatggc
    25441 aaacaaaggg aatgctctta tgggtattgg tatcggtagt ggagaagaac gtgtggtaga
    25501 agcggcacgt aaggcaatct attcaccact tcttgaaaca actattgacg gtgctgagga
    25561 tgttatcgtc aacgttactg gtggtcttga cttaaccttg attgaggcag aagaggcttc
    25621 acaaattgtg aaccaggcag caggtcaagg agtgaacatc tggctcggta cttcaattga
    25681 tgaaagtatg cgtgatgaaa ttcgtgtaac agttgttgca acgggtgttc gtcaagaccg
    25741 cgtagaaaag gttgtggctc cacaagctag aactgctact aactaccgtg agacagtgaa
    25801 accagctcat tcacatggct ttgatcgtca ttttgatatg gcagaaacag ttgaattgcc
    25861 aaaacaaaat ccacgtcgtt tggaaccaac tcaggcatct gcttttggtg attgggatct
    25921 tcgccgtgaa tcgattgttc gtacaacaga ttcagtcgtt tctccagtcg agcgctttga
    25981 agccccaatt tcacaagatg aagatgaatt ggatacacct ccatttttca aaaatcgtta
    26041 agtaaatgaa tgtaaaagaa aatacagaac ttgtttttcg agaagttgca gaggctagtc
    26101 tgagtgctaa tcgagagagt ggttcggtct ctgtcattgc agttaccaag tatgtagatg
    26161 taccgacagc ggaagccttg cttccgctag gtgttcatca tatcggtgaa aatcgtgtag
    26221 ataagtttct ggaaaaatat gaagctttaa aagatcgaga tgtgacttgg catttgattg
    26281 gtaccttgca aagacgtaag gtgaaagatg tcattcaata cgttgattat ttccatgcat
    26341 tggactcagt aaagctagca ggggaaattc aaaaaagaag tgaccgagtc atcaagtgtt
    26401 tccttcaagt aaatatttct aaagaagaaa gcaaacacgg tttttcgaga gaggaactgc
    26461 tggaaatctt gccagagtta gccagactag ataagattga atatgttggt ttaatgacga
    26521 tggcaccttt tgaggctagc agtgagcagt tgaaagagat tttcaaggcg acccaagatt
    26581 tacaaagaga aattcaagag aaacaaattc caaatatgcc tatgaccgag ttaagtatgg
    26641 gaatgagtcg tgattataaa gaagcgattc aattcggttc cacttttgtt cgtataggta
    26701 catcattttt taagtaggag agaaccatgt ctttaaaaga tagattcgat agatttatag
    26761 attattttac ggaggatgag gattcaagtc tcccttatga aaaaagagat gagcctgtgt
    26821 ttactccagt aaattcttca caggaaccgg ctctcccaat gaatcaacct tcacagtcgg
    26881 ctggtacaaa agagaacaat atcaccagac ttcatgcaag acaacaggaa ttggcaaatc
    26941 agagtcagcg tgcaacggat aaggtcatta tagatgttcg ttatcctaga aaatatgagg
    27001 atgcaacaga aattgttgat ttattggcag gaaacgaaag tatcttgatt gattttcagt
    27061 atatgacaga ggtgcaggct cgtcgttgtt tggactattt ggatggagct tgtcatgttt
    27121 tagctggaaa tttgaaaaag gtagcttcta ccatgtattt gttgacacca gtgaatgtta
    27181 ttgtaaatgt tgaagatatc cgtttaccag atgaagatca acagggtgag ttcggttttg
    27241 atatgaagcg aaatagagta cgataatgat ttttttaatt cgtatgattt ataatgcagt
    27301 ggatatttac tccctgattt tggtagcctt cgctgtcatg tcttggtttc caggtgccta
    27361 cgaatccagt ttaggtcgtt ggattgtagc gttggtgaaa ccagtacttg ctcccttgca
    27421 acgcctgcct ttacagatag cgggtcttga tttatctgtt tgggtcgcga ttgttttggt
    27481 tcgattttta ggagaaaacc tagtgcgttt tctggcgatg ataggatgaa taaagggatt
    27541 tatcagcatt tctccataga agatcgtcca tttcttgaca agggaatgga atggataaag
    27601 aaggtagaag atagctatgc tcctttttta actcctttta tcaatcctca tcaggagaag
    27661 ctattaaaga ttttggccaa aacctatggt cttgcttgta gcagtagtgg ggaattcgtc
    27721 tcgagtgagt atgttcgagt tttattatac ccagattatt tccaaccaga gttttcagat
    27781 tttgaaatat ctctccaaga aattgtgtat tccaataaat ttgaacattt aacgcatgct
    27841 aagattttag ggacagtcat caatcaatta gggattgaac ggaaactttt tggagatatc
    27901 ctagtagatg aagaacgggc gcagattatg attaatcagc agtttcttct tctctttcaa
    27961 gatggactaa agaaaattgg tcgtatacct gtttcgctgg aggaacgtcc tttcaccgag
    28021 aaaatagata agctagaaca gtatcgagaa ctgaatttat ctgtgtctag ttttcgatta
    28081 gatgttcttt tatcaaatgt tttgaaacta tctaggaatc aagcaaacca gttgattgaa
    28141 aagaaacttg tccaagtaaa ttatcatgtg gtagacaaat cagattacac tgttcaagtt
    28201 ggagacttga ttagtgtgag aaaatttggt cgcttgagat tatttcaaga taagggacaa
    28261 acgaaaaaag agaagaaaaa aataaccgtc cagttattat taagtaagtg aggaatagaa
    28321 tgccaattac atcattagaa ataaaggaca agacctttgg aactcgattc agaggttttg
    28381 atccagaaga agtcgatgaa tttttagata ttgtggttcg tgattacgaa gatcttgtgc
    28441 gtgcgaatca tgataaaaat ttgcgtatta agagtttaga agagcgtttg tcttactttg
    28501 atgaaataaa agattcattg agccagtctg tattgattgc ccaggataca gctgagagag
    28561 tgaaacaggc ggcgcatgaa cgttcaaaca atatcattca tcaagcagag caagatgcgc
    28621 aacgcttgtt ggaagaagct aaatataagg caaacgagat tcttcgtcaa gcaactgata
    28681 atgctaagaa agtcgctgtt gaaacagaag aattgaagaa caagagccgt gtcttccacc
    28741 aacgtctcaa atctacaatt gagagtcagt tggctattgt tgaatcttca gattgggaag
    28801 atattctccg tccaacagct acttatcttc aaactagtga tgaagccttt aaagaagtgg
    28861 ttagcgaagt acttggagaa ccgattccag ctccaattga agaagaacca attgatatga
    28921 cacgtcagtt ctctcaagca gaaatggaag aattacaagc tcgtattgag gtagccgata
    28981 aagaattgtc tgaatttgaa gctcagatta aacaggaagt ggaaactcca actcctgtag
    29041 tgagtcctca agttgaagaa gagcctctgc tcatccagtt ggcccaatgt atgaagaacc
    29101 agaagtagct ccagtgcatc cgacaggtcc aacaccagct acagaaactg ttgattcagc
    29161 accaggattt gaagcaccgc aagaatccgt tacaatttta taagaaacga tgtgagaaca
    29221 atatcttatc cttatatttc cagcgagcag gagatggtgt gagtcctgca ttccctaacg
    29281 ataagattat cctctcaaaa actcaagtct gaagctagta agatttgacg tttcccacgt
    29341 tacgggataa gagggagaaa gactaaatct ttttccgaat aaaggtggta ccacgatttt
    29401 cgtccttttt ggaagtcgtg gtttttaatt tgttattatt tataaaggag ataccatgaa
    29461 actcaaagat acccttaatc ttgggaaaac agctttccca atgcgtgcag gccttccgac
    29521 caaagagcca gtttggcaaa aggaatggga agatgcaaaa ctttaccaac gtcgtcaaga
    29581 actaaatgaa gggaaaccgc attttgtctt gcatgatggc cctccatacg caaacggaaa
    29641 tatccacgtt ggacatgcca tgaatcatat ctcaaaagat atcatcattc gttctaagtc
    29701 tatgtcaggt tttaacgcac cttatatccc aggttgggat actcatggtc tgccaatcga
    29761 gcaagttttg gcaaagcaag gtgtgaagcg caaagaaatg gacttggttg agtacttgaa
    29821 actctgtcgt gagtacgctc tttctcaagt ttataaacaa cgtgatgatt tcaaacgctt
    29881 ggggatgtca ggtgactggg aaaatttata tgtgacttta actccagact atgaagcagc
    29941 ccaaatccgt gtctttggtg agatggctaa taagggttat atctaccgtg gtgccaagcc
    30001 agtttactgg tcatggtcat ctgagtcagc ccttgccgaa gcggaaattg aataccatga
    30061 tttggtttcg acttcacttt attatgccaa taaggtaaaa gatggcaaag gtatcctaga
    30121 tacagatact tatatcgtag tctggacaac aactccattt accatcacag cttctcgtgg
    30181 tttgactgtt ggtgcagata ttgattacgt tttggttcaa cctgctagtg aaactcgtaa
    30241 gtttgtcgtt gctgcagaat tgttgactag cttgtctgag aaatttggct gggttgatgt
    30301 tcaagtgttg gcaacttacc gtggtcaaga actcaaccac atcgtaacag aacacccatg
    30361 ggatacagct gtagatgagt tggtaatcct tggtgatcac gttaccactg actctggtac
    30421 aggtatcgtc catacagccc ctggttttgg tgaggacgac tacaatgttg gtattgctaa
    30481 tggtcttgaa gtcgcagtga ctgttgacga acgcggtatc atgatggcga atgctggtcc
    30541 tgagtttgaa ggtcagttct acgacaaggt tgtaccaact gttattgaaa aacttggtaa
    30601 cctccttcta gcccaagaag aaatctctca ctcataccca tttgactggc gtactaagaa
    30661 accaatcatc tggcgtgcag ttccacaatg gtttgcctca gtttcaaaat tccgccaaga
    30721 aatcttggat gctattgata aagttaaatt ccatacagaa tggggtaaag tacgtctata
    30781 taatatgatt cgtgaccgtg gtgactgggt tatctctcgt caacgtgctt ggggtgttcc
    30841 acttccaatc ttctatgcag aagacggtac agctatcatg acagctgaga ctattgaaca
    30901 tgtggcgcaa ctctttgaag tacatggttc aagcatttgg tgggaacgtg atgccaaaga
    30961 cctcttacca gaaggattta cacatccagg ttctccaaat ggggagttca agaaagaaac
    31021 agatattatg gatgtatggt ttgactcagg ttcatcatgg aatggtgttt tggtaaatcg
    31081 tccaaatctg acttatccag ctgaccttta ccttgaaggt tctgaccaat accgtggttg
    31141 gttcaactca tcactcatta catctgttgc caaccatggc gtagcacctt acaaacaaat
    31201 cttgtcacaa ggttttaccc ttgatggtaa aggtgagaag atgtctaaat cacttggaaa
    31261 taccattgct ccaagtgatg ttgaaaaaca atttggtgca gaaatcttgc gtctctgggt
    31321 aacaagtgtt gactcaagca atgacgtgcg tatctctatg gatattttga gccaagtttc
    31381 tgaaacttac cgtaagattc gtaacactct tcgtttcttg attgccaata catctgactt
    31441 taacccagct caagatgtag tcgcttacga tgaacttcgt tcagttgata agtacatgac
    31501 tattcgcttt aaccagcttg tcaagaccat tcgtgatgcc tatgcggact ttgaattctt
    31561 gacaatctac aaggccttgg tgaactttat caacgttgac ttgtcagcct tctaccttga
    31621 ttttgccaaa gatgttgttt acattgaagg tgccaaatca ttggaacgcc gtcaaatgca
    31681 gactgttttc tatgacattc ttgtcaaaat caccaaactc ttgacaccaa tccttcctca
    31741 cactgcggaa gaaatctggt catatcttga gtttgaagct gaagacttcg ttcaattgtc
    31801 agaattgcca gaagctcaaa cttttgctaa tcaagaagaa gtcttggata catgggcagc
    31861 cttcatggac ttccgtggac aagctcaaaa agccttggaa gaagctcgta atgcaaaagt
    31921 tatcgggaaa tcgcttgaag cacacttgac agtttatcca aatgaagttg tgaaaactct
    31981 actcgaagca gtaaacagca atgtggctca acttttgatc gtgtcagact tgaccatcgc
    32041 agaagggcca gctccagaag ctgcccttag cttcgaagat gtagccttca cagttgaacg
    32101 cgctgcaggt caagtatgtg accgttgccg tcgtatcgac ccaacaacag cagaacgcag
    32161 ctaccaggca gtcatctgtg accactgtgc aagcatcgta gaagaaaact ttgcggaagc
    32221 agtcgcagaa ggatttgaag agaaataagg ttgaaaagtc taggcaaaat tcaatttgag
    32281 aagaaaagac aactaatttt atagtctatt aaacgcattg tatcacgttt ttgaatacct
    32341 gatatgatgc gttttttatt tattttaaaa atttgcgagg tatgactttt tatactcaac
    32401 aagaatcaaa gagaaactta gcaagctaac agtagtaaga taaaatagga
//
LOCUS       DAVKHZ010000014        32085 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000014 DAVKHZ010000000
VERSION     DAVKHZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.014"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(543..635)
                     /locus_tag="VWW99_000628"
     CDS             complement(543..635)
                     /locus_tag="VWW99_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9538508.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            796..1767
                     /gene="ruvB"
                     /locus_tag="VWW99_000629"
     CDS             796..1767
                     /gene="ruvB"
                     /locus_tag="VWW99_000629"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9538509.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1856..2320
                     /locus_tag="VWW99_000630"
     CDS             1856..2320
                     /locus_tag="VWW99_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9538510.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQFYMHQHSPHTAFYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2551..3309
                     /locus_tag="VWW99_000631"
     CDS             2551..3309
                     /locus_tag="VWW99_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9538511.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3318..4121
                     /locus_tag="VWW99_000632"
     CDS             3318..4121
                     /locus_tag="VWW99_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9538512.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4143..5402
                     /gene="rseP"
                     /locus_tag="VWW99_000633"
     CDS             4143..5402
                     /gene="rseP"
                     /locus_tag="VWW99_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9538513.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5415..7268
                     /locus_tag="VWW99_000634"
     CDS             5415..7268
                     /locus_tag="VWW99_000634"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9538514.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7368..8747
                     /gene="bglA"
                     /locus_tag="VWW99_000635"
     CDS             7368..8747
                     /gene="bglA"
                     /locus_tag="VWW99_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9538515.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            8939..10747
                     /gene="glmS"
                     /locus_tag="VWW99_000636"
     CDS             8939..10747
                     /gene="glmS"
                     /locus_tag="VWW99_000636"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9538516.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVAPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYSMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(10884..10961)
                     /locus_tag="VWW99_000637"
                     /pseudo
     CDS             complement(10884..10961)
                     /locus_tag="VWW99_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11018..12067
                     /locus_tag="VWW99_000638"
     CDS             11018..12067
                     /locus_tag="VWW99_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9538517.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKIGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(12167..15964)
                     /gene="spuA"
                     /locus_tag="VWW99_000639"
     CDS             complement(12167..15964)
                     /gene="spuA"
                     /locus_tag="VWW99_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9538518.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPVTPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16381..16794
                     /gene="rpsL"
                     /locus_tag="VWW99_000640"
     CDS             16381..16794
                     /gene="rpsL"
                     /locus_tag="VWW99_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9538519.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16814..17284
                     /gene="rpsG"
                     /locus_tag="VWW99_000641"
     CDS             16814..17284
                     /gene="rpsG"
                     /locus_tag="VWW99_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9538520.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17709..19790
                     /gene="fusA"
                     /locus_tag="VWW99_000642"
     CDS             17709..19790
                     /gene="fusA"
                     /locus_tag="VWW99_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9538521.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19894..24285
                     /locus_tag="VWW99_000643"
     CDS             19894..24285
                     /locus_tag="VWW99_000643"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9538522.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VDKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLVTGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24387..24650
                     /locus_tag="VWW99_000644"
     CDS             24387..24650
                     /locus_tag="VWW99_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9538523.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24643..24921
                     /locus_tag="VWW99_000645"
     CDS             24643..24921
                     /locus_tag="VWW99_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9538524.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24942..25094
                     /locus_tag="VWW99_000646"
     CDS             24942..25094
                     /locus_tag="VWW99_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538525.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            25116..26357
                     /locus_tag="VWW99_000647"
     CDS             25116..26357
                     /locus_tag="VWW99_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9538526.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRVYEEDPVKAWENHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAINAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIIDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVGGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26367..26597
                     /locus_tag="VWW99_000648"
     CDS             26367..26597
                     /locus_tag="VWW99_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9538527.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            26865..27587
                     /locus_tag="VWW99_000649"
     CDS             26865..27587
                     /locus_tag="VWW99_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9538528.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            27804..29138
                     /gene="pepC"
                     /locus_tag="VWW99_000650"
     CDS             27804..29138
                     /gene="pepC"
                     /locus_tag="VWW99_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9538529.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(29194..30105)
                     /locus_tag="VWW99_000651"
     CDS             complement(29194..30105)
                     /locus_tag="VWW99_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9538530.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(30129..30932)
                     /locus_tag="VWW99_000652"
     CDS             complement(30129..30932)
                     /locus_tag="VWW99_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9538531.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(30960..31949)
                     /gene="manX"
                     /locus_tag="VWW99_000653"
     CDS             complement(30960..31949)
                     /gene="manX"
                     /locus_tag="VWW99_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9538532.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         9127 a         6178 c         7478 g         9302 t
ORIGIN      
        1 taaaatccta aacttttctt tttcataata atctccctta acttcaccca atcaggtgga
       61 gttttttagc tctatttcag gcttttgggg actattctaa aaataatttt tcgatatttt
      121 tcggtatttt tcggattttg gtcggggaat tggcggggac ttttttagcg aatataacta
      181 agaaataggt ctgtggtcgc ttcagctagt tcaaactcaa tttgattgta agtgactgtt
      241 aattgaagaa ctggaactac tgtaataatg attttgcttg tctagtagtg gcgtattaaa
      301 tgagaagtaa catgtaagat tgtctcttcg tttacgaaat caaaatttct atgaaattga
      361 tagaatccct aacgttctct taatcttaac cgtcttgtcg tacagcggtt gaagaaaatg
      421 taaacctttg acaaaattcg tgaactatgg gataataaaa aggaattgag tactagttct
      481 atatcatagg ctagaaaaga ccccaagctc acgaccaaat aagcttgagg tcttttttga
      541 cactatttgt ccttgtttag ccatttatcg actatacgaa gaacgacacc gaccacaatt
      601 ggtccgatga tagttttgag tactaattcc atcatgggct atctcacctc ctttcgtagg
      661 cggtgtagca gtgtcgtaga atattatacc acataagtgc taaacttagg agtcacccaa
      721 tcaggtggct tttttgtttg tggcttggtt ttttgatata atagagccat gagtagaatt
      781 ttagataatg agataatggg ggatgaggag ttagtagaac gcacgctccg tcctcagtat
      841 ttacgtgaat atatcggaca ggataaggtc aaggaccagc tacaaatctt tattgaagct
      901 gccaaaatgc gggatgaagc gctggatcat gtgctcttat ttgggcctcc aggtctcggg
      961 aaaacgacca tggcctttgt tattgccaac gaactgggag tcaatcttaa gcagacgtcg
     1021 ggtccagtca ttgaaaaagc cggagatctg gtagctattt tgaatgagtt agagcctggg
     1081 gatgtacttt ttattgatga gatccatcgt ttgccaatgt cagtggaaga ggtgctttat
     1141 agtgctatgg aggacttcta catcgatatt atgattgggg ctggtgaggg tagtcgtagt
     1201 gttcatttgg agttaccacc ttttaccttg attggtgcga cgactcgggc tggtatgctc
     1261 tccaatccgc tacgggcacg ttttgggatt acaggccata tggagtatta tgcccatgct
     1321 gacttgacag aaattgtcga gcggacggca gatatttttg agatggaaat cactcatgag
     1381 gcagcatctg agttggccct acgtagtcgt gggacccctc gtattgccaa tcgtctcctc
     1441 aagcgcgtgc gcgattttgc ccagataatg gggaatgggg taattgatga tattattacc
     1501 gataaggctt tgactatgct ggatgttgac catgaaggtt tggactatgt ggatcaaaaa
     1561 atccttcgta ccatgattga gatgtacagt ggaggacctg ttggtctagg aactctttct
     1621 gtgaatatcg ccgaagagcg tgagacagtt gaagatatgt atgagcctta cttgattcaa
     1681 aaaggtttta tcatgcggac acggtctgga cgggtggcga ctgctaaggc atatgagcac
     1741 ttaggttatg aatacagtga aaaataagca agaaatctta gaggctttta gagaaaatcc
     1801 agatatgatg gccattctga cgatcatccg agaccttggt ttgaaagact cgtagttggc
     1861 agcaggttct gtcagaaatt tcatctggaa tcttttgtca gacaaatccc cttttgatca
     1921 tgaaacagat atagatgtga ttttctttga tccagatttt tcttatgagg aaaccttatt
     1981 actggagaaa aagctgagag aggattttcc tcagtaccag tgggaattga aaaatcagtt
     2041 ctatatgcat cagcacagtc ctcacactgc tttctatacc agttctcgtg atgctatgag
     2101 taagtatcca gaacggtgta cggcagttgg actgcgcttg aatgaagaat cagattttga
     2161 actctatgca ccttatggtt tggaggatat tttgaatttt caagttcgtc caactcctca
     2221 tttcttagaa aatgaagacc gaatggaact ctatcaaaca cgtttatcca agaaaaattg
     2281 gcaggagaaa tggaaaaatt tgatttttaa aaatacttaa ggaaacttta agctagggag
     2341 tgtacactaa gttcataagt taagaagacc ttaacttaaa ctcctaaaac tttttcataa
     2401 taatctccct ataaaaataa agtcgcccaa tcaggcggct tatttttttg aaaaatgggc
     2461 atggtgcctg agaataaata gcttagtgat agaagaaaat ggggaaatat ggtataatga
     2521 aacgatagat ttttgaatag gaataagatc atgtttggat tttttaagaa agataaggct
     2581 gtggaagtag aggttccgac acaggttcct gctcatatcg gcatcatcat ggatggcaat
     2641 ggccgttggg ctaaaaaacg tatgcaaccg cgagtttttg gacataaggc gggcatggaa
     2701 gcattgcaaa ccgtgaccaa ggcagccaac aaactgggcg tcaaggttat tacggtctat
     2761 gctttttcta cggaaaactg gacccgtcca gatcaggaag tcaagtttat catgaacttg
     2821 ccagtagagt tttatgataa ttatgtcccg gaactacatg cgaataatgt taagattcaa
     2881 atgattgggg agacagaccg cctgcctaag caaacctttg aagctttaac caaggctgag
     2941 gaattgacta agaacaacac aggattgatt cttaattttg ctcttaacta tggtggacgt
     3001 gctgagatta cacaggcgct taagttgatt tcccaggatg ttttagatgc caaaatcaat
     3061 ccaggtgaca tcacagagga attgattggc aactatctct ttacccagca tttgcctaag
     3121 gacttacgag acccagactt gattatccgt actagtggag aattgcgttt gagcaatttc
     3181 cttccatggc agggagccta tagtgagctt tattttacgg acaccttatg gcctgatttt
     3241 gacgaagcgg ccttgcagga agctattctt gcctataatc gtcgtcatcg ccgatttgga
     3301 ggagtttagg aggaaatatg acccaggatt tacagaaaag aaccttgttt gcagggattg
     3361 ccctggctat tttcctacca attttaatga ttgggggcct cttgcttcag atagcaatcg
     3421 gaatcatagc catgctagcc atgcatgaac ttttgaagat gagaggtcta gagaccatga
     3481 cgatggaggg cctcttgacc ctctttgcaa cctttgcatt gaccattccc ttggagaatt
     3541 acctgacttt tttgccagtt gatgggaatg tggttgccta tagtgttttg atttcaatca
     3601 tgttaggaac gaccgttttt agcaagtctt atacgattga ggatgcggtt ttccctcttg
     3661 ctatgagctt ctacgtgggc tttggattta atgctttact agatgctcgt gttgcaggtt
     3721 tggacaaggc tctcttagcc ttgtgtatcg tctgggcgac agacagtggt gcctatcttg
     3781 ttgggatgaa ctatgggaaa cgaaagttag caccaagggt atcgcctaat aaaacccttg
     3841 agggtgcctt gggtggtatt ttaggagcaa ttttagtaac cattatcttt atgatagttg
     3901 acagtacagt tgctcttcca tatggaattt acaagatgtc agtctttgct attttcttta
     3961 gcattgctgg acaatttggt gatttactag aaagttcgat caaacgtcat tttggtgtta
     4021 aggattctgg gaaatttatc cctggacatg gtggtgtttt ggatcgtttc gatagtatgt
     4081 tgcttgtatt tccaatcatg cacttatttg gactctttta atcaaaagac ggaggaaacg
     4141 ctatgctcgg aattttaacc tttattctgg tttttgggat tattgtagtg gtgcacgagt
     4201 tcgggcactt ctactttgcc aagaaatcag ggattttagt acgtgaattt gccatcggta
     4261 tgggacctaa aatctttgct cacattggca aggatggaac ggcctatacc attcgaatct
     4321 tgcctctggg tggctatgtc cgcatggccg gttggggtga tgatacaact gaaatcaaga
     4381 caggaacgcc tgttagtttg acacttgctg atgatggtaa ggttaaacgc atcaatctct
     4441 caggtaaaaa attggatcaa acagccctcc ctatgcaggt gacccagttt gattttgaag
     4501 acaagctctt tatcaaagga ttggttctgg aagaagaaaa aacatttgca gtggatcacg
     4561 atgcaacggt tgtggaagca gatggtactg aggttcggat tgcaccttta gatgttcaat
     4621 atcaaaatgc gactatctgg ggcaaactga ttaccaattt tgcaggtcct atgaacaatt
     4681 ttatcttagg tgtcgttgtt ttttgggttt taatctttat gcagggtggt gtcagagatg
     4741 ttgataccaa tcagttccat atcatgcccc aaggtgcctt ggccaaggta ggagtaccag
     4801 aaacggcaca aattaccaag attggctcac atgaggttag caactgggaa agcttgatcc
     4861 aagctgtgga aacagaaacc aaagataaga cggcaccgac tttggatgtg actatttctg
     4921 aaaaggggag tgacaaacaa gtcactgtta cacccgaaga tagtcaaggt cgttaccttc
     4981 taggtgttca accgggggtt aagtcagatt ttctatccat gtttgtaggt ggttttacaa
     5041 ctgctgctga ctcagctctc cgaattctct cagctctgaa aaatctgatt ttccaaccgg
     5101 atttgaacaa gttgggtgga cctgttgcta tctttaaggc aagtagtgat gctgctaaaa
     5161 atggaattga gaatatcttg tacttcttgg caatgatttc catcaatatt gggattttta
     5221 atcttattcc gattccagcc ttggatggtg gtaagattgt gctcaatatc ctagaagcca
     5281 tccgccgcaa accattgaaa caagaaattg aaacctatgt caccttggcc ggagtggtca
     5341 tcatggttgt cttgatgatt gctgtgactt ggaatgacat tatgcgactc ttttttagat
     5401 aatcgaggaa tattatgaaa caaagtaaaa tgcctatccc aacgcttcgc gaaatgccaa
     5461 gcgatgctca agttatcagc catgctctta tgttgcgtgc tggttatgtt cgccaagttt
     5521 cagcaggtgt ttattcttat ctaccacttg ccaaccgtgt gattgaaaaa gctaaaaaca
     5581 tcatgcgcca agaattcgaa aagattggtg ctgttgagat gttggctcct gcccttctta
     5641 gtgcagaatt gtggcgtgaa tcaggtcgtt acgaaaccta tggtgaagac ctttacaaac
     5701 taaaaaaccg tgaaaaatca gactttatct taggtccaac tcatgaagaa acctttacag
     5761 ctattgtccg tgattctgtt aaatcttaca agcaattgcc actcaacctt tatcaaattc
     5821 agcccaagta tcgtgatgaa aaacgcccac gtaatggact tcttcgtaca cgtgagttta
     5881 tcatgaagga tgcttatagt ttccacgcta actatgatag tttggatagt gtttatgatg
     5941 agtacaaagc agcctatgag cgtattttca ctcgtagtgg tttagacttc aaggctatta
     6001 ttggtgacgg tggagccatg ggtggtaagg atagccaaga atttatggcc attacatctg
     6061 ctcgtacaga ccttgaccgc tgggttgtct tggacaagtc agttgcctca tttgacgaaa
     6121 ttcctgcaga agtgcaagaa gaaatcaagg cagaattgct caaatggata gtctctggtg
     6181 aagataccat tgcttactca agtgagtcta gctatgcagc taacttagaa atggcaacaa
     6241 acgagtacaa accaagcaac cgtgttgtcg ctgaagaaga agttactcgt gttgcaacgc
     6301 cagatgttaa atcaattgat gaagttgcag ccttcctcaa tgttccagaa gaacaaacga
     6361 ttaaaaccct cttctacatt gcagatggcg agcttgttgc agcccttcta gttggaaatg
     6421 accaactcaa cgaagtcaag ttgaaaaatc acttgggagc agatttcttt gacgttgcta
     6481 gcgaagaaga agtggcgaat gttgttcaag caggatttgg ttcacttgga ccagttggtt
     6541 tgccagagaa tattaaaatt attgcagatc gtaaggtgca agatgttcgc aatgcagttg
     6601 tcggtgctaa cgaagatggc taccacttga ctggtgtgaa cccaggccgt gattttactg
     6661 cagaatatgt ggatatccgt gaagttcgtg agggtgaaat ttccccagat ggacaaggtg
     6721 tccttaactt tgcgcgtggt attgagatcg gtcatatttt caaactcgga actcgctatt
     6781 cagcaagcat gggagcagat gtcttggatg aaaatggtcg tgctgtgcca atcatcatgg
     6841 gatgttacgg tatcggtgtc agccgtcttc tttcagcagt gatggagcaa cacgctcgcc
     6901 tctttgttaa caaaacgcca aaaggtgaat accgttacgc ttggggaatc aatttcccta
     6961 aagaattggc accatttgat gtgcatttga ttactgttaa tgtcaaggat gaagaagcgc
     7021 aagccttgac agaaaaactt gaagcaagct tgatgggagc tggttatgaa gtcttgacag
     7081 atgaccgtaa cgaacgtgtc ggtgttaaat ttagcgatag tgacttgatt ggtcttccaa
     7141 tccgtatcac tgttggtaaa aaagcggccg atggcattgt agaagttaag atcaaggcaa
     7201 ctggtgacac cattgaggtt catgcagata acgtgcttga aacgcttgaa atcctaagca
     7261 agaaataaaa actataatca gaagaaaaac aaggaaaaaa tgtaactagt ttttaccttg
     7321 ttttttctgt ataatgggag aaatgagtag ataaagaggt aaatgttatg ctaagatttc
     7381 caaaggattt tgtctgggga tcctctactt ctggaccgca gacagaagga cgtgtagctg
     7441 gtgacggtaa gggagacaat ctctgggatt actggttcca agtggagcca aatcgttact
     7501 ataatgggat tggttcagat aagacatcga ctttttatga aaattgggag cgggatattg
     7561 agcttttgtt agagactggt cacacagtct ttcggacttc tattcagtgg tcacggattt
     7621 ttccacaagg ctgtgggaaa gtcaaccctc aaggtgtgga tttttatcgt aaggtctttg
     7681 aggctattaa ggctaaagga attcgtctgt tagtcaatct ctatcatttt gatttacctt
     7741 ttgcccttca agaggatggt gatggttggg aaaataaggc gacagtctca gcctatgaag
     7801 actatgctcg tttttgtttt gagacttatg gagatttagt ggatcaatgg attaccttta
     7861 acgagcccat cgttcctgta gaatttggtt atttttatga tgcccattat ccacataagg
     7921 tggatgcaga ggcagctgtt aaggtagcct atcatacaca attggccagc agccgggctg
     7981 ttaaggcttg ccatgaactt ttgcctgatt ccaagattgg gattgtcctc aacttgacac
     8041 cggcttatcc acgtagccag catcctgctg atgtcaaggc agctcgtatt gcggcccttt
     8101 ttcaggccca atctttctta gatccatctg tcttggggac ttatccacag gagttggtag
     8161 aaatcttgca tgaacacggt cttttacctg atgctacaga ggaagagttg gaactcattc
     8221 gtgataatac ggtggacttc cttggtgtga actactatca acctttgcgt gttatggctc
     8281 ctcgatttgc taagcatcca gagagtccac tcttaccaga acatttttac gagccttatg
     8341 tgatgcctgg acgtaaaatc aattctcacc gtggttggga gatttatgag caagggattt
     8401 atgacatcgc ccaaaatatc aaggaaaatt atggcaatat tgaatggatg ttgactgaga
     8461 atggtatggg tgttgaaggg gaagaaaaat tccgtcaaga tggaatgatt caagatgatt
     8521 accgtattga ctttgtaaaa ggtcatcttc gtgaacttca ccgtgccatt gaagatggtg
     8581 ccaactgtaa gggctacttg atttggacct ttattgactg ctggtcatgg ctcaacagct
     8641 ataaaaatcg ctatggtttg gtcgaattag acttggaaac gcaagaacgt cgtctgaaga
     8701 aatcaggcca ctggttcaaa aaattaagcg ataataatgg attttaaaaa ggactctgac
     8761 tgattatccc aatcggtcag agttttttgc ttacaggagc ttaatttgaa ctataccaat
     8821 ttttactctt gacaagtgaa agaaacaagt atatactgtt tttgctgatt ctataaaaga
     8881 tgaattagac tataccaatt ttaaggagaa agcacagctt gcctgtgtcg tatatactat
     8941 gtgtggaatt gttggtgttg ttggaaacac aaatgcaact gatattttga ttcaagggct
     9001 tgaaaagctt gaataccgtg gctatgattc tgcgggaatt tttgtcctag atggtgctga
     9061 taaccatttg gtgaaggctg ttggtcgtat tgcagaattg tctgccaaga cagctggtgt
     9121 tgagggaaca actggtatcg gacatactcg ttgggctact cacggaaaac caactgagga
     9181 caatgctcac ccacaccgct ctgagacaga acgttttgtc ttggtgcata atggggtgat
     9241 tgagaactac cttgaaatca aggaagaata ccttgcaggt caccacttca aggggcagac
     9301 agatactgaa atagccgttc atttgattgg aaaatttgcg gaagaagaag ggctctcagt
     9361 tcttgaagcc ttcaaaaaag cccttcacat catccgtggt tcttatgcct ttgccttggt
     9421 tgactcacaa gatcctgaag tcatctacgt agctaaaaat aaatcaccac ttttgattgg
     9481 tcttggagaa ggctataaca tggtctgctc agatgctatg gctatgattc gtgaaaccaa
     9541 ccaatacatg gaaattcatg atcaagagtt ggtaatcgtc aaggctgata gcgtggaagt
     9601 tcaagactat gatggtaaca gtcgtgaacg tgctagctat actgcggaac ttgacttgtc
     9661 agatatcggt aagggaactt atccttacta catgcttaag gaaattgatg agcaaccaac
     9721 tgttatgcgt aaactcattc aagcctacac ggatgatgct ggtcaagtag tggttgctcc
     9781 tgctatcatt aaggctgttc aagacgcaga ccgcatctac atccttgcag ctggaacatc
     9841 ttaccatgca ggatttgctt ctaagaaaat gttggaagaa ttgacagata caccagttga
     9901 acttggaatc tcatctgagt ggggctacag tatgccactt ctcagcaaga aaccactctt
     9961 catctttatc agccaatctg gtgaaacagc ggatagtcgt caagttttgg tcaaggctaa
    10021 tgaaatggga attccaagct taacagtgac aaatgttcca ggttcaaccc tctcacgtga
    10081 agccaactat accatgctcc ttcacgcagg tcctgaaatt gctgtggcat caactaaagc
    10141 ctatacagcg caaatcgcag cccttgcctt ccttgcaaaa gcagtcggag aagcaaatgg
    10201 taatgctaaa gcgcaagtct ttgatctggt tcatgaattg tcaatcgtag ctcagtctat
    10261 tgaatcaact ctttcagaga aagaaaccat tgaagtcaag gttcgtgaac ttcttgaaac
    10321 aactcgtaac gccttttaca tcggacgtgg tcaagattac tacgtagcca tggaagcaag
    10381 tctcaaactc aaagagattt cttatatcca gtgtgaaggc tttgctgcag gagaactcaa
    10441 gcacggaacc attgccttga ttgaagaagg aacgcctgtt ttggctctct tgtcagatcc
    10501 agttcttgcc aaccatactc gtggaaatat ccaagaggtc gcagcccgtg gtgccaaagt
    10561 cctcactatc gcagaagaga atgtagccaa agataccgac gatatcgtcc ttacgaccgt
    10621 acatccatac ctctcaccaa tttcaatggt cgtaccaacg caattagtcg cttactttgc
    10681 aaccctccac cgtggcctcg atgtggacaa accacgtaac cttgccaagt cagtaacggt
    10741 agaataagct aaaaaagtct agttatctag gctttttctt gtgagcaaat tggttgcttg
    10801 tactcaagat tcgtgttata ctcttcgaaa atctcttcaa accacgtcaa cgtcgccttg
    10861 ccgtaggtat ggttactgac ttcgttagtt ctatccacaa cctcaaaaca ctgttttgag
    10921 cagcctgcag ctagcttcct agtttgctct ttgattttca ttgagtatta gaataatttt
    10981 tcaaattgaa ggacagagat agacaaggag aatcacaatg gtagaattgg gaatttcaac
    11041 atttggggaa ataacggagc ttgaagggac tggacaaact tacagtcatg ctgaacgcat
    11101 tcgccagttg gtggcagaga ttgagctggc tgacaagatt ggtttggacg tatatgggat
    11161 tggcgagcac catcgggcgg attttgcagt atcagcccca gagattgtcc tggccgctgg
    11221 ggcagtcaat accaagaaaa tccgtttgac cagtgcagtc agcattctgt caagcatgga
    11281 tccgattcgt ttgttccaac agtatgccac tatcgatgct ttgtcaaatg gacgatcgga
    11341 gattatggct ggaaggggct ctttcacgga atctttccct ctgtttggtt atgacttgaa
    11401 agactacgac tctctttttg atgagaaatt agacttgctt cagttagtca atgaaaagac
    11461 caagttagac tggcaaggtc gattgactca aacgattgct ggtaaagaag tttatcctcg
    11521 tccagttcag gacaaattgc ccttgtggat agctacaggt ggtcatgtcg aatcaacagt
    11581 gaagattgct caggctggtc taccgattgt atatgctatt attggtggta atccacgtta
    11641 ttttaaaaag ttgattcagg cttatcgtga gattggaagc gaagcgggcc atgctgacaa
    11701 agacttgaaa gtgggagccc attcttgggg gtggattgcg gaagatggtg agcaggcggt
    11761 gaaagattat ttccatccga ctaagcaagt ggtggatgct atttccaaag accgtccgca
    11821 ctggcaggaa ttgcgttatg agcaatattt ggagcaggta gggccaaatg gcgccatgtt
    11881 tgttggcaat ccagatcagg tggccgaaaa attgattcgc atgatagagg atttaggatt
    11941 ggaccgcttc atgcttcatc taccgcttgg ttctatgcct catgaccagg ttctgagagc
    12001 tattgaactc ttcggaacac aagttgcacc caaagtacga gcttattttg ctatgaaaga
    12061 ggcttaataa aaaatcctaa tcaagtttat taggacaaca aatattgtac gaaaattaat
    12121 cccccgagct ataatgcttg ggggattttt ctataggagg gctagtttag ttctcttttt
    12181 tattttttag taagaaaccg agaccaagga gcgcaaggag gctgattcct gcaaatagga
    12241 gtttgttttc gtttttgatt cctgtatttg gaagttcagc ttgtttatct ggggttacag
    12301 gtatattttc cttgctagag ttttctaccg attcgtttcg aaccgcttct tttacagatg
    12361 atgcttgtgc ttcttgtgct ggttgttcag cttgtgaatc agctgtagct tggctaggtt
    12421 tattttccgc atcagctact ttggcatctg gtttagtaga atcaggtcta gcttctggag
    12481 ctgggtcttg atgtgcaggg tgagaagctt cattttgatg ttcaggcttg gaaggggttg
    12541 agtctggttt ctcttctgca gttgactcat ggctagttcc attttgagag actcgaagaa
    12601 cagtagctgt aagggcattc aatttcaagc ctttttcagt ccattcaagt cctttcgggt
    12661 tggcaattcc gactgatcct gcttggtttt catctgccaa aacttccgca tttcttagat
    12721 gtgcaaaggc agttcccaaa ttaaattcgc gagctttttc atccgcattg acaaagactg
    12781 cgtagatatc gccgtttgga gcagtgattt ggtagccaat cactacatcc tctttttcca
    12841 caccattttg gcctgggaca gtgatgaggt ggacacggtc tttgatatct tgaagactct
    12901 taagtcggaa ggcatctgta gattgacgaa gggcaatcaa acctttcata tagtcacggc
    12961 tcttgacatt ttcaggataa gctttaccat ctgtagcctt agtccagtca aacttgttga
    13021 ctgcatcgct agaatcgtaa gaatcatgga tgaagtaagg atagtcaaat gggttgccgt
    13081 ccttatcacg caacaagtga gatttgtttg gaaccttatc ctctgctact ggagtcttgt
    13141 aggctgggtc acggaattgt ttagtacgtc catattcctg accggagtgg ataaatggag
    13201 ttccttgagc tgtcaagacc attagatttc caagtcgtaa acgacggtgg atttcagcat
    13261 agttctcagc tttgcttggg tcttttttga tagactgggc aatgatgtca aagagggtca
    13321 agttatcatg ggctgcgatg tattggatga catctccagg gctgtcagct tcaaagttag
    13381 ttggctgagc aatgagattt ttaaagatgg tgttgacatc acgcttgcca cctgtgataa
    13441 aggcaggttg accttcgttt ggataaccag atttgaggtt gttacggatg tcatctgaaa
    13501 agacagcgac agtatcggta tgtttcatcc aatcttggtc agcagcttta gtaggcatgt
    13561 tttcatcacc ggcataggtt ctccaacctt cgcctaacat gattagattt ggattgaggg
    13621 cgcgtgcagc cttgtaagct tcttcgatag aagcggcgtc atggtctccc atcatatcga
    13681 aacggaatcc atccactttg taggtatcaa ctaggtattt gatagagtca actaggagcc
    13741 gtttggtcat atggtgggtt gtccccaagc gtccaccacc aaagctagtt cgaggtgtgc
    13801 catcggcatc cataaagtgg tagtagtttg gctctaggtc ttcaaagata tcgactttgg
    13861 ctgtgtggtt ataaacgaca tctaggatag ctcccatacc acgtttgtgg atttcgttga
    13921 tgaggttttt aaattctgcg attcgttttt ctggattctt aggatcgctt gagtacatac
    13981 cagtcaagga gaagtagttt tgagggtcat atccccagtt gtagttgctg ttgcttgaag
    14041 cgtagtcaga caagcgttca tggtttttca attcattgac aaagtagtaa gacaagactg
    14101 gaaggagctg gatatgggtt acacccaagt ctttgagata gtctagtttt tcaatgaagg
    14161 cttcaaaagt cccaaatggt ttggtcaagt cttttgcaat ggcaggatct gaagtgaagt
    14221 cacgcacatg agcttcgtag ataacggcgt cttcacgagt cttgaaattg tgaatcttac
    14281 cataagtcaa gtcttgaggt ccgagtttag ctggatctac aaaggcggct ttagccactt
    14341 tatgggcatc gtcaattttg gcatcgtcgc tattccaagc agcaagagat ttagcgtaag
    14401 gatcgagtgc aagaacagtt ttaccttgac gctcgatttg gtattgataa tagtagccag
    14461 tgaaatctgt gattccgagt ttgtttgtgc tgtctagagt ttgtttccaa gttcctcttt
    14521 cccctttttc gagagcgaca gttccaacta ctttgtcagg gtcattcttg tcgtagacaa
    14581 caacagaaac cttatcagca cttggtgacc aaagggtcaa atcaacttgt tttccttctt
    14641 cttttaggtc agctcccagt ttgccatcat agctgtatgt ctcatctttc aggcgccagc
    14701 ttgttttggt agtgaattgg tcggaattgt agctaacagt ataaggatgt tttgtgtcag
    14761 agaaatctcc gctgtaggtc actttcttac cagcttcatc gattgcaaca tcggtaatag
    14821 ttaccttgtt tcctaggtga ttagtgatgt tggagtgttt gaggatatct tcttttttag
    14881 caccgacaag tgttgaaaag ctactttcaa tgctagaagt gcctacgtgt tgggctcctg
    14941 tcatacggat atcatggaca tagtatggat ttgtgtaaat cgattcatca tcgtctttta
    15001 ggaaaatttg gctatgattt ttcaaatctg tgaacttata attttcttta cggattttca
    15061 cgtcgtctcc ttgtttgctc tcatctagta ataaaaatcc aaattctctt gcggcttcat
    15121 taagaggaat gtcgatatag cggccatatt tgcctgtagc cgtaaagtct gttccgtcag
    15181 gccattgagc gctacttgga ttttttacat ctccccagta ccagagagat ttcttgtcat
    15241 agttgccatc tgtgcggtag tagttgacgc gaacagttcc tgcaggctgt ggctcgtaag
    15301 agaaaacctt gtaatcttgg tctaaccaag cctcgttcat ttttggaact agtttttcta
    15361 cagatttatc gccggttaga ttttttccag ctgtattgtt gatgaggaag ctaattttct
    15421 tggcttgttc tccctttaat ttgacatcta ggtaatagcc gtagtcatct ttcttggcat
    15481 ccttgaagga caaagctccg tttggccagt tttcagatgg tttttcaaca tcgtcccaag
    15541 tccatagtcc ttgagcatcc ttgttttctt caggaagttt tttgacatgg atacggaagt
    15601 agttatcttc gatgggctct tctgccttag tttcagttgt tactggacta gtagaagtag
    15661 ttggctctgt tgcaggtttt tcttctgttt tctctaggga agcgtttgca tttctaggtg
    15721 aagaaatatc actttcgttc ttaccagagc cagctgctgt atcagtaaga ggttgggcaa
    15781 gggtggttgt atttctgttg ttgggttcgt tagtggttgg tgtactttcg tcggatgaga
    15841 tagttggtgt agccatggca agcaagacaa ggcttgcgcc gataaggaca gaaccagttc
    15901 catttttgag ggaacggatg ctgtagacca tttttttctc agtgtgagat ggtgtttttc
    15961 tcataatgat tctccaatca ataaatttct atatcagtat agcatgtagt aaaacaatat
    16021 gcaagcgttt tctttgattt gtgaaaaaaa ttcttacttt tgagaagttt tctgtactat
    16081 tttatatatc agtataaatg acggagataa aaatgtcaca taagccaagc cgtattatta
    16141 ggaagatgat gtgttcccag ctatttttat tcagaaattg atctctaaca ttaccaataa
    16201 aaaagaaaaa tatcttgaca agcaagggaa aattttgtta caataataga cggtactttt
    16261 tacttttggt ctctcaagag tgtacaggga cgtgctgaca aatgttgcaa aagtacacac
    16321 agatgatatc tgtcaccaag tgtatcatca ccaaaaataa aaaaacacag gagaatgtag
    16381 atgcctacaa ttaaccaatt ggttcgcaaa ccgcgtaaat caaaagtaga aaaatctaaa
    16441 tcaccagctt tgaacgttgg ttacaatagt cataaaaaag ttcaaacaaa cgtttcttca
    16501 ccgcaaaaac gtggtgttgc aactcgtgtt ggaacaatga cacctaaaaa acctaactca
    16561 gcccttcgta aattcgctcg tgtacgtttg agcaacctta tcgaagttac tgcctacatc
    16621 ccaggtatcg gacacaactt gcaagagcac agcgtggtgc ttcttcgcgg tggacgtgta
    16681 aaagaccttc caggggtacg ttaccatatc gtccgtggtg cacttgatac tgcaggtgtt
    16741 aacgatcgta aacaaggccg ttctaaatac ggtactaaac gtccaaaagc ataaggaaag
    16801 gggataaaga gaaatgagtc gtaaaaatag agctccaaaa cgtgacgtat tgccagatcc
    16861 gctatacaat tcacaactag ttactcgtct tatcaaccgc gttatgcttg atggtaaacg
    16921 tggtactgct gcttcaatcg tttacggtgc ttttgagcaa atcaaagaag ctactggcaa
    16981 cgatgcactt gaagtatttg aaacagctat ggaaaacatc atgcctgtac ttgaagtacg
    17041 tgcacgtcgt gttggtggtt ctaactacca agtcccagtt gaagttcgtc cagaacgtcg
    17101 tacaacactt ggacttcgtt ggttggtaac aatcgctcgt cttcgtggtg aacacacaat
    17161 gcaagaccgt cttgcaaaag aaatcttgga tgctgctaac aacactggtg cagcagttaa
    17221 gaaacgtgaa gatactcacc gtatggctga agctaaccgt gcattcgcac acttccgttg
    17281 gtaagatagg atgcgaaagc gttaagaaag tcccagagaa aatagggaat cgaagcaggt
    17341 tgcggttgca accaatgaga ttcatctttt tctccagact tttagcttga gctcaactaa
    17401 atcatgatgc taggaacggt aaggatgcaa ggtgaaaata ggaaactgac gcagtattcg
    17461 acgaatacaa ggagttttat ctttttcacg cagcatcccg ttccagctca catcggctaa
    17521 ctaactttag cccgggttca aattagctaa atcgattagt attagctata actcagctta
    17581 ccatctcgta agttgaaacc aacaatagca tgaaaacatt gagaacgggt aggtcctgcc
    17641 tatccgtttt tattaaaatc gtgttataat agaatagaaa tcaaaaataa ataggagaaa
    17701 caaacctcat ggcacgcgaa ttttcacttg aaaaaactcg taatatcggt atcatggctc
    17761 acgtcgatgc cggtaaaaca acaactactg agcgtattct ttactacact ggtaaaatcc
    17821 acaaaatcgg tgaaactcac gaaggtgcgt cacaaatgga ctggatggag caagagcaag
    17881 aacgtggtat cacgatcaca tctgctgcga caacagctca atggaacaac caccgcgtaa
    17941 acatcatcga cacaccagga cacgtggact tcacaatcga agtacaacgt tctcttcgtg
    18001 tattggatgg tgcggttacc gttcttgact cacaatcagg tgttgagcct caaactgaaa
    18061 cagtttggcg tcaagcaact gagtacggag ttccacgtat cgtatttgcc aacaaaatgg
    18121 acaaaatcgg tgctgacttc ctttactctg taagcacact tcacgatcgc cttcaagcaa
    18181 atgcacaccc aatccaattg ccaatcggtt ctgaagatga cttccgtggt atcattgact
    18241 tgatcaagat gaaagctgaa atctatacta acgaccttgg tacggatatc cttgaagaag
    18301 acatcccagc tgaatacctt gaccaagctc aagaataccg tgaaaaattg attgaagcag
    18361 ttgctgaaac tgacgaagaa ttgatgatga aatacctcga aggtgaagaa atcactaacg
    18421 aagaattgaa agctggtatc cgtaaagcga ctatcaacgt tgaattcttc ccagtattgt
    18481 gtggttcagc cttcaaaaac aaaggtgttc aattgatgct tgatgcggtt atcgactacc
    18541 ttccaagtcc acttgacatc ccagcaatca aaggtattaa cccagataca gacgctgaag
    18601 aaactcgtcc agcatctgac gaagagccat ttgcagctct tgccttcaag atcatgactg
    18661 acccattcgt aggtcgtttg acattcttcc gtgtttactc aggtgttctt caatcaggtt
    18721 catacgtatt gaatacttct aaaggtaaac gtgaacgtat cggacgtatc cttcaaatgc
    18781 acgctaacag ccgtcaagaa atcgacactg tttactcagg tgatatcgct gctgccgttg
    18841 gtttgaaaga tactacaact ggtgactcat tgacagatga aaaagctaaa atcatccttg
    18901 agtcaatcaa cgttccagaa ccagttatcc aattgatggt tgagccaaaa tctaaagctg
    18961 accaagacaa gatgggtatc gcccttcaaa aattggctga agaagatcca acattccgcg
    19021 ttgaaacaaa cgttgaaact ggtgaaacag ttatctcagg tatgggtgaa cttcaccttg
    19081 acgtccttgt tgatcgtatg cgtcgtgagt tcaaagttga agcgaacgta ggtgctcctc
    19141 aagtatctta ccgtgaaaca ttccgcgctt ctacccaagc acgtggattc ttcaaacgtc
    19201 agtctggtgg taaaggtcaa ttcggtgatg tgtggattga atttactcca aacgaagaag
    19261 gtaaaggatt cgaattcgaa aacgcaatcg tcggtggtgt ggttcctcgt gaatttatcc
    19321 cagcggttga aaaaggtttg gtagaatcta tggctaacgg tgttcttgca ggttacccaa
    19381 tggttgacgt taaagctaag ctttatgatg gttcatatca cgatgtcgac tcatctgaaa
    19441 ctgccttcaa gattgcggct tcactttccc ttaaagaagc tgctaaatca gcacaaccag
    19501 ctatccttga accaatgatg cttgtaacaa tcactgttcc agaagaaaac cttggtgatg
    19561 ttatgggtca cgtaactgct cgtcgtggac gtgtagatgg tatggaagca cacggtaaca
    19621 gccaaatcgt tcgtgcttac gttccacttg ctgaaatgtt cggttacgca acagttcttc
    19681 gttctgcatc tcaaggacgt ggtacattca tgatggtatt tgaccactac gaagatgtac
    19741 ctaagtcagt acaagaagaa attattaaga aaaataaagg tgaagactaa tccgtcctca
    19801 ctctagaagg aagtcactta gtggcttcct tttgtcttta gaaaatacct ctaaatatgg
    19861 taaaatagta gaagaataat gtgaggaaaa tgaatgtcaa atagttttga aattttgatg
    19921 aatcaattgg ggatgcctgc tgaaatgaga caggctcctg ctttagcaca ggccaatatt
    19981 gagcgagttg tggttcataa aattagtaag gtatgggagt ttcatttcgt attttctaat
    20041 attttaccga ttgaaatctt tttagaatta aagaaaggtt tgagcgaaga attttctaag
    20101 acaggcaata aagctgtttt tgaaattaag gctcggtctc aagaattttc aaatcagctc
    20161 ttgcagtcct actataggga ggctttctct gaaggtccat gtgctagtca aggttttaag
    20221 tccctttatc aaaatttgca agttcgtgct gagggcaatc agctatttat tgaaggatct
    20281 gaagcgattg ataaggaaca ttttaagaag aatcatcttc ctaatttagc caaacaactt
    20341 gaaaagtttg gttttccaac ttttaactgt caagtcgata agaatgatgt cctgacccaa
    20401 gagcaggaag aggcctttca tgctgaaaat gagcagattg ttcaagctgc caatgaggaa
    20461 gcgctccgtg ctatggaaca actggagcag atggcacctc ctccagcgga agagaaacca
    20521 gtctttgatt ttcaagcgaa aaaagctgca gctaaaccca agctggataa ggcggagatt
    20581 actcctatga tcgaagtgac gacagaggaa aatcgtctgg tatttgaagg ggttgttttt
    20641 gatgtggagc aaaaagtgac tagaacaggt cgtgttttaa tcaactttaa aatgacggac
    20701 tacacttcaa gtttttctat gcaaaagtgg gttaaaaacg aggaagaggc ccagaagttt
    20761 gatctcatca agaagaattc ttggctccga gttcgaggga atgtggagat gaataacttc
    20821 acacgcgatt tgactatgaa cgtacaggat ctgcaggaag ttgttcacta tgagcggaag
    20881 gatttgatgc cagaaggtga gcgtcgggtt gagtttcatg ctcatactaa catgtcgact
    20941 atggatgctt tgccagaggt cgaagagatt gttgcaacag ctgctaagtg gggacacaag
    21001 gcggttgcta tcacagacca tgggaatgtc cagtcctttc cacatggcta taaggcggct
    21061 aagaaagcgg gaatccagct gatctatggg atggaagcca atatcgtgga ggaccgtgtc
    21121 cctatcgtct ataacgaagt ggagatggac ttatcagaag caacctacgt ggtctttgac
    21181 gtggaaacga cgggactttc agctatctat aatgacttga ttcaggttgc ggcctctaag
    21241 atgtacaagg ggaatgttat tgctgaattt gatgaattta tcaatcctgg gcatcccttg
    21301 tcagctttta ctacagagtt aactggaatt acagatgatc atgtcaaaaa tgccaaacca
    21361 ctagaacaag ttttgcaaga attccaagaa ttttgcaagg atacggtcct agttgcccac
    21421 aatgctacct ttgacgttgg ctttatgaat gctaattatg agcgtcatga tcttccaaag
    21481 attagtcagc cagttattga tacgctggag tttgctagaa acctctatcc tgagtataaa
    21541 cgccatggtt tggggccttt gaccaagcgt tttggtgtgg ccttggaaca tcaccacatg
    21601 gccaactacg atgcggaagc gactggtcgt ctgcttttca tctttatcaa agaggtagca
    21661 gaaaaacatg gtgtgaccga tttagctaga ctcaacattg atctaatcag tccagattct
    21721 tacaaaaaag ctcggatcaa gcatgcgacc atctatgtca agaatcaggt aggtctaaaa
    21781 aatatcttta agctggtttc cttgtctaat accaagtatt ttgaaggagt gtcacggatt
    21841 ccgagaacgg ttctagatgc ccatcgagag ggcttgattt taggttcagc ctgttcagag
    21901 ggtgaagttt ttgacgtggt cgtttctcaa ggtgtggatg cggcggttga ggtggccaag
    21961 tattatgact ttatcgaggt catgccaccg gctatctatg cgcccttgat tgctaaagag
    22021 caggtcaagg atatggagga actccagacc attatcaaga gtttgataga ggttggagac
    22081 cgccttggca agcctgtttt ggttacggga aatgttcact atatcgaacc ggaagaagag
    22141 atttatcgtg aaattatcgt ccgtagtttg ggacagggtg cgatgattaa ccgaactatc
    22201 ggtcatggtg aacatgccca accagcacca cttccaaagg ctcattttcg aacgactaat
    22261 gagatgttgg atgaatttgc ctttttggga gaggaactgg ctcgtaaact ggttattgaa
    22321 aacaccaatg ccttggcaga aatatttgaa cccgttgaag tcgttaaggg tgacttgtat
    22381 acgcctttca tcgacaaggc tgaagaaaca gttgctgagt tgacctataa gaaagctttt
    22441 gagatttatg gaaatccgct gccagatatt gttgatttgc ggattgaaaa agaattaaca
    22501 tccatactgg ggaatggatt tgctgtgatt tatctggcat cgcagatgct ggtgcaacgt
    22561 tctaatgaac ggggttattt ggttggttct cgtgggtctg tcggatctag tttcgttgcg
    22621 accatgattg ggattacgga ggtcaatcct ctctctcctc actatgtctg tggtcagtgt
    22681 cagtacagtg agtttatcac agatggttcg tacggttcag gatttgatat gccccataag
    22741 gactgtccaa actgtggtca caaactcagt aaaaacggac aggatattcc gtttgagacc
    22801 ttccttggtt ttgatgggga taaggttcct gatattgact tgaacttctc gggagaagat
    22861 cagcctagcg cccacttgga tgtgcgtgat atctttggtg aagaatatgc cttccgtgcg
    22921 ggaacagttg gtacggtagc tgccaagact gcctatggat ttgtcaaggg ttacgagcga
    22981 gattatggca agttttatcg tgatgcagaa gtagaacgcc tcgctcaagg agcggcgggt
    23041 gtcaagcgga caacaggcca acacccgggg ggaatcgttg ttattccgaa ctacatggat
    23101 gtctacgatt ttacgcctgt ccagtatcca gcagatgatg tcacggctga atggcagacc
    23161 actcacttta acttccacga tatcgatgag aacgtcctca aactcgatgt actgggacat
    23221 gatgatccga ccatgattcg aaaacttcag gatttgtctg gtattgaccc taataaaatt
    23281 cctatggatg acgaaggcgt gatggcactc ttttctggga ctgatgtgct aggggtaaca
    23341 cctgaacaaa ttggaacgcc tacgggtatg ttggggattc cagagtttgg aacaaatttc
    23401 gtacgtggaa tggtagacga aacccatccg acaacctttg cggaattgct tcagctgtct
    23461 ggtctgtccc acggtactga tgtttggttg gggaatgctc aggatctgat taagcaagga
    23521 atagcggacc tatcgactgt tatcggttgt cgggacgaca tcatggttta cctcatgcat
    23581 gcgggtctgg aacctaagat ggcctttact attatggaac gggtacgtaa gggtttgtgg
    23641 ctaaagattt cagaagagga gagaaatggc tatatcgaag caatgaaggc taataaggtg
    23701 ccagagtggt atatcgaatc ctgtgggaaa attaagtaca tgttccctaa ggcccatgcg
    23761 gcagcctacg ttatgatggc cttgcgtgta gcttacttca aggttcacca tcctatttat
    23821 tactactgtg cttacttctc cattcgtgct aaggcttttg atatcaagac catgggtgcg
    23881 ggcttggagg tcatcaagcg cagaatggaa gaaatctctg aaaaacggaa gaacaatgaa
    23941 gcctctaatg tggaaatcga tctctataca actcttgaga ttgtcaatga gatgtgggaa
    24001 cgaggtttca agtttggtaa attagatctc tactgtagtc aggcgacaga gttcctcatc
    24061 gacggggata cccttatccc accatttgta gcaatggatg gtctgggaga gaacgttgcc
    24121 aagcaactgg tgcgggcgcg tgaagaggga gaattcctct ctaaaacaga actacgcaag
    24181 cgtggtggac tctcatcaac cttggttgaa aagatggatg agatgggtat tcttggaaat
    24241 atgccagagg ataaccagtt gagtttgttt gatgagttgt tttaaaaaat tgcttaataa
    24301 tctattaaaa gaggctaacg tatatccaat agatttacat tagctttctt ttttgttaaa
    24361 atagtctatg gaaagagggt gagagtatgt caaagatgag tataagcatc cgtctggata
    24421 gtgaggttaa ggagcaggcc caacaggtgt ttagtaatct gggaatggat atgacaacag
    24481 ctattaatat tttccttcgt caggcaattc aatatcaggg attacctttt gatgttagac
    24541 tagacgaaaa tcggaagttg ctccaagtgt taacggattt agaccaaaat cgtaatatga
    24601 gccagtcttt tgaatcagtc tcagatttga tggaggactt acgtgcttaa gattcgttat
    24661 cataaacagt ttaaaaaaga ttttaagttg gctatgaagc gtggtttgaa ggcagaatta
    24721 ttagaagaag ttttgaattt tctggttcaa gaaaaagaac atcctgccag atatcgtgat
    24781 cattcattga cggcatccaa gcattttcaa ggagttcgtg aatgccatac ccagccagat
    24841 tggcttttgg tttataaagt agacaagtcg gaattgattt taaatttgct gaggacaggc
    24901 agtcacagtg atttatttta atctatttta agggggttct catgaaacta agaatatttg
    24961 cggaagataa gccggctaag aaggtatttg aatatcaatt agaacttgct gatcgtacaa
    25021 ttcttctatc gacagcactc ttgtcaggtg ctattgcttt agcaggaatc ttttctgctt
    25081 tgaaagaaaa ataaaaatag aaaagagaaa acagaatggt tttaccaaat tttaaagaaa
    25141 atctagaaaa atatgcgaaa ttgttggttg cgaacggaat taacgtgcaa cctggtcaca
    25201 ctttggctct ctctattgat gtggagcaac gtgaattggc acatctaatc gtgaaagaag
    25261 cttatgcctt gggtgcgcat gaggtcatcg ttcagtggac agatgatgtg attaaccgtg
    25321 agaaattcct ccatgccccg atggagcgtt tggacaatgt gccagaatac aagattgctg
    25381 agatgaacta tctcttggag aacaaggcta gccgtcttgg agttcgttca tctgatccag
    25441 gtgccttgaa cggagtggat gctgacaagc tatcagcttc tgctaaagct atgggacttg
    25501 ccatgaaacc aatgcgaatc gcaactcaat ccaacaaggt tagctggacg gtagcagctg
    25561 ctgctggact tgagtgggct aagaaagtct ttccaaatgc tgcgagcgat gaagaagcag
    25621 tcgatctcct ttgggaccaa attttcaaaa cttgccgtgt ctatgaagaa gatcctgtta
    25681 aggcttggga aaatcatgca gctatcctta agagcaaggc tgatatgctt aataaagaac
    25741 aattttcagc ccttcactac acagcgccag ggactgattt gacccttggt ttgccgaaga
    25801 accacgtttg ggaatcagct ggtgctatca atgcacaggg cgaaggtttc ttgccaaata
    25861 tgccgacaga agaagtcttc acagctccag acttccgtcg tgcagatggt tatgtaacct
    25921 ctacaaaacc gcttagttat aacggaaaca ttattgaagg tattaaggtg acctttaagg
    25981 atggacaaat catagatatt actgctgaga agggtgatca ggttatgaag gatcttgtct
    26041 ttgaaaatgc gggtgcgcgt gccttgggtg aatgtgcctt ggtaccagat ccaagcccaa
    26101 tttctcagtc aggtattacc ttctttaaca ctcttttcga tgaaaatgcg tcaaatcact
    26161 tggctatcgg tgcagcctat gcgactagcg tcgttggtgg agcggagatg agcgaagagg
    26221 agcttgaagc tgcagggctt aaccgttcag atgttcacgt agactttatg attggttcta
    26281 accaaatgga tatcgatggt attcgtgagg atggaacgcg ggtacctctt ttccgtaatg
    26341 ggaattgggc aaattaagga gataatatgt taggaagtat gttcgttggt ctcctagtgg
    26401 gatttttagc aggtgctatg accaatcgtg gagagcgaat gggatgtttt ggaaaaatgt
    26461 ttctcggttg gatcggagcc tttctaggtc acttgctctt tggaacttgg gggccagttt
    26521 tatcaggaac agctattatc ccagcgattt taggagccat gattgtttta gctatttttt
    26581 ggagacgagg aagttaattt cctaaatctg atactcaatg aaaatcaaag agcaaactag
    26641 gaaactagcc gcaggttgct caaagcactg ctttgaggtt gcagatagaa ctgacgaagt
    26701 cagctaaaaa cactgttttg aggttgcaga tagaactgac gaagtcagta acatatatac
    26761 ggcaaggcga cgttgacgcg gtttgaagag atttttgaag agtatgaaac gaaaaggagg
    26821 ttggtcattg tactagcctc cttttgatat gatataatag ttctatgaga ttagataaat
    26881 ttttagttgc ctgtgcggtg ggaagtcgga ctgaggtcaa aaacttgctc aaggctgggc
    26941 gcgtgactgt aaatggtaaa aaagaaaaat cagctaaatt gcagattgat gaaaaaatag
    27001 atgagattcg ctttgatggg caagtgttgg agtatgaaga gtttgtctac tacatgatga
    27061 acaagcccaa aggagttatc tcagcgactg aggatcccaa gcacagaacc gttctggact
    27121 tgttggatga cttggcgcgg agtaaggaag tttttccagt aggacgcttg gatattgaca
    27181 cgcatggtct tttgctcttg accaataatg gtcagctggc tcatgttctt ctttcgccca
    27241 agcgtcatgt ggacaagact tatcaggctc aggtcaaggg aattatgacc caagaagatg
    27301 tggagacatt tgctgagggt attcctctca aagactttac ctgtcaaccc gctatactgg
    27361 agcttgtatc catagataca gaaaagaatc aaagccaaat ccgtgtgacc attgcagaag
    27421 ggaagtttca tcagatcaag cgtatggtgg gctactgtgg caaggaagtg gtggacttgc
    27481 aacgattgac tatgggaact ttagtattag atgagaacct ggaacgaggg gaatggcgtc
    27541 gcttgaccaa ggaagaatta gaaattctcc gcgctaatat tatttaattt agtttaatac
    27601 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gctgtacttg agtacggcaa
    27661 ggcgacgttg acgtggtttg aagagatttt cgaagagtat aaattcgaaa aaggtgaggg
    27721 acaatgtcct cgccttttat gttttttagt tgcttccttt gtgaaaagag ttataataga
    27781 ctgtagaata aaaggaggaa tctatgaacg cgattcaaga atcatttact gataaactat
    27841 ttgccaacta tgaagcaaat gtaaaatacc aagcgattga aaatgctgcc agccacaacg
    27901 gaatttttgc agctctggaa cgtcgccaaa gccatgtaga caacacacct gttttctcat
    27961 tggatttaac caaggacaag gtcactaacc agaaagcgtc tggtcgttgc tggatgtttg
    28021 cggctctcaa caccttccgc cacaaactca tctcgcaata caaattggag aactttgagt
    28081 tgtcacaagc ccacactttc ttctgggata agtatgagaa atcaaactgg ttcttggagc
    28141 aagtcattgc gacttcagac caagaattga ctagccgcaa ggttagcttc ttactccaaa
    28201 cacctcaaca agatggcggt caatgggata tggtcgtttc cctctttgaa aaatacggtg
    28261 tcgtgcctaa gtcagtttat cctgagtctg tttcatctag cagcagtcgt gagctaaatg
    28321 cgatccttaa taaattgctt cgtcaagatg ctcaaatctt gcgtgacttg cttgtttctg
    28381 gtgcagatca agcgactgtt caagctaaga aagaagacct cttgcaagaa atctttaact
    28441 ttcttgctat gtcattagga cttccaccac gcaagtttga ctttgcttat cgcgataaag
    28501 ataacaacta caaaagtgaa aaaggaatca caccacaaga gttttacaag aaatatgtca
    28561 atcttccttt agaagactac gtttctgtta tcaatgctcc aactgctgat aaaccttacg
    28621 gaaaatctta cacagttgag atgttgggga atgtggttgg tagccgtgca gttcgttaca
    28681 tcaacgttcc aatggagcgc ttgaaagaat tggcgattgc ccaaatgcaa gcaggtgaga
    28741 ctgtttggtt tggttctgat gtcggccagc tcagtaaccg taaggctggc atccttgcga
    28801 cagatgttta tgactttgaa tcaagcatgg acattaaact tactcaagac aaggctggac
    28861 gtttggacta tagcgaaagc ttgatgaccc acgccatggt cttgacaggt gttgacttgg
    28921 acgaaaatgg taaatcaacc aagtggaagg ttgaaaactc atggggagac aaggtcggta
    28981 cagatggtta ctttgttgcc tcagacgctt ggatggacga atacacatac caaatcgttg
    29041 ttcgtaagga attgctgaca gcagaagaac aagctgccta tggagcagaa ccaattgtac
    29101 ttgcaccatg ggatccaatg ggagccttgg ctgaataaaa gcatagaaaa aaggaatcag
    29161 attttagaac ctggttcctt tttagttgct tgattacatg atgtgaagaa catgtgccac
    29221 aatacccact gcgaagaggg caaggataat agtgattgga gatacttttt tcttaagtaa
    29281 gtacatgcaa agtaaagtaa ggagtagtcc tgataatcca ggaatcaaca tatccaagtt
    29341 ttgttggaaa gtagtaactt tttcaggagt ttgagacaat ccttgtccta cttgtgcgaa
    29401 tgcttcttgg atacctttag acccttctgg caatttatcc caatggatat aagccttttc
    29461 atctagttga actttagaaa catcgaaagc aaatttaata tttacccagc gttgaacaag
    29521 gacagcaaga atgaacatcc caaggataga agctccttta gtgatatctt gaaggatacc
    29581 accagacata tctttagtga tttctgatcc agccttgtat ccaatctctt gaacatacca
    29641 caagaatgac atacgaatca agttccatgc aacaaagaag aggagtggac ccaagatatt
    29701 gccagtaagg gcaagtgaag caccgagaga tccaaggatt gggcgtactg taaaccagaa
    29761 tactgggtca ccgataccag caagaggtcc catcataccg attttgaccc cttggatagc
    29821 agcgtcatcg atttccacac cgttagcacg ttcttcttca agcgcaagag taacccccat
    29881 gactggagca gctacgtatg gatgagtgtt gaagaactca aggtgacgct caagagcagc
    29941 gatttgatct tcttttttag tatagagttt tttgatagct ggaatgagtg tataagccca
    30001 gcccaagttt tgcatccgtt caaagttcca agacccttgt aagaaggttg aacgccacca
    30061 aactttttta cgatctgatt tagttaattg aagtttttca gtcatgatgt tttcagtcct
    30121 ttcttatctt agtagtcttc taggatatcg ccgattgggt cgttagaagt tgcggctcct
    30181 ccgccaccat ttccaccagt tttagaaagg tgaaggtaga taagagcgat agcaacaccg
    30241 atagcaccga atccgattag agtaatatct gacacagcag cgagaacgaa accaagagcg
    30301 aagaatggcc atacttcacg agttgccatc atgttgataa ccatggcgta accaacggca
    30361 acgaccatac caccaccgat agccatacca tctttgagcc agtctggcat ggcactaagg
    30421 atactttgta cagtttcagt tggtaccata aggagaagag ctgcaggaag cgcgatacga
    30481 agtccttgga agagtagcgc aatgaaatgc gcacgctcca cagcgccgaa gtcacctttt
    30541 ttagcggcag catctgcagt atgaaccaaa ccaactgaaa ttgtacgaac aatcattgtc
    30601 aagaaaagtc cagctacggc aagagggata gcaaccgctt gggcaacacc gataccagtc
    30661 ttggtaaagt caccaccaag aaccatgata atggcagcag cgacagaagc aagtgcagca
    30721 tcaggagcga tagcagcacc gatatttgac caaccaaggg caatcatttg aagcgatcca
    30781 ccgaggataa tccctgcttc caagtgacct gttacaagcc caataagggt acaggctaca
    30841 agtggttggt gaaattggaa ctggtcgagg atgccttcaa gacctgcaaa gaaggctaca
    30901 acgactacta aaaccataga aataatagac atgtttaaaa tcctttcata aataatggct
    30961 tatttgacat tggctttgtt aatcaagtca aacaaatctt ttttagaatc atttggtact
    31021 ttacggacat caaattcaac acccaagtca cgcatttttt caaatgtcgc aacatcttct
    31081 ttgtccatag acaaaacggt attgaccaat gttttacctg ttgagtgagc catagaacca
    31141 acgttaagag tcttgattgg cacgccgcct tcgatggcac gaagggcatc ttgaggtgtt
    31201 tcaaacaaga taagggcatg tgtttctcca aaacgtgggt cttttgaaat ctcaatcagt
    31261 ttttgaattg gaaccacgtt agccttgaca ttacctggag ctgcttgttt aatcaattct
    31321 ttacgaaggt cgtctttagc cacgttatct gaagcaacga tgatacgatt tgcttttgaa
    31381 tctggagtcc aagcagttgc aacctgaccg tgaagtagac gtgtgtcaag acgggcaaga
    31441 ttgattttca atttaccgtc tccgataaca gttccttctg ggatagcagc ttgggcaact
    31501 ggagcagctg cagcgcttgc aacttcttca actggattta gctcttctgg aagagctttg
    31561 atgccatcct tggcttcttt aatgatatta gcagcgactt tttctacacc tgcagcagcg
    31621 tccatgaggc gctctgtgta ggcttgaatc aacatcggta agttaagtcc tgtgatgatg
    31681 gcaaacttac gctcaggatt ttctcccatc acgcgactag cttggttaaa tggagaacca
    31741 ctccaaaggt cagccaaaac tagaacctca tcttctgcgt caaatgcagc aacagcgtta
    31801 ttaaacttag cgtatagatc atcaggacct tcatttggca taaaggtcac aacttgaacc
    31861 ttttcttgtt caccaaagat catagatcct gactgatgaa tacccgcagc aaattcgccg
    31921 tggctcgcaa taatgattcc gatactcatt attgtcattc ctcctttttt gttttagttt
    31981 tcatttaaga aaactttaag actgtttaag tataaaccgt tttcataaaa aaagcaacta
    32041 tttatcataa aaaattaaaa aatttttatt tttatatgtt gatat
//
LOCUS       DAVKHZ010000015        31842 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000015 DAVKHZ010000000
VERSION     DAVKHZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.015"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            186..620
                     /locus_tag="VWW99_000654"
     CDS             186..620
                     /locus_tag="VWW99_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9538533.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            632..1822
                     /locus_tag="VWW99_000655"
     CDS             632..1822
                     /locus_tag="VWW99_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9538534.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1833..2324
                     /locus_tag="VWW99_000656"
     CDS             1833..2324
                     /locus_tag="VWW99_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9538535.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELNQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2339..3118
                     /locus_tag="VWW99_000657"
     CDS             2339..3118
                     /locus_tag="VWW99_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9538536.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3105..3923
                     /locus_tag="VWW99_000658"
     CDS             3105..3923
                     /locus_tag="VWW99_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9538537.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3923..4216
                     /locus_tag="VWW99_000659"
     CDS             3923..4216
                     /locus_tag="VWW99_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9538538.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4238..6139
                     /locus_tag="VWW99_000660"
     CDS             4238..6139
                     /locus_tag="VWW99_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9538539.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNQEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEVLTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6199..7200
                     /locus_tag="VWW99_000661"
     CDS             6199..7200
                     /locus_tag="VWW99_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9538540.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7310..7645)
                     /locus_tag="VWW99_000662"
     CDS             complement(7310..7645)
                     /locus_tag="VWW99_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538541.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLTYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7656..7898)
                     /locus_tag="VWW99_000663"
     CDS             complement(7656..7898)
                     /locus_tag="VWW99_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538542.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7909..8451)
                     /locus_tag="VWW99_000664"
     CDS             complement(7909..8451)
                     /locus_tag="VWW99_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9538543.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIRNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(8467..8661)
                     /locus_tag="VWW99_000665"
     CDS             complement(8467..8661)
                     /locus_tag="VWW99_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9538544.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8827..9777
                     /gene="rsmH"
                     /locus_tag="VWW99_000666"
     CDS             8827..9777
                     /gene="rsmH"
                     /locus_tag="VWW99_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9538545.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9789..10106
                     /gene="ftsL"
                     /locus_tag="VWW99_000667"
     CDS             9789..10106
                     /gene="ftsL"
                     /locus_tag="VWW99_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9538546.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            10110..12362
                     /gene="pbpX"
                     /locus_tag="VWW99_000668"
     CDS             10110..12362
                     /gene="pbpX"
                     /locus_tag="VWW99_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9538547.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSIFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            12364..13344
                     /locus_tag="VWW99_000669"
     CDS             12364..13344
                     /locus_tag="VWW99_000669"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9538548.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13484..13672
                     /locus_tag="VWW99_000670"
     CDS             13484..13672
                     /locus_tag="VWW99_000670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538549.1"
                     /translation="MLEIDLTVLIDLSCSYFILLYSFEMVDRTDEVSSKHGFEVVDKE
                     KLMWFEEVFEECKKILVS"
     gene            13953..16058
                     /locus_tag="VWW99_000671"
     CDS             13953..16058
                     /locus_tag="VWW99_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9538550.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            16510..16845
                     /locus_tag="VWW99_000672"
                     /pseudo
     CDS             16510..16845
                     /locus_tag="VWW99_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(16912..17394)
                     /locus_tag="VWW99_000673"
     CDS             complement(16912..17394)
                     /locus_tag="VWW99_000673"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9538551.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(17489..18973)
                     /locus_tag="VWW99_000674"
     CDS             complement(17489..18973)
                     /locus_tag="VWW99_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9538552.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDNEAAVEASKQEIIRRYYQTILDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            19126..20733
                     /locus_tag="VWW99_000675"
     CDS             19126..20733
                     /locus_tag="VWW99_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9538553.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            20892..21065
                     /locus_tag="VWW99_000676"
     CDS             20892..21065
                     /locus_tag="VWW99_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9538554.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFGGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(21058..21904)
                     /locus_tag="VWW99_000677"
                     /pseudo
     CDS             complement(21058..21904)
                     /locus_tag="VWW99_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            22155..23600
                     /gene="cps4A"
                     /locus_tag="VWW99_000678"
     CDS             22155..23600
                     /gene="cps4A"
                     /locus_tag="VWW99_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9538555.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILASSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVLEIDDSSLAVVKAAIQDVMEGR"
     gene            23602..24333
                     /gene="cps4B"
                     /locus_tag="VWW99_000679"
     CDS             23602..24333
                     /gene="cps4B"
                     /locus_tag="VWW99_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9538556.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLIIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDKGCYT
                     QVNSSHILKSKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            24342..25034
                     /locus_tag="VWW99_000680"
     CDS             24342..25034
                     /locus_tag="VWW99_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEU9538557.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKRRLMILLVALVTGAGAFAYSTFVV
                     KPEYTSTTRIYVVNRNQEDKPGLTNQDLQAGTYLVKDYREIILSHDVLEKVATNLKLD
                     MPARALASKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFSFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLRIP
                     LLGLVPDLDKMK"
     gene            25044..25733
                     /locus_tag="VWW99_000681"
     CDS             25044..25733
                     /locus_tag="VWW99_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9538558.1"
                     /translation="MPTLEISQAKLDFVKKAEEYYNALCTNLQLSGDNLKVFSITSVK
                     LGEGKSTTSTNIAWAFARAGYKTLLIDGDIRNSVMSGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRKCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVDKYGSY
                     GNYGDYGKNKK"
     gene            25749..27116
                     /locus_tag="VWW99_000682"
     CDS             25749..27116
                     /locus_tag="VWW99_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9538559.1"
                     /translation="MNGKIVKSSLVIIQSFLVILLTYLLSAVREAEIVSTTAIALYIL
                     HYFVFYISDYGQAFFKRGYLIELVQTLKYILFFALAIGISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDATVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHVIAKRIIDIVGALVGLIL
                     CGLVSVVLVPLIRKDGGSAIFAQTRIGKNGRYFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            27148..28320
                     /locus_tag="VWW99_000683"
     CDS             27148..28320
                     /locus_tag="VWW99_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9538560.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTEYQKDGNIQYYVTCMR
                     ENSAKSGFTADTFEYNGAICYNIDVPNIGPARAIAYDIAAVNKAIELAKENKDEAPIF
                     YILACRIGPFISGLKKKIRSIGGRLLVNPDGHEWLRAKWSLPVRKYWKFSEQLMVKHA
                     DLLVCDSKNIEKYIREDYKQYQPKTTYIAYGTDTTPSSLKSEDAKVRNWYREKGVSEN
                     GYYLVVGRFVPENNYETMIREFIKSKSNKDFVLITNVEQNKFYDQLLKETGFDKDLRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     DGAIYWKKDELAHVIEEVERFDEGDISELDEKSSQRIADAFTWEKIVSDYEEVFKG"
     gene            28324..29481
                     /locus_tag="VWW99_000684"
     CDS             28324..29481
                     /locus_tag="VWW99_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9538561.1"
                     /translation="MKRILYLHAGAEMYGADKVLLELIKGLDSKEFETHVILPNDGVL
                     VEALRQVGAQVSVLDYPILRRKYFNPKGIFEYLKSYHRYSQKIAQYVRENGIVLVHNN
                     TTAVLEGIYLKRKVKLPLIWHVHEIIVKPKAISDFINFLMGRYANKIVTVSQAVASHV
                     NQSPFIKEGQVQVIYNGVDNAVYHPMPASTVREQFSIPEEALVIGMVGRVNAWKGQGD
                     FLEAVTPILEQNPNAIAFLAGSAFAGEEWRVEELESKIAKSSVVSQIKRIDYYEHTTE
                     LYNMFDIFVLPSTNPDPLPTVVLEAMACGKPVVGYRHGGVCEMVDEGTNGLLATPNQP
                     AELSKVIQELVENPEKRNQFGQASVERQRELFSLDSYIKNFSELYKTDRKD"
     gene            29484..30482
                     /locus_tag="VWW99_000685"
     CDS             29484..30482
                     /locus_tag="VWW99_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase WcwK"
                     /protein_id="HEU9538562.1"
                     /translation="MNKIDFVVTWVDGNDPVWKEKKSRYDGSVSTSKQSMNSVKAYRE
                     WGTFKYWFRGVERFAPWVNKVYLVTDQQRPSWLDINSEKLVLVDHTEIICNDCLPVFS
                     ANPIESNIHRIPGLSEHFVFFNDDMYLTAPVEPTDFFSEDGLPKYNTALSPIIPERYG
                     TGNFQINDMEIVTSYFSRNEILKNGQFFDPKQGLKSIVKSLLYRNSQFICGFWESHLP
                     YPLLKSTMDLIWEKEKAVLGRTSASRFRNPSDTNVWLFKYWQIASGQYAVGNPKLGRL
                     FSLDNAGPDFWNLLNSGKYKIMCINDVYDIKDEEKVMQEFIAAMKNLLPDKSTFEL"
     gene            30498..31475
                     /locus_tag="VWW99_000686"
     CDS             30498..31475
                     /locus_tag="VWW99_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9538563.1"
                     /translation="MPSVCALVVTYNRSKYLQKALEGILNQQQEISGILIYNNNATDN
                     TEEILMKFGYVDSKGDKIKENYLYSTEKDGKHFYYYHNDENLGGAGGFANGIRLISEL
                     DYDYVWIMDDDVYPEPNCLSEIMKQMSVQNVQVGIPNRTDENFDDRAIIGFDFDDYHK
                     FWTEMRKTVTYGPFDEEAIKVVDMPFEGPVVEMALLRKVGIPDSGFFIEYDDSDFAQR
                     LQQYSDIIFATKAQLHRQLAVKVDPSEVKKVEPYNWRNYYKIRNNIIFDKRYGKNWKV
                     RQLSPLILIAHHIVIAIRHQHLKHNLPIIWKGFWDGVFQRMGKRVDPNY"
     gene            31488..>31842
                     /locus_tag="VWW99_000687"
     CDS             31488..>31842
                     /locus_tag="VWW99_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9538564.1"
                     /translation="MSDLLLSFIMPTYNSAEYLADTMDSLVASIGDYNHLVEIICVDD
                     GSWDNTVTILEGYKSIYDNLIIIRNEHGGVSQARNTALDIVSGKYISFVDSDDLYEKD
                     FLNTFISLDKNFDFLF"
BASE COUNT         9739 a         5547 c         7152 g         9404 t
ORIGIN      
        1 ttatttataa aaatatttat tttccttaaa aatggaatat tttgaaattt ctgtctatta
       61 aaatgattaa aaagataatg aaagcgctaa tattttctta aaaaaactat tgacaagata
      121 aaaataaagg attacaatgg ggaatataaa gtaaaccggt aaacctaaac aagaaagggg
      181 aaaagatgaa aattgtactt gtagggcatg gacattttgc tacagggatt tatagttctt
      241 tacaattgat tgcaggcaat caagaaaatg tggaggcgat tgactttgtg gaaggaatgt
      301 cagcagatga actcaagcaa aaaatcttac ttgcaatttc aaatgaagaa gaagttttaa
      361 tcctaagtga tctcttggga ggatcgccat tcaaggtttc ttctaccata atgggagaaa
      421 atccagccaa aacaatgaat gttctctcgg gtttgaactt agccatgtta atggaagcag
      481 tctttgctag aatggctcat agctttgatg aagttgttaa taaatcagta gtggcggccc
      541 agggcggagt cgtaaatggt aaagaattgt tttcaacgga tgcagaggaa gaggaagaag
      601 atttcgaatc gggtatttaa agggtaaaag aatgataaaa aaggttacga ttgaaaaaat
      661 aaaatcgcct gagcgcttct tagaagtacc acttctgacg aaagaagaag tcggccaggc
      721 aatcgataag gttattcggc agttagaact caaccttgac tatttcaagg aagatttccc
      781 gacaccagct acctttgata atgtctatcc aatcatggat aacacggaat ggaccaatgg
      841 tttctggaca ggagaactgt ggttggctta tgaatacagt caacaggatg catttaaaaa
      901 catcgctcat aaaaatgttc tttctttcct ggatcgtgtc aataagagag tagaattgga
      961 tcaccatgat ctcggcttct tgtacacacc gtcttgtatg gctgaatata agataaatgg
     1021 agatggagag gctagagaag cagccttgaa agctgcagat aagttgattg aacgctatca
     1081 agaaaaaggt ggttttattc aagcttgggg agacttgggc aagaaagagc attaccgttt
     1141 gattatcgat tgcttgctca atatccaact cttattcttt gcttatcaag aaacaggcga
     1201 tcaaaaatac tacgatattg cagaaagcca tttctatgct tcagctaata atgtaatccg
     1261 tgatgacgct tcgtccttcc acaccttcta ttttgatcct gagacaggtc aaccctttaa
     1321 aggtgtaacg agacaagggt atagtgatga ttcatgctgg gcacgtggtc agtcatgggg
     1381 agtttatggt attcctttga cttatcgtca cttaaaagac gagtcctgct ttgacttgtt
     1441 taagggtgtg accaattatt tcttgaatcg tctaccaaaa gatcatgtgt cctattggga
     1501 tttgattttt aatgatggta gtgatcaatc acgagattct tcagcaacag ctatcgccgt
     1561 ctgtgggatt catgaaatgc taaaacatct cccagaggtg gatgctgaca aagatattta
     1621 taaacatgct atgcatgcca tgcttcgttc cttgatcgaa cattatgcaa atgatcaatt
     1681 tacccctggt gggacaagtc tcctccacgg tgtgtactca tggcattcag gtaaaggagt
     1741 ggatgaaggc aatatctggg gtgactacta ttacctagaa gcccttatcc gtttctacaa
     1801 agactggaac ctatattggt aggaggagaa atatgacaat gccaaatatt attatgaccc
     1861 gtatcgatga acggttgatt catggacaag gacaactttg ggtaaaatac ctaggttgta
     1921 atacggtcat tgttgccaat gacgaagtaa gcacggacaa gatgcaacaa actctgatga
     1981 aaacagttgt gccagactca gttgccatgc gtttcttccc tttgcaaaag gtgattgata
     2041 tcattcacaa ggctaatcct gctcaaacga tctttatcgt tgtaaaggat gtgaaggacg
     2101 ctttaacctt ggtagaaggt ggtgtcccta tcaaagaaat caatattggg aacattcaca
     2161 atgctactgg taaagagcaa gtgacacgct ccatcttcct gggtgaagag gacaaggcgg
     2221 ccctcaagga attgaaccaa actcatcaag taacatttaa tacgaaaaca actccaacag
     2281 gaaatgatgg agctgttcaa gtcaacatta tggactatat ttaaaagagg agatcgttat
     2341 gtcgattaat gtatttcaag cgattttaat tggattatgg acagctttct gttttagtgg
     2401 aatgctgtta ggaatttaca ccaatagatg tattgttctg tcatttggtg tcggaattat
     2461 tctaggtgat ctgcctactg ctcttgcaat gggagctatt ggtgaattgg cttatatggg
     2521 attcggtgtt ggtgctggag gtactgttcc accaaaccca atcggacctg gtatctttgg
     2581 taccttgatg gctatcacta gtgctggtaa agtcagtcca gaagcggctc ttgccctctc
     2641 tactccgatt gctgtggcga ttcaattctt acaaactttc gcctacactg tacgtgctgg
     2701 tgcgcctgaa acagcgatga agcacttgaa aaaccataat ttgaagaaat ttaagttcac
     2761 tctaaatgca acgatttggt tgtttgcctt tattggattt accttgggtt gcttgggtgc
     2821 cctttcaatg gataccttgt tgaaactcgt agactacatt ccaccggtat tgcttacagg
     2881 tttgacagtt gctggtaaaa tgctcccagc tatcgggttt gcgatgatat tgtcagtgat
     2941 ggctaagaaa gagttgattc cctttgtctt gttgggatat gtttgtgcag cttatctaaa
     3001 catcccaaca attggtattg caattgtagg tactatcttt gctttgattg aattttataa
     3061 caagccaaaa acagcggatc atgtggtaga ggaggaagca cacgatgact ggatctaaca
     3121 aattaacaaa acgtgattat cttaaaacgt ctttgcgggc attcttttgt caaaatggat
     3181 ttaactatag taactatcaa gggttgggat atgccaatgt gatgtatcct gctttgaaaa
     3241 aacactatgg agaggatcag gaaggtttct accaagcctt ggaagaaaac tgtgaattct
     3301 ataataccaa cccacacttc ctgcctttta ttaccagctt gcatcttgta atgttggaaa
     3361 atggccgtcc ggcaaaagaa acacgtagca tcaagatggc cttgatggga ccattggcag
     3421 gtattgggga ttctctttct caattctgtt tagctccatt gttctcaacc atcgcagctt
     3481 cgtttgctca agaaggcttg gttgtcggtc caatcttgtt cttccttgcg atgaatacga
     3541 ttttaacagc gattaaattg tcaactggtc tgtatggata caaactagga acaactgtga
     3601 ttgataaact aagcgagcag atggcaacga tttctcgtat tgccaatatt atcggtgtaa
     3661 ccgtaattgc tggtttggca gcgacatctg ttaagattat ggtgccgatt acctttgctg
     3721 caggggaagt taaagcagac gctaaacaaa gtatcgtaag tattcaggga atgcttgata
     3781 aggttgctcc agctcttcta ccagccctat ttacactttt agtttactac ttgatcaaag
     3841 aaaagaaatg gacaacatat aaactcgtta ttttaacagt tatcatcgga attatcggaa
     3901 gctggcttaa gattatagct taatggatac aacattgttt tatggaatag tgattgtctt
     3961 ggcagtgagt ccccttttac tgtcaagctt tcattctatt cgtcagcaaa agttgcttcg
     4021 caaacagatg gagcaacgac aagagtattt agcttcttta acatctggtg atgaagtgtt
     4081 gttattgtct ggaattcatg gaaaaatcat ttctatcaaa gatgacttgg tctccttgca
     4141 gattgcaaaa ggtgtggtca tctatgtaga aaaggaaagt gtaatgggaa agacaaaaga
     4201 actgcttttt aagtagttct ttttctggta atagataatg aaagaagaaa gaagacaatt
     4261 ttttgaaaga gtcgatggaa accaatgtcg tgattatatc ttgtcccact gttcaaaaga
     4321 ctatgagaaa gtcaagagtt cccttgaacg cttgatggac aatcgtttta tgtttgatag
     4381 tccttgggat atggagcctt gttcaaaaat ccatcaaatc cagccgatgg tatgggatca
     4441 agtatttgaa gatgatccag aatggtctta tatgctcaat cgacaagaat atctcttgca
     4501 gtttatgata gggtatctgg tagaaggaga taaggactat attcaaaagt gcaagttctt
     4561 tctatttgat tggattgagc aggtgagaga attttctcct caatccttga tgactagaac
     4621 cttggatacg ggtattcgtt cctttacttg gttgaaacta ctcttgctcc tcttgaaatt
     4681 tgacttgcta gaggagaaag aactagagaa aattttggtc agtctagaaa agcagattga
     4741 ctttatgaaa agccactatc gcgccaagta cacccttagt aactggggga ttttacaaac
     4801 aattccgatg cttgctatct attatttctt ttcagataag atggacctag aagaagctta
     4861 ccattttgct tcagaggagt tgaaacagca aattgagaca cagattttag gagatggaag
     4921 ccagtttgaa cagtcgattc tctatcatgt agaggtttat aaagccttgc tggatttgtg
     4981 tctcttgctt ccagacttgc aagatagtta ccaagagttg ctggaaaaga tggcgaccta
     5041 tattcaaatg atgacaggct tagatggacg gactttggct tttggtgata gcgattctac
     5101 agaaacgaca gaaatgttga gcctgtctgc tgtggttttg aaccaggaag accttcttaa
     5161 cggtctggat gttaaagttg atttgcttag cctcttgttc ctggggcgag aaaaggtcaa
     5221 gcgactgcag gaatttgaaa agagagcttg gcagcctaag tccatgatct ttgaagactc
     5281 tggacatgtc tgcattaagg atgaacatcg ttatctattt ttcaaaaatg gtccgctagg
     5341 aagtgcccat agtcatagtg acgagaatag tttttgctta cagtatcaag gccaacctat
     5401 tttcatagat gctgggcgtt attcttatcg ggagatatat gaacgttatc tcttaaagag
     5461 tgcttggagt cattcgacct gcattgtaga tgggaaagct ccggaaagaa tcacgggatc
     5521 ctgggaatat gaatactatc ctcactccct gttttgtcac cataaagaaa gggagggagt
     5581 gcattatatt gagggggctt attggtcagc agaacctgat ttgccttatc ttcacaggag
     5641 aaaaatcctc atgttggtag aggatgtctg gctcttggta gatgacatca ggtgtcaagg
     5701 tcaacatgag gtgttgactc agtttatcct tgacaaggat gtgacctatc aagatgggaa
     5761 aatcaatcag ttgagactat ggagtgaagt tgattttgat ttggaagata ccatcatttc
     5821 tcctaaatac aatgagcttg aaagaagtag caaactcacc aagcgccaat tctttgagaa
     5881 tcagatgctg gattatacca tcattgcgca tgagagtttt gaaatcatcc gtcattctgt
     5941 ctaccagaca gatgatcgtg aagtggaaaa tgctctggct tttgaagtga aaaatgatga
     6001 aacagacaag ctgattctgt tattaagcga ggatattggt gtaggtgaaa aattgtgcct
     6061 cgttgacgga acaaaaatgc gtggaaaatg tttagtatat gataaaataa atgagagaat
     6121 gattcgcttg cagtgctaga aataggcatt ttgaatagtg aatatgttat aataagtatt
     6181 agtaggaggt gttttagatt ggagaagaaa ctgaccataa aagacattgc ggaaatggct
     6241 cagacctcga aaacaaccgt gtcattttac ctaaacggga aatatgaaaa aatgtcccaa
     6301 gagacacgtg aaaagattga aaaagttatt catgaaacaa attacaaacc gagcattgtt
     6361 gcgcgtagct taaactccaa acgaacaaaa ttaatcggtg ttttgattgg tgatattacc
     6421 aacagtttct caaaccaaat tgttaaggga attgaggata tcgccagcca gaatggctac
     6481 caggtaatga taggaaatag taattacagc caagagagtg aggaccggta tattgaaagc
     6541 atgcttctct tgggagtaga cggctttatt attcagccga cctctaattt ccgaaaatat
     6601 tctcgtatca tcgatgagaa aaagaagaaa atggtctttt ttgatagtca gctctatgaa
     6661 caccggacta gctgggttaa aaccaataac tatgatgccg tttatgacat gacccagtcc
     6721 tgtatcgaaa aaggttatga acattttctc ttgattacag cggatacgag tcgtttgagt
     6781 actcggattg agcgggcaag tggttttgtg gatgctttaa cagatgctaa tatgcgtcac
     6841 gccagtctaa ccattgaaga taagcatacg aatttggaac aaattaagga atttttacaa
     6901 aaagaaatcg atcccgatga aaaaactctg gtatttatcc ctaactgttg ggccctacct
     6961 ctagtcttta ccgttatcaa agagttgaat tataacttgc cacaagttgg gttgattggt
     7021 tttgacaata cggagtggac ttgcttttct tctccaagtg tttcgacgct ggttcagccc
     7081 tcctttgagg aaggacaaca ggctacaaag attttgattg atcagattga aggccgcaat
     7141 caagaagaaa ggcaacaagt cttggattgt agtgtgaatt ggaaagagtc gactttctaa
     7201 aatgaaggaa aatgacttgc aatttctgtt aagaaataaa ataatcccac ctagaacaag
     7261 ctaggtggga ttatttgcct atgaaatgag aaattatggg agcaagctcc taaatcaact
     7321 gtttttgatc tacttcttta actacttgat aaaagttata gaagtaggtc aaacttgaaa
     7381 tgatggttac gactaggaat attgaaaatt tccattggac agggttggtt aaaagttgtg
     7441 gaaaggatat gaggagaaag aagagggctg cgttgaggac aggtatccgt tttgattgta
     7501 ttttctcaag tcctttattg agcgcaggaa gaaagaggag taggagtagt aaaactgtat
     7561 gagaaacagc tcctgaaata agggcgaaga aaaggaaaat actgataaaa acatgaatga
     7621 tcagtagtct agctagtgat ttcataaggc acctcctaat cctggtcttt tttagctctt
     7681 gcaatacgaa gtgagtcgac aatatgtatc atcactccga aaaagaaagc tcccagtata
     7741 gttttaaaaa tatgttttgt atttagaaga gaactgataa aatttggatt ttcacttgtt
     7801 agggtatcaa tgagtggaat tataaaaaat atcactgttc cataaatcga acctgctttc
     7861 agacctggat aacgtaactg tctcttttct tttttcatag ggcacctcct aatcctcatc
     7921 ttgatttttc ttagtttttg caatgcgacg ggagatgagg aactgtatgc tcgctccgaa
     7981 gaaaatagaa ccgagaatgc ttgatacacc atttcttata gtgagaagag aatgaaaata
     8041 gtcctgacct tcacctatga gtatcctgag aagaggagtt ataaaaaaca tccatagacc
     8101 aaagaacaaa cctgctttca gacctgggta gtgtagttgc ttgctttctt tctcattcag
     8161 catatctggt tcaatgactg tgatgcctgt ttttttcatt tggtaggtga catagccagc
     8221 agcgatgagg gcaatcacta aaatcagagg aggatagatt agagccactt cttgagggta
     8281 tttataggcc agaaggagtg gaataagatt tccgaaaatc atcagataaa agaggacgat
     8341 aaaaacttgg ttccgaatac tatcggcctc acgccgtttg tattcgtcaa ggggaccaga
     8401 aataccgtat gtgcgtttga tcagtttttc agtgaaggtt tcttttttca taagtttgct
     8461 ccttttttaa aaatcttcct cccaaaagag actgttgagg tcagtttgga ggctgcgggc
     8521 gagattgaga cagagttcca aggttggatt gtacttgtcg ttttcaatca tattgatagt
     8581 ctgtctcgag acaccgatat ccttggcgag ttcgagctgg gaaataccca attccttgcg
     8641 aaattctttc acacgattca tctgttctcc tttctgattt atgtcgtata tatttgacta
     8701 tattatagtc ttttaaacat aaagtgtcaa gtatttttga catatttttt gaagaaatag
     8761 tagtctcctt gtcctatttg tctgacaagt gcaagctggt cggatttgtg gtaaaataga
     8821 taagatatga caaaagaatt tcatcatgta acggtcttac tccacgaaac gattgatatg
     8881 cttgacgtaa agcctgacgg tatctacgtt gatgcgactt tgggcggagc aggccatagc
     8941 gagtatttat taagtaaatt aagtgaaaaa ggccatctct atgcctttga ccaggatcag
     9001 aatgccattg acaatgcgca aaaacgcttg gcaccttaca ttgagaaggg aatggtgacc
     9061 tttatcaagg ataacttccg tcatttacag gcacgtttgc gcgaagctgg tgttcaggaa
     9121 attgatggaa tttgttatga cttgggagtg tctagtcctc aattggacca gcgtgagcgt
     9181 ggtttttctt ataaaaagga tgcgccactg gacatgcgga tgaatcagga tgctagtctg
     9241 acagcctatg aagtggttaa tcattatgac tatcatgact tggttcgtat tttcttcaaa
     9301 tacggtgagg ataaattctc taaacagatt gcgcgtaaga ttgagcaagc gcgtgaagtg
     9361 aagccgattg agacaacgac tgagttagca gagattatca agttggtcaa acctgccaag
     9421 gaactcaaga agaagggtca tcctgctaag cagattttcc aggctattcg aattgaagtc
     9481 aatgatgaac tgggggcggc agatgagtcc atccagcagg ctatggatat gttggctctg
     9541 gatggtagaa tttcagtgat tacctttcat tccttagaag accgcttgac caagcaattg
     9601 ttcaaggaag cttcaacagt tgaagttcca aaaggcttgc ctttcatccc agatgatctc
     9661 aagcccaaga tggaattggt gtcccgtaag ccaatcttgc caagtgcgga agagttagaa
     9721 gccaataacc gctcgcactc agccaagttg cgcgtggcca gaaaaattca caagtaagag
     9781 ggaaaaagat ggcagaaaaa atggaaaaaa caggtcaaat actacagatg caacttaaac
     9841 ggttttcgcg tgtggaaaaa gctttttact tttccattgc tgtaaccact cttattgtag
     9901 ccattagtat tatttttatg cagaccaagc tcttgcaagt gcagaatgat ttgacaaaaa
     9961 tcaatgcgca gatagaggaa aagaagaccg aattggacga tgccaagcaa gaggtcaatg
    10021 aactattacg tgcagaacgt ttgaaagaaa ttgccaattc acacgatttg caattaaaca
    10081 atgaaaatat tagaatagcg gagtaagata tgaagtggac aaaaagagta atccgttatg
    10141 cgaccaaaaa tcggaaatcg ccggctgaaa acagacgcag agttggaaaa agtctgagtt
    10201 tattatctat ctttgttttt gccatttttt tagtcaattt tgcggtcatt attgggacag
    10261 gcactcgctt tggaacagat ttagcgaagg aagctaagaa ggttcatcaa accacccgta
    10321 cagttcctgc caaacgtggg actatttatg accgaaatgg agtcccgatt gctgaggatg
    10381 caacctccta taatgtctat gcggtcattg atgagaacta taagtcagca acgggtaaga
    10441 ttctttacgt agaaaaaaca caatttaaca aggttgcaga ggtctttcat aagtatctgg
    10501 acatggaaga atcctatgta agagagcaac tctcgcaacc taatctcaag caagtttcct
    10561 ttggagcaaa gggaaatggg attacctatg ccaatatgat gtctatcaaa aaagaattgg
    10621 aagctgcaga ggtcaagggg attgatttta caaccagtcc caatcgtagt tacccaaacg
    10681 gacaatttgc ttctagtttt atcggtctag ctcagctcca tgaaaatgaa gatggaagca
    10741 agagcttgct gggaacctct ggaatggaga gttccttgaa cagtattctt gcagggacag
    10801 acggcattat tacctatgaa aaggatcgtc tgggtaatat tgtacccgga acagaacaag
    10861 tttcccaacg aacgatggac ggtaaggatg tttatacaac catttccagc cccctccagt
    10921 cctttatgga aacccagatg gatgcttttc aagagaaggt aaaaggaaag tacatgacag
    10981 cgactttggt cagtgctaaa acaggggaaa ttctggcaac aacgcaacga ccgacctttg
    11041 atgcagatac aaaagaaggc attacagagg actttgtttg gcgtgatatc ctttaccaaa
    11101 gtaactatga gccaggttcc actatgaaag tgatgatgtt ggctgctgct attgataata
    11161 atacctttcc aggaggagaa gtctttaata gtagtgagtt aaaaattgca gatgccacga
    11221 ttcgagattg ggacgttaat gaaggattga ctggtggcag aatgatgact ttttctcaag
    11281 gttttgcaca ctcaagtaac gttgggatga ctctccttga gcaaaagatg ggagatgcta
    11341 cctggcttga ttatcttaat cgttttaaat ttggagttcc gacccgtttc ggtttgacgg
    11401 atgagtatgc tggtcagctt cctgcggata atattgtcaa cattgcgcaa agctcatttg
    11461 gacaagggat ttcagtgacc cagacgcaaa tgattcgtgc ctttacagct attgctaatg
    11521 acggtgtcat gctggagcct aaatttatta gtgccattta tgatccaaat gatcaaactg
    11581 ctcggaaatc tcaaaaagaa attgtgggaa atcctgtttc taaagatgca gctagtctaa
    11641 ctcggactaa catggttttg gtagggacgg atccggttta tggaaccatg tataaccaca
    11701 gcacaggcaa gccaactgta actgttcctg ggcaaaatgt agccctcaag tctggtacgg
    11761 ctcagattgc tgacgagaaa aatggtggtt atctagtcgg gttaaccgac tatattttct
    11821 cggctgtatc gatgagtccg gctgaaaatc ctgattttat cttgtatgtg acggtccaac
    11881 aacctgaaca ttattcaggt attcagttgg gagaatttgc caatcctatc ttggagcggg
    11941 cttcagctat gaaagactct ctcaatcttc aaacaacagc taaggcttta gagcaagtaa
    12001 gtcaacaaag tccttatcct atgcctagtg tcaaggatat ttcacctggt gatttagcag
    12061 aagaattgcg tcgcaatctt gtacaaccca tcgttgtggg aacaggaacg aagattaaaa
    12121 acagttctgc tgaagaaggg aagaatcttg ccccgaacca gcaagtcctt atcttatctg
    12181 ataaagcaga ggaggttcca gatatgtatg gttggacaaa ggagactgct gagacccttg
    12241 ctaagtggct caatatagaa cttgaatttc aaggttcggg ctctactgtg cagaagcaag
    12301 atgttcgtgc taacacagct atcaaggaca ttaaaaaaat tacattaact ttaggagact
    12361 aatatgttta tttccatcag tgctggaatt gtgacatttt tactaacttt agtagaaatt
    12421 ccggccttta tccaatttta tagaaaggcg caaattacag gccagcagat gcatgaggat
    12481 gtcaaacagc atcaggcaaa agctgggact cctacaatgg gaggtttggt tttcttgatt
    12541 acttctgttt tggttgcttt ctttttcgcc ctatttagta gccaattcag caataatgtg
    12601 ggaatgattt tgttcatctt ggtcttgtat ggcttggtcg gatttttaga tgactttctc
    12661 aaggtctttc gtaaaatcaa tgaggggctt aatcctaagc aaaaattagc tcttcagctt
    12721 ctaggtggag ttatcttcta tcttttctat gagcgcggtg gcgatatcct gtctgtcttt
    12781 ggttatccag ttcatttggg atttttctat attttcttcg ctcttttctg gctagtcggt
    12841 ttttcaaacg cagtaaactt gacagacggt gttgacggtt tagctagtat ttccgttgtg
    12901 attagtttgt ctgcctatgg agttattgcc tatgtgcaag gtcagatgga tattcttcta
    12961 gtgattcttg ccatgattgg tggtttgctc ggtttcttca tctttaacca taagcctgcc
    13021 aaggtcttta tgggtgatgt gggaagtttg gccctaggtg ggatgctggc agctatctct
    13081 atggctctcc accaagaatg gactctcttg attatcggaa ttgtgtatgt ttttgaaaca
    13141 acttctgtta tgatgcaagt cagttatttc aaactgacag gtggtaaacg tattttccgt
    13201 atgacgcctg tacatcacca ttttgagctt gggggattgt ctggtaaagg aaatccttgg
    13261 agcgagtgga aggttgactt cttcttttgg ggagtgggac ttctagcaag tctcctgacc
    13321 ctcgcaattt tatatttgat gtaagaatgg caccctgatg tttcagggtg tttttgtgtt
    13381 taaatacaca atgaaaatca aagaacaaac tagaaagcta actttaggct gctcaaaata
    13441 tagtagattg aaactaaaat agtacacatc tacttctaaa atattgttag aaatcgattt
    13501 gactgtcctg attgatttgt cctgttctta tttcatttta ctatacagtt ttgagatggt
    13561 tgatagaact gacgaagtca gctcaaaaca cggttttgag gttgtagata aggagaagct
    13621 gatgtggttt gaagaggttt tcgaagagtg taaaaaaata ttggtcagtt aaagtcaaag
    13681 ctcttgacct tttctgacca atgtagtata ttatgaacgt aaggtaaatg aaagccttac
    13741 tagaacactt atttaaagta aaatagaaag aggtgggtct atgtttcatc tagaaatctt
    13801 cagaagtaaa gatagtctac tcctgcttga aaaagaaaaa ccagaaatag tacgtagggt
    13861 agcgatctag ctagtctaaa ttttttaaaa caatggtcaa agatagtcaa tatcagtaat
    13921 cataactaag taaacaaaaa gaggtaaaga atatgaataa caactttaat aattttaata
    13981 acatggatga tttatttaac caattgatgg gtggtatgcg aggatacagt tctgaaaatc
    14041 gccgttactt aattaatgga cgcgaagtca cacctgagga atttgctcac tatcgtgcga
    14101 ctggtcaatt accaggaaat gcagaaactg atgtgcaaat gccacaacag gcatcaggta
    14161 tgaaacaaga cggtgtcctt gcaaaactag gtcgaaactt aacagcagaa gcgcgtgagg
    14221 gcaagttgga tcctgttatc ggacgaaaca aggaaattca agaaacatct gaaatcctct
    14281 cacgccgcac caagaacaat cctgttttgg tcggagatgc aggtgttggt aagacagcag
    14341 ttgtcgaagg tctagcgcaa gccattgtga acggagatgt tcctgctgct atcaagaaca
    14401 aggaaattat ttctattgat atctcaggtc ttgaggctgg tactcaatac cgtggtagct
    14461 ttgaagaaaa tgtccaaaac ttagtcaatg aagtgaaaga agcagggaat attatcctct
    14521 tctttgatga aattcaccaa attcttggtg ctggtagcac tggtggagac agtggttcta
    14581 aaggacttgc ggatattctc aagccagctc tctctcgtgg agaattgaca gtgattgggg
    14641 caacaactca agacgaatac cgtaacacca tcttgaagaa tgctgctctt gctcgtcgtt
    14701 tcaacgaagt gaaggtcaat gctccttcgg cagagaatac ttttaaaatt cttcaaggaa
    14761 ttcgtgacct ctatcaacaa caccacaatg tcatcttgcc agacgaagtc ttgaaagcag
    14821 cggtggatta ttctgttcaa tacattcctc aacgtagctt gccagataag gctattgacc
    14881 ttgtcgatgt aacggctgct cacttggcgg ctcaacatcc agtaacagat gtgcatgctg
    14941 ttgaacgaga aatcgaaacg gaaaaagaca agcaagaaaa agcagttgaa gcagaagatt
    15001 ttgaagcagc tctaaactat aaaacacgca ttgcagaatt ggaaaagaaa atcgaaaacc
    15061 acacagaaga tatgaaagtg actgcaagtg tcaacgatgt ggctgaatct gtggaacgaa
    15121 tgacaggtat cccagtatcg caaatgggag cttcagatat cgaacgtttg aaagatatgg
    15181 ctcatcgctt gcaagacaag gtgattggtc aagataaggc cgtagaagct gtagctcgtg
    15241 ctatccgtcg taaccgtgct ggttttgatg aaggaaatcg cccaatcggc agcttcctct
    15301 ttgtagggtc tactggggtt ggtaagacgg agcttgctaa gcaattggca ctcgatatgt
    15361 ttggaaccca ggatgcgatt atccgtttgg atatgtctga atacagtgac cgcacagctg
    15421 tttctaagct aattggtaca acagcaggct atgtgggtta tgatgacaat agcaatacct
    15481 taacagaacg tgttcgtcgc aatccatact ctatcattct cttggatgaa attgaaaagg
    15541 ctgatcctca agttattacc cttctcctcc aagttctaga tgatggtcgt ttgacagatg
    15601 gtcaaggaaa tacagtaaac ttcaagaata ctgtcgttat tgcgacctca aatgctggat
    15661 ttggctatga agccaatttg acagaagatg cggataaacc agaattgatg gaccgtttga
    15721 aacccttctt ccgtccagaa ttcctcaacc gctttaatgc agtcatcgag ttctcacact
    15781 tgactaagga agacctttct aagattgtag atttgatgtt ggctgaagtt aaccaaacct
    15841 tggctaagaa agacattgac ttggtagtca gtcaagcggc taaagattat atcacagaag
    15901 aaggttacga cgaagtcatg ggggttcgtc ctctccgtcg cgtggttgaa caagaaattc
    15961 gtgataaggt gacagacttc cacttggatc atttagatgc taaacatctg gaagcagata
    16021 tggaagatgg cgttttggtt attcgtgaga aagcctaaga cagagttttg aggataaaaa
    16081 agaaggagcc agctgaaaaa aactggttcc ttttgaggta cgacgggcat gtcgtacagt
    16141 aggttgaaac tagaatagta cacatctact tctaaaatat tgttagaaat cgatttgact
    16201 gacctgattg atttgtcctg ttcttatttc attttactat atatctgagg tgtaagtcct
    16261 cggtgggtac ctgctaccgg tgctcccaat agtgattccc aagcctgact atcgtgaggt
    16321 agcggattaa aacggtctgg ggatagaccg ttttaagtct gacgctggaa ataagaatcg
    16381 tcagaggaag ggatagcgaa atcgtggctc tacgaatagg aacgtgataa taaggcgtat
    16441 atagcggata aggggcctca aactctaaag ttcaaaaagg tagtcgtagc ctatatgcgt
    16501 aaatcacgag agcaactgaa tttgtctgtt cgaggatgga taaactattt ctcattggga
    16561 aatatgaaaa gtatagtcac tagcatagat gagcgcttgc gtactcgcct acgagtgatt
    16621 atctggaagc aatggaagaa gaaatctaga cgattatgga gattgcttaa gttaggggtt
    16681 cctaaatgga tagcagataa ggtatctggc tggggtgacc actatcaatt agtagctcag
    16741 aagtcggtac ttaaacgtgc tatatcaaaa ccagtccttg aaaaacgtgg actggtttcg
    16801 tgtttggatt attaccttga acggcatgcg ttaaaagtta gttgaaccgc cgtatgccga
    16861 acggcacgtg cggtggtgtg agaggggcta gagattatcc cctactcgat tttaaatcac
    16921 atgacgttca aaggcatcat ctgaaatccc ttgttccaag atgagttttg cccattcttt
    16981 agcagagaag aggctgtggt ccttgtagtt tccgcaagat tcgatggttg tccctgggac
    17041 atcttcccaa gtagtagttt cagcgatttc cttgagcgaa tccttgataa cagctgcgat
    17101 tttagcactg gtgtgacgtc cccacataat catgtggaag cctgtgcggc aaccaaatgg
    17161 tgaacagtca atcatgccgt caatgcgggt acggatgagt ttggctaaga ggtgctcgat
    17221 agtgtgaagg ccggcagtag ggatagagtc ttcgtttggc tgcaccaagc gaatatcata
    17281 attggagatg atgtctcctt ttggtcctgt ttcttcccca atcaagcgaa catagggtgc
    17341 tttgacaatg gtgtggtcaa gttcaaaact ttcgacaata acttcttttg acatggtaaa
    17401 tcctttcagt tttcttctct cattatatca taaaggttgt tcctgagaca gagagaaaac
    17461 ctctccgagg ctagagaggt tgaaatcttt acttacgata taagcggtcg tattggtagt
    17521 atgggtcaaa ggttacgttg atacccagtt tacgaaggac attcttgtct tcatcagtca
    17581 agatgatggt tgagtgggct tcgcttcctt tgaggttgcc gagttcttcc atagcgcggg
    17641 cagcatcagg attttctgta gctgtgatag caagtgcaat caggatttca tttgaatgaa
    17701 ggcgtggatt gcggctaccg agatgatcga ttttaagacc ttggattggc ttaacaactt
    17761 caggctcgat tagttttact tctttagcga tgtcagctga ttttttgatg gcgttgatca
    17821 aggcagcggc tgtaggccca aagagttctg agttcttacc agtgacgatt tccccatttg
    17881 gcaattcaaa ggctagggct ggtccaccag tttcttctgc tttttggcgc gcaacgacag
    17941 caaccttacg gtctgcaggt gtgataccga ggtcgttcat gagcaactca attttcttga
    18001 cggcagcttc gccaactttt tcagctttga agtcaagaat tgtttgatag taacggcgga
    18061 tgatttcttg tttagaagct tcgacagcag cctcgttatc tgtaatagcg aaaccaacca
    18121 tgttgacacc catatctgtc ggtgaagcgt atggtgattt tccgagaata cgttccaaca
    18181 tgcgcttgag cactgggaag atttcgatat cacggttgta gttgacagtg gtttctccat
    18241 aggtttgaag atggaagggg tcaatcatgt tgacatcatc aaggtcagct gtggcagctt
    18301 cataagccaa gttaactgga tgatgaaggg gaagattcca aacagggaag gtttcaaatt
    18361 tagcgtagcc agacttgatg ccattgattt ggtcgtggta catattggac atacacgttg
    18421 ccaattttcc agaaccgggt ccaggagcgg ttacgacaat caagttgcga ctggttttga
    18481 tgtagtcgtt tttgcccatg ccttctgggg aaatgatgtg atccatatcc gtcggatatc
    18541 ctttgattgg ataatgaaga taagaatcaa ttccgttttt ctcaagttga ttgcggaagg
    18601 catctgcagc gggttggcca gcgtattgtg taatgacaac ggaaccaaca aaaatcccta
    18661 attcattgaa tttgtcaatc aaacgaagaa cttcttggtc ataagaaatg cctaagtcgc
    18721 cacgtgcttt ggaatgttca atgttgctag cattaatggc aatcacaacc tcaacctgct
    18781 ctttcaattc ttgcaagagc ttgattttgt tgtcaggttc ataaccagga aggacacgag
    18841 cagcgtggaa atcttctaac attttaccgc caaactctaa gtagagcttg ccgtcaaatt
    18901 ggttaatgcg ctccaaaata tggtcgcgtt gtaaattcaa atactgttca gaactaaaag
    18961 cttgtttttt cattttttta cctctggact ctattataat aaaaaattgg aagttaggaa
    19021 actacggagc taaaaaagaa attaaaaaga ttaagcaaac gcttgcacaa aattttaaaa
    19081 agtgctatca tagactatag attatgaaaa taatgaggta aacagatgca agaaaaatgg
    19141 tggcataatg ccgtagtcta tcaagtctat ccaaagagtt ttatggatag taatggagat
    19201 ggaattggcg atttgccagg aattaccagt aagttggact atctagccaa gctaggaatc
    19261 acagcaattt ggctttctcc cgtttatgac agccctatgg atgataatgg ctacgatatt
    19321 gctgattatc aagcgattgc aacgattttt ggaaccatgg aggatatgga tcagctgatt
    19381 gcagaagcta agaagcgtga tatccgtatc atcatggact tggtggtcaa tcatacctcg
    19441 gatgagcatg cctggtttgt agaggcctgt gaaaatccta atagccctga gcgagactac
    19501 tatatctggc gcgatgaacc caatgaccta gattctatct ttagtgggtc tgcttgggaa
    19561 tacgatgaaa agtcaggtca atactatctc cactttttca gcaagaaaca gccggatctc
    19621 aactgggaaa atgaaaaact tcgccagaaa atttatgaga tgatgaactt ctggattgat
    19681 aaaggtattg gtggtttccg tatggatgtt attgacatga ttggcaaaat tcctgacgag
    19741 aaggtagtca ataatggtcc tatgctccat ccctatctca aggaaatgaa tcaggcgacc
    19801 tttggagata aggatctctt gacagtaggg gagacttggg gagcaacgcc agagattgcc
    19861 aagctctact ctgatccaaa ggggcaagaa ttgtctatgg tcttccagtt tgaacatatc
    19921 ggtcttcagt atcaggaagg tcagcctaaa tggcactatc aaaaagagct gaatatcgct
    19981 aagttaaaag aaatcttcaa caaatggcag acagagttag gagttgagga cggctggaat
    20041 tccctcttct ggaacaacca tgacctccct cgtattgtct caatatgggg aaatgaccaa
    20101 gaataccgcg aaaaatctgc caaagccttt gcaatcttgc ttcatcttat gagaggaact
    20161 ccttatatct accaaggtga ggagattggg atgaccaact atccgtttga aacactagat
    20221 caagtagaag atattgaatc tctcaactat gcgcgtgagg ctcttgaaaa aggtgttccg
    20281 attgaagaaa tcatggacag tatccgtgtt attggacgtg acaatgcccg tacccctatg
    20341 caatgggacg agagcaaaaa cgctggtttc tcaacaggtc aaccttggtt ggcggttaat
    20401 ccaaattacg agatgatcaa cgtccaagaa gcgctggcaa atccagattc tattttctat
    20461 acctatcaga aactggtcca aattcgcaag gagaatagct ggctaattcg agctgacttt
    20521 gaattgcttg atacggctga taaggtcttt gcttatatac gtaaggatgg cgaccgtcgc
    20581 ttcctagttg tggctaactt gtctaatgaa gagcaagact tgacagtaga aggaaaagtc
    20641 aaatctgtct tgattgaaaa caccctagct caagaagtct ttgaaaaaca aatcttagtt
    20701 ccatgggatg ctttctgtgt ggaattacta taaatatttt ttgcagaaaa atttaaaatt
    20761 gaaatcgtat aaaaacaagg gaggactgta taaaagacag aaatcctttg ttttttataa
    20821 ccaaggttta taaactttca ttctcgaaat tcaattaact ttacaaattc ccactattaa
    20881 ggagaaagaa aatgaacata aagaagcgtg tccttagtgc aggcctgact tttgcatctg
    20941 ctttgctttt agctgcttgc ggccaatcag gttcagatac aaaaacttac tcatcaacct
    21001 ttggtggaaa tccaactaca tttaactatc tattagacta ttacgctgat aacatagtca
    21061 attgaaacaa gaacaggaca aaagagcctc gtaaaaggta ttgcaactgg gtaatacctt
    21121 tttgaggtgc tttttgatat gagcccatgt tttctcaata ggattgtact caggtgagta
    21181 gggaggaaga ggtaaaagtt tacgcccaaa ctcctcgcat aaaagttcta acttacccat
    21241 tctatggaat cttacattat ccataataat aaccgatggt gtgtttaatg ttggtaagag
    21301 aaaattctga aaccaagctt caaaaaagtc gctcgtcatc gtctcttcgt aagtcattgg
    21361 agcgattaat tcaccatttg ttagacctgc aaccaaagaa atcctctgat atcttcttcc
    21421 agatactttg cctcttctta actgaccttt taatgagcga ccatattctc gataaaaata
    21481 agtatcgaat cctgtttcgt caatctaaac aggtactagg tgctttaaac tattaaaatt
    21541 cttaagaaat aaggctactt tttctggctc ttgttcatag taggtgtggt tctttttttc
    21601 gagtgtagcc catagctttg agcgcatagt ggatggtagt tggatgacag ccaaattcag
    21661 aagctatttc agtcaaataa gcgtctggat tgtcagtaag atagttttta agtctatctc
    21721 tatcaacttt tcttggtttt gttcctttta cttggtggct tagctctcct gttttctctt
    21781 ttagctttaa ccagccataa atagtattac gtgagatttg gaaaacgtgt gatgcttctg
    21841 ttatactacc tgttcgctca caataagaga gaactttttt acgaaaatct attgaatatg
    21901 ccataaaaag attatgccac attgtgtact atagtagatt gaaactagaa tagtacacct
    21961 ctgcttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttctt
    22021 atttcatttt actatatttt tgtttcgcgg gaagtctact aagatattta aagatgcaga
    22081 tagtaaaaaa agatgtagac attaccgtaa aaaagtgata taatcgtatg atgttcaatg
    22141 tataggtgtt aatcatgagt agacgtttta aaaaatcacg ttcacagaaa gtgaagcgaa
    22201 gtgttaatat cgttttgctg actatttatt tattgttagt ttgtttttta ttgttcttaa
    22261 tctttaagta caatatcctt gcttttagat atcttaatct agtggtaact gcgttagtcc
    22321 tactagttgc cttggtaggg ctactcttga ttatctataa aaaagctgaa aagtttacta
    22381 tttttctgtt ggtgttctct atccttgcca gctctgtgtc gctctttgca gtacagcagt
    22441 ttgttggact gaccaatcgt ttaaatgcga cttcgaatta ctcagaatat tcaatcagtg
    22501 tcgctgtttt agcagatagt gagatcgaaa atgttacgca actgacgagt gtgacagcac
    22561 cgactgggac tgataacgaa aatattcaaa aactactagc tgatatcaag tcaagtcaga
    22621 ataccgattt gacggtcaac cagagttcgt cttacttggc agcttacaag agtttgattg
    22681 caggggagac taaggccatt gtcctaaata gtgtctttga aaatatcatc gagtcagagt
    22741 atccagacta cgcatcgaag ataaagaaga tttataccaa gggattcact aaaaaagtag
    22801 aagctcctaa gacgtctaag aatcagtctt tcaatatcta tgttagtgga attgacacct
    22861 atggtcctat tagttcggtg tcgcgatcag atgtcaatat cctgatgact gtcaatcgag
    22921 ataccaagaa aatcctcttg accacaacgc cacgtgatgc ctatgtacca atcgcagatg
    22981 gtggaaataa tcaaaaagat aaattaactc atgcgggcat ttatggagtt gattcgtcca
    23041 ttcataccct agagaacctt tatggtgtag atattaatta ttatgttcgt ttgaatttta
    23101 cctcattttt gaaattgatt gacttattgg gaggggtaga tgttcataat gatcaagagt
    23161 tttcagctct acatgggaag ttccatttcc cagtagggaa tgtccatcta gactctgagc
    23221 aggctctagg ttttgtacgt gaacgctact cactagccga tggagaccgt gaccgtggtc
    23281 gcaaccaaca aaaggtgatt gtggctatcc ttcaaaaatt aacgtcaacc gaagcactga
    23341 aaaattatag tacgatcatt aatagcttgc aagattctat ccaaacaaat atgccacttg
    23401 agactatgat aaatttggtc aatgctcagt tagaaagtgg agggaattat aaagtaaatt
    23461 ctcaagattt aaaaggtaca ggtcggacgg atcttccttc ttatgcaatg ccagacagta
    23521 acctctatgt gctggaaata gatgatagta gtttagctgt agttaaagca gctatacagg
    23581 atgtgatgga gggtagatga aatgatagac atccattcgc atatcgtttt tgatgtagat
    23641 gacggtccca aatcaagaga ggaaagcaag gctctcttgg cagaatccta cagacagggg
    23701 gtgcgaacca ttgtctctac ctctcaccgt cgtaagggca tgtttgaaac tccggaagag
    23761 aagatagcag aaaactttct tcaggttcgg gaaatagcta aggaagtggc gagtgacttg
    23821 atcattgctt acggggctga aatttactac acaccagatg ttctggataa gctggaaaaa
    23881 aagcggattc cgaccctcaa tgatagtcgt tatgccttga tagagtttag tatgaacact
    23941 ccttatcgcg atattcatag cgctttgagc aagatcttga tgttgggaat tactccagtc
    24001 attgcccaca ttgagcgcta tgatgctctt gaaaataatg aaaaacgcgt tcgagaactg
    24061 atcgataagg gctgttacac gcaagtaaat agttcacata tcctcaaatc taaacttttt
    24121 ggcgaacgtt ataaattcat gaaaaaaaga gctcagtatt ttttagagca ggatttggtc
    24181 catgtcattg caagtgatat gcacaatcta gatggtagac ctcctcatat ggcagaagca
    24241 tatgaccttg ttacccaaaa atacggagaa gcgaaggctc aggaactttt tatagacaat
    24301 cctcgaaaaa ttgtaatgga tcaactaatt taggagaaat gatgaaagaa caaaacacga
    24361 tagaaatcga tgtatttcaa ttattaaaaa ccttgtggaa acgcaggcta atgattttat
    24421 tagtggcact tgtgacaggt gcgggggctt ttgcatatag cacttttgtt gttaagccag
    24481 aatatacgag taccacgcga atttacgtag tgaatcgcaa tcaagaagac aagccggggc
    24541 tgacaaatca ggatttgcag gcaggaactt acctggtaaa agactaccgt gagattatcc
    24601 tttcgcatga tgtattggaa aaagtagcga caaatttgaa gttggatatg ccagcaagag
    24661 cgttagccag caaagttcaa gtgactgtac cagctgacac tcgtatcgtc tcaatctctg
    24721 tcaaggataa acagccagag gaagccagtc gcatcgctaa ttctctacga gaagttgctg
    24781 cagaaaagat cgtcgctgta acgcgagtat ctgatgtaac gacacttgaa gaagcgcgac
    24841 cagctacgac tccctcttct ccaaatgttc gacgcaattc cttgtttagt tttcttggag
    24901 gagcagtcgt aacagtaatt gctgttcttt tgattgagtt gctcgacact cgtgtgaaac
    24961 gtcctgaaga tgtcgaagat gtactgcgaa ttccactttt agggctcgtt ccagatttgg
    25021 acaaaatgaa ataggaggaa gttatgccaa cgttagaaat ctcacaggca aaattggatt
    25081 ttgtaaaaaa ggcagaggaa tattataatg ctttgtgcac gaacctacag ttaagtggag
    25141 ataatttaaa agtattttct atcacttctg tgaaactagg agaaggaaaa tcaacgactt
    25201 ccaccaatat cgcttgggct tttgcgcgtg caggttacaa aacgctgctg attgatggag
    25261 atattcgcaa ttctgttatg tcaggtgtct ttaaagcaag ggataagatt acaggcctga
    25321 cagaattttt atcaggaact acagacctat cacaagggct ttgtgatacc aatatcgaaa
    25381 atctctttgt aattcaggct ggttctgtgt caccgaatcc gacagctctt cttcaaagta
    25441 agaatttcag tacaatgctt gaaaccttgc gtaaatattt tgactacatc attgtagata
    25501 ctgctcctgt cggtgtcgtg attgatgcag ctattattac gcgaaaatgc gatgcttcta
    25561 ttttagtgac ggaggcaggt gaaataaatc gacgggatat tcaaaaagca aaagaacagt
    25621 tggaacacac agggaagccg tttttgggag ttgtgttgaa taaattcgat acttcagtag
    25681 acaaatatgg ttcttatgga aattatggag attacgggaa aaataaaaaa taggtcgggg
    25741 ggatagagat gaatggaaaa atagtaaagt cttcattggt cataatccag agttttcttg
    25801 ttattttatt gacttatcta cttagtgctg tgagagaagc ggagattgtt tcaacaacag
    25861 ctattgcact ttatatcctc cattattttg tcttttatat cagtgattat ggacaggctt
    25921 tctttaaaag gggatatttg attgaacttg tccagacatt gaaatatatc ctattctttg
    25981 cactagcgat tggtatttct aattttttct tagaggatcg atttagtatt tccagacgag
    26041 gcatgattta cttcctcaca ttacatgctc tcttagtcta tgtgctaaac ctatttatca
    26101 agtggtattg gaagcgggct tatcccaact ttaaaggaag taagaagatt ctcctactta
    26161 cagcaacttc tcgtgtcgaa aaggtactgg atagattaat agaatcaaat gaggttgttg
    26221 gggagttggt agccgtcagt gtcttagata aaccagattt tcagcatgat tgtttaaagg
    26281 tagtagcaga gggggagata gtaaactttg cgactcatga ggtggtcgat gaagtcttta
    26341 tcaatcttcc aagtgaaaaa tacaatattg gagagcttgt ctctcagttt gaaacgatgg
    26401 gaattgatgc aacagttaat ctaaatgcct ttgatcgtag tttggcacgt aacaagcaaa
    26461 ttcgtgagat ggcaggatta aacgttgtga ctttttctac aacattttat aagactagtc
    26521 atgtaattgc taagcggatt attgatatcg tgggtgcatt ggtagggctg atactatgtg
    26581 gtctagtcag tgttgtactg gttcctttga ttcgaaagga tgggggctct gctatttttg
    26641 ctcagacgcg tattggaaaa aatggtcgct acttcacttt ttacaagttc cgctccatgt
    26701 gtgtagatgc cgaggcgaaa aaaagagaac tcatggaaca aaataccatg cagggtggaa
    26761 tgtttaaggt ggacgatgat ccgcgtatca cgaaaattgg tcgttttata aggaagacta
    26821 gcttggacga gttgccacag ttttataatg ttttaaaggg agatatgagt ttggttggca
    26881 cacggccacc aacagtggac gaatatgagc actatacccc agaacaaaaa cgtcgtctaa
    26941 gttttaaacc tggtataaca ggcttatggc aggtcagtgg acgaagtgaa atcaaaaatt
    27001 tcgatgaagt tgtcaaatta gatgtggctt atatagatga ttggacaatt tggaaagaca
    27061 ttgaaatttt attgaagaca gttaaagtag tatttatgag agatggagcg aaatagttta
    27121 cttttgtttc agactactag gagaaaaatg aagaagtcag tttatatcat aggttcaaag
    27181 ggaattcctg ccaagtatgg aggatttgaa acctttgttg agaaattaac agaatatcaa
    27241 aaagatggta acatccaata ctatgttacc tgcatgcgcg aaaattcggc aaagtcagga
    27301 tttacagcag atacatttga gtacaacggt gctatttgtt acaacattga tgtgcctaat
    27361 attggtcctg ctagagccat tgcctacgac attgcagcgg tcaataaggc tattgaattg
    27421 gctaaagaaa acaaggacga ggctcccatt ttttacattt tagcttgtcg tatcgggcct
    27481 tttatttctg gacttaagaa aaaaattcgt tcgatcggag gccgtttgct ggtaaatcca
    27541 gatggtcatg agtggcttcg agctaaatgg agcttgccag ttcggaagta ttggaaattt
    27601 tcggaacagt tgatggttaa acatgcagat ttattagtct gcgatagcaa aaatatcgaa
    27661 aaatatattc gagaggacta taaacagtat cagcccaaga cgacctatat cgcttatggt
    27721 acagatacta ccccttcaag tctgaaatca gaagatgcca aagttcgaaa ctggtatcgt
    27781 gaaaagggag taagcgaaaa tggctattat ctagtggtgg gacgattcgt tcccgaaaac
    27841 aactatgaaa ccatgattcg tgaatttatc aagtctaagt ccaataagga ctttgtcctt
    27901 attacaaatg tggaacagaa taaattttac gatcagttgc tcaaggaaac aggtttcgac
    27961 aaggatctaa gagtcaaatt tgtgggaaca gtctatgacc aagaattgct caagtatatc
    28021 cgtgaaaatg ctttcgccta tttccatgga catgaagttg ggggtaccaa tccatcgctt
    28081 cttgaagctt tagcatccac taaactcaac ttgttgttgg atgtcggctt taaccgtgaa
    28141 gttggtgaag acggagccat ttattggaaa aaagatgagc tagcgcatgt aatcgaagaa
    28201 gtggaacgat ttgatgaagg tgatatttcg gaattagacg aaaagtctag ccaacgaatt
    28261 gcggatgcat ttacgtggga aaagattgtg tcagattatg aagaagtgtt taaaggataa
    28321 ggaatgaaac ggattttata cttgcatgct ggtgcagaaa tgtatggagc agataaggtt
    28381 ttattggagt tgatcaaagg attggattcc aaagaatttg agacgcacgt tatcttgcca
    28441 aatgacggtg ttttagtcga ggctttgcgc caggttggag ctcaggtcag cgtgttggat
    28501 tatccgattt tacgtagaaa atactttaat cctaagggga tttttgaata tctgaaatcc
    28561 tatcatcgct actctcaaaa aatcgctcaa tatgtgcgtg agaatgggat agttcttgtt
    28621 cataacaata caacagccgt tctggaaggg atttatctaa agagaaaagt aaaacttccc
    28681 ttgatctggc atgttcatga gattattgtt aagcctaagg ccatttcaga ttttatcaac
    28741 ttcttgatgg gacgttatgc aaataaaatc gtaacagtat ctcaagcagt ggccagtcat
    28801 gtcaatcagt ctcccttcat caaagaaggg caggtccagg ttatctataa tggagtggac
    28861 aatgcggtct atcatccgat gccagcaagc acagtgcgtg aacaattttc tatcccggaa
    28921 gaggctcttg tcattggtat ggttggtcgc gtcaacgctt ggaaggggca aggagatttt
    28981 ctagaagcgg tgacgccgat tttggagcag aatcccaatg ccatcgcttt tctggctgga
    29041 agtgcctttg ctggtgaaga gtggcgagtt gaagaactcg agtctaagat tgccaaatca
    29101 agtgtagtct cacaaatcaa acgaattgac tattatgagc atacgactga gctttataat
    29161 atgtttgata tctttgtttt accaagtacc aatcctgatc ccctaccaac agtggtacta
    29221 gaggcgatgg catgcggcaa accagtagtc ggctatcgtc atggtggtgt ttgcgaaatg
    29281 gttgatgaag ggacaaatgg cttactggcc actccaaatc agcctgctga actatcgaaa
    29341 gtcattcaag aattggtgga gaatccagag aaaagaaacc aatttggcca agcatcagta
    29401 gaaagacaaa gagagttgtt ttctttagat agttacatta agaacttctc ggagttgtat
    29461 aaaacagata gaaaagatta gttatgaata aaatagattt tgttgttact tgggttgatg
    29521 ggaatgaccc tgtatggaaa gaaaaaaaat caaggtatga tggctcggtc agtacttcta
    29581 aacagagtat gaactcggtc aaagcttacc gagaatgggg aacctttaaa tactggttta
    29641 gaggagttga aagatttgcc ccttgggtca ataaggtcta cctcgtgacc gaccagcaaa
    29701 gaccaagctg gttagatata aacagtgaaa aattggtctt ggtagatcat acagagatta
    29761 tctgtaatga ctgcctacca gttttttctg ccaatccgat cgaaagtaat atccatcgaa
    29821 ttcctggtct ctctgagcac tttgtctttt ttaatgacga tatgtatttg acggctccag
    29881 ttgaaccgac agattttttc tcagaggatg gtttacccaa atataatacg gctctttctc
    29941 cgattattcc tgaaagatat ggaacaggaa attttcagat aaatgacatg gaaattgtca
    30001 ctagctattt cagtcgaaat gaaattttga agaatgggca attctttgat ccaaagcagg
    30061 gattaaaaag tattgttaag tcgctactat acagaaatag tcagtttatc tgtggtttct
    30121 gggagagtca tctgccctat cctctactaa aatcaacgat ggatttgata tgggaaaagg
    30181 aaaaagctgt cttggggaga acctcagcga gtcgctttag aaatccgtct gataccaatg
    30241 tttggctctt taagtattgg cagattgcta gtggtcaata cgctgtcgga aatcctaagt
    30301 tggggagact cttttctttg gataatgccg gaccagattt ttggaactta ctaaattctg
    30361 gcaaatacaa aattatgtgt atcaatgatg tgtatgatat taaggatgaa gaaaaagtaa
    30421 tgcaagaatt tattgctgca atgaaaaatc ttttgcctga taaaagtaca tttgaattat
    30481 aaaaaaggag tgggaatatg ccatcagttt gtgctttagt tgtgacatat aatcgttcaa
    30541 aatatctgca aaaggctttg gaaggtattt tgaatcaaca acaagaaatt tcgggaattt
    30601 taatttataa caataatgct acagataata ctgaagaaat tcttatgaaa ttcggttatg
    30661 ttgattcaaa aggcgataag ataaaagaaa attatcttta ttcgaccgaa aaagatggaa
    30721 aacacttcta ctattaccat aatgacgaaa acctaggagg agctggaggc tttgcaaatg
    30781 gaattcgatt aatttcagag cttgattatg attatgtttg gattatggac gatgatgtct
    30841 atccggaacc taattgttta tccgaaatta tgaaacaaat gagtgtccaa aatgtacagg
    30901 taggtatccc gaatcgtaca gatgaaaatt ttgatgaccg tgccattatc ggttttgatt
    30961 ttgatgacta tcataaattt tggacggaaa tgagaaaaac agtaacatac ggtccctttg
    31021 atgaagaagc gataaaagtg gttgatatgc catttgaagg accagttgtt gaaatggcat
    31081 tgcttcgtaa agttggtatt cctgatagtg gcttttttat tgagtacgat gattcggact
    31141 ttgcacaacg cctacaacag tattcagata taatttttgc aacaaaagca caattgcatc
    31201 gtcaacttgc tgtaaaagtg gatccaagcg aggtaaaaaa agtggaacct tataattgga
    31261 gaaattacta taaaattcga aataatatta ttttcgataa gcgatatgga aaaaattgga
    31321 aagtacgtca attgagtcct ttaattctaa ttgctcacca tattgtgatt gctattcgcc
    31381 atcagcattt aaaacataac ttgccaatta tttggaaggg tttttgggat ggagtttttc
    31441 aacgaatggg gaaacgagta gaccccaatt attaaaagga gaaaaaaatg tctgatttac
    31501 tgttgagttt cattatgccg acctataact ctgcagaata cttagcagat acaatggata
    31561 gtttagtagc ctctataggt gattataatc atttagtgga aattatttgt gttgacgatg
    31621 gctcatggga taatacagtt actattttag aaggatacaa gtctatttat gataatttga
    31681 ttattataag aaatgagcat ggtggggtta gtcaggctag aaatacagct ttagatatag
    31741 tttctgggaa atatatcagt tttgttgata gtgatgattt gtatgagaaa gattttttaa
    31801 atacttttat ttcattggac aagaattttg atttcctttt ca
//
LOCUS       DAVKHZ010000016        28975 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000016 DAVKHZ010000000
VERSION     DAVKHZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.016"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(248..1855)
                     /locus_tag="VWW99_000688"
     CDS             complement(248..1855)
                     /locus_tag="VWW99_000688"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9538565.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2245..2832)
                     /locus_tag="VWW99_000689"
     CDS             complement(2245..2832)
                     /locus_tag="VWW99_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9538566.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2960..3271)
                     /locus_tag="VWW99_000690"
                     /pseudo
     CDS             complement(2960..3271)
                     /locus_tag="VWW99_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3169..3585)
                     /locus_tag="VWW99_000691"
     CDS             complement(3169..3585)
                     /locus_tag="VWW99_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538567.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            complement(3612..4238)
                     /locus_tag="VWW99_000692"
     CDS             complement(3612..4238)
                     /locus_tag="VWW99_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9538568.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4252..4815)
                     /locus_tag="VWW99_000693"
     CDS             complement(4252..4815)
                     /locus_tag="VWW99_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9538569.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(4980..5214)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5268..5771)
                     /locus_tag="VWW99_000694"
     CDS             complement(5268..5771)
                     /locus_tag="VWW99_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9538570.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(5964..6794)
                     /locus_tag="VWW99_000695"
     CDS             complement(5964..6794)
                     /locus_tag="VWW99_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9538571.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRLLV
                     LIAIFSVFLLYLSEIHFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGAYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6779..8461)
                     /locus_tag="VWW99_000696"
     CDS             complement(6779..8461)
                     /locus_tag="VWW99_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538572.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKKEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEEQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTDPASLLSNPELLVKANLKETSIFNLAKKLDVDPLALTAFYKERREGCKLN"
     gene            complement(8563..8775)
                     /locus_tag="VWW99_000697"
     CDS             complement(8563..8775)
                     /locus_tag="VWW99_000697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538573.1"
                     /translation="MKIKEQTRKQVAGCSKQCFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(8782..9330)
                     /locus_tag="VWW99_000698"
     CDS             complement(8782..9330)
                     /locus_tag="VWW99_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9538574.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGVIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9345..10193)
                     /locus_tag="VWW99_000699"
     CDS             complement(9345..10193)
                     /locus_tag="VWW99_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9538575.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10382..11731
                     /gene="trkA"
                     /locus_tag="VWW99_000700"
     CDS             10382..11731
                     /gene="trkA"
                     /locus_tag="VWW99_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9538576.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11735..13174
                     /locus_tag="VWW99_000701"
     CDS             11735..13174
                     /locus_tag="VWW99_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9538577.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSEQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLVYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13300..14979)
                     /locus_tag="VWW99_000702"
     CDS             complement(13300..14979)
                     /locus_tag="VWW99_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9538578.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNESLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(15049..16461)
                     /gene="lacG"
                     /locus_tag="VWW99_000703"
     CDS             complement(15049..16461)
                     /gene="lacG"
                     /locus_tag="VWW99_000703"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9538579.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(16471..16815)
                     /locus_tag="VWW99_000704"
     CDS             complement(16471..16815)
                     /locus_tag="VWW99_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9538580.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(16983..18869)
                     /locus_tag="VWW99_000705"
     CDS             complement(16983..18869)
                     /locus_tag="VWW99_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9538581.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(18871..20193)
                     /locus_tag="VWW99_000706"
     CDS             complement(18871..20193)
                     /locus_tag="VWW99_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9538582.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            20361..21584
                     /locus_tag="VWW99_000707"
     CDS             20361..21584
                     /locus_tag="VWW99_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9538583.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            22236..22406
                     /locus_tag="VWW99_000708"
     CDS             22236..22406
                     /locus_tag="VWW99_000708"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538584.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            23018..23302
                     /locus_tag="VWW99_000709"
     CDS             23018..23302
                     /locus_tag="VWW99_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538585.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            23295..23543
                     /locus_tag="VWW99_000710"
     CDS             23295..23543
                     /locus_tag="VWW99_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538586.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            23639..23857
                     /locus_tag="VWW99_000711"
     CDS             23639..23857
                     /locus_tag="VWW99_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538587.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(24225..26549)
                     /gene="pflB"
                     /locus_tag="VWW99_000712"
     CDS             complement(24225..26549)
                     /gene="pflB"
                     /locus_tag="VWW99_000712"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9538588.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            26898..27959
                     /gene="dinB"
                     /locus_tag="VWW99_000713"
     CDS             26898..27959
                     /gene="dinB"
                     /locus_tag="VWW99_000713"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9538589.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSN
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(28106..28951)
                     /locus_tag="VWW99_000714"
     CDS             complement(28106..28951)
                     /locus_tag="VWW99_000714"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9538590.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGIAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT         9148 a         6137 c         5045 g         8645 t
ORIGIN      
        1 tttcaattca ctatacaatc cagttcctaa aactttgcta gaaattgatt tggctttccg
       61 aattaatttg ttcaaatttt atttcatttc aatatactga taaatccatt cactagcaca
      121 gggcaagagg gagtcgctat tgatttcaac ggtagcaggt acatacagag gactcccacc
      181 tcagatatac tgcaatatca tcgtatgcaa aaaacctctg agatttttct caaaggttct
      241 gattttgcta attgctgttc tcaaccgctg cagtaacaaa ggcagtgtag agttcttctg
      301 gtcggtttgg acggcttgac agttcagggt gatactgaca agctacaaag aatttatttt
      361 caggaatttc cacgatttct accaaacgat tgtctggaga aactcctgaa aagacaaaac
      421 ctgctgcctc aaactgctca cggaaggcat tattaaactc ataacggtga cggtgacggc
      481 gttgcaccac ttcttgattg tgataagcag cagcagcctt agagccacgt ttcaacttag
      541 acggataaag tcccaaacga agggttccac ccatatcctc aatatcaatc tgatcacgca
      601 tgatatcaat gatagggtat tttgtttctg gtgcaagctc tgcagaattg gcaccttcaa
      661 gacctaaaac gtgacgagca aactcgatac atgtcaactg cattcccaag cagactccca
      721 acattggaac atcattttca cgcgcatagc ggatggcttg gattttccct tctgtaccac
      781 gttgaccaaa accacctggt acgatgatcc cgtccgcatc agacaagagt tctgctacat
      841 tctctgctgt cacatcattg gcattgaccc aattgatttt aacttctaca tcattgacat
      901 agccagagtg tttcaaggct tcgaccactg agatataggc atcttgcaac tccacatact
      961 taccaacaag ggaaatctta acttgtttct tgaggttcat gaccttgtcc accatggctg
     1021 accattctgt catatccgct gctggtgcgt ctaatttcaa atgatcacaa acaatttggt
     1081 ccatcccttg tgcctgcaag ttcagtggaa tttggtaaag gtgttcaaca tccaacgatt
     1141 cgataacggc ttctggtgcc acatcacaga actgggccag tttattttta attccttgac
     1201 cagctggctc ttctgtacga ataaccaaca tatttggttg gattcccaag ccacgcaatt
     1261 ctttgacaga gtgttgggtt ggtttggttt tcatttcacc agcagccttg aggtaaggaa
     1321 gcaaggttgt atggatatac atgacattat ccgcacccac atctgccttc atctgacgaa
     1381 gagcctctag gaatggcaag gactcgatat ctcctactgt tccaccaacc tctgtgataa
     1441 tgacatcaga gtcggtcgtt agagcggcac gcttgatttt ttctttcaaa gcatctgtga
     1501 tatgaggaat gacttgaaca gttgccccaa ggtattctcc acggcgttct ttacgaagaa
     1561 cttcactgta aattttccca gttgtcacgt tggaatattt gttgagattg atatcgataa
     1621 aacgttcata gtgacccaag tccaaatctg tctcagctcc gtcatctgtc acaaaaactt
     1681 ccccgtgctg gtaaggactc atggttcccg gatcaatatt gatataaggg tcaaactttt
     1741 gaatggttac tttgagacca cgatttttca agagacggcc tagactcgct gccacaatcc
     1801 ctttcccaat agacgatacc acaccaccag ttacaaaaat atatttcgta gacatagatt
     1861 cctctttcta aaatgctcaa ggtcttgtta actacgaggg gatatagaaa acaagaccct
     1921 actaataaga ataatctggg acgactgcac cagattcaca actaatactc ttcgaaaatc
     1981 tcttcaaacc gcgtcaacgt cgccttgccg tagatatgtg ttcctgactt tgtcagtctt
     2041 atctacaacc tcaaaacagt gttttgagca gcctacggct agtttcctag cttgctcttt
     2101 gattttcatt gagtataaaa tacaagaaga taacttcttg aaaaaacaaa aatagctccc
     2161 taagaactag ggagccccga cctctaaaag aggtgcccaa gcaatatgat acctaaaaat
     2221 aggataattg tcaatagcta actcttattc ctcgctggtt tcagcatcgt catctgaaaa
     2281 ctcgtcgtcg tcttcgttga gatccacatc ttctcccaag tcatctggag cgatttcgtt
     2341 gatttctgca tcataagctt ccacttcatt tttttcatca tctggatttt catcatcgta
     2401 tgaaagagct ggatctgctt cgtatgcatc ttcgtcttct ggatcatctg cattgtagtc
     2461 aatggcatct gaatcaccat ccataaaggc attgacacgt tttttcttag cttttggtgc
     2521 tacttcatcg tcgtcatttt cttcaagagc gatgatttct tcgtcgattt cgtccacacc
     2581 ataccatgaa cgaagacccc atttgttgtc cccaagtgag atgaagctac cgtcaaagtt
     2641 caactctgtg tagaacaaag gcaaagcttc gcggatatcg ctgtttgatg ttccaaggta
     2701 gttttgaatt tcgtttacaa gatcgctaaa atgcatctca tgatcgcgac cacgaagttc
     2761 caagatagca cgcgctacct caatcataga tagttcactt ttttcttgcc cagcaaatac
     2821 ttctaattcc aaagcgtttc tcctcattta tactactatc gccagagcga acagactctg
     2881 acctcatttt atcatttact ctttatttta cgataatttt gcggaatagt caaaggttaa
     2941 gggggagaaa gtggcaggat tagactaatt ccaatataaa actccttcct ttttctgttg
     3001 ctccattttc cacaaatcca agcgacttga aacacctcct agaagcatga ttgtaggtgt
     3061 agatttcctt gactctcaat tctttccatc cttttactcg agccaattca atcaaagcac
     3121 ttagaatctt ttttccaagt cctcgatgtt ggtaagcgga attcccaatc acaatgggga
     3181 gattatcctg agatagtgta atatccccaa ttggaaacca ttctcccttc tccttgactt
     3241 caatccaaaa aagctcacca tgccgatcca aagaggtaag atatgatttt ttaaatcaaa
     3301 aacagagtaa atctttcttt cctcgcaagc ttgcgcatag agatgatata gttcatcacc
     3361 accatctcta tcccactcag cagaaatcgt atcccgacct gccaataaag cctgataagc
     3421 cctgtgatgc ccatctgtaa tcagcaaaca atctccaaag gcaagaatac tgattggatc
     3481 gacttggatt gtttctgccg actggtaaag catctgaata tcttgcaact tcttttctga
     3541 taaatatagt tgagtcggat gaagatctgc tatattgact ttcatttctt tctctcaagg
     3601 gaattcgata ctcacttctg tttgccttta aatcgccatt ggaagcggag cttgtcataa
     3661 aagggaaact cgataaacag gactcccaag cccacacaga gactggcaag gacgtctgat
     3721 gggtaatgaa ctcccagata gactcttgat accagcacac tgactaggta gaggccaagg
     3781 acgatttgta cgatttttct ccagacctga tctttaatcc gctgactaag aataacaatc
     3841 aaagtcccta ccatcagcgt tacagctaga gaatgcccac ttgggaagga aaatcccttc
     3901 tcctccacca gatgtaaaat agctggtcgt gggcgctggt agatattttt aaaggtcacg
     3961 attaaaagac ctgccaaagc cagatttccc agcatgaaga aactttctat cttccatcgc
     4021 ttacgataaa agacaaaagc tgtaatgaca acccaagtga taatcactgg gatatcaatc
     4081 agacgtgtga gggctcgaaa aagaatagtc aaataatctg gtaagtctcc tcgaatggca
     4141 gtctgaatcg attggtcaaa attgaccaac atttcagggt aaaatttgac catatagcca
     4201 agaataacga aaagtaaaag ggcaaaactg cccttcatta aaaatgtttg tttatctttc
     4261 atagtgtttt aaggttggtt tcaaaaggac gtacaacaac cagaatgaaa cggaaaagat
     4321 tacaccctca atcaagttaa aaggtaatac catggtcatt aggtagttgg aaagtcccaa
     4381 aatttttcca atatcaaagt tagcaaactt agcgtacaaa ggaacagcat aaacatagtt
     4441 gagaaccaac atggccaagg ttaaaccaac agttccagct agagagccta gtaggaaacg
     4501 aagggttgtc cgttcctttt tccaaatcaa agcaaatacg atgacaaaaa ctcccaaagc
     4561 tacgatattc atcggcaaac caatgtaagt attcactcct tggctgttaa gaagcaattt
     4621 caatagtgag cgaagcaaga gcactcctag agcagcaggc aaatccatga ccaccagacc
     4681 cacaaggact ggcaagatac taaattcgat cttgaggaaa gatgccgctg gtaaaagcgg
     4741 aaagtcaaag tacatcagca caaatgagat ggctgataga attgcaatgg tcgaaagtcg
     4801 acgtgtgttt gtcataacag gttcctccaa ttttctataa aatcagaaga agttggaaag
     4861 gattcctcta tctattctca ctttttatat cccaaaagtt ccctcttact ctattaaaga
     4921 aaaacaaagc aagtggttac aatccggcta taaatctatc aaaacagaca aagctattct
     4981 ttcgtcttct cccatccaga ctatactgtc ggttgtggaa tctcaccaca tcagcttgcg
     5041 ctcgcggact tctttaaaga gaaggaaata gagatttttc ttttagacta ggcgacaagc
     5101 tgtaggcata acttctggtt aggcaaagct tgtcaacaaa gtataaaagg aaaagatcat
     5161 ttcctagtta aagtcaccgc cggtcgggaa tcacaccctg ccctgaagac tcttttatca
     5221 taacaaaaaa cgcttgcaag cgcaagcatt ttgttcattt tcatttttta tttcaattta
     5281 tccacttcat aggtatggac gagttcaaga cctgcaaagt tctgctggcg aagagcctca
     5341 tagacaatca tgcagacggt attagacaca ttgagactgc ggacatgttc atcattcatg
     5401 ggaatacgaa gagccttctc aggatgttct cgcataaaat cttcaggtaa gcccttgtct
     5461 tcacgtccaa agagaaaata atggtcctcg tcagtcgata aatccacctc agaatagatt
     5521 ttttccgcga atttcgaaat cagatagagt tttcccttca tctgagacat gaaatcttct
     5581 aagctctcgt aaaaataaat ctctagctta tcccagtaat ccaatccagc ccgcttcatc
     5641 ttgcggtcat caataggaaa gcccataggc ttgatgatgt ggaggggaga attggtcgca
     5701 gcgcaagtac gcgcgatatt gcctgtattt tgtggaattt gaggttcaaa taatacaatg
     5761 tgatttgtca tgacttgctt cctttcacca ttgcaaaaaa atagccacac tgcccggagt
     5821 caagctcagc aaacagcgtg gttaaggcat cgttaactta cctcacaaca ggtttgaagt
     5881 aaatcagcga aactactttc ttagtataac actttcagaa tcattgtcaa tagaaatgac
     5941 ttgatttttt caattttttc aagctatttc caagggttgt aaaatcgtcc ctgattctgc
     6001 aagataagta gtaaactaac tactaaaaac aaggttgcca agagcaaggt aatatagtct
     6061 ccttttttca aggcctgata actataccat gtgcgttttt tctctttccc aaagcggcga
     6121 agctccatgg cagtcgcaat ggtatcaatg cgttctagcg agctaaaaat caagggagta
     6181 ataatgagca gattgccttt gattcgttgc ataagagaag ctttcttgga taattccatc
     6241 ccacgcgctt cctgagacat cttgatggta aagaattctt cctgcaaatc tggaatatag
     6301 cgcaaggtta gactgacaga ataggcaatc ttgtaaggca caccaatttg atttaaactg
     6361 gaagcaaact gactagggtg ggttgtcatc aaaaagataa tagccagagg aatagtgcaa
     6421 aggtacttaa tagccaaatt tagcagataa aagagctctt gactggtcag agtgtaggca
     6481 ccgattccct gccaaatcac acttctctct ccgtaaagtc caaccccata ctcgggagaa
     6541 aagagataga ccatcaaaac gtttaaaacg gcaaataccg tcgcaaaaac ggctacaaag
     6601 gaaacatctt taaaatggat ttctgacaaa tagaggagaa agaccgaaaa gatggcaatc
     6661 agaacaagca gtctggtatc atagctaatc atggccgcca atgacacgag aatgaaaaag
     6721 aggagtttcc cagcgcctga caagcgatga atcacagtat ctctatgctg gtaaccgatt
     6781 aatttagctt gcatccttct ctcctttctt tgtaaaatgc cgttaaagca agtggatcca
     6841 cgtcgagttt cttagccaag ttgaagatag aagtctcttt taggttggct tttactaaca
     6901 gctcaggatt gctcaacaga ctagctggat cagtatcagc aatcaattcc ccatccacca
     6961 tgacaagggc tcgatctgaa taatccagca tcaattgcat atcatgggta atcatgacaa
     7021 tggtatgccc ttgttgatgc agttcttcga gaaattccat aatctcagta tagttcttct
     7081 ggtcttgacc cgcagtcggt tcatctagga gaataatttc agctcctaag accaaaattg
     7141 aagcaatggt gacacgtttt ttctgaccaa atgacagggc agaaataggc caattacgga
     7201 attcataaag tccacagatt ttcaaggttt catagactct cgtttcaatt tccttctcat
     7261 ccacacctcg caaacggagc cctagagcca cctcatcaaa aatcatattg gttgaaatca
     7321 tttgattagg attttgtagc acatagccta ctcgttccgc ccgctctgca acagaatcgc
     7381 cttttatatc ctgttcttcc caaagatagc gtccttccgt ctgaataaag ctacttatag
     7441 ccttggctag agttgatttc cctgctccat tttttccgac aatagcaatc ttttcaccct
     7501 ttttaatatc taaatgtagg gattttaaaa tcggtctatc atcataagag aaagacacgt
     7561 cctctaattt aaagagtgac tgcaattctg gagtttcttt tgccagttca ttctgcagct
     7621 gaacctgacc ttttgagata gacatgttat ccagattcgc taactgttct ttcttgacta
     7681 agtccacacc taattgacgg agagtcgtta gataaagggg ttctcgaatt ccattttgag
     7741 tcaataaatc agttgcaagc aactggtcag ggctcccatt aaaaaggata cgaccatcgt
     7801 ttatcaagac aatccgatcc acagggcgat gcagaacatc ctccaaacgg tgctcgataa
     7861 taagagtcgt cgtcccctct tccttatgaa tctggtcaat caattcgata atatcctgac
     7921 ctgacttggg atctagattg gcgagtggct catcaaacaa gagaatcgga ctttcatcaa
     7981 tcaagacacc agccagactg actcgctgct tttgtccacc tgacaaatcc tgagggcgct
     8041 gatccagtaa aggaagaagg tccagctttt cagcccattt ataaacacga cctttcatct
     8101 catctagggc tgtcacatca ttttccagag caaacgccaa atcttctgcc acagacaagc
     8161 caataaactg cccatctgta tcctgcaaaa ctgtgctaac cagatgagac ttatcataga
     8221 tgctcatatc aaaggctgtt tgacccttta tcaaaaattc tccatatgtc tgacccttgt
     8281 aaatattggg aataatccca ttcaaacact gacccaaggt agatttacct gatccagatg
     8341 gtccaacaat taagactttc tctcccttgt aaatggtcaa gtctatccct tgcaaggtcg
     8401 gttcttgttg tgtttcatac cggaaagaga aatccttcca ctcaattata gcttctttca
     8461 tcttactctc ttcattcgct tcttagactt ctattttatc ataaatcaag cccttcttgc
     8521 agtctcttct cttaaaatct tagcgccaaa aagattccta tcctagctta cttgcctaac
     8581 taatctataa acatcgaaaa agactagttg cccagccttc cccatcattt tatactcttc
     8641 gaaaatctct tcaaaccacg tcagcttcgc cttgccgtag gtatggttac tgacttcgtc
     8701 agtttcatct acaacctcaa aacactgttt tgagcaacct gcgacttgct tcctagtttg
     8761 ctctttgatt ttcattgagt attagtcctt tttcaaactt cctgcacgag tttgagttcc
     8821 tgcataggca agtaagagaa gagttcctgc aatagctaca gatacaccat tggcaattcc
     8881 cgcaacaatc ccttgtgcaa atactttttc tgccgcttct tgataaatca caacatctcc
     8941 aagtggtgcc aagacacccc aaacaagggc atttgcaagt agttgaatga ggttaaaaat
     9001 aagaatatct ttccagtcaa aaacaccatt gatcactcga acgtactttc taaaaagtcc
     9061 cacaactaaa ccaaagagtc cgctagcgat aatccaagtc caccatagac cataaccagc
     9121 aagagagtcc ttgattgcat gaccaatcac cccgacaagc aaaccgataa tcggtccaaa
     9181 aataatagaa agtagcgctt gtaccgcata ctgaagctgg atgcttgtat ttggaacagg
     9241 ggttggaatg ttgatcatcc cgatgacgac aaagagggca gcgccaattc cgacagcaac
     9301 aacttgttta attgtaaatt tgatttccat actattctcc tattttatcc ttctattttc
     9361 tttatttcaa tggtccaaga tgaaccgaca cctacattat aggccttggc aaaggaacct
     9421 tggttgatag ccaaacctaa acgatagaga gagttgatgt aaaggatggg ttgcccaatt
     9481 ctcacatctg caaatgattt gccatagaca acctgatttt gatagaccag catatcagca
     9541 tgatagatgg tcacttcaaa acgatcacca aattctggtt ccagcttgta aaattcttcc
     9601 cgtgtgatag aggtccaaag cgaaccgaaa cgcacatcca gaatatcaat ggctcccttc
     9661 accagatgat cttctatgat ggtcgctacg actggaagct ctacaatctg ttccacactg
     9721 agctctggcc ctacttcctc aaaagtaatg tgaccactgg ccagtttagc accagtataa
     9781 gcatagacat cacgaccgtg gaaggtataa gaatgctctg tgttttgacg cctattggcc
     9841 acctcagaaa tctcacgaat ggctacaatg ccaacgtgtt tcttgataaa ggaaagcgtc
     9901 ccattatctg gcgtgacaat gtattgattt tttgcagtct tggcaactac actcttacgt
     9961 ttcgaaccga cacctggatc gacaaccgat acaaacgtcg ttccctcagg ccagtaatcc
    10021 accgtctgaa agagacgata gctcccctca aaaatattat aaggcgtgat atcgtgcgtc
    10081 aagtgatgga tttttaaggt tggagactct tctaaagcca ctccaatcat agccgatacc
    10141 gcaccatcaa ccagaccaaa gtctgattgt aataccagta aattattatt cattttatct
    10201 ccttgtatat cctttatcat accatatttc agagaaagtc gctaatagct atttcaattg
    10261 gttagggtat agttttacct tatagcaaat ttcctactaa aagttcttgt tattagctag
    10321 taggtgcatt ttttcttgaa aaagggtatg atagttacat catgaaaaag taggttttat
    10381 tatgaaaatt atccttgtcg gagggggaaa agttggtttt gccctctgtc gctccttggt
    10441 tgcagaaaag catgatgttt tgctgattga gcaagacgaa gctgttctca atcatattgt
    10501 cagtcgcttt gatatcattg gtatccttgg taacggggcc gattttgcca ttcttgagca
    10561 agccagcgtc caagattgtg atatctttat cgccctgact gagcacgatg aagtcaacat
    10621 gattgcagca gttctagcca agaaaatggg agctaaagaa actatcgttc gggtgcggaa
    10681 ccctgaatat tctaactctt atttcaagga aaagaatatt ctcggttttt ctcttatcgt
    10741 taatcctgag ctcttggctg cccgcgctat cgcgaatatc attgacttcc ccaacgccct
    10801 gtctgtcgaa cgctttgctg gtggacgcgt tagcctcatg gaatttgtcg tcaagtccac
    10861 cagcggtctt tgccaaatgc ccatttctga ttttcgtaaa aaatttggta atgtcattgt
    10921 ctgtgcgata gagagggatc atcaaattat cattccaagc ggtgacatga ctgtacagga
    10981 taaagataga atctttgtca ctggtaaccg tgtcgatatg atactcttcc ataattattt
    11041 taaatcacgc gccgtgaaga gccttctcat cgttggggca ggtagaattg cctattatct
    11101 acttggtatt ctcaaagata gtcgtatcga tacaaaagtc attgaaatca atcctgaaat
    11161 cgccagcttc tttagcgaga aattcccaaa tctctacatc gttcaaggag atggaaccgc
    11221 aaaagatatc ctgctggaag aaagtgctca acactatgat gccgttgcga ctctaacagg
    11281 agtcgatgag gaaaatctga ttacatctat gttccttgac agggtaggtg tacagaaaaa
    11341 tattactaag gtcaatcgta ccagtctcct cgagattatc aatgcgcctg atttttcaag
    11401 tatcatcaca cctaaaagca tcgctgtaga tacgattatg cactttattc gtggtcgagt
    11461 taatgcccag tattcagacc ttcaagctat gcaccatcta gccaatggcc aaatcgaaac
    11521 cctgcaattc catatcaagg aagccaataa aatgactgcc aaacctcttt ctcaactgaa
    11581 attgaaaaaa ggggttctta ttgcagccat tattcgaaag ggcaagacta ttttcccaac
    11641 tggggaggat atgttggaag ttggagacaa gctcctagta acaaccttgt tgccaaacat
    11701 caccaagatt tatgacttga tcgcgaggta agaaatgaat aaaagtatga ttcgttacct
    11761 cctttcaaaa ttacttttga ttgaagctgt tcttcttttg gttcctgttg cgattgctgt
    11821 ctattaccgt gaatcgagcc aagtctttac agccctcttt tcgaccatag ggattctcgt
    11881 attgttaggc ggttcaggga gtttacagaa gccaaaaaat caacggattt atgccaagga
    11941 gggagtcttg atcgttgccc tctgttggat cctttggtct ttctttggtg gtctcccctt
    12001 tgtcttttct gagcaaatcc ccagccttat tgatgccttt tttgaaatta gttctgggtt
    12061 tacaactact ggagcaagta ttttgaacga cgtttcggtt ctcagccgtt ccctcctctt
    12121 ctggcgaagt tttacccact tgattggagg gatgggagtg cttgtttttg cacttgctat
    12181 tatggacaat gccaaaaata gccacctaga ggtgatgaag gctgaggttc caggtcctgt
    12241 ttttggcaag gttgtatcca aactaaaaaa cactgcccag attctctatc tcgtttatct
    12301 agctctcttc tccctctttg tcatcatcta ttatctagct ggtatgcctc tctacgatag
    12361 ttttgtcatt gctatgggga cagcgggaac tggaggcttt accgtctata acgacggaat
    12421 tgcccactat ggcagctcac tgattaccta tctggtcagt atcggagttc tggtttttgg
    12481 ggtaaatttc aatctctact actacctcat gctccgtcgc atcaaggcct tctttggtga
    12541 cgaagaactt cgggcttact tggtcattgt actggtttct acaggcttga ttagcctcaa
    12601 caccctctac ctctacccag ggttttcaaa gagctttgaa atgaccttct tccaggtttc
    12661 caacatcatt acaacaactg gttttggata tggagatatt accaactggc ccctcttctc
    12721 ccagtttatc ctccttttcc tcatggcaat cggtggctct gctggatcaa ctgcaggtgg
    12781 actcaagatt attcgaggcc tcatcctttc aaaaattgcc aaaaaccaaa ttttgtcaat
    12841 cctatcgccc caccgtgttt tgaccctcca tgtgaatcaa acggtgattg ataaagatac
    12901 ccagcataag attctcaagt actttgtcat ctatgctatg attttgctag cgcttatctt
    12961 tattgtcagc ctagatagca atgattttct ggtcgtgacc agcgctgtct ttagctgttt
    13021 caataatatc gggcctattc taggaaccac ttctagtttc tcaatcttta gtcccatctc
    13081 aaaaattctc ctatcctttg caatgattgc agggcgcttg gagatttacc caatcctcct
    13141 tctctttatg aagagaactt ggtctaagag ataaaataca aaatccctct ttttacaatt
    13201 gtgaaagagg gattcttata ttaaaaaagg gggaacgttt ttaaatccaa gcctatctgt
    13261 aagcaaaatc cttactcaaa taaaagatgg aaaaacttac tacacagctt ggtaatgctc
    13321 caatacaaat tgtaaggatt tacttggact ctttgttaaa tgaatatatt ccattcctct
    13381 ggtagcaact atctgaatac ctaatctttc tgcatccacc ttcatctctc tataataact
    13441 ccgaacttgt ggagccagaa taattaaatc ataaacaccc ataatatcat aatgagctcc
    13501 gtacgctcct gaattcgcaa tcactctaac ttctgctaat tgagcgccct cgttaattgc
    13561 attggctaat tgcgcacttg ttccagaccc tgcacaaaga accagtactt tcaactcctt
    13621 actttttatt tcatctaact caccaggcat tatttcactt gtctcctcct ctaaaataat
    13681 atcatttgag cttgcaacat cttctttcat cagtaactgt ctatcatacg ctctacagaa
    13741 tggcaaataa atcaatatgt cgacaactaa aatcaaagat aaaaatacaa aggagataga
    13801 ttgaaaattc gttccaatta acaatcccaa gggaccagga aaggcccaag gtaactggat
    13861 ataaaatcca ttcattccaa agaaatcaat aaagaccttt cctagaaata cattcactgg
    13921 tggagtcatc aaaaaaggta caaaaagata gggattcaaa ataactggca taccaaatag
    13981 aagagattca ttcaccgcaa ataaaacagg agtaattgta gctttaccta ccgattttaa
    14041 ttgtttagac cgcataaaga aaatcaaaat aaatggtaca acaaaggtag cccccgttcc
    14101 tccgatagca gcgatataat ttccgaaatt aggcgttaaa gaatgataag gaaactgccc
    14161 atttgcaaga agttgagcat tctcttccat attgctcagt tgtaaagctg taacagcagg
    14221 taagacaata gatggtccat gaattccaac aaaccaaagc aaagcgcaca taaaccaaat
    14281 caacatcata gcaggatatg attctgcccc cttaaaaata ggagtcaata gttgttgaaa
    14341 tacttgggca aaaggaacat ctaaactaaa ccgagataca atatctaaca aaccactaat
    14401 aagtaaaaca aaagaaaaag gaaaaatatc tctaaaagct tgtgatatag ccccaggaac
    14461 ttccttaggt aaatgaatag taatgtctcg cttaatacag aatcggtaaa catttacagt
    14521 aataaaggta ctgacaaacg aagttatcaa tccttgagat cctaaatagt ttgtcgacaa
    14581 acttgtagat cccgttttct catcaactac aagcgttaca gttaatacaa gatagccaca
    14641 tatggccgcc aacattgcag aagtatcatt tattaccttt ccaggaggca tttttctatt
    14701 aacatttcca gccaatgact ttgcaactgt ccctgaaaca ataacaccca aaactcccat
    14761 ggttagcata tacactttcc tcatccaggc aatagctgga ctcggtaact ctactccgaa
    14821 atttttagga atcataataa taatcatcaa aaagcttgaa aacataatta aaggcatagt
    14881 agcaataaat ccatctttaa tagccatcat ataattatta gtagaaattt tttcaaattt
    14941 cccttgttgc ttttcaatgg cagcgactaa cttatccata aaaactcctc ctgtaaaaaa
    15001 tttaagctca tagttaaaat atattaaaca tcagtcaaat cgctaacctt aattccgttt
    15061 taatcctttt acccaaagag cacttttctt aatatatctc ttctgtgttt cataatcaac
    15121 aaagaaaaga ccatatcgct tattgtagcc attcgcccaa gaaaattgat cttgcaaaga
    15181 ccatataaaa tagccttgaa tattgactcc cctatctcga gcctctaaaa ctttgtgtaa
    15241 atgttgctca atataacgga ttcttttact gtcatcaata atatcattct cgccctctgg
    15301 cttaacctct ttaagggctg taccattttc agttaaataa atgaccggat gttgaggata
    15361 ttcttctttg atacgaagca acatatcaaa taacccttga ggataaatat tccaatccca
    15421 atctgttgtc ggaataccag gcttttttac aaattcacct agagcattta atttaaaaga
    15481 agactgtcct tttattcctg ttgaattaaa ttcttgtcta ttttcacctt tgtattcacg
    15541 aataaattct gaacgataat aattcatccc aaacatcgta ttaagaggtg cagctctttt
    15601 cataatttct aaatcaccat cttcaataat aaagctagaa ttattagctt ccaatatttg
    15661 attcaagtga aaaagcgtgt catcactgta gtagcccaaa aaagttccat ctaatagaaa
    15721 tttattatta taaacatcat accgtttagc tgctaaaata ttttcttttt gcccatcaat
    15781 aggatagcct ggctttaaag catgaataca acctatcttt ccctcaatcc ctaattgatg
    15841 aaattcgagg actgcaagag catgcgccaa caacatatta tgattcgctt gaattgcttc
    15901 agataattga aaatgatgat ttggaggaaa ctgacctcct atatattgac ctgcagcaag
    15961 agacatcagt tcattgattg taaaccaatg cttgacttct gtaaattctt ggaaacaaaa
    16021 gcgagcatat cgtatgaaac gatcaatatt ctctctattc aaccaatccc ctgtttctaa
    16081 cattttctga ggcgaatcaa aatgatgtaa agaaacaaac ggaatcacat tatgttttaa
    16141 gcaagactga aagactctat ggtaataacg aacagctaat atattaggct ccccatctat
    16201 atcaggaaat atacgaaccc aagaaataga taaacgcaaa gcctgcaaac catgttctgc
    16261 cgccaaagct atatcctctt cgtaacgata ataaaaatca ctagctggat ctggtaagaa
    16321 cggactgttt tcttgcaaat aaacatccca catatttgtt cctttgccat ctaccctagt
    16381 tgcaccctct acttgataag cagcggtagc agctcctaat aaaaactcct tcggtaaggc
    16441 tttaacctgt aaattttcca tgactaatcc tcattgcaaa ccaatatgtt cttcaatctt
    16501 tcgaattcgt tcatattcat caataaaaaa cttgacctga tcatacaata gcatcgttgt
    16561 catcaaagta tcttgaccat gaatcatgat aaaactcact tcaaaattat ctccagatgc
    16621 ctcctccgct aataaattag tctgttctcg atgggcagac actattgagt cgtttgcaga
    16681 ctcaaccaat tctcttgcct gttcaaaata tccatctctc gcaaacgcca aagcatccat
    16741 caaatctgac cttgcatctc ctgcgtaagc gacaattgta aaaccaagca tatttatttc
    16801 ttcttttgat ttcatctttc ttcctcctca atcttgatcc atcttttaca catcattatt
    16861 aaaatgactt cgctacgata taaatagaaa gtcgacccta aatatcttaa aaatccttaa
    16921 atttcataat atttaggatc aatcttctct gaataccttg gttcattttc tatacttatg
    16981 atttataagt ggaaaccagc gtaatttttt gctacttcaa ttacacgata aacatccaaa
    17041 ttagattgga atagcagttc gtgcccacgg atataattca acacatttct ccattcatct
    17101 tgagcttgcc ttgtttttcc ggttgcttga agatataaag ccttcgccat cttaacaaca
    17161 tattcacgat gataactgag ctgtttccat tcatcctttt gactatttaa taacttagaa
    17221 atattttcct caataattgg caaaaaatta tcagctagat tgtaagctat ataataatga
    17281 ttcgctaaaa catcattttg acgactgcca attgcattaa aataatcaca tgaggaataa
    17341 ctggataatt tttctaaata ttcaacaaca gactgccaat tttccccata caaagcagaa
    17401 aagtattctt caatcaattc ttcataactt cttgtcttct tccagagcat ttcccccata
    17461 acataattag gaaaattatg agggaatccg gcacgtaatt cttgacacga aatgtaaccg
    17521 ttcaaatgta ggttagaaag ataagataca tctctgtaaa tggtttgact aattttcata
    17581 tagcctaaat cgccataatg agcacgccct aaaggatagt catatacgaa actatctcct
    17641 ttaaatgttt tttgccactc aaaaagataa gataaatttt cctcaagaga attcggaagt
    17701 ataattttat tacgcagata aggtttaggc gtaggtatgg aattgtcaaa atctacatct
    17761 gcataactca tttcaaatgt tcttgtaatc ggtgcaaaca tcatggtaaa gcgttcagga
    17821 ttatctaatt tttctttctg aggcgcccat agcaactcat ggtaaagcag aaaacaaatc
    17881 tttgtatcta atccctcact cgttaaagcc ctatccaatt gattgagaat acgaatatac
    17941 tgatccgaaa ccaattcttg tctacagttt tcgcattcac aaatattatt acgagcatcc
    18001 gacaaccata catgtaagta gttaacatca ggtcttttct tggcataatc cttgataatt
    18061 tctaccatct tatccgccac atctggattt gaaaaatcca gactggttaa aatcggagcc
    18121 gtattaaata attctcgttt cccgtttatt tcagcgacat acggtttctt ctcctctgaa
    18181 atactaagac ctgattccca gccaaatttt gaagagtaac ctaaaacctc acctgtccat
    18241 ccatgaccaa cacgatgatg aataagacct cttttttgca attctttatc caacctatca
    18301 ctcaattctt gtactaattc atttgagaat ttttctttat ttaggtatgg attaaactca
    18361 tgttcatacc aacgtttcaa aaaagagtaa ggattttcaa actggatgaa aaaactgttc
    18421 atcccaatct taggtagcca atcaatgaaa tctagtatat tttcaaatga atccgctccc
    18481 tctatacata ctcccctatg atagtaactg gctgtttcat ctatagatag ctctttatct
    18541 aaaaaatctt caaatcgtaa ctctggaaca aagtcatgtc tgcgccctgg tctagtgtac
    18601 acaaccccaa attcatgaaa cattcggtaa attccaataa gtaaacctac aattgtatta
    18661 gactcaattc tccccttacc cttgtctaat gagataagaa catggtcttt tctgtcctct
    18721 ttattgacta atcctacaaa aatatccgct tccttctcct cgacaagctc tatgttagtc
    18781 ttaaagaccc gattcatata gtaacttaat tcctctaagg caaagctaga ataagtattt
    18841 ttctgttgat caaatactct gataaccatc ttatttccct ccaacagtag ctttttcttt
    18901 ttcaagattg cgtttatctg caattttgaa gaatggataa taaattgcta cagaaaccaa
    18961 aatcaaaaca acttgtaata gagctcctcg ccaactagcc cctgttgcaa ggaagcctcc
    19021 tataatcggt ggcattgtcc acggaaggat tacacctgtt gtatagggta ctaatcctac
    19081 agccattgat acataggtaa tcaaggcatt gattgtagga gtagcaataa acggaatcag
    19141 cataatcgga tttaaaactg ttggaaacgt aaatagaata gctgtattga tattaaaaat
    19201 agacggtata atagctagct tacctaatgt tttattcgca cgactcttac taaataggaa
    19261 gagacaaatc gcaagaccaa tagtcgctcc accgccacca ataaatacaa ataaatcttt
    19321 aaacggtaat gtaataatgt gttggagtgt ttgtcctgca gccacagctt cttggttttc
    19381 tgtagtaaat tgtaaccaaa ctgggtctac aaaagcattt aaaacttgtc ccccattaac
    19441 tccacagaac caaaagaatg agtttacaat aacacataga atcatacctg gaagcgttcc
    19501 tgcaattaac ttaagcggtg ttccaacaat tgcacctagg agtccgttga gacctcctgc
    19561 aactcctgcc gcttcaagac cttttaagac caaagcccac aaaacaaaag tagtaaaacc
    19621 aggtaaaaga gctgaaaatg atttacttac tacatctggg acaccacttg gcatttttat
    19681 cgtaattccg cgctggataa acatacgata aatctctgct gtaaccaatc caatagtaat
    19741 cgccataaac aaagaagatg cattcaggct agaaaatggt attgcgccgc caaataactg
    19801 cttgacctgc tccccatttt tatcataaac catactcgaa aaacgaggtg ccatcaatac
    19861 aaaactggat aaggctatga tccctgccga cggaccatct gtaccatatc cttccgacaa
    19921 cctgtaggca ataccgaaac aagcgaccaa ggagataatc gctactgttg actgattcat
    19981 agttgtcaag atacttagca atccaacact attcagccaa tttgtaaaag cctcttgagg
    20041 ccaactgatt agaataagaa ataaagagcc gactaaaatc aaaggcattg ccatcataaa
    20101 tccttcacga atagctttca aatgccgttg gttgccaact ttttcaccaa acggagcgac
    20161 ttttgtttct aaaaacgcta aaatattgtt catcaccgga actccttttt ttatttttta
    20221 taaattaaat ataccactaa cagaaagcgt tgtcaatata ttttttgttg tttttacaat
    20281 atttttttct ttgtatagaa taataatttt aattgatttt ttcttatgtt taatataaaa
    20341 taaaccaaag gaggaaacaa atgattacaa aacaatcagc ctttttacaa aatagagcca
    20401 ctatactgga gtttctatat cgaaatccag caacatcccg tacagatatt gttaatgaaa
    20461 caggacttac tccagcaaca acgactaaca tcataaaaga gctaagcgaa cagtccctca
    20521 tatacgaaac tggtgatgaa tttagcgagt tttccggatc tggaagacgt agaaaaacta
    20581 tttctatcac agataatatt ccgtatgttg ttggaggtat tgaaataaac gtcctaggta
    20641 tctttctcag tctatgtgac ttacaaggga aaactctttt cgagacagaa attttgaatg
    20701 aagattatcc tatttcagaa atcaattcca ccattaccaa tatgataaaa acagctatag
    20761 agtacgtccc tttggaaaca aaattacttg gatttggctt atcaatacct ggacattata
    20821 acaaagactc cggaagtatc attacaaaca accccatatg ggaatctttt aatttattaa
    20881 atgtaattaa aagattcaat tttcctttta ttgtaaaaaa taatatcgat tgtatggcta
    20941 taggacaata cctttttaat ccacacaata cccccgataa ctttattttc ctacacgctg
    21001 gattaggtat ttacacttcc tttttcacaa aagaaaaaat aggagcctct aaaaatcctt
    21061 atatcggaga aattggacac accattgtcg aattgaatgg gcaatattgt gaatgcggaa
    21121 aaaaaggttg tttacaaaca tatatttcgg atgcttggtt aatcaaacac gcccaattat
    21181 tatttaaaaa ttcccaacta actgtactaa aaagccttgt aaagactgaa aaagacattc
    21241 atttagacac ccttttaacg gcttataatt taggcgactc cgctttacgt caacaaattg
    21301 ataaaggagt caatttatta gccacttcta ttgcaaatct cctcctcatc aatcctgctg
    21361 ataaaatcta tatcaacagt caattgctta attatcaacc tttcactcat gaagtcaggg
    21421 ataaaatcca agaccagctc cacttcgttc cctttactcg taatatagaa attgaaattt
    21481 taccttacaa caaacatcgt ggaagtatag gagcttgtgc attagctatc gtcgcttttt
    21541 tcatagaaca tagcaatgta ttacaagata ttatttcacc ttaatatatt agaaatctat
    21601 agacctgttt aaatcaacta taacctgtag tagatatctc gtatttagac aatatgaaaa
    21661 caagacgact tccatatagg aaaccgcctt ctcgctatgt tgagtgattt atattaaaat
    21721 aacttttctt ctagctgcat tttattatta taaaaacatt catcataacc cccagaactt
    21781 aaataacaat ttttattcaa gatacatact cctagaataa actttatatg aaattctcat
    21841 ttttgttttt acaattctcc ttagttaaat cttgtttaat atatgtttta catatagtat
    21901 ttagcgccac atagtactga actctctcca aaaacggtta ttcctctttg aatagggcgt
    21961 tatcacaaga aaagcatctc cacgtttcaa cttcatatgg ctcaaaaaca atcaattgat
    22021 gctaaaacct gtacctagat gtttcggttc ataaaaccat gaaactgtaa aagtggatga
    22081 aattgatagc gatagtcaaa tcaagaggca tcataactct aaaaagtcac aatatataag
    22141 ttcatcctcg gaaaaatatc attctaattg ttgaaatgcc tacatgaaaa gaaacgtcaa
    22201 atgctcatga aacaacgaat acaggtatca aaactatgac aaaacaagtc cctaaattta
    22261 ctaaagacac tgctcaactt tacacctgta aatggttgtt gtataataaa gttacaaaga
    22321 tgtacgacca cactgttgta aatcatagtg ttcgcgaata tattactgat agcatttcta
    22381 caaatacaat tgaagagact ggatagactt taaacgaaat atgtcagtgt tttggcattc
    22441 ctagccttca tatcatttaa agaattctat agacaaaatt tttcccaata cagacactcc
    22501 taacaactgc ttcatttttc taccaacata tttaggaaca ggataagata caagaggatt
    22561 aatccataga tcagttctat accaatctaa gacaaataag ctaaaaaaac gattgataat
    22621 aagcaaatag attccaaatt ttctctatct gctcatttta ataaacaata ctagtgtaac
    22681 tatccttcca gtcagaagct tgtcaaatca caccgaaaat tcttctaaaa tttatctcgt
    22741 taggcaatca agcaaaaact cgacgatagt acaaacatta tcatacagaa ttgacttcct
    22801 aaattatata ctttagtaag gttttcgtat aagaaaaaag attcatttta catttctaaa
    22861 cattcttttc taagatgaaa aacagaattt ttcgattgtg atttaaagca acaagaagat
    22921 tttcagtatc atcctataga tacgagctaa ttaagaaaaa ctacattttc gaatataaac
    22981 tacaataata taaactaaat tttataggag gaagacaatg gattggtacg attatatgat
    23041 acaggcatcc aaacaatcac aattcaacgc aagccattgg tttcgctatt tgcgaaaagt
    23101 tatttttgaa gactattctt atttaacaaa ccaagatgta gaaaagttgc tagactccaa
    23161 agaactaacc cgttttcaaa aaattagctt gaagtatgcc tttcaagagc atactccaac
    23221 tcataaatat gtgatttcat taaataaacc tgctaagtta accaatgttc aaaaattgat
    23281 ggagaaatac aaacatggat aaaatgaaac cggtcttcca agccctaaat aaggaattaa
    23341 ttcaggaaaa tctgacttta acaattatct gtgtcgatgg ttatgtctta gaatatcatg
    23401 gtttacgtgc cacacaagat gttgatgctt tttatgacca aaatcagaaa atcaatgaaa
    23461 ttattgctcg tgtaggaaaa cagtttaatc ttaacatcca cgaagagtta tggctcaaca
    23521 atcatgttgc aaagcaaata tgaacaaaca acctccctta agtttatgcg aatccttgta
    23581 ctcatttgag aatctgaccg ttttagtagt ccctattgag tatgtgttag gaatgaagat
    23641 gatgagtatc agagagcagg atttgcaaga tattggtgca attattaaat acaaaaactt
    23701 tcattcacca ttcgatacct ttaaatattt aaaagacatg ggatttgata ctattgacct
    23761 ctctgttcta ttagaaggtt ttagttatgc ttatggtatg gattggttag aaaaattttt
    23821 taaagaaaat caagataaac ttcgcgaatt ttattaacag tatcaaacaa gatgattgtc
    23881 aattagccag tcgtcttgtt ttttatgatg aaatgaaata aaatctgaac aaatcgctta
    23941 gagaattcca gtcaatttct agcgctgttt gagacgtttc agtggactat agtaaaatga
    24001 aataagaata ggacaaatcg atcaggacag tcaaatcgat ttctaagaat gttttagaaa
    24061 taaaggtgta ctattctagt ttcaatctat tatatttcag attcggtcca ctattccttc
    24121 ccaaaaacat tgcatattgc gtcaaaggaa ttgctcacac tatcccaagc acaaaaccct
    24181 cagtcgattg accgagggtt cctttttatt attcaagaac ttgattagct caatgcatcc
    24241 aaggcgtcat ccattgaaag aacttcgtgg aagacacgtt gtgtcaattc agttttttgt
    24301 tctggagtga ggtatttagt gtttacacag tatccagaga tacgtacgat aacgtcttcg
    24361 cctgacatga tcttttcgta aacatcgttc aagtccataa cgttcaagtt aacgtgttgt
    24421 ccaccgtttt cgaagtaacc atcaaggatt gttaccaagt tatcaacttg ttcatcacga
    24481 gtcttaccaa gagcgcgagg tgatacttgt gtagtcaatg agataccgtc agctgcataa
    24541 ctaaagtcaa ggctagaaag tgagttcaag ttttgcaacc aaccaccttt agctttgtta
    24601 gatgggttag cacctggtga gaagaattca agtttagaca agttcacaga accatcttcg
    24661 ttgaggtata cacctttgtg aactggtgag ttaccagttt gtttagagta agcaacgtta
    24721 gatgtgattg tcaaaagtga tactgtagct tctgcgtctt tgtatagttt gtggctacgt
    24781 agacgagttg tgtaagcttc gatcaaccat tctgccaatt cgtttgaacg tgggtcatct
    24841 tcaccccagc gtgggtagtc accgattgtt tcgtaatcgt agatgtagcc atcttcgtca
    24901 cggattggtt taactgtagc gtatttgata gctgacaatg tatcaacagt gttagcaaat
    24961 ccacagatac cgaatcccat gttggcacgt tgtttagttg gcaagaaggc catttgaaca
    25021 gcttcgtagt tgtacctatc agtcatgtag tggatgatgt tcaaggcatc tacgtaagtg
    25081 tcagtcaacc agtcaagaga tttttcaaag ttcgctttaa ctgattcgaa ttcaagaact
    25141 tcgtcacgga ttggttcgat atcaaatact ttgtagtctt tgtgaacatc gtcgtaacca
    25201 ccattcaaac cagtaagaag ggctttaaga acgtttacac gagcaccgaa gtactggatg
    25261 ttgtggcgtt gttcttcatt ttctggatca agtggagaca cacagcatga gatacagctc
    25321 atttcaccat atccgtcttt agccattgtt gttacacctt cgtattggat agaagagtgt
    25381 ttgtggctca tgtgcataca gtagcgacgg aagttgtatg gcaatttgtc agtccaaaga
    25441 actgtcaagt ttggttctgg tgagttaccg atgttgtcaa gagtgttcaa gaaacggtag
    25501 tccatcttag taacacggtg acgaccgtcg ttacccatac cagccataga agttgtgata
    25561 aaggttgggt cacctgagta caattggtca taagcttttg tacgagcaaa tttaactgta
    25621 cgaagtttca taacgaaatc atcaacgaat tcttggattt ctgattcagt aaatgtacca
    25681 cgagcaaggt cacgttctgc aaagatgtcc aatacgattg gtacacgacc tagagatgta
    25741 gcagcaccgt taatcacacg gcagacagcc atgaaagcaa tgttaaccca ttggattgct
    25801 tctttcacgt tcatcgctgg tttgcgaaca tcaaccccgt aaaggtcacc caggcgaaca
    25861 acttgttgca atgcttggta ttgaaggttt acttcttcac gaagacggat tgtttcttca
    25921 tcgatttctt tgattgcatt ccagtcgttt actttttctt gcatcaagta gtctgcaccg
    25981 taaagagcaa gacgtgcgta aacaccgatg atacgtccgc gtgagtatgc atctggaaga
    26041 ccagttacag tgtgtgcatg acgagcgcga cgaatatttg aagtgtaggc acggaaaata
    26101 ccgtcgttaa ctgttgttac atatttagtg aagatttcgt gaacagctgg gtctggttcg
    26161 tatccatttt cttttaaagt agtttcagcc atacggatac caccttttgg catgaagttc
    26221 aatttgaaga gttcatcatt ttggatacca aagataactt cattttcttt gtcgataaat
    26281 ccagcaggga tatcagcgat agatgttgga cgagtgtcca ttgggaaacg agtttcttcg
    26341 tagtgtgctt tagtttcttc tacaattttc ttgatgtgaa gtgaacgctc tgttggtcct
    26401 gcaaggaagc tttcgtctcc atcataaggt gtgtagttag cttgtacaaa gcgtgataca
    26461 cttgcttttt ctttccaatc tacgcctttg aagccttccc aagctttgtc aaaaatatct
    26521 tgtgcttcaa caactgtctt aacaaccata ttaatatcct cttttttcta gtaacagtca
    26581 tctgttacat tcataagaca agtataccat aaagtaaccg atttcaacaa gtgcaaaatc
    26641 cctattttta cactttcttt tctaaaacag tctatatttt atttcaaact gtattatatt
    26701 tttgaaaaaa ataaagtcct tttttctttt tttcagaaaa aagggtataa taaaagaaaa
    26761 taagcagtaa cactcaatgg aaatcgaaaa gcaaactagg aagctagccg cagattgctc
    26821 aaaacactgt tttgaggttg cagatagagc tgacgtggtt tgaagagatt tttgaaaagt
    26881 ataaaaaggt gctaggcatg ttgatttttc ctttgttaaa tgatttgtca agaaaaatca
    26941 tccatattga catggatgcc ttttttgctg cagtggaaat cagggataat cctaaactca
    27001 gaggaaaacc tgtcattatt ggaagcaacc ctcggcaaac aggtggacgg ggagtcgttt
    27061 ctacctgtag ttatgaggca agagcttttg gtgtccattc tgccatgagt tccaaggaag
    27121 cttatgaacg ttgtccccag gctgtcttta tctcagggaa ttatgagaaa tacaagtctg
    27181 tgggactcca gattcgagct atttttaagc gctatacaga tttgattgaa cccatgagta
    27241 ttgacgaagc ctatttggat gtgacagaaa ataaactcgg tatcaagtca gcggtcaaaa
    27301 ttgctcgcct cattcaaaaa gatatctggc aagaactcca tctaactgct tctgccggta
    27361 tttcctataa caagttctta gctaaaatgg cgagtgatta tcaaaaacct catggtttga
    27421 cagtgattct acctgaacag gctgaggatt ttctcaaaca aatggatatt tccaaatttc
    27481 atggagtagg aaaaaagaca gtagaacgtc ttcatcaaat gggcgttttt actggtgctg
    27541 atttacttga agttcctgag gtgaccctaa tagaccgttt tggtagacta ggctatgatc
    27601 tgtatcgaaa ggctcgtggc attcacaact ctccagtcaa atccaatcgc atccgtaaat
    27661 caatcggcaa ggagaaaacc tacgggaaga ttctccgtgc tgaggaagat atcaaaaaag
    27721 agctgactct tctatcagaa aaagtcgctc tcaatctaca tcaacaagaa aaagctggaa
    27781 aaattgtcat tttgaaaatc cgctacgagg acttttcaac tcttaccaaa cgaaaaagtc
    27841 ttgctcaaaa aacacaagat gctagtcaga taagccaaat agccctgcaa ctctatgaag
    27901 aattaagtga gaaagaaaga ggtgtccgcc tattggggat taccatgact ggattttaaa
    27961 gctcaatgaa aatcaaagag caaactagga ggctagccgc aggctgctca aaacactgtt
    28021 ttgaggttgc agataaagct gacgcggttt gaagagattt tctaagagta taaaaccttg
    28081 aagagttgcc ccttcaaggt ttttcttata caaataaacg gacaaaactg taaagtagca
    28141 aaacactacc aagcacgata cggtatttac caaaaagggt gaagtcgtgt tttttcacat
    28201 agctggtcaa gaagcgaata gccaccatgc tgaccgcaaa agctactccc atcgcgacca
    28261 agagcaaaaa caattgccca aagctcaaga gttctccggc tttcacaaat ttgaaaatct
    28321 ttaaggcact agctccaaac ataacaggaa tcccaagata gaaggtaaat tctgtcacaa
    28381 ctgaacgact ggttccattt aacaaaccac cgacaatcgt tgcacctgaa cggctagtcc
    28441 ctggtaaaag agcaagaact tggaagagtc cgatatagaa agcggtcgta taaggaagct
    28501 tgtccaactc tgttacactt ggctcgatag cacgcgcttt attgcgcttt tccaaataga
    28561 tgaaggcaat cccgtagata atcaacatga gagcaactga aaccatgtta tggaagtggg
    28621 tatcaaacca atcatcaaat ttaaagacac caagtaaagg taaagtagca atcaagacct
    28681 tcaaccatag tctccaagtc ttacgaactt cctgtttgtc cttagtcggt ttaaaaggat
    28741 tgagcttgtt aaaataaatc accataactg ctaaaatagc accaagctga atcacgacat
    28801 taaacatgga cataaaggct tcattttgat tttggtattg gataaactcc tctgctaaaa
    28861 tcaagtgacc tgtactggaa atcggcaacc attccgtaat tccttcaaca atcccgaaga
    28921 agatagattt taaaatttca ataagataca tatatttctc cttatattat attta
//
LOCUS       DAVKHZ010000017        27886 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000017 DAVKHZ010000000
VERSION     DAVKHZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.017"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            72..1241
                     /gene="pspC"
                     /locus_tag="VWW99_000715"
     CDS             72..1241
                     /gene="pspC"
                     /locus_tag="VWW99_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HEU9538591.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPSPDTKPSPQPEGKKPSVPDINQEKEKAKLAVVTYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQPEKPKPDNSKPQADDKKPSTTNNL
                     SKDKQPSNQASTNEKATNKPKKSLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(1460..2440)
                     /gene="dusB"
                     /locus_tag="VWW99_000716"
     CDS             complement(1460..2440)
                     /gene="dusB"
                     /locus_tag="VWW99_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9538592.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(2427..3299)
                     /gene="hslO"
                     /locus_tag="VWW99_000717"
     CDS             complement(2427..3299)
                     /gene="hslO"
                     /locus_tag="VWW99_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9538593.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKIAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            3464..4891
                     /locus_tag="VWW99_000718"
     CDS             3464..4891
                     /locus_tag="VWW99_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9538594.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLFY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            5050..6558
                     /gene="glpK"
                     /locus_tag="VWW99_000719"
     CDS             5050..6558
                     /gene="glpK"
                     /locus_tag="VWW99_000719"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9538595.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            6600..8426
                     /gene="glpO"
                     /locus_tag="VWW99_000720"
     CDS             6600..8426
                     /gene="glpO"
                     /locus_tag="VWW99_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9538596.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            8496..9200
                     /locus_tag="VWW99_000721"
     CDS             8496..9200
                     /locus_tag="VWW99_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9538597.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            9497..9700
                     /locus_tag="VWW99_000722"
     CDS             9497..9700
                     /locus_tag="VWW99_000722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538598.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(9787..10395)
                     /locus_tag="VWW99_000723"
     CDS             complement(9787..10395)
                     /locus_tag="VWW99_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538599.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            11527..11928
                     /locus_tag="VWW99_000724"
     CDS             11527..11928
                     /locus_tag="VWW99_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase"
                     /protein_id="HEU9538600.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPILKVEIPKPDGGIRQLGISTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYE"
     gene            11932..12243
                     /locus_tag="VWW99_000725"
     CDS             11932..12243
                     /locus_tag="VWW99_000725"
                     /inference="COORDINATES: protein motif:HMM:NF012307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538601.1"
                     /translation="MDIDLEKFFDTVPQDRLMSLVHNIIEDGDTESLIRKYLHSGVII
                     NGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLRFVRYADDCVIRSEARQPLS
                     V"
     gene            12201..12803
                     /locus_tag="VWW99_000726"
     CDS             12201..12803
                     /locus_tag="VWW99_000726"
                     /inference="COORDINATES: protein motif:HMM:NF019984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538602.1"
                     /translation="MCDTVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELK
                     YLGFGFWKSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINY
                     FSLGNMKSIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDH
                     YQLVAQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            13260..14438
                     /locus_tag="VWW99_000727"
     CDS             13260..14438
                     /locus_tag="VWW99_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9538603.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFRAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            14752..14883
                     /locus_tag="VWW99_000728"
     CDS             14752..14883
                     /locus_tag="VWW99_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9538604.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            14904..16454
                     /gene="dltA"
                     /locus_tag="VWW99_000729"
     CDS             14904..16454
                     /gene="dltA"
                     /locus_tag="VWW99_000729"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9538605.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            16451..17695
                     /gene="dltB"
                     /locus_tag="VWW99_000730"
     CDS             16451..17695
                     /gene="dltB"
                     /locus_tag="VWW99_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9538606.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            17709..17948
                     /gene="dltC"
                     /locus_tag="VWW99_000731"
     CDS             17709..17948
                     /gene="dltC"
                     /locus_tag="VWW99_000731"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9538607.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            17941..19209
                     /gene="dltD"
                     /locus_tag="VWW99_000732"
     CDS             17941..19209
                     /gene="dltD"
                     /locus_tag="VWW99_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9538608.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAILAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            19364..19804
                     /gene="adcR"
                     /locus_tag="VWW99_000733"
     CDS             19364..19804
                     /gene="adcR"
                     /locus_tag="VWW99_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9538609.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            19804..20508
                     /locus_tag="VWW99_000734"
     CDS             19804..20508
                     /locus_tag="VWW99_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9538610.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            20501..21307
                     /locus_tag="VWW99_000735"
     CDS             20501..21307
                     /locus_tag="VWW99_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9538611.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            21317..22822
                     /gene="adcA"
                     /locus_tag="VWW99_000736"
     CDS             21317..22822
                     /gene="adcA"
                     /locus_tag="VWW99_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9538612.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQSLDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKVLTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(23993..24766)
                     /locus_tag="VWW99_000737"
     CDS             complement(23993..24766)
                     /locus_tag="VWW99_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9538613.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            24942..26345
                     /locus_tag="VWW99_000738"
     CDS             24942..26345
                     /locus_tag="VWW99_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9538614.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSTLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEDSLFISSGTWSLVGVELSSPILSTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETGEWTDGQLFKIVYESLAETYKETIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEATAVGNICIQLLSQGKINEDTGLK
                     DIMTNIADTQYYYPQLS"
     gene            26374..27666
                     /locus_tag="VWW99_000739"
     CDS             26374..27666
                     /locus_tag="VWW99_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9538615.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWEDFTQAMAAVRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
BASE COUNT         8478 a         5120 c         6027 g         8261 t
ORIGIN      
        1 tagcaaattt catatacaat tcatattagc aggatatact gtagttaatt aaaattatgg
       61 agaatataga catgtttaaa tcaaatcatg agcgaagaat gcgttattcc attcgtaaat
      121 ttagtgtagg agtagctagc gtagctgttg ccagtctttt tatgggaagt gttgtacatg
      181 cgacagagaa agagggaagt acccaagcag ccacttcttt taatagggga aatggaagtc
      241 aggcagaaca acgtggagaa ctcgatttag aacgagataa ggcaatgaaa gcggtcagtg
      301 aatatgtagg aaaaatggtg agagatgcct atgtaaaatc agatagaaaa cgacataaaa
      361 atactgtagc tctagttaac cagttgggaa acattaagaa caggtatttg aatgaaatag
      421 ttcattcaac ctcaaaaagc caactacagg aactgatgat gaagagtcaa tcagaagtag
      481 atgaagctgt gtctaaattt gaaaaggact cattttcttc gtcaagttca ggatcctcca
      541 ctaaaccaga aactccgcag ccggaaaatc cagagcatca aaaaccaaca actccatctc
      601 cggataccaa accaagccct caaccagaag gcaagaaacc aagcgtacca gacattaatc
      661 aggaaaaaga aaaagctaag cttgctgtag taacctacat gagcaagatt ttagatgata
      721 tacaaaaaca tcatctgcag aaagaaaaac atcgtcagat tgttgctctt attaaggagc
      781 ttgatgagct taaaaagcaa gctctttctg aaattgataa tgtaaatacc aaagtagaaa
      841 ttgaaaatac agtccacaag atatttgcag acatggatgc agttgtgact aaattcaaaa
      901 aaggcttaac tcaggacaca ccaaaagaac caggtaacaa aaaaccatct gctccaaaac
      961 caggtatgca accaagtcct caaccagagg ttaaaccgca gccggaaaaa ccaaaaccag
     1021 ataatagcaa gccacaagca gatgataaga agccatcaac tacaaataat ttaagcaagg
     1081 acaagcaacc ttctaaccaa gcttcaacaa acgaaaaagc aacaaataaa ccgaagaagt
     1141 cattgccatc aactggatct atttcaaatc tagcacttga aattgcaggt cttcttacct
     1201 tggcgggggc aaccattctt gctaagaaaa gaatgaaata gtgattgatt cattcaaaaa
     1261 tgtttgtaaa aagtattcgg cttaaaagaa aagagtctgg gataaaaaga ttttgatttt
     1321 aggaattctt tattataaat ttttaaaatc gatagattag acaaaaaagc gaacaagaca
     1381 gaattctgag tgtcagataa ctgattttgt tcgcttttta tatttaatat tatacttttg
     1441 tcctaggctc ttttttgcat tatacttttt ccaattgcaa gagggcttca atctctgcca
     1501 gggtgctagc ttgcgaaatg gctccacgga gtttggcagc gccagatgtt ccccggagat
     1561 agtgaggagc gaggccgcgg aattcacgaa ctgcgacgtt ttctcctttg aggttaatca
     1621 atcgtttcaa gtgttcgtag gcgatcttca tcttgtcttc aaaggtcaaa tcaggtagga
     1681 tttctcctgt ttcaaagtaa tggttgattt ggttgaagag gtaaggattt cccatggcag
     1741 ctcggccaat catgactgcg tcagcaccaa cttcttcgat gcgttgcttg gcttcttgga
     1801 cagtacggat atcaccgttg gcgatgaatg gaatcttggt tagagcttgg gcaaccttgt
     1861 aaagggtctc aaggtctgcg tggccagtat acatttgttc acgggtacgg ccatgcatgg
     1921 cgagggcaga aacacctgca gcctcagcag cgagggcatt ttctactgca agagatgggt
     1981 ccgcccagcc ggtacgcatt ttgacagtaa gtgggatatc aaggacagac tggaccttgt
     2041 tgatgataga gtagatcttg tcaggatcct tgagccacat agctccagct tcgttcttca
     2101 cgattttgtt gacagggcag cccatgttga tatcgacgat atcggtcttg gtgttttctt
     2161 ggatgaattc tgctgcgcgt gctaggctgt cttcatcgct accaaaaagt tggatagaga
     2221 cagggttttc gccctcatcg atatgaagca tgtgcagggt tttttcgttg ttgtattgga
     2281 ttcccttgtc agagaccatt tccattacaa cgagtccagc tccgagctct tttgcgatgg
     2341 tacgaaaggc tgagttggtc acgccagcca taggcgctaa aacggtacga ttgggaatct
     2401 caacattgcc aatcataaaa ggtgtattaa gatttgtcac gaatgagttc ctccaggtcc
     2461 ttttcatcaa agttgtaagt agtttggcag aattgacaag tgatttctac cccgtggtct
     2521 tcctctttca tttcctgtaa gtctgagctt ggaaggctgg caagagcgtt cataaagcgt
     2581 tcatggctac agtcacattg gaaacggatt tcttcttcag aaagacgctt gtaggcttcg
     2641 tccccgtaga tagccttgag gagggcttcg atatggtcgt cgctttcgag aagagtagag
     2701 atagctggca tttcttggat gcgtttttca aagcgagcaa tctcttcttt cttggctcct
     2761 ggcaagactt gaactaggaa accacctgca atcttgacct tgtcttcctc gtccaaaagg
     2821 acattgaggc cgaccgctga aggcgtttgt tggctttcag taaggtaaaa ggcaaggtct
     2881 tcaccgattt ctccagagat gaggggagtt atagagttgt aaggatttcc agtaccgtag
     2941 tctgtgataa cgaggaattg accatttcca acaaaaggtc cgactaggac ttcaccagtc
     3001 gcagtctttt tgatgtcaac accaggattt tgaacgtagc ctttgacgtt ccccttggta
     3061 tcagcgacgg tgataatagc acctagagag ctagatccca acaccttaac tgtaagtttg
     3121 gtatttcctt tttcattggc tgcgagaatc tggctggcga taagagttcg accaagcgct
     3181 acagttgagc tagcttgggt ttgatgtttt tcttgagcag tgcggacggt ttcagtgcta
     3241 tcaaggacaa aagcacgaaa ggctccgctt tctgatatag ttttaataat tttatccata
     3301 gctactattt tagcataaaa atgcccaaag ggggagccgt gtgtttactg attttcagga
     3361 taatggacca ggaaatcagc atgaaaataa aaagagaaac agattatttc agcatttgta
     3421 agatttatgc tatgcttaag gtagaaaatg aaaggggtaa caaatgtatt taggagattt
     3481 gatggagaaa gccgagtgtg gtcaattttc aatcctttcc tttctattac aagagtctca
     3541 gacgaccgtc aaggctgtaa tggaagaaac aggattttca aaagcaaccc taaccaaata
     3601 tgtcaccctg ctcaatgaca aggctttgga tagtggctta gaactgacta ttcactcaga
     3661 agatgaaaat ctgcgtctgt ctatcggtgc agctaccaag gggagagata ttcggagctt
     3721 gtttttggag agtgctgtta aataccagat tttggtttat cttttctacc accaacagtt
     3781 tttagcccat cagctggctc aagaattggt gattagcgag gctacgcttg gtcgtcactt
     3841 ggctggttta aatcagattt tgtcagaatt tgatttatcc atccaaaatg gacgttggcg
     3901 aggtccagag catcagattc gctatttcta tttctgtctt ttccgaaagg tctggtcgag
     3961 tcaggaatgg gaaggtcaca tgcagaaacc agagagaaaa caggagattg ccaatttaga
     4021 ggaaatctgc ggtgcaagtt tgtctgtggg gcagaaattg gacttggttc tctgggctca
     4081 catcagtcaa caacgtcttc gggtcaatgc ttgtcagttt caagtcatag aagagaaaat
     4141 gcgagggtat tttgacaata tcttctatct tcgtttgctg agaaaggttc cgtccttttt
     4201 tgctgggcaa catattccac taggagttga ggatggtgag atgatgatat tcttctcttt
     4261 tctcctatct catcgcattc ttcctcttca tactatggag tatattcttg gttttggagg
     4321 gcagttggca gatttactga cgcaattgat tcaagaaatg aagaaggagg aactattggg
     4381 ggattataca gaggaccatg tcacctatga actcagtcag ctttgtgctc aagtctatct
     4441 ctataagggc tatattttac aggatcgcta caagtaccag ttagagaatc gtcatccata
     4501 tttactgatg gaacatgatt ttaaagagac agcagaggag atttttcatg ctctacctgc
     4561 ttttcaacag gggacagatt tagataagaa gattctctgg gaatggctcc agttaatcga
     4621 atatatggct gaaaacggtg gtcagcacat gcggattggt ctggatttga catctggttt
     4681 tcttgtcttt tcaaggatgg cagccatttt gaaacggtat ttggaataca atcgttttat
     4741 taccattgaa gcttatgacc ctagtcggca ttatgatttg ctggttacca ataacccgat
     4801 tcataagaag gaacagacac cagtctatta tttaaaaaat gacttggata tggaggattt
     4861 ggtagcgatt cgccagttat tattcactta aaaggcttgg ttaatccagg tcttttttgt
     4921 gaaattcaca caatctcctc acattttttt aaaaattaaa aaaagttgat aaacaagaaa
     4981 gcgctttatt ttgtatacta gtaagtgtaa agaggaaaca cctcaagatc tttatcagga
     5041 ggacagtaca tgtcacaaga aaaatacatc atggccattg accagggaac tacaagttct
     5101 cgtgccatca ttttcaacaa aaaaggggaa aaggttagct cgagtcaaaa agagtttacc
     5161 cagattttcc ctcaagcagg ttgggttgag cacaatgcca atgaaatttg gaactctgtt
     5221 cagtcagtta ttgcgggtgc tttcatcgaa agtggtgtca agccaaatca aatcgaggca
     5281 atcgggatta ccaaccaacg tgaaacaacg gttgtctggg ataagaaaac aggacttcct
     5341 atctacaatg ctatcgtttg gcagtcacgc cagacagcac ctttggctga gcaactaaaa
     5401 agccaaggtt atgtggaaaa attccatgaa aagactggtt tgattattga tgcttacttc
     5461 tctgctacca aggttcgttg gattttggat catgtagaag gtgctcaaga gcgagcagaa
     5521 aaaggggaat tgctctttgg tactatcgat acttggttgg tttggaaatt gactgacggt
     5581 gcggctcacg tgactgacta ctcaaatgca gctcgtacca tgctttataa cattaaagaa
     5641 ctcaaatggg atgatgagat tttggaaatc cttaacattc cgaaggctat acttccagaa
     5701 gttcgttcta actccgaaat ctacggcaag acagctccat tccatttcta cggtggagag
     5761 gtgccaatct caggtatggc tggggaccaa caagcagccc tctttggaca gttggctttt
     5821 gagccaggta tggttaagaa tacttatgga acaggctctt tcatcatcat gaatactggg
     5881 gaagagatgc agttgtctga aaacaacctc ttgacaacca ttggttacgg aatcaacggt
     5941 aaggtttatt atgccttgga aggttctatc ttcatcgcag gaagtgctat tcagtggctt
     6001 cgtgacggtc ttcgcatggt tgaaaattca ccagaatctg aaaaatacgc tcgtgattct
     6061 cacaacaacg atgaagttta tgtcgttcca gcctttacag gtctaggcgc tccatactgg
     6121 aaccaaaatg ctcgtggttc cgtctttggt ttgactcgtg gaacaagcaa agaagacttt
     6181 atcaaggcga ctttgcaatc tattgcttat caagtgcgtg atatcatcga caccatgcaa
     6241 gtggatactc agaccgccat tcaagtactg aaggtggatg gtggtgcagc catgaacaac
     6301 ttcctcatgc agttccaggc ggatatttta ggcattgaca ttgcacgtgc taaaaacctg
     6361 gaaacaacag ctctaggagc ggccttccta gcaggtttgt cagtagggta ctggaaagac
     6421 ttggacgagt tgaaactctt gaacgagaca ggagaactct ttgagccatc tatgaacgaa
     6481 tctcgcaagg aacaactcta caagggctgg aagaaggctg tgaaagcaac tcaagtcttt
     6541 gcggaagtag acgactaata ctggcagaat aaagcgattt atttagaaag tgtgtaaata
     6601 tggaattttc aaagaaaaca cgtgaattgt caattaaaaa aatgcaggaa cgtaccctgg
     6661 acctcttgat tatcggtgga ggaatcacag gagctggtgt agccttgcag gcggcagcta
     6721 gcggtcttga gactggtttg attgaaatgc aagactttgc agaaggaaca tctagtcgtt
     6781 caacaaaatt ggttcacgga ggacttcgtt acctcaaaca attcgacgta gaagtggtct
     6841 cagatacggt ttctgaacgt gcagtggttc aacaaatcgc tccacacatt ccaaaatcag
     6901 atccaatgct cttaccagtt tacgatgaag atggagcaac ctttagcctc ttccgtctta
     6961 aagtagccat ggacttgtac gacctcttgg caggtgttag caacacacca actgcgaaca
     7021 aggttttgag caaggatcaa gtcttggaac gccagccaaa cttgaagaag gaaggcttgg
     7081 taggaggtgg agtgtatctt gacttccgta acaacgatgc gcgtctcgtg attgaaaaca
     7141 tcaaacgtgc caaccaagac ggtgccctca ttgccaacca cgtgaaggca gaaggcttcc
     7201 tttttgacga aagtggcaag attacaggtg ttgtagctcg tgatctcttg acagaccaag
     7261 tatttgaaat caaggcccgt ctggttatta atacaacagg tccttggagt gataaagtac
     7321 gtaatttgtc taataaggga acgcaattct cacaaatgcg cccaactaag ggagttcact
     7381 tggtagtgga ttcaagcaaa atcaaggttt cacagccagt ttactttgac acaggtttgg
     7441 gtgacggtcg tatggtcttt gttctcccac gtgaaaacaa gacttacttt ggtacaactg
     7501 atacagacta cacaggtgat ttggagcatc caaaagtaac tcaagaagat gtagattatc
     7561 tacttggcat tgtcaacaac cgctttccag aatccaacat caccattgat gatatcgaaa
     7621 gcagctgggc aggtcttcgt ccattgattg cagggaacag tgcctctgac tataatggtg
     7681 gaaataacgg taccatcagt gatgaaagct ttgacaactt gattgcgact gttgaatctt
     7741 atctctccaa agaaaaaaca cgtgaagatg ttgagtctgc tgtcagcaag cttgaaagta
     7801 gcacatctga gaaatatttg gatccatctg cagtttctcg tgggtctagc ttggaccgtg
     7861 atgacaatgg tcttttgact cttgctggcg gtaaaatcac agactaccgt aagatggctg
     7921 aaggagctat ggagcgcgtg gttgacatcc tcaaagcaga atttgaccgt agctttaaat
     7981 taatcaattc taaaacttac cctgtttcag gtggagaatt gaacccagca aatgtggatt
     8041 cagaaatcga agcctttgcg caacttggag tatcacgtgg tttggatagc aaggaagctc
     8101 actatctggc aaatctttac ggttcaaatg caccgaaagt ctttgcactt gctcacagct
     8161 tggaacaagc gccaggactc agcttggcag ataccttgtc ccttcactat gcaatgcgca
     8221 acgagttggc tcttagccca gttgacttcc ttcttcgtcg taccaaccat atgctcttta
     8281 tgcgtgatag cttggatagc atcgttgagc cagttttgga tgaaatggga cgattctatg
     8341 actggacaga agaagaaaaa gcaacttacc gtgctgatgt cgaagcagct ctcgctaaca
     8401 acgatttagc agaattaaaa aattaagaaa aaataaaaga ggtggagggc agcattcctt
     8461 gtcgcccgtc ccttcttttt aatggagaca gaaagatgat gaatgaatta tttggagaat
     8521 ttctagggac tttaatcctg attcttctag gaaatggtgt tgttgcaggt gtggttcttc
     8581 ctaaaaccaa gagcaatagc tcaggttgga ttgtgattac tatgggttgg gggattgcag
     8641 ttgcggttgc agtctttgta tctggcaagc tcagtccagc tcatttaaat ccagctgtga
     8701 cagttggtgt agctttaaaa ggtgacttgc cttgggcttc cgttttgcct tatatcttag
     8761 cccagttcgc aggggccatg ctgggtcaga ttttggtttg gttgcaattc aaacctcact
     8821 atgaggcaga agaaaatgca ggcaatatcc tggcaacctt cagtactgga ccagccatca
     8881 aggatactgt atcaaacttg attagcgaaa tccttggaac ctttgttttg gtgttgacaa
     8941 tctttgcttt gggtctttac gattttcagg caggtatcgg aacctttgca gtgggaactt
     9001 tgattgtcgg tatcggtcta tcactaggtg ggacaacagg ttatgccttg aacccagctc
     9061 gtgaccttgg acctcgtatc atgcacagca tcttgccaat tccaaacaag ggagacggag
     9121 actggtctta cgcttggatt cctgttgtag gccctgttat cggagcagcc ttggcagtgc
     9181 ttgtattctc acttttctag tttatactct tcgaaaatca aattcaaacc acgtcagcgt
     9241 cgccttaccg tactcaagta cagcttgcgg ctagcttcct agtttgctct ttgattttca
     9301 ttgagtatta gaaaacaatt attttgatag agcttgggca agagcccaat ttcagcaaaa
     9361 aatgaagtaa atcttctcat aataaaacgc atcatatcaa gcacgaaaat tccacgaggt
     9421 caactacagt cagaaagctg aacaacaagc caaaacgccc aaaaaaggcg gcaaaaagca
     9481 agcacctgca agcaacgtgc cgaaatggtc aaatcctgat tatgtcaacg aattagaccc
     9541 aaaaatcgtt gatatgctag tagaatttca caagtcacaa ggcactttgg aaactcccga
     9601 ggcgcaagca gaaatcgccc aaaaacgtga agaaatcgag caaaggagag ctgagcttga
     9661 ggataaaaaa caagagcttt tgaaccgctt gaacaaatag agtttcgcaa gtattatgct
     9721 tacaaattac ttgagcaatt aactaaaata taaaccctgc ctagatataa aggcagggtt
     9781 tatattttag aaattcacgt aggttgttac ggtttttaca tacccagtat agtttgagtt
     9841 tctatagtat tcagtgataa acttccattt tctttgagca acatggatat aagtacttgt
     9901 tatgtagtat ggatatgggc tttgtgaatc caagtaagac tgataagctt gtataccaaa
     9961 atatgctcca ccaattattg caccccatgg accccccaat aaagcaccta tcctaccaat
    10021 catataactg attccagcac cagtcatgaa gttagcgaat gtgttagctt gtttattccc
    10081 atgtattgtg ttgacgtaat tccaaacatt aggatcgtat gatctaaaag atatatttag
    10141 gtcgatttca ttcttttgat aagccatata aaatgcccca ttgatataga cgccgtcagc
    10201 acgtcgttca atagtgtcta cacttccatc tggattgaca acctcaagaa cttcatcgct
    10261 taaaatattt acttgcgtat ctccgaaccg cactgatgag ccattctcaa actgagcctc
    10321 accagataca actttagagt ttgccgataa gctatcatca gcaaaaacaa caagcgacgg
    10381 ggaaatgcta gacatacaga aaacagacat aactagcaaa cacatgcatt taaacatctt
    10441 agacataacg gaaactcttt tgtatttttg atttttttca acttttatta tacaataaaa
    10501 ccaaataaaa agaaagcggt aacaatatgc ttaatgcgaa aattttttat atatttttat
    10561 gtttgatcgt tatcgaaact acaggcttgt tgttgttgaa aagaggtctc gaaatgggtt
    10621 atttagacac agaagctatt atcctcgcag ttttttcatt tgctttttac aacctatgtt
    10681 cattcgcttg ggtctgctct acaataaaaa ataaatagac gtattttcaa aaaaaaaaat
    10741 gcatatttat attagcaaaa tgacgattta aatcgtcgtt tttttgtagt acgacgggca
    10801 tgtcgtatat ctgaggtgta agtcctcggt gggcacctgc taccggtgaa cccaatagcg
    10861 attcccaagc ctgactatcg tgaggtagcg gattaaaacg gtctggggat agaccgtttt
    10921 aagtctgacg caggaaataa gaatcgtcag aggaagggat agcgaaatcg tggctctacg
    10981 aacaggaacg tgatagtaag gcgtatatag cggataagga ggcttcaaac tctaaagtcc
    11041 aaaaaggtag tcgtaaccta tatgtgtaaa tcacgagagt aattgaattc ggactaaggt
    11101 ttgtgtgaaa aagataaatc tttctagagt ctaaagactc tgcgtcagat ttcctatttt
    11161 cactgtaacc ttttaacgtc ctcatatctt gtataaacga ggaaagatgt acgacttatc
    11221 ccgtgaggtt tcatgagcgc tgaaagcgta gtaacaacga atcatgagaa gtcagccgag
    11281 cccatagtag tgaggaaact tctgtagtgg aagtggagcg aaggggtgaa tactcaaaca
    11341 gtctggggag agactgtttg aggtctgtcg ctagaaagag aaaacgacag atcgaagtaa
    11401 tcctacttca cttgtgtctg taaaatgagt ggtctgatag aactggactt tgacacgtaa
    11461 tgactagatg aaggttcacc aatataagat gtccctcagg caccaaaaca agaaaggaat
    11521 acgcacatgt caaaactgct agataagata ttatcacgcg aaaatatgct ggaagcctac
    11581 aatcaagtaa aatccaataa aggctcagct gggattgatg gaatgactat cgaagagatg
    11641 gataattatc tcagacaaaa ctggcgcttg actaaggaac tgataaaaca gagaaaatat
    11701 aagcctcaac caattcttaa agttgagata cctaaaccag acggaggcat ccgtcaacta
    11761 ggaatttcaa cagttatgga tagaatgatt caacaggcca ttgtccaagt catgagcccc
    11821 atttgtgaac cccatttctc agatacgagt tatggtttca gaccaaatag gtcatgtgaa
    11881 aaagccatca tgaagctctt agaatactta aatgacggct atgagtagat agtggatata
    11941 gacctagaga aatttttcga tacagttcct caagatagat tgatgtcctt agtacataac
    12001 attatcgaag acggagatac ggaatccttg attcgtaagt atcttcattc aggtgttatc
    12061 attaatggtc aacgttataa aacactagtt ggtacaccac agggaggaaa tttatctcct
    12121 ctcttatcca atatcatgct taatgaattg gacaaggaat tagaaaagag gggacttcga
    12181 tttgtgcgct acgcagatga ttgtgtgata cggtcggaag cgaggcagcc gctaagcgtg
    12241 tgatgtattc agtcagtcgt tttattgaga aacggctagg tttgaaagta aacatgacca
    12301 agactaagat taccagacca agagagttga aatatctagg ttttgggttc tggaaatcat
    12361 cagatggttg gaaaagccgt ccacatcaag atagtgttcg gagatttaag tttaaattga
    12421 agaaactaac acagaggaaa tggagtatag acctaacaag acgtattgag caactgaatt
    12481 tgtctattcg aggatggata aactatttct cattgggaaa tatgaaaagt atagtcgcca
    12541 gcatagatga gcgcttgcgt actcgcctac gagtgattat ctggaagcaa tggaagaaga
    12601 aatcgagacg attatgggga ttgcttaagt taggagttcc taaatggata gcagataagg
    12661 tatctggctg gggcgaccat tatcaattag tagctcagaa gtcggtactt aaacgtgcta
    12721 tatcaaaacc agtccttgaa aaacgtggac tggtttcgtg tttggattat taccttgaac
    12781 gacatgcgtt aaaagttagt tgaaccgccg tatgccgaac ggcacgtacg gtggtgtgag
    12841 aggggctaga gattatcccc tactcgatta actcccctga aatttatttt aattatgcaa
    12901 atttcacgta tttttgatgc tgagacgacg atcctgggaa cttttcagat atttttttga
    12961 ctatctaaat ctatcattag aaaagcttag agcgccaaag gatttgagcg tttttctgat
    13021 ttttaagact ttttcccagt ctctttttcg attgaagatg taattattct actaactaac
    13081 taacttctta gtactagcca acaacgataa tcataattcc tcctaaaatt aggaataata
    13141 aaggcaatag tttttgtttt ttcatgtaaa aaacctcact tttgttttct gctattttat
    13201 gctaaaatat taaaaatcaa atttaattcc aaagtttgta actaaagggg gagcgctaca
    13261 tgtctaattc atttgtcaag ttgttagtct ctcaattatt tgcaaattta gcagatattt
    13321 tctttagagt aacaatcatt gctaacatat acattatttc aaaatcagta attgccacat
    13381 cactagttcc tatcttaata ggaatatcct cttttgttgc gagtctttta gttccgttgg
    13441 ttactaaaag gttagcgcta aatagggttt tatctttatc tcaatttgga aagactatat
    13501 tattggcgat actggtagga atgtttaccg taatgcaatc cgtagcgcct ttggtgacct
    13561 atctatttgt tgttgcaatt tccatactag atggttttgc agcacccgtt tcctatgcta
    13621 ttgtgccacg ctatgcgacc gatttgggta aggctaattc agccttatca atgactggtg
    13681 aagctgttca attgataggt tggggattag gtggactctt gtttgcaaca attggtctgt
    13741 tacctaccac gtttatcatt ttagtcttgt atatcatttc tagctttctg atgttatttc
    13801 ttcctaacgc tgaagtggag gtgttagagt cagaaactaa tcttgaaatt ttgctcaaag
    13861 gttggaagtt agttgctaga aatcctagat taagactttt tgtatcagca aatttattgg
    13921 aaattttttc aaatacgatt tgggtttctt ccattatact tgtttttgta acggagttat
    13981 taaataaaac ggaaagttac tggggatatt ctaatacagc atactctatt ggtattataa
    14041 ttagtggctt aattgctttt aggctatctg aaaagttccg tgctgctaaa tgggaaagta
    14101 ttctttttcc tcttgtagct atggcaatag tgacactgac tattctatat tttccaaacg
    14161 cacagatgtt tttattattt tcagctttag ttggtatgtt atcgcaacta aaagaagttc
    14221 ctgaaagtgt atttcttcag gaaacagtag aggaaaatca tttagttaat gtctattccg
    14281 ttcttgaagt gatttctaca ttagcatttt cagtatttgt tttgctaatg agctatatta
    14341 ctgagagttt tggtattagc atcagttttt ggctatcagc catttgtctg atgatagaag
    14401 ctattttaat ttatatcaga cgagattatt ttaaatgaga gagtggttaa aaagtcttta
    14461 aaaatcagaa aagcgcatag tatcaggtgt tgaaaaaact tgatattgtg tgttttatta
    14521 tgggaagatt tacttcattg tctcctgaaa tggagttttg aagcaggatc actctatttg
    14581 aaaatataca tagaaaaacc gcataatcct taaaataagc tgaaatctat tggaaacata
    14641 cggtaaacaa ttgaaaagat actatataaa gtggtacaat ggtagaaaaa taagctagta
    14701 agaatcactc ttattttagc gaaaaattac agaaatgaat ggtttttctt gatgaaacag
    14761 agaaaagaat tgtacctctt tcttggtcgg acagccttgt attttcttat ctttctaggg
    14821 ctgctttact tctttagcta tcttggtcag ggtcaaggaa gctttatcta taatgaattt
    14881 taacttgaag gagaatccct attgtgtcaa ataaaccaat agcagatatg attgaaacca
    14941 ttgagcattt tgctcagaca cagcctagct atcctgtcta taatgttttg gggcaggaac
    15001 acacttatgg cgatttaaag gctgattcgg atagtttggc tgcagtcatt gaccaactag
    15061 gcttgcctga gaagtctcct gtggttgttt ttggtggcca agaatatgaa atgttggcaa
    15121 cctttgtagc gctgactaag tcaggtcatg cctacattcc aattgatagc cattcggcct
    15181 tggagcgagt ttcagctatt ttagaagtag cagagccaag cttgattatt gccatttcag
    15241 cctttccctt ggagcaggtt tctacaccaa tgataaatct agctcaggtt caagaagcct
    15301 ttgcccaagg gaacaactat gaaatcacgc atccagtcaa gggagatgat aattactaca
    15361 ttatctttac ttctggtacg actggtaagc ctaagggagt gcagatttca catgataatc
    15421 tcctcagctt tacaaactgg atgattacgg ataaggaatt tgcgacaccg agtcgtccgc
    15481 aaatgctggc acagccacct tattcttttg acttgtctgt catgtattgg gcaccgacct
    15541 tggcacttgg tggtacgctt ttcactcttc cttcagtcat cactcaggac tttaagcaac
    15601 tctttgcggc tatcttttca ttgccaatcg ctatctggac atcaacacca tcctttgcag
    15661 atatggccat gttgtctgaa tacttcaaca gtgagaaaat gcctggaatc acgcatttct
    15721 actttgatgg tgaagaattg acggtcaaaa cagctcaaaa actgcgcgag cgtttcccaa
    15781 atgcccgtat catcaatgct tacggcccaa cagaagcgac agtagctctg tcagcagttg
    15841 ccgtgacaga cgagatgtta gcgactctca aacgcctacc aatcggctat actaaggctg
    15901 attctccaac ctttatcatt gacgaggaag gaaataaact gccaaatggt gagcagggag
    15961 aaatcattgt ttctgggcca gctgtttcaa aaggttatat gaacaatcct gaaaaaacag
    16021 cagaagcctt ctttgagttt gaaggtctgc cagcctatca cacaggcgat gtgggaacca
    16081 tgacagatga gggcttgctt ctctacggcg gacgcatgga cttccagatt aagtttaacg
    16141 gttaccgcat tgagttagaa gatgtctctc aaaacctcaa caagtctcgc tttatcgaat
    16201 ctgctgtcgc agtaccgcgc tataacaagg accacaaggt acaaaatcta ttggcttatg
    16261 tcatcttaaa agacggtgtt cgtgagcagt ttgagcgaga tatcgatatt accaaggcca
    16321 tcaaggaaga cctgacagac atcatgatgt cctatatgat gccatctaaa ttcctttacc
    16381 gagacagttt gccactaact ccaaatggaa agattgacat caaaggattg attaacgagg
    16441 tgaataagag atgatggagt tttttcaaca gcttcctcat ttagagccat atggcaatcc
    16501 tcagtatttt gtttatgtga ttgctgcaac cttgcccatc tttataggtc tctttttcaa
    16561 gaaacgcttt gcctggtatg aagtgttggt aagtctcttc tttattgtca ccatgttggt
    16621 gggtggaaag accaatcaac tagctgcctt gggtatttac ctttgctggg aaatattgct
    16681 cctgcttttc tacaagcatt atcgaaaaag caaggatggc aagtgggtct tctacttagt
    16741 tagttttctg tccctacttc cgattatctt tgtcaaggtg caaccagcta tcaatggaac
    16801 gcagtctttg cttgggttct tgggaatttc ttaccttacc tttcgttcgg ttggaattgt
    16861 catcgagctg agagatggag tgattaagga ttttaccctc tgggaattcc tccgtttcct
    16921 tctcttcatg ccaactttct cgagtggtcc aatcgatcgc tttaagcgat ttaatgaaaa
    16981 ttatcaggct attcctgagc gagatgagtt gatggatatg ctggatgaat ctgtccgcta
    17041 tatcatgtgg ggctttttgt ataagtttat cctagctcat gttttaggag agaccttact
    17101 acctcctctg aagaatttag ccttgcagtc aggtggcttc tttaatctct atgccttggc
    17161 agttatgtat acttttggtc tggaactctt ctttgacttt gcaggttatt ctatgtttgc
    17221 tttggccatc tcaaacttga tgggaatccg tagccctatc aactttaaca agcccttttt
    17281 atcaagggat ttaaaggagt tttggaatcg ctggcatatg agtctgtcct tctggttccg
    17341 tgactttgtc tttatgcgaa tggtgatggt gttaaccaga aagaaagtct ttaaaaatcg
    17401 taatgtaacc tcaagcatgg cctacattgt aaatatgctg attatgggat tttggcatgg
    17461 cgtgacctgg tactatatcg cctatggact ctttcatgga ctaggcttgg tcatcaatga
    17521 tgcctgggtt cgcaagaaaa aaacgctcaa taaggaacgg aaaaaagcag ggaaggctgc
    17581 cctacctgag aatcgctgga ttcagtttct tggcatggtt gtcactttcc atgttgtcat
    17641 gttgtcattc ttaatctttt ctggattctt gaataatcta tggtttaaaa aataaaagga
    17701 aataaaatat ggatatcaaa tcagaagtta ttgaaattat tgatgagttg tttatggaag
    17761 atgtttctga catgatggat gaagatcttt ttgatgcagg tgtcttggat agtatgggaa
    17821 cggttgagtt gattgtggag attgagaacc gttttgacat tcgtgtccct gtaacagagt
    17881 ttggtcgtga cgactggaat acagctaata aaatcatagc tggtattgtg gagctacaaa
    17941 atgcttaaac gcttatggat gatcttcgga ccggtcttga tcgctggttt gttggttttt
    18001 ctgctcattt tcttttatcc tactgagatg catcataatc taggagctga aaagcgttca
    18061 gcagtggcta ctactatcga tagttttaag gagcgaagtc aaaaagtcag agcactatct
    18121 gatccaaatg tgcgttttgt tcccttcttt ggctctagtg aatggcttcg ttttgacggt
    18181 gctcatcctg cgatattagc tgagaaatac aatcgttcct accgtcctta tcttttagga
    18241 caggggggag ctgcatcgct taaccaatat tttggaatgc aacagatgtt accacagctg
    18301 gagaataaac aagttgtgta tgttatctca cctcagtggt tcagtaaaaa tggctatgat
    18361 ccagcagcct tccagcagta ttttaatgga gaccagttga ctagttttct gaaacatcaa
    18421 tctggggatc aggctagtca atatgcagcg actcgcttac tgcaacagtt cccaaacgta
    18481 gctatgaagg acctggttca gaagttggca agtaaagaag aattgtcgac agcagacaat
    18541 gaaatgattg aattattggc tcgttttaat gaacgccaag cctccttttt tggtcagttt
    18601 tcggttagag gctatgttaa ctacgataag catgtagcta agtatttaaa aatcttgcca
    18661 gaccagtttt cttatcaagc aatagaagat gttgtcaaag cagatgctga aaaaaatact
    18721 tccaataatg agatgggaat ggaaaattat ttctataatg agcagatcaa gaaggatttg
    18781 aagaaattaa aggattctca gaaaagcttt acctatctca agtcgccaga gtataatgac
    18841 ttgcagttgg ttttaacaca gttttctaaa tctaaggtaa acccgatttt tatcattcca
    18901 cctgttaata aaaaatggat ggactatgct ggtctacgag aggatatgta ccaacaaacg
    18961 gtgcagaaga ttcgctacca gttagaaagt caaggtttta ccaatatagc agatttttct
    19021 aaggacggcg gggagccttt ctttatgaag gacaccattc accttggttg gttgggttgg
    19081 ttggcttttg acaaggcagt tgatcctttc ctatccaatc ccacaccagc tccgacttac
    19141 catctgaatg agcgcttttt cagcaaagat tgggcgactt atgatggaga tgtcaaagaa
    19201 tttcaataga taatataata tagaagagtt caagaatgaa actagttttc acgttttttc
    19261 ttgactcttt ttttgcttgt gatataatta actggtaaac aaaatgtcaa agaatagtat
    19321 ttttctctta aaaaagagaa gtctaaaagg aaaggagtca gatatgagac agctagcaaa
    19381 ggatatcaat gcttttttga atgaggtgat tttgcaggcg gaaaatcagc atgaaatcct
    19441 aataggtcat tgcactagcg aggtggccct gaccaatact caggagcata tccttatgct
    19501 cttgtcagag gaatctttaa caaattcaga attggcccgt cgtctcaatg tcagtcaggc
    19561 ggcagttacc aaggccatta agtctttggt caaggaaggg atgttggaaa catctaaaga
    19621 ttctaaagat gcgcgtgtga ttttttatca gttgactgac ttggctcgtc caatcgctga
    19681 ggagcaccac catcaccatg agcatacact tttaacctat gaacaagtgg cgactcaatt
    19741 tactccaaat gaacaaaaag tgattcagcg gtttttgact gctttagtag gagaaatcaa
    19801 ataatgagat atattacggt agaggatttg tccttctatt atgataagga gcctgttctt
    19861 gaacatatca attatagtgt tgatagtggg gaatttgtta ccttgactgg ggaaaatgga
    19921 gcggctaaga cgacgctcat caaggctagt cttggaattc tgcaaccacg cattggaaag
    19981 gtggctattt caaagacaaa tacgcaaggt aagaaattga gaatagccta tcttcctcaa
    20041 caaattgcca gttttaatgc tggttttcca agtacggtct atgaatttgt caagtcgggt
    20101 cgctatccga gaaaaggctg gttccgtcgt ttgaatgctc atgatgagga gcatatcaag
    20161 gctagtctgg actcagttgg catgtgggaa catcgagaca aacgcttggg gtctctatct
    20221 gggggacaaa agcagcgagc ggtaattgcg cgtatgtttg cttctgaccc tgatgtgttt
    20281 atcctagacg agccgacaac ggggatggat gcaggaagta aaaatgaatt ttacgaactc
    20341 atgcaccaca gcgcccatca tcatggcaag gctgttttga tgattaccca tgaccctgaa
    20401 gaagttaagg attatgcgga tcgcaatatt catctagtcc gtaaccaaga ctcgccatgg
    20461 cgttgtttca atgttcatga gaatggccag gaggtgggcc atgcttagtt tattatctta
    20521 cgactttata cagcgcgcct ttttggcggt tattgctatg agtcttttct caccggtatt
    20581 gggaaccttc ctcatcttgc gtcgtcagag tttgatgagt gataccctta gccacgtctc
    20641 actttcaggt gtagcctttg gtctggtttt ggggatttct ccaactgttt ctactattgc
    20701 cattgtcttg attgcggcgg tctttctgga gtatctccgt acggtttaca agagctttat
    20761 ggaaatcggg acagctatcc tcatgtcaac aggtctggct gtttctctga ttgtcatgag
    20821 caagggtaaa agctcgagtt caatgagttt ggaccaatat ctctttggtt cgatcgtgac
    20881 tatcagtgaa gaacaggtca tttccctctt tgttattgcg gcggttgttt tgattttgac
    20941 ctttctcttt cttcgtccta tgtatatctt aacttttgac gaagatacgg cctttgtgga
    21001 tggcttgcca gttcgtacca tgtccattct ttttaacatg gtgacagggg tggctattgc
    21061 ccttatgatt cctgcagcag gagctcttct ggtatcgacc attatggtct tgccagctag
    21121 tattgccctg cgtctgggga aaaactttaa atcggttatg ctgcttgcca gtgcgattgg
    21181 ctttttggga atggtagcag gactttacat ttcctactat gcagaaacac ctgcaagtgc
    21241 aagtattacc attatttttg taactgtctt tatactaatc agtttagtaa gacgttttat
    21301 caaataggag acgaacgtga aaaaaattag cttattacta gccagtctat gtgccttgtt
    21361 tttagtggct tgttccaatc aaaaacaggc agatggtaaa ctcaatatcg tgacaacctt
    21421 ttaccctgtc tatgaattta ccaagcaagt tgcaggagat acggctaatg tagaactcct
    21481 aatcggtgct gggacagaac ctcatgaata cgaaccatct gccaaggcag ttgccaaaat
    21541 ccaagatgca gataccttcg tttatgaaaa tgaaaacatg gaaacatggg tacctaaatt
    21601 gctagatacc ttggataaga aaaaagtgaa aaccatcaag gcgacaggcg atatgttgct
    21661 cttgccaggt ggcgaggaag aagagggaga ccatgaccat ggagaagaag gtcatcacca
    21721 tgagtttgac ccccatgttt ggttatcacc agttcgtgcc attaaactag tagagcacat
    21781 ccgcgacagc ttgtcagcag attatcctga taaaaaagag acctttgaga agaatgcagc
    21841 tgcctatatc gaaaaattgc aatccttgga taaggcttac gcagaaggct tgtctcaagc
    21901 aaaacaaaag agctttgtga ctcaacacgc agcctttaac tatcttgcct tggactatgg
    21961 actcaaacaa gtcgcaatct caggcctttc tccagatgca gagccatcag ctgctcgctt
    22021 ggcagaattg acagagtatg tcaagaaaaa taaaatcgcc tatatctatt ttgaagaaaa
    22081 tgcctcacaa gcccttgcta acacactttc aaaagaagca ggtgtcaaaa ctgatgtcct
    22141 caatccttta gaaagtctga cagaagagga caccaaggct ggagaaaact acatttccgt
    22201 gatggagaaa aacctcaagg ctttgaaaca aacaacagac caagaaggcc cagcaatcga
    22261 acctgaaaag gcagaggata ccaagacagt ccaaaatggt tacttcgagg atgcagctgt
    22321 caaggaccgc accttgagtg actatgcagg taactggcaa tcagtttatc ctttccttga
    22381 agacggcacg tttgaccaag tctttgacta caaggctaag ttgactggta agatgaccca
    22441 ggctgagtac aaggcttact atacaaaagg ctatcagaca gatgtgacta agattaacat
    22501 tactgataat actatggaat ttgttcaagg tggacaaagc aagaaataca cttacaagta
    22561 tgtcggtaag aaagttttga cttacaagaa aggcaatcgt ggcgtgcgtt tcctctttga
    22621 agccacagat gctgacgctg gacaattcaa gtatgttcag tttagtgacc acaatatcgc
    22681 cccagttaag gcagaacatt tccatatctt ttttggaggt acaagccaag aaactctatt
    22741 tgaagaaatg gataattggc caacctacta cccagataac ctatctggcc aagaaatcgc
    22801 ccaagaaatg ttggcgcatt gatgagaaat cgactagttc ataagagcta gtcggttttt
    22861 tgatactaga tggctaactt cattatagac ctttcagaac taagggaacc tgaaaaaata
    22921 aataaaactc ttgaaatatc tgggcttata tgctagaatg tagttagtgt ataaggttca
    22981 ttagaacacc aaagccccta actagtgcca tagttaaggg cttttttgac gtatcttagc
    23041 gatttaacgg gtgtttatag gctagtcatt cggtctactt cttaccatct agccatttgc
    23101 agacgaaata caagataatt ccagctataa catctgctat aatagtaaga gcaagctctg
    23161 gtataaggct cattagtttc acctcctctc acgaacccat aggaacgtaa tcggtaaccg
    23221 atgacaaaaa tagtatacca cactaaattt agatcatcaa cctcacttaa tacttgaact
    23281 attgtagatt atatgctaaa atgtagttaa ttgataaggt tcattagaac accaaagccc
    23341 ctaactatgg ccgtagttaa gggctttttt gacgtatctt agcgatttaa cgggtgttta
    23401 taggctagtc agtcggtcta ctccttacta tctagccatc tgcagacgaa atacaagata
    23461 attccagcta taacatccgc tataatagta agagcgagct ctatgataag gctcattagt
    23521 ttcacctcct ctcacgaacc cataggaacg taatcggtaa ccgatgacaa aaatagtata
    23581 ccacaataca tttagatcat caaggtcact taattcttga aatatagggt ctaggcgaca
    23641 aagtctttaa aatcagaaaa acgcataata tcaggtgtgc aaaaacttga tactatgcgt
    23701 tttattgtgg gaaggtttac tccattttct cctgaaattg agtttttgtc cagcctctgt
    23761 ttttagggtt gctgagaaaa taatgtcatg tggtgaatat ttgtaaatca gtcagcagac
    23821 agaacgaata ctcttcgaaa atctcttcac atcatgtcag cttcgtcttt ccgtatatat
    23881 gtgactgact tcatcagttc tatctacaac ctcaaaacag tgtttcgagc tgacttcatc
    23941 agttttcaaa tctgtacttt gagcaagctg agactagctt cctatttgat tttcattgaa
    24001 tatcagaaac ccattctcca tcaaataatt cgactgcgtc taataatttt tgatctggca
    24061 cggtgtctga aataaaggtt gtgtatttgg agaggggatt aattttaaaa aatccagtct
    24121 tgtaaaattt agaactatca atcagtaaga tggtttcatg ggctttgtca ataatattct
    24181 tttttgaaat agcttggctg agagaagctt cataaacata ttggtcatca atacctcttg
    24241 ctgaacaaaa tgctaaatcg atattaaaat gatctaataa agaattttcc ttatcatagt
    24301 tgaccacgga acaggattga tgtttgacct cgccagatgt gataaagatt ttggagctat
    24361 ctttaacagt ttcagatagg gtttgtgcag tatgtaaacc atttgtaaaa ataatcaaat
    24421 tatcaagttc agaaagatag ggacagagtt cgtagacagt cgtactggaa tccagataga
    24481 tacacatacc agaccgaata aagtctttag cgagactagc gattagtctt ttttgcctag
    24541 tactttctcc ttcacgtatt tgatgagaaa gttcaattgt gttcatagag gacagggtca
    24601 cgtatccgtg ctttcttttg ataagacctt gattttctaa gaaaattaaa tcacgacgta
    24661 aggtacttgt gctggagaaa gtgatttctg ccagctcttt tacggcaatt ctttttttct
    24721 ttttaataat ttcaatcaat tcaagtacac gttcatcttt tatcataagc tcctcctaat
    24781 ttatcatttc aactatatta tagcacaaat tggaggaatt tgaattattt ttatgaatat
    24841 tgggttaaca tttaaacatt attcaagtaa gcgttcacat attgaaaaaa taaaacgtgg
    24901 ggattataat aaagttaatc aaggacgaag agagaagaaa aatggaagcg gttttagcaa
    24961 tagatttagg tgcgacttct ggaagagcaa tcgttggtta cctttctgaa aataaactag
    25021 taatggaaga aataaatcgc ttttctaatc tacctattag agtaaaaggg catttatctt
    25081 gggatattga ctttctacta gctaaaattc ttgaaagtat ccgcttggct aatactagtt
    25141 acaagatttt atctatcggt attgacacat ggggagttga ttttggactg attgataatg
    25201 aaggtaagct gttattacaa cctgttcatt atcgtgatga aagaacaaag ggagtgttaa
    25261 aggaaatatc tgaaatgact gaattagaaa aactgtattc agagacagga aatcagatta
    25321 tggagataaa taccttgttt caactcttta aggcacgtca agaatctcct gactctttct
    25381 ataagaccaa taagattctt ttaatgccag atttgtttaa ttatctcttg acaggtaagt
    25441 ttgctacaga aaaaagcatt gcttcaacaa ctcaattatt tgatcctagg agtcaaaatt
    25501 ggaatcagaa tatcttaaaa ctatttgaat tggattcaac tttacttcct gaaattgttt
    25561 cagagggaaa tgtccttgga aggataaaag aggagtatgg tttaggcgat attcctgttg
    25621 tgaatgtttg tagtcatgat acagcaagcg cgattgtctc agtacctaag acagaagata
    25681 gtttgtttat ttcatcaggg acttggtctt tggttggagt ggagctttct tcacctattc
    25741 ttagtaccga atccttcagt tatggattta caaatgaagt cggtaaagat ggagtgatta
    25801 catttctgaa gaattgtaca gggttgtgga tcatagagga actaagacgt tcatttgaac
    25861 gaagagggaa agcctattct tttgatgata ttaggacaat ggtggagaaa gaaaaagaaa
    25921 atcttcctct gattgatact gaatcaactg aatttgcaac agaatctgat atgcacaaga
    25981 ctttgacaga atatctagct tatcatcatg aaactggaga gtggacagat ggacaactat
    26041 ttaagatcgt ttatgaaagc ctagctgaaa cgtataagga aacaatagag ttactagaag
    26101 aactaactca taaggtttat aagaggatat atgtgattgg aggaggtgct agagccagtt
    26161 actttaacca aatgattgct gatagaactg gtaaagaagt tcttacaggt tcaactgagg
    26221 ctacagctgt ggggaatatt tgtatacagc tattaagtca aggtaaaata aacgaggata
    26281 cagggttaaa ggatattatg acaaatattg ctgatacaca atattattat cctcagctat
    26341 cataaacaaa aataaaaaaa cggaggtttt gtcatgaaca aaaaaagtct tttgaaatgc
    26401 gctgttatcg ggttagtagc tacctttggt ttagcagctt gtggaacatc taaggatgca
    26461 agcggtggaa gttcttctgg taaagaagta ttagaatttt atcatgggta tcatcatagt
    26521 gaagatgaat ggcctgtagc gaagactatg cgagatttat atgataaatt tgcagaagaa
    26581 cataaagata gtggtgtaga atttaaacca actcctgtaa atggtgattt gaaggatata
    26641 atgaataaca aggttgctag tggtgaattt cctgatgtga ttgacctagc aggtaatgca
    26701 gtatcattag ctgcgattga acaaaaactt gttttagatt tgaaaccata tatcgattca
    26761 aataagcttg aaaaaaatgt tggtttgaac tataaacaaa accaaaaaga tggaaaaatt
    26821 tatacagttc acgaacagtt atttacaatg ggtctgtggt acaacaagga catatttgca
    26881 aaagcaggtg ctaagacacc tgatcagtgg aatacttggg aagattttac acaagcaatg
    26941 gctgctgtga gaaaacaaga tggagtgtat gcatttggtg ctggagaacc atctattcgc
    27001 ttgtttaata cagtcttagg aaccacagaa aatgggcgta aattattaga caaaccatta
    27061 acaaaagaag gaattgaatc taaagaattt gcagatgctt tgaagatggt catgaaagaa
    27121 attcaagcta atggttctaa gaatgctggt ggcgatgcta atgcgtattc taaagacttc
    27181 caagaaggta aatctgcagt cttctttaat ggtgtgtggg cttctggtga gatgtctaag
    27241 aacccaagtc ttgcacctgg aatctatcct gcaggtgtag caattagttc ttctggaggt
    27301 ggtatcacta tctcaagtaa gatgtctgaa gctaaacaaa aactagctct tgagtttttg
    27361 aaatatatga ctagcgatga tgtacaaaaa gtaatttttg aaaaagtagg agcaaatcct
    27421 tctaatgaaa atgtaaatgt aaaagaactt tcagaaaaga gttcagaagc tactactaaa
    27481 attttaggac aagcaatcac tcaagttaag aatgcaaaag cagttgttcc aactgtaagt
    27541 gatgtttggg gtggcgatgt acacacagct attatcaatg cattgacaga aagtgctgct
    27601 gaaaatgtag atgttgatca aaaagtaaaa tctactcaag atgtgttgaa atcattgatt
    27661 ggttaattgt tttagggagt gaaaacgaag ctcagttggc actgccaact gagcttgatt
    27721 ttacttatat agttgaaaaa aggagtttgt ttatggcaca aaaaattatg agcctacaaa
    27781 ataggaagaa tcaaaaaaga cgatttatct ttttatttct tttgccaacg ttgatttgtt
    27841 tctttctatt ttatttttat tctgtagtaa caatcttttt aacttc
//
LOCUS       DAVKHZ010000018        27684 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000018 DAVKHZ010000000
VERSION     DAVKHZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.018"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(49..276)
                     /locus_tag="VWW99_000740"
     CDS             complement(49..276)
                     /locus_tag="VWW99_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9538616.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(767..952)
                     /gene="briC"
                     /locus_tag="VWW99_000741"
     CDS             complement(767..952)
                     /gene="briC"
                     /locus_tag="VWW99_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9538617.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(1111..1878)
                     /locus_tag="VWW99_000742"
     CDS             complement(1111..1878)
                     /locus_tag="VWW99_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9538618.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(1875..2741)
                     /locus_tag="VWW99_000743"
     CDS             complement(1875..2741)
                     /locus_tag="VWW99_000743"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9538619.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2778..4145)
                     /gene="accC"
                     /locus_tag="VWW99_000744"
     CDS             complement(2778..4145)
                     /gene="accC"
                     /locus_tag="VWW99_000744"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9538620.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4157..4579)
                     /gene="fabZ"
                     /locus_tag="VWW99_000745"
     CDS             complement(4157..4579)
                     /gene="fabZ"
                     /locus_tag="VWW99_000745"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9538621.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(4576..5061)
                     /locus_tag="VWW99_000746"
     CDS             complement(4576..5061)
                     /locus_tag="VWW99_000746"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9538622.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5064..6299)
                     /gene="fabF"
                     /locus_tag="VWW99_000747"
     CDS             complement(5064..6299)
                     /gene="fabF"
                     /locus_tag="VWW99_000747"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9538623.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6321..7052)
                     /gene="fabG"
                     /locus_tag="VWW99_000748"
     CDS             complement(6321..7052)
                     /gene="fabG"
                     /locus_tag="VWW99_000748"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9538624.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQVGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(7086..8006)
                     /gene="fabD"
                     /locus_tag="VWW99_000749"
     CDS             complement(7086..8006)
                     /gene="fabD"
                     /locus_tag="VWW99_000749"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9538625.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(7999..8973)
                     /gene="fabK"
                     /locus_tag="VWW99_000750"
     CDS             complement(7999..8973)
                     /gene="fabK"
                     /locus_tag="VWW99_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9538626.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(9092..9316)
                     /locus_tag="VWW99_000751"
     CDS             complement(9092..9316)
                     /locus_tag="VWW99_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9538627.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQEK"
     gene            complement(9376..10350)
                     /locus_tag="VWW99_000752"
     CDS             complement(9376..10350)
                     /locus_tag="VWW99_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9538628.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10350..10784)
                     /locus_tag="VWW99_000753"
     CDS             complement(10350..10784)
                     /locus_tag="VWW99_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9538629.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(10878..11663)
                     /locus_tag="VWW99_000754"
     CDS             complement(10878..11663)
                     /locus_tag="VWW99_000754"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9538630.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDILSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12033..13397
                     /locus_tag="VWW99_000755"
     CDS             12033..13397
                     /locus_tag="VWW99_000755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9538631.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13400..13771
                     /locus_tag="VWW99_000756"
     CDS             13400..13771
                     /locus_tag="VWW99_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9538632.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            13985..15259
                     /gene="serS"
                     /locus_tag="VWW99_000757"
     CDS             13985..15259
                     /gene="serS"
                     /locus_tag="VWW99_000757"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9538633.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(15284..16183)
                     /locus_tag="VWW99_000758"
     CDS             complement(15284..16183)
                     /locus_tag="VWW99_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9538634.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDMLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPERRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16293..16841)
                     /locus_tag="VWW99_000759"
     CDS             complement(16293..16841)
                     /locus_tag="VWW99_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9538635.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17059..18381)
                     /locus_tag="VWW99_000760"
     CDS             complement(17059..18381)
                     /locus_tag="VWW99_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9538636.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPVITALVLSVFVAIAVFGGLKSISKVSTTVVPFMTIIYI
                     LGTLIVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18472..18560)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18724..21060)
                     /locus_tag="VWW99_000761"
     CDS             complement(18724..21060)
                     /locus_tag="VWW99_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9538637.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21164..21712)
                     /locus_tag="VWW99_000762"
     CDS             complement(21164..21712)
                     /locus_tag="VWW99_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9538638.1"
                     /translation="MISLLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(21709..22011)
                     /gene="zapA"
                     /locus_tag="VWW99_000763"
     CDS             complement(21709..22011)
                     /gene="zapA"
                     /locus_tag="VWW99_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9538639.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSTDDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22098..22979
                     /locus_tag="VWW99_000764"
     CDS             22098..22979
                     /locus_tag="VWW99_000764"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9538640.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            22983..23597
                     /gene="lepB"
                     /locus_tag="VWW99_000765"
     CDS             22983..23597
                     /gene="lepB"
                     /locus_tag="VWW99_000765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9538641.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            23677..23799
                     /locus_tag="VWW99_000766"
     CDS             23677..23799
                     /locus_tag="VWW99_000766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEU9538642.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            23876..26242
                     /locus_tag="VWW99_000767"
     CDS             23876..26242
                     /locus_tag="VWW99_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9538643.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEVPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(26290..27573)
                     /locus_tag="VWW99_000768"
     CDS             complement(26290..27573)
                     /locus_tag="VWW99_000768"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9538644.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         8056 a         6354 c         5379 g         7895 t
ORIGIN      
        1 aaagaaaaag tgtttgatat ctatcaaaca ctttttcttt gcctcccact atctaaaaaa
       61 atgataatag atataattgt aaacaaaaat ccagataggt tttgcatgat tgagaaagtt
      121 aaaaaaacta tggcagagaa tcgttaatct cagattgtcg gtagaacgat aaacaagggc
      181 aaaaaagaaa ccaatcagac tataatataa taaactaatt ggatctctgt gagatagtat
      241 caaatgacta agcccaaaga taaggcctga taagatgaca tctaaataat acttggacta
      301 gggaaagaag gtattcataa aataccctct atcaagagtc tcctcaaaaa caggatcgat
      361 gattacaggc aggacaaaag ataagatagt cgataaaaag gttggttgtc catttgaaag
      421 aaccacggta aaatactcat catgaatatt cctatgatta atcaaatgag catagcgtgc
      481 ccaaaaatta ccgagaatct gacaaaccac ataagttgca aataaggaga agacaaatga
      541 ccagttccag ctctttttct tttttaacct ccaaattaat agaaaaggaa acttcccact
      601 aatcccactg ttaaaataag agaatagaca tcagctccta accctaaaat gatcgtcaca
      661 tacaatccaa ttgtttgtgg taaataggta gatagtaaaa taataagcaa aaatattcca
      721 aattgtctta gtttctttgt gtttctcatc gtactttttt gaaagattac cctgttcgga
      781 agccgtactt ccaagcatct atataagaat taagtgcccc ttgcctcata tagggagcaa
      841 attctctata atataaccat ctactatatc catcttccca aacagcaaga ccacctgaag
      901 tttgctccaa gtcctcagtt gaaataactg taaatgtttc tgtacctgtc attgcaagta
      961 ccttcttaaa atagattgtt gtaggctcac atttatagta tatttctttt tttgtctatt
     1021 ttatagtcta tctcctcaac tggcaatttt tcgacctgaa ttgcattttt ccataaaaaa
     1081 tgagaccttt ctagtctcat ttagtcattc ttagtatttt ctaaatcgtt gatagcgttc
     1141 ttccagcaac tcttctagcg gtttttgtga aagtctagcc agctccgttt ggagttcttt
     1201 tttgacactc ttaatcagtt ctttactaga aagtcctatt tcagaaatca ccttatccac
     1261 cacgtccatt tctaacagtt catgcgaagt gattttcatc agttctgctg cttccatggc
     1321 gcgagtaccg tccttccata aaatggaagc aaagccttct ggactgagaa tggcatagat
     1381 agaattttcc agcatccaga cacggtccgc gacagctaga gccagagccc cgcctgaacc
     1441 accttcaccg ataataatgg cgataatagg aactttcagg tcactcattt ccatgagatt
     1501 gcgagcgata gcttcccctt gaccacgttc ttccgctccg acaccaggat aagcacctgc
     1561 tgtattgata aaggtcacaa ctggacggcc aaatttctca gcctgtttca tcaaccgcag
     1621 tgcctttcgg tagccttctg gatgtggttg gccaaaattc cgtttgaggt tgtcttgcaa
     1681 actcttgcct ttttggatac caaccactgt tacagcttga tctccaagcc aaccaatacc
     1741 accaacaact gcaccatcat cacgaaaaga acggtcacca tgtaattgga taaattcatc
     1801 aaaaatgcct gtcgcaaaat ccaaggttgt caagcgactc tgctcacgcg cttctctgac
     1861 tatttttgca atattcatct aggactccct ccatgcaatc tgactaggct agcaatcgta
     1921 tctggtaagt ctcttctttt gacaatagca tccacaaagc catgttctaa taggaattct
     1981 gccttttgga aatcctcagg caagctttca cgaaccgtat tttcaatcac acgacgccca
     2041 gcaaaaccaa ccaagctctg tggttcagcc agaatgatat cgccttccat agcgaaagaa
     2101 gctgtcacac caccagtcgt tggatctgtc aaaatggtca ggtaaaagag accagcattt
     2161 gaatgacgtt taaccgccgc agagatctta gccatctgca tgagactcat gattccttcc
     2221 tgcatacggg ctccaccaga ggctgtgaat aggacaactg gcaatttttc gacagtcgca
     2281 tactcaaaca aacgagtgat tttttcacct acaaccgtac ccatagaagc catgataaag
     2341 ttagaatcca taatcccaag agccacagtc tgacctttaa taagagcagt tcctgtcaca
     2401 acggcttcat gcagacctgt tttttcacgc atagatgcca gtttcttttg gtaaccaggg
     2461 aaatgcaagg gatccttgct ttcaatccct gtaaacaatt ctttgaaggt tcccatatca
     2521 atcgtcaaag ccaagcgttc ttgggcagaa atacgaaagg tatagctaca gtgcggacag
     2581 atacgttcac ttcccagatc cttctgatag atggtatgct tacagcctgg acactgggaa
     2641 aataattcat ctggaacctc tggcttagct tgaggttttt ccctaactga acgattggga
     2701 ttgattcgaa tatacttatc ttttttacta aatagagcca ttgattcccc ttttcggttt
     2761 aaactcttaa agtcatttta ttctttttct tgatatttag gtaagaaggt ttccatcaag
     2821 aaggaagtat cataatcccc agcaatgaca ttgcgatctg aaatgaggtc aagctggaaa
     2881 tctgcattgg tctgcactcc ttcaatctct aattcataga gggcacgttg cattttcatc
     2941 aaggcgtcaa aacgattttc gccgtgtact atgattttgg caatcatact atcataataa
     3001 ggcggaatgg tataacctgg ataaactgct gaatccacgc gcaagccaac tccaccactt
     3061 ggcagataga gattagtaat cttacctgga cttggagcaa agttaaaggc tgggttttct
     3121 gcattgatac gacactcgat ggcatgaccg cgtaggacaa tatcttcttg cttaacagac
     3181 aaaggctgac ctgccgcaat gcaaatctgt tccttaacga tatcaacacc tgaaacaaac
     3241 tctgttactg gatgttctac ctgaacacga gtattcatct ccatgaaata gaaattgcta
     3301 cttgcttcat caagaagaaa ttcaatggtt cctgcattct catagccaac aaactctgcc
     3361 gctcgaacag cagcagcacc tatttcatga cgcagcgttt ttccgattgc aatcgaggga
     3421 ctttcttcca aaaccttttg gttattcctt tgaagagaac aatcccgttc acccaagtga
     3481 atcacatgtc catgctcatc acctaggatt tgaacctcaa tgtgccgagc tggatagata
     3541 acccgttcta tgtacatggc accattgcca taattggcct tggcctcact agaggcagtt
     3601 tcaaaggcag aaacgaggtc atctggtttt tcaaccttac gaatcccttt accacctcca
     3661 cctgctgaag ccttgagcat aacaggatag ccaatttttt cagcaacaat caaagcttct
     3721 tcagagttat gcacttctcc atctgaacct ggtataacag gcacacctgc tttaatcatc
     3781 tgagcacgcg cattgatctt atcccccatc atatccataa catgaccaga tggaccgata
     3841 aacttgatac ctacttcttc acacatggtc gcaaatttgg aattttcact gagaaatcca
     3901 aaaccagggt gaatagcttc tgcctcagtc aagactgcag ctgatagaac tgcattaata
     3961 ttgagataag actctgttgc cttgccagga ccaatacaaa ctgcttcatc tgccaaaagc
     4021 gtatgaagag cttccttatc agcagttgaa taaaccgcta ccgtcgcaat ccccaattca
     4081 cgtgccgcac ggataatacg aaccgcaatt tcaccacgat tggcaattaa aatttttcga
     4141 aacatggaga acctccttag ttcccaattg caaaagtaag ggtaccactg gctgcaagct
     4201 tgccatccac ttcagccttt gcttcaacca cagctatggt gccacgacgt tttacaaaag
     4261 tcgctgtcat aaccaattgg tcgcctggta caacttgctt cttgaactta accttgtcca
     4321 taccagcgta aaagaccagt tttcctttat tttcaggttt tgataactcc aacacaccgg
     4381 cagtttgcgc caaggcttcc ataatcagaa cacctggcat aactgggtat tgaggaaagt
     4441 ggccgttaaa gaaaggctcg ttgatggtca catttttgat agcaacaatg gtatcctcgc
     4501 tcacttccaa gacacggtcc actagaagca taggataacg gtggggaaga gcttctttga
     4561 ttccttgaat atcgatcatt tgatacgtac caatccttta ccaaactcaa ccatttcttc
     4621 gttagagacg agaatttccg ttaccacacc atccttagga gctgggattt cattcatgac
     4681 tttcatggct tcgataatta ccaatgtttg accttttttg acactatcac caactgtaac
     4741 gaaggcaggt ttatctggtc cagcagccaa gtaaaccact ccaacaagtg gactctctac
     4801 aagatttccc tcagtagcca cacttgcttc agctggagct ggaacttctt ctgctacagt
     4861 ctctgctgga gcagatgtag gagctactgg actcggtgtt gctagaacgg gtgctggagc
     4921 gacttgagtt gcaacttcag gcacaggtct cgcttcattc ttgctaaact gcaactcatc
     4981 cgtcccattt ttataagaaa attctctcaa acttgactgg tcaaattgag tcatcaagtc
     5041 tttaatatcg tttaaattca tacttatcta ttctcccaac gtttgaaagc aagaactgca
     5101 ttgtggcctc caaaaccaaa agtatttgaa atagcgtatg gaatttcttt ctccaagcct
     5161 tgtccataaa cgacattagc ttcgatataa tctgatactt cacttgtccc agctgtcatt
     5221 ggtacaaagt tatgacgcat agcttcgatg gtgacgatag cttctactgc acccgcagcc
     5281 cccagcaaat gtcctgtaaa agacttggtt gatgatacag gtacttcctt accaagaaca
     5341 gctacgatag caccactttc tcctttttca ttggcaggag ttgacgttcc gtgagcattg
     5401 acataggcta cttgctctgg agaaatctca gcttcttcca aggctagttt gatggccttg
     5461 atagctccct gaccttctgg atgtggagaa gtcatgtggt aggcatcaca agtatttccg
     5521 taaccaacca cttcagccag gatagtagct ccacgttttt cagcgtgttc aagactttct
     5581 agaaccaaca tccctgaacc ttcacccata acaaacccat tgcgatcctt atcaaatggg
     5641 atcgaagcac gagttggatc ctctgtagta gagagagctg ttaaggcttg gaaaccagcg
     5701 atggcaaaag gtgtgataga agcttctgtt cctcccacca acatcacatc ttggaaacca
     5761 aacttaatag agcggaaggc atccccaatc gcatcatttg atgaagagca ggcagtattg
     5821 atagatttac aaacaccatt tgcaccaaaa cgcatggcta cattcccaga agccatattt
     5881 ggtaaagctt ttggaagagt cattggtttg acacgtttgg gtcctttttc atgaaggcga
     5941 agtacctgat cttcaatttc cttgattcca ccaataccag atgcaacgat aacaccaaaa
     6001 cgatccctat taagagcctc tacatcaaga ttggcatgat ttacagcctc ttgggctgca
     6061 tacaaggcat ataaagaata gttatcaaaa cggttggtat ctttttttac aaagtattta
     6121 tcgaacggaa aatcttggat ttctgccgca ttatgcacat caaagtcact atgatcaaat
     6181 tttgtaatgc caccaatgcc gattttccca gttgctaaac tattccaaaa ttcttctggt
     6241 gtatttccga ttggagatgt tactccataa cctgttacca ctactcgatt tagtttcatt
     6301 cttttcacct ctagctttcg ctacatactt aagccaccat caatggcaac cacttgacca
     6361 gttagataat cttggcctgc taaaaatact gtcaaatctg caacctgctc tgcctgccca
     6421 aattctttca tcggaatctg agctagtgta gcttccttaa tcttatctga taagatagct
     6481 gtcatatcag actcaatcat tcctggagca atcacattga ctcgtatatt ccgactagcg
     6541 acctcgcgtg ccacagactt ggtaaagcca atcaagccag ccttagaagc agcatagtta
     6601 gcttgaccaa tattccccat caaaccaaca acactagaca tattaatgat agcaccttct
     6661 ctggctttca tcatcggttt caagactgat tgtgtcatat taaaggcacc agtcagattg
     6721 accttgagca ctttttcaaa atctgcttct gtcatcttaa gcataagagt atcttgggtg
     6781 atccctgcat tgttgaccaa aacatctact gaacccagtt ctgcaatagc ttgatcaatc
     6841 atacgcttag cgtctgcaaa atctgataca tctcctgaaa tgggaaccac cttgatacca
     6901 tagtttgaaa actcagcgag caattcttct gagattgccc cacgactgtt taagacaatg
     6961 ttggctccta cttgagcaaa cttgtgggcg atggcaagac caattccacg actcgaacct
     7021 gtaataaaga tatttttatg ttctagtttc atttttttcc tttcaaaact tctacttatt
     7081 ttagtctatt tctctaaaag tgctactaaa ctcgcttgat cttccacatg agctaagtga
     7141 gcagtttgat caattttttt aacaaaacct gacaagactt tccccggtcc aatctcgata
     7201 aagttgctta tgcctgcttc ttgcatgacc ccaatacttt catagaaacg aacgggttcc
     7261 ttgacctgac gcgtcaagag ctgagcaatg tcctcttttt gcatcacagc agcttctgta
     7321 ttgccgacta ggggacaagt aaaatctgaa aaacttacct gagctagagt ttcagctagt
     7381 ttctggctag ctggctcaag gagagcggtg tgaaagggac ctgacacctt aagaggaatc
     7441 aagcgtttgg cacctgcttc ttgcaaaagt tcaaccgctc gatcaactgc aaccacttct
     7501 ccagcaatga cgatttgtgc aggtgtgtta tagttggctg gagtaaccac tccaagttca
     7561 gaagcttttt gacaggcttc ttcaatgacc tctactggcg tattgagaac tgctaccatc
     7621 ttgccagagt cagcaggagc cgcttcttcc atataggctc cacgcttagc taccaaggca
     7681 accgcatctt caaaatccaa ggcgccgctt gccaccaagg cagagtattc tccaagagac
     7741 aaaccagcaa ccatatcagg ctgatagccc ttttcttgca ataaacggta gatagcaacc
     7801 gaagtcgcta gaatggctgg ttgcgtatag cgggtctgat tgagtttgtc ctcttccgta
     7861 tcgatgagat aacgtaaatc ataaccgagc acctgactcg ctcgatcaat cgtttctttg
     7921 acaatcggat actgatcata gaaatcccgt cccatcccta gatactgggc accttgacca
     7981 gcaaataaaa aggctgtttt agtcatttct tacaactcct gcccagcgag aggcttcttc
     8041 ttgaattttc ttagcggctc cgtaatacaa atcttttagg atttcttcag ctgtttcttc
     8101 tttagaaaca agtcctgcga tttgacctgc catgacagag ccaccctcca catcaccgtg
     8161 aacaactgct ttggctaggg cacctgctcc catttgttca aagatttcta aatcaggatc
     8221 ttcctgctta aaggcatctt tttcagccag ttcaaaatct ctagtcaact gatttttaat
     8281 agcacgaaca gcatgaccaa agtgctgagc tgaaatcgta gtgtcaatat cccttgcttt
     8341 taaaattttc tccttgtagt ttggatgagc attcgactct tttgcaacta caaaccgtgt
     8401 ccccacctgt acagcctctg cacctagcat aaagccagcc gcagcacctt caccatccgc
     8461 aattcctcct gcagcaataa caggaataga tacagctgtg gctacctgtc gcaccaaggt
     8521 catggttgtt aatttaccga tatgcccccc agcttccatt ccttctgcaa taacagcgtc
     8581 tgcaccgatt ttttccatgc gtttagctaa agcgacacta ggaacaacag gaataacgat
     8641 tatcccagct tcatggaaac gttccatata cttgcttgga tttcctgctc ctgttgtgac
     8701 aactttaaca ccttcttcaa taacgagatc cacgatatct tccacaaagg gagataagag
     8761 catgatgttg accccaaagg gtttatcagt caatgatttg attttatcaa tattggcctt
     8821 gacaacttct ttcggggcat ttcccccacc gataattcct aatcctccag ccttggaaac
     8881 agcccctgcc aaatcaccat cagcaaccca ggccatccct ccttggaaaa taggatagtc
     8941 aatcttcaat aattctgtaa tacgcgtttt catagtgcct ccaaccttcc ttgcttacgt
     9001 aatagttcga tttcaccata atttgacagt caaactatta cctaaacaag agggagtggg
     9061 tttctcccta ctccttctac taatattctg cttatttttc ttgctcttca acgtaagcaa
     9121 ccaagtcacc aactgttttc aagtcatttt ctgcttcgat ttggatatca aaagcatctt
     9181 cgatttctga gattacttgg aacaagtcca atgaatctgc gtccaaatca tcaaaagttg
     9241 attcaagtgt tacttctgat gcgtcttttc caagttcttc aacgataatt tcttgtactt
     9301 tttcaaatac tgccatgata ggactccttt aaaataaata gtttttttat aacaatgtgt
     9361 tcaccacatg attacctaaa ttgtaagaat gagcgtgccc caggtcaagc ctccaccgaa
     9421 gcctgataga agaacagtct ggctaccatc taaagggatg agaccttgtt ctacacactc
     9481 tgaaagtaaa atcgggatac tggctgcact ggtattgcca tattccatca tattggctgg
     9541 aagtttggct cggtcaacac caatttttct agccatctta tccaaaatac ggtcattggc
     9601 ttgatgaagt agcagataat ccaagtctgt cacctctata ggagattcat caatagtctg
     9661 cttgatagac ttggctacat ctcgaatggc aaaatcaaag actgtgcgtc catccatctt
     9721 caaaaacgaa tctgcacttt cttgatctga aaatggagaa tgcaaacctg aatgcccata
     9781 agttaaacac tcgctgcgac ttccatcgct attgagactc tcagctaaga aatgctcttg
     9841 ctcgctagct tctaacaaga caccaccagc accatctcca aacaacacag ctgttgatcg
     9901 atccgaccaa tcgactgcct tagagagggt ttcactacca atcaccaagc ctttttgaaa
     9961 gcgaccagaa gcgataaact tttcagcagt tgaaagagca aatacaaatc cactgcaagc
    10021 cgcggttaag tcaaaagcaa aggctttatt agcgccaata ttagcttgaa cacgagcagc
    10081 tgtagagggc atcatcgaat ctggagtaat ggtagctagg atgataaaat ccagttcttt
    10141 tcctgttatt ccagcttttg ccatcagttt cttagcaacc tctgtagcca aatcactggt
    10201 agattctgtt cttgaaatat gcctttgtcg tattcccgtt cgacttgaaa tccactcatc
    10261 attggtatcc ataatctgag ccaagtcgtg atttgtaacc acttgctctg gcacataatg
    10321 agcaacctga cttatttttg caaaagccat tatttcaaat cctccaaaaa ttggtaaaga
    10381 ttagtcaagc ctttacccat gacagcaatt tcttcctcgc tcatgccatc aataattttt
    10441 tctaccatgg ccttgtggaa gcgtttatgc agtctatgaa tcaagcgacc cttctttgtc
    10501 aaatgcagat gcaccacacg acgatcctgt tctgaccgaa ctcgctcaat gtagccctta
    10561 cgctctaaat tgttcaaact tgtcgtaaca gttccaagag ttaccatcaa ctcttttgac
    10621 acttgacttg gagtcacatc tggagcctta ccgatgacat caatcgtatg catttctttg
    10681 atggagatat ccttaaaacg actacctctc aagttcactt cctcaattac aaggacattg
    10741 ttaaatatag atgttaaata ttcattgatt cgttggtagt ccatttttca tatccctcct
    10801 tcttcaaaaa acttttacaa tcaaaacatt tgacagaaag aattatatca aaattcaaag
    10861 aatgatgcaa gtatttttta ttttcctgta aatttaggtc ttcttctctc cgaatgagct
    10921 cgcactcctt ctttgaaatc ctctgtttga gctaaggatt tctgtaggtt cagttctaaa
    10981 gtagcgtaac cctgccagtc tttaaattgg ctctcccaaa ccaacttctt aatggcagca
    11041 taggaattac ttgaagcacg tcttaatttt ttaagaagct gttctctcgt cttttcaagt
    11101 ttttcagctt cagaaacgcg gtaaaccaac ccccattcta gggctttttc tgccgttaaa
    11161 gcctctcctg tcatagctaa ttgagcagca cgcgtcacac cgatactgcg gctcaagaga
    11221 tgaattcccc ctgcatctgg agccaaacca acaccaacaa aggcttggat aaacttagcc
    11281 ttatccgtcg ccaaacagaa atctgccgca acagccatat tcgctgcggc acctgcaaca
    11341 gcaccgtcaa cttccattaa aacaggtttg gctatttgct tgattttata agaaatcgta
    11401 ttgaccaact ctgcgatttt tgtcaatgat agaatatcat cctcatccac tgcccgcttc
    11461 atctctacca aatctccccc aactgagaag acctttccat tggcattgat taagataaaa
    11521 tgcacagctg gattttcttc tgccaaagtc agagcttcta aaatctcctc acacatggga
    11581 atatgaaaac catttgcgac ctcaggacgg ttcaaggtaa ggattgccaa gtcctcttca
    11641 agctgataaa taatgtgttc catcagggct cctttttatt ttataagcag tttatactct
    11701 tcgaaaatct cttcaaaccg cgtcagcttc catctgcaac ctcaaaacag tattttgagc
    11761 tgacttcgtc agtcttattt acaacctcaa agcagtgctt tgagcagtct gcggctagct
    11821 tcctagtttg ctctttgatt ttcattgagt gttaaattat ttcactgtca aagtatatct
    11881 tttttaggag cagaaagcaa gaaaaaatcg actgaaagtg tctcagccga taagaaaggc
    11941 tcgctttagg ctagaacgca tcttattttc ttcattgaaa aaagcctttc tttctgctat
    12001 aatcgtataa aatacttact ttaggagttc ttatgaaagt tgttaaattt ggaggtagtt
    12061 ctcttgcctc tgctagtcaa ttagaaaaag ttttaaacat cgtcaaaagc gattcagagc
    12121 gtcgttttgt agtcgtttct gcgcctggta aacgcaatgc tgaagatact aaggttacgg
    12181 atgccctgat taaatactac cgcgactatg ttgcgggtaa cgatattagc aagagccaaa
    12241 gctggattat cgaccgctat gctgctatgg ttagtgaatt gggactaaaa ccagctgtgc
    12301 tagaaaaaat ttctaaaagc attcacgcct tggccactct tcctattgaa gaaaatgaat
    12361 ttctctacga tactttccta gcagccggtg aaaataacaa tgccaaattg attgctgcct
    12421 actttaacca aaatggtatc gatgcacgct atatgcaccc tagagaagct gggattgtgg
    12481 tcacaagtga acctggtcac gctcgcatca ttccatcaag ttatgacaag attgaagaat
    12541 tgacaaacac caatgaagtc cttgtcattc ctggtttctt tggtgtcact aaggaaaatc
    12601 aaatctgtac tttctcacgt ggaggttctg atattacagg ttctatcatt gctgctggtg
    12661 tcaaagccga cctctatgaa aactttacgg acgttgatgg tatctttgca gcccaccctg
    12721 gtattatcca ccaaccacac tcgattcctg agttgaccta ccgtgaaatg cgcgagttgg
    12781 cctatgcagg cttctcagtc cttcatgacg aggctcttct tcctgcctac cgtggaaaaa
    12841 ttcctctggt tatcaagaat accaacaacc ctgaccatcc aggtactcgt atcgttctaa
    12901 aacacagtaa tgatgaattt ccagttgtgg gaattgctgg tgactcaggc tttgtcagta
    12961 ttaacatgtc gaaatacctc atgaaccgtg aggttggatt tggccgcaag gttctgcaaa
    13021 tcctggaaga acttaacatc ggttgggaac atatgccaac aggtatcgac gatctttcta
    13081 tcattctccg ttctcgccaa ctaactccca tcaaggaaga agaaatcctg cgtcagttgg
    13141 ttcaaaaggc taaagtagat catgcagaaa tcgaacacga cctttctatc attatgattg
    13201 ttggtgaaaa aatgaagagc cacatcggag tgactgctac tgcgacacgc gctctatctg
    13261 aaaacaaaat caacatccag atgatgtctc aaggttctag tgaagtttct attatgtttg
    13321 ttgtcaataa agaccaagag aaagcagcaa ttaaagccct ctacaatgcc ttttttggag
    13381 aaagtaagga agactaagca tggctcaatc acttaacaaa acagtactcc tcagtacgac
    13441 aggcacttcc tacctctcta tcgctgggaa agttgggaaa ttccttgtcg gagatcaggc
    13501 tttggaattt tacccagatg tcaatgtcga acaatttatc cagattcctt ggagccatat
    13561 caatcagatt ggagccaatg tcactggatg caaaatcagc cgccacttcg aagtctttac
    13621 agacaaagga aaattcctct ttgcttcaaa agactcaggt gccattctca aaattgcacg
    13681 cgaaaaacta ggcaatgaca aggtcgtcaa acttccgacc ctgattcaga caattagcca
    13741 aaaatttaaa aatctatttg caaaaaagta gaaactttag tataatcata gcaacggata
    13801 agtaggttat cttcttcttt cagaaagtct gcgggtactg cgagcagata ggagggatag
    13861 gtgaaattct accgttagta acaattacat acacaatcaa gtgcacacct gtgaagttgg
    13921 atggaaccgt ggccctgcca ctccaacgtt ttgtcaggcg tgcttttttc ataaaggagt
    13981 tcttatgtta gatatcaaac gtattcgtac agattttgaa gctgtcgcag aaaaattagc
    14041 tacacgtggt gtagatgctg ctgtcttgaa tgaaatgaaa gaaatcgatg ctaaacgtcg
    14101 taacatcttg gtcaaggttg aaactctcaa agcagaacgt aacacagttt ctgctgagat
    14161 tgcccaagct aagcgcaaca aggaaaatac agatgacaag attgctgcca tgcaaaatct
    14221 atctgctgag gttaaagcct tggatgctga attggcagaa atcgatgcta aattgacaga
    14281 atttacaacg actcttccaa atatcccagc tgacagcgtt cctgttgggg ctgacgaaga
    14341 cgacaatgtg gaagttcgcc gttggggtac tccacgcgag tttgacttcg aacctaaagc
    14401 tcactgggat ctcggtgaag accttggtat ccttgactgg gaacgcggtg gtaaggtaac
    14461 aggcgctcgc ttcctctttt ataaaggcct cggtgctcgt ttggaacgtg ctatctacaa
    14521 ctttatgttg gatgaacatg gaaaagaagg ctatactgaa gtcatcacac cttacatagt
    14581 caaccatgat tctatgtttg gtactggtca gtatccaaaa tttaaggaag atacttttga
    14641 actcagcgat accaactttg tcttgattcc aactgctgaa gttcctctga caaactacta
    14701 ccgtgatgaa atcttagacg gcaaagatct tccaatctac ttcactgcta tgagtccgtc
    14761 attccgttct gaggctggtt ctgccggtcg tgatacgcgt ggcttgatcc gtttgcacca
    14821 attccacaag gttgaaatgg tcaaatttgc caaaccagaa gaatcttacg aagaattgga
    14881 aaaaatgaca gccaacgctg aaaacattct tcaaaaactc aaccttccat accgtgtcgt
    14941 tgctctctct actggagata tgggcttctc agctacgaag acttacgact tggaagtgtg
    15001 gattccagca caaaacaatt accgtgaaat ctcaagctgt tcaaacacag aagatttcca
    15061 agcccgtcgt gcccaaatcc gttaccgtga tgaagcagat ggcaaggtga aactccttca
    15121 taccttgaac ggttctggac ttgcagttgg acgtacagtg gctgcaattc ttgaaaatta
    15181 ccaaaatgaa gatggttctg tgaccatccc agaagcactt cgtccataca tgggtggagc
    15241 tgaagtcatc aaaccataaa aaataaggtt tagctatttc tagctagacc ttttttcgta
    15301 accaaatcag ataagcacct agtacaaaga ataaaatagt taggcatata atggtttcag
    15361 ccaataccag gtaatccaga aatggaagtt tcaaaattcc ctgagccatc ttgagcgagg
    15421 tcgctgtgat aatggttggg aaggtgaggg ctgagaaggc tggttgaaaa ccttgtttta
    15481 aaatgttggg cagacgagtt aaaacaaaga aaaagaagga ttgagaagcc aaaatcatga
    15541 caatcaagac ccaagtcggc aggctggttc ctcctactcg aactagagaa gccaagagta
    15601 gagagaaagg agcacagtag attccttctt gtccaagcaa ggctagtggg agtggatgtt
    15661 tctttaaatc gctataaata aggggataga gatagaaggt caagagaaaa ccaaaactca
    15721 aggtcgcata ggcaatttcg ataataccta ccagaggata ggtcaaggca gccactgcta
    15781 tccccacata gagaaccgtc cagcttggag tggcatgaac cctccgctct ggacaagcaa
    15841 acttgatggt aaaaccagca atcaaggtca aatccaagag aaatgaaaac caccaaatcc
    15901 cttgtgctac caaaggaaga taagagaata cgcgaaagac ataggtcgat aaaatcatcc
    15961 cagccatagg aaaggttgcc attcctgaca aaagaggggg cttggtcaat tcttgcttgg
    16021 tttctttcca attaaagaga tgcagaatta gaaagtaaat ccataaaacc aaaccagtca
    16081 gactaaaaag atgggataga actggcaaca tatctaaaat aagatttcca gctcctgcca
    16141 aacctagcaa acaacctgaa aatactaagg ggagtttttt catcctaacc tccaataatc
    16201 atgttagttt cagtataaca taaaagcgct taaatgagga tttaaaaaaa cgaagtccga
    16261 ttatttcaga cttcatttta ctcagatatg aattaggcat aaggttgcaa ttctggatta
    16321 attggtgtat tagctaagtt gttggcatag ttacagagga ttgctaggct gacaccgaaa
    16381 accacatcca aggcattttg ttgagtgtag ccagcttcta aaaactcaga caaggcttca
    16441 tctcctacac gacccttggt attgataact gccaaggtaa acttagctag ggtatccaat
    16501 ttaggatctg tttcaattgg agtacgattg cgaagagctt gaatcaagtc atcattcatc
    16561 tggatttgtt tgatggaaaa ggctgtgtga cctgcgacac agaaggcaca accattggtc
    16621 acggctgccg tgatttgcac cacttcacgc tcaacgggtg tcaggctgtt gcgacggtgg
    16681 atagatgaga caatttggta ggcttctaaa acagtcgggg cattggccaa gagaccgatt
    16741 aggttgggaa tatagccatt gttgtctttt tctactgttt caagaatttc tttcacttct
    16801 gctggtgctg actctactgt atggatagta aatgttgtca taagatacct cttttcttat
    16861 tattgacact aatattattg gaaaatctta taaaatcctg attcctaagt ttatctaaga
    16921 taaagcttta ttctctcata agattttcgt tgttatatta gtttatcaca cttccaatca
    16981 cttgcataat atatattata tatcaggctg ataaaaatta tttataggca aaaaaatcac
    17041 acgagctgtg tgattccatt atttgtcaaa atacttttta gtttcagcaa taacgactgg
    17101 cgacaagacc aagagggcaa tcaagtttgg cagagccatc aaggcgttaa cgatatctgc
    17161 gataatccag accatatcca actcgataaa tcctcctaac aagaccatga gcacaaaaac
    17221 cacacggtag agccagataa agcgaacccc aaagaggaac tcaaaacagc gttctccgta
    17281 atagttccaa cctagaatcg ttgtaaaggc aaaaagtaca aggaagatgg tcaagagggc
    17341 aggcccaaag tgtgaaaaga ctgttgagaa agctgactga gtcaaggcaa ccccattcaa
    17401 gtcaccactc caaactccag ttaccaagat ggtcaaacca gttagagtac aaatgatgag
    17461 ggtatcaata aaggttcctg tcatggaaat caaaccttgc tctactggtt catttgtctt
    17521 ggcagctgca gctgcaatag gagcagaacc cagaccagat tcgtttgaga acacaccacg
    17581 cgccacacca ttttgaatag ccatccgaac gctagcacca gcaaatccac ctaccgcagc
    17641 aaggggacta aaagctgagg taaagactaa agcgattgtg ccagggattt ttccgatatt
    17701 aaagaaaata actataagag ttcctaagat ataaatgatg gtcataaaag gaacaacagt
    17761 agttgaaacc ttagaaatag acttgagtcc accaaagact gcaatcgcta caaagacaga
    17821 caagacgaga gctgtgatga ctggcgaaat cgtcgttgta ttttggatag attctgtaat
    17881 cgagttgact tgggtgaagg ttccgattcc caagagagca accaatactc ctgctactgc
    17941 aaacaaaaca gcaagtggtc gccacttttc tcccatccct agaaggatat agtgcatggg
    18001 acctcccgct actgcaccat ggtcgtcctt ggtgcggtat ttgatggcca agagtccttc
    18061 cgcatacttg gtagccattc caaagaaagc cgccatccac atccaaaata gagctcctgg
    18121 tccaccaacc ttgatagccg tcgcaactcc tatgatattt cctgttccaa cagttgatgc
    18181 caaggctgta cacagagctg caaaactgga tacatcacca tgtcccttat cctggataaa
    18241 aataagctga aaggccttgg gcagacgcaa aacctgcaag agtcctagcc gcatggttag
    18301 gtaaatccct gttccgacca ataaaatcaa gaggggcggt ccccaagcaa aatcatcgat
    18361 tgatttaagc aattctaaca tttccttctc ctatcgtttc aaccccaaaa gaaagagcac
    18421 atgcaagata catgtactct ggaatgctta gataaatgct aaaaagcggt ctatcctagc
    18481 tctgtccttt tacctgagag tttgagcagt tgcctgcctt gccccttcgg tgcctttacg
    18541 gtctctccag agttccgtcc atttacagtc atggaaaatc aaacgattcc ccacttctat
    18601 taaacttcat tcggtgttgg tatttaattg attctaattt tacaaaaaat gttggctttt
    18661 gtcaatgtgt ttattagtaa aaattagttc aacagttttt actttataaa gtccagaata
    18721 ctgctatcct ttaaaagtga caatagtcgc accactgcct ccagcatttt gtggggcata
    18781 gccgaaactc ttgacatgtt tgtttctttg caagtatttg gtaactcctt cacggatgac
    18841 tcctgttccg ataccatgga tgatatcaac ttgagccata ttgttaagca aggcttggtc
    18901 gatgaaggta tctagctcat tcatggcttc ttcatagcgc ttgcctcgaa gatccagtct
    18961 agcttgaggt cctcgcccag aagttcgttt cacaacattg acctgtttct tcttgactgg
    19021 tttttcttgc tgggcttgaa caagatcaaa ctctttctct tccaaggtca tcttaatcaa
    19081 gccaacttgg gcttcccagc ggccgtcctt gagttgactg gtcaaggtac cacgctgacc
    19141 ataactgaga accacgatat catctcccac ctttggagct cgttttttct tggccttttg
    19201 aaggaccttg tttttagaca agtccacttt ttcaggagcc aattttttca acttggcctt
    19261 ggcttcaatg atttcgtggg gcttgagttg ggatttactg tggagatttt tgagaatctg
    19321 gtcactttca cttagggcca tatccacaat ctcagcagcc tgttcacgcg ccttgttaag
    19381 ctcggtttcc ttttcacgat taagctcgtt gtagagtttt tttagcgcac ggttcatctt
    19441 gagattttct tgctccacct cacggatatt gtccaaacgt ttgcggcttt ccagcgtctg
    19501 ctcttctaat tgctcaatga tacgattgac gtcattgtcc tgatcgatct gctgactggc
    19561 atctcctacg ataacttcag ataggcctag acgtttggca atttcaaagg cattacttcg
    19621 gccaggaaca ccctgcataa agcgataggt cgggcgaaga gttgcagtat caaactccat
    19681 actggcattt tgcacaaagg ctgtctcaat accgtaggcc ttgagttctg gatagtgggt
    19741 cgtcgccatg gtcttgattt gacgcaggcg aaggtcctcc agaatagcca tggcaagggc
    19801 tgctccctct tggggatcag taccagcccc caactcatcc aaaagtaaga gtgaatgttg
    19861 gttgaccttg ccaagaatat ccacgatatt ggtcatatgg ctagagaagg tagacaagct
    19921 ctgctcaata gactgctcat ctccaatatc agcaaagatt tcttcaaaaa taccaacacg
    19981 acttcccttg tctgctaaaa tcggcaatcc tgactgggcc atgacctgtg tcaagcccag
    20041 agttttgagc atgatggtct tcccacctgt attgggacct gtaatgacaa tagctgttaa
    20101 atcttgacca aaatagacat catttgcgac ggcatttttg accaaaggat ggcagacatg
    20161 gagcagttga atctcttgat tttctgacag ctgaggcacg actgcttgtc tttcttggat
    20221 aaatcgaacc ttggcacgaa tcaagtccag atgaccgata atccaagcgt cattagcaat
    20281 ctcagccgca tgagggcgga cacgctcaga aatttcttgg agaatgcgaa gcatttcata
    20341 gcgctcatct gctcgcagac tagcaatttc ttcgctcagt ttgactacct cacgtggttc
    20401 gatatagacg gtgtttccac tagcagaaat atcatgaacg acacctgcaa tcttattgcg
    20461 gtaggtgttt ttgactggta aaacctgacg gccatttctg ctagcaacaa ttccttccgt
    20521 caacatctgc gctttttgct tgagtaagtc ttgtaaaaca tcgcgcacct gactctcgct
    20581 atcatgtatt tttcgacgga ttcgcgccaa ttcttcactg gcaaaatttt caatgaaacc
    20641 cgcatcatta aaggcctgaa gatttccttg taattgcgga aaatcatgta atttctcaaa
    20701 ccaaagggct aattcttcca agctgacatt ttccagattg gcgtaaaaat tttgaagttc
    20761 tcggctggca agaagcacgc gtttcaagag taggaactcc tcgatattga gatccgctcc
    20821 catctccaac ctcttgcaga ctcctgcaat ttccttagtt gagagaatag taaaatgcgg
    20881 ttgctcgacg aaaagagcct gcatttcttt catctcagca aaagcctgtt tgattttatc
    20941 tgctttggca gtcggagcca gctgtctcaa ttgctccaag ccctgctcgg tcaacaaatg
    21001 aggctcaaac aaggccttga ccttatcgaa ctctaatgtt tctaatattt tcttattcat
    21061 ctttatttcc ttgtctttga atgtctaggt gtggtagctt tttacattct gatagattcg
    21121 agtcaaatct gtaaacaaag ggctaggaaa actcctgccc tttttatccg attaaatttg
    21181 tcacccagat ttgtttgagc caactggttg ttatcggtat gctctggatg atgtgttttg
    21241 cgacgatact cttttcaaga ggattttgta taactgccat ggggatggtc gccaagattg
    21301 tcaaggccat ttgcaagaca aataaggtca ccaacatgga caagatacct gctgaaactt
    21361 ggaacaactt accacccagt tttttgctag gaatcaagtg taagagaaga ccaagcaaac
    21421 gaccaatgct atagacaatc ccaaatacaa gcaagtagcc gatacctgca taaaagacct
    21481 tatccagctg aaagagttga tccgatggga aaaagaaagt cccctgacct tcctgcgaat
    21541 ttgcataagg gagcaataaa tggaattgct ctccaagccc cttataaaac tggccagcca
    21601 taaaagccga tgccatggct gaaatcaggt aataaacctg taagagcagg cctctccgat
    21661 agccgatata aaatccccaa accaagacca atagaagaag gagtgaaatc ataaggaatc
    21721 ctcaatcttg ctctgttctt gcttacaagt cacaagcttg tgacggagtt cttctagctc
    21781 ttgctcctta tcgtcaaatt caatctcacg gctgagctga gttgataaac agttgactgc
    21841 caacaaaaga gcgattgttt catcatctgt gctaggcatt tgttctttaa ttgcttggta
    21901 tttttctgtc gcaaccttag cgatttcctc cataaaaagg ttatcatgtt cgcttgtcaa
    21961 ggttaacgat tttttcccga atgtaaattt gaatcgattt agatttgcca taaaattcac
    22021 ctcacgatat tataccaaaa ttcgctaatt ttgtcagttt ttacaaattt tactgctttt
    22081 gtggtacaat agaaactatg gcaagtatca cactcacacc aagcgaaaag gatattcagg
    22141 cttttcttga acactatcaa accagtctgg ctcctagcaa gaatccctat atccgctact
    22201 ttttgaaact acctcaagca acggtttcta tctatacttc tggaaaaatc ttgcttcagg
    22261 gtgaaggggc tgaaaaatac gccagtttct ttggctatca agctgtagag caaaccagcg
    22321 gacaaaatct tcctttgatt gggacagatg aggtagggaa tggttcctac tttggtgggc
    22381 ttgcagttgt ggcttccttt gtcacacctg accagcatgc cttcttacga aaactcggtg
    22441 tgggagattc taagactctg accgaccaaa agattcgtca gattgctcct attctcaagg
    22501 aaaaaattca gcaccaggca ctccttctct cacccagcaa gtacaacgag gtcatcggag
    22561 accgctacaa tgctgtttcg gttaaggttg ccctccataa tcaggctatc tatctcctcc
    22621 ttcaaaaagg tgttcagcct gagaaaattg tgattgatgc ctttaccagt gctaaaaatt
    22681 atgacaagta cttggcacaa gagaccaatc gtttcagcaa tcctatcagc ttagaagaaa
    22741 aggctgaggg caaatacttg gctgtcgcag tttcttctgt cattgcgcgt gatctctttc
    22801 tggaaaatct tgaaaatttg ggacgagaac tgggttatca gctcccaagt ggagctggaa
    22861 cggcttctga caaggtggct agccagattt tgcaagccta tggtatgcag ggactcagct
    22921 tctgcgccaa attgcacttt aaaaacactg aaaaagcgaa aaaacgctta gaaaggtaag
    22981 ttatgaattc atttaaaaat ttcttaaaag agtggggatt gttcctccta attctgtcat
    23041 tactagcttt aagtcgtatc tttttttgga gcaatgttcg cgtagaagga cattccatgg
    23101 atccgaccct agcggatggc gaaattctct tcgttgtaaa acaccttcct attgaccgtt
    23161 ttgatatcgt ggttgcccat gaggaagatg gcaataagga catcgtcaag cgcgtgattg
    23221 gaatgcctgg cgacaccatt cgttacgaaa atgataaact ttacatcaat gacaaagaaa
    23281 cggacgagcc ttatctagca gactatatca aacgcttcaa ggatgacaaa ctccaaagca
    23341 cttactcagg caagggcttt gaaggaaata aaggaacttt ctttagaagt atcgctcaaa
    23401 aagcccaagc cttcacagtt gatgtcaact acaacaccaa ctttagcttt actgttccag
    23461 aaggagaata ccttctcctc ggagatgacc gcttggtttc gagcgacagc cgccacgtag
    23521 gtaccttcaa agcaaaagat atcacagggg aagctaaatt ccgcttctgg ccaatcaccc
    23581 gtatcggaac attttaagag acctaagagg ccgagaatca ccaatctcag cctcttcttc
    23641 tatcgtgaga agtgattgtt tattgacaaa tactcaatga aaatcaaaga acaaactagg
    23701 aagctagccg taggctgctt aaaacagtgt tttgaggttg tggatagaac tgacgaagtc
    23761 agctcaaaat actgttttga ggttgcagat ggaagctgac gtggtttgaa gatattttcg
    23821 aagagtataa aataaaaaca atcccaactc tcacctattc atgcaaagga attttatgga
    23881 agtttatttt tcaggaacca ttgaacggat tatttttgaa aatcccagca atttttatcg
    23941 catcctcctc ctagaaatcg acgatacgga cgcagaggat tttgatgact ttgaaatcat
    24001 tgtcacagga accatggctg atgtaattga gggcgaagac tatacttttt gggggcaaat
    24061 tgtccagcac tccaagtatg gagaacaact gcaaatcagt cgttatgatc gcgcaaaacc
    24121 aactagtaag ggcttggtca agtacttttc aagtagccat ttcaagggga ttggtctcaa
    24181 gacagctcag aaaatcgtgg atacctatgg cgaaaatacc attgacgaaa ttttgcaaca
    24241 cccagaaaag ttagaaggca tcgcaggact ctctgccaaa aatcgcgagg ctttcgtctc
    24301 cactctccgt ctcaactacg gaacggagat ggttttggcc aaactagcca actacggcat
    24361 tcccaacaaa ctagcctttc agattcaaga cttttacaag gaagaaaccc ttgatgtggt
    24421 tgaaaattat ccctaccagc tggttgagga tatcaagggt ttgggcttta ccattgctga
    24481 ccaactagct gaggaactag gcatcgaaag tcaggctcct gaacgcttcc gcgccggtct
    24541 agttcacagt ctttttcagg cctgtatgga aacaggggac acctatgttg aagcacggga
    24601 tttgctggaa caaaccctta ctctccttga gtcttcccgt cccgtagaac tagaccccag
    24661 ccaagttgcc caagagctct cctacctgat cgaagaagac aaggttcagc agattgatac
    24721 caagatcttt gacaacagcc tctttttcgc tgaggaaggc atccgcagtc acttggttcg
    24781 tatccttgaa aaaggaaaac agaagagtca tgatttagaa accattcaaa aacatatcac
    24841 tactatcgag gaagagctgg gaattgagta tgataacatt caaaaacagg ctatctgtga
    24901 tgctatccag aataaggtct ttatcctgac aggtggacct ggtactggta agacaactgt
    24961 tatcaatggg atcatcgctg tttacgccct gttagaggga cttgacctca ggaagaaaag
    25021 caatctgccc attcttcttg ctgctccaac tggacgagcc gctcgccgca tgaatgaatt
    25081 gacaggtttg cctagcgcga ccatacaccg ccacttggga atgacaggtg acgatgatac
    25141 cagtcatctg gaagattacc tagatgctga ctttatcatc gtggatgaat tctctatggt
    25201 ggatacttgg ctggccaatc aactcttctc caatatctct tctaacagta agatcctcat
    25261 cgtaggtgac agtgatcagc taccttctgt cagtcctgga caggttctag cggatctgct
    25321 tcatattcct ttgattcctc agactcgctt ggaaaaaatt tatcgacaaa gcgaagaatc
    25381 aaccatcgtc accctagcta gtcagattcg acagggcatc ttgccagctg atttcaccca
    25441 aaaaaaagct gaccgttcct actttgaaat tgctagtggc catattcctg ccaccattga
    25501 aaaaatctta ggtgccgccc tcagaaatgg tattcctgcc cgtgatatcc aagttctggc
    25561 tcctatgtac cgagggacgg cagggattga tgctatcaat cagctcatgc aagacctgct
    25621 caatccccca caaaaagatc aactcagttt tgaagttcct cagtgccact atcgtaaggg
    25681 agacaaggtc attcatttgg ttaacgatgc tgaaatcaat gtctttaatg gggatttagg
    25741 agctatcaca gacctgattc ctggtaaata caccgaatcg aaacaagacg agattgtcat
    25801 tgattttgat ggtaatgaag tctcttaccc ccgtaacgaa tggtacaaga ttcgcttggc
    25861 ctatgccatg agtattcata agtctcaggg aagtgagttc ccagttgtca tcctaccgat
    25921 tactagtgct agccgacgta tgctggagcg aaatctcatc tacacagcca ttacacgtgc
    25981 caaaagcaaa ctcatcttac taggcgaatt acaagccttc gactatgcta cccaacacat
    26041 cggaactgcc cgaaaaacct atctgattga acgcttcagt gatctactgg agaatgttga
    26101 agaaaagcaa caagctgtct ctgaaacagt cacatcaagt gcctctgaac aatcctacat
    26161 cctaaccgaa gaaaactggg acagcattcc agccatgatt gggattacag acacagacct
    26221 caaagagatt tttggaaaat agtgcatcag aaaaaccacc aattcaggtg ggttttctgt
    26281 ttattaagat tattttactg ttgctgtgct tgtaatcaat tcaacagctt ttttgatagt
    26341 gatatcatgt ttcaacatgt cagctgaaag caagttttga acttgtgcaa cttccatgtt
    26401 gtagtctgct gccaattgct caacttcttt ttggatttct tcttctgaag catcaaatcc
    26461 ttcagctttg gcaactgctt cgataacaag gttagtctta gtacgtgact cagcttctgc
    26521 ttggtattgg ttgtgaaggt cttcttgagt agttccagtg atttggaagt acatgtcagg
    26581 gttgatccct tgacgttgca agttcccaag gaattcattt actgaacggt gaacttcttc
    26641 gtggatcatt tcttctggaa gttctacgat ttcagcattt tctacagctg tatcaattgc
    26701 tgcaccttca actgcatctt tgtaagcttc ttctttagca gcagccaatt ctttgcggta
    26761 tttttctttc aagtcagcaa gtgtttcaac ttcttcatca atgtcttttg caagttcatc
    26821 gtcaagagct ggaacttctt tagcttttac ttcgtggata gttgtcacga atttagcttc
    26881 tttacctgca aggtcttctg cttggtagtc ttctgggaat gttacgataa catcaacggt
    26941 ttcgccagct gagtgaccta ccaattggtc ttcgaaacca gggatgaatt gacctgaacc
    27001 aagtccaagt gagaagtttt caccttttcc accgtcaaat tcaacaccgt cgatagaacc
    27061 aacgaagtcg atcacaacag tgtcgccgtt ttcagcagca gcttccttga taaccaattc
    27121 agccaggttg ttgcgttcgc gttcgatacg ctcttcaaca tcagcgtcag ttacttcttt
    27181 ttctacatca actgatactt caaggttttt gtagtcaccc aattttactt caggttttgt
    27241 aacgacttca gcagcgataa cccagtcttg acctttttcc attgaagtta cgtcaatttt
    27301 tggttgggca accacttcaa gaccagcttc ttttacagct gcttcataag cgtttggcaa
    27361 aagagcgttc ataacgtctt ggtaaagtga ttcttcacca aattttttat cgaagatagg
    27421 acgtggaagg tgacctttac ggaaacctgg aacattaaga gatttcttca ctgacttgaa
    27481 gacacggtcc aattctggtt tgatttggtc ttgagagata gtgaaagtca agacaccacg
    27541 gtttgtttct ttgttttcaa atgatacaga cattctgtca tttctcctta aaatttttta
    27601 aatacagtct attataacat tttctataaa taaaatctag actaatgcag ccaattatag
    27661 acaacaagcc tttttcctaa ctaa
//
LOCUS       DAVKHZ010000019        27502 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000019 DAVKHZ010000000
VERSION     DAVKHZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.019"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            136..2316
                     /locus_tag="VWW99_000769"
     CDS             136..2316
                     /locus_tag="VWW99_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9538645.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEASALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VWW99_000770"
     CDS             2368..3183
                     /locus_tag="VWW99_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9538646.1"
                     /translation="MKFLTLNTHSWMEKEAKEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVVLAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            3323..4666
                     /locus_tag="VWW99_000771"
     CDS             3323..4666
                     /locus_tag="VWW99_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9538647.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VWW99_000772"
     CDS             5043..6233
                     /locus_tag="VWW99_000772"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9538648.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6786..7721
                     /locus_tag="VWW99_000773"
     CDS             6786..7721
                     /locus_tag="VWW99_000773"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9538649.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7755..8792
                     /locus_tag="VWW99_000774"
     CDS             7755..8792
                     /locus_tag="VWW99_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9538650.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8965..9570
                     /locus_tag="VWW99_000775"
     CDS             8965..9570
                     /locus_tag="VWW99_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9538651.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9727..10257
                     /locus_tag="VWW99_000776"
     CDS             9727..10257
                     /locus_tag="VWW99_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9538652.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10278..11363
                     /gene="rlmN"
                     /locus_tag="VWW99_000777"
     CDS             10278..11363
                     /gene="rlmN"
                     /locus_tag="VWW99_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9538653.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVNHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQINLAVS
                     LHALNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLRFNTMKRDRQKAVAAVNP"
     gene            11363..11845
                     /locus_tag="VWW99_000778"
     CDS             11363..11845
                     /locus_tag="VWW99_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9538654.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            11847..13388
                     /locus_tag="VWW99_000779"
     CDS             11847..13388
                     /locus_tag="VWW99_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9538655.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(13444..14247)
                     /locus_tag="VWW99_000780"
     CDS             complement(13444..14247)
                     /locus_tag="VWW99_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9538656.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(14331..14552)
                     /locus_tag="VWW99_000781"
     CDS             complement(14331..14552)
                     /locus_tag="VWW99_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538657.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEKVEDFQDDDEIPTII"
     gene            complement(15031..15231)
                     /locus_tag="VWW99_000782"
     CDS             complement(15031..15231)
                     /locus_tag="VWW99_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538658.1"
                     /translation="MNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMNLLAD
                     DSLAGDIILLWRIQFGTFTTET"
     gene            15410..15682
                     /gene="rpsP"
                     /locus_tag="VWW99_000783"
     CDS             15410..15682
                     /gene="rpsP"
                     /locus_tag="VWW99_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9538659.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            15702..15941
                     /locus_tag="VWW99_000784"
     CDS             15702..15941
                     /locus_tag="VWW99_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9538660.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            16518..17306
                     /locus_tag="VWW99_000785"
     CDS             16518..17306
                     /locus_tag="VWW99_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9538661.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            17337..17855
                     /gene="rimM"
                     /locus_tag="VWW99_000786"
     CDS             17337..17855
                     /gene="rimM"
                     /locus_tag="VWW99_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9538662.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            17845..18564
                     /gene="trmD"
                     /locus_tag="VWW99_000787"
     CDS             17845..18564
                     /gene="trmD"
                     /locus_tag="VWW99_000787"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9538663.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            18576..18914
                     /locus_tag="VWW99_000788"
     CDS             18576..18914
                     /locus_tag="VWW99_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538664.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(18944..19264)
                     /locus_tag="VWW99_000789"
     CDS             complement(18944..19264)
                     /locus_tag="VWW99_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538665.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            19362..19577
                     /locus_tag="VWW99_000790"
     CDS             19362..19577
                     /locus_tag="VWW99_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9538666.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(19646..19732)
                     /locus_tag="VWW99_000791"
                     /pseudo
     CDS             complement(19646..19732)
                     /locus_tag="VWW99_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(19783..20319)
                     /locus_tag="VWW99_000792"
     CDS             complement(19783..20319)
                     /locus_tag="VWW99_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9538667.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(20516..21862)
                     /gene="gor"
                     /locus_tag="VWW99_000793"
     CDS             complement(20516..21862)
                     /gene="gor"
                     /locus_tag="VWW99_000793"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9538668.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            22148..23347
                     /locus_tag="VWW99_000794"
     CDS             22148..23347
                     /locus_tag="VWW99_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9538669.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKISEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KTDEATN"
     gene            23331..24032
                     /locus_tag="VWW99_000795"
     CDS             23331..24032
                     /locus_tag="VWW99_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538670.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            24034..25293
                     /locus_tag="VWW99_000796"
     CDS             24034..25293
                     /locus_tag="VWW99_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538671.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            25417..27414
                     /gene="metG"
                     /locus_tag="VWW99_000797"
     CDS             25417..27414
                     /gene="metG"
                     /locus_tag="VWW99_000797"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9538672.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVKRYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
BASE COUNT         8400 a         5041 c         5987 g         8074 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc ttcagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 gctagtcaat accaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgacgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtcatg aaagggcaag gggttcaagc tatctacggt
     1741 ccaaaagctg acatattgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaagat
     1861 cttactgagg aagtttattc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc cgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgta tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcaaagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccatgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gtcctagctg aaactgtagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatatt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attctagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcggat atgaccttgg atatgggatt cttggaaact gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat ccaatcctgt tatggagaaa attaagacca aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccagttg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggcgatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttggcagc acgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagacgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa accaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgtcaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgtgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt aacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt atagtagatt gaaactagaa tagtacactt
     6361 caacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttc tcctgttctt
     6421 gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc tcgtaataga
     6481 taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta ttgcaaggtt
     6541 ttctagagca acagagtcat ggaatggact catggttgag atttctcctt gttgcttgga
     6601 cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact agctgtttct
     6661 atagcatgac ttctgtacta gactttcttt tccaaataaa tagatagaac cacagaatct
     6721 agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc tggaggatta
     6781 gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat tgcttgatga
     6841 atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa aactcagcgg
     6901 caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat cctgagccac
     6961 gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat aatggcttag
     7021 actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga actttcttct
     7081 tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa gtccaagaga
     7141 gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca ggtaaacctc
     7201 agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt gcttacttca
     7261 ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt gatcaagcgg
     7321 cagctatttt caacaaatat ccgctcaagt ttgtcaactg cgttaactct atcggaaacg
     7381 gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt ggtggaattg
     7441 gtggagaata cattaaaccg actgctctag ccaatgttca cgccttttat caacgtttaa
     7501 atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat gcctttgaac
     7561 acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa gaaggcgtca
     7621 gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa ggctacgaga
     7681 gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatca aaaaatctga
     7741 agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta tcaaatttac
     7801 cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg aagagtcaaa
     7861 tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt gatatgaaag
     7921 aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt gcggatgaga
     7981 aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc tttttgacag
     8041 atgatgagct taagtcattt gaggaatacc ttgacaatcc ttctccaaca accaagttga
     8101 taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta cttaagcgtg
     8161 atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag tatttccaaa
     8221 agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat ctcctcatca
     8281 agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag tcctataagg
     8341 cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc ttgcaggaca
     8401 atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg cgcgatttgg
     8461 tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc atgctgggac
     8521 aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca gaatcgcaga
     8581 ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa atcaagtttg
     8641 cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt tcctatttga
     8701 ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt tttgaaaagg
     8761 cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa cccgcgtcag
     8821 gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt atgtcagtga
     8881 tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat cattttcata
     8941 tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc atatgcatac
     9001 gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga caaacaccat
     9061 caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat cggtgaagac
     9121 cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca agcacttatc
     9181 aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac tcccgagaaa
     9241 acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc atttgaagaa
     9301 ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg ggcatggttg
     9361 gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga cacaccaatc
     9421 tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta ctacgtgaaa
     9481 taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa ctggaataaa
     9541 gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat tgttcttctc
     9601 ttttttaggt tataagattc tggtctgaca aaatcgtcag acttttttca tttttatgag
     9661 aaacgtgcta actgctagaa attatggtag aataaagtat taagtaatcg tggaaaaagg
     9721 atatctatgc gaaaagaaat tgcacctgaa ttatacaact ataacaagtt tcctggtccg
     9781 gagttccatt tacacgggga caaggtcgaa acggaaggga tagctttttc cttggttgaa
     9841 aatatcaagg atgcctttga tgtgacgact tttaatcagc gtttttcaga agtattaacc
     9901 aagtttgatt atatcgtggg ggactggagc aacgaacagc ttcgcctacg aggtttttac
     9961 aaggatgacc gaacagaaga aaaacttgaa aaaatcagtc gtttacaaga ctacctttta
    10021 gagtattgta gttatggttg tgcctatttt gtcttagaaa atgaagcccc taagcgagca
    10081 tcatttgaca agaaaatgcg taagacggaa gaagaaacac cttctagaaa aggaaagaaa
    10141 ccgactcaaa ctaaacgaaa gtcgaatgca gataagaaaa atagacgtcg tcagaaagac
    10201 cagcattctc agaaagagga caaggaacaa cgtcattttg tcattcgtca gaagtgagta
    10261 gagaataagg agaagaaatg aaaccctcaa ttcatagttt ggcacatcaa accatgcagg
    10321 aatgggtatt ggagcaggga gaaaagaaat tccgtgcaga tcaaatctgg gaatggctct
    10381 accgtaaacg tgtgcagtca tttgaagaaa tgaccaacct ttccaaggat ttgattgcta
    10441 agctcaatga ccagtttgtg gtcaatccct tgaaacaacg tattgtgcaa gagtctgctg
    10501 atggtacagt caaatatctc tttgaattgc ctgacggtat gttaattgag actgtactca
    10561 tgtgtcaaca ctatggttta tctgtctgtg tgaccactca ggtcggctgt aatatcggtt
    10621 gtaccttctg tgcctctggt ttgattaaga agcagcgtga cctcaataac ggtgaaatcg
    10681 tagcgcaaat catgctggtt cagaaatact ttgatgagcg tggtcaggat gaacgcgtca
    10741 accatatcgt tgtcatggga atcggtgagc cctttgataa ttacaacaat gtcttgaatt
    10801 tctttcgtac tatcaatgat gataaaggaa tggctatcgg tgctcgtcac atcacggttt
    10861 caacctcagg tttggctcat aaaattcgtg actttgctga tgaaggggtt cagatcaatc
    10921 ttgccgtgtc tcttcacgca ctcaacaatg aacttcgttc aagtatcatg aagattaacc
    10981 gtgcctttcc gattgaaaaa ctctttgctg ctattgaata ctacattgag acaacaaacc
    11041 gtcgtgtaac ctttgaatat atcatgctta atgaagtcaa tgacggtgta gaacaagcct
    11101 tggaattggc tgaattgctc aagaacatca agaaattgtc ttatgtaaac ttgattcctt
    11161 ataacccagt tagtgagcat gaccaatata gccgtagtcc caaagagcgc gtgttggctt
    11221 tctatgatac gcttaagaaa aaagggggca actgtgtggt tcgtcaagag catggtacag
    11281 atattgacgc agcttgtgga caattgcgct ttaacacaat gaaacgtgac cgccagaagg
    11341 cagtcgcagc ggtaaatcct taatgatgaa gaagacttat aatcatattt tggtctgggg
    11401 agtcattttc tatagcattt gcattgtctg tttttgcttt actcctcaag aacaatctac
    11461 cgtgggagtg ggaactccag gtattcagca tcttggacgc ctggtttttc ttttgactcc
    11521 tttcaattct ctctggaaac tgggcgaagt gagtgacatt ggacaattat gttggatttt
    11581 tttacaaaat atcctcaatg tcttcttgtt ttttcctctg attttccaac tcctttatct
    11641 atttccaaat ttgcggaaaa caaaaaaggt ccttcttttt agttttcttg tgagtcttgg
    11701 aatcgagtgt acgcaattaa tcttagactt tttctttgat ttcaatcgcg tctttgagat
    11761 tgatgatttg tggaccaaca ctttgggtgg ctatctggct tggctccttt ataaacgatt
    11821 acataaaaac aagataagga attaaaatga gtattttaga agttaaaaat ctgagtcacg
    11881 gttttggtga ccgtgcaatt tttgaagatg tgtccttccg tctcctcaag ggagaacata
    11941 tcggcctggt cggtgccaat ggtgaaggaa aatcaacctt tatgagtatc gtgactggta
    12001 aaatgctgcc agatgaagga aaggttgagt ggtccaaata tgtgacggct ggttacttgg
    12061 atcagcactc tgtccttgct gaaagacagt cggtgcgtga tgttctccgt acggcttttg
    12121 atgagctttt caaagctgaa gctcgtatca atgacctcta tatgaaaatg gctgaagacg
    12181 gcgcggatgt tgatgctctc atggaagaag taggagaact tcaagaccgt ctggagagtc
    12241 gtgatttcta taccttggat gctaagattg acgaagtagc gcgtgctctt ggtgttatgg
    12301 actttggcat ggatacggat gtaacttctt tgtcaggtgg gcaaagaacc aaggtgcttt
    12361 tggcaaaact tctccttgaa aagcctgata ttttgctgtt ggacgagccg accaactact
    12421 tggatgctga gcatattgat tggctcaagc gctatctcca aaactatgag aatgcctttg
    12481 ttctcatttc gcacgatatt ccattcctca atgacgttat caatattgtc tatcatgtgg
    12541 aaaatcaaca gctgacgcgt tactctggtg actactacca gttccaagaa gtttatgcta
    12601 tgaagaaatc tcagctagag gcagcctacg aacgccagca gaaagagatt gcagacctca
    12661 aggactttgt ggctcgtaat aaagcccgtg ttgcaactcg taatatggct atgtctcgtc
    12721 aaaagaaatt ggataagatg gatattatcg aactccaaag tgagaaacca aaaccatcct
    12781 ttgatttcaa accagctcgt acaccagggc gctttatctt ccaagccaag aacttgcaaa
    12841 ttggttacga ccgtcctctt actaagcctt taaatcttac cttcgaacgc aatcaaaagg
    12901 ttgcgattat tggtgctaat ggtattggaa aaacaactct cttgaagagt ctcttgggca
    12961 ttatctcgcc aatcgctggg gaagtggagc gtggagatta tttagaactt ggttattttg
    13021 agcaggaagt agaaggcggt aatcgccaaa ctcctcttga agctgtctgg aatgcctttc
    13081 ctgcccttaa tcaagcagaa gtccgtgcag cccttgcccg ttgtggtttg acaaccaaac
    13141 atattgaaag ccagattcaa gtattatcag ggggagagca agccaaggtt cgtttctgtc
    13201 tcttgatgaa tcgtgaaaac aacgttttag tgctggacga gccgaccaac catttggatg
    13261 tggatgcaaa ggatgagctc aaacgcgctc tcaaagaata taggggatct atccttatgg
    13321 tctgccacga gccagacttt tatgaaggct ggatagacca aatatgggat tttaataatt
    13381 taacttaaga atgagagtaa aagaaataca gtaccgaatg tggatactgt atttttttgg
    13441 tttttaagat ttaaaatcgt agtctttcac cacttcgatg ctgtcgattg tgatagcagt
    13501 agttggcttg tctttttcat ctttttcggc cttagcaatc ttatccacaa catccatacc
    13561 gtcaatcact tgaccaaaga ctgggtgttt gccatctaga ctagggtttc caccttcttt
    13621 gtaggcttcg ataattttct gtggatactt gcttgtaggg agtttagaag aggtatctgt
    13681 agagttttgg ttgatgaaga actggctgcc attggtgttt ggttgaccag tattagccat
    13741 agcaagagca ccgcggatgt tatacaaata aggagtaatc tcgttcttga aaccagttcc
    13801 tttgtcttta gtcttatcct tgtcatgcca gatggactga ccacctgtac cgtccccttt
    13861 tggatctcca gtttggacca taaagccatc gatgacacgg tggaaggtaa taccgttata
    13921 gtagccttct ttggcgtgag tgaggaaatt ttcaaccgct agaggagcga gtttagggaa
    13981 gagtttaatg cgaatatcac cttggcttgt gtggagaatc acttcggctt catcttcagc
    14041 aacttcctta gatagttgtg ggaaattggc gttttcgttt gttaaagcat cgtttaactc
    14101 cttggcagat tgggcagcta ctttggaact ttcttcagca gcaagactgg aatcaacata
    14161 atcatcacca cgcagactgc gttggacgct gctacaccca gctagggcta cagtagacag
    14221 taaaagaagg gttgctagtt ttttcattct gatcctctca aaaattcatt tataaccatt
    14281 gtaccctaaa aggaagtgga tttcaaatat tagtagttta cagaactggc ttagataata
    14341 gttgggattt catcatcatc ttgaaaatct tcaacttttt caacttcttc cccagtaaat
    14401 ggtttctgaa aagaacttag tataaaacgg gcaattggtt caacaattag gaaatttaga
    14461 ggaagagctg caataaagtt tacgaaccag gcgcttagat aggtattggg tgtgatagca
    14521 atgccattgt aaaaaagtcc atagaaggac atgaaagtga ccagctaatt cttcttctga
    14581 aaaatgctca tgaagggctt gatccaggtc gcaagatttc attttagaaa gtccagtaac
    14641 tcccttcttc ttcaggatat tctttttcat agtagtattt agatggtcaa gtgagtcaaa
    14701 agaagtttcg acaagagcat accctttttc taccagagtt tttagatgtt taggggcatc
    14761 aatgccataa gtgtactcaa agtatttagg gaaccagatc ttaagaaagc tcgtaaagct
    14821 agccagttct ctaaacgtta agaactagct acaatacagc atttacaaca cttcatggtt
    14881 cacaccatgg ggtgtttttt aatgatttta agcaaaattc acacttaatt gaaagttatt
    14941 taaattagat gaaaatttat tttttgaaaa agtgggaaaa cattgattct aagcgaattt
    15001 tgaatcatat tcgaatggga gtttttaaaa tcatgtttca gtggtaaaag taccaaactg
    15061 gattctccat agtaaaatga tatctcctgc tagtgaatca tctgctaata agttcatatt
    15121 tcgcttggga acgggcttgg gacttaggag ccaagtatcc aaatctcgtt caggcgatat
    15181 gaagggtttg acttggtgtt ctttataaaa tttttctaaa atagtattca cggatagtat
    15241 ctttcaaaca tttatttcct attctaacaa atttctaaat actttaagaa atttatagtc
    15301 tgtcaagttt ttttcttgac acatgccaga aatctgctat aatagaacat gtgctaaata
    15361 gctcagctat ttcaccgaaa taaaaaatta aagaaatgag actttaaaaa tggcagttaa
    15421 aatccgtttg actcgtatgg gttctaagaa aaaacctttc taccgtatca acgtagcaga
    15481 ttcacgttca ccacgtgacg gacgtttcat cgaaacagtt ggaacttaca acccacttgt
    15541 tgctgaaaac caagtaactt tgaaagaaga ccgcgttctt gcatggttgg ctaatggagc
    15601 tcaaccttca gacacagtac gtaacatcct ttcaaaagaa ggcgtattga aaaaattcca
    15661 cgattctaaa ttctcaaaat aagtttaaag taggttgaca gatggatacg attgaaaatc
    15721 tcattattgc gattgtgaaa cccttaattt cacaaccaga tgccttaact atcaagattg
    15781 aagatacacc agaatttttg gaatatcatt tgaatcttga tcaaagcgat gtgggtcgtg
    15841 taatcggtcg taagggtcgc actatttctg cgataagaac gattgtctac tctgtcccaa
    15901 ctgaatacaa aaaagtaaga atcgttattg acgaaaaata agaagggcgg gacggatgtc
    15961 tcgctttttt gtgtggaata gaagatatag taaaatgaaa caagaacagg acaaatcgat
    16021 caggacagtc aaatcgattt ctaacaatgt tttagaagta aaggtgtact attctagttt
    16081 caatatacta taaaagattg aacgtttagg caattccaag actacttact cgaacattaa
    16141 cagcagtctc ggactgatga agttttcttt atgaagttag tggaggaagt cggagaagta
    16201 gccgaggttt tgaataggca ctctggtcga aaagaaggga taggattcga acgaggaact
    16261 tgccagagaa ctggctgata tcatttacta caccgtcgca atcgtagcta ttaatcatat
    16321 tgaccttacc aagactgtct ttgagaaaga caaaaccgca gccgttaatt accaacataa
    16381 acataatttg gaaggattat tgaagggaaa gtagagatat tgtccccttc ctccaacttg
    16441 agaaaatcct tggaacgtga taaaataaag gataaggatt tttaataatt cattatatag
    16501 aatcagtgga tttctttatg aagaggtttg aagtatctac agaaattggt agcctctctg
    16561 ttacttatca aaagcaaaag aaagtgctag tttgtctaaa cggagcaggt ttgctaccaa
    16621 gttatgaaaa tttttcactt atacttgaaa aacctcctcc tacaattggt tatttgacaa
    16681 ttgattttcc gaacacaggt aggagtccga ttcatgacca agctggaaaa aatctagata
    16741 atcttgcaga tgcggtttat gaagtacttg aagaattagg gatttctgaa tatatacttt
    16801 gtgcacatag ttggagtgga attttagctt gcaaattact cgagaaacca attaagcgtc
    16861 agactttagt agcaattgaa ccgacaacta aaaaagtcat gtttgctgat ttttcagaaa
    16921 atccttatcc agaaatggaa gagcagatga ggctgattga cgagtgtggt cctgaacttt
    16981 attttaagaa cttaactcaa gcaacattta gtcctgaaac gaataaaaaa atctgggaat
    17041 taatgcaaga aaaaggctta gagttggaaa atcaagatcc agaatttcag atatctggag
    17101 agattactga ggaagatttt gagaatttgt cgatagaatc tcatgtccct gtatttattt
    17161 tttgtcagac ttatagagaa aaagagtaca gagaatcaga atattggact tccaatacta
    17221 aactcatttt aggagggaat caccattatt tacaatggtc agaatcggaa aaaattgcgg
    17281 ctattattcg agaattgtca gaataagatg gaaaaaagga gattacagga gacaagatga
    17341 actactttaa tgttgggaaa atcgttaata cgcagggatt acagggtgag atgcgagtct
    17401 tgtctgtgac ggattttgca gaagaacggt ttaaaaaagg agctgagctg gctttgtttg
    17461 atgaaaaaga tcagtttgtc caaacagtga ccatcgctag ccaccgtaaa cagaagaact
    17521 ttgacattat taaattcaaa gatatgtacc atatcaatac tatcgaaaag tacaagggat
    17581 acagtctcaa ggtcgctgag gaagatttga atgacctaga cgatggtgaa ttttactatc
    17641 acgagattat cggtttggaa gtctatgagg gtgatagctt ggttggaacc atcaaggaaa
    17701 tcctgcaacc aggtgctaat gatgtctggg tggtcaaacg aaaaggcaaa cgtgatttgc
    17761 ttttacctta tatcccacca gtggttctca atgttgatat tccaaataaa cgggtcgatg
    17821 tggaaatctt agaagggtta gacgatgaag attgatattt taaccctctt tccagagatg
    17881 ttttctccac tggagcactc aatcgttgga aaggctcgag aaaaagggct cttggatatc
    17941 cagtatcata attttcgaga aaatgctgaa aaggcccgtc atgtagatga tgagccctac
    18001 ggaggcggtc agggcatgtt gctcagagca caacctattt tcgattcctt tgatgctatt
    18061 gaaaagaaaa atccgcgcgt tattctcctt gatcctgctg gaaagcagtt tgatcaggct
    18121 tatgctgaag atttggctca agaggaagag ctaatcttta tctgtgggca ctatgagggt
    18181 tatgatgagc gcattaagac cttggtaaca gatgagattt ccctaggtga ctatgtcctc
    18241 actggtggag aattggcagc tatgaccatg attgatgcta cagttcgcct gattccagaa
    18301 gtgattggca aggagtctag ccaccaagat gatagttttt cttcaggtct tttagaatat
    18361 cctcagtaca cacgtcccta tgattatcga ggcatggtcg tgccagatgt attgatgagt
    18421 ggccatcatg aaaagattcg tcagtggcga ttgtacgaga gtttaaagaa aacctacgag
    18481 cgcagaccgg atttacttga acattatcaa ctgacagtag aagaagaaaa aatgctggca
    18541 gaaatcaaag aaaacaaaga ataaaggaga aacctatgca agtaatcaaa cgtaatggcg
    18601 aaattgctga atttaatcca gataagattt accaagccat cttgaaggca gcccaaactg
    18661 tttatgtttt gacagatgat ttgcgtcaaa atcttgctca agtcactaag aaggtagttt
    18721 tggatttaca agaagccaag gtggaacgtg cgactatcag tatgattcaa tctatggttg
    18781 aacatcgttt attgggcgca ggttacatta ccattgcaga acactatatt tcctatcgtt
    18841 tacaacgtga cttggaaaga agtggttatg gagatcatat cgcagttcat ttacattttg
    18901 aacaaattcg ctaagaaaaa gagtgggatg aagcaactac atctcactct ttcttaaatc
    18961 tatttttttg ggctgtttgg acagttgagg ggacaaatcg caggcgttga atatcgctta
    19021 tacggataat ccggctcaca tttttggaaa aatttttcac gataatttgc tggccttgct
    19081 gatcatattt gatgatgtca cctgtaaagc ttgtctcaga taagataagg tgaacagcgg
    19141 tttgtttctg gatagcctct tcaatagtgg ctgtaaggga gttggtttca gtctggtcag
    19201 gttggtcata tccatttaaa aaatcatgta ttttatttag aacttgctgg aatttatgtc
    19261 tcatattggc ctcccttctt ttatatcaat attatataaa attttcctaa aatctacaag
    19321 attttacgaa taaactaatg aaactaaaaa aggagaagaa aatggcagaa tttacatttg
    19381 aaatcgaaga gcacttgttg actctttctg aaaacgaaaa aggttggacc aaggaaatta
    19441 accgtgtgag ctttaatggt gcccctgcaa agtttgatat tcgtgcttgg agtccagacc
    19501 atactaaaat gggcaaaggg attactctct caaatgaaga atttcaaacg atggtggatg
    19561 cctttaaagg caactaatac tcttcgaaaa tctcttcaaa ccgcgtcagc tttgccttgc
    19621 cgtatgtatg gttactgact tcgtcagttt catccacaac ctcaaaacag tgttttgagc
    19681 agcctgcggc tagcttccta gtttgctctt tgattttcat tgagtataag ttttcaaaat
    19741 aaaagaaaag gatattgagt catgataact cgatatcctt ttttagttag taaagtaagc
    19801 ctgattttta aggcgttgaa gtaggggacg gctaataaaa ctaatagcca gaagtttgcc
    19861 aaggtctgga atgataaagg gaagaacccc cacagcaaga gctttttcaa atgccattcc
    19921 agctaggaaa tgcaagctga gaatcccgcc gacaaagaca agggcatcac ccaagaggtt
    19981 tgcaagaaaa atcttaacaa caccactctt gctgttggtt agagaggaag taagtccaga
    20041 gtaaacgaga taaaaccaaa gatagcctgc agtagggcca actaaagcct gaaaaccagc
    20101 tccacctcct gcaaagacag gaagaccgat agcacctaga agaagataga gtccagcaga
    20161 aagtacagcc tctctcggtc taaagacagt agcaatcaag ccgattgcaa agttttgcag
    20221 agtgaaggga acaggtccaa ttggaagact gatttgtgcc aaagcagcaa tgagagcagc
    20281 cccaatagca gggatagcat aaacgtgagc ttttttcaaa atagttaacc tcttttctaa
    20341 tgtatagtaa ctattatact catataggag tcgatgtcaa ccatttttta aaaacaaggt
    20401 aacaaaaact gtaacagccg attttcgtga ctagtcaagt tggataattt ttttgttgat
    20461 tcgaaaatca ttaaaaaaga agcagacata tttatatcag cttcttttgt ttcgattaac
    20521 gcatggttac aaattcttca gatgcagttg ggtgaattgc cacagttgca tcaaagtcag
    20581 ccttggttgc tcccattttg atagcaacgg caaatccctg aatcatttca tcaacgccgt
    20641 agccaattcc atgaagtccg acaacttttt cttctgaacc agctgttatg agtttgaaac
    20701 gggattcttg acggttgcga gtgcaagcag agtacataga tgcaaagctt gatttataaa
    20761 ccttgatttg gtcttgaccg tattctttaa tagcttgctc ttctgtcaaa ccaacagttc
    20821 cgatagcagg gtgtgaaaag acaacagttg gaatagttga gtaatccatt tttgcagtag
    20881 tttttccgtt aaagagacgt tcagataggg tacgtccggc cttgattgca actggagtca
    20941 gttctttctc gcccgttaca tcacctagag catagattcc ctcaacaaca gtattttggt
    21001 attcatccac ttggataaag ccacgttcgt tcagagtcac tccagctttt tcaagttgca
    21061 agcccttaac gtttggacgg cgacctgtag cccagataac ttggctagct gtgtgactag
    21121 taccatcttc gaaatgaatg gtaatgccgt cagtagtttt ttctaacttg acagggactt
    21181 tgtgagtgtg aagtggtaag tttgttcttt ccatttcctt gaccaaacct tcaacgatgt
    21241 aggaatcaaa accacgtaaa ggacgatcgc ggcgaacaaa gagatctgtc ttgacaccaa
    21301 aagtgtggag tacgccagct aattcaacgg cgatataacc agcgcctaga atggcaattg
    21361 actctggaag ttcttcccag gcaaatacat catcagaaga gccacctagc tcagcaccag
    21421 gaatattagg aatacttgga tgggcacctg tagcaatcac gatatgttta gcacgaatca
    21481 gttcaccatt tacgcttaca gtatgagaat ctacaaattc agcatgacct tcaatcaagt
    21541 ctacaccgtt gcgtttaaaa ctaccatcat aagaagaacg agcgcgatca atgtaggctt
    21601 cacgattgcg acgtagggtt gcaaagttaa agttaagatc agtagtctta aagccgtagt
    21661 cttctccaaa ttgatggaaa gtctcagcga tttgcgcccc gtaccacatg atttttttag
    21721 gaacacaacc gacgttgaca caggttccac ctaatttctt ttcctcaata acggctgctt
    21781 tggctccatg ttcaccagca cggttcatgg tagcgattcc tccgctacct ccaccgatag
    21841 caatgatatc atattctctc attgaaaact cctttgtaat cttttaaata gtagagtgtc
    21901 ttttttgata gttatatttt attctttttt tgatttgatt gcaaaaatct gctacgcaca
    21961 aaaaattgca aaaaagcttg ccaaataagt caagatagtg tattatatat ctagtacaat
    22021 agaaaagcaa aattccgaac gattgtttaa tcctgaactg ccattatttc catctgaact
    22081 gcattgtttg ctggagggaa atttggtata atagcaaagt attgtaaaaa agaaaaggag
    22141 tttcattatg aagaaaaaga atggtaaagc taaaaagtgg caactgtatg cagcaatcgg
    22201 tgctgcgagt gtagttgtat tgggtgccgg ggggatttta ctctttagac aaccttctca
    22261 gactgctgta aaagatgagc ctactcatct tgttgttgcc aaggaaggaa gcgtggcatc
    22321 ctctgtttta ttgtcaggga cagtaacagc aaaaaatgaa caatatgttt attttgatgc
    22381 tagtaagggt gatttagatg aaatccttgt ttctgtgggc gataagatca gcgaagggca
    22441 ggctttagtc aagtacagta gttcagaagc gcaggcggcc tatgattcag ctagtcgagc
    22501 agtagctaag gcagatcgtc atatcaatga actcaatcaa gcacgaaatg aagccgcttc
    22561 agctccggct ccacagttac cagcgccagt aggaggagaa gatgcaacgg tgcaaagccc
    22621 aactccagtg gctggaaatt ctgttgcttc tattgacgct caattgggtg atgcccgtga
    22681 tgcgcgtgca gatgctgcgg cgcaattaag caaggctcaa agtcaattgg atgcaacaac
    22741 tgttctcagt accctagagg gaactgtagt cgaagtcaat agcaatgttt ctaaatctcc
    22801 aacaggggcg agccaagtta tggttcatat tgtcagcaat gaaaatttac aagtcaaggg
    22861 agaattgtct gagtacaatc tagccaacct ttctgtaggt caagaagtaa gctttacttc
    22921 taaagtgtat cctgataaaa aatggactgg gaaattaagc tatatttctg actatcctaa
    22981 aaacaatggt gaagcagcta gtccagcagc cgggaataat acaggttcta aataccctta
    23041 tactattgat gtgacaggcg aggttggtga tttgaaacag ggtttttctg tcaacattga
    23101 ggttaaaagc aaaactaagg ctattcttgt tcctgttagc agtctagtaa tggatgatag
    23161 taaaaattat gtctggattg tggatgaaca acaaaaggct aaaaaagttg aggtttcatt
    23221 gggaaatgct gacgcagaaa atcaagaaat cacttctggt ttaacgaatg gtgctaaggt
    23281 catcagtaat ccaacatctt ccttggaaga aggaaaagag gtgaagactg atgaagcaac
    23341 taattagtct aaaaaatatc ttcagaagct accgtaatgg tgaccaagaa ttgcaggttc
    23401 tcaaaaatat caatctagaa gtgaatgagg gtgaatttgt agccatcatg ggaccatctg
    23461 ggtctggtaa gtccactctg atgaatacga ttggcatgtt ggatacacca accagtggag
    23521 aatattatct tgaaggtcaa gaagtggctg ggcttggtga aaaacaacta gctaaggttc
    23581 gtaaccaaca aatcggtttt gtctttcagc agttctttct tctatcgaag ctcaatgctc
    23641 tgcaaaatgt agaattgccc ttgatttacg caggagtttc gtcttcaaaa cgtcgcaagt
    23701 tggctgagga atatttagac aaggttgaat tgacagaacg tagtcaccat ttaccttcag
    23761 aattatctgg tggtcaaaag caacgtgtag ccattgcgcg tgccttggta aacaatcctt
    23821 ctattatcct agcggatgaa ccgacaggag ccttggatac caaaacaggt aaccaaatta
    23881 tgcaattatt ggttgatttg aataaagaag gaaaaaccat tatcatggta acgcatgagc
    23941 ctgagattgc tgcctatgcc aaacgtcaga ttgtcattcg ggatggggtc atttcgtctg
    24001 acagtgctca gttaggaaag gaggaaaact aagatgcaga atctgaaatt tgccttttca
    24061 tctatcatgg ctcacaagat gcgttctttg cttactatga ttgggattat tatcggtgtt
    24121 tcatcagttg ttgtgattat ggctttgggt gattccctat ctcgtcaagt caataaagat
    24181 atgactaaat ctcagaaaaa tattagcgtc tttttctctc ctaaaaaaag taaagatggg
    24241 tcttttactc agaaacaatc agcttttacg gtttctggaa aggaagagga agttcctgtt
    24301 gaaccgccaa aaccgcaaga atcctgggtc caagaggcag ctaaactgaa gggagtggat
    24361 agttactatg taaccaattc aacgaatgcc atcttgacct atcaagataa aaaggttgag
    24421 aatgctaatt tgacaggtgg aaacagaact tacatggacg ctgttaagaa tgaaattatt
    24481 gcaggtcgta gtctgagaga gcaagatttc aaagagtttg caagtgtcat tttgctagat
    24541 gaggaattgt ccattagttt atttgaatct cctcaagagg ctattaacaa ggttgtagaa
    24601 gtcaatggat ttagttaccg ggtcattggg gtttatacta gtccggaggc taaaaggtca
    24661 aaaatatatg ggtttggtgg cttgcctatt actaccaata tctcccttgc tgcgaatttt
    24721 aatatagatg aaatagctag tattgtcttt cgagtgaatg ataccagttt aaccccaact
    24781 ctgggtccag aactggcacg aaaaatgaca gagcttgcag gcttacaaca gggagaatac
    24841 caggtggcag atgagtccgt tgtatttgca gaaattcaac aatcgtttag ttttatgacg
    24901 acgattatta gttccatcgc agggatttct ctctttgttg gaggaactgg tgtcatgaac
    24961 atcatgctgg tttcggtgac agagcgcact cgtgagattg gtcttcgtaa ggctttgggc
    25021 gcaacacgtg ccaatatttt aattcagttt ttgattgaat ccatgatttt gaccttgtta
    25081 ggtggcttaa ttggcttgac aattgcaagt ggtttaactg ccttagcagg tttgttactg
    25141 caaggtttaa tagaaggtat agaagttgga gtatcaatcc cagttgccct atttagtctt
    25201 gcagtttcgg ctagtgttgg tatgattttt ggagtcttgc cagccaacaa ggcatcgaaa
    25261 cttgatccaa ttgaagccct tcgttatgaa tgagatcaac aagatggaca ctcgtctatc
    25321 ttgtttttgt atggatttcc ctatcgaaat tcattaaaaa tgtgatataa tgaagtgtta
    25381 gaaaaacagg tttcatttgc ctggaaagga aaaattatgt ctgaaaaaaa tttttatatt
    25441 acaacgccga tttactatcc atctgggaaa cttcatatcg gttctgccta cacaactatc
    25501 gcatgtgatg tcctagcacg ttacaaacgc ctgatgggct acgatgtatt ttatctgaca
    25561 ggtcttgatg aacatggtca gaaaatccag cagaaagcgg aagaagctgg tattacacct
    25621 caagcctatg ttgatggaat ggcggttgga gttaaagaac tctggcaatt actagatatc
    25681 tcatacgata aatttatccg tacaactgat gactaccatg aaaaagttgt cgcacaagtc
    25741 tttgaacgct tacttgctca agatgatatc tacttgggtg aatactctgg ttggtattca
    25801 gtatcagacg aagaattctt tacagaaagc cagctggcag aagttttccg tgatgaagct
    25861 ggaaatgtga ctggtggtat tgctccatca ggtcatgagg ttgaatgggt ttctgaagaa
    25921 tcatacttcc ttcgccttag caaataccaa gaccgtttgg tcgaattttt caaagctcat
    25981 cctgaattta tcacgccaga gggtcgcctt aatgaaatgc tacgcaactt catcgagcca
    26041 ggtttggaag atttggcggt atctcgtaca acctttacat ggggagtgcc tgtcccatca
    26101 aatccaaaac acgttgtcta cgtttggatt gatgcccttc ttaactatgc gacagctctt
    26161 ggctacgctc aagacgaaca tggtaacttt gacaagttct ggaatggaac agtcttccat
    26221 atggtaggaa aagatatcct tcgcttccac tctatctact ggccaatcct tcttatgatg
    26281 ttggatgtta aattacctga tcgtttgatt gcccatggtt ggtttgtcat gaaagacgga
    26341 aaaatgtcta agtcaaaagg gaatgtcgtt taccctgaaa tgttggtaaa gcgttatgga
    26401 ctagatccac ttcgttacta cctcatgcgt aaccttccag ttggttcaga cggaaccttt
    26461 actcctgaag actatgtcgg ccgtatcaac tatgaattgg ctaatgacct tgggaacctc
    26521 cttaaccgta cggtttccat gattaataag tactttgatg gacaaatccc tgcctatgta
    26581 gaaggtgtga ctgaatttga tcatgttctt gctgaggttg cagaacaatc aatcgcagac
    26641 ttccatacac acatggaagc agttgactat ccacgtgcgc ttgaagcagt ctggactctg
    26701 atctctcgta ccaataaata catcgatgag accgcaccat gggtcttggc caaggatgaa
    26761 gctcttcgtg accaattggc aagtgtcatg agccacttgg cagccagcat tcgtgtagtt
    26821 gctcacttga ttgaaccatt tatgatggaa actagtcgtg cagttttgac tcaacttggt
    26881 ttggaagaag tttctagtct tgaaaacttg agtttggctg acttcccagc agatgtgact
    26941 gtagttgcca aaggaacacc tatctttcca cgtctaaata tggaagaaga aatcgcctat
    27001 atcaaggaac aaatggaagg caataaacca gcagtcgaaa aagaatggaa tccggacgaa
    27061 gttgagctca aactaaacaa ggatgaaatc aagtttgaag actttgacaa ggttgaaatc
    27121 cgtgtcgcag aagtcaaaga agtgtctaaa gtagaaggtt cagataagtt gcttcaattc
    27181 cgcttggatg ctggtgatgg agaagatcgt cagattcttt caggaattgc aaaatactat
    27241 ccaaatgaac aagaattggt cggcaagaaa gttcaaatca ttgctaacct caaaccacgt
    27301 aaaatgatga aaaaatatgt cagccagggt atgattctct cagctgaaca tgatggcaaa
    27361 ttaacccttc tcacagttga tccagctgta ccaaatggaa gtgtgattgg gtaaaagtag
    27421 acaggactag ttcaggctag ttctgttttt ttatttatac tcaatgaaaa tcaaagagca
    27481 aactaggaag ctagccgcag gt
//
LOCUS       DAVKHZ010000020        25815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000020 DAVKHZ010000000
VERSION     DAVKHZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.020"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..201)
                     /locus_tag="VWW99_000798"
     CDS             complement(<1..201)
                     /locus_tag="VWW99_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU9538673.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTGTWQGAATGAAGGAILGG"
     gene            complement(270..539)
                     /gene="blpJ"
                     /locus_tag="VWW99_000799"
     CDS             complement(270..539)
                     /gene="blpJ"
                     /locus_tag="VWW99_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9538674.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLVGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1006..1203)
                     /gene="blpI"
                     /locus_tag="VWW99_000800"
     CDS             complement(1006..1203)
                     /gene="blpI"
                     /locus_tag="VWW99_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9538675.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1485..2072
                     /locus_tag="VWW99_000801"
     CDS             1485..2072
                     /locus_tag="VWW99_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9538676.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1999..2742
                     /locus_tag="VWW99_000802"
     CDS             1999..2742
                     /locus_tag="VWW99_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin ABC transporter"
                     /protein_id="HEU9538677.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALTSTILSLFLDVSILILVEGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGNKISSEYPYPMVWRSISHVE"
     gene            2732..3643
                     /locus_tag="VWW99_000803"
     CDS             2732..3643
                     /locus_tag="VWW99_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9538678.1"
                     /translation="MSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQSAKVANNRL
                     NEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIKQGDKVSLV
                     GVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQAYIFNGSI
                     LENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGGQKQRIALA
                     RALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAERTNRVIVL
                     DQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            3654..5015
                     /locus_tag="VWW99_000804"
     CDS             3654..5015
                     /locus_tag="VWW99_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9538679.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5072..5227
                     /gene="blpC"
                     /locus_tag="VWW99_000805"
     CDS             5072..5227
                     /gene="blpC"
                     /locus_tag="VWW99_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9538680.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FYQPIQL"
     gene            complement(5271..6611)
                     /locus_tag="VWW99_000806"
     CDS             complement(5271..6611)
                     /locus_tag="VWW99_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9538681.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGVSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(6625..7362)
                     /locus_tag="VWW99_000807"
     CDS             complement(6625..7362)
                     /locus_tag="VWW99_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538682.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(7367..7705)
                     /locus_tag="VWW99_000808"
     CDS             complement(7367..7705)
                     /locus_tag="VWW99_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538683.1"
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVKIVTEEASFNM
                     VQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7982..8311
                     /locus_tag="VWW99_000809"
     CDS             7982..8311
                     /locus_tag="VWW99_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9538684.1"
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(8479..9528)
                     /locus_tag="VWW99_000810"
     CDS             complement(8479..9528)
                     /locus_tag="VWW99_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538685.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVEVVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(9525..10256)
                     /locus_tag="VWW99_000811"
     CDS             complement(9525..10256)
                     /locus_tag="VWW99_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538686.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            10324..10734
                     /locus_tag="VWW99_000812"
     CDS             10324..10734
                     /locus_tag="VWW99_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9538687.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            10744..11031
                     /locus_tag="VWW99_000813"
     CDS             10744..11031
                     /locus_tag="VWW99_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538688.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            11193..11369
                     /locus_tag="VWW99_000814"
     CDS             11193..11369
                     /locus_tag="VWW99_000814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538689.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDTTHIYTTRQR"
     gene            complement(11398..12534)
                     /gene="dnaJ"
                     /locus_tag="VWW99_000815"
     CDS             complement(11398..12534)
                     /gene="dnaJ"
                     /locus_tag="VWW99_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9538690.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGGLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12936..13292)
                     /locus_tag="VWW99_000816"
     CDS             complement(12936..13292)
                     /locus_tag="VWW99_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9538691.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(13294..15117)
                     /gene="dnaK"
                     /locus_tag="VWW99_000817"
     CDS             complement(13294..15117)
                     /gene="dnaK"
                     /locus_tag="VWW99_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9538692.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAESDKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(15597..16121)
                     /gene="grpE"
                     /locus_tag="VWW99_000818"
     CDS             complement(15597..16121)
                     /gene="grpE"
                     /locus_tag="VWW99_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9538693.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(16148..17182)
                     /gene="hrcA"
                     /locus_tag="VWW99_000819"
     CDS             complement(16148..17182)
                     /gene="hrcA"
                     /locus_tag="VWW99_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9538694.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QDSCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(17346..17855)
                     /locus_tag="VWW99_000820"
     CDS             complement(17346..17855)
                     /locus_tag="VWW99_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9538695.1"
                     /translation="MNSRVEFRIFTILDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQEFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREELSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            18462..20795
                     /locus_tag="VWW99_000821"
     CDS             18462..20795
                     /locus_tag="VWW99_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9538696.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20808..22271
                     /locus_tag="VWW99_000822"
     CDS             20808..22271
                     /locus_tag="VWW99_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9538697.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            22271..23839
                     /locus_tag="VWW99_000823"
     CDS             22271..23839
                     /locus_tag="VWW99_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9538698.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLAEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            23896..24693
                     /gene="psrA"
                     /locus_tag="VWW99_000824"
     CDS             23896..24693
                     /gene="psrA"
                     /locus_tag="VWW99_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9538699.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            24704..25303
                     /locus_tag="VWW99_000825"
     CDS             24704..25303
                     /locus_tag="VWW99_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9538700.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQL"
     gene            complement(25257..>25815)
                     /locus_tag="VWW99_000826"
     CDS             complement(25257..>25815)
                     /locus_tag="VWW99_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9538701.1"
                     /translation="NKDETTSYPIYKIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
BASE COUNT         8317 a         5063 c         4641 g         7794 t
ORIGIN      
        1 acctccaagt atagctccac ccgcagcacc agttgctgca ccttgccatg ttcctgtttt
       61 aatgcctagt tgaagacctc ttgctgctcc tcctccaaca cctgctttgg caaaatctcc
      121 ccaattgcat ccgccacctt caacgcaagc aagcatttca gtatccataa cagaaaattg
      181 tgacatcatt tttgtatcca tgacaaatac tcctttttta aaaaactaaa ataaatcaga
      241 atagaatcct cataatttta ctataagtct taccaactta gtcccaattt atcaccaacc
      301 atacctccta agcatgttaa tccaccccca attgcaccaa tgtgtgctcc aacaaatgca
      361 ccaacaagtc cagctactcc taaagtggcc aaacctgctc cagttccacc agttataatt
      421 cccgtagtga ctcctgtaat cagtgcattt tgacaatcag tggagctata ccccccttca
      481 actttcgcaa gcatttcagt atccataacc tctaactgtg acaacatttt tgtattcatg
      541 atgaatacct cctttttatt ttcaatttgt taccaaagtc ttaaattcaa taaacaaata
      601 gattttttat agtatctttt tgattttctt aaaaaagtat atacgtctac tatcctctta
      661 aaggtagcag tacctatttt ttagtctaag atttcaataa tcttgagtat ctaaaatatc
      721 ttaatttcgt tattctcctt gcaataaaaa gttttactat actatttatt aacttgcaga
      781 aagcaaaaaa tattagtaaa taatagttta tagttaagtt ttttattcct accaatccat
      841 caactaagta aagcatcaac gattacataa acgattgata atataattaa aattttgcta
      901 actatcttat tctcatcatt cttagataac tttgatattt tgtaagtaag taaataagac
      961 agtaaattaa tagcgataat aatactatat ttaagaatca taatcttaca aagaggacat
     1021 aattcctgaa cctacacaaa taagtgttgc tgctccccca gttatcggac cagtcgcagc
     1081 agctaatagt actgctccaa tacaaccacc gattgcagat cctaaattgc ctcttcctcc
     1141 actaactatt tcgagttctt cattatccat aacagaaaat tgttccatca tttttgtatt
     1201 catgacaaat actccttttt tcttttttta tttttgtctt gttgtaactt tgataagttt
     1261 agtatatcat cgttttttaa aatttttcat ccagatcttg aattgtcatc gaaacgtctt
     1321 gaattaggtt ttttatttca agccacctct aaatgtttaa aaaaaataat ttctaatcac
     1381 ttttttacca ttcaggaagt tttaatgact attcaagatt tcataaaata tgaacttagt
     1441 tttatgacat aatagaccta tccactatat gaaaggaatt gccaatgact tcttataaac
     1501 gtacatttgt tcctcaaata gatgcgagag actgtggtgt cgctgcctta gcctcgattg
     1561 ctaaattcta tggttcagat ttttctctag ctcacttgag agaacttgca aagaccaata
     1621 aagaagggac gactgctctt ggcattgtaa aagccgctga tgaaatgggc tttgaaacaa
     1681 gacctgttca agcagataaa acgctctttg acatgagtga tgtcccctat ccatttatcg
     1741 ttcacgttaa caaagaagga aaactccaac attactatgt tgtctatcaa acaaagaaag
     1801 actatctgat tattggtgat cctgaccctt ctgtaaaaat cactaaaatg tcaaaagaac
     1861 gctttttcta tgaatggact ggagtagcta tttttctagc taccaaaccc agctatcaac
     1921 cccataaaga taaaaagaat ggtctactaa gcaagcttcc ttcctctgat tttcaaacaa
     1981 aaatctctca ttgcttacat tgttctctca agcttattgg tcactattat caatataggt
     2041 ggttcttact atctccaagg aatcttggat gaatacattc caaatcagat gaaatcaact
     2101 ttaggaatca tctcagttgg tctggttatc acctatatcc tccaacaagt catgagcttc
     2161 tccagagatt atctcctaac cgttctgagt cagagattaa gtattgatgt gattttatcc
     2221 tatattcgcc atatttttga acttcccatg tctttctttg cgacacgtcg tacaggagaa
     2281 atcatttcac ggttcacaga tgctaactct attatagatg ccttgacttc taccattctt
     2341 tctctttttc tggatgtttc tattctgatt cttgtagagg gagtcttgct ggcacaaaac
     2401 cctaatctct tccttctttc tcttatttcc attcctatat acatgttcat catcttttct
     2461 tttatgaagc ctttcgaaaa aatgaaccat gatgtcatgc aaagtaattc tatggttagc
     2521 tctgccatta tcgaagatat caacgggatt gaaactataa agtcgctcac gagtgaagaa
     2581 aatcgctatc aaaatataga cagcgaattt gtagattatt tggaaaaatc ctttaagctc
     2641 agtaaatatt ctattttaca aactagttta aagcagggga acaaaattag ttctgaatat
     2701 ccttatccta tggtttggcg ctcaattagt catgtcgagt aaaatttcta tcggtcagct
     2761 gattaccttt aacacacttc tttcttactt tacaactcct atggaaaata ttatcaacct
     2821 ccaaaccaaa ctccaatctg cgaaggttgc taataaccgt ttgaacgaag tctatctagt
     2881 cgaatctgaa tttcaagttc aagaaaaccc tgttcattca cattttttga tgggcgatat
     2941 tgaatttgat gacctttctt ataagtatgg ttttggacga gataccttaa cagatattaa
     3001 tctcacgatt aaacaaggag ataaggttag cctagttgga gttagtggtt ctggtaaaac
     3061 aactttagcc aaaatgattg tcaatttctt tgaaccctac aaaggacata tttccatcaa
     3121 tcatcaggat attaaaaaca ttgataaaaa agtcttgcgc cgtcatatta attacctacc
     3181 ccaacaagcc tatatcttta atggctctat cttggaaaac ttaactttgg gcggtaatca
     3241 tatgattagc caagaagata ttctaagagc ttgtgaatta gctgaaattc gtcaagacat
     3301 tgaaagaatg cctatgggct atcaaactca gctctctgat ggagctggtc tatcaggagg
     3361 acaaaagcaa cgaatagccc tcgctcgtgc tcttttaact aaatctcctg ttttaatact
     3421 agacgaagct actagcggtc ttgatgtctt gactgagaaa aaggttatag ataatcttat
     3481 gtctctaact gataaaacca ttctctttgt agcccatcgt ctcagtatag ccgaacgaac
     3541 caaccgtgtc attgttcttg accaggggaa aatcattgaa gttggtagtc accaagagtt
     3601 aatgcaggcg caaggtttct accatcatct attcaataaa taaggagaat gtcatgaatc
     3661 ctaatctttt tagaagcgtc gagttttatc agagacgtta ccataactat gcgacagtgt
     3721 taattatacc tctttcatta ctatttactt tcatcttgat tttctccctt gttgccacaa
     3781 aagaaattac tgttacttcc caaggagaaa tcgcccctac aagtgtcatt gcctccattc
     3841 agtcaaccag tgataatcct atcctagcta atcatttagt ggcaaatcaa gtagttgaaa
     3901 aaggggactt actcatcaaa tactctgaaa caatggaaga aagtcagaaa actgccttag
     3961 caactcaatt acaaagactt gagaagcaaa aagaaggact tggaattttg aaacaaagct
     4021 tagaaaaagc gactgatctt ttttctggcg aggatgaatt tggctaccat aataccttta
     4081 tgaattttac taaacaatcc catgatattg aactgggtat cacaaagact aacaccgaag
     4141 tttcaaatca agctaatctt tccaatagca gttcatcagc tattgaacaa gaaattacaa
     4201 aagttcaaca acaaattgga gaatatcaag agttgagaga tgctatcata aataacagag
     4261 cacgcttacc aactggcaat ccgcaccagt caattttgaa tcgttatctt gtagcctcac
     4321 aaggacaaac acaaggaact gcagaggagc catttttatc tcaaattaat caaagtattg
     4381 caggtcttga atcatctatc gcaagcctca aaattcagca agctggtatc ggaagtgtag
     4441 caacttatga taacagttta gcaaccaaaa ttgaagtact ccgcactcag tttttacaga
     4501 cagcctcaca gcaacaacta actgtggaga atcaattaac agaattaaaa gtacaactag
     4561 atcaagccac acagcgtttg gaaaacaata ccttaacctc cccaagtaaa ggtatcgttc
     4621 atctgaacag cgaatttgaa ggtaaaaata gaattccaac tggtacagaa attgctcaaa
     4681 tattccctgt catcacagat acaagagaag tactaatcac ttactacgta tcttctgact
     4741 atctacctct actagataaa ggacaaactg taagattaaa actggagaag attggaaatc
     4801 acggcaccac catcatcggc caacttcaga caattgatca aactcctacc agaacagagc
     4861 aaggaaatct ctttaaatta accgctcttg caaaactatc taacgaggat agtaaactca
     4921 tccaatatgg cttacaaggt cgcgtcacta gtgtaactac aaagaaaaca tattttgatt
     4981 atttcaaaga taaaatttta acacattctg attaattttc agataacact ctataactat
     5041 ttattatctt atcaaaaagg agaatcataa catggataag aaacaaaacc taacttcatt
     5101 tcaagaacta acaactaccg aactcaacca aattataggt ggaggatggt gggaagattt
     5161 cttatataga tttaatataa ttgaacaaaa aaatacaaaa ggattttatc agccaataca
     5221 actataaaaa aacaagacca agagaacgca ctctcggtct tattttttct tcattctata
     5281 tgtattacag taagtacctg acgaaaaacg tgatcttggc aggtggtatt gagactggta
     5341 ttgggatgac tttccacaat tttcataacg gtatagagac caactcctct ctcctcccct
     5401 ttagaacttg ctccaaagga gaagatttca gaaatatcga ttccctcttc tttgatggaa
     5461 ttttcaatga taaaggtctc ctgtgctcca tttttaaaaa aggcaattga aacatgaggt
     5521 tgacaggctt ctacactagc ttcaatagcg ttgtcacaaa ggatagacac aacggttaga
     5581 aaatcaagta gactcacccc ctctacctga atctcctctg gaacttcgac attaaagaca
     5641 atgttcttat ctctagcttt tataaatttt ccagctagga gacttttgag ggcacgatcc
     5701 cgaacattca ccaatcggcc aaggtcatat ttattgtcct gcaatttttc actagaatcc
     5761 tttaagaccg agtcgtagat ctcttttatc tgctccatat cctcctcttc aatgcccaga
     5821 cgtaagctag tcaagaggtt ggtgtagtca tggcgaaaac tccgtacttc cttataaagc
     5881 tcctctatat gccgactata ccgctccatc tctctgtagc gcaagtcctg ctcttggttc
     5941 agtctctcat ggagtttgtc cttcaaatag gtatccaatt tcttgataat ccccataaaa
     6001 aagagcaggt aaaagactag gatgagatgg cgaacagtcg ttgattgaat cccctgttca
     6061 tattcaaagt aagacagatt ttgtatcacc aaatagtaag cccccattat ccagttaatc
     6121 tgagtcaggg acttttgaaa atctttatcg agaatcccct ttctcaagct agtgaaatca
     6181 tagtccaacc atttcaaaaa agctagagaa atgaagaaat tgaaaattat tatacataac
     6241 caaataaatg agtagtcatc atatacttgc ccttgtccca aaaatggaag cacaaaatag
     6301 gaaacacctc tataaaagag attcactaat atcattggaa agagaccata aaagagaagg
     6361 agttttttag gaagttctct taacaataag aaagataagc ctatgccgta caagggttcc
     6421 ataaaataag ataggtaaac atttcctacc atatagccaa gcattccaaa aacaacggcc
     6481 aacagtatct taaaaagaaa ggccttaaaa atcctctcaa aagtgagact aattccatct
     6541 accttaaaga atatgacaat ttttaatccg tgagtaacaa gtgtatacaa caatatccaa
     6601 gcaacattca taaactctcc tagctcagtg taagttatta atagcctcag acacttccct
     6661 gaccttataa cgcgcgatta gacaacttcc accattggga aagaaaagca gtttttcttt
     6721 cttatctaaa tgcaccacat tggcaggatt gatgagaaaa gagcgatggc actgcaagag
     6781 acgaggctcc tgcttgagaa cctcctctag actcgctgta aattccagcc tgtctgtctt
     6841 ggtatagaga ataacacgat ggggtcttgt ggacgtttcg agatagtaaa cctctttaaa
     6901 aggatactgg aattgagcaa attttgattt aaagtaaaag caatcttccg ctagactttt
     6961 actgtcttga ccattggcat agaggagggc tgtctcgata cgagattcaa actcctctgc
     7021 cgaaagggcc ttatcaatat aatccagagc agacacttgg tagcgaaagg acaggggcat
     7081 aaactccgag tgagtcgtca caaagacaat cagggcatac ggatcccgat cccgaatctt
     7141 tctagccact tccagaccct tcatctcctc atttcgaatc tcaatatcca aaaagaatag
     7201 ctgatgggcc cccttctcat gcacctctgc cagcaattga tctggcttac caaaaacttc
     7261 aaaagaactc agagtgatat gatgttcctt caagagtttc tcaatcgtcg tttcaattct
     7321 agtctgttgg gaaaaatcat cttctaaaac aaatattctc atcttcttac tctccttctt
     7381 tcaaccattt ttgacgaatt tctctatagc gacgtctggc atacttgaca gcgtattgac
     7441 cttcttctcc aagaaaaagg attctttctt gaaaatcaat cgattttact ttatcaagat
     7501 tgaccaaaca atttcgatga catctcatca aggtttcccc atattggttc tcaagattac
     7561 ttaaattttg aaccatatta aaactagcct cttctgtaac aatctttaca gtatgggctt
     7621 tagttggatg tgtttggata tagtagacat cgtcgacgtt tactttatag actgttttct
     7681 gtgtctgaat aatcatatgt ttcattggat tctcctccta agaaaagaat aacagaaaaa
     7741 atctaacaca agcacaccca atcctaaaca gtctttgaaa gctcctaaaa gaattatttt
     7801 tctacaatat catgatgagc ttaccactat tttatcataa aattcactca gcgcaattca
     7861 agacatttca atgacaatta aagatttaaa ggcgaaaaag gagataaaag tgatagactg
     7921 acagtatcaa aacacagttg ctagaatcaa gactggcacc cagattagag gaggaattct
     7981 catgacagat acagacccta tcaaaagagc tcagactttg attactgact taaacaaagc
     8041 ctatcaagca tgcaaacagg caaccgctga cgacgtccgc tttcaggagc aattaaactc
     8101 tattcttggt tttctagcca aggctgaaac agtggataat cgattcttga ttgaattgga
     8161 aaaattttac cagacttcca gtcttctcat gggactcagc gctcttgatc cagatgctcc
     8221 aactcgcgcc gcttggcggg cctatgaccg cttccacttt gaccaagtca agaccaagtt
     8281 aatactcaat gagaatcaaa gagcaaacta ggaagctagc cgcaagctgt acttgagtac
     8341 ggtaagacga cgctgacgtg gtttgaagag attttcgaag agtataagtc tctacggacc
     8401 aaccattatc ctgtagtaac aaaaaagaag ttgagacaga ctgaactcaa cttctttttt
     8461 agtgtcgtat cagcaatgtt agtcctgcat ctgacgtttt acctgataag gcaaatacaa
     8521 gactagtaaa accaaaccag ctcctagtaa ggctagaagg gctagttttt cttggaaggt
     8581 aatcaaccca acaactactt ccacaagtaa aacaaaactg gtcaatcctc gaactacctc
     8641 ctgtaagcct ttttcctttt gccccttgtc cagcggaaag agttgggtca aatactggta
     8701 gtcaaaggca tgatagaggg ccagcaactg gaagagcaag aggtagttaa agagaactac
     8761 cactgctgtc gcaatccaag cttgctcgat aaaaacctgc gccagtacgg aaagtaagag
     8821 aagacgaaga ctgagagcaa agaggtcgcc atttcgcaga taagaacgca gatagagatt
     8881 ttgccaaatc ttcccaggca ccttctgaac agcctttaag ataaagtcca gataggcacg
     8941 acgcttgaca ctgtttgaaa ttcccttgac ctgcgtaaag agggcaaaga aacgaagcaa
     9001 gacttgctta cgcttgcttt cttgagaaat aagatagtcc cagtccagtc cagtttcagt
     9061 gaaaaatttg ctggcctttt gacgaaagtg gaaatatttt cctaccccca ataaaagcac
     9121 atagagcaga aaaactggca agccataacc cattgctaaa aacaagggcg caaataacag
     9181 caagaaaagg gtctgtacaa agagccaaaa gactagggaa atgccagttt gacgcttgag
     9241 atggagcttg atttcctctt ctccaactaa gagaaagagc ttgtctggag cctccatata
     9301 ggtggcaatt cctccccaaa gtaaaagtaa aacagacgta attcctacaa acaaaaggat
     9361 aggccaatga ttttcaggaa aatgttgtaa gagttgactg tactggtagg ctagaaagcc
     9421 caacaggaca agcaggaaca agacaaagtg gtcattgagc acatagcgca gataaccaag
     9481 acactcctta cgaaaggcct gctttctctt taaaaacaag tctttcatag atcctcctct
     9541 ttggtcagag ccaagtaaat atcattcaaa ctagcctcag gcatatcaaa ggcttcacgt
     9601 agttgcagga gattgccttt ggcacgcacc tctcccttgt gaagaatgac aaaggcatca
     9661 cacattttct ccgccgaatc cagcacgtgg gtactcatga gaatagactt gcccttttgc
     9721 ttctccactt ccaaaagctg aatcaaatca gaaatagcca gcggatcaag accaaggaaa
     9781 ggctcatcca cgatgaaaag acttggatcc accacaaaag cacagataat catgaccttc
     9841 tgcttcatcc cttttgaaaa atgaacaggg aaccagtcta atttctgttc caaacggaac
     9901 atttttaaca agggctctac tcgttcgaaa gccacttttt gctcaatacc gtaagccata
     9961 gcaaccgttt cgatatgctc tctgagggtc aattcctcat acagactagg cgtctcagga
    10021 atgtagccaa tctgcttgcg gtagctagtc gcatctcctt gcagagtcag gccattgata
    10081 ttgatggagc cactataagg tgccaacaga ccgataatct cattgatcgt cgttgatttc
    10141 ccagcaccat tgagaccaat caaaccgacc aactgcccac tttcaacagt aaaggacaca
    10201 tctttcaaaa caggaacatg aacatagcca cctgtcaggt ttttaatttc taacatattt
    10261 tctccaaatc tggtataatg tagctatatt atatcaaaat tcagtacagt agaggtagat
    10321 tttatgtcag attgcatttt ttgtaaaatc atcgcagggg aaattcctgc ttcaaaagta
    10381 tatgaagatg agcaggtcct tgcctttctt gatatctctc aagtaacact aggacacacc
    10441 ttggtcgtgc caaaagaaca ctatcgcaat cttttggaga tggatgctac gagcgccagc
    10501 caactctttg cccaagtacc aaaagtagct caaaaagtca tgaaagtcac taaggctgct
    10561 ggtatgaata tcatttccaa ctgtgaagaa gtcgctggtc aaacagtttt tcatactcac
    10621 gttcaccttg tgcctcgcta cagtgctgac gatgacctca agattgattt tatcgcccac
    10681 gaaccagact ttgacaaact tgctcaagtc gctgaaacca tcaaaaacgc ttaaggagtt
    10741 gctatgaaat tatccaacct actgctattt gcaggagctg cagccggaag ttatctggtt
    10801 acaaaaaatc gccaaaccat cacagatgaa gtcttgaata ccactgaccg cgttcaagct
    10861 atcaaggacg atgtggatat tatccaaaac agcctgcaaa tcattaacca gcaaaaagaa
    10921 cttatcaagg aataccaaga agacttgact tacaagttta aggtcttgga aaaggatatc
    10981 cagactagac tagctgtgat aaaagaaatg cagggaactg aagataagta aaaagagccc
    11041 cccggctctt ttttaaaacg taaacaagtt atagtgaatt gaatttagaa tagcacacca
    11101 tagtttctaa agcattgtta gagattactt taaaatcctc ttatcaattt gttcatattc
    11161 tatttcaatc tattatatat atcccatcaa ctatgcatca taatttaggt aactcatact
    11221 caataaaaat caaaaagcaa actagaaagc taggcacaga ctgctcaaaa caccgtttta
    11281 aggttgtgga tagaactgac gaagtcagct caaaacactg ttttgaggtt gtagatacga
    11341 ctcacatata tacgacaagg caacgctgac gtggtttgaa gaaatttttg aagagtatca
    11401 ttctccatca aaggcatctt taatatggtc aaagaagcct tttttctttg gatttacttt
    11461 caagccacca gcagccgcga attctttcaa ggctactttt tggcggtcgt tcaagcctgt
    11521 cggtgttacg acattaacag taacgtattg gtcaccaact gcaccgccac gaaggctcgg
    11581 tgccccctta ctacgtaggc ggaatttctt accagtctga gttccctctg gaataaccaa
    11641 ttcaacatca ccgtgaacag ttggaatatc tactgtatca ccaagagccg cttggacaaa
    11701 gttgaggttg agattgtaga agatagtcgt tccttcacgt tcaaacttgt cactagcttc
    11761 cacagaaact actacataca agtcaccata aggtccaccg ttaaagcctg cttcaccttg
    11821 accagcgagg cgaatttgtt gacctgtttc cacaccagca gggattttca catgtacgct
    11881 atgagcttgt ttctcatgac ctgttccatg acaggttgta catggatatt tgatttcttt
    11941 tcctcgaccg tgacagacat cacaggttac ttggcgacgc atcataccaa gaggagtctg
    12001 cgtatcgacg ttaatgacac cagcgccatg acagcgtcca caagtgactg gacttgtccc
    12061 tggcttagca ccagatccat tacatgtacg acagccagct tcacgatgat acttaacttc
    12121 cttctcagtt ccgaagatag cttcttcaaa ggtcaaattg acacgatact ggagatcatc
    12181 tccttggcga ggagcgtttg gattgcgcga agaaccgcct ccgccgaaga aacttgagaa
    12241 aatatcctca aaaccaccga agccacctgc cccattgaaa ccgccgaaac caccagctcc
    12301 accaaaacca ccattggcgc ctgcagcacc atactggtca taggcagcac gtttttggtc
    12361 gtcactcaaa gtctcatagg cttcttgaac ttccttgtac ttgtcctcag caccaggctc
    12421 cttgttgata tctgggtgat attttttgga aagcttacga taagcctttt tgatttcgtc
    12481 tgccgaagcg tttttggata cccccagacg atcataaaat tcagtattgt tcataattca
    12541 agatacaaag ggcgtcaaac ggacacagcc atctaggagt tttgacgacg gacgcttacg
    12601 tcctagaaaa aactatcttt ttggcacagt ccgtagcccg tgttcagtta cgaacaccct
    12661 tattcctttc tattgatgtt ctgaatcgaa ccaccattta ggtcgggttc aattcctttc
    12721 ttttatattt agtaagaaat tctgaaaccc gtgttcagtt acgaacactc tttgttcctt
    12781 tctattgatg agacaaaacc caaacaaatg agattccgtc cctttttatc gaaaaacaga
    12841 ggctgggttg caacctctgc aaataagtaa attaacttta ttttgatttt aaatatccta
    12901 taagtacaga aatatttata tatttttgtc tcttcttagt atttgcctat ataattccaa
    12961 ttgatcataa gaaatacaac tactgtccaa atcataccaa ttatgaacgg tattacaaat
    13021 ttatatgttt gtatttctaa tgatatcata gctgatacca tcgccgatac aaatgcagac
    13081 aacaatactg aggtaacaaa attccttacc gcttcatcat ttttgacaca actatcatgc
    13141 tcagctaata taatttctct atatctagct aaatattcta ttggaatatt atcaataaaa
    13201 tcttttgatg ttctatagtt atcagcttga ttacaaaaat cattataaac ttttcgtgta
    13261 ttttttagat actcttcatc caatacactc atcttacttt tccgtaaact ctccgtctac
    13321 gacgtcatcg cctgcgtttc ctgttgcttg tgcgccttct gctccttctt gagcttgttg
    13381 cgctgctgcg gcttgttcgt agagtttaac agcaagtcct tgagcttttt cgttcaatgc
    13441 ttcaagtttt gctttcatgt cgtccaagtt gttgtcttct tgagctttct taaggtcatc
    13501 aagggcagct tgggcagcgt cacgttctgc gtcgaagcct ttaccttcag tttccttgat
    13561 tgtcttttca gtcgcaaaga ttgcttggtc cacttcatta cgaaggtcta cttcttcttt
    13621 acgtttctta tcggattcag cgtttgcttc tgcatctttc atcatgcggt cgatttcttc
    13681 gtcagtcaaa cctgagttcg attggatgac aatagtttgt tctttttgag ttccaaggtc
    13741 tttggcctta acagacacga taccgttctt gtcgatgtca aatgttactt cgatttgagg
    13801 aattccacga ggtgcagctg ggatatcagt caattggaag cgtccaagag tcttgttatc
    13861 tgctgccatt gggcgttcac cttgaagaac gtggatatca acggctggtt ggttgtctgc
    13921 tgctgttgag aagacttgtg atttagatgt tgggattgta gtgttgcgat cgataagttt
    13981 tgtaaatact ccacccattg tttcgatacc aagtgacaat ggcgttacat caagaaggac
    14041 aacatccttg acatcaccag taatcacacc accttggata gccgcaccca tagcaactac
    14101 ttcatcaggg tttactgatt tgtttggttc tttaccagtt tcagctttaa cagcttcaac
    14161 aacggcaggg atacgagttg aaccaccaac aaggataact tcgtcgattt ctgacaagct
    14221 caaacctgca tctgaaaggg cttgacgaac tggaactttt gtacgttcaa caaggtcacg
    14281 agtcaaatca tcaaatttcg cacgagttaa agtcatttcc aagtgaagag gtccagcctc
    14341 acctgcagtg ataaatggca agctgatttg tgttgaagtt acaccagaaa ggtctttctt
    14401 cgctttttca gccgcatctt tcaaacgttg cattgccatc ttgtcagtag acaagtcgat
    14461 accgttttct ttcttgaatt ctgctaccaa gtggtcaatg attttttggt caaagtcgtc
    14521 accaccaagt ttgttgtccc ctgcagttga caatacgtcg aagacaccgt cacccaattc
    14581 aaggatagag acgtcgaatg taccaccacc aaggtcaaat accaagattt tttcttcttt
    14641 gtcagtcttg tccaaaccat aagcaagagc tgctgcagtt ggttcgttaa caatacgttc
    14701 tacttcaaga ccagcaattt taccagcgtc ttttgttgct tgacgttgag cgtcgttgaa
    14761 gtaagccgga actgtgataa cagctttggt tactttctca ccaaggtagt cttcagcgta
    14821 gcctttcaag tattgaagga tcatagctga gatttcttgt ggagtgtatt cttttccatt
    14881 tgcagaaact ttttcagaag ttcccatctt agatttgata gagataactg tatctgggtt
    14941 tgtaactgct tgacgttttg cagcatcacc aacgatgatt tctccgtttt tgaatgagac
    15001 tacagatgga gttgtgcggt ttccttctgg gtttgcgatg attttgcttt cagttccttc
    15061 aagaactgca actgctgagt ttgttgtacc taagtcaata ccgataattt tagacatgtg
    15121 tttttctcct tgttagttaa ttttctattt ttctttttga tactcttctt aagtggcgac
    15181 gccgtcagag cttgactttg tcaatctctt tgactaaact ttgagcctaa ggtctcaaaa
    15241 tttgcgcaaa tagcgccaca gcgaagagta tcgtctttgg ttcgcttcgc tcactattac
    15301 aaagccaaac atattttatc tttcttcata gtttattgtc gtttcggaca agttttctat
    15361 tatgtaaatt gcgacacgaa gagtcgaaat cgattttatt tcgacgacga gttagtaagg
    15421 aagctagaca aacgccatag cgactgccgt tttctgacga gttgctttag ctaccgtcag
    15481 aattgccgaa tcgaacacgc cctaagctct gagtaaaaaa gatagatttt cttgtgttca
    15541 tcgaacactt cgtcaatctc ctatttttac tgcgagcttt ttacgggctt tgtatcttag
    15601 ttatacacca ctaccattgc tgggcgtagg atgcggtcat ggagtttgta gcctttttga
    15661 aagacttggg cgatggtatc tactgggtgt tcatcgtctg ctgggagagt ttggatggcc
    15721 atatggtagt tatggtcaaa ttcgccatct gctgcgattt cttcaattcc ttcttctttc
    15781 aaagcgtgaa tcaagctttc ttgcaccatc cccaagccct tcttcacatc atctgtcaaa
    15841 ccttcaactg caagtgcacg ctcaaggttg tcaagagatg gtaagattgc ttttgccaag
    15901 tcctggctac gataacgttg caagttttga cgttcttcgt tggcacggcg ttggatattt
    15961 tgcatttctg catgagcgcg aagatatttg ttttcgaact catctgcacg ttcatttgcc
    16021 aagtccaact cagacttttc aggagttgtt tcttcagctg tttccacaac ttcctcttct
    16081 tgaacttctt ctacttcttc attttttata tcttgggcca tgttcgcctc ctttaatgat
    16141 ttcaatctta atgtacttca taatgattac tgctgaggta gcggtaaaaa tctgtcaact
    16201 tcatggtcaa aacacggttg accacattga cttgattgat tagctgttgg taatccagat
    16261 taactggacc gataatggct agaattccaa ctccccgata aggaatgagg aacttactac
    16321 taatcaccgc taggtcagct aaacaggaat cttgaccgtc tgcaacacga acattttgca
    16381 tctgatcctc acgcaacccc tcacgaatct ccaaggccac cttttgcggt tggtcaaaga
    16441 actgataggc tgctagattg gcaaaattca agagattgac cttgcccgcc atcacaatgt
    16501 tttcgttgaa catttcctta aagatgtgtt caaagagatc gatgacatta tccgttgttg
    16561 taaagtaacg ctggataatc tgcggaatct ccgtccgaat cttgtagtga atatctaaaa
    16621 cggtgtgacc gaggaaacgt tcctgaatga tgctcttcag tttcagcaaa tcctcctgca
    16681 agaagttcct tggaatcaga aactgactag taaccgttcg cgactcgtct agggtaaata
    16741 ccgccaaggc tgtatgttgc cccaaaacaa cgatatcaaa ggctgtcaaa cgttgcctgc
    16801 tcggctcaac atccagtgcc actaccgtac agccactcag gtctgttagc aagttagcag
    16861 cctcttgcag aatatcctcc aatttgaaga attcctgatc aaaggctttg acaatctcat
    16921 atacctcatt ttcagccagc cggtgaaaat ccagtgagtg tttcacatag tactgaaaac
    16981 cagcaacact tggcatccga ccgcttgaag tatgagcctt ctcaagcaac ccttgttttt
    17041 ctagttccgc catgtcatta cgaatggttg cactgctaga gttaatagac tcttgcaagg
    17101 cttttgatcc gacaggttcg tgcgttttgg taaagatgtc aataatcaga tttaaaatat
    17161 cctgctgacg ctctgtaacc atctcatcac tcctctctgt ctgatcaatt agcactcaat
    17221 gcatccaagt gctaacttat gattctatta tacaccctta aaagagaatg tcaagacaaa
    17281 aactcaaaaa attagcactc ttttatcaag agtgctaaaa atcaatctga agacctagaa
    17341 ccttgctatt catctactcg gtatttcctt tttagtctga aataaccatg aattagataa
    17401 gaaatcagaa aagcatataa aacaaaaata agaaagaaag acatagaaag ttcctctcta
    17461 aacaaactca tacaaacaac aagaccacct aagaaagtag ctgtacagga acgaagtcta
    17521 gatcgcataa cttcccattt gagcccctta ctcatataga cttgatcctc tggaagtaaa
    17581 tcagaagacg atttacgaag aatcgaacaa gaacctgctc ctatcaattc ccaacctcta
    17641 tctctaaatt cttgcagttc atgcttatat tttttaagaa atctagaatc atagatacgg
    17701 tagatgacat cgtctggttg acattggtca aaatagaaca aaccaaaacg actcgttcta
    17761 tacctccaac ctttcaaatg catctcatgt aaatattctt cttccttgtc caaatcaaga
    17821 atggtgaaaa tccgaaattc tactctgcta ttcattgtct taccccaaaa ttagaaaaca
    17881 tgcctggcgt tatttattag ataattcttt ccacttttga ctcaatctcc aaaaaatata
    17941 agaaatctga atcgcaaaaa ctatcaataa aacacaatct attatgaaaa tcaaaaatac
    18001 tcctcaactg aaagaactac ctccagtgac aaactttgag aaaaccggta gtagagctaa
    18061 aaagagaagc aaaataggaa gcatccgcat tgttaaaatc cgtttggcat aaaaaatctt
    18121 tatttaaact aaaatattat agcaaaattt acgccagttt ttgaacggct gatgtagata
    18181 ttttatactt tcaaaatgtt taaatgtgat tatttatttt tgaaaaatag ctcaccagcc
    18241 cgactgaaag tgcttataga atgataataa gtcgcctgcc gaaaacagcg gaaaatagcg
    18301 gtgttatgcg gagataatct gacgcgatgc gaaagtatat tgcatactta ttttcaacaa
    18361 tttagcagag tatttttata agtgtgatat aatagaagta taatttgttc tgatagttta
    18421 ttttatggag aagtagattt ttagaatgcg gagggttcaa tatggttgag tttataaagt
    18481 ctaagaaaga aatgagtgag gaggatatta aagcaaattt catcactcct gctattgtat
    18541 ccaaaggatg gaaaaatggt gagcatatcg cttacgaaga atacttcact gatggtcgaa
    18601 ttgaggttag aggagataag gctcgtcgta aagaaggaaa aaaatcagac tattcactgt
    18661 attaccaatt tggaactcga attgcaattg ttgaggcaaa ggataataaa cacagcgttc
    18721 gagcaggatt acaacaagct attgaatatg gagagatttt agatgttcca tttgtttatt
    18781 cttcgaatgg tgatggcttt attgaacacg accgtatcac gagagaagaa cgtgagctgg
    18841 agttagacga attccctact cgtgaagaat tattttctcg tatgacgaag gaaaaaggat
    18901 tgacgtacga aattacagaa gctatctcaa ctccatacta tacagacgcc ttctcaatga
    18961 aaacgccacg ctattatcag caaatagcta tcaaccgtac tattgaaaca gttgccagag
    19021 gacaaaaacg agtaatgttt gtgatggcaa caggaacagg gaaaacgttc atggcttttc
    19081 aaattattca tcgccttcga aaagctggtt tggctaaacg agttttattc ttagcagata
    19141 gaaacatctt agtagaccaa acgatggctg aagactttaa gccattcgaa aaggtaatga
    19201 cgaaaattac accaaaactt ttgactgctc ctgaaaaatt aaattctttc gaaatttatc
    19261 tagggcttta tcagcaacta actggtgaag atggaactga aacacattat caaaaatttg
    19321 acaaagactt ctttgattta atcgtaattg atgaagcgca ccgtggttca gctaaggaaa
    19381 acagtaactg gcgtaaggta attgattatt tcagttctgc gacacagatt gggatgaccg
    19441 ctactcctaa agaaaccaag aatgcttcca atacggaata ctttggtgag ccaatctata
    19501 cttatagttt aaaacaggga atcgaggatg gttttttggc tccatatcgt gttatgaggg
    19561 ttaatttaga tgtggatgtg gatggttatc gtccagaaac tggaaaagtt gatgctaacg
    19621 gacaattaat agaagatagg tactacggca ggaaagattt tgataaaacc attgtcattg
    19681 atgatagaac gcaaagagtt gccaagtttg tttctgatta tatgaagcaa aacaatgcac
    19741 gatttgataa aacaattgtt ttttgtgttg atattgacca tgccgagcga atgcgtgctg
    19801 cacttgtaaa agagaatcta gacttagtcc aagaagacta tcgttatgtc atgcaagtaa
    19861 ctggtgacaa cgctgaagga aaagctcaac tggataactt tatggatgtc aattctaatt
    19921 ttcccgctat tgtaacaacg tctaaattat taacgacagg agttaatgct aaaacatgtc
    19981 gtttgattgt tttagactct aatatccaat ccatgactga atttaaacaa attattggtc
    20041 gtggcacacg tctttatcct caaaagggga aagaattttt tacgattatt gattttcgaa
    20101 atgttaccaa tttgtttgct gaccctgatt ttgatggtga gccagtgaag gtgctggaaa
    20161 caggtgcgaa aacagtcagt ggttctacgc ccggtttcgt agatgaggaa ggtgacccag
    20221 tagaaaaata tatcgttaca gacaagcagg ttaccattct taattctact gttcaagtat
    20281 tggatgaaaa cgggaaactg attaccgaaa gcctgaccga ctacactcgg aagaatatct
    20341 taggtagcta cgccactttg aacgatttta tcacagtttg gcatacggcg gataaaaaga
    20401 agcttatctt agacgaactt tataaaaaag gagtttatct agatgctatt cgagagtcgg
    20461 agggaatatc agaacaagaa atcgatgatt ttgatttact cctaaaactt gcctatggtc
    20521 aaaaagaatt aaccaaaacg gaacgtatca ataagctcaa acaaagcgga tatttatata
    20581 aatatagtga ggaagcgcgt gctgttttgg aaattttact gaacaaatac atggataaag
    20641 gtattggaga actcgaaagc attgaaacat taaaacttcc agaatttcag atatatggtg
    20701 gaaccttcaa aatcatcaat acttattttg gagataaaaa acgatattta caagcaatta
    20761 aagaattgga gcaagagcta tttacagtag cttaatgaaa ggaaagtatg tcaattacat
    20821 catttgtaaa aagaattcaa gatatcactc gaaacgatgc tggtgttaat ggtgatgctc
    20881 aacgtattga gcaaatgtct tggttattat tcttaaaaat ttatgatagc cgtgaaatgg
    20941 tttgggaatt agaagaagac gagtatgagt caattatccc agaggaatta aaatggcgaa
    21001 attgggctca tgctcaaaat ggggaacggg tattgacagg cgatgaatta cttgattttg
    21061 tcaataacaa gttattcaaa gagttgaaag agcttgaaat aacttcaaat atgcctattc
    21121 gaaaaacgat tgttaaatca gcttttgaag atgcgaacaa ctatatgaaa aatggcgtct
    21181 tgttacgcca agtcatcaat gttattgatg aagttgattt caatagccct gaagatcgtc
    21241 attcgtttaa tgatatttac gaaaaaattc ttaaagatat tcaaaatgct gggaactcag
    21301 gagaatttta tacgccacgt gcagcgactg attttattgc cgaagttctt gacccaaaac
    21361 ttggagaatc aatggcagac cttgcttgcg gaacaggagg cttcttgact tcgactctga
    21421 accgtttaag tagtcaacgt aaaactagtg aagataccaa aaaatataat acagctgttt
    21481 ttggtattga aaagaaagca tttcctcatc ttttagcagt tacaaatctg tttcttcacg
    21541 aaattgatga ccctaaaatt gttcatggaa atactttgga gaaaaatgtt cgtgaatata
    21601 cggatgatga aaaatttgac attattatga tgaatccacc ttttggaggg tcagaattag
    21661 aaacaataaa aaataacttt ccagcagaat tacggagttc tgaaacagct gatttattta
    21721 tggctgtcat tatgtatcgt ttgaaagaaa atggtcgtgt tggagttatt ttacctgatg
    21781 gttttctatt tggtgaaggt gtaaaaactc gcttgaaaca aaaactggta gatgagttca
    21841 acttacatac gattattagg ttgcctcata gtgtctttgc accgtataca ggaatccata
    21901 cgaacattct tttctttgat aaaacaaaga aaacagaaga aacttggttt tatcgtttag
    21961 atatgccaga tggttataaa aatttctcga aaactaagcc gatgaagtca gaacacttca
    22021 atcctgttcg tgactggtgg gaaaatcgtg aagagatttt ggaaggtaag ttctacaaat
    22081 ctaaatcatt tacacctagt gaattggctg agttgaatta taatttagac cagtgtggct
    22141 ttccaaaaga ggaagaggaa atcttaaatc cctttgagtt gattcagaat tatcaagcgg
    22201 aaagagcaac tttaaatcat aagattgata atgtattagc tgatattttg cagttgttgg
    22261 aggacaaata atgacaccag aacaacttaa agcaagtatt ctccaaagag cgatggaagg
    22321 gaaattagtg ccgcaaaatc ccaatgacga acctgcaagt gaattattaa agagaattaa
    22381 agctgaaaaa gaaaaactta tcagtgaagg aaaaatcaaa cgagataaaa aggaaactga
    22441 gatatttcgt ggtgatgatg ggaaacatta tgggaagttt gctgatggaa gtactcaaga
    22501 aattgatgtt ccttatgata ttcctgatac ttgggagtgg gtgaggtttt ctacattggt
    22561 tgaaattgtc agaggtggct ctccacgacc aatcaaagat tatcttactt ctgaagtaga
    22621 tggaataaat tggataaaaa taggtgatac tgaaaagggt gaaaagtata taaataatgt
    22681 taaagaaaaa atcaaaaaat cagggcttaa caaaactaga tttgtaaaaa aaggtacatt
    22741 tttgttaact aattctatga gttttggtag accttatatt ttgaatgttg atggtgcaat
    22801 acacgatgga tggttggcta tttcgaacta tgaaaactca ttaaataaag attacctatt
    22861 ctatattctt tcatcaaatg tagtttattc tcaatttcta tctctaatta gtggagctgt
    22921 tgtgaaaaac ttgaatagtg ataaagttgc ttctattctt atccctctcc ctccgctagc
    22981 tgaacaacaa cgcataatag aagcaatcga atcagcttta gaaaaagtag atgaatatgc
    23041 tgaaagttat aatagactag aacagctaga taaagaattt ccagataaac taaaaaaatc
    23101 tattcttcaa tatgctatgc aaggaaaatt agttgaacaa gacccaaatg atgaatcagt
    23161 cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa ctctttgaag aaggcaagat
    23221 taaaaagaaa gatttggaca tttctattgt ttcccaagga gatgataact cttattatga
    23281 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
    23341 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
    23401 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
    23461 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
    23521 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
    23581 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag ttattaattg
    23641 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
    23701 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
    23761 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
    23821 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
    23881 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
    23941 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
    24001 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
    24061 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
    24121 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
    24181 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
    24241 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
    24301 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
    24361 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
    24421 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
    24481 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
    24541 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
    24601 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
    24661 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
    24721 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
    24781 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
    24841 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
    24901 tctgttggat aatgattact acattgatac acaattcatc tcctctgaac aagtttattt
    24961 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
    25021 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
    25081 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtctt ctccgttatt
    25141 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
    25201 aactacactg agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
    25261 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tgaaaatgat tcttttcatc
    25321 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
    25381 cctttgatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
    25441 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
    25501 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
    25561 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
    25621 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
    25681 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
    25741 aggctagcaa atttaatata tctccatgct tctgggattt tatatatagg ataagaggtt
    25801 gtttcgtctt tgttc
//
LOCUS       DAVKHZ010000021        25210 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000021 DAVKHZ010000000
VERSION     DAVKHZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.021"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            30..1239
                     /gene="sstT"
                     /locus_tag="VWW99_000827"
                     /pseudo
     CDS             30..1239
                     /gene="sstT"
                     /locus_tag="VWW99_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1307..2260
                     /locus_tag="VWW99_000828"
     CDS             1307..2260
                     /locus_tag="VWW99_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9538702.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(2496..2576)
                     /locus_tag="VWW99_000829"
                     /pseudo
     CDS             complement(2496..2576)
                     /locus_tag="VWW99_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(2631..2855)
                     /locus_tag="VWW99_000830"
     CDS             complement(2631..2855)
                     /locus_tag="VWW99_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU9538703.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFILLLTFMVITYK"
     gene            complement(2860..3039)
                     /gene="asp4"
                     /locus_tag="VWW99_000831"
     CDS             complement(2860..3039)
                     /gene="asp4"
                     /locus_tag="VWW99_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU9538704.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(3041..4378)
                     /gene="gtfB"
                     /locus_tag="VWW99_000832"
     CDS             complement(3041..4378)
                     /gene="gtfB"
                     /locus_tag="VWW99_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU9538705.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QERLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(4371..5882)
                     /gene="gtfA"
                     /locus_tag="VWW99_000833"
     CDS             complement(4371..5882)
                     /gene="gtfA"
                     /locus_tag="VWW99_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU9538706.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(5894..8266)
                     /gene="secA2"
                     /locus_tag="VWW99_000834"
     CDS             complement(5894..8266)
                     /gene="secA2"
                     /locus_tag="VWW99_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU9538707.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEGPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKEYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(8259..8699)
                     /gene="asp3"
                     /locus_tag="VWW99_000835"
     CDS             complement(8259..8699)
                     /gene="asp3"
                     /locus_tag="VWW99_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU9538708.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGSTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(8696..10231)
                     /gene="asp2"
                     /locus_tag="VWW99_000836"
     CDS             complement(8696..10231)
                     /gene="asp2"
                     /locus_tag="VWW99_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU9538709.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGVKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(10215..11795)
                     /gene="asp1"
                     /locus_tag="VWW99_000837"
     CDS             complement(10215..11795)
                     /gene="asp1"
                     /locus_tag="VWW99_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU9538710.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRIQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(11805..13022)
                     /gene="secY2"
                     /locus_tag="VWW99_000838"
     CDS             complement(11805..13022)
                     /gene="secY2"
                     /locus_tag="VWW99_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU9538711.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRIILPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     KMYLTLLIAVIQSLAVSLSLPVQSSYSVILVVLMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSL
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(13079..13984)
                     /locus_tag="VWW99_000839"
     CDS             complement(13079..13984)
                     /locus_tag="VWW99_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9538712.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDASTD
                     NSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIE
                     TLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFN
                     SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRS
                     GSIMRTEFDLSWARYIVEVFSKKISDCVLAGLDISVLRIRFVNLLKDYKQTLEYHQLT
                     DTEEYKDICFRLKLFFDAEQRNGKS"
     gene            complement(13984..15180)
                     /locus_tag="VWW99_000840"
     CDS             complement(13984..15180)
                     /locus_tag="VWW99_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9538713.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVHSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(15189..16409)
                     /locus_tag="VWW99_000841"
     CDS             complement(15189..16409)
                     /locus_tag="VWW99_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU9538714.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(16440..18884)
                     /locus_tag="VWW99_000842"
     CDS             complement(16440..18884)
                     /locus_tag="VWW99_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU9538715.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSIYLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTVMGPML
                     QELAKKYKNVKLYPEVKIDQFEKLKSQMDVYLDINLHNSTSEVVKEISSLNKPMLAFY
                     TSQNGNHGQHLYSSEHPERLLQAFHALIKGETLGEPEKPIKVIGIDQTLDYVIKNKSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHVDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(18894..19904)
                     /locus_tag="VWW99_000843"
     CDS             complement(18894..19904)
                     /locus_tag="VWW99_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9538716.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIS
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSNARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFMADSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(19933..20808)
                     /locus_tag="VWW99_000844"
     CDS             complement(19933..20808)
                     /locus_tag="VWW99_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9538717.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVVAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(20848..21726)
                     /locus_tag="VWW99_000845"
     CDS             complement(20848..21726)
                     /locus_tag="VWW99_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU9538718.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLFSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILNDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKRRD"
     gene            complement(21723..23807)
                     /locus_tag="VWW99_000846"
     CDS             complement(21723..23807)
                     /locus_tag="VWW99_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9538719.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIITQTYKNIEIVVVNDGSTD
                     ASGEICKEFAEMDHRITYIEQENAGLSAARNTGLNNMSGNYVTFVDSDDWIELDYVET
                     LYKKITEYQADIAVGNYYSFNESEGMFYFHISGDSYYEKVYDNVSIFENLYETQEMKS
                     FALISACGKLYKARLFEQLRFDMGKLGEDGYLNQKVYLLSEKVIYLNKGLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     AMYKEFEMKKRLLNQLTVQESSEKKAIVLAANYAYVDQVLTTIKSICYHNRSIRFYLI
                     HSDFPNEWIKQLNKRLEKCDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFVQEDK
                     ALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEIINQFARLGKTILSFENTKTYEAGQEVYAVDQV
                     QAMIEKLREISK"
     gene            complement(23868..25130)
                     /locus_tag="VWW99_000847"
                     /pseudo
     CDS             complement(23868..25130)
                     /locus_tag="VWW99_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT         7755 a         5470 c         4154 g         7831 t
ORIGIN      
        1 cataatcact acacaaaaag gagattatta tgaaaagaat cattcatgct tggaataagg
       61 caagccttgt caagcgtatc ttgattggta tgctttatgg ggaatcctag gactgaccct
      121 tcctaatctc tcagggattg ggctactcgg ggatttattc gttggaggtc taaaagctgt
      181 tgctcctatc ctagtctttg ccctcgttgc caatgccctt tcccaacatc aaaagggaca
      241 agatagcaat atgaaaactg ttgttttctt gtatatccta tgatagggac tttcgccgct
      301 gctcttgtag ctgtactagc aagtttcatc gtccctattg aaattaccct aaatagtgcc
      361 aatactgaaa ttgcaccacc agatgggatt gggcaggtcc tcagcaacct cttgctcaaa
      421 ctggttgaca acccagtcaa cgccctgttt actgctaact atattggaat cttatcttgg
      481 gcagtcattt ttggaatcgc tatgagagaa gccagtaaaa atagtaaaga attgctaaaa
      541 actatcgctg acgtgacttc taaaattgtc gaatggatca tcaatctggc tccatttgga
      601 atccttggtc ttgtttttaa aaccatttct gacaagggag tcggaagcct tgccaactat
      661 ggtattttat tggttccatt agtaacgact atgctttttg ttgcccctgt ggtcaaccct
      721 ttgattgcct tcttctttat gagacgcaat ccttaccctc tagtttggaa ctgcctccgt
      781 gtcagcggtg tgacagcctt tttcactcgt agttctgcga ctaacattcc tgtcaacatg
      841 aaactctgcc atgaccttgg actcaaccca gatacctatt ctgtttctat cccactcggt
      901 tctactatca atatggctgg agtagcgatt accattaacc ttttgaccct tgttacagtt
      961 aacactcttg gaattcctgt tgactttgcc acagcctttg tcctcagtgt ggtagcagct
     1021 atctcagcct gtggtgcttc aggtattgcc ggaggttccc tccttcttat cccagttgct
     1081 tgtagccttt tcggtatttc taacgatatt gccatacaag ttgttggggt tggttttgtg
     1141 attggtgtca tccaagactc atgtgaaaca gcccttaact cttctacaga tgtcctcttt
     1201 accgccgttg ccgaatacgc agcaacccgt aaaaaataac tcatcaaggc aagcctgctc
     1261 atgtcttgtc ttttacgctt ttattctaac ttattaggaa attcttatgt ctattagcca
     1321 acgtacgacc aagctcatct tagctacctg tcttgcctgc ctgcttgctt attttctcaa
     1381 tctttcgtca gcagtttcgg ctggaattat cgctctcttg agcctatctg atacgcgtag
     1441 aagtacttta aaactggctc gcaatcgtct tttttctatg cttctagctc tggctatcgg
     1501 tgttctagct tttcacttga gcggatttca tatctggagt ctcggcctct atctggcctt
     1561 ctacgttcct ttagcctaca agatgggctg ggaaattggc atcacaccaa gcactgtttt
     1621 ggttagccat ctcttggttc aagagtcaac ctctccagac cttctagtca atgaattcct
     1681 tctctttgct attggtacag gatttgcctt gcttgttaat ctctatatgc cttcacgaga
     1741 agaggaaatc cagcactacc acacgctggt ggaagaaaag ttaaaagata tcctccagcg
     1801 cttcaaatac tatttatcca gaggagacgg acgcaaccga gcacagctgg tagcagaatt
     1861 agacacgctt ttgaaagaag ccctcagact ggtctatttg gatcactctg accacctctt
     1921 tcaccagaca gactaccata tccactactt tgagatgaga cagcgacaaa gtcgtatcct
     1981 gagaaacatg gcccaacaga ttaacacttg tcaccttgcc gccagtgaaa gcctgatctt
     2041 agcgcaactc ttttcaaaaa ttgcaggtca actgagccag accaatcctg cttctgattt
     2101 gctagatgaa attgaacgtt atctggaagt cttccggaac cgcagtctgc ccaagacaag
     2161 agaagaattt gaaacccgcg ccacccttct tcaactccta cgtgaagcca aaaccttcat
     2221 ccaagtaaaa gttgattttt accaaaaata tagacagtaa tactcttcaa accacgtcag
     2281 cgtcgccttg ctgtatatat gttactgact tcgtcagttc tatccacaac ctcaaaacgg
     2341 tgttttgagc tgacttcgtc agttctatcc acaacctcaa aacggtgttt tgagctgact
     2401 tcgtcagttc tatccacaac ctcaaaacgg tgttttgagc tgacttcgtc agttatatcc
     2461 acaacctcaa aacggtgttt tgagctgact tcgtcagtta tatctacaac ctcaaaacgg
     2521 tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa
     2581 aaagaaaact tcagaccaat ccacaaaagg tgaatatctg aagttttctt ttatttataa
     2641 gtaatcacca taaaggttaa tagtagaata aataaagcca aactcaccaa taaagtgagc
     2701 accttgacca aggtgttgct ctgccagtag cttggtttac caatattact agtggcatcc
     2761 atggaaaata gttgattttg acggggttgt atagttacca atacaatcaa agcaagagat
     2821 aggataatag ctaaaacaat tagtatttca atcatagatc tacctcaaaa ttcccagcaa
     2881 agtaaagagc aaaccaatca gaatcattaa gcccagtaaa agtgaaaaag ttttactaat
     2941 cttctcccct cgggtttctt tttccttctt gatgggtttt tgtttcaact cttccatgcg
     3001 gccttcaacg tctttgtaaa ataaatcttc ctctgacatg ttagcctcct aaaacagttt
     3061 gcatggtttc ctgatatctc accaagtcaa cataatttgc atgttggcct tgtttgccaa
     3121 gtgcctgacg catttgatca acatctgaaa gggccaattt aatggtctca accaaagcag
     3181 caacttcact actttcaaat agatggtctg gagcgatata aagtctattg tgcaccgtct
     3241 gattaaagcc aagaatcaag agattgtgct caaaggcctg acgcactgcc tgtagcaact
     3301 cattactgtg gtttatatcc aagtaaatat ccgacagttg atacagctcc tgaatcttct
     3361 gtggactagc gttctggtaa aggaccacat taggatagcg aagcatgtct aagagcttag
     3421 aagacatctc tgtcaccgct gcaatacgga aagtgacatc aggcaaggct cctgcgattg
     3481 cttctacttg ctcaatctga tctgaattgg tcaagattaa ggcatctcgt cttaggaaat
     3541 tatcacgttt gaactggtaa tgataaccca agtgcacaaa ctgatcatgg tatttctcgt
     3601 cagttaactc taaagcgcgc tcataagtcg ccttatttgg aatgatgatc ttcttagtac
     3661 gcacattatc actttccaaa atcaactgca tattacctgg aatggcatca tagagacgtt
     3721 cctgccatac caagacatcc gagccagatt tatctggatg atggaaggaa accaagaaag
     3781 gagtcgctag agtattaaag ataagctgac tggtatctat ttccaaatct tgcaaaaaga
     3841 aggtgataaa ttcaacttta tttgcaaagt aacgcatgga ctgaccaggc aaagtcaata
     3901 agatatcacc cgtcacatgg ttttccagta aaacttgttg gccattgaca tcttggtaaa
     3961 ctgtcataat cggctcgcta tctgcgctat aagtggtttt agcaaagcaa gcaccaaagc
     4021 gattgtagtg gtcaacctga cgtactcgac cttctaggtc tttccagtct acctgtttaa
     4081 ccaagcgagc ctgcattcca tcaacataat gaatgacagc cctctcctgc gtcacatctt
     4141 caatacaagc agactgatta tctcctaaaa tttcccaaaa atctgaaacg ggaacttggt
     4201 taaaatagag aggttttcca tcctcgtaac ctagataata ggtaaaagga gaaagcagac
     4261 catcaggcag aaaaccatct gcatcgatga ccactccaag ttgagaaaga ccagtagcga
     4321 ctagactttc atgtaaatct cgactttctt gactgtaact atcataaagt tcaatcatgg
     4381 agcacctcct ctactgtttt cttccacttt tctaaaattt ctttggtcaa gaagccttct
     4441 gcaatttggt aagaataggc acgcatagct tccaaacgat tttcttgata caattgacaa
     4501 atcttagcgg cataagcttg cttgatttgg tcttctacat ggtcagatga acttggaatc
     4561 aaataaccat tttgcccatc ctctataaag gtctgattac cataaggcac atcaaaacca
     4621 attagaggta gacctgaacc aatagcttcc atcaaggtca gaccaaatcc ttcgctggta
     4681 gaagccgtta agtagacctc atactggcta taaatctgcg aaagttccgc atgccccttg
     4741 agttggatat agtcctctgc ctgatgattt gcaataatgt ctctaagcag agaatcttct
     4801 ccaccactac catagatatc aaaggttagt tccggtaact ccttatgagc ttcaatcaca
     4861 gctttcacaa gccaatcaat gtgcttttct ttggcaagac gtgaagccgt aatcaatgaa
     4921 aatggtttgc gcccttgact tgaatctgtc aaggaatcaa tactgcctac aggaatggta
     4981 acaatctttg gctgatgctg agtatatttg gcaaattgct cttgtagaac ttcattttgt
     5041 ctatcagtag acacgataaa gaagtcaacc ttatctgcat tggtaaactg atagtcataa
     5101 tagttattcc aaaggatgta gtcctcattt gtagcatttt cactataatg ctccgcatga
     5161 acaactaccg ctaaatgtgc tgtctgtgct tcctcaaaca caacctgacc aatgcctgtc
     5221 tccctatcga gaatgaccaa atcagactta ttcaaattca aagatttcat aaaggcacgc
     5281 acaaaagctt gctttccata gaaaatctta tccttgaaat gataaacttc ttccttccct
     5341 tgattcatca agatatcata gactggagtc ccgtcttcat tataaaaagt tcgttggtat
     5401 aagactgcaa cattgtcctt gggagcaaaa tactcgctac aataacgcgt ataagaaaag
     5461 taatccttcc gaatcaggtt tcccttaaaa acatactcgg catgttgaac caagtccttg
     5521 ttctcatcaa ccaaataaca ggttacaaac ttatcttggt caaaaaagaa tacacgtaaa
     5581 accttgccat ttttttctct gtgactttct tcaccaccaa agtaagccaa gacatcatcc
     5641 actgtcacgc tagtaggtgc aattttgata tctgtgaaat gattataaag ccagataacc
     5701 tgattatcat caaaaccaat attggctgtt aagtgctgaa tattatcggc taaaatcata
     5761 tctgtaaaga taaacttaga ggacagattt aatttccgaa aaacaccagc acgataggct
     5821 tgagcgtatt caacaccgct actagcccaa ccaattccta aatttatatt gtaaattgtc
     5881 atattttctc ctcttatgga aaatgagtca cgatttcacc ttttggagtg acctcaacca
     5941 gccccatcag gagattacgc accatatccg cacggatttg ttctttcata gcttcaaagc
     6001 ccgcgtaggc ttcttgatag tactctacga ttggattttt ctgactagca gattgaccac
     6061 cgatagccat ggatagctgt tgtagatagt ctacctgctc tacccagttg tcatcaatgg
     6121 ctttaagcag tgaaagtcgt aaaaactgtt catataagtc atgttgatta agtaattctt
     6181 tcttttcaga aagttcttta tcaatcacct gcttcataaa gctacgaact gcagttttgt
     6241 cagttacatc tatataatct ggaacctctt taacatgaaa actaatattg gtcacaataa
     6301 agtgaaacaa taattcacga ctagcatagt gatcagccgc tacctcttct gtatatctct
     6361 caatgatatc cacaacaaca tcctctaagt cacgagaacc atctattaga cgattacgct
     6421 ccttgtaaat catatcccgt tgaatattca tactttcagc atattctaga gtctgacgac
     6481 gtgccgaacg gccagcacta tcactagcat gttgagcctt ttcgactagt ttccggtatt
     6541 tacgaccttt caatacttcc ggttgagtca tatcttgaac ctgatagtct ttgtactttt
     6601 tatgcaccca agatggacca aatttcttga taacatcatc ctctaaggat acaaaaaatt
     6661 tactcatacc aggatctccc tgacgaccag aacggccacg aatttgtagg tcgatccgct
     6721 gactttccat ccgctcagtc ccaataacaa tcaagccccc aagctctgcg actcctttac
     6781 caagcttgat atccgtacca cgtcctgcca tagaggtagc cactgtcaca gcccccatct
     6841 gacctgactc ggagataatc tgagcctcac gcgccgcatt attagcattt aggacattat
     6901 gggcaatccc ttcacgaaac aagagagacg aataaagttg agacatttca actgagccta
     6961 caaaaacgag taaaggattt cccttagcat ggtattcctt gatgtactcc aaggatgcat
     7021 acactttttc aggtaaagtg atatatagat tatctggata gtcaatccgt tgtctcggac
     7081 gattggttgg aatgcgtact acagacatat tgtaagtttc aataaactct ttttccgcga
     7141 ccttacctgt ccctgtcata ccagatatct tattaaacat cttaaaaaga ctctgatagg
     7201 tgatcgaggc catagcccgc gtctcaggag ataatttgac atgttccttg gcttcaatag
     7261 cctgatggag acctccttga agtttagtca tttccattag acgccctgtt cccttatcaa
     7321 ccagtaccat ctcatttcca cgaatgatat agtccttatc tttagtaaag agcttatgag
     7381 ctcgaatcgc ataaaccaaa tgacgagcaa aagacgcatg ctcttccttg tataaattat
     7441 caatccctag gaaattctca gcagacttgg cccccttagt agtgagccaa acctcctctt
     7501 tctcctcttt aaagatataa tcctctcctt cgaccaaggt tgttacaagt gtatcaatga
     7561 tcgcatagta attagactga acacgaggag aacccgcaat aatcagagga gtttgtgcac
     7621 tatcaagcaa gatatcatca atttcatcaa taatcacata gttaaacggt cgtaaaaact
     7681 taccttcttc attcgaggct aggttatcat ttagataatc aaaacctaaa ttactattgg
     7741 ttgtgtagat gatatccgaa gcatagataa gcttcttttc ttcagctttc atttccttct
     7801 ttggaccttc cgtaaatggt acaccaatgg tcaatcctag aaaacgataa acttggccca
     7861 tttcctcggc atcacgcttt gataaatact cattaggagt cacaaccatc actccttctc
     7921 ctgaaaaagc gttcaaatag acaggcatgg tagctgtcaa ggtcttacct tcccccgtat
     7981 tcatctcagc aacatttcca tagtgcatga caatagctcc catgacttga acatcatagg
     8041 ggaacatccc taaaatccgc ttatctgctt cacgcaccac agcaaaagct tcaaccaaaa
     8101 tatcgtctag actttctccc tcggaaagac gctgacgaaa ctctactgtt ttagctacta
     8161 attcttgatc cgaaagagag gacatcttgc ctttcagggc attaatctgc tttaaaatct
     8221 ttttcacttt cctaagctgg aaatcttgac ctaaacgtct aaacacttct gatctccttg
     8281 atagaaaaat gataaaaagt taaggactca aatccagcac ttaaaagaga aacttgataa
     8341 gtataactat cttctggata agtaaatatg aaatctttcg ctttttctac ctttttttct
     8401 aaaatatcac catagcggtc aaaaaaagtt atcttggtat aaactccatt ttcaggctga
     8461 caatcaaaaa ccagtgatag ttgatactcc tgctttcttt ttaataaggg aagctgaggt
     8521 tgggttcgtg cagcctggta attgacacta gaaaaccaag tctttagaat ctcaccagat
     8581 ggcaatagag gattatacag acgaacactt ttgtcagggt aataagatac agttgatcca
     8641 tacatataag tcccaccaac ttctccccaa tgaatagact gtctctgtgt aataatcatc
     8701 ttttctccct tccaaaacca gtctctaaga ccattctata aaagtgcaaa aaccaagaaa
     8761 tattggtatc tgtatcatca ttgtgtcgtc cagcagttcc tttagataaa atcttcacac
     8821 ctgtattaca cagataagac actaattgtt cataggcttg tggatccatt tcttcatctt
     8881 tcatatagga taaaccaaag gttgtctttg aaaaatttgc ttgtttaaaa attttccaaa
     8941 aacgctgatc caactccgtc atatcttgag aacttgtccc acctgtatga tgaattaaac
     9001 aatcaaaagc taagttagag acacccggtg cgtccaaacg tccacgacta gctatagttc
     9061 ctaaattagc caagggctta ccaactatga tggcatgggg ttcaaaagaa gcaccataat
     9121 agagagccgg aaacgttccc atagaaagac ctgataaaat caaatcctta tgatcaaagc
     9181 ctaaataatc aagatagtga gtgatcgtat cctttacttt tccctctagc tcatccgttc
     9241 cgagataaaa agctcccccc tctaaacgtg gatcagaaaa taaaatgaag ggacatccaa
     9301 gagttttcat cataaagtaa ccctcgaatc cttctgcagg tcgataacct gcaaaataga
     9361 cagtcaaagg cggtttaaaa tcacctggat ggaagaaata gtttatttca tcacgcttgg
     9421 aatcatgtag gatatttcca ccaagtacaa acttaccaaa ttgttttcga ctccatcgtt
     9481 ggtgaagatt acctagtttg atagttccac gacctcttgc ttcaacagat atcgagagaa
     9541 agtagtctcc gtctcgacta tccataaaaa gtgcttgctc caagtctttt tcagatacta
     9601 ttacttcttc aaagaaatca tcaacagacc cactccacat tttacggatt actaaacgaa
     9661 agtcacaatt gccttccttc tcatattcaa gccacaactc aatagggagt gttttttgca
     9721 ccataatatt ataagcccaa taggcaagct gggcaaaatc ttgcccaaac tcaccttcca
     9781 aactgacata atccaatcct tgataagaaa tagctcctgt aaaatttgga ttgacttgta
     9841 tcgtcggagg aaagagttta tcaccatacc caccgccaaa aagagattta cttaaatcat
     9901 tgattagttg ttggggactt aaaaaatcaa ttgcttgtgc acatcgtttt tttagaaaat
     9961 ccacaacctc tctatcattt atttgtagat tctggttata gaacaaagta taaggttcaa
    10021 aatattttac ataggaaaat aagtctctgg aatactgacc atcttctatt aaaactgcat
    10081 gaaaacggtt aatatcctct ttttcaatta tttcacgaag ggtttttgat gagtttgggt
    10141 aaaaataata ccaactcata ttttcaggta tgtcatagtg attttcccaa ttggcaagtc
    10201 ctatctgtaa aatattaagt tcctttgaca tttttaacct cctctatcca tttctctaat
    10261 tttccaagaa attgttgtcc tgtgtgctcc ttaatcttat caattgaata tatcaaggac
    10321 tcattccact ccttcaacct atctgtgtaa taatgtgcag ccttagaaaa ttctgtaaca
    10381 tctgctaaca aataaccatt ttttaaatgt tcaacataga cggtttcaac taggttgatt
    10441 tgaggaattc ctgcactaat cccagctatc tgtgtgtaga gatgaggatg tctatttaaa
    10501 tccacaatca aacggacaaa ttctagtgtt tttatcaaat ctaactcatc attcaagttg
    10561 acaaactgta tacgtaagtc ctggtgttga ttttcttcca gaggattttc tgcatcacca
    10621 taatcaatcg cctttcccag attttcggat tgaatgtttt cttggatgaa agactcaaca
    10681 atcccttcaa cctcattcat ttgctcctga ctagcagcaa aggctccaaa aatcacctca
    10741 gtatccttat tttcagcgac aaaggacaag acttgaagca gagcttgatt atcaatcccc
    10801 tgctcaaaat ccagttgaaa ataaatgatg gattctttct tagtttggct tcgtcccaat
    10861 cgtaatcggg tgtcaaaaga tgataaatga taacattgat gcattcgttc aggatacaac
    10921 tcctgcaaca atcgtaaact atcctctcta tccaccaaaa tcaaatccga tttttcaaaa
    10981 gttacatcta aatccctaaa ggtatcttga ggattacgtc caatgaacag actcaacagt
    11041 ttaggattag tactaggtaa acgatctagt accaactggt catgatgaga atgagaagga
    11101 agcataaaaa tatcctgatc cttcacatac gtttgcagat aattctcaaa aaattctgcc
    11161 accagatctc ccatattttg ataggtaagc attttaaagc gataacccaa aattggattg
    11221 acttctacct gtcctccttc ttttaaacgc tctctaaatt gccaaactcc cttaggattc
    11281 aaatattctt gataggctgc ttgcccatct tcaaaaaaaa taatactcga tataaaccct
    11341 cgatcatcca tgatatggtg catatgtatt tgaccattcg gtccaaaata ctgtatatca
    11401 ctgatgaagc cttcaactcc atgttcaacc ttagcaaatt tcttcccatt tttttgaacg
    11461 ataatcgtaa agggactata aataaattca cagtcatcat cccactcaat atctctaatg
    11521 cttaaaactt gggtatggag attatgaaaa tcttgcataa catcaaaaac ggaataagta
    11581 tccgtttcta acacaccatg tctatataaa aaataacgta gatgaggctg gtaagctaat
    11641 actaatagac gagaatctat atcttgctct tgaaagagcc gaatctggtg aaaggtatca
    11701 tcaaactcta gacgaaaatg agaaaaatac catggagtga tatctgcatg ccatgttctt
    11761 tctgacccat accaagctgg aataaaataa tacataaaag tctcctaaat caaaggttta
    11821 taggtctcat ttagccttaa agccttgacc tcgtctctaa tcgtaaaaat catgcctcca
    11881 aacatcataa acaaacctgg aatcattgcc aatcgtaata acttttcatc aaacaaaaca
    11941 aaaagcatgg gaccacctgc cataaccaca ttaaagagac cacctatgac tgagaaacga
    12001 aggaccaatc gattaataaa tagactagta tccgcacctg gataaatacc ataaatgtat
    12061 tctccagatt ttttcatacg gtctgcaatc tcttctccat tcatcgtaac aaaagcaaaa
    12121 atgatactaa ataaaaataa gaccgaaata taaacataga cccacaaagg ctttccaatc
    12181 ataaattcct ttgataaagc cgctaaccct gaatgattag ggaaaataaa tcccaacaag
    12241 atgaacaaat aagctggtac actaagaaaa ctcatcacat acatataagg catcccacct
    12301 gcaggattca acatgatttc gagataagaa tagcgtttaa atcgattgtg taagccaatt
    12361 ttattaacag gaaccaaata gcgagctcga tacataaggg caagtaaata agaaaagaca
    12421 agggttaata aagcaagtaa cacaataatc cctgttggaa tgtgtactgt ctgaaatgtt
    12481 tccaaaacat cctgaggaat atttaaaacc atactggata ggaggattac aatagaacct
    12541 ccaatcccca tactcgcatt taaatctgac aaccaaacaa gaaaaaatgt tcctgctatc
    12601 agcaacaagg tattcattag aacaaccaaa attaccgagt aggaggattg tactggcaag
    12661 ctcaagctaa ctgccaagga ttgaatcaca gcaattagca aggtcagata catttttcga
    12721 cgatcttgaa tttcaatcgc tgtagaactt agccccaatt ttttagaata tgaaaacatc
    12781 tgccacaaaa tcatagctga catccaaggg gataatccaa cagaaaaaat tgacaaactt
    12841 cttagatttc cacctgttag ggcggctgag aaggctagat aggctgttga acctcctaaa
    12901 aaatctttag tatttaggtc aacaaaaggg agaataatac gacttcccaa tacatagata
    12961 aacagtagaa ataaggtaaa tagtcctttt tttactgcta ttgacgaata aatcttagtc
    13021 ataaaacact cccctcacct tcattgtttg tgatatggta aataacaatt cttttatttc
    13081 aacttttacc atttctttgt tctgcatcaa aaaacaactt taatctgaaa caaatatctt
    13141 tatattcctc agtatctgtt aattgatggt attctaaagt ttgcttataa tcttttaaaa
    13201 gattgacaaa tcgaatacgc agaacggaga tatccaaacc agccaagaca caatccgata
    13261 ttttcttaga aaacacttca acaatatatc ttgcccagga aagatcaaat tccgttctca
    13321 tgatgcttcc tgaacgtctt ctatacagat agagattatc gtttataaag acgattttag
    13381 aggctaaaag ataaaagcga tgcatagttg cttcatcatc aaagcggcga ccatttgaaa
    13441 agtgaacttc attgaataat tcttttttaa tcaacttaaa tgtcggcaat ataaaggccg
    13501 agctattgaa tttccaatct cctgcttgac ggttcataat ttcttgtata gctaattctt
    13561 cgacgcaaaa atcttgccct gttacataaa aataaaaatt cccgtcactt tcgtcataat
    13621 tatagtaatt ccctataaca acatctgcat ccttttcttg gacttgctga taaagagttt
    13681 caatcatatt ttcttttacg atatcatctg aatcaacaat cgtgatgtac tctcccttag
    13741 ctacactaat ccccatattt ttagcttgtg ctgccccccc cttattttct tgatgcaata
    13801 ctcgcacacg attatcttgc atcataaaag catcacaaat ttccccagaa ttatccgtag
    13861 aagcgtcatc aactaaaata atttctatat tttgataggt ttgcttcaga atatgctcga
    13921 cacactccac taaatatttt cccgtattgt atatcggtac cacaacacta attaattcac
    13981 tcattactct atctctatat tcttcattct atccactaaa tcatctggtc tctccacaga
    14041 aaaaataccg tcatagagtc catcatccat acttttacgt gtgatatcaa aagcgaagat
    14101 tttcttgcca ctttcttgtg cccttgtaac tacattaaaa acctctccac ctgtattaat
    14161 atctaaataa actatagaat cgtccaatag ccaatcaatt ctactatgta atacattttg
    14221 atatactgtt acattcgtat actgtgatag agaggtcagc tcctcagaac aatcacacgg
    14281 tgcagccaaa tgaaaatgcc aatcaggtaa ccgttgtact aaatactcta aatgttttat
    14341 atctgcactc catgtcacaa gcatcacttg ttttttagac tgattgcttc tttcaaaata
    14401 atttaaatcg gaacgttgaa aagcaatctc tgaccaatcc aaatctctat aaacccacca
    14461 taattcacgt agtctagata tactatatgt attccaaggt ttatcatgac tagcatagtg
    14521 aacaattgta ggtggattgt catctagacg ttcacattct tgagcaaggt gataaatatc
    14581 aacacccacc atataattat atgttttatc taaggctagc caattatcct caaaataaat
    14641 atttaaaata ctctgatccc caagatgaac aacttgattc tgctcggccg ctaattccaa
    14701 taaactattg acaatagaat gttctttcca ctttgcaaca tccattagta acataccagc
    14761 attaaatcca gattttcgtc cttcataggc atagacatca tcaacagcac caattgaata
    14821 tcctttgaga tctatctcaa acaaagtagc tagttcccca gttacaatga tatcggaatc
    14881 cagatacaat accctatcag attcaaccac ttctgtcgca aaaaatctaa agtaagaagc
    14941 ataatttata tgaggtcctg ttttatagct ttcaaaaagt tctttatcaa tgtgtacact
    15001 gcgaattgta gaatgcacaa cttccatttt tctccccaat aatttaaacc attcaggagc
    15061 tatatcactg ttgagaatat aaaaatcaac atctctattg tgatacaata cagactttat
    15121 agtcgtctct aaaggaataa gataggcatt atctgccgct aatactattg attttctcat
    15181 actgttctct acctcatgta agatctgatt gctgcaacca tttttttcgg aatggagctt
    15241 ggatagatac cttcatatga aatctcagaa agattttcag agcaagtatt gtcgaaagct
    15301 atcattggtt ttttgtactt aagcacaaac tcatatgcat cttctaattt tcgatcatga
    15361 ttcaaatcca aatacaaatc tgaagctatt actatttgtt ccaataacat tggtaaggaa
    15421 tttggatata cggtaacatt aggaaaacgc gttaaagcta gtagttcatt tgccatattt
    15481 gtataagcaa caatatgaaa atgaacttct ggcaattgct ccgccaaata atcgattttc
    15541 tcgacacacc aagaattcgt taaattaaca cattgcaact taaatatttg tgatttacta
    15601 ggatacttca cactctttga aaaccattca tttaaaataa cagaccaatc catcaaagag
    15661 tattcccacc aaacttctct tagacgtcca acagaaaatt gattccaagg cttatcctga
    15721 gaaatatagt gtaaaattag tggcagtggt tcgagcggaa tatcaaaaat gaattgatgt
    15781 ttaaaggctg ccgccccata atcatatcct atttgaaaat tatattgatc ttcaagggaa
    15841 ctatattgat ctttaaacaa catattcaaa attgactggt ctccttcttc cacattctca
    15901 tgttcttttt ctgttaagtc aatcaatttt tgtcgaatag tttcagatcc ccattttttg
    15961 ttgttaatca agagaacacc agcattgaag ccgactcctg ctccaaagca agaacgagct
    16021 gctgccaaat aattttcacc taagtctaat tcaaacaaat cggtcaaatc accagtcaca
    16081 atcaaatcac tatctagata gagaacttta tcttctgtta caaaatctgg aataaaatag
    16141 cgtgcaaatg tcatatgatt gatatgaggt aatttattag accaattcat ttgaaactgt
    16201 gagccaatta acttgcagtc aatcaagtcg ccccccatct cttgtaaata tattcttatt
    16261 tgactaaacc attcctgagg aatgtcctga tttagcagat aaattttcaa atgactattg
    16321 tgtctacaga gtgacttcat cgccgtttcg atttgtcgaa tataagcgta atcacctgca
    16381 aatactacag cgcgtttggt atttctcatt ttaaatcctt atcatttatt ttaaatagtc
    16441 tagtttaata ttcgtgcaac aatctgactg ttataggttt catcatcaat aaattcaata
    16501 tcttggtcga aattatgttc tgcagtatgt ttatgagaaa atttaacctt agttttagct
    16561 cccattttca tccattcata ctctgagtca acatggccta catccaaaac ttgatagccc
    16621 atctggcata gattataact cagaactttt gctgtaggac ccagcataca taaaatcagt
    16681 cttccttcag catgtttcat aatttcctct tgaatgttat cgacaacgga ataagcatta
    16741 tgagatggac aaataatcct tttcacagat ttgaccttat caaataaatc atttccaacc
    16801 ccagagcgag atgtaatccc ctctacaatc agaatatccc tattctccca aatactcttt
    16861 agcttttcaa attgtccttt tgccttactc ttatcttcat aatcaatgta tggacgcgag
    16921 ataaaggtag agccatacca tgaaccctta cacaactctt tataaaaatc catataaaca
    16981 ttcatatgtt ctttccagaa tggtgtagcc caccaagtaa agacaaaacg atcgtcaaaa
    17041 gcatcaggta aacaaaccac taatttctcg tcactctcaa gacccacaat atgttttaat
    17101 tggttagcta actctagatc atgattttga taaggtatag gcaaacccca catcaaattg
    17161 acttctccgt ctccaaaacg aactaatgag gacttatttt tgataacata atccagagtc
    17221 tgatcaattc cgataacctt gattggcttc tctggttctc ccaaagtctc tccttttatc
    17281 aatgcatgaa atgcttgaag aagcctttct ggatgctcac ttgaatataa atgttgacca
    17341 tgatttccat tttgtgaagt ataaaatgcc agcattggtt tattcaatga tgatatctcc
    17401 ttgacaacct cactagtaga attgtgtagg ttaatatcca aataaacatc catttgagat
    17461 ttgagttttt caaattgatc gattttcact tcaggatata gcttaacatt tttatatttt
    17521 tttgctagtt cttgtaacat tggtcccata actgtccaag cagcaacttg aaaaacaaca
    17581 tttggcaact gtccagccaa gtaatccaat tgttcgatat cctgagaagc cgtaaacgtt
    17641 aaacaagtta aacttggaag ttgtcgaaaa caggtaagat gtccgttttt cttaaaatct
    17701 ttaatcgtat caatcataaa ggaaactttc ccattttcga aaacgatttg tcctaactct
    17761 ccgtgctgag tcgattgaaa agctagtagc gttttttctt gttcttgcaa agattttaaa
    17821 aagttctcat ctgcactacc atgattgata tccaaatata gatttgttga ctttttcagc
    17881 ttatctaata caggaggaac aatttgtgga tgcaatctca cattattata tacagctaat
    17941 ttttttaatt tatctcccat atccgtccaa gctgcgatat gaaataccac ctcaggtaga
    18001 gctgtaacta gctcttctat tccttctaaa tcttgggaat tcgttaaggt taagcaagaa
    18061 aattccttat ctagaggcga tattagttca aattctccca tatggtgttg taaaatctga
    18121 ctccactcca aatcatggaa ttcccaccat aaatcacgat aacgattggc tgtcaaggta
    18181 gtccagggtt ttctgtaggt cgtaaaatga atcaccactg gtttatcatt aaaagccaga
    18241 tgttcctgcc aattgttata caaagccaca atatcatgcc ctacttgtaa attataagct
    18301 cgacctagct ctaaccaatc atcttgtaag acctgattaa aaatcgtttg atcaccatta
    18361 aaatgctcga aacggccttc ttcaacttcc ttcattgtaa caatgctctg ttcaattagt
    18421 cgttctttta atttctcttg acgccatttt ttattgttga ttaataaaac acctgtatta
    18481 aatgtaattc catctgtatc tttaactgct gcgagtgatt tttcttctaa ataaatacta
    18541 aataatttct ctaaagaagt atttacaatc aaatcactgt ctaaatataa aaccttatct
    18601 tcttggatat aatctgcaat aaaatatcta gcataggtaa tgctactaat gtgatcttgc
    18661 ttttcccaat cttgaaacac agtttgttca ggtagcttca catcgataat ctcactacct
    18721 aacatgcgag ctatttttcg tggctttcga aaccaatctg gcatgatatc ttgatttaaa
    18781 atataaatct taacatctcg attgtggtat agaatagatt ttatggttgt ttctaactgc
    18841 ctggtgtaat tacgatcacc agctaataca attgttttgt tcatttcact cccttaatca
    18901 attcccaagt gataaatcgc atctaccaat agctttttag tgaaatagcc ctcttttaac
    18961 aaatagctaa aggtcttgat acgattcgtc atttcttgat attcttgtag attcatttta
    19021 tcaactatcg catgtacttc ctcaaggcta tccgccataa aacctaagcc ctgatctact
    19081 ataaatttag cagttgataa gttgctcggt acaatgactg ggatgcccgc tgttagatag
    19141 gtactaacct tatgagatat atttaaagta tagtattgat tgctttctcc gtcattttga
    19201 tgcgttcccc agacaagacc aaagccacct tttgataatt ctagcaacag ttcctcatct
    19261 tttttccatc cttcaatgct aagatttcta gcattggaac tagcttctcc tttatttgaa
    19321 aacaccctca aaggcgtatc ttgagaccaa ttttgtaagt ctggaaaacg ctctaaactt
    19381 ccagcaaaaa aaagttcttt tttaaaagca ggggtgtata aggataaatc atgaggatga
    19441 tcccacatcc cttgaacaag aatcttctta gtcgtcaatc cttcttccat caggcgtgtt
    19501 ttcattctct ctgacggcac tatcaaaaca tctgatagat tatacatgta catataatct
    19561 ttcatgagat aatagttact atcaaacatg aggggaacaa catcatggat aaagcaaata
    19621 attttcacct gcatatcctt tagcttatca aacaagagac gatcaaattc aaagccattc
    19681 caggttggag actgaaaaac taggatatct ccaatagaga tactggccat aatgccatcc
    19741 agacgcttat tcatttcaga aggactatct gaagaaatat tgtaaaaata gatgccaacc
    19801 tctctaaatc ccagttgact ggctatcttt tgaacagcat tttgcgctaa gataaccgta
    19861 ctatcaccag ccatgccgta taaatttgtt aaatgtagtt tcatattatg ctatctcatt
    19921 ttttattact atctacggaa gtaaagacaa tttttccatc attcttctat aaatttctgt
    19981 gaactgcata ttttgttctt ctaattttgc tactactctt tgtaatctat ttttataaat
    20041 caaaatatga ttggtcaagt catagtctga acttgctaaa atagctatct tttcttccct
    20101 agccccaatt tcatcataca tacgcttttc actccatgta ttcgataaag tatcagataa
    20161 accaacacgg taccagtaaa cacaacgatt caaatagact atcctcgaag ctcttagaag
    20221 taccttccat gtccagtaag tatcctctgc aatttttcct ataggaaatg gtaaatcttg
    20281 tagtaactct ctcttgaata acttcaagac agctacagtc cagttcccat ttctgacttc
    20341 ttcgacacct tccctatcca tgattgcttt accttctatc acttccagag aatcatctgg
    20401 atccgtaaca taagtcatat acacatagcg tgtttcatca taagaattat aacgcccgat
    20461 actaatatct gcgttttcct ttttcaaagc accatataat cggtctaaag catcatgttc
    20521 caaccaatca tcagagtcta caaaagtaat gtacgccccc cccgaacatt caatacctag
    20581 gttacgagct gatgaaagac cgccgtttgc tttctcaaaa tatttgaaac gagaatcttt
    20641 ctctacaaat tcttcacata ttttggatga atgatctgga gagccatcat tgattaataa
    20701 acactcaaaa ttttgatacg tctgattctg aatgctatcc aaacacattc gcaaataatt
    20761 ctcaacgttg tagattggga ctacaatact aattagttca ttttccatat actaactttc
    20821 ctagacttga tttattctat ttccctctta atctcttctc tttacaaaaa gatttccatt
    20881 attataaact acactgtttg atggaatatc attgcgtatg agacaacctg ctccaataat
    20941 ggtattatcc ccaatcgtca ctccttttaa aatcgtgaca ttacttccta tccagcagtc
    21001 tctgccaact ctaatcggcg ctgtagtcca ctgccacttt tcaatctttt cagcagtata
    21061 aatatggtcg tgatcataaa aacgaacacc ctctcccatc atggtaccag ctccgatttc
    21121 aattctttcc atacagttaa aactacaaga gttgttgata aaaacaccat cattcaggat
    21181 taacttacca gaagagacat gaaaattttc aaaatttctg cagatgacat ttgctcctac
    21241 aatgaagtga gccttatcat cgattttaaa gacattccaa gtcccatctt ggatgatttt
    21301 ctcttgatga ttggtgccta ctctgacttc attcagatag tctctcactc tagaaaacaa
    21361 tttgtctggt gtacttgata gaaaaacttc ctgcccctga tttccatgga ctgtattgtc
    21421 aaaagcaaag acagattttc cagcctgtac aaacttggag acaatagcat ctacttcttg
    21481 aaagtgattg atatctagta aaaagtcaca ggtatcgact agaaagttta gctgttcttc
    21541 atttgtaatc gttcggagac ggacattata gttcgtttct aagctacgga ttttctcgga
    21601 aaattgaaca ggtgctgcaa tataaaaaca gatatttggc aaggattcaa ggagataatc
    21661 caactcttca atctgatcac taacagtcaa aatcagagca tctcctacta aatgattttt
    21721 cttcatttgc ttatttctct caatttctca atcattgctt gaacttggtc aacagcatat
    21781 acctcctgac ctgcttcata ggttttggta ttttcaaagg acaaaatagt ttttccaagt
    21841 cttgcaaatt gattgataat ttcctctgtt ttttcaccat gattaatatc taaaagtact
    21901 tgactggttt ctaccaattc attatcgaca tctaccaaat agtgaattcc gttaaatata
    21961 gtcacgtttg gataaattgt catctgagcc aatcgatcac taactattac tctagctgct
    22021 atcttaaact gaatatcagg caaggattga accaatgtct caatttgttc aatatggtct
    22081 gaggcagtat agattagaca agtgaaaggt tcctttattg gatagatgtg agatctttgt
    22141 aatggatgta aatgatggtt ttgtcccaat tctgtccatt caagcccatg atagtaccac
    22201 caaacttcac gataggtttg ggccgccaaa tctttccacg gtttccgatg agaaagatag
    22261 tgaataatag caggataatc ctgaccctca ggcaattgat aatcagcaaa ctgtttatga
    22321 atgacaatat gattataatc aaagtccaat tccaaccatt tatgttcaaa aagcatattc
    22381 aagatgctct gatctgcctg atccacctta tcatgccatt cattggttaa atcaattaat
    22441 ttttgggtca tattctcttt tttccaaaaa gcattgttta ccaagagaac accggcatta
    22501 aagatttctt gaccaaaata agctctgccc ccaaaatctc taacagcagc caaaggataa
    22561 tcttgtaagt ctgtagcaaa caagtcatcc agatttttcg ttacaactag atcacagtcc
    22621 aagtagaggg ccttgtcttc ttgcacgaaa tcagctatga aatagcgtaa aaagactgtg
    22681 taactaatat ccgatttata acatgaaatt tgctcagaag ttacccgaca attaataatt
    22741 tctgagtcac acttctctaa gcgcttattt aattgcttaa tccattcatt tggaaaatcg
    22801 ctatgaatca gataaaaacg aatcgaacga ttatgataac aaatagactt gattgtcgtt
    22861 aaaacttggt ctacataggc ataattcgcc gctaggacaa tggctttctt ttcacttgat
    22921 tcttgtaccg ttaactgatt taaaagtctt tttttcattt caaactcttt atacattgct
    22981 gtgtcagata aaccgctagc ttgaccgttg gcgagactga cttccaacat ctgacgataa
    23041 actgccaagt gtttctctag aggataaccc atattagcta gtagcgtaat acgttcagac
    23101 atagcatcaa ctaaggcgtg catccacttt tctgtccaaa ctcttgataa actacctttt
    23161 ctaatccgat aagcataaag acctttattt aaataaatta ccttttccga taataaatat
    23221 accttttgat tgaggtaacc atcttctcct aatttaccca tgtcaaagcg caactgctca
    23281 aacaatcttg ccttatagag tttaccacaa gcagatatca aagcaaaact cttcatttct
    23341 tgagtttcat acaagttctc aaagatggaa acattgtcat ataccttctc ataataggag
    23401 tctcctgata tatgaaagta gaacattcct tcactttcgt tgaaagaata gtaattccca
    23461 actgcaatat cagcttgata ttctgttatt tttttatata gggtttctac ataatctaac
    23521 tcaatccaat catctgaatc cacaaaggtc acataatttc cggacatatt attcagaccg
    23581 gtgtttcgtg cagctgacag cccagcattt tcttgttcta tataggtaat tcggtgatcc
    23641 atttctgcaa attctttaca aatttcacct gaagcatccg tagaaccatc attaacgaca
    23701 acaatctcaa tatttttata tgtttgagta ataatactat ctagacattt cctcagatag
    23761 ttttctacat tgtatactgg tacaatgacc gtaattttat catccactag caattttctc
    23821 cttgatttat gtgcgcgagt tagtttagcc taactttaca ggttgtctta ctcttcttca
    23881 tcacgtttac ggcgtttcgc aaccaatcca atacctgtaa cagctgctag aactccaagt
    23941 aacacagatc caattgacga ctcagtacct gtattaggca attctttttg ggatttacct
    24001 gtcgatccag aagcatttcc aacttgtgag ttcgaatggt ttgctgaatt tgaaactgat
    24061 gtacttgctg aagctgacgc actggttgat gctgaggctg aggcgctagt acttgctgaa
    24121 gccgaggcac ttgttgatgc tgaggctgag gcgctagtac ttgctgaagc tgatgcactt
    24181 gtcgatgctg aggccgaagc actggtactt gctgaagccg aagcactcgt tgaggctgag
    24241 gctgaagcac tcgttgatgc ggaagctgat gcacttgttg atgccgattc agacgctgag
    24301 atacttgctg aggctgaggc gcttgtactt gctgaagtcg acgcactggt tgaggccgat
    24361 tcagatgctg acgtacttgc acttgttgac gccgatgtgc ttgcacttgt cgatgctgac
    24421 gcactcgttg atgcggaagc tgaggcgctc gttgatgcgg atgctgacgc actggtgctt
    24481 gctgaagctg atgcactcgt tgatgcggat gctgacgcac tggttgatgc agattcagac
    24541 gctgagatac ttgctgaggc tgaggcactt gttgaggccg attcagacgc actcgttgat
    24601 gcggaagctg acgcactggt tgacgctgag gctgacgcac tcgttgatgc ggaagccgag
    24661 gcactggtgc ttgctgattc agatgcactt gtcgatgctg aagccgaggc acttgtcgac
    24721 gctgaggctg aggcactggt tgatgctgaa gctgacgcac ttgttgatgc tgaagctgat
    24781 gcacttgttg acgctgaggc tgaagcactt gttgaggctg aagctgaggc actggtgctt
    24841 gctgaagctg atgcactagt acttgctgaa gctgatgcac tggttgatgc tgaggctgaa
    24901 gcacttgtac ttgctgaggc tgaggcgctt gttgatgcgg atgctgacgc actggtactt
    24961 gctgaggctg aagcacttgt tgatgctgaa gctgaggcgc tagtacttgc tgaagccgag
    25021 gcgctggtgc ttgttgaagc cgaagcactg gtgcttgctg aagctgaagc gcttgtcgat
    25081 gctgaggctg aagcactggt tgaggccgat tcagacgctg aggtacttgc tacagatgac
    25141 ttggaaactt ctgtacctgt atttggcaat tgctgtcttg acttacctgc tgaagccgag
    25201 gcgcttgttg
//
LOCUS       DAVKHZ010000022        24464 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000022 DAVKHZ010000000
VERSION     DAVKHZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.022"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            9..89
                     /locus_tag="VWW99_000848"
     tRNA            9..89
                     /locus_tag="VWW99_000848"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="VWW99_000849"
     tRNA            94..164
                     /locus_tag="VWW99_000849"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="VWW99_000850"
     tRNA            179..251
                     /locus_tag="VWW99_000850"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            265..336
                     /locus_tag="VWW99_000851"
     tRNA            265..336
                     /locus_tag="VWW99_000851"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Gln,seq:ttg)
     gene            346..429
                     /locus_tag="VWW99_000852"
     tRNA            346..429
                     /locus_tag="VWW99_000852"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Leu,seq:caa)
     gene            536..1813
                     /locus_tag="VWW99_000853"
     CDS             536..1813
                     /locus_tag="VWW99_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9538720.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1889..3373
                     /locus_tag="VWW99_000854"
     CDS             1889..3373
                     /locus_tag="VWW99_000854"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9538721.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3434..4714
                     /locus_tag="VWW99_000855"
     CDS             3434..4714
                     /locus_tag="VWW99_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9538722.1"
                     /translation="MFKKNKDILNIALPAMGENFLQILMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4718..5338
                     /locus_tag="VWW99_000856"
     CDS             4718..5338
                     /locus_tag="VWW99_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9538723.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5504..6616
                     /locus_tag="VWW99_000857"
     CDS             5504..6616
                     /locus_tag="VWW99_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9538724.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6939..7364
                     /locus_tag="VWW99_000858"
     CDS             6939..7364
                     /locus_tag="VWW99_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9538725.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7375..7734
                     /locus_tag="VWW99_000859"
     CDS             7375..7734
                     /locus_tag="VWW99_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9538726.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7841..8485
                     /gene="pcp"
                     /locus_tag="VWW99_000860"
     CDS             7841..8485
                     /gene="pcp"
                     /locus_tag="VWW99_000860"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9538727.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGIFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(8513..9370)
                     /locus_tag="VWW99_000861"
     CDS             complement(8513..9370)
                     /locus_tag="VWW99_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9538728.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9500..10642
                     /gene="tgt"
                     /locus_tag="VWW99_000862"
     CDS             9500..10642
                     /gene="tgt"
                     /locus_tag="VWW99_000862"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9538729.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10740..12557
                     /locus_tag="VWW99_000863"
     CDS             10740..12557
                     /locus_tag="VWW99_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9538730.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12710..13861
                     /gene="nagA"
                     /locus_tag="VWW99_000864"
     CDS             12710..13861
                     /gene="nagA"
                     /locus_tag="VWW99_000864"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9538731.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14024..15082
                     /locus_tag="VWW99_000865"
     CDS             14024..15082
                     /locus_tag="VWW99_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9538732.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15233..15598
                     /locus_tag="VWW99_000866"
     CDS             15233..15598
                     /locus_tag="VWW99_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9538733.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15673..16614
                     /locus_tag="VWW99_000867"
     CDS             15673..16614
                     /locus_tag="VWW99_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9538734.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16562..17578
                     /locus_tag="VWW99_000868"
     CDS             16562..17578
                     /locus_tag="VWW99_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9538735.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17580..17906
                     /locus_tag="VWW99_000869"
     CDS             17580..17906
                     /locus_tag="VWW99_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU9538736.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            17875..18303
                     /locus_tag="VWW99_000870"
     CDS             17875..18303
                     /locus_tag="VWW99_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9538737.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18308..18568
                     /locus_tag="VWW99_000871"
     CDS             18308..18568
                     /locus_tag="VWW99_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538738.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18531..18992
                     /locus_tag="VWW99_000872"
     CDS             18531..18992
                     /locus_tag="VWW99_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9538739.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIHRVEKEKS"
     gene            18970..19383
                     /locus_tag="VWW99_000873"
     CDS             18970..19383
                     /locus_tag="VWW99_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9538740.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            19520..20114
                     /locus_tag="VWW99_000874"
                     /pseudo
     CDS             19520..20114
                     /locus_tag="VWW99_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20175..21128
                     /locus_tag="VWW99_000875"
     CDS             20175..21128
                     /locus_tag="VWW99_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9538741.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKENNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21179..22369
                     /locus_tag="VWW99_000876"
     CDS             21179..22369
                     /locus_tag="VWW99_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9538742.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22518..22889
                     /gene="rnpA"
                     /locus_tag="VWW99_000877"
     CDS             22518..22889
                     /gene="rnpA"
                     /locus_tag="VWW99_000877"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9538743.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22864..23688
                     /locus_tag="VWW99_000878"
     CDS             22864..23688
                     /locus_tag="VWW99_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9538744.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23707..>24464
                     /locus_tag="VWW99_000879"
     CDS             23707..>24464
                     /locus_tag="VWW99_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEU9538745.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTF"
BASE COUNT         7331 a         4300 c         5661 g         7172 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttattggggt atagccaagc ggtaaggcaa gggactttga
      301 ctccctcatg cgttggttcg aatccagcta ccccagttac tatttgccgg cgtggcggaa
      361 ttggcagacg cgctggactc aaaatccagt gtccgcaagg acgtgccggt tcgaccccgg
      421 ccgccggtat agtatagtgt taggaacgtt gttattcttc gttccttttt tatattattt
      481 ttggtataat tatagttatt caaattttat ttagattaag aaagtgtagg ggagtatgtc
      541 ttgttctatc gatttattaa aacatcggta tttgaaaaat attaaagaaa atcctgaatt
      601 gtttgtcgga attgagttgg agtatcctgt tgcaagttta gaaggggatg ctacagatgt
      661 tgaagttatg aaggatctat ttcattattt agtttctact ttggatctca ccgtagcaaa
      721 ggtagatgat tttggcaatc tgatccagtt agtagatccg ataagtcagg atgctatttt
      781 atttgaagtt tcctatacaa cgattgagtt tgcatttggt aaggctgaaa cgattcaaga
      841 ggtcgaaaat cgtttcaata attatatgaa tgtaattcag agaaagttag ctgaatcaaa
      901 tcatgctatt gttggctgtg gtatccatcc caactgggat aaaaatgaga attgtccagt
      961 ggcttatcca cgctatcaga tgttgatgga ttatttgaat ttgagtagaa atattattaa
     1021 atcagattta catcatttcc ctgaatatgg tacttttatc tgtgggagcc aggttcagct
     1081 ggatatttca aaaaccaact acttacgggt gattaatgct tttactcaaa ttgaagcggc
     1141 taaggcttat ttatttgcaa actctgaatt ttcgggtgcg gattgggata cgaaaatttc
     1201 aagggatatt ttctgggaag aatctatgca tggtatctat ccagagaatg ttggggtcaa
     1261 tgctagactc cttaatgatg aagctgattt ttttgactat ctaaatcatt ctgcgatttt
     1321 tactgcggaa cgtgatgggc agacctatta tttttatcct attcaggctg gggactattt
     1381 ggctacgtcc gaaatccaag catttgctct gaatggggat gaggttatta tttaccccca
     1441 agagaaggat tttgaaactc atcgtagtta ccagtaccaa gatttaacga ctcgaggaac
     1501 agttgagttt cgtagtgtgt gtacacagcc acttgatagg acttttgctt ctgcagcttt
     1561 tcacttggga ttattggtta atttagacaa gttagaagct tacttagaaa cagcaccttt
     1621 ctttaaagta tttggttatg attacaagtc tttaaggaga caattttcta agaaaaatct
     1681 tacagatgag gaagaaacta cgattattga attttccaaa gacttactcc tactagctga
     1741 ggagggacta gtggtgagaa ataaggaaga aatgacctat ttacagcctt tgagagaaga
     1801 attgagccta taatttctct tataaaggga gaattttctg aaaaatcatg atataatgga
     1861 cgagactata gataaaggat agagagtaat gacattagtt tatcaatcaa cgcgtgatgc
     1921 caacaataca gtaactgcca gccaagcaat tttgcaaggt ttggcgacgg acggcggttt
     1981 gtttacaccg gatacttatc caaaggtaga tttgaacttt gacaaattga aagatgcttc
     2041 ttaccaggaa gttgctaagc tagttttgtc agcattttta gatgacttta cagttgagga
     2101 gttggactac tgtatcaaca atgcctacga tagcaaattt gatactccag ctattgcacc
     2161 attagtgaaa ttagatgggc aatacaattt ggaacttttc catggttcaa cgattgcctt
     2221 taaggatatg gccttgtcta ttttgccata ctttatgacg actgctgcta agaaacatgg
     2281 tttggagaac aagattgtta tcttgacagc gacatctggt gacacgggga aagctgctat
     2341 ggcggggttt gcgaatgtgc ctggtactga gattatcgtc ttttatccaa aggatggtgt
     2401 cagcaagatt caagagttac aaatgaccac tcagactggc gacaatactc atgttattgc
     2461 tattgatggt aactttgacg atgcgcaaac aaatgtgaag cacatgttta acgacgtggc
     2521 tcttcgtgaa aaattgacta ccaacaagtt gcaattttca tcagctaact ctatgaacat
     2581 tggtcgtctg gtgccacaaa ttgtttatta tgtttatgct tacgctcaat tggttaagac
     2641 tggtgaaatt gtagctggtg aaaaggttaa cttcacagta ccaacaggaa actttggaaa
     2701 tatcttggct gccttttatg ccaaacaaat tggtttgcca gttggtaaat taatctgtgc
     2761 ttcaaatgac aacaatgttt tgacagactt ctttaaaaca cgtgtctatg acaaaaaacg
     2821 tgagtttaag gtaacaacca gcccatctat ggatatcttg gtatcttcaa acttggagcg
     2881 cttgattttc catcttttgg gaaataatgc tgaaaagaca actgaactta tgaatgcctt
     2941 gaacacgcaa ggacaatata agttgacaga ctttgatgca gagattttgg acctctttgc
     3001 agctgaatat gcgactgagg aagaaacggc agcagagatc aagcgtgttt gtgagttaga
     3061 ttcttatatc gaggaccctc atacagctgt tgcttcagca gtttataaaa aataccaatc
     3121 ggccactgga gatgtaacta agacagtgat tgcttcaaca gctagtccat acaagttccc
     3181 agtagttgca gtagaagctg taactggaaa agcaggttta acagactttg aagccttggc
     3241 tcaattacat gaaatctcag gcgttgcagt gccaccagca gttgatgggc ttgaaatagc
     3301 tccaattcgt cacaagacaa cagtggcagc tgctgacatg caagcagcgg ttgaggctta
     3361 tttaggactt taagacagag ggagcaaact cggttgggaa accaactgag tttcttttca
     3421 tcaggaggag agattgttta agaaaaataa agacattctt aatattgcat tgccagctat
     3481 gggtgaaaac tttttgcaga tactaatggg aatggtggac agttatttgg ttgctcattt
     3541 aggattgata gctatttcag gggtttcagt agctggtaat attatcacca tttatcaggc
     3601 gattttcatc gctctgggag ctgctatttc cagtgttatt tcaaaaagca tagggcagaa
     3661 agaccagtcg aagttggcct atcatgtgac tgaggcgttg aagattacct tactattaag
     3721 tttcctttta ggatttttgt ccatcttcgc tgggaaagag atgataggac ttttggggac
     3781 ggagagggat gtagctgaga gtggtggact gtatctatct ttggtaggcg gatcgattgt
     3841 tctcttaggt ttaatgacta gtctaggagc cttgattcgt gcaacgcata atccacgtct
     3901 gcctctctat gttagttttt tatccaatgc cttgaatatt cttttttcaa gtctagctat
     3961 ttttgttctg gatatgggga tagctggtgt tgcttggggg acaattgtgt ctcgtttggt
     4021 tggtcttgtg attttgtggt cacaattaaa actgccttat gggaagccaa cttttggttt
     4081 agataaggaa ctgttgacct tggctttacc agcagctgga gagcgactta tgatgagggc
     4141 tggagatgta gtgatcattg ccttggtcgt ttcttttggg acggaggcag ttgctgggaa
     4201 tgcaatcgga gaagtcttga cccagtttaa ctatatgcct gcctttggcg tcgctacggc
     4261 aacggtcatg ctgttggccc gagcagttgg agaggatgat tggaaaagag ttgctagttt
     4321 gagtaaacaa accttttggc tttctctgtt cctcatgttg cccctgtcct ttagtatata
     4381 tgtcttgggt gtaccattaa ctcatctcta tacgactgat tctctagcgg tggaggctag
     4441 tgttctagtg acactgtttt cactacttgg gacccctatg acgacaggaa cagtcatcta
     4501 tacggcagtc tggcagggat taggaaatgc acgcctccct ttttatgcga caagtatagg
     4561 aatgtggtgt atccgcattg ggacaggata tctgatgggg attgtgcttg gttggggctt
     4621 gcctggtatt tgggcagggt ctctcttgga taatggtttt cgctggttat ttctacgcta
     4681 tcgttaccag cgctatatga gcttgaaagg ataggaaatg caaaaaacag cttttatttg
     4741 ggatttagac gggactttat tggactctta cgaagcgatt ttatcaggga ttgaggagac
     4801 ttttgctcag ttttctattc cttatgataa ggagaaggtg agagagttta tcttcaagta
     4861 ttcggtgcaa gatttgcttg tgcgggtggc agaagataga aatctggatg ttgaggtgct
     4921 aaatcaggtg cgtgcccaga gtctggctga gaagaatgct caggtagttt tgatgccagg
     4981 tgcgcgtgag gtgctagctt gggcagacga atcaggaatt cagcagttta tatatactca
     5041 taaggggaac aacgctttta ccattctcaa ggacttgggg gtggaatcct attttacaga
     5101 gattttaacc agtcagagtg gctttgtgcg gaagccaagt ccagaagcgg ctacctatct
     5161 gctagataag tatcagttga attctgataa tacttattat ataggggatc ggactctgga
     5221 tgtggaattt gcccagaata gtgggattca aagcatcaac tttttagagt ctacttatga
     5281 agggaatcac aggattcaag cgttagcaga tatttcccgt atttttgaga ctaagtgata
     5341 aaaagattgt gtcagttttg tgacagagac ctaacaaact atttcaagta acctagtttg
     5401 ttacaaggaa tagacagttc tgttaaatag gcccgagagg gctttttttc tacatttttt
     5461 gtgttatgat agacaggtac tcatttgaaa ggaatttgaa agaatgaaga aaagaatgtt
     5521 attagcgtca acagtagcct tgtcatttgc cccagtattg gcaactcaag cagaagaagt
     5581 tctttggact gcacgtagtg ttgagcaaat ccaaaacgat ttgactaaaa cggacaacaa
     5641 aacaagttat accgtacagt atggtgatac tttgagcacc attgcagaag ccttgggtgt
     5701 agatgtaaca gtgcttgcga atctgaacaa aatcactaat atggacttga ttttcccaga
     5761 aactgttttg acaacgactg tcaatgaagc agaagaagta acagaagttg aaatccaaac
     5821 acctcaagca gactctagtg aagaagtgac aactgcgaca gcagatttga ccactaatca
     5881 agtgaccgtt gatgatcaaa ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagt
     5941 tacaaagaca gtgattgctt ctgaagaagt ggcaccatct acgggcactt ctgtcccaga
     6001 ggagcaaacg accgaaacaa ctcgcccagt tgaagaagca actcctcagg aaacgactcc
     6061 agctgagaag caggaaacac aagcaagccc tcaagctgca tcagcagtgg aagtaactac
     6121 aacaagttca gaagcaaaag aagtagcatc atcaaatgga gctacagcag cagtttctac
     6181 ttatcaacca gaagagacga aaataatttc aacaacttac gaggctccag ctgcgcccga
     6241 ttatgctgga cttgcagtag caaaatctga aaatgcaggt cttcaaccac aaacagctgc
     6301 ctttaaagaa gaaattgcta acttgtttgg cattacatcc tttagtggtt atcgtccagg
     6361 agacagtgga gatcacggaa aaggtttggc tatcgacttt atggtaccag aacgttcaga
     6421 attaggggat aagattgcgg aatatgctat tcaaaatatg gccagccgtg gcattagtta
     6481 catcatctgg aaacaacgtt tctatgctcc attcgatagc aaatatgggc cagctaacac
     6541 ttggaaccca atgccagacc gtggtagtgt gacagaaaat cactatgatc acgttcacgt
     6601 ttcaatgaat ggataaaccc gacttgataa catcattttg acgaatgaga tctagctttc
     6661 gtgatggaaa gcgattctcg ttcgtttttt ctttgtcata ctcttcgaaa atctcttcaa
     6721 accacgtcag ttttatctga aacttcaaag ctgtgctttg agcaacctgc gactagcttc
     6781 ctagtttgct ttttgatttt cattgagtat caatttgaat ggaaaatgga aagttatcat
     6841 cttgtaatga gttaagcaac attcttgcaa tctattttac tttatatcac aattaattag
     6901 tcaaatattg ataaatcaat aaaaagagag gggaagaaat gctagagatt caagatttac
     6961 tgtatcaact ccgcttgtct gagcaagcga gtacgcaatt gtttgaaaaa aggcttggga
     7021 ttagtttgac acggtatcag attttactgt ttttgctgga gcattctcct tgtaaccaaa
     7081 tggcggttca ggagcgtttg aaaattgatc aggctgcttt gacacggcat ttcaaaattt
     7141 tggaaacgga aggtttggtg gagcgtcatc gtaatcctga aaatcagcgg gaagtgttgg
     7201 tagaggctgc gaagtatgcc aaggagcagt tagtggtgaa tccccctctg caacatatca
     7261 gggttaagga agagatagaa agtatcttaa cagagtttga gagaacagaa ctcagccgtt
     7321 tattaaataa attggttttg ggtattgaaa atatagaaat ttaaggagaa atagatgtca
     7381 attattttaa caacgatcgt tgctttggag catttttaca ttttttattt ggaaagtatt
     7441 gccacgcaat cagatgcgac tagtcgtgta tttaatatgg aaaaggaaga attggctcat
     7501 ccgtcagtaa gttcattgtt caaaaatcaa ggaatttata aggctctgct aggagtcttt
     7561 ctcttgtatg tcatttattt ctcacagaat ttagaaattg tgactatttt tgtcttattt
     7621 gtgattggtg ctgcgactta cggctcttta acagcggata aaaaaattat tttgaaacaa
     7681 ggtggatcag ctattttggc cttgattagt attttactct ttaaatacac ttgaaggtcg
     7741 attctaatct cgctaatcct ttttaatcca gaataaggga aatatgttat acttgttttt
     7801 aagaaaaaag tctcattgaa ttggttttga ggagttagaa atgaaagtat tagtgacagg
     7861 ttttgagccc tttggagggg aaaagggcaa tccagctttg gaggccatta aaggtttacc
     7921 agctgaaatc catggtgctg aggtccgttg gctagaggtg ccgacagttt ttcacaaatc
     7981 tgctcaagta ttggaagaag agatgaatcg ttatcaacct gactttgtcc tttgtattgg
     8041 gcaagctggt ggaagaacta gtttgacacc tgaacgagtg accattaatc aagacgatgc
     8101 atgcatttct gataacgaag ataatcaacc gattgaccgt cccattcgcc cagatggtgc
     8161 ttcggcctac tttagtagtt tgccgattaa agcgatggtt caagctataa aaaaagaggg
     8221 cttaccggcc tctgtttcca atacggcagg gatttttgtc tgcagccatt tgatgtatca
     8281 ggctctctat ttggtagaaa agaaatctcc atatgttaag gcaggtttta tgcatattcc
     8341 ttatatgatg gaacaggtgg tgaacagacc gactactcca gctatgagtt tagtggatat
     8401 tcggcgaggg atagaagcag caatcggcgc tataatagaa catggagatc aggaactcaa
     8461 gttggtaggc ggagaaactc attgatagaa aaaagcttga ggggaaaaac cttcaagctt
     8521 ttggacgttt tcgggccaat actgctcggt aaaacataat tttagtgcat tggatataag
     8581 gtaggagtga aaaactagca atgccaaagg taatccaatt gaggaagtac caaggaagaa
     8641 gctgtaaatc taggacaaag tgctggaact tgtagccctt cataaaggaa cggctagttt
     8701 ttaggattcg tcttggtggg acctgtccta ggtctagact ataacagaga agaaattcca
     8761 cctgtgaata ggcataatac tgtggaatat agaggatatt tcctacaatg atcaagatga
     8821 gacttgcaag aaagtagagt ccaaagacca tgaggaaacg ctcggtttca actgatgaga
     8881 gatctagatt tggaaactca ggatgtaggg tgacgaattt tttggctaaa aagctactat
     8941 aaaagaggag gtaaatccca agtaaattag ggatactcca taaaaagaga tagaaacgtt
     9001 tgagaagtag ggtcaaaaag gtttgagaaa agcgctcctc atcaaagaga gctaggctgt
     9061 tttttacaga tggctccgtt ttagaatctt tcatgagtgt cagtgttgca tagacggaac
     9121 tggtcaaaag aatagtcccg ataaaggaga ctagtagagg aaagaggtag gtttgaagta
     9181 tttggccaag tatgctgaaa aatggctgtt ctaaaacagt cccgtggatc cgagataagg
     9241 gattaagaaa accagataag atgaccagca tactgggaag gatatagagg agaaagagac
     9301 ggggggtgtc agcctgaaaa tgttttgact cctgacgaat tgtttttaaa tcaatttttg
     9361 gatagttcat tctcttatta taccatagtt cttatacata gttcgtgaca gttcctactt
     9421 tttttgataa aatcatacag tgtgtccttg ggcacactgt atgaactggg actgtctttc
     9481 ccagcttcgg aggtaaaaaa tgtcagattc accaatcaaa tatcgtttga ttaagaaaga
     9541 aaaacacaca ggagctcgtc tgggagaaat catcactccc cacggtacct ttccgacacc
     9601 tatgtttatg ccagttggga cacaagccac tgtcaaaact cagtcacctg aagaattgaa
     9661 ggagatgggt tcgggaatta tcctatcaaa tacctatcat ctctggcttc gccctggaga
     9721 tgaactcatt gcacgcgctg gtggtctcca caagttcatg aattgggacc agcctatctt
     9781 gacagatagt ggtggttttc aggtttattc tttagcagat agccgtaata tcacagaaga
     9841 aggagtaacc tttaaaaatc atctaaatgg ttctaagatg ttcctatccc cagaaaaagc
     9901 catctctatt cagaataatc tgggttcaga catcatgatg tcctttgatg aatgtcctca
     9961 gttttatcaa ccttatgact acgttaagaa atcgatcgag cgtaccagcc gttgggctga
    10021 gcgtggtttg aaggctcacc gtcgtccaca tgaccaaggt ttgtttggaa ttgtgcaagg
    10081 tgcaggattt gaagaccttc gccgccaatc agctcatgat cttgtcagca tggatttctc
    10141 aggctactct atcggtggtt tggcagtggg agaaacccat gaagagatga atgcggtctt
    10201 ggactttaca actcaactgc tacctgaaaa taaacctcgt tatctgatgg gtgtgggagc
    10261 gccagatagc ttgatcgatg gggtcattcg tggggtggat atgtttgact gtgtcttacc
    10321 gactcgaatt gctcgtaacg ggacttgtat gaccagtcaa ggacgtttgg ttgtgaaaaa
    10381 tgcccagttt gctgaggact ttacgccact ggatcctgag tgtgactgct acacatgtaa
    10441 taactataca cgcgcttacc ttcgtcacct gctcaaggct gatgaaacct ttggtatccg
    10501 cttgactagc taccacaatc tttacttctt gcttaacctg atgaagcaag tgcgacaagc
    10561 catcatggat gacaatctct tggaattccg tgagtatttt gtggaaaaat atggctataa
    10621 taagtcagga cgtaatttct aaaatggaat tgatataaaa aaatcctaag ttttctctta
    10681 ggatttttct tctttttttg atagaataaa gtgtacaatg aaaggaagaa taaactcgta
    10741 tgcgcattaa atggttttcc ttgattagga ttataggttt acttttggta ctcttgtatc
    10801 acttctttca gacgatcttt cctggaggat ttttcggggt agatgtcttt ttcacatttt
    10861 caggtttcct gattacagct ctactcattg aagaattttc taagaacaat gagattgatt
    10921 tgataggatt ttttagaaga cgcttttatc ggattgtgcc acctgtggtt ttgatggtct
    10981 tggtgaccat gccttttact ttcttggttc gccaagacta tgttgctgga attggtggcc
    11041 agattgcggg cgtcttaggc tttatgacca acttctatga actcctaaca ggtgggagtt
    11101 atgaatctca gttcattcct catttgtttg ttcataattg gagtttggca gttgaggttc
    11161 actactatat tctttgggga ttggcagttt ggttcttatc caaacaagct aaatcaaatg
    11221 gtcagttgaa ggggatggtc tttctcttat ctgctgctgc cttcttgatc agtttcttct
    11281 ccatgtttat tggtagtttt ctagtgacct cttattcctc tgtttatttc tccagtttaa
    11341 ctcatgtcta tccattcttt ttgggaagta tgttagcaac tattgtaggt gttcgtcaga
    11401 cgacttccct cgtcaagcag ttggataaaa tctgggattt acgaaagact ttggtagttt
    11461 ttggaggagg ctttggtttc ctagttcttt tgactttctt tgtcaaattc acttatcttt
    11521 ttgcctatct tatcggcttc ttgcttgcca gtcttgcagc tcttgccatg attctggcgg
    11581 cgcgtgtctt acatgaaaag acacatcata tacaggagcc gaagattatc agctttttag
    11641 cggatactag ctatgcggtt tatcttttcc attggccttt ctatatcatt ttctcacagt
    11701 tgacatcaaa tcttcttgct gtattactga ctctgatttg ttcttatggc tttgccagtc
    11761 tgtcattcta tgtattggaa ccttggattg caggcaagaa cacacctatt ttacaaactc
    11821 ttcgtcccct gccttatatt cacgcaattc ttgtagcagg tacaggaatc ttgaccatca
    11881 ttgtctgcac ggtgaccttg ttggcaccac aagtgggagc gtttgagaca gacttgactg
    11941 tcaatggctt gaagcaagct gcaacaaata ttggccagac caaggtgatg gcagaacggg
    12001 cagatgcaaa cagtttggga attgctgatg gcactatgtt aattggtgac tcagtggctt
    12061 taagggcaaa tacagcacta cagacagctc ttcctggagc acagattaac gcgcaggtca
    12121 gcgtaacaac caagaccgca aatgaaatca tgctaaataa tagccagaat aaatttttac
    12181 ctaagacggt ggtcattgcg actggggtaa ataatcctga gaattacaag gatgactggg
    12241 acagtatcgt gaaaaatctt cctaagggac accatatgat tttggtgact ccttatgagg
    12301 gagataagac aaaagagacc tatgccatcg ttgagaaggc tgctgcctat atgagagaat
    12361 tggcagagaa gacaccttac attacgatag cagattggaa tcaagttgcg aaagagcatc
    12421 cagaaatttg ggctggaaca gaccaggttc atttcgggag tgagagtagc actatcgaag
    12481 caggagcaaa attgtatgca gatacgattg ccacagcttt gcagacagct caagacaagc
    12541 cggttaaatc aaaataactt gtattaaaaa agagaagagt tggagaaatc cagctctttt
    12601 aaaatatctc caaaaaatag gtctatacca tttacaaata aaaaagaaag gtttataatg
    12661 taattgacat aataaattgt agaatcaatc ttttaaggag gttaacatta tgcctaacta
    12721 tattaaagcg gatcagtttt tctacccaca cggagttcgt cgaggtggtt acttggaact
    12781 tgtggacggc aagtttggga aacatgtaga gcagattcct gaaggggctg aggtgattga
    12841 ctatacaggt tatagcattg ccccaggtct tgtggatact catattcatg gatatgcagg
    12901 tgtagatgtg atggacaaca acattgaagg tacattgcat actatgagtg aaggacttct
    12961 tagtaccggt gttaccagtt tcttacccac aactttaaca gccacttatg agcaattgct
    13021 tgcagtcact gaaaatcttg gaaaccatta taaagaagca acaggtgcta agattcgtgg
    13081 gatttattat gaaggtccat atttcacaga aacttttaag ggggcacaaa atccaactta
    13141 tatgagagac ccgggtgttg aggagtttca ttcttggcaa aaagcggcaa atggcttgct
    13201 taataaaatt gcccttgcac cagaacgtga tggggtggaa gactttgttc gtacagttac
    13261 gggcgaaggt gtgacggttg ctcttggaca ttcaaacgcg acttttgatg aagccaaaaa
    13321 agcagtcgat gctggagcga gtgtttgggt gcatgcctac aatggaatgc gtgggttgac
    13381 tcaccgtgaa ttgggtatgg ttggagccat gtaccaattg ccacatacct atgcagagtt
    13441 gatctgtgat ggtcaccacg tagatccaaa ggcttgcgaa attcttatca aacaaaaagg
    13501 aacagaaaac atcgctctta tcacagactg tatgacagct gggggattgg aagacggaga
    13561 ttatatgttg ggagaattcc cagttgtcgt tgcaaatgga actgcacgcc tcaaatcgac
    13621 aggtaacttg gcaggttcta tcctcaaact caaagatggt ttgaagaatg tggtcgaatg
    13681 gggaattgcg aatccgcatg aagcagtcat gatggccagc ttcaacccag ctaaatccgt
    13741 tcacatcgat gacgtctgtg gccaaatccg tgaaggctac gacgctgact tcatcgtatt
    13801 agataaagat ttggaattgg tagcaaccta tctagatggc gtaaaacgtt atcaagcata
    13861 agagagaagg cagaagttag agactagctt ctgctttttt atacagggta ctttactggt
    13921 aaataaaaag ttaaaaaaat cacaaaaaaa gcttgaagaa acaaagaaaa aggcttacaa
    13981 ttgaactata aatattacaa ataaaaaaac ggaggagtgc tttatgaaag cctatactta
    14041 tgttaaacca ggacttgctt cttttgttga tgtagacaaa ccagttattc gcaagccaac
    14101 agacgctatt gtgcgtattg taaaaaccac tatttgtgga acagacctcc atattatcaa
    14161 aggggatgtt cctacttgcc aaagtggtac cattcttggc cacgaaggga ttgggattgt
    14221 tgaagaagtt ggggaaggag tttccaactt caaaaaaggc gacaaggtct tgatttcttg
    14281 cgtctgtgcc tgtggtaaat gctactactg taaaaaagga atttatgccc actgtgaaga
    14341 cgaagggggc tggattttcg gtcacttgat tgatggtatg caggctgaat atctacgtgt
    14401 ccctcatgca gataatactc tttaccatac tccagaagac ttgtcagatg aagctttggt
    14461 tatgctgtca gacattctgc ctactggata tgaaattggt gtcttaaaag ggaaagtaga
    14521 acctggttgc agcgtagcca ttattggttc aggtccagtt ggattggctg ctcttttaac
    14581 agcccaattc tattcaccag ctaaattgat tatggtagac ctagacgata accgcttgga
    14641 aactgcccta tcattcggtg cgactcataa ggttaattct tcagacccta aaaaagccat
    14701 taaagaaatt tatgatttga cagatggtcg tggtgtggat gtcgctatcg aagctgtagg
    14761 gattcctgaa acatttgatt tctgtcaaaa gattatcggt gtagacggaa cggttgccaa
    14821 ctgtggtgtg catggtaaac cagttgaatt cgatttagat aaactttgga ttcgcaacat
    14881 caatgtaaca actggtttgg tatctacaaa tacgactcca caattgttga aagcacttga
    14941 aagtcataag attgaaccgg aaaaattggt aactcactat ttcaaactca gtgaaattga
    15001 aaaagcctac gaagtcttca gtaaggcagc agaccaccat gccattaagg tcattatcga
    15061 aaacgatatc tcagaagcct aagtagtaaa aatatttttg tacataagta aatagaaatt
    15121 cagtcatcca tcagatggtt ggatttttta tcaaaaaatt aagaaatgag catatttctt
    15181 tccttgtctg gcggaattgg ttataatata cggtacaaag gaatgaatga atatgtatcg
    15241 tgttatagaa atgtacggag attttgaacc gtggtggttc ttagaaggtt gggaagaaga
    15301 tattgtagca agtagaaaat ttgaccagta ttatgatgct ctcaaatact acaaaacttg
    15361 ctggtttaga ttggaacaag aatcgcctct ttataaaagt agaagcgact tgatgaccat
    15421 tttttgggac ccggaagacc aacgctggtg tgatgaatgt gatgagtatt tacaacaata
    15481 ccattctttg gctcttttgc aggatgagca ggttatccca gacgaaaaac tacgctcagg
    15541 ctatgaaaaa caaaccagtc aggaaaggaa tcgttcttgc cgtatgaaat taaaatagag
    15601 aaaagtaact ttttggagtt gcttttttta tttttctaac gctttgcgaa tagtataggt
    15661 gaggaggtaa gtatggttca agaaattgca caagaaatca ttcgttcggc tcggaaaaaa
    15721 ggggcgcaag acatttattt tgtccccaag ttagatgcct atgagcttca tatgagggta
    15781 ggagacgagc gctgtaaaat tggtagctat gattttgaaa agtttgcagc cgttatcagt
    15841 cactttaagt ttgtggcggg tatgaatgtg ggagaaaaaa gacgtagtca actgggttcc
    15901 tgtgattatg cctatgacca gaagatggcg tctctacgtt tatctactgt aggcgattat
    15961 cgggggcatg agagtttggt tatccgtttg ttgcacgatg aggagcagga cttgcatttt
    16021 tggtttcagg atattgaaga attaggcaag cagtacaggc aacggggact ctatcttttt
    16081 gctggtccgg ttgggagtgg taagacgacc ttgatgcatg aattgtccaa gtcactcttt
    16141 aaaggacagc aagttatgtc catcgaagat cctgtcgaaa tcaagcagga cgacatgctt
    16201 cagttgcagt tgaacgaagc aatcggccta acctatgaaa atctaatcaa actttccttg
    16261 cgtcatcgac cagatctctt gattatcgga gaaattcgtg acagcgagac ggcgcgtgca
    16321 gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa ccattcacgc caagagtatc
    16381 cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg aagaagaatt ggcagttgtt
    16441 ctgcaaggag tctgctacca gagattaatc gggggaggag gaatcgttga ctttgcaaac
    16501 agagattatc aagaacacca agcagccaag tggaatgagc aaattgacca gcttcttaaa
    16561 gatggacata tcacaagtct tcaggctgag acggaaaaaa ttagctacag ctaagcaaaa
    16621 aaatatcatc accctattta acaatctctt ttctagcggt tttcatctgg tggagactat
    16681 ctccttttta gataggagtt ccttgttgga caagcagtgt gtgacccaga tgcgtacagg
    16741 cttgtctcag gggaaatcat tctcagaaat gatggaaagt ttgggatgtt caagtaccat
    16801 tgtcactcag ttatctctag ctgaagttca tggcaatctt cacctgagtt tgggaaagat
    16861 agaagaatat ctagacaatc tggccaaggt caagaaaaag ttaattgaag tagcgaccta
    16921 tcctttgatt ttgctgggtt ttcttctctt aattatgctg gggctacgga attacctgct
    16981 cccacaactg gatagtagca atattgccac ccgaattatc ggtaatctgc cacaaatttt
    17041 tctaggcatg gtagggcttg tttccgtgct tgccctttta gcactaactt tttataaaag
    17101 aagttctaag atgagtgtct tttctatctt agcacgcctt ccctttattg gaatctttgt
    17161 gcagacctac ttgacagcct attatgcacg tgaatggggg aatatgattt cacagggaat
    17221 ggagctgacg cagatttttc aaatgatgca ggaacaaggt tcccagctct ttaaagaaat
    17281 cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa ttttctcaga cgataggaac
    17341 ctatcctttc tttaggaagg aattgagtct catcatagag tatggggaag ttaagtccaa
    17401 gctgggtagt gagttggaaa tctatgctga aaaaacttgg gaagcctttt ttacccgagt
    17461 caaccgcacc atgaatttgg tgcagccact ggtttttatc tttgtggcac tgattatcgt
    17521 tttactttat gcggcaatgc tcatgcccat gtatcaaaat atggaggtaa atttttaaaa
    17581 tgaaaaaaat gatgacattc ttgaaaaaag ctaaggttaa agcttttaca ttggtggaga
    17641 tgttagtagt cttgctgatt atcagcgtgc ttttcttgct ctttgtacct aatctgacca
    17701 agcaaaaaga agcagtcaat gacaaaggaa aagcagctgt tgttaaggtg gtggaaagcc
    17761 aggcagaact ttatagcttg gataaaaatg aagatgctac tctgagcaag ttacaaggag
    17821 atgggcgcat cacggaagaa caggttaaag cttataatga atactatact aaaaatggag
    17881 gggcaaatcg taaagtcaat gattaaggcc tttaccatgc tggaaagtct cttggttttg
    17941 ggacttgtga gtatccttgc cttgggctta tccagctctg tccagtccac ttttgcagca
    18001 gtagaggagc agattttctt tatggagttt gaagaactct atcgggaaac ccaaaaacgc
    18061 agtgtagcca gtcagcaaaa gactagtctg aacttggatg ggcagatgat tagcaatggc
    18121 agtcaaaagt tgacagttcc taaaggaatt caggcaccat caggccaaag tattacattt
    18181 gaccgagctg ggggcaattc gtccctggct aaggttgaat ttcagaccag taaaggagcg
    18241 attcgctatc aattatatct aggaaatgga aaaattaaac gcattaagga aacaaaaaat
    18301 tagggcagtg attttactgg aagcagtagt cgctctagct atctttgcca gcattgcgac
    18361 cctccttttg ggacaaattc aaaaaaatag gcaagaggaa gcaaaaatct tgcaaaagga
    18421 agaagtcttg agggtagcta agatggccct gcagacgggg caaaatcagg taagcatcaa
    18481 cggagttgag attcaggtat tttctagtga aaaaggattg gaggtctacc atggttcaga
    18541 acagttgttg gcaatcaaag agccataagg tcaaggcttt taccttgtta gaatccctgc
    18601 ttgccctcat tgtcatcagt gggggattac tcctttttca agctatgagt cagctcctca
    18661 tttcagaagt tcgctaccag caacaaagcg agcaaaagga gtggctcttg tttgtggacc
    18721 aacttgaggt agaattagac cgttcgcagt tcgaaaaagt agaaggcaat cgcctataca
    18781 tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa gtcagatgat ttccgtaaaa
    18841 cgaatgctcg tggtcgaggt tatcagccta tggtttatgg actcaaatct gtacggatta
    18901 cagaggacaa tcaactggtt cgctttcatt tccagttcca aaaaggctta gaaagggagt
    18961 tcatccatcg tgtggaaaaa gaaaaaagtt aaggcaggtg ttctcctcta cgcagtcacc
    19021 atagcagcca tctttagtct tttgttgcaa ttttatttga accgacaagt cgcccactat
    19081 caagactatg ctttgaataa agaaaaattg gttgcttttg ctatggctaa acgaaccaaa
    19141 gataaggttg agcaagaaag tggggaacag ttttttaatc taggtcaggt aagctatcaa
    19201 aacaagaaaa ctggcttagt gacgagggtt cgtacggata agagccaata tgagtttctg
    19261 tttccttcag tcaaaatcaa agaagagaaa agagataaaa aggaagaggt agcgaccgat
    19321 tcaagcgaaa aagtggagaa gaaaaaatca gaagagaagc ctgaaaagaa agagaattca
    19381 tagtcaattc aactataatg cgttgaatcc agaatagtcc actgtagttt ctagaaaatt
    19441 gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag tttactatac
    19501 tttgtgctaa attaaagata tgaaacatga ttttaaccac aaagcagaaa ctttcgattc
    19561 ccctaaaaat atcttcctcg caaacttggt atgtcaagca gccgagaaac agattgatct
    19621 tctatcagac aaagaaattt tagatttcgg tggtggcacg ggtctattag ccttgcccct
    19681 aacccctagc caagcaggct aagtcagtca ctcttgtaga catttctgag aaaatgttgg
    19741 agcaagctcg tttgaaagtg gagcagcaag caatcaagaa tatccagttt ttggagcaag
    19801 atttaccgaa aaatcccttg gagaaagagt ttgattgcct tgctgttagt cgggttcttc
    19861 atcatatgcc tgatttggat gcggctctct cactgtttca tcaacatttg aaggaagatg
    19921 ggaaactcat cattgctgat tttaccaaga cagaagctaa tcatcatgga tttgatttag
    19981 ctgaactgga aaacaagcta attgagcatg gtttttcatc tgtgcatagt cagattctct
    20041 atagtgctga agacctgttt caaggaaatc actcagaatt ctttttaata gtagcccaaa
    20101 aatcactcgc ctagtcaggg agtgattttt ctataaggat ggaaaaaaga agggaaattt
    20161 ggtaagatag gaatatggat tttgaaaaaa ttgaacaagc ttatacctat ttactagaga
    20221 atgtccaagt catccaaagt gatttggcga ccaactttta tgacgccttg gtggagcaaa
    20281 atagcatcta tctggatggt gaaactgagc taaaccaggt caaggagaac aatcaaaccc
    20341 ttaagcgttt agcactacgc aaagaagaat ggctcaagac ctaccagttt ctcttgatga
    20401 aggctgggca aacagaaccc ttgcaggcca atcaccagtt tacaccggat gctattgctt
    20461 tgcttttggt gtttattgtg gaagagttgt ttaaagagga ggaaattact atcctcgaaa
    20521 tgggttctgg gatgggaatt ctaggcgcta ttttcttgac ctcgcttact aaaaaggtgg
    20581 attacttggg aatggaagtg gatgatttgc tgattgatct ggcagctagc atggcagatg
    20641 taattggttt gcaggctggc tttgtccaag gagatgccgt tcgcccacaa atgctcaaag
    20701 aaagcgatgt ggtcatcagt gacttgcctg tcggctatta tcctgatgat gccgttgcgt
    20761 cgcgccatca agttgcttct agccaagaac atacttacgc ccatcacttg ctcatggaac
    20821 aagggcttaa gtacctcaag tcagacggat acgctatttt tctagctccg agtgatttgt
    20881 tgaccagtcc tcaaagtgat ttgttaaaag aatggctgaa agaagaggcg agtctggttg
    20941 ctatgattag tctgcctgaa aatctctttg ctaatgccaa acaatctaag actattttta
    21001 tcttacagaa gaaaaatgaa atagcagtag agccttttgt ttatccactt gctagcttgc
    21061 aagatgcaag tgttttaatg aaatttaaag aaaattttca aaaatggact caaggtactg
    21121 aaatataaaa tagattttgt tataatagtt gaaaacgctt aaaaaggggt atcatgttat
    21181 gacaaaaaca attgcaatca atgcaggaag ttcaagtttg aaatggcaat tatacttaat
    21241 gccagaagaa aaagtattgg cgaaaggttt gattgaacgt atcggtttga aagattcaat
    21301 ttcaactgta aaatttgacg gccgttctga acaacaaatt ttggatattg aaaatcatat
    21361 acaagccgtt aaaattttat tggatgactt gattcgtttc gatattatca aggcttatga
    21421 cgagattaca ggtgttggac atcgtgttgt tgctggtgga gaatatttca aagaatcaac
    21481 agttgttgag ggagatgttt tagaaaaagt tgaagagttg agtttgttgg ctcctctaca
    21541 caacccggcc aatgcagcag gtgttcgtgc cttcaaggaa ttgttgccag acattaccag
    21601 tgtagttgtt tttgatactt ccttccacac aagtatgcca gagaaagctt atcgctaccc
    21661 tctaccaaca aaatattaca cagaaaacaa ggttcgtaaa tacggtgctc atggtacaag
    21721 tcaccagttt gtagcaggag aagctgcaaa actcttggga cgtccattag aagacttgaa
    21781 gttaattacc tgtcatattg gtaacggagg ctcaattaca gctgtgaaag ccggcaaatc
    21841 tgtagacact tctatggggt tcactcctct tggtggtatt atgatgggaa cgcgtacagg
    21901 ggatattgat ccagctatca ttccttattt aatgcaatat acagaggatt ttaacacacc
    21961 agaagatatc agtcgtgttc ttaaccgtga atcaggtctt ttgggagttt ctgctaattc
    22021 tagcgatatg cgcgatatag aagcagctgt agcagaaggg aatcacgagg ctagcttggc
    22081 ttatgaaatg tatgttgacc gtatccaaaa acatatcggt cagtaccttg cagtgctaaa
    22141 tggagcagat gccattgttt tcacagcagg tgtcggtgaa aatgcagaga gtttccgtcg
    22201 tgatgtaatc tcagggattt cgtggtttgg ttgtgatgtt gatgatgaaa agaatgtctt
    22261 tggcgttaca ggagacatct caacagaggc agctaaaatc cgtgtcttgg ttattccaac
    22321 agatgaagaa ttagtcattg cccgtgacgt tgaacgcttg aaaaaataag tgaaactaaa
    22381 aaaatattca atacaaggag ttgggaaagt tatttttcca gcttcttttt ctgatgaaat
    22441 tgtccaaaac cttgctatga ttggcttttt tgaaaaatat ggtataatag tagtaattta
    22501 atagatggag ttgagttttg aagaaaaact ttcgtgtaaa aagagagaaa gattttaagg
    22561 cgattttcaa ggaggggaca agttttgcta atcgcaaatt tgtggtctac caattagaaa
    22621 accagaaaaa ccattttcga gtaggtctat cagttagcaa aaaactgggg aatgccgtca
    22681 ctagaaatca aattaagcga cggattcggc atattatcca gaatgcaaaa gggagtctgg
    22741 tagaagatgt cgactttgtt gtcattgctc gaaaaggagt cgaaaccttg ggatacgcag
    22801 agatggagaa aaatctactc catgtattaa aattatcaaa gatttaccgg gaaggaaatg
    22861 ggagtgaaaa agaaactaaa gttgactagt ttgctaggac tgtctctgtt aatcatgaca
    22921 gcctgtgcga ctaatggggt aactagcgat attacagccg aatcggctga tttttggagt
    22981 aaattggttt acttctttgc ggaaatcatt cgctttttat cgtttgatat tagtatcgga
    23041 gtggggatta ttctctttac ggtcttgatt cgtacagtcc tcttgccagt ctttcaggtg
    23101 caaatggtgg cttctaggaa aatgcaggaa gctcagccac gcattaaggc gcttcgagaa
    23161 caatatccag gtcgagatat ggaaagcaga accaaactag agcaggaaat gcgtaaagta
    23221 tttaaagaaa tgggtgtcag acagtcagac tctctttggc cgattttgat tcagatgccg
    23281 gttattttgg ccctgttcca agccctatca agagttgact ttttaaagac aggtcatttc
    23341 ttatggatta accttggtag tgtggataca acccttgttc ttccgatttt agcagcagta
    23401 ttcacctttt taagtacttg gttgtccaac aaagctttgt ctgaacgaaa tggcgctacg
    23461 actgcgatga tgtatgggat tccagtcttg atttttatct ttgcagttta tgcgccaggt
    23521 ggagtcgccc tatactggac agtgtctaat gcttatcaag tcttgcaaac ctatttcttg
    23581 aataatccat tcaagattat cgcagagcgc gaggccgtag tacaggcaca aaaagatttg
    23641 gaaaatagaa aaagaaaagc caagaaaaag gctcagaaaa cgaaataaat aaggaggaat
    23701 ctggtaatgg tagtatttac aggttcaact gttgaagaag caatccagaa aggattgaaa
    23761 gaattagata ttccaagaat gaaggctcat atcaaagtca tttctaggga gaaaaaaggc
    23821 tttcttggtc tatttggtaa aaaaccagcc caagtggata ttgaagcgat tagtgaaacg
    23881 actgttgtca aagcaaatca acaggtagta aaaggcgttc cgaaaaaaat caatgatttg
    23941 aacgagcctg tgaagacggt tagtgaagaa accgttgacc ttggtcatgt ggttaatgct
    24001 attaaaaaaa tagaggaaga aggtcaaggt atttctgatg aagtcaaggc tgaaatctta
    24061 aaacatgaaa gacatgccag cactatctta gaagaaactg gtcacattga gattttaaat
    24121 gaacttcaaa tcgaggaagc gatgagggaa gaagcaggcg ctgatgacct tgaaactgag
    24181 caagatcaaa ctgaaaatca agacttgaaa gagatgggct tgaaggtcga gcaaagttat
    24241 gatattgccc aggtggctac ggatgtgact gcctatgttc aagcgattgt ggatgacatg
    24301 gatgttgaag ctacactttc aaatgattat aaccgtcgta gcatcaatct acaaattgac
    24361 accaacgaac caggtcgtat tatcggctac catggtaaag tcttgaaggc cttgcaactg
    24421 ttggctcaaa attatcttta caaccgctat tccaaaacct tcta
//
LOCUS       DAVKHZ010000023        24264 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000023 DAVKHZ010000000
VERSION     DAVKHZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.023"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            725..2878
                     /locus_tag="VWW99_000880"
     CDS             725..2878
                     /locus_tag="VWW99_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9538746.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2891..4240
                     /locus_tag="VWW99_000881"
     CDS             2891..4240
                     /locus_tag="VWW99_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9538747.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSFYQSYK
                     SQGEENPQTKVQVVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4410..5117
                     /locus_tag="VWW99_000882"
     CDS             4410..5117
                     /locus_tag="VWW99_000882"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9538748.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5174..8899
                     /locus_tag="VWW99_000883"
     CDS             5174..8899
                     /locus_tag="VWW99_000883"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9538749.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELCDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8992..10434
                     /locus_tag="VWW99_000884"
     CDS             8992..10434
                     /locus_tag="VWW99_000884"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9538750.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNTASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10471..11493
                     /locus_tag="VWW99_000885"
     CDS             10471..11493
                     /locus_tag="VWW99_000885"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9538751.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11490..12035
                     /gene="purN"
                     /locus_tag="VWW99_000886"
     CDS             11490..12035
                     /gene="purN"
                     /locus_tag="VWW99_000886"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9538752.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12119..12628
                     /locus_tag="VWW99_000887"
     CDS             12119..12628
                     /locus_tag="VWW99_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9538753.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEY
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12653..14200
                     /gene="purH"
                     /locus_tag="VWW99_000888"
     CDS             12653..14200
                     /gene="purH"
                     /locus_tag="VWW99_000888"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9538754.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLGSDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14322..15584
                     /gene="purD"
                     /locus_tag="VWW99_000889"
     CDS             14322..15584
                     /gene="purD"
                     /locus_tag="VWW99_000889"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9538755.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAADTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            15986..16474
                     /gene="purE"
                     /locus_tag="VWW99_000890"
     CDS             15986..16474
                     /gene="purE"
                     /locus_tag="VWW99_000890"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9538756.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEAYSRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16461..17552
                     /gene="purK"
                     /locus_tag="VWW99_000891"
     CDS             16461..17552
                     /gene="purK"
                     /locus_tag="VWW99_000891"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9538757.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALAESADCVLEEFVNFALEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17562..17789
                     /locus_tag="VWW99_000892"
     CDS             17562..17789
                     /locus_tag="VWW99_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9538758.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17852..19150
                     /gene="purB"
                     /locus_tag="VWW99_000893"
     CDS             17852..19150
                     /gene="purB"
                     /locus_tag="VWW99_000893"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9538759.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19205..23143)
                     /gene="strH"
                     /locus_tag="VWW99_000894"
     CDS             complement(19205..23143)
                     /gene="strH"
                     /locus_tag="VWW99_000894"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9538760.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEKTPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYDSDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGSPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23457..24173)
                     /locus_tag="VWW99_000895"
     CDS             complement(23457..24173)
                     /locus_tag="VWW99_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9538761.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         6616 a         4788 c         5732 g         7128 t
ORIGIN      
        1 acaattaaaa tagaaacaat attgacagtg atttctgttg ttttcacatt tatagcaact
       61 cttattgaat atggattttc tctctttctg attacattat tttttgataa gaataagagg
      121 aactttggag atattctttt atctaaaaat ataaccttaa gttttagcat tttactttgt
      181 tatttattta atccatcgga tataaagttt gcctatttca gcgtattgac agtattacta
      241 attgggataa caaattatgc gagatttaaa aatttaagat tgacagtagt gttttctgta
      301 ccatttttat ttaatgctat aatgtctatt ataaatagtt tttagaaata ctaaaatttt
      361 tttctttact taataatgat atgaaatcat ttcggttttg ttaacttgta attcttatat
      421 tttctaggta gaacgtttgt tctgcctatt tttttatcca aaaagtgcag ttgggaggga
      481 gataggctca tttgggaagg aagtccagtt tttgtttagt gattggggta agatagttgt
      541 tatcagatga gtttatactc ttcgaaaatc aaattcaaac cacgtcaacg tcgccttgcc
      601 gtatatatgt gactgacttc gtcagtccta tctacaacct caaaacagtg ttttgagcag
      661 cctgcggcta gtttcctagt ttgctctttg attttcattg agtattaggg aaaaggagat
      721 gaatatgaaa tttgggaaac gtcactatcg tccgcaagtg gatcagatgg actgcggtgt
      781 agcttcatta gccatggttt ttggctacta tggtagttat tattttttgg ctcacttgcg
      841 agaattggct aagacgacca tggatgggac gacggctttg ggcttggtca aggtggcaga
      901 ggagattggt tttgagacgc gagccattaa ggcggatatg acgctttttg acttgccgga
      961 tttgactttt ccttttgttg cccatgtgct taaggaaggg aaattgctcc actactatgt
     1021 ggtgacgggg caggataagg atagcattca tattgccgat ccagatcccg gggtgaagtt
     1081 gactaaactg ccacgtgagc gttttgagga agaatggaca ggagtgactc tttttatggc
     1141 acctagtcca gactataagc ctcataagga acaaaaaaat ggtctgctct cttttatccc
     1201 tatattagtg aagcagcgtg gcttgattgc caatatcgtt ttggcaacac tcttggtaac
     1261 cgtgattaac attgtgggtt cttattatct gcagtctatc attgatacct atgtgccaga
     1321 tcagatgcgt tcgacactag ggattatttc tattgggcta gtcatcgtct acatcctcca
     1381 gcaaatcttg tcttacgctc aggagtatct cttgcttgtt ttggggcaac gcttgtcgat
     1441 tgacgtgatt ttgtcctata tcaagcatgt ttttcacctc cctatgtcct tctttgcgac
     1501 acgcaggaca ggggagatcg tgtctcgttt tacagatgct aacagtatca tcgatgcgct
     1561 ggcttcgacc atcctttcga ttttcctaga tgtgtcaacg gttgtcatta tttcccttgt
     1621 tctattttca caaaatacca atctcttttt catgacttta ttggcgcttc ctatctacac
     1681 agtgattatc tttgccttta tgaagccgtt tgaaaagatg aatcgggata ccatggaagc
     1741 caatgcggtt ctgtcttctt ctatcattga ggacatcaac ggtattgaga ctatcaagtc
     1801 cttgaccagt gaaagtcagc gttaccaaaa aattgacaag gaatttgtgg attatctgaa
     1861 gaaatccttt acctatagtc gagcagagag tcagcaaaag gctctgaaaa aggttgccca
     1921 tctcttgctt aatgtcggca ttctctggat gggggctgtt ctggtcatgg atggcaagat
     1981 gagtttgggg cagttgatta cctataatac cttgctggtt tactttacca atcctttgga
     2041 aaatatcatc aatctgcaaa ccaagcttca gacagcgcag gttgccaata accgtctaaa
     2101 tgaagtgtat ctagtagctt ctgagtttga ggagaagaaa acagttgagg atttgagctt
     2161 gatgaaggga gatatgacct tcaagcaggt tcattacaag tatggctatg gtcgagacgt
     2221 cttgtcggat atcaatttaa ccgttcccca agggtctaag gtggcttttg tggggatttc
     2281 agggtcaggt aagacgactt tggccaagat gatggttaat ttttacgacc caagtcaagg
     2341 ggagattagt ctgggtggtg tcaatctcaa tcagattgat aaaaaagccc tgcgccagta
     2401 catcaactat ctgcctcaac agccctatgt ctttaacgga acgattttgg agaatcttct
     2461 tttgggagcc aaggagggga cgacacagga agatatctta cgggcggtcg aattggcaga
     2521 gattcgagag gatatcgagc gcatgccact gaattatcag acagaattga cttcggatgg
     2581 ggcagggatt tcaggtggtc aacgtcagag aatcgctttg gcgcgtgctc tcttgacaga
     2641 tgcgccggtc ttgattttgg atgaggcgac tagcagtttg gatattttga cagagaagcg
     2701 gattgtcgat aatctcattg ctttggacaa gaccttgatt ttcattgctc accgcttgac
     2761 tattgctgag cggacagaga aggtggttgt cttggatcag ggcaagattg tcgaagaagg
     2821 aaagcatgct gatttgcttg cacagggagg cttttacgcc catttggtca atagctagaa
     2881 agaggagagg atgaaaccag aatttttaga aagtgcggag ttttataatc gtcgttacca
     2941 taatttttcc agtagtgtga ttgtacccat ggcccttctg ctcgtgtttt tacttggctt
     3001 tgcaactgtt gcagagaagg agatgagttt gtccactaga gctactgtcg aacccagtcg
     3061 tatccttgca aatatccagt caactagcaa caatcgtatt cttgtcaatc atttggaaga
     3121 aaataagctg gttaagaagg gggatctttt ggttcaatac caagaagggg cagagggtgt
     3181 ccaagcggag tcctatgcca gtcagttgga catgctaaag gatcaaaaaa agcaattgga
     3241 gtatctgcaa aagagcctgc aagaagggga gaaccacttt ccagaggagg ataagtttgg
     3301 ctaccaagcc acctttcgcg actacatcag tcaagcaggc agtcttaggg ctagtacatc
     3361 gcaacaaaat gagaccatcg cgtcccagaa tgcagcagct agccaaaccc aagccgaaat
     3421 cggcaacctc atcagtcaaa cagaggctaa aattcgcgat taccagacag ctaagtcagc
     3481 tattgaaaca ggtgcttcct tggccggtca gaatctagcc tactcttttt atcagtccta
     3541 caagtctcag ggcgaggaaa atccccaaac taaggttcag gtagttgcac aggttgaagc
     3601 acagatttct cagttagaat ctagtcttgc tacttaccgt gtccagtatg caggttcagg
     3661 tacccagcaa gcctatgcgt cagggttaag cagtcaattg gaatccctta aatcccaaca
     3721 cttggcaaag gttggtcagg aattgaccct tctagcccag aaaatcttgg aggcagagtc
     3781 aggtaagaag gtacagggaa atcttttaga caaggggaaa attacggcga gtgaggatgg
     3841 ggtgcttcat cttaatcctg agaccagtga ttctagcatg gttgcagaag gtgccctact
     3901 agcccaactt tatccatctt tggaaagaga aggtaaagcc aaactcacag cttatctaag
     3961 ttcaaaagat gtagcaagaa tcaaggtcgg tgattctgtt cgctatacta cgactcatga
     4021 tgccgggaat caacttttcc tagattctac tattacaagt attgatgcga cagctactaa
     4081 gactgagaaa gggaatttct ttaaaatcga ggcggagact aatctaactt cggagcaggc
     4141 tgaaaaactt aggtacgggg tggaaggccg cttgcagatg attacgggca agaaaagtta
     4201 tctacgttat tatttggatc aatttttgaa caaagagtaa tgttcgtgtt tttagagtta
     4261 aataattttt aaactgtgag aaagattctt cttgcagttt tttctttaca atttttggaa
     4321 aacatctgct atttattcgg ttaaattctt gtattttttg gttttttgtg gtaaaatgtg
     4381 ctcaagtaat acgaaaggcg aactttaaaa tgtcaaaaca attgatctat tcgggaaaag
     4441 ctaaagatat ctatacaact gaggatgaaa atcttattat ttcaacttac aaggaccagg
     4501 cgactgcttt caacggtgtc aagaaggagc agattgcagg taagggagtc ttgaataatc
     4561 agatttcatc ttttattttt gagaaattaa atgcggctgg tgtggcgact cactttgtgg
     4621 agaaactttc agacacggaa caactcaata aaaaggttaa gattattcct ttggaagtcg
     4681 tgctccgcaa ttatactgct ggttcctttt caaaacgttt tggtgtggat gagggaatcg
     4741 ccttggagac tccgattgtc gaattttact acaaaaatga tgatttggat gatccattta
     4801 tcaatgatga gcatgtgaaa ttcctacaga ttgcggatga ccagcagatt gcctacttga
     4861 aggaagaaac gcgtcgtatc aatgaactat tgaaagtctg gtttgctgag attgggctta
     4921 aattgattga ctttaagcta gagttcggtt ttgacaagga tggcaagatt atcttggcag
     4981 acgaattttc accagataac tgccgcttgt gggatgctga tggcaaccac atggataagg
     5041 atgttttccg tagaggattg ggagaactaa ccgacgttta tgagattgtt tgggaaaagt
     5101 tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag ctgaaataaa
     5161 ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt ttcaggtcaa
     5221 gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct tgaaaagtat
     5281 tcgtattgtg caagtatatg atgtatttga cttggttgag gacttgtttg cacctgcaga
     5341 gaagcacatt ttctctgagc aggtaaccga ccatgtttta gatgaagtat ctgtgcaggc
     5401 ggatcttgct aactatgctt tctttgccat tgaaagtcta ccagggcagt ttgaccagcg
     5461 tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg tgacagtcaa
     5521 cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg aagctgtcaa
     5581 aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgatag ggattgccaa
     5641 gcaggagttt tcagagtcag acaagaccat tccaaaattg cctttctttg aaagctatac
     5701 agcagaagac tttgctcgct acaaggccga acaagggatg gccatggaag tggatgattt
     5761 gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga ctgaactcaa
     5821 ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga cagagttgaa
     5881 acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct atgacaagta
     5941 tattgccatg cgcgaggaat taggtcggtc tgaaaaacca caaaccttga tggatatggc
     6001 gactattttc ggtcgttatg agcgtgctaa tggacgattg gatgatatgg aagtctctga
     6061 cgaaatcaat gcctgctcag ttgaaattga agtggacgtt gatggtgtca aggaaccttg
     6121 gctcctcatg tttaaaaacg aaacccacaa ccatccaaca gaaattgagc catttggtgg
     6181 agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt cctatgttta
     6241 ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg aaactcgtgc
     6301 tgggaaattg ccacaacaag tcatttctaa aacagcaact catggttatt cttcatatgg
     6361 taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag gctttgtagc
     6421 taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg ttgtccgtga
     6481 aaaacctgaa gcaggtgatg tgatcatcct tctcggaggt aaaacaggtc gtgatggtgt
     6541 cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga ctgctggtgc
     6601 tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct tccgtaatgg
     6661 caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggtg tctgtgtggc
     6721 tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc ttaaatacca
     6781 gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg cggtcgtggt
     6841 tcgtcctgaa gatgtggatg ccttcgttgc cgaatgtaac aaagaaaata ttgatgctgt
     6901 tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg gtgagacaat
     6961 cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg tcgatgccaa
     7021 agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg aaacactgga
     7081 atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag gattacagac
     7141 tgtctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg gtcgttacca
     7201 actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg tgactcatac
     7261 tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc cataccacgg
     7321 tgctgcttac gcggttatcg aagcaactgc tcgtttggtg gctgctggtg ccaactggtc
     7381 taaggctcgt ttctcttacc aagaatattt cgagcggatg gacaagcaag cagagcgttt
     7441 tggtcagcca gtagccgccc ttctaggctc tattgaagct cagattcaac ttggcttgcc
     7501 atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg ttccgccaac
     7561 cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc cagaatttaa
     7621 agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag agattgattt
     7681 tgacttgatt aagaaaaatt ttgctcagtt tgaagcaagc caagctgacc ataaagtgac
     7741 atctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg ctacctttgg
     7801 aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga cagctcaatt
     7861 aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaaga ttggacaaac
     7921 gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca agcttgacag
     7981 tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag cgaaagaact
     8041 agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa aggttgaaaa
     8101 acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt cagctaaggc
     8161 cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga atgaagaagc
     8221 tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata ttctcttctt
     8281 tgccggtgga ttctcggctg cggatgaacc agatggttca gctaagttta tcgtcaatat
     8341 cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg gtggcttgat
     8401 tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac catacggaaa
     8461 ctttgaagat gctaacagta ctagtccaac cctcttctac aatgatgcca accaacacgt
     8521 ggccaagatg gtggaaactc gcattgccaa taccaactca ccatggttgg ctggtgtgca
     8581 agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg tcgtgacggc
     8641 tgaggaattt gcagagctct gtgacaatgg acaaattttc agccaatacg ttgactttaa
     8701 cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg ccatcgaagg
     8761 aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac gttatgagga
     8821 tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat cagcggttaa
     8881 acatttcact ggaaaataag acttacagat tttctaatag atagtatcag taatgtaaaa
     8941 gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa aatgacatac
     9001 gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca tccagatgct
     9061 gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga gggggcagga
     9121 atcctctcca acgatcaagg acaactgaag cgccatcgtg acatggggct tttatcagaa
     9181 gttttcagaa atccagctaa tttggataaa ttgacaggag ctggtgcgat tgggcatgtg
     9241 cgttatgcga ctgctggcga agcttctgta gataacatcc agcccttcct cttccgtttt
     9301 cacgatatgc agtttggctt ggctcataat ggaaatctga ccaatacagc ctctctcaag
     9361 aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga aatcttggct
     9421 cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga agcgctcagc
     9481 cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat tgcggctctt
     9541 gaccctaatg gctttcgtcc gctttctatc gggaaaatgg ccaacggagc ggtggttgtt
     9601 tcctctgaaa cctgtgcttt tgaggtcatt ggtgccgagt ggattcgtga tttgaagcca
     9661 ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac agatgatacc
     9721 cagttggcga tttgttctat ggagtatatc tactttgccc gccctgattc taatatccac
     9781 ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg agaatttaag
     9841 catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc tatgggattt
     9901 gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata cacccagcga
     9961 acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa actgtctgct
    10021 gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat tgtacgtgga
    10081 acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga ggttcacgtt
    10141 gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat ccagacccgt
    10201 caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat tggtgcggac
    10261 agtctgactt atctttcaat tgatggcttg attgagtcga ttggtatcga aacagatgcg
    10321 ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc tctttatgac
    10381 tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa gtaggcgaca
    10441 gattctccat taaagaaaag gaaaaaacaa atgacaaata aaaatgcata tgcccagtcg
    10501 ggtgtggatg ttgaagcggg ttatgaagtt gttgaacgga ttaaaaagca cgtggcccgt
    10561 acggagcgtg caggtgtcat gggagctctt ggtggctttg gtggtatgtt tgacctttcc
    10621 aagactgggg ttaaagaacc cgtcttgatt tcagggactg acggtgtcgg aaccaagctc
    10681 atgttggcta tcaagtacga caagcacgat accatcgggc aggactgtgt ggccatgtgt
    10741 gtcaacgaca tcattgctgc aggtgcggaa cccctctatt ttctcgacta cgtagcgaca
    10801 gggaagaatg aaccagctaa gctagaacaa gtggttgctg gtgtggcaga aggttgtgtg
    10861 caggctggtg ctgccctcat cggtggggaa acggctgaaa tgccgggcat gtacggcgaa
    10921 gacgactatg acttggctgg ttttgcggtc ggtgtggctg aaaaatctca aatcattgac
    10981 ggttcaaagg tggtagaggg agatgttctt ctcggacttg cttcaagtgg gattcactca
    11041 aatggttact ctttggttcg tcgtgtcttt gcggattaca caggtgagga agtcctacca
    11101 gaattggaag gcaagaaact taaggaagtt ctacttgagc cgactcgtat ctatgtcaag
    11161 gctgtcttgc cgctcatcaa agaagagttg gtcaacggca ttgcccacat cacaggtggt
    11221 ggctttatcg aaaatgtccc tcgtatgttt gcagatgacc tagctgctga aattgatgaa
    11281 agtaaagttc cagtgcttcc aattttcaaa acccttgaaa aatacggtca gattaaacac
    11341 gaagaaatgt ttgaaatctt caatatgggt gtgggactca tgttggcggt cagccctgaa
    11401 aatgtagagc gtgtaaaaga attgttggat gaagcagtct atgaaattgg tcgcatcgtc
    11461 aagaaagaaa acgaaagtgt cattatcaaa tgaaaaaaat agcggttttt gcctctggta
    11521 atggctcaaa ttttcaggtg attgccgaag aatttccagt ggagtttgtc ttttcagacc
    11581 atcgtgatgc ctatgtgctt gagcgtgcaa agcagctcgg cgttctgtcc tatgcttttg
    11641 aactcaagga gtttgagagc aaggcagact acgaagcagc ccttgtcgaa ctcttggaag
    11701 aacaccagat tgacttggtt tgcctagcag gctacatgaa aatcgttgga ccaaccttat
    11761 tgtcggctta tgaaggtcgg attgtcaaca ttcatccagc ctacttgcca gaatttccag
    11821 gagctcatgg gattgaggat gcttggaatg ctggcgtggg tcagtctggt gtgaccattc
    11881 actgggtgga ttcgggtgtg gatacaggcc aggtcatcaa acaggttcgt gtgccacgac
    11941 tagctgatga taccattgac agatttgaag ctcgcatcca tgaagcagag tacaggttgt
    12001 atccggaagt agtgaaggct ctatttacag attgactttt tgatgattca tatgatatct
    12061 ttgattttaa attggagtca gtgtttgttg aagacggctt caaacggagg tatttgtaat
    12121 gttagaatct aaaaaaacaa ctcgatatgt attttatgtc tatctgatgt tattaacttg
    12181 gggaatctta tttaagtttg aaacaaatcc tgaatttata gcatttttct tagctccaag
    12241 gtatatcaat tggattccat tttcagaacc actaatagtc gatggaaaaa ttgtttttgc
    12301 tgaaatgtta tttaatctga ttttctttat tccattaggt gtttgtttcc ctttgataaa
    12361 aactaattta tctagtttaa gaatagtcgg gacaggtttc ttgattagtt tattgtttga
    12421 gtacttacag tatattttag caataggtat aacagatata acggatttga ctttaaatac
    12481 gctaggtgtc tgtgtaggct tactgattta tcaaattttt ataagagtgt tcaaatcaca
    12541 gactagaaaa tggatcaata tcttaggtat gcttagcctt ggttttgctt atcttgtttt
    12601 actgttactg catttaattg gtgtttaact aatgattaaa aaggagaata taatgactaa
    12661 acgcgtctta atcagcgtct cagacaaagc gggcattgtt gaatttgccc aagaactcaa
    12721 aaaacttggt tgggagatta tctcaacagg tggaactaag gttgcccttg ataatgctgg
    12781 ggtggatacc attgctatcg atgatgtaac tggtttccca gaaatgatgg acggtcgtgt
    12841 gaagaccctc cacccaaata tccacggagg gcttctcgct cgtcgtgact tggatagcca
    12901 cttggaagcg gctaaggaca acaagattga gctcattgac cttgtggtgg tcaaccttta
    12961 cccatttaag gaaactatcc ttaaaccaga tgtgacttat gctgatgcag ttgaaaatat
    13021 cgatattggt gggccgtcta tgcttcgttc agcagcgaaa aatcatgcca gtgttacagt
    13081 tgtggtagat cctgctgact acgctgtggt tttggacgaa ttggcagcaa acggcgaaac
    13141 ctcttatgaa actcgccaac gtttagcagc caaagtattt cgtcacacag cggcttatga
    13201 cgccttgatt gcagaatact tcacagctca agtgggtgaa agcaagcctg aaaaactcac
    13261 tttgacttat gacctcaagc aaccaatgcg ttacggtgag aatcctcaac aagacgcgga
    13321 cttttaccag aaagctttgc ctacagatta ctctattgct tcagccaaac agctcaacgg
    13381 gaaagaattg tcatttaata atatccgtga tgcagatgct gctatccgta tcatccgtga
    13441 cttcaaagat agtccaaccg ttgtggctct caaacacatg aatccatgtg gaattggtca
    13501 agctgatgac atcgagactg cttgggacta cgcttatgag tctgacccag tatctatctt
    13561 tggtgggatt gtcgtcctca accgtgaggt ggatgctgcg acagctgaga agatgcacgg
    13621 cgttttcctc gaaatcatca ttgcaccaag ctatacggat gaagcgctag ccattttgat
    13681 caataaaaag aaaaacttgc gtatccttgc cttgccattt aatgctcaag aggctagcga
    13741 agtggaagca gaatacacag gtgtagtcgg tggacttctc gtgcaaaatc aagacgtggt
    13801 caaggaaagc ccagctgact ggcaagtggt gaccaaacgt cagccaactg agacagaagc
    13861 gactgctctt gagttcgctt ggaaggctat caagtacgtc aaatcaaatg gtattatcgt
    13921 gaccaacgac cacatgacac ttggtgttgg tccaggtcaa accaaccgtg tggcttctgt
    13981 tcgccttgcc attgaccaag ccaaagatcg tctgaacggg gcggtccttg gttcagatgc
    14041 cttcttccca tttgcggata acgtggaaga aatcgccaaa gcaggaatta aggccatcat
    14101 ccagcccggt ggctctgtcc gtgaccaaga atccatcgaa gcagcggata aatacggctt
    14161 gactatggtc tttacaggtg tgagacattt tagacattaa gaagataaaa gggaagaaaa
    14221 cagtttcttt ccttttttgg cttaaaatgc taactgaaac aagattaaaa cgaacttttt
    14281 tgatataatg ttggtaaata attcgcaaaa gaggttgagg aatgaaactg cttgttgtcg
    14341 gttctggtgg tcgtgagcat gcgattgcta aaaagttact tgaatcaaaa gacgtggaaa
    14401 aagtctttgt agctcctggg aatgacggta tgactctgga tggtctggaa ttggtaaata
    14461 tatctatttc cgaacattat aaattgattg acttcgcaaa ggccaacgat attgcttgga
    14521 cctttatcgg tccagatgac gcccttgcgg ctggtatcgt ggatgatttc catgcagctg
    14581 gtctcaaagc ctttggtccg acaagattgg cagcggagct ggagtggtcc aaggatttcg
    14641 ccaaggaaat catggtcaaa tatggtgttt cgacagcagc ctatggcaca ttttcagatt
    14701 tcgaggaagc aaaggcctat atcgaaaagc atggtgcgcc tatcgtagtc aaggcggatg
    14761 gcttggcgct tgggaagggt gtcgtcgttg ctgagacggt tgagcaagcg gtcgaagccg
    14821 ctcatgagat gcttttggac aataaatttg gtgactcagg tgcgcgcgtg gttattgagg
    14881 aattccttga aggagaggaa ttttcactct ttgcctttgt caatggtgat aagttctaca
    14941 tcatgccaac ggctcaggac cacaaacgtg cctacgatgg cgacaaaggg cctaacacgg
    15001 gtggtatggg tgcctatgcg ccagttccac acttaccaca gagtgtagtt gatacagcgg
    15061 ctgacaccat tgtcaagcca gttctagaag ggatgattaa agaaggtcgc ccttatctgg
    15121 gagttcttta cgcagggctt atcctgacag ctgatggacc gaaagtcatt gagttcaacg
    15181 ctcggttcgg agatccagaa actcagatta tcttgcctcg cttgacctct gactttgctc
    15241 aaaatatcac agatatcctg gatagcaagg agccaaatat catgtggacg gacaagggtg
    15301 tgactctggg tgtggttgtc gcatccaagg gctacccgct agactatgaa aggggcgttg
    15361 agttgccagc caagacagaa ggcgatgtca tcacctacta tgcaggggct aagtttgcgg
    15421 aaaatagcag agcactgctc tcaaacggcg gacgagttta tatgctcgtt accacagcag
    15481 ataccgtcaa agaagcccaa gccagcattt accaagaact ataccaacaa aaaatagaag
    15541 gactcttcta ccgaacagat atcggaagca aggcaattaa gtaaagatat aagaataacg
    15601 cgccgtagtc gccaaacacg ataatggtcg tcgtggtgaa aagaccagaa cagtgaatgt
    15661 tctggtcagg ggaaacttgg agaccttagg ctcaaagttt aggaatgaaa ccgaaggttt
    15721 gcttccgcct ccatcaccta agaccattat caaaaagaaa aataaaaatt cacaaaatac
    15781 gttaatgatc gtatggtttg cgagcgttag cgagctaata tagaacaatc accgccgttg
    15841 tgaaagaacg attggatgat aatccaatcg ttcagggaaa ttggaagacc ttgggtttcc
    15901 aatttaggca tgagacacct ttggtggctg ctgccgtccc tcacaagcta aggtgattgt
    15961 tgaaaaagag gaaaaaggag aagaaatgaa accagtaatt tccatcatca tgggctcaaa
    16021 atccgactgg gcaaccatgc aaaaaacagc agaagttcta gaccgcttcg gtgtagccta
    16081 cgaaaagaaa gttgtttccg cacaccgtac accagacctc atgttcaaac atgcagaaga
    16141 agcatatagc cgtggtatta aggtcatcat cgcaggtgct ggaggcgcag cccacttgcc
    16201 aggaatggta gctgccaaaa caacccttcc tgtcataggg gtacctgtca agtctcgtgc
    16261 tcttagtgga gtggattcac tctattctat cgttcagatg ccgggtgggg tgcctgttgc
    16321 gaccatggct atcggtgaag ctggagcgac taacgcagct ctctttgccc tccgtctcct
    16381 ctctgtagaa gataagtcca ttgcggatgc acttgccaac tttgctgaag aacaaggaaa
    16441 aatcgcagag gagtcgtcaa atgagctcat ctaaaacaat cggaattatc ggtggcggtc
    16501 aactgggtca gatgatggcc atttctgcta tctacatggg ccacaaggtt atcgcgctgg
    16561 atcctgcggc ggattgcccg gtctctcgtg tggcggaaat cattgtggca ccttataacg
    16621 atgtagacgc cctccgtcag ttggcagacc gttgcgatgt cctcacttac gagtttgaaa
    16681 atgtcgacgc tgacggtttg gatgccgtta tcaaggatgg acaactccct caagggacag
    16741 acctgctccg catttcgcaa aatcgtattt ttgaaaagga ctttttgtca aacaaggctc
    16801 aagtcactgt ggcaccctac aaggtcgtga cttctagcct agacttggca gatatcgact
    16861 tgtcgaaaaa ctatgtcctc aagactgcga ctggtggcta cgatggtcat ggacaaaagg
    16921 ttattcgttc agaagtagac ttggaagcag cctatgcgct agcagaatca gcagactgcg
    16981 tcttggaaga atttgtcaac tttgcccttg agatttctgt catcgtgtca ggaaatggca
    17041 aggaggtgac gtttttccca gttcaggaaa atatccaccg caacaatatc ctgtctaaga
    17101 ccatcgtacc agcccgcatt tctgaaagtc tagtagacaa ggctaaagct atggcagtgc
    17161 gaatcgcaga acaactcaac ttgtctggaa ctctctgtgt ggaaatgttt gcgactgatg
    17221 atgacgttat tgtcaatgaa atcgccccac gaccacataa ctctgggcac tattctattg
    17281 aagcctgtga tttctctcag tttgacaccc atattctcgg tgttctcgga gcaccattac
    17341 cagtcatcaa actccatgcg ccagccgtta tgctcaatgt cctaggtcaa cacgtcgagg
    17401 ctgctgaaaa gtatgtcgca gaaaatccaa gcgcccacct ccacatgtat ggtaaaatag
    17461 aagcaaagca taatcgtaag atgggacatg tgaccttgtt tagtgatgta ccggatagtg
    17521 tggatgagct tggggaaggg attgattttt aggacaagtc tatgatacaa attatcgtta
    17581 atacatttat tgaaaaggat aagactggag cagttgttga agtgttgtat gccagtgctg
    17641 accaagataa ggtacaagct aaatatgaag aactagctgc acaatacccc gaaaattatt
    17701 tagctatcta tgatgtaccg ctggatacgg atttgaatac actagatcat tacccgtctg
    17761 tgtttattga aaaagaggag tttgagtaga aatcttggtt tacctagata gcttattccc
    17821 aacagcttaa gaagaaagga aaaattaaca catgatcaac cgttactctc gccctgagat
    17881 ggcgaatatt tggagtgaag aaaataaata ccgtgcttgg cttgaggtgg aaatcctctc
    17941 tgacgaggca tgggctgagt tgggggaaat ccctaaggaa gatgtggctt tgattcgcaa
    18001 gaaggcggac tttgacatcg accgtatttt ggaaattgag caggagacgc gccacgatgt
    18061 ggtggctttc acgcgtgcgg tttctgagac tcttggtgaa gagcgcaagt gggttcacta
    18121 tgggttaact tctaccgacg tggtggatac tgcttatggt tacctctaca agcaggccaa
    18181 cgacatcatc cgtcgtgacc ttgaaaactt cactaatatc atcgctgaca aggccaagga
    18241 gcacaagttc accatcatga tggggcgtac tcatggtgtg cacgctgagc cgacaacctt
    18301 tggtcttaaa ttggcaactt ggtacagcga aatgaaacgc aatatcgagc gtttcgagca
    18361 tgcagctgct ggtgtagaag ctggtaagat ttctggtgcg gttgggaact ttgccaatat
    18421 cccaccattt gtagaggagt atgtctgcga taaacttggc atccgtgccc aagaaatctc
    18481 tacacaagtc cttcctcgtg accttcacgc tgagtacttt gcggttcttg ccagcattgc
    18541 gacttcaatc gaacgtatgg cgactgagat tcgtggtcta caaaaatctg agcaacgcga
    18601 agtagaagag ttctttgcta aagggcaaaa agggtcttca gcaatgcctc acaaacgcaa
    18661 cccaatcggt tctgaaaata tgactggtct ggcgcgtgtc attcgtggtc acatgattac
    18721 ggcttatgaa aacgtcgctc tctggcacga acgcgatatt tctcactcat cagctgagcg
    18781 tatcatcaca ccagatacga ccattttgat tgactacatg ctcaaccgtt ttggaaatat
    18841 cgtcaagaac ttgacagtct tcccagaaaa tatgatccga aacatgaact cgacttttgg
    18901 tcttatcttt agccaacggg ctatgttgac attgattgaa aaaggcatga cccgtgagca
    18961 agcctatgac ttggtgcaac caaaaacagc ctactcttgg gacaaccaag tagactttaa
    19021 accacttctt gaggcagatt cagaagtaac atcacgtctc actcaagaag aaatcgatga
    19081 aatcttcaac ccagtttatt acaccaaacg agtggatgat atctttgaac gtcttggact
    19141 aggtgattaa ttaaaaaata aacagcgagc ttcaatctcg ctgtttattt tttatcgaaa
    19201 agacttagtc ttcttttctt ttagtgagtc cataggctgc tagtgtggac atgagtcctg
    19261 cgactactag tcctgcagaa tcgtgagttc ctgtttcagg aagttttttc tctgttacca
    19321 caggagctgg atcttgagga agagctttgc tttcctcagc aggagcagtt gatggagctg
    19381 gttggcttgg gatttctagt tttggttttt cttcagcaat agcggcttgt ccgttttcat
    19441 cgcctacatg tgttaccata gttccgactt cgactatttg agtaacggct tcctgtgcta
    19501 cgacactatt tacaagtgtt ttcacttcct taccatcggc agaagtgctc acagagtaga
    19561 agttgctacg atgtccattg acgcccttag taatgacttg tgtttttcct ttgagtaaga
    19621 gtggattttc acgagtcact gtggtaaatg gaatttcttc ttcttggata tccagtctag
    19681 gttttacctc agtagttggt gcaagaccac tttcatcacc cttgtgagtt acaggagagc
    19741 caacttcaac cacttggttt ataacttctt tggttacctg gctatcaagg actgtttctg
    19801 ttgtttttcc attttcagtg agtacagaga tgtaatgagt tcgttcacct ttgactcctg
    19861 ctgtgataat attttcctga ccagctggga ggttaggatt ttctttcttg ataacttcaa
    19921 atggaatttc ttcagttctt gtgatgagtt ctggtctggt ttcaacattg gcagccactt
    19981 cattttcatc taggcttcct gaatgagttg cagctggttt gaggccttga agagcggctt
    20041 ttaggttggc tacaagtgtg tcaagctcag cttgtttatt acggttgagg ttgtaattta
    20101 gagctgtttt agctgcgtca agggcctcaa gactttcttt actatatcct tctaagtttg
    20161 taggaatttt agctaattct tcgcggagag cattataatt agcacggaag tagtctttgt
    20221 tgtggtctgc aaaggcagtc atgagttcaa agatttcctc ttccttgtac tcagcgcttg
    20281 gtctatctgc ccagattgaa agcatacttc cgactgttgg aagatctact tcaggatatt
    20341 tggtagaagc tagttgattg aatggaactt taccagaatt ttcaactgct tttgaaagag
    20401 ggtaagcttc atcttgtttg tgattgccta taacataata ccagtcaccg ttggtattca
    20461 agaatttata gcctttgctt gctaggtatt gaggtgatgc gaggttatat ccccaccagc
    20521 ctttagacca gtaagaaatc aagacatctt tgtcaaactg aacatcgtcc ttgtcttcat
    20581 agtagaagcc atcgttgaag gccattggtt gaagccctct ttctttggcc atagctgcga
    20641 gggtgttggc atattcggca aatttgccat agagttgata ccacttgagg tagtaccagc
    20701 cttgggcact agtcgcatcg ttggcgtatt cgtcagtacc aaagttgaaa atctttgttt
    20761 tacctgcaaa gaagtccatg tatttaccga tgagggcttt tacaaagttc atcgcttctt
    20821 cgtttttcaa gtccatagtt gtttttgaaa ctttatcaaa gtgggcttga ggatttttaa
    20881 tacctaattt ttccatggca accagcatag catccatgtg acctggactg ttaatagctg
    20941 ggatgagacc aatgtcctta gatttagcgt attcaattag ctctgtcact tctgcctgtg
    21001 ttagtgcagt accgtttgga tcgtcgtagt aagctttagt tccttcgata atagcttttt
    21061 taacgtcatc actagcatag gtttttccgt tggcagtaat ggtcatatca ttgagtagaa
    21121 agcgaagtcc gtcatttcct agaaggagat ggacatcaga atatccgagc tcactggcct
    21181 tgtctacgat gcgtttgagc tggtccagag taaagtattt gcgtccagca tcgattgaga
    21241 ttaccttgtt tttggcaagt ttttcaacct cacgtttagc ttcttcttct ttttgagctt
    21301 caggcgtgag ggtcaagttg ttgacagttt cttgaagttt agcaatggct tgatcaatcg
    21361 tatcttgttg ggcacggcta aggttgctat cgagagagcg aatagctttt tcagcttctt
    21421 ttacggccgc gacgctttct gcagtataac ggttcaggtc ttttggtacc tcgttaagtg
    21481 cttgctctgc agattcataa tcagctgcga agtattcagc gttggcattt gcaaaatgac
    21541 gcatgagttt gaagaggtgt gatggtgaat aacgtgcaga tggagtgtca gcccaagcag
    21601 ctaccatacc accgatgatt gggatatcag ctccttctgt ttttggtaca gaagtgattg
    21661 gtgtgttttt aataccattg agcccctgat cgagattgta ccagccttgg ccatcagcgt
    21721 ttcgtccaag aacgtagtac caagcatcat tggtattaag gatttggtga cctttttcag
    21781 ctagtagttt agaagaagcg acatcgtagc ctccccaacc accagtccac atagaaacga
    21841 tgatgtcttt gtcaaaacta ccaaagcttg tgtcgctatt gtagtagata ccgtcgttaa
    21901 aggccattgg tttgagaccg tgcgatttta caatacgagc gaggtcattg gcgtaggcaa
    21961 taaatttttc atagcctttt acagggtagc cttcgtttgg atagtattta tcagcttgaa
    22021 gcacactcca acctttagca tctgtcgcat cattggcata ttcatcaagt ccgatgttga
    22081 agatttcagt ctttttcgcg aaataagcag catacttgtc gataagggct tttgtaaaag
    22141 cgacagcttg ttcgttgtca agatcgacag tacgggctga tttcttccca aaatagctaa
    22201 agttagggtt ttggattccc aattctttca tggcattgag aatcgcatcc atgtgtccag
    22261 gactatttac tgtcggaatg agaccgatac ctttatcttt ggcatagtta atcagatctg
    22321 tcatttgact ttctgttaag tgattgccgt ttggatcgtt gtaataatca tttgtacctt
    22381 tttcaatggc gcgtttgaca tcgtcactgt cataggtctt gccgttagct gtgatgctca
    22441 tatcgtccaa catgaaacgg agtccatcat ttccgactaa taggtgtaaa tcagtgtagc
    22501 cataatgttt cgctttatcg atgatttcct tgagctgttc tggtgagaaa tatttacgtc
    22561 cagcatcaat agaaacaatt ttctttttcg ctagtttttc atttacagtt gcagcacgtt
    22621 cctttcctgc ctctgtttcc ggtttgtcag cctctgcttt cgcttcatct tttttagctg
    22681 gtttatcttt gtcagtcttg tccgtatttg actctttaga atcaacctct ttcgcttctt
    22741 cctttttagg gctagcttct tctgcctttt tattagcagt ttctttttca gcagaagttg
    22801 gagttaccac ttctgcttta tcactaggag ttgaactaac ttcctcttgt ggtttttctt
    22861 ctgtttttgg aagactagct accttatcag tagctggagt ttctgtttct acagtttttg
    22921 gagcttctgg ttgaagctct gctttaggtg ttttctcagt ccgattttcg gatgattgag
    22981 gggaatcaga aaccgtatgg atggtcggtt ggttttctgt agtagtagga gtaactccat
    23041 cggctgcaac agtctgtgct tggaaggcaa atccaattag aacagaagct gctcctacag
    23101 cgtatttacg aatagaaaaa cgctgttgtt tttcatgttt cattgcaaaa cctcctgatt
    23161 gcattgttat attgatagcg attatataaa tcaacgcctt tattttattt cttatattaa
    23221 cgagagtcaa gaggagatga caaaaaacta taataagtat aaaaaaatat aaaatttaaa
    23281 cttaagattt cagattggtc gaaaaataca tatatatatc tagtataatt tttggttcta
    23341 tttctataaa atattccaca aattatagaa tcttccaaaa ataggtaagc gctacctttt
    23401 tggtgtggta taataagcat agaaaaagcc cgagcgatta ggctcaggct ttcttcttag
    23461 tgatcacggt cacatgagat gaatttaatc ttgtagtaat cagatcgttt gtaagtttca
    23521 ctgtattcta aaacttggcc agttgattcg agtttggtga ttttagtttg taggacagta
    23581 gggaattgtt catcgactcc gaggactgaa gccgcatgtt ctggagttgg aaagactatt
    23641 tcgttgattt cttcaaagtg ttcatcattc atgtgaatgt ggtagtctaa cttgaaacga
    23701 ttatagatag aactatagta ttcaaggttt ggataatttg cgttgatata ttgttctggg
    23761 atgtaggatg tatggtagat ataaacgaca ccgtttgatt cgcggatacg ttcaatcttg
    23821 tagtagaatt gatcgccgcg tagacccaat ttttccaagt aaacaagctt gtttccgcgt
    23881 tcaattgaaa gaacagttac cttatcatct ttagcattga agagctcaat atctgaaaac
    23941 tctacaagct tgtgtttgcg tgcacgtgaa acgaaggttc cttttccttg ttggcggaca
    24001 atatagccat ctttggcaag gtcgtttaag gcgcgaacaa ctgtgataga gctgacatcg
    24061 tacattgaaa tgagttctgc ttcagtgtaa aatttatctc cactgctaaa ctgcccagag
    24121 atgattttat tttttaattc gtcttttatg tattgatatt tgggaattgc catattttta
    24181 cctctatttt ccttctccat agaaaatttt accataaaag cgaaaaaaat agtagtcttt
    24241 tgaataaaaa attagaataa tagt
//
LOCUS       DAVKHZ010000024        24076 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000024 DAVKHZ010000000
VERSION     DAVKHZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.024"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            1..1076
                     /locus_tag="VWW99_000896"
                     /pseudo
     CDS             1..1076
                     /locus_tag="VWW99_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1175..2797)
                     /gene="groL"
                     /locus_tag="VWW99_000897"
     CDS             complement(1175..2797)
                     /gene="groL"
                     /locus_tag="VWW99_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9538762.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2813..3097)
                     /gene="groES"
                     /locus_tag="VWW99_000898"
     CDS             complement(2813..3097)
                     /gene="groES"
                     /locus_tag="VWW99_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9538763.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3253..3648)
                     /locus_tag="VWW99_000899"
     CDS             complement(3253..3648)
                     /locus_tag="VWW99_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9538764.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3726..4487)
                     /locus_tag="VWW99_000900"
     CDS             complement(3726..4487)
                     /locus_tag="VWW99_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9538765.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(4529..5155)
                     /locus_tag="VWW99_000901"
     CDS             complement(4529..5155)
                     /locus_tag="VWW99_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9538766.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(5171..5488)
                     /locus_tag="VWW99_000902"
     CDS             complement(5171..5488)
                     /locus_tag="VWW99_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9538767.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5485..5784)
                     /locus_tag="VWW99_000903"
     CDS             complement(5485..5784)
                     /locus_tag="VWW99_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9538768.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(6069..6260)
                     /locus_tag="VWW99_000904"
     CDS             complement(6069..6260)
                     /locus_tag="VWW99_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9538769.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6421..7095)
                     /locus_tag="VWW99_000905"
     CDS             complement(6421..7095)
                     /locus_tag="VWW99_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538770.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(7101..7547)
                     /locus_tag="VWW99_000906"
     CDS             complement(7101..7547)
                     /locus_tag="VWW99_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538771.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7661..8164)
                     /locus_tag="VWW99_000907"
     CDS             complement(7661..8164)
                     /locus_tag="VWW99_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9538772.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8161..8523)
                     /locus_tag="VWW99_000908"
     CDS             complement(8161..8523)
                     /locus_tag="VWW99_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9538773.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8589..10454)
                     /locus_tag="VWW99_000909"
                     /pseudo
     CDS             complement(8589..10454)
                     /locus_tag="VWW99_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10486..10935)
                     /locus_tag="VWW99_000910"
     CDS             complement(10486..10935)
                     /locus_tag="VWW99_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9538774.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(11059..11775)
                     /locus_tag="VWW99_000911"
     CDS             complement(11059..11775)
                     /locus_tag="VWW99_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9538775.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12658..14073)
                     /gene="ply"
                     /locus_tag="VWW99_000912"
     CDS             complement(12658..14073)
                     /gene="ply"
                     /locus_tag="VWW99_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9538776.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGVRVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14084..14416)
                     /locus_tag="VWW99_000913"
     CDS             complement(14084..14416)
                     /locus_tag="VWW99_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538777.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14487..15095)
                     /locus_tag="VWW99_000914"
     CDS             complement(14487..15095)
                     /locus_tag="VWW99_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538778.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(15108..15566)
                     /locus_tag="VWW99_000915"
     CDS             complement(15108..15566)
                     /locus_tag="VWW99_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9538779.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15691..16493
                     /locus_tag="VWW99_000916"
     CDS             join(15691..16141,16141..16493)
                     /locus_tag="VWW99_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9538780.1"
                     /translation="MNYEASKQLTDAQFKRLVSVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLNLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANYSGKKKCHAMKAQAIVTSQGRFVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(17257..17517)
                     /locus_tag="VWW99_000917"
     CDS             complement(17257..17517)
                     /locus_tag="VWW99_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538781.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(17576..18127)
                     /locus_tag="VWW99_000918"
     CDS             complement(17576..18127)
                     /locus_tag="VWW99_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538782.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFHSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(18117..18314)
                     /locus_tag="VWW99_000919"
     CDS             complement(18117..18314)
                     /locus_tag="VWW99_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538783.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDSISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(18318..19112)
                     /locus_tag="VWW99_000920"
     CDS             complement(18318..19112)
                     /locus_tag="VWW99_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538784.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLTKGQKVVNVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(19520..19945)
                     /locus_tag="VWW99_000921"
     CDS             complement(19520..19945)
                     /locus_tag="VWW99_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538785.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(20074..20457)
                     /locus_tag="VWW99_000922"
     CDS             complement(20074..20457)
                     /locus_tag="VWW99_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538786.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKEMASG"
     gene            complement(20479..20685)
                     /locus_tag="VWW99_000923"
     CDS             complement(20479..20685)
                     /locus_tag="VWW99_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538787.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(20672..20833)
                     /locus_tag="VWW99_000924"
     CDS             complement(20672..20833)
                     /locus_tag="VWW99_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9538788.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(21192..22148)
                     /gene="lytA"
                     /locus_tag="VWW99_000925"
     CDS             complement(21192..22148)
                     /gene="lytA"
                     /locus_tag="VWW99_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9538789.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(22524..23894)
                     /locus_tag="VWW99_000926"
     CDS             complement(22524..23894)
                     /locus_tag="VWW99_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9538790.1"
                     /translation="MNKKRTVDLIHGPILLSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQSLGAKRPDRIAQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         7292 a         5340 c         4172 g         7272 t
ORIGIN      
        1 cgtcgattca agtgttatca ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag
       61 aagaatcacc aaatccctcg tattatcaac caaaaaattg cgcaaaagtt aattgaaaag
      121 atttctatga ccgatattgc ccatcagctt tccatctcaa cttcaactgt tattcgtaag
      181 ctcaatgatt ttcactttaa acatgatttt tcttgtcttc ctgagattat gtcttgggat
      241 gagtatgctt ttacaaaggg aaagatgagt ttcattgcgc aagattttaa caatctcaat
      301 attatcactg ttcttgaagg cagaacacaa gctgtcatcc gaaatcactt tcttcgctac
      361 gatagagccg tccgatgtcg cgtcaaaatc attactatgg atatgtttag cccttactat
      421 gacttggcta aacagcttcg ctttcaaatt tctaggctca ggctgaaaca gtctcccaga
      481 ctgtttcact cccgaatgct aaaatcgttc ttgatcgctt tcacattgta caacatctta
      541 gccgtgctat gagtcgtgtg catgtccaaa tcatgaatca gtttcatcga aaatcccatg
      601 aatacaaggc tatcaagcgc tactggaaac tcattcaaca ggatagccgt aaactgagtg
      661 ataagcgatt ttatcgtcct acttttcgta tgcacttaac caataaagag attttaaaca
      721 agcttttgag ctattcggaa gacttgaaac accactatca gctctatcag ctcttgcttt
      781 ttcacttcca gaacaaggaa ccagagaaat ttttcggact tattgaggac aatcttaagc
      841 aggttcatcc tctttttcag actgtcttta aaaccttcct caaagataaa gaaaagattg
      901 tcaacgccct tcaactacac tattctaatg ccaaactgga agcgaccaat aatctcatca
      961 aacttatcaa gcgcaatgcc tttggttttc ggaactttga aaacttcaaa aaacggattt
     1021 ttatcgccct gaatatcaaa aaagaaagga cgaaatttgt cctttctcga gcttagctga
     1081 cttcaaccca ctacagttga caaagagcct ataaaaagaa gggttagtca ttccctcctt
     1141 ttgtgttttt tataagttgt tttctataga aagcttacat catcccgccc atcatacttg
     1201 gatccattgc tggagctggg gctactggtt ctggtttatt ggctacgact gcttctgttg
     1261 tcaaaatcaa gctggctaca gatgctgcat tttgtagggc tgaacgactc actttaactg
     1321 gatcaatgat accttgatca atcatgttaa cccactcgcc agttgctgcg ttaaatccta
     1381 taccaagctc agcatttttc aaacgatcga taacgataga tccttcaaat cctgcattgt
     1441 gagcaatttg acgaacgggt tcttccaaag cacggagaac aatattacgt cctgttgctt
     1501 catctcctgt caattccaag gtagcaacag ctggaatcac attggcaaga gctgttccac
     1561 cgcctgcaac aataccttct tcaacagctg cacgagtagc gttgagggca tcttcaatgc
     1621 ggagtttcat ttctttcaac tcagtttcag ttgcggctcc gaccttaata accgctacac
     1681 cacctgacaa tttggccaag cgttcttgca atttttcacg gtcaaattca gaagttgtag
     1741 tttcgatttg agacttgata accgcaacac ggtgagaaat cgcttcagga tttcctgcac
     1801 cttctacaat aaccgtgcta tctttgtcca cggtcactct cgctgcttga ccaagagctt
     1861 caattgtcgc atctttcaac tcaagaccaa ggtcttctgt gataactgtt ccgcctgtta
     1921 agatggcgat atcttcaagc atggctttgc gacggtcacc aaaaccaggt gccttgactg
     1981 ctactacgtt gaaggttcca cgaatcttgt tcaaaacaag agttggaaga gcctcgccat
     2041 ccacatcatc cgcaataatc aagagtggac gattgctttg gagaatgctt tccaaaagtg
     2101 gcaagatctc ttggatattg gaaattttct tgtctgtaat caaaatgtac ggattttcaa
     2161 ggtcagccac cattttttcg ctatctgtca ccatgtactg tgaaaggtaa ccacggtcaa
     2221 actgcattcc ttctacgact tcaagctctg tttccatacc acgtgactct tcgatggtga
     2281 tgacaccgtc tttgccaact ttttccattg cttcagagat gtactcacca actttttcag
     2341 aacgagaaga tacggctgca acttgagcga tagcttcttt attggcaaca gggatggcgt
     2401 tgtttttcaa agcttcaact gctgcggcaa ctgctgtttc aatcccacga cgaataccga
     2461 ttggatttgc acctgctgtg acgtttttga ttccttcacg gacgatagct tgggtcaaga
     2521 ctgttgcagt cgtagtcccg tcacctgcga tatcattggt tttagaagct acttctgata
     2581 ccaatttggc acccatattt tcaaaatggt cttctaattc aatttctttg gcaatagtca
     2641 caccgtcatt ggtaatcaat ggtgagccaa atgatttttc aagaacgaca ttgcgacctt
     2701 ttggtcccaa ggttacttta acagtatctg caaggatatc gacaccacgg accatagctg
     2761 aacgggcatc tgatgaaaat ttaatttctt ttgacatact tactttctcc ttctattctt
     2821 caatgattgc caagatgtta gcttcgccta cgatgatata cttttcatcg ccatctttga
     2881 catcaagacc tgcgtgggct tcaactaaga cacggtctcc agttttaaca cttggagcaa
     2941 ccaagtcacc gttcaaggta cgaacacctt gtccagtagc cacaacttgg gctgttttgg
     3001 ttttttcttg ggctgaacct gcgagaacaa agcctccaac agtttgttct ttttcttcta
     3061 cttttaagac caaacggtcc cctaatggtt tcaacatctg atttcctcca taatgagata
     3121 gatattatta gcactcttta tacctgagtg ctaattcata gttctattgt atcacttagt
     3181 cagaaataat caagaaaaaa gtctgacttt ctcaagataa aaagcctgag accaactcag
     3241 actttttaat tcttaaaatg gcaattcttc ctcttccaag accaaatctg ccaaatcttg
     3301 gcctgcatta ttttcacgca tagcacgttg ggcacgactt tccaagagtt ggaatcctgt
     3361 cacaaggact tcggttacat aattcatctg accatttttc tcaaagcgac gggtacgcaa
     3421 ttctccatca acggaaatga gactaccttt ggttgcgtag cttgccaaag tttctgctag
     3481 tctgccccat aggaccatat tgacaaaatc aacttcacgt tcaccgtttt ggtctttgta
     3541 acgacggttg acagcgatag ttgctcgcgc tactgacttg tcattgttgg ttttgtgcaa
     3601 ttctggtgta gacgttaaac gtccaatcaa gataacttta ttatacatat tttcttcctc
     3661 ctacttatct attcgtagga aatcaaaaaa agttacagaa atttgtaact tttcgagaaa
     3721 attttttatt ttttatgaac catgaaacct gtcgcctgtt gattggccat aatggtcata
     3781 tctgtaatct gaacacgacg aggttgacta gtcacataga ctactgtatc tgcaatatcc
     3841 tgagcttgca aagcttctat tccttggtaa acggacgcag ctcgttcttt atcaccatga
     3901 aaacgcactg tagaaaaatc tgtttcgaca attccaggct gaatggttgt caccttgata
     3961 tccgttgcga tggtatcaat tcgcagtcca tccgaaaagg tcttaaccgc agccttggta
     4021 gctgagtaaa cagcggcacc agcataggcg tagattcctg cggttgaccc catattgata
     4081 atatgacctt gattgacttt tatcattgct ggcaagaaac agcgagtaac cgccatcaga
     4141 cctttaacat tggtatccaa catggtcaac atatccaact cctcatagtc ttgataggga
     4201 gccaagtcaa gagccagtcc ggcgttgttg accaagatat caatcctccc tatcgtttct
     4261 agaatatcag agcaaacagt cttcaccatg accatatccg tcacatctag tggaaaagtc
     4321 cagacggttt gatttggaaa gcttactgca aactccgact tgaggatttc taatctgtct
     4381 atccgtcgtc ctgttagaac gacatcctca ccctgctcca gataagcacg cgcaatcgct
     4441 tcaccgattc ctgaggttgc tcccgtaatc acaacatttt ttgccatctt atttccttct
     4501 agctggtcta tcagatatta acaacttctt aggcagtcca gtgtttagct gagtcaaatg
     4561 gtgttccgac aacttggtct tctgataatt caagcacacc acgtttttgt ggagcatttg
     4621 gcagatgcaa ttcacgagga ctgcacatca taccaaaact cttttcacca cgaagttcac
     4681 ctgggaaaat gagattccct tttggcatca tagctccagg aagcgcgaca atggttttca
     4741 accccacacg cgcattggga gctcctgcaa cgatttgtac agtcttatca cttgcgactg
     4801 caacttggca gatgttgagg tggtcactat ctggatgggc taccatctca acaatttcac
     4861 cgacaacaaa cttaggttcc ttatcattaa caatttcttc tgtaaaacct tccgcctgca
     4921 actcttggtt caaacgagcg acttgctcat ctgtcaaaaa gacttgaccg cgctctgcag
     4981 tttcaaataa acttgaaact tcgaaaatat tccaagccac tgtttcccca ttttctttga
     5041 ggaaaacacg ggctaccttg cctttgcgtt ccacattcag tttggcatct ccgctatttt
     5101 tcacgatgac cataaggaca tcaccgacat gttctttgtt atacgtaaaa atcattgttt
     5161 cctttttctc ctatttcaat cctgctaaaa aatcgttaat ttgctcctta cttttacggt
     5221 cgcgattgac aaaacgaccg atttccttgt ccttttctag aacaacaagg ctaggaattc
     5281 cgtaaacatc ccagagtttg gccaaatcca tatactgatc tcggtccatt cgaataaagg
     5341 tgaactctgg attggtctct tcaatctctg gtaaggcagg ataaatataa cgacaatcgc
     5401 cacaccagtc tgccacaaaa aggaagacct tcttgcccgc tttttccact aaagatgcta
     5461 attcttctaa acttgctggc tgtatcataa gacttcctcc tcatagacta ggtcttcatt
     5521 ttcatagaca aaggtataat gacggccatc ctcaaaaatg acgccaccaa ccaagctctc
     5581 cagactgctt tcgtaaactt gaacataaag ggtcgcaatt tcccccatgt cggaaaaatg
     5641 gtctcgcaca atctctgtca actcttcctg agtcttcatg agcttacggt catctgcaac
     5701 ttttttcgta gcaagagcaa ggcttccgat acctagcaga gccaagcctg ccatccacat
     5761 ttttttagct ttcataccat tcattttaac acaaaaaagg cttcaggaca aatgaggaag
     5821 cagcagaaaa gcaagtaaaa agcctcttcc tttaaggaaa aggacttctt atactaagag
     5881 caaactagaa agctagccgc aggctgctca aaacacggtt ttgaggttgt agataaggcg
     5941 acgttgacgt ggtttgaaga gattttcaaa gagtattatt cttattgcca ggcacctaag
     6001 ttgccaacgt agtaaccatc aggtgtgtag gtattgcgaa gcatcttacc tgatgaagcc
     6061 agataatact acttgccatt gtctttgacc caatcattcg caatcatgga accagaagaa
     6121 cttacataat accattctcc cttgtcataa acccaagtac tgactttcat ggttcctgag
     6181 caattaaagg caaaaaaact gtccaatatc attcgttttt taaaagcatt cgacactaca
     6241 tcatctacgc tgtagtacat agatacatca aacgtaaaag ccaaaatatc gaaaaatata
     6301 caacttttaa aggcagcatt tcatttttta aagtctaaca accatagaac tgaaatcaga
     6361 gcaaggcatc aatttcctac tctatagtac gcctattttg atggttgaag tgaagatttt
     6421 ttatttaacg tagacaattt ccaacttccc aaaagccaca tctccacgga taatcaaggt
     6481 tttgctggtt ggggctacag gagcgtcagc cttagctgca ccaaaggagg tttctacttt
     6541 caaatctaca cgccagtgtt gtggaacata gacggttgca tttccgaagg ctacttcaat
     6601 atttaaagtt gcaaaatcac ctagcatctc tgcattatca tagtagattt tagcatcccc
     6661 aaaagcgact tccacttggt catctacgag atcttgatct tgcttataga aggtcccgct
     6721 accaaaagcg acttccttat ccgtgacgac tgtttttttc ccattgtacc accatttttt
     6781 ttcattccag aacttacttg aatgcgtcag ataaccaaca ccaagtacca ccaagatgct
     6841 agcccaaata agagaatgat tggtaactgg taacaagtca taagcgtaat ttgcaatgat
     6901 gagcgccagt aaacctgtaa aaactgccga agtgagatga cgtctaagga tggactcaat
     6961 ggacttataa gcaaaaaaaa caatacctag taaaggccat attttaccat ccaaagaagg
     7021 aattccaaaa ttcccttgca gcaagatcca agctgctaaa accaataaaa caataccaaa
     7081 tgctttcttt ttcatgtttt ttacctcatg tttcttagat tttcttttag gagggattgg
     7141 taatgccgtg agacatgaac ctccttgtgc gtctgataaa aggaaatggt gcccgttcct
     7201 gaaaaggact tgtccactga gtaaatctga cggatgtttg cgatcgttga cttggaaact
     7261 cgattaaaat agcgaggcaa gatagactcc aactcataga gcttgaggcg aacctcataa
     7321 gcttccttct ggttatgagc gtagatcttg ctcccttctg tttcaaagaa gagaatttct
     7381 gacaggtcta gataatattc acctgtcccc ttgtaaaaag tcaacctcgg agcctttgac
     7441 tcttgcaaga gccgttgcaa gtctgcaatt tcatctgtca aagtgggggt cttgatgaca
     7501 atttcagtct cctctaaatt gccgtcaatc tcaattcgta acttcatcac atctcctttc
     7561 tgactctact atatcaaagc taagttagga atacaagggc aaaaaagcaa gtggtcaatt
     7621 tgaagcagta agtggttgta ggactagctt aacttacatg tcagaatagg aagaaaagga
     7681 atccccacac caaaccacaa gcatagccaa ccaagacatc cttgggataa tgaacacccc
     7741 ctaaaaccct caccaacccc agcaaagctg aaaagatcaa gcatactaag ccaataggta
     7801 gactagcatg cagacaggcc atggagatga tagttgccga aaagacatgg cgactgggca
     7861 tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat atcccaagtt tcataaggtc
     7921 tcgggtgatt gattttctta cgaaacaggg acaaggtcac aaaacctgat gcaggaataa
     7981 gcacataaac cccaacttgc tgaccaagtc ctagttgcag gtaagtggtg actagcaaga
     8041 tcagatagac caggggcatg gcgactgtca tcaaacgatt gaaactgcgt aataggaata
     8101 aaagaattgg acgactagtt agtctggagc tgatatttcg ataccattct tgataatttt
     8161 tcatttatgc ttctcatact tttctagaac ttgttgagat gttttcccct tggcaagcca
     8221 atccactagc ttgtccaagc gacgtatttc ctgcatcaga ggatcatcaa tttcctcaat
     8281 acggacaccg caaatcttcc ctgtaattgc aattcgttca gggacataat atggtgcttg
     8341 tctaaaaaaa tctccgtaag taacatccct atccagacaa gcaagaacat cactcacttc
     8401 ataccctgtc aaccaactgg tcacttggtg aactgactct gcctttccac cctttcgctc
     8461 aacttttgca atcaaggcta agtagacagc agcaaatttc atattgtata atttctgact
     8521 catacttctc tctttctatg tgtactagta taaataagaa aaagaaggcc gtcaagcctt
     8581 cttttgattt attcttctgc ttcatcttct gtaaattgac tattgtacaa gtcagcgtag
     8641 aagccacctt gcgccatcag ttcctcatag ttgccttgct cgatgatatt tccatctttc
     8701 atgaccaaga tcaagtctgc atttcggatg gttgacaagc ggtgggcaat gacaaaggat
     8761 gtgcgtcatt ccatcaaacg gtccatggct ttttggatca attcctctgt ccgtgtgtca
     8821 acagaagaag tcgcctcatc caaaatcaaa agcggtgcat ccttaagaag ggcacgagca
     8881 atagtcaata gttgtttttg tcctacagac aaggtcacgg tgtcatccaa gatggtatca
     8941 tagccatctg gcaaggtcat aataaagtgg tgaattccca cagccttact agcttccatc
     9001 attcgttcat cactaatccc tatttgatta tagatgagat tgtctcgaat agttccttca
     9061 aagagccagg tatcctgcaa gaccattgaa aaggcatcat gcacttctga acgcgtcata
     9121 gccttggtat ccacaccatc aatgcgaata cttcccttat caatctcata gaatttcatc
     9181 aaaagattga caatggttgt cttaccagcc ccagtcggcc caacaatggc aaccttttga
     9241 ccagcatgag ctgtcgcaga gaagtcatag tcttgaacat tgacaccgtc caccagaatt
     9301 tctcctgctg acacgtcgta gaaacgtgga atcagattga ccagagttga tttaccagaa
     9361 cctgttgacc caataaaggc cactgtttga ccagtttctg ctttaaagct aacatgttca
     9421 ataactgcct ccgaatttgc cgcatagcgg aaggtcacat ccttaaactc gacctgacct
     9481 ttgaagtttt catcagtcag ctgcacttga acagggtttt ggatagaaga atgcaaatct
     9541 aaaacttgat taatccgctt agcagagacc atagttcggg gaagaacgat gaagagtgct
     9601 cccatgagaa ggaagcccat gacaacctac atggcataag acatgaaaac aatcatgtca
     9661 ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa tcacataggc cccaatccag
     9721 taaatcgcta cactcaaacc acttgaaatc cccatcatga taggattcaa aatagccata
     9781 agacggttga caaacaaatt caaacgggtc aattcatcat ttactgctgc aaatttttca
     9841 ttttgataat cctctgcatt gtaggcacga acgacacgaa tacctgttaa actctcacga
     9901 gtgatactgt tcagtttatc tatcagcccc tgaatcaagg actgttttgg aaagtctagc
     9961 gtcatcaaaa cggtcgtcat caggacgttg acaatcactg ccacaagtac ggcccaaagc
    10021 cagtattctg aatgacctaa aatcttccca atagcccaaa tagccataat tgaaccacgc
    10081 gttaccactt gcaagcccat agtaatcaac atttgaactt gagtaatgtc attggtagta
    10141 cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg tctgcgagta atccaaaact
    10201 cggttaaaaa tatcacttct cagcctacta gtataagaag ccgccactcg ggatgcaaaa
    10261 aatccaactg caactacgga caagaaggca agaaagagca ttcccatcat catgcttgcc
    10321 ggctgccaca actcatctaa attagttcct tgactaccta gcaaatccgt aattttcgag
    10381 atataggtcg gcacttccaa ctctagatag accgaaaagc aagtaaagag aatggctagt
    10441 aaaatcatcc ccattctttt ctactaattc ttttggctaa tttctttatt ctctcctcct
    10501 attcccttga tattttgcct gtagttgacc gagaaccttc tcaaaaatca gtaattcatc
    10561 ttcatcaatg tcttccatca actgcttgtc tatgcgttca aaaaaagcct taacctgttg
    10621 catctgagaa cgtgctttgt ccgtcagacg aacaaactta gcccgcttat caacaggact
    10681 cgcctccaat tccaccaaac cattttgcac tatacgctta accagattac tagcaacaga
    10741 cttggtaata ttgagttcct gatcgatatc tttaatcaag accaagtctt ggtttttctc
    10801 gcgattatcc aaaaaatgca caacctgacc ttgcggccca cccataaatt caatgccgca
    10861 acgtttggct tccttttgca ccatcaggtg aatttgatga ccaaaacgct taaagactaa
    10921 catcggttta cccataatct cccccttcta aataaaaata gttctctgga gaataattaa
    10981 atttctatga gaactatttt cttgattaaa aaaatcccaa gtgattttct cacttaggat
    11041 catgttctat aggttaaatt aaaacccatc tacgttcgta taaatctttt ggacgtcttc
    11101 gtcgtcttca agaacgctgt aaagtttttc aaaggtttca aggtcttcgc ctgacaattc
    11161 cacttctgac tgaggaatca tttccaattc agtcacttgg aattcttcaa taccagactc
    11221 acggagggca acgatagcct tgtgaaggtc agttggcgct gtgtaaactg tgattgtacc
    11281 ttcttgtgct tctacgtcat ccacatccac atccgcttcg agcaattgct caaagactgc
    11341 gtccgcatct tcacctccaa atacaataac acctttgttg tcaaagaggt aagaaacaga
    11401 acctgaagcg cccatgtttc cgccgttttt accaaaggct gcacggacat tggctgctgt
    11461 acggttgacg ttagaagtca aagtatccac aattagcata gagccatttg gcccaaaacc
    11521 ttcgtaacgt ccttctgtaa aggtttcgtc tgtgtttcct ttggctttat caatcgcttt
    11581 atcgataatg tgttttggca cttgggcttg tttagcacgg tcgataacga atttcaaagc
    11641 tgagtttgat tctggatctg gatcaccttt tttagctgct acatagattt ctacaccaaa
    11701 ttttgcatat actttagagt tggctccatc tttagccgtt ttcttggcta cgatattggc
    11761 ccatttacgt cccattagga atctcctttt ttcacatttt aatctttctt attataacac
    11821 aagttttttt gattttcact agaggaaatg gattttatta gcaaatcaag ctaggatagc
    11881 actttacctg ctaagatggt cttgcctttc tatcttttta tcaacagaca ctcatccaca
    11941 ttcaaaaaac aaactagacc attatctgca aatagaaagt ttcagccaag tttgacaaag
    12001 tcagctcaaa ttactgtttg aagtttgtag atataagcga caaaaacaat catactgcac
    12061 cttttgttga cagtctactc cagacatatc atagttcaag taaatacttt gaaatccaac
    12121 agttcttata ggcgctattg tattccaaga aatcaataga agagtttcta agcaaacctc
    12181 taatactcaa taaaaatcaa aaagcaaact agaaagctag ccgcaggttg ctcaaaacac
    12241 tgttttgagg ttgcggatag ggctgacctg gtttgaagag attttcgaag agtataattt
    12301 acgtgttccc aagatggaga aattagacta gtacactggc acttctaaac cattgctagc
    12361 aattgatttg ttcatattta atttcatttt ttccataaat gggtattaga tataaacagc
    12421 aaaatatttc cgatacgtgt cattcttgaa tttccaatca tctaaaacaa gtaaaggata
    12481 atcaatcccc tgtatatcaa ggaattggct acccttttta cttttttaca cattctgttt
    12541 gatagattca ttttaacatc acgagcatac tccaatggaa atcgctaggc aagagataaa
    12601 ctttcagata tccgcagaga gatcatcgcc tctttttgtc gcaagcattc tcctctccta
    12661 gtcattttct accttatctt ctacctgagg atagagagtt gttccccaaa tagaaatcgt
    12721 ccgcttacgc actagtggca aatcggtttt ttcataaacc gtacgccacc attcccaggc
    12781 aagcccggta cactctctaa ttttgacaga gagattacga acattccctt ttaaaggaat
    12841 actagtggta aagtgagccg tcaaatcctg cccatttctg tcccaagcct taggagtcaa
    12901 gacttcctta ccttgatgat cataggataa ttcatcccaa gtaatataat attgggcaac
    12961 ataggcacca ctatgatcca gcagtaaatc tccgtttctg taagctgtaa ccttagtctc
    13021 aacatagtct gtactatttt gaaaggtcgc aactacattg tcacgtaaaa aagaagttgt
    13081 ataggaaatc ggcaagcctg gatgatctgc tgtaaagcga ctgccttctt gaatcaagtc
    13141 ctctaccatg tccaccttgc ctgttacaac tcggacaccc gaacttgggt cgccccctaa
    13201 aataaccgcc ttcacttctg tattgtccaa aatctgcttc cactctgtct gaggagctac
    13261 cttgactcct tttatcaaag cttcaaaagc agcctctact tcatcactct tactcgtggt
    13321 ttccaacttg agatagactt ggcgcccata agcaacactc gaaatataga ccaaaggacg
    13381 ctctgcagaa attcctctct gttttaaatc ctctaccgtt acagtatctt gaaacacatc
    13441 tcctggattt ttaacagcat ctacgctgac tgtataataa atctgcttaa aattaacaat
    13501 ctgaatctgc ttttcgcctg aatggacaga gttaaaatca atatcaagag aattccctgt
    13561 cttttcaaag tcagaaccaa acttgacctt gagttgttcc atgctgtgag ccgtgatttt
    13621 ttcatactgc attctagctg ggacattatt gacctgacca taatcttgat gccacttagc
    13681 caacaaatcg tttaccgctc cgcgaacact tgaattgctg gggtcttcca cttggagaaa
    13741 gctatcgcta cttgccaaac caggcaaatc aatactataa gtcatcggag cacgatcgac
    13801 cgcaagaaga gtgggattat tctctaacaa ggtctcatcc actacgagaa gtgctccagg
    13861 atagaggcga ctgtcgttgg tagctgttac agaaatatca cttgtatttg tcgacaagct
    13921 ccgcttcttt ctttcgataa caacaaactc atcgggtagc tgattaccct ctttgatgaa
    13981 acgattttca atactttctc cctgatgggt caagagtttc tttttatcgt aattcatagc
    14041 tagtataaag tcatttactg ctttatttgc catcttctac ctcctaataa gttcctggat
    14101 tgagttgcat aaactcagac ttgttcagcg aaatcagccg tggttggact aagtgatcca
    14161 aaatttcctc gtacaattct tctgagacat tgcgtcgccg tctggctaga taagaagtcg
    14221 gaataaccgt attatccaac ataaatacct tatctaagtc aatcaaggtt ggtcttgtaa
    14281 aaggattacg agctagatcc ggctcttcta tcataaagtt cttgaccaaa cgtctggtca
    14341 agagagctgg tttgaaggtc tgatttttaa ccaactcttt gtttttagtc atgctgttgt
    14401 caatacagat atacatatga ttcttcacag ccaaatcgct actaatagtc ggaaaaggca
    14461 aataaagaac tacaacatct cctctcttaa tcaagcaaga gcaccccctt ttctcctaat
    14521 gtaacataga caggattgac caagtcttct gattgactca gaatttccaa agtttgagtt
    14581 tggcgcgctg tcaatttagt agcatcttgt ctcttcaata caaaatgctt gtcgccaata
    14641 accttgacaa tataatcctt ctccaaagct gactggtaaa tccacatcag atgttgtctg
    14701 tcctgagaac tcaagagaga aggattttca agcctcccga tagtctgata aaaatcaaaa
    14761 acaggagcta actcctgcca atctgattgg ctagttgtca aggctagaaa aagggctttg
    14821 cgagctgata cttcttggtt agccttgaga gttactttcc cctccaagtt ttttagaaat
    14881 cgggaaactc cagaaagcaa atttttctct aactgcgaga aataaaaacc tttcgttccc
    14941 agacataagt ctttcatgtc gctttctcta gcaaataaga gctcaaacat ttgatagtaa
    15001 aagaaaaata tctggcactg ggtcgcgctc atcttttcct tatcggcttc tttttttaac
    15061 cagagcaagg gcgacaggta gctggattga gacatttcct ctacctccta ctctttttta
    15121 actggagcat ctgcactagc tgccacttct tttgactgga tactttccca ctggttaatc
    15181 tcctctgaga taagaccttc gcatgtcttg acaaataggg caaaagcctt ggtctttcct
    15241 gcatatttct ccgtttggca ttgatagagg aatttttctt tctccaggag ttgcgcagtt
    15301 ttttggtaag aaatccaatt ttcctttgca ttatacaaat tgataatccc ctcacacagc
    15361 aagccgagac tggataaggc aaccgaaatc aaacggtagc gatcacctgg cataggaata
    15421 gcacaaaaga cagctatgag gaaacctgcc acgatttctg ttatttttaa taccttatag
    15481 cgcctacgat gttgaacgct tttctttaaa aaatgagcta tctgtacgtc taatcgctct
    15541 gtcaggtaca tttcttctgg cgtcatattc gtaactcctt tcatttactt tgataatcat
    15601 agtataacat atttgaatat caaagtatag taaaaactgg actataactt attagacttc
    15661 ctacgaaaca aaatatggta tagtagttct atgaattatg aagcaagtaa acaactaact
    15721 gatgcacaat ttaagcgtct tgttagtgtt cagcgtacca cttttgaaga gatgttagct
    15781 gtattaaaaa cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaaac
    15841 ctagaagacc ttcttatggc cactcttcaa tatgtgcgag aatatcgaac ttatgaacaa
    15901 attgcggctg attttggtat tcacgaaagc aacttaatcc gtcggagcca atgggttgaa
    15961 gtaactcttg ttcaaagtgg tgttacgatt tcaagaactc ctctcagttc tgaggacacg
    16021 gtaatgattg atgcgacgga agtacaaatc aatcgcccta aaaaagaatt agcgaattat
    16081 tctggtaaaa agaaatgcca cgctatgaag gctcaagcga ttgtcacaag tcaagggaga
    16141 ttgtttcttt ggatatcact gtgaactatt gtcatgatat gaagttgttc aaaatgagtc
    16201 gcagaaatat cggacaagct ggtaaaatct tggctgacag tggttatcaa gggctcatga
    16261 agatatatcc tcaagcacaa actccacgta aatccagcaa actcaagccg ctaacagctg
    16321 aagataaagc ctgtaaccat gcgctatcca aggagagaag caaggttgag aacatctttg
    16381 ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt aaacgcttcg
    16441 gattacgaat gaatttgatt gctggtatta tcaatcatga actaggattc tagttttgca
    16501 ggaagtctat tatatatatg cacagacata tgaaatcttt ttgatacatc cttcttttta
    16561 catagagaga cagcagttat gatatattta tccatacaca acatcttctg taccaatact
    16621 acaatattgg tacattttga ttacacaatc gtttcaaaaa atatgatgga agcctgagta
    16681 ttttatagta actcccttcc tatcaactag atcaaccagt tgagtgttga atgttgaaac
    16741 accctagcct cagcaaagga ctatgagcag catttcctag ccaataggac gctccaaatc
    16801 acctattcta aatccatcac tagcttggtc agtaagatac agactgcaag gcaagactga
    16861 cgtggttgat gagattttgg aagagtatta caatcataga ttcaccagta gacttgaccg
    16921 cagatttaac tatagtagat tgaaactaga atagtacacc tctgcttcta aaacattgtt
    16981 agaaatcgat ttgactgtcc tgatcaattt gtcctgttct cgtttcattt tactatacta
    17041 aaaaaccacc tgaaacaaat caagtgacta gaggataaaa acaaagactg ggcaaaaagt
    17101 tttgaaaagc aaaaaacgca taatatcagg tcttgaaaaa ccttgatact atacgtttga
    17161 ttgtgggaag attgacttca ttttctcctg aaattgaagt tttttctagt ttccagatat
    17221 agacgaattc aatatagcca tctcgctaca attattttaa ccatcgagaa aatttttcag
    17281 aatttcaaaa atttcatttt gatccaatga aatgtcatca ttttgaagaa tagttgactt
    17341 cacaggctct aattctatca caaagcggtg catgtctgct tttaccgcac tacttgatgt
    17401 tatagccgat aacttttcat ctcctagcaa ggaagctgtc aaacgggtaa ggtcattcaa
    17461 atgcttttta atggacttac ttaaatcttg agcacctgta gcagagcgct ctctcatatc
    17521 cagccaagct ttagccttaa aaataattaa tactcaatga aaatcaaaga gcaaactagg
    17581 aagctagctg caggctgctc aaaacactgt tttgaggttg tagatggaag ctgacgtggt
    17641 ttgaagagat tttcgaagag tatagaccac aattactcaa taccgaatac ccctgaatgg
    17701 tttctttttc atgcaccaat atattataat aatcttcatc caataataag gctgataaac
    17761 tagcatcttg gtcaaaatat aagggaattt ctcgaccgtc cttctttaat ggatattctg
    17821 gtaaaatact aaatagttca atcatagaat gaaactcagg attagttgtt gtaaatcgaa
    17881 aaagctgcgc tttctcatct ttctgacttc cttgatactc tcccaattct aaaaagtgat
    17941 tcaaggtagt ataaaattcc ttatttttta cttcatcaat gatgaccata tcataatctt
    18001 ttgttgtgcg acttttaaat ccttgcgaat ccaatacgat agaggtagca gttcccccaa
    18061 tcagaacata atagttctga aaatccgcaa acatttcttg aaaaataact ttcgtattag
    18121 ctggcatcat cttctcccag atactgtaat atcatatttt ctagtgcttc actctcttcc
    18181 tctatacgtg ggtcatcatc atcttttaag gtcaaataaa gagaaatcga atctacaaat
    18241 tgtttatcat gattattttt aaaatcattc caaaactcag atacaaaagg acgatatttc
    18301 catatctcta gcatctttca ttttaaaaca tgctgagaaa gtggcaagga taactgattg
    18361 aattttctct gccatatgac atagctagta ttttcatccg tttcagctaa aaaagttgaa
    18421 tgcgacaaag cataagcacc accatataga aggttagaaa cagattttat ctgctttata
    18481 tcgccatctg gcaataaaat ccgttttttg atgggattaa ataaacatga cacggatttt
    18541 aagaataatt ctttctttga caccgtatat gtgtaaagct tattttgctt gtttaaccaa
    18601 tataaagctt taaaagtcct caaacaccta taaattgttg agtttggaag tccagtgact
    18661 tgtgaaagca aatcaacatt tactactttt tgaccttttg tcaataaaaa ggcaatccac
    18721 gttaattgtt cgctaggtgt taattcctta gggatttcag tatcattcgc attgagtact
    18781 agtcccaatg gagggaagaa gaggtttccc ttaaagtcta caaacggaac tctagcttga
    18841 agtaattgct ttttttcact gtctgataac ttcgaaaaca ccaaaacaac atccatattg
    18901 gctttttcac ccatagtgcg agcctgagta acaaatgaac tcaaactccc ccttctcttt
    18961 tctttaatca ataaaaatga ttccttatga aaagtcattt gagtatactc aaaccttttt
    19021 acaaaactga ttggtaaagt caaagtcagt tcctcaatac tgacatcaac ataaaaggat
    19081 tgaaaaattg acattatctt tttgacattc atgttttttc cttattctat catttttatt
    19141 atattttatc attcggtata ataaaatata agttttttga taaaataacc aaaaactact
    19201 cgctatcccc tctcacatta ccctacatat cgtttgacat gcgactgata gttcaggaaa
    19261 acttcaagga gaacttttct ctcatccact atgcaggact tactatttca cttctactcc
    19321 tataggctca atttgacact tttctttttc gaattgctca tatgcctcct gaatagtgag
    19381 ttcgatttat ccaaatttat ttttgtctat tttgatgaat acttccacga agatagtctg
    19441 aaaatgaatt tgcaccaatt ttccttctct ttttaagcat attattccag atatgattgc
    19501 cctcttattc agtaataaat tacgctttaa acgcttcaat cagtatatct cctgttgtca
    19561 tatgttctaa agaaaattct tctacatatg gtttaacatc tcttaggtta ttacttccta
    19621 atatcccatt atgctttttc gctaagaaaa tagaagtcgc ttctccctta ccaataatct
    19681 tgttactatc atgatttctt gttaaatctc tatataatgc gtattcttca gttccaatgt
    19741 ctatgctcac aatctcagct gaacccttag ttaccaactg gtctatccta gattttaaat
    19801 ggggaattgt aggtatattg atttcatcat acacctcttg tggaataaca attttaccca
    19861 aatagagctt ttctaaaaga tgttcagtgc caacccataa aaatactgaa atacaatctg
    19921 catcaataaa tacacgacta gtcaaacgac aactcccccc tcttcatcta gcccatatac
    19981 aatatcatat ctgaaagcat ctagtaacag ttcctcatac ttcccttgcg aaattctgtt
    20041 attttctaaa agttgttcag ttaaattaat atattatcct gatgccattt cttttttact
    20101 ttctgacaaa ggacgatata aacttgtatc atagcctaat cttgaagctg tctctataac
    20161 actaatatcc atatttttaa tttcttctgc atcaaggtat ccatcattcc tcaatctata
    20221 taacatagct ttatgactga taccataaaa ctgacccaat tttataatat cttctacttc
    20281 aagatgagtt ctattggcat tttctctgat ttcctcaacc atcctataca gtgaagatgg
    20341 gaaaattaaa aaataagaag caaactgatc cgcttttctt tcagtttcat ctccttcacc
    20401 aatcaagata agactgactg aactcttctt cacctcatca taataaagat gatacagttc
    20461 atgtgctaaa gaaaatcttt accttcctaa tggcatgtct gaattgactg caatgagact
    20521 gaactgagtt cctttataac agactccgct aatattcttt ccgagtccat aaaataccag
    20581 cgtcaaattt tctatctttt gtaccaattt aaaaatatct atcggcgatt caccatcagc
    20641 tcccaacttt tttctaagat ttgaagcttt attacttaaa tccattcgtg atattccttt
    20701 cacctttttc cattttggca atcatgcttt gattcaaaga caaatactca gcaatcttat
    20761 cttgagtaag atttgaagat attctaagtt cttttattct ttttccaaca tcacatacat
    20821 ttatcacata catttatcat attcatacac ctctcataaa aagaatagca cactcttgtc
    20881 ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg atgctgctta
    20941 aaaatctaaa attgatgagt tcctatcaaa aatcgtatct tcaacctcaa aacagtactt
    21001 aaagctatcc gactcggttt acattgtcaa atttagattt tatttgagca taactttcta
    21061 gtttgctttc tgatttttgt ttaatatagt agcaaacatt gaaggaaata tctccacaaa
    21121 aaaacgcata ctattaagct ttttcaagac ctaataatat gcgctgttct gatttgaaag
    21181 acattccatt attattttac tgtaatcaag ccatctggct ctactgtgaa ttctggcttg
    21241 tctgccagtg ttccgtctgg tttgaggtag taccagcctg ttccgtccgc tgactggata
    21301 aaggcatttg ataccatggc gccttcttta gcgtctaagt agtaccaagt gtccttgtac
    21361 ttgacccagc ctgtcttcat ggcaccttct tcgttgaaat agtaccactt atcagcgatt
    21421 ttcttccagc ctgtagccat ttcgcctgag ttgtcgaacc agtaccagtt gccgtctgtg
    21481 tgcttcctcc agcggtctgc aagcatatag cctgaactgt caaagtagta ccaagtgcca
    21541 ttgattttct caaacttgtc ttttggataa gagccgtctg aatgtacgta ccagtagcca
    21601 gtgtcattct tctgccagcc tgtttcaatc gtcaagccgt tctcaatatc atacttaaac
    21661 tgctcacggc taatgcccca ttttgccaag taagggtatg gatccacatg gtctgagtgg
    21721 ttgtttggtt ggttattcgt gcaatactcg tgcgttttaa ttccagctaa actccctgta
    21781 tcaagcgttt tcggcaaacc tgcttcatct gctagattgc gtaagagttc gatataaagg
    21841 cggtagtccg tcatgaactc ttctttagtt gaatggcttt caatcagttc aaccgctgca
    21901 taggtctcag cattccaacc gcccccaacg tcccaggcac cattatcaac aggtcctacc
    21961 tgcatgatgc aaccgttccc aacaatgtgc gagaaaaaac ctaattctgg gtctttccgc
    22021 cagtgataat ccgcttcatt ctgtacggtt gaatgcggat tcccagttga gtgtgcgtgt
    22081 acttgcctat atggttgcac gccgacttga ggcaaatctg ttcttaattt actcacatta
    22141 atttccatat tctactcctt atcaattaaa acaactcatt ttttacaatc cagaaccaga
    22201 aactccttta tttctacctt acaaagaaga caatcttagt cacgattagg cttgtagata
    22261 gaacctcaaa acgcactatt ttgacactgt aaataggact gacaaggtct gcattctatc
    22321 tacaataaca ccccagacta aaaagctttt caaaagtata tttttacagt ctctatatgt
    22381 ccttttcata aatactatac tttattatat catataaaag aagtcaaaag tctgttaaac
    22441 tattttcaac accaaactaa agaagagaac acaagttttt tcgatgttca ctagaggaaa
    22501 tggattttat tcagtaaatc caactaggat tgcactttgg ttgccaagat tgcctttcct
    22561 tcttttatca agggatgacg gaatagtgag aagtacagtt gaactgtcat ggcaacccag
    22621 ataagaggtt cacaaaggat aacaccctta tatcctgccc aaggaataat caaaaccaca
    22681 aaaacgattt ttccgattag ttcaataaag ctagaaacta gaggaaggat cttctgcccc
    22741 aagccctgca agcaattgcg ataaatcaac aagaggctca aaatgggata aaaggctgaa
    22801 ctgatttgca gatagagact tccattttct atcaagtaac catctgtcga actagccaag
    22861 aaggaaacca aggctggact ggcaaaaaag aggaaaatac aaacaaaaac tgcccaggat
    22921 atacttaaac gactgccgat tcgaagacct tgagcaatgc ggtcaggtcg cttagctcct
    22981 agactctgag aagcaaaggt cgtcattgat gcagaaatag cggtcatagg aagaagggca
    23041 aaggtcataa tgcgtcgagc tgccgtctga gcactaataa tcactgcacc aaatgtatta
    23101 acagaagact gtaaaatcac actgccgata gatacaattg aactcatcaa gcccatagcc
    23161 aaaccctgct ccaagagatc cgcgtacaag cttttgtccc atttgaaatg tttaaactgt
    23221 ggcaagagtt ctggcacact tttacgaata taataaaagc agagaaccgc tgataaacct
    23281 tgcgaaataa tggtagcaag tcctgcggat tgaactccca gatgcaattg cgtaataaaa
    23341 tagagatcca gaaccacatt aaccaaggca gagaaaatca gaaatcccag ggctgctaga
    23401 ctgtcaccaa tagaccgcaa caagcctgca aaaagattat aagcaaagct gacacctaca
    23461 caggtcacaa tcatagaaat atattgataa gattgaggaa gaatttctgc aggagtatct
    23521 aagtattgca agagaggata caagccaaga aagcccagca acataactag aatgctcaaa
    23581 agagcaccta aaatccaggt ggctgctact gcttccttga ttttagtgaa attccgagcc
    23641 ccataataac gagcaatgac aatccccatg ccattgccaa caccaagtgt aaaacctaca
    23701 atcaggtcaa aaatcgctgt cgtcgctcct actgcagcca aggattcttg accaagaaat
    23761 cgtccaacaa tcaagacatc agcagtgtta tagagctgtt gaaaaatatt tgatagcaag
    23821 attgggaagg cgaagcttaa gagcgagaga agaatcggac catgtatcag gtctactgtt
    23881 cgttttttat tcataaagct attatatcag ctttccagag gagcgacaag aaacagcaaa
    23941 ctatatatag cggaatgaaa taaggacagt caaatcgatt tttaacaatg ttttagaagt
    24001 agatgtgtac tattctagtt tcgatctatt ataattggtt gagtttggaa tagtacgctg
    24061 tagctgctaa aacatt
//
LOCUS       DAVKHZ010000025        23986 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000025 DAVKHZ010000000
VERSION     DAVKHZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.025"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(16..3192)
                     /gene="carB"
                     /locus_tag="VWW99_000927"
     CDS             complement(16..3192)
                     /gene="carB"
                     /locus_tag="VWW99_000927"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9538791.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3504..4583)
                     /gene="carA"
                     /locus_tag="VWW99_000928"
     CDS             complement(3504..4583)
                     /gene="carA"
                     /locus_tag="VWW99_000928"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9538792.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4633..5556)
                     /locus_tag="VWW99_000929"
     CDS             complement(4633..5556)
                     /locus_tag="VWW99_000929"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9538793.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5575..6096)
                     /gene="pyrR"
                     /locus_tag="VWW99_000930"
     CDS             complement(5575..6096)
                     /gene="pyrR"
                     /locus_tag="VWW99_000930"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9538794.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6307..6936)
                     /gene="nth"
                     /locus_tag="VWW99_000931"
     CDS             complement(6307..6936)
                     /gene="nth"
                     /locus_tag="VWW99_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9538795.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6936..7478)
                     /locus_tag="VWW99_000932"
     CDS             complement(6936..7478)
                     /locus_tag="VWW99_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9538796.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7648..7965
                     /locus_tag="VWW99_000933"
     CDS             7648..7965
                     /locus_tag="VWW99_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9538797.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYDHVKHVLSLYGPV
                     FEF"
     gene            8204..9763
                     /locus_tag="VWW99_000934"
     CDS             8204..9763
                     /locus_tag="VWW99_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9538798.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRLLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9807..10706)
                     /gene="htpX"
                     /locus_tag="VWW99_000935"
     CDS             complement(9807..10706)
                     /gene="htpX"
                     /locus_tag="VWW99_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9538799.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10708..11268)
                     /locus_tag="VWW99_000936"
     CDS             complement(10708..11268)
                     /locus_tag="VWW99_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9538800.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11362..12075
                     /gene="rsmG"
                     /locus_tag="VWW99_000937"
     CDS             11362..12075
                     /gene="rsmG"
                     /locus_tag="VWW99_000937"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9538801.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12329..13612
                     /locus_tag="VWW99_000938"
     CDS             12329..13612
                     /locus_tag="VWW99_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9538802.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13807..15378)
                     /gene="ffh"
                     /locus_tag="VWW99_000939"
     CDS             complement(13807..15378)
                     /gene="ffh"
                     /locus_tag="VWW99_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9538803.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSSKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15390..15722)
                     /locus_tag="VWW99_000940"
     CDS             complement(15390..15722)
                     /locus_tag="VWW99_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9538804.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            complement(15813..16190)
                     /locus_tag="VWW99_000941"
     CDS             complement(15813..16190)
                     /locus_tag="VWW99_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9538805.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16262..17566
                     /locus_tag="VWW99_000942"
     CDS             16262..17566
                     /locus_tag="VWW99_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9538806.1"
                     /translation="MNEKVFRDPIHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17579..18385
                     /gene="yidA"
                     /locus_tag="VWW99_000943"
     CDS             17579..18385
                     /gene="yidA"
                     /locus_tag="VWW99_000943"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9538807.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            18428..19426
                     /locus_tag="VWW99_000944"
     CDS             18428..19426
                     /locus_tag="VWW99_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HEU9538808.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNDLECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(19592..19663)
                     /locus_tag="VWW99_000945"
     tRNA            complement(19592..19663)
                     /locus_tag="VWW99_000945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19628..19630),aa:Arg,seq:cct)
     gene            complement(19705..20052)
                     /gene="rplS"
                     /locus_tag="VWW99_000946"
     CDS             complement(19705..20052)
                     /gene="rplS"
                     /locus_tag="VWW99_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9538809.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(20171..20500)
                     /gene="crcB"
                     /locus_tag="VWW99_000947"
     CDS             complement(20171..20500)
                     /gene="crcB"
                     /locus_tag="VWW99_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9538810.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(20494..20868)
                     /gene="crcB"
                     /locus_tag="VWW99_000948"
     CDS             complement(20494..20868)
                     /gene="crcB"
                     /locus_tag="VWW99_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9538811.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(20865..21131)
                     /locus_tag="VWW99_000949"
     CDS             complement(20865..21131)
                     /locus_tag="VWW99_000949"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9538812.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(21247..21690)
                     /locus_tag="VWW99_000950"
     CDS             complement(21247..21690)
                     /locus_tag="VWW99_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9538813.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            21794..22729
                     /locus_tag="VWW99_000951"
     CDS             21794..22729
                     /locus_tag="VWW99_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9538814.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            22783..23067
                     /locus_tag="VWW99_000952"
     CDS             22783..23067
                     /locus_tag="VWW99_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9538815.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(23124..23342)
                     /locus_tag="VWW99_000953"
     CDS             complement(23124..23342)
                     /locus_tag="VWW99_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538816.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(23339..23913)
                     /locus_tag="VWW99_000954"
                     /pseudo
     CDS             complement(23339..23913)
                     /locus_tag="VWW99_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7167 a         5312 c         4497 g         7010 t
ORIGIN      
        1 atcttatttt tctatttaaa tcgcttctgt aacaaagcta cgactttcaa gtaccttaag
       61 catggcatta gctgtatcta gcgctgtgaa gaggggcacc ccgtgttcaa tggctgaacg
      121 acgaatttgc tcaccatctt cgtcagcagt tcgttttgtt cctactgtgt taatgatagc
      181 ttgaattctt cctttgcgta caaaacttgg gatatcctta tcgtcatcac caatcttacc
      241 aacaggttgg gcttgcaagc catgactagc aaagaaggct gctgtccctt ctgtcgcaag
      301 gattccataa ccaatgtttt ggaaacgacg agccaagttc aaggcttctt ctttggcatc
      361 atcagcgatg gtaaagacga cattaccaaa agttggcaag tgtagataag aagcttcaaa
      421 ggctttatag agagcttttt ccaaagtagc atcagaaccc ataacttcac ctgttgactt
      481 catttcagga ccgagcaagc tgtctacctt agctagtttg gtaaaggaga agacaggtgc
      541 cttgatatga acacgggtgc tttcagggta aagtccattt tggtagccaa gttctgataa
      601 actttgacca agaatgagtt tggtcgctac ttgagccata ggaatattgg ttaccttaga
      661 taggaatgga acagtacggc tggcacgtgg attgacctca ataacgtaga ctttttcatc
      721 cttgataaca aactggatgt tcatcattcc aaggcagtga agaccgattg ctaagcgttt
      781 ggtgtagtct gcgatggtct cctgaacctt ttgcgacaag gtttgtggtg ggtaaacagc
      841 cattgagtca cctgagtgga caccagcacg ttcgatatgc tccatgatac caggaatgag
      901 tacattttta ccatctgaaa tggcatcaac ttcgcactct tgcccaacga tataagagtc
      961 gacaagaact ggatggtctg gactagcctt aacagcagtt cgcatgtaag aacgaaggtc
     1021 ttcttcgttt tcaacgattt ccatggcacg tccaccaagt acataagatg ggcggacaag
     1081 aactgggaag ccaatcttac gagctgcaag agctgcttct tcttcattgg tagccgtttg
     1141 tcctggtggc tgtggaatat ccaattcttt gagagcttgc tcgaagaggt cacggtcttc
     1201 ggcacgatct aggtcagcaa cctgtgtacc aaggatggtc acacctgctt ttgccaatgg
     1261 ctccgcaagg ttgatggctg tttgaccacc gaactgaacg ataactccct ttggttgttc
     1321 caagtcaatg acgttcataa catcttcgaa tgtcaatggc tcaaagtaaa gcttatctga
     1381 tacagagaag tctgttgaaa cggtctctgg gtttgagttc atgatgatag cttcataacc
     1441 agctgcctgg atagccttaa cagagtgaac ggttgcgtag tcaaactcaa ccccttgacc
     1501 gatacggatt ggacctgaac ctaggacaag tacagattct ttatcagatc tgatagattc
     1561 attttcccaa ccataggttg aatagaaata tggcgtttcg gagtcgaact ctgccgcaca
     1621 agtgtctacc atcttataaa ctggaacaat cttgttttcc aagcgaagtt ggcgaacttt
     1681 atcatcagtc gttccccaga gttcagcaat cttacggtct gaaaaaccat taagtttggc
     1741 tgttttcaaa acttctaaat cttgtggatg agcacccaat tcttgctcaa tttcaaagat
     1801 atgcaagagt ttatcaagat agaagatatc aatttttgta agctctgcaa tttcttcagg
     1861 tgtgtagcca cgacgaatgg cttctgatac gtagaagaga cggtcatctt gggctttgac
     1921 aaccttttca atcaaggcat catcagaaac tgctgcaagt tcaggtattt cattgtggtg
     1981 caccccaatt tcaagggagc ggcaggcctt gagaagagat tcctcgatgt tacgaccgat
     2041 tgccatgact tctccagtcg ccttcatttg tgtaccgaga cggcgttcac ccttttcaaa
     2101 cttgtcaaat gggaaacgtg gaatcttagc aactacgtag tcaagggctg gttcaaacat
     2161 ggcataggtt gaacctgtaa ctgggtttat aacctcatcc aaggtcaaac ctactgcaat
     2221 cttggcagcc aacttagcaa tcggatatcc tgtcgcttta gaagcaaggg ctgacgaacg
     2281 tgatacacga gggtttactt cgataacata atacttgaag ctgttaggat caagagctag
     2341 ctgaacatta catccacctt caatcttgag ggcacgaata atgctcaagc tcgcatcacg
     2401 aagcatttgg ttttcatagt ctgacatggt ttgcgcaggg gcaaatacaa tggaatcccc
     2461 tgtgtgaatc ccaactgggt caaagttttc catgttacaa acaaccaagg cattgtcagc
     2521 tgagtcacgc atcacttcgt attcaatttc cttgaaaccg gcaatcgaac gctcaatcaa
     2581 acattgggta acaggtgaca atttcaaacc attttcagtg atttcacgca attctttctc
     2641 gttggcacac ataccaccac cagtaccacc aagggtaaag gctggacgaa cgatgactgg
     2701 gtagccaatt gtcgctgcaa aggcaactgc ttcttctact gtgttaacaa tttcagattc
     2761 tggaatgggt tgttcaagct cttccatcaa ttgtttaaag aggtcacggt cctccgcttg
     2821 gtcaatggca gataatttgg tacccagaag ttcaacgcca agctcgtcta ggataccatt
     2881 tttagataat tccatggcca tgttgagacc tgtctgacca ccgagtgttg gtagcaaggc
     2941 atctggacct tccttacgaa gaatacgtgt cacaaactca agtgtaatcg gttcaatgta
     3001 aaccttgtca gcaatttcct tgtccgtcat gatggttgca ggatttgagt taaccaaaac
     3061 aacctcataa ccttcctctt tcaacgacaa gcaagcctga gtcccagcgt agtcaaactc
     3121 agcagcctga ccaataataa tcggaccaga accaatcacc ataatttttt gaatatcagt
     3181 acgtttaggc atagtttatg atacaaagcc cgtaaagaac acagtgaaaa taggaaactc
     3241 ggcgcagacg ccttcagcgt caagacgatg tttatctttt tcacacagtt cttagggcgt
     3301 gttcacttcc gcgaacaatg tatcttctcc tttctattcg tcgcctctca gggcgacatt
     3361 aaataagata caaaggacga atagaaagcg attgaatttt aggaaatcaa ggaaggattg
     3421 acaatccaag ttggtttctc tacattctga gctttccgtc cgtgttcagt tacataaatt
     3481 ctccgacgag cttttactcg ttcttagttt gattgtttaa aaacttccat catctcgata
     3541 aactcgtcaa ataggtagct agcgtcgtgt ggcccaggag ctgcatctgg gtggtattga
     3601 acagagaaag caggttggta tctgtggcgc acaccttcca ctgacttgtc attgatttct
     3661 tcgtgggtaa taatcaagtg ctctggcaaa tcctcgcggc tgactgcata accatggttc
     3721 tggctggtga agtctactcg tcctgttgcg atttcacgta ccgcatggtt gaatccacgg
     3781 tggccaaact tcatcttata ggtcttagcc ccgtttgcca ttgcaaagag ttggtgtccc
     3841 atacaaatac caaagattgg aatttttcct tgtacaccgc gaatcatgtc gagtgcttgt
     3901 ggaacgtctt ctgggttacc tggaccattt gacaacataa ctccgtcagg attgagatgg
     3961 agaatttctt cagccgttgt cgaataagga acaactgtca cgttacagtt gcgtttagaa
     4021 agttcacgta ggattgagtg cttgagacca aagtccacta gcaccacgct caaaccaact
     4081 cctggagctg gataagacgt tttagtagaa acctgtttga tattgtctgt cggtaaaact
     4141 gttgcttgga gctggtccgt cacatggtcc atactgtccc caacatgggt caaggttgca
     4201 cgcatagtac catgcttacg gataatcttg gtaagagcac gcgtatcaat tcctgaaatc
     4261 cctggaattt tcttggcttt caaaaattca tccaaggtca tttggttgcg ccagttgcta
     4321 gctctacgcg cttcttcaaa aacaacgact cccttacaag ttggaataat ggattcataa
     4381 tcatcacgat taataccata atttcctacc aaaggataag taaaggtcaa gatttgtcca
     4441 ttataagact ggtctgtaat ggattcttgg tagccggtca tccctgtatt aaagacgatt
     4501 tcgcctgtta catcaatatc tgctccgaag gccttgcctt caaaaactgt gccatcttct
     4561 aatactagaa ttctttttgt catattttca cctctcgtgg acgctcactg gcgtctttta
     4621 acgtcttgtg ttttagttgg cgtttctact cgctagtacg gattctaaga ttgccattcg
     4681 aacaaagaca ccattggtca tttgttggac aatccgtgat tttggtgctt caaccaagtg
     4741 gtctgctatt tctacatcac gattgattgg agctgggtgc atgaggattg ctgtttcttt
     4801 caaacgatcg taacgttctt gagtcaagcc atgttgggca tggtagtctt cttttgaaaa
     4861 tacagctcca ctatcatggc gttcgtgttg cacacggaga aacatcatga catcaacctg
     4921 atcaatgatt tcatcaatgg ttacaaactg tccatagtct gcaaactctt gacttctcca
     4981 ttcctcaggt ccagcgaaaa agagttcagc tcccaagcgt ttcaaaatct gcatattgga
     5041 tttggcaacg cgtgagtggt ccaagtcacc tgcaatagca actttaagac cctcaaagtg
     5101 gccaaattcc tcataaatgg tcatcaaatc aagcaagctc tggctagggt gttggcccga
     5161 accatctcca ccattgatga tggaagtcgt aatcgttgga ctagcaatca attctctata
     5221 gtagtcgacc tctggatggc gaatcacaca gacatccact cctaaagcag acagagtcaa
     5281 aatggtgtca taaagtgtct cacccttatt aaccgagcta gtcttcacat caaagtcaag
     5341 tcgttccaat ccaagtttaa tctctgcgac ttcaaaggac ttatgtgtcc gtgtagaatc
     5401 ctcaaagaag agattggaaa caatcggatg gtcttcatag ggaagctggg ctccattttt
     5461 aaactcaatt cctcgcttga tcaatttcat tacttgatcg acagtgaggt cttccatgga
     5521 caccacatgg ttcaatgctt gttgattttc tgacatggct actcctttaa ctttctaagc
     5581 ttcttcagta atcagaactc tgtcttggtc atcaagttct gtcatctcta cgatgatttc
     5641 ttcagaacga ctggttggga tattttttcc aacgtaatct ggacggattg gcaattctct
     5701 atgtccacga tcgactagaa ctgctaaact cacacgcgca ggacgaccat gaccgacaat
     5761 attatcaata gcagcacgga tggtacgacc tgtatagagc acatcatcca ccaagataac
     5821 ttcgcggtct gtcacatcga cagaaaccaa agaagtatct tctccacttt taacatcatc
     5881 acggaaaggt ttagtatcca attccacaac aggaactgaa agattttcta actgcttcaa
     5941 acgttcttgg attcggtggg caataaagac accacgagtt ttaataccag ccaagacgat
     6001 cttattcaaa tctttgttgc gttcgataat ctcataagta atacgcgtaa tcgctcgttt
     6061 gacggtcaat tcgtctacaa cttcttttgt tttcatgaca aacctccaaa aagaaaagtc
     6121 tccttaaaca aggagacttg aaatttatag ccaagcgagc cctactgcac acagtataga
     6181 cttcaccctt ctactttatc gcgctccttg cctgcctcac gggacaggtt taaaggaata
     6241 tttagttatc atttactata gcacaaagca tgcttaaaat caagcaaaaa gtttcaatgt
     6301 agcatcttac aaattgctaa aatcatataa ttgtgggtac tggtcacact ctggattttt
     6361 tggatggcaa atggctcttc caaaataaat catggcctga tgggcagcta accactgctc
     6421 aggcggcaag atatccatga cccgcttttc cacctcaagt ggcgtcgctg attttttgac
     6481 aatatcgtgg tgtttgcaaa tacgctccac atgagtatcc actgcaaagg ctggaattcc
     6541 aaatcctaca ctcatgacaa cattggctgt cttgcgacca acacctgcca aactctccaa
     6601 ttcttcacgt gtctgaggga cttgaccatc aaaatcgtct agtaactgtt gggcacattt
     6661 tttaaggaat ttagctttat tccgatacaa tcccaagcga gaaatatgtg aagcaatctc
     6721 actctctgtc gctacagaca tagcttgggg tgttggaaag gcaacaaaga gacctggtgt
     6781 ggccttattt accgctgcat ctgtcgtctg ggctgataac atgaccgcaa ccaggagttc
     6841 aaaatgattg gtaaaatcaa gactaggctt ggcatctggg aagagggcaa tgatttcttc
     6901 tagcaccttt cgtgctcgtt tttttgacaa gaccattatt catctccgtc aaatagtcct
     6961 tgtaagccag caaaaggact gttttcttct ttctttactg ctttttgagc ttggtattct
     7021 tcctctgtca tgatttgcca gtcatttcct gagataaatc cttgaccagc ttcttcttca
     7081 gccgtcaaga ccttgatagg aatgtttagc aggatattgt ctgatacact ctcagcaagg
     7141 tcaagctccc cattttcgat gggcaagacc aagtcatcat ctaaaacttc ttgatctagc
     7201 tggttagttg cgccttccat gaaaacttcc gtgactggat aagattcaac taactcaact
     7261 ggctccatac tgcgactcga agcaagaaca atggtataag atagttgata atctaagaaa
     7321 tacatacggt cttcatattg tactttccca actgcaagga tatcttttac atctaaaatt
     7381 tcttgattac gtgcacgcag gtcatcaact aaatctaacg tttgttcaaa gttcaaacct
     7441 tcagactgct tacgaatttc ttgaatattt aatttcatac ttcctccata aagatttact
     7501 ctcttgatta taccatgaaa aggctacaaa tcagcacacc aaactttgta attaaaattc
     7561 aaaattttaa catatttact atgatagttt tattttttag tgctatacta tagggaaaga
     7621 gtacatcaga tcaaggagga tgctcacatg gaagacaaga aactcattca actcctatcc
     7681 aagttaaata aaagctacca aaactgtaaa cagggtacgg cagatgatat tcgactacaa
     7741 gagctgctaa acactactat gcaagagctc aaaaaaacgg aacagttgaa caacagtatc
     7801 ttaattgatc ttgagaaatt ttaccaacct accagtcttc tgattggact gggtagccta
     7861 aaactaaacg atcaagcacg cactgcttgg cgaaactatg ataaattcca ttacgatcat
     7921 gtcaaacacg tactaagtct ctatggacct gtttttgaat tttagagcat agaatttcca
     7981 gttttctgtt gacaaaattt ccttaaaggt ataatataaa gatactaata ctcggaggta
     8041 agggagacat gaacaactaa gtctatcaaa taaagaacct ttatttagta gatcttgttt
     8101 ttgtctcttt ttgtgtgctc ttttatgctc tttttctggc atgttaatag agtttttttg
     8161 acatagactt tgggctctac taggtaaagt agagcttttt gttatgcact atgaacattc
     8221 tagaaaggga aatcatatga taaaaatcaa tcatctaacc atcacacaaa acaaagattt
     8281 acgagatctt gtatctgacc taaccatgac catccaagac ggggaaaagg ttgctattat
     8341 tggtgaagaa ggaaatggca aatcaacctt acttaaaatt ttaatggggg aagctttgtc
     8401 tgatttcact atcaagggaa acatccaatc tgactatcag tcactggcct acattcctca
     8461 aaaagtccct gaggacctaa aaaagaaaac tttacacgac tacttctttt tagattctat
     8521 tgatttagac tacagtatcc tctatcgttt ggcggaggaa ttgcattttg atagcaatcg
     8581 tttcgcaagt gaccaagaga ttggcaatct atcagggggc gaagctttga aaattcagct
     8641 tatccatgag ttagccaaac cctttgagat tctattttta gatgaacctt caaatgacct
     8701 agaccttgag acagttgatt ggctaaaagg ccagattcaa aagaccaggc aaaccgttat
     8761 tttcatttcc catgatgaag actttctttc tgaaacggca gacactattg ttcacttgcg
     8821 actggtcaaa caccgtaaag aagcggaaac gctagtagag catttagact atgatagcta
     8881 tagtgagcag agaaaggcta attttgccaa acaaagtcag caagctgcta acaaccaaag
     8941 agcctacgat aaaaccatgg aaaaacatcg gagagttaag caaaatgtag aaactgcgct
     9001 tcgagctacc aaagatagta ctgccggtcg cctattggct aaaaagatga aaactgtcct
     9061 ctcacaagaa aaacgctacg aaaaggcagc tcagtccatg actcaaaagc cacttgaaga
     9121 ggaacaaatc caacttttct tttcagacat ccaaccatta ccagcttcta aagtcttagt
     9181 ccaactggaa aaagaaaatt tgtccattga cgaccgagtt ttggttcaaa aactacaact
     9241 aactgtccgt ggccaagaaa aaatcggtat tatcgggcca aatggtgttg ggaaatcaac
     9301 tctgttagcc aagttacaga gacttctgaa tgataaaaga gagatttcac ttggttttat
     9361 gccacaagat taccacaaaa aactgcaatt ggatttatcc ccaatagcct atctcagtaa
     9421 aactggggaa aaagaggaac tacagaaaat ccaatctcac ctagctagtc tcaatttcag
     9481 ttatccagaa atgcagcatc aaattcgctc cttatctggc ggacaacagg gaaaactcct
     9541 gcttttggat ttagtcctgc gcaaaccaaa ctttctcctg ctggatgaac ccacacgaaa
     9601 cttttctccc acttctcaac cccaaatcag aaaactcttt gctacctatc caggcggtct
     9661 catcactgtt tcgcatgacc gtcgtttatt aaaagaagtc tgctcgatca tctatcgcat
     9721 gacagaacac ggtttgaagc tagttaattt agaagattta taaatttgca acatagcaaa
     9781 aatccagaga cgacctctgg attcttttac atctgtttta aacgttcaat ccgttctgag
     9841 ataggtgggt gggtataaaa gagtttttgg aaccccccac ctttcttagg atcattgata
     9901 taaagggcac tgctagcatc atcgacgtgg cgactcatag gtttgctatt gtccaactta
     9961 tctagggcat taatcattcc ctggggattg cgagtcagct cgacactaga tgcatctgcc
    10021 agaaattccc tctgacgaga aatagcgagc tgaaccaagg ttgcagcgag aggtgccagt
    10081 acaatagcta gtagggaaac cactagcata atgatttcaa gaccatttcc atctcggtca
    10141 tcatcacttc gtctgcgacc tgctccaccc caccacatca tacgacctgc catactagaa
    10201 agcatggtga tagcactagc aagggcaact gcaatagtcg aaatacggat atcataatta
    10261 cgaatatgac tgacttcatg tcccataaca gcttctagtt cttcacgatt catgatagct
    10321 agtagacctg aagtcgcagc aacagccgca ttttgaggat tagaacctgt cgcaaaggca
    10381 tttaaggctg gatcatcaat gatgaaaaca cggggcatag gaatctgagc gaccagagcc
    10441 atatcttcca ctacatggta gaggtctggt gccgtttgct catccacctc acgcgctcca
    10501 ttcatggaca tgacaatctc tgtcgattga aaaatcatag acaaagcgta gataaagccg
    10561 ataatcagtg caataaccaa accaccaagt ccagatctta taaagagata accaaccgca
    10621 taaccaacaa gagctaagag taggaaaaat accagcaaca aaatccaggt ttttcgttta
    10681 ttgcttgcaa tttgatcaaa caacatctta gtcacctaaa ccgctaaaat caactttagg
    10741 aaccgacttt tcctcttcag gtgtttgaag gaaatctgcc gctttaaatc caaacattcc
    10801 agcgataata ttgctcggga aagtttctaa ttttacattg tagttgctga caacactgtt
    10861 atagagttga cgagagtaag aaattttatt ttctgtgttt gtcaactcct cttgcaattt
    10921 aacaaagtta gcactagctt tcaaatctgg atagctttct gcaactgcaa aaatacctga
    10981 aacctgacga gtgagggcat cactggcttt catagcttct gctggtgaag tcgctgccgc
    11041 cacttggtta cgtagttctg ccaccttttc aagggtagaa ccttcatatt tggcataacc
    11101 ttttacagtc tcaatcaagt ttggcaagag gtcattgcga cgtttcaact gaacatcaat
    11161 ctgactccaa gcctccttgg tttgcatacg atttttaacc aaaccgttat agctaacaat
    11221 cacaaaaata acaataagag cgataactcc aagaataatc caagtcataa tataagtcct
    11281 ttctgctttt agattagtac cagtatatca aattttctat gattgtggta aaataagatg
    11341 atactaaaga aggaaataac tatgaaacca aaaacatttt acaacttgct tgccgagcag
    11401 aatcttccac tttcggacca gcaaaaagaa caatttgaac gttattttga gctcttggtc
    11461 gagtggaatg agaagattaa tttgacggcg attacggaca aggaagaagt ttatctcaaa
    11521 catttttacg attcgattgc acccattctt caaggtttga ttcccaatga aactatcaaa
    11581 cttcttgata tcggggctgg ggcaggattt cctagtctac caatgaaaat tctctatccg
    11641 gagttagatg tgaccattat tgattcactc aataagcgca tcaacttcct acaactcttg
    11701 gctcaagaac tggatttgaa cggagttcat ttctaccacg gacgtgccga agattttgcc
    11761 caagacaaga acttccgtgc tcaatatgat tttgtaacag ctcgtgcggt tgcccgtatg
    11821 caggtcctat ctgaattgac tattccctac cttaaggttg gtggcaaact attagcactc
    11881 aaggctagca atgcgcctga ggaattatta gaagctaaga atgccctcaa tctccttttt
    11941 agtaaggtcg aagacaatct cagctacgcc ctaccgaata gagatccgcg ctatatcaca
    12001 gtggtagaaa agaaaaaaga aacaccaaat aaatatccac gtaaggctgg tatgccaaat
    12061 aaacgcccac tttaaatttt ttagtaaaca aatgtttaca aaatcagcct cgctctttta
    12121 tttctaggct cgggaaaaaa tgatttacaa aatcattttt ttctgctata ctatcctaag
    12181 caaaggtttt taatgtcatc ccgtgaggtg acgaagacgc agaaatattt aaaactcttt
    12241 aaaatctaaa ttttaaagaa gtcttactct gagggcctat tgctgtaaaa taatgggctc
    12301 ttttttgatg cccaaaagtg aggtttatat gaaacaagaa tcaactgttg atttgttact
    12361 agacgttgac caacgtcctt cggctggaaa aggaattctc cttagtttcc aacacgtttt
    12421 cgccatgttt ggtgcgacca tcttggtacc attgattttg ggaatgcctg tatctgttgc
    12481 cctttttgct tcaggtgttg gaacactcat ctacatgatt gctactggtt ttaaagttcc
    12541 agtttatcta ggttcttcat tcgcctttat cacagctatg tcactggcta tgaaagaaat
    12601 ggggggggat gtatctgctg ctcaaacagg ggttatcttg actggtttgg tctatgtcct
    12661 tgttgctacc agcatccgat ttgtaggaac aaaatggatt gataaactct tgccaccaat
    12721 cattatcggt cctatgatca tcgttatcgg tcttggactt gcaggttcag ctgttaccaa
    12781 tgcaggtctt gtagcagacg gaaattggaa aaatgctctg gtagccgttg ttactttcct
    12841 aattgctgcc tttatcaata caaaaggaaa aggcttccta cgaatcattc cattcctctt
    12901 tgccattatc ggtggttacc tcttcgcgct aactcttggt ttggttgact ttacgcctgt
    12961 cctagaagct aactggtttg aaattcctgg tttctacttg ccatttagca caggtggtgc
    13021 ctttaaagag tacaatcttt actttggtcc agaagccatc gctatcttgc caatcgctat
    13081 cgtaacaatt tctgaacata tcggagacca tactgttttg ggtcaaatct gtggccgtca
    13141 attcttaaaa gaaccaggtc ttcaccgtac tcttattggt gacggtatcg caacttctgt
    13201 ttctgccttc cttggtggac cagccaatac aacttacgga gaaaatacag gggttatcgg
    13261 tatgactcgt atcgcttctg tctcagttat ccgtaacgct gccttcatcg cgattgccct
    13321 cagcttcctt ggtaaattca ctgccttgat ttcaactatt ccaaacgctg tacttggtgg
    13381 tatgtcaatc cttctctatg gggttatcgc cagcaatggt ttgaaagtct tgattaaaga
    13441 acgtgttgat ttcgctcaaa tgcgaaacct catcatcgca agtgctatgt tggttcttgg
    13501 acttggagga gctatcctta aacttggtcc agttacactt tcaggtactg ccctttcagc
    13561 catgacagga atcatcttga acttgatctt gccatacgaa aataaagact aagagtctaa
    13621 atacacctaa tccactcaga cagctgagtg gatttttcgt atactataat aaaagtgtct
    13681 taacaaaatt attaaaatca aaaaacgtat aatatcagat attctaaaac cttgatactg
    13741 tacgttttat catagaaatt tttactttat tttctcatca aatgagattt gcatcaatct
    13801 cttgtcttac ttgcgtttct tcttcgcttt cttcattttg ttagccatac gtttcatgga
    13861 ctgtttcatg gcaaattcac caattttacc tttcaaaccg ccaccaaaca tctggctcat
    13921 atctggcatt cctgctcctc cgagagctga taagtcaggc ataccgcctt gtcccatcat
    13981 tccttcaagg gcagacatat ccattcctcc catatttggc atatttttag gaaggttatt
    14041 tggattaatc cccatttgct tcatcatttt attcatatcc ccagacataa caccctgcat
    14101 gagctgttta gcctggttaa agtccttgat gaatttattg acttcgacga atgtatttcc
    14161 agaaccagca gcaatacgac ggcgacggct tggatttaac aaatctggat tttcacgttc
    14221 ttcaggtgtc atcgaagaca caatcgcacg tttacgagca atctggcgtt catccacctt
    14281 catgttttga agtgctggat tgttggccat acctggaatc atcttgagca agtcttccat
    14341 cggccccata ttttgcacct gatctaattg atcgatgaaa tcattaaaat caaaggtgtt
    14401 ttcgcgcatc ttctcagcca tttcaagggc tttttgttca tcgtattcct gagaagcttt
    14461 ctcaatcaaa gtgagcatat cccccatgcc aaggatacgg ctagacatgc ggtctgggtg
    14521 gaaggtttca atgtccgtaa tcttttcacc tgtaccagtg aacttgattg gttttccagt
    14581 aatgtgacga acagacagag cagcaccacc acgagtatcg ccatcaatct tggtaaggat
    14641 gaccccagtc acttccaact gagcattaaa ctcacgcgca acattggctg cttcctgacc
    14701 aatcatagca tcaacgacaa gcaagatttc atttggttga gccaatgctt tcacatcacg
    14761 aagctcattc atgaggagct catcaatctg caaacgaccc gcagtatcaa tcaagacata
    14821 gtcgttatga ttagtttgag cttgctccaa accttgacgt acaatctcaa cagctggaac
    14881 ctctgtccca agagcgaaaa ccggcacatc aatctgttgt cccagtgtct tcagctgatc
    14941 aatggcagca ggacggtaaa tatccgccgc aatcatcaaa ggacgggcat tttcttcctt
    15001 cttgagtttg ttagccagct tacctgcaaa agttgtttta ccagctccct gcaaaccaac
    15061 catcatgatg attgtaggaa tcttagatga cttgataatt tctgccgtat cagaacctaa
    15121 aacagctgtc agttcctcat caacgatttt aataatctgt tgcgcaggat taagtgtatc
    15181 aatgacctca tgcccgactg cacgctcacg aactttcttg ataaagtcct ttacaacagg
    15241 caaggcaacg tcggcctcga gcaaggccaa gcgaatttct ttggttgcct cttggacatc
    15301 agattcagag atttttcctt ttttacgtag acttttaaag acgttctgca aacgttctgt
    15361 taaactttca aatgccattt ttcttcctct tattctctat tatcaatgct tgttaaaatt
    15421 tctatctgct cctgcagaaa gttatccttg ggatagcgct ccaaaatctg atcaaaaatc
    15481 tgactgcgga caatatagtc cgagtacatg tgcaatttca tctcataatc ttccagaatc
    15541 ttttctgttc gcttgatatt gtcatagaca gcctgacgac tgacaccgaa ctcctcggca
    15601 atttcagcaa ggctgtaatc atcagcgtag tagagctcga tataattcat ttgcttatct
    15661 gtcaaaagcg ccgcataaaa ttcaaagagc gcattcatac gattggtttt ttcgatttcc
    15721 ataactttta ttataccaaa aattagccta atctaccaca ctaggaagcc gatccaagaa
    15781 gatagatagc taaatttgaa aaagacataa gcctagcccc aagtaatttc caattgatag
    15841 ctggcaaagg gatgtccctc ttgattttgt agttgataat ctagttcaat cttttgccca
    15901 tccacttgat aacgattcgt ttggataata aactcctgca tacccatagg tgtgggaata
    15961 taggctaaac tatcactatc ctttagaaag cgcataatgg tcttgggatt ggaaaatcga
    16021 ctcatcacaa gttcttgacc atgaaattta atcactactt tttccttttc ctcattatag
    16081 aaaagcaggt agctataatc tcctttttca tgcacttcca catcataaag ctggtcaatt
    16141 acttccaact gctcatcaaa ctgaattgta tttcgcatcc gaatcttcac atcaggccct
    16201 ctttcttgtc tcttgtccta ctattttacc aaaaagagca ggattttgct ataatggtca
    16261 tatgaacgaa aaagtattcc gtgaccctat tcacaactac atccatgtca ataatcaaat
    16321 catctatgac ttgattaata caaaagaatt tcagcgtttg cgccggatca aacaactggg
    16381 aacttccagt tataccttcc acggtggaga acacagtcgc ttctctcact gtctaggagt
    16441 ctatgaaatt gcacgacgca tcacagagat tttcgaagaa aaatatcctg aggaatggaa
    16501 tcctgccgag tctctcttga ccatgaccgc tgctctccta cacgaccttg ggcatggtgc
    16561 ctactcccat acttttgaaa atctctttga tacagaccat gaagccatta ctcaggagat
    16621 tattcaaaat cctgagacag agattcacca agtcctgcta caagtggcac ctgatttccc
    16681 agaaaaggtg gccagtgtca ttgaccatac ctatcctaat aagcaggtcg tgcagctcat
    16741 ttctagtcag attgacgcag atcgcatgga ctatctcttg cgcgactcct attttacagg
    16801 agcatcctat ggggaatttg acctgactcg aatcctccga gtcattcgtc ctatcgaaaa
    16861 tggtatcgcc tttcagcgca atggcatgca cgccatcgaa gactacgtcc tcagtcgcta
    16921 ccagatgtac atgcaggttt atttccaccc cgcaacacgc gccatggaag ttctcctaca
    16981 gaatcttctc aaacgcgcca aggaactcta tcctgaggac aaggatttct ttgcccgaac
    17041 ttctccacac ctcctgcctt tcttcgaaaa aaatgtgacc ttgactgact atctggctct
    17101 ggatgatggc gtgatgaata cctacttcca gctttggatg accagtcctg acaagattct
    17161 tgcagattta tcgcatcgct ttgtcaaccg caaggtcttt aaatccatta ccttttcaca
    17221 agaagaccaa gaccaactcg ctagcatgag aaaattggtt gaggacatcg gctttgatcc
    17281 cgactactat actgccattc ataagaactt tgacctccct tatgatattt accgtcccga
    17341 atctgaaaat ccaaggacac agattgagat tttacaaaaa aatggagaac tggcagaact
    17401 ctctagcctg tcccctatcg tccaatccct tgctggaagt cgccacggag ataatcgctt
    17461 ttatttcccg aaagaaatgt tagaccaaaa cagcatcttc gcaagcatca cccagcaatt
    17521 tttacacttg attgagaacg atcattttac cccaaataaa aactaaaaga ggaaatttat
    17581 gagtattaaa ctaatcgccg tcgatattga cggaacccta gtcaacagcc aaaaggaaat
    17641 cactcctgaa gtcttttctg ccatccaaga tgccaaagaa gctggtgtca aagtcgtgat
    17701 tgcaactggc cgccccatcg caggtgttgc caaacttctg gacgacttgc agttgagaga
    17761 ccaaggtgac tatgtggtga ccttcaatgg tgcccttgtc caagaaactg ctactggcca
    17821 tgagattatc agcgaatcct tgacctatga ggattatctg gatatggaat tcctcagtcg
    17881 caagctcggt gtccacatgc atgccattac caaggacggt atctatactg caaatcgcaa
    17941 tatcggaaaa tacactgtac acgaatcaac cctcgtcagc atgcctatct tctaccgtac
    18001 ccctgaagaa atggctggca aggaaattgt caagtgtatg ttcatcgatg aaccagaaat
    18061 tctcgatgct gcgattgaaa aaataccagc cgaattttac gagcactact ctatcaacaa
    18121 atctgctcct ttctacctcg aactccttaa aaagaatgta gacaagggtt cagctattac
    18181 tcacttggct gaaaaactcg gattgaccaa agatgaaacc atggcaatcg gtgacgaaga
    18241 aaatgaccgt gccatgctgg aagtcgttgg aaaccccgtt gtcatggaaa atggaaatcc
    18301 agaaatcaaa aaaatcgcca aatacatcac taaaacaaat gacgaatccg gcgttgccca
    18361 cgccatccga acatgggtac tgtaaaagta tcatttttca ataagaattg attagcaata
    18421 aaatccaatg aattttttta gcaaactatt taatttaaaa caaaataatc ataatagaga
    18481 cacaaattct gattgtaaca atttttacct aaacgattta gaatgtggcc ttactcctgg
    18541 gcaactcata ctcatagatt ggactcaaaa aacagggaga aattataatt tcccaagata
    18601 ttttaaatac tctcttcaaa ttgaccctga atctacacac aatcaattat acaaattagg
    18661 atacttcact aaaaataaga ctttatcata tcttacagta gtagaattaa aaactatatt
    18721 atctaaacat aatttagcta cttctggaaa aaaagcagaa ttaattacaa gaataattaa
    18781 taatgttaac attgacaatt tagatattcc gttcgaattt aaactaacaa aagaagcaca
    18841 aaatcttatt atcgaacata gtgactatat caaagcatac tatgataaag acataactat
    18901 ggaagattat tgtaaagaaa aaaacaatat ctcttttaaa gcaacttttg gtgatataaa
    18961 atggagtctc ttaaataaac aagctcatag gaatactgta tcaggagatt ttggatgctt
    19021 atctaacaca cgaaaggctc agggaagaca tttggaacaa gaaggtaata ttaaacatgc
    19081 tttaatatat tacatagaat ctttgataat tactatttca ggattagaaa acaatttttc
    19141 agccactgat tatccagtat attatcccga ttcgatacct gactactcac taaaacatat
    19201 tcaaacatta atggaatcat tatctgatga cgattatgat tttgcttttg atgaagcatt
    19261 atttcgcttc tcaattttga atgcaaatca ttttttatct aaggaagata ttgactattt
    19321 aagagttaat ttacctcgtt ccactgctga agaaataaac aattacttaa agaaatatga
    19381 atgttatagt cctttaaata atttagaact tgacgatttt gaataaattg actatacaaa
    19441 catttatata ctcgatatag tctcaatttt atctgatgat tgcccaaatt tttcaataat
    19501 aaaacgcata atattatgga gacaatcccc tatattatgc gttcttttaa tatcaaagac
    19561 tttttgacaa acttcttcga tatctaatta ctgccccctg caggaatcga acctgcaact
    19621 actccttagg agggagttgt tatatccatt gaactaaggg agctagataa aaactctgct
    19681 gaaaagcaga gttttttagt cgaattaacg acggatttct ttgatacgag ctgccttacc
    19741 ttgaagagca cgcaagtagt acaatttcgc acgacgtact ttaccgtaac gaacaacttc
    19801 gattttttca acacgtggag tgtggattgg gaagatacgc tcaacaccta caccgttaga
    19861 gattttacga actgtgtagt tttctgagat tccagcacct ttacgtgcga taacaacacc
    19921 ttcaaaaatc tggatacgtt cacggttacc ttcgacaact ttcgcatgta cacgaacagt
    19981 gtcaccagga cggaatgatg ggatatctgt acgaagttga ccttcagtca agctttggat
    20041 taatggattc attttattct cctatctttg tcaatcttga ggaatcttcc tcagcggata
    20101 aactgtattt ttgtgcgtcc attacacaca agatatagtt taccaaattt ctaaaaaaaa
    20161 gtaaagaaat ttatagcaaa attcctaaaa aaatcgcaac caaaccacct atgtaagtca
    20221 aagtcaggta agaataaaag accttcttat cacttagcaa tctttgaagt tcgtcattca
    20281 aggtcgaaaa agttgttaaa ccgccacaaa atcctgttgc tagaatagca tatacttcct
    20341 tagactctac atgattgtag aatactccaa ttaaaaaaca acctaaaaga ttagctatga
    20401 gcgttccgag tggcaattta gaagcttgat tatagcgaga aaagaaatac cgtacgagtg
    20461 ctcccaagcc acaggcgatt gcaagataga cgattaccat ttcttcctcc ccaaatagta
    20521 agctaaaagc aggcctccac cgatactcaa aagcaaatac agtatcaaac taagataacg
    20581 ccctgtatca agcagcttca cagtatcaag cattaggctg gaaaaagttg ttaaacctcc
    20641 gcaaaaaccc gtccccagtg ctaaaatcaa gcccttacta gtccccttat agaccagata
    20701 gcccttgaca agataaatca agcagaaaat tcccagatag ttgacaaaga gagtgcccca
    20761 agggaaatct ggactggctg gtaaccaagt ggaaactagg tagcggacaa gtccgcccaa
    20821 catagcagct agaaaaatcc ctagcggata aaattgttct tttttcattt gatattttga
    20881 tcctgataat cacgcgaacg tttgagtatg tccgaaaaag tcgcgacaat agtctcctga
    20941 tagcgcttgt cctctacacg acttctaacc ttttcaaaaa taactgcttc tctcttagta
    21001 tctaaaatcg gtttccctga agccttctta taagcgacaa ctccttcaac taagtgcatt
    21061 cgttcttcta acagtttgac aatttggtcg tcgatttgat cgatttcttg acgaataata
    21121 tctaaatcca tacagtctcc tcctttattt gaattattgt atcaaaaagc catcaattag
    21181 gctagtaaaa tcccaactct cgataagaaa aatgcactga ttgattacat cagcgcactt
    21241 ttatgcttat cctactttag cagccaattc ttctgcgaat tgttccaagc gttcaatatc
    21301 ttcttcctcg gcagaaagat caactttaac acactctgaa cctttttctg ctcctgttga
    21361 cacaaagaca cggtcaaagt catcaacagc cttacaaaat tcatcgtaga aggtatctcc
    21421 tgagccgacc actccgtaga ttttgccatt caagttgaga tctgctaggt cttcgtagaa
    21481 gtccatcatc tcatctggca attcaccatc accataagta taggtcgcaa cgatagcgat
    21541 gtctgcttcc aagaagtctg aagcgtcaac agttgtacat tcatcaacat caacatccaa
    21601 gcccaagtca cgtaatttgt ctgctacaat atctgcaatt tcttcggtat taccggtcat
    21661 actggcaaat acaatttttg ctaatgccat atcgtcctcc tcaatttatc tttctccatt
    21721 atatcacatc tttttcattt taaaaataaa aattcttgtg ctacaatgaa atgagaaaga
    21781 attgaggtta tttatggaga tttgccaaca aattttagaa aaaattaaag aatacgacac
    21841 cattatcatt catcgtcata tgaaaccaga ccctgatgcc ttgggaagtc aggtgggatt
    21901 gaaagccttg ctagaacatc atttcccaga aaaaaccatc aaagccgtcg gttttgatga
    21961 accaactctt acttggatgg ctgagatgga tcttgttgaa gatagagcct accaaggcgc
    22021 acttgtcatc gtctgtgata cagctaatac tgctcgtatc gatgataagc gctatagtca
    22081 aggtgatttt ctcattaaga ttgaccacca tccaaatgat gatgtatacg gtgacctgtc
    22141 ttgggtcgat actagttcaa gtagcgctag cgagatgatt accctatttg cccaaacaac
    22201 ccaactagcc ttggcagatc gcgatgctga gttgctcttt gcaggaattg tcggtgatac
    22261 aggtcgcttc ctctaccctt ctaccactgc acggactctt cgcctggctg cttatttgag
    22321 agaacataac tttgactttg cggctctcac tcgcaaaatg gacactatga gctacaaaat
    22381 tgctaaactg caaggctaca tctacgacca tctggaagtg gatgaaaatg gtgctgctcg
    22441 cgttatcctg agtcagaaaa tcttgaaaca atacaatata accgatgctg aaactgcggc
    22501 cattgtaggt gcacctggac gcattgacag agtgagtctc tggggaattt ttgtcgaaca
    22561 ggctgatggc cactaccgag ttcgcttacg cagtaaagtc catcctatca atgaaattgc
    22621 caaggagcat gatggtggag gccaccctct agcaagtggt gctaattcct atagcctaga
    22681 agaaaacgaa atcatctacc aaaagttaaa aaacttgctt aaaaactgat aaaatacttg
    22741 ccaaactttt cagaatctga tagactagta tagtaacaat ctatggctcg caaagagacc
    22801 atggcagaaa ggaaatattg caaaatgaaa aaagatatcc atccagaata tcgcccagtt
    22861 gtcttcatgg acacaactac tggttaccaa ttccttagcg gttcaacaaa acgctctaac
    22921 gaaacagttg agttcgaagg cgaaacttac ccattgatcc gtgtggaaat ttcatcagac
    22981 tcacacccat tctacactgg acgtcaaaag ttcactcaag cagatggacg cgtggatcgt
    23041 ttcaacaaaa aatacggtct caaataatga taagagaaca gttttggctg ttcttttttg
    23101 tttcttgaaa tcaactgctg ttttcatgtt ccagactcat ctgtaggttc gatttccatg
    23161 ctactaggca ggaaggaaat agctgtttca acacgtccat aatgagctat actattgtca
    23221 cgaaccacac tttcattgat ggtccaagtg gaattcattt tcttaaaagc ttctcggact
    23281 ttttccaaat ctttggaggc aatggcctgc tctaaggttt caaaacgagg acttatactc
    23341 atctgctttc aaaaagcatt ctagtccatc tccgattacc gatggacttt atcacctcct
    23401 tctccagtcc ttgtatgaca tcttgaagtt gattcatgac atcttccaaa gttcgaaagg
    23461 ctttattctt aaatccacgt ttacgaatct ctttccacac ttgttcaatg ggttcatctc
    23521 tggtgtgtat ggaggaataa aggtaaaatc aatattagtc ggaatattta aggtacttga
    23581 tttatgccat atagcattgt ccataacgag taaaaggata agcttgtgaa agctcttcta
    23641 aaaaggcgtt catccacact cctttttata aacctgaaat aaggcatcaa ttgtaacaaa
    23701 ttctcctgcc tctgtagcct tcaaatgacg ggcaagaaag gctttctctt cctcaactgt
    23761 catatatgca tggttacgac caccacgtgt ttcttgaagg agagagtcga gtccgaactc
    23821 ctcatatttt tttacgtttc gccaaatcgt tgtttgatta cagtctaaaa gctctataat
    23881 ctctttataa gatttgccca tcagacgaaa tatagtagat tgaaactaga atagtacacc
    23941 tctacttcta aaacattgtt agaaatcgat ttgtcctgtt cttgtt
//
LOCUS       DAVKHZ010000026        23430 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000026 DAVKHZ010000000
VERSION     DAVKHZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.026"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VWW99_000955"
     CDS             complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VWW99_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9538817.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2238..3107)
                     /locus_tag="VWW99_000956"
     CDS             complement(2238..3107)
                     /locus_tag="VWW99_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9538818.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKATEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3331..3939)
                     /locus_tag="VWW99_000957"
     CDS             complement(3331..3939)
                     /locus_tag="VWW99_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9538819.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(3897..5003)
                     /locus_tag="VWW99_000958"
     CDS             complement(3897..5003)
                     /locus_tag="VWW99_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9538820.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5114..5368)
                     /locus_tag="VWW99_000959"
     CDS             complement(5114..5368)
                     /locus_tag="VWW99_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538821.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5726..6172)
                     /locus_tag="VWW99_000960"
     CDS             complement(5726..6172)
                     /locus_tag="VWW99_000960"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538822.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6245..6570)
                     /locus_tag="VWW99_000961"
                     /pseudo
     CDS             complement(6245..6570)
                     /locus_tag="VWW99_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6804..8522
                     /locus_tag="VWW99_000962"
     CDS             6804..8522
                     /locus_tag="VWW99_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9538823.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            8633..8980
                     /locus_tag="VWW99_000963"
     CDS             8633..8980
                     /locus_tag="VWW99_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9538824.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9259..10095)
                     /locus_tag="VWW99_000964"
     CDS             complement(9259..10095)
                     /locus_tag="VWW99_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9538825.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10108..10737)
                     /locus_tag="VWW99_000965"
     CDS             complement(10108..10737)
                     /locus_tag="VWW99_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9538826.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10747..11388)
                     /locus_tag="VWW99_000966"
     CDS             complement(10747..11388)
                     /locus_tag="VWW99_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9538827.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11534..12763)
                     /locus_tag="VWW99_000967"
     CDS             complement(11534..12763)
                     /locus_tag="VWW99_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9538828.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12753..13919)
                     /locus_tag="VWW99_000968"
     CDS             complement(12753..13919)
                     /locus_tag="VWW99_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9538829.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14063..15076
                     /locus_tag="VWW99_000969"
     CDS             14063..15076
                     /locus_tag="VWW99_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9538830.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15121..15540)
                     /locus_tag="VWW99_000970"
     CDS             complement(15121..15540)
                     /locus_tag="VWW99_000970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9538831.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15551..16957)
                     /gene="atpD"
                     /locus_tag="VWW99_000971"
     CDS             complement(15551..16957)
                     /gene="atpD"
                     /locus_tag="VWW99_000971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9538832.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17043..17921)
                     /locus_tag="VWW99_000972"
     CDS             complement(17043..17921)
                     /locus_tag="VWW99_000972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9538833.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(17937..19442)
                     /locus_tag="VWW99_000973"
     CDS             complement(17937..19442)
                     /locus_tag="VWW99_000973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9538834.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19457..19993)
                     /locus_tag="VWW99_000974"
     CDS             complement(19457..19993)
                     /locus_tag="VWW99_000974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9538835.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(19993..20487)
                     /gene="atpF"
                     /locus_tag="VWW99_000975"
     CDS             complement(19993..20487)
                     /gene="atpF"
                     /locus_tag="VWW99_000975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9538836.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20501..21217)
                     /gene="atpB"
                     /locus_tag="VWW99_000976"
     CDS             complement(20501..21217)
                     /gene="atpB"
                     /locus_tag="VWW99_000976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9538837.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21252..21452)
                     /locus_tag="VWW99_000977"
     CDS             complement(21252..21452)
                     /locus_tag="VWW99_000977"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9538838.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21527..22534)
                     /locus_tag="VWW99_000978"
                     /pseudo
     CDS             complement(21527..22534)
                     /locus_tag="VWW99_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(22979..23377)
                     /locus_tag="VWW99_000979"
     CDS             complement(22979..23377)
                     /locus_tag="VWW99_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9538839.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT         6928 a         5023 c         4265 g         7214 t
ORIGIN      
        1 tatagtttgc tctttgattt tcattgagta ttagttcaat atctaaaaaa agaaccttgc
       61 caagcaagat tcttttagtg tctaacttaa ataattgttc ttctgggaac taaatcgaat
      121 taagcttcga tttctgtaac catacctgaa ccaacagtac gtccaccctc acggatagag
      181 aatgtagtac cttgttctac ggcgattggg tgaatcaact caacgtcgat tgtcacgtta
      241 tcaccaggca ttaccatttc agtacctgct ggaagttcga ttgaacctgt aacgtcagta
      301 gtacggaagt agaattgtgg acggtagttg ttgaagaatg gagtgtgacg tccaccttct
      361 tctttagtaa ggatgtagac ttcacctttg aatttagtgt gtgggttgat tgaacctggt
      421 ttagcgataa cttgtccacg ttcgatttca tcacgttgaa caccacgaag aaggacacct
      481 acgttatctc cagcaagacc ttcgtcaagt tgtttacgga acatttcaac accagtaaca
      541 actgcttttt gagtttcttc tttgatacca acgatttcga tttcgtcgtt gactttaacg
      601 ataccacggt cgatacgtcc tgaagcaact gtaccacgtc cagtgattga gaatacgtcc
      661 tcgactggaa gaagcaatgg tttgtcagtg tcacgttctg gttctgggat atactcatca
      721 actgtgttca tcaattccat aacgatgtct tcgtatttag agtcaccttc aagagcttta
      781 agtgctgaac cttggataac tggaagatcg tcacctggga agtcgtattc tgacaatagg
      841 tcacggattt ccatttcaac caattcaagc aattcttcgt cgtcaaccaa gtcaactttg
      901 ttcatgaaga cgataaggtg tttaacacca acctgacgtg aaagaaggat gtgctcacga
      961 gtttgtggca ttggtccgtc agttgaagct actacaagga tagctccgtc catttgagca
     1021 gcaccagtga tcatgttttt aacgtagtcc gcgtgtcctg gagcgtcgat gtgagcgtag
     1081 tgacgttttt cagtttcgta ctcaacgtgc gcagtgttga tagtgatacc gcgttcgcgt
     1141 tcttctggag cagcatcgat agacgcatag tctttaggtt ggttaactga tgaaggcaag
     1201 cgacgtgcca aaacagttgt gatagctgca gttagggtag ttttaccgtg gtcaacgtgt
     1261 ccgatagtac caatgttaac gtgtggttta ctacgatcgt atttttcttt tgccatttga
     1321 gtaaaagcct ccaataaaat atattttata gatagacagt aggcaataca gtctaacttt
     1381 ccttactatt ttatcaaatt tcagctaaat tgcaagtgtt ttacaacgtt tttgtgaatt
     1441 attcttggta aaaatttaac taattaatag aaagtactaa atagacttat ccttcaagga
     1501 gttttcagca ttatccatcc actcacttgc tgaaaatgta aacagtctct ggtttatata
     1561 gtaaaatgaa acaagaatag ggcaaatcgt tcaggacagt caaatcgatt tctaacaatg
     1621 ttttagaagt agatgtgtac tattctagtt tcaatctact atacaacctc aaaactgtgt
     1681 tttgaacaac ctgcggctag cttcttagtt tgccctttga ttttcattga gtataaaatt
     1741 gcagataaaa cgataacaat cataggactc agcagagtca atgtaggtcg aagaatagcc
     1801 cccctaatcg cttattttat agtaaaatga aatagaaact gaacattttg ttgaggaagg
     1861 taaatcaatt cctcacaatg tttgagaaag aagggaggac tattccaact tcaacccact
     1921 ataaagcaga cttacaagat agggaaccca tctccgataa tattccgata atatatagta
     1981 gaatgaaaac tgcacaattt gattggagaa ttcaaaccaa tttctcacaa tgttttagaa
     2041 acaagggtct attatttcaa ctgcaaccta ctatagtcca ttgaagctgg aatagcccac
     2101 tgagacttct aaaccactgc tggagaggag tttgtttaga gtttctttca tttccctaaa
     2161 aaatcaaaaa gagagaacca gtctgatcct ctcttactta tagatggtgc tcactcaaat
     2221 gttaaaggag ctatttctta gagataaagg aatttgaata cagctactgc cgcaattgct
     2281 gctgcgatag gtgctactac tggtacccaa gaataccacc attttgaatc gcctttatgc
     2341 tcaccaagaa ctgatttggg aaggaaagca tgaaggagac gtggtcccaa gtcacgggct
     2401 gggttcaagg caggtcctgt aggtcctcca agtgatgtta ccaaagccat aacgaggaat
     2461 ccaagtgcca agtgagccac agaaagtcct gaagcagtgt gtggagccac ctgtgcttta
     2521 atagccaagt cagaaaaatc aactgtttgt cctgcttctg ttgccttttg tttcatgaat
     2581 tgaagcactt cagcaccaaa gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca
     2641 aatgaaccaa caaactcatt aatcaaacca ttgacagttg ctgcatagcg actttctttt
     2701 gtaccatggt caatacttga aatagttgag aaagttccca agatgttatt tgggttttca
     2761 gttttcaagt agaatggacg gtatgttgcc acaactaagg cttggccaaa gatagccccc
     2821 aagacttgcg cgataatgta aggtaccact tgtgcccaag ggaaaagacc gctaactgca
     2881 agccctagag tgaaagcagg gttgatgtga ttcccagata cgttaccaaa catcaaggct
     2941 gggatcataa cccccatacc ataaccaaca gcgatgacga tccagccact ttggtgacct
     3001 ttcgtacctt taagttcaac gttggcaact gcaccattcc caagaatgat caaaatggca
     3061 gttcccaaaa attcagtggc atacttcagt gcccatgtga aatccatttg ctagattctc
     3121 cttaaaattt tttagatatt ccttatttta tcaattttta ccctatttag caactgattt
     3181 ctggaaatat ttttaaaaaa ataggattca tagtttccca gttcaacaga cttgtaagcg
     3241 tttttattct ttacgaaaga cctcgttctc aaaaccagga aaaaagccca catctgtgag
     3301 cttttcctgg ttcctagatt ctaaaatcta ttatttttca tcttcttttt tcttgaaaaa
     3361 tcctaatcct agaccagcta acaaggtcat gataccagca ctagccaagg tagcattagc
     3421 ttctgtacct gtgtttggca attcctctct cttacctgtc tcataagtcg gaacttctgg
     3481 gtctggattc actggagttt cagtttttgg agtacctggt tctggagttg gtttatctgg
     3541 tgttggagtc tcttgatttg gtagttctgg gtttggtgtc ggaacacttg gatgctctgg
     3601 atttggttgt gggattggtt tttctggtgt tgataaacgg tcatacctta ccgttatttc
     3661 tttatcacta gagtctgacg taacttcttg tgattcaact gttggaatat ctggatcttt
     3721 gtacttgtca atcttaccag atataacctc gtcccagttt cctgttgtcc attcaccgta
     3781 ggttacaact cccgtgacct tgttctcagt ttttgtacgg cttaaggtta caggttgaac
     3841 aacatcttct tttacatttt ggttcgtaac tttatcaacg taatgaatga tacgcgttat
     3901 agtcttcgtc tcagtagagg ttgctgtttt gggaaccact gtttcctcaa cattctcacg
     3961 gtagtaatag tcaactgttg caccgtcttc tggtacgcat ttgcaggagt tgctaccaag
     4021 gtgtatgttg ttttccttgt gataactcgg tcttctttgt cctcagttgt tgttttccct
     4081 tcaatagttt ttgattctgt ggtatactca gaacctatcg ctaaatcagc ttttataaca
     4141 gactctacca acttctcttg cctaccttct ttatagtaat tggctgttac tgtagcagtg
     4201 gttggcgctt cgctttctct ataaactaag gtcactgttc taccttcgct tacaatattc
     4261 ccagttaaac ttgcagaatt tgtatctgct tctttaaaag tataatattt tccgtcagta
     4321 gtagtcatgc tactgagttt tttatctgtg acattatagc tggtaccaat cagttgtttt
     4381 ttattggtaa tgtaggttcc gtcactttct ttttctccaa ttccagtatc atttttcaat
     4441 gatagcaaac gcccttgttc atcaacatag cgaactttca cattttctga gattagttct
     4501 gccaattctg aggttttttt ctttttcttg atttcttcgg ttattttccc tttctcttct
     4561 tcgggaatat ttagttttgg aatgattttt tcaacaacgg ttcgtgatgg ttccacagta
     4621 tcttggatga ctgaaaagtc agctagaatt gggagattat actgaacacg gtgactttga
     4681 gtgtttactc ctactctttc attattctct gaaaatactt gtacggtata agaaacaaca
     4741 tcttttccta atagaacatc cccagtagag aaatagccgc cttttcctag tttgctatct
     4801 ccagagccca cttctttcct aatcttatca gatagttttt taccagtcag tacattcgtt
     4861 cgcacaatcc ctttgtctac ccctacaaag tgggagaact ttttgaactc ttcagaacca
     4921 gatctagccc aaccattatt aagggcattt gcttttgtat ttgtattctc tctcaaaagt
     4981 ttggcgatta gaattatatt catcggcact tagagttgct gctatatctg actcttgaat
     5041 accaacttcc ttactaccat ttctagcggc agtatatgtg tatttatctg tttctacttc
     5101 tagctgatta tccttaacat gataggtaat tttctcctgg tataagagtc ttcagtagtt
     5161 tatatactct gtcatcatct ttagctttag aagcagctga gtttagccaa gttggggtat
     5221 taggagtatc tttatcagcc tgtttcaaga cattattcaa gtcatttgct agcctattgg
     5281 ttatcctcac tgtgaaagtt ttcttccagt ctgaaccact tttattagac ttagatatgc
     5341 tcatgaaatc ctggtcaaca ttcaacacaa tatcgccttg ttttttagca tcttccgttg
     5401 cttctgcttc aaattgatca tggagttttt gttgctctgg tgtatagttt ttttatttac
     5461 acgatttaat gtactatttt ttattttagt attaagaact ccattaatac gatttttaaa
     5521 ctctgcttca aaaaatccct taatttgttg gtgcgcaagt tcaacattca aacctttctt
     5581 atacaagtct tcattctgat acaagtcttc attctgaacg atttcggcta cacggtgaat
     5641 ggtttgattg tataccgtct tctcttaacc agcagtatcc ttagttttaa tgactactcg
     5701 ataggttcct ttagaaccgt tatagtcatc tagttttaaa aaagcattcc aaacattcaa
     5761 aaatagttgg aacttccctt aatacatccg tcactttctt tttcaagata gcccaagctt
     5821 gctcaatagg attcaaatcc ggtgaataag gtggtagagg taagaaaaag tgtttatctt
     5881 ggatgcagag ttcatccaaa atgttcttgg gatgaaaacc agcattgccc atgacaataa
     5941 catgaggtgt cttcaaagca ggtaggagtt gcgttttgaa ccactccaca aagaaatcgc
     6001 ttgtcatgct tttcttgtaa atcatgggag ctataaactc tccgtctact tgtcctgcaa
     6061 caattgaagt tcgctcaaaa cgacgtccgc taatcttttc atagactttc tcccctctag
     6121 gagcccctgc ataaggacga tagagatagc ggtcgattcc tgtttcatca atataaacag
     6181 gtgctaggtg ctttaaacta ttaaaattct taagaaataa ggctactttt ctgggtcttg
     6241 ttccttaaag ctcgtcatct ttttttaaag tgaccttaat ctgctttaaa gctgcccata
     6301 ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca tctggatgag
     6361 cctctacaaa ggctttcaat tcctctaaag gaatctttcg gtttttgacg actcgctttt
     6421 tcctctctat gtgttcttgt tcacgtcttt cttttcccac gtgaagagag ttctgacgcc
     6481 aacaccaaaa aacttggctg cctcgacatg gctgtgcccc tctttgatgt aatctaatgc
     6541 tcgctgttta aaatcagtac tatatgccat agctttatta taatatgctt gtttaaaact
     6601 aagtaactat atcaatgttt tgttatgaga agacttactt tttttactga aattgaattt
     6661 ttcctagcct caacatcata taaccccaaa attcaatctg acctggtttg aagagatgtt
     6721 cgaagcagat aaggttttac taaaactaaa aaaaatgata taatcaaact gattaaatgt
     6781 tttaaaaata aaggagaatt tgaatgtctt accaagaaaa ttaccagaaa tgggttgatt
     6841 ttgtggagct tcctgactac cttcgtcaag atttggaaaa tatggacgaa aaaactaagg
     6901 aagatgcctt ctatacaaat cttgaatttg gtactgcagg tatgcgtggc ttggttggtg
     6961 ctggtacaaa ccgtatcaac atctacgttg ttcgccaagc tactgaagga ttggctcgtt
     7021 tgattgagtc aaaaggtgga aacgagaaag aacgcggtgt agcaattgcc tacgatagcc
     7081 gtcacttctc acctgagttt gcctttgaat ctgcggcagt tcttgctaaa cacggcatca
     7141 aatcttacgt atttgaaagc cttcgtccga ctccagaact atcatttgca gttcgtcatc
     7201 tcaactgttt cgcaggtatc atggtcacag ccagccacaa ccctgctcca tttaacggtt
     7261 acaaggttta cggtgaagac ggtggacaaa tgcctccaca cgatgcggac gctttgacta
     7321 cttatatccg tgcaatcgaa aacccatttg cagttgaagt tgctgatgtg gaaactgaaa
     7381 aagcttctgg cttgattgaa gttatcggcg aagctgttga cgtagaatac cttaaagagg
     7441 ttaaggacgt aaacatcaac ccagccttga ttgaagaatt tggtaaagac atgaagattg
     7501 tctacacacc acttcatggt actggtgaaa tgttggctcg tcgtgctctt gcccaagcag
     7561 gatttgactc tgttcaagtt gttgaagcgc aagcaactgc tgacccagac ttctcaactg
     7621 taacatctcc aaacccagaa agccaagcag cctttgccct tgctgaagaa cttggtcgtc
     7681 aagttggtgc agatgttctt gtcgcaactg acccagacgc tgaccgtgtt ggtgttgaag
     7741 ttcttcaaaa agatggtagc tacctcaacc tttcaggtaa ccaaatcggt gctatcatgg
     7801 ctaaatacat cttggaagct cacaaaaacg ctggaactct tcctgaaaat gccgctctct
     7861 gcaaatctat cgtttcaact gacttggtaa cgaagattgc tgaaagctac ggcgcaacta
     7921 tgttcaacgt cttgacaggt ttcaaattta tcgctgaaaa aatccaagaa ttcgaagaaa
     7981 aacacaacca cacttacatg atgggatttg aagaaagctt cggttacttg attaaaccat
     8041 ttgtacgtga taaagatgcc atccaagccg ttcttgtcgt tgctgaactt gctgcctact
     8101 accgttctcg tggtttgaca cttgctgacg gtatcgaaga aatctataaa gaatatggct
     8161 actacgcaga aaaaacaatc tctgttactc tttcaggtgt cgatggtgct gaacaaatca
     8221 aagcgattat ggctaaattc cgcaacaatg ctccaacaga atggaacgca acagctatca
     8281 ctgtcgtaga agacttcaag gcacaaactg ctactgttgc tgacggtact gttacaaact
     8341 tgacaactcc tccaagtgat gtgttgaaat acacacttgc tgacggttca tggattgccg
     8401 ttcgcccttc aggtacagaa ccaaaaatca agttctacat tgcagttgta ggggaaacca
     8461 acgaagaatc acaagctaag attactaaca tcgaagcaga aatcaatgca tttgtaaaat
     8521 aaagacctat aaaagatgag accaaccaac ctcatctttt ttcgtatcaa atctaagcaa
     8581 ttttagaaac tttatggcat actagactta tcaatgaaag cgaggtaatc tcatggaaca
     8641 atttttagat aacatcaaag accttgaagt cactacagtt gtgcgtgcgc aagaagctct
     8701 tgataaaaaa gaaactgcaa ccttctttat cggtcgcaaa acttgccctt actgccgtaa
     8761 atttgcaggt acattgtcag gtgtcgtagc tgaaaccaaa gctcacattt acttcatcaa
     8821 tagtgaagaa ccaagccaac tcaatgattt gcaagcattc cgctcacgtt atggaatccc
     8881 aactgtacca ggttttattc atattacaga tggacaaatc aacgtccgtt gcgactcttc
     8941 aatgtcagca caagaaatca aagatttcgc aggattgtaa aaaatcagct aacaagtctt
     9001 aaaatcacaa aaacgcataa tgtaaggtgc aaaaaccttg atattatgcg ttttattgtt
     9061 cctaaaacag ttacatcagc atttcattac agaacatttt aattttggaa gtcaaatcaa
     9121 tttttaacaa tgttttagaa atcacagtgg gctattccat attcaattat ccagattcaa
     9181 tcgctattat cgttttccta gattacaaaa aaaactacgt tttgctaatc aaaacgtagt
     9241 ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta catcttctcc
     9301 aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag aaaaagcttc
     9361 atttatcttc ttaaccaagg ttgtatcttc cttacgggat ccaaccgcaa aagcttctgt
     9421 ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt ctaaataata
     9481 gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca aatcaatcaa
     9541 ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat tcttcaaaat
     9601 ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc ctaatgtctt
     9661 tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca atacctgctc
     9721 attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg tagcggaata
     9781 gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca aatcccaatc
     9841 aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag ctaaatcaat
     9901 atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa aagtactatc
     9961 aaatccaata gtaatagact tgttagactg gtactttgac caattatctc cactagtttc
    10021 gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga ctaatacaag
    10081 catccatttt ttcatccatt tttcctccta tttaggttct actttcaata atacatctgc
    10141 gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc caagttccct
    10201 attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg cagaagttgg
    10261 ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag ccacccgctg
    10321 cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta gtcctaactg
    10381 ttccaagagc ccactcgcct tcttctcagc ctcttcctgc ttcattccca tggtcttcac
    10441 aggcgataaa gtcaaatttt ccagaactga tagatgagga aatagttgaa aatcttggaa
    10501 gacaaatccc agtaggttgc gcttctgcaa ttcatccagc tctaaaggtt gtccattata
    10561 aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa gagttgtctt
    10621 acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac ttagactgaa
    10681 attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt ctaacatggc
    10741 agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa ttgtcacaat
    10801 cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac tagcatcgcg
    10861 gttagcagct gttcgactag ccaagataag gtctgaaatt ccgagagcat agaccaaaga
    10921 agtatccttg accaaactca taacttcatt aaagacacta ggaagaacaa tcttggtcac
    10981 ttggggcaag ataatatagc gcactctgtc aaaagggcta aactttaaga ccttggcagc
    11041 ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg caaagtaagc
    11101 tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac gaatcccaat
    11161 acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc ctcgcataac
    11221 ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca aggcaaaggc
    11281 aatcaaaacg cccaagggaa tcgaaaatag caagaccagt gcaaagacct gtacagtcgt
    11341 gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac tgcacctcct
    11401 aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt tttgtaaaaa
    11461 gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta cagttctata
    11521 tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca tctggaacca
    11581 gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat tcacgcaaga
    11641 gatagatata gtgttccaga aattctggat tgtccttcaa atctcctgtc aactcttgat
    11701 aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca atcatccact
    11761 tggtcaaaag attttctggc tcctcactct gcaagtctag aatatcctca taacgctcct
    11821 gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca gcgtcttctt
    11881 ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa gaaaattgtg
    11941 aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc ttagcgatac
    12001 gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca tactcatagc
    12061 cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct ttttgatatt
    12121 cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg tattctaact
    12181 ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga gcataggcaa
    12241 agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga ttatctaact
    12301 gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac tctgccaaac
    12361 ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc tcacgtgcca
    12421 catctgtcaa accttccagc tggtaaaggt ctgccttcaa aaccaaagac gagacatacc
    12481 agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt tgaccatcct
    12541 cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca attttcaggt
    12601 aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc aactcataca
    12661 gaagattact tgaatcattt tctaaagctt tggcgaaata atgctcagcc ttagttaaat
    12721 cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac ttcctcaccc
    12781 tctaattcat atagaccact gactacctta taccattcga aaatggctga aatgacaacc
    12841 ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa catagatcct
    12901 gaagttaaca aaacaaagag aacattaata gggtggatgt ttaattgact tcccaaaatc
    12961 aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat cactactttc
    13021 aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat ggctagaaag
    13081 ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt aaccgcatag
    13141 cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat agccactgtc
    13201 acttgccctc gaacatagtt ggacaactgt tgattcacat ctgatagaac ttgtccaaca
    13261 ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc tttcccatca
    13321 cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac ttgagaagcc
    13381 ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact tgccaaaact
    13441 gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa atcatctggc
    13501 agatgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta aacaggaacg
    13561 tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc caagccccaa
    13621 atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg attaacctta
    13681 tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc agacaaaatg
    13741 actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa actaatctta
    13801 cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt aattgccttg
    13861 ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt ttgctccata
    13921 aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata actctttttt
    13981 aaagtgctta catctaattg gatatcttta atttacttgt ttttgtgata taatagtctt
    14041 atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt atacacgtcg
    14101 tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc tttcaaatct
    14161 agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc accaacattt
    14221 atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa ctgacgggac
    14281 tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg tcgatgaaaa
    14341 gcgtgatttg gtttacgcag ctaactatca caagggacaa gtccttgttt ataaacgcca
    14401 ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag gtccacatga
    14461 aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact atctagtgac
    14521 ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag gaaaattatc
    14581 taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca ttttccataa
    14641 ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag ttttaatcta
    14701 cgatggcgtt ggcgaatttg aacgtatgca ggtcatttca actttaccgg aagcttacga
    14761 aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc tctacgcttc
    14821 taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta gcttagagtt
    14881 attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga cacccgacca
    14941 aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta aacgtaattg
    15001 tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag cagtctgcat
    15061 ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt tcattttttc
    15121 ttatagtcta tttccgacat taatacggtt aatggcacgt tgcaaagcaa tcttagcacg
    15181 acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt ctgcacgaag
    15241 tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag agtcagcgac
    15301 gattgtaatc atatcattgg caatttcaat aacgccaccg tttactgcaa tccagttcac
    15361 gtgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa ccgcaatcat
    15421 attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg atacatagct
    15481 ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta actgagccat
    15541 aggtcacctc ttaaaatccc attttttcag cttttgcaat cacatcttcg atagaaccta
    15601 caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt tccttaaagc
    15661 cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat tgttccgcaa
    15721 cattgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc aaggtctttt
    15781 cttcatcaga aagctcatcc ataccaagga tagcaatgat atcttgcaat tcatggtaac
    15841 gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca acgatttcag
    15901 gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata cccaattgta
    15961 ccaatttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt gctggcgctg
    16021 ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca gaacccttct
    16081 tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt ggttggtaac
    16141 caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc tgagtgaaac
    16201 ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg aagtattcag
    16261 cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca ttcatctgac
    16321 caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag taaaggtcat
    16381 tcccctcacg agtacgttcc ccaacaccag taaatactga aataccaccg tgctcttggg
    16441 caatgttgtg aatcaattct tggattaaga cggttttacc aactccggca ccaccgaaaa
    16501 gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg atccctgttt
    16561 caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta tgaattggct
    16621 gacgctctgc gtcttctgta aaaggagctt ccaaatcaat ggtatctccc aaaacgttga
    16681 agacacgtcc caaagtttct ttacctactg gtacagagat tggacgacct gtgtccaata
    16741 cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga accataccat
    16801 ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca tttttgtaga
    16861 cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa acgtctacaa
    16921 cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat tctatataag
    16981 atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag ctggactaga
    17041 gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt aatcgccgcc
    17101 tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc attatctgtc
    17161 gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc gataatggca
    17221 ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc ttctcggctg
    17281 gtttccaatt caaaagtcaa gctgtactct tcatccgcct catttggatc caagtcaaca
    17341 atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg gtggttgtag
    17401 caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt tgaaataatc
    17461 ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata aagtggttga
    17521 ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat ttcaaaacct
    17581 ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat agaggaatta
    17641 taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt cttcacagaa
    17701 cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag gatatctgtc
    17761 aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc ttcagagcga
    17821 cctaacttag cagccgatac catttgcata gcattagtga tttgactcgt attttttgtt
    17881 gaggcgattt ttgttttaat atcatttaga gacactgcca tctgacacct ctattcttat
    17941 tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc ttcttctggc
    18001 aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc aaagaaggca
    18061 tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa gaaaccatgt
    18121 gtcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg aacaggttgt
    18181 ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc tgcgtccaag
    18241 tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc gatacgaagt
    18301 gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg agatacagat
    18361 gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc aaggaagatt
    18421 tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc tcctgcttgt
    18481 gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga aactttagct
    18541 gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc ttcacgacct
    18601 ggaggacgac gaagcaagag cgatagttca cgataagcta ccgcttgttt tgatagatca
    18661 tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat agcaacccca
    18721 gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt cacaacgatt
    18781 gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac tgttgattct
    18841 ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt caagattgta
    18901 tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc acgttgacca
    18961 cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg ttctgaaaca
    19021 gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt atcagtgtgg
    19081 atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc aatcagactt
    19141 tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc ttcacggata
    19201 tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt ttgagccata
    19261 ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc aaggccatga
    19321 gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac atcaaaattg
    19381 ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc gttaattgcc
    19441 aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt tgtttaatac
    19501 tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga ctttcatcga
    19561 tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa gggagcaaac
    19621 gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca aatcgatttg
    19681 tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga tttgctctgt
    19741 gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa tcttggaaaa
    19801 aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa aaagaaggca
    19861 gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata cggtcttctt
    19921 ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt acctttactg
    19981 ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga gtgctttatg
    20041 ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg tcaaatctgc
    20101 gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt cttgattggc
    20161 tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag ccttactttg
    20221 ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc taccagccaa
    20281 ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt cagctctgtc
    20341 aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat tagaccatgc
    20401 aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa caaaattacc
    20461 aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct tcaatattaa
    20521 tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg atgcaggaaa
    20581 taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga taccaataaa
    20641 tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca aagatattcc
    20701 caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata ttcatcggta
    20761 tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga cgacgaacgc
    20821 tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac tgtaaattag
    20881 cggttggcga actccaccag ttagtcccat cgatcgtttg aatctttgtc attaagccaa
    20941 ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgag taatctttca
    21001 tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag acatactcaa
    21061 gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa taaataaaga
    21121 caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg aagataacag
    21181 gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc ttttcttctc
    21241 gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa agttccttca
    21301 ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc tggttggcgt
    21361 gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac gcccatacag
    21421 gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa ataaaattta
    21481 cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat tggaagcgtt
    21541 taataaaaat tatttccatt ttgctaaatt tcaagttcaa gttagccatc cagaagtctt
    21601 ctctgggtga cttgtagtcc aagcattttt taggatagtt gttaatccac ttttcgatga
    21661 atgcgacttc tttgggagtc attttcttgg ttcccttagg taaccatcta cgaatgagcc
    21721 tgttgtgatt ctcattagtt cccctttccc aagaggcata gggatgtgca taataaatgt
    21781 gctcctcaga aaatacatta gacaagcgat tgaattccgt tccattatct gccgtgatgg
    21841 aaagaatctt gtgttgtttt aagatgagtt ttagagcctg attgaccacc tcagcacttt
    21901 tatttggaat caatcggatg atctgatgtc tactttttcg atccgtcaag acaagcaagc
    21961 agtagttttt cgctctcgta agtagaaccg tatcaatctc ataatgccca ttctccaagc
    22021 gaaaattgat agcttcaggc cgctgttcga tggattgacc agcaggttta aagttggtgc
    22081 tggcctgttt cttaagcgct tttccttttc tagggtaaag cagatcctgc ttgctttacc
    22141 ccaattttcc atgatgaatc caatagtaaa tggttgaaat tcccacgtta acccctttag
    22201 ccataaccat catttcaggc aaaaattttt ggttatgata gtggagaatc ttttccttta
    22261 gttccttggt cacgcttgat ctcttgaccg agtgcttgcg attattttca taagactgtt
    22321 gagcatagtc ggcagaataa acctctttga agcgcccttt tccaagacat tttcggactg
    22381 tcctacgctt gattttagtg tggatagttt gaggagcttt tccaagtaga gaggcaattt
    22441 ctctatttga ttttccttct tttttccatc tttcgattaa gcgacggcta tcgattgtca
    22501 aatgtttacc ttttgtagta taattgtctt gcatttctgt gcctttcttg tgtttgtggt
    22561 tgaacaacaa gtataacaca gaggtgtttt cttatgccta caagagcttt cattatttcc
    22621 attttctttt ggatttcact ctattctgaa aaacttgtgt atatttactg aagctagcaa
    22681 gtcttacctg taaatttaat gaaagcaaca caaaatccga gaggggaatc tcggattaat
    22741 agatagagag tttttagttt aaataaattg tttaaaatat caacaacatc acttcttttc
    22801 ttaacctgat aagtcttgat tcctaatttt ggggctacga ttatattgtc ctcaatatcg
    22861 tctagaaaga cacaatttct aggttataac tggtatttat caatagttac tcatatacat
    22921 cagtccacct ccatacttat gtgcgagcct ctctttgtat tatacctcca tactcacctt
    22981 acagattctt ttggtaataa tatctttgcc taatgtagag atagtcttgc aaagaaaaaa
    23041 cttccttgta gccatgtttc tgataaaagt ccggtgcctg gaactggtaa gtattgacaa
    23101 aggcaaaaca acaatttcga ttcttagctt cactttctgc ctgttgcaat agttttgaac
    23161 cgattccttg ccctcgcagt tcctctttta caaacaaata ctcgatttct agccaatttc
    23221 caaaagtctc tgctatcaaa cctgccagga gattgccctt ttcatcttcg acataaagat
    23281 taagtggctc actttcagcc tcttctcttt ttgaacggtt ataaacacga atcagattcc
    23341 ctatttcttg cgatttatgt gattccttat tttccaatct aaagtatagt gaaatgaaat
    23401 aaaacatgcg caaatcgatt aaggaattta
//
LOCUS       DAVKHZ010000027        22367 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000027 DAVKHZ010000000
VERSION     DAVKHZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.027"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            348..1391
                     /locus_tag="VWW99_000980"
     CDS             348..1391
                     /locus_tag="VWW99_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9538840.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1558..3645
                     /gene="topA"
                     /locus_tag="VWW99_000981"
     CDS             1558..3645
                     /gene="topA"
                     /locus_tag="VWW99_000981"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9538841.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3866..4225
                     /locus_tag="VWW99_000982"
     CDS             3866..4225
                     /locus_tag="VWW99_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9538842.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4318..4950
                     /locus_tag="VWW99_000983"
     CDS             4318..4950
                     /locus_tag="VWW99_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9538843.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQAEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4994..5650
                     /locus_tag="VWW99_000984"
     CDS             4994..5650
                     /locus_tag="VWW99_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9538844.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5669..6763
                     /locus_tag="VWW99_000985"
     CDS             5669..6763
                     /locus_tag="VWW99_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9538845.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6775..7812
                     /gene="leuB"
                     /locus_tag="VWW99_000986"
                     /pseudo
     CDS             6775..7812
                     /gene="leuB"
                     /locus_tag="VWW99_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7809..8078
                     /locus_tag="VWW99_000987"
     CDS             7809..8078
                     /locus_tag="VWW99_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9538846.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            8082..8441
                     /gene="leuD"
                     /locus_tag="VWW99_000988"
     CDS             8082..8441
                     /gene="leuD"
                     /locus_tag="VWW99_000988"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9538847.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8660..8803
                     /locus_tag="VWW99_000989"
     CDS             8660..8803
                     /locus_tag="VWW99_000989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538848.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            9064..9225
                     /locus_tag="VWW99_000990"
     CDS             9064..9225
                     /locus_tag="VWW99_000990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538849.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
     gene            9389..11320
                     /locus_tag="VWW99_000991"
     CDS             9389..11320
                     /locus_tag="VWW99_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9538850.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCLYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            11307..12623
                     /locus_tag="VWW99_000992"
     CDS             11307..12623
                     /locus_tag="VWW99_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9538851.1"
                     /translation="MQITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            12798..13784
                     /locus_tag="VWW99_000993"
     CDS             12798..13784
                     /locus_tag="VWW99_000993"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9538852.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVSQLKADTPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            13848..13919
                     /locus_tag="VWW99_000994"
     tRNA            13848..13919
                     /locus_tag="VWW99_000994"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13881..13883,aa:Arg,seq:ccg)
     gene            14102..14800
                     /locus_tag="VWW99_000995"
     CDS             14102..14800
                     /locus_tag="VWW99_000995"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9538853.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            14791..18330
                     /gene="smc"
                     /locus_tag="VWW99_000996"
     CDS             14791..18330
                     /gene="smc"
                     /locus_tag="VWW99_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9538854.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKDNKAELDSTEEELAQVQELLTSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLDSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTDLLNQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVYNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18327..19121
                     /locus_tag="VWW99_000997"
     CDS             18327..19121
                     /locus_tag="VWW99_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9538855.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGNKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            19121..19939
                     /locus_tag="VWW99_000998"
     CDS             19121..19939
                     /locus_tag="VWW99_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9538856.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            19943..21220
                     /gene="ftsY"
                     /locus_tag="VWW99_000999"
     CDS             19943..21220
                     /gene="ftsY"
                     /locus_tag="VWW99_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9538857.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYKKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGEKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21272..22367)
                     /locus_tag="VWW99_001000"
                     /pseudo
     CDS             complement(21272..22367)
                     /locus_tag="VWW99_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7060 a         3867 c         5014 g         6426 t
ORIGIN      
        1 gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa
       61 tggttattat aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctcttcaaac
      121 tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc tacggttagc ttccgagttt
      181 gattttcatt tactagaaat gaaactgatg agagatatca gtagacattt gagtcaggat
      241 attatggaaa atgataaaaa gagctcgtga gattggcata tcagactact aaagtattga
      301 gtttgttagg attttagcga ctagttagct gggaaaggaa gatatttgtg acaaataata
      361 aactgtattc gttgatagaa tttagaaata aaatatatga agaattagaa ctttccagaa
      421 gtgatttagc gattttacta tgtgccatgc ttatcgcctc tatcggatta aatatggatt
      481 cgactcccgt gattattgga gccatgttaa tctctccttt gatgacacct attctgggag
      541 tggggctctc tctagctata tttgatttta aattgttaag aaaatctttt aaaatattag
      601 ctattcaaat tcttgccagt ctaatagctt caacacttta tttttatctt tctcccattt
      661 cgtatgctag ttcggagatt gttgctagaa cctctccgac tatttgggat gttctcattg
      721 cttttgtagg agggatagca ggtatcattg gtgctaggaa aaaagagacc aataatattg
      781 ttcctggtgt tgctattgca accgccttga tgcctcctct ttgtacagta ggttatgcta
      841 ttgcttctgc taatctaaaa tttatcatag gctcctctta cctattcctc atcaattgta
      901 gctttattgt cattgcgact tatataggtg ttaggttgat gatggttaag aaacattatt
      961 ttaaagataa tgaagaagac tctaaaatgc gtaggatttt gcttctagtt gctgttttgc
     1021 tgatgattcc gagtttcatc tctgcaacga ctttagtgag agaaacgttg aaaaaagagt
     1081 cccttaagaa atttatatca gagcagtttc aggggtataa tattttgaaa aaaacctatt
     1141 ctaaaaagac tcatacccta aagctaacca tttcaggaaa ttatttgaca gaagaagaac
     1201 tcgatatgat ttcaagtaag agaggtgact atggtttaag tgatgtttct gttcaagttt
     1261 cacaattgtc tgattcagaa caacttagta aggaagagct ggtggagtat ttcttccagt
     1321 atatcaagga taaggaagca aaagaaaagg agaaagccaa taaatttgat acagagtctg
     1381 aggagcaata atttcttgag aatagctggt ttctctcgtg agtcttctat gtatatcaaa
     1441 ggaagactga ggttttaagt ataaaacttt tcttctatta tagtagctaa gtcttgacag
     1501 ggttcaaaaa atggtttaca ctttataaag tttattactt tgaaaaggtg tgatactgtg
     1561 gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa atctagtcat cgtggagtcg
     1621 cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa actacaaggt tttagccagt
     1681 gtcgggcata tccgtgattt gaagaaatcc agtatgtccg tcgatattga aaataattat
     1741 gaaccgcaat atattaatat ccgaggaaaa ggccctctta tcaatgactt gaaaaaagaa
     1801 gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg accgtgaagg agaagcgatt
     1861 tcttggcatt tggcccatat tctcaacttg gatgaaaatg atgccaaccg tgtggtcttc
     1921 aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag aacctcgtaa gatcgatatg
     1981 gacttggtcg atgcccaaca agctcgtcgg atcttggatc gcttggtagg gtattcgatt
     2041 tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag caggtcgcgt tcagtccatt
     2101 gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg ccttccagcc agaagaatac
     2161 tggacagttg atgctgtctt taaaaaggga accaaacaat ttcatgcttc cttctatgga
     2221 gtagatggta aaaagatgaa actgaccagc aataacgaag tcaaggaagt cttgtctcgt
     2281 ctgacgagta aagacttttc agtagatcag gtggataaga aagagcgcaa gcgcaatgct
     2341 cctttaccct ataccacttc atctatgcag atggatgctg ccaataaaat caatttccgt
     2401 actcgaaaaa ccatgatggt tgcccaacag ctctatgaag gaattaatat cggttctggt
     2461 gttcaaggtt tgattaccta tatgcgtacc gattcgactc gtatcagtcc tgtagcgcaa
     2521 aatgaggcgg caagcttcat tacggatcgt tttggtagca agtattctaa gcacggtagc
     2581 aaggtcaaaa acgcatcagg tgctcaggat gcccatgagg ctattcgtcc gtcaagtgtc
     2641 tttaatacac cagaaagcat cgctaagtat ctggacaagg atcagcttaa gctatatacc
     2701 cttatctgga atcgttttgt ggctagccag atgacagcgg ccgtttttga taccatggct
     2761 gttaaattgt ctcaaaaagg ggttcaattt gctgccaatg gtagtcaggt taagtttgat
     2821 ggttatcttg ccatttataa tgattctgac aagaataaga tgttaccgga catggttgtt
     2881 ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac atttcaccca accgcctgcc
     2941 cgttattctg aagcaacact gattaaaacc ttagaggaaa atggggttgg acgtccatca
     3001 acctacgcgc caaccattga aaccattcag aaacgttatt atgttcgcct ggcagccaaa
     3061 cgttttgaac cgacagagtt gggagaaatt gtcaataagc tcatcgttga atatttccca
     3121 gatatcgtaa acgtgacctt cacagctgaa atggaaggta aactggatga tgtcgaagtt
     3181 ggaaaagagc agtggcgacg ggtcattgat gccttttaca aaccattctc taaagaagtt
     3241 gccaaggctg aagaagaaat ggaaaaaatc cagattaagg atgaaccagc tggatttgac
     3301 tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc gttttggtaa attctacgct
     3361 tgtagcaatt tcccagattg ccgtcatacc caagcaatcg tgaaagagat tggtgttgag
     3421 tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa ccaagcgtaa tcgcctattc
     3481 tatggttgca atcgctatcc agaatgtgaa tttacctctt gggacaagcc tgttggtcgt
     3541 gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag tccgtggtgg tggcaagcag
     3601 gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta aataagagga ggggcccgaa
     3661 agtaaatttc aggctccttt tggttagaac ttgacaaaaa acatcatttt atgcgaaact
     3721 agaagaagat tattttatac tcaatgagaa tcaaagagca aattagaaag ctagtcgcag
     3781 gttgctcaaa gcactacttt gaggttgaag atggaagctg acctggtttg aagagatttt
     3841 cgaagagtat taactaggag aagttatgcg tattatctat ctaattattg gttttttatc
     3901 gctgaccttg gctattgttg gggttgtttt acccttgttg cctacaacac ctttcctttt
     3961 gttgtctatt gcttgtttct ccagaagttc caagcgattc gaagattggc tttatcatac
     4021 caagctctat caagcatatg tagctgattt tcgtgagacc aagtctattg cgcgtgaacg
     4081 aaagaaaaaa atcatcgtct ctatctacgt cttgatggga atttctattt attttgcacc
     4141 tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc tttattactt attatctctt
     4201 caaggtcatt ccagacaaag aatagttaaa acagtagtta tttgccttga taaaattgaa
     4261 agcatattca taacaatatg atataataaa attgaagtaa tattcaagga gaatcaaatg
     4321 atttacgaat tttgtgctga aaatgtgact ttacttgaaa aagcgatgca ggctggagct
     4381 cgtcggattg aactctgtga taatctagca gttggtggga caacacccag ctatggagtg
     4441 actaaggcag cggttgaact ggcagctaac tacgatacaa ccatcatgac catgattcgg
     4501 ccacgtggtg gtgactttgt ctataatgac ctagaaattg ctatcatgct agaagacatt
     4561 cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg gagctttaac tgctgataaa
     4621 aagttggata agcctaatct ggaaaagtta attgctgcat caaaaggaat ggaaattgtc
     4681 tttcacatgg cctttgatga actaagtgat gaagatcaag cggaagctat tgactggctc
     4741 agtcaagccg gtgtcactcg tatcctaact cgtgctggtg tgtctggcga ctccttagaa
     4801 aaacgttttg ttcactatca cagaattttg gagtacgcta aaggtaaaat tgaaattcta
     4861 ccaggtgggg ggattgacct tgaaaaccgt caaaccttta tcgaccaggt gggggtaaca
     4921 caattgcatg gtactaaggt tgttttttaa aaaatagaaa ggaactgcta gctttgggta
     4981 gcagttttca cttatgtttg aaatttttaa atcctatcaa tttaatcaag aaaaggctca
     5041 tgattatggt tttatagaaa atagcgaagt ctggacatat agttgccaga ttttgcaagg
     5101 tgactttgtc atgactgtgt ccatcactgc tgataatgtg aactttcaag tctttgacca
     5161 agagactggt gacctctatc ctcacgttta tatggaaagc atgaggggaa gttttgtcgg
     5221 aaatgtccgt gaggcttgtc tggagattct ttaccagatt cggaaggctt gttttgatgt
     5281 gcaagatttt atctgtcatc agactaagcg tatcatgact caagttcagg aaaagtatgg
     5341 aaaccagttg gagtatctgt gggaaaaatc gcctgataca gctgtattgc gccatgaagg
     5401 caatcaaaag tggtatgccg tcttgatgaa aatctcttgg aataagctgg aaaagggcag
     5461 agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa gtagctaatt tgctttcaca
     5521 aaaggggatt tatccagcct tccatatgag caagcgctac tggattagtg tgtcccttga
     5581 tgatacttta tcagatgaag aagtactgga attgatagaa aaaagttgga acttaacctc
     5641 taaaaaatga aatattttaa taattttcat gaactttcaa ttagctaaat attctttact
     5701 gaagagattt ttagaaaata taggatttac cacactagag gaatatggtg ccatcttcaa
     5761 atacctgatt gagaatgtca agacggatcg tcagatcatc tattcgcctc actgtcatga
     5821 tgacctcgga atggcagtgg caaatagcct tgctgctgtc aagaatggtg caggacgtgt
     5881 tgaagggact atcaatggta ttagggagcg agctgaaaat gctgctttgg aagaaattgc
     5941 agtggctctc aatattcgcc aagattacta ccaagtagaa accagtattg tcctaaatga
     6001 gaccatcaat acgtcagaaa tggtttctcg cttctctggt attccagttc ctaaaaacaa
     6061 agccgtcgtt ggtggcaata ccttctccca cgaatctggt attcaccaag atggagtcct
     6121 taaaaatcct ctcacttatg agatcatcac acctgaattg gttggtgtta agattctgct
     6181 tggaaaatta tctggtcgcc atgcttttgt tgagaaactg agagaattgg ccctagattt
     6241 tacagaagag gatatcaaac cactctttgc taagttcaag gcactggtcg ataagaagca
     6301 agaaatcaca gatgcagata ttcgagcttt ggtagctgga accatggttg aaaatccaga
     6361 aggcttccac tttgatgatt tacaacttca aactcatgca gataatgaca ttgaagcgct
     6421 cgttagccta gccaatatgg atggtgagaa agtcgaattt aatgcgacag ggcaaggttc
     6481 cgttgaagca atctttaatg ctatcgataa gttctttaac caatctgttc gtttggtgtc
     6541 ctacactatc gatgcggtaa cagatggaat cgatacccag gatcgggttt tggtcactgt
     6601 tgaaaacaga gatacagaaa ccatctttaa tgcagcaggg cttgattttg atgtgttgaa
     6661 ggcttctgcc attgtctata taaacgctaa tacctttgtt caaaaagaga atgcaggtga
     6721 gatgggacgc agtgtttctt accacgatat gcctagtgtg taaaggagaa ggctatggca
     6781 aagaaaatag tagctctagc aggagacgga attggcccag aaatcatgga ggttggttta
     6841 gaagttctgg aggctctagc tgaaaaaaca ggttttgact atgagattga cagacgaccg
     6901 ttcggaggtg cagatattga tgcagcatga cctcccttac ctgatgaaac ccttaaggca
     6961 agtagggaag cagatgctat cctactagta gctatcggta gtcctcagta tgatggagca
     7021 gtggttcgcc ctgaacaagg cctgatggct ctccgtaagg aactcaatct ttacgctaat
     7081 attcgtcctg taaaaatctt tgacagtctc aagcatttgt caccactcaa actggaacga
     7141 attgctggtg tagactttgt cgtggtgcgt gaattgacag gcgggattta ctttggatat
     7201 catattcttg aagagcgcaa tgcgcgtgat atcaacgact atagctatga ggaagtggag
     7261 cggattattc gcaaagcctt tgaaattgca agaaatcgca gaaaaatcgt tactagtatc
     7321 gataagcaaa atgttctagc gacctcaaaa ctctggcgga aagtagctga ggaagtcgca
     7381 caggatttcc cagatgtaac cttggaacat cagctggtag actcagctgc tatgcttatg
     7441 attaccaatc ctgctaagtt tgatgttatt gtaacggaga atctttttgg agatatttta
     7501 tctgatgaat caagcgtctt atctggtaca cttggggtta tgccatcagc cagtcattct
     7561 gaaaatggac caagtctcta tgaacctatt cacggttcag cacctgatat tgcaggtcaa
     7621 ggaattgcca atcctatttc catgatttta tcagtttcca tgatgttgag agatagtttc
     7681 ggacgttatg aggatgcaga gcgtatcaaa cgtgctgttg agacaagtct ggcggcagga
     7741 attttaacga gagatatagg aggtcaggct tcaacaaagg aaatgacgga agctattatt
     7801 gcaaggttat gaagttagac gaaaaaatta ctctagtcct tttgatttgg aatgtcatca
     7861 ttttcttgat ttatggtatt gacaaatcta aggcaaggag aagagtttgg cgcatcccag
     7921 agaaaatctt acttatttta gcctttactt ttggtggttt tggtgcctgg ctagcaggaa
     7981 tcatctttca ccacaagact cgaaaatggt actttaaaat agtttggttt cttgggatgg
     8041 tgaccacact agtagcctta tattttattt ggaggtaatg gatggcaggg tcttcgaggg
     8101 aatacgctgc ttgggctcta gcggactatg gttttaaggt cgtgattgca ggatctttcg
     8161 gtgacattca ttacaataat gaactcaata atggcatgtt gccaatcgtt cagcctagag
     8221 aggttagaga gaaactagcc cagctaaaac caaccgacca ggtaactgtg gacttggaac
     8281 aacaaaaaat catctcacca gttgaagaat tcaccttcga gatagatagc gagtggaaac
     8341 ataaactcct aaatagtttg gatgatatcg gtattacctt gcagtatgaa gagttgattg
     8401 ctgcttatga aaaacaacga ccagcctact ggcaggatta gaaaaaatag aaaaggagat
     8461 atagtaaact gaaataagat gtaaacaaat gaattggagc ttaacatcca tttccagcaa
     8521 ttttttagaa actacagtgg actattctgg attcaacaca ttataaaatt atgacaaaac
     8581 acattcacaa gaaggctacg acattttaaa aggtgagggc ggatgtatcg tttgccctac
     8641 taaagttggt tacattatca tgaccagtga caaggcagga cttgagcgta agttcgcagc
     8701 caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc ggtagcatgg atgaactttg
     8761 cgctttagcg caactcaacc cagaaattga agcattctac taaaaacatt gggatgaaga
     8821 tattcttctt ggttgtatcc ttccttggaa accagaagcc tttgaaaaac tcaaagcata
     8881 cggggatggc cgtgaagaac ttattactga tgtacgtggt actagctgtt ttgttatcaa
     8941 gtttggaaaa gcaggtgaac aattggctgc caagctttgg gaagaaggta aaatggtcta
     9001 cgcctcatct gcttcaatga caaaacgatt gaaactcgct atgagcaagg tgtaatggtg
     9061 tctatggtcg ataaggacgg caaactcatc ccagaacaag gaggagcacg ttcaacttca
     9121 ccagctccag ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca
     9181 gatactttta actcatggga ctaccgtcag gttgagtatt attaggatag agaagaagtc
     9241 tagtgttatg agatattaaa gctcctaaca ctgggctttt gtttagaatt tcttttcttt
     9301 ttctatagga tatggtattc tatgtagaaa atatatgtta ataagtaatg ccaatattta
     9361 aacatcatta gtaaaaggag ttagattgat gaataaaaga aaagttagtt tagaagattt
     9421 ttataaatgg tatagtctaa ataaagaaga gttattaaat aaggcaactg ttggtgaaaa
     9481 gtttaatgat aaattaaaag aagagtttct ccaggaatgg cctttggata ggattttaac
     9541 aatgtcaatc gatgaatatg taataggaaa gggacagcaa aataagtctt tatgctacgc
     9601 tcttgagaag ggaaaataca aaaatctatt tcttggaatt tctggtggct cagcttcaaa
     9661 atttggtatt tattggaata aaaaaacaaa caaatataaa gatcaagcta ataatgagat
     9721 ttcagagttg gatcagcgat tttcaaaatt aaaatcagat ttgtatgaaa ttatcaaaga
     9781 aggtattcgt tttaactttg aaaatcctat ttttgatatg aaaagatcaa caaatgaatt
     9841 tattggtcgt tctgctatgg tgacaaaatt actttgtctc tatactgagg gagatccttt
     9901 ctttggtgta aatattaata gtcagaaaga attttggaac cactttgttt ctcagacaaa
     9961 tcaaggtgga ccttatctgc aaaatcataa aataattgaa ctggtgtcca aaacttatcc
    10021 tgagttggag ccatcgaaat taggaactat gctttttgag tattctaagc tttttatgga
    10081 aaataaggaa gacaatagta caatggattc atcaaacaat tttcgtcatc aattaactca
    10141 atctctatta aagtctccaa acctcatcct ccgcggtgct cctggcacgg gaaaaactta
    10201 tcttgctaaa gaaattgcta aagaattaac ggatggcaac gaagatcaaa tcggatttgt
    10261 acaatttcac ccatcatatg attatacgga ttttgtagaa ggtttaagac cagtatcaaa
    10321 tggggatgga gctattgagt ttaggctaca ggacggtatt tttaaagatt tttgtcagaa
    10381 agcaaaagaa acccaattga ttggaggaca agataatttt gatgaggctt gggattctta
    10441 cttagaatat ataaatgttg ctgaagaaaa agaatatata acaaaaacat cttacttatc
    10501 tgttaatagt agacaaaatt tgtcagtaaa ttatgatagt ggtgttccag gatggtcact
    10561 acctagcaaa tatgtttacg agttgtataa agataaaaat tataataagc aagaatacta
    10621 caaaagtggt ggaaaaactg tcctagaaac attgagaaag agatttggtt tgaaagacta
    10681 tgtttcccca acagaaattg atactgataa gaaattcgtc tttatcatcg atgaaatcaa
    10741 tcgtggggag atttctaaga tttttggcga actctttttc tctatcgacc ctggctatcg
    10801 tggtgaaaaa ggaagtgttt ctacccaata tgcaaatcta cacgaaactg atgaaaagtt
    10861 ctatatcccc gaaaatgttt acatcatcgg aactatgaat gatattgatc gttcagtgga
    10921 tacctttgat tttgctatgc gtcgtcgttt tcgttttgtt gaagttactg ccgagggtca
    10981 agttggcatg ttggataaag agctgaatat ccatgcagaa gaagcaaaaa ttcgtctaag
    11041 aaacttgaac gctgctatcg aaaatgttca ggaattaaac agtcattatc atattggacc
    11101 aagttatttt cttaagttga aggatgtaga ttttgactat gaattactct ggtccgatta
    11161 tattaagcct ctcctagaag actacttgcg aggttcttat gatgaggttg aaactttgga
    11221 aactttgaaa aaagcatttg aactgacaaa taatgagcaa aaagatcagg cagtagctga
    11281 tgacaatgaa ggtgatgaaa acgatgatgc agattactga taatcaacac aagattatta
    11341 aagaaaaatt tgttgaagaa tatcctaaac taagcaatct tcttttagac agaaccttgg
    11401 aaagtctatc ccaagatgaa cgtattttca tttttccaaa tgatttgact catactcctg
    11461 atttggataa ggaccaaaag atttttgaaa cagtcaatca gaaaatcaag acagggaacg
    11521 tgattggttt tcttggatat ggtcaggaaa gattaacgat ttcctcacga ttttctgatg
    11581 agagtaatga ccactttttg cattatctct taaacaaggt tcttcatatc aatctcacta
    11641 gtttagatgt tgctttgtct cgtgaagaga ggctttatca acttttggtg tatctctttc
    11701 ccaagtatct acaagctgct attcgaaaag gtctttataa ggaatatcat cgattttctc
    11761 ataacgacag tcatgttaag ggagtgattg atgtaagaaa ccatctcaag aaaaatcttc
    11821 ctttcacggg aaatattgcc tacgcaacga gagagttcac ctatgataat cccctcatgc
    11881 agttggtccg tcacactatt gaatacatta agaatcagaa aagcattggt caaggggtac
    11941 tagataatct ctcaactagt cgtgaaaacg tatctgaaat cgtgcgtgta acgccctctt
    12001 ataaactagc tgatcgtgct aagattattc ggggaaatca atctaaacct atacgtcatg
    12061 catactttca cgagtacaga aacttacaag aactttgtct gatgatccta aaccaagaaa
    12121 agcacggttt agggtatcaa gatcaaaaaa tctatggtat tctctttgat gttgcctggc
    12181 tttgggaaga gtatgtttac accttgttgc caaaaggttt tgtacatccc agaaataagg
    12241 ataagacgga tggaatttca gtattttctg ttgggaaacg aaaagtatat ccagattttt
    12301 atgacagaga acgaaagatt gttctagatg caaaatataa aaaactggaa ttgactgaaa
    12361 aaggaatcaa ccgtgaggac ttattccagc tgatttccta ttcttatatt ttaaaagctg
    12421 agaaggctgg actgattttt cctagtatgg agcagtcagt aaatagtgaa ataggaaaag
    12481 tagctggcta tggagctcaa ttgaagaagt ggtctattcg aatccctcag aatgcctcat
    12541 tctatagtac attttgtaaa atgatggaaa attcagaaga aaattttaaa gcgattattg
    12601 atgaagaagt ggggagaaaa taatttcccc tctttctttt atttttaaca caagtaaaac
    12661 ccgaacgata tattttaatt cataaaataa tttgacaaaa actgtacttt gatttataat
    12721 aaattaagga aacgtcggaa aaggcgtagt gatgcaagag cataggtagg tcattacaaa
    12781 agaaacgaga catcgatatg ttaaatgaat ttccaatttt tgattacgaa gatattcaat
    12841 tgattccaaa taaatgtgtc attaaaagcc gtgcagaagc ggatacaagt gtcactttag
    12901 gaaatcacac ctttaaacta cctgttgtgc cagcgaatat gcagacgatt ttggatgaaa
    12961 atgtagcaga gcaactggct aaaggtggtt acttctacat tatgcaccgt tttgatgagg
    13021 caggacgcat tccttttatt aaacgaatgc acaatcaagg gctcattgct tctatctctg
    13081 tcggtgttaa ggattatgag tatgattttg ttagccagct caaggctgat actcctgagt
    13141 atatcactat tgatattgct catggtcatg cggatagcgt gatttctatg attcaacaca
    13201 tcaagaaaga attgccagat acctttgtca ttgctggaaa tgtgggaaca ccagaagctg
    13261 tgcgtgaatt ggaaaatgct ggtgcggatg ctactaaggt cggaatcggt cctggtaagg
    13321 tttgtatcac caaggttaag actggttttg gtacaggtgg ttggcagttg gctgctctac
    13381 gctggtgtgc caaggctgcg cgtaaaccga ttatcgctga tggaggaatt cgtactcacg
    13441 gtgatattgc taagtctatc cgcttcggtg ctagcatgat catgattggt tccctctttg
    13501 caggacacat tgaaagtcca gggaaaacga ttgaagtcga tggtgaacag ttcaaagaat
    13561 attatggttc agcctcacaa tatcaaaaag gtgcttacaa aaatgtggaa ggcaaacgta
    13621 tcttgcttcc tgctaaaggt catctgcaag acactttaac tgagatggaa caagaccttc
    13681 aaagtgctat ttcttatgca ggtggacgtc aggttgctga ccttaaacac gttgattatg
    13741 tgatcgtgaa aaactccatc tggaatgggg atgcttccca ctaagacggg gtctttgacc
    13801 aaaaaacttg ttattagagc aaatttctgt tataataaaa caagtttcca cccttagtgt
    13861 aatggatatc acgtaagatt ccggttcttg agatgggggt tcgattccct cagggtggat
    13921 gtaaacatcc taaaaagcct ttaatagggc ttttttcttt atcctgaccc aaatttgccg
    13981 ctaaaactga gaatctctgt ctaaagaatt cccaaatgct ttccagtccc gttttagagt
    14041 gactcagggg gctttttttg atataataaa aaggactatt atcagttaga aagaggttgg
    14101 tatgaaagaa ttacaaactg tactaaagaa tcattttgca atcgaatttg cagacaaaaa
    14161 tttactggaa actgccttta ctcatacgag ttatgccaat gagcaccgcc tcttaaaaat
    14221 ttcacacaat gaacgcttgg aatttttagg agacgctgtt ctacagttat tgatttcaga
    14281 atatctatat aaaaaatatc ctaaaaagcc tgaaggtgac ctatcaaaac tccgtgctat
    14341 gattgtccgt gaggagagtt tagctggttt tgcgcgtgat tgccagtttg accagtttat
    14401 caagctgggt aagggggaag aaaaatctgg tgggcgcaat cgtgatacca ttcttggtga
    14461 tgcctttgaa gcctttcttg gtgcccttct tttggacaag gatgtggcca aggtcaagga
    14521 atttatctat caagtcatga ttcctaaggt tgaagcaggc gaatttgaga tgattacaga
    14581 ctataaaacc catctccaag agttgcttca ggtcaatggt gatgtggcta ttcgttatca
    14641 ggtgatttct gaaacgggtc ctgctcacga taaggtcttt gatgtagaag ttcttgttga
    14701 aggtaagagc atcggtcaag gtcaaggtcg ttctaagaaa ttagcagagc aggaagctgc
    14761 caaaaatgcc gttgagaaag ggctggattc atgtatttaa aggaaatcga aattcaggga
    14821 ttcaagtctt ttgctgataa gaccaaggtc gtctttgacc aaggtgtgac ggcagtcgtt
    14881 ggacccaatg gatctggaaa gtcgaatatt acagaaagtc tgcgatgggc cttgggagag
    14941 tctagtgtca agagtctccg tgggggcaag atgccggatg tcatctttgc tggaacagaa
    15001 agtcgtaaac cgcttaatta tgcttctgta gttgtgactc tggataatca tgatggattt
    15061 atcaaggatg caggtcaaga aatcagagta gaacgccata tctatcgtag tggggatagt
    15121 gaatacaaaa ttgacggcaa gaaagttcgt cttcgtgata ttcatgatct cttcttggac
    15181 acaggtttgg gacgagattc cttttccatc atttcccaag gcaaggttga ggagattttt
    15241 aattctaagc ctgaggaacg ccgagctatt tttgaagaag ctgcaggagt tttaaaatac
    15301 aagactcgta gaaaagaaac tgagagcaaa ttgcaacaaa ctcaggataa tctggaccgc
    15361 ttagaggaca ttatctacga gttggataat caaatcaagc ctcttgagaa gcaagctgag
    15421 aatgcccgta agtttttaga cttggaagga caacgtaagg ctatttattt agacgtgctg
    15481 gttgctcaaa tcaaggataa taaggctgaa ctagactcga cagaagaaga gttggctcag
    15541 gttcaggaac tcttgactag ttattaccaa aagcgtgaaa aattagaaga ggaaaatcaa
    15601 actcttaaaa agcaacgcca agatttgcag gctgaaatgg ccaaagacca aggcagtttg
    15661 atggatttga ccagtctgat tagtgactta gagagaaaat tagccctatc gaaactggaa
    15721 tctgagcaag tagccctcaa tcaacaggaa gcacaagccc gtttggctac tttggaggat
    15781 aagagaagtt cactcagcca agaaaaatct gataaagaaa gctctttagc tctgttagag
    15841 ggaaatctag tccaaaataa tcaaaaactc aatcgtttag aagctgaatt gctggctttc
    15901 tcagacgatc ctgatcagat gattgagctc ttacgtgaac gctttgtagc tcttttacaa
    15961 gaagaagcgg atgtctcaaa ccagttgacc cgtattgaga atgagttgga aaatagtcgt
    16021 cagctttctc aaaaacaagc agatcaacta gaaaagctga aagagcaatt ggctatagct
    16081 aaagagaagg ctagtcagca aaaggaagag cttgaaactg ccaaggagca ggttcagaaa
    16141 ttattggctg actaccaagc tattgccaag gagcaagagg agcagaaaac ttcctatcaa
    16201 gctcaacaaa gccaactctt tgaccgtctg gataatctca aaaacaagca ggccagagct
    16261 caaagtttgg aaaatatcct gagaaatcat agtaactttt atgcaggtgt taagagtgtt
    16321 ctccaagaaa aagatcgcct aggtgggatt attggtgcag tcagtgagca tctgaccttt
    16381 gatatgcatt atcaaactgc cctagaaatt gcacttggag cgagtagtca gcacattatt
    16441 gtagaagatg agaatgcggc aaccaaagcc attgatttcc tcaaacgaaa cagagccggt
    16501 cgtgcaacct ttcttccttt gaccactatt aaggcgcgta cgatttctag tcagaaccaa
    16561 gatgctatcg ctgtaagccc aggtttcctt gggatggcag atgagttagt tactttcgat
    16621 actagactgg aagccatttt caagaacttg ctagctacga cggctatctt tgataccgta
    16681 gaacatgcgc gtgaagctgc tcgacaagtt cgttatcagg ttcgcatggt gacattggat
    16741 gggacagagc ttcgtacagg tggttcctat gcaggtggag ccaatcgtca gaacaacagc
    16801 atcttcatca agccagaact ggagcaatta caaaaagaaa ttgctgaaga agaagcaagc
    16861 ttggattcag aagaagtggc tttgaagacc ttgcaagatg agatggccag attgaccgag
    16921 tcattagaag ctattaaatc tcaaggagag caggcacgta ttcaggagca aggcttgttc
    16981 ctcgcttatc agcaaactag tcagcaagtt gaagagctag aaactctttg gaaactccaa
    17041 gaagaggaaa tagatcgtct ttccgaggga gattggcaag cggataagga aaactgccaa
    17101 gagcgtcttg ctgcaatcgc cagtgacaag caaaatctgg aagctgagat tgaagagatt
    17161 aagtctaaca aaaatgccat ccaagaacgc tatcaaaact tgcaggaaga gctagcgcaa
    17221 gctcgtttgc ttaagacaga actgcaaggg caaaaacgtt atgaagttgc tgatattgag
    17281 cgtttaggca aggaattgga taatcttaat atcgaacaag aagaaatcca gcgcatgctc
    17341 caagaaaaag ttgacaatct tgagaaagtt gatacggatt tgctcaatca gcaggccgaa
    17401 gaagccaaaa ctcagaaaac aaatctccaa caaggtttga ttcgcaagca gtttgagttg
    17461 gatgatattg aagggcagct ggatgatatt gctagtcatt tggatcaggc tcgccagcag
    17521 aatgaggagt ggattcgcaa gcaaacacgt gctgaagcta agaaagaaaa ggtcagcgaa
    17581 cgcttgcgcc atctacaaag tcaattaaca gaccagtacc agattagcta tactgaagct
    17641 ctagaaaaag cgcatgaatt ggaaaacctc aatctggcag agcaagaagt taaggattta
    17701 gagaaggcta ttcgctcact gggtcctgtc aatatagaag ctattgacca gtacgaagaa
    17761 gtttacaacc gtctggactt tctaaatagt cagcgagatg atattttgtc agcgaaaaat
    17821 ctgctccttg aaaccattac agagatgaat gatgaggtta aggaacgctt taaatcaacc
    17881 tttgaagcta ttcgtgagtc ctttaaagtg acctttaagc agatgtttgg cggaggtcag
    17941 gcagacttga tattgactga gggcgacctt ttaacagctg gtgtggagat ttctgttcaa
    18001 cctccaggta agaaaatcca gtcgcttaac ctcatgagtg gtggtgaaaa agctctatcg
    18061 gctcttgcct tgctcttctc cattattcgt gtcaagacta ttccatttgt tatcttggat
    18121 gaggtggaag ctgcgctgga tgaagctaat gttaaacgtt ttggggatta cctcaaccgc
    18181 tttgacaagg acagccagtt catcgtcgta acccaccgta agggaaccat ggcagcggct
    18241 gattctatct atggagtgac catgcaagaa tcaggtgtct caaaaattgt ttcagttaag
    18301 ttaaaagatt tagaaagtat tgaaggatga caattaaact agtagcaacg gatatggacg
    18361 gaaccttcct agatgggaat ggacgctttg atatggatcg tctcaagtct ctcttggctt
    18421 cctacaagga aaaagggatt tactttgcgg tggcttcggg tcggggattt ctgtctctgg
    18481 aaaaattatt tgctgatgtt cgtgatgaca ttattttcat cgcggaaaat ggcagtttgg
    18541 tagagtatca aggtcaggac ttgtatgaag cgactatgtc tcgtgacttt tatctggcaa
    18601 cttttgaaaa gctgaaaacg tcaccttatg tagatatcaa taaactgctc ttgacgggta
    18661 ataagggttc atatgttcta gatacggttg atgagaccta tttgaaagtg agtcagcatt
    18721 ataatgaaaa tatccaaaaa gtagcgagtt tggaagatat cacagatgac attttcaaat
    18781 ttacaaccaa cttcacagaa gaaacgctgg aagatgggga ggcttgggta aacgaaaacg
    18841 ttcctggtgt taaggctatg acaactggct ttgaatctat tgatattgtt ctggactatg
    18901 tcgataaggg agtggccatt gttgaattag ttaaaaaact tggtatcaca atggatcagg
    18961 tcatggcttt tggagacaat cttaatgact tacatatgat gcaggttgtg ggacatcctg
    19021 tagctcctga aaatgcacga cctgaaattt tagaattagc aaagactgtg attggtcacc
    19081 ataaggacca gtcggttata gcttatatgg agggcttata atggcagata taaaattaat
    19141 cgcattggac ttggacggga ccttgctgac tactgataaa aggctgacgg atcgtaccaa
    19201 ggaaaccttg aaagctgcgc gtgatcgtgg tatcaaggtc gtattgacaa ctggtcgtcc
    19261 cttaaaagct atggatttct ttctccatga gctagggact gacggtcagg aagatgagta
    19321 taccattact tttaatggtg gtttggttca gaaaaataca ggtgaaatcc ttgataagac
    19381 agtcttttca tatgatgatg tggcacgctt gtatgaagaa acagagaaat tatcactgcc
    19441 tcttgacgct atctcagaag gaactgttta tcaaattcaa tcggaccaag aaagtcttta
    19501 tgccaaattc aatccagctt tgacttttgt tccagttgat tttgaagacc tgtctaatca
    19561 aatgacctac aataaatgcg tgaccgcctt tgctcaagaa cccttggatg cagccattca
    19621 gaagatttct ccagaattgt ttgaccaata tgaaatcttt aaatcacgtg aaatgttgct
    19681 agaatggtca ccaaagaatg ttcataaagc aacaggtttg gcaaaactaa ttagccatct
    19741 tggaattgat caaagtcaag tgatggcctg tggtgacgag gccaatgacc tctctatgat
    19801 tgaatgggca gggctcggtg ttgccatgca aaacgctgtt cctgaagtca aggcagtcgc
    19861 aaatgtggta actcctatga ccaatgatga agaagctgtc gcctgggcta tcgaagaata
    19921 tgtgctaaag gagaactaag atatgggatt gtttgaccgt ctattcggaa aaaaagaaga
    19981 acctaaaatc gaagaagttg taaaagaagc tctggaaaat cttgatttgt ctgaagatgt
    20041 tgatcctacc ttcacagaag ttgaggaagt ttctcaggaa gaagcagagg ttgaaattgt
    20101 tgaacaagct gtgttccaag aagaggaaat ccaagacaca gttgaagaaa gtctggattt
    20161 agagccagtt gtagaagttt ctcaaaaaga agtcgaagaa tttccacact cagaagaagg
    20221 gaatactgag tttctagaga ctgtagaaga aaataattct gaaattcttg aagcagaaag
    20281 gcctcaaaca gaagaaactg ttcaggaaaa atatgaccgc agtcttaaga aaactcgcac
    20341 aggtttcggt gcccgcttga atgccttctt tgctaacttc cgctctgttg acgaagaatt
    20401 tttcgaggaa ctggaagaac tgttgattat gagtgatgtt ggtgtccaag tcgcttctaa
    20461 cttaacggag gaactacgtt acgaagccaa gcttgaaaat gccaagaaac ctgatgcact
    20521 tcgtcgtgtc atcattgaga aattggttga gctttataaa aaggatggta gctacgatga
    20581 aagcatccac ttccaagata acttgacagt tatgctcttt gttggtgtga atggtgttgg
    20641 gaaaacaact tctatcggaa aactagccca ccgctacaaa caagctggtg agaaggtcat
    20701 gctggttgca gcagatacct tccgtgcggg tgcagtagct cagctagctg aatggggccg
    20761 acgagtagat gttccagtag taactggacc tgaaaaagct gatccagcca gcgtggtctt
    20821 tgatggtatg gaacgtgccg tggctgaagg tatcgatatt ctcatgattg atactgctgg
    20881 tcgtctgcaa aataaggata accttatggc tgagttggaa aagattggtc gtattatcaa
    20941 acgtgttgtg ccagaagcac cacatgaaac cttcttggca cttgatgcat caacaggtca
    21001 aaatgcccta gtacaggcca aagaattttc gaaaatcaca cctttaaccg gaattgtttt
    21061 gactaagatt gatggaactg ctcgaggagg tgtggttcta gccattcgtg aagaactcaa
    21121 tattcctgta aaattgattg gttttggtga aaaaatcgat gatattggag agtttaactc
    21181 agaaaacttc atgaaaggtc tcttggaagg tttaatctaa tcagaagcaa aaatcctggg
    21241 ctctttgtca actgtagtgg gttgaagtca gctaagcttg agaaaggaca aatttcgtcc
    21301 tttctttttt gatgttcaaa gcgataaaaa tccgtttttt gaagttttca aagtttcgaa
    21361 aaccaaaggc attgcgcttg ataagtttga tgagattatt ggtcgcttcc agtttggcat
    21421 tagaatagtg tagttgaagg gcgttgataa tcttttcttt atctttgagg aaggttttaa
    21481 agacagtctg aaaaatagga tgagcctgct taagattgtc ctcaataagt ccgaaaaatt
    21541 tctctggttc cttattctgg aagtgaaaca gcaagagctg atagagctga tagtggtgtt
    21601 tcaagtcttc tgaatagctc aaaagcttgt ctaaaatctc tttattggtt aaatgcatac
    21661 gaaaagtagg acgataaaat cgcttatcac tcagtttacg gctatcctgt tgtatgagct
    21721 tccagtagcg cttgatagcc ttgtattcat gggattttcg atgaaactga ttcatgattt
    21781 gaacacgcac acgactcata gcacggctaa gatgttgcac aatgtgaaag cgatcaagaa
    21841 cgattttagc attcgggagt gaaacagtct gggagactgt ttcagcctga gcctagaaat
    21901 ttgaaagcga agctgtttag ccaagtcata gtaagggcta aacatatcca tagtaataat
    21961 tttgacgcga catcggacag ctctatcgta gcgaagaaag tgatttcgaa tgatagcttg
    22021 tgttcttccc tcaagaacag tgatgatatt gagattgtta aaatcttgcg caatgaagct
    22081 catctccatc tcccgattga aacagtcact ccccggactg tttcaacgtc ccaggacata
    22141 atctcaggaa gacgcgaaaa atcatgctca aagtgagaat cattgagctt gcgaatgaca
    22201 gttgaagttg agatggaaag ctgatgggca atatcagtca tagaaatctt ttcaattaac
    22261 ttttgagcaa ttttttggtt gatgatacga gggatttggt gatttttctt taccagggga
    22321 gtctcagcaa ccatcatttt tgaacagtga tagcacttga aacggcg
//
LOCUS       DAVKHZ010000028        21589 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000028 DAVKHZ010000000
VERSION     DAVKHZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.028"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(145..2034)
                     /locus_tag="VWW99_001001"
     CDS             complement(145..2034)
                     /locus_tag="VWW99_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9538858.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2172..3737
                     /locus_tag="VWW99_001002"
     CDS             2172..3737
                     /locus_tag="VWW99_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9538859.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3737..4477
                     /locus_tag="VWW99_001003"
     CDS             3737..4477
                     /locus_tag="VWW99_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9538860.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4586..4801)
                     /locus_tag="VWW99_001004"
     CDS             complement(4586..4801)
                     /locus_tag="VWW99_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538861.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5672..6922)
                     /gene="ilvA"
                     /locus_tag="VWW99_001005"
     CDS             complement(5672..6922)
                     /gene="ilvA"
                     /locus_tag="VWW99_001005"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9538862.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6972..7328)
                     /locus_tag="VWW99_001006"
     CDS             complement(6972..7328)
                     /locus_tag="VWW99_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9538863.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7566..7802)
                     /locus_tag="VWW99_001007"
     CDS             complement(7566..7802)
                     /locus_tag="VWW99_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538864.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7909..8931)
                     /gene="ilvC"
                     /locus_tag="VWW99_001008"
     CDS             complement(7909..8931)
                     /gene="ilvC"
                     /locus_tag="VWW99_001008"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9538865.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8997..9473)
                     /gene="ilvN"
                     /locus_tag="VWW99_001009"
     CDS             complement(8997..9473)
                     /gene="ilvN"
                     /locus_tag="VWW99_001009"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9538866.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQFNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9466..11166)
                     /locus_tag="VWW99_001010"
     CDS             complement(9466..11166)
                     /locus_tag="VWW99_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9538867.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVKFHA
                     "
     gene            complement(11366..13033)
                     /locus_tag="VWW99_001011"
     CDS             complement(11366..13033)
                     /locus_tag="VWW99_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9538868.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13036..13401)
                     /locus_tag="VWW99_001012"
     CDS             complement(13036..13401)
                     /locus_tag="VWW99_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9538869.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13558..13746)
                     /locus_tag="VWW99_001013"
     CDS             complement(13558..13746)
                     /locus_tag="VWW99_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9538870.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13862..14038)
                     /locus_tag="VWW99_001014"
     CDS             complement(13862..14038)
                     /locus_tag="VWW99_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9538871.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14149..14346)
                     /locus_tag="VWW99_001015"
     CDS             complement(14149..14346)
                     /locus_tag="VWW99_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9538872.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(14601..16145)
                     /locus_tag="VWW99_001016"
     CDS             complement(14601..16145)
                     /locus_tag="VWW99_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9538873.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16358..16660
                     /gene="gatC"
                     /locus_tag="VWW99_001017"
     CDS             16358..16660
                     /gene="gatC"
                     /locus_tag="VWW99_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9538874.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTNRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16660..18126
                     /gene="gatA"
                     /locus_tag="VWW99_001018"
     CDS             16660..18126
                     /gene="gatA"
                     /locus_tag="VWW99_001018"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9538875.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPATFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18126..19568
                     /gene="gatB"
                     /locus_tag="VWW99_001019"
     CDS             18126..19568
                     /gene="gatB"
                     /locus_tag="VWW99_001019"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9538876.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKETKGQANPQVALKLLAQELAKLKEN"
     gene            19697..20257
                     /gene="efp"
                     /locus_tag="VWW99_001020"
     CDS             19697..20257
                     /gene="efp"
                     /locus_tag="VWW99_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9538877.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20279..20668
                     /locus_tag="VWW99_001021"
     CDS             20279..20668
                     /locus_tag="VWW99_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9538878.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20661..21083
                     /gene="nusB"
                     /locus_tag="VWW99_001022"
     CDS             20661..21083
                     /gene="nusB"
                     /locus_tag="VWW99_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9538879.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAHTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            21215..>21589
                     /locus_tag="VWW99_001023"
     CDS             21215..>21589
                     /locus_tag="VWW99_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9538880.1"
                     /translation="MEQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAH"
BASE COUNT         6419 a         4938 c         3899 g         6333 t
ORIGIN      
        1 ggctttttgc cgtgctctaa aatcatcgcc aaatcatcaa attgaaataa gaaatagttt
       61 agataaaact attgactttt cttatagttt gtctttcttc tatcttccat tatatcatac
      121 tttttcagtc tatggtagct ctctttaaaa ggctccgata ctgccgccac ctccgcctcc
      181 agagaagcca ccaccagaac ttccacttcc agaagataca gagtaagtac ttgctgtatt
      241 tgcgacacta gcataatggc tcatttgcgc gcttgaatga taaaacatac tgtgccagcc
      301 ataagctaca tagagattga tatctggatt ttcaacttga atctgatgaa ccttcatcaa
      361 atgactaacc ttgtccgcat agccaaataa ggttgcatag accaagagac gattccaaac
      421 aacaatactt tccaattcag ccttatccag tcgtgcaatc tcacgtaaca tattttcaaa
      481 actggtccag agatagtaga cctcagctcc cgcttcattt agaacaccat cacgattatc
      541 tagtcgaagc ttccaataat agaaaacaga caaaactaac cctagaaaac caagtattgg
      601 caaagggagg taaagatagc catgaacgtc taaactgtac aagaacaaac caaatccgat
      661 aaataggggc aagatagtca agacacccat acccacttgc aaagcctttt ccaaaccagt
      721 taaaggacga tagtaatctg ggagtcccca gaagacaact cgctttctca ctccttcttg
      781 catctctttt aatactgctt cgaaagaaga tttgagctga atccctcttg cttgaatccg
      841 tttctcatca gcagctttag ctccgcgata aaggctgtca gataccttgt aatccgcaaa
      901 caaattggaa agagtggctt cttttttgcc tgaaaaggct agatttagac agtctctctc
      961 aaatcttgac aaaccatctt ctttcaccaa tctcaagcca actgcatctc cttctgaaat
     1021 aatagaaaca ttcccacggt ccatcacatc tagcaaggta gcttgaataa gttggtcaaa
     1081 agtgaatttg ccagccccct tgactaaggg actcacttcc tccaaggagg tcgagtagac
     1141 agcctctgat aaaaccatag gctctaattc cattggtggt tcatagagac gatgattttt
     1201 ggcatatttg actgaaggag tggtctttct tctataaata aaatagaagc agacactcaa
     1261 taacaaggag atagaaagga tcgaagggag gacccaagta acaagttgtt tactctgatc
     1321 tttttctcta acaatcgagt cttctatctt attaaactct tctaaacgat tccctttcaa
     1381 tccctgatcc ctagcgctag caaaatcggt ccgaggccag taggcatgca actcaactcc
     1441 acgcttagcc ggaagattgt ctaaacggat agtataatca aggttactct tttcaatcgt
     1501 tccctctcta aaaagtttcc ctgtatggaa aaagagtttt tcagccccct tgtctcccct
     1561 tacatgaaat tcaaactttt caatagactc tgaactatct gtcagaggtt gccaatttaa
     1621 ttcagcgata tcatcataaa ggaaaagtaa attttttaag ttccagacga ggtcaacttc
     1681 aactatgtcg ccctcctgac ctggattata gactctcaca gtataaccat ccgcttcttc
     1741 tgttacttcg ctagtcacat ctgctagttc tgcaccgttt ttcgcggcct gaacctttgg
     1801 atgagggtca atgtcaaacc cgctaggcat cttaccagca cgtccaagtc ccacgatttg
     1861 tcccttaaag tcctcctcaa actggtaaac tatcttctgt ctaaactctg ccgtattatc
     1921 tgcatgaata tacaaatccc cttgataaga gtttatcttg aaatcaatgg caaaaacaga
     1981 gagtggaaga aggcaaaaca agcctaacac cagtaagaaa aaagtttttt tcatcaacta
     2041 agcccccttt ttatctttct tatcattata gcattttttc gtaagtaaag gcttacttat
     2101 gttgaaaaat tacgcatttt tcgataaaat aggagacagt tattttttaa tagatcagaa
     2161 taggagaaat catgagaaaa atatacttat ctattttcac aagtctcttg ctgatgctag
     2221 gacttgtcaa tgttgctcaa gccgatgaat atttacgcat cgggatggaa gcagcatatg
     2281 ctccctttaa ctggacccag gatgatgata gcaacggagc tgtcaaaatc gatgggacca
     2341 atcagtatgc caacggatac gatgttcaaa tcgccaagaa aatcgctaag gacttaggta
     2401 aagaaccttt ggttgttaaa accaagtggg aaggtctagt ccctgccctt acttctggta
     2461 agattgacat gattatcgca ggtatgagtc caactgcaga acgcaaacaa gaaattgcct
     2521 tttcgagcag ttactacact agcgaaccag ttttgcttgt caaaaaagat tctgcctacg
     2581 caagtgctaa atctttggat gactttaacg gtgcaaaaat cacttctcaa caaggggtct
     2641 acctctacga cttgattgca caaatcccag gtgctaaaaa agaaacagcc atgggagact
     2701 tcgctcaaat gcgccaagct cttgaggctg gtgtcattga tgcttatgtt tctgaacgtc
     2761 cagaagcact gactgctgaa gctgcgaact ctaagttcaa gatgattcaa gtagaacctg
     2821 gtttcaaaac tggggaagaa gatacagcta tcgctatcgg gcttcgtaaa aatgacaatc
     2881 gtattagcca aatcaatgcc agcattgaaa ccatttcaaa agatgaccaa gttgccttga
     2941 tggatcgtat gatcaaggaa caacctgccg aagctacaac aactgaagag actagcagta
     3001 gtttctttag ccaagtcgct aaaattcttt ctgaaaactg gcaacaactc ttgcgtggtg
     3061 ctggtatcac tcttttaatc tctatcgtcg gaaccatcat aggtctcatt attggacttg
     3121 ccattggtgt cttccgtact gctcctctct ctgaaaacaa agccatttat ggccttcaaa
     3181 aactagtcgg ctgggttctc aatgtctaca ttgaaatttt ccgtggtacg ccaatgattg
     3241 ttcaatcgat ggttatctac tatggaactg cccaagcttt cgggatcaac cttgaccgta
     3301 cactagctgc tatcttcatc gtttcaatca ataccggtgc ctacatgact gaaatcgtcc
     3361 gtggtggtat cctagcagtt gacaagggac aatttgaagc tgcgactgct cttggtatga
     3421 cccataacca gaccatgcgt aagattgtcc tacctcaggt agtccgtaac atcctacctg
     3481 caactggtaa tgaatttgtc atcaatatca aagatacatc tgtattgaac gttatctctg
     3541 ttgtcgaact ttatttctca ggaaataccg tggcaacaca aacctatcaa tacttccaga
     3601 catttacaat catcgccgtg atttactttg tcctcacctt caccgtaaca cgtatcctac
     3661 gctttatcga gcgcagaatg gacatggata cctacactac aggtgctaac caaatgcaaa
     3721 cggaggattt gaaataatga cacaagcaat ccttgaaatt aaacacctca aaaaatccta
     3781 tggacaaaac gaagtgctaa aagacatttc actcactgtc cacaagggag aggtcatctc
     3841 tatcatcgga agctctggaa gcggaaaatc gaccttccta cgctccatta acctacttga
     3901 aacaccaact gatggacaaa tcctttatca tggacaaaac gtcctcgaaa aaggctatga
     3961 cctcacgcaa taccgtgaaa agttggggat ggttttccaa tcctttaacc tctttgaaaa
     4021 tctcaatgtt cttgaaaaca caatcgtcgc tcagacaact gtcctaaaac gcgaacgcac
     4081 agaagctgaa aagattgcca aagaaaacct ggaaaaggtc ggcatgggag aacgctactg
     4141 gcaagccaaa ccaaaacaac tctcaggtgg tcaaaaacaa cgtgtggcca tcgctcgtgc
     4201 cctctccatg aatccggacg ccattctctt tgatgaacca acatcagctc tcgatccaga
     4261 aatggttgga gaagtcctca aaatcatgca ggacctggct caggaaggct tgaccatgat
     4321 tgtcgtaacc catgaaatgg aatttgcccg tgatgtctct caccgtgtta tctttatgga
     4381 taagggcgtg atcgctgaag aaggtaaacc agaagacctc ttcaccaatc ctaaagaaga
     4441 ccgaacaaaa gagttccttc aacgctatct caaataaaaa gaaaaggctg catcaaccgt
     4501 gcagtctttt tgctgtcctc aaaatgaaaa ggcagattct ctatctctac tacttgaagg
     4561 tcaagaatcc gccgttatcc aaagattaat cttcaaattt ttcataattt ttttcataga
     4621 aatcaattag gcctagggct gtttcaaatg aaatattttt tacttttgca cgaccctgcg
     4681 ccagagcaat gatagacatt tcacgcgcat tcgtttcttt agagatacgg tagcctgtga
     4741 ttttcttatc acgaacccag ccaacaactg attctacttt ttcaaagttt gacttagcca
     4801 tgtttttctc ctattttctt ctttataata tttaattgta tacgttttta ctacttttgt
     4861 caataaaaaa aacatatatt caacgaaaat aattagttca gaattttctt acttcctttt
     4921 taaatccgat aatatatagg ttttgctagc ctctacacca taaatatttg ctaataaaac
     4981 tactgtatta ttaccctctt aaggtgcgaa attattcaag ttcttaaaaa atgcttctct
     5041 atttccgtaa gatagcttat ttcttcgatt taagaactga agatgatagt tctaaccttt
     5101 agaaatccaa gccttcacta gctcaaaaac tcctatcttt cttcctcaat aaatcatctg
     5161 agagataggt attccttata tccttcaaat tcatcactcc tttggatatt gaggtgctag
     5221 cctagaagca gacacccgat tatctaaacc atccttaata gagatgacaa gacagataag
     5281 ctcagttaat tacttatata tctttttaat atggtatatt tgtcaacaaa aagaaatcaa
     5341 atcatacctt atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaagttgct
     5401 caaaacactg ttttgaggtt gcagatagag ctaacgtggt ttgaagagat tttcgaagag
     5461 tattaaagac agtgtgacta tgcttgtcaa tagataggaa atcaaaatga aggatggaaa
     5521 tctgagccaa acatcataag tgctataatg aacagaagta agaattgcac gataagtttt
     5581 ctaaaaccat aggaatcgtc ccctttcttc atcttcatta tagcacatat acatcagttg
     5641 tacaaataat atgatatttt tttattagtc ctcagacaag catattataa agcgtttcat
     5701 taccatttaa gttaatataa gctggatcaa aaccttccat tctacgaatc aaacctgcat
     5761 aatcatgctt atctgctaaa gcgatcccaa ttaatactgg gcctgttccc ttgctagctc
     5821 gtttgatata ctcaaaacgt gtgatatcat catttggccc caggatatca tttacaaact
     5881 cacgcaaagc tcctggacgt tgtgggaaat tgaccacaaa gtaatgtttg ataccatcat
     5941 aaatcaaggc acgctcttcc atttctggca tacggttgat atcattattt cctccagaaa
     6001 tgatacaaca aatggttttc cccttaatat attcagctaa aacctctaaa gaggcgatac
     6061 tagctgctcc agcaggttct gcgactatcc cttgcttaga gtaaaggtca atcaaggttt
     6121 cagaaatcaa tccctcatcg acacctacca aagtttgaat atgttgacga gttgcttcat
     6181 aggtcaactg acctaccttt tgcacagcaa tcccatccgc aaatttatca atttccttga
     6241 gtttgactgg acctccagcc tcaaaggcag ctttcatgga acgcgctcca ttcgcctcta
     6301 ctccgataac ctcaatctct ggacttgttt ccttgatata ggtagaaacc ccggcaatga
     6361 gaccgccacc accaacagga accaagacag catcaaaatc aatcgattct tttcgagctt
     6421 cttctaaaat ctcataagca actgttcctt gacctgcttg aacatgagca tcatcaaaag
     6481 gatcaataaa ggtacgattt tcagagactg taaattcttg agctgctttg gctgaggcat
     6541 caaaggtatc tccaactagt ttaatagtta caaaatcccc accaaaaaag cgaacctgac
     6601 caatcttttg ttgtggcgta gtaatgggca taaagatagt agcaggaatt ttcatttcat
     6661 tacaagtata ggctactccc tgcgcatgat ttcccgcaga agcgcagact accccacgtt
     6721 cacgttcttc cttgctgagc tgggaaatgg cataataggc accacgaatt ttaaaggagc
     6781 gaacacgctg ggcattttct tttttcaaat aaatcttagc accatacttc tccgataaat
     6841 aatgatcgta atccagtgga gtattcacaa ccacaccgtt caagaccttg tgagccttga
     6901 ttatatcttt tgaacttaac atataaaacc tctttttcta taaaaaacag tcactaatga
     6961 ctgtttttta gttattcatc tccgaaaact tcagtaacac tttcatggta tttatgagcc
     7021 atacgaatcg aaaactcttt aggaagttca taacgatttg tggaaatcca atcatataac
     7081 cattctacca aactttcttc actatcatta cgatggaaga taaaatagtt taaaatttct
     7141 ttatattcta catcattcaa atccaacgtg tctacaaatt gactacgagg aacaaaatta
     7201 gaatctaatt ttcttagttg atctgtcaat tctcccaatt gtaaatccaa ctgaaaaaat
     7261 agtttttgca gttgcctatc agtttcatca catcctgtct tgatagaaaa caaattggta
     7321 atattcatat gatttcaacc cctaaataag tacatctaat atcccacgaa tagctgaagc
     7381 taaagttttc gcagtccagt catttaaacc cgattctttc aatccagatg caataatttg
     7441 ctctatttga tataaaccgc catctatcgt ctcatttatt ctgcaaagag gaccttcaat
     7501 ataggataaa gcttctccga atctaacagc tgatttaggt cctaatgatt taccaactat
     7561 atttgttaat gttttttatt tctcaaaatc catttcgcta tataacgagc agatttagtc
     7621 gcaccagctc tctcaaacgg agaatgaatt tcaagacctt cttctctagc tatttgtgcc
     7681 aatttgaaga attgttcaaa ggtcatatgc aacctaccat cttcaaaatt agcatcatca
     7741 taaatgttaa ttacagcatt ttcatcatac gaccaatctg ctttattttg gtctgctagc
     7801 actattaccg acggtgtaat tacattatac aactagaacc aataataacg atgcaatttt
     7861 tctcataatc tttttcataa aatcatctcc agcgctatat atttctaatt agttatagat
     7921 tttgaatgca tcatcgtcgt ttttaccaac gaatggcatt gctttacgca attctgcacc
     7981 aactttttca atttcaaggt tagctgcttg ttcacggtaa gcagtcaatt ttggacgtcc
     8041 agctttatag tcatttacaa agtcatttgc aaatttacca ttttggatgt ctgccaagac
     8101 agccttcata ttttctttaa cttgttcagt gattacacgt ggacctgata catagtcacc
     8161 gtattcagca gtgtttgaaa tagattgacg cattttcttg aatccacctt cgtagatcaa
     8221 gtcaacgatc aatttcattt cgtgaagaac ttcaaagtaa gccaattctg gagcgtaacc
     8281 tgcttctgtc aagacttcga aacctgcttc gataagggca gtcaaaccac cacaaagtac
     8341 agcttgttca ccaaacaaat cttcttcagt ttcttctttg taagttgttt caagaagacc
     8401 tacacgagcc gctccaacac ctttacacca gtccatagca atgtttttag catttcctgt
     8461 tgcatcttgg tatactgcat aaagagctgg aacaccaaat ccttcttcgt aagtacgacg
     8521 taccaagtgt cctggtcctt taggagcaca catgaagaca tctacatccg caggaacttt
     8581 gataaattca aagtggatgt tgaaaccatg ggcaaatcca actgcgtttc cagcttccaa
     8641 gtttggagcg atttctgctt cgtacaattc ttgttgaatt tcgtctggcg ccaagatcat
     8701 gataacatca gccaacttag tagcttctgc tactgtgtaa gtatcaaatc catcttcttt
     8761 tgctttatca aaagatttac ctggacgtac accgataata acgtcacgac ctgaatcacg
     8821 caagttttga gcatgcgcat gcccttgtga accataaccg ataacggcga tttttttacc
     8881 gtcaagtgct gctactttaa catctttttc atattccatt tgaactgtca tagcttttct
     8941 ctcttttcta ttatttattg ccttttaggc tggtttaata aatttaagtt ggatttttaa
     9001 tcgcgggtaa atccagttgc acccgttcga gcaatattgc gaataccgta tgggcgaatg
     9061 actcgcaata gggcttcgct cttttctgca tttcccgtca tctgaatggt aatcgagctt
     9121 ggcgctacgt ctaccactgt tgcacggaaa ggttgaataa tcgctaaaat ctcagctctc
     9181 ttctcagctg gcgctgacat cttaaccaaa atcacctcgc gctccaaatg aggcttgtct
     9241 gtaatatctc gaatgcgaat cacatcaatc tgacgattga actgtttgat gatttgctcc
     9301 acttcatcat gagaagcaac atcaataata atagtgatac gcgatacatt cggatcttct
     9361 gttgctccaa cagagatgct ttcaatatta acctgacgac gagataggac acctgtaaag
     9421 cgattgagga ctcctgaacg attttgtaat tttgctgtta acattctacg catggaactt
     9481 cacccccaac atctcatgat tactcttacc agccggtacc attggtaaca cctgttcctt
     9541 acgagaaata tctacctcaa ttagcatagg aacatcctca gtgatgactt caaggtcttg
     9601 agccaaggtc tcaggattgt caaacttata gtttttaata ccataagctt gcgccatcaa
     9661 ttggaaatca ggaagggtat caaagaccga ctctgatgtt ctgccttcat agaaggattc
     9721 ctgccactgg cgaaccattc caagtgaatg attgttcagc ataaccacct tgattggcac
     9781 cttgtaaata ttcaaaatag ccaactcctg gttggtcatt tggaaaccac catccccaac
     9841 aaacaagact acttccttat ctgggttagc aattttagca cctattgctg ctggaattcc
     9901 aaagcccatt gttcccaaac cacctgaagt cactaactga cgttcatttt ggtagggata
     9961 atactgagct gtccacattt ggtgttgacc aacgtctgtt accacaatgg catctccatt
    10021 cgtcaattca ccaattcgtt caataactgc ttgcggttga accacacgct ctttcttgtc
    10081 ataagaacga acacgattct tgtctttagt gactttctca atccactttt cagtattgtt
    10141 gtgaactgtt ggttctgcta gcaacatttg caaggccttc ttagcatctc caactacagg
    10201 aatgtctgca ctgataatct tgccaatctc agctgggtca atatcaatgt gggcaacctt
    10261 agcattctta gcgaaagtct taggattccc cgtcaaacgg tcatcgaaac gagaaccaat
    10321 actaatcata aagtccgctt ccgtcatggc aatatttgct gcgaatgacc cgtgcatgcc
    10381 tcccattcca agaaagagtg ggtgactcgt tgcaatcgtt ccttgtccca aaagactggt
    10441 taccactgga atttgatagc gttctgcaaa ttcatttagt tccgtagcag cctcagcata
    10501 actaattcca ccaccagcta acaagactgg ctttttagcc ttggacaatt gcttcaagat
    10561 tttcttgatt tgcatatcat tcggctcaag agtcggctga taacttggta aattcacttc
    10621 tggtgaataa atgaagtctg tttctaaagc agatatgtct tttggtaggt caattacaac
    10681 tggccctgga cggcctgtag ttgcgatatg gacagcttcc gtaatgatac gcggaatatc
    10741 agctgtctca cgaacttggt aattgtactt agtgattggc atggtaattc ctacgatgtc
    10801 tgcctcctga aaggcatcct tcccaatccc tgctcgcgcc acctgacctg taaagaccaa
    10861 aaggggaacg ctatcgctca tagcatccgc aatccctgta atggcatttg ttgctcctgg
    10921 tccactagtg acgacggcaa cacccaactt tccagttgat ttggcataac cttcagcttc
    10981 atgcaaacaa ccttgctcat ggcgccctag aatgtggcga atgcctttaa aattatatat
    11041 cgcatcataa aaaggcaaaa ccgcaccacc aggataacca aagatggtat caactcctaa
    11101 atcacgaagt gtttccaaaa ctaggtccga ccccgtctta ggagattcta aactgatttt
    11161 ctccattgtt cccctttctt tcctcttaaa aataacttgt tactatcata ccattttttc
    11221 aaaatttttc aagataaaag aagaaatttt ctgaattttc tattttaacg tttatttatg
    11281 aatgttattt ttgtttttat taaaaagata ccgatttcat ttactaaaaa gaaaaaagaa
    11341 ctgatttctc agtccttcat taatcttatt ccacactaaa taggtatggg taaacaggtt
    11401 gttgaccttg gtgaatctcg acttcaacgt cttcgaattc ttctacgatt tcttgagcga
    11461 tttcattggc aagttcttcg cttccgtctt caccgacata gaaggttacg atttcactat
    11521 cttcatccaa catatgtttc aaggtttcag tcaatgtttg gtgcatatca gggtttgaca
    11581 caagaatttt tccatccacc atacctagat tatcgttttc atggatttct aagccatcga
    11641 tcgttgtatc acgcacggct gttgtgacgc ttccgctaac gacatcgcta agagcagctg
    11701 tcatacgctc ttggttttct tcaatggact tgcttggatc aaaggcaaga agacttgtca
    11761 taccttgagg aagagtgcga gcctctacca ctactgctgg ttgctccaaa acttctgccg
    11821 cagattgagc tgccatgaag atgttcttgt tgtttggtaa gaagatgatg ttacgggcat
    11881 tgacctgttc aacagccttg ataaagtctt ctgttgaagg gttcatggtt tgaccgcctt
    11941 cgataacata atccacgcct tgagaacaga agatatctgc tagaccttta ccagccacca
    12001 cagcaatcaa agcatactct ttttcttcag ccgacttgat aacttgagta gcttctttct
    12061 caacctgtgc ttcgtgttgg ttacgcatat tgtcaacttt taccttgacc aagctaccat
    12121 atttgagacc ttcttgcata acaagtcctg gatcttctgt atgaacatgg actttgacaa
    12181 tttcatcatc gttaacaacg aggagagaat ccccaagctc atccaagtag ttacggaatt
    12241 catcgtagtc aaaatcttta gcataggttg gaccttgctt aagagctacc atgatttcag
    12301 tacagtaacc aaacgtgatg tcctcagttg ctacgtgacc agctacagac ttatgatgct
    12361 ctacattgat catctcactc atgttggcag gagtcgctac aaagtcctca gatgcaatat
    12421 attcgccagt aagggctgaa aggaaacctt cgtagatgaa gaccaatcct tgaccacctg
    12481 agtccacaac gccaacttct ttcaatactg gaagcatgtc tggtgtttta gctagagctg
    12541 ttttagcacc ttccaaggct gcgcgcatga cttcaacagt gtcatctgtt tgctcagctt
    12601 ttttcttagc accgatagca gctccacgag aaactgtcaa aatcgttcct tcaacaggtt
    12661 tcatcactgc cttataggca acttccacac ctgattggaa ggccagagcc aagtcttgac
    12721 ctgttaactc gtctttatcc ttgatagctt gggaaaatcc acggaaaagc tgagacgtaa
    12781 tcactcctga gttcccacgc gcacccatca aaagcccttt ggcaagaatg ctcgctactt
    12841 ctccaactgt agaagctggc ttgtctgcaa cttctttagc accattttca atggtcattc
    12901 ccatatttgt cccagtatct ccatctggaa ctggaaagac gtttaatgaa ttgacatatt
    12961 cagcttgctt attcaagcga gttgatgcag cctgcaccat ttcttgaaat aagctagtag
    13021 taatttttga cacggttatt ctcctacaac tttgatattt tgaatgtaga catttacagt
    13081 ctgagcagta attccaagct ggttttccaa gctaaaggca acacgctctt gaatgttttt
    13141 tgacacttca ctaatctttg ttccgtagct taacacggta tatacatcaa ctgcaatact
    13201 gccatcttcg gctgccttta cgacgacacc tttagaataa ttttccttac ctagcagggc
    13261 ttggaaatta tctttgaggg catttttact agccataccg accacaccaa aaatctcagt
    13321 tgctgctcca cctacgacgg ttgcaatcac ttcatctgtt agttcgattt gaccatcttt
    13381 tgtattaatt tttacagtca tcctttttac ctcaactagt tgatactcta ttttatcata
    13441 tttcagccca agtgtaaaag cagaatgatg tatcagcgga tatttactct atttttcaaa
    13501 cgatgttata cccacaataa aagaaaaaag accctaaggt ctcctttgct tttattatta
    13561 aacgcgttca actttacctg atttcaaagc acgagctgaa gcccaaactt ttttaggttt
    13621 accatcgata agaacagtaa ctttttgaag gtttggtttt acggcacgtt ttgtttggtt
    13681 catcgcgtgt gaacggttgt ttcctgatac agtcttacga cctgtaaagt aacatacttt
    13741 agccattgtg ttttcctcct attagatcta atatagcgga tgtgctagca ccacataccg
    13801 tactatgtta tcacactttc ttgttttttg caagggaatt ggaagatttt ttatttgtgt
    13861 cttaaatcag gtcttgcgtg acattttctg ctctccacat gccatcgttg attaacagaa
    13921 caccagaatt aaaattatgt gtataaaaat catctctaac tgcagctaag ggtatagccg
    13981 tcaagtccaa atcccacagc tcatctatcg attttcttac aacaatatct gaatccaaat
    14041 acagtacacg agactcgctt acatactttg gaataaaata cctaaaaaag ccgcatatga
    14101 aagtccctca aaggggagac gataaccttt cagaatatta ctgtcaatct aaacattcac
    14161 aatctcacta ttcaaagtct ctagtctttt ttccatcaat tggaaccatt ctcgcggaag
    14221 gtcatcatta aaaacataaa acttaagatt ataatgatga acacaaagag attttattgt
    14281 tgtttcaact ttatccatat aagcattatc tgcacctaag acaatcgctt ttttctcttc
    14341 tttcactttt tatctcattt ctttttattc ccatcatatg tttctacgta accattattt
    14401 tcgcctattc gttcgtaaaa ccataccagt ggagatttta gatgaagtcc cattacggtt
    14461 tacaattttt acattacgac acggagtttt acaaatcgat ttcatttgcc aaacgtagtt
    14521 agtgaggcag ttagctagtt cgccaaatag cgactagcgt ccaacaattt ggaactttag
    14581 ttccaattgt tggtactgag tcacatcttc tcctctaact ctacgtctgg atacttgtca
    14641 gcaaaccagc ggagggcaaa gtcattttca aagagaaaga ctggttggtc aaaacggtct
    14701 ttggctaaga tattgcgact tgacgacatc cgttcatcca agtcctcagg cttgatccaa
    14761 cgaacggtct ttttacccat tgggttcata actacttctg cattgtactc gccttccatg
    14821 cggtgtttaa agacttcaaa ctggagttga cctacagcgc ctagcatgta ctcacctgtt
    14881 tggtaattct tataaagctg aacggctcct tcttgcacca attgctcaat ccccttgtgg
    14941 aaggattttt gcttcataac attcttagca gaaactttca tgaaaatttc aggagtaaag
    15001 gttggcagtg gttcaaattc aaacttgttt ttaccaactg tcaaggtatc cccaacttga
    15061 taagtaccgg tatcgtaaac cccgataata tcacctgcca cggcattgat cacattctca
    15121 cgactttccg ccataaactg agtaacatta gacagtttag cccccttacc agtacgaggg
    15181 agattgacac tcatgccgcg ctcaaattcg ccagatacga tacggacaaa ggcaatacgg
    15241 tcacggtgac gagggtccat gttggcttgg attttaaaga caaagcctga gaaatccttg
    15301 tcataaggat ccacaatttc accgtctgtt ttcttgtgac catgtggttc tggagcaaac
    15361 ttgaggaagg tttcaaggaa ggtctgcaca ccaaagtttg tcagggctga accgaaaaag
    15421 acaggcgtca attctccagc cagaatagct tcctctgaaa actcattccc ggcttcattt
    15481 aaaagctcaa tatcatcctt gacttgctcg tagaaaggat tgctaccaaa aagtttgtcc
    15541 ccatcttcta gactggcaaa acgctcatcc cctttataaa gctctaaacg ttggttatag
    15601 aggtcataca agccctcaaa ggctttcccc atcccgatag gccagttcat agggtagcta
    15661 gcaatgccca agatttcttc caattcttgc aagagatcca aaggctcacg accgtcacgg
    15721 tccagcttgt tcataaaggt aaagactgga atgccacgat gtttcacaac ctcaaacaat
    15781 ttcttggttt gagcctcgat ccccttggca gagtccacga ccatgaccgc agcatccacc
    15841 gccatcaagg tacgataggt atcttctgag aagtcctcgt gccctggcgt gtctaagata
    15901 ttcacgcgct tgccgtcgta gtcaaattgc ataacagatg aagtaacaga aatcccacgt
    15961 tgcttctcga tatccatcca atcagattta gcaaaagtcc ctgttttctt cccttttacc
    16021 gtaccagcct cacgaatttc acccccaaag tagagtaact gctcagtgat ggttgttttc
    16081 cccgcgtccg ggtgggagat aatggcaaag gtacgacgtt tcttaatttc ttcttgaata
    16141 ttcataagtt ctctttcttt gattctctat ttttcttgtt tcaatagctg agaatgattt
    16201 ttacattgga ttttaccatt cctttcaaca ctccattata tcggatttta gcattttttt
    16261 caatttctat ttcttttcac ttccccctcc cttatttata ggaaaatatg gtaaaataga
    16321 acagactaaa aatcatcatt tcacgaaagg atgcaagatg aaaattacgc aagaagaggt
    16381 aacacacgtt gccaatcttt caaaattaag attctctgaa aaagaaactg ctgcctttgc
    16441 gaccaccttg tctaagattg ttgacatggt tgaattgctg ggcgaagttg acacaactgg
    16501 tgtcgcacct actacgacta tggctgaccg caagactgta ctccgccctg atgtggccga
    16561 agaaggaaca aaccgtgatc gcttgtttaa aaacgtacct gaacaagaca actactatat
    16621 caaggtgcca gctatcctag acgatggagg agatgcctaa tgacttttaa caataaaact
    16681 attgaagagt tgcacaatct ccttgtctct aaggaaattt ctgcaacaga attgacccaa
    16741 gcaacacttg aaaatatcaa gtctcgtgag gaagccctca actcatttgt caccatcgct
    16801 gaggagcaag ctcttgttca agctaaagcc attgatgaag ctggaattga tgctgacaat
    16861 gtcctttcag gaattccact tgctgttaag gataacatct ctacagacgg tattctcaca
    16921 actgctgcct caaaaatgct ctacaactat gagccaatct ttgatgcgac agctgttgcc
    16981 aatgcaaaaa ccaagggcat gattgtcgtt ggaaagacca acatggacga atttgctatg
    17041 gggggttcag gtgaaacttc acactacgga gcaactaaaa acgcttggga ccacagcaag
    17101 gttcctggtg ggtcatcaag tggttctgcc gcagctgtag cctcaggaca agttcgcttg
    17161 tcacttggtt ctgatactgg tggttccatc cgccaacctg ctaccttcaa cggaatcgtt
    17221 ggtctcaaac caacctacgg aacagtttca cgtttcggtc tcattgcctt tggtagctca
    17281 ttagaccaga ttggaccttt tgctcctact gttaaggaaa atgccctctt gctcaacgct
    17341 attgccagcg aagatgctaa agactctact tctgctcctg tccgcatcgc cgactttact
    17401 tcaaaaatcg gccaagacat caagggtatg aaaatcgctt tgcctaagga atacctcggt
    17461 gaaggaatta acccagaggt taaggaaacc attctaaatg ccgctaaaca ctttgaaaaa
    17521 ttgggtgcta ttgtcgaaga agtcagcctt cctcactcta aatacggagt tgccgtatac
    17581 tacatcatcg cttcatcaga agcttcatca aacttgcaac gcttcgacgg tatccgttac
    17641 ggctatcgcg cagaagatgc aaccaacctt gatgaaatct atgtaaacag ccgaagccaa
    17701 ggttttggtg aagaggtaaa acgtcgtatc atgctgggta ctttcagtct ttcatcaggt
    17761 tactatgatg cctactataa aaaggctggt caagtccgta ccctcatcat tcaagatttc
    17821 gaaaaagtct tcgcggatta cgatttgatt ttgggtccaa ctgctccaag tgttgcctat
    17881 gacttggatt ctctcaacca tgacccagtt gccatgtact tagccgacct attgaccata
    17941 cctgtaaact tggcaggact gcctggaatt tcgattcctg ctggattctc tcaaggtcta
    18001 cctgtcggac tccaattgat tggtcccaag tactctgagg aaaccattta ccaagctgct
    18061 gctgcttttg aagcaacaac agactaccac aaacaacaac ccgtgatttt tggaggtgac
    18121 aactaatgaa ctttgaaaca gtcatcggac ttgaagtcca cgtagagctc aacaccaatt
    18181 caaaaatctt ctcacctact tctgcccact tcggaaatga tcaaaatgcc aacactaacg
    18241 tgattgactg gtctttccca ggagttctgc cagttctcaa taaaggggtt gtcgatgccg
    18301 gaatcaaggc tgctcttgcc ctcaacatgg acatccacaa aaagatgcac tttgaccgca
    18361 agaactactt ctatcctgat aaccccaaag cctaccaaat ttctcaattt gatgaaccaa
    18421 tcggatataa tggctggatt gaagtcgaac tagaagacgg tacgaccaag aaaatcggta
    18481 tcgaacgtgc ccacctagag gaagacgctg gtaaaaacac ccatggtaca gatggctact
    18541 cttatgttga cctcaaccgc caaggggttc ccttgattga gattgtatct gaggcagata
    18601 tgcgttctcc tgaagaagcc tatgcttatc tgacagccct caaggaagtt atccagtacg
    18661 ctggcatttc tgacgttaag atggaggaag gttcgatgcg tgtggatgcc aacatctccc
    18721 ttcgtcctta tggtcaagag aaattcggta ccaagactga attgaagaac ctcaactcct
    18781 tctcaaacgt tcgtaaaggt cttgaatacg aagtccaacg ccaggctgaa attcttcgct
    18841 caggtggtca aatccgccaa gaaacacgcc gttacgatga agcgaataaa gcaaccatcc
    18901 tcatgcgtgt caaggaaggg gctgctgact accgctactt cccagaacca gacctacccc
    18961 tctttgaaat ttctgacgag tggattgagg aaatgcggac tgagttgcca gagtttccaa
    19021 aagaacgtcg tgcgcgttat gtatctgacc ttggtttatc agactacgat gctagtcagt
    19081 tgactgctaa taaagtcact tctgacttct ttgaaaaagc tgttgcccta ggtggtgatg
    19141 ccaaacaagt ctctaactgg ctccaagggg aagtcgctca gttcttgaat gctgaaggta
    19201 aaacactgga acaaatcgaa ttgacaccag aaaacttggt tgaaatgatt gccatcatcg
    19261 aagacggtac tatttcatct aagattgcca agaaagtctt tgtccatcta gctaaaaatg
    19321 gcggtggcgc gcgtgaatac gtggaaaaag caggtatggt tcaaatttca gatccagcta
    19381 tcttgatccc aatcatccac caagtctttg ccgataacga agctgctgtt gccgacttca
    19441 agtcaggcaa acgtaacgcc gacaaagcct ttacaggatt ccttatgaag gaaaccaaag
    19501 gccaagccaa cccacaagtt gcccttaaac tacttgcaca ggaattggcg aagttgaaag
    19561 aaaactagac agaacaaaac cagccctaag gttggttttt tcttctctac caactcccaa
    19621 taactatttt ggctttattt ccagagtatt ttatggtaaa atgaagagta ataatattta
    19681 ttaaagaggt aaaaacatga ttgaagcaag taaattaaaa gctggtatga cctttgaaac
    19741 agctgacggc aaattgattc gcgttttgga agctagtcac cacaaaccag gtaaaggaaa
    19801 cacgatcatg cgtatgaaat tacgtgatgt ccgtactggt tctacatttg acacaagcta
    19861 ccgtccagag gaaaaatttg aacaagctat tatcgagact gttccagctc aatacttgta
    19921 caaaatggat gacacagcat acttcatgaa tacagaaact tacgaccaat acgaaatccc
    19981 tgtagtcaat gttgaaaacg aattgcttta catccttgaa aactctgatg tgaaaatcca
    20041 attctacgga acggaagtga tcggtgtcac cgttcctact actgttgagt tgacagttgc
    20101 tgaaactcaa ccatctatca aaggtgctac tgttacaggt tctggtaaac cagcaacgat
    20161 ggaaactgga cttgtcgtaa acgttccaga cttcatcgaa gcaggacaaa aactcgttat
    20221 caacactgca gaaggaactt acgtttctcg tgcctaatct ctagaaagag gtcattctat
    20281 gggaattgaa gaacaacttg gcgaaatcgt tatcgcccca cgtgtacttg aaaaaatcat
    20341 tgctatcgct actgcaaagg tagagggggt tcactctttt tcaaacagat cagtgtctga
    20401 taccctttca aaactttcac tcggccgtgg catttatctt aaaaacgtgg acgaagaact
    20461 cacagcagat atctatctct accttgagta cggagtaaaa gttcctaagg tagcggttgc
    20521 tatccagaaa gctgtcaaag atgccgtccg taatatggct gatgtagaac tcgctgctat
    20581 caatattcac gttgcaggta tcgtcccaga taaaacacca aaaccagaat tgaaagatct
    20641 atttgacgag gacttcctca atgactagtc cactattaga atctagacgc caactccgta
    20701 aatgcgcttt tcaagctctc atgagccttg agttcggtac ggatgtcgaa actgcttgtc
    20761 gtttcgccca tactcatgat cgtgaagata cggatgtaca acttccagcc tttttgatag
    20821 acctcgtttc tggtgttcaa gctaaaaagg aagaactaga taagcaaatc actcagcatt
    20881 taaaagcagg ttggaccatt gaacgcttaa cgctcgtgga gagaaacctc cttcgcttgg
    20941 gagtctttga aatcacttca tttgacactc ctcagctggt tgctgttaat gaagctatcg
    21001 agcttgcaaa ggacttctcc gatcaaaaat ctgcccgttt tatcaatgga ctgctcagcc
    21061 agtttgtaac agaagaacaa taaaaagaaa ggctctataa tatttgtagt gggtaaatcc
    21121 cctatggata ttatggagcc tatttttgtg tagaaaaaaa gtcccataag atctataatg
    21181 aaaagcgatc aaacaactca ttagaaagat tcatatggaa caattacatt ttatcacaaa
    21241 actactcgat atcaaagaca caaatatcca aattatagat gtcgtcaata gggattcaca
    21301 caaagaaatc atcgccaaac tggactacga cgcaccatct tgccctgatt gtggaagtct
    21361 aatgaagaaa tatgactttc aaaaaccgtc taagatccct tacctcgaaa caactggtat
    21421 gccttctaga attctcctta gaaaacgccg tttcaagtgc tatcactgtt caaaaatgat
    21481 ggttgctgag actcccctgg taaagaaaaa tcaccaaatc cctcgtatca tcaaccaaaa
    21541 aattgctcaa aagttaattg aaaaaatttc tatgactgat attgcccat
//
LOCUS       DAVKHZ010000029        21381 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000029 DAVKHZ010000000
VERSION     DAVKHZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.029"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            515..1379
                     /locus_tag="VWW99_001024"
                     /pseudo
     CDS             515..1379
                     /locus_tag="VWW99_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
     gene            1670..1867
                     /locus_tag="VWW99_001025"
     CDS             1670..1867
                     /locus_tag="VWW99_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9538881.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            1935..2600
                     /locus_tag="VWW99_001026"
     CDS             1935..2600
                     /locus_tag="VWW99_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9538882.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            2672..3898
                     /locus_tag="VWW99_001027"
     CDS             2672..3898
                     /locus_tag="VWW99_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9538883.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLKHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            4351..5007
                     /locus_tag="VWW99_001028"
     CDS             4351..5007
                     /locus_tag="VWW99_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9538884.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIAVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            4997..5320
                     /locus_tag="VWW99_001029"
     CDS             4997..5320
                     /locus_tag="VWW99_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9538885.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            5424..6254
                     /locus_tag="VWW99_001030"
     CDS             5424..6254
                     /locus_tag="VWW99_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9538886.1"
                     /translation="MKKIVRYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKIFDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPSEADIK
                     "
     gene            6295..6372
                     /locus_tag="VWW99_001031"
                     /pseudo
     CDS             6295..6372
                     /locus_tag="VWW99_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            6476..7306
                     /locus_tag="VWW99_001032"
     CDS             6476..7306
                     /locus_tag="VWW99_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9538887.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            7460..8314
                     /locus_tag="VWW99_001033"
     CDS             7460..8314
                     /locus_tag="VWW99_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9538888.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            8414..9787
                     /locus_tag="VWW99_001034"
     CDS             8414..9787
                     /locus_tag="VWW99_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9538889.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            9780..10841
                     /locus_tag="VWW99_001035"
     CDS             9780..10841
                     /locus_tag="VWW99_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9538890.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILTLLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            10843..11535
                     /locus_tag="VWW99_001036"
     CDS             10843..11535
                     /locus_tag="VWW99_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538891.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11565..12134)
                     /locus_tag="VWW99_001037"
     CDS             complement(11565..12134)
                     /locus_tag="VWW99_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9538892.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            12269..12883
                     /locus_tag="VWW99_001038"
     CDS             12269..12883
                     /locus_tag="VWW99_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9538893.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12885..14531
                     /locus_tag="VWW99_001039"
     CDS             12885..14531
                     /locus_tag="VWW99_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9538894.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14543..15829
                     /locus_tag="VWW99_001040"
     CDS             14543..15829
                     /locus_tag="VWW99_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538895.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15881..16414
                     /locus_tag="VWW99_001041"
     CDS             15881..16414
                     /locus_tag="VWW99_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538896.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            16492..16962
                     /gene="nrdI"
                     /locus_tag="VWW99_001042"
     CDS             16492..16962
                     /gene="nrdI"
                     /locus_tag="VWW99_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9538897.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(17255..18517)
                     /locus_tag="VWW99_001043"
     CDS             complement(17255..18517)
                     /locus_tag="VWW99_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9538898.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18827..19285)
                     /locus_tag="VWW99_001044"
     CDS             complement(18827..19285)
                     /locus_tag="VWW99_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9538899.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(19282..19722)
                     /locus_tag="VWW99_001045"
     CDS             complement(19282..19722)
                     /locus_tag="VWW99_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9538900.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            20155..21021
                     /locus_tag="VWW99_001046"
     CDS             20155..21021
                     /locus_tag="VWW99_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9538901.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
BASE COUNT         6387 a         3842 c         4664 g         6488 t
ORIGIN      
        1 gaaaagaatt ttgaactatt tacaaaacaa agtgatctaa ttgttgcaaa tagatgggat
       61 aggggccttg aagcttataa agataaagtt tacacaaggg ggatctggat aagagattaa
      121 ctattgatat taaaaaggca ataatttata aaatttgtac aaaaaaatgt taaattttca
      181 cattttcata gtatactgtt taatagaaag taggttcttt gtaaccgctc cattataaaa
      241 tcctttcact cttccggtat actattagga aaaacataac tggaggatat tttgagctca
      301 cacctcatcg ttccagtggc tgttccccaa gctcgtgtac tgcccaaaaa agttagacat
      361 tctatttaga acgaggattg agttctgtat tgtacaaggc tcggtccttt tagagtcagc
      421 ttaaggctgg ctttttcaat caccaaagtg tcagaatgtt ttgacaaatg aacacaaata
      481 atgatataat atgcaaaagc taggaggtgg taggatgatt gaaaaaatgg aactggggga
      541 attttacaag gaattacgtt tggcgagaaa gcttaagcag acagatgtgg cttgtgaggg
      601 tctaacagct tctcagttgt ccaagtttga actagggcag tctatgctgt ctgcggataa
      661 gttgattcta gctatccaag gtataaatgt gaccattgat gagtttgggc acaagctcaa
      721 caactatcaa gaatctccac atatgcgaat cggtcgaaag gttgtgaatc gctttgccca
      781 tcaggatata gctgctttag agcagctatt ggaggaagtc gatcaagaac agatggcgca
      841 gacctatcgt cgtttgaatg ccatcgtgat taaagatgcc atccattcac taaataaaag
      901 ctacccacta gcagaggagg atagcgagtt tttgaccacc tatctctatg ctatcgagtc
      961 ttggacctgg tttgaactct atctcttttg caataccatg cccttcttga gcaatcaaga
     1021 tctgattttt tttatcaacc tccttactcg aaaaatccaa agaatttaaa gagttagtac
     1081 acaatcgatt gtatatgaag caaggactct taaatatctt atcagaactc atggagcgaa
     1141 aacttttctc ctacatccca atcttcgaag ccgagttgga gaggatgtta cgaccgtatg
     1201 atgtttttga aaaagtatcg tggcaatttt taaagaagat gagtgtcttt cttcaaacaa
     1261 agggaagcaa tcaaaaagag attgaacgct ttatccaatc tctgcaggta ttagaaaatc
     1321 cacaattaac atcccttttt gaattgcgtt ttcagcaata taaagaactt atagattagt
     1381 taaaaatgga atgacaataa cccactccga aataagtagg agtatcagtt ggatttttct
     1441 tttcttcatc tattatattc ctccttgtta gtaataactt tattatacca gggaaataat
     1501 caaatctatc aaaatcgcaa ataagaaatt tctataagaa aaaatatcaa atatgcgatt
     1561 ttttaaaata agccaatttt cgtgttatac tgtacttgta aagcacttga agcaaatcct
     1621 aggtcgcaga agtggtttac aaatgaagat aattgaagga gtgtaaaaga tgttaaattt
     1681 acaatttgca gaaacaatgg aattgacaga agctgagttg gagacagttt atggagggga
     1741 atttgggaat aatgctgtta tcccagccgg tgcttgggga ggtttaggaa cgtcttggtc
     1801 aatcactaat ttctggaaga aatattttaa ccatgattct tccactgtta atcgtcgcca
     1861 ttattgatat tcagagtcct tgcctttttc ttgggtaggg actgtcttta tttcatagta
     1921 taggagaaaa tagaatgaaa aagataatct cacaccgcta ctttattata gtttttctac
     1981 tagttattgc tgaccagaag ttcagtgttt tagttttacg cagcgacctt gttgctgggc
     2041 tatctgactt tgcctattat ctgtcggata tgatgttgaa ttttcttgtg gttttatttg
     2101 ctcttattgc tatgatttgg tcggggaaat ggcagaaaat caatagtaga aagtttaaag
     2161 gatcctatct tttctattcc ttcctagctc tgcttgcttt tgttgcttgg aattttgtca
     2221 ccttttttct tttcccacct actcgaaatg aaatttctta tcaacatgct gctcctactt
     2281 ttacaggagc tacggcattt ttgatgtatt ttttttatcc tgtgattgca ggtcccattt
     2341 ttgaagacat gatttatcgt ggattagtga tgactgctct ggaaaaagga aagaaatggg
     2401 ggctagatgt acttggttcc gctgttttat ttggggtctc gcacattagt aatcacggtt
     2461 gggtcttgac agactttgtc ttctatatgg gtggaggtct tatatttgca gtcttattta
     2521 gaatgacaaa gtcaatttat tggcctattg gactgcatat agtctacaac ggcattggtc
     2581 agcttttgat gttactgtaa ttttggttgt tagtagtatc gcggtctatt tctggggaaa
     2641 gattcccatt tgaacttaag aaggagttgt gatgaaagta tttcttcaaa atagagattt
     2701 taggcaatta actatcaacc agtggatttc aacgcttggg gatacgattt tttatctggc
     2761 ctttctgaat tatgtggcag atgcatcttt tgcccctttg gcgattttac tcatcacgat
     2821 ttcagaaacc ctgccccaag ttctacaaat ctttctggga gttttggcgg attttcaaca
     2881 tcatcgtgtc ttgaaataca cagtcattag ttttgcaaaa tttttgcttt actctatagt
     2941 gtctttatca ctttcagggc agtccttttc cttgttgtta gtagcattta tttgtctgat
     3001 taacctcttg tctgacacgt tgagttattt ttcaggcgcc atgctcactc cgattttcat
     3061 tagaattatt gggcaagacc atctggcaga agctattgga tttaaacagt caactgttag
     3121 tttagtgaaa acaatcagta atattctagg aggagtctta ttaggtattc tatccatcca
     3181 gtttatttcc ttgctgaacg ctcttacctt tttaatcgca tttttaggta tcctcttcat
     3241 aaaaactgac ctcttgaaag tagaaaaaac gattaactat caagaaggac tctctgtaaa
     3301 atccttttgc cagcatttgc tccaatcatc gaaattgata tggaatatga ataaggtgct
     3361 cttggttttg tttattatct ctactagtca agcagtgata aatgttacgg ttcctatttc
     3421 cactctattt ttaaggaacc agcccttttt gaatttacaa acaggtcaat ctcttgcctt
     3481 gctatccact tttgaactgt cagccctgat tgtcggaagc cttgtaagtg gctatctgaa
     3541 acatacgatt tctataaaaa cagctctcta tgcctcactt gtcatccagt tgcttcttct
     3601 agtaggattt gccacagttc gttttgactg gattctcatc tttagtacct tggatgcctt
     3661 tttcgcaggt gttctctctc ctagattgca agaactcgtt tttaaacaaa tacctgagga
     3721 gtcaatggga gcagttcaat cttctatcgg tgccattacg gttgtcttac ctagcttatt
     3781 tacaatagct ttggtaacca ttgctactag ctttggaact ctggcagtta gctttgtttt
     3841 attgctattt cttctagttg cctttgttat gcttctgaat attcgtgaaa gtatttagtg
     3901 gagaatttct ataatatatg tttgagactg gattgccagt ctttttatga tggtcaaaat
     3961 caacagactt tttctctttt ctgaatgtgt tatagtagac tgaatctaaa atagtacgaa
     4021 acaattgcta aaacatttat agaaattaat tttactttcc caatcgattt gttctcatct
     4081 tatttcaatc tgctatataa tagtccatgc tgtgactgga ttcttttcag cctacttata
     4141 ctcttcgaaa ctctcttcaa accacgtcag cttcaccttg ccgtatatat gttactgact
     4201 tcgtcagttc tatccacaac ctcaaaacgg tgttttgagc aacctgcggc tagtttccta
     4261 gtttgctctt tgattttcat tgagtattat taagacaata tgaggagaaa gatgaaagaa
     4321 aaaggatttt aggagggggc gcaggcagcc atgccaacgg ctcttggcta tgtcagtatc
     4381 ggcctggcct gtggaattat cggtgcgccc tatgtgacac ctgttgagat gggcttgatg
     4441 agtctctttg tttatgctgg gagtgcccag tttgccatgt tggcactgat tgcggttcaa
     4501 gctcctgtgg cagctattgc tatgacggtt tttctaatca atttgcgtct ctttttgttg
     4561 agtttacacg catcgactta tttccgtcat accagtcttt ggtacaatat cggtatgtct
     4621 agtatcttga cagatgagac ctatggcgtt ttgatgggcg aattagccca tacagataag
     4681 gtcaatccga tgtggatgca cgggaacaat cttaacagct atgtggcttg gtttgtggga
     4741 acagtggtcg gaacggctct aggtgacctg ctgccaaatc cagaaatctt tggtttagat
     4801 tttgccctgg ttgggatgtt tattggtatt tttgcttcgc aatttcagat gatgcaaaga
     4861 cggattcctg tccgcaatct gctcattatc ctagcagttg ttgcggtgtc cttctttttg
     4921 ctcttgacag tggtgtctca gtcactagct gttctgtttg cgacgctact tggttgtagc
     4981 atgggggtgg ttttagatgg tcagtaagta tcttttatta gcagttattt tctctggctt
     5041 ggtgacttgg attccccgta tgattccctt catcttagtc aagtataagg gcttgcctgc
     5101 aatcgttgag cgttttttga agttcttgcc cgtttccatt atctttgcct tgattctttc
     5161 aagcgtagtg acaggtaagg ttgggagcct tcctcaaatt aaatggctag acttcttagc
     5221 cgtctttcca acagcttggg tagcctttcg ctaccgcaat ctagtcggaa cagttctctt
     5281 tggagtagtc ttgattgccg tcttgcgttt ggtcttttaa atcagccata aagaaaacct
     5341 atcacagata tatatatcat ataatggtgt aaatgatttt tttctgttaa gattataagg
     5401 tattctattt tggaggaaat gacatgaaaa aaatcgttag atactcatct cttgctgccc
     5461 tagggcttgt tgctgcaggt gtgctagcag cttgctcagg tggtgctaag aaagaaggac
     5521 aagcagctag caagaaagaa attattgttg caactaattc ttcaccaaaa ccattcaact
     5581 atgaagaaaa tggcgaattg actggttatg agattgaagt cgttcgcgct atctttaaag
     5641 actctgacaa gtatgatgtc aagtttgaga agacagagtg gtcaggtgtc tttgcgggtc
     5701 ttgacgctga ccgttaccaa atggctgtta acaacatcag ctacactaaa gaacgtgcgg
     5761 agaaatacct ttatgcagcc ccaactgtta aagaccctaa cgttcttgta gtcaagaaag
     5821 acgattctag catcaaatcg cttgatgatg ttggtggaaa atcaacagaa gttgttcaag
     5881 gaacaacatc tgctaaacag ttagaagact acaacaaaca acactcagat aatccaacta
     5941 tccttaacta tactaaagca aacttacaac aaatcatggg ccacttgagc gatggtcagt
     6001 ttgactacaa gatttttgat aaaatcactg ttgaaacagt catcaagaac caaggtttgg
     6061 acaacttgaa agtcatcgat cttccaagcg accaacaacc ttacgtttac ccacttcttg
     6121 ctaaaggtca agatgagttg aaaacatttg tagacaaacg tatccaagaa ctctacaaag
     6181 acggaactct tgaaaaattg tctaaacaat tcttcggaga cacttaccta ccgtcagaag
     6241 ctgatattaa ataatttctt gaaatcatcc attctgagta agatgggtga tttttcagtt
     6301 cgctaccgca atctagtcgg aacagttctc tttggagtgg tcttgattgc catcttgcgt
     6361 ttggtctttt aaattaccca taaaaaaaac ttatcacaga gatagatatc atataatggc
     6421 gtaaatgctc tttttctgtt aagattataa ggtattctat tttggaggaa atgacatgaa
     6481 aaaaatcgtt aaatactcat ctcttgctgc cctaggactt gttgctgcag gtgtgcttgc
     6541 ggcttgctca gggggtgcta agaaagaagg agaagcagct agcaagaaag aaatcatcgt
     6601 tgcaaccaat ggatcaccaa ggccatttat ctatgaagaa aatggcgaat tgactggtta
     6661 cgagattgaa gtcgttcgcg ctatctttaa agattctgac aaatatgatg tcaagtttga
     6721 aaagacagaa tggtcaggtg tctttgctgg tcttgacgct gaccgttaca atatggctgt
     6781 caacaatctt agctacacta aagaacgtgc ggagaaatac ctctatgccg caccaattgc
     6841 ccaaaatcct aatgtccttg tcgtgaagaa agatgactct agtatcaagt ctctcgatga
     6901 tatcggtgga aaatcgacgg aagtcgttca agccactaca tcagctaagc agttagaagc
     6961 atacaacgct gaacacacgg acaacccaac tatccttaac tatactaagg cagatttcca
     7021 acaaatcatg gtacgtttga gcgatggaca atttgactat aagatttttg ataaaatcgg
     7081 tgttgaaaca gttatcaaga accaaggttt ggacaacttg aaagttatcg aacttccaag
     7141 cgaccaacaa ccgtacgttt acccacttct tgctcaaggt caagatgagt tgaaatcatt
     7201 tgtagacaaa cgcattaaag aactctacaa agacggaacg cttgaaaaat tgtctaaaca
     7261 attcttcgga gatacttatc taccagcaga ggctgatatt aaataatttc ttgaaatcat
     7321 ccattctgag taagatgggt gatttctcag tttttaggga tatagtttta aactatatat
     7381 ctgattatct aataacaatt tgtgaaatca aacaaattgt gagatactag tacggtatta
     7441 tttttaagga gaaagaatca tgaaaatcaa aaaatggctt ggcctagtag cccttgctac
     7501 agtcgcaggt ttggctcttg cagcttgcgg aaactcagaa aagaaagcag acaatgcaac
     7561 aactatcaaa atcgcaactg ttaaccgtag cggttctgaa gaaaaacgtt gggacaaaat
     7621 ccaagaattg gttaaaaaag acggaattac cttggaattt acagagttca cagactactc
     7681 acaaccaaac aaagcaactg ctgatggcga agtagatttg aacgctttcc aacactataa
     7741 cttcttgaac aactggaaca aagaaaacgg aaaagacctt gtagcgattg cagatactta
     7801 catctctcca atccgccttt actcaggttt gaatggaagt gccaacaagt acactaaagt
     7861 agaagacatc ccagcaaacg gagaaatcgc tgtaccgaat gacgctacaa atgaaagccg
     7921 tgcgctttac ttgcttcaat cagctggctt gattaaattg gatgtttctg gaactgctct
     7981 tgcaacagtt gccaacatca aagaaaatcc aaagaatttg aaaatcactg aattggacgc
     8041 tagccaaaca gctcgttcat tgtcatcagt tgacgctgcc gttgtaaaca ataccttcgt
     8101 tacagaagca aaattggact acaagaaagc acttttcaaa gaacaagctg atgaaaactc
     8161 aaaacaatgg tacaacatca ttgttgcaaa aaaagattgg gaaacatcac ctaaggctga
     8221 tgctatcaag aaagtaatcg cagcttacca cacagatgac gtgaaaaaag ttatcgaaga
     8281 atcatcagat ggtttggatc aaccagtttg gtaataagaa acagggaggt gggagagaaa
     8341 attccacctc ttgcttttgt atagagtata gattgtaaag aagactattc gttcatagaa
     8401 aggtagagag aatatggttt ttcctagcga acaagaacag attgaaaaat ttgaaaagga
     8461 tcatgtagtc caacattatt ttgaggtttt gcgtaccttg atttctaaga aatcagtctt
     8521 tgcccagcag gttggactca aggaagtcgc aaattatctg ggtgagattt tcaagcgtgt
     8581 tggagctgaa gtggagattg atgagagcta tacagcgccc tttgtcatgg cacatttcaa
     8641 gagttcgcgt ccagatgcca agaccttgat tttctataac cactatgaca ctgtgccagc
     8701 ggatggggat caggtctgga cagaggatcc ttttacgctt tcggtccgca atggcttcat
     8761 gtatgggcgt ggggttgatg acgacaaggg tcatatcaca gctcgcttga gtgctttgag
     8821 aaaatatatg cagcaccatg atgatttacc tgtcaatatc agctttatca tggagggagc
     8881 ggaggaatcg gcttcaacag acctagataa gtatttggaa aagcatgcag acaaactccg
     8941 tggggcggat ttgttggtct gggaacaagg gaccaaaaat gccttggaac agctggaaat
     9001 ttctggtggc aataagggga ttgtgacctt tgatgccaag gtaaaaagcg ctgatgtgga
     9061 tatccactcg agttatggtg gtgttgtgga atcagctcct tggtatctcc tccaagcctt
     9121 acagtctctt cgtgctgcgg atggccgtat cttggttgaa ggcttgtacg aagaagtaca
     9181 agagcccaat gaacgagaaa tggccttgct agaaacttat ggtcaacgaa acccagagga
     9241 agttagtcgg atttatggat tggagttgcc tctcttacag gaggagcgga tggcctttct
     9301 aaaacgtttc tttttcgagc cagcgcttaa tatcgaagga atccagtctg gttatcaagg
     9361 tcagggtgtt aagactattt tacctgcaga agccagtgcc aagctagagg ttcgtctggt
     9421 tccgggccta gaaccgcatg atgttctgga aaaaattcgg aaacagctag acaaaaatgg
     9481 ctttgataag gtagaattat actatacctt gggagagatg agctatcgaa gcgatatgag
     9541 cgcaccagcc attctcaatg tgatcgagtt ggccaagaaa ttctatccac agggcgtttc
     9601 agtcttgccg acgacagcgg ggacaggacc tatgcatacg gtctttgatg ccctagaggt
     9661 accaatggtt gcattcggtc taggaaatgc caatagccga gaccacggtg gagatgaaaa
     9721 tgtgcgaatc gctgattatt acacccatat cgaattagta gaggagctga ttagaagcta
     9781 tgagtagaga tattatcaag ttagatcaga tcgatgtgac ttttcaccaa aagaagagaa
     9841 ccatcacagc ggttaaggat gtgaccattc acatccaaga aggggatatc tacggaatcg
     9901 ttggatattc tggagcaggg aaatcaaccc ttgtacgggt gattaatctc ttgcaaaaac
     9961 catctgcagg gaaaattacc attgacgacg atgtgatttt tgatggtaag gtcaccttga
    10021 cggcagagca gttgcgtcgt aaacgtcaag atatcgggat gattttccag cattttaacc
    10081 tgatgagcca aaagacagca gaggagaatg tagcctttgc ccttaaacac tctggactca
    10141 gcaaggaaga aaagaaggct aaagtagcta agttgttgga cttggttggt ttggcagatc
    10201 gtgctgaaaa ctacccttca caactatctg gagggcaaaa acagcgtgtg gcaattgcgc
    10261 gtgccttggc caatgatcca aaaatcttga tttcagacga gtcaacttct gcccttgacc
    10321 ctaagacaac caagcagatt ttgaccttgt tacaagattt gaaccaaaaa ttaggattga
    10381 cagttgtctt gattacgcat gaaatgcaga ttgtcaaaga cattgccaac cgtgtggcgg
    10441 ttatgcagga tgggcatttg attgaagagg gaagtgtcct tgaaatcttc tcaaacccta
    10501 aacaaccttt gactcaagac tttatctcaa cagccacagg tattgacgaa gccatggtca
    10561 aaatcgagaa gcaagaaatc gtggaacact tgtctgaaaa cagtctcttg gtgcaactca
    10621 agtacgctgg agcttcaaca gacgagccac ttttgaatga attgtacaag cattaccaag
    10681 taatggctaa tattctctat gggaatatcg aaattctcga tggtactcct gttggagaat
    10741 tggtggtggt cttgtcaggt gaaaaagcag cgttggcagg tgcccaagaa gccattcgtc
    10801 aagcaggtgt acaactaaaa gtattgaagg gagtacagta agatggaatc attgattcaa
    10861 acctatttac caaatgtcta taaaatgggt tgggctggtc aggcaggctg gggaacggct
    10921 atctacttaa ctctttatat gacagttctt tccttcatta tcggaggctt cttggggcta
    10981 gtggcaggtc tctttctcgt cttgacagcg ccaggtggtg tcttggagaa taaagtcgta
    11041 ttctggattt tagacaaaat tacctcaatt tttcgtgcgg ttccctttat catcctcttg
    11101 gcaatcttgt caccactttc tcacttgatt gttaaaacaa gtatcgggcc aaatgcagcc
    11161 cttgtcccac tttcttttgc agtctttgcc ttctttgccc gtcaggtgca ggttgtcttg
    11221 gctgaattgg atggcggtgt cattgaggcg gctcaagcga gcggagcgac tttctgggac
    11281 atcgtgggtg tttacctatc agaaggtctt ccagatttga tccgtgtgac gactgtgacc
    11341 ttgatttccc ttgttgggga aacagctatg gccggtgcgg ttggagctgg tggtatcggt
    11401 aacgtagcca tcgcttatgg atttaaccgc tacaatcacg atgtgaccat cttggcaacc
    11461 atcattatca ttttgattat ctttgcaatc caattcttgg gagatttctt gactaagaaa
    11521 ttgagccata aataaaaaag agccgcgtgg ctctttttaa ctgatcagat tttctgtgca
    11581 aattttttac tcaaggcttg tccaatcaag gcacccacta gggctccgat gacaatactt
    11641 gcgataaata gaaggacagt tccagggttt ggagcgacca tgatgcggtc gatatattct
    11701 tgggattttc ctcttgccag aagagtagcc atataggctt tgggcgcaat ccacataagc
    11761 aagattggtc ctgttgtact aaaggcgaaa ataatgaaag aaaggaagtt ctttgttttg
    11821 tccttgtatt ttcctaaatg agctactcca tctgctagga ggccacagat aattccagga
    11881 aggaaggaac cagcaccgtg tttagttccc aagaaaaaga gggcaatgac aaggccgata
    11941 gtggtaatgg ctccaaagcg cggaactttt gcgactagga tcatatagac gctaccgccg
    12001 acaagggcag taaaggcagg cgcataaaac atgtttccag tttggtcaaa gagattgccc
    12061 aaaaggacac caatccccat gcagaggaag taaagaactg caaaaagcag tgtagttaag
    12121 atagatttct tcatgaattg tctcctgata attttttcac aattctcatt ttaccatggt
    12181 ttggtgggat tgtaaatgga tactagaagt ttttgaaaac gcttgaaata tgatataatg
    12241 gtttcatagt tatttttagg ggaatatcat gggtagattt ttagactttg tctttaatcg
    12301 tttcttttta gggatgattg cgacagcctt cttttggcta ttaactttag caggagggat
    12361 tatccttggt ctagcgccgg ctagtgccac cttgatgagc ttatatgcag aacatggtta
    12421 tagctttcgg gaatacagtt tgaaggaggc ttggtctctt tacaagcaaa attttgtctc
    12481 aagcaacctg attttctata gctttttagg tgtgggtcta gttttgacct atggtttgta
    12541 tctcttggtg caattgcctc atcagaccat tgttcatttg attgcgaccc ttttgaatgt
    12601 cctagtagtt gccctgatct ttttggctta tacagtatct ttaaaattac aagtttattt
    12661 tgccttgtcc tatcgaaata gtctcaaatt atccttgatt ggcatcttta tgagtctagc
    12721 agctgtggcc aaggttctcc ttgggactgt gctacttgta gcaattggtt actatatgcc
    12781 tgccctgcta ttttttgtag gaattgggat gtggcatttc tttatcagtg atatgttgga
    12841 acctgtctat gaaatcatcc atgaaaaatt ggcgacaaaa tagaatgaag cagttttggc
    12901 tacatacgct tctaagaacc tatagttcag tgatgatcat tatcattgcg agttttgcaa
    12961 tcttactctc ttacgctgac tgggattcac gtgaaaagga agcccagaga gtagcccagc
    13021 gtgtaactgc tagaacagtg agtgaaattg aatattacca tagagagtca acccagatag
    13081 ctcaggcttt agttgaaaac caagctcgta ttgagggaat ctataaatac tttagcctta
    13141 gcatgccaga ctatttttac tggcaattag agcggaaagc ttcgccttat atatcagtct
    13201 ctctgtatga aaatgttgat gacctctatg ttcgaaatga ttttgtaact ggggtggcca
    13261 ttgcttttca agattacaag gaagtctatg tttctactaa agacaaacgt agtggagaaa
    13321 aaatcagggc tgaggatttc aaaccagcag gaaatagttt tgccattcca gtgtcagatc
    13381 cagtgtcaga tcaagactta ggagtgattt acatctcctt ggatcctgct gttttatacc
    13441 atgccattga taatactaga ggtcatactc cgatggcagt aacagtgacc tcaccttttg
    13501 atacggagat ttttcatatg ggtgagacag ttgataagga gagtgaaaat tggctagttg
    13561 gcttaacttc tcatggttat caggttcagg tggcagttcc caaaaacttt gttttacaag
    13621 gaacggtgac cagctctgct ttgattgtgg gcttgagcct tctctttatt gtcattcttt
    13681 atctgacttt gaggcagacc tttgctaatt atcaaaagca ggtagtggat ttggtggatt
    13741 ccatccaagc tattgcccaa ggacaagaag gtcttcgcat tgatacgctt gaaaaggatc
    13801 aggaattgct cctaatcgcg gagacgacca atgatatgtt ggatcgattg gaaaagaata
    13861 tccatgatat ttaccagtta gaactcagtc aaaaagatgc caatatgcgg gccttgcagg
    13921 cgcaaatcaa ccctcacttc atgtataata cgctggagtt cttgcgtatg tacgcagtca
    13981 tgcagagtca agatgagttg gcagatatca tttatgaatt cagtagtctc ttgcgtaaca
    14041 atatttccga cgaaagagag accctcctca aacaggaatt agaattttgc cgtaaataca
    14101 gctatctctg catggttcgc tatcccaagt ccattgccta tggtttcaag atagatccag
    14161 agttagagaa tatgaagatt cccaagttta ccttgcaacc gctggtagaa aactatttcg
    14221 cgcatggtgt tgaccacagg cggacagata atgtgattag catcaaggct cttaaacagg
    14281 atgggtttgt ggaaattttg gtggtcgata atggtagagg aatgtcggct gaaaagttgg
    14341 caaatatccg agaaaaatta agtcagaaat attttgaaca ccaagccagc tacagtgatc
    14401 aaaggcagtc tatcgggatt gtcaatgtac acgagcgttt tgtgctctat tttggagacc
    14461 gctatgccat tactatagag tctgcagagc aagccggtgt tcagtatcgt attacaattc
    14521 aagatgagta gaaagggaga aaatgtataa agtattatta gtagatgatg agtacatggt
    14581 gacagaaggt ctgaagcgtt tgattccctt tgataagtgg gatatggagg tcgtcgcaac
    14641 agccagtcat gccgatgaag ctctagaata tgttcaggaa aatcctgtcg atgtcatcat
    14701 ttccgatgtc aatatgccag acaaaacagg gcttgatatg attcgggaga tgaaagagat
    14761 cttaccagat gctgcctata tcctgctctc aggttatcag gagtttgatt atgtaaaaag
    14821 agcaatgaac cttagtgtgg tggactattt ggtcaagcct gttgataagg tagagctggg
    14881 aaatctgctg gagaagattg caggtcagct cggcgagaga gggaagaaaa gtcagactct
    14941 tagtcaagaa ttagacgagg ctggatttgt tagttattta ggggataagg agaattggtg
    15001 gataggtcta tccaaggaaa aacaaggttc cttcaccatt ccctactatg tcttgggcca
    15061 agactggcag attttcattt ctgaccaacc cctagatggt ttagtcgtta caccttttga
    15121 agctccttat caagaacact ttgaacgctg gaagctgaat gctgagaaaa ccctctttta
    15181 cggttctgta aatttgcagc agtctgagag tctctttgcc tattacgaac cgatttatag
    15241 ggttattatt cagggaaatc tcaatcaaat cgtagaagag ttaaatctct tggagaaggt
    15301 agttcttgaa aatacgccgc gtgttccgat tactaaacag ctttttattc agtttgtcat
    15361 ggatgttttc catttatttg aacatctcaa agctgatgac ctgacagaca tcgtcaaaac
    15421 cattcatgct attcaatcct tcgatgaatt ggttccttat atcaaggaaa ctctgaccag
    15481 ctttttcggt caataccgta tgaatgaaaa tgtggtcagt gtgctggaag tcattggtcg
    15541 tgattaccaa aaagagcttt ccctcaagga tatcagtaag gctctcttta tcaatcctgt
    15601 ctatctaggg cagttgatta agcgtgaaac cgattcgact tttgcagagt tactaaacaa
    15661 acaacgtatt aaggctgccc aacaactttt gctttcaact agtgacagca tcgaagatat
    15721 ttgttatgct gttggttaca gtaaccttgg atatttctat aaagttttcc gaaaattgtg
    15781 cggaaaatcg ccaaaagcct accgaaaaca ggtagaaact atactataaa atttgtattc
    15841 ctttacaaaa ggtgctataa tattatcagg aggttctatc atgaaaaaga aaccaattta
    15901 tctatgggtc ttgctaatct tgtctgccct tatttcagtt ccgtctctgt ttggaattgt
    15961 gagtcccttg cctagtaaag aagcccttcg tgctgctcag aaacaagttg caggggtcaa
    16021 tgctcagcaa ttagaagacc agcttaatta tacctataga gtagcagaag catctcattc
    16081 tatttttaat gttgctttga ttgtgctatc tactatttta gtggtcgtag cgattgtctt
    16141 ccttgttcgt aaaaatttgc aatatgcaaa ctatacttac gttggctatg ttttgttagc
    16201 tattattggt tcgatttatg gctatgttgg tttacaagac gctgtgcagt tggttcaaga
    16261 tgagagcatg cgtttgacag tgagtattgg ttcaaaagct gttagcattt tctatatcgt
    16321 catcaatgtt ctcttcctag cccttgtctt ctataagatg tggcgacaac agaaagcctt
    16381 ggcagaagaa gaggaaacag aagagcttgc ctaagtattg acaaaaaaga actatcaaga
    16441 tacaatcttg gtagttgttt tttgattata gataaaatca tggaggtaca tatgaagaca
    16501 atttctttag tttatattag tttgagtggc aacactgaaa gttttgtgac gcgcttgaaa
    16561 gactatctct tgtcccagta caaagggatt gaggttcaaa agattcatat caaggattta
    16621 gtcaaggaag gcaaaaattt ctatgaaatg gaccatccct atgtcgcttt tttgccgacc
    16681 tatctcgaag gtgggaatgg cgtggataac ggagatgttg agattttgac gacaccggtg
    16741 ggagatttta ttgcctatgg taacaatgct agtaagtgtt ttggtgtggt tgggtcagga
    16801 aatcgtaact ttaataacca atactgccta acagccaagc aatacagtca acgctttggc
    16861 ttccctgtat tggctgactt tgaaatgcga ggcatgctgg aagatatcaa acatgtcgca
    16921 gctattatcg cagatttgta tgagttggaa aaggagaatt aatactcaat gaaaatcaaa
    16981 gagcaaacta ggaagctagt cgcaggttgc tcaaagcaca gctttgaggt tgcagataga
    17041 gctgacgtgg tttgaagaga ttctcgaata gtataaagtt cttataagag gtggttgaag
    17101 ttgttcaact gttttttgag tataaacagt ctttaagaac gtaaaaaagc ataagttcag
    17161 gtcttcaaaa ccttgttctt atgcttttta tcatagaaat atttgctgaa ttttctcttg
    17221 aaatgaaacc ttatcttcat ctcgcgcttg tcccttaccc tgcttttccg attgcgagtg
    17281 cgtagataaa gaagatggcg aagccaaaga ggaaaatcaa tcccgcaatg gcaaggtgtt
    17341 tgagccaaga aggaagattt ttgttttcac gcgttaccaa ggcgtaattc aaaagagcaa
    17401 agaaaggtgt tgtcaggaaa gagccaatca tggcaaagcg gagcatggtt gaaacctgac
    17461 cagcgaagaa cttgataatg acgataccga tgatagcagt gatggtcatc cagatgttca
    17521 aagatttacg attgtcctct ttttgactga ttagcagtcg gagagattcc tgattaacgc
    17581 gagaatagcc atcgataaca gttataactg ttccaaagat acagaggaag gcaataaagg
    17641 taatcaagta acgggaccat tcgccaagaa cagaggcata catacccacg aattgagaga
    17701 tgtatttggc tgaagcagct tcaaccgcct gccctgtagg atactgaatc agtgctccca
    17761 gtgccacaaa gaagacggct aggatagctg ttccaatata accagtgtta aagtcaaaca
    17821 gagcgtcttc tgtgttaaag ttgacggtct ttctcttttc agctgaccaa agtgaattga
    17881 tggctgaaat ttcaatagga gccggcatcc atcctaggag ggagacgatg aagggcagag
    17941 ctgccatttg ccaaggtgtc ttctcgacaa aatcagaact gtattctgga tgcttgaccg
    18001 ccgcaatgat aactgcaaga acagtcgcaa tggttaaggc agacataatc catttgacca
    18061 tgccgtctaa aagtttgtag cctccaaaga gtagcatagc ccaaatgatt gcaacgagaa
    18121 tgagggacca ctgagtaatg ctaagtccaa tcattgggaa ggcactggcg atgatagctg
    18181 agcacagaat ggcaacacca gccgtgttga ccatagccga aaagacattg aggataaaga
    18241 aaatccagag atagagtttt cctttttcgg cataaccttc aaccaaagtc tttccagtat
    18301 cagctgtgta ttcagcacca aaacggaaaa atggatattt aaagacattg gctaagatga
    18361 ccaagagaag tagagaccaa ccgtaagaac cgccagcttg agttgaggat acaatgtggg
    18421 aacctccaac agcggcagtt gccattagga ttccaggtcc cattgctttt agcttgcttg
    18481 cccaggttga ttgatttaag gaaatagctt gcgacatgat aaatactcct ttttgaattt
    18541 tcagaataat ctgtatatgt aatctatatt ctacaaaaaa ttttggaaaa atgcaagagt
    18601 attttgaaaa aagatagaaa atttacaaag aagatctgaa ataactgtta tgatatagag
    18661 gattgaaact agaatagtac acctctgctt ctaaaacatt gttagaaatc gatttgactg
    18721 tcctgatcga tttatcctgt tcttatttca tttcactata gtaaggtgca gcaggataaa
    18781 gaaaaaccga gaagtttctt tctcggattt gcttattatg aggttcctat tttctatgct
    18841 ccaagagttg attgatgtga tctacttttt tagcctctct tttatagaga atagcccaga
    18901 tgatgagata gacaatcgca aactcgataa tgagctggag gtagaaaatc cagtggaaag
    18961 ggaaccaacc agcaagagtt gctagtggga caaagccagc cagcataagg aagaaatgag
    19021 tcagagtggc acgaagcatg ctccagtcac ggctaaataa gcggttgcca aagttgaaga
    19081 gcataccgat ggttgcccag ataagtgtgc agtagagcaa gaccagggca ccgtgaacct
    19141 gatgctgatc catcacttgg ccgatgagag agtagggatt tagtggtgcg taggtatttg
    19201 gtgcataaat gagtgaaaag aggatagaga ggatgaggcc gataagaaca ccggtagctg
    19261 catcgtgaaa tacttgtttt ttcatagttc taatttctcc ttgatggttt ttagataacg
    19321 gcgtgaagag taggtgaagc tttcgttttt caagaaaatt tctaccagac cgtttggcgt
    19381 gagcttgagg tgagagatgg aatcgatatt gatgatttct gattgggaaa tttggataaa
    19441 attggttggc aagagtttaa gaacctgata gagtcgcaaa tcaatgctgt aggtctgact
    19501 cgcggtttct gctagaacct tccgattctc gatatagaag cgctgaatct tgccaatctc
    19561 aactagatag acctgatcat cgatttttcc tttgattttt tctctttggt ccagattttc
    19621 tgcgaactcg atgactttct ggactttttc ggtttcttga ggtgcttgga caatcagctt
    19681 ttcctcctcg taagtctcac taatctgtag ttctactttc atagttttct ctccttttca
    19741 gttatacaag gttgtgatca cttcctgtat atccttatta aacactattt tatagcccat
    19801 ggcaaggaac cttgtctatg tggagggatt gggctcctat gtggtggagc ttttctgttc
    19861 tttctgaaat atggtataat agcactaatc aatttctagg aaaatagata cggaaagggg
    19921 ctgaaagaac taggggaaca gttggaatta gaaagtgata ggtatgaaaa gtggcgttga
    19981 agctgaataa gtttataaag cgtttgaatg aagaaatgga aagtaaataa gttgacgatg
    20041 aattacaaaa tagattttat ctacaatttg tatcttttaa atcattgatt tgaattttag
    20101 atttaatcat gttacaatta aaattgaaat caaatagaag aggaaaatgc aaacgtgggc
    20161 acactattag caacaagatt aaaaaataga cggaaagaat taaaaatgtc tcagcgagaa
    20221 ctagctgaag gtatctgtaa acaggggcaa atcagtcgat tagagagcgg agagtttacc
    20281 cctggagcgg atttcctgaa tgcccttgct aagaagttaa aagtcagtat ggattatttt
    20341 tttgatgagc aggttgtaga gaaagttgag gagttatcag aatttaagaa attagcacag
    20401 acatttatta caaatcgcaa ttatgagtct ttgaaatata tatatgaatt ggagaatgta
    20461 aaagctcacc gcctatcttt agcagataaa atctacatgg aatggataaa atctctgata
    20521 gatttttatt tttacggaca gaaagaagat gcgattgtac gattagaggg ggtattatca
    20581 cagttaagtg tatctgattt gaactatctc caagtttcaa acactctatt caatttttat
    20641 tatgatattg gagatttagg tcgtttcaat aaaattcgtg aaaaactaga gtatcaagta
    20701 aatcacctca atttgagtac aatcgaagag ttagaactat ctattaagtt taactacaac
    20761 gtttgtcgtt atctatggtt acagaaaaat attgaagaag ctattactaa aattacagct
    20821 actataaaac aatgtaaggc ttacagaacg aattatcttt tggctgattt gtacttattg
    20881 atgggaaatg taagtgaaaa tttttcttct aaaagttcgg tgaaagaata ttttgagacg
    20941 gcatattttc tatacaaact tgaggagaac atgtcaatga ctctgaaagt tgagcattac
    21001 attgcagata taacagaata atgaatacgg tgttcaagtg agcatcgttt ttcaaaccta
    21061 gttcagatac aaaaaagtat caaacaaaaa aaaataaaaa aacattataa aaacaattga
    21121 cgagctaatt ttgaagtgat aaaataaatg caagaaaaca aaaggagagg tatatgagaa
    21181 gtttgtttaa gaaaatagtt gctgtattag ttattggctt aattctgtta ggaattgcga
    21241 gaacacctca agttcataag atggctagag ggattgatcc tggtcctgcg aattttattt
    21301 agaaatcaat ttctactaat tgagtattaa tgggtaaaaa gattgtatgg ataagaaaaa
    21361 tttacttgta ttggttactg g
//
LOCUS       DAVKHZ010000030        21364 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000030 DAVKHZ010000000
VERSION     DAVKHZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.030"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(437..2164)
                     /gene="ezrA"
                     /locus_tag="VWW99_001047"
     CDS             complement(437..2164)
                     /gene="ezrA"
                     /locus_tag="VWW99_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9538902.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2246..4192)
                     /gene="gyrB"
                     /locus_tag="VWW99_001048"
     CDS             complement(2246..4192)
                     /gene="gyrB"
                     /locus_tag="VWW99_001048"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9538903.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4207..4770)
                     /locus_tag="VWW99_001049"
     CDS             complement(4207..4770)
                     /locus_tag="VWW99_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9538904.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4855..5409)
                     /locus_tag="VWW99_001050"
     CDS             complement(4855..5409)
                     /locus_tag="VWW99_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9538905.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5420..6643)
                     /locus_tag="VWW99_001051"
     CDS             complement(5420..6643)
                     /locus_tag="VWW99_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9538906.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6680..9130)
                     /locus_tag="VWW99_001052"
     CDS             complement(6680..9130)
                     /locus_tag="VWW99_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9538907.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9330..10259)
                     /locus_tag="VWW99_001053"
     CDS             complement(9330..10259)
                     /locus_tag="VWW99_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9538908.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVHKLHPKNRFLDTGFYDYDPDMVR"
     gene            10399..10686
                     /locus_tag="VWW99_001054"
     CDS             10399..10686
                     /locus_tag="VWW99_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9538909.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10720..12054)
                     /gene="ciaH"
                     /locus_tag="VWW99_001055"
     CDS             complement(10720..12054)
                     /gene="ciaH"
                     /locus_tag="VWW99_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9538910.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12044..12718)
                     /gene="ciaR"
                     /locus_tag="VWW99_001056"
     CDS             complement(12044..12718)
                     /gene="ciaR"
                     /locus_tag="VWW99_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9538911.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12827..15373)
                     /locus_tag="VWW99_001057"
     CDS             complement(12827..15373)
                     /locus_tag="VWW99_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9538912.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15496..15936)
                     /locus_tag="VWW99_001058"
     CDS             complement(15496..15936)
                     /locus_tag="VWW99_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9538913.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCVVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15911..16780)
                     /locus_tag="VWW99_001059"
     CDS             complement(15911..16780)
                     /locus_tag="VWW99_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9538914.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAESHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGVKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16822..17550)
                     /locus_tag="VWW99_001060"
     CDS             complement(16822..17550)
                     /locus_tag="VWW99_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9538915.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17558..18037)
                     /locus_tag="VWW99_001061"
     CDS             complement(17558..18037)
                     /locus_tag="VWW99_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9538916.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKARGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(18048..18746)
                     /locus_tag="VWW99_001062"
     CDS             complement(18048..18746)
                     /locus_tag="VWW99_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9538917.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18739..18975)
                     /locus_tag="VWW99_001063"
     CDS             complement(18739..18975)
                     /locus_tag="VWW99_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9538918.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(19075..20001)
                     /locus_tag="VWW99_001064"
     CDS             complement(19075..20001)
                     /locus_tag="VWW99_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9538919.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20076..20891)
                     /locus_tag="VWW99_001065"
     CDS             complement(20076..20891)
                     /locus_tag="VWW99_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538920.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20895..21215)
                     /locus_tag="VWW99_001066"
     CDS             complement(20895..21215)
                     /locus_tag="VWW99_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9538921.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6459 a         4676 c         3754 g         6475 t
ORIGIN      
        1 aaacaaagag gttttagagg cctatttacc gtagactaaa gttgtacaag aaaagtgcaa
       61 ataagaaatc tccagattag gaactatccg tgagttctct agtctggaga tttttcaata
      121 gacttcatta ttggacggtt accaagttac tcaaagtaca gatttgaagc tgcagataag
      181 actgacgaaa tcactttaat actaaatgaa aatcaaaaag caaactaaga agctagcctc
      241 aggctgtact tgagtacggt aaggcgacgc tgacgtgatt tgaagagatt ttcgaagagt
      301 ataaaacact gttttgaggt ggtggataaa tcggctgggc aaaaactcaa tctcaggaga
      361 aaatgaagta aatctcccca caactataga aaaagatttg attctgctcc tcacacaata
      421 aaatcttttt cttttattaa aaacgaatcg tttcacgtgt tttctcatat gaggtaacaa
      481 agcgattggt tacaccaggc tctgccactt ccaatgcttg agaaatcttg tcaaatgaag
      541 cgtgataatc aaattctttt tcaaaaatat ctaaagcttc gttaaatgct tcttgaatgc
      601 gttcatcaaa tgagcgatag cggttagaat attgcaagag ttgctctgtc aaagttgcat
      661 attgtacaat attataagtt tccgtttcta aagcttccat atcattcgtt gcaatttcaa
      721 gaactcgggt aacagattca atgttaatca ttttttgttc taactcaacc attaaatcct
      781 cggtattatt gcttgccgta aagaataact tcaagaaagt ttgtggaata cctggcagat
      841 tgcgtttttc catgtatcgc ttgatagtat ggagacgatt gacataaaca ttggcctttt
      901 gacgtgcatt aatatcatct ttctcaattt gtgtcaggcg ctcactaact gaaatttgct
      961 catcttcaat atcttttagt tgagtttgta aatcctcaag attttcttca agaactgaat
     1021 aagcttgggt tggttcttct tgatttgaag ttacctcaac aatagctgcc tcaaaactct
     1081 ctaattctgt ctgaatacga cgaacatggc ttgcagctgt ctcaggaagt aaataggtct
     1141 tgttcaaacg tgcaatatct tctcccaata aagtattatt ctctttcata tgttgaaggt
     1201 aagttggaag agttgcaagt agattttcca ctactttctg agcagcaatt tctcgagtaa
     1261 aaatatcata caaggcattg atttcctctt gtgcctgtcc attctcatat tcggcattat
     1321 ccaattccaa ctgacgaata ttctcttggt ttttcttgaa tgcttcataa agcaagtgga
     1381 aacgcgcttc aatatccgtt tcaacaaaat gataattagc atcaattagt ttacgataac
     1441 cggcttccaa atcctgtaat tgatctggca attctgtaga aagcgtcgta accaaggctg
     1501 gaacacgatc cacaatatga cttaaggcca aaatgtgatt ttctgtatta tccaaaatca
     1561 ctgcggcttc cacagggtca cccgatgaat tcaaggttac aaattgtgaa aattcagatt
     1621 ggatattttc taattgtttt tcaatttcat ccaaggcttg accatactgt tctgaatttt
     1681 cagcaactct atgctgaagt tcctcaaata aatccaaagc atgaagaaca cgaccactat
     1741 ttttagattc ttgcttctct aagtctgcca aagcattgcg aattgccgca atatcttctt
     1801 caatcaaagt aatttgactc tcaatttggt caatttgatg actggccttg agaaaacgaa
     1861 atgaatggtt atagccttct gcttcaaaga gattattttc aatatcggca aaagagttga
     1921 gagataaatc gacccatttt tgattccatt cacggaaagc cacttgactt tgtccaatca
     1981 agtgcatatt ttttacagct tctacttcat catttactgg aagattgtat agttcttctt
     2041 ttctttcttc tagcgcctct aatctcccct cgtttcgctt acgtagaaag attgccacta
     2101 cataagccag aactaaaatg actgcaattg caaccattaa ataaattagt tgtccattag
     2161 acatatcaaa ctcctttttt acttgaaaca atcgtaacga ttatatcata ttttttagac
     2221 caagggaact acttctccct attttttaga catcaagtgt actatagaca gcattttctt
     2281 cgataaactc acgacgaggc tctactcgat cccccatcaa catatcaaag attttatctg
     2341 cttctgcagc atcatctaca gaaactctag ccatcaagcg atgttcggga tccatggttg
     2401 tttcccacag ctgatgatcg tccatttcac ctagcccctt ataacgctga atagtcggtt
     2461 tggtacgacc ttcactataa cgggctaaag cttcttggag tttgatttct tgatctgcac
     2521 ccggctggat atattcttta atctcgcttc caaccttgac accatagatt ggtggttggg
     2581 caatataaac atagccagct tctaggattg gtttcatata acgataaatc aaggttaaaa
     2641 gaagggtacg aatgtgggct ccatcgacat cggcatcggt catcaaaacg agtttttggt
     2701 aacgggcttt cgaaacatca aattctgcgc caaatcctgt tcccatggct gtgaaaagac
     2761 tacgaatttc ttcgttagct agaatcttat ccatacttgc tttttcaacg ttcaaaatct
     2821 taccgcgaat tggaaggata gcctgaaact cacggttacg accagatttg gctgatccac
     2881 cagctgagtc tccttcgacg atgaagagtt ctgtttcagc agggttatta gaagaacagt
     2941 ctgctagttt ccctggaagg ttggaaattt ccaaaccaga ttttttacgt gtgacttcac
     3001 gcgcacgctt ggcagccaca cgagccttgg cagccaaaat tcctttttct acgatacgtt
     3061 tggcaatctg tggattttcc atgaggaaat cggagaaggc ttcactgaag aggcgattgg
     3121 taatcttgac cacttcgcta tttcccaatt tggtcttggt ttgtccttca aactgtggat
     3181 ttgggtgttt aactgagata actgcagtta agccttcgcg aacatcttcc cctgttagat
     3241 tgtcttcatt gtctttcagt aacttatttt tacgagcata atcgttgata acacgtgtca
     3301 aggctgtacg gaaaccttgt tcatgcgttc caccttcatg tgtatgaata ttattggcga
     3361 aactcatgac attttcatgg taacccgttg tgtactgcat ggctacctca actgtgatat
     3421 catccatctc accgtctgta tagattggtg tatcaaagat tacatccttg ttctcgttga
     3481 tatattcaac gtaactagca atcccacctt cataatgata atgcttggtt tgttccaaac
     3541 cttggcgctt atcagtgata gaaatttgaa gaccgcgatt tagaaaggcc aactcttgaa
     3601 tccgtttatt taatttatca aaatcaaaga ttgttgtttc agtgaagatt tttgggtccg
     3661 gtgtgaagtg aacagttgtt cctgttttat ccgtatctcc aactatttca agatctgcga
     3721 caacatgacc acgacggtat tcttggtaat gaatcttacc gtttttatgg acatgaacgt
     3781 ctaattgagt ggaaagggca ttaacaactg acgaccccac cccgtgaaga ccacctgaaa
     3841 ccttgtatcc accaccgccg aactttcctc cagcgtgaag gactgtaaag acggtctcaa
     3901 cagcaggacg acctgttttt tcctgaatat cgactgggat accacgccca tcatccacaa
     3961 cagtaatcga atcatctggc tcaataaaaa cttgaatatg gctggcaaat cctgccaagg
     4021 cctcgtcaat tgagttatca acaatttccc agactagatg gtgaagacct tcttttgagg
     4081 ttgatccaat gtacatccct ggacgcatac gaacagcctc taagccctct aaaacttgaa
     4141 tttgactggc atcataatcc tgtgcctgca gatttttgat ttcttctgtc atcttattcc
     4201 tttttcttat atgtctaata cttgtcttaa attcacgata ctggtaaaca agtgggtatc
     4261 aagtcctgca gcttgacctg cttcgatatc aatcggccga tcaccaatga caagaccaga
     4321 gctaatctga tacttttctc ttaaataaag catggattcg ggatttggct ttctcttaaa
     4381 gcctgagcta gaagtcacca cttctgtaaa ataagctgct atagaggttt tttctaaaat
     4441 ttccaaaacc tgatcatttc gatgagagac caaaaaatga cggccacctt gatttaaaat
     4501 gtcttccaat aggtcagaaa ctccttcaaa taaaatcggg tgttcaagct ctctggcttc
     4561 attttccttg tacttttcta aaaaattctc taaattggga gcgaatgtct caatcgcaaa
     4621 aggagtagaa acctttaaag cttgatagac actgtcatgg tcttgtgtga taccatacag
     4681 tgccaatgtt tcaacaaatg cagctgttga agtttcataa ttatccagta aagttccacc
     4741 taaatcccag atgtaatcgt gatatttcat acccttcatt atagcatttt tttatgaaaa
     4801 aagcgatgat ttccctgagt tttccacata aaaaacgaga gaataactcc cgttttactg
     4861 atttttacca aagacatctc gatagagcat tcctgttcgt aaactctctg cgtcccctcc
     4921 tagttgctcc accaactcgt aggcaaaggc aagggctgtt gaaggacccc gactggttgt
     4981 caactgacca tctaccacta ctgtttcctt gacgtagtga ccatcaagga tttgctcttg
     5041 aacgccgtca taacaagtgt atcgcttatt tttcaatatc tctgcttgat tgagggcaat
     5101 tggtgccgca caaatggctg ctagtttctt cccttcttgc tcgaagcttt gcaattcttg
     5161 aatcaaggtc tgattatcac gtaaatgtgc agaaccaggc atacctccag gaagaacaat
     5221 catatcatag tctgataaat ctccatcaaa gacatgatct gctcttactt ggattgcatg
     5281 cgaacccgtt acttgctctt caaaaccaac catatcacat gtgatattgg ctcgacgcaa
     5341 gacatctaca actgtcaagg cttcaatttc ttcaaagccc tgagctaaca taactgctac
     5401 ttttaccatg attttctcct tatttaattt gttttaatac aacctttttc cgtttgaatg
     5461 ggacttttcc gctcttttct tcagctagat tagtctggta actcatcgat aaaagcaaac
     5521 caacaccaat cagattactg ataatagctg atcccccttg cgaaatgaaa ggcaagggaa
     5581 tccccgtcaa aggaagtagt ccagtcacag caccgatatt ctcaaagatg tggaagagca
     5641 acatcataat caaacctgtg gaaatataag tgtagaactg gttatttgat ttaagagtaa
     5701 tcttcaacat acggtaaatc aacatgagat agagggcaat aaccaggaca gagccaataa
     5761 agccaaaatc ttctgcaata accgtaaaaa tcatatctga ctctcgaact gggataagca
     5821 gattcgaagc attaaatccc tgaccaaata agccaccact cccaatggca atctgccctt
     5881 gagcctgctg gtaagtcgtt gtttgggcaa actcaaaggg attgagccaa gccaaaatcc
     5941 gattgatttg gtaggtcggc attccaatct ggtgaagaaa agctcgtccg tccttgctaa
     6001 taaagatagc taagaaacca gcaactcctg ttacagcagt cacaaatact gggataataa
     6061 ttttccaaga aacccctgat aataaaacga ttcctgagaa aatggctaca aaaaccaaag
     6121 ccgtccccaa gtcactttga agtgctaaaa gaactaggac tggaatggta aagagaatca
     6181 tccagaaaat taacaaaaag tccagcgaaa ccgtgcgtct ccattcctta tgtttctttg
     6241 taaattggac aatgacacga gccaacatga ggatatagga tatcttcata aattctgacg
     6301 gttgaaatag ggtaattcca tttattgata cccagttttt ggcacccgtt gatgcaacta
     6361 agcttggatt ataaaataca atcggcaaga tcataagtcc caagcctaaa atatatagaa
     6421 agggggtcac cttccaaaga aattctgtat taaagagcat gaccacaaaa ccaatcacaa
     6481 gccccaaggc aatccaggcg acctgctgcc ctaaaatggg cagaatatta ttgggataat
     6541 catgactaac ggctatatag atagccacca caccgatgac cagtagaaaa aatactggca
     6601 agagcaaact gtaatcgact cttgagtcga gagaacgttt catatatact aaccttatac
     6661 tttcatacaa tactatttat caaagttcat taaaaaatct atcaatagcc tcgtcaactt
     6721 cggatcgaga gatggtttta acagtcgctt cttctgctag agatgctact atttgtttgc
     6781 cgtatcgttt tccgacgatt ctcctatcca aaataagagt taaggaacgt tggtattcac
     6841 gtctcatact tcttcccaaa gcctgtttta aacgaataat ggccattggc aattgataat
     6901 cataaaaggc atttttccct tcttgattca gttcttgatt aatctttttc gtcaagggtt
     6961 cttgaggatt ttggaaagga agcctcggta caacttgaat cacagaagga tggcttgaaa
     7021 aatcaactcc ctcccagaaa cttgctgcac caagcaagat ttgttgttca cctttttcaa
     7081 agcgtttctt tagctgatga acatccccat ttttatactg ggccaagtgg ctaactgtaa
     7141 gtaaatccga tactgctaga agcatgtctt tagcggtaaa gagaaccaaa atcggttgct
     7201 ggaaagcttg aatttccact agtaaagcag ctacctctct ggcatagact tctaaggagg
     7261 tttctgttac cagagggaaa tctttgacca tgaccacttc ttgttcctgt tttccccgag
     7321 attcaatctt gacaaattta gcttcaggat agcctaaaag gtctgccaaa gaaaccctct
     7381 gactaatctc aagagtagcc gatactccca agacttggca actctcagga agtaaagagg
     7441 acagaagagt acggcctgat tttgtagaag aaatctgaat tttcttttga ctcgtttcag
     7501 ttacttcaag ccagtaatca ccttcagctg taaaatagcg aaccagctca tcaaagtctt
     7561 ctacttccaa ttctgaaaaa tactgatgga gattgtccag agaatctaag atatttttcc
     7621 tagatttgcc agactgaaat tgttctatca agtagagaca ctcaaagcga atactttcta
     7681 gtatccgttg ttgaaccctg ttttcttctc ctactaaagc cttatcaatt aaatcgataa
     7741 tagattgtat atcgtaggtc tcttgaagca gattttctag agctaacaaa atcttttgga
     7801 cttcatcaat aatcagtaaa cggtcactga caaattcagg attatcttca agtctggtta
     7861 cgagataggc atgattagtc actaaaagct tgcaagtctc tgccctttct tgactacgtt
     7921 tccaaaaatc ttccgtcaca aataagctct gggatgataa attcccatca tgacgaaggt
     7981 ctgctagaaa atgttggtaa cggtagagtt gcccgatttc atccaaatct cctgtctctg
     8041 tctcagtaag ccagaccaag acttgcattt taaagcgtct aaataagcga ttttcatcat
     8101 tttcctgcaa ggaatgataa aaggcatcca acttcagata attttgtggt ccctttaagc
     8161 tatgaatatc tgtatggaac acttccttga ggcgtttacc ttcttcttcc atgatttgat
     8221 tttgaagaat ctttgtcgga acactaagaa caatttgtcg ctctttggat tgagataaag
     8281 cgggtaagag atagccatag gttttcccaa tccctgtcgg cgcttgaatc agagagacag
     8341 gttcatcttt caatagcaag ccaacctctt tagcaaaact ttcttgttcc tccctcactt
     8401 caaggttcaa cagagaaata tttttagaaa agtcttgaga tagttttcgt agctccagag
     8461 aagctgccgt tttcttaaaa tacagacctt gaacttggac caagtctgga gaactcagga
     8521 tagattggtt gcgataagtt tcctcaataa ccaggtagga ctcatatagg agagcgtcag
     8581 ccatttccag caagcgttcc aagagacctt taggaagctg ggtcatcttt tcccgtaaaa
     8641 aaagaagtaa ttctgctgta gcttgggcat ctgaaagggc tgtgtgtgcg tgtttaagag
     8701 gaattcctaa ttctcgacac aaaatcggca agctatattt ttccagttca gggaaaaaga
     8761 cctgggccaa ttcgaccgta tcaacacgag ggtttcttag ctcatagcct tcaaaaaata
     8821 aattttccgc caagagatta gcatcaaact gaacattatg ggctacaaaa atcccatcct
     8881 ccaccaagtc aaatattttt ctggcaactt gcgaaaaatc aggtgcttgc gccagacgtt
     8941 ggtctgtcaa tcctgtcagt tctttgatat gagcatccaa gggttcatgt ggattgacat
     9001 ccgtcgtata gtgatcgacg atttctccat cctcaatcac gacaattccc acttggataa
     9061 ttttagcctt actacctgtg ctagttgcct ctaaatccac cacaacatag cgattaccaa
     9121 tcgttttcat tataaaaaat tataccaaaa aaagggccat ttggcacctg caaacatagg
     9181 ataattttca aactcaagaa cactactttt cttaaaaatc ccatcgaata gagcattcta
     9241 gcctgctgat ttttgtaaaa tagtgaagaa atacgaaaaa ttgagagaag aaatgtttgc
     9301 atcttccttc tctcaacgaa tcatgtattt tatctaacca tgtcaggatc gtagtcataa
     9361 aatcctgtat caaggaaacg gtttttaggg tgaagtttat gaacttcttc atccagcaag
     9421 aaggcttgat catggctaaa gttgccctct tgaatcaaat tacgcgcttg gataaagagt
     9481 tttgcaatct caacaaagtt ctgaccagga gtgtagagcc cttctagttt ccagcgagta
     9541 aagccatgct ccaccaattc tgttaatttg atcatcaaat caaggtcatt gttggcaaag
     9601 atatgggtcc catgattatc ttcaaaaatg gaatagtggc tctctggatc acttggctca
     9661 gccaagaaga ggtcacgttt atgcgtcttt tcatcatcga tatgtgtaaa gttatagtag
     9721 ttttgcaaga gtggacgttt agaatgatgg atgacgctag caccgtaaac caaaacttca
     9781 gcaggaattt ccaaaatctc tggcattttg aaaagttcag ctgatggaat ttcacgcgcc
     9841 aaaacagcct cagatgcgcc agccttttgt ccccagaagt taatctgacg actgctagtt
     9901 accatggttg aagcatcgta gatggtctta aatgaataac catcgcggtt aactacgtaa
     9961 aagacgcctg catccccaat cgtaatatag tctgtcttga tttcttccaa gaagtctaag
    10021 aaaggcttga tacggtccat catatcttgg tgcatgagag cattgaccgc aacgatcaat
    10081 tccttaccag catcatgaac caacttagcg atttcacgta attggtcata actaaaggtc
    10141 gttggcagac gaagaccaaa atctttctca ccgacataga tacggtctac gccagcttcg
    10201 agtagttgtt caacttgttc aatactttca gcagttgctg taatgacaat cttttccata
    10261 ggaaaaatta taccacattt ctccaaaatc agccatgctt tgaaaatgaa aagggacttt
    10321 attctttttg taacctttct gtaataattc tctgccgaaa aaatgataaa ataggtatgt
    10381 actgttaagg agagaatcat gcccgtaaga aaattacaat cctatgaggt agactatcaa
    10441 gaagaattaa accagcagct tcctcattac caagcttata cacctgaagc gcaatctgat
    10501 gccaatctca aggaaatcct attttttatt aatatcgctg ttttttgtat ctgtattgct
    10561 atctttagtt ttatcttttt agcattaaaa ttatcaactg ctcttgcctt tgccgcagca
    10621 atcggattca gcttacttgt tttaaaagtt caacggtcta ttatcaaacg aaaacgtaga
    10681 agataaatcc aaaatatcgc cccaattgga gcgatatttt tattttttct ttttagatgg
    10741 tgtctgaatg gcaatcttca cttcaaagat tgttcccttg ggtttattat ctttgacagt
    10801 aacagttcct tttagagcat ctacaatttg cttggctagg gataatccta aaccaaaacc
    10861 acctttttgc cgggttctag ccttgtctac tcgataaaaa cggtcaaaaa ttttcttttt
    10921 atcttctgtc gaaataccga ttccattatc agaaacaagt aaataaagat tgcgatcggt
    10981 cgccgagata agaaaatcaa tttcaccatc ctcctcagta tacttgacgg cattatcgaa
    11041 aagaatggtc atcagttgtt tcagaagaag ctgatctgtg acaattgttc gatggatacg
    11101 attttcaaaa cggaagacac gattattttc cgaagcaatc atctcatagt ttgtgaaagt
    11161 tgtattaaaa aagctagttg gaacttctgc aagctccggc ttaatcccat catctctccg
    11221 agctaagttc agcaagcttg tcgttaaaaa acgcatattt cggacttctt ccaaactcga
    11281 tgcaatgctt tcgctcacat ccataatggt agcttctggc ttacgaaaaa gggtctctaa
    11341 gcgattttgc aaaactgcga gtggagttcg taactcatga ctggcatttt ccacaaaaga
    11401 ctgttgcttc tgcatactct caagcagggg cctaacactg accctagcta gatagagact
    11461 ggcaagtaaa gacaaaatcc agaaactagc catcacgacc acaatcaatt gctcatgctt
    11521 ttgactagcc tgctccaact gactggtatt aatcaagaca gcagcatact tgatattggt
    11581 tgaaaccgaa ctaatattgg tctccatcaa aatcacacga tagatttctt cctgcccata
    11641 gctattaaaa acctgaatct ggtagatatg tcctagttct ttcttttcta acttaatctt
    11701 atccaagccc aaaaatcgat ttccagaaag aagttgagta aagtctttat caaagagaat
    11761 gacttccgta ttggaactga cattaggttt tatttctact ttactagcgt ccgccctagc
    11821 attttctaaa tctttaatct cttctgttgc cctatttata gccagctgaa taactgcttg
    11881 aggattttca ctcagtccat gaagcttatc gtccaccgaa gtataaagac tcgaatgcat
    11941 gacttgtaaa ataatcagag tcattgtaga aaaaatcagg gtgaagacac cgaagttgcg
    12001 gataaaataa ctaaagtcat ccgcatacca tgttttttta agtttactga acatctttta
    12061 aaagataccc aacactacgc aaagtttgca aattctctgc aaaagtggtt ccctttaatt
    12121 tcttacggac ttttgaaaca tagacttcga caaccgaaat cgttgtatca ctatcaaatc
    12181 cccatagacg gtcaaaaatc tgcgtcttag gcaaaatcac attttgattt tgaaggaaat
    12241 aaactagtaa gtcgaactct ttccccagca attcgacagg agtatcttca actttaacgg
    12301 tattggttga taaattaacc acgatattcc cataagtcaa ggtgttttca ttaaacttcc
    12361 ctgaacgttt gagaagggcc tgaatccgca ttttaagttc ttctaggtag aaaggtttgg
    12421 tcagataatc atccgctccc agttcaaatc catgtccctt gtcatccaaa ctttccttgg
    12481 cagtcataat cagaactggt gtcgtaattc ccttttcacg caattctttt aagacttgga
    12541 aaccattttt ttctggcaac atcaaatcca gcaaaatcaa gtcatagaca ccactctcag
    12601 cttcgtagag accttcttct ccatcaaata cctgcataac atccgcaaaa tcgtctaaaa
    12661 agtcaaatac tgaatttgac agacctaggt catcctcaac caataagatt tttatcatga
    12721 gaaactcctc cttattaaaa ctattatacc aaatttgcct taaaaaaaac tcaactctct
    12781 gcattttaca tgagatagct gagttttctt tttattttag gcttatttat gcatttccgt
    12841 attgaagaac aactgcttcg actgcagctt tttcacggct aatcaagtca acacgtgctg
    12901 caatttcctt gattcccata ccgatgttac ggctaagagc aaggtcagaa agttgcggct
    12961 caaagaactc cttgtattcc gccaagcgtt gctgagtctt aaatacatga gcaggaagga
    13021 taacaaagct atcaaagctc atatctcctc caagggctgc cttaatccaa gcccagtttt
    13081 cacgcgccca agaccaagct gttttctgag ttgcttgatg agctaggaat tggtaatacc
    13141 aagcagacaa gtcctgtggt ttgaccacaa atttgtcctt ccaagaagta atcaggtttt
    13201 ggatattatc cgcatctgta ctgtatgcaa gagctgctgc caactggcgt ttaaagacag
    13261 catctgttgc gtgagtataa gtatcaagat aaagtgctaa caagtcttta gtctcatgat
    13321 gtttcatctc attaatcaga acttgtgagc gaatagctgc tgggagtcct gcaagattct
    13381 ccttgtgtgt tgcgaagatt tggctagcga cttgactagc ttctgcatca tttgagcgaa
    13441 tcatcatcga aacagccagc tgacgaacca attcatcctc atctgattct ccgtctttag
    13501 cttcaaaacc aagacggtca tagttatgac gagccaattt agcaaccagt cctttgaagg
    13561 ctgtttcagc atccgttcct tcatcaataa agcgctcaag ggctgaaatc acttgagaaa
    13621 cagctgaaac caccagataa gactcttcct tagcaagttt atcaagaact ggaagcaagt
    13681 ctgcataaga aatgtgccct gcctcagcca acaaacgacg ttcttgaaca atttgcagtt
    13741 tgcttgtgtt atcaagtgtc tctagctcag caagaacagc ttctaacaag tctccttgat
    13801 agtcggtaat atagtgggca gtattttcag tgttgagacg aagagctcct tcattttcag
    13861 caagaagagc tgcgtagcca gggatttcga tactttcagt ttcgagtgta tcaggcaagc
    13921 ctttccagtt gctattgagg ggcaccaccc agagacggtt cttgtcttcg ttctcaccga
    13981 tgaagaattg tttttgtgaa atcttcaaga catcattttc aactttgaca gtaagaactg
    14041 ggtaaccagg ctgttccaac caagaatcca tgaaggctgc gacatcacgt cctgacgctt
    14101 gaccaagggc atcccaaagg tcactaccaa tggtgttgct gtattggtgt ttttcaaagt
    14161 aggcgtgcaa acctttagca aaatcagcat ctcctagcca acggcgaagc atgtgcatga
    14221 gacggcttcc tttggcatag acgatagcgc cgtcaaagag tgtattgatt tcatctggat
    14281 gtttaacttc gacgtggaca gactgaacgc catcagtagc gtcacgttca agagcaagag
    14341 gtactccacc tgtttggaaa tcttcaaaga tattccagct tggttcgatg gtatccacac
    14401 agacgtattc catcatatta gcgaaacttt cattgagcca aaggtcatcc caccatttca
    14461 tagtcacgag gttcccaaac cattggtgag ccaattcatg ggccacaaca agggcaactt
    14521 gttgacggct agcaaatgta gagttctcat cgacaaccaa gtaaacttca cggtaggtca
    14581 caagacccca gttttccata gcaccagctg agaagtcagg aagggcgatg tggagagatt
    14641 gaggaattgg gtacttaact ccatagtaat cttcgtaaaa ctcgatagag cgaacagcga
    14701 tatccagtga gaaatcaaga tttgaaagtg gatgtgcttt ggttgagtag acacctacca
    14761 gggtaccatt tttagtttta gcggtcaccc cttgcaaatc accagcaaca aaggccaaca
    14821 agtaagaaga catgcgaggt gttgtctcaa acttccagat acctgtttcc ttacggtttt
    14881 caacatcgat ttctggcatg tttgacaagg ccaattcacc ttctgcttgg tcaaagcgaa
    14941 gagagaggtc aaaagttgct ttggcttcag gctcatccac acatgggaaa gcttcgcgcg
    15001 caaaatggct ctcgaactga gtagacaaga cctccttctt gactccatca actgtataat
    15061 aagaagggta aatccctgtc atgttgtctg taattttacc agaaaaggca agaaccaatt
    15121 caacttgacc agcctcagcc aattcgatat gaagggcttc attgtcatgg tcaactgtaa
    15181 atggacgagc ttgacctgca acttctacag aggtgatttc caagtctttt tggtggaggg
    15241 agatgcggtc actctgtgct tgaccagtga tggtcacttt cccagaaaaa gtcttggtct
    15301 cacgactcaa atctaaaaat aaatcataat gttcaggaac aaattgctta ataaaatgtt
    15361 caactgcttg catagttttc tcctattcta agtttaagag ctgctgctct tcttcaaaca
    15421 aacttattat atcatatttt gcttgagaaa aaaggattgg acagcgattt ccaaaacttt
    15481 cttccttcta atagtctaca gtgggtagac cttgcgaaat tcttctaaaa caaccttgct
    15541 ttcaggtgta aaagtcagcc ctacttcacc caaacagtct gtgaaaaagt cctcatgaac
    15601 ctggcgccaa taggcaagtg atttgtctcc ctcaccttcc ttaaaggcat gttgcgcaga
    15661 aacttgattg aagagttcaa cagaaacctt tgtaatttcg acaacgcaga cagcctgatt
    15721 ttgactatct aaaatgacat cgaaggtccc tacttgggga agtggttcgt cttctagtac
    15781 atagaggtca taggctgatg ctgttgctgt cttttctcct ttaaacacca aatccgctaa
    15841 aaggtctggt tcaactccaa aagcccaagc atcgatttca tctccgatca aaggattgat
    15901 ttgcttgtat ttattccaca tttcttgcgg tatcatgggt actcctttgt aattttttac
    15961 tttcttcttt tatgtgttta agatgatctg gatggtcaat ctctaaatca aaaatctctg
    16021 gaatagaact gtagtggata atgcacttga cacccaactg attcattttt tgtatgaaag
    16081 aagtattcag atagcctgct acagcaaaat taatcttgtt ctttcttgct ttatcctgca
    16141 tatctcttag catatctaac attattggac tttccatatc atgccattga ctgtttctca
    16201 tagtcgcaaa aacaaaggaa gtcaaatcat tcattccaac tacaatcttt gaaatgcccg
    16261 tttccagtat actagataag tcaaaatacg ctgacggtaa ttcaatcatc gttccgactt
    16321 tcccagtaaa gccatactga cgcaatactg taatagcttg ttttaattgg tcggcatcat
    16381 tgacaaaagg aaaaataaga gacagattgg gattggtttg ataaacttct gtaacgacat
    16441 gtgattcagc ctgaaattca tccaaacacg ccagtaaacg cctggttcct ctatagccaa
    16501 acaagggatg cccttcgtca aaaaactctc tagtccccgc taaacaattg gcttctgtat
    16561 tcgttaattc agaaaaacga taccaaactt ccttatctaa gtaaaaggag caaatagtat
    16621 caagataatc ttttacaaat tgctgacaac ttgctaatat gatgttttga ttaagctctc
    16681 tcaataagta ttccccacga atcatgccga cgtggtgaaa tagttgagga taaatttttt
    16741 caagaatttt ttcgccacta agggcaagtt gatttctcat agccattcac ctcgtcttaa
    16801 aagatacaat atcatatata attatatcaa aatttcctct agttcaaaag cttcataagg
    16861 ttcagataac tcatatccta aaaattctgc tacttttttg gaagcctcat tatgagcatc
    16921 ccaaagtgga aacatatctc tatttaaact ctctagaatc attgcagcac caagcttttt
    16981 agcaaatcca tttccttgtt cgtttggtct agttgcaact tccacttcaa ctgctttacg
    17041 gtaaactaac cctgaggaaa tcccagcaat cagttcatta tttttaagaa tcacaaagcc
    17101 aaatccacct tttaaaacaa aatcctgata ggactcaaaa tccccctgta aatcctgtga
    17161 ccattcttcc gtagaaaagc agttataaat atgattatca ataggcacaa tattgtaacc
    17221 ttcctctaaa tgagtaacta gattatttaa aaattcaact tgaaaatttg ccttatcttt
    17281 aaaagaatag cgagtaaaag aatttagccc cacttttcga tgcagaaaat cttgccaatt
    17341 agtattttct gaaataataa tcttataatc taaaccatat tttctaacaa aatcttccca
    17401 aaatctggaa tccaactccc ctgctaggta tagaaaattc ccaacatcat aaaagcttgt
    17461 tgtcctatca ttgtttcggt agacagttcc aatttgagat ttaagaccat agagaaccat
    17521 atttttcttc catgttttaa atagttccat tatccctcta cttcaccttc caattcatgt
    17581 aagaagtctt gtccagttct ggaaatccta ataattcaga cttaaccttc aagactaatg
    17641 gcgatgcatt ttcttctgta atctcttgaa tatccatcca aatatatcca agtgaatcat
    17701 tcgcaccatc agacacagct tccgaagtcg taacttgagg tgcactctca ttcatttcaa
    17761 catcatacaa ggccatgaca tggtgaacca taaaatttgt taactcttct ctgacgaaaa
    17821 catcgtagat tcgaggatta gagtagcttc taacagtaaa tcccgtctct tccataactt
    17881 ctctagtcag cgtttccgtc agtccttcac caagttgctg actgcctcca ggtagatcat
    17941 accgatgttg ataagggcct ctcgcttttt caatgcaaag taacttccca ttttctaagc
    18001 aaacagcgta gactccaaag tgttttttga tttccatcca actcctccta cttcaaagac
    18061 cagccaccat ctattgtcaa gatttgtcct tgcatggcgc tcgcttttcc acttgctaaa
    18121 aaaagactaa gctctgctat ttcctctggc tcaatccagc gcttgattgg ggtttcacta
    18181 gccacccagt cagccaaacc acctggttca aaatccgcag cggtcatagc tgtcttgact
    18241 gctcctggag cgatcccaaa gacctgaatc ccagcttcag catagtctag agccaactgc
    18301 ttggtgaagc cagccaaggc atgcttggat gaagtatagg cgtgaccacc tccacctgct
    18361 aggctagaag caatggaaca catattgatg atgattccct ttttattttc cagcatttgt
    18421 gtcaaataat accgagtcaa ctctactgga ataatgtagt tgatttcaaa aatctcttga
    18481 atgtcctgcg ccgtttgttc caacagtggt ttgtaatcat ccaaaactcc ggcagtatta
    18541 cacaaaacat ccacctgagg gcaccagtca aaaataggtt ccaagtccaa ggtcaaatct
    18601 ctctgtaaaa agtgaaaatc accctctaag agtggctttt caccttggtc aactccataa
    18661 acttgatagc ccttctctaa aaagaggcga gcttgagcca atccgatccc tgaactcact
    18721 cctgtaatga gtacacgttt agtcatgcac ttctacccaa tccgttgcca aaacatcaca
    18781 agctgtcggg ctccacatgg aaaaaccttc tccttctcca gacacgttga ttaggaaata
    18841 gggtgtcact tcaagtgcaa gcccattttg ctcgatggta tcaaagagtt ggacatagtt
    18901 ttcagcacct ccccaaccag ttcgtacata ttttttctta gcctttaagc caggtagaat
    18961 ttcttcaaat gtcatgtttt tctcctttaa tgtcaataat tctccccttc attataacaa
    19021 aaaaaccgct ttaaaacggc tttttgactg tgatttattt acatcttctt ctacttacgg
    19081 caaattattc cctgcagcaa gataaatttc ataccattct tttcttgtta agctaaagtt
    19141 tgccgcttgg ctaacttctc tcaagtgctt aggatttgtg gtacctacaa ctgcctgcat
    19201 ttttgctgga taacgcaata tccaagaaat ggcaatagtt gaagaggtta ctccatattt
    19261 aatagctaaa cgatcaagta cttgatttaa agcttgaaat ttctcatttc caacaaaatt
    19321 ccctttaaaa tacccgaatt gtaagacaga ccatgcttga atgaccacat cgtgtaattt
    19381 gcaatattca aaaatgctgc catctcgcat agctgcttga ctatcttcca tattaacatg
    19441 aaaagctgat tcaaatcctg gagtaaaagc cgcactcaat tgtagctgat taacagctaa
    19501 cggctgcttg acatcttttt taagcaactc catcatcata ggattttgat tagaaactcc
    19561 aaaatctcga actttacctt gtttataaag gagatcaaag gcttctgcta cttggtcaga
    19621 ttccatcaaa gcatctggtc gatgaaggag caagctatct agatgatcaa tcttcaatct
    19681 ttgcaaaata ccgtctactg attttataat atagtcctta gaaaaatcaa aataggtaaa
    19741 ttcttcaatg cgaatgccac atttggactg aatccacatc ttttctctta aatctggacg
    19801 attttttagg acaagaccta acagttcttc acaacgacca cgaccataaa tatcagccaa
    19861 gtcgaaggca ttgattccaa cagaaagtgc tgtttctaca agctcttcaa cttcttttac
    19921 agacttatct tttattctca tcattccgag aacaatttct gataattctt tgtcatcttg
    19981 accaagagtt atgtatctca tcaaattttt ctcctttaat ttctaacatt cttcccttca
    20041 ttataacaaa aaaccgcttt gcaacggctt tttgactata cttcactcca ttttatcttc
    20101 ttaaacccac ggaacaagac aaagattcca ataaagagga cagctaaagg aataactttt
    20161 gtaaggaaaa catttgaaat tcccatccac tcataataac ggagcagaga acccaccaca
    20221 agatgggcaa taatcatact gacaaatgga cgaaagaccg cttctttcca attccaaata
    20281 ccgataacta gcgaaatcgt aaagacagac aaactatccc agggagccgg aatataaaag
    20341 gctccttctt gtatgaagct tgccattcct acatatccta aaacaactag aagaactata
    20401 gtcccaacaa caatgtaagt gccaattttc attttaggag aatcttggac taaacttctt
    20461 cgtaaaattg tggccacaag tccaaatcca atcagaaaaa taagaagttg ccctaaaaat
    20521 gtgagcaaat tgactgttaa gagaggacct ttagaaaaat cacttagtag ttgataataa
    20581 cgtaataccg ccaggacaag aattggcgtc aaaagggact ctttgataga actgcgaggt
    20641 gctcccttga gaatctcttt cattattttt ttaggattct tacctagata atcctctgca
    20701 ctcatgccat ctcgttctgc ttctgagaaa tctagcatca tcaaatagat ctgctctctg
    20761 agatagtctt catcatagag aaatccagca agattaaaac tttcccacaa ctcctcaaaa
    20821 tacttttgat tctcctcaga aaactcatgt agcaaagcgc ttgtttcttc gtaatacttc
    20881 attttcttca tggtttaacc cccattctta atcccttcta ctttttgact caaatcgtcc
    20941 cattgttgcc aaaagactga gacacgctct tctccttctt tcattaatga aaaatacttc
    21001 cgatctggac catctggcga cgggcgcatg tcgcctctta tccattgatt tttttctaac
    21061 ttttgcaaca aaggataaat agttcctgga acgatagtat caaatccagc ctctcgcaaa
    21121 gtctgaacca actcataacc ataccgctct ttttgaccaa tcatatccaa gacacaacct
    21181 tcaagaacac cttttaatag ctgagtttct ttcatcactt ctcccttcta atctattttg
    21241 taatacctac tagtaacttc acctatagta tattacttct acactagttt gtaaagcata
    21301 atagttaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcccta ccgtatgtat
    21361 ggtt
//
LOCUS       DAVKHZ010000031        21204 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000031 DAVKHZ010000000
VERSION     DAVKHZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.031"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(172..450)
                     /locus_tag="VWW99_001067"
     CDS             complement(172..450)
                     /locus_tag="VWW99_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538922.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAVYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(724..2295)
                     /gene="rlmD"
                     /locus_tag="VWW99_001068"
     CDS             complement(724..2295)
                     /gene="rlmD"
                     /locus_tag="VWW99_001068"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9538923.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIP"
     gene            complement(2369..3343)
                     /locus_tag="VWW99_001069"
     CDS             complement(2369..3343)
                     /locus_tag="VWW99_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9538924.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3417..4031)
                     /gene="pmp23"
                     /locus_tag="VWW99_001070"
     CDS             complement(3417..4031)
                     /gene="pmp23"
                     /locus_tag="VWW99_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9538925.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLTLAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4031..5005)
                     /locus_tag="VWW99_001071"
     CDS             complement(4031..5005)
                     /locus_tag="VWW99_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9538926.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5013..6269)
                     /locus_tag="VWW99_001072"
     CDS             complement(5013..6269)
                     /locus_tag="VWW99_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9538927.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENIVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6331..6762)
                     /locus_tag="VWW99_001073"
     CDS             complement(6331..6762)
                     /locus_tag="VWW99_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9538928.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            complement(6764..7366)
                     /locus_tag="VWW99_001074"
     CDS             complement(6764..7366)
                     /locus_tag="VWW99_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9538929.1"
                     /translation="MMDRIRQELEKGGAVVLPTETVYGLFSKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7350..8189)
                     /gene="prmC"
                     /locus_tag="VWW99_001075"
     CDS             complement(7350..8189)
                     /gene="prmC"
                     /locus_tag="VWW99_001075"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9538930.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADISQEALDLARENAKNQNLQ
                     IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDATDYLKDSGKIYLEIGYKQGQCVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8189..9268)
                     /gene="prfA"
                     /locus_tag="VWW99_001076"
     CDS             complement(8189..9268)
                     /gene="prfA"
                     /locus_tag="VWW99_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9538931.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9278..9757)
                     /locus_tag="VWW99_001077"
     CDS             complement(9278..9757)
                     /locus_tag="VWW99_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9538932.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(9747..10346)
                     /locus_tag="VWW99_001078"
     CDS             complement(9747..10346)
                     /locus_tag="VWW99_001078"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9538933.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10469..10651
                     /locus_tag="VWW99_001079"
     CDS             10469..10651
                     /locus_tag="VWW99_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9538934.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10788..12161
                     /gene="mnmE"
                     /locus_tag="VWW99_001080"
     CDS             10788..12161
                     /gene="mnmE"
                     /locus_tag="VWW99_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9538935.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12208..13143)
                     /gene="dapA"
                     /locus_tag="VWW99_001081"
     CDS             complement(12208..13143)
                     /gene="dapA"
                     /locus_tag="VWW99_001081"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9538936.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     IPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13199..14275)
                     /locus_tag="VWW99_001082"
     CDS             complement(13199..14275)
                     /locus_tag="VWW99_001082"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9538937.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINTVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14860..14932)
                     /locus_tag="VWW99_001083"
     tRNA            complement(14860..14932)
                     /locus_tag="VWW99_001083"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14897..14899),aa:Thr,seq:ggt)
     gene            complement(15032..15541)
                     /locus_tag="VWW99_001084"
     CDS             complement(15032..15541)
                     /locus_tag="VWW99_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9538938.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15539..15635)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15664..16095
                     /locus_tag="VWW99_001085"
     CDS             15664..16095
                     /locus_tag="VWW99_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9538939.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            16177..16554
                     /gene="mscL"
                     /locus_tag="VWW99_001086"
     CDS             16177..16554
                     /gene="mscL"
                     /locus_tag="VWW99_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9538940.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16596..17690)
                     /gene="hemH"
                     /locus_tag="VWW99_001087"
     CDS             complement(16596..17690)
                     /gene="hemH"
                     /locus_tag="VWW99_001087"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9538941.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVTLVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17845..19068
                     /gene="pepT"
                     /locus_tag="VWW99_001088"
     CDS             17845..19068
                     /gene="pepT"
                     /locus_tag="VWW99_001088"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9538942.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(19104..19733)
                     /locus_tag="VWW99_001089"
     CDS             complement(19104..19733)
                     /locus_tag="VWW99_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538943.1"
                     /translation="MNKANQLLEQIYEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            19698..19919
                     /locus_tag="VWW99_001090"
     CDS             19698..19919
                     /locus_tag="VWW99_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538944.1"
                     /translation="MDLFQELICLIQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(20190..21202)
                     /locus_tag="VWW99_001091"
                     /pseudo
     CDS             complement(20190..21202)
                     /locus_tag="VWW99_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6143 a         4430 c         3946 g         6685 t
ORIGIN      
        1 atttagtgat ttgttttaga gaatttgttg tgttattccg tccttatttt gccataaatt
       61 gttttaagtg ggacctctta tccttaccat ttttacctcc taattttgag tataaaaaag
      121 acgatagagt ttttaatttc tatcgtctga gatttacata ttaaattttc atcacaaaat
      181 aagactcttt aaagaatctt taaattcatt ggtgtgctgc ttacaatatc cattattaaa
      241 atggatacta taaacagcac accaactatt tattttttga gttttaaact ttggtagaag
      301 tcttgaactc cttatattta cataactatt tgcctctata actattatga cacgaaacca
      361 acaagcaagt tcaagcacct ccatatcttt attcaaatga ggactaagat attcacaaac
      421 tttatcataa acctctttat cttttttcat aaaaggggca tacaacttag caaatttatc
      481 ctaaaatttt ttgttgcctt tatcaatcat ttcaacactc ctttaaatat aaaaatgatc
      541 ctatcaatct ttatattgag cctattttct aattgatacc ttgcaagaaa ggaataatag
      601 ctgttcctat accaagtagg aatgtaataa atgcagaaag tcatatactg tcattctttt
      661 ctattaaatc attttgaagt gtctcaaagc atccatgatg gcttcttctt tttcaggtgt
      721 acactatgga ataatttgtt tatccttttt agacttgttg taatgttctc gtaattctat
      781 tccacatttc ttttttatct gtgcaatata taatgtcgaa acttttaatt caaatttatt
      841 ccaaacatat tctttgattt gagcatatgt tgctttgctc tccgcacttg tcaaattcat
      901 ctcatccagc tcaatttcaa cacttatgtg cttatcgaca tcgagtttgg acaaaagtgc
      961 taccgtctcg acgtgatgcg tttgaggaaa tagatccacc ggctggactt tcttcaattc
     1021 atatcccaat tcttggtaga gtttgatatc acgcgccatg gttgcgacat tgcaggagat
     1081 ataggcgatg cgatcggctc ctgtttgggc gcttgctttg ataaagcttt ctgtcaagcc
     1141 cttgcgtgga ggatcaacca agataacggt tggttgaatc ccatctttaa gccaattctt
     1201 catagcattt tcagctgtgt cacagacata gtgggcgttt gaaatattgt tcaactgagc
     1261 atttttttta ctattctcaa ccgcttctgg aatcagttca acaccgtaga cttctttgac
     1321 atgcttggcg actgataaac caatggttcc aataccagaa taagcatcaa taaccacgtc
     1381 atcttttttt aactctgcaa agtcaatggc tgtttgatag agtttctccg ccatttcagt
     1441 attgacttgg taaaaggctg ggccagcgat ttggaagtca tttcccaaca tctggtccgt
     1501 aatatagtct tgaccataaa gagtgcgcca ctccttacca aaaatcgcat tggtattctg
     1561 gtcgttgata ttttgcatga cagacacaat ctctgggaac tgcttgataa cttgttcaat
     1621 caattggtca acacgaaaaa cttttggacg agttgtcacc aaaacgacca tgatttgtcc
     1681 tgaatagtga ccacgacgca ccacaagatt ccgaatcaat ccagactgtt ccttttcgtc
     1741 ataaggtttt aaatcaaaac gacggagcag gtctcgaaga gctactacga cttggtcaat
     1801 gacaggatcc tggataaaga aatcttcaag gggcatgagg ttgtgcgaat tcttacggaa
     1861 aaatcctgtt tccaagacac cattcactcg acgaacgggc acctgcgcct tattgcgata
     1921 cttgactgga tgttccatac caagcgtttc agcaacttct acatctgcaa ttccagcaat
     1981 cttgtagaga ctgtccttga cttgcttggt tttaaacttg agctgttctg gataagaaag
     2041 gtgtcctaaa tccgcgattc ctgaacgcag gtaagccaaa tctagatctt gattacggtg
     2101 tggtgactgg acaaggtatt tttcaacttt tccaaagcca atctttttat tgaccttgag
     2161 gacacgcatg agaatttttt cactcggtaa agcattctct acaaaaaaga ccaaaccatc
     2221 taccttggca actcctgccc cttcatgggt caaatcaaca atttcaactt ctacaatatc
     2281 atttttcttt aacattcttt tctctttcta ttggccgaca aaaaagacgg caacgttacg
     2341 gttaccatcc attataccat gaaatttctt aagaaccaaa actcttgaag aagttgacaa
     2401 gggcttgcca gagggagctt aagaagttac caccgtcctc tagcgcctta tccgcatcaa
     2461 agttaaggtt gatattttta aaactgtcgc cagcttgtga tacgatgctt tgtttaaggt
     2521 catttagggt tttagtgaaa tctgcattgc tgaggatatc actctttgag agattcaagg
     2581 caaaattgat gatgatattg atctggtttc ctgttatgac ctgatcaagt ttgtaatttt
     2641 ttaaggtatc ttcaacaatc ttgcggatat cttcttctgt cagatttccc ttgctttctt
     2701 tagctttggc gagtcctgac ttgatatcag ctagggcaac gtttaattta ttagcatcat
     2761 agcctgattt gtccttgttt tcagcattga tatctgacaa agcctttagc tcttcttgag
     2821 ccaaatcttt attggcttgc ggcaccttgg ctccattagc ctctagcgaa tagtagatcc
     2881 cagccaaagc actctcacct gtaactggaa tgggtgctgc tacagtgatt ttggcgtgtt
     2941 ccacacccaa agttacggct gcatttcggt acatatcctg agttacctta gtgatgtttt
     3001 caggtgtttc aatcttgacc tcaagtggcg atttgtcacc tagcttttga atcttggctg
     3061 atgaatacaa ctgtaagcta gagtcattgg ccacattcat gattttagaa taaacatcag
     3121 gtgtcatggt cttgagttct ttggtatctg ttgaggcatt gtagcccagt tttttaagag
     3181 tttgattttt ttggtcttca gatagggaag aacctaggac atattcaggt tggacatagg
     3241 tttcatcgat aactttttga acatctgttg ctgcatggac gctattcata gctgttactg
     3301 cccacaagat cgcagcgcta gtcagaaaga gtttctttct catagggaat ttcctccttt
     3361 acttctttag agtaatatat ctatcttaaa gaaaacttat aacaaaaaca cctggtctag
     3421 ccagatgttg aaaagagagt gaaacatttg atgatgtaaa ggttaagtcg tacctgtcta
     3481 gaataataat agtttcctcc atttacatag agttcagcac cgtgaaaaat ggaaatgggg
     3541 tgaatataac tataagtctt tccagtccta ttaccaagca agggggcaac agtctcacga
     3601 gagtactgtt tggctagagt caaggtattt tccttgccat tttgggcgat aaaatcgata
     3661 taggcaggtc caaaattata ggcttgaaca gctgtccaga tatctacacc cttcttctgc
     3721 gccagataga gattgcctgt cagagtttga atgccttgcc gaatgctaga ggcattatca
     3781 ttgatggtgt tggtggaacc acttgcagac tcactagact gcataacatc gccttctttt
     3841 ccttttgttt cagtataaat catagcaagc acaagctctt cgtttgctgg ggtgtctttt
     3901 tcactcaata tttctcgcac catgggttga taggtcatga cttgtttgac atcttgatga
     3961 acgcggtaag ctttatagcc agcaaaaagg aagactgcta gtacaagcac tcttcgaatt
     4021 cgtttaaaca ttatttactt tggatatcct cgatattttt gattaagata gagtaggttc
     4081 cattttcgtt ttggataaac tcaacagact cggcgtcttg atagacgtta ttgggaacga
     4141 tgagctcaat tccatttgat aaggagagtt tttggttttc aaatttcttt aattggcgac
     4201 tggcatcaat ttcatcaaat tgaacaggtt ctggtacggc ttctttgact tggtcaataa
     4261 agctcaaacg agccgtcaga ttgttgtcaa aaaggtcatt agccaatttc tcaggtgaca
     4321 attcattgct ttcttctagg ttgttgaaaa tagctgattt gaccttggat tgaaattgaa
     4381 aatcatctgt gttaaaagat tcagcaattc tctgggctgt tttttccagt tccttgatag
     4441 attttttagg agaaatctta ggagcgacag caagaagatt atctgaaaaa tagttcaaaa
     4501 aagtcccgtt gtacttgatt cgtttttcaa tcaggtgata cttgcgactc tgaagattga
     4561 ccaccaaggc ctcgtcagca cccgttccaa atccaggcag gttattctga gtcagcttga
     4621 ttggattatc aacttctcct ccgaggtggg tcaaggtctc ccgcagggca attcgcaaga
     4681 aagcgaaatg ttctacacct tctttagaaa attgaacaaa aatcaagtca ttggtcttga
     4741 gattttcaga aatgctaaac tcctctttcc agagattagc cagcgttact gatgtctcca
     4801 acaaatcgtc tgtaatatga ttgaagaagg gattttcttc ttcgaaaatc ccagtcttgg
     4861 cttcatctga atacacatgt tcaatttttt tacgtaggta ttcttcgatt tttggagtaa
     4921 tattgagaaa cttatctgct aagaacagct cggtatcatc cggactgaac tggtgaataa
     4981 tggctttctt aatataaatg tccataaaag ttttattcct cgtataatgg gaaggcatct
     5041 gtcaattctt tgactgcact tctcacttct tctaatacag cctcattttc tgaattctta
     5101 agggttttaa tgatgagttc agccactttg cgactttctt cttcaccaaa tccacgtgca
     5161 gtaatagctg ctgctccgat acgaatccca cttgtcttga atggtgacaa ggtttcgtaa
     5221 gggattgaat ttttatttag ggtaatattg acttcatcca gcaagttttg agcaactttt
     5281 ccgttttcta caaccttagt cacatcaaca aggaagagat ggttttcagt tccacctgaa
     5341 ataatacgga aatcagggtc ttgcaagaag acatctgcca tagccttgct gttcttaatt
     5401 acattggcag catattcctt gaaggctgga tccaaaactt ctttgaagga aactgcctta
     5461 gccgccacaa catgctctaa aggaccgccc tgaatacctg ggaaaatagc tgaattgatt
     5521 tttttagcaa gttcttcgtc attggtcaaa atcaaaccac cacgaggtcc acgaagggtt
     5581 ttgtgggtcg ttgttgttgt gatatgagcg tatggaactg ggcttggatg aaggccagcc
     5641 gcaaccaagc cagcgatatg ggccatgtcc accatgagct tcgcaccgac agcatctgcg
     5701 atttcacgga attttgaaaa atcgataatt tgagaatagg ctgaagcacc agctacaatc
     5761 agttttggtt ttacttcttg ggcttgtttc aagatagcat caaagtctaa gagttccgtt
     5821 ttaggatcaa cactataaga aacaaagttg taggtttgac cagagaagct aacaggagcc
     5881 ccatgagtca aatgaccacc tgatgccaaa tccattccca taaccgtatc acctggctca
     5941 atcaaggaca tgtaagccgc acagttagct tggcttcctg aatgtggttg aacattggca
     6001 aatttagcac cgaaaatttc ttttgcgcgt tcaatagcaa gagtctctac aacgtctact
     6061 acatcagttc caccataata acggcgtcct gggtaaccct cggcatattt atttgtcaag
     6121 atagaccctt gagctgccat aacagccttg gaaactatgt tttccgaagc aattaactcg
     6181 atattatttt gttggcgttc ttcttctttg gcaatagcat tccagagatc agcatcatat
     6241 gctttaaaat catctttgtc aaaaatcata ggtcttctcc tttattgtgt gactagtcca
     6301 ttagtttgat tttacaataa gaaaatcaaa ctaacagatg cgaataaacc gtttctgcat
     6361 tttatcacaa gtatagccaa ctttttcata aaatgcatga gcacccagac gatgattggc
     6421 agaatttaag cggataaacc cataaccaca tctttttgct tcttcttcca acccttgtag
     6481 taaactttta ccaatacctt gaccttgcgc ttgaggtgaa actgctaaag ctaagatatt
     6541 aaatcctgct ttggaataga gtgattcgta aacttcagcg tggacatatc caagtaagac
     6601 atgattagct gcatcctcat agccaagtag gaaatgatgg gaatcctgag acagtctagc
     6661 tagttggcta gccgtttcct ctggactaaa agtataaccc aaagcctctt ggttgatgtc
     6721 acatatcgct ttcacatctg tttcttgcaa atctcttagc atttcaagcc tcctcaaaag
     6781 aaatctctgg caaccgagca agaatatctt ctcgtttaat tgcgccttgg cgtaagattt
     6841 tcaccttgtc tccagacaaa tccacaatag ttgaatcctg tccagttaga aaagcatcgt
     6901 cttccagacc cagaacctct tggtcaaaat ccttcagaat ttgttcaaag gttacaccac
     6961 ttgcctgacc tgagatattg gcagacggcc caatcaaggg acctgtctct cgaattaaat
     7021 ccagtgtgat agggtgactg ggcatccgaa atccaatagt tgcaaggtca gaatttaccc
     7081 aatagggaac tcggtcattg gcttcgagaa taatggtcaa gggacctggc aaaaaggtct
     7141 ctacaagttt ttgtagataa gctggctgat tctttgaaaa gtgcaagatg tcctcgaaag
     7201 aggcgatatt gagattgagt gccttatctc taggacgacg tttgagttgg taaacatggt
     7261 caactgcttt ttcatctaag gccttggaaa aaagaccata aacagtctct gtaggtagaa
     7321 cgacagctcc acccttttcc aactcttgtc taatcctgtc catcatcaac cacaaccatc
     7381 ctattttgac caaattggtc tttgagtgtt cgaactcttt tttcaggaag atgttttcta
     7441 aaaagttcgg gaacacattg accttgcttg tatccaattt caaggtaaat cttaccacta
     7501 tctttgagat agtctgttgc atcttccgca attctacggt aaatagctag gccatcctcg
     7561 tctgcaaaga gagctagatg aggctccgaa tacaagacat tcaagcctac ctctgactca
     7621 tcttcacgag agatataggg tggattggaa acaattatat catatttttc agaaatttct
     7681 gtaaaacagt cagatttttt taaaaatatt tgaagatttt gatttttagc attttctcta
     7741 gctaaatcta aggcctcttg agaaatatct gctgctgtca ctgaccaatc tggtctattt
     7801 tttgctagag cgagagcaat agctccacta cctgttccaa tatccagaac tgagagattc
     7861 gtctcaagat tttcagttag aataagctcc actaactcct ctgtttctgg acgaggaatc
     7921 aaaacccgtt catccacctt taaatgcatt ccataaaaat ctgcctgtcc aatgatgtac
     7981 tgagctggct tgtgagctgc tagttgctgg taaatatctt ctacaaattg tttttcttcc
     8041 tctgttgtca cctcctgctg gagggcaaaa ataaagtctg taaaagatag atttttcaga
     8101 ctacgataga caaaagagag gctttccgct tcctctcctt gtcttatcaa ctcttcttca
     8161 aaatttgaaa ataattgagc taatttcatt atttgtttaa ttcttctagt ttttgtgttt
     8221 ggtcataaag caccaaggca tccacaactt cgtccaattt accagacaaa atcgtatcta
     8281 gtttttggag ggtcaagccg atacggtggt ctgtgacacg gttttgtggg aagttataag
     8341 ttcggatccg ttctgaacgg tcaccagtac cgattgtcga cttacgctca gcgtcttgtt
     8401 catcctgagc aatctgagca aagtggtcag cgacgcgtgc acggataatc ttcatagcct
     8461 tctcgcggtt cttctgctgg gtacgttctt cctgcatctc aaccttgata ttggttggca
     8521 agtgaacgat acgaacggca gtcgcaacct tattgacgtt ctgtccacca gcaccagagg
     8581 cgtgatagat gtcgacacga aggtcttttg gatcaatgtc gtattcaacc tcttcaactt
     8641 ctggcataac aagaactgtc gctgtcgaag tatgaacacg gccttggctt tctgtcacag
     8701 gaacacgttg cacacggtgg gcacctgatt catacttaag cttagagtat acagactgac
     8761 ctgaaaccat agcaaccact tctttaaaac caccgacacc attcatagag gcttccatga
     8821 cttcaaagcg ccaaccttgg gcttccgcat acttttggta catagttagc aaatctccag
     8881 cgaaaagtgc cgcttcgtct ccaccagctg ctccacggat ttcaaggatg atattcttgt
     8941 catcgtttgg atcctttgga aggagcaaaa ttttcagttt ttcttcatat tcttcttttt
     9001 cagccttggc atctttgagt tcttgcttgg ccatttcttc caagtccgca tctccgcctg
     9061 attccttaat catctcttcg gcatcgacga tattttgaag gacttgttta tactcacggt
     9121 aggctattac ggtgtcacga ttggaagctt cttcttttga aagctccata aaacgcttgg
     9181 tgtctgaaac gacatcaggg tcactcagca attctcctag ttcttcataa cggtcttcta
     9241 cagcttgtag ttgatcatag atgttcattt tttctcctta tttctcaatt gttaaatcat
     9301 agattgctac tacttcattc tcggatattt ccccagtttc tttaaatcca taactgagat
     9361 aacaaaatct tgcctgttca ttttctggtt cataagacaa ccaaagttta ttgcttaaac
     9421 ctgctggcgc tgttcgaaca tagtctagta ctttatccat aattggttta aaatatcctt
     9481 gattttgaaa attcttatca atcataaaac gaaatagtaa ataatttcca ctactaattc
     9541 cgatcttttt atcataagct atcatcacaa aacctataat tacatcatta tcataaactg
     9601 ccaatggagc tacaaaatct ccatttttag tgtagacgta tgcttcagct aaactaattg
     9661 cgttggttgc aatgaattgt ttttgatatt ccttgacatc caaatttaaa acatcaaaat
     9721 aattttccat tgtaacatct cttagttcaa ttgtcatagt tttgctcctt gttagaggtt
     9781 atcattggcg caaaataatg tttacggcaa accgagatat aggtttcatt accaccaatc
     9841 tggatctgtt ctccatcgta aacgggcagt ccatcctgtg ttcgcaacac catggtcgcc
     9901 tttttcttgc aatactgaca gatggtcttg atttcgtcaa tcttgtccgc taaaagcaag
     9961 agatatttgg aaccttcgaa caattcattg cgaaagtcat ttttcaagcc aaaagccatg
    10021 acgggtatgt ctaactcgtc cacaacacga gctaggtcgt aaacatggtg gcgtttaaga
    10081 aactgggctt catcgaccaa aacacagtaa ggtttttctg gtaggtctcg gatatagcca
    10141 aagatatccg ttgtttcctc aatcgcaagg gcagggcgtt tcatgccaat tcgactcgac
    10201 acatagccaa caccgtcacg cgtatccaga gccgaggtca taatgacaac accttttcct
    10261 tgctcctcgt agttataggc cactttgaga atctcaatcg ttttaccaga gttcatggtc
    10321 ccataacgat agtacaactg tgccatgttt cttgcttcac gtccatttct aaatttttgc
    10381 tacattctag tatatcataa ttttcttaag ctttaaacgg caaaatgtgg taaaatggaa
    10441 gaaatcaaaa ctagtggagg aagctattat gccatttgta cgcatcgatt tatttgaagg
    10501 acgcacgctc gagcaaaaga aagctcttgc taaggaagta acggaagcag ttgtccgcaa
    10561 cactggagcc cctcaatctg ctgtccatgt catcatcaac gacatgccag aaggaactta
    10621 cttcccacaa ggggaaatgc gtactaaata agctagctta agcagaattg cttaggcttt
    10681 ttcaatctcc aagtagcatt cattgaagaa atatcctaaa tttgttacaa tttgaaaaga
    10741 aacttggaga atttccaaga aaagagctat taattaaagg aaacattatg attacacgtg
    10801 aatttgatac catcgctgct atctctactc cactaggtga aggggctatt ggtattgtcc
    10861 gcctgagcgg aacagacagt tttgctattg cgcaaaagat ttttaaagga aaagacttga
    10921 acaaggttgc cagccacact ctcaactacg gtcacattat tgatcctctg actggtaaag
    10981 tcatggacga ggttatggtt ggggctatga agtctccaaa gaccttcact cgtgaggata
    11041 ttatcgagat taacacccac ggtgggattg cggtgaccaa tgaaattctc cagctagcta
    11101 ttcgtgaagg ggctcggttg gcagaacctg gtgaatttac caaacgtgct tttttaaacg
    11161 gtcgcgtaga cttgacacag gcagaggctg tgatggatat catccgtgcc aagactgaca
    11221 aggccatgaa cattgcggtc aaacaattag acggctccct ttctgacctc attaacaata
    11281 cccgtcaaga aatcctcaat acacttgccc aagttgaggt caatatcgac tatcctgagt
    11341 atgacgatgt tgaggaagcc actactgctg ttgtccgaga gaagacaatg gagtttgagc
    11401 aattactaac caaactcctt aggacagcac gtcgcggcaa aatccttcgt gaaggaattt
    11461 caacagctat catcggacgt cccaacgttg ggaaatccag ccttctcaac aacctcttgc
    11521 gtgaggacaa ggctatcgta accgatatcg ctgggacaac acgagatgtc atcgaagagt
    11581 acgtcaacat caatggtgta cctctcaaat tgattgatac agccggtatt cgtgaaacag
    11641 atgacatagt ggaacaaatc ggtgttgagc gttcgaaaaa agccctcaag gaagccgact
    11701 tggttctact agtgctaaat gccagtgaac cactgactgc gcaagacaga caacttcttg
    11761 aaattagcca agataccaat cgcattattc tacttaataa aaccgacctg ccagaaacga
    11821 ttgaaacttc gaaactacct gaagacgtta tccgtatttc agtccttaaa aaccaaaaca
    11881 tcgacaagat tgaagagcga atcaacaacc tcttctttga aaatgctggc ttggtcgagc
    11941 aagatgctac ttacttgtca aacgcccgtc acatttccct gattgaaaaa gcagttgaaa
    12001 gcctacaagc cgttaatcaa ggtcttgagc tggggatgcc agttgatttg cttcaagttg
    12061 acttgactcg tacttgggaa atcctcggag aaatcactgg ggatgctgct ccagatgaac
    12121 tcatcaccca actctttagc caattctgtt taggaaaata agaaaaatcc atgatccttc
    12181 attcggtcat ggattttatt gtctttatta gtaatctggt cttaagaccc ctgttacagt
    12241 tgccttagtt gcttcgtagt cgccatctac gacaaccttg ataatgcgtt tggcatcttc
    12301 ttctggtgct ggaataagag gtagacgagt gggtccagct tcaaatccca tatagttaag
    12361 aactgcctta actggagcag gacttggata agagaagaga gcattaacct taggaatgaa
    12421 tttacgctga attgctgcgg ctttcttcat atcgctttct gcaatggcag taaacatctc
    12481 gtgcatttca tccccatttg tatgagaggc aacagaaata accccatccg ccccaaggtt
    12541 catggcatgg aaagcatctc catcctcacc tgtataaatc aagaactctt caggcttgtg
    12601 ctcaatcaag taagccatat tagccaagct agtacattct ttgacaccga taatatttgg
    12661 atggtcagcc aaacgaagca tggtttctgg agtcaattcg accactacac gccctggaat
    12721 gttatagata ataattggta ggtcagaagc atctgcaata gccttaaagt gctgatacat
    12781 cccttcttga gaaggtttgt tgtagtaagg aacaatagca agaccagctg cgaaaccacc
    12841 aaattccgct acttctttga caaactcaat agagtcacgc gtatcattag tacctacacc
    12901 cgcaatcaaa ggaacgcgtc cattgacaac cttttgtaca gccgcaaaca actccaactc
    12961 ctcatcgtgg gtcaaagttg gactctcagc agtcgttcct gcgagaagaa ttccatccgt
    13021 atgatgggcc aataaatgct caatcaaggc tggaatagca tcaaagttaa tagaaccatc
    13081 ctcatgaaag ggggtaataa aggctgtaat gattttacat tcttttaaat cttgataaga
    13141 catgaaaaca cctctctatt tcaaagaagg agttcatctg aactcctaaa cgatatgact
    13201 attttaattc aaatttcaat tcggctgttg gacgaaccaa tccacgttca tgaagagttt
    13261 cagcaatctg aactgagttc caagcagcac ctttgagaag gttatctgaa acaacccaca
    13321 tgtgaattcc tttttctgca tccaagtctt tacggatacg accaacaaag gtatcacgcg
    13381 aaccaactgt attgatagct tgaggataga tttgatgagc tacatcatct tcaagaacag
    13441 cacctgggaa ggctgcgata gctgctttta cttcttcgat tggagccact tcttttgttt
    13501 cgatataaac agactcagag tgagctgaca agactggaat acgcacacat gttgcagata
    13561 ctgcaatgct atcatcttcc ataattttct tagtttcctt ggtcatcttc atctcttcgt
    13621 acgtgtaatc attatcagtg aaaacatcaa tttgtggaag agcgttaaag gcgataggat
    13681 aatgtttctt gtcaccacct gaaggcaaaa tttccgcatg caaatcacat ggtttcacac
    13741 catcattcaa gacttcacga agttcacgtt gtgtctcaag aattgctccc ataccagcac
    13801 ctgaaacggc ttgataagtt gaaacaatga tacggtccaa gccccatttt tggcgaaccg
    13861 gctcaagagc caccatcatt tggattgttg aacaattagg gcaggcaatg attccgttgt
    13921 gggcatcaag tgcatgagca ttgacctctg gaacaaccaa aggaacatct ggattttgac
    13981 ggaaataaga tgtattatcc actactacca cgccagcttt tactgcgtat ggtgcatact
    14041 tagctgatgt agaactacct gctgaaaaga gagcaatatc aactccttca aaagctgttt
    14101 cagtcgtttc ttcaattgta atatcttgat ctttaaattt caatgactta cctgctgaac
    14161 gtgcagaagc aaggtaacgg attttatcga ttggaagtgt tgattcttcc aacattttta
    14221 tcatctgagc accgacagca cctgtcgcgc cgactacagc aactgtatat cccataaata
    14281 acctctttcg gaattttcta aaaatttcta taatagaagt attatactac tttttccatg
    14341 aattgcaaag ctttttcatc attttttgga aaataaaaat cccagtcgct agactaggga
    14401 ctaagaggca tcttcatgtg atgagcaggt tcacacaact catcaaggtc cgctcctgcg
    14461 ttatgacctc cttatgctca atagtagccc gaagactacc tatcgactca tcgcatctac
    14521 tattctacta aagagaatga cttttgtcaa cagacgaaac tctttatact cttcgaaaat
    14581 ctcttcaaac ttcgtcagcg tcgccttgcc gtatgtatgg ttactgactt cgtcagtttc
    14641 atctacaacc tcaaaaacat gttttgagct gacttcgtca gttctatcta caacctcaaa
    14701 gcagtgcttt gagcaacctg cggctagttt cctagtttac tctttgattt tcattgagta
    14761 ttagttttat ttacacaggt gcataagaaa tatattggaa aaattggggg cagacataac
    14821 aaaaaacctt gtaaatcaag ggcttttctg tctatttaat gctaattgcc gggattgaac
    14881 cggcgacctc atccttacca tggatgcgct ctgccaactg agctaaatca gcttacttaa
    14941 gaagtatact atattcagaa aaacttgtca agtttcctta gaaattttcc ataggaaaaa
    15001 gccaggtaag aactacctag cttatcttct tctatttact taaatcaatt cgacgaccaa
    15061 ttttaccaat aataatcgcc ccaataatca atgaagcaaa ttcaccgatt cctgttgtaa
    15121 accaagtaag gaagaatggt aattctgcta caatatttaa ctctgcagcg atagtaaaca
    15181 tggaaattga aaagaggatt gaaaagaaga aatgatcttt tcgaatcaat ccgttaaaga
    15241 ggtaatcctt gctatatttt gcaaaaagcc aaacacctaa actgaggaat actaaggttg
    15301 aaccaccacc aacaaagaca tctagcagtc cgaagctaaa gaaattagca atcatacaac
    15361 cgatggtaac tccgatgatg tacttaggat tgtaaaaagc cataaagttc atcatttctg
    15421 aaatacgaaa ctgataagca ccatagctga tggcatttag gggcggtgtg acagtcaaaa
    15481 cgacgtagat agcagcgacg attgcaatat ctgcaacatc acgaatagtt aattttttca
    15541 tcttttctcc tttggcggtt gcccgcgtgt aatatgcttg gtgaaagaag ctaagcacca
    15601 agggttgatt cattcaacct tactagtata gcacaatagc ctgctttatg ctatacttaa
    15661 agcatgaaaa aacctattca aaaatttttt aacaatgaaa tcttagccta tctctttttt
    15721 ggccttgcga caactctagt ttcgattctc tcacgactag tcatttatca actaagtcat
    15781 caggaactcc ttgctacggc cttggcaaat attatcggta tcttatttgc ctttatcaca
    15841 aatgatacga ttgtatttaa gcaaaaaaag aaaaatagac ttatccgttt agtgaaattt
    15901 tctcttgctc gcctttctac ttttctgtta gacttgtttt taacttttct ctttgtaacc
    15961 caatttcctc acatcatagg gcaattcgta actgaaaaca ttgataagat aaatgctatt
    16021 gaaacaataa ttgcacaaat attcattatt atcatcaatt atatcttgag caaggtctat
    16081 atttttagaa aataatgctc catttatgag ccttttaatt gccttctata atgatttagg
    16141 atacaatgaa acacgaaaca ttgaaaggaa aacagtatgt taaaaaatct aaaatcgttc
    16201 ttgcttcgag gaaatgttat tgaccttgct gtcggtgttg tcattgcctc tgcttttggt
    16261 gctatcgtta cttcacttgt aaacgacatt atcactcctc ttattttaaa tcccgctttg
    16321 aaagctgcta aagtcgaacg tatcgctcaa ctttcttggc atggagtcgg ctatggtaac
    16381 tttttaagtg ctattatcaa ttttatcttt gtgggtaccg ccctcttctt tattatcaag
    16441 ggcattgaaa aagcacagaa actgactggc ataaagaaag aaaaaactgc cgaaaaaaaa
    16501 ccaaccgaat tggaagtcct tcaagaaata aaagctctcc ttgagaaaaa ataatcgatt
    16561 aaaaggatct agcgcaaagc taaatccttt tttctttact ctttgggtaa cttgcctgct
    16621 ttttcaagca tttttttgat aagataaggc atcttaacat tctcccgccc ttttttctca
    16681 atcagttttt tcacaaattc tggcatttgt aaatcttcag attcagctaa aatgtttttc
    16741 gtctcgtctg aaggaactaa ctcatcaaag gtctcttctt ctgggagaaa ttccttaaac
    16801 tcttgatttt cattgactct aactgtattg accaaagcat caatcaaacg agaatctgta
    16861 tttggcattg gtggacgatg gtagtttacc ccaaattcct ggcataagtc ataacattct
    16921 acatcattgt caaacaagac ctcaatatgc tcactgatga aactaatagg tacaaaaata
    16981 taatggtctg gatgttctgt ctgttctctg agatactcca aaacatctgg tttaatccat
    17041 ggaataccaa tatcactctc actctgccaa gtgttggtat attgttctga actcaagccc
    17101 agtttttcag cgactagctt gctattttcg aaaatttggt ctatataagg atcaccaaag
    17161 tccaaggcaa aaatgggcac actatgggct gaaaagatga ctttaaagct gtcttgcttt
    17221 acttcttctt ttaaaatctt agcaatttca tctgcccaat agtttaagag cgcttcttcc
    17281 tgataccagt ccttaatgac caaaaattga atttgtttgc tttctaaaaa tttttcatag
    17341 cccatgacag agtagaaaga ataatggggc tccaaaatca agcaaataca ctgttcaatt
    17401 ccgtctgctt ccatttgacc gatcacatct ggaataaagg gactggaaaa cttattagca
    17461 aagtagacac tgtattcatt ccccagccta gcttctacca aggtaacctc ttgacgggta
    17521 attttttgca gaggcgtgcc tccaattcgt acataattat catagagagt ctgaatctcg
    17581 tggtcttggg gtctcacccc acgacgaata tttgtgaaaa aatcagctac accttcaaag
    17641 gtaatctctt ctggtgaacc gaaggtcatc attaaaattg cttttttcat gactgcttcc
    17701 ttgtgatgtt tttatcaagt ttattttatc attaattcat cgataagtaa acgctaacat
    17761 agggacattc tcaatcttct gtcttttgtt tttctcttct ttctatgata caatggaaaa
    17821 aataaattca aaaggagttt ttttatgact tatccaaatc tcttggaccg cttcttaacc
    17881 tacgttaagg tcaacacgcg ctctgatgaa cactctacta ctactccaag tacacagagt
    17941 caggttgact tcgcaacaaa tgtcctaatt cctgaaatga aacgtgttgg actgcaaaat
    18001 gtttactatc taccgaatgg ttttgctatt ggaaccttgc cagccaacga tccgtcttta
    18061 acacgtaaga ttggttttat atcgcacatg gatactgctg attttaatgc tgaaggagtc
    18121 aatccacagg taattgaaaa ctacgatggt ggtgtgattg aactagggaa ttccggtttc
    18181 aaactcgatc cagctgactt caagagtctt gaaaaatatc caggacaaac gctcatcaca
    18241 acagatggaa caaccttgct aggtgctgat gacaagtcag gaattgctga aattatgaca
    18301 gccattgaat atctaactgc tcatcctgaa attaagcact gtgagattcg tgttggtttt
    18361 ggtccagatg aagaaatcgg tgttggtgcc aataaatttg atgcagaaga ttttgatgtg
    18421 gattttgcct acactgttga tggtggtcca ctaggtgaac ttcagtacga gactttctca
    18481 gccgctggtg ctgaattgca tttccaaggc cgtaatgtcc accctggtac tgccaaaggg
    18541 cagatggtca atgcccttca gctagcaatt gattttcata atcaacttcc agaaaatgac
    18601 cgacctgagt taactgaagg ttaccaaggt ttttaccatc taatggatgt gacaggtagt
    18661 gttgaggagg cgcgtgcaag ctacatcatt cgtgattttg aaaaagatgc ctttgaagcg
    18721 cgtaaagcat ccatgcaatc tatcgctgat aagatgaatg aagaacttgg gagcaaccgt
    18781 gtcactctca acttgacaga ccagtactac aatatgaaag aagtcattga aaaagatatg
    18841 actccaatta ccattgctaa agccgttatg gaagatctag gtatcacgcc tattatcgaa
    18901 ccaatccggg gtggaacaga cggctctaag atttccttta tgggaatccc aactccgaat
    18961 atctttgcag gtggcgaaaa tatgcacgga cgttttgaat acgttagcct tcagactatg
    19021 gaacgtgcag ttgataccat cattggcatc gtagcttata aaggctaaaa agacgaggta
    19081 gctcagctac ttcgcctttc tttttattct actggttttt cttgatttcc agtagttgta
    19141 gaagattctg ttgttttatt ttctgaagtt gattcagcag gtttagaatc tcttgtattg
    19201 cttggtttgt tttcatcgct agcagtttca atgttagatt ctgcagttgc gtttggttgg
    19261 ttctcagcac tggtgttatc accatttgct tcagcatttc ttgctggact tgtttcttca
    19321 cttgcgctag cttttgactg gatttgatga ttcaaaacta gaatagcttt tgtcgattca
    19381 agtaaagctg ttttgtcttt actcttagca gaaagttgat ctaataatgc atccacctta
    19441 tcaaagtccg catcagatcc attattactt tctaaataag agtgaagcga catgagaata
    19501 tcgtagagtt tttgatagag tacaagtgtc tgaggatctt gctcagcatt ttccttttct
    19561 tgttgaaggg cgctagcgat acgagtcaaa acatctttta cctgactgtt tacttcatcc
    19621 aagtctgcat cagccttgtt tgtggcagct tttagatttt ctacttcttc tgccaaggat
    19681 tgtctgattc cttcttcata gatttgttcc aagagttgat ttgccttatt caaaagactt
    19741 tctacttctt ccttgctatc tgtcgcagat tattggttgc tatctaccat gtactcctaa
    19801 aacaggagag ttataatcca agattacaag gccttacaga aataagaaat ccagataaga
    19861 caatgttcgt ccaagacgct attcgcttcg cacagcagca cggattcaat atgctttaat
    19921 tttaaagttt aggtgtcaag acctcttttt agtgtgccca aaatttagag aagtaatcaa
    19981 tcaactaact tttatttttt tcaaactttc agtaaactga cctaaagcta actcaatctg
    20041 tctttgtaga tgcttctgct atcagctaga agttgatcta cttttgccaa gactgccttc
    20101 tcatcaaaag ttccaggttg atagttggat tgcagggatg gaatcttgtt tttcaaagcc
    20161 gcttcatatc ccttagtttg aaccttgatg tagtgattgt ggtcgccatg aggaatcaca
    20221 aaaccttctg aatcttcact tataattcga ttggcatcaa aaaccatgac catcttcttc
    20281 ctcatggtgg acatgtagtg acggattact taatacagaa ctagaagaac ttcctacctc
    20341 ttccgtgtta gagtgtgatg ggggattgtt aagagatgac ttaggaatat agtgatagtg
    20401 ttccccatgt cttactatat aagcatcacc tatatctctg acaatatcat taggattaaa
    20461 aacatatgcg actgctaatt cacctgccga caagtcactc tcaggaatga aatgatagtg
    20521 accaccatgt ggtactatcg tagattgaaa tagaatatga gcaaattgat aaggggattt
    20581 taaagtaatt tctaacaatg atttagaaac tatgatgtgc tattctaaat tcaactcact
    20641 atatataacc atcatcggta gtataacgtc cctgtaattt tactacagat acttctgcac
    20701 tagctccttt atcgtcttta ccatgttctt gtttttggcg attgatttca tcttttgttc
    20761 gtacattttc tgcatgagct tgatctttaa ggtaaacata atactttcca tctaccttaa
    20821 taatatatcc tcccttaacc taactgacaa tatcttgatc tttcggctga tagttggggg
    20881 ctttcattaa tggctcttca ctaaagagcg catcaaaagg aactttacca ttatagcagt
    20941 gataatgatc gccatgagaa gttacataac cttgacctgt aatcttaata acaatttgtt
    21001 ttgcttgaat tccttctttt tgactaacct aatctggagt caaattttca gtcttcttag
    21061 tgtctttatt actgtttaca tatgaaacac gatttttatc tgtattggcc tgttagctat
    21121 gttggttcag agcataaaca cacagactta aggaaaggat aacaacagat ccagctgcta
    21181 tatatttctt tttaaatttc ataa
//
LOCUS       DAVKHZ010000032        21093 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000032 DAVKHZ010000000
VERSION     DAVKHZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.032"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            102..2324
                     /gene="recJ"
                     /locus_tag="VWW99_001092"
     CDS             102..2324
                     /gene="recJ"
                     /locus_tag="VWW99_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9538945.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2499..4160
                     /locus_tag="VWW99_001093"
     CDS             2499..4160
                     /locus_tag="VWW99_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9538946.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4229..5008
                     /locus_tag="VWW99_001094"
     CDS             4229..5008
                     /locus_tag="VWW99_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9538947.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5120..6340
                     /gene="murM"
                     /locus_tag="VWW99_001095"
     CDS             5120..6340
                     /gene="murM"
                     /locus_tag="VWW99_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9538948.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6345..7577
                     /gene="murN"
                     /locus_tag="VWW99_001096"
     CDS             6345..7577
                     /gene="murN"
                     /locus_tag="VWW99_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9538949.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7656..8357
                     /locus_tag="VWW99_001097"
     CDS             7656..8357
                     /locus_tag="VWW99_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9538950.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8397..10244
                     /gene="uvrC"
                     /locus_tag="VWW99_001098"
     CDS             8397..10244
                     /gene="uvrC"
                     /locus_tag="VWW99_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9538951.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10234..11067
                     /locus_tag="VWW99_001099"
                     /pseudo
     CDS             10234..11067
                     /locus_tag="VWW99_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11251..12051)
                     /locus_tag="VWW99_001100"
     CDS             complement(11251..12051)
                     /locus_tag="VWW99_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9538952.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12222..12827
                     /locus_tag="VWW99_001101"
     CDS             12222..12827
                     /locus_tag="VWW99_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9538953.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12840..14240
                     /gene="pepV"
                     /locus_tag="VWW99_001102"
     CDS             12840..14240
                     /gene="pepV"
                     /locus_tag="VWW99_001102"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9538954.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14294..14872
                     /locus_tag="VWW99_001103"
     CDS             14294..14872
                     /locus_tag="VWW99_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9538955.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15032..15184
                     /locus_tag="VWW99_001104"
     CDS             15032..15184
                     /locus_tag="VWW99_001104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9538956.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15227..15319
                     /locus_tag="VWW99_001105"
     CDS             15227..15319
                     /locus_tag="VWW99_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9538957.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15490..16815
                     /gene="brnQ"
                     /locus_tag="VWW99_001106"
     CDS             15490..16815
                     /gene="brnQ"
                     /locus_tag="VWW99_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9538958.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16913..17950
                     /locus_tag="VWW99_001107"
     CDS             16913..17950
                     /locus_tag="VWW99_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9538959.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            17952..18413
                     /locus_tag="VWW99_001108"
     CDS             17952..18413
                     /locus_tag="VWW99_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9538960.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18431..19147
                     /locus_tag="VWW99_001109"
     CDS             18431..19147
                     /locus_tag="VWW99_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9538961.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19270..19695
                     /gene="rplK"
                     /locus_tag="VWW99_001110"
     CDS             19270..19695
                     /gene="rplK"
                     /locus_tag="VWW99_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9538962.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19903..20592
                     /gene="rplA"
                     /locus_tag="VWW99_001111"
     CDS             19903..20592
                     /gene="rplA"
                     /locus_tag="VWW99_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9538963.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         6337 a         3889 c         4894 g         5973 t
ORIGIN      
        1 aatatactat aaaatgttat aaaaaagcaa tctggataga gaaaacgtct aaatcatgtt
       61 ataatgaagc aatagaattc ttagaaagag tggatgtctt tttgataaca cctacttatg
      121 aatggcagtt tgccctgcag gtagaagatg cggattttac aaagatagcc aagaaggctg
      181 gactgggtcc tgaggtggct cggttattgt ttgagagagg gattcagaac caagaaagtc
      241 tgaagaagtt tttagaacct tccttggagg acttacatga tgcttatctg ctccatgata
      301 tggacaaggc agtggagcgg attcgtcagg ctattgaaga aggggaaaat attcttgttt
      361 atggagacta tgatgcggat ggcatgactt cggcttctat tgtgaaggaa agtttggaac
      421 aacttggtgc tgagtgccga gtttacctgc caaatcgttt taccgatggc tatggcccta
      481 atgctagtgt ttataaatac tttatcgagc aagaagggat ttccttgatt gtgacggtgg
      541 acaatggggt tgctggtcat gaggctattg cattggctca gtctatggga gtagatgtca
      601 ttgtgacaga ccatcattcc atgcctgaaa ccctgccaga tgcttatgct attgtccatc
      661 ctgaacatcc agatgcggat tatcctttta aatatttggc tggttgtgga gttgctttca
      721 agttggcttg tgccctgtta gaagaagtgc aagtggaatt gcttgatttg gtcgctattg
      781 gaactattgc agatatggtg agtctgacgg atgaaaatcg tatcttagtt caatatggtc
      841 tggaaatgtt gggtcatacc cagcgcattg gtctgcaaga aatgctggac atggctggga
      901 ttgctgccaa cgaagtaaca gaagaaacgg ttggtttcca gattgctcct cgtttgaatg
      961 ccttgggtcg cttggatgat cccaatcctg ccattgattt gttgactgga tttgatgatg
     1021 aggaagcgca tgagattgcc cttatgattc accagaaaaa cgaagagcgc aaggaaatcg
     1081 ttcagtctat ctatgaagaa gccaagacca tggtggatcc tgagaagaag gttcaggtct
     1141 tggccaagga aggctggaat cctggggttc taggaatcgt ggctggtcgt ttattggaag
     1201 aattgggaca gacagtcatt gttcttaata tagaagacgg tcgtgccaag ggcagtgctc
     1261 gtagtgtgga agcggtcgat atttttgaag ctctggatcc ccatcgagac ctcttcatcg
     1321 cctttggagg tcatgcaggt gcagcgggta tgacgctgga agttgagcaa ctctcagatt
     1381 tatctcaggt tttggaagat tatgttcgtg aaaaaggtgc agatgctggt ggcaagaata
     1441 agttaaacct agatgaagag ttggatttgg aggcacttag cttggaaacg gtcaaaagtt
     1501 ttgaacgttt agctcctttt ggaatggata atcagaaacc tattttttat atcaagaatt
     1561 ttcaggtcga aagtgctcgt actatggggg caggtaatgc ccatctaaag ctgaaaattt
     1621 ccaagggtga ggcgagtttt gaagtggtag cctttggtca aggcagatgg gcgacagagt
     1681 tttctcaaac caagaatcta gagttagcgg ttaaattgtc tgtcaaccaa tggaatggcc
     1741 aaactgccct ccagttgatg atggtggatg cgcgagtgga aggtgttcaa ctttttaaca
     1801 ttcgtggaaa aaatgcagtc ttgccagaag gtgttccagt cttggatttt cctggagaac
     1861 tgccaaatct tgcggctagt gaagctgttg tcgtaaaaaa cattccagag gatattactc
     1921 agctgaagac catttttcag gaacagcatt tctctgctgt ctatttcaaa aatgatattg
     1981 acaaggctta ttatctgaca ggttatggga ctagagatca gtttgccaaa ttgtacaaga
     2041 ctatttacca gttcccagag tttgatattc gctacaagct gaaagatttg gctgcatatc
     2101 ttaatattca acaaatcttg ctggtcaaga tgattcaagt atttgaagaa ctaggctttg
     2161 tgacgataaa agatggtgtg atgacagtca ataaagaggc gccaaagcgg gagataggag
     2221 aaagtcaaat ttaccaaaat ctcaaacaaa ccgttaaaga ccaagaaatg atggcgctgg
     2281 gtacggtgca agaaatttat gattttttga tggaaaaaga gtagaagtta ggaaagagtt
     2341 gggaaatcaa ctcttttttg aaaacagacc ttcattttga aaatcatcaa aaaaatggta
     2401 taatggtagg aaaagattcg gctgaaagta tcagaacttt tagaataaga gggtagaatt
     2461 gccctataat caagataaac taagattttg gaggaaaaat gagtaatatc agtttaacaa
     2521 cacttggtgg tgtgcgtgag aatggaaaaa atatgtacat tgctgaaatt ggagagtcca
     2581 tttttgtttt gaatgtaggg ttaaaatatc ctgaaaatga acaattaggg gtcgatgtgg
     2641 tgattccaaa catggattac ctttttgaaa atagcgaccg tattgctggg gttttcttga
     2701 cccacgggca tgcggatgcc attggtgctc taccgtatct cttggcagag gctaaagttc
     2761 ctgtatttgg gtctgagttg accattgagt tggcaaagct ctttgtcaaa ggaaatgatg
     2821 ccgttaagaa atttaatgat ttccatgtca ttgatgagaa tacggagatt gattttggtg
     2881 ggacagtggt ttccttcttc cctacgactt actccgttcc agagagtctg ggaattgtct
     2941 tgaagacatc ggaaggaagc atcgtttata caggtgactt caaatttgac caaacggcta
     3001 gtgaatctta tgcaactgat tttgctcgtt tggcagagat tggtcgtgac ggcgtcctgg
     3061 ctctcctcag tgattcggcc aatgcagaca gcaatattca ggtggctagt gaaagtgaag
     3121 ttagggatga aattacccaa actattgctg actgggaagg tcgtatcatc gttgcagctg
     3181 tttccagtaa tctttctcgt attcagcaga tttttgacgc tgcggataaa acaggtcgac
     3241 gtatcgtctt gacaggattt gatattgaaa atatcgtccg cacagcgatt cgtcttaaga
     3301 agttgtcttt agccaacgaa attcttttga ttaagcctaa agatatgtct cgctttgaag
     3361 accatgagtt gattattctt gagacaggtc gtatgggtga gcctatcaat ggacttcgta
     3421 agatgtcgat tggtcgccat cgttatgtag aaatcaagga tggggaccta gtctatattg
     3481 ctacggctcc gtctattgct aaagaagcct ttgttgcgcg tgtggaaaat atgatttatc
     3541 aggcaggtgg ggttgtcaaa ttgattaccc aaagtttaca tgtatcaggg cacggaaatg
     3601 tgcgtgattt gcagctgatg atcaatcttt tgcaacctaa gtacctcttc cctgtccaag
     3661 gggagtatcg tgagttggat gctcacgcta aggctgccat ggcagttggg atgttgccag
     3721 aacgcatctt cattcctaaa aaggggacga ccatggctta cgagaatgga gactttgttc
     3781 cagctggatc ggtttcagca ggagatatct tgattgatgg gaatgccatt ggtgatgttg
     3841 gaaatgttgt tcttcgtgac cgtaaggtct tgtcagagga tggaattttc atcgtggcta
     3901 ttacagtcaa ccgtcgtgag aagaaaattg tggctagggc tcgtgttcac acgcgtggat
     3961 ttgtttatct caagaagagt cgcgatattc tccgtgaaag ttcagaattg attaaccaaa
     4021 cggtagaaga gtatcttcaa ggagatgact ttgactgggc agatcttaaa gggaaggttc
     4081 gagataattt gaccaagtat ctctttgacc aaaccaagcg tcgtccagct attttaccag
     4141 tagtcatgga agcaaaataa tcgttgaaat aaacagagag aaagtcgagt ttcggctttt
     4201 tcttatagaa aaatagaagg agaaaatcat ggcagtaatg aaaatcgagt attactcaca
     4261 agtattggat atggagtggg gggtgaatgt cctctaccct gatgccaatc gagtggaaga
     4321 accagagtgt gaagatattc ccgtcttgta ccttttgcac gggatgtctg gcaatcataa
     4381 tagttggctt aagcggacca atgtagaacg cttgcttcga ggaactaatc tcatcgttgt
     4441 tatgcccaat accagcaatg gttggtacac cgatacccag tatggttttg actactacac
     4501 ggctctagca gaggaattgc cacaggttct gaaacgcttc ttccctaata tgactagcaa
     4561 gcgtgaaaag acctttatcg ctggtctttc tatgggaggc tacggctgct tcaaactggc
     4621 tcttacgaca aatcgttttt ctcatgcagc tagtttttca ggtgccctca gctttcaaaa
     4681 cttttctcct gaaagtcaaa atctgggaag tccagcctac tggagaggtg tttttggaga
     4741 gattagagac tggacaacta gtccctattc tcttgaaagt ctggctaaaa aatcggataa
     4801 aaagaccaaa ctttgggcgt ggtgtggcga acaggatttc ttgtacgaag ccaataatct
     4861 cgcagtgaaa aatctcaaaa aactaggttt tgatgtgacc tatagccata gtgctggaac
     4921 tcacgagtgg tactactggg aaaaacaatt ggaagttttt ttaacaaccc taccaattga
     4981 tttcaaatta gaagagagac tgacttagtt tgaacttcag cataggggga gtagaactaa
     5041 aataaaatat gttttcacta gacttttcaa acgaaagtag tagaatagta ataaaatact
     5101 ggaggaaaga gagtaggaaa tgtaccgtta tcaaattggc attcccacat tagaatatga
     5161 tcagtttgtc aaagaacatg aattagccaa tgtattacaa agtagtgctt gggaggaagt
     5221 taagtctaat tggcaacatg agaagtttgg tgtttacagg gaagaaaaat tactggcgac
     5281 agctagtatt ttgattagaa ctcttccgct aggctataaa atgttttaca tcccaagagg
     5341 acctatattg gattatgggg ataaagaact cttgaatttt gccattcagt ctattaagtc
     5401 ctatgctcgc agtaagagag cggtttttgt gacttttgac ccaagtattt gcctatctca
     5461 aagtttaatc aatcaggaaa agacagaatt tcctgaaaat ctggctatta ttgatagttt
     5521 gcaacaaatg ggagtaaggt ggtcaggaaa aacggaggaa atgggagaca ccattcaacc
     5581 tcgtattcag gcgaaaatat acaaggaaaa ttttgaagaa gataaacttt ccaagtcaac
     5641 aaaacaggct attcgaacag cacgaaacaa agggcttgag attcaatatg gtggactgga
     5701 actattagat tcattttcgg agttgatgaa aaaaactgag aagcgaaaag agattcattt
     5761 gaggaatgaa gcctattata aaaaattgtt agataatttt aaggacaagg cctatatcac
     5821 cttggccacc ttggatgttt ctaaacgttc gcaagaatta gaagaacagt tagcgaaaaa
     5881 tagagccttg gaagagacgt ttactgagtc gactcgaact tcaaaagtag aagcgcagaa
     5941 gaaggaaaaa gaacgtttgt tagaggaatt gaccttcttg caggaatata tagatgtagg
     6001 tcaagcgaga gttcctttag cggctacttt gagtttggaa tttggtacta cctctgttaa
     6061 tatatatgct ggtatggatg atgattttaa acgttacaat gcaccaattt taacatggta
     6121 tgaaacggct cgctatgcct ttgagcgagg tatggtctgg caaaatttag gtggtgttga
     6181 aaactctctc aatggtggac tttatcattt taaggaaaaa tttaatccaa cgattgaaga
     6241 atacttgggt gaatttacaa tgcccactca tcctctctat cctctgttaa gacttgctct
     6301 tgatttccgt aaaacattaa gaaaaaaaca tagaaagtaa gtatatggca ctaacaacac
     6361 tcacgaaaga agagtttcag acttattctg atcaggtttc ttctcgttcc tttatgcaat
     6421 ctgtccagat gggggatttg ctagaaaaaa gaggggctcg aattgtttat cttgctttga
     6481 aacaagaagg agaaattcaa gttgcagctc tggtttatag cttgcccatg ctgggtggtc
     6541 tgcatatgga actcaattcg gggccaattt atacccaaca agatgctctt ccagtttttt
     6601 atgcagagtt aaaagaatat gccaagcaaa atggtgtatt agagttgctt gtaaaacctt
     6661 atgaaactta tcaaactttt gatagccaag gtaatccaat agatgctgag aaaaaaagta
     6721 ttattcaagg tttgactgat ttaggttatc aatttgatgg cttaacaaca ggttacccag
     6781 gtggagaacc agattggtta tactataaag atttaactga attaactgaa aagagtttgc
     6841 ttaaaagttt tagcaaaaag ggtaaaccct tggtgaaaaa ggctgaaacc tttggcattc
     6901 ggttgaaaaa gttaaaacgt gaagaactat cgatttttaa gaatataaca aaagaaacct
     6961 ctgaacgtag agaatatagt gataaaagtt tagaatatta tgagcatttt tatgatactt
     7021 ttggagaaca agcggagttt ctcatagcaa gcttaaattt ttcggactat atgagcaaat
     7081 tgcaaggtga acaaagtaaa ctagaagaaa acttggacaa gttgcgactt gatttgagta
     7141 aaaatcctca ttctgagaaa aaacaaaatc aactgagaga atattctagt caatttgaaa
     7201 cgtttgaagt tcgaaaagca gaagcgcgag acttgattga aaaatatgga gaagaagata
     7261 ttgttttagc tgggagttta tttgtttata tgcctcagga aacgacttat ctctttagtg
     7321 gttcctacac tgagtttaat aagttctatg cccctgccct gcttcaaaaa tatgttatgt
     7381 tggaaagcat aaaacgtgga atacctaaat acaacttcct aggcattcaa gggatttttg
     7441 atggaagtga tggtgttttg cgttttaaac agaattttaa tggctatatt gtacgcaaag
     7501 caggtacttt ccgttaccat ccatctcctt taaaatacaa agctatccag ttactcaaaa
     7561 aaatagtagg acgttaagat gaaaaagtca gtatttagat ttcttttagc ttcttttagt
     7621 aaaataattc ttatttgcta gaaaggtgga gagacatgcg ctggcttttt cgtttgatag
     7681 gggctttctt ttcttttgtg tggcgtttgt tttggcgtct ggtttggata gttgtgctct
     7741 tatgtgtgct tgctttcgga cttctctggt atctgaacgg agattttcaa ggagcgctaa
     7801 agcaagcaga acggtcagta aaaattggtc aacaaagtat tgaccaatgg gagaaaacag
     7861 ggcaactgcc taagttaagc cagacagata gtcaccagca ttctgaagga aggtgggcac
     7921 aggcctctgc tcgtatttac ctggatccgc agatggattc acgctttcaa gaggcttatt
     7981 tagaagcaat ccagaactgg aatcaaactg gtgcttttaa ctttgaactc gtgactgagt
     8041 ctagtaaggc ggatattacg gctacggaga tgaacgacgg aggcactcct gtggcaggag
     8101 aggcggaaag tcaaactaat ctcttaacag ggcaattctt gtccgtaacg gtgcggttga
     8161 atcattatta tttgtccaat ccatactatg gctactccta tgaacgcctt gtccatacgg
     8221 cagaacatga gttaggtcat gcgattggct tggaccatac agatgagaag tctgtcatgc
     8281 aaccagcagg ttccttttat ggtatccagg aagaggatgt tgcaaacctc cgaaaaatat
     8341 atgagactag tgagtagggt actatctttc cctacttttt ttgctataat ggaactatga
     8401 acaacttgat taaatcaaaa ctagagctct tgccgaccag ccctggttgc tacattcata
     8461 aggataaaaa tggcaccatt atctatgtag gaaaggctaa aaatctgcgt aatcgagtac
     8521 ggtcctattt tcgtggaagt catgatacca agacagaggc tctggtgtct gaaattgtgg
     8581 attttgaatt tattgttacg gagtctaata ttgaggcact tctcctagaa atcaacctga
     8641 tcaaggaaaa caagcccaag tacaatatca tgctcaagga tgacaagtcc tatcctttca
     8701 tcaaaatcac caatgagcgc tatccacgct tgattatcac tcgtcaggtc aaaaaggacg
     8761 gaggtcttta ttttggaccc tatcccgatg tgggggcagc caatgaaatc aagcggttgc
     8821 tggatcggat attccctttt cgtaagtgta ccaacccgcc ctctaaggtc tgtttttatt
     8881 accatatcgg tcaatgcatg gcccacacca tctgtaagaa ggatgagatt tatttcaagt
     8941 ctatggccca ggaggtttct gattttctga aagggcagga taacaaaatc attgatgagc
     9001 taaagggcaa aatggcagcg gcagcccaga ctatggagtt tgaacgtgcg gcggaatacc
     9061 gtgacctgat tcaggctatt ggaacgcttc gaaccaagca acgggtcatg gctaaggatc
     9121 tccaaaatcg cgatgtcttt ggctactatg tggataaggg ctggatgtgt gtgcaggttt
     9181 tctttgtccg tcagggtaag ctcatcgagc gcgatgtcaa tctcttcccc tacttcaatg
     9241 atccagatga ggattttttg acctatgtag gacaattcta tcaagaaaaa tcccatctag
     9301 ttcccaatga ggtactgatt ccgcaagata ttgacgaaga ggctgtcaag gccttggtgg
     9361 attccaagat tcttaagcct caacgtggag agaaaaagca gctggtcaat ttagctataa
     9421 aaaatgcccg tgttagccta gagcagaagt tcaacctgct agagaaatct gtcgagaaga
     9481 cccaaggagc tattgaaaat ctaggacgct tgcttcaaat cccaacccca gttcgcatcg
     9541 agtccttcga taactccaac atcatgggaa ctagtcctgt ttcagccatg gtggtctttg
     9601 tcaacggcaa gcctagtaag aaggattacc gtaagtataa aatcaagacc gtagtcgggc
     9661 cagacgacta tgccagtatg cgagaggtca ttcgcagacg ctatggtcga gtacagcgtg
     9721 aggctttgac tcctccagat ttgattgtga ttgatggggg gcaaggtcaa gtcaatattg
     9781 ctaagcaagt catccaagaa gaactgggct tggatattcc aattgcaggt ctgcaaaaga
     9841 atgataagca ccaaacccat gaattgctct ttggagatcc gctggaagtg gtggacttgt
     9901 ctcgcaattc tcaggaattt ttcctcctcc aacgcattca agatgaggtc caccgctttg
     9961 ccattacctt ccaccgccaa ctgcgctcca aaaattcttt ctcatctcaa ttggatggga
    10021 ttgatggtct gggacctaaa cgcaagcaaa atctcatgag gcacttcaag tctctgacca
    10081 aaatcaagga agccagtgtg gatgagattg tcgaagttgg agtaccaaga gcagtagcag
    10141 aggctgtgca aaccaagttg aacccgcagg aaacagaaat cttgcttcaa gtggcggaag
    10201 aaagagtaga ttaccaaacg gaaggaaacc acaataaacc ataaaatcgc aattttatca
    10261 gatgttcatg gcaatgcgac ggcgctagaa gcagtgattg cagatgctaa aactcaagga
    10321 gccagtgaat attggctcat gggagacatt ttcctccctg gtccaggtgc aaatgacttg
    10381 gtcgctctgt taaaggaact tcctatcacg gcaagtgttc gaggcaattg ggatgattgt
    10441 gtccttgagg ctttagatgg gcaatatggc ttagaagacc cacaggaagt tcagctcttg
    10501 cgtatgacac agtatttgat ggagcgaatg gatcctgcaa cgattgtctg gctacgaagc
    10561 ttgcctttgc tggaaaagaa agaaattgac ggattgcgct tttctatctc tcataattta
    10621 cctgacaaaa actatggtgg tgacttgcta gttgagaatg atacagagaa atttgaccaa
    10681 ctgctagatg cggaaacgga cgtggcagtt tatggtcatg ttcacaagta gttgcttcgt
    10741 tatggtagtc aagggcaaca aatcatcaat ccagggtcga ttggcatgcc ctattttaat
    10801 tgggaggcgt taaaaaatca ccgtgcccag tatgccgtga tagaggttga agatggggaa
    10861 ttggtaaata tcctatttcg taaagtcgct tatgattacg aggctgagtt agaatttgcc
    10921 aaatccaagg gttttccctt tatcgaaatg tatgaagaac tacgtcgtga agataactat
    10981 cagaggcaca atctggaact attagcaagc ttaatagaaa agcataggta tgtagaggat
    11041 gtgaagaatt tttttgattt tttgtaagag tttcctaaaa taaccaatgc aaactaaaaa
    11101 agcgattggc tgatcccatc gcttttaata tatcttatac tcaatatgaa aatcaaagag
    11161 caaactagga agctagccgt aggttgctca aagcacagct ttgaggttgc agataaagct
    11221 gacgtggttt gaagagattt tcgaagagtg ttattgtaac tgagattgat ctgggaggta
    11281 agaaccacct agataggtat tgctgagttt ttcaagggtt ccgtcttgat agagttcttt
    11341 gagcgcttta tcaaattgct ctttaaactc tttttggtcg cttgagaaaa tgatataatt
    11401 gctggggcta tctgcagaag gtaaatcaac gactgagagg tctaaaccac ggtccttgat
    11461 aatcttttga acggatacct tgtcaaaaac taggaaatca aactctccgt tagcaaggtc
    11521 taggattcgt ttaccaatat cctcaccaga aaaattaatt gtagcgggat tatcagtgtg
    11581 tttctgattc cagttattga tgaattgagc gttagaagtt ccggtatcct cttgtgttgt
    11641 tttaccagcg atctggtcaa gagaagtcaa aggatttttc ttgttgctga caaggacgag
    11701 gggattgttg gaaatcggaa gcgagtaaag gtatttttca gcacgctctt ttgtgtaact
    11761 caaattattg gccgcagcct gatagtgacc agaatcaagt cctgggaaga tactttccca
    11821 ggcggttctt tgaaattgaa tctcgtagtc gctgagtttt tcatctactg cctttagaac
    11881 ttcgatatcg aagcctgtca gattgccttt atcttcgtag tcaaatggtg gcacgtcgcc
    11941 agctgtagca aggacgattg tcttttgagc gctagtctct ttgggtgtag cttgattccc
    12001 acaggcaacc aaaaatggta ggatagctag taataggcta aattttttca tactgtctcc
    12061 attcaaatgt aaagtacttg ctagtttatc agaaactttc ctgataaacc aatatatatc
    12121 ttttatgtct gtgatagaaa aacttaatca tgaaaaatcc ctgcaagctc tttcaaatta
    12181 tggtagaatg gaagcagtag aattagaaaa ggaaatcgat tatgaaattt cttgaattaa
    12241 ataaaaaacg tcatgcgacc aagcatttca ctgataagcc tgttgatcca aaagatgtgc
    12301 gtacggctat cgaaattgca accttggcgc caagcgccca caacagccag ccttggaaat
    12361 ttgtagttgt tcgtgagaaa aatgctgaac tggcaaagtt ggcttatggt tcaaactttg
    12421 agcaggtatc atcagcacct gtaaccattg ccctatttac agatacagac cttgccaaac
    12481 gcgctcgtaa gattgcccga gttggtggtg ctaacaattt ctcagaagaa caacttcaat
    12541 acttcatgaa aaatttgcca gctgaatttg cacgctacaa tgaacaacaa gtcagcgact
    12601 acctagctct caatgcaggt ttggttgcca tgaacttggt tcttgcattg acagaccaag
    12661 gaattggttc taacattatt cttggttttg acaaatcaaa agttaatgaa gttttggaaa
    12721 tcgaagaccg tttccgccca gaactcttga tcacagtggg ttatacagac gaaaaattgg
    12781 aaccaagcta ccgcttgcca gtagatgaaa tcatcgagaa aagatagaaa gaagaaaaaa
    12841 tgacagcaat tgattttaca gcagaagtag aaaaacgcaa agaagacctc ttggctgact
    12901 tgtttagcct tttggaaatc aattcagaac gtgatgacag caaggctgat gcccagcatc
    12961 catttgggcc tggtccagta aaagccttgg agaaattcct tgaaatcgca gaccgcgatg
    13021 gctacccaac taagaatgtt gataactatg caggacattt tgagtttggt gatggagaag
    13081 aagttctcgg aatctttgcc catatggatg tggtgcctgc tggtagcggt tgggacacag
    13141 acccttacac accaactatc aaagatggtc gcctttatgc gcgcggggct tcggacgata
    13201 agggtcctac aacagcttgt tactatggtt tgaaaatcat caaagaattg ggtcttccaa
    13261 cttctaagaa agttcgcttc atcgttggaa cagacgaaga atcaggctgg gcagacatgg
    13321 actactactt tgagcacgta ggacttgcca aaccagattt cggtttctca ccagatgctg
    13381 aatttccaat catcaatggt gaaaaaggaa atatcacgga atacctccac tttgcaggag
    13441 aaaatacagg tgttgcccgt cttcacagct ttacaggtgg tttacgtgaa aatatggtac
    13501 cagaatcagc aacagcagtc gtttcaggtg acttggctga cttgcaagct aaactagatg
    13561 cctttgttgc agaacacaaa cttagaggag aactccaaga agaagctggc aaatacaagg
    13621 tgacgatcat tggtaaatca gcccacggtg ctatgcctgc ttcaggtgtc aatggcgcaa
    13681 cttaccttgc cctcttcctc agtcagtttg gctttgctgg tccagccaaa gactaccttg
    13741 acatcgcagg taaaattctc ttgaacgatc atgagggtga aaatcttaag attgctcatg
    13801 tggatgaaaa gatgggtgct ctttctatga atgccggcgt cttccgcttc gatgaaacaa
    13861 gtgctgataa taccattgcc ctcaacatcc gctatccaaa aggaacaagt ccagaacaaa
    13921 tcaagtcaat ccttgaaaac ttgccagttg tttctgttag cctgtctgaa cacggtcaca
    13981 cgcctcacta tgtgccaatg gaagatccac ttgtgcaaac cttgttgaat atctatgaaa
    14041 aacaaactgg ctttaaaggt catgaacaag tcatcggtgg tggaaccttt ggtcgcttgt
    14101 tagagcgcgg agttgcctat ggtgctatgt tcccagactc aattgatacc atgcaccaag
    14161 ccaatgaatt tatcgccttg gacgatctct tccgagcagc agcaatctat gccgaagcta
    14221 tttacgaatt gatcaaataa aacgatagaa gtctgagatc ttatgcttgg acttcttttt
    14281 ggagggaaag tagatgtctc aaatcgaaag aatcaaacag gctatcatgg cggattcaca
    14341 gaatgccagc tatacagagc gtggcattga gcctctcttt gcagcgccaa aaactgctcg
    14401 catcaatatc atcggtcagg ctccgggact taaaactcaa gaagcaggcc tttactggaa
    14461 agataaaagt ggtgaccgct tgcgggactg gctaggtgtg gatgaagata ccttttacaa
    14521 ttcaggttat tttgctgttt tgcctatgga tttctacttt ccaggacatg gcaagtcggg
    14581 tgatcttccg cctcgtacag gttttgcaga aaaatggcat ccgcagctct tacaggaatt
    14641 gcctgatatt cagttaaccc tcttgattgg gcaatatgcc caagcctact atttacagga
    14701 gaaaatcagt gggaaggtaa cggagagggt gaaacactat aaagactatc tgccagccta
    14761 ttttccgcta gttcacccat caccacgaaa tcaaatctgg atggccaaaa atccttggtt
    14821 tgaggcagaa gtagtgccag atttgaaaaa aagaattaaa accattttat agtcaatgaa
    14881 aatcaaagag caaactagga agctagtcgt aggctgctca aagtacagct ttgaggttgc
    14941 agataaaact gacgaagtcg gtaacatacg cacggtaagg cgacgctgac gtggtttgaa
    15001 gagattttcg aagagtatta gaagaaaaag aatgaaagaa atagcctttg acgcatttta
    15061 ccagctttac caaaacgacc agctttctct agtggatgtg agagaagtgg atgagtttgc
    15121 agctcttcat ttagaaggtg cccacaacct accgcttagt caattggctg atagttatga
    15181 ttaattggac aaagatcgct tgcattatat tatttgcaaa tctggaatga gatcggcgcg
    15241 tgcttgccaa ttcctgttag aacaaggtta taatgttatc aatgtccagg gtggcatgtt
    15301 agcctttgaa gaactttaaa attttgcatt tctcctactt ggtgtggact gggtaggaga
    15361 gttttatttt tagataattc ttatttttaa gaaaattgaa aacgtttaat atttgcctcg
    15421 tgatgctttt ttcagactcc taatcgtggt atactaggtc agtattttat aaatatgaag
    15481 gagattttta tggctaaaaa aggtacccta acaggtttgc tcctgtttgg aatatttttt
    15541 ggtgcgggga acttgatttt tccgccttct ctaggtgctc tatctggaga acattttctt
    15601 cctgccatcg caggttttgt cttttcaggc gttggtatcg ccgtcttgac ccttattatt
    15661 ggaacgctaa atcctaaagg atatatctac gagatttcaa cgaagatagc gccttggttt
    15721 gcgactcttt acctctcagt tctttacttg tcaatcggtc cattctttgc taccccacgt
    15781 actgctacaa cagcttacga agtagggatt agcccccttt tgtcggatgc aaataaagga
    15841 cttggcttga ttgtatttac ggttctgtat tttgcggcag cctatttgat ttcgcttaat
    15901 ccatcaaaaa tcttagaccg cattggacgt gttttaacgc cagtctttgc aattttgatt
    15961 gttatcttgg tcgttctggg agctatcaaa tatggtggaa caagtcctca agctgcttca
    16021 gctgcttatc aagcttctgc ctttggtaca ggtttcctag aaggttacaa taccttggac
    16081 gcccttgctt cagtggcctt tagcgtaatc gcagttcaaa ccttgaaaca acttggattt
    16141 tcaagtaaga aagaatacat ttcaactatt tgggttgttg gtatcgttgt tgcccttgcc
    16201 ttcagcgctc tttacatcgg tttaggtttt cttggaaatc atttcccagt accagctgaa
    16261 gcgatgaagg gtggaacacc aggtgtttac atcttgtcac aagccactca agaaatcttt
    16321 ggctcaacag ctcaactctt ccttgcagct atggttaccg taacctgctt cacaacgact
    16381 gttggtttga ttgtgtcaac agctgagttc tttaatgagc gcttcccaca aatcagctac
    16441 aaggtttatg cgacagcctt taccttgatt ggatttgcta ttgccaattt gggtcttgat
    16501 gcgattatca agtactcaat tccagtactg gttatcttgt acccaatcac gattgctatc
    16561 gttatgattg tcattgtcaa caaatttgtt gccctttcaa aaccaggtat gcagttgaca
    16621 attgctgtgg ttacagttat tgccattgca agcgtactag gaagctcgtt taaggttgag
    16681 tttcttgcaa atcttgttag cgttcttcct tttgccaagg catctctccc atggttggtg
    16741 ccagccattg ttggaatctt gctctcattg gttctaccaa acaagcaaga aagcgatgtt
    16801 tttgaaatgg aataatcact taaatcactt ttgtagccaa gtctacagga gtgattttct
    16861 ttttttatcc gatgataaat gtgttataat aggtagcgaa agaggtgaag aaatgaatca
    16921 aacagtagaa tatatcaaag aactgacagc catcgcatcg ccaacgggct tcactcgtga
    16981 aatttcgaat tatttagtca agactctaga aggttttggt taccagccag ttcgcacagc
    17041 taagggtggt gtcaatgtga ccatcaaagg tcaaaatgat gagcaacatc gctatgtgac
    17101 tgcccatgtg gatacgctgg gtgctattgt tcgtgctgtc aaaccagatg gtcgtcttaa
    17161 attggaccgt atcggtggtt ttccttggaa catgattgaa ggtgaaaact gtaccgttca
    17221 tgtggctagc acaggtcaaa aggtatcagg aaccatcctc atccaccaaa cttcttgcca
    17281 tgtctacaag gatgcaggaa ctgcagaacg cacgcaggac aatatggaag tgcgtttgga
    17341 tgtaaaagta agcaatgaaa aagaaactcg tgctcttggg attgaggtcg gtgattttat
    17401 cagcttcgat acacgaactg ttgtgactga tacaggtttt atcaagtctc gtcatttgga
    17461 tgataaggtc agcgcagcga ttttgctcaa cctgcttcgt atttataaga aagagaagat
    17521 tgaattgcca gtaacaactc attttgcttt ttcagttttt gaagaagtag gacatggtgc
    17581 taattctaac attcctgctc aggtagtaga atatctggct gtggatatgg gagctatggg
    17641 agatgaccag cagacagatg agtacacagt atctatctgt gtcaaggatg cttcaggtcc
    17701 ttatcactat gacttccgtc aacacttggt tgccttggct aaagagcaag atattccatt
    17761 taagctcgac atctatccat tttacggatc ggacgcttca gcagcgatgt cagcaggtgc
    17821 agaggtcaag catgcacttc ttggagctgg tattgagtcc agtcactctt atgagcgcac
    17881 ccacatcgat tcagtaatag cgactgagcg tatggttgat gcttatctta agagcgaggc
    17941 ggtatcatag aatgtgctta atctgtcaga gaatcgaact cattaaagct gggcaaaatc
    18001 cctactttgt aaaagaacta gaaacaggct atgttgttat tggtgactac caatacttta
    18061 agggctatac cttattttta gcaaaagatc atgtcacaga actccatcat atggaaactt
    18121 ctgtaaaact tcgcttttta gaggaaatga gtttagtcca agaagcagtc gccaaagcat
    18181 ttgaggctga aaagatgaat attgaactcc taggaaatgg cgatgcccac gctcattggc
    18241 atctttttcc ccgaagagca ggtgatatga agagtcacgg tttgaatggc cgtggcccag
    18301 tctggtgggt gccctgggaa gaaatggcgg cagaagattg ccaagtacaa tcgccagagc
    18361 tggaagagat gattaaaatc ttatctcatg aattggagaa gtatttggcc taagagagag
    18421 gaagaaagaa atgaaaaaaa gatatgtcat tttatctggt ttactagcat tgacccttgc
    18481 agcttgttcg caagaaaaaa ccaaagttga agaaaacact caaaaaacgg agcaaagcag
    18541 tcaaccggaa ggaactgtag gtagcaaatc tcaagcttct agtcagaaaa aggcagaagt
    18601 tagcaataaa gggagctatt atagtatcca agggaaatac gatgagatta tcctagctaa
    18661 taaacgttat cctttgtcaa aagattacaa tccaggagaa aatccaacag ccaaggcaga
    18721 gttgctcaaa ctaatcgcag ctatgcaggc tgagggctat ccaatcagtg accaatacag
    18781 tgggtttagg agttatgaaa ctcaggctaa gctctaccaa gactatgtca atcaagatgg
    18841 taaggaagca gcagatcgct actcagcacg cccaggttac agtgaacacc agacgggtct
    18901 tgcttttgat ttgattggga cagatgggga tttagttact gaagaaaagg cagcccagtg
    18961 gctcttggac catgcggctg actatggctt tgttgttcgc tatcttaaag gcaaggaaaa
    19021 ggagactggc tacatggcag aagaatggca tctccgctac gttggaaaag aagccaaaga
    19081 aattgctgca agtggtctca gtttggaaga gtactatggc tttgaaggcg gcgattatgt
    19141 cgattaaaaa tgaaagtttt ctcttgcatt tttagataaa tagtgtataa tggaaaggta
    19201 tgtgtaaagc atacttgtgg gaggtaaaaa tctctaatta ccgccaaaac cacaaaggag
    19261 gatttaaaaa tggctaaaaa agtcgaaaaa cttgtaaaat tgcaaatccc tgctggtaaa
    19321 gctacaccag ctccaccggt tggacctgct cttggtcaag ctggtatcaa catcatggga
    19381 ttcacaaaag agttcaacgc tcgtacagct gaccaagctg gtatgatcat tccagttgtt
    19441 atctcagttt acgaagataa atcatttact ttcgttacga aaacaccacc agctgctgtt
    19501 cttttgaaaa aagctgcagg tgttgaaaaa ggatcaggta cacctaataa aactaaagtt
    19561 gctacagtta ctcgtgcaca agtacaagaa attgcagaaa ctaagatgcc agatttgaac
    19621 gcagcaaacg tagagtctgc aatgcgtatg atcgaaggta ctgctcgttc tatgggattc
    19681 actgttgttg actaatcaat aacacagtag aaaatctcac agcagtctat tagttgcttt
    19741 tcatactagg caagtgactg aggcttgtac ttggatacag caagggagct taaggccgta
    19801 ggaagagaaa aatagtagac tgaaaacccg caagacttca tcatttcgag aagtgacgtg
    19861 ggagatgaaa atcgattgaa ccacttacaa ggagaataga aaatggctaa aaaaagcaaa
    19921 caacttcgtg ctgctcttga gaaaatcgac agcacaaaag catacagtgt agaagaagct
    19981 gtagcacttg caaaagaaac taactttgca aaatttgatg caactgtaga agttgcttac
    20041 aacttgaaca tcgacgttaa aaaagctgac caacaaatcc gtggagcaat ggtattgcca
    20101 aacggtactg gtaaaacttc acgtgttctt gttttcgcac gtggtgcaaa agctgaagaa
    20161 gcaaaagctg ctggtgcaga ctttgttggt gaagatgacc ttgttgctaa aatcaacgac
    20221 ggttggttgg acttcgacgt agttatcgct acacctgata tgatggctct tgttggacgt
    20281 cttggacgtg tccttggacc acgtaacttg atgccaaacc ctaaaactgg tactgtaaca
    20341 atggatgttg ctaaagcagt tgaagagtct aaaggtggta aaatcactta ccgtgctgac
    20401 cgtgcaggta acgttcaagc aatcatcggt aaagtatcat ttgaagctga aaaattggtt
    20461 gaaaacttta aagctttcaa cgaaacaatc caaaaagtaa aaccagctac agctaaagga
    20521 acttacgtaa caaacttgac tatcacaact actcaaggtg ttggtatcaa agttgacgta
    20581 aactcacttt aatcagtagt taaagtaatg taaaaaagtt gaagacacta tgtctcaact
    20641 ttttttgatg tacgacgggc atgtcgtata tctgaggtgt aagtcctcgg tgggcaccta
    20701 ctaccggtga acccaatagc gattcccaag tctgacgctg gaaataagaa tcgtcagagg
    20761 aagggatagc gaaatcgtgg ctctacgaac aggaacgtga taataaggcc tatatagcgg
    20821 ataaggtatc tggctggggc gaccactatc aattataata aaatgaaata agaataggac
    20881 gaatcgttca ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat
    20941 tctagtttca atctactata gtagctcaga agtcggtact taaacgtgct atatcaaaac
    21001 cagtccttga aaaacgtgga ctggtttcgt gtttggatta ttaccttgaa cgacatgcgt
    21061 taaaagttag ttgaaccgcc gtatgccgaa cgg
//
LOCUS       DAVKHZ010000033        21044 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000033 DAVKHZ010000000
VERSION     DAVKHZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.033"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(187..537)
                     /locus_tag="VWW99_001112"
     CDS             complement(187..537)
                     /locus_tag="VWW99_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538964.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(677..1012)
                     /locus_tag="VWW99_001113"
     CDS             complement(677..1012)
                     /locus_tag="VWW99_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538965.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1100..2410)
                     /gene="der"
                     /locus_tag="VWW99_001114"
     CDS             complement(1100..2410)
                     /gene="der"
                     /locus_tag="VWW99_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9538966.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2424..3137)
                     /locus_tag="VWW99_001115"
     CDS             complement(2424..3137)
                     /locus_tag="VWW99_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9538967.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3134..4030)
                     /gene="dnaI"
                     /locus_tag="VWW99_001116"
     CDS             complement(3134..4030)
                     /gene="dnaI"
                     /locus_tag="VWW99_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9538968.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(4031..5200)
                     /locus_tag="VWW99_001117"
     CDS             complement(4031..5200)
                     /locus_tag="VWW99_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9538969.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5201..5674)
                     /gene="nrdR"
                     /locus_tag="VWW99_001118"
     CDS             complement(5201..5674)
                     /gene="nrdR"
                     /locus_tag="VWW99_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9538970.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5814..6179
                     /locus_tag="VWW99_001119"
     CDS             5814..6179
                     /locus_tag="VWW99_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9538971.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6184..6879
                     /locus_tag="VWW99_001120"
     CDS             6184..6879
                     /locus_tag="VWW99_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538972.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6896..7663
                     /locus_tag="VWW99_001121"
     CDS             6896..7663
                     /locus_tag="VWW99_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538973.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(8016..9215)
                     /locus_tag="VWW99_001122"
     CDS             complement(8016..9215)
                     /locus_tag="VWW99_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9538974.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFLLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9212..10105)
                     /locus_tag="VWW99_001123"
     CDS             complement(9212..10105)
                     /locus_tag="VWW99_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9538975.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10221..10301
                     /locus_tag="VWW99_001124"
                     /pseudo
     CDS             10221..10301
                     /locus_tag="VWW99_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10811..10999
                     /locus_tag="VWW99_001125"
                     /pseudo
     CDS             10811..10999
                     /locus_tag="VWW99_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11414..12031
                     /locus_tag="VWW99_001126"
     CDS             11414..12031
                     /locus_tag="VWW99_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9538976.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(12351..13253)
                     /locus_tag="VWW99_001127"
     CDS             complement(12351..13253)
                     /locus_tag="VWW99_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9538977.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHP
                     EMGHYYVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDV
                     QAYYIAQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYI
                     VTPAVAGNGSATLGNFVLAKEVSK"
     gene            complement(13402..15285)
                     /locus_tag="VWW99_001128"
     CDS             complement(13402..15285)
                     /locus_tag="VWW99_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9538978.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            15662..17116
                     /locus_tag="VWW99_001129"
     CDS             15662..17116
                     /locus_tag="VWW99_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9538979.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWVQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAINNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRVLAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNSQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            17097..18062
                     /locus_tag="VWW99_001130"
     CDS             17097..18062
                     /locus_tag="VWW99_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9538980.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTQEKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(18098..19372)
                     /locus_tag="VWW99_001131"
     CDS             complement(18098..19372)
                     /locus_tag="VWW99_001131"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9538981.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMAILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(19372..20544)
                     /locus_tag="VWW99_001132"
     CDS             complement(19372..20544)
                     /locus_tag="VWW99_001132"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9538982.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(20675..20923)
                     /locus_tag="VWW99_001133"
     CDS             complement(20675..20923)
                     /locus_tag="VWW99_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538983.1"
                     /translation="MVSSSSSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
BASE COUNT         6155 a         4644 c         3981 g         6264 t
ORIGIN      
        1 tgtaatagaa tgtgaacgaa tcaattaggg gatctaaagc attttatacc aatgttttat
       61 aagttgtatt gtattattcc atattcaatc cactatacaa gatgaatatt tctctcaagt
      121 ttcataatca aaataaaaat gactgtatct atagctccat aaacaaatca tagatacagt
      181 cattttttag ttcctacacc ttcctttatg cacaccttta gatttcccta gttcccgcct
      241 caagcgaatc atttttttga aaaagacata ggctaaccaa tcactagcaa aagcgataag
      301 tagtaataat agccaaatac cccataactt gatatctgtc aaatttctca agacgatatt
      361 gaaaaacaga actgaaaaaa ctgtccaagc aaggctaaaa agagcataga gggtgatgta
      421 aatccagtaa aaatagtaaa aaattgggaa aaatttactt tctctattat ccttgtcaat
      481 ccagctgaaa aagtaaaaac agggtaataa gattatcatt ttaaacaagt gttccatcac
      541 aagcctcctt ttttaaattg ttttctttag tttcgttcta ttaaatgtta ttaccaatat
      601 aaaccttgga atagcgtgta ataaatgatt ttagaggtac cataagccac ctcctacaaa
      661 tagaaaccga tataaatcaa tgccttccac ccttagactt ccctagttcc tgtctcaagc
      721 gaaacatttc tttgaaacag gaataagtta accaattcat accaatagct agcagaataa
      781 aaagaaacca aatgccccat aacttgatat ctgtcacatt tctcaagacg gtattgaaaa
      841 acagaactga aacaactgtc caagcaaggc taaaaagaga atagaagggg atgtaaaacc
      901 agtaaaaata ataaaaaatt ggaaaaaact tactatttct gttggccttt tcaatccagt
      961 tatcaaaata aaagtacggt gctaaaagta agaatttaaa caaatgttcc atcaccgaca
     1021 tcccccttct tttgatagcg ttttctatta ttttattata tcaaaaaaat ccggaactgt
     1081 cattccagat tctacttttt tatttgcgtt ttcttgcgat gagatgaatc ggtgttccct
     1141 caaaaacaaa ggccttgcgg atttgatttt ccaagaaacg caggtaagaa aagtgcatga
     1201 gttcttcttc attgacaaag atgacaaagg ttggtggttt ggttgccact tgggtcgcat
     1261 agaaaatctt gagacgtttt cctttgtctg tcggtgttgg gttgatggca atggcatcca
     1321 tgatgacatc gttcaagaca gctgatggaa tacgtgtatt ttgactttcg ctgatttgct
     1381 taatcatctc aggaagtttg tggagacgtt gcttggtcaa agcggataca aagataatcg
     1441 gtgcgtaagg caggtattgg aactgctcac ggatatcttc ttcccagttt ttcatagtgt
     1501 ggttatcttt ttcaagcgta tcccacttgt tgaccacgat aatcatccct ttaccagctt
     1561 catgagcaaa tccagcgata cgcttgtcgt attcacgaat cccctcttcc gcattgatga
     1621 ccattaagac cacatctgaa cggtcaatag cacgcatggc acgcatgaca gagtacttct
     1681 cagtattttc ataaacctta ccagacttac gcataccagc cgtatcaatc atggtaaact
     1741 cttgaccatc tgtatctgta aagtgggtat caatagcgtc acgagttgtt ccagcaacag
     1801 gactggcaat gacacggtct tctcccaaga tagcattaat caagcttgat tttccaacgt
     1861 taggacgacc aatcaagcta aacttaatga catctggatt ttcttcttca tattcatttg
     1921 gaaggttttc cacaatcgca tctagtacat cccctgtacc gataccatgg acagatgaga
     1981 taggcagtgg ttcacccaaa ccgagagcat agaaatcata gatatcattt ctcatctcag
     2041 ggttgtccac cttgttgact gcgaggataa ctggtttgtg ggtcttataa agcttacgag
     2101 ctacgtattc gtctgcatca gtaattcctt ccttaccaga cacgacaaaa acgataacat
     2161 ctgcttcttc catggcaatt tctgcctggt gcttgatttg ttccatgaaa ggagcatcga
     2221 catcatcaat tcctcctgta tcaatcatgc taaaagaacg attgagccac tcacccgttg
     2281 cataaatacg gtcacgtgtc actccttcga catcttctac aatggagatt cgctcaccag
     2341 cgatccgatt aaatagggtt gatttcccaa cattgggacg tcctacaatg gcaatagttg
     2401 gtagggccat aatttctcac tttctacaat aatttcttct gttcaagatt ttttctagtt
     2461 gagcttggtt cagcttgacc aaactgttct gctaggcgct gactccagct tgtggtcgca
     2521 cgcgccccag catagtcagc ctgaacacgg tcataagctt ggattgcctc agttgactgt
     2581 tcttggtatt cttcctcaaa gacaacattc tctagtggca gtctcggttt catatcatga
     2641 tgttgatttg gcacacccag tgccatccca aagacagaat aggtgtagtc aggtaggtta
     2701 aagagctctg ccacttcttc agacttgtat cgaaccaaac cgataatcac accaccatag
     2761 cccaagcttt cagctgccaa caaggcgttt tgtccagcaa gagctgcatc gaccgaacta
     2821 atcaagagac cttccacacc ttggggttgg aaggtgtcgg tatgaagtct ggctcccttt
     2881 tctgctcggt tcaaatctcc gacaaagaga aggaaaacag cagactggcg aatggcttct
     2941 tgaggtacca attcatacaa ggcatctttc ttctcttgac ttcgtaccac aatcacagag
     3001 taggattgga aattcttcca agatgatgcc atctgggctg ctgtcaaaat ctcatttaag
     3061 tctacttggg gaatttcttg ctctttaaac ctgcgcactg aagtatgagc cttcatcaat
     3121 ttaatggttt ctgtcatcga cggtttactc cttctaaacg agtctcctca gccaaataac
     3181 ggatgcgttc catgacccgt ctggcttccc aggtttcgtc atttccatgt ttcactttcg
     3241 caaaatgctt ctccaaatct tcaaagttga agttggatgt gaaaaaggtc ggtaaatttt
     3301 cctgcatccg atattggaga atgacctgca ggatttcatc acgcacccaa gcggtggatt
     3361 gctcggcgcc aatatcatct aaaatcagga cctcagacag cttaatctca tccaccaagg
     3421 tcttaacatt gccatcactg atagcatttt tgacatcaat gacaaagcta ggatagtgga
     3481 ggagagttga tgaaacacca cgtttttctg ataaatcatg agctaaggcc gccaccatga
     3541 aacttttacc cacaccaaag tctccatata agtaaagacc ttttcgaata gctggatatt
     3601 gctccacgaa ggctagtagc ttttcaaaaa ctggtaagcg ccccaaatca tccaagtcaa
     3661 cttgagccaa actagctttc ttgagactgg ctggtagatt gattaacttg agacggttct
     3721 taatagccgc ttctttttca gccgcgatta gctcaggagt ttcttcatat gaaacatctg
     3781 catagccatg attcttaacc aaaatcggct tgtagccttt ggcaatataa tccgtatccc
     3841 cacggagaaa cttgtcacgc tcggtgatgt actgattaaa cttggagata ctgcgattta
     3901 attcctctgg agtcaaggat tcttgctgga taaaagccgc aacatcaggg tccttcatga
     3961 ttttctggac caaatcttga taataaaaac ggctgggttg acgtttgagt acgtctccga
     4021 cactttccat ctaatctcct cctttttcta atcgagctaa tagttcttgc ttcttacgtt
     4081 ctagttccag acgagtttcc tcgctggttt cattcttata ttcaggatta ctccatttag
     4141 gaacattggt ttttactggg gcagtctgat tctgtttttg tgtttttgct ttctgtcctc
     4201 gctcacggat gcgcaagact gccttttctg ccgaatgaat cttttgatag gcatagtcat
     4261 tggctacctt catggcatat ttctcattga tatttgccga atcgacctta ttaaaggtca
     4321 ataaaagaat gatattgatg acttcgtcca gcaagcccaa gccagccatc tgttgcaaga
     4381 gttctctttc tgtttgggta atggttccct tgcgtgtttg cttgatttct gccaagaact
     4441 gcagggcagt tttacttttg gcttctttga taatggttgt ttccttaaga ctaaagtcag
     4501 aggaaactgg tttttgagca attttttcac gcatgcgttt ggttgaaata acctgagaaa
     4561 cagctgttga cttggccaat tgataggttt caaaccaagt ccatttcttc tcctcggcaa
     4621 tagcaaagag gtttaagaca tcggactgct catccgcaaa acgaagtcca tctcgagcca
     4681 tcagctggcg aaaatgttcc aagtcaaaat cattggccac tttcttcttg agaccaaggt
     4741 cttcttgact gcctagttct gccaattctg gaaagacttg attgagtgag acaggtattt
     4801 cttcaccatc agcactttca actttcaaat cctccacagc agagtcgcca atctttttct
     4861 ctaagagtct gcgataaaca ggatgctcca agaagtcttg actagataga ggagcatgga
     4921 gggctagctg ataaacatcc cccttttgat agagggtcaa gagattaaaa gcagataaga
     4981 ttttcaatga ttttatcagt ctatccatcc caaagttgag atgattgagg atacttgaaa
     5041 aaagatattc ctttctacca ttatcccaaa aactgattgt ataaagataa aggctcagtg
     5101 cctcctgacc gataatcggg aggtagcact gtaccagaga tgaggtatct tgcgacaccc
     5161 gattattctt tagataagaa aaacggtcaa ttggcttcat ttatctttcc tttttctttt
     5221 tagaggactg ggtgatttgt tggagcaagc tctctaactc actgacatcc ttaaaactac
     5281 gatagacact agcaaaacgt acataggtaa tctcgtccaa ttcagccaac tcctccatga
     5341 cgagtgaacc aatgtcctca ctttgaattt cattttcatt tcgaccacgg agtttctgtt
     5401 cgatacgatt gactaccatg ttgatttcat cacttgacac aggacgtttc tgggctgagc
     5461 ggataatccc attaaagatt ttatctctgg agaattgttc ccgtgtacca tcttttttaa
     5521 caaccactaa ggttctttct tctactcgtt cgtaggttgt aaaacggtgt tggcattcgt
     5581 cgcactcacg tcttctacga atggtgttcc cttcttctgc ttggcgacta tcgataacac
     5641 ttgacttggt agccccacat tttggacaac gcatcctttc cctccttatc gttttctttt
     5701 cattatacca ttttttaagc gattcccaaa acaattcttc tttttgcttg acaagttttt
     5761 tgttttgttg tattatttaa ttaagacaaa aggataagag aaaggagact aagatgtcct
     5821 ggacatttga caacaaaaaa cccatctatt tacagattat ggagaaaatc aagcttcaga
     5881 ttgtttccca tacactggaa cccaatcaac aacttccaac cgtgagggag ctagctagcg
     5941 aggctggtgt caatcccaat accatccaaa gagccttatc agaccttgaa cgagaaggat
     6001 ttgtctacag caagcgaaca actggacgat ttgtgactaa ggataaggag ctaatcgccc
     6061 agtcacgcaa acaattatca gaagaagaat tggaacactt cgtttcctcc atgacccatt
     6121 ttggctatga aaaagaagaa ctaccaggcg tagtcagtga ttatattaaa ggagtttaag
     6181 cctatgtcat tactagtatt tgaaaatgta tccaaatcat atggagcaac accagccctt
     6241 gaaaatgttt ctcttgacat tccagctgga aaaattgtcg gccttcttgg gccaaacggc
     6301 tcaggaaaaa caaccctgat taaactaatt aatggcctct tacaaccaga tcaaggacgt
     6361 gtcctcatca acgacatgga cccaagccca gcaaccaagg ccgttgtagc ttatttgcct
     6421 gatacgacct atctcaatga gcaaatgaag gtcaaagaag ccctaaccta cttcaagacc
     6481 ttctataaag atttcaatct tgaacgcgcc catcatctac ttgcagacct gggcattgat
     6541 gaaaatagtc gtctcaagaa actatcaaaa ggaaacaaag aaaaggttca actgattttg
     6601 gttatgagcc gtgatgctcg tctctatgtt ttggacgaac ccattggtgg ggtggatcca
     6661 gcagcccgtg cttatatcct caataccatt atcaacaact actcaccaac ttctaccgtt
     6721 ttgatttcta cccacttgat ttctgatatc gagccaatct tggatgaaat tgtcttccta
     6781 aaagacggaa aagtcgtccg tcaaggaaat gtagatgata ttcgctacga gtcaggtgaa
     6841 tccattgacc aactcttccg tcaggaattt aaggcctaag caaaggagat tatttatgtt
     6901 ttggaattta gttcgctacg aatttaaaaa tgttaacaag tggtatttag ccctctacgc
     6961 agccgtgcta gtcctttctg ccctcatcgg aatacagaca caaggcttta aaaatctacc
     7021 ttaccaagaa agtcaggcta ctatgctact ttttctagct acagtctttg gtggcttgat
     7081 gcttacactt gggatttcaa ccattttctt gattattaaa cgcttcaaag gtagtgtcta
     7141 cgaccgacaa ggctatctga ctttgacctt gccagtttct gaacaccata tcatcacagc
     7201 caaactaatc ggtgccttta tctggtcatt gattagcacc gctgtattgg ctctaagtgc
     7261 tgttattatt ctggctttaa cagctccaga atggattcct ctttcttatg tgattacatt
     7321 tgtagaaaca catctccctc agatctttct tacaggtata tccttcctac taaatactat
     7381 ttcaggaatc ctctgcatct acctggctat ttccattgga cagcttttca atgaataccg
     7441 tacagcactc gctgttgcag tctacattgg tatccaaatc gtcattggat ttattgaact
     7501 tttcttcaat cttagttcta atttctatgt caattcactg gtaggactca atgaccattt
     7561 ctatatggga gcaggtatag ccattgttga agaactcata ttcatagcta tcttttatct
     7621 cggaacctac tacatcttga gaaataaggt taatttgctt taaataattt ttacctagat
     7681 atgtaacata ctcatagaac aaaagagacc aggcaaaaag tctttacaat tagaaaacgc
     7741 atagtatcaa gtgttaaaaa tccttgatat tatgcgtttt ttgtgagctg acttatttcc
     7801 tttcactata tcgcaaaatg aaataagaac ggaacgatgg gattttggaa ttcaaatcaa
     7861 tttataagaa tgttttagaa gtaatattat cctattccag attcagttca ctatacaatt
     7921 gagttttcaa gcaacctgtt tacataatgt gtacataatt aggttcgtga ttccaccctt
     7981 ttcaccttta aaaacctcgc tttcgcaagg ctcttctatt tataagataa ggcacgttta
     8041 aaggttttcc aaatccctaa atcatccgtt tgaagaacga gactagcata catgcgtccg
     8101 ataaatcctg ttgctaccac cgcaaaaatc actgtaatag caagtgaaat ccatgcttcc
     8161 gctccctccg catagccatt aatcgttcga aacggcataa agaaggtcga aataaaggga
     8221 atataagaac caatcttcaa gaggagattg tcaccagctg cacctagagc tgtcactcca
     8281 aaaaaaccac ccataatcaa aatcatcaaa ggcgacaagg ctttccctga gtcttcagga
     8341 cgagaaacca tagatcctag gaaggctgcc aagactacgt acatgaaaag actgatcaaa
     8401 ataaagagca aggtattcag tgagatagca tctcccaagt gatccaaaat accagactga
     8461 gccaagaatg gcaaatcttt aaagagcaaa acggcagcca gaccacctac aacatagatc
     8521 ccaatatgcg ttaaaatcac tagaaacaga gccatcatcc gcgcatagaa atagtgactt
     8581 gcccttatgc tagaaaagac gacttccata attttggtgc ctttttcact ggcaacttcc
     8641 tgagctgtta cacccgcata ggtaatcaga atcatataaa gaaggaatcc taaggcacct
     8701 gctgcaattg tttgaataaa ctttttattt tccttggctt catcaatctt ttctgtgaat
     8761 tgaattgtct gcgctaagcg tttttcctgc tcttgagaca aggaagcagt tgaacgatta
     8821 agctgatttt gcagttcatt gagtgtacct gtaacctcaa atttaattcc attttcaagc
     8881 gatgtttcgc catgataaac tgcctttaga acactatctt cttgatcaat ggtcaaataa
     8941 ccttttaatt tttcttcttt aattgcttct ttggcacttg cttcgtcttt atagtcgaag
     9001 ttaacaccat ttacattctt cagtccttct gctacagatg gcactgttgt cactactgcc
     9061 actttattat ttttagccat agaagaacct tggagatgcc caattcctac agagattcct
     9121 aaaaagagga acggcgaaat caccataaag aagaaactcc atgactcgac atgtcgaaga
     9181 taggtttcct tgattacaac ccacatattt ctcatacttc cactcctgat tctagtttaa
     9241 agatttcatc gatagttggc gcttgttggt caaatgttgc gatatattga ccttgagtca
     9301 agattgggaa gagttccctt ccagcgctct catcctccaa aatcaatttc caactgcctt
     9361 gtttggtcaa gctcacctgt ttgacatgag gaagattttc caattcttcc ttgcttcgtt
     9421 cacttgaaac aaagagacgc gttttcccgt attgattgcg gacatcctga actggtccgt
     9481 gcaagaccac acggccatct cgaatcatca gaatatcgtc acaaagttcc tcaacattgg
     9541 tcatgacatg gtcagaaaag ataatggttg ctccgcgctc tttttcctga aaaatgactt
     9601 gtttgagcaa ttctgtatta actgggtcca atccactaaa aggctcatcc aagataatca
     9661 ggtctggttc atgaatcaga gtaataatga gctgaatctt ctgctgattt ccttttgaca
     9721 gactcttgat tttatctgtc agctttcctt tcacttccaa cctcttcatc cattgaggga
     9781 gtttttcttt gacttctttg gcatccatgc cttttagagt cgccaagtag cgaacttgtt
     9841 caagaactgt caatttaggc atgagactgc gttcttcagg cagataacca atccgagcat
     9901 aggtctcctg acgaatatcc tgaccatcca gaccgatttc tccctgatat tctaggaatt
     9961 tcaaaatact atggaaaatc gttgtttttc cagcaccatt ttttccgact agtcccaaaa
    10021 tacgacctgg tcgcgcttga aagtcaatac caaacaaaac ttgcttggag ccaaaacttt
    10081 tctctagact tcttacttct agcatctttc acctccgaaa tttcttgcac tcattatact
    10141 cctttttgat agcctttaca atgtttttgt ccatttttag aagactattg ctgtgtaaaa
    10201 tatagcctgg agcactttta tactcaatga aaagcaaaga gcaaactagg aagctagccg
    10261 taggctgctc aaagtacagc tttgaggttg cagataaaac tgacgaagtc agctcaaaac
    10321 actgttttga ggttgtggat agaactgacg aagtcagtaa ctatatctac agcaaggcga
    10381 agctgacgtg gtttgaagag attttcgaag agtattagtg ataaatccat tatacagtag
    10441 caaacttaat ttataccttc cgctcctcaa ctgtctattt ttaatcctga attgttattt
    10501 gagtaactcc tttttcctcg taaagttttc ttcctctaaa acttctggaa aaaggctaat
    10561 agtttcagac aacattttta taagaaacaa gttcatctgt catttcaaga aggagtaatc
    10621 ctttatctac taatggacgg aacagaattc aaccgcttgt ccgatatgtt ttctaaggat
    10681 tatatagtaa aatgaaataa gaacaggaca aattgatcag gacagtcaaa ttgatttcta
    10741 acaatgtttt agaagtagat gtatactatt ctagtttcaa tctgctatat ctattatgca
    10801 catcactata ggatctaatg aaaatcacaa caggctcatt catagatggt tacctcaggg
    10861 aactaagaaa acaactacaa aggaagtcgc atttatcgaa aagtggatta acaactatcc
    10921 taaaaaatgc ttgaactaca agtcccccag agaagacttc tggatgacta acttgaactt
    10981 gaaatttagc aataattaac tcaccatcta actatattta gtaattattt cagaactgat
    11041 taatattaaa attaactaac aattcaaagg attcatacta gccataaatt acgtccatca
    11101 gagagagact cttactactt ttagatttta gtctttctag cttcagaata catctaaact
    11161 ttagggaaaa tgactattcg aaagcgcgaa tgcctcaaaa ttatctcaga taagctattc
    11221 gaaacttaga atgcttttaa atttatggaa ttccgattat tcgaaaccta gaatgcatat
    11281 aacctttagt tgacagacct attctaagtc tcgaagggct atttactttc tattccttat
    11341 caaaaaagac tcattccccc tttctcctcc aaaatatggt atagtagaaa tatactatct
    11401 atgaggagtt tacatgtcac aggataaaca aatgaaagct gtttctcccc ttttgcagcg
    11461 agttatcaat atctcatcga ttgtcggtgg ggttgggagt ttgattttct gtatttgggc
    11521 ttatcaggct gggattttac aatccaagga aaccctctct gcctttatcc agcaggcagg
    11581 catctggggt ccacctctct ttatcttttt acagatttta cagactgtcg tccctatcat
    11641 tccaggggcc ttgacctcgg tggctggggt ctttatctac gggcacatca tcgggactat
    11701 ctacaactat atcggcatcg tgattggctg tgccattatc ttttatctag tgcgcctata
    11761 cggagctgcc tttgtccagt ctgtcgtcag caagcgcacc tacgacaagt acatcggctg
    11821 gctagataag ggcaatcgtt ttgaccgctt ctttattttt atgatgattt ggcccattag
    11881 cccagctgac tttctctgta tgctggctgc cctgaccaag atgagtttca agcgctacat
    11941 gaccatcatc attctgacca aaccctttac cctcgtggtt tatacctacg gtctgaccta
    12001 tattattgac tttttctggc aaatgctttg acacgtaaaa aatccgtttg gtttcccaag
    12061 tggattttta aagcgtagat taactatagc ttgatactaa atatactttg gtatggaaat
    12121 catgcatatt tttcgatagt gaagcgagga cttacctagc ctttccaccg tgatagaaac
    12181 acctgaaatc taatggtttc aggtgttcgg aaactttgag cctagtgtct caaagtttag
    12241 gtatggaatt tcgaagaaag tcgctaccgt ccgtaatcac ttaaggaaag gctcaaaaat
    12301 attgttttca accacaaaat ccgtttggtt tcccaagcgg attttgtgct ttattttgaa
    12361 acttcttttg caagaacaaa gttcccaagt gtggcagaac catttcctgc gactgctggc
    12421 gtcacgatat agtcacgcac atctggtact ggtaggtaac cattaagaag agatgtaaat
    12481 ttctcacgga cacggtccag catatgttgt tgagccatga cccctccacc aaagacaatc
    12541 acgtctgggc ggaaagtcac tgtcgcatta accgcagctt gagcgatata gtaggcttga
    12601 acatcccaaa cagggttgtt gagttcaata gtttccccac gtacacctgt acgagcttcc
    12661 aaacttggac cagctgcata gccttccaga catcccttat ggaaaggaca aacaccctta
    12721 aactcttttt caatatccat tgggtgtcta gcaacataat aatgacccat ttcagggtga
    12781 cccatttcag ggtgacccac accaccgata aactcaccac gttggatgac gcctgcaccg
    12841 atacctgtac cgattgtgta gtaaaccaag ttttcgatac gaccaccagc attgttacgg
    12901 gcaaccactt caccgtaagc agagctgttt acgtctgttg tgaagtacat tggcacgttt
    12961 agggcgcgac gaagggcacc aagcaagtcc acatttgccc agtttggttt tggagtcgtc
    13021 gtgataaagc cataagtttt tgagtttttg tcaatatcaa tcggcccaaa tgaaccaact
    13081 gcaagaccag caaggttatc gaattttgag aagaactcaa tggttttatc gattgtttcg
    13141 attggagttg ttgttggaaa ttgtgttttt tctacaacgt taaagttttc atcaccgaca
    13201 gcacagacaa actttgtacc gccagcttcc aagcttccat ataattttgt catgataaac
    13261 ctcttgtttt tattttcttt attatagcat acttcgaaag tctaaatgtc tctatttttt
    13321 agattttcct ctgtaaatct tactatctaa taaaaacgaa caaacatgtc atttgttcgt
    13381 tttcacatta gagaggattg attagatttt cacttcgatc acagcatccc ccttcgcaac
    13441 tgaacctgtt gcgactggag ctactgaagc gtagtcagct gtatttgtaa cgataaccat
    13501 tgttgtatca tcaagtccag ctgtagcgat tttatttgag tcaaatgttc caagaacatc
    13561 gccaactttc accttatcac cttgagcaac ttttgcttca aaaccgtcac cgttcataga
    13621 tacagtgtca ataccaacat gaatcaaaac ttcagcacca tttcttgttt tcaaaccaaa
    13681 agcgtgccct gttggaaagg caattgaaac ttcagcatca gctggtgcat agaccacgcc
    13741 ttggcttggt ttcacagcga taccttgtcc catagctcca cttgagaaga ctgggtcatt
    13801 gacatcagca agagcgacaa catcaccgac gataggagtt acaagtgttt cattttgaag
    13861 agctgctggc gcaacttctt ctttttcttc agccacttca gcttgttttg cagctgcagt
    13921 tgcgtctact tcatcttcgt aaccaaacat gtaagtaaga gcaaaaccaa gggcaaatga
    13981 tacagctacc ataagaaggt attgtggaag ttgtccgtta ccaacataaa gcattgtacc
    14041 agggatgatg gtgataccat taccagtacc agcaagtcca aggatagaag ccaatccacc
    14101 accgattgca ccagcaatca atgaaaggaa gaatggttta cggaagcgca agttcacccc
    14161 gaagatagca ggctctgtaa tacctaggaa ggcagaaaga gcagccggga aagcaagtgt
    14221 tttcagtttt ggattttttg ttttaacacc aaccgcaaca gtagcagcac cttgagctgt
    14281 catagcagct gtgatgatag cgttgaatgg gttagcatgg tcagcagcaa gtaattgcac
    14341 ttcaagcaag ttgaagatgt ggtgcacacc tgacacgacg atcaattggt gaaccccacc
    14401 aatcaagaaa ccaccaagac caaatggcat gctaagaatc gcttttgtag caataaggat
    14461 gtagttttca acaacgtgga aaactggtcc aatgacaaag agtccaagga tagacatgac
    14521 caaaagtgtc acgaatggtg ttaccaagag gtcaatgaca tctggaacaa ccttgcggac
    14581 agctttttca aatttagctc cgacaacccc gatgatgaag gctggaagaa cggaaccttg
    14641 caaaccaaca acagggatga aaccaaagaa gttcatcgct gttacttcac caccttgagc
    14701 aactgcccaa gcgtttggaa gtgagccaga gacaagcatc ataccaagaa cgataccaac
    14761 ggcaggattt ccaccaaata cacggaaggt tgaccacaca accaaacctg gcaagatgat
    14821 gaaggctgta tctgtcaaga tttgtgtgta agttgcaaag tcacctggaa gtggcatttc
    14881 aagagcgttg aaaagaccac gcacacccat gaagagacct gtcgctacga taactgggat
    14941 gattggaacg aaaacatcac caaaagtacg gatagcacgt tggaaccagt tcccttgttt
    15001 agcaacttct gctttcatgt catccttaga tgatgttggt aatccaagta caacaacttc
    15061 atcgtacatt ttgttaactg tacctgtacc aaagataatt tggtattgcc ctgagttaaa
    15121 gaaagcacct tgaacttttt ccaagttctc aatcacttct ttattgattt tctcttcatc
    15181 tttgaccatg acacgtagac gtgtcgcaca gtgggcaaca ctattgacat tttcacgtcc
    15241 gcccaaggca tcgatgactt tttttgcaat ttcctgattg ttcatttgca aaaatctcct
    15301 tatataacat tttgttcttg tttgaaagcg attttattcg cccttacgac tattatttta
    15361 tcatgtttta aaaatatgtc aagcgttttg cagaaaaaat attctatcta cttagtgggc
    15421 aagctttaga ttgattgttt ttgactgttt catcaggaat ttcttgaaaa ataagtttga
    15481 aaccttggtt aaaaagtctt ttatttcatt atctttcaca ttttcgtaaa aatttgattg
    15541 atttcctgta tttattttat gataatcgtt tgacagtttt ttctcttttt taatataaaa
    15601 ggcttgcatt ttttatcgaa aacagttact attaaaagtg ataagatttt tggaggaatg
    15661 aatggaatgg acaactgagc gtcgttacag actttatcaa gattggacgc aagaagaaat
    15721 tcaacatata aaggaaaata tggcacaatc tccatggcat actcattacc atgttgagcc
    15781 aaaaacagga cttctcaacg acccaaatgg cttttcttac tttgatggca agtggatcct
    15841 cttttaccag aattttcctt ttggtgcagc ccacggtttg aaatcttggg tacagctaga
    15901 aagtgatgat ttggttcact ttaaagaaac tggaatcaaa gttttaccag atactccatt
    15961 agatagccac ggtgcctact ctggttctgc catgcaattt ggcgataact tattcctatt
    16021 ttatacagga aatgttcgcg atgaaaactg gatccgtcac ccataccaga tcggtgcttt
    16081 gatggacaag gagggtaaga ttacaaagat tgacaagatc ttgattgacc agccagcaga
    16141 ctctactgac cacttccgcg atccacaaat ttttaacttt aaaggtcaat actatgctgt
    16201 cgtcggtgga caggacttgg agaaaaaagg cttcgtccgt ctctacaagg ctatcaataa
    16261 cgactacaca aactggcaag cagttggcga ccttgacttt gctaacgacc gtactgccta
    16321 catgatggaa tgtcctaatc tggtctttgt agaggaacag cctgtccttc tctactgtcc
    16381 acaaggattg gataagaaag ttctagacta cgataatatc tttccaaata tgtataagat
    16441 tggggcttcc tttgacccta aaaatgccaa aatggtagat gtgtctcaac ttcaaaacat
    16501 ggattacggt ttcgaagcct atgcaactca agccttcaac gctcctgatg ggcgtgttct
    16561 agcagtgagc tggcttggtt tgccagatgt ttcttaccca tctgaccgtt ttgaccacca
    16621 aggaaccttc tctttggtca aggaactcac tatcaaagac gacaagctct accagtatcc
    16681 agtcgctgct attaaggacc ttcgtgcttc tgaagaagcc ttctcaaacc gttcccaaac
    16741 caagaacact tacgaacttg aactcaactt ggaagctaat agccagagcg agattgtctt
    16801 acttgctgat aaagaaggta agggactttc aatcaacttt gaccttgtaa acagtcaagt
    16861 gacagtggat cgtagccagg ctggagaaca gtatgcccaa gaatttggga caactcgttc
    16921 ttgccctatc gagaatcagg ctactactgc tacaatcttc atcgataact ctgtctttga
    16981 aattttcatc aataaaggag aaaaagtatt ttctggtcgt gtcttcccac atgcggacca
    17041 aaatggtatc ctgatcaaat ctggaaaccc aactggaact tactatgaat tagattatgg
    17101 tcgcaaaact aactgatgtc gccaaacttg caggcgtcag tcctactacc gtttctcggg
    17161 ttatcaataa aaaagggtat ctatctgaga aaaccatcca aaaagtcaat gaagccatgc
    17221 gagaattggg ctataaaccc aacaacctgg ctcgtagtct gcaaggaaaa tcagctaagt
    17281 taatcggctt gattttcccc aatatttcca atgttttcta tgcagaattg attgataaat
    17341 tggaacacca actcttcaaa aatggttaca agaccatcat ctgcaacagt gaacatgatt
    17401 ctgagaagga acgcgaatac atcgaaatgt tggaagccaa tcaggtggac ggcatcattt
    17461 ctggtagtca caacctagga atcgaagact acaatcgtgt gacagcgccg attatttcct
    17521 ttgaccgaaa cctatcgcca gacatccctg tcgtctcctc tgacaactat gctggtgggg
    17581 ttcttgctgc ccaaaccttg gtcaagacag gtgcccagtc tatcatcatg attacaggga
    17641 atgacaattc caattcgcca accggactgc gccacgctgg ttttgcatcc gtactcccaa
    17701 aagctcctat tatcaatgtt tccagtgact tttctcccgt cagaaaagag atggaaatca
    17761 agaatatctt gacccaggaa aaaccagatg ccatttttgc ttcggatgat ttgacagcta
    17821 ttctggtcat taaaatcgct caagaattgg gcatttctgt cccaaaagag ctcaaggtca
    17881 tcggctatga tgggacctac tttatcgaaa attactaccc tcaattggct actatcaagc
    17941 aacctttgga agagattgct tgtctcacta ttgatcttct tttgcaaaag attgaaggca
    18001 aggaagtcgc cacaactggt tacttcttac cagttacact attaccagga aaaagtattt
    18061 aaacacaaga aaactcagac cgattcgtct gagtttttta tgatcttaaa ttttcgagat
    18121 agcgctgggc tgtctctagg ttaaaggttt tatctgagat gaggcgctct actaggggag
    18181 caacttcaga ttcactagcc ccagctagga gagctaggga tttggcctgt agtttcatat
    18241 ggccttgctg gatgcccgta cttaccaagg ctttgagggc tgcaaaattt tgggcaagac
    18301 cgatggacac gataatctgg gctaattctc tggcagaagg atttcctagt agatcatgac
    18361 tgagagctac acgtgggttg aggccgatag agccaccctt agtcgctaca ggcatgggca
    18421 gggtcatctc accgaccaat tcttctcttt caaggtccag cgtccagcag ctaagacctt
    18481 gatagcgtcc atctcgactg gcaaaggcat gggccccagc ttcgatggca cgccagtcat
    18541 taccagtggc aatcaaaatc gcatcaatac cattaaaaat tcctttatta tgagtagcag
    18601 ctcggtaagg atcagcctgc gcaaactgac tagccaacgc aattttctcc gcaatctctc
    18661 gtccttgatc cttttggcgg ctcaagtagc gaaaggcgat gcgacagctt gcagtcacca
    18721 gagaatcggt cgcgtagttg gacaggattg ccatgagact ctgtccctga ctgagttctt
    18781 ctaagactgg tttcaaggct tccagcatgg tgttgagcat attggcaccc atggcttcct
    18841 gggtatcgac atgaatataa acaacgagaa agtctggttc accttttatc tgctcgacat
    18901 gcagatcacg cgccccacct ccacgtttaa cgatagaagg ataggcttga ttggcaagct
    18961 ccaagagctc cgctttcttg ctggcaatct tctcttgcgc tagtttagga ttagcaattt
    19021 gataaagggc tacctgccca atcatctggc gctgatggac ttgtgcagta aaaccacctg
    19081 cacgcttgat gattttgctg gcatagctgg ccgccgcaac cacagagggt tcttctgtca
    19141 catagggaac ggtgtattcc tgaccgttga caagtacctc cggaaccagc gaataaggca
    19201 gagaaaaagt tcccaccaca ttctcactca gctggtctgc cacagtcaca ctcatctgtt
    19261 catccttctc cagactagct tgtctctcag gactaaggag cgcctgagct tttagcagct
    19321 cgaggcgctc ttggtatgat tttttagaaa atccattcca acttatcttc attatttttc
    19381 aaccttgcta taacggcgtt ggtggtcaag aatttcaacc aaggcaaaat cttgattttc
    19441 atagccagca aactgggcag agtttgtttc atccaagttt acttcctcaa aaaagacctt
    19501 ttcatagtct gcaacggata gggcagttcg ttggttgagc ttgttcaagc ggtctttatc
    19561 caaataagct tcatatcctt caaccaattc accactgaag aactcagcca cagctccact
    19621 tccgtaacta taaagggcga ttttatcccc agctttcaag ctatctgtat tttccaagag
    19681 agacaaaagt ccaaggaaaa gtgaacctgt gtagatattc cccacctttt gactgtagag
    19741 aatagactgg tcaaaatgct tttgtaagag gtcttttttc tcttgaggca ggctcttatc
    19801 cataattttt ttcaagcctt ttagcgctaa tttaggataa ggcaagtgga aacaaacagc
    19861 cgcaaaatca tccagagtaa gctggtagcg tttttgatat tcaagccaag tcgttttcaa
    19921 actatccaag tattgttggg tagaataaac accatttaca taaggagttg tcgagtaatt
    19981 tggtcgccag aaatccatga tgtcacgggt ctgagctaca ttgtcattat taaaggccat
    20041 catgcgtgga ttttgtgtaa tcaacatagc tacacttcca gcaccttgag ttggttctcc
    20101 tggagtttca ataccgtatt tggcaatatc actggcaatg accaagacct tggactccgg
    20161 agaattttcc acatgcaatt tggcataatg aagggcagca gtcgctccat aacaggcttc
    20221 tttaatctcg aaactacgag caaagggctg gatgcccagc aagccatgca caaagacggc
    20281 cgcagcctta ctctggtcaa ttcctgactc ggtagccaca atgaccatgt caacttcttg
    20341 tctttcttgc tcagttaaaa tagagtcact agcactggcc gccaaggtca caatgtcctc
    20401 agttaggggc gcaatactca attccttgag taagagtcct ttacttaatt tttcagggtc
    20461 aattcccctc gcttctgcta agtcttgtaa tttcaagaca tattgactgg tcgcaaaacc
    20521 aatcttatca ataccgattg tcatatttac ctctgtttta tcattcatgt aaaaaatcgt
    20581 tctatactat tttatcacaa atggcagtaa aagagagaaa aaagacttga ttcaccaaat
    20641 caagcctctt attggtcatc attttaaaga atgattagtt gctagagagt tcaccgatat
    20701 aagtagcttt ataagctcca ttcacagtta tcagctcctg gaggatcaaa tttcctgagt
    20761 aagtccttcc catctcatct acaaattttt gataaaactg actggtcgga atttctctga
    20821 catccttatc aaatgtctta tcaagtgttt tactaacctt ctcagcaatc aattgatgct
    20881 cttgccatcc actttgaaac tctgagctcg aactagaaac catgactggg ataaacaaca
    20941 aggtcagtag atttacagac aataaggaaa gtagtagact tcctgcaaaa ctagaatcct
    21001 agttcatgat tgataatacc agcaatcaaa ttcattcgta atcc
//
LOCUS       DAVKHZ010000034        20117 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000034 DAVKHZ010000000
VERSION     DAVKHZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.034"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..363)
                     /locus_tag="VWW99_001134"
     CDS             complement(<1..363)
                     /locus_tag="VWW99_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538984.1"
                     /translation="MEIYTYSDFLKDKTALTFEEAGEIYNNLVSSLNTKDPDFLENWT
                     TLIELAAQYTELRVRSETIPLPKREQNDISRKSTHKAFISQLKVIKYFSENDGKNTNW
                     FDQITNDRQRQGDFANYLA"
     gene            1109..1261
                     /locus_tag="VWW99_001135"
                     /pseudo
     CDS             1109..1261
                     /locus_tag="VWW99_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1417..1755
                     /locus_tag="VWW99_001136"
     CDS             1417..1755
                     /locus_tag="VWW99_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU9538985.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            1945..3429
                     /locus_tag="VWW99_001137"
     CDS             1945..3429
                     /locus_tag="VWW99_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9538986.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFAAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVEYAKQTEIILSLFSILG"
     gene            3433..3672
                     /locus_tag="VWW99_001138"
     CDS             3433..3672
                     /locus_tag="VWW99_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU9538987.1"
                     /translation="MIGSIAAILTTFAFLPQVFRIVKTKDTGSIALGMYVMQVIDIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            3802..4116
                     /locus_tag="VWW99_001139"
     CDS             3802..4116
                     /locus_tag="VWW99_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU9538988.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            4234..6213
                     /locus_tag="VWW99_001140"
     CDS             4234..6213
                     /locus_tag="VWW99_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9538989.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDATERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            6223..6537
                     /locus_tag="VWW99_001141"
     CDS             6223..6537
                     /locus_tag="VWW99_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU9538990.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            6568..7089
                     /locus_tag="VWW99_001142"
     CDS             6568..7089
                     /locus_tag="VWW99_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538991.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKFINEQIRLINNLSN
                     WKRTHLVYNPDLV"
     gene            7153..8499
                     /gene="celB"
                     /locus_tag="VWW99_001143"
     CDS             7153..8499
                     /gene="celB"
                     /locus_tag="VWW99_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU9538992.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVF
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            8879..9055
                     /locus_tag="VWW99_001144"
     CDS             8879..9055
                     /locus_tag="VWW99_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9538993.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            9456..11669
                     /locus_tag="VWW99_001145"
     CDS             9456..11669
                     /locus_tag="VWW99_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9538994.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(11891..12367)
                     /locus_tag="VWW99_001146"
     CDS             complement(11891..12367)
                     /locus_tag="VWW99_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9538995.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            12590..15793
                     /locus_tag="VWW99_001147"
     CDS             12590..15793
                     /locus_tag="VWW99_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9538996.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKHGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            16146..16531
                     /gene="tnpA"
                     /locus_tag="VWW99_001148"
                     /pseudo
     CDS             16146..16531
                     /gene="tnpA"
                     /locus_tag="VWW99_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(16910..17539)
                     /locus_tag="VWW99_001149"
     CDS             complement(16910..17539)
                     /locus_tag="VWW99_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9538997.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(17549..18550)
                     /locus_tag="VWW99_001150"
     CDS             complement(17549..18550)
                     /locus_tag="VWW99_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9538998.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRTILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(18581..19222)
                     /locus_tag="VWW99_001151"
     CDS             complement(18581..19222)
                     /locus_tag="VWW99_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9538999.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFDEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(19241..20056)
                     /locus_tag="VWW99_001152"
     CDS             complement(19241..20056)
                     /locus_tag="VWW99_001152"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9539000.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         6507 a         3140 c         4107 g         6363 t
ORIGIN      
        1 tgctaaataa tttgcaaaat ctccctgacg ttgtctatca tttgtgattt gatcaaacca
       61 attagtattt tttccgtcat tttcagagaa atatttaata acttttaatt gacttataaa
      121 agctttatga gtagattttc tggaaatatc attttgctct ctctttggca gtggaattgt
      181 ttcagacctg actcgaagtt ctgtatattg cgctgctaat tcgattaacg ttgtccagtt
      241 ttcaagaaaa tcaggatctt ttgtattcaa agagctcact agattgttat atatttcccc
      301 agcttcttca aaagttaatg cagttttatc tttcagaaag tctgaataag tgtaaatttc
      361 cataaagggc ctccacaaaa aaaatatttt ttgtctaaat acgactatac ttttattata
      421 attctatcta aaaataatgt caaggatttt cgtaaaaaaa ttttaattag ttttacaaaa
      481 gtattgcgca ataggatgtt aagttttgat atttgtagta taattagtac tgactgattt
      541 catcaattag ggcaaatttc tgagatatct gactcgtatt tttttattgg ataaaaaatt
      601 tgccctattt gaatgaaatt atttaaaaat agataaaatt taattgataa ttgattgtaa
      661 aaaagtccgt gtttataact atttgtgata ttttaattta atagttagcc aaaccaggtc
      721 ttaagaaagc tcgtaaagca tcacaattta gtaaacgtta attcgaaaga attactatac
      781 ttatacagag cacctttcgg ggtgttcttt tttatacttt cttactaaat tggtgcaatt
      841 gacacagttg ttgcgacttt agtcgcttac aaatgtggct gcaacctgac aaggtcagtt
      901 gcctcaaaac gttaatcaat acgattatat caacgtttca aagcactcaa aagtttacct
      961 tatgggtgct tttttcgtgt ttttttgaaa agtttacctt atttttatag taatgtgaaa
     1021 taagaatagg acaaactgag gatagtcaaa tcgaattcta acaatgtttt agaagtagag
     1081 gtgtactatt ctagtttcaa tctactatat tatggataat gcaagattcc atagaatggg
     1141 taagttagaa ctcttgtgtg aagagtttgg gcataaactt ttacctcttc ctccctactc
     1201 acctgagtac aatcctattg agaaaacatg ggctcttttg tcctgctctt gtttcaattg
     1261 actataaatc gctataagaa aaaatcattt tattggtata aagaagtgat tgaaagtaat
     1321 ggcgaaacgt tagataatta gttcataaaa aacaagttta gatcctcatc taaacttgtt
     1381 tttttaaaat tgaaggatga ttttgaagga tataagatga cattgactat tcatgtatat
     1441 tataaaggtg atggagatag tgcaataaag tttgcaagag aaatgcttga tagtggcctt
     1501 gttgaagaaa ttagaaatca aaaaggtaac ttgaaatacg aatatttttt accaattgaa
     1561 aaagagggaa ctattctcct tattgatcag tggataaatc aagaagcttt agatgaacat
     1621 catcaatcaa agacaatgca gaaaatctta gatttaagaa aaaaatatca cttgcagatg
     1681 caagttgaac gttatataga agatgattct gggatgccag aaagcgataa gtcgtttata
     1741 gacacaggga attagaatta ttgtgagagt ttttgtagtt tgaaagtatt gaaaatcgta
     1801 aagatatcat tacgagaata caagaagata ttgttctatt tccattaaaa attacttctt
     1861 tccttaacaa taaggaaaaa gaatgcgttt tcatcttttg gtttgaggtt tataataaga
     1921 gtatcaaagt caggaggaaa aaagatgact acttttaaag atggattttt atggggtggt
     1981 gctgttgctg ctcatcaact tgaaggtgga tggcaagaag gtggcaaggg aattagtgtt
     2041 gctgatgtta tgactgctgg tcgtcatgga gtagctcgtg aaattacttc gggagtttta
     2101 gagggtaaat attatccaaa tcatgaggcg atagattttt atcatcgtta taaagaagac
     2161 atagcacttt ttgctgaaat gggattcaag tgcttccgta cctctattgc atggacgcgt
     2221 atctttccaa aaggtgatga gttagagccg aatgaagaag gattacagtt ttatgataat
     2281 ctttttgatg aatgcttaaa gaatggtatt gaacctgtca tcactctatc tcattttgaa
     2341 atgccttatc acttagtgac cgaatatggt ggttggaaaa ataggaaatt gattgatttc
     2401 tttgctcgtt ttgcagaagt cgtatttaaa cgttacaaag ataaggttaa atattggatg
     2461 actttcaatg aaatcaataa tcaagcgaat tatcaggaag attttgcacc atttactaac
     2521 tcaggtattg tatatgagga aggtgataat agagaagcaa ttatgtatca agcagcacat
     2581 tacgaattag ttgcttctgc acgagctgta aaaattggtc atgagattaa tccagatttt
     2641 caaataggtt gtatgattgc gatgtgtcca atttatccag ctacttgcaa tcctaaggat
     2701 atcttaatgg caatgaaagc tatgcagaag cgttattatt ttgctgatgt gcatgtttta
     2761 ggtaaatatc ctgagcatat tttcaagtat tgggaacgaa aaggtatttc agttgatttt
     2821 gctgcccagg ataaagaaga tttacttggt gggactgtag attacattgg tttcagttac
     2881 tatatgtcct ttgctatcga ctctcatcgt gaaaataatc cttattttga ttatcttgaa
     2941 acagaagatt tagtgaaaaa taattatgtt aaggcttctg aatgggagtg gcaaattgat
     3001 ccagaaggtt tacgttatgc gttaaattgg tttacagacc actatcactt accactcttt
     3061 attgttgaaa atggttttgg agctatagat caagttgcag cagatggtat ggtacatgat
     3121 gattatagaa ttgaatatct aggtgcccat attcgtgaaa tgaaaaaggc tgtagttgaa
     3181 gatggtgttg atttaatggg ttatactcca tggggatgta ttgatttggt ttcagctggt
     3241 accggtgaaa tgcggaaacg ttatggtttt atttatgtag ataaagatga caatgggaag
     3301 ggaagttata atcgttcccc gaaaaaatct tttggctggt ataaggaagt tatttcatct
     3361 aacggtgaat cagtagaata tgctaaacaa actgagatta ttctcagttt gtttagcata
     3421 ttgggatgac atatgattgg aagtattgca gcaattttaa caacatttgc atttttacca
     3481 caagtttttc gtatagttaa gacaaaggac acaggatcta ttgctctagg tatgtatgtt
     3541 atgcaagtta ttgatattgc attgtggctt gctcatggta ttcgtatagg agacttgcct
     3601 ttgattttag caaatagtgt ttcatttcta ctctcaggaa taattttatt ttataaattg
     3661 aagtataaat aatttttttc catatcattt aggaaaatga gggaattgtc attggaaacg
     3721 ctttatttta taataaaaat agataacaga taagtcaact tagcacagct ttgattggat
     3781 ttaagaaaag gagaaaatat tatggcaaaa gctttaatta tttgtgcagc aggaatgtct
     3841 tcatcactta tggcacagaa aacaacagag tttctaaaag gaaaagggaa agatattgta
     3901 gttgatgcta ttagtgctac tgaaggaggg aaagctatcg cagcagcagc ttttgatttg
     3961 tatcttgtca gccctcaaac aaaaatgtac tttaagcaat ttgaagaggc tggttctaaa
     4021 gcaggaaaac cagttgtaca aatacctcct caagcctata ttcctattcc aatgggaatt
     4081 gaaaaaatgg ctaatttgat tcttgagaat atttgatgaa cacatgaaat gtcaataaat
     4141 atttaagtta agataaattg ttttgagttt gatataaatt gatttaagtg tataatatgc
     4201 ttcaatatat gaaaacatac gttttaggag ggaatgaatg tgttaaacat taaagagaaa
     4261 gatatccttc aatttttaat aaaaaataag gaacgttttg ttacaagcaa agaactggca
     4321 gaatatttat cctgttcaga tagaactgtc cgtaatgtac tgaaattaat tgagaagaca
     4381 atgattattc aaggagtccg gttgatttca aaacaaggtc aagggtatca aatatttttt
     4441 gagaatcaag gcgcttacca ggagtttaga caaacttatg aactggaaga agactatacg
     4501 aaaactgcgg tttcaaaagg tgatgatcgt ttagtattta tcttaaataa attattattt
     4561 gagcaagttc cagttctttt tgatgattta gctgatgaac tttacgttag ccgttcaaca
     4621 ttatctcatg atttcagaaa gatacgagtg atgttatcag agtataattt gtccattgag
     4681 agtagggcga ataaaggagt ttatgtctca ggcgaggaac gtgataaacg acgttttatc
     4741 ataaattatt tcctagagaa tcaatttttt aaaacaatac attgctatgt aaaatttaac
     4801 ttttttgatc aaactgttcc attagaagaa tttgctagaa ttgttttgga tgaatgccaa
     4861 gaggccaacc taaaactatc tgattttgta ctacagaact tagtagttca catagcattg
     4921 tctgtgattc gtttaaaatc cggatttgaa ataaaaaata tagactgtca gatgactgat
     4981 gatgcaacag agagaaaggt tgcccaaaga attctttcta aagtaagaga agtaacaaat
     5041 caggaatttc cagttcagga aatagactac attactttgc atttgttagc taagagtcaa
     5101 caatgtcaaa aaaatcagaa gaatatttct gaagaagttt taaaaaaatc tttatttaaa
     5161 acatttcaga atttaggatt agatgatatg tataactttt cttcagattt ccaactaatt
     5221 gaaggattga taactcatct aatgacacta caagtacgtt tagaaagccg gattacctta
     5281 aataaccctt tagtagatga aattaaacag aattatagtg atatattctt tatgactaga
     5341 gaaattcttg caaatatgga caagttcttg gagtggacaa tatcagatga tgaagtagca
     5401 tatgtaagtc ttcacttttt agctgctatg gaacgtagca aagagagtac taagtttaat
     5461 attcttgcta tttgtgcaac aggatttggt gcagcccaaa tgttaagaaa tcgtttggaa
     5521 acggagtttg ggaagcgagt agaagttgtt gatgtaatag gctattatga attgaatcaa
     5581 gaaaaactta aaggaataga ttttatagtg tcagctgttg atttatcaaa tctatatttt
     5641 caaatcccag tttttaaagt tagtgtgttt cttaagagtg atgagatgga aatgattaga
     5701 aaagctatgg atcagatgca agtttctagt tatgtacaat catcaaagat taataaattt
     5761 gaaaacaaca gctttagaca gtattttagt aaagaaaatt ttttaatatg tactgaatca
     5821 gacaaggtaa atttacttga aaagatggtt gagagtttat ctgttggtga atcaaatgag
     5881 tttgaacaat ctttacttta tggaattaaa cagcgcgaag aattaagttc agttgttttt
     5941 tctgaaaaaa ttgcagttcc gcatcctatt cagccctttg gtacagaggg aaaggtttcg
     6001 gttgcaattt gtaaagattc gttactctgg gataatcaaa gttcacatgt ccagctagta
     6061 tttcttctat ctccatcaat atatgggaac gaaggtttgg caactgtgac taaaaagata
     6121 gtctctctga cagagaatga tgaattgcaa aatcagctaa tatcttgcaa taactttgaa
     6181 gattttatta acatatttga gaagataaaa taggaggttc atatgaatca agaggaactg
     6241 caggttgcag catttgaaat tatcctgcat tcgggaaatg caaggtcaga aatacatgaa
     6301 gcatttgcaa aaatgagaga aggtagtttt gatgatgcag aaagtaaatt aaatcaatca
     6361 aatgagataa ttcttgaagc gcatcatgca caaaccaaac tcttgcaaga atacgcaagt
     6421 ggagtggaaa ttaaaattga aattatcatg gtacatgctc aagatcattt aatgaccact
     6481 atgacattat tagaagtagc aaaggaaatg ttagctcttt ataagaaagt taactaaata
     6541 tgtgttgtta tataaggagg aaatgatatg gataaaaaag aacgacaaaa gattgaacaa
     6601 caacgtcgag agatggctct aactaatact ttttttaatc gatatcttct tctacgttat
     6661 tcaatagcac tcttcttttt tgggaatatt tattggcttt tgaatcagtt tattagtccc
     6721 tcgcctatta ttattttccc cattatgctc atagtatttt ctattttagc aactattgaa
     6781 caatttaaac tttatggaaa taggaaagaa aagttgggaa taacactaat gtttgttaga
     6841 atacaaatac ttatttcaat aggattactt gttttaactt ggacaagttg gtttaagaat
     6901 ctttttccga tttttgaaaa taaccaagta gcacgtttat ttgtttttgt agttcttcta
     6961 ttaggtttag ttctgagttt gttagacatt agaagaatta aaaaatttat aaacgaacag
     7021 ataaggctta tcaacaattt gtccaattgg aaaagaactc acttagttta taatcctgat
     7081 ctagtataat ttgtatttga aactagtctt tagaaagtaa aaattattat gttttattaa
     7141 ggaggtctat ttatggatca acaaaacggg ttgtttggtt ttcttgaaaa ccatgttatg
     7201 ggaccaatgg gcaaacttgc tcagtttaaa gtagtgcgtg ctatcacggc tgcaggtatg
     7261 gctgctgtac catttactat tgtaggatca atgtttttgg tattcagtat tttgccacaa
     7321 gctttctcat tttggccaat tgtggcagat attttctctg cttcatttga taaattcaca
     7381 tcactttaca tggttgcaaa ctatgcgact atgggttctc tatctcttta tttcgttcta
     7441 tcacttgcat atgaattgac aaaaatttat gcagaggaag aagaactcaa tatgaatcct
     7501 cttaatggtg ccttgcttgc cttgatggct tttgtcatga cagtaccgca aatcattttt
     7561 gatggtggaa tgatgaagac tgtgacaagt ctaaaagaag gtgcagtaat tgcagatgga
     7621 tgggcaatgg gaaatggagt cgcacgtttt gggacaacag ggatttttac cgcaatcatt
     7681 atggcaattg tgactgttct tatttatcgt atgtgtgtta aacataattg ggttattaaa
     7741 atgcctgaag ctgttccaga aggagtttct cgtggattta ccgctttggt tccgggattt
     7801 gtttttgcat ttgttgttat ctttatcaac ggtcttcttg tagcaatggg aacagatatt
     7861 tttaaagtca ttgcaattcc atttggtttt gtatccaatc tgactaattc gtggattggt
     7921 ttaatgatta tttatctatt gactcaacta ctttggattg taggtatcca cggtgcgaac
     7981 attgtttttg catttgttag tccaattgct cttgctaaca tggctgaaaa tgctgctggc
     8041 gggcacttcg ctgttgcagg tgaattttct aatatgtttg taattgcagg tggttctggt
     8101 gcaactttag gactatgttt atatattgct tttgcctcta aatctgaaca gcttaaagca
     8161 ataggacgag catctgtagt tccggcctta tttaatatta atgaaccatt aatttttgga
     8221 ttacctatta tctataatcc agccttggct ataccattta ttttagcacc aatggttact
     8281 gctactattt attacgtagc gaattctcta aactttatta agccaattat cgcacaggtt
     8341 ccatggccaa ctccagtagg gattggagct ttcttaggga cagcagatct tcgagctgta
     8401 ttagttgctc tagtatgtgc atttgcagca ttcctagtct atcttccatt catccgtgta
     8461 tatgatcaaa aattggtgaa agaagagcaa ggtatctaat aagtaaaaaa gtatactcga
     8521 tgatagaatc catatagttt aaataatgag tgttaatatt agcagtgaag aggtcttttt
     8581 aagatattct tctcttcttt ttcatatttt ttcgctatgt agggtatgaa gcaaaataga
     8641 aagtagttcg aatagttgat aaacaggaaa ctgctgttat ttaaaaaaaa caataaagta
     8701 aagtatgtta tcgtagaata cgatacaata gtaaaaaagc aaaaagaatg cgttttatat
     8761 ttgggtttga ggtttataaa ataggataag cgtgctgaaa acttagatat gacaaacaaa
     8821 ataaaatttt taaaagctac atagaaacat ggtataatgt atctaaagga ggtagcatat
     8881 gtccttagat atagataaag aaaagatgac aatcatggga atagcttttg aaaacagatc
     8941 cgtttttaag agtgtttggt atgctttaag tacaaatatg attgaaggat ggcgacctac
     9001 ggttagtgat gttgaaaaat tacgagatga agctcttgct ctggggatga cttaatgaag
     9061 cgcaactgtt atgagtctta tgactatgtt gatccggata atttatatac cgatccgggt
     9121 tcttcagttc taagaaataa gcaggaagga aaaaagcacg cgagcttgaa tatcgtatga
     9181 ttgctagtaa gagtttgaaa ttatttatca atcctatctt agttaatagt ttaaaagtag
     9241 tttgtttcac aactgcataa gcttagaaaa gttgaaaagt gataactctt taataaactc
     9301 ttggtttgtt aaaagatatt ttgtccacta cattttttca attgactatt atttgtcata
     9361 gatataacag atagaacatt gcattcagaa taatgaaaaa cgcatcaaaa atggtgcgtt
     9421 ctgtttttat gctaccataa aaggaggaaa acaaattgaa aatttttaag ggagagtttt
     9481 atcgaatctc tgtattaaca gacaagctag taaggttaga atactctcaa actggaagtt
     9541 ttgaggatag aacgacacaa cttatctata atagagattt tggccaagtt tcgttagatt
     9601 atatcgagac atcaaacgta ctagatatta tgacggacta ttttcatctg cactttaata
     9661 aaggagaatt taacgccgaa aatttattta tagaattaaa aggaaatttt gccgtatatg
     9721 gtagtcgctg gtattttggt gaatctattg aaacgttaaa aggaacagct cggactctgg
     9781 ataaggcaga tggagcaatc tcgttagaag atggaattat tagccgaaat ggtatagcct
     9841 tattggatga ttctcaagga tttatttggg atgaacaatc tggttatatt gagagagaaa
     9901 atcaaattga cctgtatttc tttgcctatg ggcatgatta tagaggagca atcagagact
     9961 tttaccattt gactggttca acacccttgt tgccaagata tgctttaggc aattggtgga
    10021 gtagatattg gccttatacg tcggatgaat atttggattt aatagacaga tttgaaacag
    10081 agaaaattcc attatctatc ggtgtgttag atatggattg gcatataact gacattccag
    10141 ctcgttttgg aagtggctgg acaggatata gttggaatag aaacttaata ccaaatccag
    10201 aacagttatt gcaacaactt catgatagaa agttaaaact ctccttaaat gtccatcctg
    10261 ctgatgggat acgggcttat gaagaagctt atcctcaaat cgcaaaacgg ttggggttaa
    10321 atgtagaact agaagaacct gctatttttg atttttttaa tccctctttt agggaagcct
    10381 actttaaaga tgttcattat gagctagaaa agcagggagt agatttttgg tggattgact
    10441 ggcaacaagg gacacaaggt atgctagatc cactttggct tttaaaccat tatcactatc
    10501 aggatagttg taaaaatgca gaaggtggtt tgattttatc aagatatgca ggtcctggta
    10561 gtcaccgcta ccctgttggt ttttcagggg atactattat tagttggaat tccttaagat
    10621 ttcaacccta ttttacagcg acagcatcta atatcggtta tagttggtgg agtcatgata
    10681 tcggtggaca tatgttgggg gattatgacg aagagctaca aactagatgg ctacagtttg
    10741 gtgtttttag tccgattact cgattacata gttctagaag tccttttaat agtaaagaac
    10801 cttggttttt ttcagaaaca acatctaaga ttatgaagaa ataccttcgt ttgagacatc
    10861 agatgattcc ttatctatat accatgaatg taaagacaca tgaggaaggt gccccattaa
    10921 tcagtccaat ctattatttc tatccagaga atgatgagag ctataatgtt ccaaaccaat
    10981 acttttttgg aacagaactg atggtggctc ccattgtaga aaagatggat ttgacattcc
    11041 aatctgcaaa agtagatgta tggttccctg aaggtgaatg gtatgacttc ttttcagaga
    11101 aaaaatacac aggtggtgtg aagttaagtg tttataggga catctcgact acgcctgtgt
    11161 ttgcaaaaag tggtgcaatc attcccttgg ttggttctga gataggtatg ggtgttgatt
    11221 tacctgaggt tgtagattgg tatgtattcc cagggaaaca acattctttt gaaatgcttg
    11281 aagatcaaaa tggtcaaaga tataaaacaa gattatcaat cgattgggaa atgggaatgg
    11341 tagagttagc attacaagga gattctagta tcgttccaag caatagaaaa catagaattc
    11401 attttaaagg aacgaatgtg tctataattg aattgccaaa taagaatgat acagctaaat
    11461 ttgaatggaa agataataaa aggacatctt taaatgacga agtttttaga ctactaaaga
    11521 cagcttctct tccatatgaa ttaaaagata gattgttaaa tcaattcatc aatgccaaaa
    11581 actctcatga cttaatgaat atcttgcatc atcaggataa ggaattgaga gggcgtttgt
    11641 tggaaatgat atttactagc caaaactaat tgaaacaatt agaaagtcat ttgtttagtg
    11701 tatatgaaga ttctgtatgg tattcgttat cactggtcac attgaaaatg tgaaataagc
    11761 acctacaaca tagactttac aacacttcat gtttcatatc atggggtgtt tttctattta
    11821 agtcaaagcc aatcaaaaaa tctcactatt catcaggtta aaggaaacct aatcatagtg
    11881 agattgtgaa ttatagtaga gtttgtatcg cctcttcaat ttgttttggg tctgtttttg
    11941 aagaaaagcg ttcaaagact tgcccatctc gaccgatgag aaacttagcg aaattccatt
    12001 cgattcgttt tcctagtggg ccagatttct ggtcttgtaa ccagacatag agagggtctg
    12061 cttccttacc gttgacctta atcttggcaa aacgtgggaa ggtggtttga aaatgtaggc
    12121 tacagaaggc gttgatttcc tctgcgctgc cgggtgcttg tcccataaac tgattgcaag
    12181 ggaaatccaa tatttcaaag ccctgatctt gatagcgttc atagagttct tgaagtccct
    12241 ggtactgggg cgttaaacca catccagtag cagtgttgac aatcaagaga actttgccac
    12301 gatagctatc caagggagtt gcttggttgt tttggttcaa aacggaaaaa tcataaagtg
    12361 aagtcatgag ggcctctttt ctttttctac attgtaacag tttttacttt tttcgtcgag
    12421 tcggatggaa gatattgttg aagaataagc cctcaatatg atagaaaccg tttacaaata
    12481 actttaaaaa tggtataatt taattaaacc ggtttactta gttgctataa atctctaatt
    12541 tgagatttag ttcctattga ttttacaata tgtttattgg agtgtataca tgcaaacaaa
    12601 aacaaagaag ctcattgtga gtttgtcttc acttgtttta tcaggatttt tattaaacca
    12661 ttatatgaca gttggagcgg aagaaacgac tacgaatacc attcagcaaa gccagaagga
    12721 agttcagtat cagcaaaggg atacaaaaaa tttagttgaa aatggtgatt ttggtcagac
    12781 ggaggacgga agcagtccgt ggacaggaag caaagctcag gggtggtcag cttgggtaga
    12841 ccagaagaat agttctgcag atgcctcaac tcgagtcatt gaggctaagg atggggctat
    12901 cactatctca agccctgaga aattaagggc agcggttcac cgtatggttc ctattgaagc
    12961 taagaaaaag tataaactgc gtttcaagat taaaacagat cataaagtcg ggattgccaa
    13021 agttcgtatc attgaggaaa gtggtaagga caagcgattg tggaattctg caacgacgtc
    13081 aggaacaaag gactggcaga ccattgaagc agactatagc ccgactttag atgttgataa
    13141 aatcaagctg gagttattct atgaaacagg aactgggact gtttccttta aggatattga
    13201 gctggtagag gtagcagacc agctttctga ggattctcaa acagataaac agcttgagga
    13261 aaagattgat ttaccaattg gaaaaaaaca tgttttttct cttgcggact atacttataa
    13321 ggtagaaaat cctgacgttg cttcagtcaa aaatggaatt ttagaacctc ttaaggaagg
    13381 gacaaccaat gtcattgtca gtaaagatgg caaggaagtg aaaaagattc ctttgaagat
    13441 tctagcctct gttaaggatg catacacaga ccgtttggat gactggaatg gcatcattgc
    13501 tgggaatcaa tactatgatt ctaaaaatga acagatggcc aaattaaacc aggaattgga
    13561 aggaaaggta gctgatagcc tatctagtat ttcaagtcag gcggaccgca cctatttgtg
    13621 ggaaaaattt tcaaattata aaatgtctgc aaatctgact gccacttatc ggaaattgga
    13681 ggagatggcc aagcaagtga ccaatccttc ttctcgttat tatcaagatg aaactgtcgt
    13741 tcgaacagtc agggattcca tggaatggat gcataaacat gtctacaata gtgaaaagag
    13801 cattgttggg aactggtggg attatgaaat cggtacacct cgtgccatca acaatacctt
    13861 gtctctgatg aaagaatact tctctgatga ggaaattaaa aaatatacag atgtgattga
    13921 aaaatttgta ccagatcccg aacatttccg aaagacgact gataacccat tcaaggctct
    13981 aggtggaaac ttagttgata tgggaagggt aaaagtaata gctggtttac tgcgtaagga
    14041 tgatcaagaa atttcttcta ccattcgctc gattgagcaa gtgttcaagt tggtagacca
    14101 aggtgaaggt ttttatcaag atggatccta tatcgaccat accaatgttg cctatacggg
    14161 tgcttatggg aatgttttga ttgatggcct gtctcaactg ttgccagtca ttcaaaagac
    14221 caagaatcca atcgataaag ataaaatgca aaccatgtac cactggattg ataaatcgtt
    14281 tgctcctttg ctggtgaatg gagagctgat ggatatgagt cgtggacgct cgatcagtcg
    14341 tgcaaatagc gaggggcacg tggccgcagt agaagtacta agagggattc accgaatagc
    14401 ggatatgtct gaaggagaaa ccaaacaacg tttgcagagt cttgtgaaga ccattgttca
    14461 atcggatagt tattatgatg tctttaagaa tttgaagact tataaggata tcagtttgat
    14521 gcaatccttg ttaagtgatg caggagtcgc aagtgttcca agaacaagtt acctatctgc
    14581 ctttaacaag atggataaaa cagccatgta caatgcagag aaagggtttg gatttggctt
    14641 gtcactcttt tccagtcgta ccttgaatta cgaacacatg aacaaggaaa ataaacatgg
    14701 ttggtatacg agtgatggga tgttctatct ttacaatggc gatttgagtc actatagcga
    14761 tggctactgg ccaacagtta atccatataa gatgcctggt acaacagaga cggatgctaa
    14821 gagagcggat agcgatacag gtaaagttct accgtctgct ttcgttggaa cgagcaaact
    14881 agatgatgcc aatgcgacag caaccatgga tttcaccaac tggaatcaaa cattgactgc
    14941 tcataagagc tggtttatgc tgaaggataa gatcgccttt ttaggaagca atatccaaaa
    15001 cacttcaaca gatactgctg caactacaat tgaccagaga aaactggaat caagtaatcc
    15061 atataaagtc tatgtcaatg ataaagaagc ctcccttaca gaacaagaaa aggattatcc
    15121 tgaaacccaa agtgtctttt tagaatcgtc cgattcgaaa aagaatattg gttacttttt
    15181 ctttaagaag agttcaatca gtatgagtaa ggctttgcaa aagggagcct ggaaggatat
    15241 caatgaagga cagtcagaca aggaagttga aaatgaattt cttacgatta gtcaggctca
    15301 taagcaaaat ggagattctt atggctatat gctcattcct aacgtggatc gtgccacctt
    15361 caatcaaatg ataaaagagt tagaaagtag cctcatcgaa aataacgaaa cccttcagtc
    15421 tgtttatgat gctaaacaag gagtttgggg cattgtgaaa tatgatgatt ctgtctctac
    15481 tatttccaac caattccaag ttttgaaacg tggagtctat accattcgaa aagaagggga
    15541 tgaatataag attgcctact ataatcctga aactcaggaa tcagctccag atcaggaagt
    15601 ctttaaaaag ctagagcaag cagctcagcc acaagtacag aattcaaaag aaaaggaaaa
    15661 atctgaagag gaaaagaacc attcggatca aaagaatctc cctcagacag gagaaggtca
    15721 gtcaatcttg gcaagtctag ggttcttgct acttggggca ttttatctat tccgtagagg
    15781 aaagaacaac taatttgttc atcatctaga tgaattacat gaaactgtgg aaaatagaaa
    15841 aaatccgaag aaggccaaat atcttcggat ttttctttta aaaaaggata agttgatact
    15901 tatccttttt tacttcatta ttatgtgact atagcgtttt gaaactagaa tagaacgtta
    15961 taactgctaa aagatttcta taaattcatt tgattttcct aatcaatttg ttcgtatcct
    16021 atttcactcc actatataag tccgtctcgc tccgtgaggt gcgcgtcgaa ggttatacca
    16081 aaaaaactcc aaacgcgata caataaaggt gttcaatcca actgtaaagc gaaaggagaa
    16141 aaaatatggc acaaaagact catagtttat catacacaaa gtggatgtgt tctatcatat
    16201 tgtgttcacc cctaagtata gacgaaaagt catctataat caatatagaa gtagtttagg
    16261 cgaaatattt catcgcttgt gtagttataa aggtgttgaa atgatcgagg gtcacttaat
    16321 gccagaccat gtacacatgt tagtcagtat tccactaagg ctaagtgttt caagttttat
    16381 ggggtattta aaaggcaaaa gtgcactcat gatgtttgac aaacacgcca atctcaagta
    16441 caagtttggg aatcggcatt tctgggcgga aggttattat gtaagtacag tagggcttaa
    16501 taaagccaca cttaagaaat atagtcaaga ttaaagaaat tatccagtgg atgatttctt
    16561 cacgagtatg aaaatttgag aactagtaaa gtatgatata tagtaaaatg aaataagaac
    16621 aggacaaaat caatcaggac agtcaaatcg atttttaaca atgttttaga agtagaggtg
    16681 tactattcca gtttcaatct actatagcac tagataaatt gagtgtaaaa gaatatgagg
    16741 atccctttag ggatagtggt aagtaatacc aaagtctctt aaagaggcaa gtgacgagtc
    16801 aagagcaata aggcttgaac aacgtgaaag ccagcgtctt taggcgctgg ctgatgattt
    16861 gggcttatag ctctgagata aaccacccgt tagacgggta gttatgattt tatctgagtg
    16921 taatatactg ttgggcaatc tcgctgatgc ggtcaaagtt gccttgggaa gcgagtttat
    16981 tgagttcgcc accaattcca acggcatctg caccagcagc gaaccattga gggatgttgt
    17041 ttaggccgac tcctccggtt accattacgg aaacttgtgg gatcggtgcc ttgactgcag
    17101 agatatatgc tggactgaga gtactacctg ggaagagttt gatgatttca ctaccggctt
    17161 caagtgcagt cgtgatctct gtgagggtaa tacagcctgg aatgtacggt gtgctgtaga
    17221 gattgcacat tttcgcagtt tcagcatgga aagatggaga aacaacgtaa tttgctccag
    17281 ctagaatggc atctctagca gttacggcat caagcacagt acctgcaccg atacaaacac
    17341 tctgatcgtc ctgatacaag tctacaagtt ccttgatgat ttgtcctgca tactgattgg
    17401 tataggcgat ttcaatagct ttgataccgc ccttgataca agcaatcgag gcttgcagtc
    17461 cttcttcctt tgtatttcct cgaataacag cgacaatttt ttgttttttt agttcaataa
    17521 tcgtatctga tttggtcatg taattctcct aacgaatgat atcttgtgca tttgccagta
    17581 aattttcaat actagttgcg gaagtggaga gatggtctcc tggaagagtg catttgagag
    17641 ttgcgctcgc aactgcaaag tcaatggtag tttttaggga ggaatgatgg agtagttggt
    17701 aaagggcacc agatataaag gcatccccgc taccaattcg ttgatagact gcagttttta
    17761 gttggacaga ctcttcaaat agttcttcta gagcataggc ttgatagaca tttttgtctt
    17821 gctcatcact agagcggagg gtatggaaaa tggccttgaa accataggct tcttttaaaa
    17881 gtcgcatgcg attttccacc tcttctaggc tagcactgtc tcttggaaac atctctagat
    17941 tttggtcatc aatcatgaga ggatcaatac cgaagcaata gtcagtaaaa cgtgcaaact
    18001 tagaaaattc atacttggct tctaggactg aaatcatctt tgttctcaga ttgagatcca
    18061 ttgaaacgac aattcctcgg cgcttggctt cttccaagag taggagaagg atcgtacgga
    18121 cctcttgacc gatagctacg gtgattccac taaaatgaaa atggctaatc ccctgaaaga
    18181 gagaatccat atctagcatg tttggccgaa tctggctgat actcgtatgc ttacgatcgt
    18241 agaaaacttc actttgacga caaccaaagc cgttctccaa atagtagagg ccgattcgat
    18301 cgccaagccg acagattgaa ctggtatcga tttggtgctg tttcaagaat gtgagaaaac
    18361 gatctccaat ctcgttggca gggagtgcgg taaagacttt cgttgagata cccagggctt
    18421 gcaaattaca agcgatgtta atttctgatc cgccaaaata agtcgaactg gcaacatgat
    18481 cgccgatact ggtggcatct aatggtgaaa ttcgaattaa tggttcgcca aacagtaata
    18541 ttttcgtcat ccattagttc ctttctggtt tagaatagct ttacttggct aatacttctt
    18601 tcaaataagc agcgacttca tcatcttggc agttttcaaa gaagtattct tggaaatatt
    18661 tgccagagat ggtatctttg aggaagtctt ggtcgattgt tttaaggatg gtcatcaaat
    18721 cattgtgggt gatttgtttc acctcgttta agatacgagc gttgcgttgt tcagggatta
    18781 cacgttctct tgggtagcca ccgcccattt cttcatcaaa taagcgttca aacatcaatt
    18841 tgagggtcag ttctgccccc cagccaaatc ctttggcata agggatagac aaggcgttac
    18901 caccattgat ttgagaataa aggtaagcgt cagttgggtc cactgctaga ccacagacaa
    18961 caccagggaa gctgtttaaa gcaagcatag cccctacacc cgtaccacag ccggtaacaa
    19021 caaagtcaac tgcctttgta tttaaaagga tggcagccat tagtccgttc tgcacataag
    19081 ttaattgact ttctccttct tctccacgca taccatagtt aaataattgg tagccttttt
    19141 tatctgtcgc ttcttttaga ctatcgtaaa taatgtgatt cttgctagct tgactatttt
    19201 cattgattaa tgcgattttc ataagatctt ctttctattt ttactcaggt tgttttccga
    19261 tgtaggctaa gataccgcca tctacatata ggatgtggcc attgacaaaa ttgctggcat
    19321 cactagcgag aaagacagca gggcccatca aatcttcagg atttccccaa cgtgcagcag
    19381 gtgtttttgc aatgatgaac tggtcaaatg ggtggcgaga accatcttct tgcaactcac
    19441 gaagaggtgc tgtttgagga gtggcaatat aacccggtcc aattccgtta cattggatat
    19501 tggctccacc gtattcagac gcaatgttgc gggtcaacat tttcaagccc cctttagcag
    19561 cagcataagc gctaactgtt tcacgtccca gttcgctcat catcgaacaa atattgataa
    19621 tctttccatg ccctttcttt atcatagaag gaataactgc ctttgaaacg ataaatggtg
    19681 cgtttaagtc aatatcgatg accttacgga aatcagcggc gctcatttcg cacattggaa
    19741 ctcggcggat aataccagcg ttattaacga ggatgtcaat gacaccaacc tcttgttcga
    19801 tttgcttgac catggcttgg ataccgtcct cgtctgtcac gtcacagaca tatccatggg
    19861 cttcgatgcc aacttcacga taagcttcaa tccctttatt gaccagatct tgattgatat
    19921 cgttaaagac aatagtagcg ccggcttcag cgtaggattt ggcaatagca aatccaattc
    19981 cataagaagc gccggtgatg agagcaattt ttccttttaa agaaaactgc tcaattgaga
    20041 atgatgtatt tgtcatttga tacttccttt caaatttgtt aagacttctc tataaccaaa
    20101 ttatattcta gtaaacc
//
LOCUS       DAVKHZ010000035        19815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000035 DAVKHZ010000000
VERSION     DAVKHZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.035"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            635..1018
                     /locus_tag="VWW99_001153"
     CDS             635..1018
                     /locus_tag="VWW99_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9539001.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            1070..1759
                     /locus_tag="VWW99_001154"
     CDS             1070..1759
                     /locus_tag="VWW99_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539002.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFSLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVLVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1801..2034
                     /locus_tag="VWW99_001155"
     CDS             1801..2034
                     /locus_tag="VWW99_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539003.1"
                     /translation="MFKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            2185..2796
                     /locus_tag="VWW99_001156"
     CDS             2185..2796
                     /locus_tag="VWW99_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539004.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2957..3751
                     /locus_tag="VWW99_001157"
     CDS             2957..3751
                     /locus_tag="VWW99_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9539005.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3748..4383
                     /gene="trmB"
                     /locus_tag="VWW99_001158"
     CDS             3748..4383
                     /gene="trmB"
                     /locus_tag="VWW99_001158"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9539006.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4509..4988
                     /gene="rimP"
                     /locus_tag="VWW99_001159"
     CDS             4509..4988
                     /gene="rimP"
                     /locus_tag="VWW99_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9539007.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            5032..6168
                     /gene="nusA"
                     /locus_tag="VWW99_001160"
     CDS             5032..6168
                     /gene="nusA"
                     /locus_tag="VWW99_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9539008.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6190..6483
                     /locus_tag="VWW99_001161"
     CDS             6190..6483
                     /locus_tag="VWW99_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9539009.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6476..6775
                     /locus_tag="VWW99_001162"
     CDS             6476..6775
                     /locus_tag="VWW99_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9539010.1"
                     /translation="MNKQKISNLLGLAQRAGRIILGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6792..9584
                     /gene="infB"
                     /locus_tag="VWW99_001163"
     CDS             6792..9584
                     /gene="infB"
                     /locus_tag="VWW99_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9539011.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAADPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPYKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9835..10185
                     /gene="rbfA"
                     /locus_tag="VWW99_001164"
     CDS             9835..10185
                     /gene="rbfA"
                     /locus_tag="VWW99_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9539012.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKVQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10464..11243
                     /locus_tag="VWW99_001165"
     CDS             10464..11243
                     /locus_tag="VWW99_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539013.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            11245..11427
                     /locus_tag="VWW99_001166"
     CDS             11245..11427
                     /locus_tag="VWW99_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539014.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11782..11856)
                     /locus_tag="VWW99_001167"
                     /pseudo
     CDS             complement(11782..11856)
                     /locus_tag="VWW99_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11887..12120
                     /locus_tag="VWW99_001168"
     CDS             11887..12120
                     /locus_tag="VWW99_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9539015.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            12120..13454
                     /locus_tag="VWW99_001169"
     CDS             12120..13454
                     /locus_tag="VWW99_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9539016.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13464..13718
                     /locus_tag="VWW99_001170"
     CDS             13464..13718
                     /locus_tag="VWW99_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9539017.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            13814..14209
                     /locus_tag="VWW99_001171"
     CDS             13814..14209
                     /locus_tag="VWW99_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9539018.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            14621..15070
                     /locus_tag="VWW99_001172"
     CDS             14621..15070
                     /locus_tag="VWW99_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9539019.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            15057..15617
                     /locus_tag="VWW99_001173"
     CDS             15057..15617
                     /locus_tag="VWW99_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539020.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKILEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            15617..16195
                     /locus_tag="VWW99_001174"
     CDS             15617..16195
                     /locus_tag="VWW99_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9539021.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            16188..18839
                     /locus_tag="VWW99_001175"
     CDS             16188..18839
                     /locus_tag="VWW99_001175"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9539022.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPVFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            18909..19664
                     /locus_tag="VWW99_001176"
     CDS             18909..19664
                     /locus_tag="VWW99_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9539023.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPCFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
BASE COUNT         5991 a         3662 c         4492 g         5670 t
ORIGIN      
        1 atgcttactt tttaaatttt ttaacaattc aagatgtttc gatgacaatt caagatttgg
       61 atgaaaaatt ttaaaaaaca atgatatact aaacttgtca aagttgcaac gggtgctgct
      121 acattatgtt aaaaatcgct cttgattgat aagctggatt tgttaggaaa ggagtaaata
      181 attatggata agaaaaagat agtttcgact ataatatgta tagtatttct tgtagtgtca
      241 gtagataatt tttttagaga cttaaccccg ttactgttca ttttaaatat tattggcctg
      301 tcttgctttt cagtgctaac atacattaac ataaaagaga tactcttaaa tataagtaaa
      361 tgatgatttg gttagtccta gaaccaatca acttatgttt atcggcttgg cagattttat
      421 gtttgtcatt tgtttgtata gaggtatttc agaaacagaa ttctatcaac aactaatcgc
      481 ttatataggt gtattctctg cttgtttatc acgcttctgc tcatgtgggg cttgaagtat
      541 tataaaaagt agctttctat ccttttctga caggatattt gagtagaagg ctctattaaa
      601 ttgttagtga aataaatgaa tggaggtatt taatatgaaa tacagattat tttttgttat
      661 tttcttgagt agtgtgttgg atattctttt agggacattt ttacaaatct ctatcgtatc
      721 tattgggtgg cttgttcttt acagtggttt gtttgaagca ggagttttcc ttcttgctaa
      781 taaaggggtg gcggtaaaaa tcaaggaagt agatattcga aatcgcttta aatttatttt
      841 tggaaaaacc ttatggtttc aaattctttt gctcatcttt ttgataatca aactttatct
      901 tggtttggat gcgaggttga ttttattcta tggacatatt ttcattgtct ttaatgcctt
      961 aatgtatcta ttatctagta gtcaggttag ccttaaaaaa aacaaactgt cttcttaatc
     1021 ttacagcagt agagcctgac aaggtgaggt catagtaggg aggatttcta tgaaaaagta
     1081 tcaacttcta ttcaaaataa gtgcagtctt ctcttactta tttttcgtat ttagtctttc
     1141 tcagctgacg cttatcgtcc aaaactattg gcaattttct tctcagatag gcaatttatt
     1201 ctggattcaa aatatcttga gtttactttt tattggagtc atgattgtgg ttcttgttaa
     1261 gacaggccat ggttatctct tccgcattcc aagaaaaaaa tggctttggt attcgatttt
     1321 gacagtatta gtgctagtgt tccagatctc ttttaacgtt cagacagcta aacatgttca
     1381 gtcaactgcg gaaggttggg ctgtattgat tggttatagt gggactaact ttgcagagct
     1441 aggtatttat atagccctgt tctttctggt tccactgatg gaagaattga tttatagagg
     1501 attactgcaa catgctttct ttaagcattc gcgatttggt cttgatttgc ttcttccttc
     1561 tattttattt gctctccctc atttttcaag cctgcctagt ctgttagata tcttcgtctt
     1621 tgcaacagtt ggaatcatct ttgctggttt gacccgctat accaagagca tttatccatc
     1681 ctatgcggtg catgtgatta ataatattgt agcgaccttc ccgtttttgc tcacttttct
     1741 acatagggtc ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggaggtcatc
     1801 atgtttaaac acttattttt cctagattcc aaaactttag atcggttgac accctatatt
     1861 ctagtcttgg cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg
     1921 gtggttttta atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc
     1981 tatgtggtcg gattttggtt actaatactc aatgaaaatc aaagagcaaa ctaggaaact
     2041 agccgcaggc tgtacttgag tacggcaagg cgacgttgac gtggtttgaa tttgattttc
     2101 gaagagtata aaatggtttg ttttggaaag gctagaacta aagaatgacg tgtaggaagt
     2161 ctgtttgttg aggaggataa ttttatggag ttttttgata aatttcatgc cttgtgtttt
     2221 ggatttttag tactaataat tgtcattaca gttccttata cgattaacca tgggggtttt
     2281 tttcaaaatg aatctgcatt gattcttgta agtcttcttg taacctcgct gagtgttgct
     2341 tatgctagaa agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgctt
     2401 ttcattgcaa tctttcttct aagtgtactt gagacgctgg tttatattca tttcttcgct
     2461 gtttcttctg gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct
     2521 ttgattttaa ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt
     2581 cttcaaggtg cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt
     2641 tctttcatgc atggaccttc taatgtccct tcgtttattt tttatctact tgggggcttg
     2701 ttgctgggcc ttgcttataa aaagagccaa aacttatggg tttctactct agtccacatg
     2761 ttttacaaca gttggccact cttatattat ttataaaaat tatgaaaagg taagcagaag
     2821 acggtgctta cctttttcaa gctataatga tacccaagcc aatccagagg cttttctttg
     2881 agaatcgggt tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt
     2941 tatacggagg aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg
     3001 gaaaagtggc aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat
     3061 gaacacctct ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg
     3121 gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat
     3181 attgaccccc tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg
     3241 ctcacgtccg ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga
     3301 tttactacag tcttggcagg aaacggctcc agatgctttg cgtaaaaatc attttatcag
     3361 tgaagtgatg gctgatttac gtcagactat tccaggattt agagaggacc atgcgaccat
     3421 tgtccatgga gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt
     3481 agtagattgg gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg
     3541 ccattatatt tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa
     3601 tcaaacggta ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc
     3661 caagtattat atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca
     3721 tttccgagac aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa
     3781 ttactagagg caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg
     3841 cgggacttgt ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc
     3901 tttgtttcag gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa
     3961 aagtctgttt tgagctacgc tttggacaag gtgcttgaag ctggagtgtc taacatcaag
     4021 ctcttgtggg tagatggttc tgacttgact gactactttg aagacggtga gattgatcgc
     4081 ttgtatctga acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc
     4141 tacaagacct ttttggatac cttcaaacgt atcttgcctg aaaatggaga aattcatttc
     4201 aagacggata accgtggctt gtttgagtac agtttagtga gcttttctca atatggcatg
     4261 aaactcaatg gtgtctggct agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca
     4321 gaatacgagc aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt
     4381 taagagatag cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg
     4441 tgctatactg atggtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt
     4501 atgaggaggt ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg
     4561 tcatagaagc tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga
     4621 ttctcagtat ttttgtagat aaacccgaag gaattacctt gaacgacacg gcagacttaa
     4681 cagaaatgat cagtcctgtc ctagacacca tcaagccaga tcccttccca gaacaatatt
     4741 tcctagaaat taccagtcca ggcttggaac gtcctttgaa aaccaaggat gccgtcgctg
     4801 gagcggttgg aaaatacatc catgtcgggc tctaccaagc catcgataag caaaaggtct
     4861 ttgaaggaac cttgttggcc ttcgaagagg acgagttgac tatggaatat atggacaaga
     4921 cgcgtaagaa aaccgtccaa attccataca gtttagtatc aaaagcacgt ttagcagtta
     4981 aattatagaa aaagaaagga tagcttttga ggattcaaaa gtgaagaaaa catgagtaaa
     5041 gaaatgctag aggccttccg cattttggaa gaagacaagg gaatcaaaaa agaagatatc
     5101 atcgacgcag tagtagagtc gcttcgttcc gcttatcgca gacgctatgg tcagtcagac
     5161 agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta cagtttatac tgtccgtgaa
     5221 gttgttgatg aagtatttga tagccgtttg gaaatcagct tgaaagatgc tcttgccatt
     5281 aattcagctt atgaacttgg agacaaaatc aagtttgaag aagcaccagc tgagtttggt
     5341 cgtgtagcag cccaatctgc caaacaaacc atcatggaaa aaatgcgcaa gcaaacacgt
     5401 gccatcactt acaatactta caaagaacat gagcaagaaa tcatgtctgg tacagtagaa
     5461 cgctttgaca accgctttat ctatgtcaac cttggtagca tcgaagccca attgtcaaaa
     5521 caagaccaaa ttcctggaga agtttttgct tctcatgatc gtatcgaagt ttatgtttac
     5581 aaggttgaag acaaccctcg tggtgtgaac gtctttgtta gccgtagcca tccagaaatg
     5641 atcaaacgtt tgatggagca agaaattcca gaagtttatg atggaactgt tgaaatcatg
     5701 agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg ttcgtagcca caatccaaac
     5761 gtggatgcta tcggtacaat cgttggacgt ggtggtgcta atatcaagaa gattactagc
     5821 aaattccacc cagctcgtta cgatgctaaa aatgaccgca tggtaccaat cgaagaaaat
     5881 atcgatgtta tcgagtgggt agcagatcca gctgaattta tctacaatgc catcgctcct
     5941 gctgaggttg accaagttat ctttgatgaa aacgacagca aacgtgcctt ggtggttgtt
     6001 ccagataaca agctttctct tgccattggt cgtcgtggac aaaacgtgcg cttggcggct
     6061 cacttgactg gttaccgtat cgatatcaag tctgctagcg aatttgaagc catggaagac
     6121 gctgcttcag tagagttgga agtagaaaac gatactgtag aagaataaaa gctgctagag
     6181 gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt ctgttgtgtc taacgaagtg
     6241 attgataagc gtgatttgct ccgcattgtc aagaacaagg aaggacaagt ctttattgat
     6301 cctacgggca aggccaatgg ccgcggcgct tatatcaaac tagacaatgc agaagcccta
     6361 gaggcgaaaa agaagaaggt ctttaaccgc agctttagca tggaagtgga agaaagcttt
     6421 tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa gaagagagtt gggacttgaa
     6481 taagcaaaag ataagtaatc tcttggggct tgctcagcga gcagggcgca tcatattggg
     6541 tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc aagttggtct ttctagctca
     6601 tgatgctgga cccaatctga ccaagaagat tcaagataaa agtcattatt atcaagtaga
     6661 aattgtaacc gtgttttcaa cactggaatt aagcatagca gtcgggaaat cgagaaaggt
     6721 tttggctgta acagatgctg gatttacaaa gaaaatgagg tctcttatgg aatagaagag
     6781 gaggacatga tttgtctaag aaaagattgt acgaaatcgc aaaagaactt ggaaaagaaa
     6841 gtaaagaagt tgtagcgcgt gcaaaagagt taggcttgga tgtgaaaagc cactcatcaa
     6901 gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt taagcctgca gctgatccga
     6961 aagtagaagc aaaacctgca gcaccaaaag taagtgcaga aaagaaagcc gaaaaatctg
     7021 agccagctaa accagctgta gctaaggaag aggcaaaacc ggctgagcca gttgctccga
     7081 aaacagaaaa agtagcagtg aaaccgcaaa gtcgtaattt caaggctgag cgtgaagccc
     7141 gtgctaaaga gcaggcagaa cgacgcaagc aaaataaggg caataaccgt gaccaacaac
     7201 aaaacggaaa ccgtcagaaa aacgacggcc gtaatggtgg aaaacaaggt caaagcaacc
     7261 gcgacaatcg tcgctttaat gaccaagcta agaagcagca aggtcagcaa aaacgtagaa
     7321 atgagcgccg tcagcaagag gacaaacgtt caaatcaagt ggctccacgt attgacttta
     7381 aagcccgtgc agcagcccta aaagcagagc aaaatgcaga gtacgctcgt tcaagtgagg
     7441 aacgcttcaa gcagtatcag gctgctaaag aagccttggc tcaagctaac aaacgcaagg
     7501 aaccagagga aatctttgaa gaagcggcta agttagctga acaagcacag caagttcaag
     7561 cagtggttga agtcgtccct gagaaaaaag aacctgcagt ggatacacgt cgtaaaaaac
     7621 aagctcgacc atacaaaaat cgtgacgatt atgatcatga agaagatggt cctagaaaac
     7681 aacaaaagaa tcgaagtagt caaaatcaag tgagaaatca aaagaatagt aactggaata
     7741 acaacaaaaa gaacaaaaaa ggcaataaca agaacaaccg taatcagact ccaaaacctg
     7801 ttacggagcg taaattccat gaattgccaa cagaatttga atatacagat ggtatgaccg
     7861 ttgcggaaat cgcaaaacgt atcaaacgtg aaccagctga aattgttaag aaacttttca
     7921 tgatgggtgt catggccaca caaaaccaat ccttggatgg ggaaacaatt gaactcctca
     7981 tggtggatta cggtatcgaa gccaaacaaa aggttgaagt ggataatgct gacatcgaac
     8041 gtttctttgt cgaagatggt tatctcaatg aagatgaatt ggttgagcgt ccaccagttg
     8101 ttactatcat gggacacgtt gaccacggta aaacaaccct tttggatact cttcgtaact
     8161 cacgtgttgc gacaggtgaa gcaggtggta ttactcagca tatcggtgcc taccaaatcg
     8221 tggaaaatgg taagaagatt accttccttg atacaccagg acacgcggcc tttacatcaa
     8281 tgcgtgcgcg tggtgcttct gttaccgata ttacgatctt ggtcgtagcg gcagatgacg
     8341 gggttatgcc tcagactatc gaagccatta accactcaaa agcggccaac gttccaatca
     8401 tcgtagccat taacaagatt gataaaccag gtgctaaccc agaacgcgtt atcggtgaat
     8461 tggcagagca tggtgtgatg tcaaccgctt ggggtggaga ttctgaattt gttgaaatct
     8521 cagctaaatt caaccaaaat atcgaagaat tgttggaaac agtccttctt gtagctgaaa
     8581 tccaagaact taaggcagac ccaacagtgc gtgcaatcgg tacggttatc gaagcgcgct
     8641 tggataaagg aaaaggtgcg gtcgcaaccc ttcttgtaca acaaggtacc ttgaatgttc
     8701 aagacccaat cgttgtcgga aataccttcg gtcgtgtccg tgctatgacc aacgaccttg
     8761 gtcgtcgtgt taaagttgct ggaccatcaa caccagtctc tatcacaggt ttgaacgaag
     8821 caccgatggc gggtgaccac tttgccgttt acgaggatga aaaatctgcg cgtgcagcag
     8881 gtgaagagcg tgccaaacgt gccctcatga aacaacgtca agctacccaa cgtgttagcc
     8941 ttgaaaacct ctttgatacc cttaaagctg gggaactcaa atctgttaat gttatcatca
     9001 aggctgatgt acaaggttct gttgaagccc tttctgcctc acttcaaaag attgacgtgg
     9061 aaggtgtcaa agtgactatc gtccactcag cggtcggtgc tatcaacgaa tcagacgtga
     9121 cccttgccga agcttcaaat gcctttatcg ttggtttcaa cgtacgccct acaccacaag
     9181 ctcgtcaaca agcagaagct gacgatgtgg aaatccgtct tcacagcatt atctacaagg
     9241 ttatcgaaga gatggaagaa gctatgaaag ggatgcttga tccagaattt gaagaaaaag
     9301 ttattggtga agcggttatc cgtgaaacct tcaaggtgtc taaagtggga actatcggtg
     9361 gatttatggt tatcaacggt aaggttgccc gtgactctaa agtccgtgtt atccgtgatg
     9421 gtgtcgttat ctatgatggt gaactcgcaa gcttgaaaca ctataaagac gacgttaagg
     9481 aagttacaaa cggccgtgaa ggtggattga tgatcgacgg ctacaatgat attaagatgg
     9541 atgatgtgat tgaggcgtat gtcatggaag aaatcaagag ataagatttt ttgctccttt
     9601 cttaggtggt gagggacgca agcaaaccga tggtttcatt gcttattttt gagcctaggg
     9661 tctcaaaaat cccctgtgat gggactgata aatcagttcc atcactttca ccacggcgaa
     9721 agaagcagat gacttcaaat tgaacttcgt ttcaatttaa actgaaaatc aagaagttta
     9781 aaatagctag gtctgctggc ctagcttttg gttcaaagta gagaaaggaa tatcatggca
     9841 aatcatttcc gtacagatcg tgtgggcatg gaaatcaagc gtgaagtcaa tgagattttg
     9901 caaaagaaag tccgtgatcc acgtgtccaa ggtgtgacca tcacagatgt tcagatgctg
     9961 ggtgacttgt ctgttgccaa ggtttattac accattttga gtaaccttgc ttcggataac
    10021 caaaaagtcc aaatcgggct tgaaaaagca actggtacca tcaaacgtga acttggtcgc
    10081 aatttgaaat tgtacaaaat cccagatttg accttcgtca aagacgagtc catcgagtat
    10141 ggaaacaaga ttgacgagat gctacgcaat ctggataaga actaaagaag aggggttgcc
    10201 cctctttttt ggtggaggaa aataggttga atttgaaatg gaaaaatatt cttttataat
    10261 agattgaaac tagaatagta cgcctctact tctaaaatat tgttagaaat cgatttgact
    10321 gtcctgatcg atttgtcctg ttcttgtttc gttttaatat aaaaaaggga ttctgtattt
    10381 tttaatgtta tctaattaga aaatgctttt tttgtaggaa atataatatg ataaggtgta
    10441 aaaaagaaat aaggagtttg tatatggctg aacaagactt agctatgcaa gtattgcaac
    10501 aagtggtgaa actacctgtt gttaaggttg atcgttcgaa atttttagtg gataagtttt
    10561 ccaaagaatt ggatccaaaa gatattccta ccttattgga acaaggtaca acgactcttc
    10621 tatctcaaga aatattagat cgtgtagcta atgcttgtat tcgggacaat gtattattag
    10681 cgagtgggac ttctgttttg gcaggattag ctggagggct tgctatggca attaccattc
    10741 cagctgatgt ggctcaattt tatgctttct ctctgaaatt ggctcaagaa ttaggttata
    10801 tttatggtta tgaggatctt tgggcttcac gcgaggagtt gagtgaagat gctcaaaata
    10861 ccctcttgct ttatctaggc gtaatgttag gggtgaatgg aaccgctgct ttgctacgtg
    10921 ctggtggtat aacaattgcc aaacaggtaa tgaaaacagt gcctaataaa gctttaacaa
    10981 agacgctttg gtaccctatt ttgaaaaaag tcttaagaat atttggtgtg aatcttacca
    11041 agggagggct ggccaaagga atagggaaat ttattcctat cttgggtggt atcatttcag
    11101 gtggtttaac ctttgcaact atgaaaccaa tgggggaaag cttgcagaaa gaattatcca
    11161 agctagtcaa ctatagtgaa gttcaatatc aagaagatgt tgaaacaatc cgaaaagagg
    11221 ctgaaatcat caaaggagag taatatgaat cctatcaaag cttttgctaa aatttatggt
    11281 aattactttt tgaccgtgca aggtgtaaaa gtgatgaaaa cgataaagaa agctgaccat
    11341 gtcgttgttg gtctggggaa actttttatt gccgacaagt taatggatac ggctcggtgg
    11401 ctcattaagc cagaggagag agaatgaaat ttttttggtc ttcttgctat tctttttatc
    11461 aaaccgatta ttgggattgt gaaattcttt tggatgatca tctcttttgc agtccaattg
    11521 ctgttttaca agatagtgtt taagatattg gattggctct ttaaacttat ctagatggta
    11581 atccaagttg cagagaacta gcaggaactc cactgctagt tttttattct ctttccatat
    11641 ggtataatat aagcagtaaa atcattttat actcttcgaa aatctcttca aaccacgtca
    11701 gcttcacctt gcagtatata tgttactgac ttcgtcagtt ctatccacaa cctcaaaacg
    11761 gtgttttgag ctgacttcgt cagttctatc tacaacctca aaacactgtt ttgagcaacc
    11821 tgcggctagc ttcctagttt gctctttgat tttcattgag tattagaaca tacaatggag
    11881 gtcgtcatgg acaatatcat cgatgtgtca attcctgttg cagaagtggt ggacaagcat
    11941 ccagaagtct tggaaattct agtggagttg ggttttaaac cccttgccaa tcccttaatg
    12001 cgcaatacag ttggtcgtaa agtatcactt aaacagggtt ctaagctagc aggaactcct
    12061 atggacaaga ttgtacgcac actggaagcg aatggctacg aagtgattgg attagactaa
    12121 tgacagatga acggattcat atcctacggg atattttgtt agaattgcac aatggcgcct
    12181 ctcctgagtc ggttcaagat cgctttgatg cgacctttac gggcgtgtca gccatcgaga
    12241 tttcccttat ggagcacgag ctgatgaact cggattcggg cgtcactttt gaagatgtta
    12301 tggaactctg tgatgtccat gccaatcttt ttaaaaatgc tatcaaaggt gtcgaagttt
    12361 cagatactga gcatccaggt cacccagttc gtgtcttcaa agaagaaaat ctggctctcc
    12421 gtgcggcctt gattcgcatt cgtagattgt tagataccta tgagtctatg gaagacgagg
    12481 aaatgctggc ggagatgcgt aagggtttgg tgcgtcagat gggacttgtg ggtcaatttg
    12541 acatccatta ccaacgtaag gaagaactct tctttcctat catggagcgc tatggacacg
    12601 attcacctcc caaagttatg tggggagtgg atgatcagat tagggaactc tttcaaacag
    12661 ctctaacgac agccaagtca ctaccagaag tgtcaattag cagtgtaaag gaagcttttg
    12721 aagcttttgc gacagagttt gaaagtatga ttttcaagga agagtccatc ctcctcatga
    12781 ttctccttga gtcttttact caggatgact ggcttcagat tgcggaggag agcgatgcct
    12841 atggctatgc catcatccgt ccgtcagaga aatgggtgcc agaacgacag agctttattg
    12901 aggaaaagat tgcagaggag cctgtacagc tagatacggc agaaggtcaa gttcaacaag
    12961 tcatagatac gccagaaggc cattttacca ttacctttac ccctaaggaa aaggaagctg
    13021 tgctggaccg ccatagtcaa caggcttttg gtaatggcta tctttcagtc gagcaggcca
    13081 atctcatcct caatcacctc cctatggaga ttacctttgt caataaagat gatattttcc
    13141 agtattacaa tgacaatacg ccagctgatg agatgatttt caaacggacg ccgtctcaag
    13201 tcgggcgcaa tgtcgaactc tgccatccgc ctaagtactt ggacaaggtc aaaactatca
    13261 tgaaggggct tcgtgaggga agcaaagaca agtatgaaat gtggttcaag tctgagtcgc
    13321 gaggtaagtt tgtccacatc acctatgctg cagtacacga tgaagacgga gaattccaag
    13381 gagtgttgga gtatgttcag gatatccagc cctaccgtga gattgatacg gactattttc
    13441 gtggattaga ataaggagaa aaaatgagtt acgaacaaga atttatgaag gaatttgaag
    13501 cttgggtcaa tacccaaatc atgattaacg atatggcgca caaggaaagt caaaaagttt
    13561 acgaagaaga ccaggacgag cgtgccaaag atgccatgat tcgctacgag agtcgcttgg
    13621 atgcttatca gttcttgctt ggtaagtttg aaaacttcaa agcaggcaag ggattccatg
    13681 atttgccaga aggcttgttt ggtgagcgaa attattaaac gagaaagatt cttgattttt
    13741 cactaaaatc ttgatagaat gtttatgtta aatccttgtc agagcaggga ttttttattg
    13801 aaaggatttt atcatgtcaa agaaactcaa tcgtaaaaaa caattacgaa atggcctccg
    13861 tcgcgcaggt gccttttcaa gtacggtgac taaggttgta gatgagacaa aaaaagtcgt
    13921 gaagcgtgca gaacagtcag caagcgcagc tggtaaggct gtttctaaaa aagttgaaca
    13981 agcagtagaa gctaccaaag agcaagctca aaaagtagct aattctgtag aagattttgc
    14041 agcaaacttg ggtggacttc cacttgatcg tgccaagact ttctatgatg aaggaatcaa
    14101 gtctgcttca gatttcaaaa actggactga aaaagaactc cttgacttga aaggaatcgg
    14161 tccagctacc atcaagaaat tgaaagaaaa tggcatcaag ttcaagtaat ttttcttgag
    14221 ccttgcattt ccgaaaaaat cttgctacaa tagagccatt agaggtgttt tgaatcccac
    14281 attttacaga aagtggcggc gctgagaagt ccacaaatgt gtcaaaactg gttgctaatg
    14341 actgaaaaaa tgaaatattt ctgtcttttt attttaaaga ctaaagatgc gggcttggcc
    14401 cgaaattggg tggtaccgcg gataaacaca ttcgtccctg tcatgtagat ggcagggctt
    14461 ttcttttgtg tcttagtcaa aggaggttgt tatgaagcaa tcttttaaca caagtaaact
    14521 atattatggt tttcctgttt ttattttagg atatcaagat tagaattttg gctataacat
    14581 cacgacctgt agttcctctt atagtctagg agattgggtt gtgattggag tcgttgcgag
    14641 agagaatgcc gcagagcaga tcaaacagta tcaaaaattt actgtgaata tttctgatga
    14701 aacttctatg cttgcgatgg agcaggctgg ttttatcagt catcaggaga aattggaacg
    14761 tttgggagtg cattatgaaa tttctgaacg aactcagatt cctattttag acgcctgtcc
    14821 acttgtttta gattgtcggg tagataggat tgttgaggaa gacggtattt gccacatctt
    14881 tgccaagatt cttgagcgac ttgttgcccc agaactcctg gatgaaaagg gacattttaa
    14941 aaatcaactg tttgccccaa cctatttcat gggagatgga tatcagcgcg tttatcgcta
    15001 tctggacaag cgtgtagata tgaagggcag tttcatcaaa aaagcgagga agaaggatgg
    15061 caagaactga gctgccagat aaaatcgaaa cagaacgtct cgttttacga gtccgtactg
    15121 tggcggatgc tgaggatatc tttgactatg ctagtttgcc agaggtcgcc tatccagcag
    15181 gttttcctcc agtcaagatc ttggaagatg agatttatta tctggagcac attcttccgg
    15241 agcgtaatca aaaggaaaat ctcccagcag gctacgggat tgtcgtcaaa ggaacggata
    15301 agatcgttgg ctctatctat ttcaaccatc gccatgaaga tgatgtgatg gaaatcggct
    15361 ataccttaca ctcagactat tgggggcgag gttatgtgcc agaagctgcg cgtgccttga
    15421 ttgacttagc ctttaaagat ttgggtcttc acaagattga actaacttgc tttggatata
    15481 accttcaaag tcaacgagtc gcggaaaagc ttggctttac cctcgaagct cgcataagag
    15541 accgcaaaga tgctcaaggc aactgctgtg atgatttaag atatgccttg ctgaagagtg
    15601 aatgggaggt gatttgatgc atcttttcat cattggtgct ccagcatcag gaaaaatgac
    15661 cattggtcaa gaattatctc gactgacgga tgctacccta ttttataacc atcaagccat
    15721 cgattttgca ctagaaatct atcaggacta tacagaggaa atgtgggaat ttgttcgtgg
    15781 aatcactttt tcattccttg gagcgagtgc aagaaatcag cgatcagtaa ttttaacaga
    15841 tgtaattgat ttttcaaatc agtatcatct gatatattta aagcaaattc agagtttgtt
    15901 ggatgattat catcaagaga ttctttttgt tgagttggaa acaagtcttg aagagtgcat
    15961 acgtcgaaat cgaacggaga atcgattaaa gtacaaaccc ttaaaacgac atattgaggt
    16021 atctgaaaga gaaattatag agaccgctga aacacttcaa ttaaattccc aatatcaacc
    16081 gaatgagttg caccactact ttaaaataaa taatacgaat ttgtctgcag aagaagctgc
    16141 taagcaaatt caaaataaaa taaacaaaat agagaaagga cacacacatg tctaaagaac
    16201 tttcatctaa atacaatcca gccgaggttg aggctggtcg ttaccaaaaa tggcttgatg
    16261 ctgatgtttt caagccttca ggcgatcaaa aggctaagcc ttattcaatc gttattccac
    16321 caccaaacgt tacaggtaaa cttcaccttg gtcacgcttg ggatacaact ttgcaagata
    16381 ttatcatccg tcaaaaacgc atgcaaggtt ttgataccct ttggcttcct gggatggacc
    16441 acgcagggat tgccactcag gctaaggtag aggagcgctt gcgtggtgag ggcatttccc
    16501 gctatgacct tggtcgtgag tctttcttga cgaaagtctg ggaatggaaa gacgaatatg
    16561 ccactactat caaggaacaa tggggcaaga tggggctctc tgtagactac tctcgtgagc
    16621 gtttcactct tgacgaaggt ttgtcaaaag ctgttcgtaa ggtctttgtg gacctttaca
    16681 agaaaggctg gatctaccgt ggtgagttta tcatcaactg ggacccagca gctcgcacag
    16741 ccctttctga tattgaggtg attcacaagg atgtagaagg tgccttctac cacatgaatt
    16801 acatgctgga agatggttca cgcgcccttg aagttgctac aactcgtcct gagaccatgt
    16861 ttggggacgt tgcggttgcg gttaatccag aagacccgcg ctacaaggac ttgattggta
    16921 aaaacgtcat ccttccaatc gctaataaac tcatcccaat cgttggagat gagcacgcag
    16981 atcctgagtt tggtactggt gtcgtgaaaa tcacacctgc ccacgatcca aatgacttct
    17041 tggttggcca acgtcataac ttgccacaag tcaacgtcat gaacgacgac ggaactatga
    17101 atgagcttgc ctttgaattt tcaggcatgg atcgttttga agctcgtaag gcagtcgttg
    17161 ctaagttgga agaaatcggt gcccttgtca aaatcgaaaa acgtgtccac agtgttggtc
    17221 actcagaacg tacaggtgtc gtagttgagc cacgcttgtc tactcaatgg ttcgtcaaga
    17281 tggaccaatt ggctaagaac gccattgcca accaagacac agaggacaag gtcgaattct
    17341 acccacctcg tttcaacgat accttccttc aatggatgga aaatgtccac gactgggtta
    17401 tctctcgtca gctttggtgg ggtcaccaaa tccctgcctg gtacaatgct gatggtgaaa
    17461 tgtatgtcgg cgaagaagct ccagaaggtg acggatggac tcaggacgaa gacgtcttgg
    17521 atacttggtt cagttctgcc ctctggccat tttcaaccat gggctggcct gaagtcgact
    17581 cagaagactt taaacgttat ttcccaactt caaccttggt aacaggttac gacatcatct
    17641 tcttctgggt gtctcgtatg atcttccagt cattggaatt cacaggccgt caaccattcc
    17701 aaaacgtcct tatccacggt cttattcgtg acgagcaagg acgcaagatg tctaagtctc
    17761 tcggtaacgg gattgaccca atggatgtta tcgagaaata cggtgccgat gcccttcgat
    17821 ggttcctttc aaacggttct gcgccaggac aagacgtgcg tttctcttat gagaaaatgg
    17881 atgcttcatg gaactttatt aacaagattt ggaacatttc tcgctatatc ctcatgaaca
    17941 atggaggttt gacgctggat gtggcgcatg acaatgtcac aaaagttgca acaggtgagg
    18001 ctggtaatgt gacggaccgc tggattctcc acaatctcaa cgaaaccatt gcaaaagtta
    18061 ctgaaaactt tgataagttc gagtttggtg tggctggtca tatcctttac aacttcattt
    18121 gggaagaatt tgccaactgg tatgttgagt tgaccaagga agttctttac agcgacaatg
    18181 aagacgataa ggtaattact cgatctgttc tcctctatac gctggataaa atccttcgtc
    18241 tccttcactc aataatgccg ttcgtgacag aagaaatctt tggtcagtac gctgaaggct
    18301 ctatcgtgac agcagcatac ccaactgtca acccagtctt tgaggacctt gctgcccaca
    18361 ctggtgtgga aagtctcaaa gacttgatcc gtgccgttcg gaatgcgcgt gcggaagtaa
    18421 atgtagcacc aagcaagcct atcaccatcc ttgttaagac aagcgatagt gacttggaag
    18481 ccttctttaa cagcaatgtc aactatatca aacgctttac aaatccagaa cacttggaaa
    18541 tcgcatcaac catccctgca cctgaactgg ctatgtcaag cgttattaca ggagccgaaa
    18601 tcttcttgcc actggccgat ctcctcaatg ttgaagaaga actagctcgt ctcgacaaag
    18661 aactcgctaa atggcaaaaa gaactggaca tggttggtaa gaaactctct aacgaacgct
    18721 tcgtcgccaa cgctaaacca gaagttgtcc aaaaagaaaa agacaaacaa gccgactacc
    18781 aagccaagta tgatgtgacc gtagcacgta ttgatgagat gaagaagttg gtgaaataaa
    18841 cacagaaaca cggtgatgag ccgtgttttt tggtataatg aaatcaatat ctattgatgg
    18901 agaaagtgat gtccaaacac cctcattatg aattgttaaa tttaattggc tacggtcttg
    18961 ccaagtttga caagcttttt ataaaagaat ttcaatgctt tagcaagtcg gagttttatc
    19021 gctatgtggt ttctctggga attgctgaaa caactggagt tgtaaaaaat agaatggatt
    19081 tatttgatcc ttgttttgac aataatcgaa aaggttggtg gcagaaagct gaagtttatc
    19141 gttttcgtaa agatttaatt gatatgatgt ttggaaatga agatgttcat agttatgctg
    19201 aaatagttaa aatgttactt gccagtgaag ggaagaaaac aggcataacc atcgttgaaa
    19261 aaccaatagt tcgaactaaa ttcaaacgtc tacaggaaac gggaatggag gctgagaatt
    19321 attttatcct tcattttgat aaagaagaaa aattccaagg tggacagtta accgatgccc
    19381 gtctttatgg tgatggatat gactttcagg tagatgtcca agagtattct taccttgccg
    19441 aagtaaaagg tatccgaaaa tctaaggggc gtgtgcgttt aactgcaaaa gaatttgaaa
    19501 aagtcaagga gtttcaatcg gattttattt tatccttggt cacaaatcta gatgatattc
    19561 caaagttagt gttaatcgat aatcctttaa aacattttga gtttaaaaag aatattatca
    19621 aaaatgaaat cattgaatat agaagcgtag aggatttgta ttagttcccc cttaactttt
    19681 ccagttgaag gaacgagaag tcttgacagg tcaacgactt aatgaactgg aaatcaacgg
    19741 tattcgtttg actaagtttt aagatggcga aatcggtatt gaatttatca gtagtgctga
    19801 ttataactct tttat
//
LOCUS       DAVKHZ010000036        19499 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000036 DAVKHZ010000000
VERSION     DAVKHZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.036"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(127..1614)
                     /locus_tag="VWW99_001177"
     CDS             complement(127..1614)
                     /locus_tag="VWW99_001177"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9539024.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(1763..2503)
                     /locus_tag="VWW99_001178"
     CDS             complement(1763..2503)
                     /locus_tag="VWW99_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9539025.1"
                     /translation="MAKLKIDVNDLHKYYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(2503..4668)
                     /locus_tag="VWW99_001179"
     CDS             complement(2503..4668)
                     /locus_tag="VWW99_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9539026.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTIGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            4841..5794
                     /locus_tag="VWW99_001180"
     CDS             4841..5794
                     /locus_tag="VWW99_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539027.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSSLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLVLFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            5871..7859
                     /gene="uvrB"
                     /locus_tag="VWW99_001181"
     CDS             5871..7859
                     /gene="uvrB"
                     /locus_tag="VWW99_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9539028.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            7869..8432
                     /locus_tag="VWW99_001182"
     CDS             7869..8432
                     /locus_tag="VWW99_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539029.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            8398..8918
                     /locus_tag="VWW99_001183"
                     /pseudo
     CDS             8398..8918
                     /locus_tag="VWW99_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8971..9438
                     /locus_tag="VWW99_001184"
     CDS             8971..9438
                     /locus_tag="VWW99_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9539030.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPDTNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLRS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYRHRTEDDWEKKIF"
     gene            complement(9435..9950)
                     /locus_tag="VWW99_001185"
     CDS             complement(9435..9950)
                     /locus_tag="VWW99_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9539031.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALEQLGYLVKD"
     gene            complement(9962..10489)
                     /locus_tag="VWW99_001186"
     CDS             complement(9962..10489)
                     /locus_tag="VWW99_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9539032.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(10771..11334)
                     /locus_tag="VWW99_001187"
     CDS             complement(10771..11334)
                     /locus_tag="VWW99_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9539033.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(11318..11869)
                     /gene="coaC"
                     /locus_tag="VWW99_001188"
     CDS             complement(11318..11869)
                     /gene="coaC"
                     /locus_tag="VWW99_001188"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9539034.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(11881..12570)
                     /gene="coaB"
                     /locus_tag="VWW99_001189"
     CDS             complement(11881..12570)
                     /gene="coaB"
                     /locus_tag="VWW99_001189"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9539035.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQTKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            12800..14470
                     /locus_tag="VWW99_001190"
     CDS             12800..14470
                     /locus_tag="VWW99_001190"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9539036.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            14798..15973
                     /gene="mutY"
                     /locus_tag="VWW99_001191"
     CDS             14798..15973
                     /gene="mutY"
                     /locus_tag="VWW99_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9539037.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            16028..16732
                     /locus_tag="VWW99_001192"
     CDS             16028..16732
                     /locus_tag="VWW99_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9539038.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            16725..18074
                     /gene="vicK"
                     /locus_tag="VWW99_001193"
     CDS             16725..18074
                     /gene="vicK"
                     /locus_tag="VWW99_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9539039.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            18076..18885
                     /locus_tag="VWW99_001194"
     CDS             18076..18885
                     /locus_tag="VWW99_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9539040.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            18976..19218
                     /locus_tag="VWW99_001195"
     CDS             18976..19218
                     /locus_tag="VWW99_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9539041.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            19220..19474
                     /locus_tag="VWW99_001196"
     CDS             19220..19474
                     /locus_tag="VWW99_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9539042.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
BASE COUNT         5822 a         3526 c         4301 g         5850 t
ORIGIN      
        1 cattataggt catatgggac tttttttcta cacaaaaata ggctccataa tatccatagg
       61 ggatttaccc actacaaata ttatagagcc caaatcctgc aaggcataaa cttgcaggaa
      121 atttttttat tcgaaacgac catcttgacg ataggcgata tctggttgcc aagtccattt
      181 ggcaccgaat ttttcaagta ggtcaaagct ggcttgaggt cccatgcttc cagctttata
      241 gtcatgaagt ggggcaccat tttcagccca gagctcttca atacggtcaa tcaacttcca
      301 tgacgcacca acctcatccc agtggctaaa gttagttgag ttgttattta ggacatcata
      361 aatcaatttt tcgtatggtt ctggagaagc accagttgca gtcgcatctg tacggtaatc
      421 aagtgagtta ggagccaagt taaattcttc tcctacttgc ttcccattta ggctaagaga
      481 gaagccttct gttggttgaa tatagatggt caaaatattt ggagcaagtg gttctccaaa
      541 gatagaatcc atttgtttaa agacgatgtt gacatgagtt cctttttcag tcagtcgttt
      601 acctgtacgg aaaaagaaag gaacactacg gaatcgatcg ctgtctacaa agaaggcacc
      661 agatgtaaag gtttcagttg ttgattctgg attcacattt ggctcgctac gataagagat
      721 gtatttcatg ccatcaatct taccagagcg gtattgccca cggataaagt gttctttgag
      781 ttcttcatca gttggatgat agaggttttt aaagacctta atcttttcag cacgaatctc
      841 gtcttttgtg aagcttgctg gtttgtccat ggcgaggagc gaaagaagtt gtagagtgtg
      901 gttttggacc atgtcacgga gggcaccgga ttggtcatag tagccaccac gttcttctac
      961 acccaagcgc tccgcaaagg taatttgaac attgtcgata aaatccttgt tccaaacgtt
     1021 ttcaaaaatc aagtttgcaa agcgaactgc aaagatgctt tggatcattt ccttaccaag
     1081 ataatggtcg atacggaaaa tttgttcttc gtcaaatgtt gctaggagtt cgtcattcaa
     1141 cttgcttgca gttgcgtaat ctgtaccaaa tggtttttca acgatcaagc gctcaaaacc
     1201 tttgccatcg acaatgtttt cagatttgag gtgtttggca atggttccaa agaactgagg
     1261 tgccatagac aagaagaaga gcttattgtg ttcagcttgg tatttttcat taagctcagc
     1321 ttgtaattga cgcaaagcaa tataatgttc cgaatcattg acatcatggc tttgatagta
     1381 gaagtggcta gcaaattctt gggcttgctc ggtactatct gccaaatcaa ggatggactc
     1441 gacaactaca gattcaaaat attccttact ccaaggtcta cgggcagttc caataacggc
     1501 aaagtgcttg gaaagattgc cggattgata tagtctaaaa agggaagggt agagcttgcg
     1561 tttagccagg tctccactcg caccgaaaat tgtaacaata accttagatg acatctagct
     1621 acctaatttc tatttttatt cctagtcttg ctaggtatga ggaaacctca tttcataaac
     1681 ttaattctaa cataattttc cgaaaaatca aaaattccaa tacgaggtag aaaaactgca
     1741 aggaaatcct tacagtttga gtttagacgt ttaagacctt atctaagaac tctttcagac
     1801 gagggtgttg tgggttatca aagatttggt caggtgttcc gtcttcaagg aactcgccat
     1861 ctgcagtaaa gataacgcgg ttggcaacct gacgagcaaa tcccatctca tgggttacga
     1921 taatcatggt catgccttgc tcagccaatt ccttcataac gttaagtacg tctccaacca
     1981 tctcagggtc aagggcagaa gttggttcat cgaagagcat gatgtctgga ttcattgcta
     2041 ggccacgagc gatggccaca cgttgttttt gaccacctga taggctatct ggattggcat
     2101 tagctttatc tgctagtcca accttttcaa gcaactccat tcccaattcc tcagcttctt
     2161 ccttagtcat caacttgtgc tcaataggag caaaggtgat gttgtccaat acagacatat
     2221 gagggaagag gttgaagtgt tggaatacca tgccgatatt ttcacggacg tggtcaacat
     2281 tggttgtttt ttcagttaaa tcatagccgt tcacagtgat gtgaccgcta gtgacttctt
     2341 ctaaaagatt gaggctacgg aggaaagttg acttaccaga acctgaagga ccgatgatac
     2401 aaacaacatc tccttcatag aacttagtcg taattccttt taggacttca ttttttccat
     2461 agtacttgtg taaatcattt acatcaattt ttaattttgc cattaacgaa tcctcttttc
     2521 taagcgtttc gctagtctag tcaaaagtgt gataattaca agatagaaga tagcaaggat
     2581 tgcatacatc ttgaaacttt ggtagttacg agcaatgata atcttaccag tttggaagag
     2641 ttcaactaaa ccgatagcag atacgatagt tgtatcttta agagcgataa cgaattggtt
     2701 gacaaagttt ggcaacatca atttagttgc ttgtggcaag ataatcttac gcatggtttt
     2761 tccataagag atacccaagc ttcggctggc ttccatttgg ccaactggaa cggcctgaat
     2821 accaccacga acgatttcag cgatataagc agccgcattg agtgagaggg caattgttcc
     2881 agctacaaag tcgttaattg ggctttgttg gcctgtgata gactcgatga agtttggaat
     2941 tccccagaag atgaaggctg caagaatcat caatggaata ccacgaataa cgtcaacgaa
     3001 aatctcagag atgacgcgaa gagatttgta tgggctaacg ctaaacatac cgaagataat
     3061 tccgatgaca atggcaatag caaatgagat aagagctaga gcaagagtga taccaagacc
     3121 gctaaggagt tgtttgtagt tgttttgaag caagccccag agcgttgttt cgtcaacagt
     3181 acttgttgaa gcagttgaag attcgctagc taggtatttg tcaagaatct tttggaattc
     3241 accgtttgct ttaaggtttg caagtccgtt gttgaacatt tcaatcagtt ctggatttgc
     3301 tcctttttta acggcaaagg ctgtttcacc gattggagtt ccagagattg gagttttcaa
     3361 tttttgacct tggctgatag aatatttgag aacaggttca tcatccataa cggcatcaat
     3421 ggcaccagtg tttaaactgt catacattga agaaccatca gcaaaggttt tgattttgta
     3481 accgtatttg ctttgatttt ctgttaggaa ggtttgagaa gcagttccgt ttttaacacc
     3541 gattgtcttt ccttttagat cttcataaga agcaatattg cttgattctt tgacaccaag
     3601 aatggtatta gcagtgtagt atgattctga gaagtcaaaa gttgccttac gagcatctgt
     3661 gacagacata ccagcaatga taccatcggc ttgaccagct tggacagcac tgatagcagc
     3721 atcaaaacca gggttggtga tttcaatttc aaaaccttgg tctttagcga ttgccttaat
     3781 caattccata tcaataccag tgtattggtt gcttgaattt tggaaaacaa aaggtgcaaa
     3841 agaagaatcg ctggcaatga tatatttagc cttaacagga atagctttta atcctgcgag
     3901 agtagttgta gttggcactg ctgaagatga tgaagcagtc catttcttga taattttatc
     3961 aagactacca tcttttttca tttcagccaa ggcttggtta aattcagtaa ccaggtgttc
     4021 gtatttactt ccttttttca caccgaaagc aaaacttcct acagcttcac catccatttc
     4081 aatatggagg tcttgacctt ggttaatggc atattcgata acaggtttgt catccatcat
     4141 ggcatcgatg gcaccagcac tcaagctgtt gttcattaaa tcaccagtgt caaatgtttt
     4201 aatagtaaag ccgtatttat ctttgattgt ttcaaggaaa cgttgagcgg cagttccgtt
     4261 tttaacacca acggttttgc cagttaattg gtcgtacttg ctaattttgt gtgactttgt
     4321 agtagcaatg acaacttttg tatcatagta agtatcagac atggtgaaga ctttttcacg
     4381 ttctttagtc tttgtcatcc ctgccatgat agcgtcggct tgcccagctt gaaccgcatt
     4441 gactgctgcg tcaaatccag gataggacat ctgaatgttc cagcctttaa tctcagcgac
     4501 tttgttaata atgtcaacat caattccttt ataagtttga tctgaatctt taaactcaaa
     4561 aggtgcataa gcggtatcag aaacaatctt aatcgtttct gctttggcaa tacctaatga
     4621 gaaaattggg aataaaatta gcaaaaatgc tagaaatttt ttcttcattt ttagtctcct
     4681 tttccgaata ttctctcatt atatcagaaa aagtaaaaaa aggcaaattt ttataatttt
     4741 cgtgtcagaa aatataacat tgatttttcc ttttctttct tgaattgcta ttaaaaagtg
     4801 ctataataat agtatatata gaagaaaaga ggacggggat atgaagaaca aaagaatatt
     4861 taaagacttc caagcttcaa aaatgaattt aaacatttac acaagctcct tgttagcctt
     4921 tgtttttgtc ttcataggag agtttgtggc ttatacttta tatggtatta gtttgttagc
     4981 tctcatcgga cttgctagaa attttggaga ggctggtcaa aatcttgcaa gctacttgca
     5041 gaccttgcat cagagcttga cggataaaac aagtgatttt cgtttaattt taggattact
     5101 ggcctttggt tttattctta acactgtgtt cagatggaca agaaaagttg agaaaagacc
     5161 tattcgaacc ttgggatttt atagagagaa ttgcctcagc aatcttctga aaggatttag
     5221 tctaggcctg gtactttttc ttctgacctt gttaggtttg gtggtcttag gtcaatatcg
     5281 tttggaatcc attcacttga atccttattc tcttgccttt gtcgtcttta ctatcccatt
     5341 ttggatttta caggggacag cagaagaagt ggtggcccgt gcttggctac ttcctcaatt
     5401 ggcctcaaga accaatctaa aactagctat tcttatatct agcctgttct ttaccctgct
     5461 tcatatgggc aattctggcc tcacccctct atctctagta aatctctttt tattcggagt
     5521 tgccatggct ctttaccttc tcaaaactga tacagtttgg ggtgttgcag gtattcatgg
     5581 tgcttggaat tttgctcagg gtaatctctt tgggatttta gttagtggtc aaccgtcagg
     5641 aacgtctctg atgacctttt taccacaagg caatcaagat tggctatcag gtggttcttt
     5701 tggcatagaa ggttccatta tgacaagtct ggtattacta ctgctgattg tctatcttgc
     5761 taataaatta aagaaagaaa atgaaaggat gtgacttcgg tccgtccttt tcttcgtgaa
     5821 aatactataa gtatgctaaa ataggaatag cacatggaga gaggattctt atgatcaatc
     5881 acattacaga taatcaattt aaactagtat caaaatatca accatcagga gatcaacccc
     5941 aagctatcga gcagttggtg gataacattg aggggggaga aaaagctcag attctgatgg
     6001 gggcgactgg aacagggaag acctatacta tgagtcaggt catttctaaa gtcaataaac
     6061 caactctggt tattgcccac aataaaactc tggctggtca gctctatggg gagtttaagg
     6121 aatttttccc tgaaaatgca gttgagtatt tcgtatccta ctatgattat taccagccag
     6181 aggcctatgt cccttctagc gatacctata ttgagaagga tagttctgtc aatgacgaga
     6241 ttgacaagct tcgccactca gctacctcag cccttttgga gcgtaatgat gttattgtcg
     6301 tggcctcagt ctcttgtatc tatggtttgg gttcgcccaa ggaatacgct gatagtgtcg
     6361 ttagtctccg tcctggtcta gagatttctc gtgataaact cttgaatgac ttggtcgata
     6421 ttcagtttga acgtaatgat attgatttcc aacgcggaag atttcgcgtt cgtggggatg
     6481 tggtagagat tttcccagct tcccgagatg aacatgcctt tcgagtagaa ttttttggag
     6541 acgaaattga ccgtatccgt gaggttgagg ctctgacagg tcaggtattg ggagaggtgg
     6601 atcatttggc gattttccca gctactcact ttgtgaccaa tgacgaccat atggaagttg
     6661 ccgttgccaa gattcaagca gaattagaag agcagttagc tgtctttgag aaggaaggta
     6721 aattgcttga agcccagcgc ttgaaacaac ggacagagta cgatatcgaa atgctgcgcg
     6781 agatgggcta tacaaatggg gttgaaaatt attctcgcca catggatgga cgtagtgaag
     6841 gtgagcctcc ttatacgctt ctcgactttt tcccagatga ttttttgatt atgattgacg
     6901 agagtcatat gactatggga caaataaaag gcatgtacaa tggagaccgt tcgcgtaagg
     6961 aaatgctagt aaattatggt ttccgtttac cgtctgcctt ggacaatcgt cctctccgtc
     7021 gtgaggagtt tgaaagccat gtgcatcaga ttgtttacgt atcagcgaca cctggtgact
     7081 atgaaaatga acagactgag acggtgattg agcaaatcat tcgaccgaca gggcttttgg
     7141 atcctgaggt ggaagtccgt ccgactatgg gacagattga tgatctctta ggtgaaatca
     7201 atgcccgtgt tgaaaagaat gaacgaacct ttatcaccac cttgaccaag aaaatggcag
     7261 aggacttgac cgactatttc aaggaaatgg gcatcaaggt caagtacatg cactcggata
     7321 tcaagacctt ggaacggacg gagattatcc gtgatctccg cttgggtgtc tttgatgtct
     7381 tggttggaat caatctcctt cgtgaaggga ttgacgttcc tgaggttagc ttggttgcca
     7441 ttctcgatgc tgacaaggaa ggtttccttc gtaacgagcg tggactcatc cagaccattg
     7501 ggcgtgccgc ccgtaacagt gaaggtcatg tcatcatgta tgcagatacg gttacccagt
     7561 ctatgcaacg tgctatcgat gaaactgccc gccgtcggaa aattcagatg gcctataatg
     7621 aagagcatgg tatcgtacca cagacaatca agaaagaaat ccgtgacctg attgccgtga
     7681 ccaaggcagt tgctaaggaa gaggacaagg aagtcgacat caatagcctc aacaaacaag
     7741 agcgcaaaga actcgtcaag aaactagaaa aacaaatgca agaagccgtc gaagtgcttg
     7801 actttgaact ggcggctcag atccgtgata tgatgttgga agtcaaggcc ttggattagg
     7861 ggaatagtat gatttattta agaaagttaa agagagaaga tttgatgtct ttatgggaaa
     7921 tggcttattc acaacttaat ccagtttgga aacagtatga tgctccctat tatgatgatt
     7981 atcagtattt ttcaaatttt aaagaattcg aactacaaaa atcagaatcc attttaagca
     8041 actcaaatcg ccttggtatt tttgttgatg ataaactagt tgggactgtt tcgcgttatt
     8101 gggtatgtaa agaaacaaga tggatggaat tgggaattgg tatttatgat aaaaaattct
     8161 ggaacactgg tattgggaaa gttgctatgt tgcagtggat agataggacg tttcaggatt
     8221 acttggagtt ggagcatctg ggtttgacaa cttggtcagg gaatcttggt atgatgaaac
     8281 ttgctgaaaa attaagaatg aaaaaagaag ctcatattcc aaaagttcgt tattatcaag
     8341 gtaaatattt tgatagtatt aaatatggta ttttgagaga agactgggag aaaataaatg
     8401 acggttatta tcaaatcaat ggaaactcct aaagagatag aaggtaaatc ccttgttcac
     8461 tggcaaatgt ggagagaggc ttatgatgac cttttgcctg cagaatttca ggagacaatg
     8521 acattagaaa gatgtcgact ctttagtcaa aagtatccag aaaatacatt gattgcgata
     8581 ggtgatctga aggtagttgg ttttatcagt tatggtaact ttcgtgatga gactattcaa
     8641 gctggtgaaa ttattgcttt atatgtttta aaagactatt acggaaaagg catagcacaa
     8701 aagttaatga aagcagcttt gactgctctt aatcattttt ctgaaatttt cttatgggta
     8761 ttgaaagata acaagcgcgc cattgctttc tatcaaaaaa tgggttttac ttttgatgga
     8821 caagtgatgg acaagaaaag atacttgaac ttggaaagcc tataaaggaa aaacgaatgg
     8881 tattctattc taaaataatt ttaaaaagta gaagctaatg gcactggtac caagtctgaa
     8941 aatttaataa attagaaagc gagtaaattt atgtcccgtt cccaattaac gattttaacc
     9001 aatatttgtc tgattgaaga cttagaaact cagcgcgtag tcatgcagta tcgctctcct
     9061 gacaccaatc gctggtcagg ttatgccttt cctggaggtc atgtagaaaa tgatgaggct
     9121 tttgcggagt ctgtcattcg tgaaatctat gaagaaacag ggttgactat ccaaaatcct
     9181 caacttgtcg gcattaaaaa ttggccacta gatacaggtg ggcgctacat tgttatttgt
     9241 tataaggcga ctgagttctc tggtaccctt cgctcttcag aagagggaga agtttcttgg
     9301 gtgcaaaaag accagattcc aaacttggat ctggcctatg atatgctacc tctgatggag
     9361 atgatggaag ctccggacaa atcagagttt ttctaccgtc atcgtacaga agacgattgg
     9421 gaaaagaaaa ttttctaatc ctttactaaa taaccgagct gctccaaggc ctcctcgata
     9481 tagtggaggt cttgttgtgt ttcggcttcg actaggtggt aatggatgcc atctgtcaat
     9541 tcagatagag gtctaaagtc agaatgctcg acttgttcta gaaaatgttg cacatctctg
     9601 cgacaagaca gtttaagcaa ggtttcaatt tctccataaa cgggatggtc aatcaaggta
     9661 ttctggacat gcccaccatt atcgacaata gcaaggagtt cctgaccgat ttcttcaact
     9721 tcatgtttca ctttgaaaag tttgtgaaca taaggattgg tatcaatttg cttatagatg
     9781 tagccacgat tggtggatag gataggagcg ccatcggctc tcaaaattgc aatgtcctgc
     9841 acaatgacct gacgtgtgac atgaaaatgt tccgccaaac tttggccatt aagggcttta
     9901 ggagcttctt tcaagagttg gagcagggct tgtttgcgat cttttgtcat agcttttcct
     9961 tttaacggcg ttttcgaagc actttataga cagctagtgc taatgtatag tctaccatac
    10021 tatggataat tgtgccaaat ccaactagca caaatagaac atagaacatg ttttctacat
    10081 tggtacccga agttgcgtaa aaaaggacac aggctaatac ttcagcaagg gcatgaacaa
    10141 cagccaaaac aaagttgaaa acccaggaag attttggttt atctagggta tcggggaatt
    10201 tttgtaggta aagagctcct aaagcaccaa aagatatatg ggaaaaagcc cgaaaaacga
    10261 taaccatggg atagccagcc atcaaaaatc caaaactaga ggctaggatg acaaaaactg
    10321 ccatcaaggg cgacaagaac atggctataa aaatagcgat gtggctcccc aaagtatagg
    10381 aagcaggtgg aatgacaatc ttgaaaggca taacaattgg aatcaaaatc gcaatagccg
    10441 ttaaaagggc tgtcattgtc ataaattgtg tctttttccg tgtattcaca agaatctcct
    10501 ttttaactgc atatacacta gtatggtaca ataaaccaga caataaagca agaatttact
    10561 tgggtttata gatcattttt tagttaaaag ttatagtaga ttgaaactag aatagtccac
    10621 ctctacttct aaaacattgt tagaaatcga tttggctgtc ctgatcaatt tgtcctgttc
    10681 ttatttcgtt ttactatagt aaagatttca ttaaaaagaa actgtataga gcaaaatctc
    10741 caccctcagg tttgaaagcg gagattgttt ttattttttc aaggtttgta gtcgtggaac
    10801 aatggctagg gttagaattg cagaaatgac caattcagca attgaatttg ttgagataac
    10861 ggttgccaga agaagttgga tatttccgtt ataaacattt ccaaatagga agaagattcc
    10921 tccaaggaca aagatagtat ttgtcaagga accaagagcc ccagctaaaa tcagaccagt
    10981 cttgtttttc atcagtttat agactaagta aggagttaaa ccaatcaaaa tacgtgggac
    11041 aatggcaatg atagctgagt agatgtttcc gtttggtacg aagggagaga agaggtagct
    11101 tgtcggtaga atcgtaatcg tgttaaccgt caagctaagt aatcccatca aaaatccaag
    11161 tgtaacccca actcgtggac cataaataat gctggcaata atgacaggaa tatgaacaat
    11221 ggtcggtttg attggaaatg gaaaaaggtt aaagataagt gagctcagaa agtgtatcac
    11281 gagcatggta gcaaaaaaga tagcaatggg tgcaatatta gagcgttttt tcatcgatag
    11341 tttcctttat tctttctaaa ataattgtga ggtcagctaa agctcctcgt ccgtggtctc
    11401 cacaagctag tagggattcc ttaggagcaa tcagctgata gccgtaggtt tctaatgttt
    11461 tcagattatt ctgagttact ggatggtcat acatttttgt attcatagca ggagctatta
    11521 gtttgggaat atgacttggt agggctagag ctgtactggt taccatgttg tccgcaaatc
    11581 cgtgagctag ttttgcaata gtgttagcag ttgcaggtac cacgataaat aaatctgctt
    11641 tttttccaag ttcgatatga ttgacctgat caggataggg ttctttcatg acatccaagt
    11701 ggacaggatt ctgtgagagt acctgtagtg tcaaaggttg gataaactct gtagcagcct
    11761 gagtcattaa gacagtgact tgatggcctt gtttttttag agaactgact aaatctgccg
    11821 acttataaga ggcgattgaa cccgttacag ccaagagaat gtttgccata gttttccttt
    11881 ctaagaatga taggcttgaa ttttttcaag gaggagttct gcaatttctt ctttagtctg
    11941 gactgtttga agctgatttt tctcaacaaa tatagctcgg tgctgatctg ctgaaatttg
    12001 agtcaggtca ttcgcgatga ttaaatctgc ttgattcttg ataagacttt ttcgtgcaat
    12061 gtcaaccaga tgatcttcgg taacatcaac cagcagtttg aaaccaatca gatgaatagt
    12121 aggattccat tccttgacta gggatatgat tttgggtgtc tttttaagga acaaaacctg
    12181 aacctcatca gttgaagaaa tcttggtctg atgattctgc ttgcttaaaa attcttttag
    12241 attggagcta gcctgaactt cctcaagccc tgtcatataa acaggagtgt agtcagaaac
    12301 agccattgag tggatcaaga cctgataatc ctgaacacgt tcttgcattt ctattagaag
    12361 gtccttggta ttggtaattt ctcgaatact taggttagga tgaggctctg gcttcagagc
    12421 tcgttttgtc gtaattaaac aaacttcatg ccctgcagaa agcaaagtct ctgtgataat
    12481 tttccccaag tgacctgtag aatggttagt gatagagcgg acgctatcga tagcttcact
    12541 ggtaccgccc gatgtaacta aaattttcat agcactattg tacacaaaaa taagcggtaa
    12601 gtaaaatgaa agtgataaat ttttggtaaa aacgaagatt tattatagtt tcaaactata
    12661 aagccatata aaatttaaga aaggactcta aaagtcttaa aaacagagat atttacgaac
    12721 gtttagagag ttttaagatg ttaaaaatct tgttttgtga tataatgaat tatcatataa
    12781 gaggttagag gaattttgaa tgaaaacaga tattgaaatc gcacagagta ttgagttgaa
    12841 gccaattgtt gatgttgtag agaaacttgg tatttcttac gacgatttgg agttgtacgg
    12901 aaagtacaag gctaaactca gctttgataa aattcgggca gttgagagca atccagtcgg
    12961 taaattgatc ttggttactg ccatcaaccc aacacctgca ggtgaaggaa agtcgacgct
    13021 taccattggt cttgcggatg ccttgaacaa gattggcaag aaaaccatga ttgctatccg
    13081 cgaaccgtct cttggtccag tcatggggat caagggtggt gctgctggtg gtgggtatgc
    13141 acaagttctg ccaatggaag acatcaacct ccactttact ggagatatgc atgctattac
    13201 aactgccaac aatgcccttt ctgccttgat tgacaaccac ttgcaccaag ggaatgagct
    13261 gggaattgat caacgtcgta tcctctggaa acgcgttgtg gacttgaacg accgtgcgct
    13321 ccgccatgtg actgttggtc ttggtggtcc tctaaacggt attccacgtg aggatggttt
    13381 tgatattaca gttgcttcag aaatcatggc aattctttgc ttggcaacgg acatcgagga
    13441 cttgaaacgt cgtttggcga atatcgttat tggttatcgc tatgaccgta cgcctgtttc
    13501 tgtaggtgat ttgcaggttg agggtgcctt ggctttgatt ttgaaggatg ctattaagcc
    13561 aaacttggtt cagacaattt acggtacacc tgcctttgta cacggtggtc catttgccaa
    13621 tatcgctcat ggctgtaact ctgttttggc gacgacaaca gcccttcact tggctgatta
    13681 cactgttact gaagctggtt ttggtgcgga ccttggtgct gagaaattcc ttgatatcaa
    13741 gacaccaaac ttgccaacat ctccagatgc agttgttatt gtcgcaaccc ttcgtgccct
    13801 taagatgaat ggtggtgtgg ctaaagacgc tctgactgaa gaaaatgtag aggcagttcg
    13861 tgcaggtttt gctaacttga aacgccacgt tgaaaatatc cgtaagttcg gtattccagc
    13921 agttgtagct attaacgaat ttgtatctga tacagaagct gaaattgcag tcttaaaaga
    13981 actctgtgcc tcaatcgatg taccagttga attggctagt gtctgggctg atggagcaga
    14041 aggtggagta gcacttgccg aaacagttgt taagacaatt gctgaaaatc cagctaacta
    14101 taaacgtttg tatgataatg acctttctgt ccaagaaaag attgaaaaaa ttgtcactga
    14161 aatctatcgt ggtagcaaag tgaactttga gaagaaatct caaacacaaa ttgctcaaat
    14221 cgttcaaaac ggttgggaca aattgccaat ctgtatggct aaaactcaat acagtttctc
    14281 agacaatcca aatgcgcttg gagcaccaga aaactttgaa attaccattc gtgaattggt
    14341 accaaaatta ggtgcaggct tcatcgttgc cttaactggt gatgttatga ccatgccagg
    14401 tcttccaaaa cgtccagcag ctctcaacat ggatgttgaa agcgatggaa ctgtactagg
    14461 cttgttctag tggattgaaa ttgactttaa gtgagtttgg aaataatatc caagctcatt
    14521 ttttctcaga ctgcaagcag ttcatcaata agatttatac tcttcgaaaa tctcttcaaa
    14581 ctacgtcagc ttcaccttgc cgtaggtata tgatactgac tctgtcagtt ctatccacaa
    14641 cctcaaaaca gtgttttgag caacctgcgg ctagcttcct agtttgctct ttgattttca
    14701 ttgagtataa gtagatagaa aagttatata ctatctattg aaaatattat aaatcagata
    14761 caaagacagg tgaaaccttc tgtgttatac tagagatatg ttagatttga aagaatacgg
    14821 tatcgtcatg tggccggagg agaaagtcat ttctttccgt gagaaacttc tcgcttggta
    14881 tgatgaaaac aaaagagatt tgccttggag gagaagtaaa aatccttatc acatctgggt
    14941 atctgaaatc atgcttcagc agaccagggt ggatacagtt atcccttact acgaaagatt
    15001 cctggactgg tttccaactg tcgaaagtct ggcaactgcg cctgaggaga gtttactgaa
    15061 agcttgggag ggcttgggct attattctcg agttcgcaat atgcaggctg cagcccagca
    15121 gattatgact gactttggtg gccaatttcc aaatacctat gaaggaattt ccagcttgaa
    15181 agggattgga ccttacacag caggagccat ttccagtatt gcttttaact tgcctgagcc
    15241 agctgtagat ggtaatgtca tgcgggtctt ggcgcgtctg tttgaagtca accacgatat
    15301 tgggattcca agtaatcgaa aaatttttca ggcaatgatg gagatcttga ttaatccgga
    15361 tcgtccgggt gattttaatc aagccttgat ggacttaggg tctgatattg agtctcctgt
    15421 aaatcccaga cctgaagaaa gcccagtcaa ggactttagt gcggcatatc agaatggaac
    15481 catggaccgt tatccaatta aatctcctaa gaaaaagccg gttcctattt atcttaaagc
    15541 cttagttgtg aaaaattctc aaggacaatt tttacttgaa aaaaatgaaa gcgaaaagct
    15601 cttggcaggt ttttggcatt tcccctttat agaagttgat aacttttcgc aagaagagca
    15661 gtttgacctc tttcatcagg ttgcggaaga aagtgtgaac tttggcccca gtccagaaga
    15721 gagtttccag caggactatg acctagatgt tgattggctt gatgtttgtt ttgatacagt
    15781 ccagcatgtt tttagtcatc gaaaatggca tgtacagatt gtagcaggtc aggtgagtga
    15841 cttccatgat ttttcagata gggaagttcg ttggctttca ccagaagaat tcaagaatta
    15901 cccacttgcc aaaccccaac aaaaaatctg gcaggcttat gcacaagcca acttagacag
    15961 tagccaagac tagctattgt gtcttctgac tattttttgg tataatagta gagaaaaagg
    16021 tgaacatatg aaaaaaatac taattgtaga tgatgagaaa ccaatctcgg atattatcaa
    16081 gtttaatatg accaaggaag gttatgaagt tgtaactgct tttaatggtc gtgaagcgct
    16141 agagcaattt gaagcagagc aaccagatat tattattctg gatttgatgc ttccagaaat
    16201 tgatggttta gaagttgcta agaccattcg taagacaagc agtgtgccca ttcttatgct
    16261 ttcagccaaa gatagtgaat ttgataaggt tatcggtttg gaacttgggg cagatgacta
    16321 tgtaacgaaa cccttctcca atcgtgagtt gcaggcgcgt gttaaagctc ttctgcgtcg
    16381 ttctcaacct atgccagtag atggtcagga agcagatagt aaacctcaac ctatccaaat
    16441 tggggattta gaaattgttc cagacgccta cgtggctaaa aaatatggcg aagaactaga
    16501 cttaacccat cgtgaatttg agcttttgta tcatttagca tcgcatacag gtcaagtcat
    16561 cacgcgcgaa cacttgcttg agactgtctg gggttatgac tattttggtg atgtccgcac
    16621 agttgatgtg actgtacgac gtctgcgtga gaagattgaa gatacgccca gccgaccaga
    16681 gtatatcttg acgcgccgtg gtgtagggta ttacatgaga aataatgctt gatttactga
    16741 aacaaaccat ttttaccaga gattttatct ttatcctgat tttgttaggt ttcatccttg
    16801 ttgtgaccct cttattactg gaaaatagac gtgataatat tcagttgaag caaatcaatc
    16861 aaaaggttaa agatttgatt gcaggagatt attccaaggt tcttgatatg caaggtgggt
    16921 ctgaaatcac caatattacc aataatttga atgacttgtc ggaggttatt cgtctcactc
    16981 aggaaaatct agaacaagag agtaagaggc taaatagtat tctgttttat atgacagatg
    17041 gggttcttgc gactaaccgt cggggtcaga ttatcatgat taacgataca gccaagaagc
    17101 aactggggtt ggttaaggaa gatgttctga atagaagcat tttggaattg ctcaagatag
    17161 aagaaaacta tgaattgcgt gatttgatta cccaaagtcc agaattgttg ctagattccc
    17221 aagatatcaa tggcgaatat ttgaaccttc gagttcgctt tgccttgata cgtcgagagt
    17281 ctggctttat ttcaggtttg gtggctgttt tgcatgatac gacggagcag gagaaggaag
    17341 aacgcgaacg aagactcttt gtttccaatg ttagccatga gttacggact cctctgacta
    17401 gcgtaaaatc ctatcttgaa gccttggatg agggggcttt gtgtgaaact gtagcaccag
    17461 actttatcaa ggtttctctt gatgaaacca accgtatgat gcgcatggtg acggatctcc
    17521 tccatctttc acgtattgat aatgctacca gtcatctaga tgtggaactg attaacttca
    17581 ctgcttttat tacctttatc ctcaatcgtt ttgacaagat gaaaggacag gaaaaggaaa
    17641 aaaaatatga gttggtgaga gattatccca tcaattctat ctggatggaa attgatacag
    17701 ataagatgac gcaggttgtc gacaatattt taaataatgc tattaagtat tcgccagatg
    17761 ggggtaaaat cactgtcaga atgaagacaa ctgaagacca gatgatttta tccatttctg
    17821 accacggttt ggggattcct aagcaggatt taccacgtat ctttgaccgt ttctatcgtg
    17881 tggatcgtgc tagaagtcgt gcacaaggtg gtacaggtct aggactgtct atcgctaaag
    17941 aaattatcaa acaacataag ggctttattt gggccaagag tgaatacggc aagggttcaa
    18001 cctttaccat tgtgctccct tatgataagg atgcagtgaa agaagaagta tgggaggatg
    18061 aagtagaaga ctagaatgag tgaaataggc tttaaataca gtattttagc gtcgggttcc
    18121 agtggaaatt ctttttatct ggaaacctca aaaaagaagc ttttagtaga tgcaggcttg
    18181 tctggcaaga aaattaccag tctgctagct gaaattaacc gtaagccaga agacctggat
    18241 gccatcttga ttacccatga gcattcagat catatccatg gagtaggcgt tttggctcgc
    18301 aagtatggta tggatcttta tgccaatgaa aagacctggc aagctatgga aaatagtaaa
    18361 tatcttggca aggtggattc ttcgcaaaag catatttttg aaatgggtaa aacaaaaact
    18421 tttggagata tcgacatcga gagttttggt gtaagccatg atgcagtcgc accgcagttt
    18481 tatcgcttta tgaaggatga caagagtttt gtccttttga cagatacagg ttatgtcagt
    18541 gaccgtatgg cgggcattgt cgaaaatgcg gatggctatc ttatcgaggc caaccatgat
    18601 gtagagattt tgcgatcagg ttcttacgct tggcgactca aacaacgaat cctatctgac
    18661 cttggtcacc tttctaacga ggacggtgct gaagctatga ttcggacgct aggaaatcgt
    18721 actaagaaga tttaccttgg gcatttatct aaggaaaaca atatcaagga actggctcat
    18781 atgaccatgg tcaatcagct ggctcaagct gatctgggag tcggagtaga ctttaaggtt
    18841 tatgatacct caccagatac cgcaacacca ttgacagaga tatagaaaga acgctgagaa
    18901 ggtgttcttt ttatattgac tgaacaccta aaaagtaata caatggtgtt accattaaaa
    18961 aagggagcac aaaagatgac tactattaca ttaaaagttt ctgaagctga taaaacattt
    19021 atgaaagcaa tggctaagtt tgaaggagtt tccctttcgg aacttattcg caccaaaact
    19081 cttgaagccc tagaagatga atacgatgct cgtgtggcag atttagccta tcaagagtat
    19141 ttagaagact tggaaaaagg agttgaaccc attacttggg aagaaatgat gcatgattta
    19201 ggcttgaagg atgaataatt tgtataaatt agttccaaca agacgtttta tcaagcaatt
    19261 gaaaaaattg gaccgttata cgcagaagct aattacaaac tatttacaaa ccaatgtttt
    19321 ggaagatcca agacgacacg gaaaggcttt ggttggtaat cgcgttggtc aatggcgcta
    19381 tagaattggt aattatcgag ttatcgtaca aattgtagat gatgaattag ttgttgctac
    19441 tctagaagtt ggtcatcgga gagatattta ttgaattact tttttcaagt aaattttca
//
LOCUS       DAVKHZ010000037        19415 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000037 DAVKHZ010000000
VERSION     DAVKHZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.037"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            156..1637
                     /locus_tag="VWW99_001197"
     CDS             156..1637
                     /locus_tag="VWW99_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9539043.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1999..2994
                     /locus_tag="VWW99_001198"
     CDS             1999..2994
                     /locus_tag="VWW99_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9539044.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIAIPTYFWGDKTTKKI"
     gene            3121..4431
                     /locus_tag="VWW99_001199"
     CDS             3121..4431
                     /locus_tag="VWW99_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9539045.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            4527..5429
                     /locus_tag="VWW99_001200"
     CDS             4527..5429
                     /locus_tag="VWW99_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9539046.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYTFTDYYLLTPDERKFVGFENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            5431..6276
                     /locus_tag="VWW99_001201"
     CDS             5431..6276
                     /locus_tag="VWW99_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9539047.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFLGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRFAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            6286..7605
                     /locus_tag="VWW99_001202"
     CDS             6286..7605
                     /locus_tag="VWW99_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9539048.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWIMYTGHIEEKTGVHQVQNLAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHMDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(7813..7885)
                     /locus_tag="VWW99_001203"
     tRNA            complement(7813..7885)
                     /locus_tag="VWW99_001203"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7850..7852),aa:Lys,seq:ctt)
     gene            complement(8150..9421)
                     /locus_tag="VWW99_001204"
     CDS             complement(8150..9421)
                     /locus_tag="VWW99_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9539049.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            9691..9930
                     /locus_tag="VWW99_001205"
     CDS             9691..9930
                     /locus_tag="VWW99_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539050.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10095..10274
                     /locus_tag="VWW99_001206"
     CDS             10095..10274
                     /locus_tag="VWW99_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539051.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10411..10590
                     /locus_tag="VWW99_001207"
     CDS             10411..10590
                     /locus_tag="VWW99_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9539052.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            10627..11079
                     /locus_tag="VWW99_001208"
     CDS             10627..11079
                     /locus_tag="VWW99_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9539053.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11370..11840
                     /locus_tag="VWW99_001209"
     CDS             11370..11840
                     /locus_tag="VWW99_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9539054.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            11859..12947
                     /locus_tag="VWW99_001210"
     CDS             11859..12947
                     /locus_tag="VWW99_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9539055.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENSKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            12928..13356
                     /locus_tag="VWW99_001211"
     CDS             12928..13356
                     /locus_tag="VWW99_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9539056.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECAAREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSLFMVGQLRQEQFDN
                     IIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            13353..13826
                     /locus_tag="VWW99_001212"
     CDS             13353..13826
                     /locus_tag="VWW99_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539057.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            13961..14914
                     /locus_tag="VWW99_001213"
     CDS             13961..14914
                     /locus_tag="VWW99_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9539058.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNT
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            14916..15659
                     /locus_tag="VWW99_001214"
     CDS             14916..15659
                     /locus_tag="VWW99_001214"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9539059.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDG
                     IKRLARVLDVEARQFELVEELDDNVELPVQVTISSGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15670..17466
                     /gene="pepF"
                     /locus_tag="VWW99_001215"
     CDS             15670..17466
                     /gene="pepF"
                     /locus_tag="VWW99_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9539060.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALINLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            17713..18504
                     /locus_tag="VWW99_001216"
     CDS             17713..18504
                     /locus_tag="VWW99_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9539061.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIVEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLSADANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
     gene            18615..19283
                     /locus_tag="VWW99_001217"
     CDS             18615..19283
                     /locus_tag="VWW99_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9539062.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         5984 a         3233 c         4244 g         5954 t
ORIGIN      
        1 ggtaaaataa gaaaattgtt tatatattgt ctccgtagtg ttattatacg aaataaaaga
       61 ttttaggaaa agtcgttata tcatgctata cctattcttt gtggtataat tgcaagaggt
      121 ttaatccgat aatttataaa gagagaaaga tattcatgag agatttatta tctaaaaaaa
      181 gtcataggca attagaatta ttagaattat tatttgaaca taaacgttgg tttcatcgtt
      241 ctgaactagc agagttatta aattgtacag aacgtgcagt caaagatgat ctatcccatg
      301 ttaaatctgc ttttcctgac ttgatttttc attcttctac taatggtata cgcattatta
      361 ataccgatga tagtgatatt gaaatggttt accatcattt ctttaaacat tcaactcatt
      421 tttcgatttt agaattcatc ttctttaatg aaggttgtca agctgagagt atttgtaaag
      481 aattttatat cagttcatct tcgctctatc gtattattag ccaaatcaat aaagtgatta
      541 aaaggcaatt tcaatttgaa gtcagtctga cccctgttca aatcattgga aatgagagag
      601 acattcgtta cttttttgca caatattttt cagaaaaata ttatttccta gaatggccat
      661 ttgaaaattt ttcatcagag ccactatctc aattgttaga attggtttat aaggaaacaa
      721 gctttccaat gaatttgtca acgcatagaa tgctaaagtt gctcctagtt acgaacctat
      781 atagaataaa gtttggtcat tttatggaag tagataaaga ttcttttaac gaccaaagtt
      841 tggatttttt aatgcaggca gaaggaatag aaggtgttgc tcagagtttt gaatcagaat
      901 acaatatctc tttagatgaa gaagttgttt gccaattatt tgtgtcttac tttcaaaaaa
      961 tgtttttcat agatgaaagt ctctttatga aatgcgtaaa aaaggatagt tatgttgaaa
     1021 aatcttacca tctattgagt gattttattg atcagatttc agtcaagtat cagattgaaa
     1081 tggagaataa ggataatctg atttggcatc tgcataatac cgcacatctg tatcgtcaag
     1141 agttgtttac tgagtttatt ttatttgatc aaaaagggaa tacaatcagg aactttcaaa
     1201 atatttttcc aaaatttgtt tcagatataa aaaaagagct ttctcactat ttagagactc
     1261 ttgaggtttg ttctagttca atgatggtaa atcatctatc ttataccttc atcactcata
     1321 ccaaacattt ggtgattaat ttgctacaaa atcagcctaa actaaaggtt ttagttatga
     1381 gtaattttga tcagtatcat gcaaaattcg ttgcagagac actttcttat tactgtagca
     1441 ataattttga actggaagtt tggactgaat tagaattatc aaaggaatct ttagaagatt
     1501 catcttatga tatcattatt tctaatttta ttattcctcc tattgaaaat aagagactca
     1561 tctattcaaa taatataaac acggtctcac tcatatattt gttaaatgcc atgatgttta
     1621 ttcgattaga tgagtaacca aaaataacaa aaaaacgcta tttcgcgttt gaactgtacc
     1681 tcaaaagtta gacagaaaaa gtctaatttt tggggtgttt ttgttaagaa aataccttca
     1741 atcatttaag caaaatgtac atctagattt atagccatct aaaactcttg ctttttttgt
     1801 agaagatacg aaaaaagcca tgaaaccaag gcttttcctg ttttatgtag atgtttacac
     1861 atgaaaattg aagtattttc aaaagctaat tggagtgatt ttattcatga tgggataaat
     1921 gatctggcca aaaatgattg agaaatagtt gcttaaaaag catttgcatg atactatata
     1981 ttgaaaggag gtgataatat ggtagaacaa agaaaatcaa ttaccatgaa agatgttgct
     2041 ttagaagcag gagttagtgt tggaactgtt tcacgtgtaa ttaataaaga aaaaggcatt
     2101 aaagaagtaa ctttaaggaa agttcaaagg gctataaaaa cattggacta tatccctgat
     2161 aattatgctc gaggtatgaa aaagaataga acagaaacta tcgctttgat tattccgagc
     2221 atatggcatc catttttttc ggagtttgcc tatcatgttg agaaagagtt attaaaatat
     2281 aattataaat tatttatttg taacgctgat agcgattcac aaaatgaaat cgaatatatc
     2341 gaaatgttga aacaaaataa agtggatgga attattggta ttacatattc tgatattgac
     2401 aaatatattt tatctgattt accgtttgta agtatagata gacatttttc ggaaaatgta
     2461 tgctatgtta cttcagcaaa ttacaaagga ggacaaactg cagcagagga actggttaga
     2521 agggataata agcaccttgc ttttattggt tcaattaata tgtatgagaa tgagactata
     2581 ctcagaagta aaggattcag agaaaaagct aaggaattag gagtagatgt ctataatttt
     2641 gaaatgaaag aacctttaat aaatcctgaa gatcagataa gaattttttt gcaatctcat
     2701 aaagaaattg atggtatatt tacaataaat gatatgatgg ctctgagagt tattagtgtt
     2761 atgaaagaac tggggaaaat agcacctgat gattaccaga taattgggtt tgatggttta
     2821 aaaacttctg tcgatcaatc ttatctagtg tctacaatag ttcaagatat tggtatgatg
     2881 gcaaaagaat ctgttgcaat gatactgaaa atgattaagc gagaagagat tctagaaaaa
     2941 cgaatcgcaa ttcccacata tttttgggga gataaaacaa caaaaaaaat ttaaaaagcg
     3001 cttgacaatt aatgaaagcg ctgttataat ataatcaaca aatgaaacgt ttcaaaaata
     3061 ggctagaagc ttgtttaaaa aataaataaa aaatgaaacg tttcaaaaag gaggaaaatg
     3121 atgcgtaaag gtacaatgtt atctgttgtt gcggggctat cgttagctat acttgcaggt
     3181 tgtagtggag gaactaactc aaaacaagcg agttcttcaa atgatattaa agtctgggta
     3241 caattctctg acgaaacaaa tgaaggaaaa gcttggcaaa aagttgttga taactttaat
     3301 caatcaggaa aaagtaagta taaggttgta actgagtata ttcctcgtag tggtagtggt
     3361 ggaggatatg aagacaaggt taatgctgct attacgacta atagtcttcc agatgttatt
     3421 acccttgatg gaccaaatac ggcagcttat gcaaaatcta aagtaattac tccgctagat
     3481 gattatctaa aagataataa tatggatgat gttttggaca gtattaaaca acagggaact
     3541 tataatggga aattctatgc atttggattt tctgaatcga atgttggtgt ttattacaat
     3601 aaaaaaatgt tcaaagaggc cggaattgcg gaatcagagc ttccaacgct agaaaagcca
     3661 tggacatggg acgaatttaa tacgattgct aaaaaattga aagatcatta taacaaacca
     3721 gcaattgatt ttcgtattaa ttcaaacgat gaaatgttgc catatgctta tatgccgttg
     3781 atttggtcaa ataatggttc tgtcgtaaac gaagatggaa caaaggcaga aggatacttc
     3841 aactccaaag agagtgtaga agcagttcaa ttcattcaaa atcttgtaaa agaaggctat
     3901 acaactgtta gtccagtcga aaaaggtttt gaaacaggag agtatccaat gcttttgagt
     3961 ggttcttgga cgattgctga catgaataca aactacaaag atattgattt tggtatcctt
     4021 ccttacccag tatctagtaa gacgaaaaaa ttagtatccc cttcaggtag ttggcaatta
     4081 gctgtgacga caaaatctga taaaaaagag gccgcatctg aatttgttaa atttgctaca
     4141 aatacagaat caagtgaaat tattagcttg gggaattcgg tccttccaat tcgtaaatct
     4201 acaattgaaa atatcaaaga taaagtatcc gaaccaatgc gtttcttgat ggaacaaaat
     4261 tcaaaaacag ctcatgctcg cccagttgtc gtagcatatc ctcaggtttc tcgagctttt
     4321 caacaagcaa tgcaagatat tagctattat gaagaaaatc caaatgtgca aaaagtcttg
     4381 gatactcgta caaaagaaat gcaaactgct attgatcagt cactcaaata atctgtaaaa
     4441 aagaaggtgg aaggaaaatg tttggaacta ccttcccctt tcttgttaat agcctagttt
     4501 gatatatatg attaaaggag atggatatga gtaaagaaac tgttgtggat acaaaaagac
     4561 gtgaaaaaat aaaagataac atactaggtt atagtttttt agctccagct ttaattttat
     4621 taggtatctt tttggtaatt ccagttggaa tggtcattta ttacactttt acagattatt
     4681 atttgttgac accagatgaa agaaaatttg ttggatttga gaattttatt cgattaacaa
     4741 aagatccgat tttcttaaag agcttcttga atactttgaa atttgttgtt tggattattc
     4801 cagttcaatt gggagcggcc ttaggaatgg ctttgattgt caataaaaag cgaaaaggaa
     4861 atatgttttt taaagtggct ttttttgctc cagttgtcat gtccttggta gttatttcta
     4921 ttctttggct ttatctttta aatccaaata gtggcttatt aaatgctatt ttaaataagg
     4981 taggtattgc ttctcaacct ttcttgacta gtcctaaaca agctatgtat gcaattgttt
     5041 ttgtttctgc ttggcagggt gcaggttatc agatgctttt atttttagga ggaatgcaga
     5101 atattccaca agatgtctat gaagctgctg aattagatgg attctcaaaa tgggctcagt
     5161 ttcgttatat cacgatgccc ctactaaaac caacagccct gtttgtattg ttaacaactt
     5221 taatttctgc ttttaagctg attgtacaac caatggttat gacgcaaggt gggccattaa
     5281 actcaacaat tactatggtg tactatattt atcaacaagg ttttactgat cgtcttgtag
     5341 gatattctag ctcaatcgct ttagtattta caacgttaat tggtatgatt agcttagtac
     5401 aacgtcgcgt tctgaaggag gatgactaag atgaaacgat ttaaaccaac aacaatctta
     5461 gaatatattc tgttaacttt attggcagga ctcttccttt ttcctatggt ttggatggta
     5521 gtttcagcta tgaagccaga agcggatgtt tataaaaatt tgactagttg gcaagccttc
     5581 ttaccaagtt taaatcccgc tgattggttt aaaccttatc aggaagtatt tgagcgcttt
     5641 agtattttta catatctagg gaacagcatt ttttatgctg gaacgtttgc tattggttct
     5701 attattgtca atgcactggc cggttttgct tttgcaaaag tcaatttttt aggtaagaaa
     5761 attttatttg gtttcttact tgctttactt attattccag tggaaactgt gttaattcca
     5821 caatttacca ttatcaattc cttaggtttg gtcaataatc gttttgcagt tattattcca
     5881 ggattggcga gtgtatttaa tatttactta tttaggaatt tctttattgc aattccagaa
     5941 gagattcttg agtctgcaaa gatggacgga gcaagtatcg ttagaatttt ctttagaatt
     6001 atgcttccaa tgtctaaacc tgcagttgct acagttggtg ttttatcatt tatttcaagc
     6061 tggaatgatt atatctggcc attgatggtt ttgacagatt cttctaaatt ttcaatgcag
     6121 gttgcaatta caactattaa tacaactcaa ccagtttata tcaatcaggt aatggctgta
     6181 ttaactattt caacgattcc gttgattttg gtctatgtag tcgctcagaa atatattgtt
     6241 caaggtctgg gtggttccgg aactggaata aaataggata tacttatgat aaataaaaca
     6301 tttacagtag aaaaagccaa tcgttttata gcagaaaata aacatctcgt taatactcaa
     6361 tataagcctg aagaacattt ttcagctgag attggttgga tcaatgatcc aaatggattt
     6421 gtttattttc gtggagaata ccatctcttt tatcaattct atccatatga tagtgtttgg
     6481 ggacctatgc actggggaca tgctaaaagt aaggacttgg tgacttggga gcacttgcca
     6541 gtggcacttg ctcctgacca agattatgac cgaaatggtt gtttctcagg ctctgccatt
     6601 gtcaaggatg atcgcctctg gatcatgtac actgggcata tcgaagaaaa aaccggtgtc
     6661 caccaagtgc aaaatctggc attttcagat gacgggattc actttgaaaa gatttcccaa
     6721 aatccagttg taactggaac agacttgcca gatgagttga ttgctgctga tttccgtgat
     6781 ccaaaactct ttgaaaaaga tggacgctat tactccgtag tagctgccaa acacatggat
     6841 aatgtgggct gtatcgttct actagggtcc gataacctag tagaatggca gttcgaatcc
     6901 atctttttaa aagggggaga acaccaaggc tttatgtggg aatgcccaga ttacttcgag
     6961 ttagatggga aagattgcct tattatgtca cccatgcgtt atcagcgtga gggagactca
     7021 tatcataaca tcaactcatc gcttttgttc acgggtaagg tagattggag agaaaaacgt
     7081 tttatcccag aatcagttca agaaattgat catggccaag acttctatgc gcctcaaaca
     7141 ttgttggacg atcaaaatcg tcgtatcctg attgcttgga tgcagacatg ggggcgtacc
     7201 cttccaaccc atgaccaaga acacaaatgg gcatgtgcca tgactttacc tagaattcta
     7261 agattggaag atggcaaact aagacaattc cctgttaaaa aaggccaata tcaaatccaa
     7321 atagataaag attgtcatta ccacttagga aatgatacag attatcttga atttggttat
     7381 gacagtaatg cgcagcaagt ttacattgat cgtagccatc ttattcaaaa aattctaggt
     7441 gaagaagaac aggacactag tcgacggtat gtagatattg aagctaaaga attggaagtt
     7501 gttctagata aaaattccat cgagattttt gtcaatcaag gtgaagcaag cttgactgca
     7561 acttattact taacggtgcc agctgagcta tcacgaattg attaaaaatt aagttatttc
     7621 tcctaaagaa aaagttctct ttctaaaata gtggaaagag gactttttgt gttttgggta
     7681 tataagctta gtttatggta tttgtaaaat tggtgttgga ttatgattta agctagtttt
     7741 ctaaagaatt tgaaaaaaat tttatttaag caaaaaaacc ttggttccaa ggcttttcct
     7801 gttgtattta tatgccccct acagggatcg aacctgtgac ccacggatta agagtccgct
     7861 gctctgccag ctgagctaag gaggcaaaga aaaaagctgt attggtgccg aaacttcacg
     7921 gtttgtattg aacccgcgca attaagcagg tgggcaactc gctctaactg aagctgtttc
     7981 cgtgtgagac ggcctacatg ctgttagaag acttttgttt ccctaataat acaaaaaata
     8041 gtcggtcaac acttaagtgt gaagtcgtac accacagcgt ttctatattt atatgatacc
     8101 acttttttaa aaaaaatcaa gaggaaagtg caattttttg aaaaggattt cagatttttc
     8161 gcaaatggtc gatagcttcc tttctttgag ccaaagcccg ttcatattta ccagtatctt
     8221 ctgcttggaa atagtgatga ttacgaattt tttctggcag atagtcttgc tttacccaat
     8281 ttccaggata gttgtgtgga tagagatagt cttgggcatt ccccagttcc ttgcttccac
     8341 tgtagtgccc atcacgtagg tgtcgcggaa taggcaagtg ccctgatgtt ttgaggtcag
     8401 caagtgcctt atccatagct acataggctg agttggattt tggagaaagg gccaaatcaa
     8461 tcacgacatt ggcaatgaga atgcgggctt ctgggaaccc aatcttctgg gcagcatcca
     8521 gagcagtcac ggtatgaatc tgggcctcag gattggctaa gccgatatct tcataggcga
     8581 taacagtcaa gcgacgagcg agactaggca gatccccagc ctcaatcaag cgggcagcat
     8641 agtggagact ggcatccaca tctgagccac ggatagactt ttgcagggca gatagaacat
     8701 catagtgacc gtctccatcc ttgtccatag tgatgtagct tctctgaaga ctattttcca
     8761 tgatgtctaa ggtgatgtgg cgaatgccct cgtcattctc aggggtagag agaacagcca
     8821 agtccagtga gttaaaggca gagcgaaggt ctccgtttgt agaggttgcg atgaaatcca
     8881 gcgcatcctc atctagttct attggaaaat caaaaccacg ttcagggtta cttagagcta
     8941 tctgcagggc ctctttgacg tcttggttag acagaggttc caactcgaaa atttgaacac
     9001 ggctacgaat ggcaggagtg acagagaaga aaggattttc agtcgttgct ccaatcatga
     9061 tgaccagtcc actttccaag agaggcaaga ggaagtcttg cttggtctta tctagtcgat
     9121 gaatttcgtc tagcaatagg acgagaccac cagaaaattt agcttcttcc gagatttctt
     9181 gcagtcgctt tttactatca actgtcgcat tgaaagttcg aaaggcatac ttggtcgttc
     9241 cagcgatggc agaggcaata ctggttttgc cgattcccgg agggccatat agaatcatgg
     9301 aggacaggcg gttggcttcc accatgcggc ggatgatttt tccaggtccg accagatgct
     9361 cctgaccgat gacctggtcg atggttttag ggcgcatgcg aagcgcgaga ttgtctggca
     9421 tagcagtcct ttctaacgtg gatttgagta gaatgactca tctactatat cttactatgt
     9481 ccattctagc ctattagttg tgatatttca atggttttgt aataatcagg gagtgagata
     9541 gaatcttttt tactatttaa tttaatgagg agaggtatca gcatgagaga tttgattttc
     9601 cttaaagctc aaaataggat agttgagcct aaagaagtgg aagtgctgac ttgagaaata
     9661 atattgctga atgtcattgt atgaactata atgaaaagag aggtgataag caatggaaat
     9721 gatggaccaa gtcaggaaat tttgattttt acaaaacaaa ttcgtcattg gattcttagt
     9781 gatcaagtta tttcagggaa aagaaaactt ttcttccggg aagatactcc aaaagagatt
     9841 ttagatttgt acgaaaacat taaatctaaa cttgattttg cttaccaaga agttcattct
     9901 aataatgggt tgaaaaaata tgaaaaatag aataattgat gtttttgaag tagtaaatcg
     9961 cctccttgta ataactgttg aaaatcctga tttcgaagac ttgagagtta atcatactca
    10021 atgaaaatca aagagcaaac taggaaacta gccgcaggct gtacttgagt acggtaaggc
    10081 gacgttgacg tggtttgaat ttgattttcg aagagtatca gtttgtaaaa attggagata
    10141 aaaaatatag agtacgcagt gttcctatga ttcattcaac tccgcctcaa tctgtcttag
    10201 atcgagatac ttttacaatt gactatacag atgatgaatt gctcgataaa gaagcagttt
    10261 ttactactca ttagaaaaat aggtctgaat aaattggctt cccttagaac cacatctagg
    10321 ggcttttatg tataatagaa taataagtat cactccttta caatacgtgt aacacgtgtt
    10381 ataatggtgt tgtcaggagg taaaagcgct atgcctatga cacaaaaaga gatggttaaa
    10441 ctccttacgg cccatggttg gataaagact agaggcggta aaggctccca tattaaaatg
    10501 gagaagcaag gggaaagact tatcacaatc cctcatggtg agctgaataa gtacactgaa
    10561 agagggataa gaaagcaagc tgggttgtaa aattcagccc ctgttttttg atatggaggt
    10621 agtgaaatgt tagttacgta tccagcttta ttttattatg acgacacaga cggaacagaa
    10681 gcgacttatt ttgtccattt cccagatttt gaatactcag ctacacaagg agaggggatt
    10741 tctgaggctt tggctatggg gtcggagtgg ctagggataa ctgttgcaga tttgatagag
    10801 agtgatggag agttacctca gccatcagat atcaatagct tgtctttaat tgataatgat
    10861 ccttttaaag atgatgaaga tttcgtgtca acctatgacc ttgataaatc ttttatttct
    10921 atggtatctg ttgatgtatc agaataccta ggaagtcagg aacccataaa aaagactttg
    10981 accataccaa aatgggcaga taagttggga cgagaaatgg gacttaactt ttctcagact
    11041 ttgacagacg ctattgcaga taagaaagtt caagcctaag attaagcgtg gtatcgtggt
    11101 aatagtcaat aaaaaagcac gtccacttgt gctagttact cgcctattga ttttatagat
    11161 ttattagccc tttcgcgggc tttttatatt gatttttatg agaaatgaag aaatcagact
    11221 tttaagaaaa tcactgatac caagggttta aatgaggtaa tatggtataa ttaggacata
    11281 aaataatttt gtggtaagat ggtagtatct attttagcat atttccgagc aatcggggcg
    11341 attaaagagt cgcatagaaa gaggacaaaa tggcaacata cggattttta gatgttttag
    11401 aggaagagtt ggacaagaac tttccctttg actttgagat tagctgggac aaacgcaacc
    11461 atgcggtaga agtgagtttt ctattggaag cgcaaaatgc tgcaggtgtg gagatggtgg
    11521 atgaagacgg agaggtttta tcagatgaca ttctttttga ggaagcggtc cttttctaca
    11581 atcctgccaa gtcaacagtc aatgaggaag actatttgac tgttattcct tacctgccta
    11641 aaaaaggatt ttcccgtgaa tttttagctt attttgcgct tttccttaaa gatactgctg
    11701 aggttgggct agatgtcctc atggactttt tggaagaccc agaagcagag gaatttgtca
    11761 tggaatggaa ccaagaagtc tttgaagaag gaaaagttgg cttggaagag ggagaatttt
    11821 atccttatcc aagatactag gagttggttg gaggtgttat gaagaaaatt gggacttatt
    11881 tggtttatgt gctagctttt gtctttatta tgctagcttt tgcttgtgga acaatcgcat
    11941 ttgcagagtt ggggtattcc gcagttttag cctttacttt tggttatgcc ttcgctcttc
    12001 taagcatgta tttaatcttt attcttcatg agctgggtca tgctttttgt ggctacttga
    12061 caggctatcg gctggtggct tttggattag gacattttat tttgaccaaa aagtcaggca
    12121 ggtttcatct tagtagaaca gccattctga aaaatgttgg tgctcaatac attggtttaa
    12181 aagaggatga aagcgatcaa agaatcatcc tgatgctttc aggaggcttg atggttcatc
    12241 tcggcttgat attattggcg atagtgtttg gatttttgac aagaagctgg tattttgcag
    12301 ggacttggat ttttcttaat ttatctttct tcctaaataa cattttgcca gtcgacatca
    12361 ccgatggagc aaaaatttgg gaattactac aacaccctga aaatagcaaa tacgcctacc
    12421 tggtattgag acattctgct caaactttgc tggctcctca agaatatgat ttgaaagact
    12481 ttgttcaagc agttcctgag gatgcacgag ggagttttgc ggatggtgta ttaggtatgc
    12541 aaggtgaagt ttccattttg gaagggaagg aagaagtagc taaacagcag tttcaagcat
    12601 tacttgagag gacaactact ccgatgatgc agacagttgc ccaactatcc ctacttcata
    12661 tcgccttgtt ggaaggagat tttgagcaag cggagcaata tgctagtatt cgacaggtca
    12721 aatcgtttct gtctctcaaa ctgtccaatc ttcaagtgat acaagcttgg tatcaattta
    12781 aggtaaggaa agatgtggtt caaactcaca aggctatgaa gattgctaga cagaaaatgg
    12841 atagtagtcg aatgttgagg gacgagaaac gctattatga aaacttgtta gcagagctgg
    12901 aaaaggaaat gttagaagga actgaagatg gaactagaaa tttctgattt cccaggctgt
    12961 aagattgctt tgttttgtgg ggataagctt ttgactatct tacgcgatga taaggcaagc
    13021 attccctggg ccaatatgtg ggaactgcca ggtgggggac gtgaaggtga tgaaagtcct
    13081 tttgagtgcg cggcgcgtga agtttatgaa gaactgggca ttcatttaga tgaagactgt
    13141 ctgctttgga gcaagattta tcctagcgta atttttaaag gcaagaaatc gctctttatg
    13201 gttggtcagc taagacaaga acaatttgac aatatcatct tcggagatga aggacagggc
    13261 tataaattga tgcctattga ggaatttctc aattctaaac aggcagtgcc tcagttgcag
    13321 ggaagattga gagattattt ggaggaaagt ttatgataac acttgttcga gcgggagcag
    13381 aggatttaga aactattatt gcaattcaga gagccagctt taaggctgtt tatgaaaaat
    13441 accatgatga atatgaccct tatctcgagg actgcgaacg aattaagtgg aaattagtcg
    13501 agcgtcccaa tagctattac tactttgtaa aagaagatga agaaaacatc ggatttttac
    13561 gagttcagac caatgccgag ttaacgaaag cttggttggg aacggcagcg attttgcctc
    13621 agtatcaagg aaaagggtat ggatctaaag gtttaagctt actggaaaaa gaatttccaa
    13681 ccatcacaca atgggatttg tgtacggtgt tacaggatgc aggcatggtt gctttttacg
    13741 agaaaaacgg ctatcgtcaa atccacattg aggctgaaaa agaaggcatg gatatggtct
    13801 acatgaaaaa attgatagag aaatagaaag gatggttcag ttaaatttct aaactgaacc
    13861 cgccctaaac actgtgccaa aaagataaac ttctcttaga cacaagcgtc ttcagagaat
    13921 ttcctatttt ggttttgtgt tttacgggct tggtatctta atgatggaaa catggcaaga
    13981 gttaaaagtt acagtgaagc gtgagggaga ggagttggtt tccaatctct tgattgagct
    14041 gggagcgcaa ggtgttgcga ttgaagacag tctggactat gtgggaaatg ttgaccgctt
    14101 cggcgagatt ttcccagagg tcgagcagca agaagaaatc gtagtgacag cctactaccc
    14161 tgacacggtg gatgtgacag tggttgaggc ggacttgcag gctcgcatat cagaattgac
    14221 ggattttatg gatttgggag agttaaaaat aggcacgact gccttggctg aggaagactg
    14281 ggctgacaac tggaagaagt attatgaacc ggcacgcatc acccatgacc tgaccatcgt
    14341 accgtcatgg acggactatg aggcaacagc aggagaaaag attatcaagc tggatcctgg
    14401 tatggccttt gggacaggga cccacccaac aaccaagatg agcctctttg ccttggaaca
    14461 ggttcttcgt ggtggcgaaa cagtgctaga tgtagggact ggttcagggg ttctttctat
    14521 tgcaagctca ctcctaggtg ccaaggagat tttcgcctat gacttggatg atgtggcggt
    14581 tcgtgtcgct caggaaaata ttgagctcaa cactggtatg gaaaatatcc atgtagcagc
    14641 aggagacttg cttaagggag ttgagattga agcagatgtc atcgtggcta atatcttggc
    14701 ggatattctc attcatctga cggaggatgc ttatcgcttg gtaaaggatg aaggctacct
    14761 catcatgagt ggcattatca aggacaagtg ggacatggtg cgccagtcgg ctgagtcagc
    14821 tggatttttc ctcgaaactc acatggttca aggggaatgg aatacctgtg tctttaagaa
    14881 aaccaaggat atctctggtg tgattggagg ctaggatgca acagtatttt gtcaagggga
    14941 gtgcagtctc tcctgtcacc atcgaggaca gggaaaccag caagcatatg tttcaggtta
    15001 tgcgcttgaa agaagatgat gaggttactt tggtctttga tgatggaatt aagcgcttgg
    15061 cgcgtgtgct ggatgtggaa gctcgtcagt ttgaattggt agaagaacta gatgacaatg
    15121 tggaactgcc cgttcaagtg accatttcat ctggttttcc caagggagat aagttggagt
    15181 tcatcgctca aaaagtaacc gaactgggtg ctagccaaat ctgggccttt cctgcagact
    15241 ggtcagttgc caagtgggat ggcaagaaat tgggtaaaaa agttgaaaaa ctagaaaaaa
    15301 ttgcccttgg agcagccgag caaagcaagc gtaatattgt accaagtatt aaacttttcg
    15361 agaaaaaagc agattttcta gctcagctgg accagtttga ctctatcata gtagcctatg
    15421 aagaatcagc taaagaagga gaagccgctg cgcttctgca agcagtcact ggtcttgaaa
    15481 caggagccaa actgctcttt atctttggtc cagaaggtgg tctctcacct gcagaaatcg
    15541 aaagttttga agctaaggga gccgttttgg taggcctagg tcctcgtatt ttgcgagcag
    15601 aaacagcacc gctttacgcc ttatcagccc ttagtgtttt agtagaatta gagaaataag
    15661 aggaagaaaa tggaacaaaa acaccgttca gaatttccag agaaggaact ctgggactta
    15721 acagccctat accaagaccg tgaggatttc ttgcgtgcaa tcgagaaagc tcgcgaagac
    15781 atcaaccagt ttagccgtga ttacaagggc aatcttcaca cttttgagga tttcgagaag
    15841 gcctttgcgg aattggaaca gatctacatt cagatgagcc atattggcaa ctatggtttt
    15901 atgcctcaga cgacggacta tagcaatgac gaatttgcaa atattgctca agctgggatg
    15961 gaatttgaaa cagatgccag cgtagccttg accttttttg acgatgcctt ggtggcagca
    16021 gatgaggaag tcttggaccg tttgggtaaa ttgccacatt taacagctgc cattcgtcag
    16081 gctaaaatca aaaaagccca ctacttaggg gcagatgtgg agaaggcctt gataaatctc
    16141 ggtgaagttt tctacagtcc gcaggacatt tatactaaga tgcgagctgg ggattttgaa
    16201 atggctgact ttgaagccca tggcaagacc tacaaaaaca gctttgtcac ctatgagaat
    16261 ttctaccaaa accatgagga tgctgaggtt cgtgagaaat ccttccgttc cttctcagag
    16321 ggacttcgta agcaccaaaa tacggctgca gcagcctatc tggctcaggt caagtctgaa
    16381 aaactcttgg ctgatatgaa gggatacgac tctgtctttg actatcttct agctgaacaa
    16441 gaagtggacc gtgtcatgtt tgaccgccag attgacctca tcatgaagga ctttgcacca
    16501 gtcgctcaga gatacctcaa gcatgttgcc aaggtaaatg gtcttgaaaa gatgaccttt
    16561 gcagactgga aattggactt ggacagcgcc ctgaatcctg aagtgactat tgacgatgcc
    16621 tatgatttgg tcatgaagtc ggtagaacct ttggggcaag aatattgtca ggaagttgct
    16681 cgttaccaag aagagcgctg ggtggacttt gctgctaaca gtggcaagga ttccggtggt
    16741 tatgcggcgg acccatatcg cgtacaccct tatgtactca tgagctggac aggccgtttg
    16801 agcgatgtct ataccttgat tcatgaaatc gggcattctg gtcaattcat cttttcagac
    16861 aatcatcaaa gttacttcaa tgcccatatg tcgacctact atgttgaagc accgtcaacc
    16921 ttcaatgaat tgctactcag tgattactta gagaaccagt ctaatgaccc acgtcaaaaa
    16981 cgcttcgctc tggctcatcg cttgacagac acctacttcc ataatttcat cacccacctc
    17041 ttggaagccg ccttccagcg taaggtgtat acattgattg aagaaggaga gacctttgga
    17101 gcaagcaagc tcaacagcat tatgaaggaa gttttgacag atttttgggg cgatgccatt
    17161 gaaatcgacg acgatgcagc tttgacttgg atgcgccaag ctcactacta tatgggcttg
    17221 tatagttaca cttactcagc aggactagtt atctcgactg ctggttacct tcatctaaaa
    17281 cattctgaaa ctggagctga agactggctc aatctcctta aatcaggtgg tagcaagaca
    17341 ccacttgagt cagccatgat tatcggtgct gatatttcaa cagacaaacc actccgtgat
    17401 accatccaat tcttgtctga cacagttgac cagattatcg cctatagtgc tgagttgggg
    17461 gagtaagggg aatagaaaaa ttttctagaa tgatgtttat gggcttgtta agcgtcattc
    17521 tatttgtctt agttgtagct attttcaaaa atgtcgttga aggtcgtgat gttaaatttg
    17581 aacttactgt agaactggta ttgatagtta tttacgtcat tttatatcag attgtattta
    17641 attgagaatt caatattgag tcatttaaga ctgccttttt gaacagttgc tagaaatgta
    17701 gaggtattga ccatgtatca agatttacta agaaaaatag cagaagaaaa accaaattat
    17761 aaccaggagg aaatccagtg gttgcttgat catttaggag atccttctcc agaaattcgc
    17821 gatgaccttg tttttacaag ctttgctaga gggattcagg aagagctatt tacacaggaa
    17881 caatttcatt tcattgttga aggggtttca tctgatgtag gactagataa agagattgat
    17941 aaggtaggcc tgccaacact ggaacgttct tttagggcac ttgtttatgc aaatctcttg
    18001 tctgcagatg ctaaccagca atcggttttt tatcagggat tacaatcaga aattcgtaat
    18061 gtccttttaa atcaaggctt gcactatctt tcaaaagaaa aggatacaac aggtttctca
    18121 agtcagtatg gttgggttca tgcttttgca catggagccg atttactgac agaggtggtt
    18181 tgtcatccag actttcctaa aaacagagtt catgaagtat ttgatatact tggccaacta
    18241 tttaaaagaa tgtcgattcg ctttacagat gatgaggatt ggcgtttagc aagagtgatc
    18301 tatgaaccta ttttacaagg gaagttggag caagaacaag tagcttcttg gataaaaact
    18361 gttgattttc cgatagaaga aagggaggat ttttacaaat tttccaactt tagatcctgt
    18421 ctggtggaag tctatgtcca acttgaccag agaaatagtt tacaagatga cctgaaagaa
    18481 gctatccagt cttttcaata ctagggatta gctaatagtt ctatgatttt caaaaaattt
    18541 ccaatcaatt ttcttgaaac cctttcctac ttttgataga ctagtatcta gtttttgaaa
    18601 aaaggagaaa gaaaatgaaa aaatttgttg ctgagttaat cggtacgttc atgcttgtgt
    18661 tcgttgggac aggagctgtt gtttttggaa atggtcttga tggccttggt caccttggaa
    18721 tcgcctttgc ctttggtttg gcaatcgtgg tggcggccta ctcaatcgga actgtttcag
    18781 gtgctcactt gaacccggct gtttcgattg ctatgtttgt aaacaaacgt ttgtcatctt
    18841 cagaacttgt aaactacatc cttggtcagg ttgttggagc tttcatcgct tctggcgcag
    18901 tcttcttcct cttgggtaat tcaggtatgt caactgctag tcttggtgaa aatgccttgg
    18961 caaacggtgt cactgtcttt ggtggtttct tgtttgaagt catcgcaact ttcttgtttg
    19021 tcttggttat catgactgtg acttcagaaa gtaagggcaa tggcgcgatt gctggtttgg
    19081 taatcggttt gtcattgatg gcgatgattc ttgtcggatt gaagattact ggactttcag
    19141 taaacccagc tcgtagcttg gcaccagctg tcttggtagg cggcgcagcc cttcaacaag
    19201 tttggatttt catccttgca ccaatcgctg gtggagttct tgcagccctt gttgcaaaaa
    19261 atttccttgg aacagaagaa taattgaaac tcaaaaagcc ttgctcctca tcttgaggaa
    19321 cagggctttt tcgtatgata ctcttcgaaa atctcttcaa accacgtcag cttcaccttg
    19381 ccgtaggtat ggttactgac ttcgtcagtt tcatc
//
LOCUS       DAVKHZ010000038        18885 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000038 DAVKHZ010000000
VERSION     DAVKHZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.038"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(115..390)
                     /locus_tag="VWW99_001218"
     CDS             complement(115..390)
                     /locus_tag="VWW99_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9539063.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(496..1335)
                     /locus_tag="VWW99_001219"
     CDS             complement(496..1335)
                     /locus_tag="VWW99_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9539064.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1477..2325
                     /locus_tag="VWW99_001220"
     CDS             1477..2325
                     /locus_tag="VWW99_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9539065.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2418..3335)
                     /locus_tag="VWW99_001221"
     CDS             complement(2418..3335)
                     /locus_tag="VWW99_001221"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9539066.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3773..3978
                     /locus_tag="VWW99_001222"
                     /pseudo
     CDS             3773..3978
                     /locus_tag="VWW99_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4289..4582)
                     /gene="rpmA"
                     /locus_tag="VWW99_001223"
     CDS             complement(4289..4582)
                     /gene="rpmA"
                     /locus_tag="VWW99_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9539067.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4599..4943)
                     /locus_tag="VWW99_001224"
     CDS             complement(4599..4943)
                     /locus_tag="VWW99_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9539068.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4959..5273)
                     /gene="rplU"
                     /locus_tag="VWW99_001225"
     CDS             complement(4959..5273)
                     /gene="rplU"
                     /locus_tag="VWW99_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9539069.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5309..5396)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5461..6159)
                     /locus_tag="VWW99_001226"
     CDS             complement(5461..6159)
                     /locus_tag="VWW99_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9539070.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
     gene            6561..6860
                     /locus_tag="VWW99_001227"
     CDS             6561..6860
                     /locus_tag="VWW99_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9539071.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            complement(7202..8176)
                     /gene="pta"
                     /locus_tag="VWW99_001228"
     CDS             complement(7202..8176)
                     /gene="pta"
                     /locus_tag="VWW99_001228"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9539072.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(8220..9116)
                     /locus_tag="VWW99_001229"
     CDS             complement(8220..9116)
                     /locus_tag="VWW99_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9539073.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(9113..9931)
                     /locus_tag="VWW99_001230"
     CDS             complement(9113..9931)
                     /locus_tag="VWW99_001230"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9539074.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(9915..10586)
                     /locus_tag="VWW99_001231"
     CDS             complement(9915..10586)
                     /locus_tag="VWW99_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9539075.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            10714..11283
                     /locus_tag="VWW99_001232"
     CDS             10714..11283
                     /locus_tag="VWW99_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9539076.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQLTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            11396..12355
                     /locus_tag="VWW99_001233"
     CDS             11396..12355
                     /locus_tag="VWW99_001233"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9539077.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            12365..13480
                     /locus_tag="VWW99_001234"
     CDS             12365..13480
                     /locus_tag="VWW99_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9539078.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEANNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            13485..13832
                     /locus_tag="VWW99_001235"
     CDS             13485..13832
                     /locus_tag="VWW99_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9539079.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            13840..14055
                     /locus_tag="VWW99_001236"
     CDS             13840..14055
                     /locus_tag="VWW99_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9539080.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            14138..14779
                     /locus_tag="VWW99_001237"
     CDS             14138..14779
                     /locus_tag="VWW99_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9539081.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGRALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            14797..15486
                     /locus_tag="VWW99_001238"
     CDS             14797..15486
                     /locus_tag="VWW99_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9539082.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(15483..16163)
                     /gene="radC"
                     /locus_tag="VWW99_001239"
     CDS             complement(15483..16163)
                     /gene="radC"
                     /locus_tag="VWW99_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9539083.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(16206..18497)
                     /gene="pcrA"
                     /locus_tag="VWW99_001240"
     CDS             complement(16206..18497)
                     /gene="pcrA"
                     /locus_tag="VWW99_001240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9539084.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQTMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESRARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(18688..18885)
                     /locus_tag="VWW99_001241"
                     /pseudo
     CDS             complement(18688..18885)
                     /locus_tag="VWW99_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5778 a         3981 c         3453 g         5673 t
ORIGIN      
        1 tacaatattt tcggaacatt caacttttta actctattta ttactagatt tcataattaa
       61 aaagcctact gaccaagcta gaaagcttga tacaataggc tttttaaaga ctgattattt
      121 aacagcgtct ttaagagctt taccagcttt gaatgctggt actttagaag ctgcaattgt
      181 catttcttta ccagtttgtg ggttgcgacc tttacgttct gcacgctcac gaacttcaaa
      241 gttaccaaaa ccgatcaatt gaactttttc accagctgca agatagtcag ctactgctgc
      301 aaatacagct tcaactgctg ctgctgagtc tttcttagtc aattctgtag cttctgctac
      361 tttagcgatc aaatcttgtt tgtttgccat gttaatgatt cctccagata atttctaatt
      421 aacaaatata atcatatcct aaaatccctt ttaggtcaag ttaaaaacgc tatttcttcc
      481 aattttattt attttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt
      541 gtgagtttga ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg
      601 taagctttct ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc
      661 tgctacagca cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt
      721 agttccacga cctttaacca ttggctgcaa ttcatggtct ttcatttgca tgacaacacg
      781 gatattgaga agggagctca acaagccagt tacacggcca attcgtccac ctttgacaag
      841 attttccaaa gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg
      901 agataaaatt gcctccatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg
      961 gaatttcagg gcttggtcag tgaaggaact atcaacaaca atcacatctg cagtagatag
     1021 gctagcacct tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc
     1081 aagaatctgg ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg
     1141 tggctgactt gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc
     1201 ttctttcaaa tccgcatcag aataaacaac attatcaatc attacagata atggaacaat
     1261 tgtaatatct aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat
     1321 cttaattttt gtcatagtat caatctttct attttaggat tcagattggt ttccttactt
     1381 ttaattatat caaaaaaaag attaaaaatc ctaatggagt caatcaaatt ttccgtaaaa
     1441 tttgatataa tcaacttata agaaaagagg tgtcctatga ttaaaaaaat ttaccccatt
     1501 tttaccattt tactaggtgc tgctatttat gcttttggac tgacttattt tgtagttccc
     1561 catcatctct ttgaaggagg ggcgacaggc attaccctca tcacctttta tctttttaaa
     1621 atccctgttt ccctcatgaa cctgctgatt aatattcccc ttttcatcct agcttggaag
     1681 atctttggag ccaaatccct ctattctagt ttactaggaa ccttagcttt gtccgcctgg
     1741 ttagcttttt ttgagcatat tccccttcat attgatcttc aaggtgattt actaatcaca
     1801 gcccttatag cgggaatcct attgggaatt ggccttggaa ttatttttaa tgctggaggt
     1861 acaactggcg gaactgatat tctagctcgt attctcaaca aatacactca tatatccata
     1921 ggaaaactgc tctttatctt agatttttgt attctcatgt tgattctcct aatcttcaag
     1981 gatttgagat tggtttccta cacgcttttg tttgatttta ttgtttctcg tgttattgat
     2041 ttgattggtg aaggaggata tgccggcaaa ggctttatga ttatcacaaa acgtcctgac
     2101 caacttgcta aggcgattaa tgatgacctc ggaagaggtg ttacttttat ttctggtcaa
     2161 ggctactata gtaaagaaaa tttgaaaatc atctactgta ttgtcggaag aaatgaaatt
     2221 gtgaaaacga aggaaatgat tcatcgaatc gatcctcaag cctttataac tattacagaa
     2281 gcccatgaaa tcctaggaga aggcttcacc tttgaaaaag aataaaaaga ggtaatgtcg
     2341 tgacctcaaa agttagacta aatcatctat cttttgggtt acagacaacc tcttttttgt
     2401 tttatttact caagctctta agaccaattc cgagttactt cttcatcagc ctttaactga
     2461 tccactaatt ggtcaactga gtcaaatttg gtcatatcac gaatgcgatc aagccaataa
     2521 accatgacgg tttccccata aatatcttga ttaaaatcaa aaatattgac ttcaaaacgt
     2581 gcttcttctc catcaaaggt cacatttttc ccgacactag ccatagcacg atacttctgt
     2641 ctttgaatct caacatcaac gacataaacg ccatctgctg gcatataagt acggtctaaa
     2701 agcactaaat tcgctgtcgg ataaccaatt gtacgaccac gagcattacc atgaactacc
     2761 atacctcttg atggaagcgg tgcccccaaa agttttcctg cttctttcac atttccatct
     2821 aaaatagctt gacggatacg agttgaacta atctttcctt tctcatcttc tacaggtgga
     2881 acaatgataa cttctccatc aaagtaatcc tttaaatctt ctgctgtttt tttgtcagaa
     2941 ccaaatgtat aatcaaaacc tgcaacaata attttggcat tcatagcctt gatataagtt
     3001 gcaaagaatt cttgtgcagt gagactagcg aattgactac taaaatcaag gagatataat
     3061 tcttctacac cttcgcgctt taattttctt tcacgttcag cagggttcaa aatatgcaaa
     3121 aacaaatctg gatgataagg ctctaaagcg atctttggag attcattaaa ggtcataacg
     3181 acgataggca ataaatcctt tctcgcagcc ttgttggcaa cacgaaataa ttcttgatgc
     3241 cccttatgta tgccatcaaa atagccgaga acaacgactg aatcagatgg tgtgccaata
     3301 tctttttggt tttttatagg aatagtaata atcataaaat aattatatca tagcgatagc
     3361 tatttctgga acagaaaatc tgaaatgttg tttttttcac atgaagtgta cctgttttca
     3421 aaaagcactt tattctatcg ttgcttaact atgaactttg caatattctt ctcaaaaact
     3481 tgtaggacat cttcaaaatt ttgcaaggag tgattagact tgttcggtaa ctataaagtg
     3541 tcatactatg cttatgtatg aaaaagcaat gcaactaact cctgagaact ttaaattact
     3601 aattggtgcc gaaaaggtag aatttagaat agaggtacac ctatggctgt aaaatttaca
     3661 aaatgagaca acttgggcaa gatgtttgaa gaatttccta aactccctga tttgaagcaa
     3721 gtcactttcc ctgatgacaa agaaaaaagc caaagcaata aagaaaaact agatgactgc
     3781 tttccaacaa ctcccatcta gtgtgcttca gactgggcta tttttctctc catctgttag
     3841 cttggattct cagaccgttt cagctaaaga atatcttttc ctttatcaga aggaacggct
     3901 caagccattc agacaagtga agggacgaca agccaatatt tgaaaccaga tagcagttct
     3961 tatagtcaat tgaaataaaa tctgaagaaa tcgagtagga aactcaaatc aatttttagc
     4021 agtgttctat tccagattca tactcaatga aaattaaagt gcaaactagg aagttagccg
     4081 caggctgtac ttgagtacgg caaggtgaaa ctgacgtggt ttgaatttga ttttcgaaga
     4141 gtatcaatcc actatacata ttaaaaagta tgggtatcaa aataaaaaaa caggctctcc
     4201 gaaaactcag aaagccttat tttaagctgt ttctagtttc ctcaccttta catttcaagg
     4261 ttcgggataa aaggttcaat ggaccttttt atttagcgat tgggtaaaca gaaacttgtt
     4321 ttttatcgcg tcctttacgt tcaaagcgta ctacgccttc aactttagcg aacaaagtat
     4381 cgtctccacc acgaccaacg tttacacctg gatagatgtg tgtaccacgt tgacggtaaa
     4441 ggattgatcc acctgttaca gtttgtccgt cagctgcttt agctccaaga cgttttgctt
     4501 gtgaatcacg tccgtttgat gtagaacctc cacctttttt gtgggcgaaa agttgcaagt
     4561 tgttaagagt catttttaac ataatgtttt cctccgtgtt agttttctgt gataactctg
     4621 gtttggacga actcagaaga gttctccgat aagtttgcca tacctaagaa aaatgattca
     4681 aagaataact gggtcatttc tctctggtgt gaaggaagat cttttggtat ttcaaccatc
     4741 agatagccac cttcatcttc gtttaattct aggattggtt catagcctgc aaatttctca
     4801 atagaattga taaagttaat ggcaagcgta gaaaccgatg cacacacgac atctaagccg
     4861 tattcgccac tctcggcgtg tccagtaatt tccgcactcc tcagctcgcc atcttcggct
     4921 ctctcaaaga ctgcctgtat catgtgttct ccttaaaatt aagcgttgat tgcgttgatg
     4981 acaacttttg tatatggttg acggtgacct tgtttacggt ggctaccttt tttaggtttg
     5041 tacttgtaag taaccacttt cttttgtttt ccttgttttt caacagttcc aactacagta
     5101 gctccagcaa caagtggagt tccgacaaca gtgttttcac caccaacaag aacaacttcg
     5161 ttaaaagtaa cttcttgacc agcttcaacg ttcaattttt caacgtaaac tgcttgacca
     5221 acttcaactt taacttgttt tccgccagtt ttgataattg cgtatgtgct cattatgcac
     5281 ctcctatgat ttttatgggt ttccccgatt ttttgtgaag actcgcctag catcgtggga
     5341 cgaaccactt aaacttgaat aatcaagcaa cgatgttcgt gcggttgcac aggatcgtgc
     5401 atagtcaact cttcaagtat agcatatctc ctattttctt acaagtaata acacctaaaa
     5461 tcaagctttt tcttttactt ttttctgcca agaggcaaaa agcatgctga ggtaaaaaac
     5521 gctcatcata ataggaacac caagaatggt cttttcatga tagaaaatcg tcaaataggc
     5581 tgaaaagaca acgccaagga caaaactact aagcaggcta acaaatatga atccttcacg
     5641 caaaaaagga gtgtgcttgg ttcggaaata atctccaaaa gccagcatgg tccgtttgat
     5701 attccctgtc ataaaagcgt tattataggc aatacccgac acttctccaa aagcagttgt
     5761 caccagtccc atacagaagg ccaagggcgg cactagatag atattatcca cagtttgcgg
     5821 cacaaaagca ataatgattg ataagattgc caagggaatc aaggacagaa taggtttttt
     5881 cacaattctc aatttttcct tataaatcgt taataaaaag actcccatca taaacgctag
     5941 caaggtgaga accttgtccc taacatccga aacattattt ttaattaatt ctactgaaag
     6001 aaagacaaca tttccagttt gtccagctac aagggtattc ccgcgaacaa taaaagtgta
     6061 agcatccaca tatccagcac aaaacgtcaa aaaaagtgct aaccttttag actgacgtga
     6121 tatttttctt ataggtaata acctcatttt acctcccatt gtattttctc ttagaaatat
     6181 tgtaccattt tctttctaaa aaatcgtagg ctaccattta gattttacta ttagcataaa
     6241 aataataata gacaactatt tatccaaaaa tagatagatg taacatgttt gcaaacaaag
     6301 catacgaacc tttagtaaaa tcatttccat gaaactagaa tagagccctc ttagcaaaaa
     6361 tcattatttt aatttatttc taatcactcc ttgacataaa taactctcac caataaaaga
     6421 ctatgtctta aaaaaatggt ataataaaat caatacttgg gcttgatggc tctgctacta
     6481 ataacaatta ggagagaaaa tcaggcactt gttaacaaca aggattatcc ccttgagatg
     6541 aaaggaactt tagaaatctt atgatgaaca tgcaaaacat gatgcgtcaa gcacaaaaac
     6601 ttcaaaaaca aatggaacaa agccaagctg aacttgctgc tatgcaattt gttggcaaat
     6661 ctgctcaaga tcttgtccaa gcgaccttaa ctggcgataa gaaagttgtc agcattgatt
     6721 tcaatccagc tgtcgttgac ccagaggacc ttgagactct ttctgatatg accgttcaag
     6781 ccatcaactc tgctcttgaa caaatcgatg aaactaccaa gaaaaaactg ggtgctttcg
     6841 ctgggaaatt acctttctaa aaacaaggag ctagaacaat gcttgtcgat aacaaaggct
     6901 aagaaaggtg caaaaattat aaaaaacgca taatagcagg tgtcaaagca ctcaatatta
     6961 tgcgttttct catgaaaaaa ttctcttttc atactcattt aaaatcaaaa agggaattta
     7021 tcattttacg gacatgtata ttacagattt ctataggtcg tatatagaca ggatattatc
     7081 gcttagaagt tgtccttttt gacaccaatc agtaatttag ttctcaggag ttaatggaag
     7141 tgcttttcgc acatagtttc ataactacag tatagcatag tatatcactt tatagttttc
     7201 actattgatg aactgcttga gctgctgtga tgagggtcaa cttgtaaaca tcgtctgcat
     7261 tacatccacg agaaaggtcg ttaactggtt tgttcaaacc ttgcaaaaca ggtcctacag
     7321 ccgcaaaacc acccaggcgt tcagccatct tgtaaccgat atttcctgcc tcgatacctg
     7381 ggaagatgaa gacatttgct tgaccagcta ccgtacttcc cggagctttc agagctgcag
     7441 tttcgggaac aaaggccgca tcaaattgca actcaccatc gatttcaagg tcaggacgca
     7501 agtcgtgagc aattttagtt gcttcaacga ccttatcaac gctttcacca aaccctgaac
     7561 ctttagtaga atagctcaac atggcaattt taggttcgat gccaaacatc ttagctgtga
     7621 ttgctgagtt gatggcaatt tcagccaagg cttctgcatc tggattgata ttaatggcac
     7681 agtctccaaa taggtaacgt tccgtaccac gaaccatgag gaaggctcct gaagtacgag
     7741 ttacatttgg acgagttttg atgatttgta gagctgggcg aactgttgaa gctgttgagt
     7801 gaatcgctcc tgacaccatt ccatcaacca agcccaagta aaccaacatc acaccaaagt
     7861 agttgacatc ttcaaccaaa accttgcgta catcttcttc agtcattttg cccttgcgac
     7921 gctccaccaa ggcagaaacc atttcttcaa attgaggata atgttgaggg tcgatgacct
     7981 cataaccatc catgattcct tcaatttcaa gataaatttt aattttttca ggatttccaa
     8041 gcaaaacagg aatcacttct gtttctttta ctaagcgttt tgttgcttga agaatacgag
     8101 gctcttcccc ttcagggaga acgatacgag catttttacc aacaaggttg gctttgagac
     8161 tttcaaaaac ttccatgagt tttctccttt aagataataa ttatactttg agacagtttt
     8221 tatagagtat tagttgataa ctgggtaata aggttactga aatcatccgg caagggactt
     8281 tctaactgca agtcttgctc taaaaatgga tgataaaagg ataggtaatg gcaatgcaga
     8341 gcctgacgtt gaataccatc ttccagacta ccaccataca aatcatctcc cagcaaagga
     8401 aaaccgatat gagaaaaatg gactcggatt tgatgggttc gaccagtgtg caggtgaata
     8461 tagaccaagt gaatatttcc ataagaagct acaatcttgt atgaagtatg ggcatacttt
     8521 ccgcctttag ccactcgtct ggtaataatg gaatcttcat cacgcgcaat cggagcaata
     8581 atttcccctt ctggctccaa atgtccatct cccttaacca aagcaaagta gcgtttctcg
     8641 atagatttct tctgcaactg cttgtctaat cgtgcatggg cataaccgtg cttggcaaag
     8701 agcatcaagc cagaagtgtc cctatctagt ctggtaacaa tgtgaacctg ctgattttca
     8761 taattttgct tgacatagta acccttgata aaattggcaa tggtattaga gtgattgaca
     8821 ctaggaatag aagccactcc atagggttta ttcaagacta gaaagtggtc atcctcatag
     8881 agaatatcta atggatgctc aatagcctcc aaggtttcaa agccttcctc agcgggaatg
     8941 taaatggtaa cgtagtctcc aacgtccaat agatacgttg cattttgcgg ttgattattg
     9001 accagaatag ctccacctcg aaacttaatc ttggccagca atcccttaga aacctcgtgc
     9061 ttttttaaaa aggtcttaac cttgacatgt tcatctgcga taaattcaaa cctcattcat
     9121 ccacctcacc gataaaggca tccttaacac ggttccagaa actggtatgg ctaggagtcg
     9181 cgacaaagtg aatcttatga tggtcgattt gatactcaat acgctcaata ttacggaaag
     9241 aataaacgct attgtcaacc gaaatagtat gataatcgtt tcttgttgga ataagttcaa
     9301 tcttatcctt cttaggcaca ataatggaag agcccagtgt tcgatagaca cgattattaa
     9361 ggctggcaat ctccgttaat tgcaaagctt caatggtagg gtgtaaaaca gcaccgccaa
     9421 gagacttgtt ataggcagta ctaccagtcg gtgtcgaaac tgttagcccg tctccacgaa
     9481 aacgttcaaa gggaacacca tttattacaa tatctgccac catggttcga tcagacctgc
     9541 ggatgctggc ttcgttgagt gctctgaaaa tcttaacttc accattttcg agaaagacct
     9601 tcacattcag aacagggtaa gaaacccttg ccccagtatc tagctgcaaa ttagtcacta
     9661 gcttgtccaa ctcaaaatca cgataatctg tatagaagcc caaatgtcca gtatgaagac
     9721 cgataaagcg gaccttgtca agctgatttt cgtacttatg aaaggccgac aagagcatac
     9781 catccccgcc aatggaaatg acaatatccg gattggtatc attgagtata aactgatttc
     9841 tcttcaaacg atctcgcaat tcatacaaaa ccctttgact ctgcggtttt ctattggcta
     9901 tcagatcaat tcgtttacct gtattcttca tctgtatcgt cactgtttcc tacaccgtca
     9961 tttaattttc tactcaaagg atcaaaaagt gcctgggctt cttggatatc atcacgaatt
    10021 tcacccattt cttcatccaa ctgatgggcg attctagctg taatttccag tcgcttctta
    10081 atctcatctg ggaaatcccc ttggtacttg tagttgagag aatgttctat cgttgcccag
    10141 aaattcatgg ccaaagtacg aatttgaatt tctgccaaaa tagtcttagc tccattgatg
    10201 gtatcaaccg tatattctac taccacatga taggaacgat agcctgatgc ttttctatga
    10261 gtaatgtaat ctcgctcctg tatgattcgc atatcctgac gcttgtgcaa aatatccact
    10321 acttccttga cgtcatctac aaactgaacc atcacacgta agccagcaat atcctgcaaa
    10381 tcgtgttcca aggtcgcata agtaatgcca cgacgagcca ttttttcttt gatgctctca
    10441 attggcttga ctcgaccggt cacaaactca attggagaat gcttattttg cttacgatat
    10501 tgcttacgaa taccacgaag tttaatcttt aactcaccaa cagcttgaat gtaaggatct
    10561 agaaattctt cccattctaa ggtcatatat tctcccttgt tctcatatta tccgtcaaaa
    10621 atatctttga ttttttctcc agattcatat acaataaata caatgcttat tataccataa
    10681 atccgctttc aacgataaaa aaaggtaaga aaaatgaaac atttagaaat tgaattgaaa
    10741 acactattga aaaaagatga atacaatcgt ctaaaagacc agctcacagg tgtcactcct
    10801 gttcttcaaa caaattacta catcgacacg cctgattttg aactgcgaga aaagaaagtc
    10861 gctatgcgca ttcgaacctt tgaagactgg gctgagttga cactcaaagt cccgcagagt
    10921 gttggaaaca tggaatacaa ccaaaaattg caactaaaag atgctgagaa ctatctgagt
    10981 aaggaagaac ttcctcaagg gctagtactg gatgaattgg cgaaacatgg tatccaaagt
    11041 aagaactggc aggttcttgg ttgtctaaca acgcttcgct atgaaatgaa aacagctatt
    11101 ggtctcatgg cactggatca gagccgatac tttgatataa cagattacga attggagctt
    11161 gaagtggaaa atcatgagca aggcaaacag gatttccgac aatttttaga gaaaaatcag
    11221 atttcctacc aaaaagctcc ttcaaaattg gttcgatttg tcaaaagtat gaaaaatagc
    11281 tgaaataatc tccatttttt ggtaaaatag aaaagataaa aatacacaaa tcccagttca
    11341 tatatgtaag gtaaatataa ctaggattta taaagtttac agaggacggt ctataatgtc
    11401 agatagaaaa aacatgaaac ttttcgcact caactctaac caagagattg cacaaaaaat
    11461 tgcccaagct gttggtgtcc cacttggaaa actatcatca cgtcaatttt cagacggaga
    11521 aatccaagta aatatcgaag aaagtgtccg tggttatgat gtttacatca tccaatcaac
    11581 aagtttccct gtcaacaacc acctaatgga attgttaatc atggtcgatg cttgtgtgcg
    11641 tgcaagtgcc cacagtatca acgttgtcct tccatatttt ggctatgcac gtcaagaccg
    11701 cattgcttgt cctcgtgagc cacttacagc aaaactagtt gccaatatgc tggttaaggc
    11761 tggagttgat cgtatcctga ctcttgattt gcatgccgtt caggttcaag gtttctttga
    11821 tattccagtg gataatcttt tcactgttcc cctattcgca aaacattact gcgataaagg
    11881 attgcttggt tcagatgttg ttgtcgttag ccctaaaaat tcaggtgtca aacgtgcgcg
    11941 tagcctggct gaatatcttg atgctcctat cgccattatc gattaccctc aagacgatgc
    12001 aactcgtaac gaaggttata ttattggtga tgttgaaggt aagaaagcta tcttgattga
    12061 tgatatttta aatacaggac gtaccttctc tgaagcttct aaaatcgttg aacgtgaagg
    12121 agctacagaa atttatgctg tttctagcca cggtctcttc gtcgagggag ctgctgagct
    12181 tcttgacaat actaatatta aagaaattct tgtgactgat tcagtagcaa caaaagaaaa
    12241 aactcctaaa aatgtatgct acatcactgc tagtgagtta attggcgatg ctatcgtccg
    12301 tattcacgaa agaaaaccag tcagcccact ctttgcctac aataaaaaga aataaggtga
    12361 ttctttgatt tatttggaca atgctgcaac gactcctatg tcagcagttg ctatttcagc
    12421 tatgaccaag gttatgcaag aaacccacgg aaatccttct agtattcatg gtcatggtcg
    12481 tcaagctggc aaactcttgc gagaagcccg tcaggaacta gcccagttac taaggacaaa
    12541 acctcaacat atctttttca cttctggtgg gactgaagcc aataatacta ccatcattgg
    12601 ctactgtctt cgtcaccaag aacaaggaaa acatatcatc acaactgcca tcgagcacca
    12661 tgctgtcctt gaaacaattg attacttggt tcaacacttt gggtttgaag caaccattat
    12721 ccagccagaa aatcaagaaa tcacagccca gcaaattcaa aaggctttac gtgacgatac
    12781 gattttggtt tctaccatgt ttgccaataa tgagacagga aacctactgc ccatcgctga
    12841 aattggccaa atactcaagc aacaccctgc tgcctatcat gttgatgcag ttcaggctat
    12901 tggtaaaatc ccaattcatt cagaagaatt gggcattgat tttctcactg cttctgccca
    12961 caaattccat ggtcctaagg gaatcggttt tctctacgca tctagcatgg actttgattc
    13021 ctatctacat ggcggagacc aggaacagaa aaaacgtgca ggaacagaaa atctgcctgc
    13081 catcgtaggc atggttgcag ccctaaaaga agacctagaa aaacaagaag aacattttca
    13141 acatgtacaa aatctagaaa ctgcctttct ggcagagctg gagggcattc agtattacct
    13201 gaatagagga aaacatcatc tcccttatgt tctcaatatt ggatttcctg gtcagaaaaa
    13261 tgacctctta ctccttcggc tagatttagc tggaatttca atctctactg gctcagcctg
    13321 tactgcaggc gttgtccaat ccagccatgt tcttgaagcc atgtatggcg caaattcaga
    13381 acgcttgaag gaatcccttc gcatcagttt gtcgccacaa aataccgttg aagacctaca
    13441 aaccctcgca aaaaccttaa aagaaattat cggaggttag ccatatggca tttgaaaaaa
    13501 tcattcagtt aaaaaattgt cgttacgatt acactcttag cccttctgtt aaaaaattca
    13561 ccctcaaaga taacaccttt tttgaaacta aggttggtaa ctatgaactg actcgccttt
    13621 tggaaaaagt gccaaacagc ggtgaaggct tccaactcaa aatcatcatt aacaaggaac
    13681 ttacaggggc taaaatcaat atcactgaca agtttggcct tcgtctagtt gatattttca
    13741 aatcagaaga ccaccatatt catcaggaaa aattctactt cctcatggat agcttggtag
    13801 aacgtggtgt ctttacaaaa tcggaaagat agagccaaaa tgtttgaact gacctataag
    13861 gactgctatc atgtagagcg tactctcaag tatgaggatc atgaggctct catgttgact
    13921 ttgtcaggat gtgtaacctt accagataca ctttatgtga cttctctgac ttttaggggc
    13981 caaaaagttt atcagggtct ggtcggagac ctctaccgtt ttctatctca tgcagatttt
    14041 ttacatcaaa actaagattt ttcttagttt tttcttattt ttgttgattt tttcacaatg
    14101 tttctataaa atagaagaat agaaaggttg tgattttgtg aaagataaac agtttgctat
    14161 tccaaaagct acagcaaaaa gactctctct ctactatcga atttttaaga gatttcatgc
    14221 agaaaagatt gaacgtgcca actctaagca aattgcagag gctatcggta ttgattcagc
    14281 gaccgtacgt cgtgattttt cctattttgg tgaacttggt cgtcgtggtt ttggctatga
    14341 tgtcaaaaaa ctaatgacat tttttgccga tttgctcaat gacaactcta ttaccaatgt
    14401 catgctggtt ggtattggaa atatgggccg tgcccttctc cactaccgct tccacgaacg
    14461 taacaagatg aagattatca tggcctttga cctagacgac catcctgaag tcggtaccca
    14521 aactcctgac gggattccca tttacgggat ttctcagatc aaggataaaa tcaaggatgc
    14581 tgatgtgaag actgctatcc taactgttcc cagcgtcaag tcacaagagg ttgctaatct
    14641 cttggttgat gctggtgtga aaggaattct cagtttttca ccagtccatc tgcatttacc
    14701 aaaagacgtg gtcgttcagt atgtcgattt gacaagtgaa ctccaaaccc tcctctactt
    14761 catgcgaaaa gaggattaga aagcgaaaat cattttatga aaaaaccagt tattgggatt
    14821 acaggaaacg aaaaagctca tccagatgat gacatcatga tgagctacgc agcaaaaggc
    14881 tttgttgaag gcgttaaaga tgctggaggg attcccatca tcctaccgat tggtgatcaa
    14941 gaaatggcct gccactatat cagtttgatt gacaagctca tcttgacagg tgggcaaaat
    15001 gtcgatccaa aattctatgg cgaacccaaa accattgata gcgatgatta tcaccttcaa
    15061 agagatatct tcgaactggc cctcatcaag gaagctatta aacagaaaaa gcccattttc
    15121 tctgtctgcc gtggtaccca actctttaac gttgccatgg gtggaacttt gtaccaagat
    15181 atcgaagacc attggcagga ttcttctgtt gagtacacaa ctcaacgctt ggtaacagaa
    15241 ccagataccg ttctccaaga aatctatgga gaaatctctc atatcaactc cttccaccac
    15301 cagagcatca aggacttagc accaaattta aaagttgtgg ctcatgatcc taaagatggt
    15361 atcattgagg ctgtcatgag tacggatgat gtcgcctttc tcggtgtcca atggcatcca
    15421 gaacttcttt ttgaaaatcg tcccaaagat aagaaactct ttgactatgt cgttaatgaa
    15481 ctttagatta aatctgtctt ttcacgataa ctaaagtaat tagaatgaga gacaatcaaa
    15541 tggtccaaga gaacaatccc catcagttcg caggcttctt taacaagttt agtgacatga
    15601 tcatcatttt ggctaggcgc taccgctcct gaaggatgat tgtggaccaa gataagagaa
    15661 gtcgccatat gcttgattgc atagtgcaga atctctcgcg gttcagcgat actacgagtt
    15721 acagaaccga taaaaatggt ctgctgatgg atgatttgat tttgagtatt gagatagagt
    15781 gccaccaggt gctcttgttt tttatcccct aattcctgct gcatcttctt ggccaacttt
    15841 tgactgctga gaatactttc catttcaaga gtctcgtgtt tgtgaatacg atgccccagt
    15901 tcaatcatag cttgtaattc tatggcctta acacgcccaa taccagacaa actctgcaat
    15961 tcctgcaggg tcattttttt caaatccgtt aggcttgaaa gattgttcaa gactttttgg
    16021 gcaatttcaa aaacgctagc ttgacgtgtt cctgtcctga gtaaaatagc tagcaactct
    16081 tggttactaa gcgcttcaac tccttccttg gccagccttt ctcttggtaa tagtgaatct
    16141 tcttggaatg aaatactgta cataaaaatc ctcctcactt tattattcgt gagaaggatg
    16201 gaaaattaga tttttttctc aattggagcc acactggcta aaagtttttt caaacctact
    16261 tctgggaaat tgattttcaa ttcctgccta gcaccgctac ctgaaacttc cagaacagtt
    16321 ccctctcccc atttcttgtg gagagcaatg tcaccaatgg accaatttgc ctcgctagat
    16381 gctgattttg cgccagctgt aaattgacca aatggaagac cgcttgactg gattgatttt
    16441 ggggcagcac cgcgtttacg gtcttgaaga gcctgagcca aactcatacc ttgaccaaag
    16501 gaaatactac cactgctata tgatgcctta aagcttgtat ttgcaggacg agccagacct
    16561 tgatactcaa gcaagtctga actgatttcg ttaataaaac gagtcggacg gttataattg
    16621 gtacgaccaa aaagcaagcg tgagttggca ttggtcagat agagaatttt ctctgcacgc
    16681 gtgataccta cataggctag acggcgctct tcttctaatt catctggatc ttcagtcgca
    16741 cgactaagtg gaaagacatt ttcttccatc ccaatcaaaa agacaactgg aaattcgaga
    16801 cctttggcag catgcagggt catcaaggtc acttctgatg tctcctgact acctgaatct
    16861 gtgtcggcaa tcaaagccaa gtcatttaag aaacgactca gtttgtccag accagtttcc
    16921 tcttctgtca catcagtggt gtcatcaaag ttcttcgtaa cagaaagaaa ctcttcgata
    16981 ttttcaaccc gtgccctgct ttctagagtc gcttgggcgt taagaatatc gacataacct
    17041 gttttttcta ggacggactc aaccaactct gtaatgctta agtggtctag ctgctcccgc
    17101 aaatcaagca tcatattggc aaaatcccag atagattggg ctgccttacc cttaatacca
    17161 gacaacataa tattagcaga agcatccagc atggacatag tttgcaaatt tgcaaaatca
    17221 cggattttct caactgtacc tagaccaatt ccacgtttag gctcgttgat aatacgctca
    17281 aaactaatat tgtcactcaa attagcaata aggttgagat aagcaataat atcgcgaatt
    17341 tccttacggc tgtagaattt ggttccgcca accatggtat aaggaatgtt agacttgagc
    17401 agggcttcct caattgtacg ggactgggca ttagtccgat agagaactgc aaaatcctta
    17461 tgaaggaagt tttgactgcg actaagttca tcgatggttc tggctacaaa tacagcctca
    17521 tccagctcat catcggcacg atagtaaacg atttgctccc catcagcgtt ttgagtccag
    17581 agatttttag gacggcgatt tttattgttt ttaataacct cgttggccgc ttggagaatg
    17641 gttttggttg agcggtaatt ttcctccaac aaaacaacct tggctttggg gtaatccttt
    17701 tcaaagtcca agatattctg catatcagca ccacgccaac cgtagataga ctggtccgca
    17761 tccccaacca cacagatatt tttaaaacgg gaagccaaga gtttgaccaa ttggtactga
    17821 gcgtggttgg tatcttggta ctcatcaacg tggatgtatt ggaatttttg ctggtagtag
    17881 gtcaaaacat caggattttg atcaaagaga cgcaaggtca gcataatcaa atcatcaaag
    17941 tcaacggatt cggactgacg aagttctttt tgataggctg tataacactg ggccacaatt
    18001 tgcgtataca tatcgccagc ttgggcagca taagcaacat catcaatcaa atcattctta
    18061 gcattggaaa tggtccccaa aatagttcgt tcattccatt ttttaggatc caagttcaac
    18121 tgttttagaa tacgtttcat gagcgttcgc tgttcaccag gatccacaat tgtaaaatta
    18181 cgattgtagc caatatggtc cgcatctcga cgcaaaatac gcacacacat ggagtggaag
    18241 gtcgcaatca gacagtcctg agtagctgga ttgaggctat aagcacgctc tttcatctca
    18301 cgcgcagcct tgttggtaaa ggtaatggcc aagatattcc aaggattgac cagcttttca
    18361 tcaatcaaat aagcgatacg gtgggttaaa acacgagtct ttccagaacc agcccctgcc
    18421 atgattagca agggaccttc tgtcgtttgc accgcctcag cctgacggtc attcattcca
    18481 tttaataatg cgttcatctt ctcttcctta ctcttgaagt caatatttct attatatcag
    18541 aaatcaggga aaatagaaac tttacgggga gtagcaaaat atagtggatt gaaactagaa
    18601 tactacactg tgggtttcta aaacactcga cttttcaccc aattttttca taaactagct
    18661 caatacctca atagacttcc tgcaaaacta gaatcctagt tcatgattga taataccagc
    18721 aatcaaattc attcgtaatc cgaagcgttt acgatgattt cgataagttg ttgaaaacat
    18781 tttaaacgtt tttactttgg caaagatgtt ctcaaccttg cttctctcct tagatagcgc
    18841 atggtttaag ccttatcttc aactgttagc ggcttgagtt tgctg
//
LOCUS       DAVKHZ010000039        18793 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000039 DAVKHZ010000000
VERSION     DAVKHZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.039"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            31..411
                     /locus_tag="VWW99_001242"
     CDS             31..411
                     /locus_tag="VWW99_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539085.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
     gene            605..895
                     /locus_tag="VWW99_001243"
     CDS             605..895
                     /locus_tag="VWW99_001243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539086.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            946..1638
                     /locus_tag="VWW99_001244"
     CDS             946..1638
                     /locus_tag="VWW99_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9539087.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1691..4102
                     /locus_tag="VWW99_001245"
     CDS             1691..4102
                     /locus_tag="VWW99_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9539088.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4352..5053
                     /locus_tag="VWW99_001246"
     CDS             4352..5053
                     /locus_tag="VWW99_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539089.1"
                     /translation="MAYIDMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5055..7763
                     /locus_tag="VWW99_001247"
     CDS             5055..7763
                     /locus_tag="VWW99_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9539090.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7859..8350)
                     /gene="tpx"
                     /locus_tag="VWW99_001248"
     CDS             complement(7859..8350)
                     /gene="tpx"
                     /locus_tag="VWW99_001248"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9539091.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8473..9402)
                     /gene="psaA"
                     /locus_tag="VWW99_001249"
     CDS             complement(8473..9402)
                     /gene="psaA"
                     /locus_tag="VWW99_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9539092.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9434..10282)
                     /locus_tag="VWW99_001250"
     CDS             complement(9434..10282)
                     /locus_tag="VWW99_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9539093.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10279..11001)
                     /locus_tag="VWW99_001251"
     CDS             complement(10279..11001)
                     /locus_tag="VWW99_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9539094.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            11301..13193
                     /gene="pepO"
                     /locus_tag="VWW99_001252"
     CDS             11301..13193
                     /gene="pepO"
                     /locus_tag="VWW99_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9539095.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPILSLLKEFQELETFADF
                     IAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(13316..13945)
                     /locus_tag="VWW99_001253"
     CDS             complement(13316..13945)
                     /locus_tag="VWW99_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9539096.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            14941..17163
                     /locus_tag="VWW99_001254"
     CDS             14941..17163
                     /locus_tag="VWW99_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9539097.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17193..17636
                     /locus_tag="VWW99_001255"
     CDS             17193..17636
                     /locus_tag="VWW99_001255"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9539098.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(17675..18090)
                     /locus_tag="VWW99_001256"
                     /pseudo
     CDS             complement(17675..18090)
                     /locus_tag="VWW99_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18109..18522)
                     /locus_tag="VWW99_001257"
     CDS             complement(18109..18522)
                     /locus_tag="VWW99_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539099.1"
                     /translation="MKKLYRIHFIAIAAIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFFLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISLWECL"
     gene            complement(18653..>18793)
                     /locus_tag="VWW99_001258"
     CDS             complement(18653..>18793)
                     /locus_tag="VWW99_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539100.1"
                     /translation="GFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEE
                     VR"
BASE COUNT         5609 a         3529 c         4314 g         5341 t
ORIGIN      
        1 tttgaaaaaa ataaaaaagg agattttatt atgaaattaa aaacacttgc tttaattagt
       61 ggtatcgtcg gtcttgtggg aggaatttta cttcttattg gtccttttgt cttgttggga
      121 acagcggtaa acacagttgc tacaactctt aatggaggag ctactgcagg ggctttttca
      181 ggtgttgcct tactcttgaa tgccttgaag attgcaaatc ttgttcttgg tatcattgct
      241 attgtttact ataaaggaga taatcgtgta ggtgcagctc cgtctgtact aatgattgtt
      301 tttggtggag ttagtctcat tccattctta ggatgggttg gggggattct tgttattatc
      361 ggaggatctc tattccttgc aacattgaag aaattcaaat cagaagaata aaaggtattt
      421 tagcatgaaa agaacaaaaa agtttatcgg tataggagta gctctattat ctctttttct
      481 tctagttgca tgtggaacat aaagttcaaa ggatacttca acaagtaatg atgagaagac
      541 agtagcaaca tccaatagtt caaaagaaac aatcactttc gatacaccgg ttgtaacaga
      601 cgatgtgatt gaatcaatac gcacttatgc agattatata gatctttata aaaatatttt
      661 tgatgattat tttactaaag ctgaggaagg tttcaaaggc acagctatgg aaaataatga
      721 ctcgtttact aaactaaaag agtcaactca aaaattattc gatgcgcaga aaaaaaggtt
      781 aaataatgaa gatagaatag aaacaactaa aaacaatgtg attgccaaac attgtcaaac
      841 agtcctttcc tttttggttt tgactagctt ttttgtgaaa aattgtgtaa aatagaatag
      901 ataaacgagg ggaaacctcg gaaaatttaa aggagaatcc atctaatggt aaaattggtt
      961 tttgctcgcc acggtgagtc tgaatggaac aaagctaacc ttttcactgg ttgggctgat
     1021 gttgatttgt ctgaaaaagg tacacaacaa gcgattgacg ctggtaaatt gatcaaagaa
     1081 gctggtatca aatttgacca agcttacact tcagtattga aacgtgctat caaaacaact
     1141 aacttggctc ttgaagcttc tgaccaattg tgggttccag ttgaaaaatc atggcgcttg
     1201 aacgaacgtc actacggtgg tttgactggt aaaaataaag ctgaagctgc tgaacaattt
     1261 ggtgatgagc aagttcacat ctggcgtcgt tcatacgatg tattgcctcc aaatatggac
     1321 cgtgatgatg agcactcagc tcacacagac cgtcgttatg cttcacttga cgactcagta
     1381 atcccagatg ctgaaaactt gaaagtgact ttggaacgtg ctcttccatt ctgggaagat
     1441 aaaatcgctc cagctcttaa agatggtaaa aacgtattcg taggagctca cggtaactca
     1501 atccgtgccc ttgtaaaaca catcaaaggt ttgtcagatg acgagatcat ggacgtggaa
     1561 atccctaact tcccaccatt ggtattcgaa ttcgacgaaa aattgaacgt cgtttctgaa
     1621 tactaccttg gaaaataaaa aattgtaagt ctagaattga tttctaggct ttttatgtta
     1681 gtatggaagt atgataagga ataaaaaaca agattatgta ctggcctaca agcaaccagt
     1741 ttcaaccact tacatgggtt gggaagaaga agctttaccg ataggcaatg gttctttagg
     1801 agcaaaagta tttggcctta taggggctga acggattcaa tttaatgaaa aaagtctctg
     1861 gtctggaggt ccacttcctg atagttcaga ttatcagggt ggaaatcttc aggatcagta
     1921 tgttttttta gctgagattc ggcaggcttt ggagaagaga gattacaatc tggctaagga
     1981 actggctgag cagcacctaa ttgggccaaa aacgagtcaa tatgggacct atctgtcttt
     2041 tggggatatt cacattgagt tcagccagca aggtacgact ttgtctcagg tgacggacta
     2101 tcagagacag ctgaatatta gtaaggcact tgtgacgact tcttatgtct ataagggaac
     2161 gcgatttgaa cgtgaagctt ttgcgagttt tccagatgat ctcttggttc agcgttttac
     2221 taaggaaggg gcggaaactc tagattttac tatagaacta tccttgacct gtgatttggc
     2281 ttctgatgga aagtatgagc aggaaaaatc tgattacaag gagtgtaagt tggatattac
     2341 tgattctcat atcttgatga agggaagagt taaggataat gatctgcggt ttgctagtta
     2401 tctagcttgg gaaacggatg gagatattag agtttggtca gatagggttc agatatcagg
     2461 agccagttat gccaatctct tcttggccgc taagacggat tttgcccaaa atcctgctag
     2521 caattatcgc aagaaactag atttagagca acaggtgata gacttggtgg acacagctaa
     2581 agaaaagggc tatacccaat tgaaatcaag gcatatcgag gactaccaag ccttattcca
     2641 gcgtgttcaa ttggatttgg aagctaatgt tgacgcattc actacagatg atttgttaaa
     2701 aaattataag ccacaagaag ggcaggcttt ggaggagctg ttcttccagt atggacggta
     2761 tttattgatt agttcgtcca gagactgccc agatgctcta ccagctaacc tacagggagt
     2821 ctggaatgcg gtcgacaatc ctccttggaa ttcggactat cacttaaatg tcaatctgca
     2881 gatgaattat tggccagcct atgttaccaa tctcctagag gcggtctttc cggtcatcaa
     2941 ctatgtagat gatttgcgtg tctatggtcg tctagcggct gtaaagtatg caggaatcgt
     3001 ctctcaaaaa ggtgaggaaa atggttggtt ggttcatact caagcgactc cctttggttg
     3061 gacggcacct ggttgggatt actattgggg ttggtcacca gctgccaatg cgtggatgat
     3121 gcaaactgtt tatgaagcct attcatttta tagggaccaa gactatctca gggagaaaat
     3181 ttatcccatg ttgagggaaa cggttcgttt ttggaatgcc tttttacata aggatcagca
     3241 ggcgcagcgt tgggtgtctt ctccgtctta ttccccagaa catgggccga tttcgattgg
     3301 caatacttat gaccaatctc tgatttggca gttatttcat gattttattc aggctgctca
     3361 ggaattggga ctggatgagg acttgttgac tgaggttaag gagaagtctg atttactaaa
     3421 tcctttgcaa atcactcaat ctggtcgaat cagggagtgg tatgaggagg aagagcagta
     3481 ttttcaaaat gagaaagtgg aggcccagca tcggcacgct tcccatctag tgggactcta
     3541 tcctggcaat ctctttagct acaagggaca agagtatatt gaagcggcac gtgctagcct
     3601 caatgatcgt ggagatggcg gcacaggctg gtccaaggct aataagatca atctctgggc
     3661 gcgtttggga gatggcaatc gagcccataa attattggca gagcagttaa aaacatccac
     3721 cttgccaaat ctttggtgta gccatcctcc ttttcagata gatggtaatt ttggtgctac
     3781 tagtggcatg gcagaaatgt tactccagtc tcatgcagct tatctggtac ctctagctgc
     3841 cctacctgat gcttggtcaa caggttctgt ttcaggctta atggcacgtg gacattttga
     3901 agtgagcatg agctgggaag ataaaaaact cttacagttg accattttat caaggagtgg
     3961 aggagatttg cgagtttctt atccagatat tgagaagagt gtgattaaaa tgaatcaaga
     4021 aaaaataaaa gcgaaatgca tggggaaaga ttgtatttcg gtggcaacag cagaaggtga
     4081 tcttgttcaa ttttattttt aagaagatgt tataaggcag taatttgaaa cttcctttta
     4141 ataaggattt aagaatatag gcagtttcca actagttgaa aaagcgttat aatgataata
     4201 ggaagtaata ctcaatgaaa atcaaagagc acaaactagg aagctagccg caggttgctc
     4261 aaaacagtgt tttgaggttg tagatggaag ctgacgtggt ttgaagagag attttcgagg
     4321 agtataattt gtttgataga gggtgggtct gatggcttat attgatatga aacactgtta
     4381 caagcgttat caggttgggg acacggagat tgtggccaat cgtgatgtga attttgagat
     4441 tgaaaagggg gagctggtta ttatccttgg tgcttcaggt gcaggcaagt caacagttct
     4501 taaccttctt gggggaatgg ataccaatga tgaaggggaa atctggattg atggtgttaa
     4561 tattgcggat tatagttccc accagcgcac caattaccgt agaaatgatg tggggtttgt
     4621 ttttcagttt tataatctag tttctaatct gacagctaag gaaaatgtgg aactggcttc
     4681 tgaaattgtg acagatgcct tgaatcctga tcaggtcttg acagatgtag gtctggctca
     4741 tcgtctcaat aactttccag cccagctttc tggaggggag caacagcgag tctccattgc
     4801 acgcgcggta gccaaaaatc ctaaaattct cctttgtgat gaaccgactg gagccttgga
     4861 ttatcagacg ggcaagcagg ttttgaaaat tctccaagac atgtctcgtc aaaagggagc
     4921 gacggtgatc atcgtgactc ataatggagc tttggcgccc attgctgatc gcgtgattca
     4981 tatgcacgat gccagtgtca aggatgtggt gctcaaccag catcctcagg atattgacag
     5041 tttggagtac tagcatgatc aagcgaaaaa cttattggaa ggacttaatt cagtccttca
     5101 cagactccaa ggggcgtttt ttatccatct tgatcctgat gatgttggga tctctagcct
     5161 tagtaggcct caaagtaacc agtcccaaca tggaggcgac agctaatgct tatttaacaa
     5221 ctgctcaaac cttggatttg gcagtcatgt ctaactatgg cttggatcaa gcagaccaag
     5281 aagaactaaa acagacggag ggcgcagagg tcgagtttgg ctatttgaca gatgtgacta
     5341 tggataatgg gcaggatgcc attcgactgt actccaaacc agagcgaatt tcaacctttc
     5401 agctaagaaa gggacgactt cctcagtcag acagggaaat cgctttggcc actcatttgc
     5461 aaggccaata cagcgtggga caggagatta gttttaaaga aaaagaagag ggtcattcct
     5521 ctttaaaaga ccatacttat accattactg gttttgtgga ttcggctgaa atcctctccc
     5581 agcgagatat gggctacgca ggaagtggaa gtgggactct gacagcctat ggggtgattt
     5641 tacctagtca atttgatcag aaagtctaca atatagctcg tttgaaatat caagatttag
     5701 caggtttaaa tgccttttca tcagcttatg aagaaaaatc caagcaacat caggaagacc
     5761 ttgaacaaac tttatcagat aatggcaagg tacgtctgca acttttgaaa aaagaaggac
     5821 aagagtctct agacaagggg caagagactc ttgacaaggc tcagactaat ttgcaggaag
     5881 gcaagcgtcg tttagcagct gctcaagctc gtatacaggc tcaagaaagt caactagcct
     5941 tgtttcctca agctcagaga gagcaggcta gtgctcaact tacccaagcc aagcaggaac
     6001 tgggcaagga agaggacaaa ctaaagcaag ctgaacaaaa tctagcccaa gaaaaggaaa
     6061 aattagaaaa acatcagcaa gtcttggatg atttggcgga gccaaggtat caggtttata
     6121 atcgtcagac catgccaggt ggtcagggct atcttatgta tagcaatgct tcatccagta
     6181 ttcgagcagt gggcaatatc tttcctgtgg tactttatgc cgtagcagcc atggtgacct
     6241 ttacgaccat gactcgcttt gtagacgaag agcgaactca tgcagggatt tttaaggcct
     6301 tgggttatcg tagtaaggat attatcgcca agtttctcct ttatggacta gtagctggga
     6361 ctgtcggaac ggctctaggt agtatacttg gtcattattt gctagccagt gtaatttcaa
     6421 gtgtcattac aaaaggcatg gtggtgggag aaacccagat tcagttctat tggacctata
     6481 gcttactagc tcttgtcttg agctggttgg cgagtgtgtt accagcctat ctggtggctt
     6541 ggagggaact tcatgacgaa gcagcccagc ttctacttcc taaacctcct gtcaaaggag
     6601 ctaaaatctt attggagcgt atcggtttta tctggcgtcg tctcagtttt actcataagg
     6661 taacagcccg caacatcttt cgttataagc agagaatgtt gatgacaatc tttggtgtgg
     6721 caggttctgt agctctgctc tttgcaggtt tgggaatcca atcttctgta gcaggagttc
     6781 cgtctaaaca gtttcaacaa atccaacagt atcagatgct tgtctctgaa aatcctagtg
     6841 cgaccaatca ggacaaggta gagctagcag aagtgttgaa agggcaggag atactagcct
     6901 accagaaaat ctattctaaa acgctagaca aggatttcaa gggcagggct ggtcttcaaa
     6961 acattactct tatgatgata gagaaggaag atttgactcc ctttatccat cttcaacatc
     7021 atcagcagga gctgacatta aaagatggca tcgttattac agctaaactc gcccagctgg
     7081 caggtgtcaa ggttgggcag actttagaaa ttgaaggtaa ggaactaaag gtcgctgcta
     7141 ttactgagaa ctacgttggt cactttattt atatgagtca ggctagctat gagcaacttt
     7201 acggacagct accccaagcc aacacttatc tggtctcatt aagggatacc agtgcaacta
     7261 gtatcgaaag tcaggcgggc ttgcttatga atcaatctgc ggtgtccagt gttgtccaaa
     7321 atgcttcagc cattcgactc ttcgactcta tcgctagctc actcaatcag accatgacca
     7381 tcttggtcat cgtatcggtt ctattagcta ttgtcatcct ttacaatctg accaatatca
     7441 acgtagctga gagaatccgt gaactctcca ctatcaaggt tctcggtttt cataataatg
     7501 aagtcaccct ctacatttac cgtgagacga ttgtgctgtc ccttgtggga atcgtacttg
     7561 gtctggtagc tggtttctat ttacaccaat ttttgattca aatgatttcg cctgcgacta
     7621 ttctctttta tccgcaggta ggctgggaag tctatgtaat cccagtggca gcagtaagca
     7681 tcattttgac cttgcttggt ttcttcgtca attattatct gagaaaggtt gacatgttag
     7741 aagccctgaa atctgtagag taaggtagtt atttttagct gattgaactt ctatttacta
     7801 atattcaaaa atcctccgtt tcaaagagca gggaactctt tgtgacagag gattttttct
     7861 atagggcttt agcagctgca attgcggctt cgaagtttgg ctcagaattg atattatcca
     7921 cgtattcaac gtagcgaatc gtattgtcag tatcgaggac aaagactgcg cgtgctaata
     7981 ggtgccattc gttgatcaag agggcataat cgcgtccgaa agaatggtca aagtagtctg
     8041 aaagcataat ggcattgtca aggccttcag caccgcacca acgtttttga gcaaaaggta
     8101 ggtccattga aacagtcaat acgaccgtgt tgtccagtcc agccaattct tcattaaaac
     8161 gacgtgtttg agttgagcag atgcctgtat cgatagaagg aacgacactc aagacttttt
     8221 tcttgccatc aaaatcagcc agagattttt tagaaagatc tgttgtagta agagaaaaat
     8281 caagcgcctt gtcgccgact tgtagttgtt tacctgtaaa gctcacagga tttccgagaa
     8341 aagttaccat aggatactcc aatctttttt cttccattgt atctgaaaca gtcagaattt
     8401 tccaatgatt tgaccggaaa tgtgtgcata gaaaaaacgc cagctcacat gagaatgacg
     8461 tttttcagag gcttattttg ccaatccttc agcaatcttg tcaaggttgt atttcatcat
     8521 gctgtagtag ctgtcgcctt ctttaccttg ttctgcgata gagtcagtaa agatttgtgc
     8581 gtagattggg atgtttgtgt cttgagaaac agttttcatt ggacggtcat ccacacttga
     8641 ttctacaaag agtgatggaa cttttgtttg gcgaagtttt tcaaccaagg tcttgatttg
     8701 ttcaggagtt ccttcttctt cagtattgat ttcccagatg taggcacttg ggacaccata
     8761 ggctttagag aagtatttga atgctccttc gctggttaca atgagtttct tttcagcagg
     8821 gatcttatta aatttatcct tactttcttt atcaagtttg tctaacttat cagtatattc
     8881 tttgagattt ttttcataga attctttatt gttagggtct ttggcgctca attgtttggc
     8941 gatattttta gcaaaaataa taccgttttc aaggttaagc caagcgtgtg ggtcttcttt
     9001 tcctttttca ttttgacctt caaggtagat aacatcaacg ccgtcgctga ctgcgaagta
     9061 gtctttgttt tcagttttct tggcattttc taccaatttt gtaaaccaag cattgccacc
     9121 tgtttcaagg ttgataccgt tatagaaaat caaatcagcc tcagaagttt tcttaacgtc
     9181 ttcaggaagt ggttcgtatt cgtgtgggtc ttgcccaatc ggaacgatac tatgaaggtc
     9241 aattttgtca ccagcaatat ttttagtaat atcagcgatg attgagtttg tagcaacaac
     9301 ttttagtttt tgaccagaag ttgtatcttt ttttccgcta gcacatgcta caagaatgat
     9361 tgcagaaaga aagagaacga gtaatgtacc taattttttc attagatcct ccaatttatt
     9421 agggctttgc cccttatttt aacaaatgtt tatttttcag tttcaaatat cgttgtttgg
     9481 gagcgataaa gaagctaatg agaaagaaac tagcagctgt aagcacgata ctagaacctg
     9541 ccgcaacatt aaaactatag ccaataaaga gtcccaaaac tgaagcagta gctccgaagg
     9601 ttgaggaaag gaaaatcatg cttttcagac tattagcata cagataagca gttgcagctg
     9661 gggtaatcag catggctaca atcaggatag ttccgacact ttgcatggct gtcacagaca
     9721 cgagagtcag gagtaccatg agaaggtagt gatagaaatt gacaggcatt cccatggctt
     9781 tagccaagag ttcatcaaag gaagttatca agagttgctt gaagaaaatc cagattaaca
     9841 agaggatggc tgcccccaca cccatagtaa taaacatatc cgtatcttgg acggccagaa
     9901 tattaccaaa aaggatatgg aaaaggtcag ttgaactttt agcgacacca atcaagatga
     9961 taccgagggc taagaaagaa gaaaaggtaa tgccgatggc ggtatcgctt ttgataatcg
    10021 agtttccttt gatgtaggta atgatgatgg cagctagcaa tccaaagaca atggctccga
    10081 taaagaagtc aaggcccaag atgaaggaga gggctacacc tggtaagaca gcatgtgaaa
    10141 tggcatctcc catgagtgac atcccgcgta gaataatgaa acatcccacg gctccagcta
    10201 caatcccgac gacaatagct gttatcaagg cattttgtag gaaatggaat ttttgcaatc
    10261 catcgataaa ttctgcaatc ataggtcacc tccattgaaa aagagttgat taccgtaagc
    10321 ttcttttaga ttggtttcgg taaaagtttc ttttgttgga ccaaaggcaa tcacttctcg
    10381 attgacaagt aagacttgat cgaagtagtg gggaatcttg ctgaggtcgt ggtgaacgat
    10441 gagaaccgtc ttcccagctt ttttcaaatc tctcagcgta ttcatgatga tttcctcact
    10501 gacagagtca atcccagcaa agggttcatc caagaggata tagtcggctt cctgcaccaa
    10561 acatctggca atcaagaccc gctggaattg acctccagac agttgactaa tttgacgttc
    10621 agcgtagtca gctaggccga cgatttcaag ggcctcttgc actttcttcc aatgtttagc
    10681 ctttaaactt cgaaagagag gaatagaggg aaatagtcct aacgagacgc attccttgac
    10741 cttgatggga aagttgtagt cgatattgat tttttgttcg acataggcaa ttcggtgtaa
    10801 ggatttttta acttccttgt catcgagaaa tgcctgacct tgatgtggga taattcccag
    10861 catacctttt aatagtgttg atttcccagc gccgtttgga ccaatgatgc cggtaattgt
    10921 tggtccatgg agcactagtg aaatatcctt aagtgccaac gtttctttgt aggagacact
    10981 gaggttttcg atacgtatca taaacttata ttcctcctgt ctcttaatat acattaaaaa
    11041 aaaaattaag tcaagttaat ttttgaaaaa attaaaataa taactgaaaa atagattcta
    11101 aagataactt tcaggataaa tttctaaatt ataaaacgca tagtatgcat agtatcaagt
    11161 gtaaaaaact tggaattatg cgttttatca tggaaagatt ctttataata gctaaaaaat
    11221 aatagttaaa tggttactaa ttgcattccc tagtgatttt tgttaagata aaggcaaata
    11281 caaatgaaag cgagaacaag atgacacgtt atcaagatga tttttatgat gctatcaatg
    11341 gagaatggca acagacagct gaaatcccag cagataagtc tcaaacagga ggttttgttg
    11401 atttagacca ggaaattgaa gacttgatgc tggcgacaac agacaagtgg ttagcaggtg
    11461 aagaagtgcc tgaggatgct atcttggaaa actttgtcaa ataccaccgc ctagttcgtg
    11521 attttgacaa gagagaagct gacggtatca cacctatctt atcactcctt aaagaatttc
    11581 aagaattgga gacttttgcg gattttatag ctaaactagc agagtttgag cttgcaggaa
    11641 aaccaaactt ccttcctttt ggtgtatcgc cagactttat ggatgctaga atcaatgttc
    11701 tatgggctag cgctccaagc acaatcttgc cagatacgac ctactatgca gaagaacatc
    11761 ctcagcgcga agagctcttg actctttgga aagaaagcag cgcaaatctc ctcaaggctt
    11821 atgatttctc tgatgaagaa attgaagact tgctagaaaa aagacttgaa ttggaccgcc
    11881 gagttgcggc agtggtgctc tctaatgaag aaagttcaga atatgctaaa ctctatcatc
    11941 catattctta cgaagatttc aagaaattcg cgcctgccct acctttggat gacttcttca
    12001 aagcagttat tgggcaatta ccagacaagg ttattgtaga cgaggaacgt ttctggcaag
    12061 cagcagagca attctacagt gaggaagcct ggtctctcct taaagcaacc ttgattttga
    12121 gtgttgtcaa tctttcaacc agctatttaa cagaggatat ccgtgttttg tctggtgcct
    12181 acagccgtgc cctttctgga gttccagagg caaaagataa ggtcaaagca gcttatcatc
    12241 tagcacaaga acctttcaag caagccctgg gtctttggta cgcccgtgag aagttctctc
    12301 cagaagccaa ggcggatgtg gagaaaaaag tggcaaccat gattgatgtc tataaggagc
    12361 gtctgcttaa gaatgactgg ctcactccag aaacctgtaa acaggctatc gtgaagctca
    12421 atgtgatcaa accttatatt ggctatccag aagaattgcc tgcacgttac aaggataagg
    12481 tagtgaatga aactgccagt ctttttgaga atgctctagc ctttgcgcgt gtggaaatca
    12541 agcacagttg gagtaagtgg aaccagcctg tagattataa ggaatggggc atgcctgctc
    12601 atatggtcaa tgcctactac aatcctcaga agaacctgat tgtctttcca gcggccattt
    12661 tacaggcgcc tttctatgac ttgcatcagt catcttctgc taactacggt ggtattgggg
    12721 cagtgattgc ccatgaaatt tcccacgcct ttgatactaa cggggcttcc tttgacgaaa
    12781 atggtagcct caaggattgg tggacagaga gcgactatgc tgccttcaag gagaaaacac
    12841 aaaaagtcat tgaccaattt gatggacagg attcttatgg agcaaccatt aacggtaaat
    12901 tgactgtatc agaaaacgtg gctgacttgg gaggaatcgc agcagcgctt gaagcagcta
    12961 agagagaagc agacttctca gcagaagagt tcttctacaa cttcggtcgc atctggcgca
    13021 tgaaaggtcg accagaattt atgaaacttt tggctagcgt cgatgtgcac gcaccagcca
    13081 aactccgtgt caatgtgcaa gtaccaaact tcgacgattt ctttacaacc tatgatgtca
    13141 aagaaggaga cggaatgtgg cgttcaccag aggagcgcgt gattatttgg taatacaaaa
    13201 aatctagtca tcagaaaaaa ggcatccaat cacgtgtgaa aaccttgata ggatgccttt
    13261 tgtaatagaa agatttgctg gatagtttac ttaaatttcg attgtcatca tcttgctaga
    13321 aaaagggatt gaaggccttt tcgtgagcga tagtagtagc tggaccatgt cctggataga
    13381 catcgtagtt tggtagggtg aagagttggg tctggatact atgaaggagt tgctccatgc
    13441 taccagtcgg aaggtcggtc cgtccgatag tttcgcggaa tagagcatct cccgtcaaga
    13501 ctaggtgagc atcaggaaag actagggaaa caccaccgat agagtgccct ggggtcggta
    13561 gaaccttaaa acgaaattcc tctagttggt attcttcgtg aaagacaaag gtgtgttctg
    13621 caggttttgt gaccacatct gccatatcat cgtggcgagg gagaccggag agattatcga
    13681 caggagtgta gagccagctg gcttcgctct ctgcgatata gacaggagga ttgccaaacg
    13741 tctcgcgaac caagtccaga ctcatgatat ggtcataatg ggcgtgggtc aagagaatag
    13801 cacagatcgg tttgttgatc ttctcgattg tctgacgaat ggcttcccaa tgactaccag
    13861 gatcgacgac gatgaggtgc ttttcgcctt ctagataata ggtattttca taggcaacag
    13921 gattcacggt tttatggatt ttcatactag ctccaatctc aaagaatgta ctaatactct
    13981 tcgaaaatct cttcaaaccg cgtcaacttt atctgcaatc tcaaagcagt gctttgaaca
    14041 gcctgcggct agctttctag tttgctcttt gattttcatt gagtataaat taagtatagc
    14101 acagttaggg agaataggta aggatttaaa atgaacttaa aaaccagcat gactagatga
    14161 agacaggctg gttatcaatt tatcaatatt ggaaaggtgt caattgccct tcataggttg
    14221 cataaactcc gtcattagct tgtttgatag acgtgatgtt tagagtgcta ccgtagttgg
    14281 cttctcattt gtcgctatat tcactataag ttatcctact gggcaggttc tggtcgttag
    14341 catagtattg aacgactgtt ttttatagtt ttgctggctg aataggaaca ggcagcaaac
    14401 tcctaacatt aggagagcaa agatagaaat agcagttttt ttcataggta ttctccttat
    14461 aaactaaagg atagaagaaa gtatcttgag gatttttata ctcttcaaaa atctcttcaa
    14521 accgcgtcaa cgtcgccttg ccgtatatat gtgactgact ttgtcagtct tatctacaac
    14581 ctcaaagcag tgctttgaac agcctgcggc tagtttccta gtttgctctt ggattttcat
    14641 tgagtattaa ttccaaaatc cagtcaaggt accagtgtat gtgatacgag ttccaccgtt
    14701 tatctttcgt atcttcaatg gaattgggga agtcagaaag tctttgggtt acaatatggt
    14761 ttccgttctt ctatctctcc ttctatcacc taagtttaaa atctaacttc gtagtatatt
    14821 atggaccaat agttcagaat tatcaagcaa ttttgaatta tttttttctt attctgttcc
    14881 aatctagttt tcatattctt caaaaagtga taaaatggta ggaagaattg gagagtagag
    14941 atgccgaaag aagtgaattt aacaggcgaa gaagttgtcg ctttaaccaa agaatattta
    15001 acggaagagg atgttcattt tgtccataag gccttggtct atgctgttga atgccacagt
    15061 ggtcaatatc gcaaatcagg cgagccttat atcattcacc ctatccaagt ggcaggtatt
    15121 ttagctaagc taaagctgga tgctgtaaca gtagcttgtg gattcttgca tgatgtggtg
    15181 gaagatacag atgcgacctt ggacgatttg gaaagagagt ttggtcctga tgtgcgggtg
    15241 attgttgacg gagttaccaa gcttggcaag gtcgagtaca aatcgatcga ggagcaatta
    15301 gcggaaaatc atcgcaagat gctcatggcc atgtctgagg acatccgcgt tattttggtc
    15361 aaactgtctg accgcttgca caatatgcgg accctgaaac atcttcgaaa agacaagcag
    15421 gagcgtattt ccaaagaaac catggaaatc tatgccccac ttgcccatcg tttggggatt
    15481 tccagtgtca aatgggaatt agaagacttg tctttccgtt atctcaatcc aacggagttt
    15541 tacaagatta cccatatgat gaaggaaaag cgcagggagc gtgaggcctt ggtggatgag
    15601 gtagtcacaa aattagagga gtatacgaca gaacgtcact tgaaagggaa gatttatggt
    15661 cgtcccaagc atatttactc aattttccgc aaaatgcagg acaagagaaa acggtttgag
    15721 gaaatctatg atctgattgc tattcgttgt attttagata cccaaagtga tgtttatgcc
    15781 atgcttggtt acgtgcatga attttggaaa ccgatgccag gtcgcttcaa agactatatt
    15841 gccaaccgca aggccaatgg ttatcagtct atccatacga ctgtttatgg accaaaaggg
    15901 ccgattgaat tccagattcg aaccaaggaa atgcacgagg tggctgagta cggggttgcg
    15961 gctcactggg cttataagaa aggtataaag gggcaagtta acagcaagga atcagctatt
    16021 ggaatgaact ggatcaagga gatgatggag ctccaagacc aggctgatga tgctaaggaa
    16081 tttgtggact ctgttaagga aaactatctg gctgaggaga tttacgtttt taccccagat
    16141 ggagctgtcc gttcccttcc caaagattca ggaccgattg attttgccta cgaaatccat
    16201 accaaggtcg gtgaaaaagc aactggtgcc aaggtcaatg gccgcatggt tccactgaca
    16261 accaagttaa agacagggga tcaggttgaa attatcgcca acccgaactc ctttggacct
    16321 agccgtgact ggctcaatat ggtcaagact agcaaggcgc gcaataagat tcgccagttc
    16381 tttaaaaacc aagataagga attgtctgtc aacaagggtc gtgagatgct gatggctcag
    16441 ttccaagaaa atggctatgt ggcaaataaa tttatggaca agcgccacat ggatcaagtt
    16501 ctgcaaaaga ccagttacaa gacagaagac tccctctttg cggccattgg ttttggggaa
    16561 atcggtgcga ttaccgtctt taaccgtctg actgaaaagg aacgccgtga ggaagagcgt
    16621 gccaaggcca aggctgaggc agaggagctt gtcaaaggtg gcgaggtcaa ggttgaaaat
    16681 aaagaaaccc tcaaggtcaa gcatgagggg ggagtggtta ttgaaggtgc ttctggtctc
    16741 ctagtgcgga ttgctaagtg ttgtaacccc gtgcctggtg acgatattgt tggctacatt
    16801 accaagggtc gtggtgtggc tattcaccgt gtggactgta tgaacctgcg tgcccaagaa
    16861 aactacgagc aacgtctcct tgatgtggaa tgggaagacc agtactctag ctcaaataag
    16921 gagtatatgg cccatatcga tatctacggt ctcaaccgta caggactgtt gaacgatgta
    16981 ctgcaagttc tttcaaatac aaccaagaat atttcaacgg tcaatgccca accaaccaag
    17041 gatatgaagt ttgctaatat ccatgtgtcc ttcggtattg ccaacctctc tacactgacc
    17101 acggttgtcg ataaaattaa gagtgtgcca gaagtttact ctgtcaaacg gaccaacggc
    17161 tagttgtcct agctcttact agaaaggcta ttatgaaaat cattatccaa cgggttaaaa
    17221 aagcccaagt gagtatagaa ggccagattc agggaaaaat caatcaggga cttttattgc
    17281 tggttggtgt tggaccagag gaccaagagg aagatttgga ctatgctgtg agaaaactgg
    17341 tcaatatgcg gattttttca gacgcagaag gcaagatgaa cctgtctgtc aaagatattg
    17401 aaggagaaat cctctctatt tctcagttta ccctctttgc ggatactaag aaaggcaatc
    17461 gtccagcctt tacaggggca gctaaacctg atatggcatc agacttctat gatgctttca
    17521 atcaaaaatt agcgcaagaa gtgcccgttc agacaggcat ctttggagca gatatgcagg
    17581 ttgagctggt taataacgga cctgttacca ttatcctaga cactaaaaag agataagaaa
    17641 gaccaagccc agtcggcttg gtctttctca tcgatcataa aatactccaa aaagaaatcg
    17701 gttcttgata tgcttggggg aagtctcttt tcaggctttg gcagatgcga taggaaggga
    17761 tgagatgtcc taggatgagg agagttccct gaaggaaaag tggaatatca cttaaagaca
    17821 ccaaggagag aatcatcagg ctatcccata ggagaatttg taaagcaaaa cgccagaatc
    17881 tcggtctaat ctgggaatag agttgactaa agtggtcaca aagttggttt gaaagatagg
    17941 tcaatagcac aggatgaaag aaaatgagcc aaccgctata aatcaaaatc cagtcccaag
    18001 taagcccccc tttttaaatg tcaaaaatgc cagcatgatt ccatcaatga caaggagttg
    18061 aatggttttg atgaagatga tttttttcat gctaaaacct ccttttcctt agagacactc
    18121 ccataaagag atgtgtttgt cgtaaaagtc tggatagttt tctcctaggt agtcagctgt
    18181 catataataa aaagtagaat ggcaagcagt gataattggc ataagagatg aaaaacgctg
    18241 agaactaatg aaggctaaga gaacaaaaaa tagaaaatca atggtgagaa cccctaaata
    18301 gtagaagcga atctttttat tgatttgagg ataaatctca gcgaattgat gtttgagtcg
    18361 gacgaccaag aaaaatagca ggagtccttg agcaaggaag aaaattaaac cagagagcaa
    18421 gagccactca aaagatgtct cgagtctagt gataaaaaat gcaaatagta gcaaatcaat
    18481 agctgcaatt gctataaaat ggattctata gagttttttc atgacaagac ctccctcttt
    18541 tatctaactt cattctactc caaaagaatg ggagttacaa ctaaaatgat aaaaatagca
    18601 gaagggagat tctcttaagt tggctagtat tctttatttg agtttccttc tattatctaa
    18661 cttcttcatc attccagaca aataaagctc cgattgcatt gaggatataa aagatgtatt
    18721 taccgatatt ggcgaagttt ccttgaatac cagcttttgt cagctgaacg aaattgtaaa
    18781 tcaaccaaaa gcc
//
LOCUS       DAVKHZ010000040        18652 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000040 DAVKHZ010000000
VERSION     DAVKHZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.040"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..863)
                     /gene="lytB"
                     /locus_tag="VWW99_001259"
     CDS             complement(<1..863)
                     /gene="lytB"
                     /locus_tag="VWW99_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU9539101.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGSFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGG"
     gene            complement(905..1843)
                     /locus_tag="VWW99_001260"
     CDS             complement(905..1843)
                     /locus_tag="VWW99_001260"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9539102.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1854..2654)
                     /locus_tag="VWW99_001261"
     CDS             complement(1854..2654)
                     /locus_tag="VWW99_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9539103.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2904..3284)
                     /locus_tag="VWW99_001262"
     CDS             complement(2904..3284)
                     /locus_tag="VWW99_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9539104.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3342..3701)
                     /gene="rplT"
                     /locus_tag="VWW99_001263"
     CDS             complement(3342..3701)
                     /gene="rplT"
                     /locus_tag="VWW99_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9539105.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3753..3953)
                     /gene="rpmI"
                     /locus_tag="VWW99_001264"
     CDS             complement(3753..3953)
                     /gene="rpmI"
                     /locus_tag="VWW99_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9539106.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3986..4516)
                     /gene="infC"
                     /locus_tag="VWW99_001265"
     CDS             complement(3986..4516)
                     /gene="infC"
                     /locus_tag="VWW99_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9539107.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4532..4663)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4823..5946)
                     /locus_tag="VWW99_001266"
                     /pseudo
     CDS             complement(4823..5946)
                     /locus_tag="VWW99_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5950..6537)
                     /locus_tag="VWW99_001267"
     CDS             complement(5950..6537)
                     /locus_tag="VWW99_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539108.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            complement(6570..6758)
                     /locus_tag="VWW99_001268"
     CDS             complement(6570..6758)
                     /locus_tag="VWW99_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539109.1"
                     /translation="MTATKSYKYDWNTVLEYSINYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYSGGY"
     gene            complement(6937..8927)
                     /locus_tag="VWW99_001269"
                     /pseudo
     CDS             complement(6937..8927)
                     /locus_tag="VWW99_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family competence protein"
     gene            complement(8911..9552)
                     /locus_tag="VWW99_001270"
     CDS             complement(8911..9552)
                     /locus_tag="VWW99_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9539110.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTEKEVNKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQKAGG
                     LTEQADSKSLNLAQKVSDEALVYVPTKGEESASQQAGSGAPSSTSKDKKVNLNKASLE
                     ELKQVKGLGGKRAQDIIDHRETNGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            complement(9620..10189)
                     /locus_tag="VWW99_001271"
     CDS             complement(9620..10189)
                     /locus_tag="VWW99_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539111.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     IFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10366..10791
                     /locus_tag="VWW99_001272"
     CDS             10366..10791
                     /locus_tag="VWW99_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9539112.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            10792..11205
                     /locus_tag="VWW99_001273"
     CDS             10792..11205
                     /locus_tag="VWW99_001273"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9539113.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            11151..11477
                     /locus_tag="VWW99_001274"
     CDS             11151..11477
                     /locus_tag="VWW99_001274"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539114.1"
                     /translation="MVDVAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(11844..12103)
                     /locus_tag="VWW99_001275"
                     /pseudo
     CDS             complement(11844..12103)
                     /locus_tag="VWW99_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(12106..12606)
                     /locus_tag="VWW99_001276"
     CDS             complement(12106..12606)
                     /locus_tag="VWW99_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539115.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            12642..12821
                     /locus_tag="VWW99_001277"
     CDS             12642..12821
                     /locus_tag="VWW99_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539116.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(12799..13767)
                     /locus_tag="VWW99_001278"
     CDS             complement(12799..13767)
                     /locus_tag="VWW99_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9539117.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(13853..14068)
                     /locus_tag="VWW99_001279"
     CDS             complement(13853..14068)
                     /locus_tag="VWW99_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9539118.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(14077..14931)
                     /gene="cvfB"
                     /locus_tag="VWW99_001280"
     CDS             complement(14077..14931)
                     /gene="cvfB"
                     /locus_tag="VWW99_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9539119.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(14991..15548)
                     /gene="frr"
                     /locus_tag="VWW99_001281"
     CDS             complement(14991..15548)
                     /gene="frr"
                     /locus_tag="VWW99_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9539120.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(15557..16294)
                     /locus_tag="VWW99_001282"
     CDS             complement(15557..16294)
                     /locus_tag="VWW99_001282"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9539121.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(16378..17712)
                     /gene="trmFO"
                     /locus_tag="VWW99_001283"
     CDS             complement(16378..17712)
                     /gene="trmFO"
                     /locus_tag="VWW99_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9539122.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(17957..18211)
                     /locus_tag="VWW99_001284"
     CDS             complement(17957..18211)
                     /locus_tag="VWW99_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9539123.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            18206..18652
                     /locus_tag="VWW99_001285"
                     /pseudo
     CDS             18206..18652
                     /locus_tag="VWW99_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5375 a         4214 c         3460 g         5603 t
ORIGIN      
        1 ccaccggatt tgaagtagta ccaagcttga ctatgagaat cgtagaccca ttctttttca
       61 gctattttcc catcaaactt gaggtaaaac caagattcct tatcccaaat ccattcattg
      121 gctgccatgt agccaccgga ttttagataa taatagtgac cattctcgaa aatccattct
      181 ttatcagcat agtttccatt ttctttgatg taaaaccaag attggtattg tttgtcaaaa
      241 agccaacctt gctgtacttt ggcaccacta gcattcacat aagcttgatt aatccactga
      301 cttgtcagta gataaccacc ggatttgaaa taatagtcct tccctttaat ttggagccat
      361 tctttctctg cgtgctgtcc atctgctttg atataaaacc aagcatcgta ttgagaatca
      421 tagacccagt cttctattac tttggcacct gttgcaccaa cataggaagt tcctacccaa
      481 gcatttcttt tcatctttcc atcttggtca agataataaa aggatccctt gtcttctacc
      541 cattctgatt tggccatata gccaccagat ttgaggtaaa aatagtcgtc accttgcttt
      601 agccattcat tttcagcata gttagcatct gcttttatat agaaccaaga ttgataatga
      661 gtatcaaaaa cccactcatt cgctgcttga ctgccatctt ctttcagata ctgttttcct
      721 tgccaagtac catcactagc cattgcaccc tctggactag ctaagaaaaa tagcagagct
      781 aaaaaaataa atcttacttt cttcattaac cttcttcctc tgttcttatt tattttattg
      841 taccaaaatt ctcctaaatt catattacaa actaataaaa aatcaagaac agattgattg
      901 cagtttacct cagagactct tttacttcct gacggagttc ttccagactg ctaataccgt
      961 atttatccat gacttttggt aaattttcga tgatgtcagg gcaggcataa ggattggtaa
     1021 agttagctgt tccaactccg atagcagatg ccccagccag atacatttct agggcagctt
     1081 cagccgaatc cactcccccc attccaatga taggcaggtc tgttgtttgg gctacttggc
     1141 ggatgagttt gagggctact ggaaagactg ctggacctga cattccacct gttccattgg
     1201 ccaagattgg ttttctggtt ttgaggtcaa agcgcattcc aaccagagta ttgatcatgg
     1261 tcaagccact tgctcccgca tcttctgcag cttttgcgac agtaacgata tcggtcacac
     1321 tcggggttaa tttgacataa actggcactt ctgaggcttc cacagctgct ttcaccacat
     1381 cataagccaa atctggatct tgaccaatca aaagtccatg attacagtgg tcaacattgg
     1441 gacaagaaat attgagctcg atagctttta cattagttac cttggaaatc ccatgagaaa
     1501 cagctgcata ctcttgtttt gaaaaaccag ccacattggc aataatagga agatttggat
     1561 attctctttc cagccaaggt agcttttcag ccaaaacaac ctctaaacca ggattttgca
     1621 agccaattgc attgagcata ccagcaggcg tctctgccac tcttggagtt ggatttccaa
     1681 aacgtggttc aagggttgtc gccttgatca taatagaacc taaaaggtct aaatcatagt
     1741 acttggcata ctcttgtcca aagccaaaac agcctgatgc tggaataatc ggatttttca
     1801 aatccaaacc aggtagagaa acttgtaatc gatttgtagt cataattttc tccttataat
     1861 acaactgttc ctgtgcggaa aacaggacca tcttcacaga cgcgttggct gaccgtctcg
     1921 ctttctggta cttttagaac acaggcatag caagctccca tcccacaagc catacgagat
     1981 tccagagata aataggctct tgggtgatca tcaaaggttt gattgatata cttcatcatt
     2041 cctggagccc cacacgagta aacagcatca aactgactgt ctaaatcatt gataacaacg
     2101 gaaacatttc ccttgatgcc ataagaacca tcatctgtcg ttacaaagac ctgaccatac
     2161 tgagccaatt ccgttttcaa aataacagca tccttattag caaaaccgag gactgtcact
     2221 actttcactc cacgttcatg caattccttg gccacctcaa gcaagggtgg aacaccaatc
     2281 ccaccaccaa caaggagaac ctgattctgc tcatcaaggt cagacaagtc aaaaccattt
     2341 ccctgaggcc ccatcacatc aagagtgtct ccctgactta aggttgaaaa aattgcagtc
     2401 ccagctccct caatccgata aatgaggtga cactgcttgt ttgccttgtc aatagacgaa
     2461 attgaaatag gacgacgtaa gagatgggca tcgtctggta cacgcagatg aagaaattgg
     2521 cctgctcgca tggcttcaac catttctcct tctaggacta attcaaagat tgctggcgcg
     2581 atttcctctt gtgcaaccac cttcatggtt tccaaccgaa tgacacccaa acgcttctta
     2641 catgtgagat tcataacttt tcctccttaa attttaaggg gaccagataa agaaaagacc
     2701 ttgctagtgc aaggcctcag ttaaaatcgt acaacacaag agagcacact caaaaggtct
     2761 cctctagaaa attgcactat tcttttatct gacaccttgt gagtctctct ggactcccct
     2821 taaaggttga atttttatta gtatactctt tcagctaaaa aaagtcaagt agaaaacgaa
     2881 tattctactt aacttgcacg aaattatttt tcacgaatga cttcgacctt atatccatca
     2941 gggtctttaa caaagtaata gtttggtgta gttcctggta gaccatttgg ctcagtcact
     3001 tcatagcctt ttgcactgtg ttcttgatga agtgcctcaa gatcaggtgt actgagggcg
     3061 atatgggcaa acccatcacc aaccacatac ggaccgtgat cgtagttata agtcaactcc
     3121 aactcataat catcaccctc aagacctaga tagacaatcg tgaaggcatg gtctggaaaa
     3181 tctctgcgac gcaattcttt aaaaccaaaa gcatcttgat aaaatgcgat tgatttttca
     3241 agattttcta ctcgtaagca agtgtgtagc atttttgaag ccatatcttt ctcctttatt
     3301 tttaaaaaga ctggacaatc ctgttccagt ctcatcagtt gttatttacc aagttttgct
     3361 ttagctgcat ctgcaagagc tgtgaaagct actgcatcgt taacagccaa gtcagcaagc
     3421 attttacggt taacttcgat ctcagccaat ttcaaaccat gcatcaattg tgagtatgaa
     3481 agtccgttca tacgagctgc cgcattgata cgagtaatcc acaatttgcg gaagtcacgt
     3541 tttttctgac gacggtcacg gtatgcatag tagtaagagt tcattacttg ttcttttgca
     3601 gtacggaaca agatgtgttt agctccatag taaccttttg ctaatttaag aatacgttta
     3661 cgacgtttgc gtgatacaac gccaccttta acacgtgcca tattttattt cctccaaata
     3721 tttcctagaa ttgtttactt acagtcaagc tattatttca agcgagtaag cattgctttg
     3781 atacgtttgt aatctcctga atgcaccata gatgctttac gaagatgacg acgttgtttc
     3841 ttggtttttc cgtggaaacg gtgagaagtg taagcacgga aacgtttaag tccaccagaa
     3901 cctgtacgtt tgaaacgttt agctgatgcg cggtgtgttt tttgttttgg catgattttt
     3961 tctcctttat ttaactttct gacaattatt ttttgtcagt cgctggcgcc aattgcatga
     4021 acatttgacg tccatccatt ttagcacgtt gttcgatgat ggcaatatct tgagttgctt
     4081 cagcaaactc ggctaaaact tttgcaccaa tctctttatg ggtaatcata cgacccttaa
     4141 agcgaataga taccttaact ttatttcctt tttcaaggaa cttacgtgca ttacgaagtt
     4201 ttgtatcaaa gtcacccttg tcaatagttg gacttagacg aacttctttc acagtaacaa
     4261 cgctttgttt tttacgttgt tctttttgct tcttctggta ctcaaatttg aacttaccgt
     4321 agtccataat ttttgcaaca ggcggtttgg cttggggttg aatcaatact aggtcaacat
     4381 tagcgttatc agccaaagct tgcgcttcac tgagtggctt gatgcctagc tgttctccct
     4441 caagaccaat caagcgaact tcacgtacac gaatctcatc attgatgaat aagtcttgct
     4501 ttgctatggt tttcacctct tttgttttat tagagaaaaa caatagcgga ctcgtaaata
     4561 tacaagcccg cacgttatga tagcgtttct tcgaaacttt tcatccgtag ggccaggcaa
     4621 cttaatgtca caaggcgaga agctctcact tctgcttttc tcaactttta tatgatacca
     4681 agttttctag ctcttgtcaa gataaaaagt tattttttga aaagtttgtg tttatatttt
     4741 ggtttggttt tcgaatcaaa aagagagaac caagttgatt ctctccctat gttagttttt
     4801 ctttagcttc cctataggaa aactaaactt taaaaacttc ctttttacgt gaacttcccc
     4861 atctttcgat aaatagaatc cccactaaca aaaggataat caggcaagga agtaagacca
     4921 tccccatgct ccaatttaga tcgacattat caatctgctt agataatctt ccagataaaa
     4981 tctccactga acgcaagtaa gtaaagggaa tcagatgtgc aatcctttga agaggacagg
     5041 cattttttgc ttgaaaaagt aagcaatcaa gtacacaact tccacaatga cgataaaggc
     5101 taagaaagct aagagcaagc caggaaataa tacatcttgt attttcccaa tagttacttc
     5161 ttgattcact aagctataaa ttgggtaggg ataatctaac tgtccaaaac cacttatcag
     5221 acttcccact agaaaagaaa agccacagat tccgataaac agcacagtta catatcccac
     5281 tccaactcca agagaggata ttgcaaatgt cacttttgaa acaggatata agtgagctgt
     5341 gtccagatga ttttgatatc tttctgcaaa tagttgtgtt agcataaaaa taattgcaac
     5401 cacaaacaag cttgggatga taacctctaa aatccagaca atctgatcaa tcccgtgggt
     5461 cggaaactcc aaagtatgtg cttttatgtt caagggatac agggcttggt aaatcttccg
     5521 ttcgcggtca acccccattt ttaagccaga gctagcagtc gggtcatttg atacaaattc
     5581 ataattcttc tcttcatctt gccactgcag atagtaggcc tctttccagc gcccttcttt
     5641 taataaagtc agaatttctg tctttcgcgt caaaagattt ttttgcacgt ctaaattatt
     5701 tttagcaaac tggtattcct ccgagctggt atcagacatt tgggagagtt tctcttcatt
     5761 ttcattgata gccctctcgt tggctgcaat gcgactttcc aacctgctct ccaagctgtg
     5821 tgagtttgca gtctgactat ttaaataaaa ggtaacaccg agtacagatg caaataaaag
     5881 taagataatc cagtttaaac gacttttgaa aacttttttc aataaaaata gactaacatc
     5941 tttcataaac taaacctctt ctatctgccc ctgatgaatg gttactactc tatcgcagat
     6001 atcaaccaac tcttccttat agtgggaact taaaagaacc agctgttctt gtctatcgat
     6061 ttgtgctagc ctatcaaaaa acttctgtcg ataatactcg tctaagccat ttgtaatctc
     6121 atccatgagc cagcatttgg cctgactgag aaaatacata gcaatcacca agcgttgctt
     6181 catgcctaag gaatacttgc ggatgggaag actgatatag tcagccattt cccagtaggc
     6241 gatttcatct ctcaagttta ggtctgactt ccagatgttt tttatgagac gaaggtagtc
     6301 catcccactt aagtttccat ccagccattc aacgctctca taataaaaca aagaaggagg
     6361 aactgcgata tttccactac ttatgggaat taaattgcta atggcacgga acagggtcgt
     6421 ctttccagag ccattgatag caagaagacc ataaaaccta ccctttttaa aggtgaaatc
     6481 cgcatcttgc aagatgactt gtcgcgtttt taaggtcaca tgagtaagct gcaacatatc
     6541 tagccctcct ttttctcact ctttaagaat taatagcctc cgctgtaata gtttatgacc
     6601 tcataacgag cgtagttcca gcctccgcca actttatact cagaatagct gtaataacga
     6661 gaccattccg gaatccaagc atactgatgg tcgtgatagt tgatactata ttccaaaacc
     6721 gtattccaat catacttgta acttttagtg gctgtcacag cagatacact ggactgaaga
     6781 agaccaatag attataaact aactaataaa acaacttttg ctgattttta atgattttat
     6841 atcctcaata ttgaatacgt tttcattata ccatttttat cattattatg caacaaattt
     6901 ttagtttatt tcactaatct acaacattta tccttcctat cgaacacttt cgattttcca
     6961 actatccaac cccttaaaac gtatagctcc ttgctggtca gttcgataaa ctttgctatt
     7021 gataccttcc agtcgtgtca atgtttcctg atgggggagt ttcattcgat tgctctttcc
     7081 aactgagata agagtaagct ctggtttgag tttttctaga aaggctggac ttgatgattt
     7141 tttatttcct tgttggctag ctttcaaaac atttactttc aagtctggat agtgcttcag
     7201 caagtccctc tctcctttct cctccaaatt tcctgtgaag agaaagtatt tatccaagag
     7261 tttcccgtat agaacaaggg aatcttcatg atctccatct ccaattttcc ttggagatag
     7321 gacttctaac tgacttccaa aaattggcaa gttctctcct gctgtcacat tgcgcacctt
     7381 ggtttgagtt acctgtagtt ctgccacaaa ttccttctgt ttcagactgt cttttgatac
     7441 tagaatctcc cctacatgga aagccttggt cacctctgac aaatctccaa catgctcctt
     7501 gtccgtgtta gtcaaaatta gctggtcaat cttagctact cctcgacttt tgagataggg
     7561 aatcaaggtt cgctgggcat tgctggtcgt catcttttct tgccattttt cgattttctt
     7621 ataagattct gccttgccac ctttgatacc aagcgaatca ttccctccaa ccattcaaag
     7681 ataaagttca gctgaatgac tggatataga aaggaaaggg caaataagat agacaagagc
     7741 ggtaagaaga ccaagtcaaa aagaaaggaa aagacaaagg tcaagaggat ggaccaaggt
     7801 tgaaattccg caaaatagaa ggacagaatg ggcaatatgc ccaaggagat gactagactt
     7861 tcactagcaa cagccttgag cccctcccct tctttgctgg tcatggtcag gataaaagca
     7921 taagcacagg acaagactcc tcctgctgtc aagaaaaagt ttggcatgac aataaagagg
     7981 acaagcaccg tcaaggcaaa attatccaag cccttaaccc catgttgagc cagtagcttt
     8041 tgcaagagac tgcgaataac cgatgctgaa aatccagtta gtcccgcata gataagggaa
     8101 aagggataag tcagccactt caacttttct tgtgtcaagc ccaatcgtaa aagtagtttc
     8161 ttaaatccgt ccatgaaaaa ccctacctgc atacctgaca aggcaaaaag gtgaataatt
     8221 cctaaactgg aataaagctc attcatctcc tcaaagtcgg tgtccagatg tcctagcaag
     8281 agtcctgtca tgtaattgcg catagggtct gggaagtgca tcttaatcca aaccacagcc
     8341 tttcgacgta aactggacag gttttcacct atatcccaac tgccaacctt ttggagtgac
     8401 tggattcttt taatattgag tgtctggtaa attccctgag tcttcagata gccttggtag
     8461 tcaaagccac caaaatttct ctgcccttct ggctccgaaa gcttcccttc tagtcctatc
     8521 tcatgaaggt cagttaaagc ttgaaaggct tctttctcct cctcggactg gagtttataa
     8581 tagacttgga aagcacgacc atcagactta ccacgaaagg acagactgtc accgttaacc
     8641 ttaatagtgt ctggtaaaat ccgtaccctt tcaacagaat ccaccaaatt ttgactcact
     8701 tgactctgtt gccaagtttg aaacagaaac cagaagccaa agactccaca aatcactaga
     8761 actttacctg ctgatttcca aggaaattgg ataaagaggc agactagcag aaaaacaaaa
     8821 cccaacaaag caaaatagga tactgagaaa atggcgtagt aaagccaaag taacagaaag
     8881 ctcatgtaaa tcaggggaat ggggaaattt ttaatccact gtaacataat ctttaagctt
     8941 ttctatggtc tttgcaccaa ttccagatac cttcttgagt tcatcaaccg acttaaattt
     9001 cccatttgtt tcacgatggt cgataatgtc ttgagctcgt tttcctccca gacccttgac
     9061 ctgcttgagt tcttccagac tggccttgtt gagattgacc ttcttgtcct tgcttgttga
     9121 agaaggcgct ccagaacctg cctgttgact agctgattct tctcccttag tgggaacgta
     9181 aaccagagcc tcgtcactaa ctttctgagc tagattgagc gacttactat ctgcttgttc
     9241 tgtcaagcca cctgctttct gcacggcatc attgactcta ctacctaccg gcaaatcgta
     9301 aatacctgga gatttgacag cacctttgac atctactgtg attagatctt gttcaagggg
     9361 ttcttccttg ttcacttcct tttcggtcga tgaatccttg gaaacagctg caacttctgc
     9421 ctgcaaattc gtttccttga caggtgtttg tggagctggc tttagcagga aaaatccgcc
     9481 tacaagcaag cccagaccag tacagatgac gatgatttta tactctttga ttttctcgat
     9541 aattgcttcc atattttctc ctctcttaga ttattcgtaa gaggaagaaa aaacagtcga
     9601 aaaatttctt ttcaactgct tatttatttg caaaatagtc ttccttggtc aagacataat
     9661 gaactcttgt cacgattcgg cctttttcat gctggtccat acaagcatat ggttctgcat
     9721 gggaaaaacg catgcctgat ttctccatga cctttcctga cgcaggattg tccttatcgt
     9781 gaagggcagt caacttattc atccctatct tctcaaaagc tagctcaatc acagcacgat
     9841 tggcttctgt cgttaatcct tgattccaat actttttatt gataatgtag ccaatagctg
     9901 ccttcttaag aacagaatca atcttgtgca agtcaatggt tccaataaac tgaccattgc
     9961 tttttagttc tattccccaa cgtcccaagg gattagccaa atagaactga gcaatgttat
    10021 tcttggtttc ttccaagctt tgattggttg gaaaaatgta acgtgtatta cccttgtccg
    10081 aggcatagtc aaacattgct tccgcatcat ccaaagttac aggtctaagc aataaacgct
    10141 ccgtttctat agacggatac tgggcaagtt ttacaaaaat tgattccata cttttccaac
    10201 caatcaaagc ttgattttca ctagtctagc ataaaaaatt tggatgacaa gtttttcttg
    10261 atttttgaag cggtttcata tacaatgaaa ccgtcacatt tttgatttat gaactggaat
    10321 gtgacaacca aaacaagacc gtcaattgaa aggtaggtta tctcaatgtt aatcggaatc
    10381 ccaaaagaaa ttaaaaataa cgaaaaccgt gtcgccctca cacctgcagg tgttcatagc
    10441 ttagttagtc gtggtcatcg tgtccttatc gaaacaaatg ctggtctcgg ttctggcttt
    10501 actgatgctg actatcaaaa gcaaggagct gagattgtcg ctactgctgg tgaagcttgg
    10561 gcagcagagt tggttgtgaa agtaaaagaa cctttaagtt ctgaatacgg ttacttgcgc
    10621 gacgatcttc ttctcttcac ctacttgcac atggccgctg ctccagaatt agcagatgct
    10681 atgttagcag caaaaacaac aggaattgcc tatgaaactg ttcgtgacaa tcaaggacaa
    10741 ctaccgctcc tcgttcctat gagtgaggtt gcaggtcgta tggctgttta aatcggagct
    10801 cacttcctta ctaagcaagc tggtggctct ggtgttctac ctggtggtgt accaggtgtt
    10861 ccaaaaggaa aagtaactat catcggtgat ggtgtcgtcg gtacacatgc tgcccgcatc
    10921 gcccttggtc ttggtgctca agtgactatt ttagatatta gtgccaagcg tctctcagtt
    10981 ctagaagaag tctttggaag tcaaattcaa actcttatat ctaattcatt caacattgaa
    11041 gcaagtgtga gagatgctga tgtggtgatt ggagccattc tcatccctgg tgcaaaagca
    11101 ccggaattgg tgacagatga gatggtcaaa caaatgcgtc caggctctgt atcgttgacg
    11161 ttgctgttga ccaaggtggc cttatcgaaa cagctgaccg tgtgacaacg cacgatgaac
    11221 ccgtctatga aaaacacggt gttctccact atgccgttgc caatatccct ggtgcggttg
    11281 cccgcacttc aaccatcgcc ctaaccaatg tcactcttcc ttatatcgaa gctttggctg
    11341 gcaaaggatt cgcacaagca atctctgaag atgaaggctt gcgtcaaggt gtgaccactt
    11401 atcaaggtta cttgactagc ctaccagttg ctcaaggact taatcgtgac tacactgata
    11461 tcaatgattt agtataaatt ttgaaaagaa aagggtgact tcgcaagtcc tacccactgt
    11521 gctttgtgtc atcagctatt acatcgttgg tttattgtac tgtagacttt gcaactggag
    11581 actttcgttc cagactcaat tgtgcataag aaactctaaa ttgaataaag tcaacagagt
    11641 ttcgcccaac cacttcgtct tgtaatgaca attatataaa ccaaaggaag ctgagctctt
    11701 tagtcccagc ttctttttat atataaaatt ttacccgtgc aaagacaggg ccttagcaga
    11761 cttctttttt acttcgttca cccttgcttt ttctttttag gtttgggcgt tggcagttgg
    11821 ttatacatag ctaaaatcag gtcttataga aacatcttat tatcaagttc ttccactcaa
    11881 atcatttctt tggcaccttt gtatggaaac tcaaaagaag attggtcaat cttatctaag
    11941 actgcttgca cgggtttaac taaaagcgat cgtcataaat accgccaata atcttgccgc
    12001 ggaagtaaag aatatactcc cccatcatgg aacggtaagt cacatcatct aatcctgata
    12061 attgttccaa aacaaattcc aaatagttct tacttgatgc catttctaat cttctaggct
    12121 ctgttcaacg ataacaaccg tatagagttc ttgcttaacc tcgcatccaa ttgatttaaa
    12181 gccctgcttt tcccaaaaat gctgagattg cggatttccc ttaacataag ccaaacgtgc
    12241 ctttcgaaag ttcttagcaa aataagctag tgcttctgtc acaatatgac taccaatccc
    12301 tttcctctga taggcttgat caaccataaa caaaccaata aaaacagtct cctcatcagg
    12361 atatgcatag acaaaatcca taacagccac aaggtcagat ccattccaaa atccaacaaa
    12421 aaacttatca gccttagctt taccttcagg tagacaaagc atgtcctctt ttacagttgc
    12481 aaaatttggt tctggtggac aatgctgaaa atacagagga ttactttcat ataaagataa
    12541 aatacttgga atatcctttt cagttagtat cctacaactg taatacttag atagttggtc
    12601 aatcatcttt tcaaattcct actttcttgt gccctgtgat tatgacacag gaagatgcac
    12661 tgatcgtcat cagccacata aaagttcttt ccatcgtgcc taatcgttgt ctcagacctt
    12721 tggataaaac ctttagccta tacaactgga ttttcctctc tcaaaagtat attcttttgc
    12781 aggcgaactt cctcaaaatc agtcgtgtgc aacttcagta gaatattcat aggctcggat
    12841 aatctgagcg acaactggat ggcgaaccac atccttggct gaaaaatgaa caaagtcaat
    12901 ctgatggatg ttcttgagtt tctcttgagc atcaatcaaa ccggacttga cattacgtgg
    12961 caggtcaatc tgactgatat ctccattgac aatcatctta gaatgaaagc ctaaacgcgt
    13021 caagaacatc ttcatctgca tgatggtcgt gttttgcgcc tcatcgagaa tgacaaaggc
    13081 atcatccaag gtccggccac gcatataggc aaggggcgca atttcgataa tttcacgctc
    13141 catgagacga gtcgtttggt ctttcccaag aatttgatac aaggcatcgt aaacaggacg
    13201 aaggtaagga tccaccttct ccttaagatc acccggaaga aatccaagac tctctcccgc
    13261 ttccaccgct ggacgagtta ggataattcg cttgacttgc ccacgtttaa gggcagtcac
    13321 tgccaaggtc actgcaagga aggtcttccc tgtacctgct ggcccaattc caaaggtcac
    13381 atcatgctgt ttgacactgt ccacataaag cttttgccct agggttttga cacggatagg
    13441 tttcccagta ttatctttga taatttcttc ttcgtaaagg gcgacaaact tgtcaatttc
    13501 atcatttttg accatgctaa tcgcagtgac tacatctggc gtaccaacgg tcatcccacg
    13561 atttaccaag accatcaaag cctgaataac ttgacgggct tcctcacagg cagactcttc
    13621 tcccaaaacc tggacaatct ccgtacgagc atgaatcaca acatcaagct cttcttccat
    13681 caaacgaaga tggcgttcat tggaaccaaa aagatgaaac aggtcatctg gatgactcag
    13741 ttgaatgtct attgaatgtt ccttcaaata aagaacctct ctaatccttt tatttctttt
    13801 tattatagca gagggatgga gaaattacta gcccaaaccc aatgaataaa tgctagtgtt
    13861 ctagatattc ctgccaaatg ctatcaaaat ctcctaggtt aaaagagaaa ctggcatgct
    13921 cctccaaaaa gcgactgacc tcatcaaaat catctgtatg ttttggaaaa gctgcctctt
    13981 caaaagcgag gtctgccaaa attgctttgg gactgttact tttaggattg cgctcggtca
    14041 tgagccaagt gtaaaatgat tttctcataa gcctccctaa atcaactctg tcccaaactg
    14101 gtcctgcttg attttaccag ccttcataag accacctaaa gctttcttga actgaccttt
    14161 agaaatgcca aaggttgcct tgatgtcgtc tggagatgac ttatcattta aggtcatgaa
    14221 accgccatta ctttccaaat aagtcaaaat catctgagca tcgttttcca acatttcaaa
    14281 ggagcgtggt ttgagggaga ggttcagagt gcggtccact tcacggaaac caataacgcg
    14341 cgcatctaat acttgcccca aacgtggctc tgcgtaacgc tcgctaggat gaataaaacc
    14401 aagcatatta ttttctggta ggtaaacaaa agttcctgac agcttgagac ggtaaacaat
    14461 ggctggccag ttttggttct gcatgttgtt gtaagcagga cgagcaagac gttggaagtc
    14521 ttcttgataa gccaagaggc cccagatacg gtctttctta tccacttcaa gacggatgta
    14581 gagttggtcg cccttcttag gccagagttc cttgagctca gggagaatat cgagtgacac
    14641 aacgatttcc ttgtcaggaa ggcctgtatc cacaaagaca cccaaatcct tacgaacctc
    14701 tgtgacacgt ccccaaccaa attggtcctg agtggcagtc acttctaagg ttgtcaggcg
    14761 gagtttttgc ttcatatccg tgtatgcaaa acctttgacc gtatccccta ctgtatgttg
    14821 accttcttcc ttagcaagag cataggtttg accatccttt tgcacaaagt aaaaacggtc
    14881 attttcatcg atgatcagtc caacgataaa acttgcaaga tttgtattca tatttccttc
    14941 tttcgaataa aactcagcca gcaatgccaa ctgagttttt ctgtttattt ttagacttcc
    15001 aaaagttctt tctctttgtt agcagtcatg tcgtcgatgt gtttaacagc atcgtctgtt
    15061 actttttgaa tgtctttttc aagagtcttc aattcgtctt cagtgatttc ttttgctttt
    15121 tcttgtttct tagcttcgtc catagcatcg cgacggatat tgcgaacagc cactttagca
    15181 ttttcgccga ccttcttcac ttctttagca aggtcacgac gagtttcttc tgtaagagct
    15241 gggataacca agcgaatcac agaaccgtca ttagccggtg tgataccaag atcagaagcg
    15301 ttcaaggcac gttcgatgtc tttcaatgaa gacttgtcaa atggtgttac caacaaaaca
    15361 cgcgcttctg gaatcgtaat tgaagcgatt tggttaagag gagtttcgac tccatagtat
    15421 tctacatgta tacggtcaag caagcttgca ttggcacgac cagcacggat accaccaaat
    15481 tcacgagcaa gtgattggtg agactgggtc attctctctt tagctttttc aataattacg
    15541 ttagccatat tctttcttat tccttttctt cgatattatt tgaaactgtt gttccgatat
    15601 tttcaccaaa tacgacacgt ttgatgtttc ctggttggtt catgttgaag acaaccaagt
    15661 caatgtcgtt atccattgag agggttgaag ctgttgagtc catgatacga agacctttgt
    15721 tgataacatc acggtgggtc aattcttcaa acttaacggc tgtcttgtcc ttcttaggat
    15781 cagcattgta cacaccatcg acgccatttt tagccatgag gatggcatct gcttcgattt
    15841 cagctgcgcg aagagccgct gttgtatctg tcgagaagta tggtgaacca attccagcac
    15901 caaagataac gatacggcct ttttcaaggt gacgaagggc acgtccacgg acataaggct
    15961 ctgccacttg ttgcatggca atagctgttt gtacacgcgt atcaacccca acttgttgca
    16021 atgaatctgc catcacaaga gcattcataa cagtcccaag cattccagtg taatctgcct
    16081 gaacacggtc catacctgct tctgctgcag gttctccacg ccagagattt cctccaccaa
    16141 taacaagggc aatttcgata cctaagctat gaacttcttg aatctctttt gcgattgttt
    16201 gaactgtttg gatatcaatc cctacgccac gttcaccggc aagggcttca cctgataact
    16261 tgattaaaat acgtttatac ttgggattcg ccattttcac tctccttctt tcatcctacc
    16321 tattttatca caatttctaa gatttttata gtatcatgaa caattctttc aaaaaaatta
    16381 gacagtcaaa aattcctcta agtcggcaag ggcacgctct gcaatttttt cataacgagc
    16441 cttcttatca cggatacgct cgccttccaa ctccttgatg atcccaaaat tgacattcat
    16501 tggttggaaa tgtttgctgt cggcatgggt aatgtaatga gctaagcttc caatcgctgt
    16561 cgtctcgggg aaaataacct cgctttcttc cttgaagaga cgagctgcgt taattcccgc
    16621 aactaagccc gaagccgccg actcaacata gccttccacc cccgtcatct gaccagcaaa
    16681 gaagagattt ggttgtttct tggaacggta agtctgctca agaagatttg gtgaatccat
    16741 gtaagaattg cgatgcatga caccataacg gacaaactcc gcattttcaa gacccggaat
    16801 catttggaag acacgctttt gttctcccca tttgaggtgg gtctggaaac caacaatatt
    16861 gtataggcta ccagctgcat tatcctgacg aagttgcaca accgcataag gtgttttaaa
    16921 ttctccatca cgaggtcctg tgtagtcgtc cggatactca agaccgactg gcttcatagg
    16981 gccataaagc atagttttaa tgccacgttt ggccataact tcgattggca tacatccttc
    17041 aaagtacttt tctttttcaa aagaattgag cggtgcttct tccgcattga ccaaggcttc
    17101 atggaaatcc ataaactctt gcttggtcat tggagcattg agataggctg cttctccctt
    17161 atcataacga gatttgagat agaccttact catatcgata gtgttgacat cgataatagg
    17221 cgctgctgca tcgtagaaat agaagccatc gccattatta agggcatgaa tcttttcagc
    17281 cagggcatcg ctagtcaagg gaccagtcgc cacaactgta ataacatctg ttggcaattc
    17341 tgtaatttca tcacgcacca cttcaatcaa ggggtggttg accacttttt cggtcaccat
    17401 ttgagagaaa ccatcacgat ccaccgcaag ggctccaccg gcaggaacac gtgtagcctc
    17461 agcagattcc aagataacag aacccaagcg acgcatttct tccttgagaa gaccaactgc
    17521 atttgtcaaa gcatccccac gcaaagaatt ggaacaaacc aactcagcaa aattgtctgt
    17581 tttgtgctga ggagtcgact tgacaccacg catttcataa agtttaactg gaataccacg
    17641 ctctgcgatt tggtaagctg cttcagaacc tgccaaacca gcaccgataa cattgatata
    17701 agattgagac acgacactaa tacctctttg ggagtgtgaa gttaagattc acattgaaaa
    17761 agccaatcag acttacgagc tttcgagttt cttggctcag gctgaaaaag tccacaggac
    17821 tttttcactc ccacaaatct ttctattttt tcttctacta gtataacaaa aaaagggaag
    17881 aaggcaaact tccctgttta gtcattttct tgatgtaaag agatagtgag tattccagtt
    17941 aagaatcaat tatatgctac tctataaaat cttttccaca taacggatcg atagggactg
    18001 ttattctatc tcttgctata accgtattat ctaaaacagc ataacattca acatagtgat
    18061 ctcctttaaa ctgtgaatct tcgtgatatt ttattttacc tgaaaaaata gcaccacgct
    18121 cacaatttct tctaatagcc tcagcttcaa tatttcttac tttccaatag acttcctgcg
    18181 aaacaaaata tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc
    18241 acgatttaag cgttttgttg gtgttcagcg cacgactttt gaagagatgt tagctgtatt
    18301 aaaaacagct tatcaactta aacatgcaaa aggtggacga aaacctaaat taagcctaga
    18361 agaccttctt atggctactc ttcaatatgt gcgagaatat cgaacttatg aagaaattgc
    18421 ggctgatttt ggtattcacg aaagcaactt actccgtcgg agccaatggg ttgaagtaac
    18481 tcttgttcaa agtggtgtta cgatttcaag aactcctctc agctctgagg acacggtaat
    18541 gattgatgcg acggaagtaa aaatcaatcg ccctaaaaaa agaattagcg aatgattctg
    18601 gtaaaaagaa atttcacgct atgaagactc aagcgattgt cacaagtcaa gg
//
LOCUS       DAVKHZ010000041        18299 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000041 DAVKHZ010000000
VERSION     DAVKHZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.041"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            91..5394
                     /locus_tag="VWW99_001286"
     CDS             91..5394
                     /locus_tag="VWW99_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9539124.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPLYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALILASVSLALSALFVVKTKKD"
     gene            complement(5561..7720)
                     /locus_tag="VWW99_001287"
     CDS             complement(5561..7720)
                     /locus_tag="VWW99_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9539125.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7717..8313)
                     /gene="recU"
                     /locus_tag="VWW99_001288"
     CDS             complement(7717..8313)
                     /gene="recU"
                     /locus_tag="VWW99_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9539126.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8379..8906
                     /locus_tag="VWW99_001289"
     CDS             8379..8906
                     /locus_tag="VWW99_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9539127.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8976..9305
                     /gene="gpsB"
                     /locus_tag="VWW99_001290"
     CDS             8976..9305
                     /gene="gpsB"
                     /locus_tag="VWW99_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9539128.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9326..9706
                     /gene="rnpB"
                     /locus_tag="VWW99_001291"
     ncRNA           9326..9706
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VWW99_001291"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9791..10948
                     /locus_tag="VWW99_001292"
     CDS             9791..10948
                     /locus_tag="VWW99_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9539129.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10960..12354
                     /gene="mapZ"
                     /locus_tag="VWW99_001293"
     CDS             10960..12354
                     /gene="mapZ"
                     /locus_tag="VWW99_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9539130.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12430..13854
                     /gene="gndA"
                     /locus_tag="VWW99_001294"
     CDS             12430..13854
                     /gene="gndA"
                     /locus_tag="VWW99_001294"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9539131.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13866..14555
                     /locus_tag="VWW99_001295"
     CDS             13866..14555
                     /locus_tag="VWW99_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9539132.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14653..15672
                     /gene="cbpC"
                     /locus_tag="VWW99_001296"
     CDS             14653..15672
                     /gene="cbpC"
                     /locus_tag="VWW99_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9539133.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIHGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNLLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVR"
     gene            complement(15790..17022)
                     /locus_tag="VWW99_001297"
     CDS             complement(15790..17022)
                     /locus_tag="VWW99_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9539134.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLKLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAYIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(17552..18027)
                     /locus_tag="VWW99_001298"
                     /pseudo
     CDS             complement(17552..18027)
                     /locus_tag="VWW99_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT         5650 a         3317 c         4143 g         5189 t
ORIGIN      
        1 cagggttaag cattgcactt tcagaggaag gagtattttt taaaaagaaa atgtaaacgc
       61 ttgctaaaaa atgaaaggat ttagaagttt atgaataaag gattatttga aaaacgttgt
      121 aaatatagta ttcggaaatt ttcattaggt gttgcttctg ttatgattgg agctgcattc
      181 tttgggacaa gtccggttct tgcagatagc gtgcagtctg gttccacggc gaacttacca
      241 gctgatttag ctactgctct tgcaacagca aaagagaatg atggacgtga ttttgaagcg
      301 cctaaggtgg gagaagacca aggttctcca gaagttacag atggacctaa gacagaagaa
      361 gaactattag cacttgaaaa agaaaaacca gctgaagaaa aaccaaaaga ggataaacct
      421 gcagctgcta aacctgaaac acctaagacg gtaacccctg aatggcaaac ggtagagaaa
      481 aaagaacaaa agggaacagt cactatccga gaagaaaaag gtgtccgcta caaccaacta
      541 tcctcaactg ctcaaaatga taacgcaggt aaaccagccc tgtttgaaaa gaagggcttg
      601 accgttgatg ccaatggaaa tgcaactgtt gatttaacct tcaaagaaga ttctgaaaag
      661 ggcaaatcac gctttggtgt cttcttgaaa tttaaagata ccaataataa tgtttttgtc
      721 ggttatgaca aggatggctg gttctgggaa tacaaaactc caggtaatag cacatggtat
      781 aaaggcaacc gtgtagttgc accagaaccg ggttctgtaa accgtctttc tatcactctc
      841 aagtcagatg gacaactcaa tgccagcaat aacgatgtca atctctttga cacagtgaca
      901 ctgcctgggg cggtcaatga taatcttaaa aatgagaaga agatccttct caaggcagga
      961 acttatggca atgaccgtac ggttgtcagc gttaaaacgg ataatcaaga aggcgtaaaa
     1021 gcggatgata ctcctgccca gaaagaaaca ggtccggttg ttgacgatag caaggtgact
     1081 tatgatacga tccagtctaa ggtacttaag gcagtaattg accaagcctt ccctcgtgtc
     1141 aaggaataca ccttgaatgg acatactttg ccaggacagg tgcaacagtt caaccaagtc
     1201 tttatcaata accaccgaat cacccctgaa gtcacttata agaaaatcaa tgagacaaca
     1261 gcagagtact tgatgaagct tcgcgatgat gctcacttaa tcaatgcgga aatgacagta
     1321 cgcctgcaag ttgtggacaa tcaattgcac tttgatgtga ccaagattgt caaccacaat
     1381 caagtcactc caggtcaaaa gattgatgac gaaagaaaac tactttcttc tattagtttc
     1441 ctcggcaatg ctttagtctc tgtttctagt gatcaaactg gtgctaagtt tgatggggca
     1501 accatgtcaa acaatacgca tgtcagcgga gatgatcata tcgatgtaac caatccaatg
     1561 aaagacctag ccaagggtta catgtatgga tttgtttcta cagataagct tgctgctggt
     1621 gtttggagta actctcaaaa tagctatggt ggtggttcga atgactggac tcgtttgaca
     1681 gcttataaag aaacagtcgg aaatgccaac tatgtaggaa tccatagctc tgaatggcaa
     1741 tgggaaaaag cttataaggg cattgttttc ccagaataca cgaaggaact tccaagtgct
     1801 aaggttgtta tcactgaaga tgccaatgca gacaagaaag ttgattggca agatggtgcc
     1861 attgcttatc gtagcattat gaacaatcct caaggttggg aaaaagttaa ggatatcaca
     1921 gcttaccgta tcgcgatgaa ctttggttct caagcacaaa acccattcct tatgaccttg
     1981 gatggtatca agaaaatcaa tctccataca gatggtcttg ggcaaggtgt tctccttaaa
     2041 ggatatggta gcgaaggcca tgactctggt cacttgaact atgctgatat tggtaagcgt
     2101 atcggtggtg tcgaagactt caagacccta attgagaagg ctaagaaata tggagctcat
     2161 ctaggtatcc acgttaacgc ttcagaaact tatcctgagt ctaaatactt caatgaaaaa
     2221 attctccgta agaatccaga tggaagctat agctatggtt ggaactggct agatcaaggt
     2281 atcaacattg atgctgccta tgacctagct catggtcgtt tggcacgttg ggaagatttg
     2341 aagaaaaaac ttggtgacgg tctcgacttt atctatgtgg acgtttgggg taatggtcaa
     2401 tcaggtgata acggtgcctg ggctacccac gttcttgcta aagaaattaa caaacaaggc
     2461 tggcgctttg cgatcgagtg gggccatggt ggtgagtacg actctacctt ccatcactgg
     2521 gcagctgact tgacctacgg tggctacacc aataaaggta tcaacagtgc catcacccgc
     2581 tttatccgta accaccaaaa agatgcttgg gttggggact acagaagtta tggtggtgca
     2641 gccaactatc cactgctagg tggctacagc atgaaagact ttgaaggctg gcaaggaaga
     2701 agtgactaca atggctatgt aactaacctc tttgcccatg acgtcatgac taagtacttc
     2761 caacacttca ctgtaagtaa atgggaaaat ggtacaccgg tgactatgac cgataacggt
     2821 agcacctata aatggactcc agaaatgcga gtggaattgg tagatgctga caataataaa
     2881 gtagttgtaa ctcgtaagtc aaatgatgtc aatagcccac tataccgtga acgtacagta
     2941 acgctcaacg gacgtgtcat ccaagatggt tcagcttact tgactccttg gaactgggat
     3001 gcaaatggta agaaactttc tactgataag gaaaagatgt actacttcaa tacgcaggcc
     3061 ggtgcaacaa cttggaccct tccaagcgat tgggcaaaga gcaaggttta cctttacaag
     3121 ctaactgacc aaggtaagac agaagagcaa gaactaactg taaaagatgg taaaattacc
     3181 ctagatcttc tagcaaatca accatacgtt ctctatcgtt cgaaacaaac caatcctgaa
     3241 atgtcatgga gtgaaggcat gcacatctat gaccaaggat ttaatagcgg taccttgaaa
     3301 cattggacca tttcaggcga tgcttctaag gcagaaattg tcaagtctca aggggcaaac
     3361 gatatgcttc gtattcaagg aaacaaagaa aaagttagtc tcacccagaa attaactggc
     3421 ttgaaaccaa ataccaagta tgccgtttat gttggtgtag ataaccgtag taatgccaag
     3481 gcaagtatca ctgtgaatac tggtgaaaaa gaagtgacta cttataccaa taagtctctc
     3541 gccctcaact atgtaaaagc ctacgcccac aatacacgtc gtaacaatgc tacagttgac
     3601 gatacaagtt acttccaaaa catgtacgcc ttctttacaa ctggatcgga cgtatcaaat
     3661 gttactctga cattgagtcg tgaagctggt gatcaagcaa cttactttga tgaaattcgt
     3721 acctttgaaa acaattcaag catgtacgga gacaagcatg atacaggtaa aggcaccttc
     3781 aagcaagact ttgaaaatgt tgctcagggt atcttcccat ttgtagtggg tggtgtcgaa
     3841 ggtgttgaag ataaccgcac tcacttgtct gaaaaacaca atccatatac acaacgtggt
     3901 tggaatggta agaaagtcga tgatgttatc gaaggaaatt ggtcactcaa gacaaatgga
     3961 ctagtgagcc gtcgtaactt ggtttaccaa accatcccac aaaacttccg ttttgaagca
     4021 ggtaagacct accgtgtaac ctttgaatac gaagcaggat cagacaatac ctatgctttt
     4081 gtagtcggta agggagaatt ccagtcaggt cgtcgtggta ctcaagcaag caacttggaa
     4141 atgcatgaat tgccaaatac ttggacagat tctaagaaag ccaagaaggc aaccttcctt
     4201 gtgacaggtg cagaaacagg cgatacttgg gtaggtatct actcaactgg aaatgcaagt
     4261 aatactcgtg gtgattctgg tggaaatgcc aacttccgtg gttataacga cttcatgatg
     4321 gataatcttc aaatcgaaga aattacccta acaggtaaga tgttgacaga aaatgctctg
     4381 aagaactact tgccaacggt tgccatgact aactacacca aagagtctat ggatgctttg
     4441 aaagaggcgg tctttaacct cagtcaggcc gatgatgata tcagtgtgga agaagcgcgt
     4501 gcagagattg ccaagattga agctttgaag aatgctttgg ttcagaagaa aacggctttg
     4561 gtagcagatg actttgcaag tcttacagct cctgctcagg ctcaagaagg tcttgcaaat
     4621 gcctttgatg gcaatgtgtc tagtctatgg catacatctt ggaatggtgg agatgtaggc
     4681 aagcctgcaa ctatggtctt gaaagaacca actgaaatca caggacttcg ctatgttccg
     4741 cgtggatcag gttcaaatgg taacttgcga gatgtgaaac ttgttgtgac agatgagtct
     4801 ggcaaggagc atacctttac tgcaactgat tggccagata acaataagcc aaaagacatt
     4861 gattttggta agacaattaa ggctaagaaa attgtcctta ctggtaccaa gacatacgga
     4921 gatggtggag ataaatacca atctgcagcg gaacttatct ttactcgtcc acaggtagca
     4981 gaaacacctc ttgacttgtc aggctatgaa gcagctttgg ttaaggctca gaaattaaca
     5041 gacaaagaca atcaagagga agtagctagc gttcaggcaa gcatgaaata tgcgacggat
     5101 aaccatctct tgacggaaag aatggtggaa tactttgcag attatctcaa ccaattaaaa
     5161 gattctgcta cgaaaccaga tgctccaact gtagagaaac ctgagtttaa acttagatct
     5221 ttagcttccg agcaaggtaa gacgccagat tataagcaag aaatagctag accagaaaca
     5281 cctgaacaaa tcttgccagc aacaggtgag agtcaatctg acacagccct catcctagca
     5341 agtgttagtc tagccctatc tgctctcttt gtagtaaaaa cgaagaaaga ctagtattta
     5401 gtaaaacctc ttaacaagat tacggaagca gtctctatct tttccaatga ggtttatagt
     5461 acagaaaaag cctgagaaga tgtcttctca ggcttttgtt aagcacataa atacaatagt
     5521 gctatgacaa aatcacccag aaaaatctgg gtgataaatg ttatggttgt gctggttgag
     5581 gattctgatt ttgttgatca ggggttgtat ttgattgttg cgtattattg ttaggattgg
     5641 tagtcgtact attatttgtg cttggagtgg ttgagctaga ctgtgaagtt gaactatctg
     5701 atgatgagct tgaactttca gttgatgggg gttgttgtgg agcaggtgag ctccacgtag
     5761 aacgagcacc atttttaaat acgaattctc catttctgta gagcccctct ggtatattcc
     5821 aatcctctgg attgcttcct tcagacaggt aggtcatcat agagcggtaa actttggcag
     5881 cgaccgtaag gccattgcct acaagtggtg tcagacggtt agaatagcct gtccatacag
     5941 ccattgaata tttacgcgta tagccagcaa atagttcatc aggtgctaca aattgagagg
     6001 tcttgatgtg gttttcaatt tcctcgtctg tatagttaga ggttcctgtt ttaccagcct
     6061 gagggagcca agcaagatag gcatttcgtc cagttccata actcaagact gttttcatca
     6121 tgtcggtcat catataggct gtcgtttcct tcatggcacg agttccgaca ttagagaact
     6181 ctttttcact cccatcacta aagacgactt tatggatata cattggttta tagtaagttc
     6241 caccatttgc aaaggcagcg taagcagcag ccatcttttc actacttgct ccatattttt
     6301 tgtctgattc ggttgtgtta cttgaaatgg catttgagta gtgaatactt gggtagtcga
     6361 ttccgagacc atttaggaaa gtcttggcgc ggttgagtcc gaccttgttt agagtttcca
     6421 cggctgggac gtttcgcgat tgttgcaggg cgtattgcaa ggtgatgttg ccaaagtagc
     6481 ccctatccca gttataaaca ggggtatttg tcccagggta gttatagggt tcatcgtgaa
     6541 cgatagtagc agttgaatcg tagacaccgt actccaaggc aggagcatag tctgtgatcg
     6601 gtttcatagt tgatccccag tcgcggtttg tttctactgc ttggttaatt ccgaaggaaa
     6661 cattgcttga ctgatggcgt gctcctagct gggcaatgac tttaccgtta gaaacatcaa
     6721 caatggtaga agcgacttgc aattcatcgt ctggataggc aacgtattcg tctgtattgt
     6781 aaatatccca cagatgtttt tgagcttctt ggtctacatt tgtgtagaca tccatcccag
     6841 ttgtgagcag gttatagcct gtttcttctt caacttgatt gatgacttcc ttgaggtaat
     6901 tatccatgta agcagggtaa ttacttgctg atttgagact ttgtagtcca tcagtaattg
     6961 gtgtattgac tgctttctca tactgttcag cagagatgta gccttgattt ttcatttcag
     7021 ataagaccaa gtttcggcgg tcttgggctg cttctggatg tgaatagggg tcatattggt
     7081 ttggtgcctg aggcattcca gccagcaagg ctaactgagg taaacttaaa ttattgaggt
     7141 ctttaccata gtagttttga gctgctgtct gcattccata gttcccatta gacatgtaga
     7201 ccttatttat atagtaggtc aagatttctt gcttggttgc tttttgttct aactgaatcg
     7261 ctaaccaagc ttcctgagcc ttacgagaaa tagtctggtc ggaagtcgaa gttgaaaagt
     7321 aagtcaactt aatcaactgt tgagtgagag ttgatccacc ttggagggaa ttgctttgca
     7381 gattgcgcaa gaaagctccc aggatacgga tggtatcaat ccccctgtgg tcgaagaagc
     7441 gatggtcttc gatagaaacg attgctttaa ccaaatctgt gggaatatca ttagcttggg
     7501 cattgacgcg gcgttcagaa cccaagtcag caatgagttg atttttattg tcgtagattt
     7561 tactagaagt tgttgcaact agtttactct cggataggct aggagccttg ctaacgtagt
     7621 agaaaaaaac tcctccgcct aagacaatgg ctgcgataac caagcttaag aagctaatgc
     7681 tcagatactt gattaggcgc agaatcgttg gtttgttcat cttgttttac cacctaataa
     7741 atgttctttg ataacattga gataaggaat ttgagggaag gcaccagcct tgatttcata
     7801 tccatattct cgaatatatt caagtggcat tgatttttgt cccttatctt gatgatagaa
     7861 gcgaatcaaa tcgaatgccg gcaataagta ggtttcttgc tgagaagaaa agtgaagaag
     7921 gacaaagcag attccttgtt gggcaaggac ttgttccata tgctgaatct gatgtggatg
     7981 aaaatttttc atcggaatcg cacgtttttg ttttgtttcc ttgacttcaa agtcgatgta
     8041 atatccatta taaacgccag aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac
     8101 aatcttggca cgacttcgtt gtggatagtc cacttgtacg atttgaatag gagttggttt
     8161 cttatgtata acagccaagc cctgagacaa atagtagtcg ttggtagcat tgatcatctt
     8221 ttcaaaagac attcctcgat ttgcgaaatt tttgggttga gaaagagatg tttgtctttt
     8281 ttgtgatgaa actttatgtg gatagttgac cataattctc cttattggta caataacatc
     8341 actttattat accataattt gcacagaaag ggttaaaaat ggctacagct ttggttttag
     8401 gctattctgc ttttgatttg ggtttatttt ctgataagga tcctcgtctt aaattgatta
     8461 aaaaagcgat acgtaaagat ttagaggcta tggcagcaga tggggtgtct tggcttgtgt
     8521 ttacagggag tttgggtttt gaatattggg ttttagaggt tgctcaggag atgaaaaccg
     8581 aatacggttt ccagttggcc accatttttg cttttgaaac ccatggggaa aattggaatg
     8641 aaggcaatca gatgaaactg agtcgcttta agcaggtaga ctttgtcaaa tatgcttatc
     8701 ctcgctatga acacaagggg cagttaagag attaccagca gtttttgctg gaaaacacga
     8761 ctagttccta tcttttttat gatgaagaaa atgaaacaaa actagcttat ttttaccaaa
     8821 agatgaaaaa tcaagaggac tattttataa agagattaac atttgatcaa ttaaatgaac
     8881 ttgctgaaaa tttttccgaa aattgaagct ttgaccttga tttttgtttg ccttttttta
     8941 tataataata ctagcaagcg agaatggaga gagacatggc aagtattatt ttttcagcga
     9001 aagatatttt tgaacaagag tttggacgtg aagtccgtgg ctataataaa gtagaagttg
     9061 acgagttttt agacgatgtc atcaaggact atgaaaccta tgctgccttg gtcaagtcac
     9121 ttcgtcagga aattgcggat ttgaaggaag aattaactcg taaaccgaaa ccttcaccag
     9181 ttcaagcaga accccttgaa gcggcaatta caagttctat gacgaatttt gatattttga
     9241 aacgcctgaa tagattggaa aaagaagttt ttggtaaaca aattttagat aactcagatt
     9301 tttaagtagt tatttgagat gtgcaatttt tggataatcg cgtgaggaga attgcttctc
     9361 atgaggaaag tccatgctag cacaggctgt gatgcctgta gtgtttgtgc taggcgaaac
     9421 cataagccta gggacgagaa atcgttacgg cagttgaaat ggctaagtcc ttggataggc
     9481 cagagtaggc ttgaaagtgc cacagtgacg gagtctttct ggaaacagag agagtggaac
     9541 gcggtaaacc cctcaagcta gcaacccaaa ttttggtcgg ggcatggagt acgcggaaac
     9601 gaacgtagta ttctgactgc tatcagctag agctgttagt ggtagacaga tgattatcga
     9661 aggaagtggt cctagtcact tctggaacaa aacatggctt atagaaaatt gcatataggt
     9721 tggggctgag aaattttctc aacctcattt tttaaagtgg acatatagaa aggtcttgca
     9781 agactgtaac atgaaaaaag aatttaattt aattgcaact gtggcagcag ggcttgaggc
     9841 tgtcgttggt cgtgaagtgc gagagttggg ctacgattgt cagattgaaa atggacgtgt
     9901 tcgttttcaa ggagacgtga gagctattat cgaaaccaac ctttggctgc gggcagcaga
     9961 tcgtatcaaa attatcgtag gaacgttccc tgctaagact tttgaagagc tatttcaggg
    10021 agttttcgct ttagattggg aaaattattt accacttgga gctcggttcc cgatttcaaa
    10081 agctaaatgt gttaagtcca aacttcacaa tgagcccagt gttcaggcta tttctaagaa
    10141 agctgttgtc aagaaattgc agaaacacta tgctcgccca gaaggggttc ctctgatgga
    10201 gaatggccca gagtttaaga ttgaggtctc tattctcaaa gatgtggcaa ctgtcatgat
    10261 tgatacgacc gggtctagcc tctttaaacg tggttatcgt accgaaaaag gtggcgctcc
    10321 tatcaaggaa aatatggcag cagccatttt acaactttct aactggtatc cagacaagcc
    10381 tttgattgat ccgacctgtg gttcggggac tttctgtatt gaggcagtta tgattgctag
    10441 aaagatggcg ccaggtcttc gtcgctcttt tgcatttgag gaatggaact ggatcagcga
    10501 tcgcttgatt caagaagtgc gcacagaagc ggctaaaaaa gtagaccgtg agcttgagct
    10561 ggatatcatg ggctgtgata ttgatgctcg catggtggaa attgctaagg ccaatgctca
    10621 ggtagctggt gttgcaggag acattacttt taagcagatg cgcgtgcagg atttacgttc
    10681 cgataaaatc aatggagtaa tcatttccaa tccgccttat ggtgaacgtt tgtcagatga
    10741 tgcaggggtg accaagctct atgctgagat ggggcaagta tttgcaccgc tgaaaacttg
    10801 gagcaaattt atcctgacta gtgatgaagc ttttgaaagc aagtatggta gccaagcaga
    10861 taagaagcgt aagttataca acggaacctt gaaagtggat ctatatcaat attttggtca
    10921 gcgtgtcaaa cggcaagagg taaaatagaa aggatactca tgagtaaaaa aagacgaaat
    10981 cgtcaaaaaa aagaaggtca agaaccgcaa tttgattttg atgaagcaaa agagctaaca
    11041 gttggtcaag ctattcgtaa aaatgaagaa gtggaagcag gagtcttgcc tgaggattcc
    11101 attttggaca agtatgttaa gcaacacaga gatgaaattg aggcggataa gtttgcgact
    11161 cgtcaataca aaaaagagga gttcgttgaa actcagagtc tggatgattt aattcaagag
    11221 atgcgtgagg ctgtagagaa gtcagaagct tcttcggagg aagttccatc ttctgaagac
    11281 atcttactac ccttgcctct ggacgatgag gagcaaggct tggatcctct attgctagat
    11341 gatgaaaatc caacagaaat gactgaagaa gtggaagagg agcaaaacct ttctcgtctg
    11401 gatcaagagg actcagaaaa gaaaagtaaa aaaggcttta ttttgaccgt tttggcgctt
    11461 gtatcagtaa ttatttgtgt cagtgcttat tatgtctacc gtcaagtggc tcgttcgacc
    11521 aaggaaattg aaacttctca atcaactaca gccaatcaat cggatgtgga tgattttaat
    11581 acactttatg acgcctttta cacagatagc aataaaacgg ctttgaaaaa tagccagttt
    11641 gataaactga atcaactcaa gactttactt gataagctgg aaggtagtcg tgaacatacg
    11701 cttgccaaat ctaaatatga tagtctagca acgcaaatca aggctattca agatgtcaat
    11761 gcacaatttg aaaaatcagc tattgtggat ggtgtgttgg ataccaatgc caaagccaaa
    11821 tcggatgcta aatttacaga tattaaaact ggaaatacgg agcttgataa agtgctagat
    11881 aaggctatca gtcttggtaa gagccagcaa acaagtactt ctagctcaag tttaagtcaa
    11941 actagcagct caagttcaag tcaagcaagt tcaaatacga ctagtgagcc aaaaccaagt
    12001 agttcaaatg agactagaag tagtcgcagt gaagtcaata tgggtctctc gagtgcaggg
    12061 gttgctgttc aaagaagtgc cagtcgtgtt gcctataatc agtctgctat tgatgatagt
    12121 aataactctg cctgggattt tgcggatggt gtcttggaac aaattctagc gacttcacgt
    12181 tcacgtggct atatcactgg agaccaatat atccttgaac gtgtcaatat cgttaacggc
    12241 aatggttatt acaacctcta taagccagat ggaacctatc tctttaccct taactgtaag
    12301 acaggctact ttgtcggaaa tggcgctggt catgcggatg acttggacta ctaagcagtc
    12361 gttacaaaat tctttctttt caaaagtaaa aatgataaaa taaaacaaat gaaacaagag
    12421 gagtgtcaaa tgacaaaagc taactttggt gtcgtaggta tggccgtaat gggtcgtaac
    12481 cttgccctta atattgaatc acgtggttac acagttgcta tctacaaccg tagtaaagaa
    12541 aaaacggaag atgtgattgc ttgccatcct gaaaagaatt ttgtaccaag ctatgacgtt
    12601 gaaagttttg taaactcaat cgaaaaacct cgtcgtatca tgctgatggt tcaagctgga
    12661 cctggtacag atgctactat ccaagccctt cttccacacc ttgacaaagg tgatatcttg
    12721 attgacggtg gaaatacttt ctacaaagat accatccgtc gtaatgaaga attggcaaac
    12781 tcaggtatca actttatcgg tactggagtt tctggtggtg aaaaaggtgc tcttgaaggt
    12841 ccttctatca tgcctggtgg acaaaaagag gcctacgaat tggttgcgga tgttcttgaa
    12901 gaaatctcag ctaaagcacc agaagatggc aaaccatgtg tgacttatat cggtcctgat
    12961 ggagctggtc actatgtgaa aatggttcac aacggtatcg agtacggtga tatgcaattg
    13021 atcgcagaaa gttatgactt gatgcaacac ttgctaggtc tttctgcaga ggatatggct
    13081 gaaatcttta ctgagtggaa caagggtgaa ttggatagct acttgatcga aatcacagct
    13141 gatatcttga gccgtaaaga cgatgaaggc caagatggac caatcgtaga ctacatcctt
    13201 gatgctgcag gtaacaaggg aactggtaaa tggactagcc aatcatctct tgaccttggt
    13261 gtaccattgt cactgattac tgagtcagtg tttgcacgct acatttcaac ttacaaagaa
    13321 gaacgtgtat atgctagcaa ggtgcttcca aaaccagctg ccttcaactt tgaaggagac
    13381 aaggctgaat tgattgaaaa gatccgtcaa gccctttact tctcaaaaat catttcatac
    13441 gcacaaggat ttgctcaatt gcgtgtagcc tctaaagaaa acaactggaa cttgccattt
    13501 gcagatatcg catctatctg gcgtgatggc tgtatcatcc gttctcgttt tttgcaaaag
    13561 attacagatg cttacaaccg cgatgcagat cttgccaacc ttcttttgga cgagtacttc
    13621 ttggatgtta ctgctaagta tcaacaagca gtgcgtgata tcgtagctct tgcggttcaa
    13681 gctggtgtgc cagtgccaac tttctcagca gctattactt actttgatag ctaccgttca
    13741 gctgaccttc cggctaactt gatccaagca caacgtgact actttggtgc tcacacttac
    13801 caacgtaaag acaaagaagg aaccttccac tactcttggt atgacgaaaa ataagtaggt
    13861 cagccatggg gaaacggatt ttattacttg agaaagaacg aaatctagct cattttttaa
    13921 gtttggaact ccagaaagag cagtatcggg ttgatctggt agaggagggg caaaaagccc
    13981 tttccatggc tcttcagaca gactatgatt tgattttatt gaacgttaat ctgggagata
    14041 tgatggctca ggattttgca gaaaaattga gtcgaactaa acctgcctca gtcatcatga
    14101 ttttagatca ttgggaagac ttgcaagaag agctggaagt tgttcagcgt tttgcagttt
    14161 catacatcta taagccagtc tttatcgaaa atctggtagc gcgtatttcg gcgatcttcc
    14221 gaggtcggga cttcattgat caacactgca gtctgatgaa agttccaagg acctaccgca
    14281 atcttaggat agatgttgaa catcacacgg tttatcgtgg tgaagagatg attgctctga
    14341 cccgccgtga gtatgacctt ttggcgacac ttatgggaag caagaaagta ttgactcgtg
    14401 agcaattgtt ggaaagtgtt tggaagtatg aaagtgcgac cgagacaaat atcgtagatg
    14461 tctatatccg ctatctacgg agcaagcttg atgttaaagg tcaaaaaagc tacattaaaa
    14521 ccgtgcgtgg tgttggttac accatgcaag aatagaaaag cagttgcagt tgtgtaactg
    14581 cttttttgag gagtttttat atattgacat gcagtcgggt ctttgctaca atcagttatg
    14641 gaggatatat aaatgaaact tttgaaaaaa atgatgcaag ttctactagc agtctttttc
    14701 tttggtttgc tagctacaaa tacggtattt gcaaatacca caggtggccg atttgttgat
    14761 aaggataata gaaaatatta tgtaaaagat gatcataaag caatctattg gcataaaata
    14821 gacggtaaaa cttactattt tggtgatatt ggagagatgg ttgtcggttg gcaatactta
    14881 gaaattcatg gaacaggtta tcgtgataat ttattcgata accaaccagt taatgaaatt
    14941 ggccttcagg agaagtggta ctattttgga caagatggtg ctttgctaga acaaacagat
    15001 aaacaagtac tagaggcaaa aacgtctgaa aatacaggaa aagtatacgg tgaacaatat
    15061 cctctatctg ctgaaaagag aacttattat tttgataata attatgctgt aaagacaggc
    15121 tggatttatg aagacggcaa ttggtattat ttaaataagc taggaaattt tggcgatgat
    15181 tcttacaatc tactaccaat tggtgaagtt gctaagggtt ggactcaaga ttttcatgtt
    15241 actattgaca ttgatagaag caaacctgct ccatggtact acctagaccc agaaactggc
    15301 attatgcgaa caggatggca atatctaggt aataactggt actacctccg ttcatcagga
    15361 gcaatggcca ctggctggta tcaggaaggt accacttggt attatttaga ccagccaaat
    15421 ggcgatatga aaacaggttg gcaaaacctt gggaacaaat ggtactatct ccgttcatca
    15481 ggagctatgg caactggttg gtatcaagat ggttcaactt ggtactacct aaatgcaggt
    15541 aatggagaca tgaagacagg ttggttccag gtcaatggca actggtacta tgcttatagc
    15601 tcaggtgctt tggcagtgaa tacgaccgta gatggctatt ctgtcaacta taatggcgaa
    15661 tgggttcggt aatgacaggg cgcttataat tatattagaa cactcgctta atcaaagaag
    15721 tgactttgtt gtacttgacc tgacgattgg ggagattaaa agcatgtgaa atctacacat
    15781 gctttttcgt tagttatgag tgttgaactt gactaaccat tgacaaaaga taagagcaat
    15841 taaaataaga tacaaaaata tcaatgctaa ctgcaccgaa acgcccgatg ctatggatag
    15901 ggctaatagg gagagcaggg acggggcgag aatgtccact gtgctcagag cagagcccac
    15961 cgtccccatc ttgtcactgg ggatataggc aaagacagct gcttgtaggc gagggctgag
    16021 aatgccgata atcgtagaac tgatgaaact ggtcatgaaa attacgatat aggcttgaca
    16081 aaggatacta gttattagag ccaataggga aatctcacag aaaaccaaga ggtgcgtgaa
    16141 acggatattt ttaaatagat tactgctggt catattgcca aggatagctc cacaagacat
    16201 gatgatggag agcagggcga ggagttgccc aatgctcagt tgtgctagtg tctgttggat
    16261 gaagcggagg gcaatcagcc gaggggacac atccaagatg gcaatcatac tggtcgtcag
    16321 aaacagtagt atgacggtat cttttaactt cagtagcaca gccagcgact gtttaagatg
    16381 ttgaaaatat ttcttaaaac tcagtgctgt atgtgacatt tcaatccttt tttcaacctc
    16441 atacaaggta tgtcgaataa catacaggcc caaaaaggca atgacaaaag tcagagtgtt
    16501 gataagggaa atagtttgaa tacttataac attgataagg aatgcgccag ccagattggc
    16561 gacaatgcgg acaaccctca tcagagactg cctgaacccc atggcatcat gcaggtcgtc
    16621 cttaattaca ctgatgtaga gggcgttcat catgtaggca gacaggtagc tcaaggtgtc
    16681 agagatgagg ttgatgatga caatcataat gactgaaact aatgaaaagg gcgacaagac
    16741 gagaaatact gtcaaaatag cgtagagcag gattttgatt ttggcaatac agagtgcgtg
    16801 tttgactcta ttttcttgaa aatctcctag aatcccgaga aagagttgcg atagtagggg
    16861 aaccatctct gaaatggaaa tcagtaaaat cgctagcgga gcgaaattgt actgagccac
    16921 ataattgata atggcgaggt agaaaatact gtcgccgaat gatgaaaaac agctgtttag
    16981 cagcaactgt ctatatatgt tgtttgtcca aaataatttc atacatttct ccttatgtct
    17041 gcacatcgaa tgggaattta ttatttaata gacaccaata tacccccatg cttcctattc
    17101 cgaagtcaaa caaatctgct ttaccactat ctccttgaaa catcagtaaa ccgtgatcat
    17161 ctcgttcaga acatgctaaa attacctgtt ggattttttc atcagtaatt tgttttgatt
    17221 gtagacagtg gtttgtagta agctagttat tccgtggcaa tatcctgtca tcatttttag
    17281 atgatggtta aaaacaaatt cttgatattt actaataata tctttgtttc cgtcacaatc
    17341 gtacatacaa agatataaga ttattccgga aattcctttg caccaagaaa gttgtgaatt
    17401 tcctaaattt tcagtattag aagtaacttt ttctaataat ttttttaatt cagtatggaa
    17461 tgtatggaga tcattataga acatagaagg ttctaagact ttagaatagg caaataaagc
    17521 ctatgcaatt ccacaatatc catgagcaaa gctatagtct aaaatgcttt ctttggctat
    17581 atctctttgt aggaaataat gctaaatttc ccctatacta ttgtggatga aaattttaag
    17641 agtattgtca ttggtaaatt gatagtattt tattaatgat aataggacac cagattttcc
    17701 caatgcaaag tctatttcct ctagagtatc ataattctct attaatttct tagcggagac
    17761 gtttgctaat tgtagatagt attgattttt actgatagaa aataaatgta ggagtaacca
    17821 tagatagcct gcatcaccaa atagcagtga gtattgatat ttatatgtgt gtgtcgttct
    17881 aattgtcact gccttcttga tgatagagag tattttagaa tccagttctg gggggtacaa
    17941 tttatttaga ggaaacagta gtccagctat tccgtgttgg aatgataaat tacttacgaa
    18001 atttccaaat tctgttgaag aagtgatcct tccttcagaa tttacagttt tatctagtaa
    18061 aaatcgtaga acactattac attctgtttt gtaatcatat ttatgttcct ctattttttt
    18121 aattttgact aaattatttg tttgacttaa atttagtaga cattgtaacc aaaatagttg
    18181 ctcatctgtg agctgacctt gagtatgtga tagttctagt aattgaatag gatcctttag
    18241 ttagattcaa tcaatctata acacaacaac ataataatac ttttaggata acttgtcaa
//
LOCUS       DAVKHZ010000042        18183 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000042 DAVKHZ010000000
VERSION     DAVKHZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.042"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..551)
                     /locus_tag="VWW99_001299"
     CDS             complement(<1..551)
                     /locus_tag="VWW99_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9539135.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTA"
     gene            793..1464
                     /gene="sdaAB"
                     /locus_tag="VWW99_001300"
     CDS             793..1464
                     /gene="sdaAB"
                     /locus_tag="VWW99_001300"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9539136.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            1473..2345
                     /gene="sdaAA"
                     /locus_tag="VWW99_001301"
     CDS             1473..2345
                     /gene="sdaAA"
                     /locus_tag="VWW99_001301"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9539137.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            2367..2999
                     /locus_tag="VWW99_001302"
     CDS             2367..2999
                     /locus_tag="VWW99_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9539138.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            3025..3123
                     /locus_tag="VWW99_001303"
                     /pseudo
     CDS             3025..3123
                     /locus_tag="VWW99_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(3485..5335)
                     /locus_tag="VWW99_001304"
     CDS             complement(3485..5335)
                     /locus_tag="VWW99_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9539139.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFHTLSGDELKQAIIAFANQTTHIE"
     gene            complement(5377..6534)
                     /locus_tag="VWW99_001305"
     CDS             complement(5377..6534)
                     /locus_tag="VWW99_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9539140.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLYQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            6592..7758
                     /locus_tag="VWW99_001306"
     CDS             6592..7758
                     /locus_tag="VWW99_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9539141.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            7897..8223
                     /locus_tag="VWW99_001307"
     CDS             7897..8223
                     /locus_tag="VWW99_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9539142.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            8210..8803
                     /locus_tag="VWW99_001308"
     CDS             8210..8803
                     /locus_tag="VWW99_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9539143.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            8796..9707
                     /locus_tag="VWW99_001309"
     CDS             8796..9707
                     /locus_tag="VWW99_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9539144.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            9770..10834
                     /locus_tag="VWW99_001310"
     CDS             9770..10834
                     /locus_tag="VWW99_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539145.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(11012..11872)
                     /locus_tag="VWW99_001311"
     CDS             complement(11012..11872)
                     /locus_tag="VWW99_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9539146.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEMQPILD
                     KDFKTLAQEK"
     gene            complement(12147..13133)
                     /locus_tag="VWW99_001312"
     CDS             complement(12147..13133)
                     /locus_tag="VWW99_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9539147.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(13405..14880)
                     /locus_tag="VWW99_001313"
     CDS             complement(13405..14880)
                     /locus_tag="VWW99_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9539148.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(15138..16061)
                     /locus_tag="VWW99_001314"
     CDS             complement(15138..16061)
                     /locus_tag="VWW99_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9539149.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(16075..17004)
                     /locus_tag="VWW99_001315"
     CDS             complement(16075..17004)
                     /locus_tag="VWW99_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9539150.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(17323..17544)
                     /locus_tag="VWW99_001316"
     CDS             complement(17323..17544)
                     /locus_tag="VWW99_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539151.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSRKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(17498..17758)
                     /locus_tag="VWW99_001317"
     CDS             complement(17498..17758)
                     /locus_tag="VWW99_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539152.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         5401 a         3901 c         3429 g         5452 t
ORIGIN      
        1 gctgtccatg aaccgtaacg tcctgcaacg taggcatctg ctacacgttc ttggttttca
       61 gctgagtagt caccgttcag gtatgaatct gttaattggt aacgtccgat ataacgtcca
      121 tttgtagctg tatagctacc acctgattct ttttgagcga tccattcttt ggcttctgct
      181 tcagatccgc ttacagttga aacaactgtt tcacttacta ctggagtttc tgccactgga
      241 gctgaaacag tttcatcttg agcggctggt gccgcataag tagctggcgc tggtgttgca
      301 acaggcgcta cagggccatc gataaccaac tcttgaccaa cataaatcaa atgaatgtta
      361 tcaatgtggt tgttttctgc caatttttca actgttgtgt tgtgagtttc agcgatttct
      421 gaaagtgtat caccttcttt aacagtgtaa gttgatgatt cttgagcaga tacaaatgat
      481 ggagcaaata ctgcaaacaa ggcagctact cctgcaagag ttgctttaat ctttttagtt
      541 attgatttca tattcgaaaa ttctccttct ttctatagtt ctatcatacc ctttaaatat
      601 taccgtttct tgacactttt atgtagaaat attacaaaat tattttttat ttccctaaat
      661 aaggtgtttt ttgtcataaa agatactatt tttatgctat ttgaggtatt agaaaggttt
      721 tcatccacaa ttaaagccaa gcccctcatt ctttgatata atagagataa gaatttttat
      781 aaggggaaac tgatgaaatc acttcgtttt caatctgtct ttgatatcat cggaccagtt
      841 atgattggcc catctagtag ccataccgct ggtgctgttc gtattgggaa gattgtctct
      901 tccatttttg atgatactcc gacagaagtt gaattccaac tttttaactc atttgccaag
      961 acctatcgtg gtcacggaac agacctagcc cttgttgcag gtattttagg aatggataca
     1021 gatgatcctg aaattccaaa tagtctggaa attgcccaca agcgtggtat caagattgtc
     1081 tggaccattc agaaagacag caatgcccct caccctaaca ccactaaaat tactgttaaa
     1141 aatgcccaca agaccatcag cgtgactggt atttctatcg gtggaggaaa tattcaggta
     1201 accgaactca atggttttgc cgtctctctc aatatgaata caccgactat catcatcgtt
     1261 catcaagata ttccaggtat gattgccctt gttacagagg cgctttcccg ctatggtatc
     1321 aatatcgccc agatgaatgt cactcgtgaa aaagctggtg aaaaagccat tatgattatc
     1381 gaagttgaca gtcgcaactg tgatgaggct atcgaagaaa ttcgaaaaat ccctcatctc
     1441 cacaatgtca atttctttaa ataggaggaa gcatgtttta ttctatcaaa gaattggtcg
     1501 agcaagcaga tctggacttt caaggaaatg tcgcagaact catgattaca acagagtttg
     1561 aattgaccgg tcgcgaacgt gaagaagtct tccttctcat ggaacgcaat ctggaagtta
     1621 tgaaagcctc tgtccaactt ggcctcaatg aaaataaatc tcgtagtggc ctgacaggtg
     1681 gagatgctgc caaattggat cactacatcg aaaacggaaa aactctgtca gattatacga
     1741 ttctctcggc tgcccgaaat gccatcgcag tcaatgaaca caatgccaaa atgggcttgg
     1801 tctgtgccac tccaaccgct ggaagtgctg gctgtctgcc atccgttctc actgctgcta
     1861 ttgaaaaatt agacctcagc cacgagcaac aattggattt cctctttgct gctggtgcct
     1921 ttggactagt catcgcaaac aatgcctcca tctcaggtgc tgagggtggg tgtcaagctg
     1981 aagttggttc agcctctgct atgagtgctg ccgccttgac tctggctgca ggtggaacac
     2041 cttatcaggc cagtcaagct attgcctttg tcattaaaaa tatgctaggc ctcatctgtg
     2101 accctgttgc aggtttggtc gaagttccct gtgtcaaacg taatgccatg ggagctagct
     2161 ttgctttcat cgcagcagac atggccttgg caggtatcga atctaaaatc cctgtggatg
     2221 aagtgatcga tgccatgtac caagtaggag caagcatgcc aactgccttt cgtgaaacag
     2281 ctgaaggtgg actcgctacc acccctactg gtcgtcgcct ccaaaaagaa attttcggag
     2341 aataagctta tcaaatagga gaaaccatga cctctatcac agcgattttt ttcgatttgg
     2401 atggaaccct cgttgatagt tctatcggga ttcacaatgc ctttacctat acctttaaag
     2461 agctgggggt gcctagccct gatgccaaaa ctattcgtgg ttttatggga ccacctctcg
     2521 aaagtagttt tgcgacctgc ctgtccaaag accaaatttc tgaagccgtg cagatatatc
     2581 gttcttacta taaggcaaaa ggcatctatg aagctcaact ctttcctcag attatagact
     2641 tgcttgagga gttatcgagc agttatccac tctacatcac cacgacaaag gatacatcta
     2701 ctgctcaaga catggcaaaa aacttggaaa tccatcattt ctttgatggc atttatggtt
     2761 ctagccctga agcaccccat aaggcagatg tcattcacca agccttgcag acacatcaac
     2821 tagcaccaga acaagccatc atcatcggag ataccaagtt tgatatgctg ggagctcgag
     2881 aaacaggtat tcagaaattg gccatcactt ggggatttgg agagcaagca gatctactaa
     2941 actatcaacc tgattatatc gctcacaaac ccttagaagt tttggcgtat tttcaatagc
     3001 atagactggg taacaattcc atttcagaag aaaatgaagt aatactcaat gaaaatcaaa
     3061 gagcaaacta ggaaactagc tgcgggctgc tcaaaacact gttttaaggt tgtagatgga
     3121 actgacgaag tcagctcaaa acactgtttt aaggttgtgg atggaactga cgaagtcagc
     3181 tcaaaacact gttttaaggt tgtggatgga actgacgaag tcagctcaaa acactgtttt
     3241 gaggttgtgg atagaactga cgaagtcagc tcaaaacact gttttaaggt tgcagataga
     3301 actgacgaag tcagctcaaa acactgtttt aaggttgcag atagaactga cgaagtcagt
     3361 aacatataca tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtataa
     3421 gtctctacat aataaaacgc atattaccaa ggttgtgtaa cttgatacta tgcgtttttc
     3481 ttttttattc aatgtgggtt gtttgattag caaaggcgat aatagcttgc ttcaactcat
     3541 ctccactgag agtgtggaac tctccaatct tttgattgat ggattctaaa ggcatgacat
     3601 taaccttacc aacgaaaatc ttatccataa cttgattatc aacgagttcg gttgatacca
     3661 agagttcttc gtagagtttt tcacctgggc ggattccaac ttcaacgatt ggaatttcac
     3721 tttcagtgtg gccacttaga agcaccatct tcttggccaa gtcataaatc ttgactggtt
     3781 tgcccatatc aaggataaag acttccccat ctttggcata agcaccagca tggataacca
     3841 gacggctagc ttctggaatg gtcataaagt aacgggtcat gcggaagtct gtcaccgtta
     3901 caggcccacc ttcagcaatc tgacgttcaa agactggaat gacactacca cggctaccaa
     3961 gaacattccc aaaacgaact gcacagtagg ttgattggct acgttggtta aagccagtga
     4021 caatcaactc cgccacgcgc ttggttgctc ccataacatt tggtggatta actgccttat
     4081 ctgtcgaaat cataaccatc ttagacactt tagcttcatc aacagcctta gcaacattgt
     4141 aagttccacg gatattgttt ttgaaggctt cttttggatt gcgctccatc ataggaacat
     4201 gcttgtgggc tgccgcatga taaacaatag caggtttgta ctgctcaaag acttgcaaca
     4261 aacgatcata gtcttgaatg tccgcaatca caggtacata atcaatccct tggaacttac
     4321 gaatcaattc atgataaaca aggtagattg agttttcccc atgaccgagc aagacaatgc
     4381 gttcaggatt gaagcgacta acttgacgac agatttcaga accgattgaa cctccagctc
     4441 ctgtgactaa gatggtctta cctgtcagtt ctgcacccag acgcgattcg tcaagacgga
     4501 tttcctgacg acccaaaagg tccgtaatat caattttttg gaagccagta cctgcttggt
     4561 gaaggccctg aacaacagtt tcaaccttag gcatcttgta acatttgaca cccagcttat
     4621 tacacatctg caagatacgc tcatattctg acggatccag cgacggaatc gcaacgatga
     4681 cacgctcgat ttgatggcgt ttggctaatt caggcagatt gtcataagag cccaaaacag
     4741 gaataccacc aagtttttga ccctttttct tagaatcctt atccaaaata ccgaccagtt
     4801 ctaattcact ggttggatgt tggtaactat ccataaaaag agccccacca tcaccggcac
     4861 caatcaagaa ggtccgacgg tgttctccat caccactacc ttttttgcgt ctggagtaga
     4921 ttaactgcca agtaatccgt ggcaataaaa tcaagaaggt actcaacaag ataaagagaa
     4981 tgataaaacg gatggagaag agtggcaaga aggcataaca gatactatat gacaagacac
     5041 tgctagcagt cacaccaaaa aagattttca tgaaatccgt aatcttgctg taacgactaa
     5101 tgctcgcgtt caacccccaa aaaccaatca tcaattgata gaacaggaag gccaaactcg
     5161 tatagataat gtagtcaaca ggtgctggat taatcagccc ataaaataaa atataagata
     5221 caattatgga aaccaccata ctgaaaatat caaatattcc ccagaaaacc tgcttttgtt
     5281 gtttatttaa aatttccacc agatcaatca cataatctgt tagtttttta ttcatagaaa
     5341 tctactcctc cttaaaagag ttttactata ttgttattat agcatatttt tatttatttg
     5401 gcttatttta tccaaataaa ttattgttct cttgtactcc ttcttccatt taaatcctct
     5461 aattaaacct acaatcgtcc caaagccata agcaaagtga atggaaaata aaagaaaggg
     5521 catcacaatt agaaatccat ttttatgttt taataaagtc agcaaagtga gtagtgacaa
     5581 aagtagaaaa taggcaccta atagtaaagt tatgaatacg aatgtgatcg gtaacaatgc
     5641 tagactaaac acaagactca aaacaaataa acaaggaaca tagtgaaata atgataaaca
     5701 cttaaactga acatgacttg tcaagccaat ccacaaacca tttgaatact tttgatacag
     5761 cattttcttg aatgttggtc gaatatactg ataagataga atacttgggc tatagcgaat
     5821 tttataacca tgttctcgaa ttctataatg aatatcatta tcttcagttc ggccaagttg
     5881 ctcatttact aaaccaacct tctggaaaac ctctcgttta tacatcccat gaaaaataga
     5941 agaaacatat ctatcttcgg aactatttcg ataattggca atgctactgc caaacatatt
     6001 ttcctcaaca agatgcaagg tctctgccca ttttcctttt ccttcgacaa tcgtcggtct
     6061 aggcccccca cagacaaatt caccttgttg aataatagcc acattgttca ttacaaaagt
     6121 ctcagtaact tttgaatgag catcaatttt taaaataagg tcccctacag aatgtttaac
     6181 tcccaggtta aaaccactag cttgattttt cttaggattg ttatacaatc taattgagtt
     6241 aaactctgta tcttccttta taaattgctg aatgatagct gtggtcccat ctgtggacat
     6301 agcatttata aatagaattt caatattctc tttaggataa gtttgatttt ttaagtcttc
     6361 aattagacca ggcagatatt tttcttcatt gtatgcactg attacaatgg atacgagaag
     6421 actttcaaca ttttgagtca ctactactct cctgaatttt tccttccacc attctatcat
     6481 aattttcaag actttcccat ttttatcttg aaagccagaa accttacttg acattatagt
     6541 gaaaaagcct taaaataagc tgttattaaa atcagctaga agaggtatta tatgaaaaag
     6601 caatcactct tttttgttcc aggtattatc ctgattggtg tttccttgcg aactcctttc
     6661 actgttttac ccattatttt gggaaatatt tcgcaaggtc tggaggtaga agttagttcg
     6721 cttggtgtct tgaccagcct gcctctcctt atgtttaccc tcttttcacc attttctacc
     6781 caactggctc agaaaatcgg cttggagcat ctcttcacct acagcctctt cttcttgacc
     6841 atcggctcac tcattcgact aatcaatctg cccctgctct atctaggaac cttgatggtt
     6901 ggggcaagtg tcgcagtcat caatgtcctg cttcctagtc ttatccaagc caatcaacca
     6961 aagaaaattg gttttctgac caccttatat gtaacgtcta tggggattgc aacggctctg
     7021 gcttcctatc tagctgtgcc cattacacaa gccagttctt ggaaaggact tatcctcctc
     7081 ctcacgttac tctgtctagc aacttttttg gtctggctcc caaatcaccg ctataatcat
     7141 agactagctc cacaaaccaa acaaaaaagt caaataaagg tcatgcgtaa taaacaggtt
     7201 tgggcaatta ttatcttttc aggttttcaa tccttgatct tttacaccgc catgacctgg
     7261 ttacctacca tgtctatcca tgcaggtcta tccagtcacg aagctggctt gctgacttct
     7321 atcttatctc tgattagcat tcctttttca atgaccatcc caagcctgac aaccagttta
     7381 tctactcgca accgtcagct catgctcact ctggtttcac tagctggtgt ggtcggcatt
     7441 tccatgctct ttttcccaat caataatttc atttactggc ttgccatcca tctcctcatc
     7501 ggaaccgcaa ccagcgccct cttcccttat ctcatggtca acttttcact caagacaagc
     7561 gcccctgaaa agacagccca attgtccggc ctatctcaaa caggaggcta tatcctagca
     7621 gcctttgggc caaccctctt tggttacagt tttgacctat tccactcttg ggtaccagct
     7681 gtagctgccc tcttgctcat cgatatcctg atgactgtgg ccctctttac agtggacaga
     7741 gcggataaga tcctttaaac ttctagtttt agctagaagt tttttatata taaaaatttt
     7801 acacatttct cttgacaggg ctttctttta gatgtacaat gtatgtgtag aaaaattata
     7861 tataaaaatc ttacacatta gaaaaggagg tttcccatgt actttccaac atcctctgcc
     7921 ttgattgaat ttctcatctt ggctgtactg gagcagggtg attcttatgg ttatgagatt
     7981 agccaaacca ttaagctgat cgctaatatc aaagaatcca cactctatcc cattctcaaa
     8041 aaattggaag gcaatagctt tctgacaacc tattctagag agttccaagg tcgcatgcgc
     8101 aaatactact ccttgacaaa cggtggtata gagcagctct tgaccctaaa agatgaatgg
     8161 acactctata cagacaccat caatggcatc atagaaggga gtatccgcca tgacaagaac
     8221 tgaatacctg actcagctag aactctatct caagaaacta cctgaagctg accgtatcga
     8281 agccatggac tatttcagag agctctttga cgatgctgga gtcgaaggag aagaagaact
     8341 catcgctagt ttgggaactc ccaaagaagt ggcccacgaa gttctatcca atcttctcga
     8401 taaaaaaatc aatgaagcac ccgctcaaaa aaataaccga caaattttac atatcgcctt
     8461 gttagccctc cttgcagcac ctatcggcat tcctctggga atcgccatcc tcgtgaccct
     8521 gttcgcaatc cttgtagccg ctttgactgt cattctggct ttctttgcag tttccatact
     8581 gggtatcatc ggcggattcc tatttttagt tgaaagtttc actgtcctcg cccaagccaa
     8641 atcagccttt atcttgattt ttggttctgg tttactggct atcggtgctt cttcgctagt
     8701 tttacttggc atttcctatg tagctcgctt cttcggtcta ctcattgttc gtctggtaca
     8761 atttgttctt aaaaaaggaa agagaggtaa tcagcatgcg taaatggaca aaaggatttc
     8821 tcatctttgg tgtggtgact accgttatcg gctttattct gctttttgta ggtatccaat
     8881 ctgacgggat taagagccta ctttccatgt ccaaagaacc tgtctatgat agccgtacgg
     8941 aaaagctaac ctttggcaag gaagtcgaaa acctagaaat tactctccac caacacacgc
     9001 tcaccatcac agactctttc gatgatcaaa tccacatttc ttaccatcca tctctttctg
     9061 ctcaccatga tcttatcacc aatcagaacg atagaactct gagtctcact gataagaaac
     9121 tgtctgaaac tccgtttctc tcttctggaa ttggtgggat tcttcatatc gcaagtagct
     9181 actctagtcg ttttgaagaa gttattctcc gactaccaaa agggagaact ctaaaaggga
     9241 tcaacatctc agccaatcgc ggacaaacca ccatcataaa tgctagcctt gaaaatgcga
     9301 ccctcaatac aaacagctat atcctccgaa ttgaaggaag tcgtatcaaa aacagtaaac
     9361 tcacaacgcc caatatcgtt aatatctttg atacagttct tacagatagt cagctagagt
     9421 caacagagaa tcacttccac gctgaaaata tccaagtcca tggtaaggtt gaactgactg
     9481 ccaaagatta tctcagaatc atcctagacc agaaagaaag ccaacgaatt aactgggaca
     9541 tctcaagcaa ctatggttct atcttccaat tcacaagaga aaagcctgaa tcaagaggta
     9601 cggaattaag caacccttac aaaactgaaa aaaccgatgt caaggatcaa ctcattgcga
     9661 gatctgatga taatattgat ctaatatcca caccaagcag acgttgacaa aaacgcttac
     9721 atctgctact atggaagcat atttatctaa ctaaaaagga ggttctacca tgaaacaaga
     9781 atggtttgaa agtaatgatt ttgtaaaaac aacaagcaag aacaagcctg aagagcaagc
     9841 tcaagaggtt gcagacaagg ctgaagaaac gatagccgat ctcgatacac caattggaaa
     9901 aaatactcaa gtagaggagg aagtctctca agctgaagtc gaactggaaa gccagcaaga
     9961 agagaaaatt gaaactcctg aaggcggtga agcgagaaca gaaatagaag aaaagaaggc
    10021 atctaattct actgaagaag agccagacct ttctaaagaa acagaaaaag tcactatagc
    10081 tgaagagagc caagaagctc ttcctcagca aaaagcaacc acgaaagagc cacttcttat
    10141 cagtaaatct ttagaaagtc cttatatccc cgaccaagct ccaaaatcta gggataaatg
    10201 gaaagagcaa gtgcttgatt tttggtcttg gctagtggaa gcgatcaaat ctcctacaag
    10261 taagttggaa acaagtatca cacacagtta cacagccttt ctcttgctca ttctgttttc
    10321 tgcatcttcc tttttcttta gtatctatca catcaaacat gcttactatg gacatatagc
    10381 aagcattaac agtcgcttcc ctgagcagct agctccttta actctttttt ctatcatctc
    10441 tatcctagta gcgacaacac tcttcttctt ttcattcctc ttgggtagtt tcgttgtgag
    10501 acgatttatc caccaggaaa aggactggac gctagacaag gttctccaac aatatagtca
    10561 actcttggca attccaatct tcctcactgc tattgctagt ttctttgctt tctttgatag
    10621 cctacgattt acagccctct tgtgtgtgat tagcattgga atcattctgc ttgccagtct
    10681 ccatatcatt acaagaccaa gtcaagcaag tgaaaccgac tccttctatc aattattctt
    10741 gtctgtcctt gtgaacggag ttattatcct cctcttcttt gtagctgaag tggcactgat
    10801 tggagattat cttcgtatct tggcctttct ttaaacttca tactcttcga aaatcaaatt
    10861 caaaccacgt cagcttcgcc ttaccgtact caagtacagc ctgcggctag cttcctagtt
    10921 tgctctttga ttttcattga gtagcaatcc tgtcatcctt ggcaggattt tttctatgtc
    10981 aaaaaagcaa gtcgattgac ctgcttctgc tttacttttc ttgtgctaat gttttaaaat
    11041 ctttgtctaa tatgggttgc atttctattt gattggcgtc tagttggtgg taaaaagatg
    11101 ggggtaatac ctctaagaat tttgcatagt ccaagcgggc aatggcttca tagtcttctg
    11161 gtttagagcc gtctcctgtg atttgaacca agtcaatctc ccactggact tccttatcca
    11221 ccaattgctc aatataggct gcaggaacaa acggttctct cggtaaaact gtcttgactc
    11281 cttgagctag atggtaaacg ccatgcactt ttccttctcc atcctgatag aaggaaactc
    11341 tcgcaaagta ggcatctgtc tcttgaaccg cacgcacacg cgcttcaaac tgagccaagc
    11401 catctgcctc taaaaagctt ttcaagttct cacgactaaa gaaaatagga ttaaaaattc
    11461 cttggcaaat cgtaaaacga gctacagggt ctgctagata ggcttgaata cttgcttgga
    11521 aataagcttc aaaatcaaaa ccatctagcc cttcaatatc agtccctgta taagcaagca
    11581 aagcatctaa aaatgacttg ataatctgcc agtctgtctt tgttagtagg tcaggaagat
    11641 cgacacggta agccttttga ccatcagcat aactgacctt aaaaagaaat tgtgattgcc
    11701 ccactatcgc acactcaata tactcgagac gattaagagg ctgacggaga tagactacat
    11761 catagctatg tgactccaaa ccgtctagca aggccaagat cgacttggca ttcaaaatct
    11821 cttgttctcc taaaatgctc tgtttatttg gaataaaaaa tgttttcgcc ataactggac
    11881 tcctttgaaa tcgtttacat acagtatacc ttatttttct gaaagataca aaaacaaact
    11941 ttatattctt cgaaaatctc ttcaaaccgc gtcagcttcg ccttgccgta gatatatgta
    12001 actgacttcg tcagtcttat ctacaacctc aaagcagtgc tttgagcatc ctgcagctag
    12061 cttcctagtt tgctctttga ttttcattga gtattagatt tttgagtaca aagcatagaa
    12121 aaacagcccc tgaggactgt ttgatcttat acagtagctg cctgatccaa gctttcaccg
    12181 atagcggcta ggcgctcaat cacttcagct tgtgtcaatt cattttttga aacatagcgg
    12241 ttacgtgggt gaacacggca ctcgtgtgag catccacgaa ggtacttgtc ttcattttct
    12301 tctgatgtca agatacgacg gttacagaag ggatttccac agttgacata acgttcacat
    12361 ggtgttccat caaaccagtc tttccctacg atggttgggt tgacatggtt gacatcaacg
    12421 gcaatacgct cgtcaaagac gtacattttc ccatcccaaa gctcaccttg aacttctggg
    12481 tctttaccgt aagttgcgat tcctccgtgc aattggccga catctttgta gccttcacgg
    12541 accatccagc ctgagaattt ctcacagcga acgccacctg tacagtaaac cacgacacgc
    12601 ttgtccatga atttttcctt gttatcacgg acccattgtg gtaactcacg gaagttgcga
    12661 atatctgggc gaatagcccc acggaaatgt cctaggtcgt actcataatc gttacgtgtg
    12721 tcaaggacaa cggtatcttt atcaagaagc gcttctttga actcttttgg agacaagtaa
    12781 gcacctgttg tttcaagtgg gttgatgtca ttgtcaaagt cgttgtcttc caaaccaagg
    12841 tggacaattt ctttcttgta gcgaacaaac atcttcttga aggcttgttc attttcttcg
    12901 tcaatcttga accagagttc ttccattcct ggaaggctgt gaacgtagtc catgtatttt
    12961 tgagttgttt catagtcacc tgaaactgtt ccgttaattc cctcgtcagc gactaggata
    13021 cggcctttaa ggccgattga tttacagaaa gccaagtggt ctgcagcaaa ttgctctgca
    13081 ttttcaattg gagtataaag gtagtaaagt aagacacgaa tatcttttgc cataagattt
    13141 gttctctttt ctattcttaa attttctaaa ttttttattt atctacacta gtatacctgc
    13201 ttgagaaaac gaatgcaatt tatttgactg gaaattgtag agggttctcg aaatttcctg
    13261 aatggttaaa ataaggacaa gagaaaacat ggatatctat atccttgtgc caaaaaaacc
    13321 actgccctcc ccagaccaac ctgaggaaag cagtgattct tattttagga gttaggaatg
    13381 aatacacgaa atcaatttag ctgattattt tttgtttttc aagaattcat cgtattgttt
    13441 ttgcatttcg ttcaatactt tttcgtaggc accttcagat ttcaattttt ccatcaattc
    13501 tggaatcgct ttatctgggt ctacagtacc agtgttgata gctgtatcaa attgttgcat
    13561 tgtgttagca atagctgaga tttcagattt cacattgtca gtattgaaga taaatccaag
    13621 cgctggagat tctttagctt ctgccaattc tttcttagaa ttttcgattt gttggtctgt
    13681 aacgttttcg ttgatgtaaa ggatccagtt gttaccagtg ttccatccac ccatgtgagt
    13741 gtttcctttg tagccatcaa gaacgcgaac acggttttct ttaccttcaa ttttttccca
    13801 gttcttgcct tctggaccgt aaacaagacc gttcaagagt tctggattcg tattcaagag
    13861 gttcaagatt tccattgatt tttctttgtt cttagagttg tttgagatga caaagttagc
    13921 aacttgtgtt gtttggtttt tcttgatgaa gttagtaatt ggtttgattt ggatatcttt
    13981 gttggcaaca cgtgaaagca agctgttacc gtagtcagct ggtcctactg tttcttcacg
    14041 aacgaaccaa gtatcttgtt gaaggtcaaa ggaagtatcg cttgttgcga cgtcttttgg
    14101 aatgtagcca gcttcataga atttgtgaag agtcttcaag tgttctttga aacgaggcac
    14161 ttcgtaacgg tttacaactt tagtagtatc gccttcaagg tcgataacga atggaagacc
    14221 gtttgctact gggtagtcaa agttatcaga tgggatgaaa actttaccaa tagcaaatgg
    14281 tactacgtct ggagcttttt ctttgatttg tttcaagact ggctcaagag tttcgtaaga
    14341 agtaacacct gaaatatcga taccatattt agcaaggaga gttccgttga aggcaaagtt
    14401 ttgagatgat gcaacgttgg ctgcaactgg aacagcgtaa atcttaccat ttacagtatt
    14461 acccttgatg taagctgggt caagtgcttt gtaaaggtct ttaccttctt ttttgtacaa
    14521 ttctgtcaag tcagcgtaag cacctttttg agcatttaca atatagttat ctgcaaaggc
    14581 aatatcatag ttttcaccag atgatgtgat aactgacatt ttcttaccat agtcacccca
    14641 gccaaggtat tggatatcca atttggcacc aactttttct tcgatgattt tgttggcatt
    14701 tgctaacaat tcatccaagt tgtctggttt gtcaccgatt tggtacattt tgataacagg
    14761 tttgtcacct gaatcagcag cttttttgct gttacctgtc aaatttccac aagcagcaag
    14821 acctgcagcc agagcgacta cactagcaga tgcaaaagca tattttttcc agtttttcat
    14881 gataaaaact ccttttttta tttttaaact tataaacaat gtaatgatct tatactcaat
    14941 gaaaatcaaa gagcaaacta gaaaactagc cgcaggctgc tcaaagcact gctttgaggt
    15001 tgtagataag actgacgaag tcagttacat atatctacgg caaggcgacg ttgacgcggt
    15061 ttgaatttga ttttcgaaga gtattaactt cacacaaggg aagttgggaa ctgagaaatg
    15121 ttatttctca ataagcacta ttctttcaca ccaccgatag tcaaaccttt tacaaagtag
    15181 cgttggaaga atggatacaa aatcgcgatt ggaagggttg caaccacaac catggccata
    15241 cgacctgttt ctttcggtag agcaactccc agttgaccag tcaagccgac cgctttggca
    15301 atgtagtcca tattttgttg gatttgcatg agcaaatatt gcaatggata caagttgtca
    15361 ctcttgatgt aaagaagggc gttgaaccag tcattccaga aaccaagagc tgtcaaaagc
    15421 gtgatggttg cgatacctgg tagtgacaat ggcaaacaga tttggaagaa aatccgggcc
    15481 tcactggcac catcgatacg agccgattcg agaatggctt ctggaatggt cttcttgaag
    15541 aaggaacgca tcaagatgat attgaatggt gagagaagca ttggaacaat caaggcccaa
    15601 acagtgtcac caagctgaag tacacgggtc accatgatat aacctggtac caaaccagcg
    15661 ttgaacaaca tactgagaag gacgaagatg gtaaaaaatc tgcgatactt aaaggttgtc
    15721 cgtgaaatag cgtaggcata ggttgttgtg ataaagacat ttgtcaatgt cccaactacg
    15781 gttacaaaga cagagatgaa gagggcttgt aggattttat ccttaaactg tgccaaaaac
    15841 tcaaaaccgt ctaagccaaa ttgggatggg aagaagctat agccgtattg gaggaggctt
    15901 ttctcgtctg tcactgaaat aatgataacg aatacaaaag gtaggataca agagagggca
    15961 atcaaacccg aaatgatact gaagaagata tctgctttct tactgaagga gtgaatgccg
    16021 acattatcaa ttttttcttt tttaattttc ttttttgcca tattctcctc ctttctagaa
    16081 caaagctgag tttggatcga ctcgtcttgc aagcaagttt gataggataa ccagaatcaa
    16141 accaacaacg gattggtaaa gaccggctgc tgcagccata ccgatatctg ctgtctgagt
    16201 caaaccatta aaaacatata cgtccaaaac gttggttaca ttgtaaagct gaccagcatt
    16261 gtgtgggatt tgatagaaga gaccgaagtc tgcgcggaag atatttccga ctgcaaggat
    16321 ggtcaataca gttacaagcg gagtcaactg aggaatggtt acgttgcgaa tacgttgcca
    16381 cttgctagct ccgtccactg tcgctgcttc gtagtaggtt ggatcaattc ccatgatcgt
    16441 cgcatagtac atgacactgc tatatccaaa gcctttccaa atacctagga aaagtaggag
    16501 atagggccag atgcccaggt cagcgtagaa attgacttct ttgagaccaa gactttccaa
    16561 tagatgattg aacacccctt tatcaatatt taggaaggca tctgtaaaga aactaatgat
    16621 aacccaagac aagaagtaag ggaacaacat agaagtttga aaaatcttca ccattctctt
    16681 agaacggagc tcgctgagga taatggcaat ccctacagat acaactaaac ctagaaagat
    16741 aaagccaaga ttgtagagga cagtatttcg tgtgataata aaggcgtctc ttgaactaaa
    16801 taagaatctg aaattatcga gtccgaccca tttactattt atgatactat ctatgaaacc
    16861 attactggtc atgtggtagt ctttgaaggc aaccacgttc ccaaatactg gaatgtaaaa
    16921 gaatagaatc aaccagagtg cccctggcaa aaccatcaag agaaagatcc agttgtctct
    16981 caatgttttt gaaaactttt tcataatttc ctcccttttt attttgatat ccatctaaaa
    17041 attctttttt agacttttga taacgattac attattagta tactcctatt tgcaggttag
    17101 gttaaactcc taattataga aaaaactcca caaattatgt agcagattta aaactttatc
    17161 accactatca aacaaatgtc ctaaatcaat tgtttatttt atctctatta gcccagtgat
    17221 ggcgtcactc tgttataagc atccaacaac ggggtatact gaaaaatctc cagactaggg
    17281 aactcagaga tagttcctaa tctggagatt tttaatatgt tattaggcgt ttgctttcaa
    17341 cttagcaata acctctttaa gattatcaat cacctctgct tcagtatgct cagagccttt
    17401 ttcatctgcc aagaacaaaa ctgctttttg aagttctttt cgagagtttt caaggacatc
    17461 cttatctact gtttcaaggt ttgagtcttt aagaagttta cttaattcct tggctaattt
    17521 cttgagtttg atttgcagac tcatcttctc ctgctgtttc tttgcccgct gtttgtcctc
    17581 catccttagt tgctgactgg ctttccttaa tggactctag ggaagcaatg gcatctttga
    17641 ctgtttgcaa gatatcacgt aaaccttgct ctgtcaaact atcatctgca agagctttct
    17701 tagcctctgc caaaaccaga cgtgctgaat ctgtggtagg attcgataca cctgtcaatg
    17761 atctcaaaag attttctaag gtttgagtct gcttactaat actagactaa aatcaaaaag
    17821 cattacataa cagtgatatg aaatcaacta aagaagaaat ccaaatcatc aaaacacttt
    17881 taaaagactc tcgtacagct aaatatcata aacgccttca aatcgttcta tagtaaaatg
    17941 aaacaagaac aggacaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
    18001 gtagatgtgt actattctag tttcaatcca ttatatttcg tctgatgggc aaatcttaat
    18061 aaagagatta tagagctttt atagtgattt gaaataagat gtgaacaact ctatcaggaa
    18121 agtcaaatta atttatagaa atattttagc agccaaggtg tactgttata aattcaatac
    18181 act
//
LOCUS       DAVKHZ010000043        17954 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000043 DAVKHZ010000000
VERSION     DAVKHZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.043"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VWW99_001318"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VWW99_001318"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9539153.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VWW99_001319"
     CDS             1377..1886
                     /locus_tag="VWW99_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539154.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4365
                     /locus_tag="VWW99_001320"
     CDS             1963..4365
                     /locus_tag="VWW99_001320"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9539155.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4433..5434)
                     /locus_tag="VWW99_001321"
     CDS             complement(4433..5434)
                     /locus_tag="VWW99_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9539156.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5590..6036
                     /locus_tag="VWW99_001322"
                     /pseudo
     CDS             5590..6036
                     /locus_tag="VWW99_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6162..6428
                     /locus_tag="VWW99_001323"
     CDS             6162..6428
                     /locus_tag="VWW99_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539157.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6689..8938
                     /gene="metE"
                     /locus_tag="VWW99_001324"
     CDS             6689..8938
                     /gene="metE"
                     /locus_tag="VWW99_001324"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9539158.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9002..9868
                     /gene="metF"
                     /locus_tag="VWW99_001325"
     CDS             9002..9868
                     /gene="metF"
                     /locus_tag="VWW99_001325"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9539159.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10553..12766
                     /gene="pnp"
                     /locus_tag="VWW99_001326"
     CDS             10553..12766
                     /gene="pnp"
                     /locus_tag="VWW99_001326"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9539160.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12782..13399
                     /gene="cysE"
                     /locus_tag="VWW99_001327"
     CDS             12782..13399
                     /gene="cysE"
                     /locus_tag="VWW99_001327"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9539161.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13411..14295
                     /locus_tag="VWW99_001328"
     CDS             13411..14295
                     /locus_tag="VWW99_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539162.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEERESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14382..15725
                     /locus_tag="VWW99_001329"
     CDS             14382..15725
                     /locus_tag="VWW99_001329"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9539163.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15718..16104
                     /locus_tag="VWW99_001330"
     CDS             15718..16104
                     /locus_tag="VWW99_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9539164.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16108..16992
                     /locus_tag="VWW99_001331"
     CDS             16108..16992
                     /locus_tag="VWW99_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9539165.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17129..17584
                     /locus_tag="VWW99_001332"
     CDS             17129..17584
                     /locus_tag="VWW99_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539166.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            17581..17727
                     /locus_tag="VWW99_001333"
     CDS             17581..17727
                     /locus_tag="VWW99_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539167.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
BASE COUNT         5394 a         3470 c         4067 g         5023 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagtg gtaattgaat agaaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctgac tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga cagctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggagccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggtt ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataatcgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag aggattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttat atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacaag agttggctga aaaaatgtca
     2041 actacaggaa tcgaggtaga gggtgtcgaa tcaccagctg ctggtctctc aaaaattgtc
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc acctccatgt ttgtcaggtt
     2161 aacgttggcg aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgctgggatc
     2221 aaggtcatgg tggctcttcc aggagctcgt atcgctgata actacaaaat caaaaaagga
     2281 aaaatccgtg gtttggagtc acttggaatg atctgttcac ttggtgaatt gggaatttct
     2341 gactcagttg tgcctaagga attcgcagat ggcatccaaa tcttgcctga agatgccgtg
     2401 ccaggtgagg aagtcttttc ttacctagac ttggatgatg aaatcatcga actttccatc
     2461 acaccaaacc gtgcagatgc cctttctatg tgtggagtgg ctcacgaagt ggcagccatc
     2521 tatgacaagg cagtcaactt taaagaattt actctaacag aaactaatga agctgcggca
     2581 gatgcccttt ctgtcagcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     2641 gacaatgtga ccatcgcacc aagtccacaa tggttgcaaa atcttctcat gaacgaagga
     2701 atccgtccca tcaataacgt agtggacgtg accaactaca tcctgctcta ttttggtcaa
     2761 ccaatgcatg cctttgactt ggataacttt gaagggactg acatccgtgt gcgtgaagcg
     2821 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     2881 ctagtcatca ctgtcgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     2941 acagaaatct ctgaaaaatc tagtcgtgtt gtccttgaag ctgctgtttt caatggcaaa
     3001 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     3061 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     3121 gaacttgcag gtgcgacggt gcgtaagggc atcgtttcag cgggtgagct tgatacttca
     3181 gatgtagaag tttcttcaac ccttgctgat gttaatcgtg tcctcggaac tgagctgtct
     3241 tatgctgatg tagaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     3301 agctttacag tcagagtccc acgtcgtcgt tgggatatca caatcgaagc tgacctcttt
     3361 gaagaaattg ctcgtatcta tggttatgac cgcttgccaa ctagtctacc aaaagacgat
     3421 ggtacagcag gtgaattgac agccacacaa aaactccgcc gtcaagttcg tactattgct
     3481 gaaggagcag gtttgacaga aatcatcacc tatactctaa caactcctga aaaagcagtt
     3541 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     3601 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     3661 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     3721 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     3781 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taagggaatc
     3841 cttgaagccc tatttactcg tttgggactc caagtaacct atacagcaac atctgaaatc
     3901 gctagccttc atccaggtcg tacagccgtg atttcattcg gtgaccaagt tcttggtttc
     3961 cttggccaag tgcatccagt cactgccaag gcttacgata ttccagaaac gtatgtggct
     4021 gagcttaacc tttcagctat cgaagctgcg cttcagccag cgactccatt tgtagaaatc
     4081 accaaattcc cggcagtcag ccgtgacgtt gcccttctcc tcaaggcaga agtgactcat
     4141 caagaagttg tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     4201 tttgacgtct tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     4261 accttccaaa atccagaaga tagcttaacg gacgaagaag tcgcacgcta tatggaaaaa
     4321 atccaagcat cgctcgaaga aaaagtcaat gcagaagtgc gttaaacgat agtatgatac
     4381 aaattccccc tctagtttat gaacactaga ggggtgattt tatataaaat aattaaaatg
     4441 tgatttctgt aaaaatacat ctatgatcag agttttgaaa acttacaatg tctaaatctt
     4501 taacatagta gtggttttta ggcaataaaa tatgatctat tgttgcatta aaaagttttg
     4561 gactttggct attccaagtt cctctttcaa aaggtggcag tacatttaat gcgtccctat
     4621 gagagcttat ttttgcaagt gctccatgac gcatagttgc attaaaatca cctgcaataa
     4681 tagcctttgg atattttgaa gccaattgat tatgaatgat gtttaagtct tgcttccaga
     4741 tttccattaa acctggcaga ggaggagcag tatgcaaggc aatgatatct ggtatatttt
     4801 gttttctcga atgtaataca attgtcccga accgtgttgt atgaaaagtt ttagcttctg
     4861 tatagaaacc ataacttttc ttgacaatca cagtcacagg agctattcca ctattggtag
     4921 gtggagaagt gaaaatatca tagttggcca tagaaagtcc aacttgatga aacaatagtt
     4981 tgattctctg gttttcttgc tcacctctga tattggtagc tagttcaggg aatatagcca
     5041 tatcggcgtc aaaatggcta aaaattcgct caatatgttg tgcttctatt tgattagcga
     5101 cgttccaagt tactaatttt aaggttttag tcttatttga tgcagaagaa agggttttat
     5161 ctgttccgaa taatagtaat atgagcgata gtaagagcaa aatgccagag taaaaccgta
     5221 tttgtttatt tttcttgtaa aaagataggg aaaaaaatac gaaacttaaa atagcagcca
     5281 tagttgcagt agctaagggg aaggcataaa tctgtgccaa gataggttgg gtagatagcc
     5341 aaatgttaag attagaagat gggaaaaaag aataagttat aatagggata gaaaagagaa
     5401 tgaccgaaag ggcagagaga aatgttttaa tcattgtgtg agtacctcaa tttgcaatgg
     5461 ttgctcggag ctaatgtgac tttgtgactt tacttgattg atattctttt tcttgtcttc
     5521 ctcatagatt aagtacaacc caagtaaaaa aagtccaatt taagattgaa atgattaaaa
     5581 ggcacagaaa tgcaagacaa ttatactaca aaaggcaaac atttgacaat cgatagccgt
     5641 cgcttaatcg aacgatggaa aaaagaagga aaatcaaata gagaaattgc ctctctactt
     5701 ggaaaagctc ctcaaactat ccatactgaa atcaagtatg ggacagtccg acaatgtctt
     5761 ggaaaagggc gcttcaaaga gatttattct gccgactacg ctcaacagtc ttatgaaaat
     5821 aatcgcaagc actcggtcaa gaaatcaagc ttgactaaga aactaaagga aaagattctc
     5881 cactatcata accaaaaatt ttcgcctgaa atgatggtga tggctaaagg ggttaacgtg
     5941 ggaatttcaa ccatttacta ttggattcat cgtggaaaat tggggttaag caagcaggat
     6001 ctgctttatc ctagaaaagg aaaagcgctt aagaaatagg ctagcaccaa ctttaaaccc
     6061 gctggtcaat ctatcgaatc ataattcgct cattttttta aaaatacata gacgtaaaaa
     6121 aaagagggtt aagtgatgaa gaatagtgct attgggagta attggaagga tgtccatctg
     6181 agctctttag caaggaggaa atccttgaaa gtgatatgcg agtggctatc atgagcgagt
     6241 tgattgaggc taggtatgaa caaggaatca gtcagaaaaa gctggaagaa gtcagtggaa
     6301 taagccagcc tgtcatagct aggatggaga caggagagag cagtcttcag ttggatacgg
     6361 tcttaaaagt tctaaccagt ctaggaaaga cactagcagt cgttccactt gaacagggga
     6421 aaagttgact agaatcagtt atagtctttt ctaataacag gtcatagtca cttataagaa
     6481 ttactaataa catgttttag tattctaatt ggaatatagc tgtaaagtaa gaattacaaa
     6541 ggatttgaga cgtttgcttc aagtcttttt cttttgtccg aaacagatat atagtttcaa
     6601 actatatcaa accctaattt tttacaatta tacagacgct ttcttttctg ttaagatagt
     6661 ttcaacaaca aattttggag gacacatcat gtcaactaca atcatcggtt tccctcgttt
     6721 gggcgaattc cgtgaattaa aatttacaac tgaaaaatac tttagaaaag aaatctcaga
     6781 agaagaactt ttagcagcag caaaagactt gcgtgctaaa cactggaaca ttgtcaaaga
     6841 aaaaggcatc actgaaattc catcaaatga cttttctcac tatgataact tcctagatgc
     6901 ggctttcctt ttcaacgtgg tgcctgcatc cgttcaaaac ttggacttgt ctgaccttga
     6961 gcgctacttc gctttgggac gtggttacca gggagaaaaa ggggacgttc gcgcccttcc
     7021 aatgaagaaa tggttcaaca ctaactacca ctacatcgtt cctaaatttg aaaaagacac
     7081 tcaagtaaaa ttggctggtc acaagatttt cgatgagttc caagaagcaa aagaacttgg
     7141 attgaacact cgtcctgttc ttgtaggtcc attcactttc cttcaattgt cagactttga
     7201 agaaggcgtg aaagcagacg acttcgtaga tagcttagtg gttgcttacc aagaagtttt
     7261 tgctaaattg gctgaacttg gtgcgactcg catccaattg gatgaagcgg ctcttgtaaa
     7321 agacttgaca gctgaagaaa aagctctctt cttgaacctt tacaacaaac tcttggctga
     7381 caaaaaaggt cttgaagtct tgcttcaaac ttatttcggt gatgttcgtg acgtatacgc
     7441 tgaccttgta aacttgccag tagatgctat cggtcttgac tttgttgaag gtaagaaaac
     7501 tcttgaactc gttaaaggtg gcttcccagc tgacaagact ctctatgtag gtattgtcaa
     7561 tggtaaaaac atctggcgca acaactacga aaagagcttg gctgttcttg agcaaatccc
     7621 agctgaaaac atcgttttga caagctcttg ctcacttctt catgtgccat ttacaactgc
     7681 taatgaagaa tttgaaccag cactcttgaa ccactttgcc tttgcagttg aaaaattgga
     7741 tgaaatccgt gacttggatg ctatccgcaa tggtcaaggt tcagaagctc ttgcagccaa
     7801 caaagaactc tttgcgactg agcgtgttgg tgaaaatgct gaacttcgtg cgcgtatcgc
     7861 tggcttgaca gacgcagact acactcgttt gccagccttt gcagaacgtg aagctatcca
     7921 agaagaagct ttcaaacttc cagctcttcc aacaacaacg attggttcat tccctcaaac
     7981 aaaagaagtt cgtgccaaac gtttggctta ccgtaaaggt gaattgtctc aaaaagagta
     8041 cgatgctttc cttgctgaaa cgattgacga atggatcaag tggcaagaag atattgactt
     8101 tgatgtcctt gttcacggtg aatttgagcg taatgacatg gttgagtact tcggtcaaaa
     8161 cttgtcagga tacctcttct ctaaaaatgg atgggtacaa tcatacggta tgcgtggggt
     8221 aaaaccacca atcatctggg gtgatgtcac tcgtcttaac cctatcactg ttaaatggtc
     8281 tagctatgca caaagtcgta caaacaaacc tgttaaaggt atgttgactg gacctgttac
     8341 catccttaac tggtcattcc cacgtgaaga catctctatc aaggattcaa ctcttcaaat
     8401 cgcccttgct atcaaggatg aagtacttga ccttgaagct gctggtgtga aaatcatcca
     8461 aatcgacgag gctgctcttc gtgaaaaatt gccactccgc cgtagcgact ggtacgaaga
     8521 ctaccttgac tgggctattc ctgccttccg cttggtacac tcaacagtag cgccagacac
     8581 acaaatccac actcacatgt gttactcaga atttacagat atcatcccag ctatcgataa
     8641 catggatgca gacgttattt cctttgaagc tagccgttca aaccttgaaa tcttggacga
     8701 actcaaagcg aaaaacttcc aaacagaagt gggacctggg gtttacgata tccactcacc
     8761 tcgtgtacca aatgaaggcg aaatcgacaa cacaatcgaa gccatccttg ctaaagtgcc
     8821 aagcaagaaa gtttggatca accctgactg tggtttgaaa acacgtggta tcccagaaac
     8881 aaaagaaagc ttgatccgcc ttgttgaagc agctaaagct gcgcgtgaga aattgtaaga
     8941 ttggatttct ctccatacag tggttgttca aaaagaaatc aactagaaaa ggttattaca
     9001 tatgtcacgc caaacaccgt cactctcatt tgaagtgttc cctccaaacc cagccgtggg
     9061 taatggtaac attatttcag ccttgcagga tatgcaggag ttggctcccc attttatcag
     9121 tgtaactgcc agcaataata aatttaatat caaggaaacg acggttcgtt tggctgactt
     9181 tatccaaaat gacttggcga ttccgaccat tgcccacttg ccagctattt atctaactaa
     9241 ggacaaggtt gctgaaacta ttgctgactt ggacaaagtt ggggtgcaga aaatcttggc
     9301 tcttcgtggg gatattattc cagacgtaga accacaaaag gatttccgct atgcaaccga
     9361 cttgattgag tttatcaagg aacaagcccc tcactttgat attattggag cttgctatcc
     9421 agaagggcat ccagattcgc caaatcagat ttcagatatt caaaatctta agaagaaagt
     9481 ggatgcaggc tgttcgagcc tcgtaactca gctcttcttt gataatgagc gcttctatga
     9541 ttttcaggac aagtgtattt tggctgggat tgatgttccc attcatgcag gaattatgcc
     9601 aattctgaat cgaaatcagg ctctccgact cttgaagact tgtgagaata tccatcttcc
     9661 acgcaaattt aaagccatct tagacaagta tgagcatgac cctgagtcgc tcagagcagc
     9721 aggacttgcc tatgcagtgg accaaatcgt ggacttggta actcaggatg ttgccggtgt
     9781 gcatctctat acgatgaata atgctgatac agcaaaatac atccatcaag caacccatgc
     9841 cttgtttaat caccagtctc taggataata aaagcaaacc attcttctca ggtgagggga
     9901 atggttcctt tttaatggta aagacctcac tttttaagaa aaatatgata aaataaattc
     9961 tgtacgtact tgatacaaag atgaaggtat taaataatac aatgcattta aactgtaaga
    10021 tttaacgaca cgagtgagtc aaatcgatgt tatttgacga gagagttagt aaagcattta
    10081 gctaatcgcc cgatagcgat tagcgtgaaa gctttagata ctttagtatt aaacctttct
    10141 aatgatgtat cttgaaaact gaacttgcct acaactctgt ggaaaaagat aaatctccct
    10201 aggagcagtc gctccgtcgt tcgatttcct attttccttt gagttgcttg acggcttggt
    10261 atcttgatga cttgaatttt ggtctcattt cttaggaaaa aagataagct tcctagcact
    10321 cgtcgagtgc gacgtcacct tcctattttt ctacagaaat gttcggcaag ccgaaccgtc
    10381 caaaatatct tgtgtaattg aacacggccg aaaagctgtg taaaaaagat aaactgtctt
    10441 gtcttcatcg aagacttcgt cagtttccta tttttacttt gcttttgacg tccttggtat
    10501 cttgatcttt gtaggcaagg cgtataattt catcaatcca aaggggatta aaatggcaaa
    10561 acaagtgttt caaacgactt ttgcgggtcg tgagttaatt gtagaaactg gtcaggttgc
    10621 taagcaagca aatggctctg ttgtcgtacg ttacggtgag tcaactgtct tgactgctgc
    10681 cgttatgtct aagaaaatgg caactgggga tttcttccca ctccaagtca actacgaaga
    10741 aaaaatgtat gcggctggga agtttcctgg tggctttatg aaacgtgaag gacgtccttc
    10801 aacagatgcg accttgacag cgcgtttgat tgaccgtccg attcgtccta tgtttgcgga
    10861 aggtttccgt aatgaagtcc aagtcatcaa tacagtgctt tcttatgatg aaaatgcatc
    10921 tgcaccaatg gctgctatgt ttggttcatc tttggcactg tctatttcag atattccatt
    10981 tgacggacca attgctgggg tacaagtggg atatgtagat ggccaaatca tcatcaaccc
    11041 aagtcaagaa caagcagagc aatctcttct tgaattgaca gtagctggaa ccaagcacgc
    11101 tatcaacatg gtagagtctg gtgccaaaga attgtcagaa gaaatcatgt tggaagcgct
    11161 tcttaaaggg cacgaagctg tcaaagaatt gattgccttc caagaagaaa tcgttgctgc
    11221 tgtcggtaaa gaaaaagcag aagtggaatt gcttcacgtg gatgctgaat tgcaagctga
    11281 aatcattgca gcctacaaca gtgacctcca aaaggcagtt caagtagaag agaaattggc
    11341 ccgtgaagct gcaactcaag cagtgaaaga ccaagtgact gccgtttacg aagaaaaata
    11401 tgcgaaccac gaagaatttg accgtattat gcgtgatgtg gctgaaatct tggaacaaat
    11461 ggaacacgca gaagtgcgac gtttaattac agaagacaag gtgcgtcctg atggtcgtaa
    11521 ggtcgatgaa atccgtcctt tggatgcggt tgttgacttc cttcctcgtg tacatggttc
    11581 aggtctcttt actcgtgggc aaactcaagc tctttcagtc ttgaccttgg ctccgatggg
    11641 agaaactcaa atcattgatg gtttggatcc agagtacaag aaacgcttta tgcaccacta
    11701 taacttccct caatattctg taggggaaac aggtcgttac ggtgcgccag gtcgtcgtga
    11761 aatcggtcac ggtgcccttg gtgagcgtgc tcttgctcaa gtcttgccaa gcttggaaga
    11821 attcccatac gctatccgtc tagtagcaga agttttggaa tcaaacggtt cttcatctca
    11881 agcttctatc tgtgcgggaa ctcttgccct tatgactggt ggtgtgccaa tcaaggcgcc
    11941 agtagctggt attgctatgg gacttatctc agatggaaat aactacacag tattgacaga
    12001 tatccaaggt ttggaagatc actttggaga tatggacttc aaggttgcag gtactcgtga
    12061 tgggattaca gcccttcaaa tggatatcaa gattcaaggg attactgcag aaatcttgac
    12121 ggaggctctt gctcaagcca agaaagcgcg ttttgaaatc cttgatgtca ttgaagcaac
    12181 cattccagaa gttcgtccag aattggctcc aactgctccg aaaattgata cgatcaagat
    12241 tgatgtggac aagattaaga ttgtcatcgg taagggtgga gaaaccatcg acaagattat
    12301 cgctgaaaca ggtgttaaga ttgatataga cgaagaagga aatgtgtcta tctactctag
    12361 tgaccaagat gctattaacc gtgccaaaga aattattgct ggtttggttc gtgaagccaa
    12421 agtggatgaa gtttaccgtg ctaaagtcgt tcgtatcgag aaatttggtg cctttgttaa
    12481 cctctttgat aagacagatg cccttgttca tatctctgag atggcttgga ctcgtaccaa
    12541 tcgtgtagag gatttggtag aaatcgggga tgaagttgat gttaaggtta tcaagattga
    12601 tgaaaaaggc cgtatcgatg cctctatgaa ggctcttcta cctcgtccgc caaaacctga
    12661 gcatgatgaa aaaggtgaaa agtctgagcg ccctcaccgc ccacgtcatc acaaggatca
    12721 caaacctaag aaagaattta cagaaacacc aaaagattca gagtaagaaa aggagaaatg
    12781 tatggggtgg tggcacgaaa ccattgatat cgtaaaagaa aatgatccag cggcccgcac
    12841 cactttggag gttttgctga cttatccagg tgtcaaggcc ttggcggccc accgtctctc
    12901 gcattttctc tggaagtacg acttcaaact cttagctcgt atgcacagtc agttttggcg
    12961 cttttggact cagattgaga ttcatccagg agcccagatt gattcaggtg tctttattga
    13021 ccatggttct ggtctggtga ttggagagac agcgattgtt gaaaaaggcg ttcttctcta
    13081 tcacggagtg actctcgggg gaacagggaa agactgtggc aaacgccatc cgactgtacg
    13141 aaagggagcc ctcatatcag cccatgccca agttatcggg cctgtggaaa ttggtgaaaa
    13201 tgccaaggtc ggtgctgcag cagttgtcgt agcagatgta cctagtgatg tgacggttgt
    13261 cggtattccg gccaagattg tccgtcttca tggtaagaaa gatgagcctg ttattcacga
    13321 agtcgaagaa aaacgggagt attatgtcaa taaactcgag caggctaaag atgccagtca
    13381 cagatcgtct ggtttgtaga ggatacctat atgattcaag caagaaacaa gttaagccaa
    13441 gaggagctat ctgaggcgaa aaaagtaatt aactgttgcc aaaactatga cggtacctat
    13501 cgcgatccct atctctctaa catgcttaat tttgacccaa acatgcccgc ctttttcctt
    13561 tattatgaaa aaggcaaact tgttggttta ttaactgtct atgcagatga ccaagatgta
    13621 gaagtgacga tattggttca tccaggtcat cgccgtcagg ggattgcgcg tgcattgttt
    13681 actagttttg agagagaaac agcttctttc cccattcggt cagtcacttt tcagacagaa
    13741 cgtatttttt tagagaatca tcctgacttt gccagtaact ggggactaat cgaggatgaa
    13801 gagacagaaa cttggttagg taaggataga agaccttatc cgttagcaaa tgtttcccat
    13861 cttgaagttt tgttagcaga tagttcgtat caggatcaaa ttagtcaatt aaaatttcag
    13921 gcattttcag aggaacgtga atcgagagaa gttgtggata gatatgtcgc tgaagctctg
    13981 aaagatccag aaagccgact atatatttta ttaaaagctg gtcaggttat tggaacttgc
    14041 acggtagatt tatcgactaa tacgaattac ttctacggtt tagcaatatc ggaacctgaa
    14101 cgtgggaaag gctatggaag ctacttagca aaatccctcg tcaaccaact aattgagcaa
    14161 aatgacaagg aatttcagat tgcagtggaa gatagcaatg taggtgccaa acgtttgtat
    14221 gaaaaaattg gctttgtcaa acagacacag gtggtttatc tgaatgagaa aggagcaagg
    14281 gattccgaag tgtagagaca ttcggactga aatttacttg agctcttagt ttagtgatga
    14341 aactaacagt tcttggattt tagctttcct gattatgatt tatgattaaa atctatgaca
    14401 ccatgtctcg tgatttgcga gaatttgtcc cgattgagga cggcaagatc aagatgtatg
    14461 tttgtgggcc aaccgtgtac aactatattc acgtgggaaa cgcccgttcg acggtagctt
    14521 ttgatacgat tcgtcgctat tttgagtacc gtgggtataa ggttgcctat atttccaatt
    14581 ttacagatgt ggatgataag attatcaacc gtgccaggga agaaggcatc acacctcagg
    14641 aggttgcgga taagtacatc gctgcctttc gtgaggatgt gacggcctta ggcgtgaaac
    14701 ctgcgactcg ccatccgcgt gtagtggagt ttatggcaga catcatccgt tttgtggaag
    14761 acttgatcga gaaaggcttt gcctatgaga gtcaagggga tgtctatttc cgtgtagaaa
    14821 aatcccacaa ctatgctaaa ttggctaata aaaccttgga agatttggag ctaggtgctt
    14881 caggtcgtac cgatgaagaa acggctcgta aggaaaatcc tgtagacttt gctctatgga
    14941 aatcttccaa accaggtgag atttcttggg atagtccttg gggacctgga cgtccgggtt
    15001 ggcatattga gtgttcagtc atgtcgacag agattttagg tgataccatt gatatccacg
    15061 gtggtggagc tgacctagag tttccacacc acaccaatga aattgcccag tctgaagcta
    15121 aaacaggcaa ggcttttgct aactactgga tgcacaatgg ctttgtcaat atcgacaatg
    15181 tcaaaatgtc taagtccttg ggtaacttca ttacggtaca cgatgccctt aaaactcttg
    15241 atgggcaagt acttcgtttc ttctttgcga cccagcacta ccgtaagcct atcaacttca
    15301 cggaaaaggc agttcgcgat gccgagacca atctcaagta tctgaagaat acttacgaac
    15361 aaccatttac aggaaacgtg gatgcccaag agttacaaaa ctttaaagat aagtttgtag
    15421 cagctatgga tgaagatttt aatgctgcca acggtatcac agttgtcttt gaaatggcca
    15481 aatggatcaa ctcagggaac tatgatgcaa gtgtcaagca agctcttgca gatatgttag
    15541 aaatttttgg aattgtcttt gttgaggaag ttttggatgc agagattgaa gacttgattc
    15601 aaaaacgcca agaggcgcgt gccaatcgtg actttgcgac agcagaccaa atccgtgacc
    15661 aattggttac tcaaggaatt aagctccttg ataccaagga tggagtgagg tggacacgtg
    15721 attgatgtca atctcattaa cgggattgcg ctagcctttg agggggatgc ggtgtattct
    15781 atgtatattc gccgtcacct catcctcaaa ggtatgacca aacccaataa actccatcaa
    15841 gaagcaacta agtacgtgtc agccaaggct caggctcgcc tgattgctct catgttggag
    15901 gagcaggtcc taacggaaaa agaagaagaa atctacaaac gtggccgcaa taccaatagc
    15961 cacacaaagg ctaaaaatgc agatgtcgtg acttatcgta tgtccacggg atttgaagcg
    16021 gttatgggct atctccatat gactgagaat ctggaacgtc ttgagagttt ggtttcatgg
    16081 tgcatccaaa aagtggaggg ctagaacatg agggcaaaag aactacaaga ctggtttcct
    16141 gaggctcgga tttcagacca accagtagag aaagagggct atctcacgct ccctttagct
    16201 tctcagcagt ggattttgct ggaggaagct gggctcagcg agcgtgaaaa gcagttggtt
    16261 gcccttttga cccagcagga gcaggctcgt tcgctaaacc cttggtattc ctatctggtt
    16321 gagggcaagg gacaggcacc gcaagttttt aaaaagattc agttggttta ttgccatctt
    16381 tcttattttc agcaggaaaa tctggcttct tggctagata tgatgcggac tctttttccg
    16441 aattgtcaga cagtgctaca ggtcggagct caggattatg ttttcgtgct tcaacaagac
    16501 aaatacactt ctgtaagaga tattttaagt gatacgattg aagcggttga gtatgacttt
    16561 ggacttcgtc tttctatcat gttgggtcag gtttggtctc agacgggaca tcaagcccta
    16621 tcagacttaa tcaaagctga gcgggatttg ttcaagacat ggtggcgtca gggtcaccaa
    16681 ggtgttcata ctttttctca gctctatctt tggagtatgg gagaaagact cgtggacttg
    16741 aagccaatca aggaatgtct acaccagatg attttggatc aagatcagat tcaggaaatc
    16801 attctctctc tttgggaaaa tagtgctgtt ctcactaaaa cagcccagca actctatctg
    16861 caccgcaatt ctctccaata caagattgat aaatgggaag agttgacagg gcttcagttg
    16921 aaagagttga ccgacctgac cttgtgttat caattgattt taggttcttt gtcaactata
    16981 gttggtttgt aaagaagtta atatttggag aagaggattg ccatcttctc catttttatg
    17041 tgcagagtta tagtggcttg atgctgggaa agtacactgt gactgctaaa acattcctag
    17101 aagctgcttt gatttcccta atctatttat gcaaatttta tgttatttta ctataacagt
    17161 tgttgctaag ccaaataata gtggggaagt tcatttagac gtaagcattg aagataatca
    17221 gggaggtagt gggtataatt tcagttctgt ttcaagtagc tcacaaacag ctaaatatga
    17281 aggaactgtt tataataaca attcatcatt atatataacg attgataaaa cgtctgatgc
    17341 aacagctctt ttgaaattaa agttgaataa tgttgataat caacctgcta ctgaagttcc
    17401 tagttcagga attactgtaa aattaaatgc taaagataat gctggaaact ggacaagtgc
    17461 ttcgaataaa aaagaaataa cagtaaaaat tgtttctgct aaaccgacat atccagacaa
    17521 aatcttagtg aaaaatcctg ataatataaa agatacagaa aaaatgccat tattgaaaaa
    17581 ttgaaagagg caaataaaaa tcatccagca ggagctccaa cctttgctaa aggtgaagga
    17641 gagcatgcaa atgatattgt agcaacttat tcagatggta caacttatta tgtaccgtta
    17701 aatgatgtga caaaatatgc gaggtagtgg ctgtaccact cacttattca cctccccgtg
    17761 atttgtagta gtgataggct ttctcactat tattataaaa caaaataaag atcacaacac
    17821 tttttcattc tgtgttgtgc cttgagtgaa acgaaaggaa tgaattataa atatgaaaag
    17881 tatagtcact agcatagatg agcgcttgcg tactcgccta cgagtgatta tctggaagca
    17941 atggaagaag aaat
//
LOCUS       DAVKHZ010000044        17492 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000044 DAVKHZ010000000
VERSION     DAVKHZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.044"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(107..337)
                     /locus_tag="VWW99_001334"
     CDS             complement(107..337)
                     /locus_tag="VWW99_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539168.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(355..996)
                     /locus_tag="VWW99_001335"
     CDS             complement(355..996)
                     /locus_tag="VWW99_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539169.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(1008..1742)
                     /locus_tag="VWW99_001336"
     CDS             complement(1008..1742)
                     /locus_tag="VWW99_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9539170.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1745..1942)
                     /locus_tag="VWW99_001337"
     CDS             complement(1745..1942)
                     /locus_tag="VWW99_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539171.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1942..2148)
                     /locus_tag="VWW99_001338"
     CDS             complement(1942..2148)
                     /locus_tag="VWW99_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539172.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2158..2472)
                     /locus_tag="VWW99_001339"
     CDS             complement(2158..2472)
                     /locus_tag="VWW99_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539173.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(2768..4057)
                     /locus_tag="VWW99_001340"
     CDS             complement(2768..4057)
                     /locus_tag="VWW99_001340"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9539174.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4137..5384)
                     /locus_tag="VWW99_001341"
     CDS             complement(4137..5384)
                     /locus_tag="VWW99_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9539175.1"
                     /translation="MKLLFRNPAYRILTLSCFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGDLGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(5577..6428)
                     /gene="rgg"
                     /locus_tag="VWW99_001342"
     CDS             complement(5577..6428)
                     /gene="rgg"
                     /locus_tag="VWW99_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9539176.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSAFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            6552..6893
                     /locus_tag="VWW99_001343"
     CDS             6552..6893
                     /locus_tag="VWW99_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9539177.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(7102..8805)
                     /gene="ilvD"
                     /locus_tag="VWW99_001344"
     CDS             complement(7102..8805)
                     /gene="ilvD"
                     /locus_tag="VWW99_001344"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9539178.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(9038..9970)
                     /locus_tag="VWW99_001345"
     CDS             complement(9038..9970)
                     /locus_tag="VWW99_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9539179.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSSSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(9967..10824)
                     /locus_tag="VWW99_001346"
     CDS             complement(9967..10824)
                     /locus_tag="VWW99_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9539180.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(10828..12174)
                     /locus_tag="VWW99_001347"
     CDS             complement(10828..12174)
                     /locus_tag="VWW99_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9539181.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(12187..12471)
                     /locus_tag="VWW99_001348"
     CDS             complement(12187..12471)
                     /locus_tag="VWW99_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9539182.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(12475..14505)
                     /locus_tag="VWW99_001349"
     CDS             complement(12475..14505)
                     /locus_tag="VWW99_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9539183.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            14696..15703
                     /locus_tag="VWW99_001350"
     CDS             14696..15703
                     /locus_tag="VWW99_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9539184.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            15706..15906
                     /locus_tag="VWW99_001351"
     CDS             15706..15906
                     /locus_tag="VWW99_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9539185.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            16058..16240
                     /gene="rpmF"
                     /locus_tag="VWW99_001352"
     CDS             16058..16240
                     /gene="rpmF"
                     /locus_tag="VWW99_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9539186.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            16256..16405
                     /gene="rpmG"
                     /locus_tag="VWW99_001353"
     CDS             16256..16405
                     /gene="rpmG"
                     /locus_tag="VWW99_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9539187.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(16497..17057)
                     /locus_tag="VWW99_001354"
     CDS             complement(16497..17057)
                     /locus_tag="VWW99_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9539188.1"
                     /translation="MNISGERAVEGLRDEKERYRAILKTKESVRTIPMSPKVEQILLK
                     YIERNKFQARFNPTYHDMGYIFTRVYIKGKNQQGSPLYHTEISMFLRGGTSQSVKYNK
                     KSGKSYKDIDDLVDFGRPVHIVPHMFRHSFVSVMADKNVSLNVIREFVGHSEDSREIE
                     KIYLHVMQKGKHKIEQAMIDLSKIIS"
     gene            complement(17133..17450)
                     /locus_tag="VWW99_001355"
     CDS             complement(17133..17450)
                     /locus_tag="VWW99_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539189.1"
                     /translation="MKTFKELVDIEGMVFPNSHGVKRVQRFNPDESPCFLLDDESREL
                     LMRKLPFDKINEPTLKKFAENIIVLNRQKHRVSDKSRMVLMNEANYSYSGESFYTTIV
                     EYY"
BASE COUNT         5658 a         3688 c         2941 g         5205 t
ORIGIN      
        1 acaacctcaa aacagtgttt tgagcaacct gcggttagct tcctagtttg ctctttgatt
       61 ttcattgagt ataatacaaa aatccgatga acttcaccgg actcttttat tttgaatttt
      121 tgcctgcttt acgcttttca gcgatttcgg ctgcctttcg aggcaagaca atttccgtta
      181 tgtaagccgt cccaaaacgc agtacacctg caataggagc aaagacaact gctagatagt
      241 tatagaagaa atcgcctttg aaggcataag ctagcgctcc aatgatgaaa aatagaacga
      301 ctgcctgaat cactgctaat aaaattactc gtttcatgtg acctcctgac tctattatag
      361 catgagaatc atcaaaaagc cgactaaatt attcaaagcg tgaagagaaa tactgtagac
      421 cagacctttt ctgctaatgt aagccaaacc caaactaaaa ccaaggctaa aatagacaaa
      481 aaattgttgc acatcacctg gaaaatgaat caaggcaaat agaagactag ataccagaag
      541 aaaaatcagg gttcgtttac tattgtcctg cttaggaaag agatagcgtg ctaacatccc
      601 tctaaaaaca atctcttccg tcaaaggagc aaaaataacc acagcaaaga atgagaaaag
      661 tggttgagac aaggtcaagt ctgtcgctat ttgttgattt actgaaggat catctggcaa
      721 gaagaattga acgaccagag ataagaacca aaccaaggca ggaagccaaa taaatcggtt
      781 aaagccgctc ttctcaatat aaacaggagc tttctgatac catttgtaaa tgccgtacac
      841 atatactcca gccaagacca catagagtag agtaacagca tagggtgaag cgcctaaagc
      901 aagcgacgca gtcgcgagcc cctgaataaa gccatagata aagaaaaagg atagaagggc
      961 tagaagaaac cagccaaggt ttttaagtag tttcatagat aactccttta tttgaaataa
     1021 cgttttacca taggtaactg catcacattg atataaacat ggatggctcc tacaagcaag
     1081 aaagctagta actgaatctc tcctgtcaag aaagaaatga taataagaaa aatatataag
     1141 gctggtaaga catattggtg taattggaat aaaattcgaa aactctgttc caaattagcc
     1201 tgacgctccc cttcatcata agaatttata tagttcaaga catcctttgg tgtagcgaaa
     1261 aattccaaat caaactgacg aacaatcgca atggttttaa aaagagattt ttgagcgact
     1321 aagaatacca caaagagtaa gaaagaaagg aaaaatgttt gagggtttgt atgcaatata
     1381 atcacctcac ttaatgaaat aaaaatagcc aatggaatcg ctacacctgt aatattaaaa
     1441 gcaatggttc caaactcaag attccgatac atttgcacat aataggtttc attcagatcg
     1501 tcatccattt cctcttgata caaggaatga aattttctgc ttttctttaa gaaattgaaa
     1561 gtcaaaaaca tactaatgaa acctatcagt aaacaaatag ctgatatcca tggcatcaag
     1621 gcttttacat ctaaaataat ttcgtgggat tcgacacgtg ccttaaacat ccctacaaac
     1681 atgcccaaga acccccccaa gacaatagac atcaaaaata acaatctacg tttctttttc
     1741 atattcattc tcctttttca cttgctagat ttttggattt cttttcaatc cattcaatta
     1801 ctgggatgag agcaaagtag acccaaacaa attggtcgct ttgataggga ttaaaccagc
     1861 ttaggtccat cccaatcagt agaaatacgc tgactaataa agctatgacc actacataat
     1921 aaatcacttt atacttgttc atcactcgtc ctcctccaaa cgaaataccg attcgactgt
     1981 ttcgttgaaa atttgagata ttttcagggc aatgataatg gatggggtgt actcatcccg
     2041 ttctagtagg ctaatggtct gtctggaaac ccctgccagt ttggctaggt cggtttgatt
     2101 gagaccatcg cgagctcgaa gctcttttag acgatttttt agttgcacgt tacacaccta
     2161 ctctccgtca aattcaacgg tttggatatc ttcaatacgt tgcaacttga atttttcttt
     2221 tcccgtatta tctacacgtc gtagctttac ccattcctca tcaacatcca caacttccca
     2281 gttatctggc ccaatataca ctcccgttat aattggttcc tttccaatca tttcttgcaa
     2341 taatctcgac atttctgcgt ttcctttctc ttttcgctca agttttttga ttttattctc
     2401 tagtttcttg atttttttag aattattaga ataaaagaaa atcataaata gtataaatcc
     2461 tagtacccac attataactc ctttctgctt cctatttctt aacttgaatt cattgtaaca
     2521 tatctttttc tttttgacaa ttatagttgt caaaaagttt atgatttttg tcattttgca
     2581 aaagaaaaag gtcaggagta ggttcctgac cactttatct atcattaata ctcttctaaa
     2641 atctcttcaa accacgtcag cttcacctta ccgtaggtat ggttactgac ttcgtcagtt
     2701 tcatctacaa cctcaaaaca gtgttttgag caacctgcgg ctagcttcct agtttgctct
     2761 ttgattttta ttgagtataa aatcctagtt tttcaaagat ttctgagaag ttttggctga
     2821 ttgtctcaag tgacacttgc acttcttctc gagtttggtt gttcttgacc gtcacttgtc
     2881 cgctttcgac ttcgctctct cctagggtga tgagggtctt agccacaaag acatcggctg
     2941 acttgaactg agcttttagt ttacggttga ggtaatcacg ctctgctttg aaaccttgtt
     3001 ggcgaagagc ctgtaccaat tccaaggcct tgatatttgc cccttcgccc aagactgcaa
     3061 tatagacatc tagggcgttt tcgataggga gggtcacgcc ttgtttttca aggatgagaa
     3121 gcaggcgctc tacaccaagt ccaaaaccaa atccagcagt ttcagggcct ccaaagtaag
     3181 caaccaaacc atcgtagcga ccacccgcac agactgtcaa gtcattgccc tcaatctctg
     3241 tgataaactc gaaaatggtg tggttgtagt agtccagacc acgcaccata ttggtatcga
     3301 tgatgtaatc tactccaaga ttttccaaca tctgacgcac agcatcaaaa tgagtttggc
     3361 tttcttcatc aagaaagtcc aagatagacg gcgcattctc tactgccacc ttgtcttctt
     3421 tttccttaga gtccaagaca cgaagaggat tttcctccaa gcgacgttgg ctatccttag
     3481 acaaggtctc cttgagcggt gtcaaatagt caatcaaggc ttggcggtag gctgcacggc
     3541 tctcaggatt tccaagagtg ttgaggtgca atttgacacc ttgaataccg atttccttca
     3601 aaaaatgggc tgccatagcg attgtttcca catcggtagc tggattgcta gagccaaaac
     3661 actcaacacc aatctggtgg aattggcgca agcgccctgc ctgtggacgc tcataacgga
     3721 acataggtcc catatagtag aacttgcttg gcttttgcac ttctggggcg aagagtttat
     3781 tttccacata ggaacggaca acgggtgcag ttccttctgg acggagggta atatgacggt
     3841 cacccttgtc ataaaaatcg tacatttcct tggttacgat atccgttgta tctccgacag
     3901 agcgactgat aacctcgtaa tgctcaaaaa taggcgtgcg cacttccgcg tagttgtagc
     3961 gtttgaaaat ttcacgggca aagccctcaa cgtactgcca cttggcagat tcagcaggta
     4021 aaatatcctg cgttcctttt ggtttttgta atttcatagg gaatcctctt taaacttaat
     4081 agtcttattt taccacaaat aaagggatta aaacagtgag aaaaaaaaat taggatttag
     4141 atatcatttt tgagattaag aattgttaaa aaaatagcta gcaaggaaag accaacaaat
     4201 agcatccaag tcaactgtat attccatacg gctactagtg aaaaacaagc tgttcccaca
     4261 ggtatggata aggtaaacaa tagacctaaa aaattactag tacgagctag aacctctgga
     4321 gctagatttt tcatgagcat ggcactaatc tttggttgaa ctttaccaga cacatacaga
     4381 gtaaagaaga gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact
     4441 agactaagcc ctacggtcgc ccacatcatc aatctaggca aggactgctt cccaaaataa
     4501 tcattgcccg taaggctact gatgatgact gctactaaaa cacagaattg attgataaat
     4561 agtgcctctg tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt
     4621 ccacccaaat cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag
     4681 tttttctgcc cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg
     4741 tctttgatag aaagcttctc attttttaag ttttcaccat cagcagatga cattgacagg
     4801 ctcaatttgc tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg
     4861 attcccgcaa cgagagaaaa attgttgacc gatagagcta agagccagac tccgaaagct
     4921 tgaccaccaa tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaaa
     4981 tcatcttcag ctactttttc cttaataaga ggcatacgca ggccacctgc aaaatcactg
     5041 atgatatcac taatgacatt gatcaaacac aggctagaaa aggcaaagag actagcttgc
     5101 tgaacaacta gggctgctag aaaaaataga accgcctgaa acaaaccgct atagaccatc
     5161 catttgacct tgtccctcgt gtaatctgcc cgaatcccta caaaaactgt aaagagggtc
     5221 ggaagaatca tgacaatatt cgccatagca acagcaaaag atgcttgtga caaggtcgat
     5281 gcatagacga taaagaccag gttgaaaatc gaagcaccaa aagcattgaa gaagcatgat
     5341 aaagtcaaaa ttctataagc tggatttcta aacaatagtt tcatagataa tctcccttct
     5401 tgttcgaacc tctatatgaa gtatgactat agtatagcac ttagaaattt gagagggaga
     5461 actcttgaat atatgcaaca aataaaatga gcaaaaagaa acgatagaac ttttgtaatg
     5521 acctgagaga gggcaatagt tctatcgctt caaatttttt cttggtttgc agatattcaa
     5581 cattcggtcg tatttctata gtattcggca gatttattac agccaagcat ctcaaaaata
     5641 cggacagcat cctccatctt tttctggcct tccttgactc taccttgctt gctatcaagg
     5701 agaccttctg cccacagata aacaattcgg aaataggtct cattttcctt gtagaaatgc
     5761 tcttcgataa cacgtttaaa ataataggca ttggtaaatt cttcacactc aatactagct
     5821 aaaaagccat tcaatagtat agtatgaaaa aagtttcgat taccagacat ttccattaga
     5881 aaatcagatt tacgtaccat ttctcgtaca tatctagtaa aaagagaaac agataaaaag
     5941 gcagaactga ctgaaaataa attgagttca tagattcccc agatctcggt agaaaacaaa
     6001 taatcatgaa ggacttttcc ttcctctgct gttaactcta ccctttcatc catgctcttc
     6061 atataagact tgatcataat ggcatttaga atatgtttct gtttgttgtg agaatgggca
     6121 tgcttttgat actccctgcg atataagtcc tcaagaggtg ctatattctt tggttccaag
     6181 acatctgtaa tttcttttct caactcagaa tctgtatcat actggaaacc tctcgccaga
     6241 aagaggatct cctccacact ggcagatata ttttccagag caaatagaaa cttttccacc
     6301 gaaagctcac tctgacctgt ttcaaaacgg gacaacatag acggcgaaaa ttgtcctccg
     6361 gttgcttgtc tcagtgagat atttcttgat tctcgtaatt gtctaaagac ttttccaatc
     6421 tgctccatag acttcccctt gattccgtat tttcttcatt ttatcatatt tttcagaaaa
     6481 ttcatcaaaa acttgccaaa ttgtcagaat tatgagaaaa tagaggatat ttatcacgtg
     6541 gagggactgc tatgagagac gatatcaaaa tcaatgaccg tgctttggcc ttgcaagacc
     6601 aaattatcga aaaactagag aaagtttttg atacagatgt ggaattggat gtttacaatc
     6661 taggtctgat ttatgaaatc aatctggatg aaacggggct ctgcaagatt gtcatgacct
     6721 tcaccgatac tgcctgtgat tgcgccgaaa gcctgcctat tgaaatcgtg gcaggtctga
     6781 aacaaatcga gggtatcaaa gatatcaagg ttgaagttac ctggtcgcct gcctggaaaa
     6841 tcacacgaat cagtcgctat ggccgtattg cccttggact accacctcgt taagcaggcc
     6901 aatcactttt aaagatgaaa aaagcaagcc gaaaatggct tgctttttga tactcaatga
     6961 aaatcaaagg gcaaactaga aaactagccg caggttgctc aaaacactgt tttgaggttg
     7021 tagatagaac tgacgaagtc agtaaccata cctacggcaa ggcgacgttg acgtgatttg
     7081 aagagatttt cgagtatgag tttatttttt acctgacttg tccatattcc agaagtctgt
     7141 cacggctccg cgtgaagcag atgatacgat gtgggcatat ttaccgagga caccacggct
     7201 gtaaagtggt ggcaaggttg tttctgcctt gcgtttttca agttcttctt cggatacggc
     7261 catggaaatt tctttggtat cttggtcaac cgtaacgata tcgccggtac ggagataggc
     7321 aattggtcca ccatcctgag cttcaggagc gatatgtcca acaaccagac cataagtacc
     7381 accagagaaa cgtccgtccg tcaagagggc caccttatct ccctgacctt taccaacaat
     7441 cattgatgaa agtgacagca tctcaggcat accaggacca cctttaggtc caacaaaacg
     7501 aacaacgact acatcgccat caacgatttc atctgtcaga acggcctgaa tcgcatcttc
     7561 ttctgagtca aagaccttag ctggcccaac gtgacgacgc actttaacac ctgatacctt
     7621 ggcaactgca ccgtcaggag caaggttccc gttcaagatg ataagcggac catccgcacg
     7681 ttttggattt tcaagtggca tgataacttt ttggcctgga gtcaagtctg caaagtcagc
     7741 caagttttca gctacagtct taccagtaca tgtgatgcga tctccgtgaa ggaaaccatt
     7801 tgccaacaag tacttcataa ccgcagggac accaccgact tcgtagaggt cttggaagac
     7861 atactgacca gatggtttca agtcggccaa gtgaggcaca cgttcttgaa tcgtattgaa
     7921 gtcctcaagt gacaagtcaa catttgcggc atgggcaatg gcgagcaagt gaagagtggc
     7981 gtttgtagaa ccaccgagag ccatcgttac agtgatagca tcttcaaagg cttcacgagt
     8041 caagatatct gatggtttga gaccaagttc caacatctta acaacagcac gtcctgctgc
     8101 ttcgatatct tctttcttat cagctgattc agctgggtga gaggatgacc ctggcaaact
     8161 catccctaga acttcgatag cagttgccat ggtattagca gtatacatac caccacaacc
     8221 accagggcca gggcaggcat tacattcaag acgtttcacg tcctcagctg tcatgtcacc
     8281 gtggttccat tttccgatac cttcaaagac agaaaccaag tcgatatctt taccatcaag
     8341 atttcccggt gcaatagttc caccataggc gaaaatagct gggatatcca tattagcaat
     8401 agcaatcata gatccaggca tgttcttgtc acagccaccg atagcgacga aggcatccac
     8461 gttgtgacca ctcatagccg cctcgatgga gtccgcgatg atgtcacgag atgttagaga
     8521 gaaacgcata ccaggcgttc ccatagcgat cccgtccgct acggtaatgg ttccaaactg
     8581 tacaggccaa gcgcctgcag atttgacacc ttctttagcc agtttcccga aatcatgcaa
     8641 gtgaatgtta catggtgtat tttccgccca agtcgaaatc actcccacaa tcgatgtttc
     8701 aaagtcctta tctgtcatac cagtcgcacg aagcatagca cggttaggtg atttaaccat
     8761 gctgtcatag atgctactgc ggtgacgttt atctaattca gtcatcttat ccctcccatt
     8821 tcagttttta ctattatagc acaatttaaa ggcaatgaac agaataaaat tcttgaattt
     8881 tcagaaaatt ctatacgcat gtgaaatatt taaaattaaa aacaacaaag cggattagtg
     8941 cactttctga tgaccagaat atgcttttta atccgctttc tttaaataac gtaatgtaat
     9001 ttttacagaa attctttcag ataagtgtat ttaacatcta tcttgcatta taaatttcta
     9061 gaaccttctc ttttatattc gattcactca aaccatactc attaagaaga taatccattt
     9121 tccctacttg accaaatctt tcttgaacac ccatccgatg aatttttgtt attccatcat
     9181 cagagaataa ttcacataaa gcactgccaa ttccacctat ctgattgtgg ttttctacag
     9241 taaatatagt ttttccactt aacattgttt ttatctgttc tggtatcggt ttgattctaa
     9301 ataaatctat cacacctact gaataaccta atttagacag ttcatctgca actcgaatac
     9361 ttggagcaac cattatgcca gaagcaacga ttacaagatc ttcaccatgt cttaactcaa
     9421 tgtagccttt agaaaaatct tctccacctt gatatacagc ctctggagct tttctaattg
     9481 ttcgaatata ttttagtcct tttaagtcta atgtctgatt caatatttca cgaaattgga
     9541 tatcatcagt tgcttcgaaa atgattgatt taggaattaa acgtaacaag ccaatttctt
     9601 caaatggcat atgtgtccca ccattcattt ctgcagttac tcctgcatct gatccaatca
     9661 cagtggcatc caattgtgcg tatccaagag aaataaataa ttgatcaaat acccttcgtg
     9721 aagaaaaagg accaaatgta tgaagataag gtctaaaccc ctgaatagac aagcctgctg
     9781 caaggccgac catttctgct tccataatcc caacattcac ataacggtct ccaaagtcct
     9841 tttcaagatt atgagtagcc atcgaacttg acaaatcggc ttctaagact actatatcag
     9901 aagaactttg attagcctct aaaaggaagt ctctatatac atgccgtaat tctttcgtac
     9961 ttctcatcat tctgtttcct ccaattcctg acttaatctt tctacaactg aagttaacat
    10021 ttgtttctcc tctacagtag ggcgaagatg atgattggat ttcatttctt ccagctcttg
    10081 aaccccttga cctttaatag tatctaatac aatgcactta ggtgatgaat tatttgactg
    10141 ttttaattgg acaatccctt cataaatttc tctaatatct gaacccttga ccctaatgga
    10201 ttcaaatcca aatgctgaaa atttttctac gaaatcacct ggattacaaa tatcctttgt
    10261 aaaaccatct aattgttttt tgttatcatc aacaaataca attaagttgg ataactgttg
    10321 atgagaagca aactgtatag cctcccaaca ttgtccctca tttaactcac catctccaac
    10381 aatagcataa gtataaaagg gactctttct tattctctga ccatatgcaa gtccagttgc
    10441 aacactaatt ccttgtccta aagagcccgt tgtcatatct atgcctggcg ttagatttct
    10501 atcaggatga gacggtaatt tggttccatt tgtatttaaa gaatatagga attctttgtc
    10561 aaagaaacca ttcaaataga gtgtactgta tagagctggt cctccatgac cttttgataa
    10621 tatgaaataa tctctatctc gtgctgcaaa tatttctgga gtcattggca ttatttcacc
    10681 ataaagcact gctaaaactt ctacgataga cagacttcct ccgtaatgtc cgaatccaag
    10741 atgattcaat gttctaagag tatttaatcg gatgttagtc gcaaattttc ttaactcatc
    10801 ttctctattt ttacttaaaa tcatccctta ttcctccttt gcagatggct ttttaataaa
    10861 ggatactcca aacataactg ctagaataag aacaagacca atcacaatgc ctgcttgtga
    10921 gccaaattga tttaacattc ctaaaataat tcctgataga ccaaaatctg catctgagaa
    10981 agttgatcct tggaaaccaa gtcctcccaa aactggcatt aaaaagactg gaagaaaact
    11041 gattaaaata ccttgtaaaa atgctccaat agtggctcca cgaacaccac cagatgcatt
    11101 cccaatgaca cctgcagtcg ctccacagaa gaaatgaggc acaacacctg gtaagataac
    11161 aaccgttcct gaagcaatca taattgccat acttactaaa ccaccaataa aactagagat
    11221 aaatccaatt agaactgcat tgggtgcata agtataaaca atcggacaat ccaaagcagg
    11281 ttttgaatta ggtacaagac gctctgaaat acctttaaag gctggaacaa tttcgcccaa
    11341 aataaggcga acacctgcta aaataacaaa tacccctgct gcaaattgac ctgctaattg
    11401 taaagcataa actagaccac ttgtaccact actgatttct ttttctatat attctgaccc
    11461 tgcaaagata gctacaataa tgtaaataac tgccatagat aaagtgatac taacagtact
    11521 atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc tctgttgatt ttgatttgtc
    11581 accgataagg ctaccagtaa aaccactcaa ccaatatccc aaagaactga aatgacctaa
    11641 agccaccttg tcatttccag ttaattgaac catatatttt tgcacaaatg ctggggaaat
    11701 actcataata ataccgagtg ctaatcctcc tagtaagatg agaggcaagc tagtaaagcc
    11761 agcaactgat aaaatgaccg caatcataca tgccatatat agagtgtggt gccctgttaa
    11821 aaaaatatat ttaaatcgag taaaacgagc gattaagata ttgaacacca tgcctgcaaa
    11881 cataatcatt gcagtagctg agccatatgt tgttaaagct acagctacaa ttgcttcatt
    11941 attcggcaca acgccagata aatgaaaagc atgctcaaac atggtaccaa atggatttaa
    12001 agaattttgt acaattcctg caccaccaga tacaactaag aaaccaacaa aggtcttaat
    12061 tccaccttta ataatatcag gtaatttctt cttctgaaga actaatccta aaattgcaat
    12121 taaagctact aaaatagctg gtgtactaac aatatccaat atgaacttca tcatgacgct
    12181 agcctcctat ataagtcctt tttcttcaca aagtttagta attaattctc gtagttcatc
    12241 catatcaata atactattta agatacgaac atctccaaga tgactagctg aatcagctag
    12301 atcacgacca acaatccaaa tatcagctgc atttggatct gctccaccta aatcataatg
    12361 ttcaacttct acatccgaaa cattcaaatc actcaataca gattcaatat tcatctgtac
    12421 cataaaactt gaacctaatc ctgaaccaca agctgtacca atttttaaca ttatctaatc
    12481 ctcctgttta attatcattt taatgtcatc atagtttttt gatgaaatta aagtttgaac
    12541 atgattttta tctcttaaaa ttgttgttaa atgtgacaaa gcctttaaat gactctcatt
    12601 atcaatggct gcaatacaaa tcaacaatct tacctcttgt tctggattat ccaataaata
    12661 aatcggttct tccaaaacta acattgacat tcctatttca ttcacacctt catctggccg
    12721 agcgtgagga attgctactc ccttccctaa attaataaaa ggtccaaact cttctacttt
    12781 ttgaatcatt gcctcagggt agttctccgt tatcttatct tgatccaaaa gcggtttagc
    12841 tgctaaacga atcgcctcct tccatcctaa tttttgcgaa ctaacctgat aggtttcttt
    12901 ggtaataagt tgttctagca ctggtacaat ttcctttcta tcattttttt gataaagata
    12961 attctttaac gccaatctta attccaattc ttgtgtaata attccatatc ttttgacaat
    13021 attcaggatt tgttcaatct caaaatctcc atactctaaa ttcggaaaat cttttaacac
    13081 tagttctact agttgtattg cttgctcttc agtcatcata accgaaacta gataatttgg
    13141 cttttctgtc tccaccttta tggtagaaaa aaccatatca tagtcactac tagctttcac
    13201 ctgtaaatca tcaatctttg aggttcctat aaactcaatt tgaggaaata atgctaatag
    13261 attctctttt aacatcaatg aagaactaac accattagga caaatgattg ctgctttata
    13321 gcatttttga ggcaaagtat ctgctttctt taaataacct ccgaaatgga taacaaaata
    13381 tgctgtttca ctatcaggta tgggattgtc aatagcgtcc atcaagggca tcaaagaatc
    13441 tttgactaat tcaaataaat caggataatg ttctttaaca tgcaatacat attcatttga
    13501 actaggtagg ccgaacttta atctatagta agccggtata aggtggcggc gaagattttc
    13561 tctcaatcct tccctttgtt taaaatgtaa caaagaaata tcttccattc tacttataat
    13621 agcctctgtt aattgattaa agtaaaccgg agcaacatct acttcacctt caaagcaact
    13681 tgataataaa actgtgatat agcgataatc atcctcagaa aacaccgtat ctataattcc
    13741 caaatcaacc actgtatcca ataaaatagt cgttatatct tgaataacag gagatactaa
    13801 tgtctctgaa agacatactc tttcaacatc cctttgatac ctacacagaa tgaatactaa
    13861 accgaaaagg taaactttta attgattaac aataggtact agctgtagct tctcataata
    13921 atctttaact acctgatcaa tcaaatcata agttaatgaa tacccccaac tggataaaac
    13981 ataatccaaa ccccaaatcc ctatggagga ttccagcaac tcactaacca tttgaaaagc
    14041 taaacggtgc ttattccact ctgaaccgtg taaagtataa ccttttgctc tactgtaccc
    14101 tagctccaaa tcattatcta acataatctt tcttaatgat tgaatatcag ataaggttgt
    14161 attcttactt actttcaaaa agtcttggta atgattattc gatataaaat ctaatcggca
    14221 aaaagtgtaa agatagatta aagctaagcg agtcgacttt ggtaaaacca attcatccga
    14281 cttaataata tctgtcaaag actgcttcgt acgatttgat aaactatagc gaccttgttt
    14341 tttatccagc actatccctt tattagctag ataaggcact aaataatcta ttccttcttt
    14401 gacttccttt ataggtaagc tcaccttaac agataattca tataatgata gctcacaatg
    14461 atccatcaaa gtcatcaaaa taactagtgc tctataatca aacatttctc ttcctttcta
    14521 actacaatta tagtgtaaaa attataagaa taaaagagta tttgagcaca atataatctc
    14581 accttttaaa agaaattatt gggctgaaac tttttagtga ttattttcaa ctttctattg
    14641 acttttaaaa acaaagtgat atcatttaga tatcaaataa caggaggtca ttcttatgac
    14701 tgaaaaacta atcaattcaa aaccaaatgg tgtattcgca ttgattctca ttgaattgac
    14761 gctcgtactt ggtgtcttta tatttataat gggtgttggt tcggaaaaca tttttggaat
    14821 tattatcgga cctttactaa tcgtaattgc aggtctagct catgctggtt taaaagttgt
    14881 caaacctcag gaagctctgg ttctgacact ctttggtaat tatacaggta ccatcaaaga
    14941 acctggtttt tactttgtca atcccttcag cgtagcagtc aaccctgcaa accacactcg
    15001 acttggacaa agtggtgatg ttagcacaaa atctcctttt ttaggagcta aatcatcaaa
    15061 tgacaatgat gtaaatcttg aaattggcaa gaaacacatt tccctcaaag tcatgacctt
    15121 gagcaattct cgtcaaaaaa tcaatgattg cttaggaaac cctgtagaaa tcggtatcgc
    15181 ggtaacttgg agagttgtcg ataccgctaa ggcagtcttc aacgttgata actacaaaga
    15241 atatctttca ttgcaatgtg atagcgccct ccgtaatatt gtccgcatct atccttacga
    15301 tgtgtctcct aatgtggata ctacgggtga cgggcaagca gatgaaggta gtctccgtgg
    15361 ctctagcgaa attgttgcta accgtattcg tgaagaaatc caaagtcgtg ttgaggatgc
    15421 tggcttggaa atccttgaag cacgtatcac ttacctagct tatgctccag aaattgctgc
    15481 cgttatgctt caacgccaac aagcatctgc cattattgat gcacggaaga tgattgtaga
    15541 tggtgctgta ggaatggttg aaatggcact agaacggctc aatgaagggg aattggtaga
    15601 acttgacgaa gaacgaaaag ctgccatggt ttcaaatctc cttgtcgttc tttgtggcaa
    15661 tcatgatgca caaccaattg tcaacacagg aagtctttac taaagatggc tgaagctaaa
    15721 aaaaagcaga tcccccttcg actctcaaca aagttatacg ctgcactcgc atcatgggca
    15781 gaagacgact ttcgttcagt caatgggcaa atcgaatatc tcctcacaga gtgtgtcaag
    15841 caacggaaga aagacggaaa atacgtatca gaaaccattg acgaaccatt tgagattgat
    15901 atttaacatc ttctcctgtc tgacaagtcg gacaggagaa ttttttcatc tttttttgtc
    15961 aagtaacttt actttacaaa aaaaatgtgt tatcctagta tggttgatga aaatcagtag
    16021 attgaatcga atataaatac ctaaaggaga aatcaaaatg gcagtacctg cacgtcgcac
    16081 ttcaaaagcg aagaaaaaca aacgtcgtac acactacaaa gtaacagctc catctgtaaa
    16141 ctttgacgaa actactggag attactcacg ttctcaccgt gtatcactta aaggatacta
    16201 caaaggacgt aaaatcgcta aagctgcatc agctgaataa tagaagggag ataccatgcg
    16261 cgtaaatatt acacttgaac acaaagaatc tggtgaacgc ttgtacctta cttctaaaaa
    16321 caaacgtaac actccagacc gccttcaatt gaagaaatac tcaccaaaac ttcgcaaaca
    16381 cgttgtgttt acagaagtta aataatacaa catacagaaa gcctatgaaa tcaacatttc
    16441 taaggctttt tcttttaagt ttagcaaaat aactgaattt taactgatta taataatcaa
    16501 gagattattt tagacaaatc tatcatagct tgttcaatct tatgttttcc tttttgcata
    16561 acatgaagat agattttttc tatttctcta ctatcttctg aatgtcccac aaactctcga
    16621 ataacgttta agcttacatt cttatcagcc atgactgaaa caaaactatg tcggaacata
    16681 tggggaacta tgtgaacagg cctaccaaaa tcaactaggt catcaatatc cttataggat
    16741 tttccacttt tcttattata cttaacggac tgactagttc cacctcttaa gaacattgaa
    16801 atttctgtat gatacaatgg actgccctgc tggttcttcc cttttatgta taccctagta
    16861 aaaatatatc ccatatcatg ataagtcgga ttaaatctcg cctgaaactt atttctttca
    16921 atatatttaa gtaaaatttg ttctactttt ggtgacatcg gaatagttct aacgctttct
    16981 ttagttttta gaattgctct atatcgttcc ttctcatcac gcaagccttc gacagctcgt
    17041 tctccagaaa tattcattaa ctttcatttt tatatttttc ctttcaattt tcaaaaacaa
    17101 agggaaggat taacctaacc tcccctacca agttaataat attcgacaat agtagtatag
    17161 aatgattcac cactatagct gtagttagcc tcattcatca ataccattct agatttatca
    17221 gatacacgat gcttttgacg atttaataca ataatattct cagcaaattt tttcaaagtt
    17281 ggttcattaa ttttatcaaa tggtaatttt ctcataagta actccctaga ttcatcgtct
    17341 aaaagaaagc atggtgactc gtctggatta aacctctgta cacgtttcac cccatgagaa
    17401 ttaggaaaga ccattccctc aatatcaacc agttctttaa atgttttcat attttctttc
    17461 tccttaaatt aaaatcttaa aattaaatat ag
//
LOCUS       DAVKHZ010000045        17202 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000045 DAVKHZ010000000
VERSION     DAVKHZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.045"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            125..346
                     /locus_tag="VWW99_001356"
     CDS             125..346
                     /locus_tag="VWW99_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539190.1"
                     /translation="MYRRLRDLREDHDLPQKQIATILSFTNSAYAKIERGEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            793..1095
                     /locus_tag="VWW99_001357"
                     /pseudo
     CDS             793..1095
                     /locus_tag="VWW99_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1251..2480
                     /locus_tag="VWW99_001358"
     CDS             1251..2480
                     /locus_tag="VWW99_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9539191.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2500..5196
                     /gene="ppc"
                     /locus_tag="VWW99_001359"
     CDS             2500..5196
                     /gene="ppc"
                     /locus_tag="VWW99_001359"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9539192.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYETIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5279..5496
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5609..6613
                     /locus_tag="VWW99_001360"
     CDS             5609..6613
                     /locus_tag="VWW99_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9539193.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6682..7548
                     /locus_tag="VWW99_001361"
     CDS             6682..7548
                     /locus_tag="VWW99_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539194.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7545..8303
                     /locus_tag="VWW99_001362"
     CDS             7545..8303
                     /locus_tag="VWW99_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539195.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8393..10153
                     /gene="dnaG"
                     /locus_tag="VWW99_001363"
     CDS             8393..10153
                     /gene="dnaG"
                     /locus_tag="VWW99_001363"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9539196.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDDNQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10156..11265
                     /gene="rpoD"
                     /locus_tag="VWW99_001364"
     CDS             10156..11265
                     /gene="rpoD"
                     /locus_tag="VWW99_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9539197.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11279..11608
                     /locus_tag="VWW99_001365"
     CDS             11279..11608
                     /locus_tag="VWW99_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9539198.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11723..12766
                     /gene="cpoA"
                     /locus_tag="VWW99_001366"
     CDS             11723..12766
                     /gene="cpoA"
                     /locus_tag="VWW99_001366"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9539199.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12782..14107
                     /locus_tag="VWW99_001367"
     CDS             12782..14107
                     /locus_tag="VWW99_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9539200.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14138..14314
                     /locus_tag="VWW99_001368"
     CDS             14138..14314
                     /locus_tag="VWW99_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9539201.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14373..14507
                     /locus_tag="VWW99_001369"
     CDS             14373..14507
                     /locus_tag="VWW99_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9539202.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14571..15881
                     /gene="obgE"
                     /locus_tag="VWW99_001370"
     CDS             14571..15881
                     /gene="obgE"
                     /locus_tag="VWW99_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9539203.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15891..16049
                     /locus_tag="VWW99_001371"
     CDS             15891..16049
                     /locus_tag="VWW99_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539204.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(16100..17202)
                     /locus_tag="VWW99_001372"
                     /pseudo
     CDS             complement(16100..17202)
                     /locus_tag="VWW99_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         5269 a         3004 c         3836 g         5093 t
ORIGIN      
        1 aaaaaacttg atacataaaa ataaaagtct atataaagga tggtaaaatt cctgttgtcc
       61 gatttggaca atatcctaaa tagttacaat atatggtcta tactttttct taggagaaag
      121 ctagatgtac agacgtttga gagatttgag ggaggatcat gatctgcccc aaaagcaaat
      181 agctacaata ctttcgttta caaattcagc ttatgccaaa attgaacggg gtgagcatgc
      241 gttgacggct gatgtattgg ttaaactctc agatttctat gacgtcagta cagactattt
      301 attgggatta actgattttc ctgataaaat tcgctttaga aaataatctc ctcaatttca
      361 tagagtttga aaatgagtga gattttttat ttgccctttg acaactgaat agcctaaaat
      421 ggtactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga gcgattttaa
      481 aaccatcaat aaaatagagc gatactttat atgccatgat acaaatgata tacaatgata
      541 cttctgaccg ttcagcctgc caacgtaaaa gagcagcaag tgaaattctt atgatgactt
      601 catcagtcat gccacgttga atgtgtgagt ttgttagata aacgcaatta atcctcaaaa
      661 ggttccccga accttttgag ttctacagac gcatcacgtg gagtgtgtaa gcttgttgct
      721 aaaagcgtaa aaaccttgga acgaaaggaa taatagactt tctgcgaaac aaaaatataa
      781 tacaataaaa ctatgaatga tgaagcaagt aaacaattga gcgatagccg tttcaagatc
      841 cttgtaggtg ttcagcgcac gacttttgaa gagatgttag ctgtgttaaa aacagcttat
      901 caacgtaaac gcgcaaaagg tggacgaaaa agcaaattaa gcctagacga tctccttatg
      961 gtaactattc aatacatgcg agaatagagc acttatgaac aaattgcggc tgattttggc
     1021 attcacgaaa gcaacttaat ccgtcggagt caatgggttg aagcaactct tattcaaaat
     1081 ggttttacga tttcaaattc tgccttaatt ctgtaaaaac agtaaaattc gaaggattgt
     1141 aaggtaagag tttttttctt tctgaaaaaa tggtataata gcaatcaaaa ctagaaaata
     1201 aaacggaatt tggaacagat ttgtctgtat cctagtagag tggtgatact atgaagatta
     1261 gtaagaggca cttattaaat tattccatct tgattcccta cttgctttta tctattttgg
     1321 gcttgattgt ggtctattcg accaccagtg ctattttaat tgaagaaggc aagagcgcct
     1381 tgcagttggt tcgaaaccaa ggaatctttt ggattgttag tttgatactg attgccttaa
     1441 tttataaatt gagactagat tttttgagaa atgagcgact aatcatttta gttatattaa
     1501 tagaaatgct tttattgttc ttggctcgtt ttattggtat ttccgtaaac ggggcatacg
     1561 gttggatttc ggttgcagga ataactattc agccagctga gtacttaaaa atcattatta
     1621 tttggtattt agctcaccga ttctccaaac agcaagaaga aatagctact tatgattttc
     1681 aagttttgac tcaaaatcaa tggcttcccc gtgcttttaa tgattggcga ttcgttctcc
     1741 tagttctgat tggaagtttg ggaattttcc ctgatttagg aaatgcgact attttagtct
     1801 tggtttcctt gattatgtat acagttagtg gaatcgctta tcgctggttt tcaaccattc
     1861 tggcgctcgt atctgccgct tctgtctttg tcttgaccac tatcagccta atcggtgttg
     1921 agaccttttc aaaaattcca gtattcggct atgtagccaa gcgctttagt gcctttttta
     1981 atccttttgc cgatcgtgct gatgcaggtc accagttagc taattcttat tttgccatgg
     2041 tcaatggcgg ttggtttggt ctaggtcttg gaaactcgat tgaaaaacga ggttatttgc
     2101 cagaagctca tacagacttt gtcttttcta tcgtgattga agaatttggc tttgttggtg
     2161 ccagtcttat tttagctctc ttgtttttca tgattttgcg gattatcttg gtcggtatcc
     2221 gagcggagaa tcctttcaat gccatggttg cactcggtgt cggagggatg atgttggttc
     2281 aggtatttgt caatatcgga gggatttcgg gcttgattcc atctacagga gtgactttcc
     2341 ccttcttatc ccagggtgga aatagtcttc tagtcttatc agtggcagta gcctttgtct
     2401 taaatattga tgccagtgaa aaacgcgcta aattgtaccg agaattggaa aatcaaccaa
     2461 tgaaccttct gttgaagtag gataaagaaa ggatagttta tgtctcttca aaaattagaa
     2521 aattatagta ataaaagtgt tgtgcaagaa gaagtcttga ttctaacaga attactggaa
     2581 gatattacta aaaatatgct tgccccagag acctttgaaa aaataataca gttgaaagaa
     2641 ttatcaacgc aggaagatta tcaaggtcta aaccgtctag tgactagctt atcaaatgat
     2701 gaaatggtct atatttcacg ctatttctct atcttgcctc ttttgattaa tatttcagag
     2761 gatgtggatt tagcttatga aatcaatcat caaaataata ttgatcagga ctatttaggt
     2821 aaattatcta caacgattaa attggtagca gaaaaggaaa atgccgttga gatcctagaa
     2881 cacttgaatg ttgtccctgt tttgacagcc catccaacac aagtgcaacg caaaagtatg
     2941 ttggatttaa caaatcatat tcatagtctt ttgcgtaaat accgtgatgt taagttgggg
     3001 ttgatcaata aagataaatg gtacaatgat ttgcgtcgtt acatcgaaat tatcatgcag
     3061 acagacatga ttcgtgagaa aaaattaaaa gtgactaacg aaatcacgaa tgctatggaa
     3121 tattataaca gctccttttt gaaagctgta cctcatttga cgacggagta taagcgctta
     3181 gcgcaagcgc atggtctgaa tttaaaacag gctaaaccaa tcaccatggg tatgtggata
     3241 ggtggtgacc gtgatggaaa tccatttgtt acagcaaaga ccttgaagca gtctgcactc
     3301 actcagtgtg aagtcatcat gaactactat gataaaaaga tttaccaact ttatcgtgaa
     3361 ttttctcttt caactagcat tgtcaacgtc agcaagcaag tcagagaaat ggctcgtcaa
     3421 tccaaggata actcgattta ccgcgaaaaa gagctttacc gtcgtgcctt gtttgatatt
     3481 caatcaaaaa ttcaggcaac taaaacctat ctgattgagg atgaagaagt tgggactcgt
     3541 tatgaaaccg ccaatgattt ctacaaggat ttgattgcca ttcgagattc tctactagaa
     3601 aataagggcg agtccttgat ttcaggtgat tttgtggaat tattgcaggc agtagagata
     3661 tttggttttt acttagcatc aattgatatg cgacaagact ctagcgtcta tgaagcctgt
     3721 gtggcagaac tcttgaaatc agcaggaatt cattctcgtt atagcgagtt gagcgaagaa
     3781 gaaaagtgtg accttctctt gaaagaatta gaagaagatc cccgaattct ttctgcgact
     3841 cacgcagaaa aatcagaatt attagcaaaa gaattagcta tttttaagac ggctcgtgtt
     3901 ttgaaagata agttgggaga tgatgtcatc cgtcagacca tcatttcaca tgcaaccagc
     3961 ctttctgata tgctagaatt agctattctg ttaaaagaag taggactggt ggatacggaa
     4021 agggcgcgtg ttcagattgt tccccttttt gaaacaattg aagacttgga tcattcagag
     4081 gaaacaatga gaaaatatct ttctcttagc cttgccaaaa aatggattga ctcacgaaat
     4141 aactaccaag aaatcatgct tggctactct gacagtaata aagatggcgg ttacttgtca
     4201 tcatgttgga ccctctacaa ggctcaacaa caattgactg ctattggaga tgaatttggc
     4261 gttaaggtta ccttcttcca tggtcgtggt ggtactgtcg gtcgtggtgg tgggccaacc
     4321 tatgaagcca ttacatctca accgctcaag tctatcaagg atcgtatccg cttgacggag
     4381 cagggtgaag taattgggaa taaatacggt aacaaagacg ccgcttacta taaccttgaa
     4441 atgctagtat cggcagctat taaccgtatg attactcaga agaagagcga taccaatacc
     4501 ccaaatcgtt atgaaaccat tatggatcaa gtagtggacc gtagttacga tatctaccgt
     4561 gatttggtct ttggtaatga gcatttctat gattatttct tcgagtcaag tccaatcaag
     4621 gctatttcaa gttttaatat tggttctcgt ccagccgctc gtaagactat tactgaaatc
     4681 ggtggtttgc gtgccatccc ttgggtattc tcatggtcac agagtcgtgt tatgttccct
     4741 ggatggtacg gggttggttc aagcttcaag gaatttatca ataaaaatcc agagaatatt
     4801 gctatcttac gagatatgta ccaaaattgg cctttcttcc aatcgcttct ttcaaatgtt
     4861 gatatggttt tgtcaaaatc aaatatgaat attgcttttg aatatgctaa actttgtgaa
     4921 gacgagcaag ttaaggccat ctatgagact attttaaatg aatggcaagt tactaagaac
     4981 gttatcttgg ctattgaagg acatgacgaa ctcttagctg acaatccata tctaaaagct
     5041 agtctggatt accgtatgcc ttactttaat attctcaact atattcagtt ggagttgatt
     5101 aaacgccaac gtcgtggaga attgtccagt gatcaagaac gattgattca tatcaccatc
     5161 aacggaattg cgacaggatt gcgtaattca ggttgataat tttcaagagt gaatgctaaa
     5221 agtgaatatc aaaaaaattc taatagacta ttgacaagta gtttaaaaat gatataattt
     5281 aaccattcag aaaagtaatc atacaaactt tttagagagt ctgtggtagc tgaaaacaga
     5341 taagtggcaa tgatgaaaat tgggctgaat gctatttaga atttgaaatt ataaaaattc
     5401 ggtaagcaca ccttacagtg catctcgtta ttgcgagact gagcgatagg gaaattccct
     5461 ataattgagg tggtaccgcg catcgacgtc ctcacacaag ttttttgtgt gaggattttt
     5521 ttgatggagg ttagtatgga aagaaaacga tggcgtcgct tgtttagata agtgaaatat
     5581 gttaaaggaa ataaaaagga gaaacagaat gaaaaataaa cgtttaattg gaattattgc
     5641 tgcattagca gtcttagtag caggaagctt gatttattct tcaatgaata aatcagaagc
     5701 tcagaataat aaggatgaga agaaaataac caagattggt gtgcttcaat ttgtgagcca
     5761 tccatccctt gatttgattt ataaagggat ccaagatgga cttgcagaag aaggatataa
     5821 agatgatcaa gttaaaattg attttatgaa ctcagaaggt gaccaaagta aggttgcgac
     5881 aatgagtaaa caattggttg caaatgggaa tgaccttgtg gttggtatcg caacaccagc
     5941 agcccaaggg ttggctagtg caacaaaaga cctaccggtt atcatggccg ctattacaga
     6001 cccaattggt gctaacttgg ttaaagattt gaaaaaacca ggtggcaacg ttacaggggt
     6061 atctgaccac aatccagctc aacaacaagt tgaactcatc aaggctctga caccgaatgt
     6121 gaaaacaatc ggagctcttt actcaagtag cgaagacaat tcaaaaacac aggtcgaaga
     6181 atttaaggct tatgctgaaa aagcaggtct gacagtggaa acatttgcag ttccttcaac
     6241 aaatgaaatt gcctcaactg tcactgttat gactagcaag gtagatgcta tttgggttcc
     6301 aattgataac accattgcat caggatttcc aacggttgtc tctagcaatc aaagttctaa
     6361 gaaaccaatt tatcccagtg cgacagctat ggtagaagta ggtggtttgg catcagttgt
     6421 aattgaccaa catgaccttg gtgtggcaac aggtaaaatg attgtgcaag tcttgaaagg
     6481 tgcaaaacca gccgataccc cagtcaatgt cttttcaact ggtaagtcag tcatcaataa
     6541 aaaaatagca caagaactag gtattactat tcctgagtct gttctcaaag aagcaggaca
     6601 agtcatcgaa taaataaggt agggaggagg agactcttcc cattttttaa aaagatgaat
     6661 atagaaagga ttaaataaaa tatgattgtt tccattattt ctcaaggatt tgtctgggct
     6721 attctaggtc tgggaatctt tatgacattt aggattttaa actttccaga tatgacgaca
     6781 gaaggttcct tccctcttgg gggagctgtt gctgtcactt tgataaccaa aggcgtgaac
     6841 ccatttttag cgacacttgt tgctgtagga gcaggttgtt tggctggaat ggcagcaggc
     6901 cttctttata caaaagggaa gatcccaacc ttgctctcag ggattttggt gatgacttct
     6961 tgtcactcaa tcatgctctt gattatggga cgtgcgaatt taggcctgct tggaaccaag
     7021 caaattcagg atgttttgcc ttttgattcg gatttgaatc aactcttgac aggtctcatc
     7081 tttgtgagta ttgttattgc tctcatgctc tttttcttgg acactaaact cggacaagcc
     7141 tatattgcta caggggataa tcctgatatg gctagaagtt tcgggattca tactggacgc
     7201 atggagctca tgggcttggt cttatcaaat ggtgtgattg cccttgcagg tgccctcatt
     7261 gctcagcaag aaggttatgc cgatgtgtct cgagggatcg gggttatcgt tgtggggctt
     7321 gcaagtttga ttattggaga agttattttc aagagtttga gcttggcaga gcgtttggtt
     7381 actatcgttg taggttctat cgcttatcaa tttttagtgt gggcagttat cgcacttggc
     7441 tttaatacaa gttaccttcg tttatacagt gccttgattt tagcagtctg cctcatgatt
     7501 ccaacattta agcaaacaat cttgaaagga gccaagttaa gcaaatgaca gcaattgtag
     7561 aattaaaaaa tgcaaccaaa atcgttaaaa atggctttga tgaagaaaag attattttaa
     7621 atgatgtttc cttagaaatt tttgaacggg actttatcac gattttgggc ggaaatggtg
     7681 ctggaaaatc aactctcttt aacactatag cagggacctt atcactaact agtggaacta
     7741 tccgtatttt aggtgaagat ctcactaagt tttcacccga gaagcgtgcc aagtacctgt
     7801 ctcgtgtctt ccaagatcca aagatgggga cagctccccg tatgacggtc gctgaaaatc
     7861 ttttaatcgc caagtttcgt ggtgaaaagc gtggattgtt accacgacgc ttgactagct
     7921 ataaggatga atttcaggca accattgaaa aagtaggaaa tggtcttgag aaacacttga
     7981 atacaccgat tgagttctta tcaggtggac aaagacaggc tttgagtctc ttgatggcaa
     8041 ccttgaagcg acctgaatta ctcctgttag atgagcatac tgctgccctg gatccaaaga
     8101 ctagtgttgc tttgatggaa ttgacagatg aatttgttaa gaaagatcag ctaacagccc
     8161 ttatgattac tcatcatatg gaagatgctc tcaaatacgg caatcgcttg attgtcatga
     8221 aagaaggacg gattatccaa gatttaaacc aagaagaaaa agcaaaaatg aaaatctctg
     8281 attattatca actctttgaa taaagtaggg aaaatgagtg aaatggttta ctttttttct
     8341 gaaataaagt atactatata aagtaaacta tgataacatg gaggtattgt gtatggttga
     8401 caaacaagtc attgaagaaa tcaaaaacaa tgccaacatt gtggaagtca taggagatgt
     8461 gatttcttta caaaaggcag gacggaacta tctagggctc tgtccttttc atggtgaaaa
     8521 aacaccttct ttcaacgttg tagaggataa gcagttttat cactgtttcg gttgtggtcg
     8581 ctcaggtgat gtctttaagt tcatcgagga gtaccaaggg gttcccttta tagaggcagt
     8641 ccaaatctta ggtcagcgtg ttggaattga ggttgaaaaa ccgctttata gtgagcagaa
     8701 gccagcctca cctcaccaag ctctttatga tatgcacgaa gatgcagcca aattttacca
     8761 tgcgattctc atgacaacga ctatgggcga agaggccaga aattaccttt atcagcgggg
     8821 tttgacagat gaagtgctta aacatttttg gattggttta gcacctccag aacgaaacta
     8881 tctctatcaa cgtttgtctg atcagtatcg tgaagaggat ttactggatt caggcctgtt
     8941 ttatctctcg gatgacaatc aatttgtaga cacctttcac aatcgcatta tgtttcccct
     9001 gacaaatgac caaggaaagg tcattgcctt ctcaggtcgt atctggcaaa aaacggattc
     9061 acaaacttct aagtataaaa acagccgatc gactgcaatt tttaacaaaa gttacgaatt
     9121 atatcatatg gatagggcaa aaaaatcttc tggaaaagct agtgagattt acttgatgga
     9181 aggattcatg gatgttattg cagcctatcg ggctggaatc gaaagtgctg tggcgtcgat
     9241 gggaacggcc ttgagtcgag agcatgttga gcatctgaaa aggttaacca agaaattggt
     9301 tcttgtttac gatggagata aggctgggca agccgcgaca ttgaaagcat tggatgaaat
     9361 tggtgatatg cctgtgcaaa tcgtcagcat gcctgataac ttggatcctg atgagtatct
     9421 acaaaaaaat ggtccagaag acttggccta tctattaacg aaaactcgta ttagtccgat
     9481 tgagttctac attcatcagt acaaacctga aaacagtgaa aatctgcagg ctcagattga
     9541 gtttattgaa aaaatagctc ccttgattgt tcaagaaaag tccatcgctg ctcaaaacag
     9601 ctatattcat attttagctg acagtctggc gtcctttgat tatacccaga ttgagcagat
     9661 tgttaatgag agtcgtcagg tgcaaaggca gaatcgcatg gaaggaattt ccagaccgac
     9721 gccaatcacc atgccaatca ccaagcagtt atcggctatt atgagggcag aagcccatct
     9781 actctatcgg atgatggaat cccctcttgt tttgaacgat taccgtttgc gagaagactt
     9841 tgcatttgct acacctgaat ttcaggtctt atatgacttg cttggccagt atggaaatct
     9901 tcctccagaa gttttagcag agcagacaga ggaagttgaa agagcttggt accaagtttt
     9961 agctcaggat ttgcctgctg agatatcgcc gcaggaactt agtgaagtag agatgactcg
    10021 aaacaaggct ctcttgaatc aggacaatat gagaatcaaa aagaaggtgc aggaagctag
    10081 ccatgtagga gatacagata cagccctaga agaattggaa cgtttaattt cccaaaagag
    10141 aagaatggag taataatggc aacaaaacaa aaagaagtaa caacatttga cgtacaggta
    10201 gcagaattta tccgtaatca taagcaaaaa gggacagcaa cagatgatga aatcaatgca
    10261 agtctggtta ttccttttac cttggacgct gatgggattg aagatctctt gcaacggatt
    10321 caggatgcag gaatttctat cacagataac gaaggaaatc caagtgcgcg tgttcttagc
    10381 aatgaagaag aaccagaact cagcgatgag gacttgattg ggtcaacttc tgctaaggtc
    10441 aatgaccctg tccgtatgta cttgaaagaa ataggggtcg ttcctctctt gaccaatgaa
    10501 gaggagaaag agttggcact ggctgttgaa gctggtgata tcgaagccaa acaacgtctt
    10561 gcggaagcca atcttcgttt ggttgtttcc attgccaaac gctatgtcgg tcgtggcatg
    10621 cagttccttg acttgattca agaaggaaat atgggcttga tgaaggcggt tgacaagttt
    10681 gactattcta aagggttcaa gttttcaact tatgcaactt ggtggattcg tcaggctatc
    10741 actcgtgcta ttgcggacca agctcgtacc atccgtatcc cagttcacat ggttgaaact
    10801 atcaataaat tggttcgtga acagcggaat ctccttcaag aattggggca agatccgaca
    10861 ccagaacaga ttgctgaacg aatggatatg acacctgata aggttcgtga aatcttgaag
    10921 attgcccaag aaccagtatc tcttgaaact cctatcggtg aagaggacga tagccacctt
    10981 ggagacttta tcgaagatga agtgattgaa aatccagtgg attatacgac tcgtatcgtc
    11041 ttgcgtgagc aattggatga aatcttagat actcttacag accgtgaaga aaatgttctg
    11101 cgtctacgtt ttggactaga tgatggaaaa atgcgcacac ttgaagatgt ggggaaagtc
    11161 tttaacgtaa ctcgtgagcg tatccgtcag attgaagcaa aggctttgag aaaactacgc
    11221 caaccaagtc gtagcaaacc gcttcgtgat tttattgaag actaagagtg aggaaaatat
    11281 ggcttataca gaagagcaaa ttgaaaacat caaaacacgg attttaacag ccttggaaga
    11341 agtcatcgac cctgagttgg gaatcgatat tgtcaatctt ggtttgatct atgagattcg
    11401 ttttgacggt gacacagggc aaacagagat tgacatgact ttgacaacta tgggttgtcc
    11461 cttagcagac cttttgacag accagattta tgatgctatg atagaggttc cagaagtaac
    11521 ggatactgag gttaaattag tctggtatcc agcctggaca gttgaaaaaa tgagtcgcta
    11581 tgcccgcatt gccctaggca ttaagtaaac atataaaagc atgtgagaga ctgttggaaa
    11641 agcgaggaaa tttcccctct tttcctctag tctctccttt cttttgctga ttttattcaa
    11701 agaaaatgat ataatagtag ttatggagaa aaagaaatta cgcatcaata tgttgagttc
    11761 aagtgagaaa gtagcaggac agggagtttc aggtgcttac cgtgaattag ttcgtcttct
    11821 tcaccgtgct gccaaggacc aattgattgt tacagaaaat cttccaatcg aggcagatgt
    11881 gactcacttt catacgattg attttcccta ttatttatca accttccaaa agaaacgctc
    11941 agggagaaag attggctatg tgcatttctt gccagctaca cttgagggaa gtttgaaaat
    12001 tccatttttc ttaaagggaa ttgtgaaacg ctatgtattt tctttttaca accggatgga
    12061 gcacttggtt gtggtcaatc ctatgtttat tgaggatttg gtagcagctg gtattccacg
    12121 tgaaaaagtg acctatattc ctaactttgt caacaaggaa aaatggcatc ctctaccaca
    12181 agaagaggta gtcagactgc gcacagatct tggtcttagt gacaatcagt ttatcgtagt
    12241 aggtgctggg caagttcaga aacgtaaagg gattgatgac tttatccgtc tggctgagga
    12301 attgcctcag attaccttta tctgggctgg tggcttctct tttggtggta tgacagatgg
    12361 ttatgaacac tataagaaaa ttatggaaaa tccccctaaa aatttgattt ttccaggcat
    12421 tgtatcgcca gagcggatgc gcgaattgta tgctctagcg gatcttttct tgttgcctag
    12481 ttacaatgag ctctttccta tgactatttt agaagctgcg agttgtgagg ctcctattat
    12541 gttgcgtgat ttagatctct ataaggtgat tttggaggga aattatcggg cgacagcggg
    12601 tagagaagag atgaaagagg ctattttgga atatcaagca aatcctgctg ccttaaaaga
    12661 tctcaaagaa aaggctaaga atatttccag agagtattct gaagagcatc tgttacaaat
    12721 ctggttggac ttttatgaga aacaagccgc tttagggaga aagtaaaaag tgaggtaatc
    12781 tatgcgaatt ggtttattta cagataccta ttttcctcaa gtttctggtg ttgcgaccag
    12841 tattcgaacc ttgaaaacag aacttgaaaa gcagggacat gctgttttta tctttacgac
    12901 gacagataag gatgtcaatc gctacgaaga ttggcaaatt atccgcattc caagtgttcc
    12961 tttctttgct tttaaggatc gtcgctttgc ctaccgaggt tttagcaagg cacttgaaat
    13021 tgctaaacag tatcagctag atattatcca tactcagaca gaattttctc ttggcctgtt
    13081 ggggatttgg attgcgcgtg aattgaaaat tccagtcatc catacctatc acacccagta
    13141 tgaagactat gtccattata ttgctaaggg gatgttgatc cggccgagta tggtcaagta
    13201 tctggttaga ggtttcctgc atgatgtgga tggggttatt tgtcctagtg agattgtccg
    13261 tgacttgcta tctgattata aggtcaaggt tgaaaaacgg gtcattccta ctgggattga
    13321 attagccaag tttgagcgtc cggaaatcaa gcaggaaaat ttgaaagaac tgcgtagtaa
    13381 actagggatt caagatggtg aaaagacgtt gcttagtctt tcgagaatct cctatgaaaa
    13441 aaatattcaa gcagttttag cagcctttgc tgatgttctg aaagaggaag acaaggttaa
    13501 actggtagta gctggggatg gcccttatct gaatgacctc aaagagcaag cccagaacct
    13561 agagattcaa gactcagtca tctttacagg gatgattgct cctagtgaga cggctcttta
    13621 ctataaagcg gcggatttct tcatttcggc atcgacaagc gaaacgcaag gtttgaccta
    13681 cttggaaagc ttagccagtg gaacacctgt cattgctcac ggaaatcctt atttgaacaa
    13741 cctcatcagt gataaaatgt ttggaacctt gtactatgga gaacatgatt tggctggtgc
    13801 tattttggaa gccctgattg caacaccaga catgaacgag cataccttat cagagaaatt
    13861 gtatgagatt tcagctgaga actttgggaa acgagtgcat gagttttatc tggatgccat
    13921 tatttcaaat aacttccaga aagatttggc taaagatgat acggtcagtc agcgtatctt
    13981 taagacagtt ttgtatcttc cgcaacaggt ggttgctgta cctgtaaaag gatctagacg
    14041 catgttgaag gcttcaaaaa cacagttgat cagtatgaga gactattgga aagaccatga
    14101 agaatagaaa gaggaacagc tatgaaaaaa acaattaatg agaagcggtc gtgataaaaa
    14161 gattgcgggt gtttgtgctg gggtggccca ttatctggat atggatccga ctatcgttca
    14221 agtcatttgg ggtgttctta cttgctgtta cggagctgga attgtagctt acattatttt
    14281 atggattatc gcaccagtag caactgacta ttgaaaacta gctcaattca tgctaagata
    14341 atagaaaata gaatgtaacg aaggagagaa aaatggcatt tggagataat ggaaatcgta
    14401 aaaaaactat gtttgagaaa ataaccttgt ttatcgtgat tatcatgcta gtagcaagtt
    14461 tattgggaat ttttgcaact gcaattggtg ccctcagtaa tctataaaat agattcaaga
    14521 aaatttagtg actgggattt cccagccctt ttttaaagtg agaagaaata atgagtatgt
    14581 ttttagatac agctaagatt aaggtcaagg ctggtaatgg tggcgatggt atggttgcct
    14641 ttcgtcgtga aaaatatgtc cctaatggag gcccttgggg tggtgatggt ggtcgtggag
    14701 gcaatgtggt cttcgttgta gacgaaggac tacgtacctt gatggatttc cgctacaatc
    14761 gtcatttcaa ggctgattct ggtgaaaaag ggatgaccaa agggatgcat ggtcgtggtg
    14821 ctgaggacct tagagttcga gtaccacaag gtacgactgt tcgtgatgcg gagactggca
    14881 aggttttaac agatttgatt gaacatgggc aagaatttat cgttgcccac ggtggtcgtg
    14941 gtggacgtgg aaatattcgt ttcgcgacac caaaaaatcc tgcaccggaa atctctgaaa
    15001 atggagaacc aggtcaggaa cgtgagttac aattggaact aaaaatcttg gcagatgtcg
    15061 gtttagtagg attcccatct gtagggaagt caacactttt aagtgttatt acctcagcta
    15121 agcctaaaat tggtgcctac cactttacca ctattgtacc aaatttaggt atggttcgca
    15181 cccaatcagg tgaatccttt gcagtagccg acttgccagg tttgattgaa ggggctagtc
    15241 aaggtgttgg tttgggaact cagtttctcc gtcacatcga gcgtacacgt gttatccttc
    15301 acatcattga tatgtcagct agcgaaggcc gtgatccata tgaggattac ctagctatca
    15361 ataaagagct ggagtcttac aatcttcgcc tcatggagcg tccacagatt attgtagcta
    15421 ataagatgga catgcctgag agtcaggaaa atcttgaaga atttaagaaa aaattggctg
    15481 aaaattatga tgaatttgaa gagttaccag ctatcttccc aatttctgga ttgaccaagc
    15541 aaggtctggc aacactttta gatgctacag ctgaattgtt agacaagaca ccagaatttt
    15601 tgctctacga cgagtccgat atggaagaag aagcttacta tggatttgac gaagaagaaa
    15661 aagcctttga aattagtcgt gatgacgatg cgacatgggt actttctggt gaaaaactca
    15721 tgaaactctt taatatgacc aactttgatc gtgatgaatc tgtcatgaaa tttgcccgtc
    15781 agcttcgtgg tatgggggtt gatgaagccc ttcgtgcgcg tggagctaaa gatggggatt
    15841 tggtccgcat tggtaaattt gagtttgaat ttgtagacta ggagactggt atgggagata
    15901 aaccgatatc tttccgagat gcggatggta attttgtttc cgccgcagac gtttggaatg
    15961 aaaagaaatt ggaagaacta tttaatcgtc tcaatccaaa tcgtgccttg agattggcac
    16021 gaactaaaaa ggaaaatcca tctcagtaaa gaagctaaaa aatcccggct ctttgtcaac
    16081 tgtagtgggt tgaagtcagc taagctcgag aaaggacaaa ttttgtcctt tcttttttga
    16141 tattcagagc gataaaaatc cgttttttga agttttcaaa gtttcgaaaa ccaaaggcat
    16201 tgcgcttgat aagtttgatg agattattgg tcgcttccag tttggcatta gaatagtgta
    16261 gttgaagggc gttgataatc ttttctttat ctttgaggaa ggttttaaag acagtctgaa
    16321 aaataggatg aacctgctta agattgtcct caataagtcc gaaaaatttc tctggttcct
    16381 tattctggaa gtgaaaaagc aagagctgat agagttgata gtggtgtttc aagtcttccg
    16441 aatagctcaa aagcttgttt aaaatctctt tattggttaa gtgcatacga aaaataggac
    16501 gataaaatcg cttatcactc agtttacagc tatcctgttg aatgagtttc cagtagcgct
    16561 tgatagcctt gtattcatgg gattttcgat gaaactgatt catgatttgg acatgcacac
    16621 gactcatagc acggctaaga tgttgtacaa tgtgaaagcg atcaagaacg attttagcac
    16681 acgggaaaag ttgtctagct aagtcatagt aaggactaaa catatccata gtaatgattt
    16741 tgacgcgaca tcggacggct ctatcatatt taagaaagtg atctcggatg acagcttgtg
    16801 ttctaccttc aagaacagtg atgatatcga gcttttcaaa atcttgcgca ataaagctca
    16861 tctccatctc ccgattgaaa cagtcactcc ccggactgtt tcaacgtccc aggacataat
    16921 ctcaggaaga cgcgaaaaat catgctcaaa gtgagaatca ttgagcttgc gaatgacagt
    16981 tgaagttgaa atggccagct gatgagcaat atcggtcata gaaatcttct caatcaactt
    17041 ttgcgcaatt ttttggttga taatacgagg aatttgatga ttcttcttga cgatagaagt
    17101 ttcagcgacc atcatttttg aacagtgata gcacttgaaa cggcgttttc taaggagaat
    17161 tctagaaggc ataccagttg tttcgaggta agggatctta ga
//
LOCUS       DAVKHZ010000046        16578 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000046 DAVKHZ010000000
VERSION     DAVKHZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.046"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            9..1013
                     /locus_tag="VWW99_001373"
                     /pseudo
     CDS             9..1013
                     /locus_tag="VWW99_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1130..1477)
                     /locus_tag="VWW99_001374"
     CDS             complement(1130..1477)
                     /locus_tag="VWW99_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9539205.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1588..3363)
                     /gene="spxB"
                     /locus_tag="VWW99_001375"
     CDS             complement(1588..3363)
                     /gene="spxB"
                     /locus_tag="VWW99_001375"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9539206.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3570..5813)
                     /locus_tag="VWW99_001376"
     CDS             complement(3570..5813)
                     /locus_tag="VWW99_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9539207.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5823..6194)
                     /locus_tag="VWW99_001377"
     CDS             complement(5823..6194)
                     /locus_tag="VWW99_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9539208.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(6205..6600)
                     /locus_tag="VWW99_001378"
     CDS             complement(6205..6600)
                     /locus_tag="VWW99_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9539209.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6784..6882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6966..7772
                     /gene="thiD"
                     /locus_tag="VWW99_001379"
     CDS             6966..7772
                     /gene="thiD"
                     /locus_tag="VWW99_001379"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9539210.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIHPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCT
                     FAAVITAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7982..8614)
                     /locus_tag="VWW99_001380"
     CDS             complement(7982..8614)
                     /locus_tag="VWW99_001380"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9539211.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMDTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8607..9410)
                     /locus_tag="VWW99_001381"
     CDS             complement(8607..9410)
                     /locus_tag="VWW99_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9539212.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9410..9934)
                     /gene="thiW"
                     /locus_tag="VWW99_001382"
     CDS             complement(9410..9934)
                     /gene="thiW"
                     /locus_tag="VWW99_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9539213.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            complement(9939..10631)
                     /gene="tenA"
                     /locus_tag="VWW99_001383"
     CDS             complement(9939..10631)
                     /gene="tenA"
                     /locus_tag="VWW99_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9539214.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKVFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10642..11292)
                     /locus_tag="VWW99_001384"
     CDS             complement(10642..11292)
                     /locus_tag="VWW99_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9539215.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(11294..12679)
                     /locus_tag="VWW99_001385"
     CDS             complement(11294..12679)
                     /locus_tag="VWW99_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539216.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12680..13240)
                     /locus_tag="VWW99_001386"
     CDS             complement(12680..13240)
                     /locus_tag="VWW99_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9539217.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(13502..13590)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13799..14428)
                     /locus_tag="VWW99_001387"
     CDS             complement(13799..14428)
                     /locus_tag="VWW99_001387"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9539218.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALRGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIMKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(14430..15212)
                     /gene="thiM"
                     /locus_tag="VWW99_001388"
     CDS             complement(14430..15212)
                     /gene="thiM"
                     /locus_tag="VWW99_001388"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9539219.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            15296..15388
                     /locus_tag="VWW99_001389"
                     /pseudo
     CDS             15296..15388
                     /locus_tag="VWW99_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(15538..16206)
                     /locus_tag="VWW99_001390"
     CDS             complement(15538..16206)
                     /locus_tag="VWW99_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9539220.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(16421..16509)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         5045 a         3625 c         3029 g         4879 t
ORIGIN      
        1 agtagtttga aataagatgt gaacaactct atcaggaaag tcaaactaat ttatagaaat
       61 attttagcag ctaaggtgta ctgttataga ttcaatacac tatagactgt aatcaaacaa
      121 cgatttggcg aaatgtaaaa aaatatgagg agttcgaact cgactctctc cttcaagaaa
      181 cacgtggtgg ccgtaaccat gcatatatga cgattgagga aaagaaagtc tttcttgccc
      241 gccatttgaa ggctacagag gcaggagaat ttgttacaat tgatgcctta tttcaggctt
      301 ataaaaagga gttaggtcgt tcctacacac gtgatgcctt ctatcaactg ttgaagcgcc
      361 atggttggcg aaatattatg ccacgtccag aacatcctag gaaagcagac gctcaaacca
      421 ttgtcgcgtc taaaaataaa atctcaattc aagaagaaaa gaaagcgctt taaaagcagt
      481 agacgttttc gtaaggttcg cttgatgtac caagatgagg ctggtttcgg tagaatcagt
      541 aaactgggat cttgttgggc tccaatagga gtaggtccac atgtccatag tcactatata
      601 cgagaatttc actattgtta tggagctgtt gatgcccata caggcgaatc atttttctta
      661 atagctggtg gatgtaatac tgagtggatg aacgcctttt tagaagagct ttcacaagct
      721 tatccagatg attatctttt actcgttatg gacaatgcta tatggcataa atcaagtacc
      781 ttaaagattc cgactaatat tggttttacc tttattcctc catacacacc agagatgaac
      841 ccattgaaca agtgtggaaa gagattcgta aacgtggatt taagaataag gcctttcgaa
      901 ctttggaaga tgtcatgaat caacttcaag atgtcataca aggactggag aaggaggtga
      961 taaagtccat cgttaatcgg agatggacta gaatgctttt tgaaagcata tgagtattat
     1021 atgcaatttc tttatataaa aagaccggat tgctccgatc tttcaatagt tcatattctc
     1081 aatttctatt ttaaaaatag ctaaggttaa cgtcaaatga ctacgcgacc tatttcatac
     1141 gataaaaatc aagcactaga ccagcaggtc cttgaactaa taaggactct gttccccaat
     1201 cggttacagt tggtccgtgt aaaaccttta taccaagctc gttcaaccgt ttgtagttct
     1261 ggtctacatc ctcaacctcg atatgaataa tgattcctga ctgaaagttt tccaaaggaa
     1321 ccaaatgatt ttgtgacaac ataaggcagt gactaccaat cgtaaactga gcaaaaccat
     1381 catcagcata atctgccttt ttatccaaaa tatgctccaa gtcagcacag acttggggaa
     1441 catttgaaac gataatatct aattgattta aattcattta ctctcctcca taaaaagacc
     1501 ggattgctcc gatcttttaa agttctgctc tatgaaaatc aaagaataaa gtctacaagt
     1561 ttcatatttg attttcggcg agaggaatta tttaattgcg cgtgattgca atccttcttc
     1621 ttccaagaag agacggaatg gtacgagttc ttctgcttcg tatttttcct tgaaggcttt
     1681 gatagcttct tctgagtgaa gttttggatc caattcaagt acttctactg gaagtggacg
     1741 gtgttgagtg atgcgagcat cgatgacaac agttttacct tctttgttca atttaacagc
     1801 ttctgcaaca actgcatcga tgtcttcgat acggtcaact gtgaatccaa cagctccttg
     1861 agcttccgca attttagcgt agtcagcgtt tgtgaagtct acaccaaaca agtgtttgtt
     1921 tgtatcttcg tatttgttct tgatgaagcc gtactcagca tttgagaaga caaggttgat
     1981 aactggaagg tcgtattgaa cgtttgtgat aacgtctggg tagcacatgt tgaatgctcc
     2041 gtcacccatg atgttccata cttggcgatc tggattgtct ttcttagcag cgataccacc
     2101 aggaagggca atacccattg tcgcaaagag tggagatgta cgccacatgt tcttaggtgt
     2161 catgtgaagg tgacgagtag atgtttgagt agtgttacct acgtcgattg agtagatagc
     2221 gtcttgatca gcatgtttgt tgattgcatt gtaaacttga tacaattgca attcaccctc
     2281 agttttacct tcgagtttgt tcatgtaatc acgccagttt tggttgttct taacgtttgc
     2341 acgccaccat ggagttgatt caactgggtt tactttgtca aggatagctt tagctgcttg
     2401 accagcatca ccaaggattg aagcgtcaag ggcatgacgt ttaccaagtt tgtaagggtc
     2461 gatatcaact tggatgaatt tttcagtgtt cttgaatgct tcgtaaactt cagcaaatgg
     2521 gaagtttgaa ccaaggaaaa gaactgtgtc tgcttcaaag accacttcgt tggctggttt
     2581 ccaaccaaca cgataagcag aacctgtcaa accttcatag ttccattcga aagcttcaaa
     2641 gtttttacca gttgtgatga ttggtgcttt gattttacgt gacaattcag taatcacttc
     2701 accagcttta acaccaccaa atccagcata gataactggg cgttcagcat tgttcaagat
     2761 ttcaacagct ttgtcgattt caacttcgtt caaagcagga gcgatgaatg agcgttcgta
     2821 tgaacctgaa ccgtagtatg agttttcgtc gatttcttgg aaaccgaagt ttactggaat
     2881 ttcaacaaca gctggacctt ttttagaaat tgcagcacgg caggcttcgt caattacttt
     2941 tggcaattgc tcagcgtaag ctacacgttt gttgtaaaca gcgataccgt tgtacattgg
     3001 gttttggtta agctcttgga aagcatccat gttcaattcg ttaactggac gtgatccaag
     3061 gatcgctagg aatggagtgt tatccatagc tgcatcgtaa acaccgttaa tcaagtgagt
     3121 cgcacctgga ccacctgaac caactgcaac cccgattgag ccgccgaatt tagcttgcat
     3181 aaccgctgca agagcacctg tctcttcgtg gcgaacttgt aagaagcgga tatctttgtc
     3241 ttcagccaaa gcgtccatca atgagctgag tgttcctgat gggataccgt agattgtatc
     3301 tacgccccat gttttcaata cgttaagcat tgctgcagat gcagtaattt tcccttgagt
     3361 cataatgata actctccttc aattttttta gatttggaga atacgattac atagaattgg
     3421 aaacgttctc caaattttta ctattccact gtatcatatt tatgctgact tttctaaaaa
     3481 tctgctcaaa actctctatt ctctattcta atacagtttt taaagttctg tcatttctgt
     3541 tttataacaa agaaatctag tcattacttt tagtctattt tactaaaatt taacagaagg
     3601 gaactggtca gaacagatac agaactaaag gccatggcta gacctgccaa ttctgggttg
     3661 agagccagtc caacacctga aaagactcct gctgcaatcg gaattccgac aacattgtag
     3721 ataaaagccc agaaaagatt gagtagaatt cgatgaaagg ttttcttact catatcaaag
     3781 gcacgaacca ctcctaaaag attattggtt gtcaacacca aatctgctga ctcgatggcg
     3841 atatctgttc cagctcccat agcaatcccc acatctgcta cactaagggc aggagcgtca
     3901 ttgataccgt ccccaacaaa ggctactttc cctgactgtt gcagtttatg gatttcatgg
     3961 gctttttctt ctggcaagac gcctgcaatg acctcttcaa ttccgatttg atctgcaata
     4021 gcacgcgcca caccagcatt gtctcctgtc agcatgactg ttcggagacc acgttttttt
     4081 agctgactga tggctagctt agcattttcc ttaggaatat cttgcaaagc aagcaagcct
     4141 ttgatttcat tgtcaacagc taaaaacaca actgtcttag cttctttttc tagttcttct
     4201 agtttatctt gataagtatt agaaatatcc atgccatcca gcattttagc atttccaagt
     4261 aaaacttgtt ttccattgat tcgccctgaa acacctttcc cgtgcaaggc ctgaaaattt
     4321 tcaacagttt gaaactcaag tccagcttca ctcgctcgct taacgatagc ctcagccagt
     4381 gggtgttgag aagcatcttc caaggaggct gccaatccaa acacttctac ttcgtcaccg
     4441 atgatatctg ttaccacagg tttcccttcc gtcaaagtcc cggtcttatc aaagacaaga
     4501 gtttgaactt tctggatttc ctgtaagaca gttccatttt tgaggagaac ccccatcttg
     4561 gcactacgtc ctgtccccac cataagggct gtcggtgttg caagtcccaa ggcacaagga
     4621 caggcgataa tcaaaaccgc cactccgtag agaagagagg acacaaagct agctccaagc
     4681 acgaccacac tatccctgag caagacgaac caaacccaaa aggtcatgat tcctaaaatg
     4741 acaactactg ggacaaaaat ccctgaaatc ttatccgtca agtcctgaat cggcgcacga
     4801 cttgtctgag ctttcttcac aaaatccaca atctgagcca aaacagtctc tgagccaact
     4861 ttttctgctc taaagacaag cgttccacta tgattgatgg ttgagccaat gacagtatct
     4921 ccaactgtct tgtccacagg cagactctca cctgtcacca tggattcgtc aatactagag
     4981 acaccttcta ctacgacacc atcaacagca atcttttcac cgggacgcac tcgaatcagg
     5041 tcgcctacct tgacttgttc caaaggaact tggacataac tatcatcact caagacttct
     5101 gcggttttag cttgcaagtc cagtaatttc tccacagctt gggacgtatt ttttctcatt
     5161 ttttcctcaa aaactgctcc caaaagaacg aaaaagagga taaatccagc actttcgaag
     5221 taaacaggga gaccagcaaa gagagcaact aggctataga aataagccac tagagttccc
     5281 agcgcaacca aggtatccat gttggcattg tgctttttaa aactggccca agcactctgg
     5341 atatatggct tacctgcaac taacataata ggcgttgttg ctaaaaaggt tccccaatgc
     5401 atgacttgat gactaatgct acctgccaac atcccaatca tgagaatcac aagaggcaca
     5461 gtaaagatac tagtaatcca aaaacgttgc aggagagata gagattttcg agtcttctca
     5521 acgactgtat agcttccctt ttgcatcttc atgccacaag aaaattcatg tcgccctaat
     5581 tcttgaggcg taaaacgaat gactttctcc tcatctacgc cgattggttc caagatacct
     5641 tcttcttcaa acagaatttc cttataacag tttgaaggag tagcacgatg aaaggtaatc
     5701 tcagctggaa ttcccttttg aagctggata tgggctggat gatagccttt ttcagctcgg
     5761 atacggattt tttgaatgcc attttctaag cttgctttca caatttctgt catagtctcc
     5821 acctactcta caatcatctt gccgtgcatc atgttcatac cacaagcaaa gccaaactct
     5881 ccagcctgtt caggcgtgat ttccactaca tactcttccc ccattggcag gttcgcatgt
     5941 acaccaaaat ctggaaaaac aatttgatcc agacatggtg aaggatcctt gcggtcaaag
     6001 acaatgcgtg ctggcactga tttctttagg acaatcaact caggagtata gcctcccatg
     6061 acttccactc gaatctcttg gtatccgttt ttttgctggg ctttttgtcc agatttttca
     6121 ggctttttga aaaaccaaaa caagataaat gcgataaggg caatacaaat aatggttaca
     6181 atactattta acatgacgtc tcctttacat acaattacat cttacttctg ttacagcact
     6241 tgatttcttc tctgaaatca cagcttccaa gtcttccaag tcagtctgag taaattcaca
     6301 ttctacaatc aagtcagcca acaaattcct aatcctacgg gaacaaacct tgtctttgat
     6361 atcttggaca agtaaatccc gactttggtc tagagttaaa agggctgaat aaacaaagga
     6421 cttgccttct tttttccgag tcaaacactc tttctcaacc agacgagcca aaagtgtctg
     6481 aaccgtggac ttggaccagt caaaccgctc tgccaaaacc ctaatcaaat ctgtactggt
     6541 ctgctccccc tgcatccaaa taatcttcat gacctgccat tctgcatctg aaatctgcat
     6601 taccatacct ccaaaatcta catttgtcaa ttacactcat cagtatactc ttaaaatcta
     6661 catttgtcaa ttatagaaat aatattttct tcgaaaaata gaattttaat catttgaaaa
     6721 acgatttgca gtcaaatatt actatataaa caataaaaat atgctatact aaagaaaaaa
     6781 gaaaacaacc actaggggtg cgtaaagctg agattaacga ctgttagatc cctctgactc
     6841 aatctagata atgctagctg atggaagtgg aaatgataat ggggactagc agtcttctat
     6901 tgcctttcta aaacagacta gcttgttctt aagaatacaa acttcagttg gttgggaggt
     6961 tttagatgac ttatttaccc gttgctttga ccattgcagg gactgaccct agtggtggtg
     7021 ctggcattat ggcagattta aagtcattcc aagcgagaga tgtctatgga atggctgttg
     7081 taaccagtct tgtcgctcaa aataccagag gtgttcagct aatcgagcac gtttctcctc
     7141 aaatgttgaa agcccaattg gagagtgtct tttctgatat tccacctcag gctgtaaaaa
     7201 ctggaatgtt ggctactact gaaatcatgg aaatcatcca cccctatctt aaaaaactgg
     7261 attgtcccta tgtccttgat cctgttatgg ttgctacaag tggagatgcc ttgattgact
     7321 caagtgctag agactatctc aaaacaaact tactacctct agcaactatt attacgccaa
     7381 atcttcctga agcagaagag attgttggtt tttcaatcca tgaccccgaa gacatgcagc
     7441 gtgctggtcg cctgatttta aaagaatttg gtcctcagtc tgtggttatc aaaggcggac
     7501 atctcaaagg cggacatctc aaaggtggtg ctaaagattt cctctttacc aagaatgaac
     7561 aatttgtctg ggaaagccca cgaattcaaa cctgtcacac ccatggtact ggatgtacct
     7621 ttgctgcagt gattactgct gaactagcca agggcaagag tctttaccag gcagttgata
     7681 aggccaaggc ctttatcaca aaagctattc aagatgcccc tcaactcggt catggttctg
     7741 gtccagtcaa ccatacaact tttaaagatt aagaaaaaaa actctctagt tcccacttta
     7801 agggaattag agagttttta tactcttcga aaatctcttc aaactacgtc agcttccatc
     7861 tgcaacttca aaacactgtt ttgagctgac ttcgtcagtc ttatctaaaa cctcaaggca
     7921 gtactttgag caacctgcga ctagctttct agtttactct ttgattttca ttgagtatta
     7981 attaggaaag aatgtcatgc aactttgtta aaaaggcttg cgtttttgcc tcaatatctt
     8041 ctgcttgcat caaatcacgt acaacagcta caccagctat gccagtgtcc ataagctgat
     8101 caatattctc tgatgtcaag ccgccaatag caactactgg aatggcaacc gtttggcaaa
     8161 ttgttttcaa ggtcgatatc agagtaatgg gcgcattttc cttggtggtg gttgggaaaa
     8221 tggctcctgt acccaagtaa tctgcacctg atttctccgc ttccagagct cttttaaccg
     8281 ttttagcggt gacaccgagg attttttcag gacccaagac tttgcgagct accgaaactg
     8341 gtaattcatc atctccgata tgcagacctg ctgcatcaac cgcaagacaa acatccaacc
     8401 gatcatcgat tatcaagggt acctgataag catctgttat ttccttgact tgttttgcca
     8461 gttgataata ttgattggtt gtgagatttt tttctcgcaa ttggactatg gtaacccctg
     8521 aacggcaggc cgtctcaact tttgcaagaa agctttccac ggaatcttga tagcgattgg
     8581 ttaccagata tagtctaagt gcttctctat tcataaacct ctcctttgat ggtatctagc
     8641 caattttcat ctcttcttag gagcgaaagc tgattgagta cttggtaacg aaattcttcc
     8701 aatcccattc cttgaacaac tattttctca gcagcgatat tgagataaga gactgctaag
     8761 caagaagctt caaaaccagt ctttccttgg ctgagaaaaa cggctgttaa ggctccaacc
     8821 aaatctcctg tccctgttat ccagtctaat tctgcacagc cattctcaag tacagcaact
     8881 tgattctccg aaacaataag gtccttggga cctgtgacta agaatgacat accaggatag
     8941 gtctgacacc agtctttcaa gacttgaagc aaatcctccg tttcttgatc tttagcactc
     9001 gcatcgaccc caacgccgtg atgctttaat ccaacaagac ttcgaatttc tgacatgttt
     9061 cctttaagga ccgtaggtct atagtctaaa aggtctttaa ctaagctctt acgaatggat
     9121 gaagtcgtta cgccaaccgc atctactacc atcgggagag aagattggtt tgcatacaaa
     9181 gctgccatgc ggattgcttt ttccttctca gctgacaaat gccccaaatt gatgaagaga
     9241 gcctggcttt gcttagtaaa atcaagaact tcacggggat catctgccat gacaggtttg
     9301 catcccagag ccaaaatccc atttgccagc atctcacaag aaatctcatt ggtcatacag
     9361 tgaatgaggg aactagagcc tataggaaaa ggatttgtca atgcctgcat cattctatcc
     9421 tttcagcaaa gaaatatcct tgcacttttt taaagaattc ctgcttgatt aaaaatctaa
     9481 atgcaataaa ggaaatcgct gtaccaatca aggttgctcc gaaaaatcga ggagtgtaga
     9541 taaaccaact aagcttagca gccgatcctg taaagagcac cataacagga taggaaacaa
     9601 tagaaccaat aatacctgtt cccacaattt ctcccaaggc agaaaagtaa aattttcgac
     9661 cgtacttata aaagagacct gctagaaggg ctccaaaagt cgctcctgtg agagctaaag
     9721 gcggaatccc ttgagtcgtc atacggataa aggctgtcac tgtagccata gccaaggcat
     9781 aaacaggtcc catcatgatt cccgctagaa tattgactac actggacatc ggtgccattc
     9841 cctcaatccg aaagataggt gtaaggacta catcaagggc aatcatcata gataaaatgg
     9901 tcaatttgtg aacttgtagt tggtgctttc tcatgtttct attcttctcc tttttctaaa
     9961 gactgtaaat cgctcttcca tgtctggtgt tggtaagcca tctcccaaaa cttggcttcc
    10021 atatgaacac tgatgtggaa ggcatctagc attttttgct tatctgtctc atcactttct
    10081 cgatagagct gattgaccaa tgctccctcc tctctgatct gttgctctaa ctcatccgta
    10141 atataagttt caatccattg ttgatagaga ggatttggtg atggtttaag attaagtgat
    10201 ttgcctatat catggtataa ccaaggacaa ggaagcaagc ttgcaaaagc tatggctaag
    10261 ttcggttctg caaattgcct ataaatatga gaaatgtaat gataacaggt tggagcgatt
    10321 ggatgttgct ccatttcctg gtcgttgatt tccaattcct tgaaaaattg ttggcgaata
    10381 aataactcac cctccactaa accctgagca ttttgtttca agagtctttt catctcttgg
    10441 tttgaagtct tatcagccaa aagatgatag atttctgaga aaaccttcag atagtaggca
    10501 tcctgaatca ggtaatagcg gaaaatggca ggttctaaat tcccctcttg taattgtaaa
    10561 ataaagggat gatgaaagga agcctgccaa gctttcttgg ataattccat cgcaatatct
    10621 gtaaattcca taataactcc tttataaaaa tagactggtt tgaagcaata aaaagaaaag
    10681 caggtagatt aattttgttt ttttaggaat ataaaaagtc cgatagctat tcttcaactg
    10741 tgcatgttcg tcatatccgt gagcagatag agctctcagg taaagatggc gccacctaaa
    10801 gactgtcatc agaaccttac tgtaaatcaa gggcgaccaa aaatgtagtt cttgaccacg
    10861 taataggcaa gcttctttga gggacttgat ttcttgctga atgagaggaa aagaattgaa
    10921 taccacaatc aaggcatagg accaagagcg tgatagcccc ttttgagcca agtacaagag
    10981 aagctctttt agtgaaatag aggaaacaaa gacaaggccg atacaaactg tcacaaaggc
    11041 cctcgttcca agcatgactg cctgtgaagc atctccgtgt aactgaactg cccagtagtt
    11101 ggcaaaagat ggtaaaatgg caagtatgat catccaagct aacattttaa atcgacggta
    11161 atagagcata aagagaatac aaaatgcgac taccgaaaga ttcagagcaa tcgaaggaat
    11221 gaaagatgtt tccaaggata aaatcagcaa gaagagactg ataatcggtg tctgggttgc
    11281 tactttgacc atactatctc acctcccctt gggtattgct actctgagat gtaagtggtt
    11341 tggtaatggt cacttctttc acatgccgaa gaccctgact agtcatctca atccaataat
    11401 caaccacaga aatcaaaggg tctaaacgat gactaatgag cagaaaactt cttccttgat
    11461 tcctctcctc cacaatccac ttgcaaaaat aatggcaggc tctatcatcc aaacctgcaa
    11521 aaggttcatc tagcaagatc acggaagcct tactggtcaa gatggtcagg agctgaagaa
    11581 ttttttgctg accaccactt aattgatagg gactcttatc gactgcctgc tccaaatcaa
    11641 aatatcgtaa agcttgaaaa atccgctgat ttctttcaga atcaggtcca tctaattgaa
    11701 gctcctctcg cagactgact cggataaact gcttctcagc ttcctgaaca acaccagtca
    11761 gatcacgata caaactcttt ttctttttca ggaccgaacc cttccatgca atgcgcccct
    11821 tatacttttg aaattgaaga atagaccgaa agagggttga tttcccgaca ccattgtcac
    11881 ccaggataca ggaaatccct tgatagaatg tgaaatcagc aattgaaaag agggggcgat
    11941 ttccaaactc acaagtcgtt ctatccatat ggaatagttc cgggctagaa gcaacttcct
    12001 ttgaagcaac ctgtgtcatc tcataggaag ggatttggaa cacttccctt agttttccgt
    12061 ctcttagctc aaccatatgg tcgatatagg ctttatagtc agataaatca tggtcgcaca
    12121 aaataactgt ctttccatca agagccaact cttttagaat ctccaatatc tcgattctgc
    12181 tcttgcggtc aatggaagca aaaggttcat ccaagagata gaccctagga ttcatggcaa
    12241 agaggacagc cagcgctgct ttttgctttt ccccacctga taggtgatga atgggacggt
    12301 gtaagattgc ctcgcaacga cattgctgga caacctctgc tattttagaa tcaatttcct
    12361 gaaggtgata gccgatattc tccaaggtga aaatcagctc ctcaaacaag ctctccatgg
    12421 taaattgatg attgggattt tgaaagagaa tgccaacctt ctggacacgt gcgatgatag
    12481 aaagttgact gacctcgctc ccatctatca ggacttgacc actataggga agagaactga
    12541 cttgagcaat gacttgaaaa aggctggatt ttcctgaacc actgctccca actaacaagg
    12601 taaaggcttg cgcatgaaaa gtaaaatcaa acggctcaga gaagattggg gactgaatcg
    12661 ctcgtagttc cagacccatc tatgcttttc ctccagttgc aaactgatga tagagtttga
    12721 caatggcacg aaccaagatg gtacagaaga aataaacaga aataaaacgt accacaagta
    12781 aggaaaggac aaacggaagg gaaaaggcgt agtaacctaa cttaatgtat tcatagacaa
    12841 agctaacaag cgtaatccca atactattag cagttagaga gagccaactt tcatagcgat
    12901 tcttagttac gataaaacca aattcacttc ccaaaccttg aaccaagcca gacaaaagag
    12961 ctcctagacc aaattggcta ccataaagga cttcagcaag cgcagctagc acttctccaa
    13021 tcgttgcact tccgactctc ggaacaaaga tggcagcaat gggcgcagcc atacaccaga
    13081 gaccgaagag gatttcattg gcaaaggcct gcaaaccaag aggtgttaag agtagactga
    13141 gaatattata cacatatcct gaaccaacga aaaccccacc aaaaaagata gacaagaaag
    13201 caagcaagat aacatctttt aactgccatt ttttcaacat aaaaaactcc tttttttaaa
    13261 gaaaagtggg gcactcaaga agaccgacct aaatactttg tatagcagac tgaatttaga
    13321 acagtacaca agaatgctaa aatatttcta gaaattaatt tgaattttct aattgatttg
    13381 ttcgcatctt atttcaatct actatatcat cttcatccag tttcgtaaaa gaaaaaactc
    13441 taattacaga tacgaattag agttcagctt acaagattag acagttcttt tcgacatacg
    13501 aaaaaaacat ttcacatttc ccttcgccag tcttaactgt atcaggttca atgggtatca
    13561 tctcagccta aagcacccca aatgccttta ttatttaatt atgtgattat tataacacac
    13621 attttatact agttcaagaa attgaactgg aaatacagcc ttgcactcac aaagacagca
    13681 gatctttctt ttgcaaaaaa caaatgacct gtttgatgaa ttagctattc aagctgaatc
    13741 tagacatagc tttttaaaaa aggaaaatcc tacttactta gaatccaagg atagatatct
    13801 attgttcact catttcccga acagtttttt ctatattttt tgcatacgat attgccgaaa
    13861 tgattgaaac gccgtcaaca ttggtcttca taatgtcttt aatatgtttc gtctgtatcc
    13921 caccaattgc aactaagggc atttgtggca atagttttct catcaattta acaccttcat
    13981 aacctatagc accaccagca tcatcctttg actgggtatc aaatacagga ccaacaccaa
    14041 cataatctac atattcaact tttgattgtt gaaattcttc ctcgtttttt atagaaagac
    14101 caattatttt atctggcatc aattttctaa tttcatcaac accaatatca tcttgaccta
    14161 catgtacgcc atcagcgtca atttccattg ccaaatcgat atcatcatta acgataaacg
    14221 gcacattgta ttttttacaa agttctttaa tttggatagc tagctcaagt ttttctaagc
    14281 cttctaaagc accctcacct ttttctcgaa attgaaataa ggttatacca cctcttaagg
    14341 cttcctcaac gactgtatat agattttttc cttggcaagt agtcgttcca caaataaaat
    14401 atagttttag taattcttta tgaaacatct tacttcactc ttttgaattc ctttacatct
    14461 tcatctgtaa tctcgtataa ggcatttata aattcaactt taaatgtccc aggaagatgt
    14521 ccatttggac gtttttctgc catttctcca gcgatattgt aaaccaacat tgctgtttct
    14581 aatgatttca attcttgacc tttttctagt ccgataaagc ttgctaccac tgcccctaat
    14641 aaacatccag ttccaataac ttttggcatc atggcactac cattatgaat cgttaccact
    14701 tctccattta ccgcaatagc atccacttca cctgttacta ctattggaat attgaacttc
    14761 tcatttgctg ctagagcaat ctcgtcaata ttatctactc ccgcactatc tactccttta
    14821 gatgccacat ctattcctac taaagaggca atctctccag cattccccct aatcgcagct
    14881 agtttgtaat tgttgattaa atcatctgct actttttttc tatattctcc tgctccacag
    14941 gctacaggat ctaaaactac tgggacatta tatttctctg caattttcag agcagcttgg
    15001 tataatttcc aattttcatc tgtcaatgtt cctatgttta ttaataaacc accagcatac
    15061 tttaacaaat cctctaaatc tgctggaaac tcactcatgg ctggtgaggc gcccagtgct
    15121 actaatccat ttgctgtgaa attttttact acatcattgg ttatacaaat gaccaatggt
    15181 gctttttctt ttaataattt taaacttgtc atattgaaat ccttcctttt cactttatac
    15241 gatctactga tttcgattta tttctatctt tcgttaagaa tttttttcat ttacattgaa
    15301 tgatttatac tcaatgaaaa tcaaagagca aactaggaag ctaaccgcag attgctcaaa
    15361 acactgcttt gaacctgtag ataagactga cgaagtcagc tcaaaacact gttttgaggt
    15421 tgtggataga actgacgaag tcagtaacca tacctacggc aaggtgaaac tgacgtggtt
    15481 tgaagagatt ttcgaagagt cttacctcat caaatttgta aatatcatga gccttctcta
    15541 gacatcgtaa ccaatatcaa aaaaagctaa ttctaaagcg actgcttgat tccatcgttg
    15601 ctgaagttct gtcaaatctt ctcgattttt accgacacga ttgagttcgt caaccagaaa
    15661 ttgaacccac tctgcaaaga aaggacctct gtggagattg atccattccg aatgaatata
    15721 gacttcaggt agagccaaat ctttagaacc ccagtctaaa tagagacctt ctgcaatgac
    15781 cagcataacc aaaagatggg catagtctga tgaagccact gcccaataca ttagatcctg
    15841 aaaggctttt gttacagggt gcaaagtcac ttctagatag tcattctctg ctacttttaa
    15901 ctctttaaaa gccttttgga aataaccatc ttcatctgct tcaagaaagc ctagttgctt
    15961 ggcaaaacga agcttggatt caagtttatc tgcgtgagct acgcaggcac ccagcatgga
    16021 taagaaggca tcaaagaagt gataatcttg aatcagatag tcctttaaga ccttattctc
    16081 aattgtcccc gcaaaaagtt ccttaacaaa acgatgattg attgcagcct gccaatcctt
    16141 ctgactgctt tttaataatt ctccaacagt caaacctggc tgaaatgcat agtcttgtgt
    16201 ttccatattt acttctcctc tctttacttg ttagtaatta ataaaacacc aagaaatatc
    16261 aagcaaaatc gtaattccac ttgatccttt taaaacacat cgagagtatt tgcagagagc
    16321 taactaaaca agcctatcca gtttatataa acaaaaaact ccaattacaa tcaagaatta
    16381 gagttgactt acaagattag accgttcatt tcaccatacg aaaaaactgt tcacatttcc
    16441 cttcgccagt cttaactgta tcaggttcaa tgggtattat ctcagcctaa agcaccccaa
    16501 atgtctctat tatttaatta ctgaaccagt atagcaaaaa atgaaagcct tagcaagata
    16561 tttaaccgaa aaatatct
//
LOCUS       DAVKHZ010000047        16306 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000047 DAVKHZ010000000
VERSION     DAVKHZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.047"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            397..744
                     /gene="ssrA"
                     /locus_tag="VWW99_001391"
     tmRNA           397..744
                     /gene="ssrA"
                     /locus_tag="VWW99_001391"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(927..1014)
                     /locus_tag="VWW99_001392"
     tRNA            complement(927..1014)
                     /locus_tag="VWW99_001392"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(978..980),aa:Ser,seq:gga)
     gene            1177..1917
                     /locus_tag="VWW99_001393"
     CDS             1177..1917
                     /locus_tag="VWW99_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9539221.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1914..2825
                     /gene="pfkB"
                     /locus_tag="VWW99_001394"
     CDS             1914..2825
                     /gene="pfkB"
                     /locus_tag="VWW99_001394"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9539222.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2822..4774
                     /locus_tag="VWW99_001395"
     CDS             2822..4774
                     /locus_tag="VWW99_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9539223.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4938..7163
                     /locus_tag="VWW99_001396"
     CDS             4938..7163
                     /locus_tag="VWW99_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9539224.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7208..7636)
                     /locus_tag="VWW99_001397"
     CDS             complement(7208..7636)
                     /locus_tag="VWW99_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539225.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7774..8916
                     /locus_tag="VWW99_001398"
     CDS             7774..8916
                     /locus_tag="VWW99_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9539226.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGI
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8925..10139
                     /gene="thiI"
                     /locus_tag="VWW99_001399"
     CDS             8925..10139
                     /gene="thiI"
                     /locus_tag="VWW99_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9539227.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10274..11098
                     /locus_tag="VWW99_001400"
     CDS             10274..11098
                     /locus_tag="VWW99_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9539228.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            11120..11859
                     /locus_tag="VWW99_001401"
                     /pseudo
     CDS             11120..11859
                     /locus_tag="VWW99_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11872..13038
                     /locus_tag="VWW99_001402"
     CDS             11872..13038
                     /locus_tag="VWW99_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9539229.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            13546..15039
                     /locus_tag="VWW99_001403"
     CDS             13546..15039
                     /locus_tag="VWW99_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9539230.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            15052..15594
                     /locus_tag="VWW99_001404"
     CDS             15052..15594
                     /locus_tag="VWW99_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9539231.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRYSEEVVA"
     gene            15591..>16306
                     /locus_tag="VWW99_001405"
     CDS             15591..>16306
                     /locus_tag="VWW99_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9539232.1"
                     /translation="MKKVKLGEVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSTEIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWRGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNT
                     KNVTKNGFSFDTKQFITKTK"
BASE COUNT         4931 a         2966 c         3599 g         4810 t
ORIGIN      
        1 gttagaaatc gatttgactg tcctgatcta ttcgtactat tcttatttca ttttactata
       61 ttttggtgca ataagtgaaa agtagtccga ataatataag gattgatttt atagttttta
      121 aactcaaatg aattaaaata aagagagtac gaaaattctc atctgaaagt attttagaat
      181 aattctcttc gtgaatttct tcaaaacaga tagcttcatc ttaggtatgt gttaattgga
      241 tttcgtaagc tctatttaca atcgtaaagt tatgcactga gaaagttgta gttagtcttc
      301 cagtttgcta aatttctcga atagtatctt ttttaggtat gtgatttctt tttgcatttt
      361 tgagttagat aaggtataat ggttttattg tcttttgggg tcgttacgga ttcgacaggc
      421 attatgaggc atattttgcg actcgtgtgg cgacgtaaac gctcagttaa atataactgc
      481 aaaaaataac acttcttacg ctctagctgc ctaaaaacca gcaggcgtga cccgatttgg
      541 attgctcgtg ttcaatgaca ggtcttatta ttagcgagat acgattaagc cttgtctagc
      601 ggcttgataa gagattgata gactcgcagt ttctagactt gagttatgtg tcgaggggtt
      661 gttaaaataa tacataacct atggttgtag acaaatatgt tggcaggtgt ttggacgtgg
      721 gttcgactcc caccggctcc attattcctt tgcattcttt tgcattcctt ggtaaaacgt
      781 tgttaaatca acgtttttta tttttatctt tggtattcct ttgcattctt ttgctaaaaa
      841 gggagtcaca aacagaccct attttaaaaa aaggatagaa aaaaggatac aacatttgtc
      901 gcatcctaga aataatcttt tttcgacgga agacatggga ttcgaaccca cgcacgctat
      961 tacacgccta ccgcgtttcc aacacggcct cttaagcctc ttgagtaatc ttccaatact
     1021 tactcaaata gtctaccata aaggctctta tcttgcaata aaaattctag aaataagaaa
     1081 aaatgataga ttttgaaaga aaatgataaa aaatgcttga cttcgaaaga aagtatgata
     1141 gaatgaatag tgtaaacgat aacaggaggt gattcagtgt taaaaacaga acgtaaacaa
     1201 ctaattttag aggagttaaa tcaacatcat gtagtttctc tagaaaaatt agttagtttg
     1261 ctagaaacgt cagaatcaac ggttcgaaga gacttggatg agttggaagc ggaaaacaag
     1321 cttcgtcgtg tgcatggtgg agcagaactc ccccactcct tacaggaaga agaaaccatt
     1381 caagaaaaat ctgtcaaaaa ccttcaagaa aagaagttgc tggctcagaa agcagcctct
     1441 ctcattaaag aaaaagatgt catctttatc gatgctggaa caacaactgc ttttttgatt
     1501 catgaattgg tcaataagaa tgttacagtt gtgaccaact ccattcacca tgccgctcag
     1561 ttggttgaaa agcagattcc aactgtcatg gttggaggaa acgtcaagac ggcgacagat
     1621 gctagtatcg ggggcgttgc tcttaaccag attaaccaat tgcactttga ccgtgccttt
     1681 atcggaatga atggtgttga cgatggctat tatacgactc ctgatatgga ggaaggagct
     1741 gtgaaaagag ctattttgga gaatgccaag cagacctacg tcttggtgga ttcgtcaaaa
     1801 attggacaaa cttgctttgc caaggtagca ccactcaaac gcgctatcgt tatcactagt
     1861 caagggcatg agctcttgca ggttattaag gagaaaacgg aggtaataga agtatgattt
     1921 atacagtcac actcaatcca tccattgact atatcgttcg tttggaccaa gtcaaagttg
     1981 gtagtgtaaa tcgtatggac agtgatgata agtttgctgg tgggaaagga atcaatgtca
     2041 gccgtgtctt gaaacgtttg aatataccaa atacagcgac gggatttatc ggtggcttta
     2101 ctggtaaatt tatcacagat actttagcag aggaagaaat cgagacacgt tttgtccagg
     2161 tggcagaaga tactcgtatc aatgttaaaa tcaaagcaga ccaagaaaca gaaatcaacg
     2221 gaacgggtcc aactgttgaa ccggttcagc tagaagaatt gaaagctatt ttatctagtc
     2281 tgacagcaga agatacagtt gtctttgcag gttcaagtgc taaaaatcta ggcaatgtta
     2341 tctataagga tttgatttcc ttgacgcgcc agactggtgc gcaagtggtc tgtgactttg
     2401 aaggacagac cttaattgat agtttggact accagcctct tcttgtaaaa ccaaacaatc
     2461 atgaacttgg agcgattttt ggggttaaac tcgaaagttt agatgaaatt gagaaatacg
     2521 ctcgtgagtt actggctaag ggtgctcaaa atgttattat ctctatggct ggtgatggcg
     2581 cccttcttgt cacatctgag ggagcttact tcgctaaacc aatcaaagga acagtcaaaa
     2641 attcagttgg agctggtgat tctatggttg ctggattcac aggtgaattt gtcaaatcaa
     2701 aagacgcagt agaagccttc aaatggggag tggcttgcgg aacggcaact accttctcag
     2761 atgacttggc aacggcggaa tttattaaag aaacatatgg aaaagttgag gtagaaaaaa
     2821 gatgaaaatt caagacctat tgagaaaaga tgtcatgttg ctagatttgc aggcaactga
     2881 aaaaacagct gtcatcgacg agatgattaa aaatttgaca gagcacagtt atgtaacaga
     2941 ttttgaaaca tttaaagaag gaattttggc gcgtgaagct ttgacttcta ctggtttggg
     3001 tgatggaatc gcaatgcctc acagcaaaaa cgctgctgtc aaagaagcga cagttctatt
     3061 tgctaagtca aataagggtg ttgactacga gagcttggat ggacaagcaa ctgacctctt
     3121 cttcatgatt gcagctccag aaggtgccaa tgatactcac ttggcagcct tggcagaatt
     3181 gtctcaatac ttgatgaaag acggttttgc agacaaactt cgtcaagcaa catctgcaga
     3241 ccaagttatc gaactttttg accaagcttc agaaaaaact gaggaacttg ttcaagtacc
     3301 tgctaatgac tctggtgact ttatcgtagc tgttacagct tgtacaacag gtattgccca
     3361 cacttacatg gcccaagaag cccttcaaaa agtagctgct gaaatggggg ttggtatcaa
     3421 ggtcgaaacc aacggtgcta gcggtgttgg aaatcaacta actgcagaag atatccgtaa
     3481 ggctaaagct attatcattg cagcagacaa ggccgttgaa atggatcgat ttgatggaaa
     3541 accattgatc aatcgtccag ttgctgacgg tatccgtaag acagaagagc taattaactt
     3601 ggctctttca ggagatactg aagtctaccg tgccgctaat ggtgcaaaag ctgcaacagc
     3661 ctctaacgaa aaacaaagcc ttggtggtgc cttctacaaa cacttgatga gtggtgtatc
     3721 tcaaatgtta ccattcgtta tcggtggtgg tatcatgatt gcccttgcct tcttgattga
     3781 cggtgctttg ggtgttccaa atgaaaacct tggcaatctt ggttcttacc atgagttagc
     3841 ttctatgttc atgaaaattg gtggagctgc ctttggtttg atgcttccag tctttgcggg
     3901 ttatgttgcc tactctattg ctgaaaaacc aggtttggta gcaggtttcg tggctggtgc
     3961 tattgccaaa gaaggttttg cctttggtaa aattccttat gccgcaggtg gtgaagcaac
     4021 ttcaactctt gcaggtgtct catctggttt cctaggtgcc cttgttggtg gatttatcgc
     4081 aggtgccttg gttcttgcca tcaagaaata cgttaaagtt cctcgttcac tcgaaggtgc
     4141 taaatcaatc cttctattgc cacttcttgg aacaatcttg acaggatttg ttatgctagc
     4201 tgtgaatatc ccaatggcag caatcaacac tgctatgaat gacttcctag gcggtcttgg
     4261 aggaggttca gctgtccttc ttggtatcgt ccttggtgga atgatggctg ttgacatggg
     4321 tggaccagtt aataaagcag cttatgtctt tggtacaggt acgcttgcag caactgtttc
     4381 ttcaggtggt tctgtagcca tggcagcagt tatggctgga ggaatggtgc caccacttgc
     4441 aatctttgtc gcaactcttc ttttcaaaga taaatttact aaggaagaac gtaactctgg
     4501 tttgacaaac atcatcatgg gcttgtcatt tatcactgag ggagcgattc catttggtgc
     4561 cgctgaccca gctcgtgcga ttccaagctt catccttggt tcagcagtag caggtggact
     4621 cgttggtctt actggtatca aactcatggc gccacacgga ggaatcttcg ttatcgccct
     4681 tacttcaaat gctctccttt acctcgtttc tgtcttggta ggagcaatcg taagtggtgt
     4741 ggtttatggt tacctacgca aaccacaagc ataaaaaata gaaaaatgaa aagattggac
     4801 cgtttggtgc agtctttttc tcttcccgaa atgcctgtga aatatggtat aatagaagaa
     4861 tggcaaacaa gaatacaagt acaacaagac ggagaccgtc taaagcagaa ctggaaagaa
     4921 aagaagcgat tcaacgaatg ttgatttcgt taggaattgc gattttattg attttcgcag
     4981 ccttcaaatt aggggctgca ggtataaccc tttataattt aattcgcttg ctagtgggta
     5041 gcctagctta tctggcgata ttcggcctat taatctatct cttctttttc aagtggatac
     5101 gaaaacagga aggactctta tctggctttt tcaccatatt tgctggctta ctcttgattt
     5161 ttgaggccta cttggtttgg aaatatggtt tggacaagtc cgttctaaaa gggaccatgg
     5221 ctcaggttgt gacagatctg actggttttc gaacgactag ctttgctgga gggggcttga
     5281 tcggggtcgc tctttatatt ccaacagcct ttctcttttc aaatatcgga acttacttta
     5341 ttggttctat cttgatttta gtgggttctc tcctagtcag cccttggtct gtttacgata
     5401 ttgctgaatt tttcagtaga ggctttgcca aatggtggga agggcacgag cgtcgaaaag
     5461 aggaacgctt tgtcaaacaa gaagaaaaag ctcgccaaaa ggctgagaaa gaggctagat
     5521 tagaacaaga agagactgaa aaagccttac tcgatttgcc tcctgttgat atggaaacgg
     5581 gtgaaattct gacagaggaa gctgttcaaa atcttccacc tattccagaa gaaaagtggg
     5641 tggaaccaga aatcatcctg cctcaagctg aacttaaatt ccctgaacag gaagatgact
     5701 cagatgacca agatgttcag gtcgattttt cagccaaaga agcccttgaa tacaaacttc
     5761 caagcttaca actctttgca ccagataaac caaaagatca gtctaaagag aagaaaattg
     5821 tcagagaaaa tatcaaaatc ttagaagcaa cctttgctag ctttggtatt aaggtaacag
     5881 ttgaacgggc cgaaattggg ccatcagtga ccaagtatga agtcaagccg gctgttggtg
     5941 taagggtcaa ccgcatttcc aatctatcag atgacctcgc tctagccttg gctgccaaag
     6001 atgtccggat tgaagcacca atccctggga aatccctaat cggaattgaa gtgcccaact
     6061 ccgatattgc cactgtatct ttccgagaac tatgggaaca atcgcaaacg aaagcagaaa
     6121 atttcttgga aattccttta gggaaggctg ttaatggaac cgcaagagct tttgaccttt
     6181 ctaaaatgcc ccacttgcta gttgcaggtt caacgggttc agggaagtca gtagcagtta
     6241 acggcattat tgctagcatt ctcatgaagg cgagaccaga tcaagttaaa tttatgatgg
     6301 tcgatcccaa gatggttgag ttatctgttt acaatgatat tccccacctc ttgattccag
     6361 tcgtgaccaa tccacgcaaa gccagcaagg ctctgcaaaa ggttgtggat gaaatggaaa
     6421 accgttatga actctttgcc aaggtgggag ttcggaatat tgcaggtttt aatgccaagg
     6481 tagaagagtt caattcccag tctgagtaca agcaaattcc gctaccattc attgtcgtga
     6541 ttgtggatga gttggctgac ctcatgatgg tggccagcaa ggaagtggaa gatgctatca
     6601 tccgtcttgg gcagaaggcg cgtgctgcag gtatccacat gattcttgca actcagcgtc
     6661 catctgttga tgtcatctct ggtttgatta aggccaatgt tccatctcgt gtagcatttg
     6721 cggtttcatc aggaacagac tcccgtacga ttttggatga aaatggagca gaaaaacttc
     6781 ttggtcgagg agacatgctc tttaaaccga ttgatgaaaa tcatccagtt cgtctccaag
     6841 gctcctttat ctcggatgac gatgttgagc gcattgtgaa cttcatcaag actcaggcag
     6901 atgcagacta cgatgagagt tttgatccag gtgaggtttc tgaaaatgaa ggagaatttt
     6961 cggatggaga tgctggtggt gatccgcttt ttgaagaagc taagtctttg gttatcgaaa
     7021 cacagaaagc cagtgcgtct atgattcagc gtcgtttatc agttggattt aaccgtgcga
     7081 cccgtctcat ggaagaactg gagatagcag gtgtcatcgg tccagctgaa ggtaccaaac
     7141 ctcgaaaagt gttacaacaa taaaaaaata gcttctttcc aagtttggag ggaagctatt
     7201 ttagtggcta ttgattgctt ttattttctg aagttggcgc attggactgt ttttcgtttt
     7261 cagtagcagg tttacttgaa gcaggagtag aagagtcctg agttgctgtt ttctgatctt
     7321 cttttttctc ttccttgacg ctagattttg gtgtttcctc ttgctgtgtt ttttcttgac
     7381 tagtgttagt ctctttagtt ggactggtgt tttccttagg ggattccttt tggatttctt
     7441 tgacaatggt tgtcgtctgg cttgtcgtag gttctttttt aatatttttg ttattatcca
     7501 aggcgttcat aatcgtgata cttgcggtaa tcaagccaag gatactagcg attgtagcaa
     7561 cggttttttg aaagactgta aagccttttt tgatgtgttc tgtttttggt tttgaagttc
     7621 tacgatagtt agacatacat tctctccttt gattatgatt tattatactc tgtttcttta
     7681 aaaaaatctt aaaaaaagag aaatttccct ttcttgtcgc aagctccgtt tcatgataca
     7741 atagaggaag tgattttaga aagcgtgaga aacatgattt actttgataa ttcagcgacg
     7801 accaagcctt atcctgaagc ccttgaaacc tatatgcagg tcgcttcaaa aattttagga
     7861 aacccatcta gtctccatcg tttgggagac caagcaacac gaatcttaga tgcttctcgc
     7921 caacagattg cagatttaat cggtaagaaa agcgatgaaa tcttctttac ctcgggtgga
     7981 acagaagggg ataactggct tatcaagggt gtggcctttg aaaaagctca gtttggcaag
     8041 cacatcattg tttcagccat tgaacatcca gcagtcaaag agtcagccct ctggttgaaa
     8101 agtcaaggat ttgaagtgga ttttgctcca gttgataaga aaggcttggt cgatgttgag
     8161 gcgttagcag gtttgatacg gcatgataca atcctcgttt ccatcatggc tgtgaacaat
     8221 gaaatcggct ctatccaacc tattgaggct atttcagaat tcttggcaga caagccgact
     8281 atttccttcc acgttgatgc ggttcaggcg cttgccaaaa ttccgactga aaagtatctg
     8341 acagaacggg tggattgcgc gactttctct agtcacaagt tccacggggt tcgaggtgtt
     8401 ggctttgtct atatcaaatc tggcaagaag attacacctc ttcttacagg tggtggccag
     8461 gagcgagatt atcgttcgac aactgaaaat gtggcaggga ttgcagcgac agccaaggcc
     8521 ctccgtttgt ctatggaaaa gctagatatc tttaggagca agactgggca gatgaaggca
     8581 gtgattcgcc aagctcttct gaactatccg gatatttttg tcttttcaga tgaggaaaac
     8641 tttgcacctc atattctgac ttttggaatc aaaggtgttc gaggtgaagt catcgttcac
     8701 gcctttgaag actatgatat tttcatctca acaacctcag cttgttcatc taaggcagga
     8761 aaaccagccg gtatcttgat tgccatggga gtggacaaag ataaggccaa gtcagctgtg
     8821 cgtcttagcc tagacttgga aaatgatatg agtcaggtcg agcagttttt gaccaagtta
     8881 aaattgattt acaatcaaac tagaaaagta agataggagc attcatgcag tattcagaaa
     8941 ttatgattcg ctacggagag ttgtcaacca agggtaaaaa ccgtatgcgt ttcatcaata
     9001 aacttcgtaa taatatttcg gacgttttgt ctatctatac ccaagttaag gtaacagcag
     9061 atcgcgaccg tgcccacgct tacctcaatg gagctgatta cacagcagtt gcagaatctc
     9121 tcaaacaagt ttttggaatt caaaactttt ctcctgttta taaggttgaa aaatctgtag
     9181 aagttttgaa gtcttctgtc caagagatta tgcgggacat ctacaaggaa ggtatgacct
     9241 ttaagatttc tagcaagcgt agcgaccaca actttgaact tgatagtcgt gaactcaacc
     9301 aaacacttgg aggggctgta ttcgaagcca ttccaaatgt gcaagttcaa atgaaaagtc
     9361 ctgacatcaa tcttcaggtg gagattcgtg aagaagcagc ctatctttct tatgaaacca
     9421 ttcgtggggc tggtggtttg ccagttggaa cttcaggtaa agggatgctc atgttgtcag
     9481 gagggattga ctcacctgta gcaggttatc ttgctcttaa gcgtggggtg gatatcgagg
     9541 cagttcactt tgctagtcca ccatatacta gtcctggtgc cctcaagaaa gcgcaggact
     9601 tgacccgtaa attgaccaag tttggcggaa atatccagtt tatcgaggtg cctttcacag
     9661 agattcaaga ggaaatcaaa gccaaagcgc cagaagctta tttgatgact ctaactcgtc
     9721 gctttatgat gcggattact gaccgtattc gtgaggtacg aaatggtttg gttatcatca
     9781 atggggaaag tctaggtcaa gtagccagcc aaacccttga aagtatgaag gctatcaatg
     9841 ctgttaccaa cactcccatc attcgtcctg tggttaccat ggacaagttg gaaatcattg
     9901 acatcgccca ggaaatcgat acctttgaca tttcaatcca accgtttgaa gactgttgta
     9961 ccatttttgc accagatcgt ccaaaaacaa atcctaaaat taagaatgcg gagcagtacg
    10021 aagcgcgtat ggatgttgaa ggcttggttg agcgagcagt ggctggaatc atgattactg
    10081 aaatcacacc tcaagccgaa aaagatgaag ttgatgactt gattgacaat ctgctctaat
    10141 tcagaaaatc caaaagaata gtgaaaatca gtaaaaaaag ttagtttttt ctctaaaaac
    10201 aggtaaaaaa ctaacttttt ttatttttat gatataatga tataaaattt tgaatataga
    10261 gagttttctg acaatgaatc aatcctactt ttatctaaaa atgaaagaac acaaactcaa
    10321 ggttccttat acaggtaagg agcgccgtgt acgtattctt cttcctaaag attatgagaa
    10381 agatacagac cgttcctatc ctgttgtata ctttcatgac gggcaaaatg tttttaatag
    10441 caaagagtct ttcattggac attcatggaa gattatccca gctatcaaac gaaatccgga
    10501 tatcagtcgc atgattgtcg ttgctattga caatgatggt atggggcgga tgaatgagta
    10561 tgcggcttgg aagttccaag aatctcctat cccagggcag cagtttggtg gtaagggtgt
    10621 ggagtatgct gagtttgtca tggaggtggt caagcctttt atcgatgaga cctatcgtac
    10681 aaaagcagac tgccagcata cggctatgat tggttcctca ctaggaggca atattaccca
    10741 gtttatcggt ttggaatacc aagaccaaat tggttgcttg ggcgtttttt catctgcaaa
    10801 ctggctccac caagaagcct ttaaccgcta tttcgagtgc cagaaactat cgcctgacca
    10861 gcgcatcttc atctatgtag gaacagaaga agcagatgat acagacaaga ccttgatgga
    10921 tggcaatatc aaacaagcct atatcgactc gtcgctttgc tattaccatg atttgatagc
    10981 agggggagta catctggata atcttgtgct aaaagttcag tctggtgcca tccatagtga
    11041 aatcccttgg tcagaaaatc taccagattg tctgagattt tttgcagaaa aatggtaagt
    11101 taagaaagga aaaaacgaaa tgcatattga acatcttagc cactggagtg gtcatcttaa
    11161 ccgtgaaatg taccttaacc gttatggaca tggtgggatt ccagttgtgg tctttgcttc
    11221 atcaggtggt agtcacaacg aatactatga ttttggcatg attgatgcct gtgcttcctt
    11281 tatcgaggaa ggccttgtcc agttctttac cctatctagt ttggatagtg agagctggtt
    11341 ggctacttgg aaaaatgctc atgaccaagc ggaaatgcac cgtgcctacg aacgttatgt
    11401 gattgaggag gccattcttt tatcaagcac aagacaggtt ggtttgatgg catgatgacg
    11461 acaggttgct ctatgggagc ctatcatgca ctcaatttct tcctccagca tccagatgtc
    11521 tttaccaaag tgattgctct cagtggtgtt tacgacgcac gtttctttgt cggtgattac
    11581 tacaacgatg atgctattta ccaaaactcg ccagtagatt atatttggaa ccaaaacgac
    11641 ggctggttta ttgaccgtta ccgtcaggca gagattgtgc tgtgtacggg gcttggagcc
    11701 tgggaacaag atggtttgcc atccttttac aagctcaaag aagcctttga caagaaacaa
    11761 attccagcct ggtttgctga atggggacat gatgtcgccc atgactggga atggtggcgt
    11821 aaacaaatgc cttatttcct cggtaatctc tatttataaa aggagttacc tatgaattac
    11881 cttgttattt ctccctacta tccacaaaac tttcaacagt ttaccatcga actagctaat
    11941 aaaggcatca cagtcttggg aattggtcaa gagtcttacg agcaattgga tgagcccttg
    12001 cgcaatagct tgaccgagta ttttcgtgtt gataatcttg agaacataga tgaagtcaaa
    12061 cgtgcagttg cttttctctt ttataaacat ggtccaattg gccgcatcga gtctcacaat
    12121 gaatactggc ttgagctaga cgcaacactc agagaacaat tcaatgtttt tggtgccaaa
    12181 ccagaggatc tcaaaaagac gaaatataag tctgaaatga agaaactttt caaaaaagca
    12241 ggtgttcctg tggtacctgg agctgttatc aagacggaag cagatgttga tcaagcagtg
    12301 aaagaaatcg gtcttccaat gattgccaaa cctgataatg gagtgggagc agccgcaacc
    12361 tttaaacttg agacagaaga cgatatcaat cacttcaagc aagaatggga ccattcaacc
    12421 ctttatttct ttgaaaaatt tgtcacttcc agcgaaatct gtacctttga cgggctcgtg
    12481 gacaaggatg gaaagattgt cttctcaaca acctttgact acgcctatac accgcttgac
    12541 ctcatgattt ataagatgga caattcttat tatgtgctca aggatatgga tcctaaactg
    12601 cgcaagtatg gggaagcaat tgtcaaagaa tttggtatga aagaacggtt tttccatatt
    12661 gagttcttcc gtgaggggga cgattatatt accatcgagt acaataaccg ccctgcaggt
    12721 ggttttacca ttgatgttta taactttgct cattccttgg acctttatcg tggctatgca
    12781 gctattgtcg caggagaaga gttcccagcg tcagactttg aaactcagta ttgtttggct
    12841 acttctcgcc gtgcaaatgc tcactatgtt tattcagaag aggatttgct tgccaaatat
    12901 agccagcagt tcaaggttaa aaaagtcatg ccagctgcct tcgcggaact tcaaggagat
    12961 tacctgtata tgctgaccac tccgagtcga caagaaatgg agcagatgat tgcagatttc
    13021 ggacaacgtc aagaataaga actatcggat taaggaaatt aactccctta atccttttgt
    13081 tttgtctgat aaaaaataag agcatcccaa caaggtagct atcataaaac ttgttcgata
    13141 actatttgaa gcaggattag gtggtcagaa attaaatttt aatatttcaa ttgagtcata
    13201 gtattgtgtt tgcgtatcct taaatcagct aaaaggatcc atgacgacac ctatacgata
    13261 tagttttcaa gataccaaac aagtctatta atattcaatg aaaatcaaag agcaaactag
    13321 gaagctagcc gcaggtttct caaaacactg ttttgaggtt gtggatagaa ctgacagagt
    13381 cagtatcata tacctacggc aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt
    13441 ataaaatatt caggtgacgc atagatatag ttaattgaag ctttgtttga aatctgataa
    13501 aataatgata ttactaagtt ttaaaaacta aagaaaaggg aagatatgat tacaggcgaa
    13561 ttaaaaaata aaatcgatca gctgtgggaa attctttgga cagaaggaaa cgcaaatcct
    13621 ttaacaaata ttgaacagtt gacttatctc ttatttatga aagatttgga tagtgtcgag
    13681 cttggacgtg aaagtgatgc tgaatttcta gggattcctt atgagggagt ttttccaaaa
    13741 gataaacctg aataccgttg gtcaactttt aaaaatatag gagatgctca ggaagtttat
    13801 cgtttaatga ctcaggagat ttttccgttt attaaaaatc tcaaggggga tacagatgat
    13861 acagcctttt cacgatatat gcgagaagct atttttcaaa taaataaacc tgctacgctt
    13921 caaaaggcaa tttctatctt agatgttttt ccaactaggg gattagatgt agattttgat
    13981 aatgacaaac aaagtattac tgatatcgga gatatctatg aatatctgtt atcaaaattg
    14041 tcgaccgcag gtaaaaatgg acagttccgt acacctcgtc acatcatcga tatgatggtt
    14101 gagttgatgc aaccgactat caaagatatc atctcagatc ccgctatggg ttctgctggc
    14161 ttcttagtat ctgctagccg ttacttaaag cgtaagaaag atgaatggga aaccaataca
    14221 gataatatca atcattttca taatcagatg tttcatggaa atgatacgga tacgactatg
    14281 ttgagacttg gggcgatgaa catgatgcta catggagtag aaaatccaca aatcagttac
    14341 cttgactcgc tgtctcaaga taatgaagaa gccgataaat atactttggt tttagcaaat
    14401 cctcctttta agggctcact tgactacaat tcaacctcta atgaccttct tgcaaccgta
    14461 aaaaccaaaa aaacagaatt actctttctt tctcttttct tgcgaacttt aaaaccaggt
    14521 ggacgagcag cagttatcgt acctgatggt gtcctttttg gttcgtctaa agctcataaa
    14581 ggaattcgtc aggaaattgt agagaatcat aagcttgatg ctgtaatctc aatgcctagt
    14641 ggtgtgttca agccttatgc tggagtttca actgccattc tcatctttac aaaaactggt
    14701 aatggtggta ctgacaaagt ctggttttac gatatgaaag cggatggttt aagtttggat
    14761 gataagcgac aaccgattag cgacaatgat attccagata ttatcgaacg ctttcatcat
    14821 cttgaaaaag aagcagaacg tcagagaacg gatcaatctt tctttgttcc agttgctgag
    14881 ataaaggaaa atgattatga tttgtctatc aataaatata aagagattga gtatgaaaaa
    14941 gttgagtatg aaccaacaga ggtcatctta aagaaaatca atgatttaga aaaagaaatt
    15001 caagctggct tggctgaatt agaaaaatta ctcaagtaga gaggtggctg tatgaaaaaa
    15061 gtgaagttgg gggaagtctt atctctaaaa aaaggcaaga aagccactgt acttgctgaa
    15121 caaacaactc taagccaacg ttatattcaa atagatgatt taagaaataa taataattta
    15181 aaattcactg aaagtttaaa tatgactgaa gcactcccag atgatattct gatagcatgg
    15241 gatggagcta atgcaggaac agttggttat ggattatcgg gagctgttgg tagtacaatt
    15301 acggtcttaa aaaagaatga gcgatacaaa gaaaaaatta tatcagatta cttgggagtc
    15361 tttttggaaa gtaaatcgca gtatttacga gaacattcaa caggtgcaac aattcctcat
    15421 ttaaacaaga atatattact tgatttacaa ttagaattgc taggtatcga agaacaagag
    15481 aacattatct gtattcttaa tacgattaaa aggcttatta ctaaaagaaa attgcagtta
    15541 gatgaactaa acttgctcgt caaatcccga tattccgagg aggtggttgc atgaagaagg
    15601 tgaaattagg tgaagtggct acttttatca atggctatgc ttttaaacct caagattggt
    15661 catctgaagg gaaagagatt atcagaatac agaatctgac taaaacttca acagagataa
    15721 attattattc gggtacgatt gataaaaaat acattgttga agctggtgat attcttattt
    15781 cgtggtcggg tacattaggg gtttttcagt ggcgtggaag atccgcagtg ttaaatcagc
    15841 acatttttaa agtggttttt gataagatag atatagataa atcgtatttt aaatatgttg
    15901 ttgaaaaagg tttacaagat gctgtaaaac atactcatgg ttccacaatg aaacatttaa
    15961 ctaaaaaata ttttgataat attatagttc catatacaaa tttgggagag caacaaagaa
    16021 ttgcaagtga gctggatttg ttaagcaagc tgattttaag gagacaagaa caacttgaag
    16081 agcttaatct acttgtaaaa tcccgattta acgagatgtt tggggaaaat aaaatatttg
    16141 aaagcattga taacttattt gatattatag atggtgatag gggcaaaaat tatcctaaat
    16201 cagatgagtt gtttagtgag gagtactgtt tatttttaaa tacaaagaat gttactaaaa
    16261 acggattttc attcgataca aagcaattta tcactaaaac aaagga
//
LOCUS       DAVKHZ010000048        16128 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000048 DAVKHZ010000000
VERSION     DAVKHZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.048"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..921
                     /gene="lytB"
                     /locus_tag="VWW99_001406"
     CDS             <1..921
                     /gene="lytB"
                     /locus_tag="VWW99_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU9539233.1"
                     /translation="FKSGGYMAKNETVDGYQLESDGKWLGGKATNENAAYYQVVPVTA
                     NVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFI
                     PYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDDFKLENPFLFKDLT
                     EATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRS
                     KIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASG
                     MNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(982..2637)
                     /gene="pavA"
                     /locus_tag="VWW99_001407"
     CDS             complement(982..2637)
                     /gene="pavA"
                     /locus_tag="VWW99_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9539234.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2742..3239
                     /gene="ybeY"
                     /locus_tag="VWW99_001408"
     CDS             2742..3239
                     /gene="ybeY"
                     /locus_tag="VWW99_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9539235.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3220..3615
                     /locus_tag="VWW99_001409"
     CDS             3220..3615
                     /locus_tag="VWW99_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9539236.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3632..4531
                     /gene="era"
                     /locus_tag="VWW99_001410"
     CDS             3632..4531
                     /gene="era"
                     /locus_tag="VWW99_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9539237.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4579..5403
                     /gene="mutM"
                     /locus_tag="VWW99_001411"
     CDS             4579..5403
                     /gene="mutM"
                     /locus_tag="VWW99_001411"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9539238.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5403..6008
                     /locus_tag="VWW99_001412"
     CDS             5403..6008
                     /locus_tag="VWW99_001412"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9539239.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEAIVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            5995..7194
                     /locus_tag="VWW99_001413"
     CDS             5995..7194
                     /locus_tag="VWW99_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9539240.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILAFYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7194..7343
                     /gene="rpmG"
                     /locus_tag="VWW99_001414"
     CDS             7194..7343
                     /gene="rpmG"
                     /locus_tag="VWW99_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9539241.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7383..7616
                     /gene="secG"
                     /locus_tag="VWW99_001415"
     CDS             7383..7616
                     /gene="secG"
                     /locus_tag="VWW99_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9539242.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7718..10072
                     /gene="rnr"
                     /locus_tag="VWW99_001416"
     CDS             7718..10072
                     /gene="rnr"
                     /locus_tag="VWW99_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9539243.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKSFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10035..10502
                     /gene="smpB"
                     /locus_tag="VWW99_001417"
     CDS             10035..10502
                     /gene="smpB"
                     /locus_tag="VWW99_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9539244.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10518..11378
                     /gene="tehB"
                     /locus_tag="VWW99_001418"
     CDS             10518..11378
                     /gene="tehB"
                     /locus_tag="VWW99_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9539245.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11451..12404
                     /gene="coiA"
                     /locus_tag="VWW99_001419"
     CDS             11451..12404
                     /gene="coiA"
                     /locus_tag="VWW99_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9539246.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12423..14225
                     /gene="pepF"
                     /locus_tag="VWW99_001420"
     CDS             12423..14225
                     /gene="pepF"
                     /locus_tag="VWW99_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9539247.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLNSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSNYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14227..14940
                     /locus_tag="VWW99_001421"
     CDS             14227..14940
                     /locus_tag="VWW99_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9539248.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            15007..15948
                     /gene="prsA"
                     /locus_tag="VWW99_001422"
     CDS             15007..15948
                     /gene="prsA"
                     /locus_tag="VWW99_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9539249.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT         4861 a         3025 c         3623 g         4619 t
ORIGIN      
        1 ttcaaatctg gtggctacat ggcgaaaaat gagacagtag atggttatca gcttgaaagc
       61 gatggtaaat ggcttggagg aaaagctaca aatgaaaatg ctgcttacta tcaagtagtg
      121 cctgttacag ccaatgttta tgattcagat ggtgaaaagc tttcctatat atcgcaaggt
      181 agtgtcgtat ggctagataa ggatagaaaa agtgatgaca agcgcttggc tattactatt
      241 tctggtttgt caggctatat gaaaacagaa gatttacaag cgctagatgc tagtaaggac
      301 tttatccctt attatgagag tgatggccac cgtttttatc actatgtggc tcagaatgct
      361 agtatcccag tagcttctca tctttctgat atggaagtag gcaagaaata ttattcggca
      421 gatggcctgc attttgatga ttttaagctt gagaatccct tccttttcaa agatttaaca
      481 gaggctacaa actacagtgc tgaagaattg gataaggtat ttagtttgct aaacattaac
      541 aatagccttt tggagaacaa gggcgctact tttaaggaag ccgaagaaca ttaccatatc
      601 aatgcccttt atctccttgc ccatagtgcc ctagaaagta actggggaag aagtaaaatt
      661 gccaaagata agaataattt ctttggcatt acagcctatg atacgacccc ttacctttct
      721 gccaagacat ttgatgatgt ggataaggga attttaggtg caaccaagtg gattaaggaa
      781 aattatatag ataggggaag aactttcctt ggaaacaagg cttctggtat gaatgtggaa
      841 tatgcttcag acccttattg gggcgaaaaa attgctagtg tgatgatgaa aatcaatgag
      901 aagctaggtg gcaaagatta gtactataag tgaatatgat ttgagtgaat agtaagttaa
      961 aaatcctgat ttcaagtaaa atcaggattt tttcatggat gcaatttttt tggagtctgg
     1021 tgtgacgcgg agggtctttt gtcctgtgta agtgacaaag ccgggttttc caccagttgg
     1081 tttattgagt tttttgactt caatcatatc tacctgcacc agattcgaca ggcgcccttg
     1141 agagaagtag gcagctaact ctgctgcgtc tgtcttgact gcatcagatg ggtcaagatt
     1201 tcctgagatg acaacatggc ttccaggaat gtccttagca tggaaccaaa gttcctcctt
     1261 gcgggccatt ttaaaggtca attcctcatt ttgaagattg tttcgtccga catagatgat
     1321 ggttttgcca tcgcttgcta gatattgttc tagttttttg cgtttctgga ttttctcccg
     1381 ttgtcttctg cggataaaac ctgtttgaat caattcttca cggatttcag cgatttcttc
     1441 cagtccagct tggttgagga cggtttctac actttccaga tagagaatag tggctttggt
     1501 ttcttcaatc aaatcagtca agtatttgac agcttctttg agtttctgat accgtttaaa
     1561 atagcgttgg gcattctggt tgggagtcag agccttatca agcgcaatca tgataggttg
     1621 gttggtatag tagttgtcta ggataacctg gtcttggtcg ttaggcactt ggtggaggaa
     1681 ggttgtcagc aattctcctt tttgacgaaa ttcttcagcg ttgtctgtcg ccagtaactc
     1741 tttttcctgt tttttgagtt tgtgtcggtt tttctgaagt tcattttcaa cacgacgaat
     1801 cagttcactg gcctgctgtt tgacgcggtc gcgctcagcc ttatccttat agtaggtgtc
     1861 caacaaatca gaaagatttg caaaaggctc tcccacctga tttgcaaaag gaactggact
     1921 gaaggaagtc tcagtcaagc atggcttggt ttcctgattg aaaaaatttc ggaaagcgga
     1981 aagtttttca ctaaccagta tcctttccaa ttcatttgcc gtatcgcgtc ccagaccttg
     2041 aaagaggctt tgaagatttt ttgctgttag ttcttgggtt tgcaggattt caaagagctt
     2101 ttcatccttg atagtaaaag gattgagaga ttctgtactt ggcggagcga tataggtcga
     2161 tcctggaagt aaggtgcggt agctattttg tgaaaaaccg acgtgtttga taacttcgag
     2221 gattttatga ctgcttttat cgaccagtag aatattactg tgtttcccca taatttcgat
     2281 aatcaaggta gcctggatat ggtctccaat ctcgttttta ttggaaactg tcatttccac
     2341 aatacggtca ttttccactt gctcaatcga ctcaatcagg gccccctgca aatactttct
     2401 caaaaccatg ataaaggtag aaggttgggc tggattttca aaagtcgttt gggtcagctg
     2461 aatgcgtcca aaaactggat gggcagaaag gagcaggcga tggctttggc gattgctgcg
     2521 gatttgcaag accaactctt gttcaaaagg ctgattgatt ttctggatgc gaccattcac
     2581 taattcgctt cgcaattcct caactatgtg gtgtaaaaaa aatccgtcaa atgacatcgt
     2641 tctctccttg tgattgtatt ccatagtatt atatcaaaaa ggtagaataa aatcatggaa
     2701 atgtggtata ataaagccaa gtaaagagaa acgagaagca catgtatatt gaaatggtag
     2761 atgaaactgg tcaagtttca aaagaaatgt tgcaacaaac ccaagaaatt ttggaatttg
     2821 cagcccaaaa attaggaaaa gaagacaagg agatggcagt cacttttgtg accaatgagc
     2881 gtagtcatga acttaatctg gagtaccgtg acaccgaccg tccgacagat gtcatcagcc
     2941 ttgagtataa accagaattg gaaattgcct ttgacgaaga ggatttgctt gaaaatccag
     3001 aattggcaga gatgatgtct gagtttgatg cctatattgg ggaattgttc atctctatcg
     3061 ataaggctca tgagcaggcc gaagaatatg gtcacagctt tgagcgtgag atgggcttct
     3121 tggcagtaca cggcttttta catattaacg gctatgatca ctacactccg gaagaagaag
     3181 cggagatgtt cggtttacaa gaagaaattt tgacagccta tggactcaca agacaataaa
     3241 cgaaaatgga aaaatcgtga cctgatatcc agtttagaat ttgccatcac agggattttt
     3301 actgctatca aggaagaacg aaatatgcga aaacatgcag tgacggctct agtggttatc
     3361 cttgcaggtt ttgtttttca ggtgtcacga atcgaatggc tctttctcct attgagcatt
     3421 ttcttggtag tagcctttga gattatcaac tctgctattg aaaatatggt ggatttggcc
     3481 agtcactatc acttttccat gctggctaaa aatgccaagg atatggcggc tggcgcggta
     3541 ttagtggttt ctcttttcgc agccttaaca ggtgcattga tttttctccc acgaatctgg
     3601 gatttattat tttaaacagt aagaggaaat tatgactttt aaatcaggct ttgtagccat
     3661 tttaggacgt cccaatgttg ggaagtcaac ctttttaaat cacgttatgg ggcaaaagat
     3721 tgccatcatg agtgacaagg cgcagacaac gcgcaataaa atcatgggaa tttacacgac
     3781 tgataaggag caaattgtct ttatcgacac accagggatt cacaaaccta aaacagctct
     3841 cggagatttc atggttgagt ctgcctacag tacccttcgc gaagtggaca ccgttctttt
     3901 catggtgcct gctgatgaag cgcgtggtaa gggggacgat atgattatcg agcgtctcaa
     3961 ggctgccaag gttcctgtga ttttggtggt gaataaaatc gataaggtcc atccagacca
     4021 gctcttgtct cagattgatg acttccgtaa tcaaatggac tttaaggaaa ttgttccaat
     4081 ctcagccctt cagggaaata acgtgtctcg tctagtggat attttgagtg aaaatctgga
     4141 tgaaggtttc caatatttcc cgtctgatca aatcacagac catccagaac gtttcttggt
     4201 ttcagaaatg gttcgcgaga aagtcttgca tctaactcgt gaagagattc cgcattctgt
     4261 agcagtagtt gttgactcta tgaaacgaga cgaagagaca gacaaggttc acatccgtgc
     4321 aaccatcatg gttgagcgcg atagccaaaa agggattatc atcggtaaag gtggcgctat
     4381 gcttaagaaa atcggtagca tggcccgtcg tgatatcgaa ctcatgctag gagacaaggt
     4441 cttcctagaa acctgggtca aggtcaagaa aaactggcgc gataaaaagc tagatttggc
     4501 tgactttggc tataatgaaa gagaatacta agtagaggta ggctcatgcc tgcttcttgt
     4561 ttttacagaa ggagacttat gcctgaatta cctgaggttg aaaccgtttg tcgtggctta
     4621 gaaaaattga ttataggaaa gaagatttcg agtatagaaa ttcgctaccc caagatgatt
     4681 aagacggatt tggaagagtt tcaaagggaa ttgcctagtc agattatcga gtcaatggga
     4741 cgtcgtggaa aatatttgct tttttatctg acagacaagg tcttgatttc ccatttgcgg
     4801 atggagggca agtattttta ctatccagac caaggacctg aacgcaagca tgcccatgtt
     4861 ttctttcatt ttgaagatgg tggcacgctt gtttatgagg atgttcgcaa gtttggaacc
     4921 atggaactct tggtgcctga ccttttagac gcctacttta tttctaaaaa attaggtcct
     4981 gaaccaagcg aacaagattt tgatttacag gtctttcaat ctgcccttgc caagtccaaa
     5041 aagcctatca aatcccatct cctagaccag accttggtag ctggacttgg caatatctat
     5101 gtggatgagg ttctctggcg agctcaggtt catccagcta gaccttccca gactttgaca
     5161 gcagaagaag cgactgccat tcatgaccag accattgctg ttttgggcca ggctgttgaa
     5221 aaaggtggct ccaccattcg gacttatacc aatgcctttg gggaagatgg aagcatgcag
     5281 gactttcatc aggtctatga taagactggt caagaatgtg tacgctgtgg taccatcatt
     5341 gagaaaattc aactaggcgg acgtggaacc cacttttgtc caaactgtca aaggagggac
     5401 tgatgggaaa aatcatcgga atcactgggg gaattgcctc aggtaagtca actgtgacaa
     5461 attttctaag acagcaaggc tttcaagcag tggatgccga cgcagtcgtt caccaactac
     5521 agaaacctgg tggtcgtctg tttgaggcta tagtacagca ctttgggcaa gaaatcattc
     5581 ttgaaaacgg agaactcaat cgccctctcc tagctagtct catcttttca aatcctgaag
     5641 agcaaaaatg gtctaatcaa attcaagggg agattatccg tgaggaactg gctactttga
     5701 gagaacagtt ggctcagaca gaagagattt tcttcatgga tattccccta ctttttgagc
     5761 aggactacag cgattggttt gctgagactt ggttggtcta tgtggaccga gatgcccaag
     5821 tagaacgctt aatgaaaagg gaccagttgt ccaaagatga agctgagtct cgtctggcag
     5881 cccagtggcc tttagaaaaa aagaaagatt tggccagcca ggttcttgat aataatggca
     5941 atcagaacca gcttcttaat caagtgcata tccttcttga gggaggtagg caagatgaca
     6001 gagattaact ggaaggataa tctgcgtatt gcctggtttg gtaattttct gacaggagcc
     6061 agtatttctt tggttgtacc ttttatgcct atcttcgtgg aaaatctagg tgtagggagt
     6121 cagcaagtcg ctttttatgc aggcttagca atttctgtct ctgctatttc cgcggcgctc
     6181 ttttctccta tttggggtat tcttgctgac aaatacggcc gaaaacccat gatgattcgg
     6241 gcaggtcttg ctatgactat cactatggga ggcttggcct ttgtcccaaa tatctattgg
     6301 ttaatctttc ttcgtttact aaacggtgta tttgcaggtt ttgttcctaa tgcaacggca
     6361 ctgatagcca gtcaggttcc aaaggagaaa tcaggctctg ccttaggtac tttgtctaca
     6421 ggcgtagttg caggtactct aactggtccc tttattggtg gctttatcgc agaattattt
     6481 ggcattcgta cagttttctt actggttggt agttttctat ttttagctgc tattttgact
     6541 atttgcttta tcaaggaaga ttttcaacca gtagccaagg aaaaggctat tccaacaaag
     6601 gaattattta cctcggttaa atatccctat cttttgctca atctcttttt aaccagtttt
     6661 gtcatccaat tttcagctca atcgatcggc cctattttgg ctttttatgt acgcgactta
     6721 gggcagacag agaatcttct ttttgtctct ggtttgattg tgtccagtat gggcttttcc
     6781 agtatgatga gtgcaggagt catgggcaag ctaggtgaca aggtgggcaa tcatcgtctc
     6841 ttggttgtcg cccagtttta ttcagtcatc atctatctcc tctgtgccaa tgcctctagc
     6901 ccccttcaac taggactcta tcgtttcctc tttggcttgg gaaccggtgc cttgattccc
     6961 ggggttaatg ccctactcag caaaatgact cccaaagccg gcatttcgag ggtctttgcc
     7021 ttcaatcagg tattctttta tctgggaggt gttgttggtc ccatggcagg ttctgcagta
     7081 gcaggtcaat ttggctacca tgctgtcttt tatgcgacaa gcctttgtgt tgcctttagt
     7141 tgtctcttta acctgattca atttcgaaca ttattaaaag taaaggaaat ctagtgcgag
     7201 taaaaattaa tctcaaatgc tcctcttgtg gcagtatcaa ttacctaacc agtaaaaatt
     7261 caaaaaccca tccagacaag attgaggttt taaagtattg tcccaaggaa agaaaagtaa
     7321 ccctacatct tgaatctaag tagattttat ggtaaaataa tagggatttt aaggagtttg
     7381 atatgtataa cctattatta accattttat tagtattatc tgttgtgatt gtgattgcaa
     7441 ttttcatgca accaaccaaa aaccaatcca gcaatgtatt tgatgccagt tcaggtgatt
     7501 tgtttgaacg cagtaaagct cgcggttttg aagctgtaat gcagcgtttg acagggattt
     7561 tagtcttttt ctggctagcc attgccttag cattgacggt attatcaagt agataagaaa
     7621 ataatgggca ggactaggtc tttgcctctt tttattttta aaggatgttt gagaaggttt
     7681 tacagtaaaa gaaaattaaa aaatctagaa agaaaatatg aaagatagaa taaaagaata
     7741 tttacaagac aagggaaagg tgactgttaa tgatttggct caggctttgg gaaaagacag
     7801 ttccaaggat tttcgtgagt tgattaaaac cttgtcctta atggaaagaa agcaccagat
     7861 tcgctttgaa gaagatggta gtctgacatt agaaattaag aaaaaacatg agattaccct
     7921 caaggggatt tttcatgccc ataaaaatgg ctttggcttt gttagtctgg aaggcgagga
     7981 ggacgacctt tttgtaggga aaaatgatgt caactatgct attgatggtg ataccgtcga
     8041 ggtagtgatt aagaaagtcg ctgaccgcaa taagggaaca gcagcagaag ccaaaattat
     8101 tgatatccta gaacacagtt tgacaacagt tgtcgggcaa atcgttctgg atcaggaaaa
     8161 gcctaagtat gctggctata ttcgttcaaa aaatcagaaa atcagtcaac cgatttatgt
     8221 taagaaacca gccctaaagt tagaaggaac agaagttctc aaagtcttta tcgataaata
     8281 cccaagcaag aaacatgatt tctttgtcgc gagtgttctc gatgtagtgg ggcactcaac
     8341 ggatgtcgga attgatgttc ttgaggtctt ggaatcaatg gacattgtat ctgagtttcc
     8401 agaagctgtt gtcaaggaag cagaaagtgt gcctgatgct ccgtctcaaa aggatatgga
     8461 aggtcgtctg gatctaagag atgaaattac ctttaccatt gacggtgcgg atgccaagga
     8521 cttggacgat gcagtgcata tcaaggctct gaaaaatggc aatctggagc ttggggttca
     8581 catcgcagat gtttcctatt atgtgaccga gggttctgcc cttgacaagg aagcccttaa
     8641 ccgtgcgact tctgtttacg tgacagaccg agtggtgcca atgcttccag aacgactatc
     8701 aaatggcatc tgctctctca atccccaagt tgaccgcctg acccagtctg ctattatgga
     8761 gattgataaa catggtcgtg tggtcaacta taccattaca caaacagtta tcaagaccag
     8821 ttttcgtatg acctatagcg atgtcaatga tatcctagct ggcgatgaag aaaagagaaa
     8881 agaataccat aaaattgtac caagtatcga actcatggcc aagcttcatg aaactttaga
     8941 aaacatgcgt gtgaaacgtg gagctctcaa ttttgatacc aatgaagcga agattttagt
     9001 ggataaacaa ggtaagcctg ttgatatcgt tcttcggcag cgtggcattg ccgagcggat
     9061 gattgagtct tttatgttga tggctaatga aacagttgcc gaacatttca gcaagttgga
     9121 tttgcctttt atctatcgaa ttcacgagga gcctaaggct gaaaaggttc agaagtttat
     9181 tgattatgct tcgagttttg gcttgcgtat ttatgggact gccagtgaga ttagtcagga
     9241 ggcacttcaa gacatcatgc gtgctgttga gggagaacct tatgcagatg tattgtccat
     9301 gatgcttctt cgctctatgc agcaggctcg ttattcggag cacaatcacg gccactatgg
     9361 actagctgct gactattata ctcactttac cagtccaatt cgtcgttatc cagaccttct
     9421 tgttcaccgt atgattcggg attacggccg ttctaaggaa atagcagagc attttgaaca
     9481 agtgattcca gagattgcga cccagtcttc caaccgtgaa cgtcgtgcca tagaagctga
     9541 gcgtgaagtc gaagccatga aaaaggctga gtatatggaa gaatacgtgg gtgaagagta
     9601 tgatgcagtt gtatcaagta ttgtcaaatt cggtctcttt gtcgaattgc caaacacagt
     9661 tgaaggcttg attcacatca ctaatctacc tgaattttat catttcaatg agcgtgattt
     9721 gactcttcgt ggagaaaaat caggtatcac tttccgagtg ggtcagcaga tccgtatccg
     9781 tgttgaaaga gcggataaaa tgactggaga gattgatttt tcattcgtac ctagtgagtt
     9841 tgatgtgatt gaaaaaggct tgaaacagtc tagtcgtagt ggcagagggc gtggttcaaa
     9901 tcgtcgttcg gataagaagg aagacaagag aaaatcagga cgctcaaatg ataagcgtaa
     9961 gcattcacaa aaagacaaga agaaaaaagg aaagaaatct ttttacaagg aagtagctaa
    10021 gaaaggagcc aagcatggca aagggcgagg gaaaggtcgt cgcacaaaat aaaaaggcac
    10081 gccacgacta tacaatcgta gatacgctag aggcagggat ggtcctgact ggaactgaaa
    10141 tcaagagtgt acgagctgct cgaattaatc tcaaggatgg ctttgctcaa gtgaaaaatg
    10201 gagaagtttg gctgagcaat gttcatatcg cgccttacga agagggcaat atctggaacc
    10261 aggaaccaga acgtcgtcgt aaactcctgc tccataaaaa gcaaattcaa aaattggaac
    10321 aagagaccaa agggacagga atgaccttag ttccccttaa ggtctatata aaagatggct
    10381 acgctaagct tcttttagga cttgccaaag ggaagcatga ctatgacaaa cgggagtcta
    10441 tcaaacgtcg tgagcaaaat cgagatatcg cgcgtgtgat gaaagctgtt aatcagcgat
    10501 aaaaagagga attgaaaatg gaaaaattag ttgcctataa acgcatgcct ttgtggaata
    10561 aacaaacaat gcctgaagct gttcagcaaa agcacaatac aaaagttggg acttggggga
    10621 aaattactgt cttgaaggga gctctcaagt ttattgaatt gacagaagaa ggggaagttc
    10681 tagctgaaca cctctttgaa gcaggggcag acaatccaat ggcccaacct caagcctggc
    10741 accgagtgga agctgccaca gatgatgtgg aatggtactt ggaattttat tgtaaacctg
    10801 aggattattt tgctaaaaaa tacaatacca atcctgttca ttcagaggtc ctagaggcca
    10861 tgcagacagt gaaacaaggg aaagctttgg atttgggttg tggtcagggg cgtaattctc
    10921 tttttctagc ccagcaagat tttgatgtga cggctgtaga tcaaaatgga ctagctcttg
    10981 aaatcttgca aagcattgtg gagcaggaag atttggacat gcctgttggc ctttacgata
    11041 tcaattcagc tagcattgaa caagaatatg attttatcgt ttcaacagtt gttctcatgt
    11101 ttctacaagc ggaccgcatt ccagctatta ttcaaaatat gcaggagaaa accagtgttg
    11161 gcggttacaa ccttatcgtt tgtgccatgg acacggagga ttatccttgc tcggttaact
    11221 tcccattcac ctttaaagaa ggagaactgg cagactatta caaggattgg gaattggtta
    11281 agtacaatga aaatccaggc catttgcacc gtcgcgatga gaatggcaat cgtattcaac
    11341 tacgctttgc gaccttacta gctaagaaaa tcaagtaaac acacatgaag attaggaatt
    11401 ttcctgatct tttttctttt ttacgaatga tatagaaaag gagggaattc atgtttgttg
    11461 cgagagatgc taggggagaa ttggtaaatg tgttagagga taaacttgag aagcaagcat
    11521 acacctgccc agcttgtgga ggccagctcc atttgcgtca aggaccaagt gtacggacgc
    11581 attttgccca taaatcctta aaagactgtg attttttctt tgaaaatgaa agtccagaac
    11641 acctggccaa taaggaatcc ctctatcact ggttgaaaaa agagacaaag gttcaattag
    11701 agtacccgct ttcagaactt aaacagattg cggatgtatt tgtaaatggc aatctagctc
    11761 tagaagttca gtgtagtccc ttgcctcaga aagtccttaa agagcgaagt gagggctatc
    11821 gtagtcaggg ttaccaagta ctgtggttgc tgggtcaaaa actgtggctc aaggagcgtt
    11881 tgactcgtct acagcaaggt tttctttatt tcagtcaaaa catgggcttt tatgtttggg
    11941 aattagacaa ggaaaaacaa gttttaagac tcaaatacct gatttaccag gatctccgcg
    12001 gtaaactcca ttatcaaatc aaggaatttt cctatggtca aggtagttta ttggaaatat
    12061 tgcgtcttcc ctataagaga caaaaaatat ctcattttac agtttctgag gacaaggaca
    12121 tctgtcgcta tatccggcaa caactttatt atcaaaatct cttttggatg aaagaacaag
    12181 cagaagccta tcaaaaggga gaaaatatcc tgacttatgg actgaaagaa tggtatccac
    12241 aaattcgacc aatagtgggc aaatttttcc agattgaaca agacttgact agctattatc
    12301 agcactttta tacctattac caaaaaaatc ctcaaaatga ttggcaaaag ctttatccac
    12361 cagcctttta tcagcaatat ttcttgaaaa atatggtaga atagaaagga tggaggaatc
    12421 taatggtatt acaaagaaat gaaataaatg aaaaagatac atgggatcta tcaacgatct
    12481 acccaactga ccaggcttgg gaagaagcct taaaagattt aacagaacaa ttggagacag
    12541 tagcccagta tgaaggccat ctcttgaata gtgcggataa cctactagaa atcactgaat
    12601 tttctcttga aatggaacgc cagatagaga agctttacgt ttatgctcat atgaagaatg
    12661 accaggatac acgtgaagct aagtatcaag agtactatgc caaggccatg acactctaca
    12721 gccagttaga ccaagccttt tcattctatg agcctgaatt tatggagatt agcgaaaagc
    12781 agtatgctga ctttttagaa gctcaaccaa agctgcaggt ttatcaacac tattttgaca
    12841 agctcttgca aggcaaggat cacgttcttt cacaacgtga agaagaatta ttggctggag
    12901 ctggagaaat ctttggttca gcaagtgaaa ccttcgctat cttggacaat gcggatattg
    12961 tgttccctta tgtcctagac gatgatggta aagaagttca gctatctcat gggacttaca
    13021 cacgtttgat ggagtctaaa aaacgtgagg ttcgccgtgg tgcctatcaa gctctttatg
    13081 cgacttacga acaattccaa cacacctatg ccaaaacctt gcaaaccaat gttaaggtgc
    13141 aaaattaccg tgctaaagtt cgtaactaca agagtgctcg tcatgcagcc ctcgcagcga
    13201 attttgttcc agaaagtgtt tatgacaatt tggtagcagc agttcgcaag catttgccac
    13261 tcttacatcg ctatcttgag cttcgttcaa aaatcttggg gatttcagat ctcaagatgt
    13321 acgatgtcta cacaccgctt tcatctgttg aatacagttt tacctaccaa gaagccttga
    13381 aaaaagcaga agatgctttg gcagtcttgg gtgaggatta cttgagccgt gtcaaacgtg
    13441 ccttcagcga gcgttggatt gatgtttacg aaaatcaagg caagcgttca ggtgcctact
    13501 ctggtggttc ttacgatacc aatgccttta tgcttctcaa ctggcaggac aatctggaca
    13561 atctctttac tcttgttcat gaaacaggtc acagtatgca ttcaagctat actcgtgaaa
    13621 ctcagcctta tgtttacgga gattactcta tctttttggc tgagattgcc tcaactacca
    13681 atgaaaatat cttgacggag aaattattgg aagaagtgga agacgacgca acacgctttg
    13741 ctattctcaa taacttccta gatggtttcc gtggaacagt tttccgccaa actcaatttg
    13801 ctgagtttga acacgccatt caccaagcag atcaaaatgg ggaggtcttg acaagcgatt
    13861 tcctaaataa actctacgca gacttgaacc aagagtatta tggtttgagt aaggaagaca
    13921 atcctgaaat ccaatacgag tgggctcgca ttccacactt ctactataac tactatgtat
    13981 atcaatattc aactggcttt gcggccgcct cagccttggc tgaaaaaatt gtccatggta
    14041 gtcaagaaga ccgtgaccgc tatatcgact acctcaaggc aggtaagtcg aactatccac
    14101 ttaatgtcat gagaaaagct ggtgttgata tggagaagga agactacctc aacgatgcct
    14161 ttgcagtctt tgaacgccgt ttaaatgagt ttgaagccct tgttgaaaaa ttaggattgg
    14221 cataaaatgg ttgaatcgta tagtaagaat gctaaccata acatgcgtcg tcctgtcgtc
    14281 aaagaagaaa ttgtagactt gatgcgtcag cgtcaaaagc aggtcacagg tttcttgaaa
    14341 gaattggaag actttgcccg caaggaaaat attcctatta ttccccatga aacggttgct
    14401 tatttccgtt ttcttatgga aaccatgcag cctaaaaata ttctggaaat tgggacggct
    14461 atcggttttt cagctctctt gatggctgaa catgcgccaa atgctaagat tacaactatt
    14521 gatcgtaatc cagaaatgat tggttttgcc aaggaaaatt ttgcccagtt tgacagtcgc
    14581 aagcaaatca ctctcctaga gggagatgcg gtggatgtct tatctacact gacagagtct
    14641 tatgatttcg tctttatgga ttctgccaag tctaaataca tcgtctttct gccagaaatc
    14701 ctcaaacatt tggaagttgg tggtgtggtt gtcttggatg atatttttca aggtggtgat
    14761 gttgccaagg atattatgga agtccgtcgt ggtcagcgaa ccatttatcg aggccttcaa
    14821 aaattatttg atgcaacctt agacaatcca gaactcaccg caacattagt gcctttagga
    14881 gatggtattc tcatgcttcg taaaaatgta gcagatgttc aactgtctga aagcgaatga
    14941 ttttcagaaa aatttaagaa aaaatagtaa aatagataga gtaacactta tctcaaagga
    15001 gtagacatga agaaaaaatt attggcaggt gccatcacac tattatcagt agcaacttta
    15061 gcagcttgtt cgaaagggtc agaaggagca gaccttatca gcatgaaagg ggatgtcatc
    15121 acagaacatc aattttatga gcaagtgaaa aacaaccctt cagcccaaca agtcttgtta
    15181 aatatgacca tccaaaaagt ttttgaaaaa caatatggct cagagcttga tgataaagag
    15241 gttgatgata ctattgccga agaaaaaaaa caatatggcg aaaactacca acgtgtcttg
    15301 tcacaagcag gtatgactct tgaaacacgt aaagctcaaa ttcgtacaag taaattagtt
    15361 gagttggcag ttaagaaggt agcagaagct gaattgacag atgaagccta taagaaagcc
    15421 tttgatgagt acactccaga tgtaacggct caaatcatcc gtcttaataa tgaagataag
    15481 gccaaagaag ttctcgaaaa agccaaggca gaaggtgctg attttgctca attagccaaa
    15541 gataattcaa ctgatgaaaa aacaaaagaa aatggtggag aaattacctt tgattctgct
    15601 tcaacagaag tacctgagca agtcaaaaaa gccgctttcg ctttagatgt ggatggtgtt
    15661 tctgatgtga ttacagcaac tggcacacaa gcctacagta gccaatatta cattgtaaaa
    15721 ctcactaaga aaacagaaaa atcatctaat attgatgact acaaagaaaa attaaaaact
    15781 gttatcttga ctcaaaaaca aaatgattca acatttgttc aaagcattat cggaaaagaa
    15841 ttgcaagcag ccaatatcaa ggttaaggac caagccttcc aaaatatctt tacccaatat
    15901 atcggtggtg gagattcaag ctcaagcagt agtacatcaa acgaatagtc caaatcaatg
    15961 agtcagggaa aaaactcgac ttcaggaaaa aatgaagcaa acattcccac aataaaacgc
    16021 atagtacaag gtttgtactg ccccccaaaa aagttagaca attaatttat ccgaaggatt
    16081 tagttctgta ttgcacagag ctaagtcctt ttagttttat cttaattc
//
LOCUS       DAVKHZ010000049        15721 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000049 DAVKHZ010000000
VERSION     DAVKHZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.049"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..1085
                     /locus_tag="VWW99_001423"
     CDS             <1..1085
                     /locus_tag="VWW99_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU9539250.1"
                     /translation="IIRESLLFVLYTNHLYTSEKDNSGYEEIIRSAILKYELDNQNSR
                     IIIKALIRGDEFSKKVQTLDDVLAGVLELELEEKITHPNIVEYYFYYKKPDRLILSPH
                     NHKKEIDSLDIDLGYDITYNPIKCPHILVSGGTGSGKSIFISFLIIELLKRNSTLYIA
                     DPKNSDLGSLSHYLSDKYIATTPNNIARIVRLVVEQMQERYQAMRDNFHYGSNFADHG
                     FKPVWLIFDEMGAFQASATDKKSKEVITEAMDGIKQIILLGRQAGVFILISAQQMRAE
                     TLNTDLRDNLGLRIALGANSIEGYRMVFGTATPDKFKSIEEKGAGYLYMQGSGKEKAQ
                     YWESPYLDTTQFDFIKELQLYVTKSK"
     gene            1181..1495
                     /locus_tag="VWW99_001424"
     CDS             1181..1495
                     /locus_tag="VWW99_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539251.1"
                     /translation="MKINDFLKPELLGNKFVAVKGYTEVLDRETNKPVALRLNVSIQD
                     EDSDFFMEMIQIKVNTLSPTATPQLLSDKKTCPVKLSNLTVGQFNGNLWFSCNDVVPI
                     SK"
     gene            1512..2240
                     /locus_tag="VWW99_001425"
     CDS             1512..2240
                     /locus_tag="VWW99_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9539252.1"
                     /translation="MAKLFAISDIHGHLDEFISALSKVDLSDKNNRLILIGDYIDNGL
                     QSFQVISKIIELEEVYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEEL
                     NYIFKSNANNFETGVRNEIKNNIKFNPFINWFKKRYRDKRYYETETQIFVHAGVDEEA
                     GKLWKDLTSSEMFTNKFPTTTGGFFKDIISGHIASWEVAKDKTYQGKIYFDGKNHYFI
                     DGDVYHSKTIPVICYDTVTKSYQY"
     gene            2315..3088
                     /locus_tag="VWW99_001426"
     CDS             2315..3088
                     /locus_tag="VWW99_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9539253.1"
                     /translation="MKTLLIGDLHLKSQLILPIVDRIIQTHNIKRVIFLGDYVDLHGQ
                     TNNIQLYAKDLTFLYSWKIDKEQNGIEVINLIGNHDAYYLLGDQAPFSIQNLEVFFAV
                     QGLLQDLKLQVTYQLDDYLVSHAGFNQIFDPQKWHFSPYTKEHEEELDILAYTIGPMR
                     GGEALGGSPLWAHFRELELLPNQDFPKQIVGHTPKESIDISKNIIGIDTFSLYIDKDN
                     KYQFIGNGDLLVYDQGRLEIISTDWATDQTLKQLPEPQI"
     gene            3260..4054
                     /locus_tag="VWW99_001427"
     CDS             3260..4054
                     /locus_tag="VWW99_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEU9539254.1"
                     /translation="MSYNRKIIRTSSYLEIWEYESPIFSNDTTQIDEDHISLKEKKKR
                     RTFEELTTAEQDERIKRISNTRKNSKWKLQRLIDTNFDDRTSFLTLTTKENIQNRYEF
                     NLIFDKFIKRLNYYIYHSKKRLIKYIAVLERQKRGAWHAHILLFSFPFIPHKELLTIW
                     GLGAVRINKLNNLDDSSNAGRYVAKYMEKGIGQELLESLGKKAFYSSRNLQKPKEIKL
                     FTSESLDNLVKNEDILYESNYISKVFRKGHLIDNKVKYKKIKLSKE"
     gene            4062..4331
                     /locus_tag="VWW99_001428"
     CDS             4062..4331
                     /locus_tag="VWW99_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9539255.1"
                     /translation="MQVILPTEQVQEIQSMITNLLQNEISHFKEDLGLDSPYLNKGQT
                     CKYLGISNNTLDGWIQKGLPVIKIGKTVRFNKNEIDKWLCRKAML"
     gene            4373..5593
                     /locus_tag="VWW99_001429"
     CDS             4373..5593
                     /locus_tag="VWW99_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9539256.1"
                     /translation="MTVTKTNSNTYNLKVYIPKEIRPKMGIKGNHFVKRYKTRKEAKE
                     AELEILTKIHQLENGEDILLSKTNDILFSEFFNNIWWDSYKAGQTTSTTKPPTQVTID
                     NTKTVFRKHILPMFGNYSINFLNQNKQVVLNLMTAKAEEYANFKVIRSYVNSIFDWAE
                     ELEYIESNRLSKTIKRIKATKKLKLQDSKNEEDLYLSSEELQDWFDAFREDLENDKLS
                     LKDYVLFFTTFILSDRKSESYALHWKNIDLANAQIDLRHALDKYKNVKSTKGNKKTIF
                     SIPSYLVSLLSEWKKQQKYELAKFGIMQTSDQLVFTYIDTKGNVNSPLHVDYLNNKMN
                     SVKRRHPKLKHATPHKLRHTGATLAKKAGMSLEAISEALTHSDTITTKTYVNASNIVP
                     LSAGQVAYQHLKNK"
     gene            complement(5689..6081)
                     /gene="rpsI"
                     /locus_tag="VWW99_001430"
     CDS             complement(5689..6081)
                     /gene="rpsI"
                     /locus_tag="VWW99_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9539257.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(6100..6546)
                     /gene="rplM"
                     /locus_tag="VWW99_001431"
     CDS             complement(6100..6546)
                     /gene="rplM"
                     /locus_tag="VWW99_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9539258.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            6903..7220
                     /locus_tag="VWW99_001432"
     CDS             6903..7220
                     /locus_tag="VWW99_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9539259.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(7371..8183)
                     /gene="folK"
                     /locus_tag="VWW99_001433"
     CDS             complement(7371..8183)
                     /gene="folK"
                     /locus_tag="VWW99_001433"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9539260.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(8226..8780)
                     /gene="folE"
                     /locus_tag="VWW99_001434"
     CDS             complement(8226..8780)
                     /gene="folE"
                     /locus_tag="VWW99_001434"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9539261.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(8761..10083)
                     /locus_tag="VWW99_001435"
     CDS             complement(8761..10083)
                     /locus_tag="VWW99_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9539262.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLESIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGCFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADH
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKARNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(10085..11059)
                     /gene="folP"
                     /locus_tag="VWW99_001436"
     CDS             complement(10085..11059)
                     /gene="folP"
                     /locus_tag="VWW99_001436"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9539263.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEEFMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(11154..11861)
                     /locus_tag="VWW99_001437"
     CDS             complement(11154..11861)
                     /locus_tag="VWW99_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539264.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLALFIMGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(11914..13332)
                     /locus_tag="VWW99_001438"
     CDS             complement(11914..13332)
                     /locus_tag="VWW99_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9539265.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            13522..14334
                     /locus_tag="VWW99_001439"
     CDS             13522..14334
                     /locus_tag="VWW99_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9539266.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            14673..15692
                     /gene="adhP"
                     /locus_tag="VWW99_001440"
     CDS             14673..15692
                     /gene="adhP"
                     /locus_tag="VWW99_001440"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9539267.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKGKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         5094 a         3161 c         2887 g         4579 t
ORIGIN      
        1 taattatcag ggaaagctta ctatttgttt tatatacaaa tcatctctac acttcggaaa
       61 aggataattc aggttacgaa gaaattatta gaagtgcgat tttaaaatat gaattagata
      121 atcaaaacag tcgtattatc attaaagcgc ttatcagagg tgacgaattt agtaaaaagg
      181 ttcagacact tgatgatgta ttagctggag ttctagaact tgaactagaa gaaaaaatta
      241 ctcatcctaa tattgttgaa tattactttt attacaagaa acctgataga ctaatattat
      301 caccccataa tcataaaaaa gagatagata gtcttgatat agacttagga tatgatataa
      361 cttataatcc tataaaatgt cctcatattt tagtttcagg tggtacaggt agtggaaaat
      421 ctatatttat atctttctta attattgaac ttcttaaaag aaattcaaca ctatatattg
      481 cagatcctaa gaacagtgat ttaggtagtt tatctcatta tttaagtgat aaatatatag
      541 ctacaactcc caataatatt gctcgtattg tacgtttggt agttgaacaa atgcaggaac
      601 gatatcaagc catgcgtgat aactttcatt acggttcaaa ttttgccgat catggattta
      661 aaccagtatg gcttatattt gatgaaatgg gagcatttca agcttcggca acagataaaa
      721 aatctaaaga agttatcact gaagcaatgg acggtattaa acaaattatt ctactagggc
      781 gacaagcagg agtatttatc cttatatctg ctcaacaaat gagagctgaa actctcaaca
      841 ctgatcttag agataattta ggactaagaa tagctttagg agcaaactca atagagggat
      901 accgcatggt atttggcacg gctacacctg ataagtttaa atctattgaa gaaaagggag
      961 caggatatct ttatatgcag ggctcaggaa aagaaaaagc tcaatattgg gaaagcccct
     1021 atcttgatac aacacaattc gattttataa aagaactaca attatacgta acaaagtcaa
     1081 aataatttca atagatattt ttgcttaaat tcaatagatt ttcagcaaaa atatctattc
     1141 ctcatcgctt aagcgatgac aaaaagaaac gaggtattat atgaaaatta acgattttct
     1201 taaaccagaa ttattaggaa ataaattcgt ggcagtaaaa ggatatacag aagttttgga
     1261 cagagaaaca aataaacctg tagcactacg tcttaatgtt tcaattcaag atgaggattc
     1321 tgatttcttt atggaaatga tccagataaa agttaatacg ttatctccta cggctactcc
     1381 tcaattatta tcagataaaa aaacatgccc cgttaaatta tctaatttaa cagttggaca
     1441 atttaacgga aatttatggt tttcttgtaa cgatgtagtc cctatttcta aataattcgg
     1501 tgatactatt tatggctaaa ctttttgcaa tatctgatat acacggtcac ctagatgagt
     1561 tcataagtgc tctcagtaaa gtagacctat ctgataaaaa caatcgtctt attctaatcg
     1621 gagactatat tgacaatgga cttcaatctt ttcaagtcat ttccaaaata atcgaacttg
     1681 aggaggttta tcctaatcaa attattactc ttctaggaaa ccatgaagaa tggttttatg
     1741 attggttgat tttagataaa cctactgctt cagcatttcc agaaacaata aaaagcttct
     1801 tctctccaga ggaattgaac tatattttta aatcgaatgc taacaacttt gaaacaggtg
     1861 ttagaaatga aattaaaaat aatatcaaat tcaacccatt tataaactgg tttaaaaaac
     1921 gatataggga taaacgctat tatgaaactg aaacccaaat ttttgttcat gctggtgttg
     1981 atgaagaagc gggcaagctt tggaaagacc taacgagttc agagatgttt acaaataaat
     2041 ttcctactac cactggtgga ttttttaaag atattatttc tggtcatatt gcttcttggg
     2101 aagtagctaa agataaaact tatcaaggta agatttactt tgatggtaaa aaccattact
     2161 tcattgacgg cgatgtgtat catagtaaaa caatacctgt tatctgctat gatacagtta
     2221 caaaatccta tcaatattaa ataacatcct aaaaaaattg gtataattta attctattct
     2281 tagactaata tcaaaaataa agaggggaat ctatatgaag acattattaa ttggcgactt
     2341 acatttaaaa tctcaactaa ttttacctat tgttgacagg attatccaaa cacataatat
     2401 taaaagagtc atttttcttg gtgattatgt tgatttacat ggacaaacta acaatatcca
     2461 actatatgcc aaagatctca cttttttata ctcttggaaa atagataaag agcaaaacgg
     2521 aatagaggta attaatctta taggtaatca tgatgcttat tatttactgg gagatcaagc
     2581 tcctttctca attcaaaatt tagaagtgtt tttcgcagtt caaggattgt tgcaggattt
     2641 aaaattacaa gtgacttatc aactagatga ttatttagtc agtcacgctg gtttcaatca
     2701 aatatttgat cctcaaaaat ggcatttttc cccctatacc aaagagcatg aagaagaatt
     2761 agatattcta gcttatacaa taggaccaat gcgtggtggg gaagctcttg ggggttctcc
     2821 tttatgggca cattttagag aattagaact gcttcccaac caagactttc ctaaacaaat
     2881 tgttggacat actccaaaag aatctattga tatttctaaa aatattattg gtatagatac
     2941 tttttcatta tatattgaca aagataataa atatcaattt attggtaacg gagatttgct
     3001 agtttatgat caaggacgtc ttgaaatcat ttcaactgac tgggctacag accaaacatt
     3061 aaaacaatta cctgaacctc aaatttaact actacttctc atcatgtgac taagcgaaac
     3121 tgccgactag aagtcggcaa gctggaagcg tgtttagcag agcgatgctt agaaacacat
     3181 gatagaagtg tggagaagct ttgcttctcc tagtttattt attatataag caaacattac
     3241 gagaaaagga gatgattttg tgagttacaa tcgcaaaatt atccgaactt cttcgtacct
     3301 tgaaatatgg gaatacgaat ctccaatttt ttcaaatgat actactcaaa ttgatgaaga
     3361 ccacatttca ctaaaagaaa agaagaaacg tagaactttt gaagaactca caactgctga
     3421 acaagatgaa cgaataaaaa gaatttctaa cactcgtaaa aattctaaat ggaaacttca
     3481 acgcctaata gatacaaatt ttgatgacag aactagtttc cttaccctta caactaaaga
     3541 aaatatacaa aacagatatg agtttaattt aatttttgat aaatttatta aacgtttaaa
     3601 ctattacata taccattcca aaaaaagact tattaaatat atcgcagtat tagaacgaca
     3661 aaaaagggga gcgtggcatg cacacattct ccttttttca tttcctttta tccctcataa
     3721 agaattactg acaatttggg ggttaggtgc agttcggata aacaaactaa ataacttaga
     3781 tgattcttct aatgctggtc gttatgttgc aaaatatatg gaaaaaggca taggccagga
     3841 attattagag agtctaggaa agaaggcttt ttattcttct cgtaatttac aaaagcctaa
     3901 agaaataaaa ttatttacaa gcgaatcctt agataacctt gttaagaatg aagatatcct
     3961 atatgaaagc aactatatta gcaaagtatt tcgaaaagga cacttaattg acaataaagt
     4021 aaaatacaaa aaaataaagt taagtaagga gtaaggaaaa aatgcaagtt attctaccaa
     4081 cagaacaagt tcaagaaata cagtcgatga ttactaatct cttgcaaaat gaaatttctc
     4141 atttcaaaga agatttaggt ttagatagtc cttaccttaa caaggggcaa acttgcaaat
     4201 atctagggat atctaacaat acattagatg gctggataca aaaggggcta cccgttatta
     4261 aaattggtaa gactgtccgt ttcaataaaa atgagattga taaatggctt tgtagaaaag
     4321 ctatgctata atatctctaa tgatatatcc tgactcgtaa aaggagaaca tcatgacagt
     4381 tacaaaaaca aatagtaaca cttacaatct taaagtgtat atacctaaag aaatccgacc
     4441 taaaatgggg attaaaggaa atcactttgt taaacgctat aaaacaagaa aggaggccaa
     4501 agaagctgaa ctagaaattt taactaagat acatcaactt gaaaatggtg aggatatcct
     4561 actttctaaa actaacgata ttctattctc tgagttcttt aataatattt ggtgggattc
     4621 atacaaagct ggtcaaacaa cttcaacaac taaaccccca acacaagtta ctattgataa
     4681 tactaaaact gttttcagaa agcatatctt accaatgttc ggtaattatt caatcaattt
     4741 tctgaatcag aataagcaag tcgtactaaa cttaatgact gctaaagctg aagagtatgc
     4801 taatttcaag gttattcgaa gctatgtaaa ctcaattttc gattgggctg aggaattaga
     4861 gtacatagaa tcaaatcgat tatccaagac aattaagcga attaaagcta ccaaaaagct
     4921 aaaactccaa gactccaaaa atgaggaaga cctgtatcta tctagtgaag aattacaaga
     4981 ttggtttgat gcttttagag aagatttaga gaacgataaa ttatcactaa aagattatgt
     5041 cctattcttt acaaccttta ttttgagcga tagaaaatca gaatcctatg ctctacattg
     5101 gaaaaacata gacttggcaa atgctcaaat tgacttaagg catgcacttg acaaatataa
     5161 aaatgtgaag tcaacaaaag gaaataagaa aacgattttt tcaattccta gttaccttgt
     5221 atcacttctt tcagaatgga aaaaacaaca gaaatatgag ttagcgaaat ttggtataat
     5281 gcaaacatcc gatcaactag tctttaccta tatagatacg aaaggtaatg taaatagccc
     5341 tctacacgtt gattatctta ataacaaaat gaactccgta aaaagacggc acccaaagtt
     5401 aaaacacgct acacctcata aattacgaca tacaggagcg acacttgcta aaaaggcagg
     5461 aatgagctta gaagccattt ctgaagcatt aactcatagt gatacaatta caactaaaac
     5521 ttatgttaat gcttcaaaca tagttccatt atctgctgga caagttgcct accaacatct
     5581 taaaaacaag tagggtaatt ttagggcaaa tttttttaga aagtatagaa aaaagcaccc
     5641 ataaggcaaa cctttgagtg ctttgaaaca ttgatataat cgtattgatt aacgttttga
     5701 gaattgtgat gctttacgag ctttcttaag acctggtttc ttacgttcaa ctttacgtga
     5761 gtcacgtgta agaagtcctg cgcgtttcaa tgaatcgcgg aagtctgggt ctacttgaag
     5821 aagggcacga gcgataccgt gacggatagc tcctgattga ccagcgtatc caccacctac
     5881 aacgttaacg aaaacgtcgt atgaacctac agttgaagta actgcgaatg gttggttgat
     5941 gacaagacga aggtcagcgt gtgggatgta ctcttcaaca tcttttttgt taacagtgat
     6001 tttaccagtt cctggaacaa ggcgaacgcg tgcaacagcg tttttacgac gtccagtacc
     6061 tgcatattgt gcttgtgaca tactttattg ttcctttcct tagataagtc ctgaaatgtc
     6121 aagaacttct ggttgttgtg cagcgtgagt gtgctcagct ccaacaaata ctttcaactt
     6181 cataccttga gcgcgtccaa gtgtattgtg tggaagcata cctttaactg atttctcaat
     6241 caaacgtact gcatttttag aacgaagttc acctgcagag atttgtttca atccacctgg
     6301 gtggtttgag tgagtgtagt agattttatc agttgctttt ttaccagtca atttaacttt
     6361 ttcagcattg ataacaatca cgaagtcacc tgtatcagtg tgtggtgtaa atgttggttt
     6421 gttttttccg cgaagtacgc tagcaactac tgcagaaaga cgtccaagtg gtacatcagt
     6481 tgcgtcaact acgtaccatt tacgttcaac ttggcctggt ttagccataa atgttgtttt
     6541 gttcatgatt tctcctatat atagttcgat ttttgtttac ggtgatgggt gttccttccc
     6601 atcgaaaagt atttggaagg ttccggggcc tttcaaatgg ggtaaacaat accgcctact
     6661 atcataccaa atttcacccc taaaagtcaa tagatatgaa aaatattttt cggtaacttc
     6721 tcaaagtaac tgaaagttat ataatcaagt taatttggga atttacctat taacttcggt
     6781 agatttttgg gaattttttt ccttcttttt ccctgtagtt aaaatccttt tcaaaaaatc
     6841 accttttctt gatttttaat cctatttgct gtatgataag ggaaaagaaa ggggacagag
     6901 atatggcttt taccaatacc cacatgcggt ctgctagttt tggcattgtt accagcttgc
     6961 ccgatgacat cattgactct ttttggtata tcatcgatca tttcttaaaa aatgtctttg
     7021 aattggaaga agaactcgag tttcaattgc ttaataacca aggaaagatt accttccact
     7081 tttcaagtca acacctccct acagccattg attttgactt taaccatcct ttcgaccctc
     7141 gttatccccc aagagtactg gttttagaca tggacggtag agaaactatc ctcctcccag
     7201 aagaaaatga cctattttaa tactcaatga aaatcaaaga gcaaactagg aagctaaccg
     7261 caagctgtac ttgagtacgg taaggcgacg ctgacgtggt ttgaatttga ttttcgaaga
     7321 gtataaaaac tctaaccttc agttgcaagt gactgaaaac tagagttttt ctattttttc
     7381 aaagcatcat acaagttgcg gatcggttgt tttaatatcg gatggataaa atgaggcgca
     7441 atttcctgta aggactcaag gacaaaaagg cgttccgcta tgtaaggatg aggcaatatg
     7501 aggtcgtctg tataaaggat ctggtcctcc acaaagagca agtccaaatc aatcaaacga
     7561 ggtccccaat gcacttctct cacccgtccc agctctgact caatggctaa caaggtttct
     7621 aacaagtctt gtgctggtag ccaagtttcc acctcaacca cttgattggc aaagctatcc
     7681 tgctccactc caccccaagg ctccgtcgct aagacactgg actctttgag aatatggatg
     7741 ccacgagctc gcagtttgtc aatggcttgc ttcaagtttg cttgtttatc tcccatattg
     7801 cttcctaggg cgataaaggc tcgttgcttg cggcgatgaa tggttaccga gcaagtatct
     7861 agtgacaaat gcaccggtgc ccaaggtttt ttcagttcca acttcatttc ttggacaaga
     7921 ggataagact caaaggtacg ttccaccagt ttataggcta ccgtttcaat caaatcttca
     7981 ctcgtttcct gaaaccaagt cgtccactgc tgacacaatt ctccgtaatg gacagaggct
     8041 gttaaatcca agtctgtagc tgccttggtc atatcatagg ataggatggc tgaaacgaca
     8101 aatttctgcc ccaattcttt ctcactagga aaaagaccat gataggcaaa catttccaaa
     8161 tccttaatct gcagttgatc cataatgatt cctttctaga aaaatccgct tgaagcggat
     8221 tctttttata gccccattaa acgataagct tggtcacgga gatccttatc tgtttcaaat
     8281 agaccacgag ctactgtcgt caaggttgca gtgcctggtt ttctaacacc acgcatactc
     8341 atacacatat gttccgcctc aatgacaaca aaggctcctt tagcacctag atagtccatc
     8401 aaggcatcgg ccacttcgat attcaaacgt tcttgaattt gtggtttttt cgaataaact
     8461 tcaaccgtac gggctagctt agacaagcct gccacacgac catctggaat gtaggcaatg
     8521 tgcgctctac cataaaatgg caagaagtgg tgttcacaca tggtatggaa aaagatatcc
     8581 ttttctacca ccatattatc gtcaataatt tcaaaggatt ttgacaaatg ttcctctgct
     8641 gtttgaccaa gacctgaaaa aatctcttga tacatacggg ctacacgagc aggtgtttcc
     8701 tgcaagccct cgcgattagc gttctctcct acagcctcga taatcatttt tacagctgct
     8761 tcaatctttt gtgtatccat tctcgttctt cctctccatc agataggccc tcacttggct
     8821 caagaaatac aaggaacctg tgacaatcct aactgtttgt ttctcttctt ttttatctgt
     8881 caaattctgc tctagaaaat catgccaatc ttggtagctg agatttctag ccttagctgc
     8941 ctctttcagc acgctttcat ccgtcgcccg actatccgca aaatgtgtta gagtaagctc
     9001 ggtatctggc atggctccca gcaagtccaa catatcctcc aaggctttgg ttttgataca
     9061 agtgaagagg atttccttat gatgatccgc aaaacgttct tgcaaggtta ccaacaaggc
     9121 cttgatagca tggggattgt gggctccatc caaaatcatc aagggatctc ttgacacgat
     9181 ttccaaacgc cctggccaac ttgtttcttc caaggcttga ccaagaaagt cattgcttgc
     9241 tagctctcga ccatcttctt gacaaaaagt atcaagtaaa gctatggcca tcccagcatt
     9301 ctctatttgg tacaaaccaa gcaggctagt ctggaagcaa ccttgtctga cagcacttgt
     9361 atagtcaaag acttcccctg tcaccacact ttcttgatga cgaacctgat aatctgtccc
     9421 gtaggcaagt ctcggcgcat ctttctcttc cgcaatgcgg tcaatcacag ctaaggcttc
     9481 tggagcaata cgccctgtta ccaagggcat cccttgtttg ataatacctg ccttctgctc
     9541 tgctatggac tccaaggtgt caccaagtag ggccacatga tccaagccaa tagttgtaat
     9601 tcctgtcaaa atgggctgac agacattggt actatccaaa agtccaccca tgccaacttc
     9661 catgatggcc acatctactt gctctgaggc aaagtagtca taggccaggg ctgtgataat
     9721 ctcaaactcg gttgtgccct gtaaattggc gaccgcttct ccctccagca aagactgata
     9781 gtctgccatg agagcttcta gcctcgcttc tgagatcgat tccccattga tgctaatctg
     9841 gtctgtgtaa tgaatgagat agggcgagct aaacacgcca actctcaacc ctagcttttc
     9901 tagcatcttt ttcaaaaaag caatagtcga gcccttgccg ttagtccctc cgatatggag
     9961 gaccttgagt ttgagatggg gattgccacg caaagctaac agttccacca ttcgttccaa
    10021 gccaaaatgc ggttgatccg tccggtagtt agcaatccac tgattgtttt caatttcttt
    10081 catcttattt atattgtttt aaatctaaat tttccgcttc atcagccaga cgaatggcag
    10141 aggcaatttc aactgccatc ctgtgactag ctacgtcatg cacgcgcacc acttctacac
    10201 cctgtcttgc agcgatactg gttacatgag ccgaagccgt gtcccgattg cggaaaccaa
    10261 gctctgtctc aggattgact tcaaaaccat tctcctctag gatattgatg acaaatcgct
    10321 tgcgcgacac tccgagaaag attggatagc ccttctgatg tagtttatcc aggtcccgta
    10381 aaagaagcag attttctttc ttggtcagac caaagccaat tcctggatcc aacaggatat
    10441 tttctggtgc aataccagct tctgccgctc tcgctagtgc tcgttcaaag aaagcctcca
    10501 taaattcttc gattggcaat gtttcaaagt cagctaactc tttttctgta aaggtttgac
    10561 caaaaccaaa atgagggaag ataagcgaac taggatgctg aggccgagcc ataactgggt
    10621 taaacatgat gaccactttc gctctcgctt cagctaccac atgagccatt ttctcatcac
    10681 ccataagacc agtgatatca ttgactagat cggcaccagc agccaaagca gcctctgcta
    10741 cttgactctt ccaagtatca atagagatga ggacatcact ttccttgcga atcgctttga
    10801 tcactggaac aacacgctgg atttcctctt ctatctcaac atagctactg cccggccgag
    10861 tcgattctcc gccgatatcg agcatactgg ctccttctgc tatcaattta cgagcctgct
    10921 ggagcgcctg ctcaagagca aaaaattgac caccgtccga aaaggagtct ggggttacat
    10981 tgataattcc gcaaataact gtctttgcat gattggcttt acttgacata tcggtcactc
    11041 cctcaaggat tttcatcata ttatttttct attttaccat aaaaagaaaa agatggacac
    11101 gattgcattc atctttccct agtagaaaga ggtcggttcc tacataaaaa cttttaaaca
    11161 gaaattccta atgtccgact cataatcacc acaagagcca acaaacagaa agcaatccca
    11221 ttaacaatca tgtgaagcaa gatcgacatt tccaaacgtt gggtcttgta ggctgtccaa
    11281 gatagaactg tcgacatacc tccataaatc aataaagaag gtaaattact tggttgatgc
    11341 aataaagcaa acacaatcgt accgactaca aatcccaagt tctccttgcc tcggaaaatc
    11401 tttttaggaa caattccacg gcacaagatt tcctcacaaa ttggagcaag caaggctagc
    11461 aagaagaagc tagaaatcag agaactattc tgaaccatat cgttaatctg agactggtta
    11521 gctgttgtcg tctcatttga cagttgcaac aagatagaac caagtatatt tgaacagata
    11581 atgactaaat aactcaagcc caaacgtgcc aaatccttag ttctaaaaaa agaaaaatta
    11641 aaacttgcca attgcgtttt acgagctccc ataataaata gagccagaac cacaatagaa
    11701 ataccagtaa ctatcagccc tgactgtagc agggctactt cttttaaagt caaaatagag
    11761 gtaaccacta ggggaacctg atataaaagg acggcaagta aaaagaccaa aatccagcct
    11821 gcacgattca acaattcttt ccacatattt ttctctttca tcacctatct cctttgtatt
    11881 aaaaaagggg ggaaatcccc ctgggtcatt ctattataag gccatgctga tgtagttaag
    11941 gataaacaag gcatccaaaa tccaaatcat gacatgaaca tctttaactt gacctttaac
    12001 aagcttagtc aaagtgtaag tcaagaaacc aactgcaatc ccttgagtga tagagtagct
    12061 gaatcccata aagatagatg tgaagaaggc aggaactgct tcagacatat catcccaatg
    12121 gatatttttc aagctaccaa gcatcataat cccaacgata atcaagattg gagctgtagc
    12181 cgctgttggt acgatcgcta gaagtgggct aaagaagctt gaaatcgcaa aacagatagc
    12241 tacaaccaag gctgtcaaac cagtacgtcc acctgcaccg ataccagcag cagactcaac
    12301 ataagtcgtt acgtttgaag tacctgcaat ggcaccgact gtcgttccaa tcaaatcaga
    12361 gtaaagagcc ttatccaatt tggctgattg gtgattttca ccatttgtcg ctacgatacc
    12421 aactttttca cctgtaccga tcaaggtacc aattgtgtca aaaatatctg tcaatgagaa
    12481 ggcaagaatg gccatcagag tttcaggcaa gcgagctgta tctgaaacca aagctcccaa
    12541 accttctgaa ccaagagctg caccaaagat tgtcttcaaa tcttcaaaag ctgcaccaac
    12601 atggttatta gcaaaatcga tactagacaa atcaaccaaa ccaactgcaa tagcaagaac
    12661 agttgttgtc aagatagaga gaataattcc ccctttaatc cctttgatga caaagaagat
    12721 agtaatggca agtcctgcaa gagccactaa aacagctgga ttattaaagc tgaccaatcc
    12781 tggaactgct gaagagtttg ctgcaatcgt tgcttgagct ttgtcagccc cttctcctac
    12841 aacagtatag ttgcctggat caatcgtgaa tttcaaaagt ccagcattct taatccctac
    12901 ataggcaagg aagacaccga taccagctga aatagctgag cgaagagcat tgggaatcga
    12961 ttcaatgatc attttacgaa catttgtcaa ggtaataatc aatgaaataa tcccacagat
    13021 gaagaccata gctagggctt cttgccaaga ataaccaagc ccgaatacaa ctgtaaaggt
    13081 aaagaaggca ttgagtccca tacctggcgc ttgggcataa ggtaagttag cataaaaagc
    13141 catcatcaag gtacccgcta ctgcaccaat aatcgtcgct aggaagacgc cctgagcagg
    13201 cattcctgtt tgtgaaagta tttgtgggtt tacaaagaga atatagctca ttgcaaagaa
    13261 agttgttaaa ccagcgagaa cctctgtacg aacgtctgta ccgttctctt ttagtttaaa
    13321 taatttgtcc atcatcaatc tccttttatt ttttacgaac aataatagaa ttatatcatt
    13381 tatcattcac tttttcaatg tatttgtttg atttgtttaa gaaattaatc caatttcatt
    13441 ttttgtttgg aatctgcttt tctgcctgct ttctgttata atagaagaca tcttatctga
    13501 aaacacacta gaaaggtcca tatgacgaaa aaaattattg cagttgacct ggatggaacc
    13561 ctgctcaact cagacagtca aatttctgac tttaccaaaa gaaccatcaa aaaagttgct
    13621 gaaaaaggcc atcaggttat tattacgaca ggtcgccctt accgtatgtc aaaagatttt
    13681 taccgtgaac tgggcttaga cactcctatg attaacttca acggatccct tactcattta
    13741 ccagaccaag tttgggattt tgaaaagtgt ttgactgtag acaaaaaata tctgctagat
    13801 atggttcaac gttcagagga cattcaagcc gattttatcg ctggagaata tcgtaaaaaa
    13861 ttctacatta caaatcccaa tgaagaaatt gccaatccca aactatttgg tgtagaagct
    13921 ttccagcctg aagatcaatt ccagcctgaa ttggtgacca aggaccctaa ctgtatcctc
    13981 ttgcagacca gagccagtga caaatatgcc ttggcaaaag acatgaacgc cttctaccag
    14041 catcaacttt ctatcaacac ctggggaggt ccgctcaata tccttgaatg taccccaaaa
    14101 ggtgtcaaca aggcctttgc tttggactac ttgctcaaga taatgaatcg tgacaaaaaa
    14161 gatttgattg cctttggaga tgaacacaat gataccgaaa tgctcgcttt tgctgggaag
    14221 ggttatgcca tgaaaaatgc caatccagag ctactccctt atgcagatga gcaaatttcc
    14281 cttaccaacg accaagatgg ggttgccaaa accctacaag acttattctt ataacctata
    14341 ctgatactca atgagaatca aagagcaaac taggaagcta gccgtaggtt gctcaaagca
    14401 ctgctttgag gttgtagatg aagctgacgt ggtttgaaga gattttcgaa gagtatgacc
    14461 agcttgcaag ctggtctttg tgctcttttc acagtctcat caaccagtta atcgattgtt
    14521 ctactttttg tctccaaaac cttggaaaag gctttctttt gtgatatact tcacatataa
    14581 atacaagaga gctcgtcaca ctcgactctg ttgacaaaaa atcaatccag tcccgttgtc
    14641 cctgttctcg gtcaatatca aggaggatag ctatgaaagc tgttgttgta aatccagaaa
    14701 gcactggtgt tgctattgaa gaaaaagtac tccgtccact tgaaactggg gaagcacttg
    14761 tagaagttga atactgtggc gtttgccaca ccgatctcca cgttgctcat ggtgactttg
    14821 gtcaagtccc aggacgtgtt cttgggcacg aaggtatcgg tatcgttaaa gagattgctc
    14881 cagatgtgaa aagccttaaa gtcggtgacc gcgtcagcgt tgcttggttc tttgaaggat
    14941 gtggcacttg cgaatactgt acaactggtc gcgaaaccct ttgccgtaca gtgaaaaatg
    15001 ctggctactc agtagacggt ggtatggctg aacaatgtat cgtaactgct gactatgctg
    15061 tcaaggttcc tgacggactt gatccagccc aagcttcttc tatcacatgt gctggagtaa
    15121 caacctataa agctatcaaa gaagcaaaag ttgaaccagg ccaatgggtt gttctttacg
    15181 gtgctggtgg acttggtaac ctcgctgttc aatacgctaa aaaagtattc aatgctcatg
    15241 ttatcgcagt cgatatcaac aatgacaaac ttgcccttgc aaaagaagta ggcgctgaca
    15301 ttgtgattaa cggcctcgaa gttgaagatg tagctggact cattaaagga aaaactgatg
    15361 gaggagctca ttcagctgtc gtaactgctg tgtctaaagt tgccttcaac caggctgttg
    15421 actccgttcg tgctggtggt cgcgtcgtcg ctgttggtct tccttctgaa atgatggaac
    15481 tcagtatcgt taaaacagtc ctcgatggaa tccaagtcat cggttctctt gtcggaactc
    15541 gtaaagactt agaagaagcc ttccaatttg gtgcagaagg tctggtagcc ccagttgttc
    15601 aaaaacgtcc agtagaagat gctgttgcca ttttcgacga aatggaaaaa ggtcaaatcc
    15661 aaggacgtat ggtactcgac ttcacccact aatctaatag aaacctattt taaaagttgg
    15721 a
//
LOCUS       DAVKHZ010000050        15702 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000050 DAVKHZ010000000
VERSION     DAVKHZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.050"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            223..2010
                     /locus_tag="VWW99_001441"
     CDS             223..2010
                     /locus_tag="VWW99_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9539268.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2007..2483
                     /locus_tag="VWW99_001442"
     CDS             2007..2483
                     /locus_tag="VWW99_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9539269.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2511..3416
                     /locus_tag="VWW99_001443"
     CDS             2511..3416
                     /locus_tag="VWW99_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9539270.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVVVAAPSTPSESGEIEDDEF"
     gene            3403..4218
                     /locus_tag="VWW99_001444"
     CDS             3403..4218
                     /locus_tag="VWW99_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9539271.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4225..4629
                     /locus_tag="VWW99_001445"
     CDS             4225..4629
                     /locus_tag="VWW99_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9539272.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4878..6044
                     /locus_tag="VWW99_001446"
     CDS             4878..6044
                     /locus_tag="VWW99_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9539273.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVSLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6160..7197
                     /locus_tag="VWW99_001447"
     CDS             6160..7197
                     /locus_tag="VWW99_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9539274.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSITPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7404..8258
                     /locus_tag="VWW99_001448"
     CDS             7404..8258
                     /locus_tag="VWW99_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9539275.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEAFAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIVQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8408..8665
                     /locus_tag="VWW99_001449"
     CDS             8408..8665
                     /locus_tag="VWW99_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9539276.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8797..8880)
                     /locus_tag="VWW99_001450"
                     /pseudo
     CDS             complement(8797..8880)
                     /locus_tag="VWW99_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(8914..9006)
                     /locus_tag="VWW99_001451"
                     /pseudo
     CDS             complement(8914..9006)
                     /locus_tag="VWW99_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9042..9337)
                     /locus_tag="VWW99_001452"
                     /pseudo
     CDS             complement(9042..9337)
                     /locus_tag="VWW99_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(9327..9488)
                     /locus_tag="VWW99_001453"
     CDS             complement(9327..9488)
                     /locus_tag="VWW99_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9539277.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            9558..10322
                     /locus_tag="VWW99_001454"
     CDS             9558..10322
                     /locus_tag="VWW99_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9539278.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(10562..10714)
                     /locus_tag="VWW99_001455"
     CDS             complement(10562..10714)
                     /locus_tag="VWW99_001455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539279.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            10932..12311
                     /locus_tag="VWW99_001456"
     CDS             10932..12311
                     /locus_tag="VWW99_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9539280.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12325..12990
                     /locus_tag="VWW99_001457"
     CDS             12325..12990
                     /locus_tag="VWW99_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9539281.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13125..13283
                     /locus_tag="VWW99_001458"
     CDS             13125..13283
                     /locus_tag="VWW99_001458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539282.1"
                     /translation="MKIKDQTRKLATGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            13345..13884
                     /locus_tag="VWW99_001459"
     CDS             13345..13884
                     /locus_tag="VWW99_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9539283.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            13951..14361
                     /locus_tag="VWW99_001460"
     CDS             13951..14361
                     /locus_tag="VWW99_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9539284.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14477..>15702
                     /gene="pavB"
                     /locus_tag="VWW99_001461"
     CDS             14477..>15702
                     /gene="pavB"
                     /locus_tag="VWW99_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9539285.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRA"
BASE COUNT         4401 a         2979 c         3579 g         4743 t
ORIGIN      
        1 ttttatcaat gtattctata caaaattatt ttaggtccta tattccgaat ttaaagagcg
       61 tttgaaatgg gtctaaaaat gtttgaaact aattgaatgt gtgtataata gtaaaaatgc
      121 agtaaatatc ccgacatttc tattgacaat tcaaacagat tggtttataa ttaatataac
      181 aacaaatgaa agcgcaaact ttcgcggtcg gaaggtagtt ttatgacacg atttgagata
      241 cgagatgatt tctatctcga tggaaaatca tttaagattt tatctggtgc cattcattat
      301 tttagggttc ctccagagga ttggtatcat tcgctctata acttgaaggc tcttggtttt
      361 aatacggtag agacttatgt tgcttggaat ttacacgagc ctcgtgaagg tgagtttcat
      421 tttgacggtg atctggattt agagaaattt ctccaaatag cgcaggattt gggtctctac
      481 gcaattgtgc gtccgtctcc atttatctgt gcggaatggg aattcggtgg cttaccagct
      541 tggctcttga ccaagaacat gcgaattcgc tcatccgacc cagcatatat cgaggcagtt
      601 ggtcgctact atgatcagtt attgccaaga ctggtgcctc gtttgttgaa caatggtggc
      661 aatattctca tgatgcaggt tgaaaatgag tatggttctt acggagaaga taaggcttac
      721 ctgagagcga ttcgacagct aatggaagag tgtggtgtaa cctgtcccct ctttacatca
      781 gatggtccat ggcgagctac tctgaaagct ggaaccttaa ttgaagagga cctctttgta
      841 acaggaaact ttggttctaa ggcaccttac aacttttcgc agatgcagga attctttgat
      901 gaacatggta agaaatggcc actcatgtgt atggagttct gggatggttg gttcaatcgc
      961 tggaaagaac cgattatcac acgggatcct aaggaattgg cagatgcagt tcgagaggtt
     1021 ttggaacaag gctctatcaa tctttacatg ttccacggtg gtacaaactt tggtttcatg
     1081 aatggttgct cagctcgagg aactttggac ctgccacaag ttacatctta tgattacgat
     1141 gcccttctgg atgaagaagg aaatccaact gctaaatatc ttgcagtcaa gaagatgatg
     1201 gcaacacatt tttcagagta tccgcagttg gaaccactct acaaagagag tatggagttg
     1261 gatgctattc cactagttga aaaagtttct ttgtttgaaa ccttagatag cttgtcaagt
     1321 cctgtagaaa gtctctatcc tcaaaagatg gaggagctgg gacaaagtta tggctaccta
     1381 ctttatcgaa cagaaacaaa ctgggatgca gaagaagaaa gacttcgtat cattgatggt
     1441 cgagataggg cccagctgta tgtcgatggt cagtgggtta aaactcaata tcagacagag
     1501 attggggaag atatttttta tcaaggtaaa aagaaagggc tatctaggtt agatatcttg
     1561 atagaaaata tggggcgtgt caactatggg cataagttct tagcggatac gcaacgtaag
     1621 ggaattcgga caggggtctg taaggatctg catttcttac taaactggaa acactatcca
     1681 ctcccactag acaatcctga gaaaattgat ttttcaaaag gatggactca aggacaacca
     1741 gccttttacg cttatgactt tacagtcgaa gagccaaaag atacttacct agacttgtct
     1801 gagtttggta agggagttgc ctttgtcaat gggcagaatc taggacgttt ttggaacgtt
     1861 ggcccaactc tctcacttta tatccctcat agctatctca aggaaggtgc caaccgtatc
     1921 attatctttg aaacagaagg tcaatataaa gaagagattc atttaactcg taaacctaca
     1981 ctaaaacata taaaggggga aaacttatga caattgtagg atgccgtatt gatggacgtt
     2041 tgatccacgg acaagtagcc aatctttggg ctggaaaact aaatgtttca cgcattatgg
     2101 ttgtagacga cgaagttgtc aacaacgata ttgaaaagag tggtttgaaa cttgcgacac
     2161 caccaggtgt gaaattgagt attttgccag ttgagaaagc tgcagccaat attcttgctg
     2221 gtaaatacga tagccaacgt ctctttatcg tggctcgtaa accagaccgc ttccttggtt
     2281 tggtagaagc aggtgtacca cttgaaaccc ttaatgttgg gaatatgtct caaacaccag
     2341 aaactcgttc tattacacgt tctatcaacg tagtagacaa ggatgtggaa gacttccaca
     2401 aactggcaga aaaaggtgtt aaacttactg ctcagatggt tccaaatgat ccaatttcag
     2461 actttttgag cttattaaaa taggaaaaaa atttttagga ggtcattgtt atgatacaat
     2521 ggtggcaaat tttacttctc actttgtact cagcttatca aatctgtgat gagttgacga
     2581 tcgtttcatc tgcaggttct cctgtatttg ctggtttcat tactggttta atcatgggag
     2641 atgtgactac tggtttactt atcggtggta acttgcaact gttcgttctt ggggttggta
     2701 ccttcggtgg tgcttctcgt atcgacgcaa cttctggtgc ggttcttgcg acagccttct
     2761 ctgtttcaca aggaattgat gcaccgcttg ccattactac aatcgctgta ccagtagcag
     2821 ctctcttgac ttacttcgac gttcttggtc gtatgactac taccttcttc gctcaccgtg
     2881 tggatgctgc aatcgaacgc tttgactata aaggtattga acgcaactac ttgcttggtg
     2941 cgattccgtg ggctctatct cgtgcccttc cagtcttctt tgcccttgct tttggtggtg
     3001 cctttgtaca atcagtagta gacttcgttg aagcctacaa atgggttgca gatggcttga
     3061 cactcgcagg acgtatgctt ccaggtcttg gatttgcaat cttgcttcgt taccttccag
     3121 ttaaacgtaa ccttcactac cttgctatgg gatttggttt gacagctatg ttgactgttc
     3181 tttactcata tgtaacaggt cttggtggcg ctgttgctgg tatcgtaggt actcttcctg
     3241 ctgaagttgc tgaaaaaatt ggtttcgtga acaacttcaa aggtttgtct atgattggta
     3301 tttctatcgt aggtattttc cttgcagtgc ttcacttcaa aaatagccaa aaagtagttg
     3361 tagcagcacc ttctacacca tcagaaagtg gggaaatcga agatgacgaa ttctaattac
     3421 aaacttacaa aagaagattt taatcaaatc aacaaacgta gcttgtttac tttccaatta
     3481 ggttggaact acgaacgtat gcaagcttct ggttaccttt acatgatctt gcctcagttg
     3541 cgtaaaatgt atggtgatgg aactcctgaa ttgaaagaaa tgatgaaagt tcatactcaa
     3601 ttcttcaata cttcgccatt cttccatacc attatcgctg gttttgacct tgccatggaa
     3661 gaaaaagatg gtgtaggttc aaaagacgcc gttaacggta tcaagacagg tttgatggga
     3721 ccattcgctc ctcttgggga tacaatcttt ggttcacttg tacctgctat catggggtca
     3781 gtcgcagcaa ctatggctat cgctggccaa ccttggggga tcttcctttg gattgcagtt
     3841 gcagtagcgt atgacatctt ccgttggaaa cagttggaat ttgcttacaa agaaggggtt
     3901 aaccttatca acaacatgca aagtaccttg acagctttga ttgacgctgc atctgtactt
     3961 ggtgtcttca tgatgggtgc tcttgtagca acagtgatta actttgaaat ttcttacaag
     4021 ttgccaatcg gtgaaaagat gattgatttc caagacatct tgaaccaaat cttcccacgt
     4081 ttgcttccag caatctttac tgcctttatc ttctggttgc ttggtaagaa aggtatgaac
     4141 tctactaaag ctatcggtat tattatcgta cttgctttgg ctctttctgc ccttggtcac
     4201 tttgcacttg gaatgtaatt ccttatgact aaatcattaa ttttggtgag ccatggtcgc
     4261 ttctgtgagg agcttagagg tagcacagaa atgattatgg gcccacaaga caacatttac
     4321 acagtagctc ttcttccaga agatggccca gaagaattta ctgctaaatt tgaagctgtt
     4381 attgaaggat tggatgattt cctagttttt gcggatcttc tcggtgggac accttgtaat
     4441 gtggtgagtc gcttgatcat ggaaggtcgt gatattgacc tttacgcagg gatgaatctt
     4501 ccaatggtga ttgaatttat caatgcgagc cttacaggcg cagatgcgga ctacaagagc
     4561 cgtgctgcag aaagcattgt gaaagttaat gacctgttag cgggcttcga tgatgacgaa
     4621 gatgaataat actcttcgaa aatctcttca aactacgtca acgtcgcctt gccgtaggta
     4681 tatgttactg acttcgtcag tcttatccgg caacctcaaa gcagtgcttt gagcagcctg
     4741 cggctagttt cctattttag ttggaactcg attcaattca tgtgacaacg tgaaaatcgt
     4801 tagagcattt tatatagaat atacatggga atggagctta ctcccattcc catatttaat
     4861 agaaaaagag gaactcaatg ctacattata caaaagaaga cttgctcgaa ttgggtgcag
     4921 aaatcactac gcgtgaaatc taccaacagc ctgatgtatg gagagaagct tttgaatttt
     4981 atcaagcaaa acgtgaagaa attgcagcct tcctacaaga aatcgctgat aaacatgact
     5041 atattaaggt tatcttgaca ggtgctggga cttctgctta tgtgggagat accttgctac
     5101 cttattttaa ggaagtctat gacgaacgca aatggaattt caatgctatt gcgacaacag
     5161 atatcgttgc caatccagca acctatttga aaaaagatgt ggcaactgtc cttgtgtctt
     5221 ttgctcgtag tgggaactcg cctgaaagtg tggcgactgt tgatttggcc aaatccttgg
     5281 tggatgagct ttatcaagtg acgattactt gtgcagcaga tggtaaattg gctcttcaag
     5341 ctcacggtga tgaccgtaat ctcttgctct tgcaaccagc tgtctctaat gatgctggat
     5401 ttgccatgac ttctagcttt acgtctatga tgttgacaac tctcttggtc tttgatccta
     5461 cagaatttgc tgttaagtct gaacgttttg aagttgtatc tagtcttgcc cgtaaagttt
     5521 tagacaaggc agaagatgtc aaagagctcg ttgatttaga ctttaaccgt gtcatctatc
     5581 taggcgctgg tcctttcttt ggacttgctc atgaagctca gctcaagatt ttggaattaa
     5641 ctgctggtca agttgcgacc atgtatgaaa gcccagttgg cttccgtcac ggtccaaaat
     5701 ctcttatcaa cgacaataca gttgttttgg tctttggtac aacgacagac tatactcgta
     5761 agtacgactt ggacttggtt cgtgaagttg ctggtgacca gattgctcgt cgtgttgtgc
     5821 ttttgagtga tcaagctttt ggtcttgaaa atgtcaaaga agtgtccctt ggttgtggcg
     5881 gtgtcttgaa tgatatttac cgtgtcttcc cttacatcgt ttatgcccaa ctctttgccc
     5941 tattgacttc actcaaggta gaaaataaac cagatacacc gtctcctaca ggtacagtaa
     6001 accgtgtagt acaaggtgtc ataattcacg aatatcaaaa gtaagacagt gtttatgaat
     6061 tcttgacaag aggatttgta aattatcaga taaaccatag attgtcagta cgctttctat
     6121 ggtttgtttg cttgagagaa atagtaaaag gagaacagaa tgaaagcata cacagagcgt
     6181 gtatttggaa atgttgaggg cgaggatgtc ttggcctatc gatttgagac agacggtggc
     6241 taccaacttg aggttatgac ttatggcgcg accatcttgc gctatgtcgc acctgacaag
     6301 gctggaaatt ttgccaatgt tatcttggga tttgatgact ttgatagtta tgtaggcaat
     6361 agtcccaagc atggagcaag tgtaggtcct gtagcgggtc gtattgcagg tgcgaccttt
     6421 gagctcaatg gtaagaccta tgaccttgag gttaataatg ctagcaactg taatcacagt
     6481 ggttcaactg gttgggattc cagcttgttt gaagttgaag aagtaagcga tcatggcttg
     6541 actctctaca cagaacgtac agatgggaca ggagggttcc ctggaaatct caagatttgg
     6601 atcagttatc acttggaaga aactggtgcc tatgaaatta gctacaaggt aacgaccgat
     6661 caggatacgc tggtcaatcc aaccaaccac agctatttca acttgtctgg tgatttcacg
     6721 cagacgattg accgtcatgt cttccaacta aacacagagg gcatttactc aatcactcct
     6781 gacggtgttc ctgccaaaac tccagaagcc aaccgtgatg tggtcaaaca catctacaat
     6841 ggtaccttgt tgaaggatat ctttgcagaa gaagatgagc aaatccagct ggcatcaggt
     6901 ttggatcacc catttgccct tcctgtaggc catgacaatg ctggattcct ttatgaccaa
     6961 aattcaggtc gcttcctgct tttcaagaca gaagctcctt gctttgtggt ctacacagca
     7021 aactttgtgg atgaaagtgt catcatagga ggtcagccaa tgctacagca caatgggatt
     7081 gctcttgaag cgcaagcttt accagatgcc attcacagtg accttaaagg ccaagtcatt
     7141 cttaaagctg gtcaaacctt caccagtaag acacgttatg aacttgttgt gaaataaaaa
     7201 agctggtcaa aaagtcttta aaattaaaat aacgcatatt gtcaggtgat gaaaagtttg
     7261 atactatgcg ttttattatg aaatgattta ctttatctta tcctgaaatg gtgttatttt
     7321 tgagttatct ggcttgactt tcttgatgga aattatataa tagttgtaat aacaataatt
     7381 acaacgagaa aagaggacca tttatgacct atgaatacaa gagtcacatt tatttggcag
     7441 agacagtttt aaatgtaaag gatttggcca gtcaaacagc cttttatcag caagttattg
     7501 gtttagaaat cttatctcaa actgagacag agtccattct aggccttggt ggaaaagtct
     7561 tggtacagct gattcaagca caagagagcg gagaagtgag ggaacattat ggtctttacc
     7621 atttggccat tctcttgccc acacgcaagg ctttggctga tgtcttgaaa cacctgacag
     7681 atttacagat tcctcttgtc ggcggtgcag accatggtta cagtgaagct atttacttgg
     7741 aagatttgga gggaaatggc attgaactct atcgagataa gccagtttcc acatgggata
     7801 ttcgagaaga tggacgcatt atcggagtga cggaggcttt tgcggcgcag gacatttatg
     7861 agttggggga aagagtagag ccctttatcc tggcagaagg tacgagaatg gggcatattc
     7921 atctttccgt caaggatagt cgaaagtcca gccagtttta tcaaaaggtg ttagggctag
     7981 aggataaatt cagtgtgcct agcgctagtt ggattgcggc tggagattac catcatcatt
     8041 tagcagttaa cgaatgggga ggaaaaggtc tggcttcgcg taagcaaggc ttaccaggtt
     8101 tagcctacta tgtcatcgaa gtcgcacata aagaagaact gttaacgatt gtccaacgag
     8161 cacaagaagt tgacgcacca atcaaatgga tgacatcgag ccaattggaa atcacagact
     8221 cagatggcat cgtgacccgt attcgtttag caaggtaaaa ttaggaattt ctaataagtt
     8281 ataagtattg ctagtttgct ttttgaacgg tctttggtaa cagaactgcg tgagctggat
     8341 gggtgagaat tttgacaaag aagctgatta gagatataat gaagaaaaat cgagcaagga
     8401 aaaagaaatg acaaattcaa agtatattac ttgtttgaaa cgttcagagg gccagttgtg
     8461 tgggattcaa aagatgattg aaggagatcg tgactgtgct gatattgtga cgcaactgac
     8521 agcagtgaga tctagtgtag agcgcgtgat tgagatgata attaccgaaa atcttactga
     8581 atgtatcaat cagccactag atgattctga agctcaaaag gaacgcctag aaaaggctat
     8641 ccgatacttg attaaacgga aataaatgaa tgttttgaga cgatatgtgc aaaggtctat
     8701 taactattag aatagtttaa tgtgagtcaa aagaagttgt aaaaaatctt taaaattaaa
     8761 aaaacgcata gtatcaggtg ttttattatg tagagactta tactcttcga aaatctcttc
     8821 aaaccacatc agcttcacct tgccgtagat atggttactg acttcgtcag ttctatccac
     8881 aacctcaaaa cagtgttttg agctgacttc gtcagttcca tccacaacct taaaacagtg
     8941 ttttgagcag cctgcggcta acttcctagt ttgcacttta attttcattg agtattactt
     9001 catcttctca tgaaattgag tttttgccca gctcctcttg attattctac ttgaccgggc
     9061 caagtattca taccaccttc tacgttggta acggtgaggc cttgggagct gagaaattgg
     9121 aggtagatgc agagcggact cctccttgac aaataacata gtattcatgg tcaggtttga
     9181 gttctttgta gccttgctct aaggtactta atggaagatt tttggcacct ggtgcatgtc
     9241 ctgcttggaa ttcatgtgct tcacggacat cgataagttc tagattttca ttttagtatt
     9301 ttccataaaa gtcagccatg ctgatactag tttccatatt ctactccttc tgggttagcc
     9361 attttgtata gtgaataagc gccgtcaagg ttttggacgg taaatcctgc ttgcttgagg
     9421 ataggctctg cgatatagct gcgcaaacca ctgtggcagc tgacgatgta ggcttgtttt
     9481 ttgtccagtt catcagcttc ctccttgaca ctttgtcagt ttttgataca atagtacaaa
     9541 attagaggag gtgggctatg attcagaaac atgcgattcc cattttagag tttgatgata
     9601 atcctcaggc ggttatcatg cccaatcacg aggggttgga tttgcagttg ccaaagaagt
     9661 gtgtctatgc ttttttaggt gaggagattg accgctatgc gagggaagta ggggcgaact
     9721 gtgttggcga attcgtttct gctaccaaga cctatccagt ctatgtcatc aactacaagg
     9781 gtgaggaggt ctgtctggct caggctcctg ttggctccgc tccagcagcc cagtttatgg
     9841 attggttgat tggctatggt gtggagcaga ttatctctac tgggacctgt ggtgtcctag
     9901 ctgatataga ggaaaatgcc tttctagtcc ctgttcgcgc tttgcgagat gagggagcca
     9961 gttaccacta tgtggcacct tgtcgttata tggaaatgca gccagaggct attgctgcta
    10021 ttgaggaagt tttggaagac agagggattc cttatgaaga agtcatgacc tggacgacag
    10081 acggttttta ccgagaaacg gctgaaaagg tggcttatcg taaggaagaa ggctgtgctg
    10141 ttgtggagat ggagtgttct gctcttgcgg cagtagctca attgcgtggg gttctctggg
    10201 gtgaattgtt gttcacagca gattctctag cggacttgga ccagtacgac agtcgtgact
    10261 ggggctcgga agcttttaat aaggcgctag aactgagttt agcaagtgtt caccaccttt
    10321 agttgtacag gcaaaggatt tgttttatca taaaatgtct agctcatact tttcaaaaat
    10381 atgtttaaac gaggtcacct tcctcttgtc ctaggcatgt tgaggttggg aaaaatcttt
    10441 aaaatcagaa aaacgtatca tatcaggtga tgaaaatttt gatactatgc gttttatgtt
    10501 ggtaagattt agagtgagat gaaatgatac tcttcgaaaa tctcttcaaa ccaggtcagc
    10561 ttcaccttgc cgtatagatg ttactgactt cgtcagttct atctgcaacc tcaaaacagt
    10621 gttttgagct gacttcgtca gttctatcca caacctcaaa acagtgtttt gagcaacctg
    10681 tgactagctt tctaagcgat gccttggttc tcattgccta taatcaaaaa gagaaatttt
    10741 ctcctgaaaa gcatatagag tagctggcgt taaaagctcc tgtcttgctt ttttgaccta
    10801 tagtcacatc tatcaagtat tgttcttgcc taagctatca ataaaaaggt ggcatttttt
    10861 aggcttggtg ttagtgaatt ttgccttatc ctatctaagt catttcgagc tttttatggt
    10921 acaatggaaa catgttattc aaattatcta aggaaaaaat agagctaggc ttatctcgtt
    10981 tatcgccagc ccgtcgtatt tttttgagtt ttgccttggt cattttacta ggctctcttc
    11041 ttttgagctt gccctttgtc caagttgaaa gctcacgagc gacttatttt gatcatcttt
    11101 tcactgctgt ctctgcagtc tgtgtgacag gtctctcaac ccttccagta gctcacacct
    11161 ataatatctg gggccaaata atctgtttgc tcttgattca gatcggtggt ctagggctca
    11221 tgacttttat tggggttttc tatatccaga gcaagcaaaa gcttagtctt cgtagccgtg
    11281 caactattca ggatagtttt agttatggag aaactcgatc tttgagaaag tttgtctatt
    11341 ctatttttct cacgaccttt ttggttgaga gcttgggagc tattttgctt agttttcgcc
    11401 ttattcctca acttggctgg ggacgtggtc tttttagttc catttttcta gcgatctcag
    11461 ccttctgtaa tgccggtttt gataatttag ggagcaccag tttatttgct tttcagaccg
    11521 atttactggt caatctggtg attgcaggct tgattattac aggcggcctt ggttttatgg
    11581 tctggtttga tttggctggt catgtaggaa gaaagaaaaa aggacgtctg cactttcata
    11641 cgaagcttgt actattattg actataggtt tgttgttatt tggaacagca actactctct
    11701 ttcttgagtg gaacaatgct ggaacgattg gcaatctccc tgttgccgat aaggttttag
    11761 ttagcttttt tcaaacagtg acgatgcgaa cagctggctt ttctacgata gattatactc
    11821 aggctcatcc tgtgactctt ttgatttata tcttacagat gtttctaggt ggggcacctg
    11881 gaggaacagc tgggggactc aagattacga cattttttgt cctcttggtc tttgcacgaa
    11941 gtgagcttct aggcttgcct catgccaatg ttgcgagacg aacgatcgcg ccgcgaacgg
    12001 ttcaaaaatc ctttagtgtc tttattatct ttttgatgag cttcttgata ggattgattc
    12061 tgctagggat aacagccaaa ggcaatcctc cctttatcca cctcgtattt gaaaccattt
    12121 cagctcttag tacagttggt gtaacggcaa atctgactcc tgaccttggg aaattggctc
    12181 tcagtgttat catgccactt atgtttatgg gacgaattgg tcccttgacc ttgtttgtta
    12241 gcttggcaga ttaccatcca gaaaagaaag atatgattca ctatatgaaa gcagatatta
    12301 gtattggtta agaaaggaaa gagcatgtca gatcgtacga ttggaatttt gggcttggga
    12361 atttttggga gcagtgtcct agctgcccta gccaagcagg atatgaatat tatcgctatt
    12421 gatgaccacg cagagcgcat caatcagttt gagccagttt tggcgcgtgg agtgattggt
    12481 gacatcacag atgaagaatt attgagatca gcagggattg atacctgcga taccgttgta
    12541 gtcgcgacag gtgaaaatct ggagtcgagt gtgcttgcgg ttatgcactg taagagtttg
    12601 ggggtaccga ctgttattgc taaggtcaaa agtcagaccg ctaagaaagt gctagaaaag
    12661 attggagctg actcggttat ctcgccagag tatgaaatgg ggcagtctct agcacagacc
    12721 attcttttcc ataatagtgt tgatgtcttt cagttggata aaaatgtgtc tatcgtggag
    12781 atgaaaattc ctcagtcttg ggcaggtcaa agtctgagta aattagacct ccgtggcaaa
    12841 tacaatctga atattttggg tttccgagag caggaaaatt ccccattgga tgttgaattt
    12901 ggaccagatg acctcttgaa agcagatacc tatattttgg cagtcatcaa caaccagtat
    12961 ttggataccc tagtagcatt gaattcgtaa agagggatga cccctctttt ttgatgccta
    13021 agatggcaaa tagagacaga agccccttgt cttctagtaa aagttcttca aaggctggac
    13081 tttatggtaa aatagaaaga agtgacaaga gagagtaata ctcaatgaaa atcaaagatc
    13141 aaactaggaa actagctacg ggctgctcaa aacactgttt tgaggttgtg gatagaactg
    13201 acgaagtcag ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagtaaca
    13261 tctatacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt ataagaaaaa
    13321 atcagtcccc taaaggagta gattatgaag ttattgtcta tcgcaatttc tagctataat
    13381 gcagcagcct atcttcatta ctgtgtggag tcgctagtga ttggtggtga gcaagttggg
    13441 attttgatta tcaatgacgg gtctcaggat cagactcagg aaatcgctga gtgtttagct
    13501 agcaagtatc ctaatatcgt tagagccatc tatcaggaaa ataaatgcca tggcggtgcg
    13561 gtcaatcgtg gcttggcaga ggcttctggg cgctatttta aagtagttga cagtgatgac
    13621 tgggtggatc ctcgtgccta cttgaaaatt cttgaaacct tgcaggaact tgagagcaaa
    13681 ggtcaagagg tggatgtctt tgtgaccaat tttgtctatg aaaaggaagg gcagtctcgt
    13741 aagaagagta tgagttacga ttcagtcttg cctgttcggc agatttttgg ctgggaccag
    13801 gtcggaaatt tctccaaagg ccagtatacc atgatgcact cgctgattta tcggacagat
    13861 ttgttgcgtg ctagccagtt ctaactgcct gaacatactt tttatgtcga taatctcttt
    13921 gtctttacgc cccttcagca ggtcaagacc atgtactatc tgcctgtcga tttctatcgt
    13981 tatttgattg ggcgtgagga ccagtctgtc aatgagcaag tgatgattaa gtgcattgac
    14041 cagcaactca aggtcaatcg actcttggta gaccaacttg atttgtccca agtgagtcat
    14101 cccaaaatgc gagaatatct gctgaatcat attgaaatca cgacggtgat ttccagtacc
    14161 ctgctcaacc gatctggaac agcggagcat ctggcaaaaa aacgccaatt gtggacctat
    14221 attcagcaga aaaatccaga agtctttcag gctattcgta agaccatgtt gagccgtttg
    14281 accaaacatt ctgtcttgcc agatcgcaaa ctgtccaatg tcgtctatca aatcaccaaa
    14341 tctgtttatg gatttaatta atataagtat tttataagag ggatttaaga aaaattttaa
    14401 ctttttctta gtccttttta atttcaggag attatactag agtcatcaaa taaagaaaga
    14461 ctctaaggag aatcctatga aattcaatcc aaatcaaaga tatactcgtt ggtctattcg
    14521 ccgtctcagt gtcggtgttg cctcagttgt tgtggctagt ggcttctttg tcctagttgg
    14581 tcagccaagt tctgtacgtg ccgatgtggt caatccgacc ccaggtcaag tcttacctga
    14641 agagacatcg ggaacgaaag agggtgactt atcagaaaaa ccaggagaca ccgttctcac
    14701 tcaagcgaaa cctgagggcg ttactggaaa tacgaattca cttccgacac ctacagaaag
    14761 aactgaagtg agcgaggaaa caaactcttc tagtctggat acactttttg aaaaagatga
    14821 agaagctcaa aaaaatccag agctaacaga tgtcttaaaa gaaactgtag atacagctga
    14881 tgtggatggg acacaagcaa gtccagcaga aactactcct gaacaagtaa aaggtggagt
    14941 gaaagaaaat acaaaagaca gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg
    15001 gaaaggtcct ttcactgccg gtgtaaacca agtaattcct tatgaactat tcgctggtga
    15061 tggtatgtta actcgtctat tactaaaagc ttcggataat gctccttggt ctgacaatgg
    15121 gactgctaaa aatcctgctt tacctcctct tgaaggatta acaaaaggga aatacttcta
    15181 tgaagtagac ttaaatggca atactgttgg taaacaaggt caagctttaa ttgatcaact
    15241 tcgcgctaat ggtactcaaa cttataaagc tactgttaaa gtttacggaa ataaagacgg
    15301 taaagctgac ttgactaatc tagttgctac taaaaatgta gacatcaaca tcaatggatt
    15361 agttgctaaa gaaactgtcg aaaaagctgt taaagataac gttaaagaca gtatcgatgt
    15421 tccagcagcc tacctagaaa aagccaaggg tgaaggtcca ttcacagcag gtgtcaacca
    15481 tgtgattcca tacgaactct tcgcaggtga tggtatgttg actcgtctct tgctcaaggc
    15541 atctgacaag gcaccatggt cagataacgg tgacgctaaa aacccagccc tatctccact
    15601 aggtgaaaac gtgaagacca aaggtcaata cttctatcaa gtagccttgg acggaaatgt
    15661 agctggcaaa gaaaaacaag cgctcattga ccagttccga gc
//
LOCUS       DAVKHZ010000051        15089 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000051 DAVKHZ010000000
VERSION     DAVKHZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.051"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            3..445
                     /locus_tag="VWW99_001462"
                     /pseudo
     CDS             3..445
                     /locus_tag="VWW99_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826702.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            483..785
                     /locus_tag="VWW99_001463"
     CDS             483..785
                     /locus_tag="VWW99_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9539286.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAKQNMV"
     gene            793..1597
                     /locus_tag="VWW99_001464"
     CDS             join(793..1161,1163..1597)
                     /locus_tag="VWW99_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9539287.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDFLMATLQYVREYRTYEEIAADFGIHESNLIRRSRWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKQLANYSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQIPRKSSKLKPLIAEDKAYNHA
                     LSKDRSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            1646..2110
                     /locus_tag="VWW99_001465"
     CDS             1646..2110
                     /locus_tag="VWW99_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9539288.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2268..2693
                     /gene="lacA"
                     /locus_tag="VWW99_001466"
     CDS             2268..2693
                     /gene="lacA"
                     /locus_tag="VWW99_001466"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9539289.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2724..3239
                     /gene="lacB"
                     /locus_tag="VWW99_001467"
     CDS             2724..3239
                     /gene="lacB"
                     /locus_tag="VWW99_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9539290.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3250..4179
                     /locus_tag="VWW99_001468"
     CDS             3250..4179
                     /locus_tag="VWW99_001468"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9539291.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGFLHKESDAELLTKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4181..5161
                     /gene="lacD"
                     /locus_tag="VWW99_001469"
     CDS             4181..5161
                     /gene="lacD"
                     /locus_tag="VWW99_001469"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9539292.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5395..6231
                     /locus_tag="VWW99_001470"
     CDS             5395..6231
                     /locus_tag="VWW99_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9539293.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6267..6584
                     /locus_tag="VWW99_001471"
     CDS             6267..6584
                     /locus_tag="VWW99_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9539294.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6584..8275
                     /locus_tag="VWW99_001472"
     CDS             6584..8275
                     /locus_tag="VWW99_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9539295.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAKYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8490..9896
                     /gene="lacG"
                     /locus_tag="VWW99_001473"
     CDS             8490..9896
                     /gene="lacG"
                     /locus_tag="VWW99_001473"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9539296.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSFNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGKKGSSKYQIKGVGRR
                     VAPDYVPRTNWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9939..10196
                     /locus_tag="VWW99_001474"
     CDS             9939..10196
                     /locus_tag="VWW99_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9539297.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDELEEFKNLFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(10475..11236)
                     /locus_tag="VWW99_001475"
     CDS             complement(10475..11236)
                     /locus_tag="VWW99_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9539298.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(11418..12380)
                     /gene="nrdF"
                     /locus_tag="VWW99_001476"
     CDS             complement(11418..12380)
                     /gene="nrdF"
                     /locus_tag="VWW99_001476"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9539299.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(12568..14727)
                     /gene="nrdE"
                     /locus_tag="VWW99_001477"
     CDS             complement(12568..14727)
                     /gene="nrdE"
                     /locus_tag="VWW99_001477"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9539300.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(14808..15026)
                     /gene="nrdH"
                     /locus_tag="VWW99_001478"
     CDS             complement(14808..15026)
                     /gene="nrdH"
                     /locus_tag="VWW99_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9539301.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         4672 a         2720 c         3091 g         4606 t
ORIGIN      
        1 ttgtgttttg cttagaagcg gataatcaag aataactttt tgatcaggta gcaagcttgt
       61 tggaagaacg agaaattgtc actccaactt atcgtgaagc cttgatcacg cgtgaaaagt
      121 catttccaac tggtttagat atggaatttc taggaaagga cttgcaaatg taatgattcc
      181 tcacacagat attgttcata atctagctga gaaagtggtg gttgttcgat tagagaaacc
      241 ggtgactttc cataatatga ttgcgccagg taaggaagta gaagtatcct tgctcttctt
      301 tatcatcaat aattcaagtt caagtcaaac gaacattctt gctcagttga tggacttttt
      361 cacaggaaat ggacatcttg aagacctatc aaaaatttcc gaaccagaaa aactttatgc
      421 ttatatagat gaagcaatcg cttaatcttg tctattaaaa aataaaatcg gaggaagtct
      481 atatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa
      541 gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat gcagttatgg
      601 tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa
      661 cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattctgta
      721 tcccagcaat gagcgttcca gtatttgaca atattagact tcctgcgaaa caaaatatgg
      781 tatagtagtt ccatgaatta tgaagcaagt aaacaactaa ctgatgcacg atttaagcgt
      841 cttgttggtg ttcaacgcac gacttttgaa gagatattag ctgtattaaa aacagcttat
      901 caacttaaac acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga ctttcttatg
      961 gccactcttc aatatgtgcg agaatatcga acttatgaag aaattgcggc tgattttggt
     1021 atccacgaaa gcaacttaat ccgtcggagc cgatgggttg aagtaactct tgttcaaagt
     1081 ggtgttacga tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg
     1141 gaagtacaaa tcaatcgccc taaaaaaaca attagcgaat tattctggta aaaagaaatt
     1201 tcacgctatg aaggctcaag cgattgtcac aagtcaaggg agaattgttt ctttggatat
     1261 cgctgtgaac tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa atatcggaca
     1321 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc
     1381 acaaattcca cgtaaatcca gcaaactcaa gccgctaata gctgaagata aagcctataa
     1441 ccatgcgcta tctaaggata gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt
     1501 taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc ttcggattac gaatgaattt
     1561 gagtgctggt attatcaatc atgaactagg attctagttt tgcaggaagt ctaataaaga
     1621 aactagcaag gtcaggcctt actttattgg tggacatcca ggatttaatt gtcttctact
     1681 tgatgatgaa gtctatgaag attattatct agagtttgag aaagaagaga tttgctctgt
     1741 tccacgtcct ttcccagaaa caggtatgtt ggatttccaa gatagaagtc catggctaga
     1801 gggacaaaaa gaaatagatc ttagttatga tcttttcagc acagatgcag tgactttgga
     1861 tgaattgcaa tctcgaacaa ttgcccttcg ttctcttaaa catgataagg gattgaaagt
     1921 gcattttgca gaatttccaa acctcatcat ctggtcaact ttgaacaagg gacctttcat
     1981 tacctttgaa ccatggtctg gcttgtcaac attccttgaa gaaggagatc atttagaaga
     2041 taagaagaat gtttgtctct tagaagccaa tcaggttgaa gaattagggt ttgagataga
     2101 agttttataa aaaaagtaaa aaataaataa caaaaacaaa cacaaactgt tgacttcttc
     2161 aaacaatagt agtatattat gtttgtgagg actaattgac tgttttttaa agacagccaa
     2221 tacactgctg gaatccagca tagaaaaaat aaaaaggagt atacaatatg tctattgtta
     2281 tcggtgcaga tgctgcaggt ttgagattga aagaagttgt gaaagacttc ttagaaaaag
     2341 aaaacttcca ccttgtggat gttacagctg aaggtcaaga ctttgttgat gtgactctcg
     2401 ctgttgctgc agaagtaaac aaagaagaac aaaaccttgg tatcgtgatt gatgcttatg
     2461 gagctggtcc atttatggtt gcaactaaga tcaaaggaat ggttgctgca gaagtatctg
     2521 acgaacgttc agcttatatg actcgtggcc acaacaactc acgtatgatc actatgggtg
     2581 cacaacttgt tggtgatgaa ttggctaaaa acatcgctaa aggatttgtt aacggtaaat
     2641 acgatggtgg acgtcatcaa atccgggtcg acatgttgaa caaaatgggc taattagatt
     2701 acagtaagaa aggtaagtta aaaatgagaa ttgcaattgg atgcgaccac atcgtaacag
     2761 atgaaaaaat ggcggtttca gaatttttga aatcaaaagg atatgaagtc attgactttg
     2821 gtacatatga ccatacacgg actcactacc caatctttgg taaaaaagta ggggaagctg
     2881 taactagcgg ccaagctgat cttggggtat gtatctgtgg tactggtgtt ggtatcaaca
     2941 acgctgtaaa taaagttcca ggtgttcgtt ctgccttggt tcgtgatatg acaacagccc
     3001 tttatgctaa agaacaattg aacgctaacg ttatcggctt tggtggtaag attactggtg
     3061 aattgcttat gtgtgatatc atcgaagctt tcattcatgc tgaatacaaa ccatctgaag
     3121 aaaacaagaa attgattgcg aaaattgaac accttgaaag tcacaatgct caacaaacag
     3181 acgcaaactt ctttacagaa ttccttgaaa aatgggatcg cggagaatac cacgactaag
     3241 aggtgaccta tgattttaac agtcacaatg aacccatcca ttgatatttc ctatcccttg
     3301 gatgagttga agattgatac tgtcaatcgt gtggtggatg taaccaaaac ggctggtggt
     3361 aagggactca atgttacccg agtactttca gaatttggcg attctgttct tgctactggt
     3421 ttagtgggtg gcaaacttgg tgagtttttg gttgaacata tcgataatca agtaaagaaa
     3481 gatttcttct caattcaggg agaaactcgt aactgtatcg ctattctcca cggagacaac
     3541 caaacagaag ttcttgaaaa aggtcctgaa gtattggaac aggaaggtca agactttttg
     3601 gaacatttca aaaaactctt ggagtcagtt gaagtagtgg ccatctcagg tagtctgcca
     3661 gctggccttc cagttgatta ctatgcgagc ttggtagaac ttgctaatca agctggcaag
     3721 cctgtagtct tggactgctc aggtgcagca cttcaggctg ttcttgaatc accccataaa
     3781 ccaacagtca tcaaaccaaa taatgaagaa ttgtctcagc ttcttggaag agaagtttct
     3841 gaggatttgg atgaattaaa agaagtactt caagaacctt tgtttgcagg gattgaatgg
     3901 attatcgttt cacttggtgc caacggtact tttgccaaac atggtgacac tttctacaag
     3961 gtagatattc ctagaattca ggtggtaaat cctgttggat ctggagactc tactgtggca
     4021 ggaatttctt caggatttct tcacaaagaa tcggatgcag aattactcac caaggcaaat
     4081 gtccttggta tgctcaatgc tcaagaaaaa atgactggtc atgtcaacat ggccaactat
     4141 caagttctat atgatcaatt aatagtaaaa gaggtataaa atggctttaa cagaacaaaa
     4201 acgtgtacgc ttagaaaaac tttctgatga aaatggtatc atctcagctc ttgcatttga
     4261 ccaacgtggt gctttgaaac gcctcatggc tcaacaccaa acagaagaac caactgtggc
     4321 ccaaatggaa gaacttaaag tcttggtagc agatgaattg actaaatacg cttcatctat
     4381 gcttcttgac cctgagtatg gacttccagc aactaaagct cttgatgaaa aagctggtct
     4441 tctccttgct tatgaaaaaa caggttatga cacaacaagc acaaaacgct tgccagactg
     4501 cttggatgtc tggtctgcaa aacgtattaa agaagaaggt gcagatgcag ttaaattctt
     4561 gctttactat gatgtagata gctcagacga actcaatcaa gaaaaacaag cctacatcga
     4621 acgcatcggt tctgagtgtg tggctgaaga tatcccattc ttccttgaaa ttcttgctta
     4681 cgatgaaaaa attgcggatg caggttctgt agaatacgcg aaagtaaaac cacacaaagt
     4741 tatcggcgct atgaaagtct tttcagaccc acgctttaac attgatgtct tgaaagttga
     4801 agttcctgtt aacattaaat atgttgaagg cttcgctgaa ggtgaagtag tttatacacg
     4861 tgaagaagca gcagccttct tcaaagcgca agatgaagca acgaacttgc catacatcta
     4921 cttgagtgct ggtgtatcag ctaaactctt ccaagatact cttgtatttg ctcatgaatc
     4981 aggtgcaaac tttaacggag ttctttgtgg ccgtgctaca tgggcagggt cagttgaagc
     5041 ttacatcaaa gatggtgaag cagcagctcg cgaatggctt cgcacaactg gatttgaaaa
     5101 cattgatgag ctcaataaag ttcttcaaac aacagcgact tcatggaaag aacgtgtgta
     5161 agaaagtcct cctagtttag gaacatgaat ctaaaaaaat ttaaaaaaag ttgtatgtaa
     5221 aggtttacaa aataacttac ttgtgctata cttaaatcac aagttaatac aaggtgagtg
     5281 ttactaagta atattaggca tgatcacagg tgaattagaa atcagctgat tttctagttc
     5341 atttgtggtc attttttgta cttatatacc tttaagatat aaaaggaggt tgacatgtat
     5401 cgaattctaa atccaatgaa tcacaatgtc tcgcttgtca gaaatgataa gggagaagag
     5461 gtgattgtaa ttggtaaggg gattgcattc ggaaagaaga agggggattt gattgctgaa
     5521 aatcaggttg agaaaatctt tcggatgaag accgaagagt ccagagaaaa ctttatggct
     5581 cttctcaaag atgttccgct tgattttatc acagtgacct atgaaatcat tgataagcta
     5641 tcaaagaaat atcattatcc gattcaagag tatctctatg taaccttgac agatcatatt
     5701 tactgttctt atcaagctct aactcaagga aggtacaagg atagtaatct gccagatatt
     5761 tccgctaagt atcctgtcgc ttttcaaatc gcaaatgaag cttttgaaat ttaccgtcag
     5821 aagctagcag atcattttcc tgaggacgaa attattcgga ttgcttatca tttcattaat
     5881 gctgaaggtg aaaatgaagt ggaacttgtg gagtcgattg ataagaggaa agaaattctc
     5941 aggaatgttg aagaagtttt aacggactat gcaattcaac gaactaaaaa gaataaccat
     6001 ttctatgatc gctttatgat ccatttgaat tatttcttgg attatttaga cagatctaga
     6061 gatgataacc aatcacttct ggatatggaa gatcatatta aacaatccta tccaaaagcc
     6121 ttcgagattg gttccaagat ctatgatgtg attacgcaac atacgggtct tgatttgtat
     6181 aaaagtgaac gagtttatct agttctacat atccaacgtt tattgtcata aaaatttatt
     6241 taaaactata taaggagaat tctatcatga atagagaaga agtaacattg ttaggttttg
     6301 aaatcgtagc ctatgctggc gatgctcgtt caaaactatt ggaagccttg aaggctgctg
     6361 aagctggtga ttttgaaaaa gcggacgctc tggtagagga agctggtagc tgtattgcag
     6421 aggctcacca cgcgcaaaca agtctattga ctaaggaagc ttcaggtgag gacttggctt
     6481 atagtgtaac catgatgcat ggccaagacc acttaatgac aactatcttg ttaaaagatt
     6541 tgatgcatca tttaattgaa ctctacaaga gaggagttaa ataatgaata aactaattgc
     6601 atttatcgag aaaggaaagc ctttctttga aaaactatct cgtaatatct atcttcgtgc
     6661 tattcgtgat ggtttcattg caggtatgcc tgttattctc ttctcaagta tctttatctt
     6721 gattgccttt gtaccaaact catggggctt taaatggtct gatgaagttg tagcctttct
     6781 gatgaaacct tatagctatt ctatgggtat tctggctctc ttggtagctg gtacaacagc
     6841 taagtcattg actgactcag taaaccgtag catggaaaaa accaatcaaa tcaactatat
     6901 gtcaacattg ttggcagcaa ttgttggttt gttgatgttg gcagctgatc ctatcgaaag
     6961 tggtctagct actggattct tggggacaaa aggtttgctt tcagccttcc ttgctgcctt
     7021 cgttactgta gccatctata aggtttgtgt taagaacaac gtcactattc gtatgcctga
     7081 cgaagttcca ccaaatatct cacaagtctt taaagatgtg attccattca ctctctcagt
     7141 tgtttcactt tatgctcttg atttactagc tcgtcatttt gttggtgcaa gcgtagctga
     7201 atcaatcggt aaattctttg caccactctt ctcagcagca gacggatacc ttggtattac
     7261 cattatcttt ggtgcctttg ccttcttctg gtttgtgggt attcatggtc catctattgt
     7321 tgaacctgct atcgcagcaa ttacctatgc aaatgctgaa gtcaacttga accttctcca
     7381 acaaggtatg cacgctgaca agattcttac ttctggtaca caaatgttta tcgttaccat
     7441 gggtggtact ggtgcgacac tggttgttcc attcatgttc atgtggttaa caaaatcaaa
     7501 acgtaaccgt gcaatcggac gtgcttcagt agttcctacc ttcttcggtg taaatgagcc
     7561 aatcttgttt ggtgcacctc ttgttttgaa tccaatcttc ttcattccat ttatctttgc
     7621 tccaattgca aacgtatgga tctttaaatt ctttattgaa actcttggaa tgaactcatt
     7681 cactgctaat ctaccatgga caaccccagc tccactaggt ctagttcttg gtactaactt
     7741 ccaagttcta tcattcattc ttgctgccct tctaatcgtg gttgacgttg tcatttacta
     7801 tccattcctt aaggtctatg atgaacaaat tcttgaagaa gaacgttcag gtaagtctaa
     7861 tgatgaattg aaagaaaaag ttgctgcaaa cttcaacact gcaaaagcgg atgctattct
     7921 tgaaaaagcg ggtgtcgatg cagcacaaaa taccatcact gaagaaacaa atgtcctcgt
     7981 tctctgtgct ggtggaggta caagtggact ccttgcaaat gctttgaata aggcagcagc
     8041 aaaatacaat gtccctgtga aagcagcagc aggtggctat ggtgctcacc gtgaaatgtt
     8101 gccagagttt gatcttgtta tccttgcccc tcaagttgct tcaaactttg aagatatgaa
     8161 ggcagaaaca gataagctcg gtattaaact tgctaagaca gaaggcgctc aatacatcaa
     8221 attaactcgt gatggaaaag gtgccctagc attcgtacaa gagcaattcg attaaggcta
     8281 ggaactgtga aacagtctcc catcgttacg gaaatcgcta tggcgaattt cctaatcgcc
     8341 ttattaattc gtcggtaaaa agatatcgtt tttacctcct catgtcacaa ttcagtgact
     8401 tggcacaaga agtgagatgg agaagcatgg ctcactgact cctctcctct cacttttact
     8461 ttatttaaat caagaaatag gtgaaaaaaa tgacaaaaac acttccaaaa gactttattt
     8521 tcggtggcgc aacagctgct tatcaagcag aaggtgctac acatactgat ggaaaaggac
     8581 cagttgcttg ggataaatat cttgaggata actactggta cactgccgaa ccagctagtg
     8641 atttttacaa tcgatatcca gttgacctca agctagcaga agagtatggt gtcaatggta
     8701 ttcgaatttc tattgcttgg tcacgtattt tcccgactgg ttacggccaa gtaaatgcta
     8761 aaggtgttga gttttatcat aatttatttg cagagtgtca caaacgtcat gttgagccct
     8821 ttgtaactct tcatcacttt gacactccag aagctctcca ctcaaatgga gacttcttaa
     8881 accgtgaaaa tatcgaacat tttgtagact acgctgcctt ctgttttgaa gaatttccag
     8941 aagtaaacta ttggacaacc tttaatgaaa ttggaccaat cggtgatggt caatatttgg
     9001 ttgggaaatt ccctccaggt atccagtacg accttgccaa agtctttcaa tcacaccaca
     9061 atatgatggt gtctcatgca cgcgcggtaa aattgtacaa agagaaaggc tataaagggg
     9121 aaattggtgt tgttcacgcc ctgcctacta aatatcctct agatcctgaa aatccagcag
     9181 atgttcgtgc agctgagttg gaagatatca tccacaataa attcatctta gacgcaactt
     9241 atctaggtcg ctattcagct gaaaccatgg aaggtgtcaa ccatatctta tcattcaatg
     9301 gtggtagttt ggatcttcgt gaagaagatt ttacagcatt agaagctgca aaagacttga
     9361 atgatttcct aggaatcaac tactatatga gtgactggat ggaagccttt gatggagaaa
     9421 ctgaaattat ccataatggt aaaggtaaaa aaggaagctc taagtatcaa atcaaaggtg
     9481 ttggtcgtcg tgtagctcct gactatgtac cacgcacgaa ttgggattgg attatctacc
     9541 ctcaaggttt gtatgaccaa atcatgcgtg tgaagaaaga ttatcctaac tacaagaaga
     9601 tttacatcac tgaaaatggt ctcggctata aagatgagtt cgttgataac actgtttacg
     9661 atgatggtcg tattgattac gtgaaacaac acttggagat cttgtctgat gcgattgcag
     9721 atggagctaa tgtaaaaggt tacttcattt ggtcattaat ggatgtcttc tcatggtcaa
     9781 acggttatga gaaacgttat ggtctcttct acgtagattt tgaaactcaa gaacgttatc
     9841 ctaagaaatc agctcactgg tacaagaaag tagcggaaac tcagattata gactagtaga
     9901 attagtcatt agatatagaa ttttagtgag tcaaaaagat gttcaaagat tttatccaat
     9961 ctatttatga aaaagtttat attataaatt tcgaaaaatg ctctcaaata ccgtgtttga
    10021 cgagtgaaga attgaaaagt cttggaaaat ggtatgtctc gactggtaaa gaatggattt
    10081 gtcattcaga tgatgagctg gaagaattta aaaatctatt tttaaatttt atcaatcctg
    10141 aagaatggga tactatctcc tttgattcag attttatgcc gtttcaacaa tcgtaaccaa
    10201 tttctcaaaa aagttaaatc ttatatttag tactctgtaa aactcttatc taatcacgtt
    10261 acttatactc aatgaaaatc aaagagcaac tttaaactag gaagcgagtc gcagatttct
    10321 caatgtatag ctttgaggaa ttgggcaaaa agtctttgat atagaaaaaa gcatagtatc
    10381 aggtgttgaa acacctgata ctatgctttt tattgtggga agatttactt tttttcttct
    10441 gaaattgagt tgttacccag gctctttcag tttattaagg cttgatgact ttaatgtgtt
    10501 tagatagctt aaaaaggatt gaatcactta gtttagaatc tgaaacaata gtatcaagat
    10561 ttgatacatt ataaaaagta taaaaatcaa acttattgaa cttactatga tctgcgagta
    10621 aatatttttt attagaatta tttaaagcga tgcgttgagc ctctccctct tcctcgctaa
    10681 aagtagctag agctccgttt tgaataccat tacagctaac gaaagcttta gaaaattgga
    10741 gattagagag attttgtagg gtcaatgtac caacaaaagc acctgtaata tcgcgataat
    10801 ttccacctat taaaatcaaa tctgttaatt ttcgttcgct taaaatcaga aaaacaggta
    10861 gactgttggt tacgacgcgg atattgtcaa taggcaactc acgcgcaaaa aactctaatg
    10921 ttgttcctgg tccaatgaaa atagtttctc tttcttctac tagactgcct gcaaaatggg
    10981 ctatttcttg tttttctgcc gtttggaggg cttgtttttc aatatttgat cgctcattag
    11041 tcaaaaggga gttggttcga agtttttcag ctccaccatg cacacgaatc agcaaatctt
    11101 tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc tgaaacggct atttcgtcca
    11161 taatctgttt aactgttatg gttcctttac tatttactat ctctaaaatt ttggttaatt
    11221 tttcttgttt gagcatatta tcacctcgtt tcctactact atcttaccat aaacaaactc
    11281 atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa aaatatcgaa gtttgttttc
    11341 aaaactttcg atatttttgt tgggttataa ctttgatgtt tctagtttac cttttgatga
    11401 ttgagagtga tggagaatta gtctaaaccg tagttatagt catcgtcttg catggcttca
    11461 acttcgccaa gaaggtaacc atttccgact tgagagaaga agtcatggtt ggaagttcct
    11521 gttgaaatac cgttcataac gattgggttg acatcttcag ctgaatctgg gaaaagtgga
    11581 tcttgtccca tgttcatgag agctttattg gcattgtagc gaaggaaggt tttaacctct
    11641 tcagtccaac caacaccgtc ataaagactc tctgtgtagc cttcttcatt ttcataaaga
    11701 gtatagagta ggtcgtacat ccattctttg agtttttctt gctcttcttc aggcaattca
    11761 ttgaaaccaa gttggaattt gtaaccaatg taggttccgt gaacagactc gtcacgaata
    11821 atcaatttaa tgatttctgc aacgttggct agtttgttgt taccgagata gtagagggga
    11881 gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga cgctggcaac tttcttttca
    11941 agtgggctgc cgtttaggta gatttcgttg acaatctcag ccttcttttg taggtaagga
    12001 ttggtattgg tccattcgaa aatttcttca atctcagcct tagtattcaa ggtagaaaag
    12061 attgatgagt aagatttagc gtggacagat tccataaatt ggatgttatt gaaaacagct
    12121 tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa ccccagtttc agattgcata
    12181 gtgtcaagaa gggttaaacc accaaaaact tttccgacca agtctttctc tttgttagat
    12241 agctttctcc agtcatccaa gtcgtttgat aagggaatac gtgtatcgag ccaaaattgc
    12301 tccgtcagtt tttcccaagt tgatttgtcg atgacatctt cgatggcatt ccagttaatg
    12361 gctttgtagt aagtttccat ttaaaatctc tttctgtgtt tagtattgcg aactcacaat
    12421 tatttctact ttaccataat tctataggag tatcgcacaa aaagtcggaa gcccgacttt
    12481 taaaatgtta cataaattat gttatgacat agtagatttg attttatcag tgctgcttag
    12541 ggaaaaataa tgtttctatg ctagaaacta aatcacacag ctttcacatt ggttggcgcc
    12601 gacttctcca ccgtcatctg taaaggtacg gacgtagtag atagacttga ttcccttgtt
    12661 aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt tgtttatttt ccctcttcca
    12721 ttcgtaaagg ccttttggaa tgtcactgcg catgaagagg gtgagtgaaa gtccttgatc
    12781 cacgtgttca gtcgcagcag cgtaaacatc gatgacttta cgcatatcca tatcgtaggc
    12841 agaagtgtag taaggaatgg tttctgtaga caagccagca gcagggtagt agattttacc
    12901 aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc gggtggatag aagcagaaac
    12961 gtcgttgata tagctgatag aaccatttgg cgctacagca aggcgatttt ggtggtaaag
    13021 accatcttct tgaaccttgt cgcgaagttc agcccaatca gcaacaccag ggataaagac
    13081 atttttgaag agttctttaa cacggtctga tgttggaaca aattcaccag ttacatactt
    13141 gtcaaagtaa cttccgttag catagtctga tttttcaaag ttgtggaagg taataccacg
    13201 ttcacgtgca atattgtttg actctaccaa ggtccagtag ttcataagca taaagtagat
    13261 gcttgtaaat tcaacagact caggtgaacc atattcaatg agttgttggg caaggtagct
    13321 gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg ctatttccat ggtcaatcgt
    13381 tggtacagct acgatatgtg aactatctgt aacgaaagta agggcacgaa ccatagcacg
    13441 gatagaacga ccaaaatcag gtgaagtcat catgttaacc acgttggttg aacccaggtt
    13501 acatgaaaca tctgttccca tttgaaggaa ttcttgagca tcgttgatca agcttggttc
    13561 ttgaacttga agaatctcag aacacaagtt actcatgata atctttccat caacaggatt
    13621 tgcacggtta gccgtatcga tgttgactac ataaggatag ccagactctt gttgcaattt
    13681 agagatttca gtttccaaat cccgcgcctt gatttttgtc ttgcgaatat ttggatttgc
    13741 gaccaattca tcgtattttt cagtaatgtc gatgtaattg aatggcacac cgtattcttt
    13801 ttctacagag taagggctga agaggtacat ttcttcattt ttacgagcca attcgtagaa
    13861 tttatcaggt actacaacac caagtgatag agtcttgaca cgtacttttt catcagcgtt
    13921 ttctttctta gttgaaagga aagcgatgat atctgggtga aagacgttga ggtagacaac
    13981 gccggcacct tgacgttgcc ccaattggtt ggagtaagag aagctgtctt caaaaagctt
    14041 cataacagga acgacacctg aagcagctcc ttcatagcct ttgataggtg caccagcttc
    14101 acgaaggttg ctgagggtaa ttcccacacc accaccaata cgtgaaagtt gaagagctga
    14161 gttgatagaa cgtccgatag agttcatatc atccgtcact tggattagga aacaagatac
    14221 caactcccca cgacgagcac gtccagcatt caagaaggaa ggagtagcag gttggtagcg
    14281 ttggtggatg atttcattgg caatatcgat tgcaacagct tcatttccat cagcgaaata
    14341 aagggcgttg aagaagacac ggtcttccat attttcaaga tagtattcac cgtcattagt
    14401 ctttaaggca tattgattgt aaaatttata agctgccatg aatgacttga attggaagtt
    14461 ttggtctttg ataaattgag ccaattcttc caagaactct ggacggtatt tcttgataaa
    14521 ggctgtttcg atgtagttgt gttcaatgag gtaattgatt ttgtctttga ttgaatcaaa
    14581 aaccatagtg tttggaacta cattttcttt aaagaaagca tccaaggctt ccttatcttt
    14641 atgaagcatg atttgtccat taacaggacg gttaatttcg ttattaagac ggaagtaagt
    14701 cacgtcttca agatgtttta atcccataaa atttccctta tctaattaca aaagaaaggc
    14761 ttctaagtta gccctaaaag cagtttcttc tggatgatgt actaagatta tgctaattgt
    14821 ttcagttttc ctggttggaa acctgaaaag acttcagttg gtgtttggat aacaggagct
    14881 gcgctaaaac cgagctcttt aacttgatcg acgtactcag gttgctcatc aagattgatt
    14941 tcacgataag agacattatt actgtccaag aaacgcttgg tcattttaca ttggacacaa
    15001 ttgtttttag aataaacggt taccattgtg taactcctct tcaaaattta atactatctt
    15061 agtatatcag aaaataaaat tttgtcaat
//
LOCUS       DAVKHZ010000052        14591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000052 DAVKHZ010000000
VERSION     DAVKHZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.052"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            473..1594
                     /gene="mnmA"
                     /locus_tag="VWW99_001479"
     CDS             473..1594
                     /gene="mnmA"
                     /locus_tag="VWW99_001479"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9539302.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTCDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1735..2190
                     /locus_tag="VWW99_001480"
     CDS             1735..2190
                     /locus_tag="VWW99_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9539303.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEK"
     gene            2200..4113
                     /gene="mnmG"
                     /locus_tag="VWW99_001481"
     CDS             2200..4113
                     /gene="mnmG"
                     /locus_tag="VWW99_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9539304.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKLEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4320..4412)
                     /locus_tag="VWW99_001482"
                     /pseudo
     CDS             complement(4320..4412)
                     /locus_tag="VWW99_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4466..6145)
                     /locus_tag="VWW99_001483"
     CDS             complement(4466..6145)
                     /locus_tag="VWW99_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9539305.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6147..6380)
                     /locus_tag="VWW99_001484"
     CDS             complement(6147..6380)
                     /locus_tag="VWW99_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9539306.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6660..6875
                     /locus_tag="VWW99_001485"
     CDS             6660..6875
                     /locus_tag="VWW99_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539307.1"
                     /translation="MYNLFAFAIGVRLLAFIVFVACVYGGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VWW99_001486"
     CDS             complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VWW99_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9539308.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VWW99_001487"
     CDS             complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VWW99_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9539309.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7715..8398
                     /gene="tsaB"
                     /locus_tag="VWW99_001488"
     CDS             7715..8398
                     /gene="tsaB"
                     /locus_tag="VWW99_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9539310.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8395..8832
                     /gene="rimI"
                     /locus_tag="VWW99_001489"
     CDS             8395..8832
                     /gene="rimI"
                     /locus_tag="VWW99_001489"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9539311.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIADRKAYYHDPVEDAIIMKREIDEG"
     gene            8822..9832
                     /gene="tsaD"
                     /locus_tag="VWW99_001490"
     CDS             8822..9832
                     /gene="tsaD"
                     /locus_tag="VWW99_001490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9539312.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHELPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEEYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9872..11178)
                     /locus_tag="VWW99_001491"
                     /pseudo
     CDS             complement(9872..11178)
                     /locus_tag="VWW99_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11454..11805)
                     /locus_tag="VWW99_001492"
                     /pseudo
     CDS             complement(11454..11805)
                     /locus_tag="VWW99_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11912..12205)
                     /locus_tag="VWW99_001493"
                     /pseudo
     CDS             complement(11912..12205)
                     /locus_tag="VWW99_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12448..13653
                     /locus_tag="VWW99_001494"
     CDS             12448..13653
                     /locus_tag="VWW99_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9539313.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCIPNN
                     KKIICYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRD
                     NFRLLGEIYPATIVYQISDLLYIPYDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEH
                     RVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYD
                     ELLEKSSHG"
     gene            13646..>14591
                     /locus_tag="VWW99_001495"
     CDS             13646..>14591
                     /locus_tag="VWW99_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9539314.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLFLKYMLLYNFGFFYCIYDNYVQLKF"
BASE COUNT         4301 a         2691 c         3313 g         4286 t
ORIGIN      
        1 ggctcaggtt cccttgcagt caacacaact gtagacgggt atactgtcaa tgaaaacggt
       61 gaatgggttt aaaccgatta aattaaatca tgttaagaac atttgacatt ttaattttga
      121 aacaaagata aggttcgatt gaatagattt atgttcgtat tctttaggta cctgtcttat
      181 gatttcagga aatgtcatta aaaaaacgac tccttttctc taacttgaaa aatagattag
      241 agaaaatggg ttgttttatt tattatagtt atttgaatga agataagaag aaggtatact
      301 cacgtcattc acataatctg tatattgact ataagtttta aaaaacaatt tttaagctct
      361 tccttgtctt ctctaaccaa gcgtgttata atgaatactg ctcaagcgac cttcaatcgt
      421 gaagcacaca cgaccttcaa tcgtgaataa acgaatagat gggagactta ccatgagtga
      481 taactctaaa acacgtgttg tcgtggggat gagtggtggt gttgattcgt cggtgacggc
      541 tcttttgctc aaggagcagg gctacgatgt gatcggtatc ttcatgaaga actgggatga
      601 cacagatgaa aacggcgttt gtacggcgac cgaagattac aaggatgtgg ttgcggtggc
      661 agaccagatt ggcattccct actactctgt caattttgaa aaagagtact gggaccgcgt
      721 ttttgagtat ttcctagcgg aataccgtgc agggcgcacg ccaaatccgg acgttatgtg
      781 caataaggaa atcaagttca aggccttttt ggactatgcc atgaccttgg gggcagacta
      841 tgtagcgact gggcactatg ctcgagtggc gcgtgatgag gatggcactg ttcacatgct
      901 tcgtggcgtg gacaatggca aggatcagac ttatttcctc agccaacttt cgcaagaaca
      961 acttcaaaaa accatgttcc ctctgggaca tttggaaaag cctgaagtac gcagactagc
     1021 agaagaagca ggtctttcga ctgctaagaa gaaagactcg acagggattt gctttatcgg
     1081 agaaaagaac tttaaaaact ttctcagcaa ctacctgcca gctcagcctg gtcgtatgat
     1141 gactgtggat ggtcgcgata tgggcgagca tgctggtctg atgtactata cgattggtca
     1201 gcgtggcggg cttggtatcg gtgggcaaca cggcggtgac aatgcccctt ggttcgttgt
     1261 cggaaaagat ctaagcaaga atattctcta tgtaggccaa ggtttctacc atgattcgct
     1321 catgtcaacc agcctagagg ctagccaagt ccactttact tgtgatatgc ccgaggagtt
     1381 tacgctagaa tgtacggcta aattccgtta ccgtcagcct gactctaagg tgaccgtcca
     1441 tgtcaaagga gacaaggcag aggtcatctt tgcggaacca caacgcgcga ttacaccagg
     1501 acaggcagtt gtcttttacg atggcgaaga gtgtctaggt ggcggtttga ttgacaatgc
     1561 ttaccgcgat ggacaagttt gtcagtacat ttagattgac aaattttctc aatttgctac
     1621 aataataaca gcaatagaaa tgatggtcaa agctcatgga tgttgcaggc ttttttgtcc
     1681 tgcacttctt tgtagttttg actgtttttg tgtcgtttaa gggaaaggac aagaatgact
     1741 caacaagact ttcggacaaa agtagacaat acggtttttg gtgttcgggc gacagctttg
     1801 attgtccaaa atcgcaagct tctagtcacc aaagacaagg gcaagtatta cattatcggc
     1861 ggtgcgattc aagttaacga aaaaacggaa gacgcggtag tccgtgaagt gaaggaagaa
     1921 ctgggtgtta aatctcaagc tgggcagcta gcttttgtgg ttgaaaatcg ttttgaagtg
     1981 gacggcgttt cctatcacaa tatcgagttt cattatctgg tggatttgct tgaagatgcc
     2041 ccgttgacca tgcaggaaga tgagaaaagg cagccctgtg agtggattga cttggataag
     2101 cttgaggata tccagctagt tccagccttt ttaaaaacgg ccctaccaga ttgggaaggc
     2161 caactaagac acatccatct tgagaaatag gagagaaata tgacatatca ttttactgaa
     2221 gaatacgata ttattgtaat tggtgcggga cacgctgggg ttgaggcttc cttggccgct
     2281 agccgtatgg gctgtaaggt cctgcttgcg accatcaata ttgaaatgct ggctttcatg
     2341 ccttgtaatc cctctatcgg tggttctgcc aaggggattg tcgtgcgtga agtcgatgcc
     2401 ctcggtggcg agatggccaa gactattgac aagacttaca tccagatgaa gatgctcaac
     2461 acagggaagg gtcctgctgt tcgtgccctt cgtgcgcagg ctgacaagga actttactct
     2521 aaggagatgc gcaagacagt tgaaaatcaa gaaaatctga cccttcgtca aaccatgatt
     2581 gatgagattt tggtggaaga tggcaaggtt gtcggtgtgc gtacagccac ccatcaagaa
     2641 tatgctgcta aggctgttat tgtgacgaca gggactgctc tccgtgggga aattatcatc
     2701 ggagacctca agtactcatc aggtcctaac cacagcttgg cttctattaa cctagctgac
     2761 aatctcaagg aactgggtct cgaaatcggt cgtttcaaga caggaacccc tccacgtgtc
     2821 aaggcttctt ctatcaatta cgatgtgaca gaaattcagc caggagacga agtgcctaat
     2881 catttctcat acacttcacg tgatgaggat tatgtcaagg accaagtacc atgctggttg
     2941 acctatacca atggtaccag tcatgagatt atccaaaaca acctccaccg tgcgcctatg
     3001 tttacaggtg tggtcaaggg agtggggcct cgttactgtc cgtcgattga agacaagatt
     3061 gtgcgctttg cggacaagga acgtcaccaa ctcttccttg agccagaagg gcgcaatact
     3121 gaggaagtct atgtgcaagg actttcaacc agtctgcctg aggatgtcca gcgtgacttg
     3181 gtgcattcca tcaaaggttt ggaaaatgca gagatgatgc ggacaggtta tgctattgag
     3241 tatgatatgg tcttgcctca tcagttgcgt gcgactttgg aaaccaagaa aatctcaggt
     3301 ctcttcactg ctggtcagac aaatggaacg tcaggttacg aagaggcagc aggccaaggg
     3361 attatcgcgg gtatcaatgc ggctctgaaa atccaaggca agcctgaatt gattctaaaa
     3421 cgaagtgacg gttatatcgg ggtgatgatc gacgacttgg tgaccaaggg aaccattgaa
     3481 ccttaccgtc tcttgaccag tcgtgctgaa taccgtctca ttcttcgtca tgacaatgct
     3541 gatatgcgct tgactgagat gggacgcgag attggccttg tggatgatga acgctgggct
     3601 cgttttgaaa tcaagaaaaa tcaatttgat aatgagatga aacgcctaga cagtatcaaa
     3661 ctcaagccag tcaaggaaac caatgctaag cttgaggaaa tgggcttcaa gccgttgaca
     3721 gatgcggtga cagccaaaga attccttcgc cgtccagaag tttcttacca agatgtggtg
     3781 gccttcatcg gaccagctgc agaagacttg gatgacaaga ttatcgaatt gattgaaaca
     3841 gaaatcaagt atgaaggcta tatttccaaa gccatggacc aggttgccaa gatgaaacgc
     3901 atggaagaaa aacgcattcc ggccaatatc gactgggatg acattgattc tatcgcaacc
     3961 gaagcccgtc agaagttcaa actcatcaat ccagaaacca tcggccaagc cagccgtatt
     4021 tcgggagtaa acccagcaga tatttctatt ttgatggtgt atctggaagg taaaaatcgt
     4081 agtatttcta aaactcttca aaaatcaaaa tgatacgtcg tcggcttctt acgaatgagt
     4141 tcaaatcttg gctttgattc atctccagcc tcccatagtt tcccgaacta tgggagctaa
     4201 ctcaagttat gctttcgctt agccttctag tctgatttcg atttttattg atactcttcg
     4261 aaaatctctt caaaccgcgt caacgtcgcc ttgccgtagg tatggctact gacttcgtca
     4321 gttctatcta caacctcaaa gcagtgcttt gagcaaccta cggctagctt cctagtttgc
     4381 tctttgattt tcattgagta tgagttttaa tctcaaaaga aagcatagag aaaaacagct
     4441 ccgaggacgg ggctgttttc ttgctttatt cttcatctgg tgtgaggatc atcgggatga
     4501 tgatcggttc acgttcggta ttttcataga ggaaggggcg aatagcgttg acaatggcac
     4561 cattgacaga ttgcacgcta gcatccttat ttttcagtgc gatacgaatg gcattgaaga
     4621 ggatacgctg gctttggcga atcaagtcgc cagactctct catgtagaca aagcctcggc
     4681 tgaggatgtc tggaccagat agaatcatct gcgatttgaa gtcaacagtt gcgactgcca
     4741 gaacgacacc gtcttcagat agatcgcgac gatctttgag gacagctgcg ccaatttcac
     4801 cgatacgatt tccatcgaca tagatatctt gggcgttgaa atgacctgcg atacgagctg
     4861 agtcagcagt aagggcaagc acatcgccat tgctcatgat aaagatattg tccttctcaa
     4921 caccagtatc cactgctagt ccagcgtgga ctttttgcat gcggtattca ccgtggacag
     4981 gcatgaagta ttttggctta atcaagcaga gcatgagttt ttgctcttgc tgaccaccgt
     5041 gtccagatgt atggatattg tttactttac cgtggataac ttcgacacca gcttcagaaa
     5101 tgatgttaat cagcttgttg acgctagtag tgtttccagg gatgggacta gaagagaaga
     5161 taacggtatc acctggttgt aattgtactt gacggtgggt tccgttggcg atacgagaga
     5221 gggctgccat aggctcaccc tgactacctg tacagaggat aagaacttct cctgcaggat
     5281 aatctttgat ttcatttggc tcgataaagg ttcccttagg agctttgatg tagccaagat
     5341 cgattccgtt gacaatggcc ttttccatag aacgaccaaa gaccgcaatc ttgcgtccag
     5401 tcttaacagc agcttctgtt gcctgctgga gacggaagat atttgaggca aaggatgcaa
     5461 agatgatacg tccttcaata ccttggataa tcttcataat ggactgacca acgacttttt
     5521 cagagttggt aaaggttggt acttccgcat ttgtcgagtc agacaggaga cagagcacgc
     5581 cttcttcacc aagcgcagcc atacgatgca agtccgcagg ttctccaact ggagtaaagt
     5641 cgaacttaaa gtcacccgta cagacgattt tcccttgagg agtatgaatg acaatcccca
     5701 aaggctctgg aatagagtga gtcgttctaa agaaagttgc cttgagattt ttaaaggtca
     5761 actcggtgtt gtggttgatt tcgtaaagtt tggcgttgcg caagaggccg tgttcttcga
     5821 gtttcccacg gatcaaagcc aaggcaagcg gtccagcgta gatagggaca tttgcttgct
     5881 tgagtaggaa cggaatccca ccaatgtggt cctcgtgtcc gtgtgtgatt aaaacagcct
     5941 tgacgcggtc gatattgtcc acgatgtaag agtagtcagg aatgacatag tcgataccaa
     6001 gcaagtcatc ttctgggaat ttaatcccag catcgacgat gataatctcg tcttggtatt
     6061 caattccgta agtgtttttc ccgatttctc ctagaccacc gatggcaaaa acgccgactt
     6121 cttcaggttt aagagtgtag gccatattag aactccgtaa tttcgaaggc gccagtttct
     6181 ttttcgtaat cgagcaattt gtcagacaag agttcgatat attcgatatt gtactctggg
     6241 cgattttctt cgacaagttg gcgagcagtg atacggccct caagttctga gctggcatcg
     6301 atgtctaggt aaagcgcgcg tgttgtttca cggcgtgggc tacgttcttt tgtttcttga
     6361 taaaaaactt tgtaaatcat atagttcctt tctatttaca ggtcagctta ttccaaactt
     6421 ttagcagaga tttgcttgat ttcattccat tttgtgtcta gattgacctt gattcgttac
     6481 aattatttca attataccac aaaatgaaaa attagtaaaa gacggaaacg agaaagttag
     6541 gggacgattt ctttggtaag cgtttgcaaa aataaagaaa atgtattatg ttgataacgg
     6601 aagtgaaatt tattgcttgt cataattcca tatagatttt aagtcaagga ggttgcccta
     6661 tgtataactt atttgctttt gctatcggag ttcgtttgct agcttttatc gtttttgttg
     6721 cttgcgttta tggaggaaat cttctatttg ccaaactgga acgacgacaa accaagttct
     6781 tgtggaaaat gacctttgtg actctctact cgctttttct cctcctcgtg acctatgtcg
     6841 tttggtttgt attttacttt gaattaaatg cttagatgcc ctgcttatgc tggggttttt
     6901 tgtttgggga tgtagagata gtagatttac gacacgaaaa ttccacccca tctatcaaga
     6961 tgaggtggtc tttgcatttt tctaacatga catgacaatg gaattttcaa agcttattat
     7021 ttttatggga gtcattgttc tgatcaaaaa tagagttgaa gcataaaaat aaaacaatac
     7081 aaacagataa atcgaatggt aaagggatgg tagtaatgtt caaatcattg agaatgctga
     7141 gcaaactgta agagatgaag acaataaaat ttatccttct aaaaatgtgt ttcttatcta
     7201 gcatgactta ccaaactttt tacgaaggtc ttttccaatg gtataacctg ccataaataa
     7261 agcgatacca acaccgacac caaagccacc cttgatttct tgcaattcct tattagaaat
     7321 ttcacgataa ttgttcaaat ctttcatcat attaatctcc tcctaaaaaa taaccagctg
     7381 ccaaaccaga acctataagt gctgttccac ctgcaatagc cttccaagat actgctactc
     7441 caaagataac caagggagcg agaccgccat caatatctga taattcattt tcagataagg
     7501 ataaaaattg attgtcaaga atgtcaaaat ttgtcattga actttcctcc ttttttcttg
     7561 ttcactttta tattcggaaa agaaaaggaa aagtgagcat ttttttaaat tatttttagt
     7621 taagtgaggg cactcttgtc tgggggcaaa gaaaattccc atgtgccagc ttttgtagta
     7681 taatagtaag cagacaaaaa gataggaatg tgttatgaaa gtattagctt ttgatacgtc
     7741 cagcaaggct ctttctctgg ctattttaga ggataagcag gttcttgccg agacgacgat
     7801 taatattaag aaaaatcaca gtattactct tatgcctgcc atcgattttt tgatggcaag
     7861 tttggattgg acacccaagg atttggaccg aatcgtggta gctgaagggc cgggtagcta
     7921 tacaggcttg cgaattgcgg tagcaactgc taagacctta gcttacaccc tgaacatcga
     7981 gttggttggt atgtcgagtc tcttggctct ggtgccccac caacaagaag gtttgtttgt
     8041 ccccttgatg gatgcgcgtc gcaataatgt ttatgcagga ttttatgaaa atgccaaacc
     8101 tgtcatggca gaagcgcacc tatcttttga agaggtgcta gaaaaagtca agggtactag
     8161 tcaggtaacc tttgtcggag aagttggccc ctttgttgag cagattcaaa aacacttgcc
     8221 aaggactgat tacaaagaaa cattgcccaa tgcagccaat cttgctcttt tagcttggga
     8281 caaggaagca gactccttgc atgattttgt gccgaactac ctcaagcgtg tcgaagctga
     8341 ggaaaactgg ctcaagaatc acaccgagtc tggtgagtcc tacattaaac gcctatgata
     8401 gaaatcaagc gaattcaaca acagcctgac ctagctcaag ccatctacgc tgttatggca
     8461 gctgtttacc tagtcagtcc ttggactctg gagcaaatcc aagcagatct gtcccaagac
     8521 cagacttggt atgcattggc ttatgatggg gcagaagtga ttggatttct agctgtgcag
     8581 gagaatcttt ttgaagcaga agtcctgcaa atcgctgtca aaggagctta tcagggtcag
     8641 ggaattgcgt cagccttgtt tgctcaattg ccgacagata aggagatttt cctcgaagtc
     8701 agaaagtcaa atcaacgagc gcaagcattt tacaagaaag aaaaaatggc ggtcatcgct
     8761 gatcgaaagg cctactacca tgacccagtc gaggacgcca ttatcatgaa gagagaaata
     8821 gatgaaggat agatatattt tagcatttga gacatcctgt gatgagacca gtgtcgccgt
     8881 cttgaaaaac gacgatgagc tcttgtccaa tgtcattgct agtcaaattg agagtcacaa
     8941 acgttttggt ggcgtagtgc ccgaagtagc cagtcgtcac catgtcgagg tcattacagc
     9001 ctgtatcgag gaggcattgg cagaagcagg gattaccgaa gaggacgtga cagctgttgc
     9061 ggttacctac ggaccaggct tggtcggagc cttgctagtt ggtttgtcag ctgccaaggc
     9121 ctttgcttgg gctcacgaac ttccactgat tcctgttaat cacatggctg ggcacctcat
     9181 ggcagctcag agtgtggagc ctttggagtt tcccttgcta gccctcttgg tcagcggcgg
     9241 acacacagag ttggtctatg tttctgaggc tggcgattac aagattgttg gggagacacg
     9301 agatgacgcg gttggcgagg cctatgataa ggtcggccgt gtcatgggct tgacctatcc
     9361 tgcaggtcgt gagattgacg agctggctca tcaggggcag gatatttatg atttcccccg
     9421 tgccatgatt aaggaagata atctggagtt ttcattctct ggtttgaagt cagcctttat
     9481 caatcttcat cacaatgccg agcaaaaggg agaaagcctg tctacagaag atttgtgtgc
     9541 ttccttccaa gcagcagtta tggacattct catggcaaaa accaagaagg ctttggagga
     9601 atatcctgtt aaaaccctag ttgtggcagg tggtgtggca gccaataaag gtctcagaga
     9661 acgcttagca gccgaaatca cagatgtcaa ggttatcatc ccccctctgc gactctgcgg
     9721 agacaatgca ggtatgattg cctatgccag cgtcagcgag tggaacaaag aaaacttcgc
     9781 aggctgggac ctcaatgcca aaccaagtct tgcctttgat accatggaat aaagagtggc
     9841 tctttgtcaa gtgtagtggg taggcgaaaa gttacaatct ggagagtacg aaattcgtac
     9901 tctctttttt gatgttcaaa gtcatcaaaa tccgtttttt aaaattgtta aagttccgga
     9961 aaccaaaggc tttgcacttg ataaccttaa tcaaattatt ggtcgcttca agtttagcgt
    10021 tggaataagg cagcttaagt gcgttgataa tcttatctct atcccttaaa aaagtccaaa
    10081 agaccgtttg aaaaagcgga tgaaccgttg gcagttcttg ctcaatcaat ccaaagaaat
    10141 ggtcggcatt cttctcttga aaatgaaaga gcaggagttg atagagttcg tagtgaacct
    10201 gtaatccctc agagtaagaa agcagtttat ctcgtacttc ccgatgggtt aagtgcattc
    10261 gaaacgtggg ctgatagaat cgtttctgac agagcttgaa gctatcctgg tgtagtaaat
    10321 cagtccagtg gactgattta gcccgagcct agaaacgaaa aagcgagggt tccaaaagcg
    10381 cttgagcgcc cgatactcca aggattttcg gtcaaattgg ttcatgattt ggattcgtac
    10441 tcggttcata gctcggctca gatgttggac aatgtggaag cggtcaagaa caatcttcgc
    10501 ctttggaaag agttgcttag cgagccgata ataagggctg tacatgtcca tggtgatgac
    10561 ctcgaccagc tcccgaacct ctctctgata gcgttggaaa tggtttcgaa tcaccgcatg
    10621 agttcgcccg tctaaaattg cgaggatgga tttggactca aaatcttggg caatgaagct
    10681 cattttgctc tttttgaagc tatactcatc ccaactcata acttttggca acttggtcca
    10741 atcggtttca aacttaaatt ccctcagttt tcggatgacg gtggaagttg agaccgccaa
    10801 tctgtgggca atctcagtca tagactgctt ttcaaggagg agctgagcga ttttttgtct
    10861 caccatgttg gaaatctggc aatttttctt gacaatggat gtttgagaaa cgaccacctt
    10921 aaggcaattc ttgcactgaa agcggcgctt tttgagatgc agtaccgtgg gtaaaccctg
    10981 acagtcgaga agcggaattt tagaggcttt ctggaagtcg tatttgatca tcttcccttg
    11041 acaatgagga caaggaggag cgggggaatc caactttgcc tgaacgacta gatgggtttg
    11101 gtatttcaga acagacaaga ctttgatatt tttgtcttcc aatccgagca aatctgtggt
    11161 attcttaagt tgttccatat gacccctttc taatgagttg gttagacgct tttcattata
    11221 ggtcatatgg ggcttttttt ctacaagaaa cgaccctata attcctgggg tgggattacc
    11281 cactacagaa attatagagc caaaacattc caaagtcttg tctgactctg gaatgttttc
    11341 tgttcacagt tgttttgtaa ctgcctaata agtaaccgcg ctataacgag gtatctaccg
    11401 agattgtcaa ttctccagta tactgttata aaaaacaaaa ctggaggatt tttttatgtc
    11461 ccctcatcgt cccatcgact gttccgtagc tgcccaataa cggagtatag tcggctgaaa
    11521 caagaatagg acaaaagtgc ctcaagaaaa gcatcgtaat ttgacaatat tattctgaga
    11581 tgtttttgat gtaagcccat attttctcaa tgggattata ctcgggtgaa taggtaggaa
    11641 gtggtaacag tctatgccct gctccttaca catgttcatt ctgtgaaacc ttgcattgtc
    11701 cataatgata agggatggtg tatctaaagt aggtagtaag aatttttgga agcaagcttc
    11761 gaaaaagtca ctcgtcatcg tatctttata ggtcatcgga gccatagtat attgaaacaa
    11821 aaaagggcat tagtacttct ataacagtat cgcaatctct ggtgtgcgtt gtctcgatct
    11881 taattcaaat aattatatct tcttccagag agactaactg cctgctcaag gctcgatcat
    11941 attcgagata aaaataggtc ttaacccctg tctcataaat ataaatagga gtcaggtagc
    12001 ttaagtgatt caattcttta aggaaccgat ttactttttc tgggtcttgt tcgcagtagg
    12061 tacagctctt ttttagacta tatcccatag cctttagagc gtaatgaata gttgttggag
    12121 gacagttgaa ttcagcagct atttcagtca aataagcatc gggatgagtt tcaatataga
    12181 tctttaattt atctctatca actttttcaa ttaatagtgg aagttttctt gaagttagtg
    12241 tattgaaacg tttttataca gtgctaaaat ttgaagtgta gtgcagatgt atttgtaata
    12301 gtattatagc aattctttgg agaaggtcga gacaagataa aataggtatc tttgagatat
    12361 aagcatgatt tactataaaa ttattgagtt attcataaaa atttctctat caaatatcat
    12421 aatgcgtcgt ttaaggagat ggttacaatg caatatagtt gtggaaaaat aaatattaat
    12481 atccctgatg gatatggaga cattaaggat atagtgtttt cggctcatat tatagttagg
    12541 tataataatg gacattgtgg aggtatagat ccgcatataa ttggactttg taaaaagcag
    12601 atcagaagga tgtctctata tcctattttg ataatcgtat ctagagattc aaaggttatt
    12661 gatgattata aaaatttaga tattgcctat gttgattgta ctcaatgttc aaataatttt
    12721 gaaactgcct tacacgttaa aaatatttta aaattattaa aaatccaact tatacactgt
    12781 catggctact caactaatta ttttttatat atgttaaaaa aacttgacaa gaatggattt
    12841 ggaaaggtta agacagtgat aacatgccat ggatgggttg aatataactt aaaaaagaaa
    12901 ttcttgactt attttgattt ttggacatat tctatgggag atgcttttat ttgtgtatct
    12961 gaaactatga aaaaaagatt ggagagtata ataaaaaata agaagatagt tgcaataaat
    13021 aacggaatta atgtatcaaa tagcgattta gatgtagtag gtgttcaaga ctttaaaaaa
    13081 gaattttgta ttccaaataa caaaaaaatc atttgttatg ttggaaggtt ggatccagaa
    13141 aaaaggccgg atagattcct tgaatttgca gaaaaattat ttttagtcag agaagatgtg
    13201 atttttatta tggctggaaa tggtagtatg tgggctgctt tgaaagaaaa aatttgccat
    13261 ttgaaatgta gagataattt caggttactt ggtgaaatct atccagctac tatagtttac
    13321 caaatatcgg atttattgta tattccttat gatacagaag gaatacctat gtgtgtttta
    13381 gagtcaatgt cacaaggaac tccggtttta gctagtaatg ttggcgggtt aagtgaaatt
    13441 attgaacata gggttgatgg atttttattt gagaaggaag atgttgaggg agtgtgtgct
    13501 tgtgctaatt ttttactcaa tgattctgag tatttgaaat atataggtga gaatagtaaa
    13561 tcaaaaataa gaaaacattt ttctgtgcaa aaaatgtttg tagaaaccat gagagtatat
    13621 gatgaattat tagagaagag tagtcatgga taggaatata gatcaagaat tagtaagtat
    13681 tataatacca acacataata ggtatgaatc tctgattaga gctgtgaaaa gttgtttaca
    13741 tcaaagttat aaaaatatcg aagttataat tattgatgat aattatagta atgtaaattt
    13801 aagaaataaa attatccatc aatttggcta taccaatcat agaattaagt taattttaag
    13861 taatgaagat ttaggtgcaa ctaatgccag aaacataggt ataaaaaatt ctagaggtaa
    13921 gtatatatca tttttagacg atgatgatga atatatgcca gatcgaattt taaagttgat
    13981 ggcttgtttt aaaaagagta gaatgaagaa tttagcttta gtttatagtt atggaataat
    14041 aatttatcca aatggtacac gagaagagga gaagaccgat tttgttggaa atcccttgtt
    14101 tgttcaaatg gttcacaata tagcaggtac gtcattttgg ttgtgtaaaa aagaggtgct
    14161 agaattaatt aatggttttg agaaaataga ttcacatcag gacggtgttg ttttattaaa
    14221 actacttgct caaggatacc aaattgatat agtgcgagaa ttcttggtga attactacgc
    14281 tcacagtaaa gaaaacggta tcactggagt gacacaaaaa acaattaatg cagatgaaga
    14341 atattataat tactgtagga aatattttaa tttattgagt ttcaacgaga gaatattggt
    14401 tacaaagaaa tattattctt taaacataaa gcggttacta ttaataggag acaaatgcaa
    14461 ggctttaaaa gtaatcaaga aggcaagaga agaaaaaatt tttaacgaat ttcttttttt
    14521 gaaatatatg ttattatata attttggttt tttctattgt atatatgaca actatgttca
    14581 attaaaattt a
//
LOCUS       DAVKHZ010000053        14453 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000053 DAVKHZ010000000
VERSION     DAVKHZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.053"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            205..486
                     /locus_tag="VWW99_001496"
     CDS             205..486
                     /locus_tag="VWW99_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9539315.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            618..1562
                     /gene="manA"
                     /locus_tag="VWW99_001497"
     CDS             618..1562
                     /gene="manA"
                     /locus_tag="VWW99_001497"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9539316.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1583..2850)
                     /locus_tag="VWW99_001498"
                     /pseudo
     CDS             complement(1583..2850)
                     /locus_tag="VWW99_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3151..3896
                     /locus_tag="VWW99_001499"
                     /pseudo
     CDS             3151..3896
                     /locus_tag="VWW99_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3925..4380)
                     /locus_tag="VWW99_001500"
     CDS             complement(3925..4380)
                     /locus_tag="VWW99_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9539317.1"
                     /translation="MNRREAVEFVNMCMIKNGDKILVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4400..5575)
                     /locus_tag="VWW99_001501"
     CDS             complement(4400..5575)
                     /locus_tag="VWW99_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9539318.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSLVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5634..6479)
                     /locus_tag="VWW99_001502"
     CDS             complement(5634..6479)
                     /locus_tag="VWW99_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9539319.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6604..7161
                     /locus_tag="VWW99_001503"
     CDS             6604..7161
                     /locus_tag="VWW99_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9539320.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7180..7647
                     /locus_tag="VWW99_001504"
     CDS             7180..7647
                     /locus_tag="VWW99_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9539321.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7736..8365
                     /gene="upp"
                     /locus_tag="VWW99_001505"
     CDS             7736..8365
                     /gene="upp"
                     /locus_tag="VWW99_001505"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9539322.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8539..9129
                     /gene="clpP"
                     /locus_tag="VWW99_001506"
     CDS             8539..9129
                     /gene="clpP"
                     /locus_tag="VWW99_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9539323.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9208..9456
                     /locus_tag="VWW99_001507"
     CDS             9208..9456
                     /locus_tag="VWW99_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9539324.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9557..10717
                     /locus_tag="VWW99_001508"
     CDS             9557..10717
                     /locus_tag="VWW99_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9539325.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLVAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKYFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10985..11854
                     /locus_tag="VWW99_001509"
     CDS             10985..11854
                     /locus_tag="VWW99_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9539326.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLEPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11858..12814
                     /locus_tag="VWW99_001510"
     CDS             11858..12814
                     /locus_tag="VWW99_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9539327.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGFKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12814..13578
                     /locus_tag="VWW99_001511"
     CDS             12814..13578
                     /locus_tag="VWW99_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539328.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKVKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13578..14288
                     /locus_tag="VWW99_001512"
     CDS             13578..14288
                     /locus_tag="VWW99_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539329.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
BASE COUNT         4390 a         2814 c         3113 g         4136 t
ORIGIN      
        1 atttgactgt cctgatcgat ttgccctgtt cttgtttcgt tttactatag aacgatttga
       61 aggcgtttat gatatttagc tgtacgagag tctttaaaaa gtgttttgat ggtttggatt
      121 tcttctttag ttgatttcat atcactatta tataatgctt tttgatttta gtatagtatt
      181 atcgtagaaa atggactcgg tgctatggat acaccggatg agaatggtta tgtagcagat
      241 gactatcgga ttacttactt agaggcccac atcaaggcca tgcgagatgc catttacaaa
      301 gacggggttg acttgcttgg ttatacgaca tggggctgta tcgattcggt ttcagctggc
      361 acaggggaaa tgaataagcg ctatggcttt atctatgtag atcgagataa tgtaggaaat
      421 gggactctca aacgtagcaa gaagaagtcc ttctattggt acatgtcatt tatagcaatg
      481 gtgtaagcat cgagtagagc acatttgttt actattttta taaaatcttc tccctgcttt
      541 ataagatgta aaaaagagtt caattcgaac tctttttttg ctataatgag gggagaaaaa
      601 tcagacagga gatcgacatg tcagaaccat tatttttaca atcagttatg caagaaaaaa
      661 tctggggtgg agccaagcta cgtgatgagt ttggctacga catcccaagt gaaaaaatcg
      721 gagaatattg ggccatctca gcccatccaa atggagtctc taaagttgcc aatggtcgtt
      781 acgagggaac agatcttgct actttgtatg cggaacaccg tgaattattt ggcaatcgtc
      841 cagaacctgt atttccactt ttgaccaaga tcctcgatgc caacgactgg ctcagtgtcc
      901 aagttcaccc agacgatgct tatggactcg agcatgaagg cgaactcgga aaaacagaat
      961 gctggtacat tatcgcagcg gatgaaggtt cagagattat ctacggtcac aatgccaagt
     1021 caaaagaaga actccgccag caaatcgagg acaagaactg ggatgacttg ttgaccaaag
     1081 tccctgttaa ggctggagat ttcttctatg taccaagcgg gactatgcac gctatcgggg
     1141 cgggtatctt aatccttgaa acccagcagt ctagcgatac cacctatcgc gtctatgact
     1201 ttgaccgcaa ggatgacaaa ggcaacttgc gtgaacttca tcttgaaaaa tccatcgatg
     1261 tcttgaacat tggtgagcca gcaaatagcc gtcctgtaac tgttaaagca gatgatttgc
     1321 gttccactct ccttgtatct aatgatttct tcgcagttta caagtgggaa attactggaa
     1381 aagttgactt tgaaaagaca gctgactaca gcttattgag tgtcttagct ggtcaaggtc
     1441 agctaactgt tgacgggaaa aattatccaa ttcaaaaagg gagacacttt atcctaccaa
     1501 gtgatgttga agcttggact ctggaagggc aaggtttgga attgattgtt agccatccat
     1561 aaaaagaaag ggcctgagtt cactactcaa gtcctttttg attacataaa ttgggcaatg
     1621 aagaccacaa tgatgatgat tggaatgacg aaacgaagaa acgaagaaac gaagaaggaa
     1681 gagccagact tggaacagtc cttgtttcca tgctctttca tcgagatgga gttcctccat
     1741 tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa aggtagagag ctccaaatgg
     1801 catgaggaga ttggaagcca agaagtccat agcgtcaaag aagatcttac caaaaatgtg
     1861 aacatccgcc atgacaccgt aagataggga tgaaggaatg ccaaagacaa aggtcaaaat
     1921 tcctaaaata acactccatt tggcacgttt gctgttatcc tggttggtga tattgcccac
     1981 attgatctcc agcatcacga cagaagaagt gacagtcgca aagaggaaaa ggacgtagaa
     2041 aatggttcca aaaggcatct tgtcaaagag ttgaggcaag acgataaaga gcaagctcgg
     2101 tcccccttca atatccagtc tgaaggggga ccgagcttgg aaaatggcta gacctgccat
     2161 gatggatatc gagatattca tggctacgat ggagattcct gactggacta gattggtttt
     2221 cttgtccaag taagaagcat aggtcagcat gactgtaacc cctagtgaga gggcaaagaa
     2281 agattgtccc agagcataga ggggaccagc gctagtcagt tttgaaaagt ctggtttgag
     2341 gaagtaaaga accccttcca tggcatttgg caaactggga gagcgttcga tgatgacgac
     2401 aaagatgata aagagcaggg gcatcatgac tttcgaagct ctttcaatcc ctttttgaac
     2461 cccacgtgat acaataaaga tatttaataa gataaaggct gcttgagctc ctagggcaat
     2521 ggctggattt gaaatgattg aagtaaataa ctgagcataa tcacccgttc caccaagttg
     2581 gaacaatttc ccaaactcaa tacctagata gactagaatc catcctccga taacactgta
     2641 aaaagataag aggataaaga gggcaaaggc gccaatccaa ccgataaagt tgtacttgtt
     2701 attcttgccc agttttccaa aggttttgat agcggaaacg ccagcactac ggccaagggc
     2761 aaactcagcc agcaggagag ggaaatcgat taaaatagtg gaaatgagaa agattagtaa
     2821 aaagcctcca ccgccattag cagcagtcat gtagggaaac ttccaaacgg caccaagccc
     2881 gatggccagc cagcagatgc tagaataaaa ccaagtttcg acccccattg tgatttttct
     2941 gacatagtta aactctgatc tcctttttca aaataagaaa aagctacttg agttgtcaaa
     3001 tagccttctg tttgattgat agacttgact atcctattat gttttctaag gtctgtcaag
     3061 aaaaccattt tcaagttttc acacctttct caaaaacgct tgactctgac ctaaggggag
     3121 gggttatact atcattgtaa ggaggaaatc atgtaccata taaaagaagc tgcgcagctt
     3181 tcaggtgtct ctgtcaagac cctgccctgc atcactatga caagatagga ctcttggtcc
     3241 ccttaaagtc ggaaaacggc tatcgaacct atagtcaaga ggatttggaa cgccttcagg
     3301 tcattcttta ctacaaatat ctaggctttt ctttagagaa aatagcagag ctgttaaagg
     3361 aagaaaggac agatttattg ccccatttaa ctaggcagtt ggactatcta actcgcgaaa
     3421 ggcaacatct ggataccttg atttccacct tgcaaaaaac tattcaagaa caaaaaggag
     3481 aaagaaaaat gaccattgag gaaaaattca cgggatttag ctatcaagac aatcaaaaat
     3541 accaccaaga agcggtagag aaatatggtc aagaagtcat gggacaagcg ctcgaacgcc
     3601 aaaaaggtca cgaagacgag gctacggccg cctttaacca agtctttcaa actttggcac
     3661 aaaatcttca agttggttta cctgcaacag caaccgaaaa ccaagagcaa gcagccaagc
     3721 tcttgcaagc tattcgcact tatggatttg actgctctat tgagatattc ggtcatatcg
     3781 gtaaaggtta cgtctacaac ccagagttta aggaaaacat tgacaagttt ggttctggaa
     3841 cagcccagta cacgtcagat gccattgcgg cttacgttca gacaaatgca gaataaatag
     3901 gctaggaatt tcctagccta ttttttactt caaatcataa agccagtcgt caccgttttt
     3961 gtagtaaaag aattcactga gatcttcttc tagaaacaca cgaagcatat cagacatatc
     4021 atcggttgca agttttagat gagaaagatt ttcaaagtcc tcccaccaaa ctttcccttc
     4081 gtctgaagac tggagttcac cagtaaagtg ttctgtcttg taaaaaagga cgacataacg
     4141 ataatccttg tcgtcatacc agtttttaat accacagagt tggggtttgg aaatgatcag
     4201 accagtttct tctttcactt cacgaatgac agcatcgaca aaggattcgc cacgttcaac
     4261 atgaccacca ggaaaagtaa tgccaggcca gtcgggacta actcggtctt ggaccaggat
     4321 cttatctccg tttttaatca tacacatgtt aacaaattcg actgcctctc ttctgttcat
     4381 tcttcacaac ctttaatctt taatcataat gcagacttcc cgccacccag ccggtacaga
     4441 gagcagaagt gatgttaaag ccacccgtgt gggcattgat atccataact tcgcctgcaa
     4501 agtggaggcc aggtaccagc ttactttcaa gggttttagg attgatttcc ttgagactga
     4561 ctccaccctt ggtaacaaag gactttgcaa gggacatttt tccagttaca ggaattttaa
     4621 gttctttaat ggactggaca agttgttctc gttccttttc agtcagctgt ttgacttttt
     4681 caggatatcc ttgtacaaaa aattcggcca agcgttctgg taacaaggtt tttaaagcgt
     4741 ttttcaagga tttttcccga ttttcttcta gaaatgtaac caagtccttc tcagaaagtt
     4801 gaggcaaaac atcgagtgag agaacctccc cacctttgac caagctagac atgcgtaggg
     4861 cagcaggacc tgacaaacca aagtgggtaa agagtaaatc atgagtgatg acatgcttac
     4921 cataacttag ggtcacatcg tccagagaaa tcccttgtaa ggctttatgt ggaaaatctg
     4981 ttaataaagg actttcagca gcctcaagat cggtgatggt atgcttaaaa tggcgagcaa
     5041 tctcgtgacc aaaaccagtc gaaccagtcg aaggataaga cttaccacct gttgtgacaa
     5101 tgagtttctc acaagtgaag gtttgatccg ctgacttaag gacaaactgg tcatctactt
     5161 ttttaacaga aacgatttct atttgagtag caacttgacc acctagttca gtgattttct
     5221 tttccaaagc ttcgataata gtccgagact tgtcactggc tggaaagacg cgtccgtggt
     5281 cttcgacctt aagtttaaca ccattttctg taaaaaagtt gatgatgtca tgattatcga
     5341 actgggagaa aacactgtaa agaaagcgtc cgtttccagg aattccagct agcaggttgt
     5401 ctaagctacc attgttggtc acattgcaac gtcccccacc agtcccagct aatttttttc
     5461 caagtttccg atttttttcg atgaggaggg ttttctgtcc ataaaagcta ctggaaatcg
     5521 tagccatcat accagcaggt cccccaccga tgacaatagt atcaaaatgt ttcatagctc
     5581 tattgtacca caaaaaaaca agagatgatg gtcacctctt gtcaagaatg caattaatca
     5641 atttcatagc ccatcagcaa accgccctct tctgcataga aactgcagag accagaggtt
     5701 ggtagaattt taatatccgc ttgtgggaag gtttcacgga ttcgctctga gagctgttga
     5761 caacattttt cgttattgcg ttgggccatg acaatacggc caccagcata tccagctttt
     5821 actaactcat cataggcagc ttgaactgat ttctttgatc cccttgcttt ttgtagcaat
     5881 tcgagagtcc cagtttcact agcttttccg accatacgaa tgttgagaag gccaacgacc
     5941 gtaccgataa gcttgctcaa acggccgttc ttcaccaagt tatcgacttt ggctaggaca
     6001 aagagcaact tagttttttc ttgataggcg gtgatagctt caaccacttc ttcaaaagac
     6061 aagccctggt caatcaagtc attcaatttt tctacgagta ggtcaacttc accaccagca
     6121 gataaactat caatcacatg aatcttagtg tcaggatggt cttccagata aatattcttt
     6181 gctagttgag cactattgtg actgccagaa agggtacctg tgatggttac taggaaaatg
     6241 tttttggcac cttcaaatgc tcgcaaatag tcatctgggc ttggacaagc cgattttgaa
     6301 gcttctgcag ttgcatacat ggtttccatc atttggtcaa tatcgagact ggcgtcatca
     6361 acaaagacct gatcagctac ttgaatggtt aaggggacgc ttacaaaggt tgtgttaata
     6421 gctggtgttg gcagttgacg ataatcacaa ccagagtcag caataatttt ccaagtcata
     6481 gaaattctcc atctttgtca gttatacatt gacaaaggtt ctgtcttttt ttacaattat
     6541 atcatgaaaa cccttgaaac aaaagcatca tccgtctgtt gtgacaagta gaaagaaaat
     6601 gttatgtctg aacgtagaat ctctgaaaag tctcttgaaa atctcagaaa atcaaaccaa
     6661 gaatccaatt tattaaccag agaagccatt gaaacagccc tcttgcaact cttggaaaaa
     6721 aaggagctga ccaagattag tatttctgaa ttggtcaaac gtgcgggtgt ttcgcgtgcg
     6781 gccttttatc gcaattatga ctccaaagag gaaattttag agagcgtctt taaacgaact
     6841 gtccacaata ttatggaaca gctgcatcat tacgatttaa agacagacct ttatttggtt
     6901 tgggttcacc ttttccggga ggccagaaag gaagctagag tgattcaatt ggccttggat
     6961 taccatctgg aaaaaatctt tgtccaagcc atgcaggaat ttctagaaaa ataccatggg
     7021 aaatcaaaag gtgtcagctc ttatcttcat tccttctgga gctcagccat cgtctctgtc
     7081 cttctaaaat ggatcaagga tggcatgaag gtaccagctg aaaagattgc agatttaggt
     7141 ttaccatttt ttaaaaaata gagaaaaagg agaaaagaga tgactgaaaa aagactggcc
     7201 tgggatgagt attttgcagc ccaagcccta ctaattgcga atcgttccac ttgtaaacgt
     7261 gctaaagtgg gcgcgattct ggtaaaagat aataaggtta tttccactgg ttacaatggt
     7321 tcggtgtcag ggactgagca ttgtattgat cacgaatgtt tggtcattga aggccactgt
     7381 gttcgcaccc ttcacgctga ggtcaatgct atccttcaag gtgctgagcg tggtgttccc
     7441 aaaggcttta cagcctatgt aacccatttt ccttgtctca actgtacaaa acaattgctt
     7501 caggtcggct gcaagcgtgt ggtttatatc aaccagtacc gaatggacga ctacgcccaa
     7561 tacctttatc aagaaaaagg gacagaattg acccatttac cacttgagac agtacagaca
     7621 gctcttaaag aggctgatct aatgtaaaaa ttatcaaaaa taaatggttt agaaagattt
     7681 ttaaaccgtt ttttggtata ataagaagaa taaattgaaa gaaggaattc caaaaatggg
     7741 aaaaattgaa gttattaatc acccactgat tcaacacaaa ttgtcaatct tgcgtcgtac
     7801 agatacttct acaaaagctt ttcgtgagct agtagatgag attgcaatgt tgatggggta
     7861 tgaagtactt cgtgatcttc cactagaaga tgtggaaatc gaaacaccaa ttacaaaaac
     7921 agttcaaaaa caattggcag gtaagaaatt ggccatcgtc ccaatcttgc gtgcaggtat
     7981 cgggatggtt gatggtctct tgagcttggt tccagctgct aaagttggcc acatcggtat
     8041 gtaccgtgat gaagaaacac ttcaaccagt tgaatacttg gtgaaattgc ctgaggacat
     8101 tgaccaacgt caaattttta tagtagaccc aatgttggca acaggtggct cagcaatctt
     8161 ggctgttgat tctcttaaaa aacgtggcgc atcaaatatc aaatttgtct gccttgtatc
     8221 tgctccagag ggtgtaaaag cccttcaaga agctcatcca gatgtagaaa tctttacagc
     8281 agccttggat gaacgtttga acgaacacgg ttatatcgtt ccaggtcttg gagatgctgg
     8341 agaccgcttg ttcggtacaa aataagatcg aaaagaagaa tgacttggaa aagtatttcc
     8401 agtcacgaaa ggaggttggg tttttgtttc tgtctaatga aagcagagca aaaatttgac
     8461 cttttttgac caagatatta taatagtctt atcttcagtc atttgaccaa caaaattaaa
     8521 actcaaaagg agaaatgaat gattcctgta gttattgaac aaacaagccg tggagaacgt
     8581 tcttacgata tttactcacg tcttctcaaa gaccgcatca ttatgctgac aggtccggtt
     8641 gaagacaata tggctaactc tgttattgcc caattgcttt tcttggatgc ccaagatagt
     8701 acaaaagata tttaccttta tgtcaataca ccaggtggtt ctgtttcagc tggtttggca
     8761 atcgtagata ccatgaactt tatcaaggca gatgtccaaa ccattgttat gggaatggct
     8821 gcatctatgg gaacagtcat cgcatcaagt ggagcaaaag gcaaacgttt catgcttcca
     8881 aatgctgaat acatgattca ccaaccaatg ggcggtacag gtggtggtac ccaacaaact
     8941 gatatggcta tcgctgcaga acacttgctc aaaactcgta ataccttgga aaaaatcttg
     9001 gctgaaaatt caggtcagtc aatggaaaaa gtccatgcag atgcagaacg tgataactgg
     9061 atgagcgccc aggaaacact tgaatatggc tttattgatg aaattatggc caacaattca
     9121 ttgaactaat gatgatagaa ggcaaactcg actgggtttg ctttttttgg tataataggg
     9181 agagatttct tagaaagagg atttatcatg tttgaaaaag tcaatcgctc tggcttgatt
     9241 atctatcttt actataatcg tgatgccaaa aaactgcagg attatggaga tattacctat
     9301 cattccaaga aacatcgtta cttacaactc tatgttccaa ctcaagaagt ggagcaattg
     9361 gtcggacgct tgagcaagga aaaatttatt aaaaaagtta gggtttgtca tatccaagag
     9421 ttggaaacac cctttgtggg caatctttat cgataggaaa acgttatcat cgaaaaagtt
     9481 caaggaaaac attgacaatt ttctgataat tcggtatatt cttaacagac tatttaagaa
     9541 ataaggagac aaaaagatga agaaaaaatt tgccctatcg tttgtggcgc ttgcaagtgt
     9601 agcacttctt gcagcctgtg gagaagtgaa gtctggagca gtcaacactg ctggtaactc
     9661 agtagaggaa aagacaatta aaatcgggtt taactttgaa gaatcaggtt ctttagttgc
     9721 atacggaaca gctgaacaaa aaggtgccca attggctgtt gatgaaatca atgccgcagg
     9781 tggtatcgat ggaaaacaaa tcgaagtagt cgataaagat aataagtctg aaacagctga
     9841 ggctgcttca gttacaacta accttgtaac ccaatctaaa gtatcagcag tcgtaggacc
     9901 tgcaacatct ggtgcgactg cagctgcggt agcgaacgct acaaaagcag gtgttccctt
     9961 gatctcacca agtgcgactc aagatggatt gactaaaggt caagattacc tctttattgg
    10021 aactttccaa gatagcttcc aaggaaaaat tatctcaaac tatgtttctg aaaaattaaa
    10081 tgctaagaaa gttgttcttt acactgacaa tgccagtgac tatgctaaag ggattgccaa
    10141 atatttccgc gagtcataca agggtgaaat cgttgcagat gaaactttcg tagcaggtga
    10201 cacagacttc caagcagccc ttacaaaaat gaaagggaaa gactttgatg ctatcgttgt
    10261 tcctggttac tataatgagg ctggtaagat tgtaaaccaa gcgcgtggta tgggaattga
    10321 caaaccaatc gttggtggtg atggattcaa cggtgaggag tttgtacaac aagcaactgc
    10381 tgaaaaagca tcaaacatct actttatctc aggcttctca actactgtag aagtttcagc
    10441 taaagctaaa gccttccttg acgcttaccg tgctaagtac aatgaagagc cttcaacatt
    10501 tgcagccttg gcttatgatt cagttcacct tgtagcaaac gcagcaaaag gtgctaaaaa
    10561 ttcaggtgaa atcaaggata accttgctaa aacaaaagat tttgaaggtg taactggtca
    10621 aacaagcttc gatgcagacc acaacacagt caaaactgct tacatgatga ccatgaacaa
    10681 tggtaaagtt gaagcagcag aagttgtaaa accataatag aaaaatgttg aaatagggaa
    10741 tgagcctttg actcactccc tgtttcgata tttaatactc ttcgaaaatc tcttcaaact
    10801 gcgtcaacgt cgccttggat tatatatgtg actgacttcg tcagtcttat ctacaacctc
    10861 aaagcagtgc tttgagcaac ctgcggctag tttcctagtt tgctctttga ttttcattga
    10921 gtataagaac ctatcaaaaa gtgagggaaa cccctcggaa ttataaatag aaagagtgaa
    10981 tcttatgctc caacaactcg taaatggttt gattctaggt agtgtttacg cgctgttagc
    11041 cctaggatat accatggttt acggaattat caagctcatc aacttcgccc atggtgatat
    11101 ttatatgatg ggagccttta tcggttattt cttgatcaat tctttccaaa tgaatttctt
    11161 tgtagcgctt attgtagcta tgctagcgac agctattctt ggtgtcgtga ttgagtttct
    11221 tgcttaccga cctttgcgcc actctactcg tattgctgtt ttgattacgg ctattggggt
    11281 ttctttccta ttggagtatg gaatggtcta tctggttggt gccaataccc gtgccttccc
    11341 tcaagcgatt caaacagttc gatatgattt ggaaccaatt agcttaacaa atgtgcagtt
    11401 aatgattttg gctatttcct tgattttgat gattttgtta caagtcattg tccaaaagac
    11461 taagatgggg aaagccatgc gtgcagtatc agtagatagc gacgcggcgc aattgatggg
    11521 gatcaatgta aaccgtacga ttagctttac cttcgctttg ggttctgctc ttgcgggtgc
    11581 ggctggtgtt ctgattgctc tttattataa ctctcttgag cctttgatgg gggttactcc
    11641 aggtcttaaa tctttcgttg ccgcagtact tggtggtatc ggaattattc ctggtgcggc
    11701 tcttggtggc tttgtgattg gtctattgga aacctttgcg actgcctttg ggatgtcaga
    11761 tttccgtgat gccattgttt atggaatctt gttgttgatc ttgattgtcc gcccagctgg
    11821 tatccttggt aagaatgtga aagagaaggt gtaaacgatg aaggaaaatt taaaagttaa
    11881 tattctatgg ttactccttt tgttagctgg ctatagcttg attagtgtac tggtttcagt
    11941 cggagtactt aatctattct atgtacagat tttacagcaa attggaatta atattatttt
    12001 ggctgttggt ctcaacttaa tcgttggttt ttcaggacaa ttttcacttg gtcatgctgg
    12061 tttcatggcg attggtgcct atgcagcagc tattattggt tttaaattac caacctacgg
    12121 tgccttcttt ggagctatgc ttgtaggggc tttgctttca ggagcagttg ccttacttgt
    12181 cggcattcca accttgcgct tgaaggggga ctatcttgcg gtagcaactc tgggtgtttc
    12241 tgaaattatc cgtatcttta tcatcaatgg tggaagcctt acaaatggtg cggcaggtat
    12301 cttagggatt cctaacttta caacttggca aatggtttac ttctttgtcg tgattacaac
    12361 cattgcaacc ttgaacttct tgcgtagccc aattggtcgt tcaaccctct ctgttcgtga
    12421 agatgaaatc gctgctgagt cagttggggt taatacgact aaaattaaaa tcatcgcttt
    12481 tgtctttggt gccattactg caagtattgc tgggtcactt caggcaggat ttatcgggtc
    12541 tgttgtaccg aaagattaca ccttcatcaa ctcaatcaac gttttgatta ttgttgtatt
    12601 tggtggactc ggttccatta caggtgcgat tgtttcggct attgttctgg gaattttgaa
    12661 tatgcttctc caagatgttg ctagtgtgcg tatgattatt tacgctttgg ccttggtatt
    12721 ggtaatgatt ttcagaccag gtggactcct tggaacatgg gaattgagcc tatcacgttt
    12781 ctttaaaaaa tctaagaagg aggaacaaaa ctaatggcat tacttgaagt aaaacagtta
    12841 accaaacatt ttggtggtct aacagctgtt ggagatgtga ctcttgaatt gaacgaaggg
    12901 gaactggttg gattaatcgg tccaaacgga gctgggaaaa ccaccctttt caaccttttg
    12961 accggtgttt atgaaccaag tgagggaaca gtaaccctag atggtcacct tttgaatggg
    13021 aaatcacctt ataagattgc ctctttggga cttggacgta ctttccaaaa tatccgtctt
    13081 tttaaagatt taacagtttt agacaatgtt ttgattgctt ttggaaacca tcacaaacag
    13141 catgttttta ctagtttctt acgcttacca gctttttaca agagtgaaaa agaattaaag
    13201 gttaaagctt tggaattgtt gaaaatcttt gatttagatg gtgatgcaga gactcttgct
    13261 aaaaatcttt cctacggaca acaacgtcgt ttggaaattg ttcgtgccct tgctacggaa
    13321 cctaaaattc tcttcttaga tgaaccagca gcaggtatga acccacagga aacagccgaa
    13381 ttgactgagt taattcgtcg tatcaaagat gagtttaaga ttacaatcat gttgattgaa
    13441 cacgatatga atctggtcat ggaagtaaca gaacgtatct acgtacttga atatggccgt
    13501 ttaatcgctc aaggaactcc agacgaaatt aagaccaata aacgcgttat cgaagcttat
    13561 ctaggaggtg aagcctaatg tctatgttaa aagttgaaaa tctttctgtg cattacggta
    13621 tgatccaagc agttcgtgat gtaagctttg aagttaatga aggagaagtt gtttccctta
    13681 tcggtgccaa cggtgcaggt aagacaacta ttcttcgcac cttgtcaggt ttggttcgac
    13741 caagttcagg aaagattgaa tttttaggtc aagaaatcca aaaaatgcca gctcagaaaa
    13801 tcgtggcaag tggtctttca caagttccag aaggacgcca cgtctttcct ggcttgactg
    13861 ttatggaaaa tcttgaaatg ggagctttct taaagaaaaa tcgtgaagaa aatcaagcta
    13921 acttgaagaa ggttttctca cgctttcctc gtcttgaaga acggaagaac caagatgcag
    13981 ccactctttc agggggggaa caacaaatgc ttgccatggg acgcgccctc atgtcaacac
    14041 caaaacttct tcttttagat gaaccatcaa tgggacttgc cccaatcttt atccaagaaa
    14101 tttttgatat cattcaagat attcagaagc aaggaacaac ggtcctcttg attgaacaaa
    14161 atgccaataa agcacttgca atctctgacc gaggatatgt actggaaaca gggaaaatcg
    14221 tcctatcagg aacaggaaaa gaactcgctt catcagaaga agtcagaaaa gcatatctag
    14281 gtggctaaaa caatccagtg gattgtttta gtcggcagat ggagattacg aagtaatcat
    14341 caatatagtc cgggggacct ttttagtcgg tagattgaga ttgcaaacaa atctgcatct
    14401 acattgaaag cttaatttct aataattgaa aaaatcgaat gaaaaatttc tta
//
LOCUS       DAVKHZ010000054        14409 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000054 DAVKHZ010000000
VERSION     DAVKHZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.054"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            568..1734
                     /locus_tag="VWW99_001513"
     CDS             568..1734
                     /locus_tag="VWW99_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9539330.1"
                     /translation="MKITEVIKKDGSKVYRANVYLGVDQVTGKKVKTKVTGRTQKELK
                     QKANQEKIAFQKAGSTRQKASTIKNYQELANLWLESYKNTVKPNTQGNVRKLIDNHIL
                     PIFGAYKLDKLTTPLIQSIINELADKTNRGEKGAFLHYDKIHALNKRILQYGVTMQAI
                     PSNPARDVVLPRNTQKAKRQKVKHFENQELKKFLGYLDNLDTHRYRYYYETTLYKFLL
                     ATGCRINEALALSWSDIDLDNAVVHVTKTLNRDIEINSPKSKASYRDIDIDQATVSML
                     KQYKLRQTKEAWKIGQRESVVFSDFIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRH
                     THASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL"
     gene            1896..2840
                     /locus_tag="VWW99_001514"
     CDS             1896..2840
                     /locus_tag="VWW99_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9539331.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            2852..3535
                     /locus_tag="VWW99_001515"
     CDS             2852..3535
                     /locus_tag="VWW99_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9539332.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            3790..4821
                     /locus_tag="VWW99_001516"
     CDS             3790..4821
                     /locus_tag="VWW99_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9539333.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCPKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(4976..5653)
                     /locus_tag="VWW99_001517"
     CDS             complement(4976..5653)
                     /locus_tag="VWW99_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539334.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(5650..6213)
                     /locus_tag="VWW99_001518"
     CDS             complement(5650..6213)
                     /locus_tag="VWW99_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9539335.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     VFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            complement(6223..6816)
                     /gene="ruvA"
                     /locus_tag="VWW99_001519"
     CDS             complement(6223..6816)
                     /gene="ruvA"
                     /locus_tag="VWW99_001519"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9539336.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     regulatory      6973..7087
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7221..8321
                     /gene="ribD"
                     /locus_tag="VWW99_001520"
     CDS             7221..8321
                     /gene="ribD"
                     /locus_tag="VWW99_001520"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9539337.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     gene            8306..8941
                     /gene="ribE"
                     /locus_tag="VWW99_001521"
     CDS             8306..8941
                     /gene="ribE"
                     /locus_tag="VWW99_001521"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9539338.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            8961..10166
                     /locus_tag="VWW99_001522"
     CDS             8961..10166
                     /locus_tag="VWW99_001522"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9539339.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRIHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            10167..10634
                     /locus_tag="VWW99_001523"
     CDS             10167..10634
                     /locus_tag="VWW99_001523"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9539340.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(10959..12908)
                     /gene="mutL"
                     /locus_tag="VWW99_001524"
     CDS             complement(10959..12908)
                     /gene="mutL"
                     /locus_tag="VWW99_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9539341.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(13191..13424)
                     /locus_tag="VWW99_001525"
     CDS             complement(13191..13424)
                     /locus_tag="VWW99_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9539342.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            complement(13849..>14409)
                     /locus_tag="VWW99_001526"
     CDS             complement(13849..>14409)
                     /locus_tag="VWW99_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9539343.1"
                     /translation="LNNKLLFKTSISLTLINFFYSFQTVVVPIFSIRYFDGPIFYGIF
                     LTIAGLGGILGNMLAPIVIKYLKSNQIVGVFLFLNGSSWLVAIVIKDYTLSLILFFVC
                     FMSKGVFNIIFNSLYQQIPPHQLLGRVNTTIDSIISFGMPIGSLVAGTLIDLNIELVL
                     IAISIPYFLFSYIFYTDNGLKEFSIY"
BASE COUNT         4730 a         2837 c         2715 g         4127 t
ORIGIN      
        1 ttagttttgt agagaatatt ggagaagtta gaaatgtatg cgctcatgat aatagacttt
       61 ttagctttaa gtgtaagaaa agtgttaaat actattcaga tttacattct aactatggta
      121 ttagtaattc ttgtgcaaaa agtagccctt accatgtttt tatagtctta caatgctttt
      181 taagttcaat tgagtatgct aagttacata atacattact atcaagattt aaaacatttg
      241 aaaaacatct acttacagtc tcaattgata agtttttaac tgaattagga tttcctgcaa
      301 actggcatag aacaatgccc aagttaccac aatagttagt aaaactatac aatttaatga
      361 aattatggaa cttaactatt aattttatga acaggaattt aaaactttag ataatactag
      421 ccatatccgc cttgttccct cctctggtat tatttaccgt ctgactactt aaaatcgaat
      481 acagggcaaa ctatgaagcc ctagcatgat ataaaacctt tttgataatg gcttgcctgc
      541 tgatgtattt tagaaaggtt tatcatcatg aaaataactg aagttataaa aaaagacggt
      601 agtaaagtct atcgtgctaa tgtatatcta ggagttgacc aggtaacagg aaagaaagtc
      661 aaaaccaagg taacaggtcg gacacaaaag gaattaaaac agaaagccaa tcaagaaaaa
      721 atagcttttc aaaaagcagg atctactaga caaaaggcta gcaccataaa aaactatcaa
      781 gaattagcca atctctggtt agaaagttat aagaatacag ttaagccaaa cactcaagga
      841 aatgtcagaa agctaatcga taatcatata ttgcctattt ttggggctta caagctcgat
      901 aagctcacta ctccacttat ccagtccatt atcaacgaac ttgctgataa gactaataga
      961 ggggaaaaag gggcttttct acattatgac aagatacacg ctttaaacaa acgtatctta
     1021 cagtatggcg taaccatgca agctataccg tccaatcctg cgcgtgatgt tgttttacct
     1081 cgtaacactc agaaagcaaa gcgccaaaag gtaaaacact ttgaaaatca agaactaaaa
     1141 aagtttcttg gttatctcga taatttagat actcatagat accgttatta ctatgagaca
     1201 acgctctata agttcctatt ggctactggt tgccgtatca atgaagcttt agctctttcc
     1261 tggtctgata tagatttaga taatgccgtg gtacatgtta ccaaaacact aaatcgggat
     1321 atagaaatca atagcccaaa atctaaggct agttatcggg atatagatat agatcaagcg
     1381 actgttagca tgctaaagca gtacaaactg cgccaaacta aagaggcctg gaagataggg
     1441 caacgtgaaa gtgtagtatt ttctgatttc attcatgagt atcctagcag ttcaagactt
     1501 aaaagaagat tgcaaacaca ttttaagcgt gctgatgttc ctaacattgg ctttcacgga
     1561 ttccgtcata ctcacgctag tttattgctt aactctggaa taccatacaa ggaactccag
     1621 taccgcctag gtcattccac tttatcaatg accatggata tatatagcca cctctcaaaa
     1681 gagaacgcaa aaaaagcagt atcattctac gaaactgcac taaaagcact atagggggga
     1741 gcaaaaaggg gagcaaattt aaaaatcaac atttcaagac aaagtaaaaa tccaatagca
     1801 acaggatttt tgttagattt attttgaaaa aggccgattt tgtagtataa tagtacagtg
     1861 tgaaaaaatc tgaaaaatga gaaaggataa gggatatgaa acaagttttt ctctctacaa
     1921 caactgaatt taaagagatc gatacgcttg aaccgggtac ttggatcaat ctcgtcaatc
     1981 cgactcaaaa tgaatcactc gaaatcgcca acaccttcga tattgatatt gccgaccttc
     2041 gagcaccgct cgatgcggaa gaaatgtctc gtattaccat tgaagacgag tataccctga
     2101 ttatcgtaga cgtgccggtc acggaggaaa gaaataaccg cacctactac gtaaccatcc
     2161 cgcttggtat tatcatcact gaggaaacca ttatcactac gtgtttggaa ccactacctg
     2221 tccttgatgt ctttatcaac cgtcgattgc gtaatttcta taccttcatg cgttcacgtt
     2281 ttatctttca aattctttat cgcaatgcag agctttacct aacagccctt cgttcaatcg
     2341 accgcaagag tgaacaaatc gaaagtcaac tgcatcaatc aactcgtaat gaagaattga
     2401 ttgagctcat ggaattggaa aaaactatcg tctatttcaa ggcctccctc aaaacaaatg
     2461 agcgcgtgat taagaaattg accagttcaa ccagcaatat caagaaatac cttgaggacg
     2521 aagacctgct tgaagacacc ctgattgaaa cccaacaggc catcgagatg gcagatattt
     2581 atggaaacgt cttgcattct atgacagaga cctttgcctc tatcatttct aacaaccaga
     2641 acaacatcat gaaaaccttg gcccttgtga ccatcgtcat gtccatccca accatggtct
     2701 tttctgccta cgggatgaac tttaaggata atgaaatccc cctaaacgga gagccaaatg
     2761 ccttctggtt aatcgtcttt atcgcctttg ctatgagtgt ctcgctcact ctctatctca
     2821 tccataaaaa atggttctaa gaggagttcc tatgtctcaa attgatctac aaaaattaac
     2881 taagaaaaac caagagtttg tccacattgc tacccaacaa ttcatcaaag atgggaaaac
     2941 agacgctgaa atccagacta tttttgagga agtcattccc caaatccttg aggagcaatc
     3001 taaaggtaca actgcccgtt ccctatacgg cgcaccaact cattgggctc atagcttcac
     3061 tgtcaaagag cagtacgaaa aagagcatcc aaaagaaaat gatgacccaa aactgatgat
     3121 tatggactca gctcttttca tcactagcct ctttgccctt gtcagcgccc tcacaacctt
     3181 ctttgcggca gaccaagctt tcggctatgg attgattact cttctattag ttggactggt
     3241 tggtggattt gccttctact tgatgtacta ctttgtttac caatactatg gaccagatat
     3301 ggatcgcagt caacgtccac ctttctggaa atctgtacta gttatcctag cttctatgtt
     3361 cctttggttg cttgtcttct ttgcaacaag cttcctacca gctagcctta acccagtact
     3421 ggatccattg ccactagcta ttattggagc agccctccta gcccttcgct tctatctcaa
     3481 gaaacgcttg aatatccgta gtgcaagtgc aggaccaaca cgctatcaag aataagaaaa
     3541 cgataaaagc aactgcaggt gcggttgctt tttcacttac ttttttgagt tatattcaat
     3601 gaaaatcaaa gagcaaacta ggaagctagc tgcaggttgc tcaaagcaca gctttgaggt
     3661 tgcagataaa actgacgtgg tttgaagaga ttttcgaaga gtattaaaag tattcttctg
     3721 aaatcccaca tagctttctc ttatattttg tgataaaata ggctcaatct atttctagga
     3781 ggatgagata tggtttctac tattggtatt gttagtttat ctagtggcat tatcggagag
     3841 gattttgtca aacacgaagt ggacttgggt atccaacgtc tcaaggatct gggactcaat
     3901 cccatctttt tgccccattc gttaaaagga ttagacttta tcaaggacca tcctgaagct
     3961 cgtgcagagg atttgattca tgccttttct gatgatagca tcgacatgat cctatgtgcc
     4021 atcggtggag acgataccta tcgcttgcta ccttatcttt ttgaaaatga ccaactccaa
     4081 aaggttatca agcaaaaaat ttttcttggc ttctcggata caaccatgaa ccacctcatg
     4141 ttgcataaat taggaatcaa gactttttat ggtcaatcct ttttagcaga catttgtgaa
     4201 ttagacaaag aaatgctagc ctatagcctt cactacttta aagaattgat tgagacggga
     4261 agaatttcag aaatccgccc tagtgacgtt tggtatgagg aacgaactga ttttagtccc
     4321 acggccctgg gaacacctcg tgtcagtcat acaaatacag gttttgactt gttgcaagga
     4381 agtgctcagt tcgaggggaa aatcctcggt ggttgcctag aatccctcta cgatatcttt
     4441 gacaactctc gatacgcaga tagcacggaa ctctgcccaa aatacaaact tttccctgac
     4501 ttgtcagact gggaagggaa aatcctcttg ctcgaaacaa gcgaagaaaa gcctaagcca
     4561 gaagacttca aaaagatgtt gctaacttta aaggacactg gcatattcgc ggtcatcaat
     4621 ggactcttgg tcggaaaacc tatggatgaa actttccatg acgactataa agaggcacta
     4681 ttagatatca ttgacagcaa tatcccgatt gtctataatc tgaatgtcgg ccacgcaact
     4741 ccaagagcaa ttgtaccctt tggggtccat gctcatgtag atgcacagga acaagtcatt
     4801 cgctttgact ataacaaata aagctgggaa aagtttctca gcttttttct cggtatcatt
     4861 actggcattt tcatcacatg ttacagtgta ttgaaactag aatagtacac ctctacttct
     4921 aaaacattgt tagaaatcga tttgactgtc ctgattgatt tgtcctgttc ttatttcatt
     4981 ttactatact gatagctaaa atgattaagg agaggctgtt gcttaacatg tgaacaagta
     5041 ggggatagta gatattctct ttttctttca cataaataat agaaaaggca aggcctccac
     5101 ctaagtaacc aactgcacca atccactctg atagagccaa actgtgcata tgagtcaaag
     5161 caaaaacaag tcctaccaga ataatgctta gtaaacctgg caaccgttcc tgcaagtaat
     5221 gcaataagac ctgacggaaa aataattctt ctaccagagg tccaatgaca caagcaaaaa
     5281 cagctatcag tagtggttgt tcttgaaagg tactttgaat attagactga tttagacctt
     5341 gtccatctag tctcacaaac tgcttcaaca tctctgatac aaattcaaag acaacagtca
     5401 tcagaatgag aaagagccat cctgttaaga cgccaaagaa gaattttctt ttagtctttc
     5461 taatctcctt ccattgttgg atcaatctat ccttgaacaa gaaggagcca tataaagcta
     5521 aaactacata aacaagataa tttgcaatga cagatggcat taaagaagcc agcaatttaa
     5581 cgccatcaaa cacaaagagg actgtataga gaatcgcaaa aatcagaatt cccttatttt
     5641 tgttagacat catttaagac ctttccactc acaatcattc tcgtggtcat caactagccc
     5701 tgcagcctgt agaaaagaca atacggcgac tgggcctgtg aacttgaagc ctcgtttttt
     5761 gagatctttg gctaatttct cagataaggg tgttttagct ggcgcttggc gataatcagg
     5821 aacatcgtta acgacagttt tcccctcaac aaaagaccaa agataggcat caaaagagcc
     5881 gtactctgcc tgtaactgta gaaagacttg ggcgttagcg cgtgtagcaa aaagcttggc
     5941 tctatttcga atgatagctg gattctccag catggcttcc aattcagtgt cagtcatctc
     6001 tgcgactgag tgaatttgat agctatgaaa gacttctcgg aaagcttggc gtttgttgag
     6061 taccgtttcc caagacaggc ctgcctgata ggtttccata cacaacaact caaacaatac
     6121 ttggtcatca tggaggggct ggccccactc ctcatcatga taggcgatgt agagcgggtt
     6181 ggtcatcttg acccacgaac aacgttttgt cattctctgc tcctatttga ccaacatttt
     6241 aagggccgac ttgatatagt tctcagctgt atctgtcgtt ccttcaaaga atttcttgat
     6301 tttcttgagc tctgttgcct tgtagcccag agccaacatg gcttccatag cttcttccaa
     6361 ttcttggttt tcagcacttg cttgcactgc gaccttggca ggaaggtcat ctcctgcaac
     6421 tactaccttg ccttccaagt ccagcaccat ctgctgggct gttttcttgc caattttagg
     6481 gaacttggtc aagtaggtga tgttcttggt ttcaatggct tgaaccaagc cagcattgtc
     6541 atcagcagcg ataatagcaa gagctgatac aggaccaatc ccagagaccg aaatcagact
     6601 aagaaagagc tttttctcat cctctgagcg aaatccataa agcaaatggg cgtcctcacg
     6661 cacaacctga tgcacataaa tctgagcctc ctgattaacc tgacctgaat aggcataagg
     6721 attggccaca tgcaggatat aaccaatacc attggtttca agaacaatgt atttggcagt
     6781 aattttggta atgattcctt ttaaatatgc gtacatagta ttccttctct cttatttcgt
     6841 ttatctagta tatcatattt tctttatccg aggagcgaga atcgattatc acaaatacag
     6901 tatatcaaaa acaatcggta tttatctgtt gacatattca aaattaagta ctataatcat
     6961 ctgtaattga ataactatct tcagggcagg gtgaaattcc ctaccggtgg tatagcccac
     7021 gagccgaaag gcatgatttg gtgaaattcc aaagccgaca gtatagtctg gatgaaagaa
     7081 gataaaagta tatttgtgct ttttgcgtgc tctgaaatga ttacttgtca tttcagagca
     7141 tttttgttaa tcgcataagt tttcttttgt atgccttgag tagtccccta ttcaaggcat
     7201 atttttttgg aggtagtgag atgagcgatt caaaatatat gaaattagca ataaaactgg
     7261 cacaaaaagg ggctggttac gtcaatccca atcctatggt cggcgcagtt attgtaaaag
     7321 ataatcacat tatcggacaa ggttatcatg agttttttgg tggcccacat gctgagagaa
     7381 atgctcttaa aaactgtaga gaatcccctg tcggagcgac gctttatgta acacttgaac
     7441 cctgttgtca cttcgggaaa acacctccct gtatagatgc tataatcgat agtggtatta
     7501 caagagtagt cattggaagc ctagactgta atcctattgt atctggaaaa ggagtaaaga
     7561 tacttgagga aaataatctt caagttactg ttggaatttt agaaaatgag tgtcttaact
     7621 taataaaaag ttttagaaag tatattaccc agcatgtacc ctatgttttt atgaaatatg
     7681 caatgtcaat ggatggaaaa atagccacta aaacaaatca atccaaatgg attactgaag
     7741 aagaagcaag aaagcatgtg catcagttac gacaccatgt tagtgcaatt atggtgggag
     7801 tcaatactgt tattcaagac gatcctttgc tgacatgtag attggaggaa gggaaaaatc
     7861 ctatccgtat catatgcgat acacatttac gaactcctct tacctctaaa atcgtaaaaa
     7921 cagcaaatga tattaaaact tacattgcca cttcctctga agacaaaaat aaaatgaagc
     7981 tatatcaaaa tcatggctgt gaaatacttt ccataaagaa aaaaggcaat catatagact
     8041 tatcgagttt aatgcaacat ctaggaaaca tgcagattga tagcctagtt ctagaagggg
     8101 gcagtctaat gaattggagt gctttggaac aacaaattgt tgatgagctg aaaatatata
     8161 ttgcaccaaa aatttttgga ggtagtgcca agtttcctgt cggaggtgaa ggtatttctt
     8221 tgccaaatga cgctattaga ttgaaacctt atgcattttc tcaaatagga aatgactatc
     8281 tcatagaaag tgaagtgatt tatccatgtt cacaggaata attgaagaaa tcggaaaagt
     8341 tgaaagaata cagaaagact ctcgtaattg taaactatca attaaagcct caaaaatatt
     8401 aacggatatc catttaggcg atagtatagc agtaaatggt atctgtctta cagttactca
     8461 tttcaatcat caatccttta cagttgatgt aatgaatgaa acatggagtc gaacagctct
     8521 tactctatta aaacatggaa gtgaggtgaa tctagaaaga gccttatctg tcaacggtcg
     8581 acttgggggt cacgtcgtta caggacacat tgatggtaca ggaaaaatct cgtcaataaa
     8641 aaaagatgat aatgctgtat ggtatcaaat caacacacaa aaagaaattt tagatttaat
     8701 agttgaaaaa ggatctatta caattgacgg cattagtctg actgtcgcta aagtctccaa
     8761 agtaaacttt tcagtatctg ttatccctca taccttggaa caaaccattc ttaagagtaa
     8821 acaagtcggg agtacagtaa atcttgaaaa tgatatctta ggtaaatatg tgcaaaaact
     8881 gatggataac tctccaaaat cagaaatatc taaggaacta ttatatcaaa atggatttta
     8941 gcagaaagga taatcagtca atggaatatc gaaaaataca agaagcatta gaagcattgc
     9001 agaagggacg acttgttctt gttatagacg acaaggatag agaaaatgaa ggagacttaa
     9061 tttgttctgc acaagcagct acaacagaaa atgttaattt tatggctact tatgccaaag
     9121 gattaatttg tatgcctatg agcgaaagtt tagctaatca attaatgctt tcacctatgg
     9181 ttgaaaacaa tacagataat cataagactg cttttacagt ttcaattgat tataaagaaa
     9241 cgaccacagg tatttctgcc gaggaaagag gactgaccgc acgtatgtgt gtagctgaag
     9301 atataacacc ctctgatttt cgcaggccag gacacatgtt tcctttaatt gcaaaaaaag
     9361 gtggtgttct agaaagaagt ggacacacag aagcaactgt tgatttatta aaattagctg
     9421 gactaaaaga gtgtggccta tgttgtgaaa taatgaatca tgatggcaaa atgatgagaa
     9481 cagatgattt aattcagttc tcgaagaaac acaacattcc actaattacc atcaaagaat
     9541 tacaagaata tagaaaagta tatgatcagc tggtagaacg agtttcaact gtcaatatgc
     9601 ctactagata cggtaatttc aaagcaatta gctatataga taaactaaat ggggaacatc
     9661 atcttgctct tattatggga aacatagagg atgaagccaa tgtattatgt cggatccact
     9721 ccgaatgttt aacaggggat gttttaggct ctttacgttg cgattgtgga cagcaattcg
     9781 ataaagctat gaaaatgatt gttgagaatg gttcgggtgt cttactttac ttgcgacagg
     9841 agggacgagg aattggactt atcaataaat taaaagccta tcatttacaa gatcaaggca
     9901 tggatacgct tgatgccaat cttgcattag gctttgaagg tgatttaaga gaatatcata
     9961 ttggagcaca aatgcttaaa gatctgggac ttcagtcact tcatttactg acaaataatc
    10021 ctgacaaggt tgaacagtta gaaaaatatg gaattactat ttccagtaga atatcaatcg
    10081 aaatagaagc caatccttac gatagttttt atttagaaac aaagaaaaat cgaatgggtc
    10141 acattttaaa tatggaggaa aaataaatga acacttatga aggtaattta gtagcaaaca
    10201 atattaaaat aggtattgtt gtagcgagat ttaatgaatt tataacttca aaattattat
    10261 ctggagcact agataatctc aaaagagaga atgtaaacga gaaagatata gaggtagcct
    10321 gggttccagg agcttttgaa atatcactga ttgcatcaaa aatggcaaaa agtaaaaaat
    10381 atgatgcaat tatctgcttg ggagctgtca ttagagggaa tacaagtcat tatgattatg
    10441 tatgtagcga ggtatctaag ggaatcgccc aaatcagttt aaatagcgaa attcctgtta
    10501 tgtttggtgt gctaacgaca gatacaattg aacaagccat agaacgagct ggtactaaag
    10561 caggaaataa gggttctgag tgtgcacaag gagctattga aatggtcaac ctaattcgta
    10621 cattagacgc atagaacaga ctctactttt agacgaataa agaaacttgg caaaatcgca
    10681 atttaatact ctgtgaaaat catagagcaa actaggaaac cagtcgcaga gtggtcaaac
    10741 acaacttcga tgttacagac aaaactggca aaattagctc aaaacactgt tttgagatta
    10801 ctgataaaac tgacgtcgtt taaaaagagt ttcgaaaaga attaggaaaa aatgaagtaa
    10861 atatttctat gataaaacgc ataaaatcaa ggtttttgaa cacctgattt tatgcgtttt
    10921 tctgattttg aaaatttttc ccagactttt ttatactttt aatatttccc caactcacgg
    10981 agactggtgt gattttcctg aatacgtcgg aacatctttt ccatatccga cttggtaaaa
    11041 tgcaccaaaa caggacgtcc gtgaggacag ttatagggat tgtcacattg agaaagctga
    11101 tagaggagtt gtctagctga atgatcatca atacgatgat tggccttgat agatcgcttg
    11161 caagacatca tgatagccag ctctgctcgg tatttcttga tagaaacttc cttggtcaaa
    11221 aggagcatgt cgcacatctc atagatgcct gattcaatct cttcttctgc catccaaata
    11281 ggatgttcac gtagaataaa ttgattttct ccgtactctg ctagaaagac gcccacttcc
    11341 tctaagagag gcattctttc cttgagacgc agggcatcat ccgcaggaaa ttcaaagata
    11401 tagggcacta ggagttgctg ctggctctgg tcaacattgc caatgctttc acggtactcc
    11461 tcgtacttga cccgttcctg agcagcgtgc tgatctatga tgtaaagtcc atctcgccct
    11521 tgggcaaaga gataagtccc gtgcatttgt ccgaaaaact ccaactctgg gaagctggat
    11581 gcttcttctc gctccagttt gtcataagcc ttatcgatgc tagcaagatc taactctgga
    11641 tggtctagct ggtcgtagtt agcaggcttt ctctctgcaa aatgcagttt tgctggcttg
    11701 gtcaatcggt ccaaggtttc cttggcaaac agggtcaaat cctgcccttc atcagtcaat
    11761 tctacctgat aatcagctac ttcagtttga ctaggtcttg acggctcagt tttctcatag
    11821 tagagcgtat tttctttgag tgggagaata gtttgctcca ccttctcacg attgcgcacg
    11881 gtcgatttgg caagattttc caaggcatct ggaatcaagg tttgttcctt gagactattt
    11941 gcaatagctt ctgaaaccag agtcatcagt tctttttcct tggaaatccg cacctcttgc
    12001 ttagttggat gcacattgac atccgctaga taagggtcga tatggatgtg aatgacagcc
    12061 agtggaaaac gtccaaccat aagcttgctt ccaaaaccat ccaaaatagc acgattgagc
    12121 aggaagttct taatataacg gccattgatg aagaggctga tataattgcg gttagcccga
    12181 gtcaactcag gcaaggacac aaaacctgaa atttcgaaat ctaggtcaga gttctcaatt
    12241 tcaatcatct tcttggcact gaccaaaccg taaatccctg cgattgcttg gcgcaattga
    12301 ccagtccctg ctgtccgcgt catttccttg ccatcactaa tcaagctaaa agaaatctca
    12361 ggatgggcca agcccagacg gttgacaata tcaatgatat gagacaactc cgcttgctgg
    12421 ctcttcatat acttgagacg ggcaggcgtg ttgaaaaaga gatcctccac acaaaccttg
    12481 gttcccacag gactagtcgc tgggatgact tcctcaactt cacccccacg cgcgactaac
    12541 ttggttccat gactagcacc atccaccgcc gttaacagag tcaagacact aacagacgca
    12601 atagaaggca aggcttcacc acgaaaacca agcgtccgaa tccgaaagag atctgcttga
    12661 ttttttatct tactggtcgc atggcgacgc agggccaact ccacctcatc gtgggcaatt
    12721 ccatgaccgt tatccgtgat ttgaaccttc ttgagaccag cttcctcaat ctcaatgata
    12781 atctgactag agcccgcgtc aatggcattt tctaccaact ctttgaccac actggcagga
    12841 cgttcaatga cctctccagc cgcgatttgg tttgccagca tctctggcaa ttcaataata
    12901 tgagacatct ttcagcccct ttctgtatct attttcctag aaattgatta gtgctattat
    12961 accatatttc agaaagaaca gaaaagctcc accacatagg aacccaatcc ctccacatag
    13021 acaaagtccc ttgccagagg gagtaaaata gtggtatact caataaaaat caaagaacaa
    13081 actagaaagc taggctccta aaagggagaa agtctatgaa aagattagcg gacggcgttt
    13141 tgagcagact tcaattgttg caggacaagt agacggagag tttatagccc ctatgattta
    13201 caaggaaagt atgatgagca acttcccata ccgattccaa gaaggtcgct atcaggaata
    13261 ttccgttcgg tcaatttatt tttaatagaa ctgatgatgt ccgatataat ttgagaacct
    13321 ttctcacttg tatctgttgt aatactccac aaagtttcaa tctcaccgtc atttgaaaga
    13381 acagtaatct taatatttgt accacctagg tcaatactaa tcaacttctt atctatttcc
    13441 agatccttta tacaatcacc tgcgtataaa tttctaactc ttcatagttt tgttcagtac
    13501 tatcgtctgt gatgactgct gtgacttttt tcaaggaaca gatgaagcaa aatcttctcc
    13561 ccaaatcttt gaagagtcaa tgagtaagtc cgtttctggg gaacgctcca aggctagttt
    13621 tttagtaaaa tactcctcga aagaagaggt tattacacgt ccatccacga taccgttacc
    13681 actaaaaaca tcttgctaaa gtacatccta tccatcaaag cattggtaat ctttccgaca
    13741 aaagcttggg ttgtctcacg aatctcacca ccaagaagaa caaccttaaa gatatcactt
    13801 ttctttttca gttatagtca ttatgctttt gaatgaacat aaacatttct aatatatact
    13861 aaattctttc aatccattat ccgtataaaa aatataagaa aacaaaaaat aaggtatgct
    13921 aatagcaatt aacactaatt caatattcaa atcaataagc gttcctgcaa ctaaactacc
    13981 aattggcatt ccaaaagaaa taatagaatc aatggtagta tttaccctac caagaagttg
    14041 atgtggaggt atttgttggt acaacgaatt aaaaataata ttgaagactc ctttagacat
    14101 aaaacaaacg aaaaataaaa taagtgataa agtatagtct tttataacaa ttgctactaa
    14161 ccaacttgag ccgttcaaaa aaagaaatac accaacaatt tgattcgatt ttaaatattt
    14221 tattacgatt ggcgctagca tatttcccaa tataccaccc aaaccagcaa tagttaaaaa
    14281 aataccataa aaaatcggac catcaaaata tcgaatagaa aaaatcggta caactactgt
    14341 ctgaaatgaa taaaaaaagt ttataagcgt taaagaaata ctggttttaa ataataattt
    14401 attatttaa
//
LOCUS       DAVKHZ010000055        14004 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000055 DAVKHZ010000000
VERSION     DAVKHZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.055"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(21..872)
                     /locus_tag="VWW99_001527"
     CDS             complement(21..872)
                     /locus_tag="VWW99_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9539344.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            1199..1591
                     /locus_tag="VWW99_001528"
     CDS             1199..1591
                     /locus_tag="VWW99_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539345.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            1613..2404
                     /locus_tag="VWW99_001529"
     CDS             1613..2404
                     /locus_tag="VWW99_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539346.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            2397..3038
                     /locus_tag="VWW99_001530"
     CDS             2397..3038
                     /locus_tag="VWW99_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539347.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            3042..3674
                     /locus_tag="VWW99_001531"
     CDS             3042..3674
                     /locus_tag="VWW99_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9539348.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            3702..3989
                     /locus_tag="VWW99_001532"
     CDS             3702..3989
                     /locus_tag="VWW99_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539349.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            4135..6648
                     /gene="secA"
                     /locus_tag="VWW99_001533"
     CDS             4135..6648
                     /gene="secA"
                     /locus_tag="VWW99_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9539350.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            6729..7760
                     /locus_tag="VWW99_001534"
     CDS             6729..7760
                     /locus_tag="VWW99_001534"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9539351.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            7762..8793
                     /locus_tag="VWW99_001535"
     CDS             7762..8793
                     /locus_tag="VWW99_001535"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9539352.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            8832..9200
                     /locus_tag="VWW99_001536"
     CDS             8832..9200
                     /locus_tag="VWW99_001536"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9539353.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            9190..10293
                     /locus_tag="VWW99_001537"
     CDS             9190..10293
                     /locus_tag="VWW99_001537"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9539354.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            10312..12327
                     /gene="recG"
                     /locus_tag="VWW99_001538"
     CDS             10312..12327
                     /gene="recG"
                     /locus_tag="VWW99_001538"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9539355.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            12644..13624
                     /locus_tag="VWW99_001539"
     CDS             12644..13624
                     /locus_tag="VWW99_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9539356.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         4125 a         2548 c         3118 g         4213 t
ORIGIN      
        1 atgatgagag ttattacaaa ttataggttt gaaaaatcat taatttcttt ttcgagtatt
       61 ttaactaatg cctcttcttt ggttagtcgt aataaagtta ttcctttatc aatcaaatcg
      121 gagttatgtt gtagtattcc caagcgtact tgagagaatc caagaatatc gtatattttt
      181 aattcttctg ctaataccaa aagttgtagg gtgattgttt cacattcttt gaataaatga
      241 tgttgaaaat atacagtgga gagattggct aaaaaagatg ataaaaatgc ttttgtttct
      301 cggaagtatt tatatttctc gatggttttc ataattctat caataagaag gggaagattt
      361 tcagaaggaa agaaaaagag aatggttcca agcaatttca aatcactaat gtaccatgta
      421 tccataggtt caagatagtc ccaaatttct tcatacaaat ctttcaagac gtggttgttt
      481 ttgaatcctt tagttcgtaa ctctgtgaca atttttgtat gacgatagat attttgaata
      541 ggaacatcgt gatgtgtctt aagatatttt tgacatttct cagtgagttc aaccattttt
      601 ctagtatctt gaaaagtaga cggattttgg ctctctacaa taatatctcg ccttttacta
      661 ggatggtatt cgttgcatat atacttgaat tctgccaagc tcatatcaat ttgttcaaga
      721 agaaatacca tgttttcaaa actgggaata actttaccgt gttcaatatt tgttagagtt
      781 gttcgatgta tgacttgtcc acatacatct tgttgggtca atccttttga ctttcgaatt
      841 tctttaaaaa tttgtccata atcccatctc atattgttcc tcctaggtaa atgtgaatat
      901 tttctacatt tataaaaaac gtagaaaaag gtggtattat tatatcaaaa aggagggaaa
      961 aagtcatggg ctttaaaaaa tatttgaaga atttaccgaa aaactctgga tttttgattt
     1021 ggagttggat tcaacttatc tggtttgaaa catggttttg gggataaaga aattagagtc
     1081 tatatataat aagtaaagga ggaatttgta tgatatacca tcgtttagaa taagtctata
     1141 tttaaaatag aagttataag gaatcttctt gaataaaaaa tgaaaggaaa cagaaaaaat
     1201 gaaaattaag aaattattga aaatggttat tcctgtttta atgataagtg ctgttggaac
     1261 tactttcgta gaagcaaatc agataggtgc ttttagtaat tttgttatta ctaccagtta
     1321 taagagaaca ggttatttga ctaaagaaaa tgaaggtgcg gaatatatca tgaacttaaa
     1381 tccttgtaga aatttacatc ccatgactgt taagcatcgt atagtaaatt ctaatggaga
     1441 agctcgtagt ggagaatcgt taacaacctg tggtactcgt tcaactcatg gtaattgggc
     1501 aacggttggc tatgtttatg cagcagatat ggcaagacaa aattggtggg atttatcagc
     1561 tgccatttca ggtagttggt caccaaatta attcgtaaag taggtataat ttatgaagat
     1621 tcctctctta actttggcaa ggcataaatt tgtttatgtc ttgcttactt tgctttttct
     1681 tgctttggtt tatcgtgacg ttttgatgac ttatttcttt tttgatattc atgcgcccga
     1741 tctagctaaa ttcgatggac aagcaattaa aaatgactta ttaaaatcag cattagattt
     1801 tcgtattctc cagttcaatc taggttttta tcaatcattt attattccaa tcatcattgt
     1861 tttgctaggt tttcaatata ttgagctgaa aaataaagtt ttacgattga gtattggaag
     1921 agaagtgagc tatccagggt taaaaagaaa gttgactttg caagttgcaa gtatcccttg
     1981 tttgatatat ttagtgactg tgctgacaat tgcaattata acctatttcc ttgggacttt
     2041 ttctcctctt ggatggaatt ctctattttc tgatggaagt ggtttacaaa gactcctaga
     2101 tggagagata aaaagctatt tgttctttac ttgtgtcctg ctaatcggta ttttcatcaa
     2161 tgcaatctat tttttacaaa tagttgatta tgtggggaat gtgactcgtt cggcaatcac
     2221 ctatttgatg tttctttggc ttggttctat gctgctctat agtgccttgc cttactatat
     2281 ggttcctatg acgagtttga tgcaagctag ctatggggat gtaagtttga tgaaactctt
     2341 tactccttat atcctttata ttgtccctta catggtgctt gaaaaatatg aagataatgt
     2401 ttaagaattt taacaatatt ttgctaaaga gaaagattgt tttactactt cgtatagttc
     2461 tgatgatgat tttgataaac catctattgt caacagcggt tcaaaagcag gatgctgtta
     2521 tctttttcaa gagagaattg atttcaattt tttcctataa tgactattct gaagcgaatt
     2581 tagaaatccc caaactcttg ttaaaccttt cgcttttcat ggtaggatgg ctctctgtca
     2641 ttttacttga aagtgatttg gcagaccatt accatcactt gattcgctat caatcaagct
     2701 cctttttcga ttatacaagg aaacgattgg ttgtcatttc taaatttttt actcaagatt
     2761 tgtttgtctg gttccttggt ttacttccgc taggaattca tttcaaaaca gtcgcacttt
     2821 tctttttact tgctcagtta atgatgttgt acttactact gtcttatctg atagcactga
     2881 ttagtgcggg cgctggtttt tccttttttc tctatttttt agtatttgtg ggacaagaat
     2941 ggatgatgga tcatattgta acagtgtatt tactactctt aagtttatta gttatgttga
     3001 ttgttagtcg cttggaagag aaatttaaga aaggataaac gatgagactt gaaattataa
     3061 atggacagaa aatttatggg aaaagaccta ttttaaatca gttgaatttg gtgtttcaat
     3121 caggaaaaat ttatggactt aaaggtgata atggatctgg caagacggtt cttttaaagg
     3181 tacttgctgg ctatattaag cttgacaaag gaaaagttct tcaagacggg aaagtctacg
     3241 ggataaaaaa tcattatatt caggatgcag gaattttaat tgaaaaagtc gagtttttat
     3301 ctcatttatc cctgagagaa aatttggaac tgttaaggta tttttcatct aaagttacgg
     3361 aaaaaagaat tgcctattgg attcaatact atgatttaca ggaatttgaa gacgttgaat
     3421 accgtcattt atccttggga acaaagcaaa aaatggcctt gattcaagcc tttatttcct
     3481 ctccttctat actctttctc gatgaaccta tgaatgcttt ggatgagaag agtgtgaggt
     3541 taaccaaaca ggtcatttta tcttacctga aaaaagaaaa tggtctggtt atcctgacgt
     3601 cgcacatatc ggaagatatt tcagaccttt gtacagatgt attagttgtc gaaaatggac
     3661 atatacaaat gtaaaggata tacaatccta ggagatggct tatggcacat ctaaaatcat
     3721 ttattacacg ctattccaag gtttatattg gtttagttct gctgatctgg ctgtctttct
     3781 tctttatccc ttgggataga gcgattctgg gggtaaggat tgacatcttc atcatacaga
     3841 aaatcttgct agcttttgga attctgtcca ttctcatggc cttgctgtcc aagaaagtca
     3901 gtctctttgt ttttggactg atttgctgtc tttctctttg gattaactta tttatcacat
     3961 ttgccatttt gccgattttt ggcaattaaa cagtcataaa agtcggagag gttagcttga
     4021 aaactaacct ctttttcctt ttcaaaatgg ggattcttcc ttgaaaataa tcagtaattg
     4081 tgctaaaatt aaaggaacat tctaaaatat tcggaattta aagtaaggaa aaacatggct
     4141 aatattttaa aaacaattat cgaaaatgat aaaggagaaa tccgtcgtct ggaaaagatg
     4201 gctgacaagg ttttcaaata cgaagaccaa atggctgctt tgactgacga ccaactaaaa
     4261 gcaaaaacag ttgaatttaa ggaacgttat caaaatggag aatcactgga ttcattgctt
     4321 tacgaagcat ttgcggttgt ccgtgaaggt gccaaacgtg tcctaggtct cttcccttat
     4381 aaggttcagg tcatgggggg gattgttctt caccatggtg acgtgccaga gatgcgtaca
     4441 ggggaaggga aaaccttaac tgcgaccatg ccggtatacc tcaatgccct ttcaggtaaa
     4501 ggggttcacg tagttacggt taatgaatac ctgtcagaac gtgacgcgac tgagatgggt
     4561 gaattgtact cttggcttgg tttgtcagta gggattaact tggctaccaa atctccaatg
     4621 gagaaaaaag aagcctatga gtgtgatatt acttactcaa ctaactcaga aatcggattt
     4681 gactaccttc gtgacaacat ggtcgttcgc gctgaaaaca tggtacaacg tccgcttaac
     4741 tatgccttgg tcgatgaggt tgactctatc ttgattgacg aggctcgtac acctttgatt
     4801 gtatcaggtg ccaatgcggt tgaaaccagt cagttgtatc acatggcaga ccactatgta
     4861 aaatctttga acaaagatga ctacatcatc gatgtgcagt ctaagactat tggtttgtct
     4921 gattcaggga ttgacagggc tgaaagctac ttcaaacttg aaaacctcta tgacatcgaa
     4981 aacgtggctt tgacccactt tatcgataac gcccttcgtg ccaactacat catgcttctc
     5041 gatattgact atgtggtgag cgaagagcaa gaaatcttga ttgtcgacca atttacaggt
     5101 cgtaccatgg aaggtcgtcg ttattctgat ggattgcacc aagctattga agccaaagaa
     5161 ggtgtgccaa tccaggatga aaccaagaca tctgcctcaa tcacgtacca aaaccttttc
     5221 cgtatgtaca aaaaattgtc tggtatgacg ggtacaggta agactgagga agaagaattt
     5281 cgtgaaatct acaacattcg tgttattcca atcccaacaa accgtcctgt tcaacgtatt
     5341 gaccactcag accttcttta tgcaagtatc gaatctaagt ttaaagcggt tgtcgaagac
     5401 gttaaggctc gttaccaaaa gggtcaacct gtcttggttg gtacagtagc ggttgaaact
     5461 agtgactaca tttctaagaa attggttgca gctggtgttc ctcacgaagt cttgaatgcc
     5521 aaaaaccact atagagaagc ccaaatcatc atgaatgctg gtcaacgtgg tgccgttacc
     5581 atcgcaacca acatggcggg tcgtggtacc gacatcaagc ttggtgaagg tgttcgtgaa
     5641 cttggaggac tttgtgttat tggtacagaa cgtcatgaaa gtcgtcgtat cgataaccag
     5701 cttcgtggac gttcaggtcg tcaaggagat ccaggtgagt cacaattcta cctatctctt
     5761 gaagatgatt tgatgaaacg ttttggttct gaacgcttga agggaatctt tgaacgtctc
     5821 aacatgtctg aagaggccat tgagtctcgc atgttgacgc gtcaggttga agcagctcag
     5881 aaacgtgtcg aaggaaataa ctacgatacc cgtaaacaag tccttcaata cgatgatgtc
     5941 atgcgtgaac aacgtgagat tatctatgct caacgttacg atgtcatcac tgcagatcgt
     6001 gacttggcac ctgaaattca gtctatgatc aaacgcacga ttgaacgtgt cgttgatggt
     6061 catgcgcgtg ccaaacaaga tgaaaaacta gaggcaattt tgaactttgc taagtacaac
     6121 ttgcttcctg aagattctat tacgatggaa gacttgtcag gcttgtctga taaggccatc
     6181 aaggaagagc ttttccaacg ttccttgaag gtttacgata gtcaggtttc aaaactacgc
     6241 gatgaagaag cagttaaaga attccaaaaa gttttgattc tacgagtggt ggataacaag
     6301 tggacagatc atatcgatgc ccttgatcaa ttgcgtaacg cggttggact tcgtggctat
     6361 gctcagaaca accctgttgt tgagtatcag gcagaaggtt tccgtatgtt taatgatatg
     6421 attggttcga ttgagtttga tgtgacacgc ttgatgatga aagcacaaat tcatgaacaa
     6481 gaaagaccac aggcagaacg tcatatcagt acaacagcga ctcgcaatat cgctgctcac
     6541 caagcaagta tgccagaaga tttggatttg agccagattg gacgcaatga actttgccca
     6601 tgtggttctg gtaagaaatt taaaaactgt cacggtaaaa gacaataaaa tgagatagtt
     6661 tagaggcgga tatcttgtga aaagtaaatt tttactgggt atccgtttgc tttataagga
     6721 gatgagttat ggtatttaca gcaaaaagct ctaaaataaa tatagaagaa gttcgtgcct
     6781 tgtcaaaatt agaaggtcag gctttggaga ggaaatcaca gcgagatcaa gagctagaag
     6841 ccattatacg tggagaagac cagcgaattc tcttggtaat cgggccatgc tcatctgaca
     6901 acgaagaagc tgtccttgaa tacgctaagc gtttggcagt cctacaagaa gaagtggcag
     6961 atcgtatctt tatggttatg cgtgtttata ctgccaaacc ccgtaccaac ggagatggct
     7021 ataagggctt gattcaccag cctaacgcga cagaagcgcc tagtcttatc aatggaatca
     7081 aagccgttcg ccatcttcac tatcgtgtca tcacagaaac agggatgaca actgctgatg
     7141 aaatgcttta tcctgaaaac cttccgcttg tagatgattt gatttcttac atggcagttg
     7201 gtgcccgttc agttgaagac cagcaacacc gctttgtggc aagtggggca ggattttcta
     7261 ctggttttaa aaatccaacc tctggaaatc tcaatgtcat gtttaatggg atttatgctg
     7321 ctcaaaacaa acaaagtttc cttttcttag gaaaagaagt agaaacaact gggaacccgc
     7381 tttcacacgc tattcttcgt ggtgctctta atgagtatgg aaaaaatatt cccaactact
     7441 attatgacaa tttaattgat accattgccc agtatgagaa aatgggcttg gaaaatcctt
     7501 ttatcatcat tgataccaat catgacaatt ctggtaagca gtatattgaa cagatccgaa
     7561 ttgtccgcca gaccttgatt aaccgtgctt ggaatgaaaa aattaagcag ttcgttcgtg
     7621 gttttatgat tgagtcttat ctggaagatg gtcgacaaaa tgagccagaa gtatttggta
     7681 agtctatcac agacccttgc ctgggttggg ataacacaga agctcttgtc agagaaattt
     7741 acaaaacgtt aggagaataa gatggcattt attgaaaaag gtcaagaaat cgatatggaa
     7801 gtcatcaagg ctgaaaccca attgtctgcg gaagccttga gactcaagga aagccgtgac
     7861 agggaattgg cagatattat ttcaggggaa gatgaccgta ttctcttggt gattggtcct
     7921 tgctcttctg ataatgaaga ggcggtcttg gaatatgctc gccgtttatc tgccttgcaa
     7981 aagaaggtag cggataagat tttcatggtc atgcgcgtgt atactgctaa gcctcgtacc
     8041 aatggagacg gctataaagg attagttcac cagccagata cttctaaggc tccaagcctg
     8101 attaatggct tgcaggctgt gcgccagttg cactaccgcg tgattacaga gactggtttg
     8161 acaacggcag atgagatgct ttatccgtca aatctgatct tggtggatga cttggtcagc
     8221 taccatgccg ttggagctcg ttctgtggaa gaccaagagc accgctttgt ggcttctggg
     8281 attgatgcac cagtagggat gaaaaatcca acctcaggaa atttgggtgt tatgtttaac
     8341 gccatctatg ctgctcaaaa caagcaaacc ttcctttatc atgggcagga agttgagaca
     8401 tcaggtaatc ctttggccca tgttatcctc cgtggagcag tcaacgagta tggcaattat
     8461 atgccgaatt actactatga aaatctactc caagccattg aacgctatga aaccatggga
     8521 cttgaaaatc cttttatcct cattgacacc aaccatgata actcaggcaa gcaatatatg
     8581 gagcagattc gaattgttcg ccagaccttg cagaatcgtg attggaatga gaaaattaaa
     8641 aagacggttc gaggatttat gattgaatct tacctagcag atggtcgtca aaaccaacca
     8701 gagatctttg gttgctctat tactgaccct tgcctaggtt gggaaaatac agaggccttg
     8761 gtagaagaga tttatgttac cttgacaaaa taagtgaaaa ggatggagtt ggggaatctc
     8821 aactcctttt gatgagaatg atagttggac acggaattga catcgaagaa ttggcttcga
     8881 tagaaagcgc agttacacga catgaaggat ttgctaagcg tgtactgacc gctcaggaaa
     8941 tggagcgctt caccagtctc aaaggacgca ggcaaataga atatttagct ggtcgctggt
     9001 cggctaagga ggccttttcc aaggctatgg gaacgggcat tagcaagctc ggttttcagg
     9061 atttggaagt cttgaacaat gaacgtgggg cgccttattt tagtcaggca ccattttcag
     9121 gaaagatttg gctgtctatc agccacaccg atcagtttgt gacagccagt gtcattttgg
     9181 aggaaaatca tgaaagctag tccacataga ccaaccaagg ctctgattca tctgggagct
     9241 attcgacaaa atattcagca aatgggggct catatccctc aaggaacgct caagttggct
     9301 gtggtcaagg ccaatgctta tggtcatgga gctgttgccg ttgccaaggc aattcaagat
     9361 gatgttgatg gcttttgcgt ttccaatatc gatgaagcca ttgaactcag acaagctgga
     9421 ctcagcaagc caatcctcat tttaggagtt tctgaaatcg aagctgttgc tctagctaaa
     9481 gaatatgact tcaccttgac agtggctgga ctggagtgga ttcaagcact cttagataag
     9541 gaagtggacc taactggatt gacagtccac ctcaagattg attcagggat gggacggatt
     9601 ggttttagag aggcaagtga ggttgagcag gctcaagatt tgctccaaca acacggtgtt
     9661 tgtgttgaag gaatctttac ccactttgct actgctgatg aggaatcaga tgactatttt
     9721 aatgcccagt tagaacggtt taaaactatt ttagctagta tgaaggaagt tccagagctg
     9781 gttcatgcta gcaattctgc aacgactctt tggcatgtag agactatttt caatgcggtt
     9841 cgtatgggag atgccatgta tggcctaaat ccaagtggag cggtcttgga tttgccttat
     9901 gatttgatac cggccttgac cttggagtct gctctggttc atgtcaagac agttccagct
     9961 ggagcttgca tgggctatgg agcaacttat caagcggata gcgagcaagt catcgcgacc
    10021 gtgccaatcg ggtatgcaga tggatggaca agagacatgc aaaatttctc tgtcttggta
    10081 gatggccaag cttgcccaat tgtcggcagg gtttcgatgg accaaatcac tattcgattg
    10141 cctaagcctt acccactcgg aaccaaggta accttgattg gctccaatgg ggataaggaa
    10201 atcactgcaa ctcaggtagc gacctaccgc gtaaccatta actatgaggt ggtttgcctc
    10261 ctcagcgacc gtattccgag agaatattat tagaaaagaa aggagtggag catgaatcta
    10321 catcaaccct tgcatgtctt gcctggtgtg ggaccaaagt cagcagaaaa atacgccaaa
    10381 ctaggaattg aaaacttgca agatctcttg ctctactttc ctttccgtta tgaagacttc
    10441 aaaaccaagc aggtgctgga gctggaagac ggtgagaagg cagttctttc tggtcaggta
    10501 gtgactcctg ctagtgtcca gtattatggt ttcaagcgca atcgcctgcg ttttagtctc
    10561 aagcagggag aggtcgtttt tgcggtgaat ttctttaacc agccctatct ggctgataaa
    10621 atagagttgg gagcaaccct tgctgtcttt ggaaaatggg accgcgctaa ggctagtctg
    10681 actgggatga aggttctggc tcaggtagaa gatgacctcc agcctgtcta tcgtctggct
    10741 cagggaatca gtcaggccag tctggttaag gtcatcaaga cggcttttga tcagggactg
    10801 gacctcttga tagaagaaaa tctgccccag tctttactag acaaatacaa actcatgtcc
    10861 cgttgtcagg cagtccgagc tatgcatttt ccaaaggatt tggcagaata caagcaggct
    10921 cttcgccgta taaagtttga ggaactcttt tatttccaaa tgcagctgca gatgctcaag
    10981 tctgaaaata gagttcaggg aagtggtctg gttctgaatt ggtctcagga aaaagtgaca
    11041 gcagttaaag taagtcttcc ttttgccctg acccaagctc aggaaaagag tttgcaggaa
    11101 attttaactg atatgaagtc cgaccaccac atgaatcgtc tcctacaagg ggatgtgggg
    11161 agtggaaaaa cggtagtcgc tggcttggcc atgtttgcgg cagtgacagc aggttatcag
    11221 gctgccctaa tggtaccaac agaaatcctc gcagagcaac actttgagag tttacagaac
    11281 ctttttccca atttgaaact ggctctcttg ataggttcct tgaaagctgc agaaaagaga
    11341 gaggtcttgg agaccattgc caagggtgag gctgatttga ttatcggaac tcacgctctg
    11401 atacaagatg gggtggagta tgctcgtctt gctttgatta ttatcgatga gcagcaccgt
    11461 tttggtgtag ggcaaaggcg tattttacgg gaaaaaggtg acaatccaga tgtcctcatg
    11521 atgacggcga ctcccattcc acggacgctt gccatcacag cctttggaga tatggatgtt
    11581 tccattatcg accagatgcc agcaggtcgg aagcctattg tgacgcgctg gatcaaacat
    11641 gagcaactac ctcaggtctt gacttggtta gagggggaaa ttcaaaaagg ttcccaagcc
    11701 tatgtcatct ctcctttgat tgaagaatca gaagctctag atttgaaaaa tgccattgcc
    11761 ttatcagagg agttgacgac tcattttgca ggcaaggcag aggtggctct tctacatggt
    11821 aggatgaaga gtgacgaaaa agaccagatc atgcaggatt tcaaggagag aaagacggat
    11881 attctggttt cgacgacggt tattgaggtt ggggtcaacg ttcccaatgc gactgtcatg
    11941 attatcatgg atgccgatcg cttcggtctc agtcaacttc accagcttag aggtcgtgtc
    12001 ggtcgggggg acaagcagtc ctacgctgtt ctcgttgcta atcccaagac ggattctggg
    12061 aaagaccgca tgcgtatcat gacagaaacg accaatggat ttgtccttgc ggaggaagat
    12121 ttgaaaatgc gtggttctgg tgagattttt ggaaccagac agtcaggact tccagagttc
    12181 caagtggctg atattatcga agattttccg attttagaag aagcaagaaa ggttgctagc
    12241 tacattagtt ctatagaagc ttggcaagaa gatccagagt ggcgcatgat tgcccttcat
    12301 ctggaaaaga aagaacatct ggattaagct ttctctaagg aaaacttaag ctagttttta
    12361 ggtttggctc ttatactaaa gtcatcaaaa agaaacgagg actctcatat gacagtaacg
    12421 attaaagtaa attaccaaac cactttccaa aagaaggaag caaaaaacta gtataaacag
    12481 aagagagagc gaaatgctct tttttcgttt ctaaaactac tttcagccca tcatcctaaa
    12541 agtaaagaat ctaaattcac tttctattta cccttctttc ttgcattgat tacatagata
    12601 tgctacagtt gtggtaacga ttacaaaata aaaggagcat gctatgaaaa atccagcttt
    12661 gctagaagaa attaagacct atagaggaag ggatgaggtt ccggaagact ttgatgcttt
    12721 ctgggatggg gaagtgaaaa atgtttccac acttccatcc taccacttgg aggaaagaga
    12781 tttccacatt cctcaagtca agtgctatga gttaacattt gaaggaagca aggaaggaaa
    12841 ggtctatgca cgcattgttc ttccaaagag tgaggagaag gttccattaa tcttccattt
    12901 tcatggttat atgggacgtg gctgggactg ggccgacatg ctgggcttca ccgtagctgg
    12961 ttacggtgtt gtttccatgg atgtgcgggg ccagtcaggt tactcacaag acggcttgcg
    13021 ttctccttta ggaaataccg tgaaggggca tattatccgt ggtgctatgg aaggtcggga
    13081 ccacctcttt tataaggatg tttatctgga tatttaccag ttggtcgaaa ttgttgctag
    13141 tctgtctcag gttgatgaga agcgtctttc tagctatggt gcctcacaag gaggggctct
    13201 agctctagtt gcagcagcgc tcaatcctcg aattcagaaa acagttgcca tttatccctt
    13261 cttgtcagac ttcagacggg tgattgagat tggtaatact agcgaggctt acgacgaact
    13321 tttccgttat ttcaagtttc acgacccctt ccatgaaaca gaggaggaaa tcatggcgac
    13381 ccttgcctat atcgatgtca aaaatcttgc ccatcgtatc caaggtgagg ttaagatgat
    13441 tacgggcttg gacgacgatg tttgctatcc cattacccag tttgcgattt ataatcgtct
    13501 gacctgcgat aaaacctatc gcatcatgcc tgagtatgct cacgaagcca tgaatgtatt
    13561 tgtcaatgac caagtctaca actggctctg tggaagtgag attcctttta aatatctaaa
    13621 ataaggagtc gactctaagc acaaaatctt aaaaattaca aacacgcata gtatcagggg
    13681 attaaaaaaa cttgatacta tgcgttttat catggaaata tagtaaaatg aaacaagaat
    13741 aggacaaaac gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gcaaatgtgt
    13801 actattctag tttcaatcta ttatatttat agaatttttt gttgctagat ttgtcaaatt
    13861 gcttaaaata atttttttca gaaagcaaaa gccgatacct atcgagtagg gtagttcttg
    13921 ctatcgtcag gcttgtctgt aggtgttaat acttttcaaa aatctcttta aaccacgtca
    13981 gcttcgcctt gccgtatata tgtt
//
LOCUS       DAVKHZ010000056        13781 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000056 DAVKHZ010000000
VERSION     DAVKHZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.056"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(60..509)
                     /locus_tag="VWW99_001540"
     CDS             complement(60..509)
                     /locus_tag="VWW99_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9539357.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(511..1269)
                     /locus_tag="VWW99_001541"
     CDS             complement(511..1269)
                     /locus_tag="VWW99_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9539358.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VWW99_001542"
     CDS             complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VWW99_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9539359.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3360..4100)
                     /locus_tag="VWW99_001543"
     CDS             complement(3360..4100)
                     /locus_tag="VWW99_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9539360.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VWW99_001544"
     CDS             complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VWW99_001544"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9539361.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5421..6356)
                     /locus_tag="VWW99_001545"
     CDS             complement(5421..6356)
                     /locus_tag="VWW99_001545"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9539362.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGCDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPKEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEVLPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6369..8765)
                     /locus_tag="VWW99_001546"
     CDS             complement(6369..8765)
                     /locus_tag="VWW99_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9539363.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRTYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8831..9145)
                     /locus_tag="VWW99_001547"
     CDS             complement(8831..9145)
                     /locus_tag="VWW99_001547"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9539364.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VWW99_001548"
     CDS             complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VWW99_001548"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9539365.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9926..11539)
                     /locus_tag="VWW99_001549"
     CDS             complement(9926..11539)
                     /locus_tag="VWW99_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9539366.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11730..11984)
                     /locus_tag="VWW99_001550"
     CDS             complement(11730..11984)
                     /locus_tag="VWW99_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9539367.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(11988..12242)
                     /locus_tag="VWW99_001551"
     CDS             complement(11988..12242)
                     /locus_tag="VWW99_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9539368.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12480..13577)
                     /locus_tag="VWW99_001552"
     CDS             complement(12480..13577)
                     /locus_tag="VWW99_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9539369.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILARLGIDSLAFGTEEVLD
                     YQKIADLYTEQGAEMENFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGYNIKLHPIQRQGAGYHSVDKDVNFASATALRQHQRDKDFLERFMPSVALFEQA
                     SKVSWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQVRESNLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         3670 a         3280 c         2725 g         4106 t
ORIGIN      
        1 gaaagcgttg ttaatttatt tatctcattt ttcagaaaat tcttttattt ctgtaaagtc
       61 tacgatactc gatgtgtttt tatataatga tacagtctga gaatcactgt tccgctagcc
      121 attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc cagtgcttta
      181 ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc tggaaccagc
      241 ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat acttaaaatc
      301 tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac attggtcggt
      361 tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc aggtaagagc
      421 atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa acttgctact
      481 gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg cgacagaagt
      541 tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca taccagagtt
      601 tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg gtacaaacgg
      661 catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt agcgagccca
      721 aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa agggaattcg
      781 gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca ctcctgcccc
      841 aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac taaaggtcgc
      901 agccagagtt acctgcaact tagtataggg aaggggttga gcttgcaagg ccgtcaattg
      961 cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa tctggttcac
     1021 atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc gcgaaatatt
     1081 ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc aatccacaat
     1141 cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt ctgagccact
     1201 tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta attcttttga
     1261 ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat ccggactcaa
     1321 aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat tatccatctg
     1381 cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga ctctagtctt
     1441 atttaggtct accttttcac ctgctctagg actttgttca acaaccatgc cttctacact
     1501 acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa tcccaacaat
     1561 ttgaatcaaa ttgttcttag taaactccaa gctggaacca atgtaactcg gcatggcaac
     1621 acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca catcataagt
     1681 cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc tggactcttc
     1741 ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg agattacttc
     1801 tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt tagctactgt
     1861 caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta gactttgctt
     1921 caagaccgtt ccagcctcac tctcattcga ctcttcttcc tcaattttaa tcaaattatc
     1981 tggaactttt ttctctttta attccgcgat aacatcagaa gatttccggc cgacataatt
     2041 actaatttgg aaggattgtt tgcctgatga gacaaccaga ttgattttcg ttccttcttt
     2101 tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca ccttttcact
     2161 agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg ccttggcctc
     2221 tgcaactgtc tgacctgcca catctggaat ggcaatggtt gcaggagttc tggatagtat
     2281 ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt aacgcatctt
     2341 aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag cctggcttgg
     2401 gtttttgatt gatttgtgtt ctgtctgcgc ttgaacctta ggaatagatg tcaaggtact
     2461 ttgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa agattaactt
     2521 actttcattt ctacgattgt aggacaagct actagacaag tccacataca tctctgaaac
     2581 cgagcggtag cgattggtca actttttagc agttgccttg ataataacat tttctaaagc
     2641 ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga aatgctggag
     2701 ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcaaca tctcatagaa
     2761 aataatcccc atggcataga tatcactctg cacagtcgcc ttcgaaccac gcgcctgctc
     2821 tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac ttgtctctgc
     2881 aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg tcaaaaggat
     2941 attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagc gcatagccaa
     3001 gagaatttgt cccatgatac ggactgcttc ttcattagaa agaggataat gttccttgat
     3061 atagcgtttg aggtctagtc cagcaacata ctccattgca agatactgtt gaccgtcttc
     3121 ctcaccaata tctgttatcc gaacgatatg aggatggtct agatctgcca tagctctcgc
     3181 ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc tcagaacctt
     3241 cactgccact tcttccccat ctaagattaa gtctttggct aggtagacat ccgccatgcc
     3301 tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc cgatttggat
     3361 cattctgcat cctcctcgtt catagaaaca agggcaaccg taatgttgtc taaacctcct
     3421 gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat atcactggtt
     3481 acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact attgagcaag
     3541 agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc atctttttgc
     3601 ccaatagact gggtgataat atttttttgt ggatgagctt ctgcctcttc tggtgtcaat
     3661 tgaccagcct tgagcaattc attaaccaaa gaatgatcgc tcgtcaactg atggtattct
     3721 tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc ctgattatca
     3781 ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc ctgaccaagc
     3841 tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg cacttcattg
     3901 actgtatcaa tctgggtatc aacccaagct acacccaggt ctgtgaccgc catttcacta
     3961 gcgatattcc ctgcgcgatg acctcccatc ccatcagcta aaataatcat ggtacgtcca
     4021 gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg accaacatct
     4081 gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg cgaaattgac
     4141 tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag ccgtctttca
     4201 tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga ctctctaaaa
     4261 aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta taagttatta
     4321 taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga atttcctgca
     4381 aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg cgcaaaagac
     4441 cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg tcaaaaaact
     4501 catgcacctt tctggcatcc aatttttgag tttgaacccg atctgcaact cccagacgtt
     4561 gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca gtaacctgac
     4621 ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca caggcatcaa
     4681 gcactcgctc atcaccttgt aaatcaagcg tcggagcaac cagctgactg gactcgtctt
     4741 ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc tgctccttaa
     4801 ccagaccagt ggttgctaaa agggaatcat tcgcctccaa caaggcttgg atttcctctt
     4861 ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg ctttcaaaga
     4921 tggcttttgc tctctcctct ccgtactctt ccttgagttt ggcaactagc caaactggga
     4981 gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata tctggccagc
     5041 cttcacgcaa gatacggcga aggacagcgt tgaccaattt ttcactgcct tttttacgga
     5101 gtttggctaa ttccactgct tcattgacca cagcatgatc tggaatcttg tccaaatagc
     5161 ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac tggtctctgt
     5221 cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc gttccataga
     5281 ctagctcggt cactaagccc ttgtctgctg cagagagctg actccccttg aggtgtttat
     5341 ttaaggcaat atttgaatat gcttgattca caaaaacatc ctctagcact gctagagcta
     5401 aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa tgtacgtcca
     5461 actccattga ggaaggaagc aatgtccatc ttaggcttac cagctggctg cacttgtttg
     5521 agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat agagagaatc
     5581 tcacctggat ttccctgacc ttctactggt aggacttcat aaatcttaaa gcggtcgccc
     5641 ttaaggaaag tatgggcaac aggccagggg ttcattccac gaatttggtt aaagagttga
     5701 cgattggttt tgttccagtc cagtttttct tcctttggct ttatatttgg agagaaggta
     5761 acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc aggcagagtg
     5821 tccaaaagca aatcacaacc aactagcgcc aatttttcaa acaaggtgcc aacattgtcc
     5881 tcatctgtaa tcggaatgct gcgacgagaa atcatatctc ctgcatccat ttccttaacc
     5941 atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata atggataggc
     6001 gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa gtccatgcta
     6061 tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc atctgctcct
     6121 agcttcataa gatcttccat ctctggactt ccagataatt tttcaggttg gtagatagat
     6181 agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt tttacgacca
     6241 acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc tgtcaaaagt
     6301 ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt tgtcatatct
     6361 tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag ctcactattt
     6421 tcccgttctt gagtcaaggc caggacctgg ttgagggtcg gccccagctc atcttctaaa
     6481 cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat cggttttggc
     6541 gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt catgacttca
     6601 taggtacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc aatcgtgaaa
     6661 tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata aaagccttcg
     6721 taatcttgat ccttggcaaa tcgaatagca tagtgctgcg gattgtagga ctgtatcaag
     6781 acttgacctg ctttttcagc ccgacctgct cgacctgcca cctgagtcaa gagctggaag
     6841 gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt tagaactccg
     6901 actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc aagtaaaata
     6961 tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt ctttcgagtc
     7021 gtatccacat ccatcctcag aatgcgagct tggggaaaga gttctgctag ctcatcataa
     7081 gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacagtt aggacagacc
     7141 tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt atccatatgc
     7201 aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc ccgacacatg
     7261 acaaagctag aataaccacg acgattgagc atgagaacca cctgctcttt tttaaccaga
     7321 cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt ttgtccgata
     7381 tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt ggcacgttgg
     7441 gttagacgta agtgttgata gacgcctttg ccagcacgcg cccggctctc taagctcggc
     7501 gttgcagatc caagtaccag agttgcttga ttatactgag cccgtaaaat agctacctct
     7561 ctggcatggt aacggggatt gctgtcctgc ttataagcca cttcatgctc ttcatcaata
     7621 atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc aacaacaact
     7681 tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcaccatt ggataatcct
     7741 gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt catctgagga
     7801 gtcaaggaaa tctcaggtac cagcaaaata gctgtcttgc ccttatccag ggcaccttgg
     7861 ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag tagaaaggga
     7921 ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg ttctggattt
     7981 aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg ttgaacttcc
     8041 ttttggacta tggtaacagc accttgatcc acaaagaagt tgacttgctc tcgcgagtag
     8101 gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata atctctcagt
     8161 tccaactttt tcttggcacg tgtagaaatc tcaacacctt ctaattgagc atggtcaacc
     8221 tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc cagaccaagc
     8281 aggccttttc tagtcaaacg catcatttca gcttgcttgg caaggtctag tgaagaaaag
     8341 gctagcgaat cttctgaacc aaacaggcgc actcgttctt cctgactcaa gccttccaga
     8401 ggatagagaa tcttgtcata gctagaattc agaaatcctg gaagcatggc cttgagaata
     8461 gaaattttgt aggagaagac agatttgcgt aactcctcag ccagccagag ttgttctggc
     8521 gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg ctccatctct
     8581 tctccatctg attgggactt caaaccaaga acaatccctt gaatcaggcg attaccctta
     8641 ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc ctccggaatc
     8701 ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat cttagctagg
     8761 gccatcttct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat aagattgagt
     8821 ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc tctttgattt
     8881 tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc actgcttcac
     8941 gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct tctaaagcgc
     9001 gaagagttga tttttcagac ttgaaacctt gagttgctgg ggcacctgct tccaattcgt
     9061 gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg tcgagcaagg
     9121 tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt tatcgggtag
     9181 ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca cagaagtgtt
     9241 ctgcttcaat cacacatttg acacgttcag cagctagggg cacctgatcg ttgacaatcg
     9301 cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt cgttgggcaa
     9361 tcacttctgc actatctgtt ccacgaccta ccaagcgatc ttgcaattca tccaaatctg
     9421 gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga agagcaccct
     9481 gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg acataggtca
     9541 gaggagttcc atagtagttg ccgacatatt ctgcgtattc caacatctgt ccttgacgaa
     9601 tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc acttctccag
     9661 gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca gaactctcaa
     9721 aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa acgattagta
     9781 agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat ttctctagaa
     9841 taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac aaaaaagcag
     9901 taatttttca actgctcttt cttatttatt tagcataatc tactgcacga agctcgcgaa
     9961 tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc ttacgaactt
    10021 tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc atgatacgaa
    10081 tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg ttagcaattt
    10141 cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga gcacctggac
    10201 gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc tcagcttcaa
    10261 catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg tacttacggg
    10321 ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg gctttcccga
    10381 tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt tcgctcgcca
    10441 tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt ccatatgaag
    10501 tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt ggcgcaccaa
    10561 tttcataagc agcagcctca ccgtattcac gaatcttatt gtcaatctct tgacggtttt
    10621 tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc aacatttcca
    10681 tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc accacttctg
    10741 gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga atgttacgac
    10801 cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact gttgagtttg
    10861 ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag atgtccttgg
    10921 ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga ctggcaatct
    10981 ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct gcctgagaca
    11041 gagcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct aattgctctt
    11101 cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt tcaagtgttt
    11161 gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc tctgtcaaac
    11221 gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc acttcttcac
    11281 ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga gacttgcttt
    11341 cacgtttagc ttcattaaca agtaaatccg cttcacgctc agcttgtcca cgtaaattag
    11401 ttgcttcttg ttcagcattt aaaagcatca actccgcagc ttcctgagat gatttcatct
    11461 tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa acagcaatcg
    11521 caagcgacat gatttccatg tttttacctc attttattgt tattccgaat gacatacatt
    11581 cttttacatt ctaccataaa aaagtgattt tcacaaacct aaaatagaat atgttttggg
    11641 gaatttggaa tacatttacc aaaataaact tgttgtttag aaatagtagt ttagtagaga
    11701 cttgagaaaa agcctacttt gcagtagact tagtaatgat ctttaaagga caagaaagcc
    11761 acgctatctc catccatcat ataaatcaag cgattttctg catcaatacg ccgtgaccag
    11821 gctccttggt aatcatattt gagtggctct ggttttccta ttcctgtaaa tggatcgcgt
    11881 tgaatatcct tgattaattt attgattctt tttagtgttt ttttatcttg gttttgccag
    11941 tagcaataat ctgcccaggc atcttctgta aacttgagta gcattcatca ctcctcaatc
    12001 acatggacct gagtgcttcc agcacggact tgagccattc ctcgcaaaac cttgtctgat
    12061 aactccttat tttgggcgat tctcagggtt tcctgaatac tgtcccattc gctctttgaa
    12121 agaactacaa tgtcctcatc tggattttta ttgaccaccg tcaaaggctc aaattcatca
    12181 tttaccttct tcatgtagtc ctttaaatga tttcggaatg ttgagtaaag gactgcttcc
    12241 ataaccatac ctcgttttag ctcttttcca ctattataca cgaaaagaaa gaaattgtca
    12301 ggaacttgta caagattttc ttttctatct atttatactc aatgaaaatc aaagagcaaa
    12361 ctaggaaact agccgcaggc tgtacttgag tacggcaagg cgacgttgac gcgatttgaa
    12421 tttgattttc gaagagtatt attcgtaaaa aatctcaaaa agcctacctt tcggtagact
    12481 tagtttgttt ctattctaat cggcactctt ccaaaatttt gctctgctat acttggcttt
    12541 cctagttggt aaatctggtc tgctttttgg gtcatagcat cccaaggttc tttgccaatt
    12601 cggctgacta gactgacctg acctttcaaa gacttgagat gttgcctgcc tttttcagta
    12661 aatccaagga catgaatcgc ttctggcaag ttactttctc taacctgcac caaaatatag
    12721 gtcaataggc gtctgacacg cgccttggtg taacgtttgg tagtcactaa ctcgaccaat
    12781 tcttccacag actgggctgt tttaatagct tctttaatgc gcacagccat ttcttgattg
    12841 acctgataga tggtggttag gtcgggattt gacaagattt gatagcggag caagggaaaa
    12901 tagtcttccc agctcacctt actggcctgc tcaaagaggg caacagaagg cataaatcgt
    12961 tctaagaaat ctttgtccct ctggtgctga cgaagggctg tcgccgaagc aaagttcaca
    13021 tccttgtcca cagaatggta gcctgccccc tgacgctgaa tcggatgaag tttgatgttg
    13081 tacccagcaa ccgccttggc gtaagcgagg gcaagaacat gattgggcgt attgcctgaa
    13141 aaatcaagac ctgcaaattc cttccacatg gcttgggtct tctggggata agaaagagag
    13201 tcaggcaaat tttccacaaa attctccatc tcagccccct gctctgtgta taagtcagcg
    13261 attttttgat aatctagaac ttcttctgtc ccaaaagcga ggctatctat gcccaaccga
    13321 gctaagatat ccacggcccc ttgaccgaaa aaatctgccg cctgaacgct gactaaaaag
    13381 ggcaattcca ctaccagatc cgctccattt tccagcgcca tctgggctcg tgtccactta
    13441 tccacgatag caggttctcc acgctgcatg aaattccctg acatagccac gattttcagc
    13501 ccctccgctt gatccagcag gtatttgtgt ccattatgaa aaggattgaa ctccgcgata
    13561 atacccgtga tggtcatagt aatctcctac ttctttagta tcaaattgag tcaattatat
    13621 tctgtaggct ccgtttttga gtagttgttt cctcaatgaa tttaattgac cattctttta
    13681 agtaattgtg tctactaaaa taccagccat ttatccactt atttattaaa gttttttcat
    13741 catcgttcaa catgttataa tattttcttt catttatctt c
//
LOCUS       DAVKHZ010000057        13586 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000057 DAVKHZ010000000
VERSION     DAVKHZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.057"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(116..2374)
                     /locus_tag="VWW99_001553"
     CDS             complement(116..2374)
                     /locus_tag="VWW99_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9539370.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2603..2833)
                     /locus_tag="VWW99_001554"
     CDS             complement(2603..2833)
                     /locus_tag="VWW99_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9539371.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3023..3706
                     /locus_tag="VWW99_001555"
     CDS             3023..3706
                     /locus_tag="VWW99_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9539372.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3706..4440
                     /locus_tag="VWW99_001556"
     CDS             3706..4440
                     /locus_tag="VWW99_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9539373.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4594..5451
                     /locus_tag="VWW99_001557"
     CDS             4594..5451
                     /locus_tag="VWW99_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9539374.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5593..6465
                     /locus_tag="VWW99_001558"
     CDS             5593..6465
                     /locus_tag="VWW99_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9539375.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     ATLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6747..7430
                     /gene="rpiA"
                     /locus_tag="VWW99_001559"
     CDS             6747..7430
                     /gene="rpiA"
                     /locus_tag="VWW99_001559"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9539376.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7444..8655
                     /locus_tag="VWW99_001560"
     CDS             7444..8655
                     /locus_tag="VWW99_001560"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9539377.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIYDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8657..9199
                     /locus_tag="VWW99_001561"
     CDS             8657..9199
                     /locus_tag="VWW99_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9539378.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9215..10024
                     /locus_tag="VWW99_001562"
     CDS             9215..10024
                     /locus_tag="VWW99_001562"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9539379.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEISNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10521..10658
                     /locus_tag="VWW99_001563"
     CDS             10521..10658
                     /locus_tag="VWW99_001563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539380.1"
                     /translation="MKESENDAPKLETPLREEPRLAPQTLPEASEVLENKREESKVEI
                     T"
     gene            10677..11479
                     /locus_tag="VWW99_001564"
                     /pseudo
     CDS             10677..11479
                     /locus_tag="VWW99_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963254.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11504..12016
                     /locus_tag="VWW99_001565"
     CDS             11504..12016
                     /locus_tag="VWW99_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9539381.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFCFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGSGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12670..13380
                     /gene="deoD"
                     /locus_tag="VWW99_001566"
     CDS             12670..13380
                     /gene="deoD"
                     /locus_tag="VWW99_001566"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9539382.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13430..>13586)
                     /locus_tag="VWW99_001567"
     CDS             complement(13430..>13586)
                     /locus_tag="VWW99_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539383.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSQA"
BASE COUNT         4012 a         2481 c         3078 g         4015 t
ORIGIN      
        1 agattcagtc tactatatat cataatggac aaagggcaag ctgtcaagaa attacagtta
       61 ttttagtaaa aaaagaagca gaaaaatttc attttctact ccttttctat aggatttatt
      121 tttctttttc agaactttta acttcagctt ttttggcaga tttaatctga atgtttccct
      181 tgctagtcat aactgctttg agatcttttt cgcttggatt ttcaaggtag tagtcagtga
      241 ttgtgtcctc aatatagtct tgaatagtcc gacgaagtgg gcgtgctccc atttttggat
      301 cataacctag gtcaaccaac ttttccttga ccttatcagt tacatccaaa cgaatgttgt
      361 tgctagagag gcgcttgtta acatctgcta gcatgagctc gacaatctga aggaggttat
      421 ccttgctgag agccttaaat tcgataatgc catcaaaacg gttcataaac tctgggctaa
      481 agaagttacc gagttcaccg aggacagaat tggtacgtcc ttctctagca gcaccaaatc
      541 caacgctagc ttcggtcttt cctgtacctg catttgaggt catgataatg atggcatcct
      601 tgaagctaac ggtgcgtcct tgcccgtctg tcaaacgacc atcgtccaag acttgaagaa
      661 acatgtgcat aacatctggg tgagcttttt ccacttcatc gagaaggatg agagaatatg
      721 gattgtggcg aactttttca gttaattgac cagcctcatc atagccaaca taacctggag
      781 gggcgccgac caacttagcc acactatgtt tttccatgta ttcactcata tcaaagcgaa
      841 tcatactatc agcagaacca aaaagttcga tagccagttg tttggaaagt tctgtcttac
      901 cgacaccagt tggcccaacg aagaggaagc ttccgattgg gcggttaggg gtaccaagtc
      961 cgacacgatt acggcgaata gccttggcaa tcttatcgac tgcatcatct tggccaataa
     1021 catgagactt gagatcttcg gctagatgga tgagttgaga ttgttctttc tctttcaaat
     1081 caccaacagg gatattggtt ttctgctcga taatgtgctc aatagttttc tcgctgatga
     1141 taggagtatc ctggtctgtg atctttttct tttgcatttc cttatacttg gcaatctggt
     1201 cgcggaagta ggccgccttc tcaaaatctt cttctcgtgt agcttgagac ttgagatttt
     1261 cagcctcaat caagcgctga tcaattactt taggatccac aaaattcaag gtcaagttca
     1321 tcttagaacc agcttcatct aggaggtcaa tggccttgtc aggcaagaag cgatcttgga
     1381 tgtagcgatt ggaaagagtt gcagctgctt caatcgcagc atctgtatat tgaacgtggt
     1441 ggtaatcttc gtatttcttt tgaatccctt tgagaatagt gattgtttcg tccaccgttg
     1501 gttcatcgac tttaacaggc tgcatacgac gctcgagggc agcatccttt tcaatgatac
     1561 ggtattcatt gagggtagta gcaccgacta gttgcagttc tccacgagca agggctggct
     1621 tgaggatatt tcctgcgtcc atattaccat cactcgcaga accagcacca acaatttcat
     1681 ggatttcatc gataaagagg atgatgtctt cacgtttgcg aatttcttcc atgagttttt
     1741 gcatgcgttc ttcaaattgt cctcgaatcc ccgttccttg aactaagcta accacatcca
     1801 gacggatgac ttgtttacct tggagtttat gtggcacatc gccatcgaca attttctgag
     1861 ctagaccttc gacaacggcc gtttttccga caccaggttc accgataagg acaggattat
     1921 tcttggttct acgattgaga atctcgatga cacggataat ctcatcgtcg cgcccaataa
     1981 cggggtcaat gtctccacga cgggcaattt cagttacatt aataccaaat tcttccagca
     2041 ggcctttttc ttggctaggt ggcggagttt gagcagatcc acgattttgg gaaccataac
     2101 cgccgtttcc accgtaacct ccacctgatt gggttggggg aataggagga gtattgctag
     2161 aaggtctgaa attgtttaga tcattgaaga aatcaccaaa gggatcgaag tcacgattgt
     2221 tcagatccgt catacctttg aagaggctat tgttaggatc tgttttgata atcttatagc
     2281 agttttgaca gaggtcaatt tgtttttgtt ttccattgag attggtgtaa agatgaattg
     2341 ttgagtcgtt aattttacag ttttgacaaa gcatacatct acctcatttc tttctttagc
     2401 ctgacttgtt taaaggtcaa aaatagtcaa attctatact ctcattataa caggattggg
     2461 gtgtaaagca agtaaaaaga ttgaagcttt gagaagttgt tacaatgaaa atttttgtga
     2521 atttggacta taaaaaaatc ctatttgtga gacaaaaagg attttactta gacatgcagg
     2581 gacaatccca gcttgtttta gattagtaga gaagttcgta aatttccatt gcgattaagt
     2641 caatgctatc aaacgtatac gtttcgcgat cttctacgtc acgaagggta aagattgggc
     2701 cgttttcttc gtcgtggctg tacgcaactt ctgcaacaac aactccttcg cgttcaaaat
     2761 tacgttttaa atttccgcca tcgtttgcca ttgcttcaag gcggttgatg attctaacca
     2821 aatgtgattc cattttgatt ctccttcatt tcttatcgtt actattttac cataaaggag
     2881 gccaacttga ctagaaaaaa ctaagatttc tcgctaattc tgctagttta aagattttta
     2941 aataaattgg ataaaaaatt taaattttaa ttcaaaacat gttataatca tacagtattc
     3001 tattttagaa agcagtgtga ctatgaattt ttctttttta cctaagtatt taccttattt
     3061 taactatggg gctgttgtga cgattcttat ttctatctgt gttatctttt tgggaactat
     3121 tttgggtgtt gtcttggctt ttgggcaacg ttcaaagttt aaaccgcttg tttggttggc
     3181 caacttgtac gtttggattt tccgtgggac accgatgatg gttcaaatta tgattgcctt
     3241 tgctcttatg catatcaatg ctccgactat tcagattgga attttaggtg ttgatttttc
     3301 gcgtctgatt ccagggattt tgattatctc tatgaatagt ggtgcttatg tttcggagac
     3361 tgttcgtgcc ggaatcaatg cggttccaaa aggtcagcta gaggcggctt attcgctagg
     3421 gattcgtcct aaaaatgcga tgcgttatgt gattttgcca caagcaatca aaaatatctt
     3481 gccagcattg gggaacgaat ttatcaccat tatcaaggac agctccctct tatcagctat
     3541 tggggtcatg gagttgtgga atggggctac aacagtttct acaacaacct atctaccttt
     3601 aacaccgctt ttatttgcag cattttatta cttgattatg acctctattc tgacagtagc
     3661 cttgaaagct tttgaaaaac atatgggaca aggagataag aaataatgac agaaaccttg
     3721 ataaaaattg aaaatttaca taaatctttt ggaaagaatg aagtattgaa gggcatcaac
     3781 ctcgagatta aaagaggaga agttgtcgtt atcatcggtc cttcagggag tgggaaatct
     3841 accttgcttc gctctatgaa tttgttggag gaagcaacca aggggaagct tatctttgag
     3901 ggagtcgata ttacggacaa gaagaatgac ctgtttgcca tgcgtgagaa gatgggcatg
     3961 gttttccaac aatttaacct ctttcctaat atgactgtga tggaaaatat taccttgtca
     4021 cctatcaaga ctaaaggtga aagcagggaa gttgctgaga agagagccca agagcttttg
     4081 gaaaaagttg gtttgccaga taaggcagac gcttatccac agagtctgtc gggtgggcaa
     4141 caacaacgga ttgccatcgc gcgtgggttg gctatggaac cagatgtttt gctctttgac
     4201 gaaccaactt cagccttgga tcctgaaatg gtaggtgagg tattggctgt tatgcaagac
     4261 cttgccaagt cagggatgac tatggttatc gtaacacatg agatgggatt tgcccgtgag
     4321 gtggcagatc gtgttatctt tatggcagac ggtgtggttg ttgaagatgg gacacctgag
     4381 cagatttttg aacaaaccca agaacaacgg actaaagatt tcttgagtaa ggttttataa
     4441 tctattttaa aatttcaaga caagattagt gaaaagttca accagagctt tttcttatag
     4501 tttgaaacta taggataacc taggaaagaa gtgttagagg gcactagcag tttttggtat
     4561 aatggaagat atttgaaaga aaagagaagt gatatgacac agattattga tgggaaagct
     4621 ttagcggcca aattgcaggg gcagttggct gaaaagactg caaaattaaa ggaagaaaca
     4681 ggtctagtgc ctggtttggt agtgattttg gttggggata atccagccag ccaagtctac
     4741 gttcgcaaca aggagaggtc agctcttgcg gctggttccc gtagcgaagt agtgcgagtt
     4801 ccagagacca ttactcaaga ggaattgtta gacctgattg ctaaatataa tcaggatcca
     4861 gcttggcatg ggattttggt ccagttgcca ttaccaaaac atatcgatga agaggcggtt
     4921 ttattagcca ttgatccaga aaaggatgtg gatggtttcc atcccctaaa catgggacgt
     4981 ctttggtctg gacatccagt catgattccc tcgacacctg caggaattat ggaaatgttt
     5041 catgaatatg ggattgactt ggaaggtaaa aatgcggtcg tcatcggtcg ttcaaatatc
     5101 gttggaaaac ctatggctca gcttcttttg gctaaaaatg cgacagtaac cttgacccac
     5161 tcacgaactc ataatcttgc caaggtggct gctaaagcag atattcttgt agtcgcaatc
     5221 ggccgcgcca agtttgtgac tgctgacttt gtcaaaccag gtgcggttgt cattgacgtt
     5281 gggatgaatc gagatgaaaa tgggaagctc tgtggagatg ttgattatga ggcagttgca
     5341 ccacttgcta gccatatcac gccagtccct ggaggtgtcg gtcctatgac cattaccatg
     5401 ctgatggagc aaacttatca agcagcactt cggacattgg atagaaaata agagaaacat
     5461 tcgattaata gagtgtattt tctatagcta tatctaaaat ggcagaaatg aatattaaat
     5521 tttagatata agattacaaa aggaggtctg cgtctccttt ttattgtata ataaaagtga
     5581 gaggaaaaaa ggatgaaagt gattaatcaa accttattag aaaaagttat tattgaacgt
     5641 tctcgttcaa gtcataaagg agattatggt cgtctgctgc tgcttggtgg tacttatcct
     5701 tatggaggtg ccatcatcat ggctgcttta gcagctgtaa aaagcggtgc aggattggtg
     5761 accgttggaa cggacaggga aaatatcccg gctctgcaca gccatttacc tgagcctatg
     5821 gccttttctc ttcaagacca gcaattgtta aaagagcaat tggagaaggc agaagttgtc
     5881 ttgctggggc ctggtttacg agacgatgct tctggagaaa atctagtaaa acaggtcttt
     5941 gttaatttaa gccaaaatca gattttgatt gtagacggag gtgccttgac cattcttgct
     6001 aggacaagtt tgtcatttcc ctcaagccag cttatcctaa ctcctcacca aaaagaatgg
     6061 gaaaaattat ctggaattac gattgaaaag caaaaggaag atgcaacggc tagtgttctg
     6121 acttccttcc ctcaaggaac aattttggtg gagaaaggcc cagctactcg tatttgggaa
     6181 gttggccaat ctgattatta ccagttacag gttggtggtc cttatcaggc gactggggga
     6241 atgggagcta ctctggctgg gatgatcgca ggatttgtag gccaattccg acaggccagt
     6301 ctctacgagc gtgtggcagt agcgacccat cttcattcag ccatagccca agaactatct
     6361 caagaaaatt atgtggtctt accgacggaa atcagcaggt atcttcccaa aataatgaaa
     6421 attatttgtc aacaagagag ggtaagcaaa gataaacttg tttaaaatag acagataaat
     6481 ctacttcctt ctcttaattt atgattcttg aaatgcgcct gctttaaggc taaactagac
     6541 gtatcaaagt taagagtttg tttttgtaac tggaaagaga tgaacaaatc aaatacataa
     6601 aagataaact catttttatt cgtttggtaa aaataaaatg catattttta aagaaagatt
     6661 gatgaaaaat gtagatttga tgtttttact tgaataaaaa gcggtttcat agtaaaataa
     6721 gaactgagaa tgatggaggt aaatcggtgg aaaatctgaa gaaaatggca ggtatcaagg
     6781 ctgctgagtt cgtgagcgat ggaatggtcg ttggacttgg aacaggctcg actgcctatt
     6841 attttgtcga agaaatcggt cgtcgtatca aggaagaagg attgcagatt acagctgtaa
     6901 caacttctag tgtgaccact aaacaggcag aaggccttaa tatcccgctc aagtctattg
     6961 accaagtaga ttttgtcgat gtgacggttg atggcgcgga tgaagtggat agtcagttta
     7021 atggaatcaa aggcggtggt ggtgcccttc tcatggaaaa ggtggtcgca acaccatcaa
     7081 aagaatacat ttgggtggtg gatgaaagca agctggtcga aaaactaggt gcttttaaat
     7141 tgccagtaga agtggttcag tatggtgcag agcaggtctt tcgtcatttt gaacgagctg
     7201 gctacaaacc aagtttccgt gaaaaagacg gccaacgttt tgtgacggat atgcagaatt
     7261 ttatcattga cctcgccttg gatgtcattg aaaatccaat tgcttttgga caagaattgg
     7321 accatgtcgt tggtgttgtg gagcatggtt tattcaacca aatggtggat aaggtaatcg
     7381 ttgctggacg agatggagtt cagatttcaa cttcaaaaaa aggaaaatag aagggggcat
     7441 aagatgtcta aatttaatcg tattcatttg gtggtactgg attctgtagg aatcggtgca
     7501 gcaccagatg ctaataactt tgtcaatgca ggggttccag atggagcttc tgacacactg
     7561 ggacacattt caaaaacagt tggtttgaat gtcccaaaca tggctaaaat aggtcttgga
     7621 aatattcctc gtgaaactcc tcttaagact gtagcagctg aaagcaatcc aactggatat
     7681 gcaacaaaat tagaggaagt atctcttggt aaggatacta tgactggaca ctgggaaatc
     7741 atgggactca acattactga gcctttcgat actttctgga acggattccc agaagaaatc
     7801 ctgacaaaaa tcgaagaatt ctcaggacgc aaggttattc gtgaagccaa caaaccttat
     7861 tcaggaacgg ctgttatcta tgattttgga ccacgtcaga tggaaactgg agagttgatt
     7921 atctatactt cagctgaccc tgttttgcag attgctgccc acgaagacat tattcctttg
     7981 gatgaattgt accgtatctg tgaatacgct cgttcgatta cccttgagcg tcctgccctt
     8041 cttggtcgca tcattgctcg cccttatgta ggtgaaccag gtaacttcac tcgtacggca
     8101 aaccgtcgtg acttggctgt atctccattt tccccaactg ttttggataa attgaatgag
     8161 gctggtatcg atacttatgc tgtgggtaaa atcaacgata tctttaacgg tgctggtatc
     8221 aaccatgaca tgggtcacaa caagtcaaat agtcatggaa ttgatacact attgaagact
     8281 atgggacttg ctgagtttga aaaaggattc tcattcacaa acctagttga ctttgatgcc
     8341 ctttacggcc atcgtcgtaa tgctcacggt taccgtgatt gcttgcatga gtttgatgaa
     8401 cgcttacctg aaattatcgc agctatgaga gagaatgacc ttctcttgat tactgcggac
     8461 catggaaatg acccaacgta tgcaggaacg gatcacactc gggaatatat tccattgttg
     8521 gcctatagcc ctgcctttaa aggaaatggt ctcattccag taggacattt tgcagatatt
     8581 tcagcgactg ttgccgataa ctttggtgtg gaaactgcta tgattgggga aagtttctta
     8641 gataaattgg tataagatga cgcgctatgc tttgctggtg agaggtatca atgttggtgg
     8701 taagaataag gtcgtcatgg cggagcttcg tcaagaattg acaaacttgg gactggaaaa
     8761 ggttgagagc tacatcaata gtggcaatat tttctttact tcgatagatt ccaaagccca
     8821 attggttgaa aagctagaga ctttctttgc agtccattat ccatttattc agagcttttc
     8881 tttactgagt ctagaggact ttgaggcgga acttgaaaat ctaccagctt ggtggagcag
     8941 agacttggca cgaaaagatt ttctctttta cactgagggt ttggatgtgg accaagtcat
     9001 cgcgacagtt gaaagtttag agctgaaaga tgaagtgctt tattttggaa aacttgggat
     9061 tttctggggg aaattttctg aagaatccta ttctaagact gcctatcata agtacttgct
     9121 gaaggtgcct ttctaccgcc acattactat tcgtaatgct aaaacctttg acaaaattgg
     9181 tcaaatgcta aaaaaataat aaaggagaca cacaatgaca tttttaaaca aaatccatga
     9241 aactgctact ttcctgaaag aaaagggaat tgcagcccct gagttcggtc taatccttgg
     9301 atcaggactt ggagaattgg cagaagaaat cgaaaatcca gttgtagtag actatgctga
     9361 gatttcaaac tggggccgtt caacagtagt cggtcatgct ggtaaattgg tatatggtga
     9421 actggcaggt cgcaaggtct tggctcttca agggcgtttc catttctatg aagggaatcc
     9481 tctggaagtg gtgactttcc cagttcgtgt gatgaaagtt cttggatgtg aaggtgttat
     9541 tgtaaccaat gcagctggcg gtatcggatt tggtcctggt accttgatgg ctatctcaga
     9601 ccatatcaac atgacggggc aaaatccatt gatgggtgaa aacttggatg actttggccc
     9661 acgtttccca gatatgtcta gggcctacac accagaatac cgtgccactg cccatgaagt
     9721 ggctaaaaaa cttaatatca agcttgatga aggtgtctat atcggagtta ctggtccgac
     9781 ttatgaaaca ccagcagaaa ttcgttccta taagacactg ggagcagatg cagttggtat
     9841 gtctacggtt cctgaagtta tcgtggcagc ccactctggc ttgaaagttc tgggaatttc
     9901 atgtatcact aactttgcgg ccggtttcca agaagaactc aatcacgaag aagttgtaga
     9961 agtgactgaa cgtgttaaag gtgatttcaa aggcttgctt aaagcgattc ttgctgaatt
    10021 gtaagaaaaa agatttaaaa gggggagtgc ctctgttttt tcaggattga ctgcctatcc
    10081 ggattaaaga agaaacagag gaatactatg agcttcttcc tgctcttata actgaaagaa
    10141 gcggaagaat aggtatgtct gatctgatag ccagcattgt gaaagacaag attctaggat
    10201 actagcatta gcttcctagc caagcagact agtatgataa ggagagatga gaatgaattg
    10261 actttctgaa tttctcagtc ttatcatata tagcacaatg agatttcgct tgagtctgct
    10321 tgtaaataaa cgaaaagaaa gataagaaat aatgaaaatt ggtcaacgaa ttatgcgctt
    10381 tggcataaaa aattaagtat cggagttgta tctgttgtag tcggctttga tttctagctc
    10441 cagctggaat ttcagccaat gaagtaaagc aagatgtaac atctgaagtg gtaataggtg
    10501 tgctagattc taaggaggaa ttgaaagagt cagaaaatga tgctccaaaa ctagaaactc
    10561 ctcttagaga ggagccaaga ctagctcctc aaacgcttcc ggaagcaagt gaagttcttg
    10621 aaaacaaaag ggaagagtca aaagtagaga taacataacc agctcaagcg gatgatatcc
    10681 gcaaggttgt tggggaatta gccaaggata taagtattac taagttgtat atgacaggtc
    10741 attctcttgg atgttaccta gctcagattg cagcggttga agcttaccaa aaatatcctg
    10801 atttttataa ccatgtattg aggaaagtga caactttcag tgctcctaaa gtgattactt
    10861 ccagaactgt ttggaatgct aagaatggtt tctgggatgt tggtttggaa agtcgtaaat
    10921 tagctgttag tggaaaaatt aagcattatg tggttgataa tgacaatgtt gtgactccct
    10981 tgattcataa taatcgtgat attgttacat ttacaggtaa ttcacgcttt aaacaccgtt
    11041 ctcgtggcta ttttgaaagt ccaatgaatg atattcctaa ctttaatatt ggtaaacaag
    11101 ctaccttgga taaacatggt tatcgtgatc cgaaattgga taaagtgcga ttctttaaga
    11161 aacaggctct gcctcgatct tctagtcaac caagcgctga accaatggaa aatattgcct
    11221 caggaaaaca ggttactcaa agttcgacag ctttcggagg agatgctaga agagctgtgg
    11281 atggcaaagt cgatggtaac tatggtcaca attctgtcac tcatacaaac ttccaatcta
    11341 agccttggtg gcaagtagat ttggtaaaga agaaaccatt cgccaaatca atatttacaa
    11401 ccgaacagac actgcccagg atagattggc aaactttgat gtcattcttt tagacagttc
    11461 tggtaaagaa attgagtgaa aacgtataac atctcctaaa gatgtgtcag cacaaattac
    11521 gattaaccat aaaaaagcgc gctatgttcg gattgagcta gaaggctata atgccctcag
    11581 tcttgcagaa gttgaagttt tctgctttat agctacgaat gctgaaacgg cgacacaagt
    11641 ttctaagcca gttcaaccaa tcagtcagac tcctgtgaag gataaaacat tgacaattca
    11701 acacagtgga gcttacattg cccgctactc cataacttgg gaagaagttc cagtagataa
    11761 agatggaaac caagttgttc gtagtcattc ttgggaagga agcggtcgca accagactgc
    11821 aggttttgtc ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga
    11881 gaaaaagacg ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt
    11941 agctcaaccc caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga
    12001 cgatgatgtc ttgtaatctg atggtagaat gacagttagt ttgtctagtt tataagaaag
    12061 tactacctga gcttgaatag gactcaggta gctctctatg aaagaacaaa attaatactc
    12121 aatgaaaatc aaagagcaaa ctaagaaact agccgcaggt tgctcaaagc actgctttga
    12181 ggttgtagat aagactgacg aagtcagtca catatataat ccaaggcgac gttgacgtgg
    12241 tttgaagaga ttttcgaaga gtataaacag aaaggtagag cgcgtgttct aatttgaaca
    12301 cgagtagaaa acttttctaa aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac
    12361 tgaatacggg cgactctcct ctaaatcaaa attaagaaag gaattgaccc caccctaaaa
    12421 gtagtgggaa aaagatagtt gatctagcga gcatcgctca ctgcgcccaa ctcctatttt
    12481 cccttcgctt tttgatgggt ttggtatctt tctcaatata aaatataaaa taaagaaagg
    12541 tagagcgtgt gttttgattt gaacacgagc ggaaaactcg gaaaatagat aatctgactg
    12601 aaaaatcagg atttctcgtc aggttcctaa ttttcagtcg ttttcttctc gctctttgta
    12661 tcataaatta tgtctatcca tattgctgct cagcagggtg aaattgctga taaaattctt
    12721 cttcctgggg atcctcttcg tgctaagttt attgcggaga atttccttga tgatgctgtt
    12781 tgttttaacg aagtgcgtaa catgtttggt tacactggta cttacaaggg tcactgtgta
    12841 tctgtcatgg gaactgggat gggaatgcca tctatttcga tttatgcgcg tgagttaatc
    12901 gtagactacg gtgtgaagaa attgattcgt gtgggaactg caggttcttt gaatgaagag
    12961 gttcatgttc gtgaattagt tttggcgcag gcggctgcaa ccaactcaaa catcgttcgt
    13021 aatgactggc cacagtacga ttttccacaa attgctagct ttgatttgct tgataaagcc
    13081 taccatatcg ccaaaaaact tggtatgact actcacgttg ggaacgtttt gtcatctgat
    13141 gtcttttact caaattactt tgaaaagaat atcgagcttg gtaaatgggg agtcaaggct
    13201 gtggaaatgg aagcagcagc tctttactat cttgctgccc aataccatgt tgatgcgcta
    13261 gctatcatga ccatctctga tagcttggtc aatccagacg aagacacaac tgcagaagaa
    13321 cgtcaaaata ccttcactga tatgatgaag gttggtttgg aaaccttgat tgcagaataa
    13381 ttatagccaa aaaggggctc tttgtcaact gtagtgggtt gaaaaaaagc taagcttgag
    13441 aaaggacaaa tttcgtcctt tcttttttga tattcagggc gataaaaatc cgttttttga
    13501 agttttcaaa gttccgaaaa ccaaaggcat tgcgcttgat aagtttgatg agattattgg
    13561 tcgcttccag tttggcatta gaatag
//
LOCUS       DAVKHZ010000058        13182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000058 DAVKHZ010000000
VERSION     DAVKHZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.058"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            82..1341
                     /locus_tag="VWW99_001568"
                     /pseudo
     CDS             82..1341
                     /locus_tag="VWW99_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            1437..2711
                     /locus_tag="VWW99_001569"
     CDS             1437..2711
                     /locus_tag="VWW99_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9539384.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2721..3599
                     /gene="truB"
                     /locus_tag="VWW99_001570"
     CDS             2721..3599
                     /gene="truB"
                     /locus_tag="VWW99_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9539385.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEDATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3841..4143
                     /locus_tag="VWW99_001571"
     CDS             3841..4143
                     /locus_tag="VWW99_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9539386.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKHQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFSLWIAKAPSQERGRLAFLQMLAQILQGFR"
     gene            4251..4889
                     /gene="udk"
                     /locus_tag="VWW99_001572"
     CDS             4251..4889
                     /gene="udk"
                     /locus_tag="VWW99_001572"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9539387.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5017..6357
                     /locus_tag="VWW99_001573"
     CDS             5017..6357
                     /locus_tag="VWW99_001573"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9539388.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            6335..6547
                     /locus_tag="VWW99_001574"
     CDS             6335..6547
                     /locus_tag="VWW99_001574"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9539389.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6544..7419
                     /locus_tag="VWW99_001575"
     CDS             6544..7419
                     /locus_tag="VWW99_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9539390.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            7412..8227
                     /locus_tag="VWW99_001576"
     CDS             7412..8227
                     /locus_tag="VWW99_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9539391.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            8220..8651
                     /locus_tag="VWW99_001577"
     CDS             8220..8651
                     /locus_tag="VWW99_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9539392.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8658..10325
                     /gene="recN"
                     /locus_tag="VWW99_001578"
     CDS             8658..10325
                     /gene="recN"
                     /locus_tag="VWW99_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9539393.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10327..11055
                     /locus_tag="VWW99_001579"
     CDS             10327..11055
                     /locus_tag="VWW99_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9539394.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11134..12957
                     /gene="lepA"
                     /locus_tag="VWW99_001580"
     CDS             11134..12957
                     /gene="lepA"
                     /locus_tag="VWW99_001580"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9539395.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13038..>13182)
                     /locus_tag="VWW99_001581"
     CDS             complement(13038..>13182)
                     /locus_tag="VWW99_001581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539396.1"
                     /translation="KEQTRKLAAGYSKHSFEVVDETDEVSNHTYSKATLTWFEEIFEE
                     YKN"
BASE COUNT         3915 a         2430 c         3164 g         3673 t
ORIGIN      
        1 caaaatatag aaaactgata tttgtaaact ataactcaag gtgctacaat atccttaata
       61 aaatgatatg gaggtcacct tatgacttgt gattttaaat ctgaaactct acaactacat
      121 gctggtcaag ttgtggctcc agctactaag tctcgtgcag tgccgattta tcaaacaaca
      181 ttttttgttt ttgatgacac gtaggaaggt gccgatctgt ttgccttgag gaaaccaggg
      241 aacatttata ctcgtatcac caatcctaca acagctgccc ttgaaggtgg tgttgaagcg
      301 ctagcaacag catcaggtat gactgcagtg acttatacga ttttggcgct tgcccatgct
      361 ggtgaccatg tagtggctgc ttcgactatt tacggtggaa ccttcaatct tttgaaagaa
      421 ccccttcctc gttatggtat cacaacaacc tttgtcgatg ttgataattt ggaggaagta
      481 gaagcagcta tcaaagacaa taccaagctt gtcttgattg aaaccttggg taaccccttg
      541 attaatattc cagacctgga aaaactggca gagattgctc ataaacatca aatcccactt
      601 gtgtcagaca atacttttgc aataccttat ttgattaacg tcttctctca tggcgttgac
      661 attgccattc actctgcgac taagtttatc ggtgggcatg gtacaactat tggaggaata
      721 attgtcgata gtggtcgttt tgactggacg gcttcaggga aattccctca atttgttgac
      781 gagggttcaa gctgccacaa tttgagctat attcgtgatg tgggtgcagc aacctttatt
      841 atagctgttc gagttcaatt gcttcgtgat acaggtgcag tcttgtcacc attcaatgct
      901 ttcctcttgc tacaaagact tgaaactctt tcacttcgtg tggaacgcca tgtacaaaat
      961 gctgagacaa ttgttgattt tcttgtcaac catcctaagg tagaaaaggt aaattatcca
     1021 aaacttgcag atagtcctta tcatgccttg gctgagaaat acttgccaaa aggtgtcggt
     1081 tcaatcttta ccttccacgt caaaggtgac gaggaagaag cacgcaaggt cattgatcat
     1141 ttagaaatct tttctgacct tgcaaacgcg gcagatgcta aatcgcttgt tgtccatcca
     1201 gcaacaacca ctcacggtca attgtcagaa aaagacctag aagcagcagg tgtcacacca
     1261 aaccaaattc gtctgtcaat cggtcttgaa aatgtagaag atttgattga agacttacgc
     1321 ttggccttgg aaaaaattta aagtaaaaga agataaacag tgggttttga ctcactgttt
     1381 ttgattttcc ctcaggcatg atataatggt tacagaagtc tagaaagagg aatgtaatga
     1441 acgaaatcaa atgtcccaac tgtggggaag tctttacagt aaatgaaagc cagtatgctg
     1501 aacttttgtc ccaagtgaga acggcagagt ttgataagga actacacgat aggatgaggc
     1561 aggaactggc cttggctgag caaaaggcta tgaatgagca acagactaaa ctggctcaga
     1621 aggatcaaga aattgcgcaa ttgcagagtc aaatccaaaa ctttgataca gaaaaagaat
     1681 tggccaagaa agaagttgaa cagacaagcc atcaggcctt attggctaag gacaaggaag
     1741 tacaggcttt ggagaaccaa ttggccactt tgcgtttgga gcatgaaaat caactgcaaa
     1801 agaccctttc tgacctagaa aaagaacgca atcaggtcaa aaatcagctc ctactgcaag
     1861 aaaaggaaaa tgagttgtct ttggcttctg ttaagcaaaa ctacgaagcc cagctcaaag
     1921 cagccagtga acaagtcgag ttttataaga attttaaggc tcaacaatct acaaaagcga
     1981 ttggggaaag cctagaacag tatgcagaga gtgagtttaa caaggtccgt agtttcgcct
     2041 ttccaaatgc ttactttgag aaggataaca aggtctcttc gcgtgggtct aaaggggact
     2101 ttatcttccg tgagtgtgat gaaaatggag ttgaaatcat ttctatcatg tttgagatga
     2161 aaaacgaagc ggacggaaca gagaagaagc acaagaatgc agatttttac aaggaattgg
     2221 acaaggaccg tcgggagaag aactgtgagt atgccgtttt ggtgaccatg cttgaggccg
     2281 ataatgacta ctttaacaca gggattgttg acgtcagcca cgagtatgaa aaaatgtatg
     2341 ttgttcgtcc tcaattcttt atccagttga ttggtctctt acgtaatgcg gcgctaaatt
     2401 ccctaaaata caagcaggag ttggccttgg ttcgcgagca aaatattgac attacgcatt
     2461 ttgaggaaga tttggatgcc tttaagctag cttttgctaa gaactataat tcagcttcga
     2521 ctaactttgg aaaagctatt gatgaaatcg acaaggccat caaacgcatg gaagaggtta
     2581 agaaattcct gactacatct gaaaaccaac tccgtctagc taacaacaaa ttggaagatg
     2641 tctctgttaa aaaattgacc cggaaaaatc caacaatgaa agcgaagttc gaagcattga
     2701 agggggagta gaaagcaaaa atgaacggta ttatcaactt aaaaaaagaa gcaggaatga
     2761 cctcgcatga tgcggttttt aaactgcgta agattttggg aaccaagaaa attggtcatg
     2821 gcggaacctt ggatccggat gtggtgggtg ttttgccgat tgcggttggc aaggcgacac
     2881 gcatggtcga gtttatgcag gacgagggta agatctatga gggggaaatc actctgggct
     2941 attccacgac gactgaggat gctagtgggg aagtggtcgc agaaacccct gttttgtctc
     3001 ccttggatga aaagcttgtt gatgaagcga ttgctagctt gactgggcct attacccaga
     3061 ttccccctat gtattcggca gttaaggtta atggtcgcaa gctctatgag tatgcgcgtg
     3121 ctggtcagga agtggagcgt ccagaacgtc aggtgaccat ttatcaattt gagcgaacaa
     3181 gtccgatttc ttatgatggc caacttgccc gattcacttt tcgtgtaaaa tgcagtaaag
     3241 ggacttacat ccgtactttg tcagttgatt tgggtgaaaa gcttggttat gcggctcata
     3301 tgtcccattt gactcgtact agtgctgctg gcttacaatt agaagacgct cttgccttgg
     3361 aggaaattgc tgaaaaagta gaggctgggc aattagattt tctccatcct ttagagattg
     3421 ggacaggtga ccttgtcaaa gttttcctaa ctccagaaga tgctacagaa gttcgctttg
     3481 gtcgttttat tgagctagac caaacggaca aagaactggc tgcctttgaa gatgataaat
     3541 tgttagccat tctagaaaaa cggggcaatc tctataagcc aaggaaggtt tttagctaga
     3601 tcgtttagga ataaaaatcg ggtgatagat aacaattgct tgataaaacc ccatactaaa
     3661 tagtagaatg gttttgggaa ttataatatt ccaattgttg cgagttgtag gtactcaaat
     3721 aatctatata gaaatttaga ggtgtgaaat gaagcaattt aaaattcttt cagataaata
     3781 tttagagtcc attacaggtt ctggtgggaa cttaggccca ggatttggtg tgataattcc
     3841 atgatgcgaa atgagtttcg agaaagggtg gagcaacttc ttcaacaaaa agaaataaat
     3901 gaaaatagtg agttgagtca cctgtttcgt cttgctatac aaaatttaga cagaaatgaa
     3961 aaacaccaat cggtcatggc caatttgagt caagggttgt cactttacct catgacgcat
     4021 cattaccagg cacctaagtc tgtcattgat tttagtttat ggattgccaa agctcctagc
     4081 caggaaagag ggaggctggc tttcttgcag atgcttgctc aaatcctaca gggctttcgt
     4141 taaaactgtc gaacatcaga gggaagactc tggtgttttt tgctgatgag actataaatt
     4201 ttgtaaaaaa tgtgatagaa tagacgacgg ataaaataac ggaggatagc atgcaaaata
     4261 gaccaatcat tattggagtg acaggtggtt ctggtggtgg taaaaccagt gtttcaagag
     4321 ccattttatc gcattttcct gatgaaaaga tttccatgat tgagcatgat tcatactaca
     4381 aggatcagtc tcatttgacc tttgaagagc gtgtcaaaac caactatgac catccttttg
     4441 cctttgatac agacttgatg atcgagcaga ttaaggaatt gttggcaggg cgtccggtgg
     4501 acatcccgac ctacgactat acagagcata cacggagtag caagacctat cgtcaggagc
     4561 ctcaagatgt ctttatcgtt gagggcattt tggtcttgga ggacaagcgt ctgcgcgatt
     4621 tgatggatat caagattttt gtggatacgg atgacgatgt gcgcattatt cgtcgtatca
     4681 agcgtgatat ggaggagcgt ggccgtagcc ttgatagcgt tattaaccag tacttaggtg
     4741 tggtcaaacc aatgtaccac cagtttatcg agtcaactaa gcgttatgct gatatcgtca
     4801 ttcctgaagg ggttagcaat accgtggcta tcgacctgtt gacgaccaag attgcaaaga
     4861 ttttggaaga agctcgaaac agcaaataat cacatgtgga ggccttgcct cctttttcta
     4921 tttttccctc ataatggtac ataaatggag atttttaggc atatttttgg tataataata
     4981 cccatgaaag agcaagtaaa tgaatagtag gtggagatgg aaaagtattt atcggtaaca
     5041 actttgacca agtatctgaa aatgaaattc gataaagacc catacttgga acgggtctat
     5101 ttaactggtc aagtttccaa ctttcgtaaa cgacctactc accaatattt ctccctaaag
     5161 gatgaccatg cagttattca agcgaccatc tggtctggga tttatcagaa attagggttt
     5221 gacctggaag aaggaatgaa gatcaatgtg attgggcgtg tacaggttta tgaaccaagt
     5281 ggtagctact ccatcatcat tgaaaaagct gagcctgatg gggttggggc gcttgcgatt
     5341 cagtttgaac aacttaagaa aaaattgaca gaagaaggcc tgtttcaaga acgcttcaag
     5401 caagctctac cccaattttc taagagaatt ggggtagtaa ccagccgtag tggagccgtt
     5461 attcgagata ttatcacgac cgtcagcagg cgattcccag gtgttgacat ccttctttat
     5521 ccgaccaagg ttcaaggtga aggggctgcg gaggaaattg ctagaaatat tgcgcgtgct
     5581 aatcaacggg atgatttgga tttgctcatt attggtcgtg gaggtggttc tattgaggat
     5641 ctctgggcct ttaacgaaga aattgtggta cgagctattt ttgaatctcg tttgccagtt
     5701 atctctagtg tggggcatga gacggatgtg accttggcag attttgtggc agatcgacgc
     5761 gctgcaacgc caacagcggc ggctgaactg gcaacacctg tgaccaagtt ggatgtatta
     5821 gctcatttgc aaaatcagga aaaacggatg gcaacggcag tccgaaatgt tctatctaag
     5881 aaacaagagg ctttgaaaaa atgcagtcag tctgttatct ttagacaacc tgagcgcttg
     5941 tatgacggtt atttgcaacg cttggaccaa ctgcaactgc gtttgaaaca aagtttgcga
     6001 actcggattt ctgataacaa acaattagtt caagcaagaa ctcatcaatt agtacaatta
     6061 tcacctgtta ccaaaatcca acgctatcaa gaccgtttag gacagttgga caagctctta
     6121 ggtagccaaa tggcgttagt ttatgacgcc aaggttgctg aggccaagcg actttcggaa
     6181 gctttgctca tgttggatac tagccgaatc gtggcgcgtg gttatgctat tgtcaaaaaa
     6241 gaagagtcca ttgtagattc ggttgagagt ttgaagaaaa aagaccaagt aacgcttttg
     6301 atgcgagatg gtcaagtaga attagaggtt aaagatgtca aaacaaaaga aatttgagga
     6361 aaatctagca gaactggaaa ccattgtcca aagtttggaa aatggtgaaa ttgctctgga
     6421 agatgcgatt actgcctttc aaaagggcat ggtcttgtca aaagagctcc aagctacgct
     6481 ggacaaggct gaaaagacct tggtcaaggt catgcaagaa gacggaacag aaagtgattt
     6541 tgaatgaaaa agcaagaaaa attagctctt gtcgagtcgg ctttggaaga tttttatgga
     6601 gaccagcagt ttgcctctag tttacgggag tctgttctct attctattca tgctggtggc
     6661 aagcgtattc ggccttttct cttgttagaa gttctggaat ccttgcaggt tgccatcaaa
     6721 cctgctcacg cgcaggtagc tactgccttg gagatgattc atacagggag cttgattcac
     6781 gatgaccttc ctgctatgga tgatgacgat tatcgaagag ggcggttaac caatcacaag
     6841 aaattcggtg aagctatggc cattttggct ggagatgcct tattcttaga cccatatgcc
     6901 ttgattgcgc aggcagattt gccaagtcag atcaaggtgg acttgattgc caacttatcc
     6961 cttgcttcag gtagtctggg tatggtggca gggcaagttt tggatatgga gggcgaacac
     7021 cagcacttgt ctctggaaga acttcagact attcatgcca ataagactgg gaagttacta
     7081 gcctatccct tccaagcggc agctattata gctgaattgt cacctgaaat gcaggtgaag
     7141 ctgaaaactg tgggtgaatt gattggactt gcttttcaag tcagagatga tgtactggat
     7201 gtgacagcta gttttgagga aatcggcaag acacctcaaa aggatctgca ggcagaaaaa
     7261 tcaacctatc ctgccttgtt gggcttggaa gagtccattg ccttttgtaa ccaaaccctg
     7321 gatcaagcta atgaaaaatt agaagagatt gcccagcagc ttccctttga aacagaatcg
     7381 attgtaagtg tagtagaaag tttgagaatc aatggctaag gaaagagtgg atgtactagc
     7441 ttataaacag gggttgtttg aaacgagaga gcaggccaag cgaggtgtga tggctggtct
     7501 agtcgtagca gtccttaatg gagaacggtt tgacaagcca ggagagaaaa ttccagatga
     7561 caccgaatta aaactcaagg gggagaaact caagtatgtc agccgtggtg gtttgaaact
     7621 ggaaaaggcc ttgcaggtct ttgatttgtc ggtggatggc gcgactacga ttgatatcgg
     7681 ggcctctact ggaggtttta ccgatgtcat gctacagaat agtgccaagt tggtctttgc
     7741 agtcgatgtt ggtaccaatc agttggcttg gaaattacgc caagacccac gagttgtcag
     7801 catggagcag ttcaattttc gctatgctga aaagactgat ttcgagcagg agccgagctt
     7861 tgccagtatt gatgtgagtt tcatttccct tagtctgatt ttgccagcct tgcaccgtgt
     7921 cttggctgat caaggtcagg tggtagcact tgtcaaacct cagtttgagg caggacgtga
     7981 gcagattggg aaaaatggaa ttattcgaga tgctaagatt catcagaatg tccttgaatc
     8041 tgtaacagct atggcagtag aggcaggttt ttcagtcctt ggcttggact tttctcccat
     8101 ccaaggtgga catggaaata ttgaattttt agcgtatttg aaaaaagaaa agtcagcaag
     8161 caatcagatt cttgctgaga ttaaagaagc agtagagagg gcgcatagtc aatttaaaaa
     8221 tgaataaaaa agaaagactt gaaaaaatta gaagattggt gacagattat caaatcggca
     8281 cgcaagaaga aattgtagaa catttgaaag aagcaggtat cactgccact caggcgacgg
     8341 tatcccgaga tatcaaagag ttaggtattg tcaaaattcc tttgagagac aacacctatg
     8401 tctacgaatt gccaaaatca atcgtaaaaa gtctgcaact ggctgaagac aatatcgaat
     8461 cggctgaatt gatggataag atgatcaatc tccaagttat tccaggaaat acggcttttg
     8521 taaaagctca gttaatcgag acttttgcag acaagatttt tagctgtttg actgatgata
     8581 gctcgatttt agtcattgcc agaagtggaa gtctagcaga ggaaatcttt gaacaagtaa
     8641 aaaattggta ggtctatatg ttacttgaaa tttcgataaa aaactttgcc attattgagg
     8701 ctatttccct caattttgaa aaggggatga ctgtcctgac tggtgaaacg ggtgcaggga
     8761 agtcgattat cattgatgcc atgaatatga tgttgggagc tcgtgcgacg acagatgtta
     8821 ttcgtcatgg tgcaccaaag gcagagattg aggggctttt ctcagttgag aatagtcgtc
     8881 ttttacagga aatttttgat gagcaaggtt tagaattagg tgatgaaatt atcatccgtc
     8941 gggaaatctt gcaaaatggt cgtagtatca gccgtgtaaa tggtcagatg gttaatctgt
     9001 ctgttttgcg aaccattggg caacatcttg tagatattca tggtcagcat gaccatgagg
     9061 agttaatgcg tccccaactg catatccaga tgttggatga atttggtgat gccgcttttt
     9121 gggacttgaa agaaacctat caaacgagtt ttgatgccta tcggaaaatg cgcaagcagg
     9181 ttctggaagt caagaaaaac caacaggaac acaaggcacg tatcgaaatg ttggaatttc
     9241 aaatggcaga gattgaggca gcaaacttgc aggctggaga agacttggat ctcaatcaag
     9301 agcgagataa actcctcaac cacaaaaata ttgcggatac actgaccaat gcctacagta
     9361 tgttggacaa tgaagatttt tcaagtctgg ccaatgttcg ttcagccatg aatgacatgg
     9421 aaagtgtcga agagtatgac cctgaatacc gtgaaatttc aagctctctg tctgagacct
     9481 actatgtttt agaagacatt agcaaacgtt tggaagctat cattgaggac cttgattttg
     9541 atggcaatcg cctcatgcag gttgagaatc gtttggacct ccttcatact attactcgta
     9601 agtatggtgg gactgttgat gatgttttgc tttattttgc caagattacg gaagaataca
     9661 atctcttgac aggcaataat ctttcgtctg aggacatgga agcagagctt aagaagttgg
     9721 aagtcaatct tgtcaatttg gcaggtcaac ttgcttctgc tcgtcataat ttggctcagc
     9781 aactcgaagc tgagattaaa caagaactgc aagatcttta tatggaaaaa gcccagtttc
     9841 aggttcgttt tagtaaggga aaattcagtc gtgagggaaa tgaaatggtt gagttttata
     9901 tttcaaccaa ccctggagaa gactttaaac ccttggttaa ggttgcttct ggaggggaat
     9961 tatctcgtct catgttagcc attaagtctg ccttttcacg taaagaaggc aagactagca
    10021 ttgtctttga tgaagtggat acgggagttt caggtcgtgt tgctcaagct attgctcaga
    10081 aaattcataa aattggtcag catggtcagg ttttggctat ctcccatttg ccacaagtaa
    10141 ttgcgattgc agattatcaa ttctttattg agaagattag taatgaccat tcaacggttt
    10201 cgactgttcg tctcttgacg gtcgaagagc gagtggagga agttgccaag atgttggcag
    10261 gtgatgatgt gacagaagca gccctgacgc aagccagaga attgttgaga aacagggaga
    10321 aataagatga cagattatta tgtaattgga gatgttcacg gaaaagctgg gatgctggaa
    10381 gaccttctta aaacctggga tggtcagacc cagttgctct ttctaggtga tttgattgac
    10441 agaggtgagg atagtcaccg tgttcttgaa atggtcaagg acttggtgga caatcaaggg
    10501 gctatctgtt tgtctggaaa ccacgagtat atgtttttga cttggctaga tgacccagaa
    10561 gaaagttatg accattatcg tcgcaatggt ggagatacaa ccattaactc tatcctaggt
    10621 cgtcccttgg atgcaccagt tgatggagta gaagatgcca agcgggttgc cgctgaagcg
    10681 gcagacttgg tcgaattcat tcgtcaaatg ccatttgtag tagagacaga caagtatatc
    10741 tttgttcacg caggtattga tttgaccttg gatgactggc atgaaacaac agattataaa
    10801 aaagtatggc ttagaaaacc attccacgga gctgaaaatc atactggaaa aaccattgtc
    10861 ttcggtcata caccggtcta tggtttgtta cagcaagaac gcggtacatc tgagctttgg
    10921 actacagaag atggcaagat tggaatggat ggaggagctg tttatggtgg tgtccttcac
    10981 gggattgtct ttacagacca aggaatgaca gaacaccact ttatcgaaaa tgacggcttt
    11041 gtcgctgaag attagtactc ctagcagggt atggtcttgt caaaatatca aaaacaattt
    11101 ataataaata gataccctga aaggaagaga atcatgaact tagaagaatt gaaaaaacga
    11161 caggagaaga tccgtaactt ctctattatc gcccatattg accatggaaa atcaactcta
    11221 gcagaccgca ttttggaaaa aacagagacg gtttcaagtc gtgaaatgca ggcccagctt
    11281 ttggatagca tggagctaga gcgggaacgt ggaattacta ttaagttgaa tgccatcgag
    11341 ttgaattaca ctgcaaaaga tggggaaact tatattttcc atttgattga cacaccaggg
    11401 cacgttgact ttacctatga agtttcacgt tcgctagctg cctgtgaggg tgctattttg
    11461 gtggtcgatg cggctcaagg aattgaggct caaactcttg ccaacgttta tctggctttg
    11521 gataatgatt tggaaatcat gcctatcatt aataaaattg acctgccggc tgcagatccg
    11581 gagcgcgtgc gtacagagat tgaagatgtg attggtttgg atgccagtga agcagttttg
    11641 gcttctgcca aggctggtat tgggattgaa gaaatcctcg agcaaattgt agaaaaagta
    11701 ccagcaccaa cgggtgatgt gacggcgcca cttaaggcct tgattttcga ctctgtttac
    11761 gatgcttacc gtggggttat cctccaagtg cgtgtcatgg acggagtggt caaacctggt
    11821 gataagattc agctcatgag caatagtaag acctttgatg tggccgaagt cggtattttt
    11881 acaccaaaag cggttggtcg tgatttcctt gcgactggtg atgttggtta cattgcggcg
    11941 tctatcaaga cggttcagga tactcgtgtg ggtgataccg ttaccttggc aaccaatcct
    12001 gcggcagaac cattacatgg ttataagcag atgaatccta tggtctttgc gggtctctac
    12061 ccaatcgaat caaacaaata caatgacctg cgtgaagcgc ttgaaaaatt gcaactgaat
    12121 gatgctagtc ttcagtttga accagaaaca tctcaggcac ttggatttgg tttccgttgt
    12181 ggatttcttg gacttctcca tatggatgtt atccaggaac gtttagagcg tgagttcaat
    12241 attgacctca tcatgacagc tccgtctgtt atttacaaag ttaatttgac cgacggtgag
    12301 tctatggatg tgtctaaccc atctgagttt ccagacccaa ctaagattgc gaccattgaa
    12361 gagccttatg tcaaggcgca aatcatggta ccacaggagt tcgtcggagc agtaatggag
    12421 ctagctcagc gtaagcgtgg ggactttgtg actatggact atattgatga taaccgtgtc
    12481 aatgttatct atcaaattcc tcttgctgaa attgtctttg acttctttga taaacttaag
    12541 tcttcgacac gtggttatgc aagctttgac tacgaattgt cagaatatcg cccatctaag
    12601 ctggtgaaaa tggatatcct tctcaatgga gataaggtgg atgccctcag ctttatcgtt
    12661 cacaaggact ttgcctacga acgtgggaaa ctcatcgttg ataaactcaa gaaaatcatc
    12721 cctcgccaac aatttgaagt tccaatccaa gcggctattg gacacaagat tgtcgctcgt
    12781 actgatatca aggcccttcg taagaacgta cttgctaaat gttatggtgg tgacgtttct
    12841 cgtaagcgta aactccttga aaaacaaaaa gctggtaaga aacgcatgaa atccatcgga
    12901 tcagttgaag ttccgcaaga agccttcctc agcgtcttga gtatggatga agaataagtt
    12961 atcagaaatg cctttccttg ttcacaggaa atttatataa gaatcaatag taaagcaggc
    13021 ttagatggtc tgctttttta gtttttatac tcttcgaaaa tctcttcaaa ccacgtcagc
    13081 gtcgccttac tgtatgtatg gttactgact tcgtcagttt catctacaac ctcaaaactg
    13141 tgttttgagt aacctgcggc tagcttccta gtttgctctt tg
//
LOCUS       DAVKHZ010000059        12900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000059 DAVKHZ010000000
VERSION     DAVKHZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.059"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..1125
                     /gene="dnaN"
                     /locus_tag="VWW99_001582"
     CDS             <1..1125
                     /gene="dnaN"
                     /locus_tag="VWW99_001582"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9539397.1"
                     /translation="SINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGITLIGS
                     NGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVL
                     TSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTG
                     VHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETV
                     EIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARL
                     LSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSL
                     KALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1190..1384
                     /locus_tag="VWW99_001583"
     CDS             1190..1384
                     /locus_tag="VWW99_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9539398.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1468..2583
                     /gene="ychF"
                     /locus_tag="VWW99_001584"
     CDS             1468..2583
                     /gene="ychF"
                     /locus_tag="VWW99_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9539399.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELNDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPKAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2654..3223
                     /locus_tag="VWW99_001585"
     CDS             2654..3223
                     /locus_tag="VWW99_001585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9539400.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3224..6733
                     /gene="mfd"
                     /locus_tag="VWW99_001586"
     CDS             3224..6733
                     /gene="mfd"
                     /locus_tag="VWW99_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9539401.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEKNSI"
     gene            6791..7057
                     /locus_tag="VWW99_001587"
     CDS             6791..7057
                     /locus_tag="VWW99_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9539402.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7050..7418
                     /locus_tag="VWW99_001588"
     CDS             7050..7418
                     /locus_tag="VWW99_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9539403.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7538..8806
                     /locus_tag="VWW99_001589"
     CDS             7538..8806
                     /locus_tag="VWW99_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9539404.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8803..10080
                     /gene="tilS"
                     /locus_tag="VWW99_001590"
     CDS             8803..10080
                     /gene="tilS"
                     /locus_tag="VWW99_001590"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9539405.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKFKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10084..10626
                     /gene="hpt"
                     /locus_tag="VWW99_001591"
     CDS             10084..10626
                     /gene="hpt"
                     /locus_tag="VWW99_001591"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9539406.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10642..12600
                     /gene="ftsH"
                     /locus_tag="VWW99_001592"
     CDS             10642..12600
                     /gene="ftsH"
                     /locus_tag="VWW99_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9539407.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            12722..>12900
                     /locus_tag="VWW99_001593"
     CDS             12722..>12900
                     /locus_tag="VWW99_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539408.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYF"
BASE COUNT         4438 a         1928 c         2642 g         3892 t
ORIGIN      
        1 tcaattaata aaaatttatt tctacaagca ttaaatacta ctaagagagc tattagttct
       61 aaaaatgcca ttcctatttt atcaacagta aaaattgacg tgaccaatga aggtattact
      121 ttaattggtt caaatggtca aatttcaatt gaaaatttta tttctcaaaa aaatgaagat
      181 gctggtttgt taattacttc tttaggttcg atccttcttg aagcttcttt ctttatcaat
      241 gtagtatcta gtttacctga tgtaactctt gattttaaag aaattgaaca aaatcaaatt
      301 gttttaacca gtggcaaatc agaaattacc ctaaaaggaa aagatagcga acaatatcca
      361 cgaatccaag aaatttcagc aagcactcct ttaatacttg aaacaaaatt actcaagaaa
      421 attattaatg aaacagcctt tgctgcaagt acacaagaga gtcgtccgat tttaacaggt
      481 gtccacttcg tattgagtca acacaaagag ttaaaaacag ttgcaacaga ctctcatcgc
      541 ctaagccaga aaaaattgac tcttgaaaaa aatagtgatg attttgatgt cgtaattcct
      601 agccgttctc tacgcgaatt ttcagcggta tttacagatg atatcgaaac tgtagagatt
      661 ttctttgcca ataaccaaat cctctttaga agcgaaaata ttagcttcta tactcgtctc
      721 ctagaaggaa actatcctga tacagatcgc ttgattccaa cagactttaa cactactatt
      781 acttttaatg tggtaaactt acgccagtca atggagcgtg cccgtctttt atcaagtgcg
      841 actcaaaatg gtactgtgaa acttgaaatt aaggatgggg ttgttagcgc ccatgttcac
      901 tctccagaag ttggtaaagt aaacgaagaa atcgatactg atcaggttac tggtgaagat
      961 ttgaccatta gtttcaaccc aacttacttg attgattctc ttaaagcttt aaatagcgaa
     1021 aaggtgacta ttagctttat ctcagctgtt cgtccattta ctcttgtgcc agcagatact
     1081 gacgaagact tcatgcagct cattacacca gttcgtacaa attaagtgaa agaggttgag
     1141 cctggctcgc ctcttttatg atataatcga aaaagaaaag gagagtagta tgtatcaagt
     1201 tggaaatttt gttgagatga aaaaatcaca cgcttgtaca atcaagtcga ctggtaaaaa
     1261 ggctaatcgt tgggaaatta cacgtgtagg agcagatatc aaaataaaat gtagtaattg
     1321 tgagcatgtt gtcatgatgg ggcgatatga ttttgagcga aaaatgaata aaattattga
     1381 ctgagaaccc ttagttagag ggttagcact ttatcccttt ttgtgttata atattaggga
     1441 ttgaaatgaa aacggagaat gagaaatatg gctttgacag caggtatcgt tggtttgcca
     1501 aacgttggta aatcaacact atttaatgca attacaaaag caggagcaga ggcagcaaac
     1561 tacccatttg cgacgattga tccaaatgtt ggaatggtgg aagttccaga tgaacgccta
     1621 caaaaactaa ctgaaatgat aactcctaaa aagacagttc ccacaacatt tgaatttaca
     1681 gatattgcag ggattgtaaa aggagcttca aaaggagagg ggctagggaa taaattcttg
     1741 gccaatattc gtgaagtaga tgcgattgtt cacgtagttc gtgcttttga tgatgaaaat
     1801 gtaatgcgcg agcaaggacg tgaagacgcc tttgtagatc cacttgcaga tattgatacc
     1861 attaatctgg aattgattct tgctgactta gaatcagtga acaaacgata tgcgcgtgta
     1921 gaaaagatgg cacgtacgca aaaagataaa gaatcagtag cagaattcaa tgttcttcaa
     1981 aagattaaac cagtcctaga agacgggaaa tcagctcgta ccattgaatt tacagatgag
     2041 gaacaaaagg ttgtcaaagg tcttttcctt ttgacgacta aaccagttct ttatgtagct
     2101 aatgtggacg aggatgtggt ttcagaacct gactctatcg actatgtcaa acaaattcgt
     2161 gaatttgcag cgacagaaaa tgctgaagta gtcgttattt ctgcgcgtgc tgaggaagaa
     2221 atttctgaat tgaatgatga agataaaaaa gagtttcttg aagccattgg tttgacagaa
     2281 tcaggtgtag ataagttgac gcgtgcagct taccacttgc ttggattggg aacttacttc
     2341 acagctggtg aaaaagaagt tcgcgcttgg actttcaaac gtggtatgaa ggctcctaaa
     2401 gcagctggta ttatccattc agactttgaa aaaggcttta ttcgtgcagt aaccatgtca
     2461 tatgaagatc tagtgaaata cggatctgaa aaggccgtaa aagaagctgg acgcttgcgt
     2521 gaagaaggaa aagaatatat cgttcaagat ggcgatatca tggaattccg ctttaatgtc
     2581 taaaaattaa taaatggtgt taattaggtt ggaaaaaaat tccaaccctt ttggcttttg
     2641 aaaggaaaaa taaatgacca aattacttgt aggcttggga aatccagggg ataaatattt
     2701 tgaaacaaaa cacaatgttg gtttcatgtt gattgatcaa ctagcgaaga aacagaatgt
     2761 cacttttaca cacgataaga tatttcaagc tgacctagca tcctttttcc taaatggaga
     2821 aaaaatttat ctggttaaac caacgacctt tatgaatgaa agtggaaaag cagttcatgc
     2881 tttattaact tactatggtt tggatattga cgatttactt atcatttacg atgatcttga
     2941 catggaagtt gggaaaattc gtttaagagc aaaaggctca gcaggtggtc ataatggtat
     3001 caagtctatt attcaacata taggaactca ggtctttaac cgtgttaaga ttggaattgg
     3061 aagacctaaa aatggtatgt cagttgttca tcatgttttg agtaagtttg acagggatga
     3121 ttatatcggt attttacagt ctgttgacaa agttgacgat tctgtaaact actatttaca
     3181 agagaaaaat tttgagaaaa caatgcagag gtataacgga taaatggtga ccttattaga
     3241 tttattctca gaaaatgatc agattaaaaa atggcatcaa aatttaacag ataagaaaag
     3301 acaactaata cttggtttat caacatctac taaggctctt gcaattgcaa gcagtttaga
     3361 aaaagaagat aggattgtgt tattgacgtc aacttatgga gaagcagaag gacttgttag
     3421 tgatcttatt tctatcttgg gtgaggaact cgtctatcca tttttggtag atgatgctcc
     3481 tatggtggag tttttgatgt cttcacagga aaaaattatt tcacgggttg aagccttgcg
     3541 ttttttgact gattcatcta agaaagggat tttagtttgt aatatcgtag caagtcgatt
     3601 gattttaccg tctcccaatg cattcaaaga tagtattgta aaaatctcag ttggtgaaga
     3661 atatgatcaa cacgcgttta tccatcagtt aaaggaaaat ggctatcgaa aagttactca
     3721 agtacaaact cagggcgaat ttagtcttcg aggagatatt ttagatattt ttgaaatatc
     3781 ccagttagaa ccttgtcgaa ttgagttttt tggtgatgaa attgatggta tcaggtcatt
     3841 tgaagtagaa acacaattat cgaaagaaaa taagacagaa ctcactatct ttccagctag
     3901 tgatatgctt ttgagagaaa aggattatca acgaggacag tcagctttag aaaaacaaat
     3961 ttcaaaaact ttatcaccta ttttgaaatc atacctagaa gaaattcttt caagttttca
     4021 ccaaaaacaa agtcatgcag actctcggaa gtttttatct ttgtgctatg ataagacatg
     4081 gactgtcttt gattatattg aaaaagatac tccaatattc tttgatgatt atcaaaaatt
     4141 gatgaatcag tatgaagtct ttgaaagaga cttagcgcag tactttacag aagaattaca
     4201 gaatagtaaa gcattttctg atatgcagta tttttctgat attgaacaaa tctataaaaa
     4261 acaaagtcca gtgacctttt tctctaatct tcaaaagggt ttaggaaatc tcaaatttga
     4321 caaaatttat caattcaatc aatatcctat gcaggaattt ttcaatcagt tttcttttct
     4381 aaaagaagaa attgaacgat ataaaaaaat ggattacacc attattctgc agtctagcaa
     4441 ttcaatggga agtaaaacat tggaggatat gttagaggaa tatcagatta aattggattc
     4501 tagagataag acaagtatct gtaaagaatc tgtaaactta atagagggta atctcagaca
     4561 tggttttcat tttgtagatg aaaagatttt attgataact gaacatgaga tttttcaaaa
     4621 gaaattaaag cgtcgttttc gaagacaaca tgtttcaaat gcagagagat taaaagatta
     4681 caatgaactt gaaaaagggg actatgttgt ccatcatatc catgggattg gtcaatatct
     4741 aggaattgaa accattgaaa tcaagggaat tcatcgcgat tatgtcagtg tccagtacca
     4801 aaatggtgat caaatttcta ttcccgtgga acagattcat ctactgtcca aatatatttc
     4861 aagtgatggt aaagctccaa aactcaataa attaaatgac ggtcatttta aaaaggccaa
     4921 gcaaaaggtt aagaaccagg tagaggatat agctgatgat ttaatcaaac tctactctga
     4981 acgtagtcag ttgaagggtt ttgctttctc agctgatgat gatgatcaag atgcctttga
     5041 tgatgctttc ccttatgttg aaacggatga tcaacttcgt agtattgagg aaatcaagag
     5101 ggatatgcag gcttctcagc caatggatcg acttttagtt ggggatgttg gttttggaaa
     5161 gactgaagtt gctatgcgtg cagcctttaa agcagtcaat gatcacaaac aggttgtcat
     5221 tctagttccg acgacggttt tagcgcaaca gcactatacg aattttaagg aacgattcca
     5281 aaattttgca gttaatgttg atgtgttgag tcgctttaga agtaaaaaag agcagactgc
     5341 aacacttgaa aaattgaaaa acggtcaagt cgatattttg attggaacac atcgtgtttt
     5401 gtcaaaagat gttgtgtttg ctgatttggg cttgatgatt attgatgagg aacagcgatt
     5461 tggtgtcaag cataaggaaa ctttgaaaga actgaagaaa caagtggatg tcctaacctt
     5521 gaccgctacg ccaatccctc gtaccctcca tatgtctatg ctgggaatca gagatttgtc
     5581 tgttattgaa actccgccga ctaatcgcta tcctgttcag acctatgttt tggaaaagaa
     5641 tgatagtgtc attcgtgatg ctgtcttgcg tgaaatggag cgtggaggtc aagtttacta
     5701 tctttacaac aaagttgaca caattgttca gaaggtttca gaattacagg agttgattcc
     5761 ggaggcttcg attggatatg ttcatggtcg aatgagtgaa gtccagttgg aaaatactct
     5821 attagacttt attgagggac aatacgatat cttggtgacg actactatta ttgagacagg
     5881 ggtggacatt ccaaatgcta atactttatt tattgaaaat gcggaccata tgggcttgtc
     5941 aaccttatat cagttaagag gaagagtcgg tcgtagtaat cgtattgctt atgcttatct
     6001 catgtatcgt ccagaaaaat caatcagtga agtctctgaa aagagattag aagcgattaa
     6061 aggatttaca gaattgggct ctggctttaa gattgcaatg cgagatcttt cgattcgtgg
     6121 agcaggaaat cttttaggaa aatcccagtc tggtttcatt gattctgttg gttttgaatt
     6181 gtattcgcag ttattagagg aagctattgc taaacgaaac ggtaatgcta acgctaacac
     6241 aagaaccaaa gggaatgctg agttgatttt gcaaattgat gcctatcttc ctgatactta
     6301 tatttctgat caacgacata agattgaaat ttacaagaaa attcgtcaaa ttgacaaccg
     6361 tgtcaattat gaagagttac aagaggagtt gatagaccgt tttggagaat acccagatgt
     6421 agtagcctat cttttagaga ttggtttggt caaatcatac ttggacaagg tctttgttca
     6481 acgtgtggaa agaaaagata ataaaattac aattcaattt gaaaaagtca ctcaacgact
     6541 gtttttagct caagattatt ttaaagcttt atccgtaacg aacttaaaag caggcatcgc
     6601 tgagaataag ggattaatgg agcttgtatt tgatgtccaa aataagaaag attatgaaat
     6661 tttagaaggt ctgctgattt ttggagaaag tttattagag ataaaagagt ctaaggaaaa
     6721 aaattccatt tgatattttt cttctataaa atagataaaa atggtacaat aataaattga
     6781 ggtaataagg atgagattag ataaatattt aaaagtatcg cgaattatca agcgtcgtac
     6841 agtcgcaaag gaagtagcag ataaaggtag aatcaaggtt aatggaatct tggccaaaag
     6901 ttcaacggac ttgaaagtta atgaccaagt tgaaattcgc tttggcaata agttgctgct
     6961 tgtaaaagta ctagagatga aagatagtac aaaaaaagaa gatgcagcag gaatgtatga
     7021 aattatcagt gaaacacggg tagaagaaaa tgtctaaaaa tattgtacaa ttgaataatt
     7081 cttttattca aaatgaatac caacgtcgtc gctacctgat gaaagaacga caaaaacgga
     7141 atcgttttat gggaggggta ttgattttga ttatgctatt atttatcttg ccaactttta
     7201 atttagcgca gagttatcag caattactcc aaagacgtca gcaattagca gacttgcaaa
     7261 ctcagtatca aactttgagt gatgaaaagg ataaggagac agcatttgct accaagttga
     7321 aagatgaaga ttatgctgct aaatatacac gagcgaagta ctattattct aagtcgaggg
     7381 aaaaagttta tacgattcct gacttgcttc aaaggtgata aaatggaaaa tttattagac
     7441 gtaatagagc aatttttgag tttgtcagat gaaaagctgg aagaattggc tgataaaaat
     7501 caattattgc gtttacaaga agaaaaggaa aggaagaatg cgtaaattct taattatttt
     7561 gttgctacca agttttttga ccatttcaaa agtcgttagc acagaaaaag aagtcgtcta
     7621 tacttcgaaa gaaatttatt acctttcaca atctgacttt ggtatttatt ttagagaaaa
     7681 attaagttct cccatggttt atggagaggt tcctgtttat gcgaatgaag atttagtagt
     7741 ggaatctggg aaattgactc ccaaaacaag ttttcaaata accgagtggc gcttaaataa
     7801 acaaggaatt ccagtattta agctatcaaa tcatcaattt atagctgcgg acaaacgatt
     7861 tttatatgat caatcagagg taactccaac aataaaaaaa gtatggttag aatctgactt
     7921 taaactgtac aatagtcctt atgatttaaa agaagtgaaa tcatccttat cagcttattc
     7981 gcaagtatca atcgacaaga ccatgtttgt agaaggaaga gaatttctac atattgatca
     8041 ggctggatgg gtagctaaag aatcaacttc tgaagaagat aatcggatga gtaaagttca
     8101 agaaatgtta tctgaaaaat atcagaaaga ttctttctct atttatgtta agcaactgac
     8161 tactggaaaa gaagctggta tcaatcaaga tgaaaagatg tatgcagcca gcgttttgaa
     8221 actctcttat ctctattata cgcaagaaaa aataaatgag ggtctttatc agttagatac
     8281 gactgtaaaa tacgtatctg cagtcaatga ttttccaggt tcttataaac cagagggaag
     8341 tggtagtctt cctaaaaaag aagataataa agaatattct ttaaaggatt taattacgaa
     8401 agtatcaaaa gaatctgata atgtagctca taatctattg ggatattaca tttcaaacca
     8461 atctgatgcc acattcaaat ccaagatgtc tgccattatg ggagatgatt gggatccaaa
     8521 agaaaaattg atttcttcta agatggccgg gaagtttatg gaagctattt ataatcaaaa
     8581 tggatttgtg ctagagtctt tgactaaaac agattttgat agtcagcgaa ttgccaaagg
     8641 tgtttctgtt aaagtagctc ataaaattgg agatgcggat gaatttaagc atgatacggg
     8701 tgttgtctat gcagattctc catttattct ttctattttc actaagaatt ctgattatga
     8761 tacgatttct aagatagcca aggatgttta tgaggttcta aaatgaggga accagatttt
     8821 ttaaatcatt ttctcaagaa gggatatttc aaaaagcatg ctaaggcggt tctagctctt
     8881 tctggtggat tagattctat gtttctattt aaggtattgt ctacttatca aaaagagtta
     8941 gagattgaat tgattctagc tcatgtgaat cataagcaga gaattgaatc agattgggaa
     9001 gaaaaggaat taaggaagtt ggctgctgaa gcagagcttc ctatttatat cagcaatttt
     9061 tcaggagaat tttcagaagc gcgtgcacga aattttcgtt atgatttttt tcaagaggtc
     9121 atgaaaaaga caggtgcgac agctttagtc actgcccacc atgctgatga tcaggtggaa
     9181 acgattttta tgcgcttgat tcgaggaact cgcttgcgct atctatcagg aattaaggag
     9241 aagcaagtag tcggagagat agaaatcatt cgtcccttct tgcattttca gaaaaaagac
     9301 tttccatcaa tttttcactt tgaagataca tcaaatcagg agaatcatta ttttcgaaat
     9361 cgtattcgaa attcttactt accagaattg gaaaaagaaa atcctcgatt tagggatgca
     9421 atcttaggca ttggcaatga aattttagat tatgatttgg caatagctga attatctaac
     9481 aatattaatg tggaagattt acagcagtta ttttcttact ctgagtctac acaaagagtt
     9541 ttacttcaaa cttatctgaa tcgttttcca gatttgaatc ttacaaaagc tcagtttgct
     9601 gaagttcagc agattttaaa atttaaaagc cagtatcgtc atccgattaa aaatggctat
     9661 gaattgataa aagagtacca acagtttcag atttgtaaaa tcagtccgca ggctgatgaa
     9721 aaggaagatg aacttgtgtt acactatcaa aatcaggtag cttatcaagg atatttattt
     9781 tcttttggac ttccattaga aggtgaatta attcaacaaa tacctgtttc acgtgaaaca
     9841 tccatacaca ttcgtcatcg aaaaacagga gatgttttga ttaaaaatgg gcatagaaaa
     9901 aaactcagac gtttatttat tgatttgaaa atccctatgg aaaagagaaa ctctgctctt
     9961 attattgagc aatttggtga aattgtctca attttgggaa ttgcgaccaa taatttgagt
    10021 aaaaaaacga aaaatgatat aatgaacact gtactttata tagaaaaaat agataggtaa
    10081 aaaatgttag aaaacgatat taaaaaagtc ctcgtttcac acgatgaaat tacagaagca
    10141 gctaaaaaac taggtgctca attaactaaa gactatgcag gaaaaaatcc aatcttagtt
    10201 gggattttaa aaggatctat tccttttatg gctgaattgg tcaaacatat tgatacacat
    10261 attgaaatgg acttcatgat ggtttctagc taccatggtg gaacagcaag tagtggtgtt
    10321 atcaatatta aacaagatgt gactcaagat atcaaaggaa gacatgttct atttgtagaa
    10381 gatatcattg atacaggtca aactttgaag aatttgcgag atatgtttaa agaaagagaa
    10441 gcagcttctg ttaaaattgc aaccttgttg gataaaccag aaggacgtgt tgtagaaatt
    10501 gaggcagact atacttgctt tactatccca aatgagtttg tagtaggtta tggtttagac
    10561 tacaaagaaa attatcgtaa tcttccttat attggagtat tgaaagagga agtgtattca
    10621 aattagaaag aataatcttt aatgaaaaaa caaaataatg gtttaattaa aaatcctttt
    10681 ctatggttat tatttatctt tttccttgtg acaggattcc agtatttcta ttctgggaat
    10741 aactcaggag gaagtcagca aatcaactat actgagttgg tacaagaaat taccgatggt
    10801 aatgtaaaag aattaactta ccaaccaaat ggtagtgtta tcgaagtttc tggtgtctat
    10861 aaaaatccta aaacaagtaa agaaggaaca ggtattcagt ttttcacgcc atctgttact
    10921 aaggtagaga aatttaccag cactattctt cctgcagata ctaccgtatc agaattgcaa
    10981 aaacttgcta ctgaccataa agcagaagta actgttaagc atgaaagttc aagtggtata
    11041 tggattaatc tactcgtatc cattgtgcca tttggaattc tattcttctt cctattctct
    11101 atgatgggaa atatgggagg aggcaatggc cgtaatccaa tgagttttgg acgtagtaag
    11161 gctaaagcag caaataaaga agatattaaa gtaagatttt cagatgttgc tggagctgag
    11221 gaagaaaaac aagaactagt tgaagttgtt gagttcttaa aagatccaaa acgattcaca
    11281 aaacttggag cccgtattcc agcaggtgtt cttttggagg gacctccggg gacaggtaag
    11341 actttgcttg ctaaggcagt cgctggagaa gcaggtgttc cattctttag tatctcaggt
    11401 tctgactttg tagaaatgtt tgtcggagtt ggagctagtc gtgttcgctc tctttttgag
    11461 gatgccaaaa aagcagcacc agctatcatc tttatcgatg aaattgatgc tgttggacgt
    11521 caacgtggag tcggtctcgg cggaggtaat gacgaacgtg aacaaacctt gaaccaactt
    11581 ttgattgaga tggatggttt tgagggaaat gaagggatta tcgtcatcgc tgcgacaaac
    11641 cgttcagatg tacttgatcc tgcccttttg cgtccaggac gttttgatag aaaagtattg
    11701 gttggccgtc ctgatgttaa aggtcgtgaa gcaatcttga aagttcacgc taagaacaag
    11761 cctttagcag aagatgttga tttgaaatta gtggctcaac aaactccagg ctttgttggt
    11821 gctgatttag agaatgtctt gaatgaagca gctttagttg ctgctcgtcg caataaatcg
    11881 ataattgatg cttcagatat tgatgaagca gaagatagag ttattgctgg accttctaag
    11941 aaagataaga cagtttcaca aaaagaacga gaattggttg cttaccatga ggcaggacat
    12001 accattgttg gtctagtctt gtcgaatgct cgcgttgtcc ataaggttac aattgtacca
    12061 cgcggccgtg caggcggata catgattgca cttcctaaag aggatcaaat gcttctatct
    12121 aaagaagata tgaaagagca attggctggc ttaatgggtg gacgtgtagc tgaagaaatt
    12181 atctttaatg tccaaactac aggagcttca aacgactttg aacaagcgac acaaatggca
    12241 cgtgcaatgg ttacagagta cggtatgagt gaaaaacttg gcccagtaca atatgaagga
    12301 aaccatgcta tgcttggtgc acagagtcct caaaaatcaa tttcagaaca aacagcttat
    12361 gaaattgatg aagaggttcg ttcattatta aatgaggcac gaaataaagc tgctgaaatt
    12421 attcagtcaa atcgtgaaac tcacaagtta attgcagaag cattattgaa atacgaaaca
    12481 ttggatagta cacaaattaa agctctttac gaaacaggaa agatgcctga agcagtagaa
    12541 gaggaatctc atgcactatc ctatgatgaa gtaaagtcaa aaatgaatga cgaaaaataa
    12601 ccctgagaga ggctggagcc tctctttttt gtgcagttta ggagctaaag ggaacagaat
    12661 ggagaaaatg gaacaaatgt gttttctaat ctgttagact gtatctagaa aggggaaaat
    12721 tatgattaaa gaattgtatg aagaagtcca agggactgtg tataagtgta gaaatgaata
    12781 ttaccttcat ttatgggaat tgtcggattg ggaccaagaa ggcatgctct gcttacatga
    12841 attgattagt agagaagaag gactggtaga cgatattcca cgtttaagga aatatttcaa
//
LOCUS       DAVKHZ010000060        12423 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000060 DAVKHZ010000000
VERSION     DAVKHZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.060"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            62..547
                     /locus_tag="VWW99_001594"
     CDS             62..547
                     /locus_tag="VWW99_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9539409.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            564..1229
                     /locus_tag="VWW99_001595"
     CDS             564..1229
                     /locus_tag="VWW99_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9539410.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            1233..2096
                     /locus_tag="VWW99_001596"
     CDS             1233..2096
                     /locus_tag="VWW99_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9539411.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKSCDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            2098..2802
                     /locus_tag="VWW99_001597"
     CDS             2098..2802
                     /locus_tag="VWW99_001597"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9539412.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            2981..4654
                     /locus_tag="VWW99_001598"
     CDS             2981..4654
                     /locus_tag="VWW99_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9539413.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            4766..5857
                     /gene="ulaG"
                     /locus_tag="VWW99_001599"
     CDS             4766..5857
                     /gene="ulaG"
                     /locus_tag="VWW99_001599"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9539414.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            5971..7947
                     /gene="tkt"
                     /locus_tag="VWW99_001600"
     CDS             5971..7947
                     /gene="tkt"
                     /locus_tag="VWW99_001600"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9539415.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEEGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGENIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            8064..8363
                     /gene="yajC"
                     /locus_tag="VWW99_001601"
     CDS             8064..8363
                     /gene="yajC"
                     /locus_tag="VWW99_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9539416.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            8409..8837
                     /locus_tag="VWW99_001602"
     CDS             8409..8837
                     /locus_tag="VWW99_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9539417.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            8839..9249
                     /locus_tag="VWW99_001603"
     CDS             8839..9249
                     /locus_tag="VWW99_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539418.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            9549..12200
                     /gene="adhE"
                     /locus_tag="VWW99_001604"
                     /gene_synonym="adhC"
     CDS             9549..12200
                     /gene="adhE"
                     /locus_tag="VWW99_001604"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9539419.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLNQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         3941 a         2278 c         2649 g         3555 t
ORIGIN      
        1 tggacagaaa ctgagagtta tcgtttctgt ccagccccct ctttaaataa aggaggcaga
       61 tatgaattta aaacaagctt taattgacaa cgactcgatc cgactaggtt tagaggctaa
      121 cgattggaaa gaagcagtca aggtagcagt agatcccttg attgaaagtg gggcaatttt
      181 gccagagtat tacgatgcta tcattgaatc gactgaagag tatgggcctt actatatctt
      241 gatgccaggt atggctatgc cccacgctag acctgaagct ggtgtgcaaa gtgatgcctt
      301 ttcattaatt accttacaaa atcctgttgt attttcagat gggaaagagg tatctgtttt
      361 gttggcacta gcagcaacaa gttcaaaaat tcacacaagt gtagccattc cacaaattat
      421 tgccctgttt gaattagaag attctattgc acgtttacag gcttgccaga ctaaagaaga
      481 tgtcttggct atgattgaag aatctaagga tagcccttat ctcgaaggat tggatttgga
      541 aagttagaaa gaggaataaa gaaatgacaa aaagaatacc taatttacaa gttgcattag
      601 accattcaga cttgcaagga gcgattaaag cagctgtttc tgttggtcag gaagtagata
      661 ttatcgaagc tggaactgtt tgcttgcttc aagttggaag tgaactggct gaagtcttgc
      721 gtagcctttt cccagataag attattgtgg cagacacaaa atgtgctgat gctggtggaa
      781 cagttgctaa aaataatgcg gttcgtggag cagactggat gacttgtatc tgttgtgcaa
      841 ccatccctac tatggaagca gctctaaagg ctatcaagac tgaacgagga gaacgaggcg
      901 aaatccagat cgagctttat ggcgattgga cttttgaaca agctcagctt tggctagatg
      961 caggtatctc acaagctatt tatcaccaat ctcgtgatgc tcttcttgct ggtgaaactt
     1021 ggggtgaaaa agaccttaat aaggttaaaa aactcattga catgggcttc cgtgtatctg
     1081 taacaggtgg tctagatgta gatactctca aactctttga aggtgttgat gtctttacct
     1141 ttatcgcagg tcgtggaatt acagaggctg tggatccagc aggagcagcg cgtgccttca
     1201 aggatgaaat caaacgaatt tgggggtaaa tcatggtacg tccaattgga atttatgaaa
     1261 aggcaacccc aacacacttt acttggctag aacgtttaaa ttttgccaag gagttaggct
     1321 ttgattttat cgagatgtct attgacgaac gtgacgagcg tttagcaaga cttgactgga
     1381 gtaaggaaga acgcttggaa gttgtcaaag aaatctatga aactggtgtt cgtattcctt
     1441 ctatctgttt ttcaggccat cgtcgctacc cattgggatc aaaagattca gttctagagg
     1501 aaaaatctct agaactcatg aaaaaatgta tcgaattagc tcaagacttg ggagttcgta
     1561 cgattcaatt agctggttac gatgtttact atgaggaaaa gtcaccccag acacgccaac
     1621 gttttatcaa aaatttgaga aaatcctgtg actgggctga agaagctcag gtggtacttg
     1681 ctattgaaat tatggatgat cctttcatca gtagcatcga aaaatatttg gctatagaaa
     1741 aagagattga ctctcccttc ctctttgtat atccagatat tggtaatgtg tctgcatggc
     1801 ataatgatat ctatagtgag ttttatcttg gtcatcatgc catcgcagct ctccatctca
     1861 aggatactta tgcagtgaca gaaagttcaa agggccagtt ccgagatgta cctttcgggc
     1921 aaggttgtgt caaatgggaa gaagctttcg atattttaaa ggaaaccaat tataatggac
     1981 ctttcctaat cgaaatgtgg tctgaaaatt gtgaaacagt agaagaaaca cgcgcagcca
     2041 ttcaagaggc gcaagctttt ctctatccac tcattaagaa agcaggtttg atgtaagatg
     2101 aatcaagtaa tcaatgctat gcgtaaacga gtctgtgatg ccaatcaatc attgccaaaa
     2161 catggacttg tcaaatttac ctgggggaat gtatctgaag ttaatcgcga actcggtgtc
     2221 attgttatca aaccatcagg cgtggattat gacgaattga cacctgaaaa catggtagtg
     2281 actgatctag atggtaagat cctagaaggg gatttaagac catcttccga cctcccaact
     2341 catgtgcaat tatataaggc ttggtcagaa attggtagtg tggttcacac ccattcgaca
     2401 gaagctgttg gttgggctca ggcaggtcgt gatattcctt tctacggaac aacccatgca
     2461 gattatttct acggttcaat cccttgcgcc cgtagtttga ccaaggacga agtagaagtg
     2521 gcctatgaaa aagatactgg cctggttatc gtagaagagt ttgaacatcg cggacttaac
     2581 ccggttgaag taccaggaat tgttgtacgc aatcacggtc cattcacctg gggcaaaaat
     2641 ccagagaatg ctgtttatca ctctgtcgta ctagaggaag tatcaaagat gaatcgcttt
     2701 acagaacaaa tcaatccaag agttggacct gctccccagt acatactaga aaaacactac
     2761 caacgtaaac atggaccaaa tgcttattat ggtcaaaagt aagaacgatg aaggaggaga
     2821 aaaagataaa tttagctcct ctttttacat ttgattttta ttgagagtaa agttggaatt
     2881 gaagtaattt taaaagattt tttagaaata gcgcttgata tatatatggt aaaataaaaa
     2941 gaattgctgt gatatcaata gatttggggg attttttaat atggtactgg ataaggcaag
     3001 ttgtgatttg cttcaatatt tgatggatca agaaacgtcc aaaacgatta tggcgatttc
     3061 gaaagatttg aaagagtcaa gaaggaaaat ttattatcac attgacaaaa tcaatgctgc
     3121 tctgggtgac gaggcgcttc acatcattag tattccacga attggtattc acttaacgga
     3181 agagcagaga gatgcttgtt gtaaactatt atcggaagta gattcgtacg attatatcat
     3241 gagtgcgcat gaacgtatga tgataatgtt actatggata ggtatttcta aagaacgtat
     3301 tacgattgaa aaattgatag agttaacaga ggtatctagg aatactgttc tcaatgattt
     3361 gaatagtatt cgttatcaac taactttgga acaatatcag gtgatcttgc aagtgagcaa
     3421 gtcacaggga taccaccttc atgcccaccc tcttaataaa attcagtatc ttcaatcgct
     3481 tctatatcat atttttatgg aagaaaatgc cacttttgta tctattttag aagataagat
     3541 gaaagagagg ttagatgatg agtgtttgct ttctgttgaa atgaaccaat tttttaagga
     3601 acaggttcct ttagttgaac aagatttagg gaagaaaata aaccatcatg aaataacttt
     3661 tatgttgcag gttctacctt atttgctgtt aagctgtcat aatgttgaac agtatcaaga
     3721 aagacatcag gatatagaga aagaattttc tttgataaga aaaagaatag agtatcaggt
     3781 gtctaagaaa ttaggagaac ggttgtttca aaagtttgaa atttctttgt caggacttga
     3841 agtttctctt gtagctgttc tcctcctctc ctatcgtaaa gatttggata ttcatgcaga
     3901 aagtgatgat tttcggcaat taaaacttgc tttagaagaa tttatctggt attttgaatc
     3961 acaaatccga atggagattg agaacaagga tgatttgtta cgaaatttga tgatccactg
     4021 taaagccttg ttatttagaa agacttacgg tattttttct aaaaatcctc taacaaaaca
     4081 aattcgatcc aagtatggag aattattttt agtcactaga aaatctgcgg aaattttaga
     4141 aggagcatgg tttattcggc taacagacga tgatattgcc tatttgacga ttcatattgg
     4201 aggattttta aaatatacac catcgtctca aaaaaatatg aaaaaagttt atctcgtttg
     4261 tgatgaaggt gttgcggttt cgagactttt gctgaaacaa tgcaaacttt attttccaaa
     4321 tgagcaaatt gacactgtat ttacaacaga acaatttaag agtgtggaag atattgcaca
     4381 agttgatgta gtgattacta ctaatgatga tttggatagc agatttccga ttttaagggt
     4441 taatcctatc cttgaagcag aagatatttt gaaaatgcta gactatctta aacacaatat
     4501 atttcgtaat aagagcaaaa gtttcagtga aaatctttct agtcttattt cgtcttatat
     4561 tgtagacagc aagttggcta gtaagttcca agaagaggtt caaacactta taaatcaaga
     4621 aatagtagtt caagcttttt tggaagatat ttgaaggaca gtccaatgat gaacacaaac
     4681 ctgtgttttt cttggtcttt tttagtgttt tgaagggtgg tatactaatc tcaaagataa
     4741 caattatatc caaaggaggc aacatatgcc aaacgtcaaa gaaattacaa gagagtcatg
     4801 gattttagcc actttcccag agtggggaac atggttgaac gaagaaatcg aagaagaagt
     4861 cgtacctgaa ggcaactttg ccatgtggtg gctaggcaac tgtggtactt ggattaagac
     4921 accagctggt gctaacgttg tcatggacct ttggtcaaac cgtggaaaat caaccaaaaa
     4981 agtgaaagat atggttcgtg ggcaccaaat ggcaaatatg gcaggtgttc gtaagttgca
     5041 accaaacttg cgtgttcagc caatggttat cgatccattt gctatcaacg aactagacta
     5101 ttacttagtt tcacacttcc acagtgatca tatcgaccca tacacagctg cagcaattct
     5161 caacaatcct aagttagagc atgttaagtt tatcggtcct taccactgtg gacgaatctg
     5221 ggaaggatgg ggtgttccaa aagaacgtat catcgttgtt aaaccaggtg acactatcga
     5281 attaaaagat atgaagattc atgcagtaga atcatttgac cgtacttgct tggtaactct
     5341 cccagtgaac ggtgctgatg agacaggcgg tgaacttgct ggcttggctg ttacagatga
     5401 agaaatggct caaaaggctg ttaactatat ctttgaaaca ccaggtggaa ccatctatca
     5461 tggtgcagat tctcacttct caaactattt tgcaaaacat ggtaaagact ttaaaattga
     5521 tgttgctttg aataactatg gtgaaaatcc ggtaggtatc caagacaaaa tgacatctat
     5581 cgaccttctt cgtatggcag aaaatctgcg taccaaagtc attatcccag ttcactatga
     5641 tatctggtct aacttcatgg cttctactaa tgagattcta gaactttgga aaatgcgaaa
     5701 agatcgcttg caatacgatt tccatccatt tatctgggaa gttggcggta agtacactta
     5761 tcctcaagat caacacttag tagaatacca tcatccacgt ggttttgatg attgttttga
     5821 acaagactct aacattcaat ttaaagcttt gctataatat aaaaatattc tttggaggtt
     5881 atccgtatgt tgcatgatac ggataatttt catataatag taaaatagaa tgtgtgattc
     5941 aataatcacc tcaaatagaa aggaaattct atgtcaaatc tatctgttaa tgcaattcgt
     6001 tttctaggta ttgacgccat taataaagcc aactcaggtc atccaggtgt ggttatggga
     6061 gcggctccga tggcttacag cctctttaca aaacaacttc atatcaatcc agctcaacca
     6121 aactggatta accgcgaccg ctttattctt tcagcaggtc atggttcaat gctcctttat
     6181 gctcttcttc acctttctgg ttttgaagat gtcagcatgg atgagattaa gagtttccgt
     6241 caatggggtt caaaaacacc aggtcaccca gaatttggtc atacggcagg gattgatgct
     6301 acgacaggtc ctctagggca aggtatttca actgctactg gttttgccca agcagaacgt
     6361 ttcttggcag ccaaatataa ccgtgaaggc tacaatatct ttgaccacta tacttacgtt
     6421 atctgtggag acggagactt gatggaaggt gtctcaagcg aggcagcttc atacgcaggt
     6481 ttgcaaaaac ttgataagtt ggttgttctt tatgattcaa atgatatcaa cttggatggt
     6541 gagacaaagg attcctttac agaaagtgtt cgtgaccgtt acaatgccta cggttggcat
     6601 actgccttgg ttgaaaatgg aacagacttg gaagccatcc atgctgctat cgaaacagca
     6661 aaagcttcag gcaagccatc tttgattgaa gtgaagacgg ttattggata cggttctcca
     6721 aacaaacaag gaactaatgc tgtacacggc gcccctcttg gagcagatga aactgcatca
     6781 actcgtcaag ccctcggttg ggactacgaa ccatttgaaa ttccagaaca agtatatgct
     6841 gatttcaaag aacatgttgc agaccgtggc gcatcagctt atcaagcttg gactaaatta
     6901 gttgcagatt ataaagaagc tcatccagaa ctggctgcag aagtagaagc catcatcgac
     6961 ggacgtgatc cagtcgaagt gactccagca gacttcccag ctttagaaaa tggtttttct
     7021 caagcaactc gtaattcaag tcaagatgcc ttgaatgttg tggcagctaa attaccaacc
     7081 tttctaggtg gatcagctga ccttgctcac tcaaacatga cttatatcaa gacggatgga
     7141 cttcaagacg atgccaatcg cttgaatcgt aacattcagt ttggtgttcg tgaatttgca
     7201 atgggaacaa tcttgaacgg gatggccctt catggtggac ttcgtgtata cggtggaact
     7261 ttcttcgtct tctctgacta tgtgaaggca gctgtccgct tgtcagcctt acaaggactt
     7321 cctgtgactt atgtctttac ccatgattca atcgcagttg gggaagatgg tccgactcac
     7381 gaaccagttg agcatttagc aggtcttcgt gctatgccaa atctaaatgt tttccgtcca
     7441 gcagatgcgc gtgaaacgca agcagcttgg taccttgcag tgacaagtga gaaaacacca
     7501 actgcccttg tcttgacacg tcaaaacttg actgtcgaag agggaacaga cttcgacaag
     7561 gttgctaaag gtgcttatgt tgtatatgaa aatgcagccg actttgatac catcttgatt
     7621 gcgacaggtt cagaggttaa tcttgctgtc tcagctgcta aagaattggc tagtcaaggc
     7681 gaaaacatcc gcgtagtcag catgccatct acagatgtct ttgataaaca agatgcagct
     7741 tacaaggaag aaattcttcc aaatgcagtc cgccgtcgtg ttgcagtcga aatgggtgca
     7801 agtcaaaact ggtacaaata tgttggtctc gatggtgccg ttctaggtat tgataccttc
     7861 ggagcctctg ccccagcacc aaaagtattg gcagaatatg gctttactgt cgaaaatctt
     7921 gtaaaagttg ttcgaaattt gaaataatcc taaaaatcag ggcgtaagct ctggtttttc
     7981 ttaccagaaa agtaaggtac aatcttgtaa aagtagctga aatttgatat agtagtccta
     8041 tgtaaaagac aaaggagaaa acaatgaatc caaatattac ttttttaatc atgcttgtag
     8101 gtatgatggc cttgatgttc tttatgcaac gttctcaaaa gaaacaagct caaaagcgta
     8161 tggaaagctt aaataaacta caaaaaggct atgaagtgat tacaatcggt ggactttatg
     8221 gaacagtcga tgaagtagat acggagaagg gaacaatagt tcttgacgta gatggagttt
     8281 acttgacttt tgaactagct gctatcaaga cagtattacc gctgaaagaa acagcttcac
     8341 tagaaggcgc aattgaaaaa taagacggga tcacgaactc ccgttttcta taaaagaaag
     8401 gaaatgggat gaaaaaatta gtctttgtct gtctgggaaa tatttgccgt agccctatgg
     8461 ccgagtttgt tatgaaatca atgacagata actacgaaat ccaaagtcga gcaacttcct
     8521 cttgggaaca tggcaatccg attcataagg ggactcaggg aatttttcaa gagtatgaga
     8581 ttccttatga caagaacaag acatcgcttc agattagtaa ggaagatttt gaagcctttg
     8641 attatattat cggaatggac gcttcaaata tttctgactt acgtcagatg tgtccagtag
     8701 actgtcaaga taagatttac tcattttcat ctgaaagtgt tccagaccct tggtatacag
     8761 gagattttga agaaacctat cgacgtgttc aagagggctg tcaagcttgg ttagaacgtt
     8821 tagaaaagga gagttaaggt ggaaaatctt acgaattttt acgaaaagta tcgtgtctat
     8881 ctgactcgtc cacgtttaga gcttttggca gtagttacca ttgttttctg tgctgtactc
     8941 gtcttttttc taaatattcc aggaaaaggt gtcttaaaac tcgataatgg aacgattgtt
     9001 tatgatggca gtcttgtccg tggtaaaatg aatggccaag gtaccattac cttccaaaat
     9061 ggagaccaat atacaggtgg cttcaacaat ggagccttca acggaaaagg tacctttcaa
     9121 tctaaagaag gctggaccta cgaaggtgat tttgtaaatg gtcaggctga aggaaaaggg
     9181 aaactaacaa cagaacaaga agtcgtttat gaaggaactt ttaaacaagg cgtttttcaa
     9241 caaaaataaa gcctccttat caaaggaggt attattagaa tcacaaggta agcgcttacc
     9301 tgtaaatccc tttctttcca aatccctctt ccaagcaagt ttgtgaaata aaaaatattt
     9361 gaaataaatt tcacaaactt caaagataaa acttgataag aaaagaaaat gagaaaagtt
     9421 tcacaagagt ttaaaaattc tttgtgaaat gtttatgaaa ctgtttacaa agttgaaata
     9481 atgcgttata ataaacttgt gaaaaaatta acaaaggata tcgttccttg aaagctatgg
     9541 aggaaaatat ggctgataaa aaaactgtga caccagagga aaagaaactc gttgctgaaa
     9601 agcacgtaga tgagttggtt caaaaagctc tagttgccct tgaagaaatg cgtaaattga
     9661 atcaagagca agttgactac atcgttgcca aagcatcagt agcagctttg gatgcccatg
     9721 gagaattggc tttacatgcc tttgaagaaa caggacgtgg tgtatttgaa gataaagcaa
     9781 ctaagaactt gtttgcttgt gaacacgtag taaacaacat gcgccacact aagacagttg
     9841 gtgttatcga agaagacgat gtaacaggat tgactcttat tgctgaacca gttggtgttg
     9901 tttgtggtat tactccaaca acaaacccaa catcaacagc aatcttcaaa tcattgattt
     9961 cattgaagac acgtaaccca atcgtctttg ccttccatcc atcagcacaa gaatcatctg
    10021 ctcatgcagc tcgtatcgtc cgcgatgcag ctatcgcagc tggtgctcct gaaaactgtg
    10081 tgcaatggat tactcaacca tctatggaag caacaagtgc ccttatgaac cacgaaggtg
    10141 ttgcgacaat ccttgcaaca ggtggtaatg ccatggttaa ggcggcttat tcatgtggta
    10201 aaccagctct tggggtaggt gccggaaacg ttccagctta tgttgaaaaa tcagcaaaca
    10261 ttcgtcaagc agcacacgat atcgtcatgt ctaaatcatt tgataacggt atggtctgtg
    10321 catctgaaca agcagttatc attgataaag aaatttacga tgaatttgta gcagagttca
    10381 aatcttacca cacttacttt gtaaacaaaa aagaaaaagc tcttcttgaa gagttctgct
    10441 tcggcgtcaa agcaaacagc aaaaactgtg ctggtgcaaa attgaacgct gacatcgttg
    10501 gtaaaccagc aacttggatt gcagaacaag caggatttac agttccagaa ggaacaaaca
    10561 ttcttgctgc agaatgtaaa gaagttggcg aaaatgagcc attgactcgt gaaaaattgt
    10621 caccagttat tgcagttttg aaatctgaaa gccgtgaaga tggtattact aaggctcgtc
    10681 aaatggttga atttaacggt cttggacact cagcagctat ccacacagct gacgaagaat
    10741 tgactaaaga atttggtaaa gctgttaaag ctattcgtgt tatctgtaac tcaccttcta
    10801 cttttggtgg tatcggggac gtttacaatg ccttcttgcc atcattgaca cttggatgtg
    10861 gttcttacgg acgcaactca gttggggata acgttagtgc cattaacctc ttgaatatca
    10921 aaaaagtcgg aagacggaga aataacatgc aatggatgaa acttccttca aaaacatact
    10981 ttgaacgtga ttcaattcaa taccttcaaa aatgtcgtga cgttgaacgt gtcatgatcg
    11041 ttactgacca tgccatggta gagcttggtt tccttgatcg tatcatcgaa caactggacc
    11101 ttcgtcgcaa taaggttgtt taccaaatct ttgcggatgt agaaccggat ccagatatca
    11161 caactgtaaa ccgtggtact gagattatgc gtgccttcaa accagatacc atcatcgcac
    11221 tcggtggtgg gtctccaatg gatgctgcca aagtaatgtg gctcttctac gagcaaccag
    11281 aagtggactt ccgtgacctt gtccaaaaat tcatggatat ccgtaaacgt gccttcaagt
    11341 tcccattgct tggtaagaag actaaattca tcgcgattcc aactacatct ggtacaggat
    11401 ctgaagtaac accatttgcc gttatctctg ataaagcaaa caaccgtaaa tacccaatcg
    11461 ctgactactc attgacacca actgtggcaa tcgtagatcc tgctttggta ttgacagttc
    11521 caggatttgt tgctgctgat actggtatgg acgtattgac tcacgcgaca gaagcatacg
    11581 tatcacaaat ggctagtgac tacactgatg gtttagcact tcaagccatt aaattggtct
    11641 ttgaaaatct cgaaagctca gttaagaatg cagacttcca ctcacgtgag aaaatgcata
    11701 acgcttcaac aatcgctggt atggcctttg ccaatgcctt cctaggtatt tctcactcaa
    11761 tggcccataa gattggtgcg caattccaca caatacacgg tcgtacaaat gctatcttgc
    11821 ttccatacgt tatccgttac aacggtacac gtccagctaa gacagcaaca tggcctaagt
    11881 acaactacta ccgtgcagat gaaaaatacc aagatatcgc acgcatgctt ggacttccag
    11941 cttctactcc agaagaaggg gttgaatctt acgcaaaagc tgtctacgaa ctcggtgaac
    12001 gtattgggat ccaaatgaat tttagagacc aaggaattga cgaaaaagaa tggaaagaac
    12061 attctcgtga attagccttc ctggcttatg aagaccaatg ttcaccagct aacccacgtc
    12121 ttccaatggt agaccatatg caagaaatca tcgaagatgc atactatggc tacaaagaaa
    12181 gaccaggacg ccgtaaataa ttgtttatta gtctagaagc aagacaaaaa ctcaatttga
    12241 gggaaagatc cagtaatttt tctatgataa aaggcatcct atcaaggttt ttgaacacct
    12301 gataggatgc ctttttatga tattgaggcc tttttgccct ttttgaaaaa ctagaataga
    12361 aacaaaatat ataatagatt gaaactagaa tagtacacat ctgcttctaa aacattgtta
    12421 gaa
//
LOCUS       DAVKHZ010000061        12406 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000061 DAVKHZ010000000
VERSION     DAVKHZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.061"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            27..836
                     /locus_tag="VWW99_001605"
     CDS             27..836
                     /locus_tag="VWW99_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9539420.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            841..1542
                     /locus_tag="VWW99_001606"
     CDS             841..1542
                     /locus_tag="VWW99_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9539421.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1567..2484
                     /locus_tag="VWW99_001607"
     CDS             1567..2484
                     /locus_tag="VWW99_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU9539422.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2512..4053
                     /locus_tag="VWW99_001608"
     CDS             2512..4053
                     /locus_tag="VWW99_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU9539423.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4072..4530
                     /locus_tag="VWW99_001609"
     CDS             4072..4530
                     /locus_tag="VWW99_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9539424.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4532..6754
                     /locus_tag="VWW99_001610"
     CDS             4532..6754
                     /locus_tag="VWW99_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9539425.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6794..7903
                     /locus_tag="VWW99_001611"
     CDS             6794..7903
                     /locus_tag="VWW99_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9539426.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8060..8950
                     /locus_tag="VWW99_001612"
     CDS             8060..8950
                     /locus_tag="VWW99_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9539427.1"
                     /translation="MKIIGIDIGGTTIKADLYDDFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYVLNYSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTSEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            8975..9298
                     /locus_tag="VWW99_001613"
     CDS             8975..9298
                     /locus_tag="VWW99_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539428.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            9288..11279
                     /locus_tag="VWW99_001614"
     CDS             9288..11279
                     /locus_tag="VWW99_001614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9539429.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            11295..11771
                     /locus_tag="VWW99_001615"
     CDS             11295..11771
                     /locus_tag="VWW99_001615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU9539430.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11805..12386
                     /locus_tag="VWW99_001616"
     CDS             11805..12386
                     /locus_tag="VWW99_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539431.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
BASE COUNT         4210 a         1787 c         2450 g         3959 t
ORIGIN      
        1 acgtttactt caactaagga gctcatatgt tactgcaaaa agaactaatt ccaatgatag
       61 aagctaactt accaaatatg gcatatgctg aaaaagacat tgctaaattc ttcttaaaac
      121 agcaacctct gaatgattat tcatctaaag cattgtgcga ataccttaat gtatccaaag
      181 caacattgac tcgatttgcg aaaaaatgtg gttttaaagg ttttagacaa ttcattttca
      241 aataccaaga gatgattcat gagaaagaaa agttggcatt atatacagag gcaacagaaa
      301 aagttttatc cgactatgag gaaatgttga gaaaaactta cacggttctt gatgaagttc
      361 aacttgagcg tattgctgag atgatagaaa ctgctgagcg tgtatatctc tacggtaaag
      421 gaagttctgt tcttgcttta caagaaatga agatgagatt tatgcgtctc ggagtgattg
      481 gtgaagtatt atcagacgag gatatgattt tgtggagtag cttactactt aatgaaaatt
      541 gccttgtcat tggagcatcc atttcaggtc aaactgatat tgtactagaa ggtctacaaa
      601 aagctgcaga taaaggcgct aaaacagttt taatgactac aagaaaattt gacgaagaag
      661 attgtttctt tgatgaacta ttgttattag cttcgaccga tcatctctcg tatggcaatc
      721 gcatatcacc tcagtttcca atacttttaa ttacagactg cttattctct aattatctgg
      781 aaagtccaga gagacaatat tattacaatc aaactattat ccataaggag gaataataaa
      841 atgagccaaa tttggactaa agaaaaattt ataagccaag ttcaaggtgg agtcattgtt
      901 tcttgtcaag ctttacctgg tgaagccctt tataatgaag aatttagctt gatgcctttt
      961 atggctaaag cagctttaga ggcaggagca gtgggcattc gcgcaaattc tgtgcgtgat
     1021 attaaagcaa ttcagaaagt agtagattta ccaataattg gaattatcaa aagggattat
     1081 ccacctcaag aaccatatat tactgctacg atgaaagaag tagatgaact tgtagaatgc
     1141 ggaacaacag tcattgcatt tgatgcaact ttaagaccaa gatatgatgg cttagttgtc
     1201 agtgaattta tcaaaaaaat aaaagaaaaa tatccgaatc aattgctgat ggcggatgta
     1261 agtaatttag atgaaggtct ctatgcattt aaatcaggcg ttgattttgt tggtacaaca
     1321 ttatcaggtt acacaagtac aagtgtacaa tcagatgagc ctgattttga actaatgaaa
     1381 aaattggctg attttaatat tccggtaatt gccgaaggaa aaattcatta tccagaacaa
     1441 ttaaaaaaag cttatagttt aggtgttacc agtgtagtca ttggtggagc gattacacgt
     1501 ccaaaagaaa ttgctcagcg atttattaat gtcatcaaat aaaaaacaat ataaaggaga
     1561 aatctgatga aagatttaac taaatacaaa ggcgttatcc ctgcatttta tgcttgctat
     1621 gatgaaaatg gtgaaattag ccaagatcgt gtaaaatctc tggtacaata tttcattgac
     1681 aaaggtgtaa aaggtatcta tgtaaatggt tcttcaggtg aatgtattta ccaaagtgta
     1741 gaagatcgta aacaaattat tgaagctgtt atggaagttg ctaaaggtaa attaacagtt
     1801 atcaaccata ttgcatgtaa taacacgaaa gatagtatcg aattggcaaa acattcagaa
     1861 agtgttggag tcgatgctat tgcagctatc ccacctattt atttcaaatt gccagagtat
     1921 tcaatcgcag catattggaa tgcaatgagt gaagctgcgt caaatacaga ttttattatc
     1981 tataatattc cacaattggc aggggttgcg ttgactggta gtttgtatgc aacaatgcgt
     2041 caaaatcctc gtgtgattgg agttaaaaat tcttctatgc ctgtacaaga tattcaaatg
     2101 tttgtagctg caggtggaga agattacatt gtattcaatg gtccagatga acaatatctt
     2161 ggtggtcgct tgatgggagc agaagctggt attggtggta cttatggcgt tatgccagat
     2221 ttgttcttga aattggaaag tttgattcaa gaacgagatt tagatacagc taaaaaactt
     2281 caatatgcta tcaatgaagt tatctataag atgatatcag gtaaggcaaa tatgtatgct
     2341 gtagcaaaag aagttttgcg tctaaatgaa aaacttgatt taggttctgt tcgtcaacct
     2401 ttagaagcat tggcagaagg tgacttggaa gttgcaaaac aagcagcaga acttattcaa
     2461 caagcacgaa aagaattttt ataataaata aaatcaatat ggagatatgt tatgggcaca
     2521 acaggattta caataattga cttaattatc ttgattgttt atttacttgc ggtgttggtt
     2581 gcaggtatct atttctctaa aaaagagatg aaaggaaaag agttctttaa aggagatggt
     2641 tcggttcctt ggtatgttac ttcggtatcc atttttgcca caatgctcag tccgatttcc
     2701 ttcttgggac tcgctggtag ctcttatgca ggtagctgga ttttatggtt tgctcaatta
     2761 gggatggtag tagctattcc actgacaatt cgttttatct tacctatctt tgcacggata
     2821 gacatcgata cggcatatga ttacttggat aaacgtttta attctaaagc acttcgtatt
     2881 atttcagcac tcttgtttat tatttatcaa ttgggacgta tgtctatcat tatgtacctc
     2941 ccatcagctg gtttatcagt attgacagga attgacatca atattttgat tattttgatg
     3001 ggtgtagttg caattgttta ttcttatact ggtggtctaa aatccgtatt atggacagac
     3061 tttattcaag gtgtgattct gattagtggt gtcgttttag ctttatttgt actgattgct
     3121 aatattaaag gtggctttgg tgcagtagca gaaacattag caaacgggaa attccttgct
     3181 gcaaatgaaa aacttttcga tcctaacttg ctttcaaact ccatcttttt aattgtgatg
     3241 ggttcaggct ttacaatctt gtcttcctat gcttcatctc aagatttggt tcaacgtttt
     3301 actacaacac aaaatattaa gaaacttaat aagatgttgt tcacaaacgg tgttttgtca
     3361 cttgcaactg caacagtctt ttacttgatt ggtacaggct tgtacgtatt ctatcaagta
     3421 caaaatgcag atagtgcagc tagcaatatc cctcaagacc aaatctttat gtactttatt
     3481 gcataccagt taccagtagg tatcacaggt ttgatcttgg cagcgattta tgcagcatct
     3541 caatcaacta tttcaacagg tttgaactct gttgcaactt catggacatt ggatattcaa
     3601 gatgtcattt ctaaaaatat gtcagacaat cgtcgtacga aaattgcaca attcgtatct
     3661 ctagcagtag gtttattctc aattggtgtt tccattgtca tggctcactc agatattaaa
     3721 tctgcatacg aatggttcaa tagtttcatg ggacttgtac ttggtctact tggtggtgta
     3781 tttattcttg gatttgtttc taaaaaagca aataaacaag gtgcttatgc agcgctgatt
     3841 gtatcaacca tcgtcatggt atttattaaa tacttccttc ctccaacagc tgttagctac
     3901 tgggcatatt cattgatttc aatctctgta tcagtagttt caggttatat tgtatctgtt
     3961 cttactggaa ataaagtatc tgcacctaaa tatacaacga ttcatgatat tacagaaatt
     4021 aaagcggatt caagttggga agttcgtcac taatagaaaa gagaaaataa tatgattatt
     4081 tctgaacaat ctgattttaa acgatatgct tctgtcaata aatatttttc aaaagtctgt
     4141 gattttttag aaaatacaaa tttgacagat ttagttgatg gaaaaattga tattgacggg
     4201 gaaaatgttt ttgcaaattg tatgacttat ctagctgatg gagttccagg agatattttt
     4261 gaaactcata agaaatattt agatattcat attgttgttg aaaatactga aaaaatggca
     4321 gtaacttccc cagttcgtgc ccagtcacgt gtacctttta gcgaagagaa ggatattgct
     4381 ttttacgata gcaaagacta tcaaattgtt gaattgcttc cagggaatat gttggtgact
     4441 tttgaagaag atcttcacca accaaagatt cattgcaatg atgaaactgt taaaaaactt
     4501 gttatcaaag ttttaaacga ggaaaaataa aatgaaaaaa aatattaaac aatatgtaac
     4561 cttaggtact gtagtggtat tatcagcatt tgttgctaac tcagttgcag ctcaggagac
     4621 tgaaacttct gaagtatcaa caccaaagtt ggtgcaacct gttgcaccaa cgactccgat
     4681 ttcggaagta caacctacat cggataactc ttcggaagtt actgtacaac ctcgaacagt
     4741 tgaaactact gttaaggatc catcttctac agcggaagaa actcctgtct tagaaaaaaa
     4801 taatgttact ttaacagggg gcggagaaaa tgttactaaa gagttaaagg ataaatttac
     4861 tagcggtgac tttactgtag tgattaagta caatcagtca agtgagaaag gcttacaagc
     4921 tctgtttgga atatctaatt ccaaacccgg tcaacaaaat agttatgtag atgtgttcct
     4981 tagagacaat ggtgagttgg ggatggaagc gcgtgatact tcttccaata aaaataacct
     5041 agtatccaga cctgcttcag tttggggtaa gtacaaacaa gaggctgtga ctaacactgt
     5101 tgcagtagta gcagattcag tcaaaaaaac atattcttta tacgcaaatg gtacaaaagt
     5161 agtagaaaag aaagtggata atttcctaaa catcaaggat attaaaggta ttgattacta
     5221 tatgcttggg ggagtgaaac gtgcaggaaa aacggcgttt ggttttaacg gaacactaga
     5281 aaatatcaaa ttctttaata gtgcattgga tgaagaaact gttaaaaaga tgacaacaaa
     5341 cgctgttact ggacatttaa tttatacggc taatgataca acaggttcta actatttccg
     5401 tattccagtt ctgtatactt ttagcaatgg tcgggtattt tcaagcattg acgctcgtta
     5461 cggtggaact catgatttct tgaataaaat taatattgct acaagttata gtgatgataa
     5521 tggtaagaca tggactaaac caaaattaac attggcattc gatgattttg cgccagtacc
     5581 attagaatgg cctcgtgaag ttggtggacg tgacttacaa atcagcggtg gtgcaaccta
     5641 tattgactct gttattgttg aaaaaaagaa caaacaagta ctcatgtttg ctgatgtgat
     5701 gcctgctgga gtaagtttta gagaagcaac tagaaaagat tcaggttata aacaaattga
     5761 tggtaattat taccttaaat taaggaaaca aggtgatact gattacaatt atactattcg
     5821 tgagaatggt actgtatacg acgatcgtac caacagacca actgaatttt cagtagataa
     5881 aaatttcggt attaaacaaa atggtaatta tttgacggta gagcaatatt cggtttcatt
     5941 tgaaaataat aaaaagactg aatatcgtaa tgggactaaa gtccatatga atatcttcta
     6001 caaagatgca ttgtttaaag tagtgccaac gaactatatt gcatatattt ctagcaatga
     6061 tcatggcgaa tcttggtctg caccaacttt attacctcct ataatgggac ttaatcggaa
     6121 tgcgccatat ttaggtcctg gacgtggaat cattgaaagc tcaactggac gtattcttat
     6181 tccgtcttac actggtaaag agtctgcgtt catttatagt gacgataatg gagcatcttg
     6241 gaaagttaaa gtagtgccac ttccttctag ttggtcagca gaagcacaat ttgtagaatt
     6301 gagtccagga gtaattcaag catatatgcg tacaaataat ggtaaaattg catatttaac
     6361 aagtaaagac gcaggtacta cttggagtgc accggaatat ttgaaatttg tttcaaatcc
     6421 aagttatgga acacaattat caatcatcaa ttatagccaa ttgattgatg gtaaaaaggc
     6481 tgtcatttta agtactccaa actccacaaa tggtcgtaaa cacggacaaa tttggattgg
     6541 tctaattaat gatgataata caattgattg gcgttatcat cacgacgttg attatagtaa
     6601 ctatggatac tcatattcaa cattgacaga gttaccaaat catgaaattg gattgatgtt
     6661 tgaaaaattt gattcatggt ctcgtaatga acttcatatg aaaaatgttg taccatatat
     6721 aacatttaag attgaagatc tgaaaaagaa ttaaagctga aatttgaaaa tatataaaaa
     6781 gaggataaaa attatggtaa attacggtat tgttggagct ggatattttg gagctgattt
     6841 agctcgctca atgaacaaaa ttgaagatgc aaaagtggtt gcggtatttg acccaaatca
     6901 tggagaagaa gttgctcaag agttgggatc agatgtttgt gcaagtttag atgaacttgt
     6961 agcacgtgaa gatattgatt gtgtgatcgt agcttcacct agctaccttc accgtgaacc
     7021 agttgtgaaa gctgctcaac atggcaaaca cgtattttgt gaaaagccaa ttgcattgtc
     7081 ttatgaagat tgtaaagcca tggttgacgc atgtaaagaa aataatgtca tctttatggc
     7141 tggtcacatc atgaacttct ttaacggtgt acaccatgct aaagaattga ttactcaagg
     7201 taaaatcggt aaagttcttt attgccatgc cgctcgtaca ggttgggaag aacaacaacc
     7261 aactgtatca tggaagaaac ttcgttctca atctggagga catttgtacc accatattca
     7321 tgaattagat tgcattcagt tcatcatggg aggacttcct gaaaaagcga caatggtagg
     7381 aggcaatgta tatcataaag gtgaaaactt tggtgatgaa gatgatatgc tcattgtaaa
     7441 cctagaatac tctgatgatc gttatgctgt tttggaatat ggtaatgctt tccgttgggg
     7501 tgaacactac gtcttgattc aaggaactga aggagctatc aaacttgact tgttcaatac
     7561 tggaggtact cttcgtgtta aaggtgaagg agaatcacac ttcttagtac atgaaactca
     7621 agaggaagat gatgaccgta cagctatcta taccggtcgt ggtatggatg gagcaattgc
     7681 gtacggtaaa ccaggagtac gttgcccatt atggttacaa acatgtattg ataaagaaat
     7741 ggaatatcta catgacatca ttaaaggtgg agaaattaca gaagaatatg aaaaacttct
     7801 caatggtgta gctgctttag aatcaatcgc taccgctgat gcatgtactt tatcagttaa
     7861 agaagatcga aaagtaagtc tttcagaaat cacaaatgct taactattgt aaaacagaat
     7921 agtaaattct tgtcattata taatttctaa agttctgtga tacaactcat tgaataaaga
     7981 aatagagatg ggactgggat aatgcccagt cccatttttt atcaaaaagt aatgagatca
     8041 aaaatgtggg agtgatgaaa tgaagattat agggatcgat attggcggaa caacaattaa
     8101 ggcagattta tacgatgatt ttggaacgag tttaaatcag ttcaaagaga tagaaacaat
     8161 tattgactat gatttgggaa cgaaccagat attaaatcag gtctgtgatt taattggtga
     8221 gtatgtttta aattattcaa ttgatggtgt tgggatttcc actgctggag ttgttaatgc
     8281 taatacagga gaaatcatct atgcaggcta tacaatacca gggtatatcg gagtaaactt
     8341 tacttccgaa atagaaaaac gttttgggtt gtctactttt gttgaaaatg atgttaattg
     8401 tgctgcatta ggtgaattgt ggaagggaca agccaaagat aagaaaaatg tagtaatggt
     8461 tactattgga acaggtatag gaggcagtat tattgtcaac ggacaaattg ttaacggatt
     8521 taactatact gctggtgaag taggttatat tcctgtaggt aattcggatt ggcaaagtaa
     8581 agcctcaaca accgcattga ttcatttata tcaaaaaaag agcttgaaaa ctaatcaaac
     8641 tggacgtact ttcttcactg atttaagatc tggagataaa gttgctgaag aaacttttga
     8701 aatttttgta gaaaatctaa caaaaggttt attaacgatt tcttatctac ttaatccaga
     8761 aattctcata ttaggaggtg ggattctgga tagtaaggat attttgttac ctgaaattca
     8821 aagttcttta gctaaaaatg caatggataa taggttttta cctaaaaatc ttgtggcagc
     8881 tacattagga aatgaagctg gtcgtatagg agctgtaaaa aatttcttag atagaatttc
     8941 taataaatag tatgtaagat aaggaggtgt cacaatgact aactctgtat tttcgacaat
     9001 gcaagatatt gagaatgttg caaccgatat tataaaatca tatgataatg agatttatac
     9061 ttataaagct gtttcccaag aagaattgga aaaactagaa aaaagttatg atgaaaaaag
     9121 tcacgaagaa ttagtttcaa tagaaagcaa tttagaaatg aaacaacaga accttattga
     9181 tgaggttaat aaaacaatca aggaaaatga tgcaaatatt cagtatattt catcaagtag
     9241 gagaggagaa tttgtagaaa aaattattgg tagggtggta gaaaaatatg gccattagtc
     9301 agatgaaaag aatctctcta ctattttcta aaagtagtct tgatgatgtt ttaaaaacta
     9361 ttcaagaact agagtcagtg cagttccgtg atttaaaggt tcaggataac tggtcagaag
     9421 ctctagaaaa agatgaagtt gtatttccaa ctattcaaat ttttcatact tctaattcca
     9481 atcatggggt tattgaggga aatgatgcct tgacttattt gatgaatcaa caacaacatt
     9541 tagaagcaac tgtagagaaa ttacaagaat acctaccgaa agaaaacacg tttaaattat
     9601 tgcagcaacc tccgataact acctcttatg aagaattaga gaaatttggt aaagctaatg
     9661 ttgctgaggg tgttcttaaa aaagtgaatc atcaaattaa cagagttcat gaattagaaa
     9721 gacacattca aagtaataat gaggaaatag agcgattaat aaagtgggaa aaattagaaa
     9781 ttgttcctgc gaatttagaa caattttctt tctgtaaagg aaaagtcgga acaattccaa
     9841 ggactgaaga taatcgctta tacaatagtc ttttagaaaa caatattgaa gttcaagaaa
     9901 tattttctaa tgatagagag tacggtgttg ttgttttcta tcagtctagt tactctatag
     9961 attttgatga atacttattt gaaccatttg attattctag aaaggaatta ccgaagcagc
    10021 gagtagtaga tttagatcaa gaaaacatgc agttaataac tgaaaaagag aatattatcg
    10081 catcgttgca agattcaaag aaatatttga tagatttaca atggcaaata gactatattt
    10141 tatctatcta tgctcgtcaa atctctaaga ataacttttt gtgcactccg catctagttg
    10201 cattagaagg atggatagaa gaaactcgta ttttatattt tataaaagtt atggatgagc
    10261 attttggaca ttctatttat atttatgaat cggaaacatt gacggataat caagatgaaa
    10321 tacctatcaa attaacgaat cattctttaa ttgaaccatt tgaattattg acagaaatgt
    10381 atgctctgcc caaatattat gagaaagatc ctacacctgt attagcacca ttttacttta
    10441 cattttttgg aatgatggtt gctgatttag gctatggttt actattgttt ttaggaacaa
    10501 tgttagcatt aaaaattttt catctacctt cagcaactaa gagattttta aaattcttta
    10561 atatattagg ggtagccgtt gcaatttggg gtggaatcta tggctcattt tttggatatg
    10621 agttgccatt tcatctgata tctacaacct ctgatgtcat gactatatta gtagtgtcag
    10681 ttgtgtttgg gtttattaca gtatttgcag gtttgttagc ttcaggacta caaaaagtaa
    10741 gaatgaagaa atatgcagaa gcatataatt caggatttgc gtggtgtgtt attctgcttg
    10801 gcttgttatt tattgctgtt ggaatgttga tgcctgatat gagaccgtta tttgtattag
    10861 ggaaatgggt atctattttt aatgctgtgg ggattttgat tgtttctatt attcaaacca
    10921 aaagcttgtc aggtattgga gcaggattgt ttaatctata taacatttca tcttatatag
    10981 gtgatttagt tagtttcact cgattgatgg cattaggatt atctggagca agtatagcat
    11041 cagctttcaa tttaattgtt ggtttgtttc cgggaatatt ggctaaactg acaattggat
    11101 tagtattatt cattctttta catgcgatca atatttttct atcgttacta tcaggatatg
    11161 ttcatggagc acgtctgata tttgttgaat tttttggtaa gttttatgag ggtggaggaa
    11221 aaccatttca acctttgaag gcttctgaga aatatattaa ggttattaca aagaattaat
    11281 ggaggatata tataatggaa catttagcaa cttatttttc aacctatgga ggagctttct
    11341 tcgctgcatt gggaattgta ttggcggttg gattaagcgg tatggggtct gcttatggag
    11401 ttggtaaggc tgggcaatct gccgcagctt tactgaaaga acagcctgaa aagtttgcct
    11461 cagctttgat attgcaacta ttgcccggaa cacaaggatt atatggtttt gttattggaa
    11521 ttttgatttg gttgcaatta actccagaac ttcctttgga aaaaggcgtt gcttatttct
    11581 ttgtagctct tccaattgct attgtaggat acttttcagc taagcatcaa ggaaatgtag
    11641 cagtagcggg aatgcaaatc ttggctaaaa gaccaaaaga attcatgaag ggagcaattt
    11701 tagctgccat ggtagaaacc tatgcaattc ttgcttttgt cgtatcattc attttgaccc
    11761 ttcgtgtata agaaataaat ttgcaattca aaggaggtgt ctaaatgagc aatttagaaa
    11821 acttacgaga gtctgttatt gaacaagctc atgaaaaagg gcgtatgaaa ttattggatt
    11881 ccaaaaagaa gattgatgat gaatttgaaa tgcaaaagtc gctcattata aagaaaaaag
    11941 aagctgaaca tgaacgaaag ttaaaagaat tgcaacagaa atatcaaata atttttcaac
    12001 aattaaaaaa taaggaacgc caatcaacgt tagtatcaaa acagaaaata ttaaaagaac
    12061 tttttcaatc tgctttacta gaaatggaat cttggagtgc agataaagaa atggagttca
    12121 tctatcgaat tctggaacga tattcacaac aagaggtcat agtaaccttt ggggaacgga
    12181 ctttagctaa attcaatttg gaacaattag agaaattgaa attctctttt ccaaattatt
    12241 tatttagtga acaacctatc tcaaatgaat caggcttact tatttcaata ggtaaaattg
    12301 atgataacta tttgtataaa acattaattg gatcgatttc taaggaagaa agttcaagta
    12361 tcgcaaatca aatttttatc aattaaggat gaaattggtt aatcct
//
LOCUS       DAVKHZ010000062        12014 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000062 DAVKHZ010000000
VERSION     DAVKHZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.062"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(173..1804)
                     /locus_tag="VWW99_001617"
     CDS             complement(173..1804)
                     /locus_tag="VWW99_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9539432.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2097..7037
                     /locus_tag="VWW99_001618"
     CDS             2097..7037
                     /locus_tag="VWW99_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9539433.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEVLKQDTDGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELTTTKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNT
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7127..8323
                     /locus_tag="VWW99_001619"
     CDS             7127..8323
                     /locus_tag="VWW99_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9539434.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8459..8986
                     /locus_tag="VWW99_001620"
     CDS             8459..8986
                     /locus_tag="VWW99_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9539435.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9063..9419
                     /gene="glnR"
                     /locus_tag="VWW99_001621"
     CDS             9063..9419
                     /gene="glnR"
                     /locus_tag="VWW99_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9539436.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9456..10802
                     /gene="glnA"
                     /locus_tag="VWW99_001622"
     CDS             9456..10802
                     /gene="glnA"
                     /locus_tag="VWW99_001622"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9539437.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10892..11011)
                     /locus_tag="VWW99_001623"
     CDS             complement(10892..11011)
                     /locus_tag="VWW99_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9539438.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKTNSQKSQEDFSKNK"
     gene            11499..12014
                     /locus_tag="VWW99_001624"
                     /pseudo
     CDS             11499..12014
                     /locus_tag="VWW99_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3858 a         2196 c         2575 g         3385 t
ORIGIN      
        1 aatcgttcag gacagtcaaa tcgatttcta acaatgtttt agaaatagag gtgtactatt
       61 ctagtttcaa tatattatat atctgcagaa tactctttca gtttcattca gcctagtctt
      121 gcttaggaaa aagtaagaag ccatccaatt atgatggata gcttcttggt tattagattt
      181 gttctgcaaa aaccttttca gcaaggttca tagcgtggtc tgatacacga gtgtagtgtg
      241 agatgatgtc gataaagttg actccagctt gtgttgaaca ttcgcctttg ttgaggcgtt
      301 tgatgtgggt ttttctgaga acacgttcta tcttattgat tgcttcatga cgttctacaa
      361 gactgcgtgc tttttcaata tcattgtttt ccacactatc cagagcatct ttgataaagt
      421 cactagtttg gcggtaaact tcctctaatt ctttcaaggc ggcatcagaa aattcaacat
      481 ttttccgttg aagatagtca gtcagattga gtagagcctc cgtgtggtct ccaatccgtt
      541 ccaaatcacg ggaggaatca aggatattgg taagcacttc actttctttt tggctgagag
      601 cttcgcttga aagggcaatg agataacgtg ttaattgctc atcgatggtg ttaattgctt
      661 cttcggtttt atgccctttt tcagcaactt tttcatccaa gtcaatgatg tacttatagg
      721 aaaggtcaaa ggctttagca gcgtagtttc ctaagtgcaa gagctctttc ttagcatttc
      781 ctagagcgat agatggggcc tgtttgatga aatgttcatc aagatataag ggttcgtatt
      841 tgacaacctc gtcctctcca ggaataatct tggttacaaa gtaagccaga gctccgataa
      901 atggaaattg gacaatggtg ttggtaatat taaaggttcc gtgagcaaag gcgatggtca
      961 tttccggtgc tagatttagc gtagcttcaa accaatgaat caggacagta aaaggaacta
     1021 gaaaaataac gcagacgact gttccgataa cgttgaaggc aacatgagct cctgctaccc
     1081 gtttagctgc aatattagcc cctaaagagg caatgatggc tgtaatggtt gtcccgatat
     1141 tgtcaccaaa tagaactggc aaagctccct gtaggtcaat tagattgccg gcgtagaggt
     1201 tttgtaaaat cccaatggta gccgaagaag cttgaattag caaggtcaag ccagtaccga
     1261 caaagacacc caaaacagga ttcttactta gctcaatcat atagtcctta aagacctgta
     1321 aatccttgag tggagccatt gcgccgctca tgagattgag ggcaaaaaag ataccaccga
     1381 taccaaagag gatgcgtccg atattattga ctgtccgatt tttcgtaaaa aagagacaga
     1441 cggcaccgat aaagagcata ggtagggcat agttacctaa tttaaaaccg atgagaaagg
     1501 atgtgacagt tgtcccaata ttagcaccca tgacaatccc gatagcctga cgtaaggtta
     1561 agagaccggc actgaccagg ccgactgtaa taactgttac accagaacta gactgaatta
     1621 gagcagtcat cccaatacca accagaactc caaagaaagg attactagta tatttgtcaa
     1681 tgtaaaaacg aaggcgatct ccagcagctt gttgcaaacc gtctcccatg gtcttgatgc
     1741 tatataagaa tagccccaga ccacctaaaa agtgaaataa aatttcctgc caattaatgg
     1801 acatttcttt ttcctccgaa aaataatcgc ggaatatctc ctattctatt ttaaaggata
     1861 aaagtaaatc taacaagtgt taatgtcaag tttgtaaaag aaaattataa ttgataagtg
     1921 tattattttt aagagttttt aaccttattt tagatttgtg caaacagtta ttgttttttg
     1981 tctaaaaatg ttttaggata aaaatgtgat tatcatggtt ctagagtttg ataaaaaact
     2041 acagaatata aagcgctttc ttgaaaacaa caaaatcaat cttttaggag gagaaaatga
     2101 agaatccatt ttttgaaaga cgttgtcgtt acagtattcg taagttatca gtaggagcct
     2161 gctcgctgat gattggtgct gttttatttg ctggtccagc cttggctgaa gaaactgcag
     2221 ttcctgaaaa tagcggagct aatacagagc ttgtttcagg agagagtgag cattcgacca
     2281 atgaagctga taagcagaat gaaggggaac atgctagaga aaacaagcta gaaaaggcag
     2341 aaggagtagc gacagcatct gaaactgcag aagcagctag cgcagctaaa ccagaggaaa
     2401 aagcaagtga ggtggttgca gaaacaccat ctgcagaagc aaaacctaag tctgacaagg
     2461 aaacagaagc aaagcccgaa gcaactaacc aaggggatga gtctaagcca gcagccgaag
     2521 ctaataagac tgaaaaagaa gtccagccag atgtccctaa aaatacagaa aaaacattaa
     2581 aaccaaagga aatcaaattt aattcttggg aagaattgtt aaaatgggaa ccaggtgctc
     2641 gtgaagacga tgctattaac cgcggatctg ttgtcctcgc ttcacgtcgg acaggtcatt
     2701 tagtcaatga aaaagctagc aaggaagcaa aagttcaagc cttatcaaac accaattcta
     2761 aagcaaaaga ccatgcttct gttggtggag aagagttcaa ggcctatgct tttgactatt
     2821 ggcaatatct agattcaatg gtcttctggg aaggtctcgt accaactcct gacgttattg
     2881 atgcaggtca ccgtaacggg gttcctgtat acggtacact cttcttcaac tggtctaata
     2941 gtattgcaga tcaagaaaga tttgctgaag ttttgaagca agacacagat ggtagcttcc
     3001 caattgcccg taaattggta gacatggcca agtattatgg ctatgatggc tatttcatca
     3061 accaagaaac aactggagat ttggttaaac ctcttggaga aaagatgcgc cagtttatgc
     3121 tctatagcaa ggaatatgct gctaaggtaa accatccaat caagtattct tggtatgatg
     3181 ccatgaccta taactatgga cgttaccatc aagatggttt gggagaatac aactaccaat
     3241 tcatgcaacc agaaggagat aaggttccgg cagataactt ctttgctaac tttaactggg
     3301 ataaggctaa aaatgattac actattgcaa ctgccaactg gattggtcgt aatccttatg
     3361 atgtatttgc aggtttggaa ttgcaacagg gtggttccta caagacaaag gttaagtgga
     3421 atgacatttt agacgaaaat gggaaattgc gcctttctct tggtttattt gccccagata
     3481 ccattacaag tttaggaaaa actggtgaag attatcataa aaatgaagat atcttcttta
     3541 caggttatca aggagaccct actggccaaa aaccaggtga caaagattgg tatggcattg
     3601 ctaacctagt tgcggaccgt acgccagcgg taggtaatac ttttactact tcttttaata
     3661 caggtcatgg taaaaaatgg ttcgtagatg gtaaggtttc taaggattct gagtggaatt
     3721 atcgttcagt atcaggtgtt cttccaacat ggcgctggtg gcagacttca acaggggaaa
     3781 aacttcgtgc agaatatgat tttacagatg cctataatgg cggaaattcc cttaaattct
     3841 ctggtgatgt agctggtaag acagatcagg atgtgagact ttattctact aagttagaag
     3901 taactgagaa gaccaaactt cgtgttgccc acaagggagg aaaaggttct aaagtttata
     3961 tggcattctc tacaactcca gactacaaat tcgatgatgc agatgcatgg aaagagctaa
     4021 ccctttctga caactggaca aatgaagaat ttgatctcag ctcactagcg ggtaaaacca
     4081 tctatgcagt caaactattt ttcgagcatg aaggtgctgt aaaagattat cagttcaacc
     4141 tcggacaatt aactatctcg gacaatcacc aagagccaca atcgccgaca agcttttctg
     4201 tagtgaaaca atctcttaaa aatgcccaag aagcggaagc agttgtgcaa tttaaaggca
     4261 acaaggatgc agatttctat gaagtttatg aaaaagatgg agacagctgg aaattactaa
     4321 ctggctcatc ttctacaact atttatctac caaaagttag ccgctcagca agtgctcagg
     4381 gtacaactca agaactgaag gttgtagcag tcggtaaaaa tggagttcgt tcagaagctg
     4441 caaccacaac ctttgattgg ggtatgactg taaaagatac cagcctacca aaaccactag
     4501 ctgaaaatat cgttccaggt gcaacagtta ttgatagtac tttccctaag actgaaggtg
     4561 gagaaggtat tgaaggtatg ttgaacggta ccattactag cttgtcagat aaatggtctt
     4621 cagctcagtt gagtggtagt gtggatattc gtttgaccaa gccacgtacc gttgttagat
     4681 gggtcatgga tcatgcaggg gctggtggtg agtctgttaa cgatggcttg atgaacacca
     4741 aagactttga cctttattat aaagatgcag atggtgagtg gaagctagct aaggaagtcc
     4801 gtggtaacaa agcacacgtg acagatatca ctcttgataa accaatcact gctcaggact
     4861 ggcgcttgaa tgttgtcact tctgacaatg gaactccatg gaaggctatt cgtatctata
     4921 actggaaaat gtatgaaaag cttgatactg agagtgtcaa tattccgatg gccaaggctg
     4981 cagcccgttc tctaggcaat aacaaggtac aagttggctt tgcagatgta ccggctggag
     5041 caactattac cgtttatgat aatccaaatt ctcaaactcc gctcgcaacc ttgaagagcg
     5101 aagttggagg agacctagca agtgcaccat tggatttgac aaatcaatct ggtcttcttt
     5161 attatcgtac ccagttgcca ggcaaggaaa ttagtaatgt cctagcagtt tccgttccaa
     5221 aagatgacag aagaatcaag tcagtcagcc tagaaacagg acctaagaaa acaagctacg
     5281 ccgaagggga ggatttagac cttagaggtg gtgttcttcg agttcagtat gaaggaggaa
     5341 ctgaggacga actcattcgc ctaactcacg caggtgtatc agtatcaggt tttgatacgc
     5401 atcataaggg agaacagaat cttactctcc aatatttggg acagccagta aatgctaatt
     5461 tgtcagtgac tgtcactggc caagatgaag caagtccgaa aactattttg ggaattgaag
     5521 taagtcagga accgaaaaaa gattacctag ttggtgatag cttagacttg tctgaaggac
     5581 gctttgcagt ggcttatagc aatgacacca tggaagaaca ttcctttact gatgagggag
     5641 ttgaaatttc tggttacgat gctcaaaaga ctggtcgtca aaccttgacg cttcgttacc
     5701 aaggtcatga agtcaacttt gatgttttgg tatctccaaa agcagcattg aacgatgagt
     5761 acctcaaaca aaaattagca gaagttgaag ctgctaagaa caaggtggtc tataactttg
     5821 cttcaccaga agtaaaagaa gccttcttga aagcaattga agcggccgaa caagtgttga
     5881 aagaccatga aactagcacc caagatcaag tcaatgaccg acttaataaa ttgacagaag
     5941 ctcataaagc tctgaatggt caagagaaat ttacggaaga aaagacagag cttgatcgct
     6001 taacaggtga ggttcaagaa ctcttggctg ccaaaccaaa ccatccttca ggttctgccc
     6061 tagctccgct tcttgagaaa aacaaggcct tggttgaaaa agtagatttg agtccagaag
     6121 agcttacaac aacgaaacag agtctaaaag atctggttgc tttattgaaa gaagacaagc
     6181 cagcagtctt ttctgatagt aaaacaggtg ttgaagtaca cttctcaaat aaagagaaga
     6241 ctgtcatcaa gggtttgaaa gtagagcgtg ttcaagcaag tgctgaagag aagaaatact
     6301 ttgctggaga agatgctcat gtctttgaaa tagaaggttt ggatgaaaaa ggtcaagatg
     6361 ttgatctctc ttacgcttct attgtgaaaa tcccaattga aaaagataag aaagttaaga
     6421 aagtattttt cttacctgaa ggcaaagagg cagtagaatt ggcttttgaa caaacggata
     6481 gtcatgttat ctttacagca ccacacttta ctcattatgc ctttgtttat gaatctgctg
     6541 aaaaaccaca acctgctaaa ccagcaccac aaaacacagt ccttccaaaa cctacttatc
     6601 aaccggcttc tgatcaacaa aaggctccta aattggaagt tcaagaggaa aaggttgcct
     6661 ttcatcgtca agagcatgaa aatactgaga tgctagttgg ggaacaacga gtcatcatac
     6721 agggacgaga tggactgtta agacatgtct ttgaagttga tgaaaacggt cagcgtcgtc
     6781 ttcgttcaac agaagtcatc caagaagcga ttccagaaat tgttgaaatt ggaacaaaag
     6841 taaaaacagt accagcagta gtagctacac aggaaaaacc agctcaaaat acagcagtta
     6901 aatcagaaga agcaagcaaa caattgccaa atacaggaac agctgatgct aatgaagccc
     6961 taatagcagg cttagccagc cttggtcttg ctagtttagc cttgaccttg agacggaaaa
     7021 gagaagataa agattaaata tcgaaaaatc ttgtgaaatc tttccttata tttccaaagt
     7081 gtgatataat agtttcgaat aaaataaata aaggagtttt tgtaacatgg caaaacttac
     7141 tgttaaagac gttgacttga aaggtaaaaa agtcctcgtt cgtgttgact tcaacgtacc
     7201 attgaaagat ggcgtaatca ctaacgataa ccgtatcaca gcagctcttc caactattaa
     7261 gtacatcatc gaacaaggtg gacgtgcaat tcttttctct caccttggac gtgtgaaaga
     7321 agaagctgat aaagctggta aatcacttgc tcctgtagca gctgacttgg cagcaaaact
     7381 tggtcaagat gttgttttcc caggtgtcac tcgtggtgct gaattggaag cggcaatcaa
     7441 cgctcttgaa gatggacaag ttctcttggt tgaaaacact cgttacgaag atgttgacgg
     7501 caagaaagaa tctaaaaacg atcctgaact tggtaaatac tgggcatcac ttggagatgg
     7561 tatcttcgta aacgatgcat tcggtacagc tcaccgtgca cacgcatcta acgttggtat
     7621 ctcagcaaac gttgaaaaag cagttgctgg tttccttctt gaaaacgaaa ttgcctacat
     7681 ccaagaagca gttgaaactc cagaacgtcc attcgtggct atccttggtg gttcaaaagt
     7741 ttcagacaag atcggtgtta tcgaaaactt gcttgaaaaa gctgataaag tccttatcgg
     7801 tggtgggatg acttacacat tctacaaagc acaaggtatc gaaatcggta actcacttgt
     7861 agaagaagac aaattggatg ttgcgaaagc tcttcttgaa aaagcaaatg gtaaattgat
     7921 cttgccagtt gactcaaaag aagctaacgc atttgctggt tacactgaag tgcgtgacac
     7981 tgaaggtgaa gcagtttctg aaggcttcct tggtcttgac atcggtccaa aatctatcgc
     8041 caaatttgac gaagctttga ctggtgccaa aacagttgta tggaacggac ctatgggtgt
     8101 atttgaaaac ccagatttcc aagctggtac aatcggtgtg atggacgcta tcgtgaaaca
     8161 accaggagtt aaatcaatca tcggtggtgg tgactcagct gccgcagcga ttaaccttgg
     8221 ccgtgcagac aagttctcat ggattagtac gggtggtgga gcatcaatgg aacttcttga
     8281 aggtaaggtt cttccaggac ttgcagcctt gacagaaaaa taagatttta taaacaaatc
     8341 aaagaagaga gggatgagag ttcctctttt cttttgctta aaataaaaac gcttcctctc
     8401 aactattact cataaaaatc accgatttat gataaaatgg aaatagaaag ttgagattat
     8461 gagttatttt aaaaaatata aattcgataa atcccagttc aaacttggta tgcgaacctt
     8521 taaaacaggt attgctgttt ttctagttct cttgattttt ggcttttttg gctggaaagg
     8581 tcttcaaatt ggtgctttga cagccgtttt tagcctgagg gagagttttg atgagagtgt
     8641 tcattttggg acttcgcgta ttctaggaaa tagtatcggt ggactctatg ccttggtctt
     8701 cttcttatta aatacctttt tccacgaagc cttttgggtg accttggtag ttgttccaat
     8761 ctgcaccatg ttaaccatta tgacaaatgt agccatgaat aacaaagcag gggttattgg
     8821 tggtgtagca gctatgttaa tcattaccct atcaattcca agtggtgaga caatttcgta
     8881 cgtgtttgtg cgtgtatcag aaacgtttat gggagttttt gtcgcaatta tcgtaaatta
     8941 cgatattgat cgtattcgtc tctttttaga gaaaaaagaa aaataatgtt acattttata
     9001 acattatcaa ttgacgtttg tcttttttta gactataata gacagaaaga aggaaattgt
     9061 aaatgaagga aaaagaattt cgccgaaata tggctgtttt tcctattggc agtgttatga
     9121 agttgaccga tctatcggcg cgtcagattc gttattatga agatcaagag ttgatcaagc
     9181 ccgatcgaaa cgaaggaaat cgtcgcatgt attccttgaa tgacatggat cgtctgcttg
     9241 aaatcaaaga ttatatctct gaaggttata atatcgctgc cattaagaaa aaatatgctg
     9301 aacgtgaagc gaaatccaag aaagcggtta gtcagactga agtacgtcgt gcacttcaca
     9361 atgaactcct ccaacagggg cgttttgctt cagtacagtc accttttggt cgcggttagg
     9421 caaccgcaag tagtcataga ttaaggagaa aacttatgcc aatcacagct gcagatattc
     9481 gtcgtgaagt caaggaaaaa aatgttacct ttattcgtct tatgttctca gatattttgg
     9541 gaaccatgaa aaacgtcgaa attcctgcta cagatgaaca gttagataag gtcttgtcga
     9601 acaaggttat gtttgatgga tcttctattg aaggttttgt acgtatcaat gagtcggata
     9661 tgtacttgta cccggacttg gatacatgga cagtcttccc ttggggagat gaaaatggaa
     9721 gtgttgcagg tctgatctgt gatgtctata caacagaagg tgaaccattt gcgggtgacc
     9781 ctcgtggtaa tttgaaacga gctcttcgtc acatggaaga agttggattc aaatccttca
     9841 accttggtcc agagccagaa ttcttcctat ttaagttgga tgaaaatggg gacccaacac
     9901 ttgaagtgaa tgacaagggt ggctactttg acttggcacc tactgacctt gcggacaaca
     9961 cacgtcgtga gattgtgaat gtcttgacca aaatgggatt tgaagtagaa gcgagtcacc
    10021 acgaggttgc ggttggacag catgagattg actttaagta cgatgaagtt ctccgtgctt
    10081 gtgataagat tcaaatcttt aaacttgttg ttaaaaccat tgctcgcaaa cacggacttt
    10141 acgcaacatt tatggcgaag ccaaaatttg gtattgctgg atcaggtatg cactgtaata
    10201 tgtccttgtt tgatgcagaa ggaaataacg ccttctttga tccaaatgat ccaaaaggaa
    10261 tgcagttgtc agaaacagct taccatttcc taggcggttt gatcaagcat gcttacaact
    10321 atactgccat catgaaccca acagttaact catacaaacg tttggttcca ggttatgaag
    10381 cgcctgttta cattgcttgg gctggtcgta accgttcgcc acttgtgcgc gtacctgctt
    10441 cacgtggtat gggaactcgt cttgagttgc gttcagtgga tccaatggcg aacccttaca
    10501 ttgctatggc tgttcttttg gaagttggtt tgtatggtat tgaaaataaa atcgaagcac
    10561 cagctcctat cgaagaaaat atctacatca tgacagcaga agagcgcaag gaagctggta
    10621 ttacagacct tccatcaact cttcacaacg ctttgaaagc tttgacagaa gatgaagtgg
    10681 ttaaagctgc tctcggagat cacatctaca ctagcttcct tgaagccaaa cgaatcgaat
    10741 gggcaagtta tgcaaccttc gtttcacaat gggaaattga taattattta gacctttact
    10801 aatatagaag aaagattgcc tgtgggtaaa cagtcacaca gcagtctaag ccatcaaata
    10861 cctataaccc ttaaaatagc gatttcggtc gctatttatt tttcgaaaaa tcctcttgac
    10921 tcttttgcga attagtttta tcatcagacg tgtagaagaa aattaaggag tcactatgga
    10981 aaaattcaat ttcaaaaaca atatcgggca agaaaacaag ttacttcaaa tcgaaatcta
    11041 caagtttact aacttttgca agcttcaaaa ttacacatct gtaaatattt tttcgaaaga
    11101 tatatttgaa gccatagtaa attgagacta gaatagtaca ctacatctgt taaaaatttc
    11161 tagaaactaa tttaactttc ctaatcgatt tgtttacatc ttatttcaat ctactatatt
    11221 atctcaataa atgatgatgg cgactgtttt gtttgtgctt gtcgattttt gaatcctgat
    11281 cgagcggaag aatatttctg ccgaggttgc caaggggaaa atactgaaga tattgaattt
    11341 tatgatgaac aattacaggc agaaaaggtg gaggttttga atattcgatt ggaagtaaaa
    11401 aattgaatta tctacaatct catcaacaat aaacctattt caaccccatt atctataggc
    11461 gtatttttac gcctttttat atacttgaag cacataagaa acattatggg aagtttgctg
    11521 atggaagcac tcaagaaatt gatgttcctt atgatattcc tgatacttgg gagtgggtga
    11581 ggataaaatc aatttattgg aattttgggc aaaataagcc agagaaatcc tttaggtata
    11641 tagatacgtc tagtattgat agaaaaaaga atataatcaa ctacaaaaat ctacaatatc
    11701 tttcacctga acaagcgcct tcccgtgcta gaaaattagt ttcgcagaat agtgtcttat
    11761 tttcaacagt tagaccatat ctaaaaaata ttgctgtagt tagagaactt aaagagtatt
    11821 tgatagctag tacagcattt attgttttgg atactttact taacgaaaca tatttgaaat
    11881 attatttatt atcagataat tttatcaatc gtgttaataa taaatctact ggaacaagtt
    11941 atcctgcaat caatgattat aattttaatc tattattaat tgctctcccc ccactatccg
    12001 aacaacaacg aata
//
LOCUS       DAVKHZ010000063        11889 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000063 DAVKHZ010000000
VERSION     DAVKHZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.063"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            111..764
                     /locus_tag="VWW99_001625"
     CDS             111..764
                     /locus_tag="VWW99_001625"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9539439.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANTFLKETGQEPIDWLR"
     gene            774..1238
                     /locus_tag="VWW99_001626"
     CDS             774..1238
                     /locus_tag="VWW99_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9539440.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLGDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1251..2519
                     /locus_tag="VWW99_001627"
     CDS             1251..2519
                     /locus_tag="VWW99_001627"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9539441.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATRAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2533..2793
                     /locus_tag="VWW99_001628"
     CDS             2533..2793
                     /locus_tag="VWW99_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539442.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2804..4165
                     /gene="pdrM"
                     /locus_tag="VWW99_001629"
     CDS             2804..4165
                     /gene="pdrM"
                     /locus_tag="VWW99_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9539443.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNYIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4319..5287
                     /locus_tag="VWW99_001630"
     CDS             4319..5287
                     /locus_tag="VWW99_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9539444.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEKIQAQVKEAVEASVKFAEESPFPPLESAFEDIYTD"
     gene            5303..6295
                     /locus_tag="VWW99_001631"
     CDS             5303..6295
                     /locus_tag="VWW99_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9539445.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6513..7556
                     /locus_tag="VWW99_001632"
     CDS             6513..7556
                     /locus_tag="VWW99_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9539446.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLATSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7620..9305
                     /gene="lpdA"
                     /locus_tag="VWW99_001633"
     CDS             7620..9305
                     /gene="lpdA"
                     /locus_tag="VWW99_001633"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9539447.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9369..10358
                     /locus_tag="VWW99_001634"
     CDS             9369..10358
                     /locus_tag="VWW99_001634"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9539448.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     HHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10431..11501)
                     /gene="xerS"
                     /locus_tag="VWW99_001635"
     CDS             complement(10431..11501)
                     /gene="xerS"
                     /locus_tag="VWW99_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9539449.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGCKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3525 a         2224 c         2659 g         3481 t
ORIGIN      
        1 tattcaaatc gtatatagtc gatttattaa atctttagat aaactgtaaa aagtggtttc
       61 catagccact ttttgctata atagaggcag taaaaatgaa ggagtcggct atggaacact
      121 cgtcttggca tgctttgatt aaggcgcaat tacctgaggg ttatttcggg aaaatcaatc
      181 agtttatgga gcaggtctat tctcagggga ttatttatcc acccaaggaa aaggtttttc
      241 aggctctctt gacaacactg cttgaagaag ttaaggtggt aattctaggg caagacccct
      301 atcacggacc aggtcaagcg cagggcttga gtttttctgt acctgactct atcccagctc
      361 caccatcctt gcaaaatatc ttgaaagaat tgtcagatga tatcggagtt aagaaatctc
      421 atgatttgac agcttgggct gagcaaggag tcttgcttct taatgcttgt ttgactgttc
      481 ctgctggaca ggccaatggt catgctggtc aaatctggga gccttttact gatgctgtga
      541 ttcaggtggt caatcatcta gatagaccag tcgtttttgt actctgggga gcttatgcac
      601 gtaagaagaa ggccttagtt accaatcctc atcacttgat tatcgaatca gcccatccaa
      661 gtcctttgtc ggtttataga ggattttggg gttccaagcc tttttccaag gccaatacat
      721 tcttaaaaga gacaggacaa gagccaatcg attggcttag ataaggagaa aatatgcctc
      781 agttagcgac gatttgctac attgataatg ggaaagaact gctcatgctc catcgtaata
      841 agaaacccaa tgatgtccat gaagggaaat ggattggtgt gggtggtaag ctagagagag
      901 gagaaacgcc ccaggaatgc gcggtgcgtg aaatccttga agaaacaggg ctcaaagcca
      961 agccagttct aaaaggtgtc atcacttttc ctgaatttac accagattta gactggtaca
     1021 cctatgtttt taaggtgacg gagtttgagg gcgacttgat tgactgcaat gaggggacgc
     1081 tagaatgggt tccctatgat gaggttttga gcaagccgac ttgggaaggt gaccacacct
     1141 ttgttgagtg gcttttaggg gataaaccct tcttttcagc caagtttgtt tatgatgggg
     1201 ataaattgtt ggatacccaa gttgatttct atgaataaag gagaaagcag atgctactaa
     1261 tcaaaaatgg tcgtgtaatg gatcctaagt ctggtttgga tcaagtttgt gatgtcttag
     1321 ttcaagatgg gaaaattatc aaaattgcgc ctgagatcac ggaagaagga gcagaaacga
     1381 ttgatgctac tggtcttgtg gttgctcctg gtttggtcga tattcatgtg catttccgtg
     1441 aacctggtca aacacataaa gaagacattc atactggtgc cctggcagcc gctgcaggtg
     1501 gttttactac tgtcgtcatg atggctaata ccagtccaac catttcagac gtggagactt
     1561 tgcaagcagt tctccagtca gctgccaaag agaagattaa tgtcaagaca gttgcgacca
     1621 ttactaaaaa ctttaatggt aaaaacttga ctgactttaa ggcgctctta gaagctggtg
     1681 cggttggttt ctctgatgac ggtattccgc ttgagagcag taagattgtc aaggaagcca
     1741 tggaggaagc caaaaaactc aataccttta tcagccttca tgaggaagat ccaggtttga
     1801 acggtgttct tggctttaat gaaaatattg ctagagaaca tttccatatc tgcggtgcta
     1861 ctggggtggc tgagtacgct atgatggcgc gtgatgtcat gattgcctat gcaactagag
     1921 cccatgttca catccagcat ttgtctaagg aagaaagtgt taaagtagtg gagtttgctc
     1981 aggggttagg tgcagaagtc acagcagaag tagcaccaca gcatttctct aagacagaag
     2041 cacttctttt aacacaaggt agcaatgcta agatgaatcc accgcttcgt ttggaatcag
     2101 accgtcgtgc cgttatcgaa ggtctcaaat caggtgtcat cacagttatt gcgactgacc
     2161 acgcgcctca tcatgtagat gaaaaaaatg ttgaagatat taccaaagcg ccatctggta
     2221 tgactggctt agaaacatcc ctgtctctcg gcttgaccta tttagtagaa gctggtgagt
     2281 tgagcttgat ggaattactt gaaaaaatga catacaaccc agccaagctt tacaactttg
     2341 aagcaggtta cttggctgag aatggtccag cagatatcac tatttttgat gccaaggctg
     2401 accgccttgt ggactcccat tttgcttcca aagcagctaa ttcaccattc atcggtgaaa
     2461 ccttaaaagg gcaggttaaa tataccatct gtaagggaca aatcgtctat caagcttgat
     2521 aggagttaga atatgaatta ttttcgaaaa cgtagggaga gacaagcgaa atctaatagt
     2581 ggaatttact gtcccgcagc taatcgttcc aaaattcaat atgaaactaa agaaaaagct
     2641 gaccatgcgg ttcaatatga taacggaggt ttagttagaa gctattattg tcgtacatgt
     2701 gcgtgttggc atactacttc aaaatctaat aaacctccgc taagtttgaa aaactatggt
     2761 ctgaaactct ttggtttaga taaatcagaa tagaaagaga agtatgtata agacaaagtg
     2821 tttacgagag aagttagtat tatttttaaa aattttcttc ccaatcctga tctaccaatt
     2881 tgccaattat tctgcctctt ttgttgatac tgcaatgaca ggtcaataca acactatgga
     2941 cttggctggt gtatctatgg caaccagtat ctggaatcct ttctttacat ttctaacagg
     3001 gattgtgtca gccttggtgc ctatcattgg tcaccatctt ggtcgaggca aaaaggaaga
     3061 agttgcgtct gatttttacc aatttattta tttggccttg ggcctatctg tggtcttgct
     3121 ggggatggta cttttcttgg caccaacaat cttgaattat attgggttag aagcagcagt
     3181 agcggcagta gcggttcgct atctttggtt tttatctatc gggattatcc ccttgttgct
     3241 ctttagcgtc attcgttcct tgctggattc gctgggcttg accaaactgt ccatgtacct
     3301 catgcttttg ttactccctc tcaatagcgg atttaactat ctcttgattt acggtgcctt
     3361 tggtgttcca gaactgggag gggctggtgc tggtttagga acatccttgg cctactgggt
     3421 cttgcttggg atttctgttc tggttttatt taaacaggag aagctcaaag ccttacacct
     3481 tgagaaacga attccactta atatggataa cattaaggaa ggcgttcgtt taggtctgcc
     3541 tattggggga actgtcttcg cggaagtggc tatcttttca gtggttggct tgattatggc
     3601 taagttttcg tccttgatta tagctagtca ccagtcagct atgaactttt caagtcttat
     3661 gtacgccttt cctatgagta tctcatcggc tatggctatt gtcgtttcct atgaagtggg
     3721 agccaagcga tttgatgatg cgaaaaccta tattggtcta ggaagatgga ctgccctcat
     3781 ttttgcggcc ttcaccttaa ccttccttta catttttagg ggaaatgtgg ccagtcttta
     3841 tggtaacgac ccaaaattta tcgatttgac agcgcgtttt ttaacttata gtcttttctt
     3901 ccagttagca gatacctttg cggcgccgct tcagggaatt ttgcgggggt ataaggatac
     3961 agttattcct ttttaccttg gtttgcttgg ttattggggc gtagcaatcc ctgtggctac
     4021 gctatttgat tccctaacag attttggagc ttattcttac tggatcggct tgattattag
     4081 tttgattgtg agtggggcgc tctaccgttg gcgtttaact gtgattatga agagatttga
     4141 atctttagca aaatccaaac gctaaaaagt ttgtgaaaaa atatccttga caagaaaatc
     4201 ccttgttttc acggggtttt cttttttatt ttgtgaaatt tcttttagaa aaactttgac
     4261 agtatttgcc aaaagcggta aaatagtaag gtaagaagaa agttagaaag gcaagaagat
     4321 gtcaacttta gataaaaatc ttttgctaga aatgttccgt aagatggaag aaatccgtcg
     4381 catggactta aaaattgcac aattagtaaa gaaagggaaa gtgccaggaa tgacgcactt
     4441 ttctgttggt gaagaggcag ctaacgtggg ggctatgtta gctctcaatc cagatgatct
     4501 gattacctca aaccaccgtg gacacgggca agctattgct aaagggattg acctcaacgg
     4561 aatgatggct gaaatccttg gtaaatacac tggaacctgt aaagggaaag gtggatctat
     4621 gcatatcgct gaccttgatg ctgggaacct tggtgccaat ggtatcgtag gtggtggtat
     4681 ggggatcgct gtcggtgcag ccctcagtca gcaaatgcaa aataccggta aaatcgttgt
     4741 ctgcttcttt ggagatggtg cgaccaatga aggtgttttc cacgaagcag tgaacatggc
     4801 ttctatctgg aacctgccag tcattttcta ttgcattaac aacggttacg gtatctctgc
     4861 ggatatcaag aaaatgacca atatagaaca tatccatcaa cgtagcgccg cttatggaat
     4921 tcctggaatg ttcatcgaag acggtaacaa tgtcatcgat gtctatgaag gatttcagaa
     4981 agctgtagac catgttcgca gtggcaatgg tccagtcttg attgaaagtg taacttatcg
     5041 ctggctcggt cactcatcat ctgaccctgg taaatatcgt acgcgtgaag aagtggaatt
     5101 gtggaaacaa aaagatccaa tcgaaaacct ccgcaattac cttattgaaa ataacattgc
     5161 aagtgccgaa gaattggaaa aaatccaagc gcaagtaaag gaagcagtag aagcttctgt
     5221 taaatttgca gaggaaagcc cattcccacc gcttgaatca gcctttgaag atatttacac
     5281 agactaagga gaaagagata aaatggaaac aaaaacaatg tccttccgtg acaccattat
     5341 ccttgctatg tctgaggaaa tgcgtcgcga tgaaaatgtg ttcttgatgg gagaagatgt
     5401 cggtgtcttc ggaggagact tcggtacttc tgttggaatg cttgaagaat ttggtccaga
     5461 acgtgtccgt gactgtccga tttctgaagc tgccatctct ggagcagcag caggagcagc
     5521 catgacagga cttcgtccaa tcgtcgatat gaccttcatg gacttctctg ttattgccat
     5581 ggacaatatc gtcaaccaag ctgctaaaac acgttacatg tttggtggta aaggtcaggt
     5641 tccaatgact gttcgttgtg cagctggtaa cggagttggc tctgccgccc agcactcaca
     5701 atctctagag tcttggttta ctcacattcc aggacttaag gttgtggcac ctggaacacc
     5761 tgcggacatg aaaggcctgc ttaagtcttc tatccgtgat aacaacccag ttattatact
     5821 tgagtacaag tcagaattta accaaaaagg ggaagtgcca gttgatccag actacacaat
     5881 tccacttggg gttggtgaaa tcaaacgcca aggaacggat gtaacagttg ttacttatgg
     5941 aaaaatgctt cgccgtgtgg ttcaagctgc tgaagaatta gcagaagaag gaatttcagt
     6001 tgaaattgtt gacccacgta cccttgttcc gcttgataag gatatcatca taaactcagt
     6061 gaagaagact ggtaaggttg ttctggtcaa cgatgcccac aaaacaagtg gctatatcgg
     6121 tgaaatttca gctattattt cagaatcaga agcatttgac tatctagatg caccaatccg
     6181 ccgttgtgca ggagaagatg tgccgatgcc ttatgcacaa aacctagaaa atgcaatgat
     6241 tccaacagtt gaaagtatca aagatgcaat ccgaaaaact tataacaaag aatagagtta
     6301 cataagataa gttatgtaaa taaaataaag ctgacgagaa actttgtatc ttttaggtgc
     6361 agagctcttc gtcattaata tcttatattc gagcacgggc taaaagctcg gaaaaaagat
     6421 aaatctcctt ggcttcatcg aagccatcgt cgatttccta tttttcagtc gcttttgacg
     6481 ccctttgtat cataaataga attggagagg tcatggctga tgacaagcta agagcgactc
     6541 ctgcagctag aaagttagca gatgatttag ggataaacct ctacgacgtt tctggctcag
     6601 gtgcaaacgg tcgtgtccac aaagaagacg tggaaactta taaagacaca aacgtggttc
     6661 gcatttcgcc acttgcaaaa cgaattgccc tcgaacataa cattgcttgg caggaaatcc
     6721 aaggaaccgg tcatcgtggt aaaatcatga agaaggatgt tttggccctg cttcctgaaa
     6781 atatcgaaaa cgatagcatc aaatctcctg ctcagattga aaaagtggaa gaagtccctg
     6841 ataacgtaac accatatggt aaaatcgagc gtattccaat gacaccaatg cgtaaggtta
     6901 ttgcccaacg catggttgaa tcttacttga ctgcgccaac cttcaccctc aactatgaag
     6961 ttgatatgac tgaaatgttg gctcttcgta agaaggttct tgagccaatc atggaagcaa
     7021 ctgggaagaa gactactgta acagaccttc tttcgcttgc agttgttaag actcttatga
     7081 aacacccata catcaacgct tcattgacag aagatggcaa gactattatc actcacaact
     7141 atgtcaatct tgccatggca gttgggatgg ataatggatt gatgacacct gttgtttaca
     7201 atgctgagaa gatgagtctt tcagaactgg ttgtagcctt taaggatgtt attggccgta
     7261 ccttggatgg taaattggct acaagtgagt tacaaaattc aacattcaca atcagtaatt
     7321 tgggaatgtt tggtgttcag tcctttggtc cgattattaa ccaacccaac tcagctatcc
     7381 ttggtgtcag ttcgacaatc gagaagccag ttgtcgtcaa tggtgaaatt gtgattcgcc
     7441 caatcatgag tttaggatta accattgacc accgtgtcgt agatggtatg gctggtgcta
     7501 agtttatgaa ggacttgaaa gagttgattg aaacgccaat ctcaatgtta atttaagagc
     7561 aagagtattc atttttaaga tatagataaa ggaaggaaga catggcctta gaagtaatta
     7621 tgccaaaagc cggcgtggat atgacagaag gacaaatcgt ccaatggaat aaaaaagtcg
     7681 gagaatttgt aaaagaagga gaaatccttt tggaaatcat gactgataaa gtcagcatgg
     7741 aattggaagc cgaagaagat gggtacttga ttgccattct caaaggagat ggtgaaactg
     7801 tccctgtaac ggaagttatc ggttaccttg gtgaagaaag ggaaaacatc ccaacagctg
     7861 gagcagcatc gccagaagct agccctgtac ctgtagcaag tacctcaaac gatgatggta
     7921 agagcgatga tgcttttgat atcgttgtga ttggtggagg tcctgctggt tatgttgcag
     7981 ccattaaagc tgcccaattc ggcggtaagg ttgcccttgt tgagaaatct gaacttggtg
     8041 gaacctgctt aaaccgtgga tgtattccaa ccaagaccta ccttcataac gctgaaatta
     8101 ttgaaaatat cggtcatgct gcaaatcgtg gtatcgtgat tgaaaatcct aacttcactg
     8161 ttgatatgga aaaactttta gaaactaaat ctaaagttgt taatactctt gttggtggtg
     8221 ttgcaggact tcttcgtagc tacggagtta ctgttcataa aggaattggt acaatcacta
     8281 aagacaagaa cgtcttggta aatggttctg aattgcttga aaccaagaaa atcattcttg
     8341 ctggtggttc aaaagtcagc aagatcaacg tccctggtat ggaatctcca cttgtcatga
     8401 ctagtgatga cattcttgaa atgaacgaag tgccagaaag ccttgttatc atcggtggtg
     8461 gagttgtcgg tatcgaactc ggtcaggcct tcatgacatt tggttcaaaa gtgactgtta
     8521 tcgaaatgat ggaccgtata gttccagcta tggatgtgga agtttctaag aaccttcgct
     8581 tgattcttga gcgtaaagga atgaccatct tgactggtac taaactgcaa gaaatcattg
     8641 aggaaaatgg tcaacttcgt atcaaggttg aaggaaaaga cgatatcatc gcaagcaaag
     8701 ctcttctttc aattggtcgt atgccagacc ttgaaggtat tggagaggtt gagtttgaat
     8761 tggatcgtgg ttgtatcaag gtcaacgaat acatggaaac ttcagttcca ggcatttatg
     8821 caccaggtga catcaacggt actaagatgt tggctcacgc agctttccgc atgggtgaag
     8881 tttccgctga aaatgccctt aaaggaaatc atgcagttgc caaattgaat ttgactcctg
     8941 cagccatcta cactctccct gaagtagcag cagtaggttt gacagaagaa caagcccgtg
     9001 agaaatacga tgttgccatc ggtaagttta actttgctgc taacggtcgt gctattgcat
     9061 ctgacgcagc tcaaggtttc gtaaaagtta tcgctgataa gaaatacgga gaaatccttg
     9121 gtgtacacat tattggtcct gcagccgcag aattaataaa cgaagcatca agcatcatcg
     9181 aaatggaaat cactgttgag gaaatgctga agaccatcca cgggcaccca acttactctg
     9241 aagtgatgta cgaagcgttt gcagatgttc taggaatggc catccattca cctaagaaaa
     9301 aataaaacga agatagatta gactggaagg ttatttccag tttctatcat ataaagtaaa
     9361 gggatatcat gaaatacatt atcaatcatt caaatgacac tgcttttaat attgccttgg
     9421 aagaatacgc ctttaaacac cttttggatg aggatcaaat cttcctactt tggattaaca
     9481 agccatctat cattgttggt catcaccaga acactatcga agaaatcaac cgtgattatg
     9541 ttcgcgaaaa tggtattgag gtagttcgcc gtattagtgg tggtggagct gtttaccacg
     9601 atttaaataa cctcaactac acgatcatct ccaaagaaga tgaaaataag gcatttgact
     9661 tcaagagctt ctcaactccg gttatcaata ccctggctca acttggggtt aaagctgagt
     9721 tcacaggtcg taatgacctt gagattgatg gtaagaagtt ctgtggcaat gcccaagcct
     9781 atatcaacgg ccgtatcatg caccacggtt gcttgctctt tgacgttgat ttgtcagtcc
     9841 tcgcaaatgc ccttaaggtt tcaaaagata aatttgaatc aaaaggtgtg aaatccgtcc
     9901 gtgcccgtgt aaccaatatt atcaatgaat taccaaaaaa aatcacagtc gaaaaattcc
     9961 gtgatttact cttggaatac atgaaaaaag agtacccaga gatgactgaa tacgtctttt
    10021 cagaagaaga attggccgaa atcaatcgca tcaaggatac taagtttgga acttgggact
    10081 ggaactatgg taaatcacct gaatttaacg tccgtcgtgg aatcaaattc actagtggta
    10141 aggttgaagt ctttgctaac gttactgaat caaaaatcca agacatcaag atttatggtg
    10201 acttctttgg tattgaagac gttgctgcag tagaagatgt ccttcgtggg gtgaaatacg
    10261 aacgcgaaga tgttcttaag gcgctaaaaa ccattgatat cacacgctac ttcgctggta
    10321 ttagccgtga agaaatcgct gaagcggtag ttggataaat caaaaagaag ttggccaact
    10381 aaccaacttc tttttttgat atttatttac ataatttaaa atacgtaaaa tcataaacta
    10441 tccagagcat tcttttgttc atcactaaca atatgggtat agaggtcagt gacttgtgtg
    10501 ctagcatgtc ctagttggtg actgactaaa acttgtgatt tagtcgcatc atagagccta
    10561 gttgctagtg tatggcgcag tttatggggt gttacacgca ctttaaaatc ctctgagtat
    10621 ttagcaacca ttttctcaac gctagaagca tcgatacgat taggaacacc tctgtagaga
    10681 gttaaaaaaa gggctgtatc tgttttttcc gttttatagc gttgattccg aatggccaga
    10741 taattctcta aataaggttt agcaaaagca gcgacattga ctgagtcacg tttgcaacct
    10801 tttcgagtaa catcaataac catcattttt agattgagat ctcttagatc tagattaaca
    10861 gcttcagata agcgaacacc agatgccaag agaagggcaa taatggctaa atctcgttct
    10921 ttatttttgt tgaatgatga gagagctcga tttgaaagct gttgtgggtg ctcttgatcg
    10981 atataagtta gaaaaccttc tgtttcatca cctagaaaga gtttttgctt gatattttca
    11041 gctctggcag caagggtttc tttctttttc ttggtggaaa cttttttcat tacattacga
    11101 tagaaataag gttccccctg atcgttttca acctcctcgg ttagatactt gtaaagacta
    11161 gaaagtgctg ataaggttcg attgatagtt gtctgtgaaa caccttgttt tgttgtatta
    11221 gcattcagca agggacgttc acgtagataa aggataaagg attccatgtc tttcttagac
    11281 atattttcca aaactgataa aggaatatcg gatattttat cagcgtttga aataccagac
    11341 tccaaaaccc agctgaaaaa tcggtcatat tccttaaggt attcgtacag ggttgtaaaa
    11401 ctgtagggca cagccagctt agattggtag tattccagaa cataccaggg catgagttgt
    11461 tttagtttgt cgattcgttc cagtaaaatc tcacgtttca tgtattttct ccataagtaa
    11521 gtatactagt agtatagcat agacttgtat atttttcaag aaaattatag tttttcggaa
    11581 agatgttata ggagtttttc aaagtgataa aaagacttag acctacaaac aaaacagaat
    11641 aaggaaaata ataaaaatct cacccactaa acttctgtta atactacata actgaagagc
    11701 tatataagga gctatgagat acaaaacaca ttgtataata gattgaaact agaatagtac
    11761 atctggactt ctaaaacatt gctagaaatt gatttgattg tcctgattgt tttatcctat
    11821 ttctatttca tttctctata taattgctat tgttttcttt ttcatgattt taatcttact
    11881 gtacagatt
//
LOCUS       DAVKHZ010000064        11607 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000064 DAVKHZ010000000
VERSION     DAVKHZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.064"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            124..882
                     /locus_tag="VWW99_001636"
     CDS             124..882
                     /locus_tag="VWW99_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9539450.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            893..1687
                     /locus_tag="VWW99_001637"
     CDS             893..1687
                     /locus_tag="VWW99_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9539451.1"
                     /translation="MKKKFFLSALLISLFSLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSD
                     YGIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1700..2377
                     /locus_tag="VWW99_001638"
     CDS             1700..2377
                     /locus_tag="VWW99_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9539452.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFCIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2387..3046
                     /locus_tag="VWW99_001639"
     CDS             2387..3046
                     /locus_tag="VWW99_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9539453.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVPVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3342..4532
                     /locus_tag="VWW99_001640"
     CDS             3342..4532
                     /locus_tag="VWW99_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9539454.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCSPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4853..5734)
                     /locus_tag="VWW99_001641"
     CDS             complement(4853..5734)
                     /locus_tag="VWW99_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9539455.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5875..7203)
                     /gene="vncS"
                     /locus_tag="VWW99_001642"
     CDS             complement(5875..7203)
                     /gene="vncS"
                     /locus_tag="VWW99_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9539456.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(7200..7856)
                     /gene="vncR"
                     /locus_tag="VWW99_001643"
     CDS             complement(7200..7856)
                     /gene="vncR"
                     /locus_tag="VWW99_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9539457.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7905..7988)
                     /gene="pep27"
                     /locus_tag="VWW99_001644"
     CDS             complement(7905..7988)
                     /gene="pep27"
                     /locus_tag="VWW99_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9539458.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(8168..9547)
                     /gene="vex3"
                     /locus_tag="VWW99_001645"
     CDS             complement(8168..9547)
                     /gene="vex3"
                     /locus_tag="VWW99_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9539459.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9599..10246)
                     /gene="vex2"
                     /locus_tag="VWW99_001646"
     CDS             complement(9599..10246)
                     /gene="vex2"
                     /locus_tag="VWW99_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9539460.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10259..11536)
                     /locus_tag="VWW99_001647"
     CDS             complement(10259..11536)
                     /locus_tag="VWW99_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539461.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3258 a         2605 c         2201 g         3543 t
ORIGIN      
        1 actgtcccga tcgatttttc ctgttcttat ttcactatag tgaatttttt cagatatgat
       61 ttgacaagaa cattctttta ttgtagaacc gtgttataaa atataacaca atagagatct
      121 ctcatggctt tagtagaatt taaaaacgtc gaaaaatatt acggagacta ccacgcattc
      181 cgcaacatca atctccgttt tgaaaaagga caagttgttg tcctgcttgg accttctggc
      241 tctgggaagt ccactcttat ccgtacgatc aatggtttag agactgttga caaaggaagt
      301 ctcctagtca atgggcacca agttgctggt gccagccaga aagatttggt acctcttcgc
      361 aaggaagtcg gcatggtttt tcaacatttt aacctttatc cacacaaagc tgtgttagaa
      421 aacgtaacgc ttgcacccat tgaagttcta ggaattgata aaaaagaagc tgaaaaaacc
      481 gcccaaaaat atctggaatt tgtaaatatg tgggacaaga aagattccta tcccgccatg
      541 ctatctggtg gacaaaaaca gcggatcgcc atcgctcgtg gtcttgctat gcatccggaa
      601 ctcctcctct ttgatgaacc aacatctgct cttgatcctg agactatcgg agatgttcta
      661 gcagttatgc agaaactggc gcatgatggg atgaacatga tcatcgttac ccacgaaatg
      721 ggctttgctc gagaggttgc ggaccgcatt atctttatgg ccgacggaga agttttagta
      781 gatacgacag atgtcgataa cttttttgac aatccaagcg aacctcgtgc ccaacaattc
      841 ctcagcaaaa ttatcaacca cgaaagtgac aaagtcaaat aaggaggcgc ctatgaaaaa
      901 aaaattcttt ttatcagcat tattgattag ccttttcagc cttgctgctg ccaaaccagt
      961 ccaggctgat acaagtatcg cagacattca aaaaagaggc gaactggttg tcggtgtcaa
     1021 acaagacgtt cccaattttg gttacaaaga tcccaagacc ggtacttatt ctggtatcga
     1081 aaccgacttg gccaagatgg tagctgatga actcaaggtc aagattcgct atgtgccggt
     1141 tacagcacaa acccgcggcc cccttctaga caatgaacag gtcgatatgg atatcgcgac
     1201 ctttaccatc acggacgaac gcaaaaaact ctacaacttt accagtccct actacacaga
     1261 cgcttctgga tttttggtca ataaatctgc caaaatcaaa aagattgagg acctaaacgg
     1321 caaaaccatc ggagtcgccc aaggttctat cacccaacgc ctgattactg aactgggtaa
     1381 aaagaaaggt ctgaagttta aattcgtcga acttggttcc tacccagaat tgattacttc
     1441 cctgcacgct catcgtatcg ataccttttc cgttgaccgc tctattctat ctggctacac
     1501 tagtaaacgg acagcactac tagatgatag tttcaagcca tctgactacg gtattgttac
     1561 caagaaatca aatacagagc tcaacgacta tcttgataac ttggttacta aatggagcaa
     1621 ggatggtagt ttgcagaaac tttatgaccg ttacaagctc aaaccatcta gccatactgc
     1681 agattaagga ggacacccca tgacagattt atcatcttgg acagcctatt ttcaggattt
     1741 tggacaattt ttcaatggtt tcctcttcac ccttgcccta gcggttggtt cctttatcct
     1801 cgccatggtc ttaggaatct tctttggtgc catgtcaacc agtaaacgtc caattttacg
     1861 cattttagct cgcatctttg ttgaatttta ccaaaatact cctctcttgg tgcagtttgt
     1921 tattgttttt tatggcctac ctcttatcag tgaccatatc atcatgattc caatttattg
     1981 gacagccgtt ctctgcgtgg gactctatca cggcgcttat atcgctgagg ttattcgttc
     2041 agggattcag tctattccta gtggtcagat ggaggccgcc ttgtcgcaag gttttaccta
     2101 tatcagtgcc atgcgcttga ttaccttgcc tcaggccttc tgcatcattc tacctccatt
     2161 gaccaaccaa attgttaacc tcatcaagaa cacctctact gtagctatca tctctggagt
     2221 agacttgatg tttgtgacta agtcttggtc ggctctcaac ggaaactata tcccagcctt
     2281 tttaggtgct gctcttctct actttgccct atgcttccct gttgcccagt ttggtcgcaa
     2341 gatggagcaa gccaacaaaa aagcctattc actttaggag gttactatgg aatctatttt
     2401 agaagtttta accccagata acctagtctt tatctttaaa ggatttggct tgaccctcta
     2461 tatttctctg attgccatca tcctctctac tatcatcggt acggtgctag ctgtcatgag
     2521 aaatggcaaa aatcctatct tacgcattat ttccagtatt tatatcgagt ttgtgcgcaa
     2581 cgttcccaac cttctctgga tttttactat ctttttggtg ttcaaaatga agtccacacc
     2641 agcaggtatt acagccttta ctctctttac atcagcagcc ttggctgaga ttattcgagg
     2701 cggtctcaat gccgtagaca agggacaata cgaagcagga atgtcacaag gcttcacctc
     2761 agcccaaatc ctctactaca tcattctccc acaagccatc cgcaaaatgc taccagccat
     2821 catttctcag tttgttcccg tgattaagga taccagtctc ctctactctg ttatcgccct
     2881 acaagaactc tttggagcca gccaaattct catgggccgt tatttcgaac cagagcaggt
     2941 cttcagtctt tacatcctga ttgccctcat ctacttcagc tttaacctag caatttctag
     3001 cctgtctcat atgctagcca aacgttggca acaagctgca gaataatact cttcgaaaat
     3061 ctcttcaaac cacgtctgct tcaccttgcc gtatatatgt tactgacttc gtcagttcta
     3121 tccacaacct caaaacggtg ttttgagcag cctgcggcta gcttcctagt ttgctctttg
     3181 attttcattg agtataaaca gccactctcc agttcaattg gagagttttt ttgatgagag
     3241 tagatattta tagacctcaa tttgacacca tacattttct atgctaaaat gtaacaaatt
     3301 tatttacaac aaatcgaaat ctaaatgaaa tggaattttc tatgaaatca atcaaaggac
     3361 tactctttat catagctagt tttatcttga ctcttttgac ttggatgaac acttctcccc
     3421 aattcatgat tccaggacta gctttaacaa gcctatctct gacttttatc ctagccactc
     3481 gtctcccact actagaaagc tggtttcacg gtttggagaa ggtctacacc gtccacaaat
     3541 tcacagcctt tctctccatc atcctactaa tctttcataa ctttagtatg ggcggtttgt
     3601 ggggctctcg cttagctgct cagtttggca atcttgccat ctatatcttt gccagcatca
     3661 tccttgtcgc ctatttaggc aaatacatcc aatacgaagc ttggcgatgg attcaccgcc
     3721 tggtttacct agcctatatt ttaggactct ttcacatcta catgataatg ggcaatcgtc
     3781 tccttacatt taatcttcta agttttcttg ttggtagcta tgccctttta ggcttactag
     3841 ctggttttta tatcattttt ctatatcaaa agatttcctt cccctatcta gggaaaatta
     3901 cccatctcaa acgcttaaat cacgatacta gagaaattca aatccatctt agcagacctt
     3961 tcaactatca atcaggacaa tttgcctttc taaagatttt ccaagaaggc tttgaaagtg
     4021 ctccgcatcc cttttctatc tcaggaggtc atggtcaaac tctttacttt actgttaaaa
     4081 attcaggcga ccataccaag aatatctatg ataatcttca agccggcagc aaagtaaccc
     4141 tagacagagc ttacggacac atgatcatag aagaaggacg agaaaatcag gtttggattg
     4201 ctggaggtat tgggatcacc cccttcatct cttacatccg tgaacatcct attttagata
     4261 aacgggttca cttctactat agcttccgtg gagaagaaaa tgcagtctac cttgatttac
     4321 tccgtgacta tgctcagaaa aatcctaatt ttgaactcca tctaatcgac agtacgaaag
     4381 acggctatct taattttgaa caaaaagaag tgcccgaaca tgcaaccgtc tatatgtgta
     4441 gtcctatttc tatgatgaag gcacttgcca aacagattaa gaaacaaaat ccaaaaacag
     4501 agcttattta cgaaggattc aagttcaaat aagtaatcat actctcctgg gaaatcccag
     4561 gagagttttt tatagccaaa tgaaataaga caggaacgat ggaattttgg cattcaaatc
     4621 aattgataag actattttat agtggagtga aataggatac gaacaaattg attaggaaaa
     4681 tcaaatgaat ttatagaaat cttttagcag ttatgttatc ctattctagt ttcaaaacgc
     4741 tatagaagca gcattgtgct agtctagatt cagtttacta tactaaaacg agtagcttga
     4801 aatcaaaaaa cccaccctca caggcaggtt ttatctgtat tattcagcta gattatgctt
     4861 taccttctga accgaatacg tcgatacgtt cttcaaccga tgcttggata gcttttacac
     4921 cgtcagccaa gaatttacgt gggtcgaaga gttttttctt gtcgtattct gcttcgtttg
     4981 cttcgtagtc acgagcaaat ttacgagttg cgttagcgaa tgcgatttgg cattctgtgt
     5041 taacgttaac tttggcaaca ccaagtttga tagctgcttg gatttgctca tcaggaatac
     5101 ctgatccacc gtgcaatacg attgggaatc ctggaagagc ttctgtcaat ttttgcaagt
     5161 ggtcaaggtc aagaccttcc cagtttactg ggtaaggacc gtggatgtta ccgataccag
     5221 ctgccaagaa gtcgatacca gtttcaacca ttgctttagc gtcttcgatt ggagccaatt
     5281 cacctttacc gatgattccg tcttcttcac caccgatagt accaacttca gcttctactg
     5341 agataccttt agcgtgtgct ttttcaacaa cttctttagc caatttaagg ttttcttcaa
     5401 ctggaaggtg tgaaccgtca aacatgattg aagtataacc aacttcgata cactcaagtg
     5461 catcttcgta gtgaccgtgg tcaaggtgga tagctactgg tacagtgata cccattgatt
     5521 caacaaggtt agcgatcaag ttgcgagcaa ctttgtaacc acccatgtat ttagcagcac
     5581 ccattgaagt ttggatcaaa actggagctt ttttagcttc tgctgcgcgc aagatagctt
     5641 gagtccactc aaggttgttt gtgttaaatc caccaactgc ataaccgttg tcacgggctg
     5701 cttggacaaa tttttctgct gaaacgattg ccattttatc aggcctcctg tatattttta
     5761 tgggtcatcc catttacatt gttcatttta tcactttttg ccaaaaaaat ctagtttttc
     5821 ccgcagtttc gattgatttt cttctaactc catctatgta aaccctttct ctccctagtc
     5881 ttgggcgact tttggaaaag ctataaagaa ggttaaacga ttctcctcca tctcgaaacg
     5941 ataagctaat ttttcatgtt ctaatagact cttaaccaca aagagcccca taccagaccc
     6001 cttgaccttg cgactggcat tgtcagaaaa agactgggct agtttttctt gttcctctga
     6061 gctacagcta ttttcgataa aaagttctcc ttctctttct ccaattcgaa ctaagccacc
     6121 tggaacagag tgcttaatgg cattgctgat gagattagaa agaatcaact tcataactga
     6181 tgggtttaga taagcctgct gatgggtcaa actattgtct atctggagct ctctttcctt
     6241 ggctagcaag gcataatctt tgaccagatt ttgcgtcatc tggaggaggt caattgtttc
     6301 cctatcatct cgcaattcct gcacagaaga gagggaaagt atctgcagaa catggtgatt
     6361 gagttcatcc acaatcccca aggcaactcc cagatactgg tctctatcct tataacgacc
     6421 gatattctct ctcatatttt cgattaggat tttcaaacta gccagcggtg ttttcaattc
     6481 atgagaagct cctcgtagga attcgacctt catcttctcc agctggagaa tggcttcatt
     6541 cttttcatgc aagtccgcaa taacagtcaa gagatgctgg tagaggctat tgatttgttc
     6601 cttgagatta cctatctcat ccttagaatc cacgcgcaat cgcacttggg aatccaggtc
     6661 catcatccga cgggtcaccc gcttgatttc caaaatcggt gcaacaatag tccgggcgta
     6721 gatgtaggcc accaagaggg aaatcagaaa ggaggccagc aaggtatagg gaagaaactg
     6781 gagactgatt tgctccgctt ccttttgtaa atccatggaa gctagaaact ggagaatcat
     6841 agtaccaccg tcttgcgttt tcacctcgcg ctcctcaata aagagagagg ttgtctggcg
     6901 gtctgtgtcc agaggaagac tgtccttgac ttctaacttg tcctcggtca tctcaccttt
     6961 gacggtcccc ttgatatcac tagtctggga atacaagtct aacacttgct cgatactctg
     7021 cctatctttc ccttctaggg actgggcaat ggctgttgcc ttttgaccaa tggtttcctg
     7081 acgatgactc agataagtcg aaggaaaaag aaaataaata gctaaatgaa ggcagataac
     7141 cagaacacta aatatcgaga aggtatagat aaatatcttt gcaaataaac ctgttcgttt
     7201 cattttcgct ccaatttata accaacattg cgcacagtga ggatacaatc caagtctagc
     7261 tttttccgca attccttgat ataaacatca ataacacggt caaagggaac ctcatctgtc
     7321 gctttccaga cggcatcgat aatctgagac cgagtcaagg cccggccttc atttttcact
     7381 agatagtcca gaatttccaa ctctttggca ttgataggca cttcttgacc tgcgaggctt
     7441 gcactgtagc tttcaaagtc caccttggta tccttgtaag aaaagattcg tcctgtatcg
     7501 tagtagcgct tgaaaatcgc gtccaccctc acttttaaga gggagagaga gaaaggtttt
     7561 tccagatagc catctgccaa agaggcaaag gcactcatct tgtattcctc atcctgaaaa
     7621 gctgtcaaca tcaagacagg aacctgactg gttttacgaa tctcagctag gacttctaag
     7681 ccgttaagct tgggcatctg gatatccagt aaaaccaggg ccacctcata gctagaaaat
     7741 tgctccagag cttcctgacc gtccgctgcc tcaatagttt catagccaca atccgtcaaa
     7801 taatcactga ccccctcacg gatcatctct tcatcttcta caattaaaat tttcatactt
     7861 taactgctct ctatttttta tttttcttag aataaatacc tactctattt tctattatag
     7921 tctcttgctg gccttttgtc tgtaagcaac tgatcactag ataaaacgtt gtgaaattcc
     7981 tttctcataa attccataac tttagtatat tatatttaag cactaaagta caaagaaagc
     8041 aactgaaagc aatgattttc accactgctt tcagatttat tttgaattgt taaatagcta
     8101 ttcctatcca ctattcttga atagaaacac aagatgcaat ctttattcca gactcatttt
     8161 ttcaaattta ttcaccatcc agcaagagct cttttggttg ttttctaagg agattgcttg
     8221 aagcaagcgc cataacgaga accactagaa ccaaggcaag gacaaaaatg atgataaagt
     8281 ctgatgtctg aatggaaatg tctaggctcg acaaggtctt gctaaagcca tctacttctg
     8341 caccgccacc aaggttagag gcttgagccg ccttactagc ctgtttggca acacctgaag
     8401 tcacattggc aaggacagtg tttccaattg cacgggcagt gtaattagct aggaagtaag
     8461 cagaaactag agcagggata gcaatcaaga tagattcggt gatgaattga cccaagatac
     8521 ttgcctgctt gaggccgata gagaggagaa ttcccacttc cttgcgacgg gcgttgatcc
     8581 aaaggctgag caagagggca aggaggagaa ctgagaagct caagctaccc cagaagagga
     8641 ggttggccat cttgtacata ccagagatag attgctcaag agctgggtag ttagaggagc
     8701 tcttgacgag tgtgtagctc ttccagttga taccactgat gccattcaac tctttcataa
     8761 catcatccaa gttcttgtct gctgttacaa agaaggttgc gtccccataa atggctgtgt
     8821 cttctgtgta tccataaagt tttgcagcag tgtgaatgtc tgtaatagct gtgttttcgt
     8881 aaagttcttg tgagtaggtt actgctgact tattatgacc atcaaagagt cccttgattg
     8941 tcacttcaac tgtttccttg gctccttttt cattatctgc atcgtagata ttagagtcca
     9001 gtttaacctt gtcccctact ttccagccgt gtttggctgc caagtccttg tgcaagagga
     9061 ttttatcctt gtcgtcgttg gttaagtgct ctccttcgac tagtttataa gaaccagaga
     9121 caaacttatc ttctttagag gagtcattga cacctgtaat catcaagcta cttccaaaac
     9181 gcttggcacg atcagcagtg agattcttct tggtttctgg cgtttcaatc aggtcatatc
     9241 cagtcaaatc tccgatagcg ttgatacgtt tgacataaga ctcgatggcc ttgttttcgg
     9301 tgattttttt gatgtcttca cccttgatat tcccagcacc acgaggcgtt ccttggttga
     9361 cgcgacgatt gatttgcatg gagaagctat tggtgatatt tttaaaggtc tcctgagaag
     9421 ccttggcagt agctcccttg attgacaagc cgaccaaact caagctcgcc atgaggagaa
     9481 taatcaggaa gatgacaatc gatttgaaaa acttccttgt aacataggca aatgcgttgt
     9541 gtaacataga ttccctttct agattttgtt ttaatcattc tatcaaaata agctcaaatt
     9601 atttactagt attgcgcgtt tcagtcagtt tcttatcctt taattcaagt gtaatatctg
     9661 acgcttgtgc cacttcttta ctgtgagtta cgacaatcac acatttacct gttttctggg
     9721 caagtgattt gagtagttcg acaatatctc cagcagtttt aggatccaga tttcctgttg
     9781 gctcatcagc tagaataact ggagcttctg agaccaaact gcgagcaatg gcaacacgtt
     9841 gctgttgacc acctgataac tggagaacat tccgcttgat ctggctttca tccaaaccaa
     9901 gctcaagaag tgtattcttg cttgcctttt tgttgaccaa tcggatattt tccagcggag
     9961 aaagataatc tatcaagtta taattttgaa agaccaggga aatatggtgc atgcgatggt
    10021 aagaatagcc cttcttacga atatcctctc cttgaaaaag gatagaacct tcaacaggac
    10081 tatctagacc agcaagtagg gacaagagtg tggattttcc tgctcctgac tccccaataa
    10141 tactgtaaaa ttttccgggt tcaaaattat aatcgatctg atataggact gcttcagcag
    10201 tattcttata acggtaggta acatcttgta attgtaataa agtcatgatt tctccttctt
    10261 aactaataga tgataaaatt tctttcggtg attttctaaa taagaatagg aaacaaaggg
    10321 ctacagataa gcaactaagc agaactagaa aaacatagga ttctgcaaaa gataagatac
    10381 tagttgataa actgcttgct ttggctagtg tatcttgtaa gcttgcctga tctccacttg
    10441 ctagtagagt ttggagtagg taagttgtga ttccgtttcc tgcaacaaat gctggaagca
    10501 aagctccaag agataccaaa actacctcta aacagaattg taggaagatc gagctcttgc
    10561 cttttccaag tgcaagtaaa atccccactt catagacccg ttctctcaac cagagagaca
    10621 aaaccagaat taaggctcca gctcctgcta tcaacatccc ataaaggaag atggtcagga
    10681 aggtttggaa agttgcaact gagtctttga tttgttcaaa agccttgttt tccttttcga
    10741 cttggtagcc ttgattttcc aaggccaagt tttctacctg cttcatgagt ccgtccattt
    10801 ccttaggatt ttctacatag aagcgtgctg cactgacttg agcttcacta ttgcccaaaa
    10861 gggtttggct actttcatag tctgtaaaga cttgattttc actgaagtca gaagacaagc
    10921 ctgtgaattt ctcttgtttt ttaccagaaa agatgccgat aatctcaaac tctactgttt
    10981 gtccttttcc agattcagac tgaccagcat ccaagccaat cttgtcatga agtgaaagac
    11041 cgttcttctt agccaattct tcgtggataa ggattttctt ggaatcccct ttttgaaggt
    11101 gtcgcccttc ttttagattg aaagccgaac tggtaaaggt tacatccttg gatgaatcct
    11161 caagagccgt taagctaacc aagttattgt ctgcagctga taaatcatca cgctccacgc
    11221 tctgctcgcc agtcactgct tccttgtctt ttagttttgc gaccgtctca agttcaggag
    11281 agacattttc cagcccctta atcttgctta cagatgctag gtctgacaac ttgaatgtct
    11341 gaccattctc tatcttctta atagaaaaag atgtattgag tgatttataa agattgcttt
    11401 ctactgtttt gttggacttc atcagagtca aacaggctga aattccggcc aataagacca
    11461 ataaaatcag aaataaaata aaacttctca gtcgctttct gctgacataa gcccaagatc
    11521 tttggattgg attcatttgt cacctccata tttgtaagac tattataaaa cccaaatatg
    11581 aaatgtttat gaaatacgaa aaaaaaa
//
LOCUS       DAVKHZ010000065        11500 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000065 DAVKHZ010000000
VERSION     DAVKHZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.065"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(56..286)
                     /locus_tag="VWW99_001648"
     CDS             complement(56..286)
                     /locus_tag="VWW99_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9539462.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VWW99_001649"
     CDS             complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VWW99_001649"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9539463.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1113..2036)
                     /locus_tag="VWW99_001650"
     CDS             complement(1113..2036)
                     /locus_tag="VWW99_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9539464.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2033..2722)
                     /locus_tag="VWW99_001651"
     CDS             complement(2033..2722)
                     /locus_tag="VWW99_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9539465.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(2787..3491)
                     /locus_tag="VWW99_001652"
     CDS             complement(2787..3491)
                     /locus_tag="VWW99_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9539466.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(3547..4569)
                     /locus_tag="VWW99_001653"
     CDS             complement(3547..4569)
                     /locus_tag="VWW99_001653"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9539467.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(4701..7172)
                     /gene="parC"
                     /locus_tag="VWW99_001654"
     CDS             complement(4701..7172)
                     /gene="parC"
                     /locus_tag="VWW99_001654"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9539468.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDVAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(7592..9535)
                     /gene="parE"
                     /locus_tag="VWW99_001655"
     CDS             complement(7592..9535)
                     /gene="parE"
                     /locus_tag="VWW99_001655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9539469.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLADKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            9671..10312
                     /gene="plsY"
                     /locus_tag="VWW99_001656"
     CDS             9671..10312
                     /gene="plsY"
                     /locus_tag="VWW99_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9539470.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(10355..11500)
                     /locus_tag="VWW99_001657"
                     /pseudo
     CDS             complement(10355..11500)
                     /locus_tag="VWW99_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3261 a         2565 c         2038 g         3636 t
ORIGIN      
        1 ctctcctaca atcaacatgg actattatat catgaaaatt tcaaaaaaac aagacttatt
       61 ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt ttataaaaac
      121 tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca atttctccga
      181 taactttttt accgatagtc aatgtataac cttcaaaact gtctttacca acattaactt
      241 tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca tacctctctt
      301 cactttttct attttacaag aaaatcctaa aactagcaag aaaaaactct atatcaggct
      361 tctctgatat agagttttat gtaaacaacc tcaatcgatt tagcatagct aatagttagt
      421 aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc ctactaactg
      481 caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc ctcttacaat
      541 atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca tcatatatgg
      601 aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat aaagagcttg
      661 gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg ccggaagtcc
      721 ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat aggccgatgc
      781 tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag gtatgcgagc
      841 atcatcttga ttaatagcca ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc
      901 aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa cttctgacga
      961 cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa tctcagatgg
     1021 taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag gattaaaacc
     1081 tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta cattaagaat
     1141 atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac gccattctga
     1201 tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat tggggtcaat
     1261 aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg attagctccc
     1321 agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc tgcaaaagca
     1381 tttcccataa tcattgtgaa tagaaccatt ccaagaacat aggccaaaac tcctataaag
     1441 cgactatctg aaggaacaat accgctaatc agatgagaga taacatcacc aacacctgct
     1501 acagtaaaaa tagcccccaa agcccctaat aattgaggaa caataccact tgttgaaact
     1561 tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt ggtaatcaca
     1621 agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct aaattctgga
     1681 atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat aaaaatttta
     1741 tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg caaggttccg
     1801 atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat accaatactc
     1861 atatttggca tataggaacc acctataaac gtaatagaca atagagtcca aaatgcagat
     1921 gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt atggagaaat
     1981 tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc tgtcattttt
     2041 gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa ttgtaaatcc
     2101 ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc cctgcataat
     2161 tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc ccagcaccta
     2221 caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgctgccgca cgcgctttta
     2281 ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca gctgcttctc
     2341 ccataggttg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga attgaaaaga
     2401 aaccagctaa ctctcgaata aagaaataaa ctgtatagaa gtttccaact gtcagacctt
     2461 taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct gacagcccca
     2521 caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat tcttttccca
     2581 aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc aaaccagcta
     2641 agactactgt tgcaattgta tcaaatttta aaataaaacc cacaacaatg attgctattc
     2701 ctattaatct aatccactcc atatcaaact cctttatatt caaaatgaca gtattttaaa
     2761 aattttatca agatcaatac cattccttat ttaatgtatt tttctagttc tttttggtat
     2821 ttgctattgg attccaattt ttctttttgc cattttttaa aaacctcgtt atattctttt
     2881 gttgtaacaa tatctttttg caatttcatt cctttaaaga tatatggttc ccccttaata
     2941 ccaacttgtg agtatggttt tgagaatggt actacgttac ttacaactgg agaaccacca
     3001 gatgaagctg ttggcatcaa taatgaacta tctgtcgacc aagcttgagc tttggcatat
     3061 ttttcatatc tcttctctag gtcagtggtc tcagaaacag catcttctaa caatttctta
     3121 tatttatcca aaccaggttt agctacaaca tccttatctt ttcctttcgt aataccaagg
     3181 tgtttcatgg cagaaccaga ttttggatcc ataatattca agtaagacgc tggatcttga
     3241 tagcttggag cccatcctgt actattcaaa tcatagtctt tttgagaagg agcaacattg
     3301 ccgtatttat cattttccat caaaccatca ataacatttc caataacgtc tgtcctcgat
     3361 gttcgagtcg ctatactgta gcccaatgat gctggatcta ctgcatagac ataagaaaat
     3421 gttgtcggtg catctgcttc tttatcagtt tttccacaag ccactaaaat agctgacgtg
     3481 ctcaggacca ctcctgctgt taagagccac tttttctatt tcataaaaaa tctcctttgg
     3541 tttattttaa tctactttta caatccaacc ttctggcgct tcaatatcgc caaactgaat
     3601 acccgtcaat tcattatata atttacgcgt cacaggacct acttctgttt cactatagaa
     3661 tacatggaaa tcatcaccat gttgaatacc tccaattgga gaaataaccg ctgctgtacc
     3721 acaggcacct gcctctacaa aacggtcaag attatcaatt ggaacatcac cctcaatagg
     3781 agttaatccc aagcgatgtt ctgccaaata aagcaaggaa tacttggtaa tagatggcaa
     3841 gatagatgga ctcaatggtg ttacaaattc attatcagct gtaattccaa agaagttagc
     3901 tgatccgact tcttcaatct ttgtatgagt tgatgggtcc agatagataa catctgagaa
     3961 atgacgtgac ttggccattt ttcctggtaa gagacttgca gcatagtttc caccaacctt
     4021 agccgcacct gtaccatttg gtgctgcacg gtcgtactca tcctgaatca agaagttggt
     4081 tgggaccaaa ccacctttaa agtaatttcc aactggcata gcaaagatgg tgaaaatgta
     4141 ctcttctgcc ggttttaccc cgataatatc tccgacacca atcaaaagag ggcgaagata
     4201 taaggttcca cctgttccgt atggtggtac gtattcttca ttcgcacgga caactgcttt
     4261 acaagcttct acaaacatgt ctgtcggaac ttgtggcatc aagagacggt cacatgtacg
     4321 ttgcagacgt ttagcatttt catcaggacg gaacagttga acactgccat ccttagtacg
     4381 ataagctttc aaaccttcaa atgcttgttg tccatagtga agacttggag aagactctga
     4441 aatatgcaaa gttgcatcct ctgtaagctc tccttgatcc cattgtccat ttttgaaatg
     4501 agcaagatag cgataaggta atttcatata ggaaaaaccg aggttttccc aatcaatcgt
     4561 tactgtcata ttccactcct tattctaaaa acctatttct tatattctac actatttttc
     4621 taaaatagca agtatatttt gtaattttca gaaaatttct ccaataaaaa ccagctctta
     4681 gaactgattc ttcatttcac ttatttatct tcagtaacta cttcctgaag ataagcgtca
     4741 aaaacttctt catctgaaat cgtgtcagaa atgaagcttc cattgctagt gcgttctgac
     4801 aagttcaagt cttgcaatcg gctttcatag attgttcctt tattggattg gacaagcaga
     4861 gtttggtcgt tcacatccac ttccgtactg aagaaatcgc caacaaatcc ttgctctgca
     4921 actgctcctg ccaagaagac acgatgcggt ttgtttttca actcacgcaa gacttgtaat
     4981 cctcgtttgg cacggctggt tgctagaatt tcctcaatgg aaacacgttt caagcttcca
     5041 cgctgggtca agaggtagaa ggacgaagta ttacagataa agccagattg gaggacatca
     5101 tcttctttca aattcatagc cttgacacct gctgccttag caccgacaac cggaacctct
     5161 tcgatattga aacgcagggc ataaccattt tgactaacca agacaacatc atctagttta
     5221 atcggagcca ctgctacaat ctgatctgta tcgtctttga gcttagcata cttgacagac
     5281 ttagatctat aggtccgcca tggagtgaat tcttttcgct ctacccgttt gatttgacca
     5341 aggcgagtca ctgcaaagta ggttgtcgca tcgtcaaact gatccagtac ttccacataa
     5401 aggatttctt cattcgtttc aaagtttgtg atggtttggc tcagatgctc tccgatgtcc
     5461 ttccaacgaa tatctgccaa ctcatggatt ggtctgtaga tgacatttcc aagacttgtg
     5521 aacatcaaga ggtgctgggt tgtcttggca gattgaacaa aaatcaaacg gtcatcatca
     5581 cgcttgccaa tttcttccaa ggtggaagcc gcaaaggaac gtggactggt acgcttgatg
     5641 taacctgcct tggtcacgct gacgtaggta tcttcctcag cgataagact agctgtatca
     5701 atctcaattg ctttcgcagt gtcttctaaa gaactcaaac gaggagttgc aaatttcttc
     5761 ttgacctcac gaagttcttt cttcatgaga ttgtacatag tcctttcatc accgataata
     5821 gccgccagca tagcaatctt ctcacgaagc tctgcttctt cttcctgcaa gacaaccaca
     5881 tcggtattgg tcaaacggta cagttgcaaa gttacgatag cctcagcttg ttcttccgta
     5941 aaatcatagc taactttgag gttttccttg gcgtccgcct tattctcaga agcacggata
     6001 agagcaatga cttcatccaa aatcgaaatc acacgaatca aaccttcgac gatatggaga
     6061 cgtttctcag ccttttcttt gtcaaagcgt gaacgcgcca aaatcacttc tcgacggtga
     6121 gcgatgtagc tagacaggat tggaacaatc ccaacctgac gaggtgtgaa attgtcaatc
     6181 gccaccatat taaagttgta gttgatttgt aggtcggtgt acttaaataa gtagttgaga
     6241 acaagctcag tattagcgtc tttcttaagt tcaatagcga tacgaagacc atcacggtca
     6301 gactcatcac gaacctcagc aatcccagct accttgttat taacacgaac atcatcgatt
     6361 ttcttgacta gattggcctt attgatttca taaggaatct cagtaataac gatttgttcc
     6421 ttaccacctt ttagcttttc aatttcagtc ttggaacgaa caaccacgcg ccctttccca
     6481 gtctcataag ctttcttgat ttcatcacga ccctgaataa tagcccctgt agggaagtct
     6541 ggtccaggta agaattccat gagtttatca atctttgcag ttgggtggtc aatcatgtaa
     6601 actgcaacat ctatgacctc agctaaatta tggggaggaa tgtctgtggc ataaccagcc
     6661 gaaatcccag tcgaaccatt gaccaagagg tttggaaagg ctgctggcaa gaccgttggt
     6721 tctttctccg tatcgtcaaa gttccatgca aaaggaactg tctttttctc gatatcctga
     6781 agaaggtagc ctgcaatttc agacaaacgt gcctcagtat aacgcatagc cgcaggagga
     6841 tctccgtcca tagaaccgtt attaccgtgc atttcaacta gaatctcacg atttttccag
     6901 ttctgtgaca tacgaaccat ggcatcatag atagaagaat ccccgtgtgg gtggaaattc
     6961 cccatgatgt tcccgactga cttggccgac ttacggtagc tcttgtcaaa agtattgcta
     7021 tccttattca tagaataaag aatacggcgc tgaaccggct tcaacccatc acgaatatct
     7081 ggcaaagccc ggtcttgaat aatgtacttg gagtagcgac caaagcgctc tcccatgatg
     7141 tcctccaggg acatgttttg aatgttagac ataagataca aagcccataa aataccaagt
     7201 gaaaatagaa aattcttgaa gtaagcaaac tcacaagaga atttatcttt ttcacacagt
     7261 atctagggcg tgttcaactc ctttcaaaga atgtagagta ggtttttatg cagtaaaaga
     7321 tattttacgg gaattcgtcc cgtgttcagt tacgataagt aaccaaacta tcctgtttgt
     7381 atttttcaat atgaaaatat ggttttccaa aattagtctt agtttgtgtc ttagccgctc
     7441 ccttaaacgc ctctttgaga taagcactca tagcagattc ttcattaata atcctgcaat
     7501 tttttcaaac caagattttc aaactgcttt ttcacatagt cattcacatc cgactctaat
     7561 ttccagttta ctaacatatt attttctttc attaaaacac tgtcgcttct tctagcgtaa
     7621 acttgacatt atcttcaatc catttacggc gtggttctac cttatctccc atgagaacat
     7681 tgacgcggcg ttcggcgcgc gctaaatctt caattgtgac acggatgagg gtacgtgttt
     7741 ctgggttcat ggttgtttcc cagagctggt ccgcattcat ctcaccaagt cctttgtatc
     7801 gttggagggt agcgccttta ccgaactgtt tacggagttc ttctagttct ccgtccgtcc
     7861 aagcgtaggc cacttcttct ttcttgcctt tacctttgga catcttgtaa agaggtggga
     7921 gggcaatata gacatgacct gcctcgacta gcggacgcat gtaacggtag aaaaatgtca
     7981 agagcaaggt ctggatatgg gcaccgtcgg tatccgcatc ggtcatgata atgatcttat
     8041 catagttggc atcttcaata gagaagtctg ctccaacacc cgcaccaatg gtataaatca
     8101 tggtattgat ctcttcattt ttgaggatat ccgccatctt ggccttggct gtattgacaa
     8161 ccttaccacg aagaggtaga atagcctgga acttgcggtc acgaccttgt ttggcagaac
     8221 caccggcaga gtccccctca actagataga gttcattctt agcaggattc ttagattggg
     8281 ctggggtcaa tttcccagac aacaagccct tatctttctt gtttttcttc ccatttcggc
     8341 tctcatcacg cgccttacgt gctgcttcac gagcatcacg ggccttgata gccttgcgga
     8401 tgaggttaga agctaattcc ccattttcca taaggaaaaa ggtcaactta tcagccacta
     8461 ttccatccac aactgggcga gctagggggc ttcctagttt atccttggtc tgtccttcaa
     8521 actgcaagtg ttcttcagga actaagatag aaagaacggc cgctagtccc tcacgatagt
     8581 ctgaaccttc aaggttttta tctttttcct tgagaagacc tgttttacgt gcatagtcat
     8641 tcatgacctt ggtaatggca gacttgagtc ctgtctcgtg cgttccaccg tccttggtgc
     8701 gaacgttatt gacaaaggat agaatgttat ctgagaatcc gtcattgtac tggagggcta
     8761 cttccacttg aaaaccattg tcttcccctt caaagtaaag aactggcgtc aagatttcct
     8821 tatcttcgtt gagataagaa acaaaatctt gtactccatt ctcatagtgg aactcaatcg
     8881 cttcatctgt tcgcttgtcc gctaaagaca aggtcacatt tttcaagaga aaggctgatt
     8941 cattaaggcg ctctgaaatg gtattgtact tgaaatctgt cgtagaaaag atagtcgcgt
     9001 caggcataaa agtaactttg gtgcctgttt tagacttgag tgctgtaccg attttcttca
     9061 aagtcgtgac aggttttcca ccattttcaa aacgttgctt gtaaactgcg ccatcacggg
     9121 taatttcaac ttctaaccag ctagaaaggg cgttaacaac ggaagaaccc actccgtgaa
     9181 gtccacctga tgtcttatag ccaccttgac cgaatttccc tccggcatga agaatggtaa
     9241 agataacctc aacagttgga attcccatag cgtgcatacc tgtcggcatc ccacgtccat
     9301 ggtcttgaac cgttagacta ccgtctttat tgatagttac atcaatacga tcaccaaacc
     9361 cagacaaggc ttcatcgact gcattatcaa cgatttccca aactaggtga tgaagaccag
     9421 cgccatcggt cgatccaata tacatccctg gacgttttcg gaccgcatcc aacccttcta
     9481 gcacctgaat agcatcatca ttataattgt taatattgat ttcctttttt gacacaagga
     9541 acctcctatt cgttcatctt tactattcta caggttttcc aaggattttg caaaattttt
     9601 ctttctccga tgtgacaatt tcagcagaga ttctctgctt ttctttccca attcatgata
     9661 taataggagt atgattacaa tagttttatt aatcctagcc tatctgctgg gttcgattcc
     9721 atctggtctc tggattggac aagtattctt tcaaatcaat ctacgcgagc atggttctgg
     9781 taacactgga acgaccaaca ccttccgcat tttaggtaag aaagctggta tggcaacctt
     9841 tgtgattgac tttttcaaag gaaccctagc aacgctgctt ccgattattt ttcatctaca
     9901 aggcgtttct cctctcatct ttggactttt ggctgttatc ggccatacct tccctatctt
     9961 tgcaggattt aaaggtggta aggctgtcgc aaccagtgct ggagtgattt tcggatttgc
    10021 gcctatcttc tgtctctacc ttgcgattat cttctttgga gctctctatc ttggcagtat
    10081 gatttcactg tctagtgtca cagcatcgat cgcggctgtt atcggggttc tgctctttcc
    10141 actttttggt tttatcctga gtaactatga ctctctcttc atcgctatta tcttagcact
    10201 tgctagtttg attatcattc gtcataagga caatatagct cgtatcaaaa ataaaactga
    10261 aaatttggtc ccttggggat tgaacctaac ccatcaagat cctaaaaaat aaaacgccag
    10321 ttctgtactg cccccaaaca gttagacaaa taatttatcc aaaggattta gttctgtatt
    10381 gcacagggct aagtcctttt agttttacct taattcgttt gttgttgtag taatcaatat
    10441 agtctataat ggcttgttcc aattggttaa gtgatttaaa tgttttctca tagccataaa
    10501 acatttcgga ttttaaaatg ccaaagaaag attccatcat accgttgtct tggctgtttc
    10561 ccttgcgtga catagatgct tgaattccct tactctctag gaaccgatga taagaatcgt
    10621 gttgatattg ccagccttgg tcactatgga gaatcgtatt ctcgtagtgc ttctctgtaa
    10681 aggcctgttc taacatactc ttcacttgtt ctaagttggg cgaagttgaa agattatagg
    10741 cgataatttc gctattaaag ccatctaaaa ctggtgataa gtaaagcttt tgagtactta
    10801 ctggaatggc aaattcagtc acatctgtgt agcactgttc cattgtttta gagccttcaa
    10861 attggccttg aatgagattc tctgctttct tgccaacgtc tcctttatga gaagaatatt
    10921 ttcgtttctg tcgcgtttta gcttgtaaat tgagtacttc atcaagtgtt gaactctttt
    10981 atgatttacc agataaccac gatttcttag ttctaaataa atccgacgat aagcataatt
    11041 tcccttgtgt tcgataaaga tggattgaat ttcagcttta agctcttggt ccttatctgt
    11101 tttgtctagc tgtttcaagt gatagtagta ggtcgaacga gctagtttaa tggcttttag
    11161 aagaagatct aacgaaaact cagtcattaa ttcttgaaca atttctgtct ttcttctttc
    11221 tctttttcct ccttcaatcg gagttctctt aactttttta ggatggcatt ctccgctctc
    11281 aggtactcat tttctgcttg aagacgttct aattctgtcc tctcttcagg tctcgttttt
    11341 ggattacgtc ccattttagg tactctccct cttggtttct caacaatagt atacccgttt
    11401 ttcctgtatt gtgctagcca gttaagaagt atcgtacgac ttgggagacc gtattcaaga
    11461 gaaactctat ctttagtcca gccttcatgt cagactttat
//
LOCUS       DAVKHZ010000066        10813 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000066 DAVKHZ010000000
VERSION     DAVKHZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.066"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            75..425
                     /locus_tag="VWW99_001658"
     CDS             75..425
                     /locus_tag="VWW99_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9539471.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREDDTH"
     gene            640..756
                     /locus_tag="VWW99_001659"
     CDS             640..756
                     /locus_tag="VWW99_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9539472.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            749..982
                     /locus_tag="VWW99_001660"
     CDS             749..982
                     /locus_tag="VWW99_001660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539473.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLASEVASQQLGMSVSEFEALL"
     gene            1109..1390
                     /locus_tag="VWW99_001661"
                     /pseudo
     CDS             1109..1390
                     /locus_tag="VWW99_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1524..2492)
                     /locus_tag="VWW99_001662"
     CDS             complement(1524..2492)
                     /locus_tag="VWW99_001662"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9539474.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALSEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2637..3179)
                     /locus_tag="VWW99_001663"
     CDS             complement(2637..3179)
                     /locus_tag="VWW99_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9539475.1"
                     /translation="MDQLLGVLFPDSTFLNAFESAIVAPLVEEPLKLLPLVFVLALIP
                     VRKLKSLFLLGIASGLGFQMIEDIGYIRTDLPEGFDFTISRILERIISGIASHWTFSG
                     LAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAIPVVTAI
                     ALYGFYHAYCFVEKHNELMT"
     gene            complement(3183..3482)
                     /locus_tag="VWW99_001664"
     CDS             complement(3183..3482)
                     /locus_tag="VWW99_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539476.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFHIGKKWELPKKVLILSLLGAICSFTSLLLFGIYNHRRKSSKV"
     gene            complement(3507..4004)
                     /locus_tag="VWW99_001665"
     CDS             complement(3507..4004)
                     /locus_tag="VWW99_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9539477.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            complement(4077..5438)
                     /gene="radA"
                     /locus_tag="VWW99_001666"
     CDS             complement(4077..5438)
                     /gene="radA"
                     /locus_tag="VWW99_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9539478.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(5452..5967)
                     /locus_tag="VWW99_001667"
     CDS             complement(5452..5967)
                     /locus_tag="VWW99_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9539479.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGEMLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5969..6412)
                     /locus_tag="VWW99_001668"
     CDS             complement(5969..6412)
                     /locus_tag="VWW99_001668"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9539480.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAAGGVRTGGFGSTGH"
     gene            complement(6472..6565)
                     /gene="ffs"
                     /locus_tag="VWW99_001669"
     ncRNA           complement(6472..6565)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VWW99_001669"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6598..7065)
                     /locus_tag="VWW99_001670"
     CDS             complement(6598..7065)
                     /locus_tag="VWW99_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9539481.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(7266..8552)
                     /locus_tag="VWW99_001671"
     CDS             complement(7266..8552)
                     /locus_tag="VWW99_001671"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9539482.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(8783..9019)
                     /locus_tag="VWW99_001672"
     CDS             complement(8783..9019)
                     /locus_tag="VWW99_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9539483.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRKDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            9285..10081
                     /locus_tag="VWW99_001673"
                     /pseudo
     CDS             9285..10081
                     /locus_tag="VWW99_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10116..10813)
                     /locus_tag="VWW99_001674"
                     /pseudo
     CDS             complement(10116..10813)
                     /locus_tag="VWW99_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3457 a         2502 c         1804 g         3050 t
ORIGIN      
        1 ataaattaat ttgactttcc tgatagagtt attcacatct tatttcaact cactatagaa
       61 ggaggaatag gaggattctc agacatccgg gcatcagccc aactaatgat ttgattgcta
      121 agaaaatatt cagcaatcca gaaatcactt gtcaatttat tcgcgatatg ctggacttgc
      181 cagcaaaaaa tgtgaccatt ttggagggaa gcgatattca cgtattactc tccatgcctt
      241 actcggtgca ggatttttat accagtatag acgtcttggc ggagttggat aacggtactc
      301 aagtaattat tgagattcaa gtccatcatc agaatttttt catcaatcac ttgtgggctt
      361 acctgtgcag tcaggttaat caaaatcttg aaaaaattcg tcagcgagaa gatgatactc
      421 actagatcta caaacacatc gctcctgttt acgtcattgc tatcgtggat agtaattatt
      481 tctcagatga cctggctttt catagcttta gtatgcgcga agacacaaca ggtgaggtat
      541 tggcgattac caacaatgga caggaaaacc atctggttaa gatggcattc ttggaattaa
      601 aaaatacaga gaaaccagca aagacaaggt tcgcaagcca tggttggagt ttttcggcaa
      661 caagcccttt acccagcaac cgcaacgagc cattacccaa gcaaatcaac tgctggacta
      721 caagagctgg tccgaggagg acaggaaaat gtttagtcaa ctacatatgc gagaagaaca
      781 agtcttgtta gcacaggact atgccttgga aactgctagg gctgaaggcc ttgaacaagg
      841 actagagcgt gggaaagttg aaggaagggc agaaaggaaa ctttttgcct tcctagacat
      901 agtacgccaa ggtcttctgg cttctgaggt tgccagccag caattaggta tgtcagtatc
      961 tgaatttgag gcactgttgt aaaatggctc cataatatcc ataggggatt tacccactat
     1021 ggatattatg gagcctattt tgtgtaaaaa aaaagtccca tatgacctat aatgaaaagc
     1081 gacaaaacaa ctcattagaa agaatcatat ggaacaatta cattttatca caaaattact
     1141 agacattaaa gaccctaatg tccagatttt aaacatcatc aataaggata cacacaagga
     1201 aatcatcgcc aaactggact acgacgcccc atcttgccct gagtgcggaa accaattgaa
     1261 gaaatatgac tttcaaaaac cttctaaaat tccttatctt gaaacgactg gtatacctac
     1321 tagaattctc cttagaaagc gtcgattcaa gtgctatcac tgttcaaaaa tgatggtcgc
     1381 tgaaacttct gatgacgtac agtcatattt cttctctttt tattatatca cagttttaaa
     1441 tctagcttta ctagattcac cgctactatc tatttattcg gaaaaaagac gaaaaaacct
     1501 gagaatcatc tcaggcttgg tcattaaatt tttttctcaa tatcgaaaag tggagaaagt
     1561 ggtcgttttt catgaatacg tacgatagca tcccctagga gatgagcgat tgaaatctgc
     1621 tcaatcttat caatcaaacg ctcttctggc agatagatgg tatccaaaac aaccaatttc
     1681 ttaatagctg atttttggat attgtccgta gcaggaccag aaagaactgg gtgcgtacag
     1741 cttgcataga cttcaacagc accagcttcc gaaagagcat ctgccgcatg acaaatcgtt
     1801 ccagcggtat caatcatatc atcaatcaag atacaagtct tgccttcaac cttaccgatg
     1861 atgttcataa cttcactagt attcatctta tcaacgctac gacgtttatc aataatagcg
     1921 atagatgttt tcaaaaattc tgccaactta cgagcacgag tcacccctcc atggtccggg
     1981 ctgacaacca catagtcaga accaaccata ccacgacgct caaaataatc tgcaatcaga
     2041 ggagcaccca tcaaatgatc cacaggaata tcaaagaatc cttgaatttg cgcagcatgc
     2101 aagtcgatgg tcaataaacg atccactcca gctacttcaa gcatatttgc gacaagtttt
     2161 gaagtgattg gctcacgcgc tctcgccttt ctatcctgac gtgcataccc atagtaaggc
     2221 atgacaacat tgacagattc tgcactcgca cgcttcaaag catctaccat aatcaaaatt
     2281 tcaagcagat tgtcatttac aggcgaacta gttgattgta ggataaagac gtgtttccca
     2341 cggattgatt cttcaatgtt gacctgaatc tctccatctg aaaattggcg aacacttgat
     2401 ttccccaact ctatcccaat ctcctgcgct acacgttctg ccaattcttt attagaagaa
     2461 agggcaaaca gctttaaatc agaaaaagac atgatttcct ccggtatata tgtataactt
     2521 gtgcttttca caagattttc catctaccat tgtagcgctt tttgcactat ttttcaatca
     2581 aaaataaaag aagggcacca tatttgtacc cttgcatcat tcttttgaaa aatattctag
     2641 gtcatcaact cattgtgttt ctcaacaaag caataagcat gataaaaacc atagagagca
     2701 atagccgtaa ccactggaat cgctaaaggc aactctgttt ccaactccac aaaaggagag
     2761 ttaaacaaga agtgagttcc caaggctaaa cctagaaaaa taaggccctg tttcttgcca
     2821 accttctgtc ctttataggc tctgtaaagc aagtaaacac ctactacagc tagacctgaa
     2881 aaagtccagt gagaggcaat tcctgagatg atacgctcta aaattcgcga aatagtaaag
     2941 tcaaagccct ctggcaaatc cgtacgaata taaccaatat cctcaatcat ttggaatccc
     3001 aaaccggaag caattccaag taaaaacaaa gattttaatt ttcgcacagg aatcaaagcc
     3061 aaaacaaaaa caagtggcaa taatttcaag ggttcttcta ccaaaggagc cacaatagca
     3121 ctttcaaagg catttaaaaa tgtactatct gggaaaagaa cccccagtaa ctggtcgatt
     3181 ttttatacct tagatgactt tcgtctgtga ttatagattc caaatagtaa gagagaagta
     3241 aaggaacaga ttgctcccaa taaagacaga atcaaaacct tctttggcaa ttcccatttt
     3301 ttcccaatat ggaagagaaa ataaagagcc ggaatcatgt aaaagagagc tagaaagata
     3361 gaaactccca ttagtccata ttccgcacct gacctcgaac cgtccgtata gtagatggtt
     3421 tcatactgta aaccaataca tagcaataaa ataaaaataa ataaaatact gcttttcttc
     3481 atacactttc tttctaaatg aagtatttat aattctacga ctgccatact tcctgtatca
     3541 acattgtaaa tggcaccaga gataatgaca tcgtctggta ttaggggaga ctcgataagc
     3601 agttgcatat cctcgcgtac actctcttct atatcttgga agggcaagaa gtcctggtct
     3661 gacacatcta cacctaattc ctcttttaaa tactcctgaa aaggttcatt ttcaaaggtc
     3721 tgagcaccac agtctgtatg gtgcaatacc acaatctctc ttgtccccat ttgttgctgg
     3781 gaaataacta gcgaacgaat catgtcttca gtcactcgac cacctgcatt ccgcaagata
     3841 tgagcatccc caagtgccaa gcccagagct tgcgcaacgt gcagacgaga gtccatacag
     3901 gtcacaatag ctactcttgt tttgggttta agtggcagat ttaactgccc atgtagggca
     3961 acataagcct gattagcttg cataaactgt tcaaaatacg acacgattcc ctccttgaaa
     4021 atttgatagt caaatatttc tcctatctta tcatttttaa gagaatttgt cacggattat
     4081 gcaaagacct ttttcaaaac ttcctgaatc gttgtcacgc caatgacctg aatttcctta
     4141 ggcagagtga ttcctgtcaa ggaattctta ggtacataaa tcttagtaaa gcccagttta
     4201 gcagcttcgt tgatgcgttg ctcaatacga ttcacgcgcc gaatctctcc tgtcaagccc
     4261 agttctccga caaaacattc ctgaggatta gttggcttgt ctttgtagct cgaagcaata
     4321 gcaactgcaa cagccaagtc aatcgcaggt tcatccaatt taacaccacc agcagatttg
     4381 agataggcat cctgattttg caagagaagc cctgcccgtt tttccaaaac agccataatc
     4441 aagctagcac ggttaaaatc aagtcctgtc gtagtacgct tggcatttcc aaacatggtc
     4501 ggtgttacca aagcctgaac ctccgccaaa atcggacgcg tcccttccat ggttacaacg
     4561 atggaggaac cagtcgcccc atccaaacgc tcttctagga aaacttgact cggattgagt
     4621 acctcaacca agccgcccga ctgcatctca aaaatcccaa tctcattagt ggaaccaaaa
     4681 cgatttttga ccgctctcaa aatacgaaag gtgtggtgac gctccccttc aaagtaaagc
     4741 accgtatcca ccatatgctc caacatacga ggcccagcca aggttccttc tttggtcaca
     4801 tgacctacga taaagatggc aatgttattg gtcttggcca actgcatgag ttcagcggtc
     4861 acttcacgca cctgagaaac agacccctgc acccctgaaa tctcaggaga catgatggtc
     4921 tggatggaat caataatgag aaagtctggc tggatacgct ccacttctgc acgaacactc
     4981 tgcatattgg tctctgcata gagataaaac tcactatcaa tatcacctaa gcgctctgca
     5041 cgtagtttaa tctgctgggc agactcctcc ccactgacat agagaactgt ccccacttgg
     5101 gacaactggg ttgagacttg taggagaaga gttgatttcc caatcccagg atccccaccg
     5161 atgaggacga gacttcctgg taccactccg cctccaagca cacggttgaa ttcctccatc
     5221 tccgtcttag ttcgattgac attgatggaa gtcacctcag ctagtttcat gggcttggtt
     5281 ttctcacctg tcaaggacac acgcgcattc ttgacctcgg caacctcaac ctcttccaca
     5341 aaagaagacc aagacccaca gttggggcaa cgtcccagat atttaggaga attataccca
     5401 caattttgac atacaaatgt cgcttttttc tttgcgatga caaacctctt tctatatctc
     5461 taactcacac tcaatcactt ggcaaaaatc aatcttctca tttggcacaa actggcgcat
     5521 gagcattcga tgagcaacaa ctaccacagt ctgatgttct cgatacttag acatacattc
     5581 tagaaaccga gacttcattt ccgtagctgt ctcatattga ataggactat taggaagcat
     5641 ctcccccttg ttttctaaaa acagtcttct agctgtttca aagttttcta ttcctgtttt
     5701 atagacctgc cattcatgta ataaaggctc tactcttaaa ggaagacccg tagcacagac
     5761 cacatacgaa gccgtttcta aagctcttgt gactgcagaa gatacgatta tttcagctga
     5821 cgagagtaaa ggatttttgc tcaatttctg gacttgctgc cgtcccatct cagacaaggg
     5881 tgccaaatct atcccaaatc ctatataaga acgctcctct aactcacggt aatctggctc
     5941 cccatgacgt acaaagataa tcttcattct agtgccctgt cgatccaaat ccaccagttc
     6001 gaacgccacc agctgcatct ccatctgcaa ttaagaaagt agcaaaaaca gcctggacaa
     6061 tacgctcccc aacttcaaga acaacctctt ggtctgtgat attcttcatc tgcgcaaaaa
     6121 tatgcccttc atttccagga tttccataat aatccccatc aatgacccca actgagttaa
     6181 ttaaaaccaa gcccttctta cgaggatttg aagaacgatc atagaggtag agaacctcag
     6241 tcggctgcat ataagcctta acccctgtcg gaaccaagac aatctctcct ggcgcaacaa
     6301 ctgtacgcac agcaaccttt aagtcgtaac cagccgcatg cgctgtctca cgcttgggca
     6361 ataaattttc atctgtaaaa ctcgaaacca attcaaaacc acgaattttc ataattttct
     6421 cttttctatt atcatttatt ctagattatt ctatcttatt tattcggaaa aagcacgaaa
     6481 aaagagcaca caattcaaat cgcttagggc tgctggattc ctcccctgac ccgcttcacg
     6541 cagaactgtt gctccactat ttattatatc acattcctat tcattttaaa agcaaaatta
     6601 ttttttccgt ctatttctaa aaaaatcctg catgatagct gcgcattcat cttccaaaat
     6661 tcctatttca acctccacac gatggttaag acgctcatct gtcaagatat cgtacaaact
     6721 cccagcagcg ccaaatttct ggtttttagc cccatagacc acatttggaa tgcgggcaag
     6781 cccaatcgcc ccactacaca tgacacaagg ttcaatggtc acaaaaagtg tgcaatccag
     6841 caagcgccag ctctcctcac tcaagttcgc atcctctata gccataattt ccgcatgcat
     6901 aaccgctcgc tgtaattcct cacgcgcatt atgcccacga ccaatgattt ccccatcttt
     6961 gacaatcaca caaccaattg gaatttcatc gtgttcaaga gcaatctcag cctctctcaa
     7021 agcctccctc ataaagactt ctttttcttc aagcgtataa tacatctctt ttctcttttc
     7081 ctacttatcg attttattat tatatcatga atcccaacac aaaaaaagcc accgaatgcg
     7141 gtgactctat agggagatta ttatgaaaaa gaaaagttta ggatatttgt tacaacaagt
     7201 taggaggtct tcttgtaacc gtctatagta tacccgacct atcttaaaca aatcttaaaa
     7261 atctcttagg accaaacact ttctaaaata tttgtttgtt cacgaccagg acctactgag
     7321 aaagtagaaa tacgaacgcc aaccaattca ctcacacgac gaacatagtt acgcgcattc
     7381 tcaggaagat cttccaaatt gcgaactccg gtaatatctt ctgaccaacc tggcaactct
     7441 tcatagatag gcttgcaacg tttcaattgc tcaagactag ctggatagta gtcaatacgt
     7501 tgaccgtcaa gatcataggc cacacagatt ttcacagtat ccaaaccgct caaaacatca
     7561 atagagttca aagaaaggtt agtaatacca gaaacacgac ggctatgacg catcacaact
     7621 gagtcaaacc aacctacacg acgtggacga ccagttgttg taccatactc atgacccact
     7681 tcacggatac gttctcccac ttcatcaaac aactcagttg ggaaaggacc atctcctaca
     7741 cgactcgtat aagctttaca tacacctaca accttgtcaa tcttgcttgg accgacacca
     7801 gaaccaattg tcacaccacc agctacaggg tttgatgacg taacaaatgg ataagtacct
     7861 tggtcgatat ctagcataac accttgtgca ccttcaaaaa gcacacgttt gccattatca
     7921 agcgcatcat tcaagataac agatgtatct atcacgtatt tcttgatttg ttgaccatat
     7981 tcgtaatatt cttcaaaaat atcatcgaaa acaatcgctt tactgtcata caatttttca
     8041 aaaagacgat tcttttcagc aaggttacgt tctaaacgct cacggaaaat atctttatct
     8101 aaaagatctg caatacgaat tccaacacga gcagccttgt ccatataagc tggaccaatt
     8161 cccttaattg tcgtaccaat cttattgtcg cccttagctt cttcttgcaa gcgatccaac
     8221 tcgatatgat aaggcaaaat aacatgcgca cgatcagaaa tacgcaagtt atcagttgtt
     8281 acaccttcct catgaagata gctcaactct tttacaagag atttaggatt tacaaccata
     8341 ccattcccaa tgacagatat tttttcaggg aagaaaatcc cagatggaat caagtgcaac
     8401 ttaaatttct taccgtcaat cacaatcgtg tgaccagcat tatcaccacc ttggtaacgt
     8461 gcaatcactt ctgcattcgc tgaaaggaag tctgtaatct tccctttacc ttcatcaccc
     8521 cattgggtac ctacaacaac aactgaagtc ataatcttgt ctgagccctc aggctcttcc
     8581 tttctcacat acatggcagg actctcacct gcaattatat cttacaattt attataataa
     8641 aaaatcgcct ttttatcaag aagaaacaat agaaagattt gctatttcca actattaaaa
     8701 aatgatttag aaaaattact agctatttac tatcatcttt ccataaaaga gtaaattagt
     8761 tcggaaattt actaaaatta cctcaacaag aaataaaccc ccgattcatt accaattttt
     8821 caagatacaa acgataagca acacgataat ggtaaacaat aaaatcctta cgacaaccaa
     8881 tgccatatct cactaaataa taaattaaaa atttaaaatg aacatgttcc cagtcaaaat
     8941 tatcaccaaa tgtaggacca tactcttctt caatactatc atagaaatta gccatctgct
     9001 cataaatttt ttgtaacata atcaaatact ccttttcttt tttataaact tattctaaca
     9061 aaaaatttta cacattcact atcaattcct gaattgttaa aataccagcc tcaacaagat
     9121 aaaaatagga aaagttgaca aaatagaaac aaatttgcta cctaaatttc aaaaaattaa
     9181 agttctaaaa agaccgcctt aaaacttttg ggtaagtcct attagaacta taatattttt
     9241 atataggtat tatggttctg attttctagt ataaaaaaat tcaaactaga attcttctta
     9301 gaaaaggccg ttttaagtgc tatcattgct caaaaatgga gctcgctgag acttctatcg
     9361 tcaagaagaa tcatcaaatt ccttgtatca tcaaccaaaa gattgctcag aagctaattg
     9421 aaaagacttc tatgaccgac attgatcatc agttgtctat ttcaacttca actgtcattc
     9481 gcaagatcaa taattttcac tttgagcatg atttttcgcg tcttcctgag attatgtcct
     9541 aggacgttga aacagtccgg ggagtgactg tttcaatcgg ggatggagat gagctttatt
     9601 gctcaggact ttgataaact caatatcata actgttcttg agagcagaac acaagccatc
     9661 atccgaaatc ccatgaatac aaggctatca agcgctactg gaagctcatt caacaagata
     9721 gtcagaaact gagtgataaa cgattttatc gccctacttt tcgtatgcac ttgactaata
     9781 aagaaattct agataaactt ttgagctatt ccgaagactt gaaacaccac tataatctct
     9841 atcagctctt gcttttccac ctctgtttca aactttgttt aaaaccttcc tcaaagataa
     9901 agagaaaatc gtcaacgccc ttcaattacc ttattccaac gccaaactgg aagccaccaa
     9961 taatctcatc aaacttatca agcacaatgc ctttggtttt aggaactttg aaaacttcaa
    10021 aaaagaaagg acgaaatttg tcctttctag atcttcgctt tcttcaaccc actacagttg
    10081 acaaagaacc gaaaaatgtc tcgcctctaa tatagtcaat tgaaacaaga acaagagaaa
    10141 agagcctcgt aaaaggtatt gcaacttggt aatacctttt tgaggttctt tttgatataa
    10201 gcccatgttt tctcaatagg attgtactca ggtgagtagg gaggaagagg taaaagttta
    10261 tgcccaaact cctcgcataa aagttctaac ttacccattc tatggaatct tgcattatcc
    10321 ataataataa ccgatggtgt ggttaatgtt ggtaagagaa acttctgaaa ccaagcttca
    10381 aaaaagtcgc tcgtcaccat ctcttcgtaa gtcattggag cgattaactc accatttgtt
    10441 agacctgcaa ccaaagaaat cctctgatat cttcttccag atactttacc tcttattaac
    10501 tgacctttta atgagcgacc atattctcga taaaaataag tatcgaatcc tgtttcatca
    10561 atctaaacag gtgctaggtg ctttaaacta ttaaaatttt taagaaataa ggctactttt
    10621 tctgggtctt gttcatagta ggtgtggttc tttttttcga gtgtagccca tagctttgag
    10681 cgcatagtgg atggtagttg gatgacagcc aaattcagaa gctatttcag tcaaataagc
    10741 gtctggattg tcagtaagat agtttttaag tctatctcta tcaacctttc ttggttttgt
    10801 tccttttact tgg
//
LOCUS       DAVKHZ010000067        10762 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000067 DAVKHZ010000000
VERSION     DAVKHZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.067"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            195..692
                     /locus_tag="VWW99_001675"
     CDS             195..692
                     /locus_tag="VWW99_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9539484.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            692..2347
                     /gene="dnaX"
                     /locus_tag="VWW99_001676"
     CDS             692..2347
                     /gene="dnaX"
                     /locus_tag="VWW99_001676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9539485.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2375..2569
                     /locus_tag="VWW99_001677"
     CDS             2375..2569
                     /locus_tag="VWW99_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9539486.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2719..3489
                     /gene="sufC"
                     /locus_tag="VWW99_001678"
     CDS             2719..3489
                     /gene="sufC"
                     /locus_tag="VWW99_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9539487.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEGDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3517..4779
                     /gene="sufD"
                     /locus_tag="VWW99_001679"
     CDS             3517..4779
                     /gene="sufD"
                     /locus_tag="VWW99_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9539488.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4790..6016
                     /locus_tag="VWW99_001680"
     CDS             4790..6016
                     /locus_tag="VWW99_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9539489.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6003..6443
                     /locus_tag="VWW99_001681"
     CDS             6003..6443
                     /locus_tag="VWW99_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9539490.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6497..7909
                     /gene="sufB"
                     /locus_tag="VWW99_001682"
     CDS             6497..7909
                     /gene="sufB"
                     /locus_tag="VWW99_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9539491.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8108..9349)
                     /locus_tag="VWW99_001683"
     CDS             complement(8108..9349)
                     /locus_tag="VWW99_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9539492.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9452..10561
                     /locus_tag="VWW99_001684"
     CDS             9452..10561
                     /locus_tag="VWW99_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9539493.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3178 a         1967 c         2344 g         3273 t
ORIGIN      
        1 atatgattct ttctaatgag ttgttttgtt gcttttcatt ataggtcata tgggactttt
       61 tttctacaca aaataggctc cataatatcc ataggggatt tacccactac aaatattata
      121 gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg ctcctttttt
      181 gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg ttaaatgaag
      241 aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc aacgccagtg
      301 ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg ttcgatggaa
      361 aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt gcactaggca
      421 agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga gatgtgacga
      481 cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg gtgccgatga
      541 tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt gaggattacg
      601 atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa aagacaacat
      661 gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc gaaaatatag
      721 aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc ttaaacaagc
      781 ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg gaacgggaaa
      841 aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag tgggtggcga
      901 accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag aagatgtcat
      961 tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc gtgataaatc
     1021 tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg ttcacatgct
     1081 gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac agaatgtagt
     1141 ctttattttg gccactactg aattgcacaa gattcctgct actattctat cccgtgtgca
     1201 acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc actatatctt
     1261 agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca ggcgggcgga
     1321 aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga cacagggaaa
     1381 tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat cagccttgga
     1441 tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt gcttgaatct
     1501 tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc actatttaag
     1561 agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag tctttgtaga
     1621 aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa cagtgagttt
     1681 agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga ccgtccgttt
     1741 ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa ttgctacgct
     1801 gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg ttcctaaaca
     1861 agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg tcgatcgcaa
     1921 taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac gtcaaaatct
     1981 aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc cggacaaggc
     2041 tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc ttgcttttga
     2101 gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca tgtttggtaa
     2161 tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca tggaggaatg
     2221 gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg aaaaagaagt
     2281 agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga aggtagagga
     2341 agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat ttattattat
     2401 ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga ctggccgcta
     2461 tattatggca gccttttggg caattttact ctttagaaat ttccgagtca gttatgtgat
     2521 gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc cctcagcttc
     2581 cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta tatttataga
     2641 atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt aaaagttttg
     2701 aaaaagaaag gaactatcat gtcagtatta gagattaaag atcttcacgt tgagattgaa
     2761 ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat tgccgctatc
     2821 atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg aaatccaaac
     2881 tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga gttggaagtg
     2941 gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga aatccctgga
     3001 attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaagg tgatgagaag
     3061 atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct caacatgaaa
     3121 gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa aaaacgcaat
     3181 gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga gattgactca
     3241 ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat gcgtggtgaa
     3301 ggttttggtg ctatgatcat cactcactac caacgtcttt tgaactatat cacacctgat
     3361 gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga attggctgcg
     3421 cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga ctacaaggaa
     3481 gaattgtaat ttcctcgtat cttttaggag aagtaaatga ctagagaaaa tattaaactt
     3541 ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa agcttttgac
     3601 aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg ttggaatctg
     3661 ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt cacagcttta
     3721 gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca aactccagtt
     3781 gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga agaaattcca
     3841 gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa gttggcggct
     3901 taccacacag cttactttaa cagtggtgct gtactctata ttccagataa cgtagaaatc
     3961 acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc gtttaacaag
     4021 catattatga ttatcgctgg taaaaattct aagattagtt atctggagcg tttagagtca
     4081 cgcggtgaag gaagtgacaa agcaactgcc aatatcacag tggaagtgat tgcacgttct
     4141 ggtgcgcaag tcaagtttgc tgctattgac cgtctaggtg aaaacgtcac tgcctacatt
     4201 agccgtcgtg gtaaattagg caacgatgca agtattgact gggctatcgg tgtcatgaac
     4261 gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag ccatgctgac
     4321 ctgaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac tcgtgtaact
     4381 aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct tgaaaaagca
     4441 actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc agatgcgcaa
     4501 caagagagcc gtgttcttat gctttcagac caagcgcgtt cagatgctaa cccaattctt
     4561 ttgattgatg aaaatgacgt aactgcagga catgcagcct ctattggtca ggtagatcca
     4621 gaagacatgt attacctcat gagtcgtggc ttggataagg caactgcaga gcgtttggtt
     4681 gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt tcgtgatgaa
     4741 atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta tgttagatgt
     4801 agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg aacctctggt
     4861 ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag ctattaacag
     4921 ctactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag cggaacgagc
     4981 gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg caggctctac
     5041 aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg cacgctttgc
     5101 tgaggaaatt ctcgctgagg gagaccaggt cttgatttca gtaatggaac accattctaa
     5161 tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct atgtctatct
     5221 taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata aggttaaatt
     5281 tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca aggaaatcac
     5341 tcaattagcc caccaagttg gggcaattat ggtagtggat ggtgctcaat ctacacctca
     5401 tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg gtcacaagat
     5461 ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg agcaaatgtc
     5521 tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatttg ctagttggaa
     5581 ggaattgcct tggaaatttg aggctggaac gccaaatatg gcaggagcta ttggacttgc
     5641 gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc atgaacagga
     5701 attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca tttacggttc
     5761 tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc tccatcctca
     5821 cgatcttgcg acggctctgg attatgaagg agtggctgtt cgtgctggtc accattgtgc
     5881 gcaacccttg cttcagtatt tggaagtccc agcaacagct cgtgcaagtt tttatatcta
     5941 caataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa aggagttttt
     6001 caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac cattcgaaaa
     6061 atccacatca ccaagggaag ttagaagatg ctgagcaaac cagtctcaac aatccgactt
     6121 gtggggatgt catcaacctc tctgtcaagt ttgatgcaga ggaccgtttg gaagatattg
     6181 cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg acagatgccg
     6241 ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct gaaatggttc
     6301 aggggcaaaa agatgagcgt caaggccaac ttggagacgc ggcattcttg tcaggtgttg
     6361 ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt aagaaaacaa
     6421 ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta ttagaaatga
     6481 agaaagaaag gatactatgg ctgaagaaag agtagaacca aaaccaattg accttggtga
     6541 atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa aaggactcaa
     6601 cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga tgttggagtt
     6661 ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg gagcagactt
     6721 gtcagagatt gactttgatg acttaatcta ctaccaaaaa ccatctgaca aaccagcccg
     6781 ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg ggattccaga
     6841 agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag tggtttacca
     6901 caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag attccgcact
     6961 caaggaatac ccagacttat ttaaacaata ctttgcgaag ttggtaccgc cgacagataa
     7021 caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct acgtgccaaa
     7081 aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg aaaatatagg
     7141 tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact acgtagaagg
     7201 atgtacagca ccaacatatt caagcaatag cttacacgct gccattgtag aaatttttgc
     7261 tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata acgtctataa
     7321 cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga ttgatggaaa
     7381 cttgggtgcc aaaacgacta tgaaatatcc atctgtttac cttgatggag aaggagcgcg
     7441 tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca cgggtgctaa
     7501 gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca tcgctaaagg
     7561 tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta agaaatctgt
     7621 ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag atactattcc
     7681 atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag tatctaagat
     7741 ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg aggcaactga
     7801 aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg aatacgcagt
     7861 tgagctgaac cgcttgatta gctatgaaat ggagggatca gttggataaa atttgatttt
     7921 atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg tatggttact
     7981 gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg cggctagctt
     8041 cctagtttgt tctttgattt tgaagatgaa cttgtatcaa aaaatcgcgg tttaaaatcg
     8101 cgatttttta taatttctcg ttaacaaagc ggacaaactg attccaccaa acttttaaga
     8161 agaaggcttt ttcaattttc ttgtctgcta ccatttcgaa actagggcgc tctgtggtga
     8221 tgtaaccttg accaatcaag tccttgtctt cataagtcaa atggccaacc actgttccag
     8281 cttcaagtgg tgctgggatt gctttggaat caggtgtgaa ttgaacagat tgggaagatt
     8341 gattcccaac acgttctatt agatagatat cctctggagc cactgcagtt actgtatctt
     8401 cttttccatc ttgtacaggg gttttgctat cttgataggc atcgccttgt tgaacgattt
     8461 tgcgaagtgt aaatgtagaa gaaatataat ccattaggga agatgtagct gtaaatcgag
     8521 cgtaaggatt attgtcttga tgatctgcat ttaaaacaac tgtgatgact ctcatgcctt
     8581 tttcgacagt agtaccaaca aaagactctc cagccttatc tgttgttcct gtttttagcc
     8641 catcaaaacc accacggtaa gcaggcatac cttctaacat gtagttggtt gaagtgattg
     8701 tcatcccagc aaaagtagaa gaaggttttt tggtgatttc taagacttgt gggtattttt
     8761 tgatgaggtt gcgagcaacg atagcgacat cataagcact aagcttattt tcctcatctt
     8821 ttttagaacc tgggtaaatg ttatccccta gagtttcatt gttaagacct gtcgtattga
     8881 caacagtggc atcctgaatt ccccattcca agagttttgc ccgcatcata tcgacgaaat
     8941 ctttttctga gccagcaatt ttctcagcta gggcaatagc ggcgctgttg gcactagata
     9001 ccagagttgc ttcaagcaac tcttcgacag tataattacg ggcctccata ggaatattac
     9061 tggcttcaga atttgtcgtc aattgataag gataatcaga aatatctaca ggagtggaga
     9121 gggtaatact tccgttttcc aaagcttcat agaccagata aacagtaatc aattttgtta
     9181 tggaagcaat ttcgacaggt tgcgttgcat ccttctcata gagaatttta ccagtatttg
     9241 cctcaacagc aatcgcatgt ttagcggcaa tggtaaaatc ttgagcaaca gcagtagaag
     9301 caccccctaa aagagagaca gttaacaaag ttaaaaatat ttttttcata gtagtcttat
     9361 tctatcataa agaaaaaaaa tattcttgct ttaataattc atctgttaag ctttttgaaa
     9421 atatggtaaa ataaagtgag ggaggtaact catgtttcgt agaaataaat tatttttttg
     9481 gaccacagaa attttactct taaccatcat cttttaccta tggagacaga tgggatcttt
     9541 gattaaccct tttgttagcg tgcttaatac aattatgatt ccatttttat tagggggctt
     9601 tctttattat ttgacaaacc ctattgttac tttcttaaat aaagtctgta aactcaatcg
     9661 tttgcttggt attttaatta ccttgtgtac tttggtctgg ggaatggtca taggtgttgt
     9721 ctatctctta cctattttga ttaatcagtt atctagtttg attatatcta gtcaaactat
     9781 ttatagtcga gtacaagact taatcataga cttatctaat tatcctgcgc tccagaattt
     9841 ggatgtagaa gctacaattc agcagttaaa cttatcctat gttgatattc ttcaaaatat
     9901 cctaaatagc gtatcaaata gtgtggggag cgtcttgtca gctcttatca gtactgtttt
     9961 gattttgatt atgactccag tttttttggt ttatttctta ttagatggac ataaattctt
    10021 gcccatgctt gaaagaacga ttctaaagag ggatcgcttg catattgcag gcttattaaa
    10081 gaatttaaat gcgacgattg ctcgctatat tagtggagtt tcgattgacg caatcattat
    10141 aggttgtttg gcttatattg gctatagtat tattggttta aaatatgctt tagtttttgc
    10201 cattttttct ggtgtagcca atttaattcc ttatgtgggg ccaagtattg gtttgattcc
    10261 tatgatcatc gcaaatatat tcactgatcc ccatagactg ctgattgcag tgatttatat
    10321 gcttgttgtt cagcaggtag atggcaatat cttatatcct cgaattgtag gaagtgttat
    10381 gaaggttcat ccaatcacga ttttagtttt acttttgttg tcaagcaata tctatggtgt
    10441 agttggaatg attgtcgcag tgccaaccta ttctatcttg aaagaaattt ctaagttctt
    10501 atcccgtttg tatgaaaatc ataaaataat gaaagaacga gaaagagaat tagctaagta
    10561 aaagtcagga gaaccctgat ttttctttac tggaagtggc ctttagatta gaagactgaa
    10621 aataagttaa agtcttaaac taattttcac agctaagaat agtagaagtt aatctgataa
    10681 aaatcgaaaa aaccagtgga attccgtgtc agggtaagtt ccactggttt tcatagtcta
    10741 ttaaagttcg aatgaaacct at
//
LOCUS       DAVKHZ010000068        10760 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000068 DAVKHZ010000000
VERSION     DAVKHZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.068"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(54..470)
                     /locus_tag="VWW99_001685"
     CDS             complement(54..470)
                     /locus_tag="VWW99_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539494.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(482..1177)
                     /locus_tag="VWW99_001686"
     CDS             complement(482..1177)
                     /locus_tag="VWW99_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539495.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1371..3872)
                     /locus_tag="VWW99_001687"
     CDS             complement(1371..3872)
                     /locus_tag="VWW99_001687"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9539496.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMVVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4327..5415)
                     /locus_tag="VWW99_001688"
     CDS             complement(4327..5415)
                     /locus_tag="VWW99_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9539497.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5433..6101)
                     /locus_tag="VWW99_001689"
     CDS             complement(5433..6101)
                     /locus_tag="VWW99_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9539498.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SSSI"
     gene            complement(6120..8558)
                     /locus_tag="VWW99_001690"
     CDS             complement(6120..8558)
                     /locus_tag="VWW99_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9539499.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(8709..10007)
                     /locus_tag="VWW99_001691"
     CDS             complement(8709..10007)
                     /locus_tag="VWW99_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9539500.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(10024..10332)
                     /locus_tag="VWW99_001692"
     CDS             complement(10024..10332)
                     /locus_tag="VWW99_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9539501.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(10378..10698)
                     /locus_tag="VWW99_001693"
     CDS             complement(10378..10698)
                     /locus_tag="VWW99_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9539502.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         3296 a         2344 c         1765 g         3355 t
ORIGIN      
        1 actatataat actagaccat ccttaaattt tgcttaagaa aaaacaagtt ttgttatttt
       61 ttacgattca tccaagtcat ccctatacaa ttataggtgg ataagatttc aaagcccata
      121 gaacgataga agcccaaggt tctttctgtc tcttctgtca ccagctggac ttgataggca
      181 tctttgtaat cctctaaagc ctctttcatc aaggcactac caattccttg acgctgatag
      241 attggtaaaa cgattaaatc ctgaaccaat actgatgaga atccatctcc aaccaaacga
      301 atcaagccca ccacagcatc accatcaagt gccagataaa ttactaatga atgagataag
      361 gcctgctcca gcatctctgg ttgatgggta taatttgtcc aaccgacagc ctgatagaga
      421 tgtaaaacat cctctagctt gacaatttct tgctttttaa tagtaatcat ctcaacacct
      481 cttaaagttc tctcaagctc ttgtactgct gtccattttt atcaaaattt tcaggacgca
      541 accatgtttc caaacgatct ttgacttgag gccagtcctt atcaatcata gacaaccaat
      601 ccgtatctct cgtacgtccc ttataaacca ctgcctgacg gaaggttcct tcataaataa
      661 agctcaaacg ctccgcagct cgtctggatg gcagatttaa ggcatcgcat ttccactcgt
      721 agcgacgata gtttagctcc tcaaaaacat agcgagctag gagatactga gcttcagttc
      781 ctatccgtgt accccttagc tctggagaaa aagtgactgc tccgacttct attactcggt
      841 tattctggtc aatgcgcatg agagaaaaag atcccaaagc cttaccagtc gccttgtcta
      901 tgattgcata gtaaaaacgg tccttacgag ccaacatctg atgtaaaagg ctaaccagct
      961 cccccatatc tgctactggc tcctgaaaaa ggtaggtcca catctcccga ggagtatccg
     1021 ggccataaac agctagcaaa tcctccgcat gcttttccac tgaaagagcc tctatcctag
     1081 cataacgccc ttctaagaaa tcaatagaag gcagttcacc tggtgtataa ccttccattg
     1141 actccccaat catttgacca tattcattta ctggcatagc tttctccttt tttcactttt
     1201 caaactttat agttgcccat agtataccat actttcataa gaagattcaa aaaatcctcc
     1261 agattctact ctgaaggatt cctaccttat ttcacaacga tattaacgag tttagttggc
     1321 tcaaacagtt tgggaaactg tttgaggttg gaaatcagca aataaattgg ttattttaca
     1381 acaatattaa ccaatttatt tggtacacta atcactttca cgatttcctt accgtcaatt
     1441 tctgctttga ctttttcatc cgctagagca atttcttgca attcttcacg tgataggtct
     1501 ttagcgacca tgagtttggc acggacttta cctttgattt ggacaacgat ttcgatttcg
     1561 tcttcaacca gtttactttc gtcccaagtt ggccaagcta cataagagat tgactcacct
     1621 gtttctgcga ctgtttgcca gagttcttct gccaagtgag gtgcaaatgg tgcaatcaat
     1681 tgaataaagc ctttggcata gtcaacataa agcttatctt ccttgttagc agcattgaca
     1741 aagaccataa gttgggcaat agctgtgttg aatttgagag actcaatttg ctcagtaaca
     1801 gctttgactg tttcgttgta agccttgtca agagcaccat tgttttccgc aaggatttct
     1861 ttacttgtaa tcaaacggta aactcggtca aggaacttac ggcttccttc caaaccttct
     1921 tctgaccaag caatcgaagc atcgagtggt cccataaaca tttcataaac acgaagggta
     1981 tcggcaccgt attgttccac cacatcatct gggttaacaa cgttcttgag cgatttagac
     2041 atcttggctg gcgcttgctc caactcttcc cctgtttcta tatgaaagaa ggaaccatca
     2101 cgtttttcaa ccttgtcggt tgccacaaga gcaccacggt ggtcacggta gcttgttccc
     2161 aaaatcatcc cttggttaaa gagtttttgg aatggttcct tagtcggaac aacaccgagg
     2221 tcatagagga atttatgcca gaaacgagca taaagcaagt gaagtacagc atgttccgca
     2281 ccacccacgt agatatctac tggcaaccat tgtttgagga ggtcctcatc agccaatttc
     2341 tcagtattgt gcgggtcaat atagcggagg tagtaccagc ttgaaccagc ccattgtggc
     2401 atggtgttag tttcacgacg acctttgaca ccatcttcac gagtcacttc aagccaatct
     2461 gtcaagttag ctagtggact ttcaccagta cctgaaggac ggatatcctt ggttacaggc
     2521 aagacaagcg gcaattcagt ttcaggaaca gctgttgaag ttccatcttc ccaatgaatg
     2581 attggaattg gctcacccca gtaacgttga cggctaaaga gccagtcgcg gaggcggtag
     2641 gtaaccttct cctgaccaca gcctttttct tccaagcaag ccacaatctt ggcaatagcg
     2701 tcttctttgt tcaatccatc taggaagtct gaattgacat gcaggccatc ctctgtgtag
     2761 gcagcttctt cgacatttcc accttcaagt acttcgacga ttggaaggtc aaattgtttg
     2821 gcaaattccc agtcacgttg gtcgtgggca ggcacaacca taaccgcacc tgttccataa
     2881 ctagcaagaa catagtccgc aatccagatt ggcatttcct taccattgac agggttgatg
     2941 gcataggcac ctgtccaaac accagttttt tccttagcaa ggtctgtacg aaccaagtca
     3001 gacttaaggc tggcttggtg tttatagtct gctacagctt ctgcttgctc tgaacttgtg
     3061 atagcgtcta ctaattcatg ttcaggagcc aagacagtga aagtcgcacc gaaaagtgtg
     3121 tccggacgag tagtaaagac tgtaaattcc ttgtctgttc cttttacttt gaaagttaca
     3181 ttggcaccag ttgatttacc aatccagttg cgttgcatat ccttgataga ctctgaccaa
     3241 tctagttcat ctaagtcatt gagcaagcgc tctgcgtaag ccgtgatttt gagcatccat
     3301 tggcgcattg gtttgcggac aactggatag cctccacgct cagaagttcc gtcaggaagc
     3361 acttcttcat tggcaatggc agttcccaat tcctcaaccc agtttactgg cacttcagct
     3421 tcataggcca agcctttttc gtaaagcttg gtgaaaatcc attgagtcca cttgtagtag
     3481 tttggatctg ttgtgttgac ttcacgatcc cagtcataag aaaatccaag cgcattgatt
     3541 tgacgtttga agttggcaat gttttccgct gtaaattctg ctgggtcatt accagtatcc
     3601 atagcgtatt gctctgcagg caaaccaaaa gcatcccaac ccattgggtg aagaacattg
     3661 tagccttgcg cacgtttgta acgactgagg atatcggttg cagtataacc ttctgggtgt
     3721 cctacgtgca gaccagctcc agacggataa gggaacatat caagcgcata aaacttaggt
     3781 tttgatgcat ctgttcctgt cttaaatgta tgatgttctg cccagtagcc ctgccactta
     3841 ggctcaattt ctttatgatt ataaaaactc atggtctctc tccaattttg tgatgttact
     3901 attataccat ttttgaggga atttgaaatg tgtttaaagg aaaaactgta tactgtatct
     3961 cattaatttt gatgactatg aattgctcta tctcccactt tttatcccat tgttatgcag
     4021 taaatcaagg agtaagtcaa gaagcttatc tcgtatagca caaaatactt attttagacc
     4081 gaagataaaa ctggaatgct aattaatgta ttcacaataa atagccgaca acctatgtcg
     4141 ctttttgatt tatgaagaac gtatagtgca ctgaatctga aatagtacga aacaattgct
     4201 aaaacattgt tagaaattaa ttttactttc ccaatcgatt tgttctcatc ttatttcaat
     4261 ctgctataga attcaatacg aggatcaata aaagactacc atttggaaaa cagtagtcct
     4321 tattgtttat tttgaattta cataggcatc tacagcgata atagcttgag caacatctga
     4381 aggcgaaatc ttaaacggca tctgatgaat tgtctcaccc tccatagttg cttgtttacc
     4441 aacttttatt aaatcatcat atccaacttg atccaaatgc atttctttta gagttgttgg
     4501 cataccaatt tttttgtaaa actcaatata cttatcaagt tcttctttag gtctattttc
     4561 caataatagt tgtactaaag ttccataagc tactttttca ccatgtgtta aatgatgaat
     4621 gtcacctgtc aatgcagtaa aaccattatg aattgcatgc gccgcagcta atcctccact
     4681 ttcaaaacct agaccactca ataaagtatt agcttcaaca atattttcta atgctggtgt
     4741 caccacttta gcttcacaag ctgccatagc ctgtaaacca tctgcaaaca gcgtttcttc
     4801 acatttcttc gcaattgcaa ctccagccaa tgtttgctgt tgtcccaaca tagtttttcc
     4861 atttgcctgc ataaccgcac gcgcctcaac ccaagttgct aaaccatctg caataccaga
     4921 cgctaataaa cgcttagggg cttgtgaaat aacttttgta tcaaccaaaa ctaaatctgg
     4981 atttttagaa taaaatagat aatgatcaaa tgcaccttca tctgtataaa taacagataa
     5041 agcagataca ggtgcgtcgg tcgatgcaat tgttggagca ataataacag gtttttcaat
     5101 caaatctgca atagcttttg cgctatcaat cgtctttccc ccaccaagac cgataatact
     5161 atcacaattt tctttctcag ccaaggcaac aactcgattg atttcattgt cagaagcttc
     5221 accattaaat agcgccagaa caatatggaa accataccta tgtagataat cttcaaatcg
     5281 ttttccaaca atatcataaa ccaactgatc gcataataga atcgggtaat ttcccaaatc
     5341 caaaattgat ttggcatttt caaacaaggc attttccccc tgaatatatc tagaaggact
     5401 agcaaaaatt ctcatatgta tttcctctct atctaaatgg aactactatt ttgagtaaca
     5461 aaccaatcgt cagaaaaatc atcaaccgcc ttttggatag atggcatggc gaaagctgat
     5521 tcaaaaacat ctgctcctgc tgtaacagca tgcgcacctg cagctaaagc attatttact
     5581 tgtgctacat ttttaaagga tgcagctaaa atcttactag gagagttctg tctatcaata
     5641 gcaagagcta attgacgaat gacagaattt gaatcaatgt tcagattttc cattctatta
     5701 taatatggag ctaggtaatc cgctcctgct tcgatagcta ataatccctg aataactgta
     5761 taaatagctg ttgcagtgat atggtagccc tcttttttta gcacctttat tgcacgtaat
     5821 ccagctggag taacaggtac tttgataaat atatcatctc ctgcttgtct tcgaatttca
     5881 tgagcatcct ttaagatgcc ttcaaaatct tgagaaatca cctgaacatg aatagagggt
     5941 gtagagccaa tcaattctct tacatctttg attcgttcaa aaaaattaat agaaccctct
     6001 ctttttgcaa tagtgggatt tgaagttacc ccagctagcg gcaaaatttc agaccacttt
     6061 ttaatctcat ctaaatttaa tgtgtcaagc ataaattcca taaaaagaac ctctttattt
     6121 tacaaagtat gctcagtacg tcctataatg tcatcttggg ttgccttaga aagaacattg
     6181 aagaatgcag agtatcctgc aacacgaaca attaagtctc tgtgtttttc aggatgtttc
     6241 tgagcgtcaa tcagcgtctc tctggaaaca acattgtatt gaatatggta cccatgtaaa
     6301 cgattaaaga atgttcgtaa caaagcaatt agttttaatt tatcttcttc tttggctaac
     6361 gtttgaggat ttactttctg atttaagaga accccaccta cgatttcatc tgttggtaat
     6421 tttgaaacag attttaaaac agatgtaggg ccgtgttgat ccatattatg tgatggtgaa
     6481 caaccctctg ctaacggtgt acccgcgttg cgtccatctg gagttgctaa tgttccacgt
     6541 ccctgcccta cgttggctga gatagaagat gttcctgaat aacgaattcc tccaattgga
     6601 cctcttccat aacgtgtatt aggatattta gcaatttcat caacataaat gtcataagca
     6661 gcagtaacca atttgtcagc ataatcatca tcattaccat acttaggtgc atcatgaatc
     6721 aacatttctt gaatgacctt accttcttct ccggcataat ctgtttccag tgcatgccaa
     6781 agctgacttg ggcttatacg ttcttcctca aacaccaatt ttttaattgc agctaatgaa
     6841 tccgacaaat ttgcaattcc aacttgcaat cctgatatat aatcatatac tgctccacct
     6901 tctttaaggt gttttccacg accaatacaa tcatcagtca atgctgaaca tagaatatca
     6961 ggaacttctc gttccaatga taaatcaata gaattttcaa caataacact cattcgtgtc
     7021 aaatatctta gtgttttatc ccaagcattt tctaattcag aaaagttctt catatcctta
     7081 aaatgaccaa agcttggtgc aaaccgttta cccgaagccg gatcaattcc atcattcatc
     7141 gtgataagta gaaccttagg gaagttcata taactcatac ctgtgcaacg atagccccat
     7201 ttccctggaa ctgccgtttc aacacatcca atggcactgt aatcataagc atcatcttcc
     7261 aatactcctt ttgcaataaa agaaggaata ataatctcat cattattaaa tgcaggcata
     7321 ccaaaaccaa gtttcatcac ttcaatacac tcattcatga aacgagcatc taaacctgca
     7381 tggtaacgta cagttagatt aggttgcggt agatgggttt gtgcaactga ttttaatacc
     7441 aaataagata atgggttaac agcatccttc ttatctcgag tctgtccacc aattgtaaca
     7501 ttttgatata aaggacttcc tgctgaagaa aatgtatgtg attgactgcg aaccttatta
     7561 attgtaattg tcttaatcca aagatttgtc agacgttcaa caatgctatc ttctgtttct
     7621 ttaccacttt ctaaatcagc cttcatatat ggatacatat attgatcaaa acggccatat
     7681 gaaagagagt ggccattaga ttcaatttgt aaaatacatt gaataaacca aactgattga
     7741 atagcttctg caaaagtagt tgccggttca tatgggactc tagagcaaat atctgcaatc
     7801 tcaagtaatt ctttcttacg tttaggattt gcattttcgg ctaaactttt agcaagagca
     7861 acaaagcgct ttgcatatac tttaatagca tcgattacga taaatataga gtcgtaaaaa
     7921 tgatatttat caatacttgc tggatctgtt aaatctagag ctacttttgc tttacgagcc
     7981 cgctcttcaa aacctcttaa accaaattgc aaaagtttct gatagttaac tgctaagtga
     8041 gcatctccag aattcatctt accttccata ccgaagaatc ctgtttccat ataaactgac
     8101 acttcttcag gtaataaggc accagctcta gcacgtaaat tattattttc ccaaaacgga
     8161 gcaatacttc taagttgttc ttttgtttct tctgtaatat agaaaacatc tccatcacgc
     8221 ttttcaaaaa gatccaactc attaagaaca aattctagcg tatattccgg aaaaatagga
     8281 gcatctttat tggaagaagc ttgatttccc gcaatcatag attcttcttc aatatagata
     8341 gacatatttt ccaaaatttc tttcagcata tatgcacgtt ttaggacatt aggttgttcc
     8401 ttgtatcgtt cataggcgcg tgttgctaga actgctctct cagcatcaat ataaggtttt
     8461 ttatttaaaa catcttctcg atatttgttc atcctttcag taaggctgcc aaaatattct
     8521 gtcttgattg ttgttcttgc tacttctgta tttaccatcg taagcacctt tctttcgaaa
     8581 atatttttat ttctttcgaa aatataatac cgatttttat agaaattttc aagcttttaa
     8641 ctagaaaaat tttaactctc taatttttag cacaaatgtg tgagtttaac agtaacacac
     8701 aaatacctct aagattgttt gatttcattt tggtaagcta aacgatcttg tatcttaaag
     8761 aatggaaagt aaaccactgt agatatcacc aatactaata actgagtcac aacaccttgc
     8821 catccaccca ccaaaaatcc tgatagaata gctggtgtac tccaaggcaa tgttacccct
     8881 gagaatggct gcatgaaacc tgttgcaata gctccatata ctatcacagc tgcaagtaca
     8941 ggaacaagaa tgaaaggtac aaacataact ggattcatga caatcggaaa tccaaataca
     9001 actggctcat ttacgttaaa tattgctgga aaagctgcaa cttttcctaa ggcttggtat
     9061 tgttttgatt ttgctgcaaa aagcatggca actacaagac caaacgtaat ccctgaacct
     9121 gatagaatta aaaatgaatc taaaaattgt tgagtaacaa tatgtgcacc attttctaat
     9181 gatagattac cagaggctaa catagcttta ttagcatcaa gattagataa aagcagagct
     9241 gtcactactc catttactac cgattgccca tgaacaccaa accaccacaa aaatgatata
     9301 aagaatgcaa ttccaatagc accatacaaa gatccagtta aaccttgcaa cggaacttga
     9361 atagcagaat aaatcatttc tatgaatgtt ccgccattag tcaatgactt cgctaaaata
     9421 tatacaatca tagaagataa gaaaattaca aatgctggaa tcattgcttc aaactgtttg
     9481 gcaatagctt gtggaacttg ttctggcatc ttaataacaa tttttctctt tataaagaag
     9541 gtataaatac ttcctactac caaacctata atgatagcac cgataattcc ttggcctcca
     9601 aaccaaactt tactaatagc gtccccaatc gcctcacctt gtttagggat ataagatgat
     9661 cttagcaaaa taaagaatgc agatacagat agaactccag ctggtaaagc ctctactccg
     9721 ctattcttag cataagaata ggcaattgaa aaacaagaaa ttagacccat aatagcaaaa
     9781 gttcctgaat atacttgcat aaacggctct gtccaattag ctccaaaaac actagcaatg
     9841 ctcttattta atccttcgaa cggcaattgt cccataatca agaacaaact gccaactact
     9901 gtcaatggca aaattgctaa catcccatct tttagagcta taatgccacg catattcaca
     9961 aacttcatca tcggtgcaat gattttctga acatccatct ttgacataat aaatctcctt
    10021 ttcttaccca ctaatcaaag atagggccaa atctaatact tttttcccat ctaacatacc
    10081 atagtccatc atcggaataa cagctatcgg aacatcacac ttatcacaaa tttcttttga
    10141 tttatctaat gtataagcaa cttgtggacc caatagtgca acatctatat ttggcgcata
    10201 atccgctaat ttagactgag aaaacgcctc tatttctgcc tcaactccac tagattgcgc
    10261 tgcaattttc atattattta caagcatacc agtagaaaaa cctgctgcac aaaacaaacc
    10321 aatcttcacc attatgtttt cctcctctat gttaataaca atgataatac tctagtatta
    10381 ttttttatga agttcttttc tcaaactaat aatttctttg attaaattaa tctccgtcat
    10441 actagtcatg agatgatctt gtgaatgaac aaagagagct gtaatttctg tctttgtacc
    10501 agacgcttcc tgtgccaaga attttgtttg tagattatga gcttctaata aggctgcatc
    10561 agctaactgg atttcttctt cacaccgttc acatgtacca ttttttatgt aatctaacgc
    10621 tttataaata tgttgtttcg catcaccagc atataaaatt aaacccatga taatttgatc
    10681 tggaacaata atttccataa attcctcctc atttcactat gtaaaaagct tttttcattt
    10741 gaaattttaa aattttcttt
//
LOCUS       DAVKHZ010000069        10685 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000069 DAVKHZ010000000
VERSION     DAVKHZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.069"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(46..1470)
                     /locus_tag="VWW99_001694"
     CDS             complement(46..1470)
                     /locus_tag="VWW99_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9539503.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     SLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2015..4294)
                     /gene="pulA"
                     /locus_tag="VWW99_001695"
     CDS             complement(2015..4294)
                     /gene="pulA"
                     /locus_tag="VWW99_001695"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9539504.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGCNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4406..6364)
                     /gene="ligA"
                     /locus_tag="VWW99_001696"
     CDS             complement(4406..6364)
                     /gene="ligA"
                     /locus_tag="VWW99_001696"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9539505.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLNYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6457..7635)
                     /locus_tag="VWW99_001697"
     CDS             complement(6457..7635)
                     /locus_tag="VWW99_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9539506.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7715..8578)
                     /locus_tag="VWW99_001698"
     CDS             complement(7715..8578)
                     /locus_tag="VWW99_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9539507.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8581..10482)
                     /locus_tag="VWW99_001699"
     CDS             complement(8581..10482)
                     /locus_tag="VWW99_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9539508.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGDELDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQTELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3204 a         2356 c         1909 g         3216 t
ORIGIN      
        1 tagcaaaaaa ataaaaccag gaaaacaatt tcctgatttt acacgttatt tcacatcaaa
       61 aacaatggat ttgacatttg tcatcgcttc aatgctatat ttaattcctt gcactccagc
      121 tccagaacct ttgacaccaa ggaatgggaa attatctgga ccacgctggg ttttattatt
      181 aatgtggact gtacctactt caagtttttc agcaatttca aaggcttttt tgaaatcatt
      241 tgtaaagact gatgattgaa ggccgaattc agattcgttg gcaaaggcaa tagcttcctc
      301 tacactagcc acacgaatga ttggtaaaac aggaccaaat ggctcttccc atgccacttt
      361 catatctttt gtaacttggt caaaaagcac tggccagaga agattgccct cacgtttgat
      421 tggtgtaaga gcctgagctt ctttttcttg tgcatcctca atcaagctcc aaatgaagtc
      481 ggctgaagca ttgtcaataa caggtgtaat atcagcattg tcaaatggat caccaactgt
      541 taatttagaa acttcttcct gaagcaaagt agctaattta tctgctacac tttcgagaac
      601 aatgacacgt ttaatggccg tgcaacgttg tcctgagtag ctaaaggctc ccgcaacaat
      661 ttgcttggca gcatgttcca aatctgcatc ttctagtaca agagctgcat ctttcccacc
      721 aagttccaac atgataggac gcataccagc taaacgacca atacgttctc caataggagt
      781 tgaacctgta aagttgatga agttgacttc tttgtgctca atgatataat ccccaatttc
      841 tgaaccacga cctgtaatgg tgttgaaaac acctgccgga atccctgctt cttcaaatgc
      901 tttagccaac aagagtccag aaatggaacc ttgtgttggt ggcttaaaca tgaccacatt
      961 ccctgcaatc aaggcaggtg caattttaga agcagataaa ttaactggat aattaaaggg
     1021 agcaatcgct agcacgatac caactggttc acgacggaca acagccagtt tgtttttact
     1081 tgttgcctca aaaccaccac cttccattgc ttgtccagtg atacggagac cttcctcagc
     1141 agcataacga atcaagtctg ctgtacgcac tacttctcca attgctgctt taatcccttt
     1201 tgctacttct ttggcaagga tagtaccaat ttcttcctta tcgcgttcta aaatagctgc
     1261 tgttttatgc aaataagccg cacgttcaac tgctgataaa gctcgccatg ctggcagggc
     1321 tgcacgcgca gcttgcatag cctcatcagc ttcagtctga gtcatggctg gaactgtacc
     1381 caattcttct tgattgattg gtgaataaat cgtaatttct tgttcagatg atttccattt
     1441 tccatttact aaattctgat atcttgtcaa atttttgtcc tcctgaaaat aattaagata
     1501 tattttacca aattttcaga aaatcacaac cctttttgca aaatttttga gattattttc
     1561 acaaacttga tttttcaaag tatactcaat aaaaattaaa aaaatccact acgtcaaggc
     1621 gaggctaatg tggtttgaag aaattttcga agagcgtgaa tgagtatcat ctatagtaaa
     1681 ataaaaaaac tgaacaattt ggttggggac agccaaacca atttctcaca atgtttcaga
     1741 aacaagggtg tgctattcca atttcagcct actataactg tcatagattg ctgaaacaaa
     1801 gtctaggtaa aagtcttcat aataaaaaga cctcctatca agtgttcaaa aactttgata
     1861 ggaggtcttg ttttgtgaaa atatttatca aattttctat acaagtgagc tgttagccag
     1921 gttctttcta ttctttcaat ttcaatgaat ggatttttta ctaatactca taactgggaa
     1981 tttgtctgtg taaaaatagc gagatagatg gtatttataa aacactcaag acagctagac
     2041 taatatcatt taaaacatta tcttcttttg agcgactgtt ggttaccaac atagctaaat
     2101 ttcctgcatt ttcaaattga tagggttctg atttagcatt cacaaccacc aagaggtgtt
     2161 ctttgccgtg aacttcatag ataaggtagc cgctatgttc aatcgcagaa tgcacaaaga
     2221 catgatggta aatttcatca tagctagagt aagaaaaggc accagttttt gtcttcaatc
     2281 ggatgacttg acggataaac tcaatactgt cttgacgctc attaatcaag ttccagttca
     2341 cttggttcac actgtcagga gcattatagc tattcatcgc acgctctcta tcatcatggg
     2401 tcaactcacc attttcacca gtcgcaacca gtttggtacg accaaattct tgaccgattt
     2461 ccataaaggc catcccctgc atgagcagat tcatggctgt ggcagtttcg accttgcgca
     2521 tgatttgctc tgaactttgg tctggatgaa gggttgccaa taaatcgtga agattgtaat
     2581 tgtcatgggc ttctacatag ttaagcacct gatttggatg tgtatagctt cctaattcac
     2641 gacttcctag gattgcttta gctagaattg gctctgtcgc agcaccactg acaaaacctg
     2701 acttgatagc accataaact tctccccctt tgacagcatc gcgctgattg tcattaaaga
     2761 aaccaatatt tggcatctgg taggcattgt ccttcttggc cttatcataa ggggcaagac
     2821 ctgttcccat atcccatcct tctccataga ggataatgtt ggagtcgatt tcatccaagc
     2881 tttgacgaat catctgcatg gtcttgacat catgaatccc catcaagtca aaacggaagc
     2941 cgtcaatatt atattcctgc acccagtata gaagagaatc aatcatatac ttgcgaaaca
     3001 tttcgtgttc actggctgtt tcatttccaa cacccgttcc attctggaag gtaccatctg
     3061 gattcatacg ataatagtaa tcagggactg ttgtttggaa tggtgcatca acaactgaga
     3121 aggtatggtt atagactaca tccataatga ctccaatacc cgcatcgtga taagcttgaa
     3181 ccatcacctt caaatcacga atgacctgag ctggatcatc tggattagtt gaaaaactag
     3241 tttctggcgc gttatagttt tgtggatcat aaccccagtt gtaggttaca tttccatcct
     3301 catcgtattc tttatgacgg tctgcaattg gttgcaattg aacataattg cagcccagct
     3361 tcttgatgta atcaaaagca gttgactggc cgtattggtt aactgttcca gcctgagcag
     3421 cacccaagaa agttcctcga agatgttcat ctacacccga tgtaggtgat ttagtcaaat
     3481 cacgaatatg catttcacag ataactgcct tacatggatt ttccaagcgc caagtagcct
     3541 ccgaaccgtg cttaacctcg aagttttcaa cttgcttttc tacatggctc agaatagctg
     3601 aacgtttgcc atcagggctg gtcgcgattg tataaggatc acgtgtcagt gtttggtgat
     3661 gagggaattg gacttgatac tgataagcct tacctaccaa atcttcctca acatccaaac
     3721 tccagacacc gattgtattg tccttatgat tataagagta gctattgcct cttttcatct
     3781 caaaagtctt ccaaacgggt gcatcattag cagctgattc ataaacgaca acttgcactt
     3841 ctgtcgctgt aggtgaccag agggaaaaat gagcctgatt gtcctctaca cggcaaccca
     3901 attctccttg gtaaccccaa tgatggtcaa aactagcact gttaatggcc ttatcaaagg
     3961 caaaaggatt ttgattttta tagaaaggac tggcaatagc aggattttca gagtaataaa
     4021 tcctatcatc gccttccaaa atccagacct ctgttaatag gggatagtga ttaaaacgga
     4081 tagaatattc tttactagtt tgacctgtat gaaccacaaa attcaagctt tctataacat
     4141 gtgaacttgg gtgttcaaag ctaaataaag ctccaaaata atcttctttg taggttagca
     4201 aatcaattcg ttgatcctga ctttttacaa aggagcaagt gtcatattct ccattcttac
     4261 gatggtaatg aatgcgcata gggtagttat acatttttta tttttccttt ttactttgtt
     4321 tctatttcac taataaattt ttgtcaatct cgtctcaatt aacagacata gtcatattct
     4381 ctaaactctg tttttaaacg atccattaca aactttctag ccatgcctca tctctgacct
     4441 ggataccaag ttcttgtgct ttttgcagtt tacttccagc gtctgcacct accacgacga
     4501 ggtcggtctt tttagaaaca ctacctgtca ctttggcacc cagactttcg agtttacttt
     4561 tagcttctga gcgcttgagt cgttccaatt ttcctgtcaa taccacggtc aaacctgaca
     4621 aggccgcatc cgctactacc gtctgtcctt tatagttcag attgacccca gtttctttca
     4681 attctctgag cagaatttca gagccttctg tcgcaaaata agtctgaaga cttttggcaa
     4741 tcacgccacc tagactttca atactagcca cttcctctga atctgcctga tacagatttt
     4801 caattgaatg gaaatattga agtaaaagct gactagcctt gcttccaaca tgacgaattc
     4861 ccaaaccaaa taagagcttc tcggcagaat tttcctttga tgcttggata gcctgataca
     4921 gtttagcagc ggacttttcc ttaaccccct ctaaaaggag gaaatcctct tcttgcaaac
     4981 gataaatatc cgccacatcc ttgactaaat tagcagcaaa aagcttctca acaatagatg
     5041 gaccaaggcc tgtaatattc atagcatcac gagaagcaaa gtgaatcaag ccttccatga
     5101 tttgagcagg gcaacgcgga ttgatacaac gtagggccac ttcatcttca aagtgcaaca
     5161 agtcagagtt acaacttgga cagtttgtag ggatatctag tttttcttca gaaacccgtt
     5221 tggactctac cacacgtaaa acggcaggga tgatgtcacc agccttatat acaatgaccg
     5281 tatcgtcttt tcggatatct ttttcagcaa tataatctac attgtgcagg gtcgcacggc
     5341 taacagtcgt accggcaagt tgtactggtg ttagattagc agttggagtt acaacaccgg
     5401 tacggccaac tgtccagtca actgataaga gttgagcttc tttttcttca gcagggaact
     5461 tgtaggctac tgcccacttt ggagccttaa cggtaaaacc aagttcttct tgacttgcta
     5521 ggtcgttgac cttgattacc actccatcaa tatcgtaagg cagattttcc cgttcctgtc
     5581 ctacttcttg gataaaattc cagatttcat ctatgttttc agccaagatt cgcttaggat
     5641 tgaccacaaa acctagttgt tctaggtact tcaaaccctt ttcttggcta tcacgagttg
     5701 aagggctggc ttcttgatag agaaacgttg caagattacg cttggcaact actgctgtat
     5761 ccaactgacg cagagttcct gctgccgcat tacgaggatt agcaaattca ggctctccat
     5821 tttcttggcg cacttggtta acttggtcaa aggaagcgcg tggcatgtaa cattccccac
     5881 gaactgtgat atctagttct tctggcaaag tcaaagggat gtccttaaca cgcttgaggt
     5941 tttctgtgat attttcacca attgaaccat ctccacgtgt taccccagca accaaaatcc
     6001 ccttttcata agtcagcgag atagataagc catcgatttt cagctcacaa atataggtcg
     6061 gatgagccac ttccttacga acacgcgcat caaaagcatc tagctcctca cgtgaaaaag
     6121 catcctgcaa actataaaga ggatactgat gactgtattt ttcaaaacca tctaaaacct
     6181 tgccaccaac acgatgagtc ggactgtctg ctagcacttg ctctggataa gcagtttcta
     6241 actcgaccaa ctcacggtaa aggcggtcat actcactgtc tgaaaccgag ggattatcgc
     6301 tggtatagta ctcagtcgca tagcgattga gcaaagcgac taactcattc attcttttat
     6361 tcataagacc attttaccat aaaacaagct ctcctcacaa acgagaaggg cggaaaaaac
     6421 acttagtttg aaattatttt tgaaactcaa gcaaccttat atcaattttt caaaatgagt
     6481 tcgaacataa ataaacgata tacaagacaa gatgataaca ccacttccaa ttatcaggaa
     6541 agaagagaga tgtacacttg gcaagactgt cataaatcct tttgcaatag gcataaatag
     6601 aatagctaag gtaaaaattg tactcagtac tcttccaaga aattcgctct caaccttggt
     6661 ttgtacttga gtaaaaaagt gaatattaaa aatcgtcata aacaattcac aaactaaatt
     6721 tccagaaaag gaaagaaaag ttggaagtgg taatcccatc ataaaaactc cgacacctgt
     6781 caaagccagt aaaatcaaaa gattataaat attagcttta attttactag ctagaagagc
     6841 cccaatgatg gaaccaatag cccccatagt taaaatactt gcataggctc cttctgaccc
     6901 gtaaagctga ttcgaaaagg gaagtagaaa ttcaaaagct gcaaaaaaga aattaacgct
     6961 ggaagctacc agcaaaagga agaaaatttc ttgctgatgc cagatatagt gtaacccatc
     7021 cttgatatct acaaaaatat ctctcccagt aaaagccttt ttctcttgaa cttttgcttc
     7081 ctcttttgga aggaaagcca ctagaacaaa agcaatgaaa aaagtcagcg agtctagcag
     7141 tagcgtcata tggagacttg caaactgtaa aacaaggaag gaaagaacag gagagctaac
     7201 acctacaacc tgcaaaacca gctctaagcg agaattatag atcacaatct catctttctc
     7261 caccacttca gttatgatag ctttattggc tgtgcgagaa aaggcaaaag caatagcctg
     7321 cacaatgtta gcaacaatca aagcgccaat catccagcta tcattcctta tgaaagaaat
     7381 agccagacaa agaatcccac aaacaagatc tgccgtcatt aaaatcttac gacgagaaaa
     7441 acggtctgaa ataactccgc caaagggatt gacgagaata gatgtgacga gctcagaaat
     7501 ctgatacatt cctaaaactg tctgtcctat agtccccata gaagccaacc agacactatt
     7561 tccataatca tagagcatat ttcccatttt attgatagcc ccacggctaa tcaactgcac
     7621 tgcatagcga ttcatattaa agctcctctc aaattttgaa actattgtat caaaaccgaa
     7681 aggagctttt tattttttcc cttatttggg aaaattaact tttgacaaat ttttcgtagt
     7741 gttcctgata ataggctact tgctctggaa gacctaacac atcaaaaata tgcatggcct
     7801 cttgcatctg cttacagcct tctttacact gtcctttttg atataaggca aaacctttta
     7861 aataatggaa aacattacgc tcataaagct taataccttt gtcaataatc ttctctgtat
     7921 aagcctcaaa atagttggca ttataaaaag aagaatgctc taaacaatgc tggtaacaat
     7981 tgagggccaa aattaacact aatctcttat ggcgactaat ctcttggtaa aattcctccc
     8041 tctccataac ttctctacca atccgagtga catagtctac atcgtagaaa ctatagaggt
     8101 taccgaaaag aatcaactca tacatggtcc attcttctgt tttgaagaga taatctgcta
     8161 ccttatccaa atcatcctgc ttcatatcat aactcgcatc tctttgacaa atcagacctt
     8221 gtagcaaaat ccagttcagc tcaaaataaa ggggagtcgt cgaactctta gacttttcaa
     8281 gttgttctct ttgaagcttt tgaaaacctg caatatcgtt tgaatagtaa agtgggataa
     8341 tctgtgccat catagacaca tgttcatgat tatgaaaatt ccttgcctta tccatgaaat
     8401 tttcgattgt tacatgaatg ttatccaaaa tctcaaagaa acgggagact gccaggtcag
     8461 actccccaag ctcaaagcga gataactgag aggtagagca ggattcgcct gctgcttcct
     8521 ttaaagaata atttccactt gttcgaaatt cacgaaatac ttttccaaga tgttccatct
     8581 ttacacctgc tctgataatt cttcccactc aagcatagct tcttcctgac gatggctgat
     8641 tttgtccagc tcagtctgta attccatgag tttgtcggca tcgtttgttt ccaacatttg
     8701 ttcagaaatg gcttggcttt gactttctag ctcttcaatt tcagcttcta gactttcgat
     8761 ttgtcgcatg agtttgcgaa cttctttttg actttctttc tgggcctgat agtcattgac
     8821 tggacttgct tcctttgctt gattgctagt tgaagcttcc tcagtctgac tcatttctgc
     8881 tgttgctttc ttctcaacat agtagtcgta atctccaagg tagagagttg aaccattctc
     8941 agacaattcc aaaacatgag ttgccacacg attgataaag taacgatcat gactgacaaa
     9001 aagcaaggta ccatcaaagt caatcaaggc attttctagc acttccttac tatcaatatc
     9061 caagtggttg gtcggctcat ccagaaccaa aaagttattg ttttccatag acaatttagc
     9121 taaaagcaaa cgagcttttt cgccaccaga tagcatgccg actgattttt taacatcatc
     9181 tcctgagaaa aggaaggctc caagacggtt gcggatttca acttctggtg tcagtttgaa
     9241 atcattccag agttcatcca gcaccgtatt acttggtgtc agcttgcttt gggtttggtc
     9301 atagtaacca acctcaacat tagcgccaaa gcgcttttct cccttgataa aaggaatctg
     9361 gtccacaata gacttgataa aggttgactt gccgatacca tttggaccaa cgatagcgac
     9421 agcattcatc ttacgaagat ctaggttgat aggttgtgac aagacttccc cgtcataacc
     9481 aatagcagta ttttcaacag tcaaaacaac attgcccgac gttttttcag actggaaggt
     9541 catgttggct gctttcttgc cagcttcagg cttgtccaaa cgctccattt tttctagttg
     9601 tttacggcga gattgagcac gtttagtcgt tgaagcacga actagattgc gattgacaaa
     9661 gtcttccaga gcagcgattt ccttctgttg cttttcatag ttttttgcct cagtagctag
     9721 cttttgctcc ttcaattcga caaaacgaga gtaattcccc acatagcgat ccaaggaatg
     9781 cttggtcaaa tctagcgtaa ttgtcgcaac cttgtccaag aaataacggt cgtggctgac
     9841 gataatgagg gcaccgctat agtttaccaa gtaattctct agccaggcga tggtttcaat
     9901 atccaagtgg ttagttggct cgtccaagac caagagattg ggcttttcaa ggagcatttt
     9961 ggcaagtgcc aaacgagtat tttgaccacc agaaagctca gcaattttca tctgccacat
    10021 agactcgtca aacttgaatc cattcaaaat cgctcgaata tcagcttcat aggtaaagcc
    10081 gcctgcttgg cgaaaattct cagataagtg gtcgtaatct gacatcagtt tatccaattc
    10141 atcgccagac ttttcaccca tctccaactc catctgacgc agttgtctct ccgtccgacg
    10201 caaattatta aagacatgaa gcatttcatc gtagatggta ttttcagact caaaacggct
    10261 atcttgggct aggtaagaca gagaaatatc ttttttctta ttgatttctc cgctagttgg
    10321 ctcctcttct ccaactaaaa tcttcaaaag agtagactta cctgcaccat ttttcccaac
    10381 aagagcaatc cgatctcgtt catcaacctg caggttgata ttatcgaaaa gaacctctcc
    10441 tgcaaaagaa cgttcaattt tattagcttg taaaataatc ataagagtag tatagcatgt
    10501 ttccctaagg cattcaagat aatcgtaagt cttttagtac aacttttata acataaaata
    10561 aactaaatta tgtatatttt atattagatt ccttcactat cttgttggat tttctaacca
    10621 gctaatcttg tttcaaatag ttatcgcaca agtctattat ttaattcttt tcattattta
    10681 cgtac
//
LOCUS       DAVKHZ010000070        10664 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000070 DAVKHZ010000000
VERSION     DAVKHZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.070"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(418..1311)
                     /locus_tag="VWW99_001700"
     CDS             complement(418..1311)
                     /locus_tag="VWW99_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539509.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(2065..2295)
                     /gene="blpU"
                     /locus_tag="VWW99_001701"
     CDS             complement(2065..2295)
                     /gene="blpU"
                     /locus_tag="VWW99_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9539510.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            complement(2498..2788)
                     /locus_tag="VWW99_001702"
                     /pseudo
     CDS             complement(2498..2788)
                     /locus_tag="VWW99_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2834..3067)
                     /locus_tag="VWW99_001703"
     CDS             complement(2834..3067)
                     /locus_tag="VWW99_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9539511.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(3073..4065)
                     /gene="plsX"
                     /locus_tag="VWW99_001704"
     CDS             complement(3073..4065)
                     /gene="plsX"
                     /locus_tag="VWW99_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9539512.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFVVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(4062..4832)
                     /gene="recO"
                     /locus_tag="VWW99_001705"
     CDS             complement(4062..4832)
                     /gene="recO"
                     /locus_tag="VWW99_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9539513.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(4829..5998)
                     /locus_tag="VWW99_001706"
     CDS             complement(4829..5998)
                     /locus_tag="VWW99_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9539514.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6147..7157
                     /locus_tag="VWW99_001707"
     CDS             6147..7157
                     /locus_tag="VWW99_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9539515.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            complement(7348..7785)
                     /locus_tag="VWW99_001708"
     CDS             complement(7348..7785)
                     /locus_tag="VWW99_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9539516.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(7871..10504)
                     /gene="polA"
                     /locus_tag="VWW99_001709"
     CDS             complement(7871..10504)
                     /gene="polA"
                     /locus_tag="VWW99_001709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9539517.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEIKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         3092 a         2592 c         1768 g         3212 t
ORIGIN      
        1 catatctata ataaataaat tactactaca ttttcctaga ttatcaaatt acttttatat
       61 cactaagact caataaaaga taatgtctat ttaactttta tacgattact acctcttttt
      121 agtttacact tcctacatca taaataaaca tactcaaccc tcacaatctc caaataactc
      181 ttctatatca ttgaaaattg agtttcatcc tctatactaa tgtaggacat tcctttaatc
      241 caatataaga ataccttgtt ccattaatct ctacccgata ttatagatag aaccaaattc
      301 ctatacttaa aaggacaaat aacgttccaa ccataataaa cctcctttct ttttgagaac
      361 gttttcttta taggtttagt atagcaaaaa catgaaaaaa acaacatgac ttagaagtca
      421 ggttgttggg aaatctatct gtttttctgc taaaattctc tgaactaata cctgatcgtc
      481 tagcaattct gctagagaaa tcgccttttc atagaaactt tctgcctcta aaatattctt
      541 gtctacaaat aaagcatatt ttgcctttag caaaaataga tgaggtcggt aaaaaacatg
      601 tcttgtttta gcaataattt tctcaaatgt ttgaatatat atcagactat cttctgtctg
      661 aatttggata aataggctca gaagaacttt ttccatttga actaggctag ccttatcttc
      721 atattctgaa catagcaata ctttttgagc taactcctca aaatgttctt tatcttctaa
      781 tcccatagca caacaaaaga aatacaattc aataatcaac aaatcattta cagaaaaata
      841 tttctttttt aataactgct ggaaatattc ttccactaac cctaaaccgt aggtcacatc
      901 tgaactacca taaatatcaa aacgagcttg tgtcacatcc acaattaact gctcttcctc
      961 aggcaaattc tcataatagt tttcatatat ttcctcaagt atagcttctt ttcgttcgat
     1021 tcttccctta tcagcgtagg tttgagaatg aatcagctca tctttgagtt ttaggaatcc
     1081 ttttggaatc tctataacct taacatccac aatattctgt aatgatactc caagtttatc
     1141 cgcaatcaat aataatttag gaagtgttgc taaagcttgc ccattttcaa tacgtgctag
     1201 ctgacgaatc gtcaactctg attcatcacc acaaatatct atctgagttt ttccacaaga
     1261 tcgcctaata gttttgattt tataaccaat ttcacgtttc aactcatcca tagtaatact
     1321 ccttaagttg attacaaaga tttctttttc ttcctaattc ttttgtcaat catatataaa
     1381 acgataagcg aaaacagcgg tagaacaata taactctttt cactcattat cagctgaaat
     1441 cccagggttg ctagtatctt aaagatgaaa atattcatca aaaatagaaa aacaaatact
     1501 aaaaaatatt ttaaaacctt cttcatttct tttcttcaac tttcctaatt tctacaccta
     1561 ttttatcgaa aatatatata aaactccaaa taaacacttc gactgcactt tttttggcaa
     1621 aaaatagaca gaacaaacat cctacctact aaaaatatga tatgaaagga aaatgatttt
     1681 ttactgacac cctaggcaag cttgggacaa ataaatcatt gatagcaaca tttacgacat
     1741 cctatttaaa gttccttcaa tttctaatgt ccccgctatt tctaagccac ctctggtatg
     1801 atctattcct agtcagaata gataacaccg ttctaaatta cgattcatag ttactccttt
     1861 tttgactcgt tactataaga ctcctattta catgcgcaaa ataattttga ttcagattca
     1921 tcatttatag ttttgattat tttttaacag atttttctgt tttcttctcc gaagacttta
     1981 aaaatttttt tctaattaaa tcatccatat aaggaccaaa tataccaact actaaaccaa
     2041 taataaaact tttaaaatcc ataattacca ccaacatgtc gctgcatagg ccacacctcc
     2101 aagtatagct cctcccacag caccagttgc tgcaccttgc catgttcttg ttttaattcc
     2161 tagctgaaga cctcgtactg ctcctcctcc aacacctgct ttggcaaaat ctccccaatt
     2221 gcatccgcca ccttcaacgc aagcaagcat ctcagtatcc ataatttcaa attgtgacat
     2281 cttttttgta ttcataacga atacctcttt tttattttta atatttgtct tgttacaaac
     2341 ttgacaagtt tagtataaca gtatctatta atttttttca tccaaatctt gaattggcat
     2401 cgaaacgtct tgaattagct tttttgtttc aaaatcatct ctatttttaa aaaagatgtt
     2461 ttctaatcac ttttttacta ttagacttcc tgcaaaacta gaatcctagt tcatgattga
     2521 taataccagc aatcaaattc attcgtaatc cgaagcgttt acgatgattt cgataggttg
     2581 ttgaaaacat tttaaacgtt tttactttgg caaagatatt ctcaaccttg cttctctcct
     2641 tagatagcgc atgattacag gctttatctt caactgttag cggcttgagt ttgcttgatt
     2701 tacgtggagt ttgtgcttga ggatatatct tcatgagccc ttgataacca ctgtcagcca
     2761 agatttttcc agcttgtccg atatttctca tttctaaaaa ccatctactt ccgttgactt
     2821 ggaactccga ttgctatttt ccttgaatga ttttaaccac atctcctacg ttttggagtt
     2881 ggtcaatttc ttcatcgctg atttcgatac taaattcatc ttccagagtc aagataaact
     2941 ccatcaagtc aacagaatcc gcatccaaat cgtctttcag actcaaggat tctgtcacga
     3001 caaagtcctc tccctgtcgc tcttggataa tggtcacaat acggtcaaaa atttcttttt
     3061 ctgtcatctc ttttattctc ctgaaaattc acgcgcagtc tgggcaacca cgtctgtttc
     3121 tagcatggta cggatctgac gaatcgtact ataaacagcc ttggcatcgc ttgagccatg
     3181 agtcttgaca acaggtgcct taacaccaaa caagaccgct ccaccaacat ctgaataatt
     3241 gagctgtttt ttcaaacctc tgaggctgtc cttgagaagg agggcaccta gtttcgctcg
     3301 aagaccacca cctgtaatag ctgtcttgag caagcccatg attcccatag ctgtcccttc
     3361 gatggatttg agcacagcgt ttcccgtgaa accatctgcc acaacaacat ctgcaacgcc
     3421 attcatcaaa tcacgcgctt ccacgtttcc gataaagttc aaactttcat cagccgccag
     3481 taattcataa gtttccttac gaagcgggtc gcccttgcta ctctctgttc cgttgttgag
     3541 caaaccaacg cgtggttgcg caatgccacg gacattttta gcatagaagg aacctagaac
     3601 cgcatattga tggaggtgct gggctgtatt ttctgcattg gcaccaaggt ctagcatgtc
     3661 aaaacctttt ccatcaacgg taggcaaggt agacatgagt ccaggacggt cgatattctt
     3721 gatacgaccc acgacgaaga atcctgctgc caacaaggca cctgtattcc cagccgaaag
     3781 gacagcgtct gcttcaccat ctttgacagc cttggctgcc aataccatac tggcattttt
     3841 cttattccta atagctctcg taggttcatc atccgaatca atcttctcat ccgtatggat
     3901 aatgctgacg cgctctgtcg ctgtcagata ttgcttgatt ttagcttcat ctccgtaaag
     3961 ttgaacctcg atatctgaaa agtcagatag ggcttgattg acaccctcaa caatggcctg
     4021 aggtgcgtaa tcgcccccca tggcatctac tgcgattttt ttcatttctt ttcctctttt
     4081 agtaattgtc cccagtctgc tagggaatca ataaatttct ttgattttag gtgaatccca
     4141 acgtactctt catataattg atccataaat tggcgtagct cttgcttgat tccaggcttg
     4201 agcgaaatgg tctccaaagt ctcaaaatca atagcttgaa attgattgag cagatagggg
     4261 atattgggat tgagatgaca acgtctctta tcctcatgat aatgctctgg acagaggcag
     4321 gctccatatt tgaaagaaaa gtcaaaagcc tgaccaaccc gatggcagaa gacacactca
     4381 ttaaaattga ggctgattcc aaatcgagtc aaaatttgaa tttcaaaaat attggtcaaa
     4441 acctgataat ccaagcctgc ttccatcaac tccaaagtct tttgcaaaaa agcaaacaag
     4501 ggagcatcct gctgattgtc ctgcaaacta gcatctgcaa gagctgccac ataggtcgca
     4561 taggccatga caaagaggtc actattaatc ttgggaaaag tcatgacctc atgatagtct
     4621 tcgatgtaac tgagtccgtc atcattgatt cgcaagagaa atcgtgccag caccaagggc
     4681 tgaataacag gcgccagctt agactgacca gcgtgtttga caaaaaacat gcgtttgcca
     4741 acctgctctg taaaaatttt gacgagcttg tcatcctcac gaaaattgcg attgtaaagc
     4801 accaagcctt gactcgtgat agactgaatc atgcttctct catgtactcc tcaagtcgtt
     4861 tcatggcttc tttgatagtc tccatgctgg ctgcataaga taggcggacg tagccttccc
     4921 cgtaacgtcc aaaggctgca ccagggataa aggcaacggc cttcttctga gcaaaatcct
     4981 tcagaaaagc aaaggagtct tgattgtagc ccgctggaat tttagcaaaa atatagaagg
     5041 caccgtctgg tttgataatc tcaaaaccaa gagcagtcat tttttcgatg atatagtccc
     5101 gacgctggat atattccttc ttcatgggct ccgcatcatt tttaccagcc gtcaaggctt
     5161 ctaccgcagc atgttgcgcc atggtatttg cggcagtgac caagtactgg tgactcttga
     5221 ttaactgggc tgtgaaggtc gcaggagcga aaatcagccc caaacgccaa cctgtcatgg
     5281 catgcgattt agacaaacca ttgataataa tagcctggtc tctcaacatc gttcctagag
     5341 acacatgggc ttcgcctgtg taggtcaatt ctgagtaaac ctcatcacag acaacaaaaa
     5401 cttcgtactt gcgtaaaaca gctgccaagg cctctaactg ctctcgactg taggtaattc
     5461 ctgtcggatt ggctggatag ttgagaataa ccgccttgag cttatcaccc tgctccaaaa
     5521 tggccttctc caacatctca ggagtcaaga caaaaccatt ttcagtcgta tcaatctcaa
     5581 caatttctgc cccaactaag ttaacaatcg gttcataacc cggataagca ggagctggca
     5641 aaagtacctt gtctccctct tccaaaatag ccgtcaaagt cgcagataaa gcctctgtcg
     5701 ccccaattgt aaccaagatt tcattttcag gagcatagtc cagttggtac ttttccttaa
     5761 caaagtcact ggctgcctga cgtagagtca gcagaccact catccctgta tagtaggatt
     5821 ggttctgatc aatcgctcgc ttggccgcct ccttgacatg gtctggcgtt gtaaaatcag
     5881 gttcccccaa ggtcaaacgc aagaccccag gaatctccga aataacctgg tcaaactgac
     5941 gaatcaacga aacttgaatt ttatctaact gtttattaaa gcgcttagtt aagtccatag
     6001 atacctccta ctttcaaaac gtatctctat tatactacaa aagccccccg accgcaaaaa
     6061 tacattagag aatagctttc cactttctat tttacttttc ctgtttatag gtctataatg
     6121 gtaacagatt ctatttggag gtttttatgt catttctatc aaaaaatgga gcaggtatct
     6181 tggcctgcct tctcatttcc atcctatctt ggtacttagg aggattcttc cctgtgattg
     6241 gcgcgcccgt ttttgccatt ttcataggca tgctcctaca tccctttctc tcgtcctata
     6301 aacaactgga tgctggtttg acctttagtt ccaagaagtt gctccaatat gccgttgtct
     6361 tgcttggttt tggtctcaat atctcgcagg tcttcgcagt tggccaatct tcactccctg
     6421 tcatcctgtc cactatctca atagctctga ttattgccta cctcttccag cgtttctttg
     6481 cactggatac aaaactggct accttggttg gagtaggttc ttctatctgt gggggttctg
     6541 ccattgcagc gacagcgcct gttatccatg ctaaggaaaa ggaagtagcc caagccattt
     6601 ccgttatctt tttcttcaat gtcttggctg cgctcatctt tccaaccctc ggcacctggc
     6661 ttcatctatc caatgaaggc ttcgccctct ttgcagggac tgcggtcaac gacacttcct
     6721 ctgtaacggc tgccgccagc gcttgggaca gtctttacca aagcaatacc ctcgagtctg
     6781 caaccattgt taaactcaca cgtactttgg ccattatccc tatcacgctc tttctatcct
     6841 actggcaaag tcgccaacaa gaaaacaagc aaagcctaca actgaaaaaa gtcttcccac
     6901 tttttatcct ttactttatc cttgcctctc tcctcactac actactcacc tctctaggtg
     6961 tgtccagtag tttctttacc cctctcaaac aactctctaa attccttatt gtcatggcca
     7021 tgagtgctat cggtctcaaa accaatctgg tcgctatggt caaatccagt ggaaaatcca
     7081 ttcttcttgg agccatctgc tggattgcca tcatcctcac cactcttggt atgcagaccc
     7141 ttatcggcat tttctaacac tcttcgaaaa tctcttcaaa ccacgtcaac gtcgccttgc
     7201 cgtatatatg ttactgactt cgtcagttct atctgcaacc tcaaaacggt gttttgagca
     7261 gcccgcggct agtttcctag tttgctagtg tgctctttga ttttcattga gtataacaca
     7321 aaaggtagcc catcagctac ctttttctta tgcttcctca atcaagcgag tatgttctct
     7381 cttgatacag cgattcatca cgatatcatc acatccacca tcacgcaaaa tctctttcgc
     7441 ttctaaactt tcaagtccta gctgtgccca aaaaatcttg gcatcagctt tgagaaaatc
     7501 acgcgccaca tcgggcagaa attcactgcg acgataaaca ttgacaatat ctacaggaaa
     7561 aggaatttca gcgaggctag cataagcctt ttcacccaag atttcgccac ctgccgcctt
     7621 gggattgact gggatgattt tatagccccg agcctgcatt tcctttgtta ctcgattgct
     7681 ggttgtttct tcacggtcag acaaacccac cacagcaagg gttttactcg ttgcgagata
     7741 ctgacgaatc acaccatcac ttggattgat aaattcttga ctcatagaaa acctcctttt
     7801 tcatcagtat agcacatttt gaaaaggttt gcagaattct actaaataaa aaggaggact
     7861 agcccccttt ttatttagcc tcgtaccagg ttgccccttc attctcatct gcgataagag
     7921 gaacactgag ttgaatggct tcttccatgg tttgtttcac caattttttt atctctacca
     7981 attcagattt aggcacttca aggacgattt catcgtgcac ttgtaacagc atcttagtct
     8041 gataaccacc tgcaaccaag gctttatcca gctgaatcat ggcaatcttg agaatatctg
     8101 ctgccgaacc ctggataggt gagttgatag cagttcgctc cgcaaaacca cgaatattga
     8161 agttgcgcga attgatatct ggcaactcac ggcgacgctt aaagagggtc tctacatagc
     8221 ccttatcacg cgcctcccgc accacttcat ccatgtagtt tttaatacct ggaaaacgtt
     8281 caaagtaggt atcaatgtag gctttggctt ccttacgact aattcccaaa ttattagaca
     8341 agccaaagtc tgaaatccca taaaccactc caaagttaac tgccttggca ttgcgacggt
     8401 catttgcagt cacattatca ggacgctcaa tgccaaagac ccgcatggct gtcgaagtat
     8461 ggatatctgc cccctcttgg aaggccttaa tcaagtgctc atccttagaa atatgcgcca
     8521 aaacgcgcaa ttcaatctgt gaatagtcag agctgagtag cacactatcc tcccactctg
     8581 gcacaaaagc cttccgaatc aagcgccctt gttccaatcg ggcaggaata ttttgcaagt
     8641 ttggatccac actagacaaa cgcccggtct gggtcaaatc ctgcacatag cgagtatgaa
     8701 tctttccatc agccaaaatc cagtcctgca agccaattac ataagtagat tgaatcttag
     8761 caatttgacg gtaatccagg attttcttaa caatcggagc aataggagcg agacgctcca
     8821 aaacatccac tgctgtcgaa taacctgtct tggttttctt agtgtattct agaggaagtc
     8881 ccaatttctc aaagagaagc acgcccaact gcttaggcga gttgacatta aactcctcac
     8941 cggccagctc gtaaatctct tgagtcagtt tttcaatgac aagctcattt tcagcctgca
     9001 tctcaagcaa ggtctctttc ttgaccataa tcccagcaat ttccatcttg gcaaggacaa
     9061 aagccagagg ttgctccata tcataaagaa gctctaattg cccattttcg ctgagttttt
     9121 caagtaaaat aggctctgtt tctaccaaaa cagcaagttt acaagctaag tgttccaaga
     9181 atttctcacg ttcaggaatg gcctttttaa cacccttacc gtagaaagtt tcatcatcaa
     9241 ccaagtaagt ctgaccataa agactagcga tggtcgcaat ttcattgtcc tccacagtcg
     9301 aaaggaggta tttagccaaa cggatgtcaa aagcaggcgc ctgcaaatct acaccaaaac
     9361 gttgcaaaag aactttaacc ttcttaaagt cataaactct cagagatgtt ttttctaaga
     9421 aatccttgaa aatcgggtct tgcaacagct caagcttgtc tgtggcatag agctgatccc
     9481 cacaagacca ggcaaatcca accaaattat ctgtatggta attctcacca aaaagctcaa
     9541 agtggaagat agactcttca ctcagcatat cttgactgat ttggtcaaca atagtaaaat
     9601 ccaaaccctc agccacatca gctgacgaca catttaaagc ctgcttgagc tgtttgaagc
     9661 ccatctcatc gtaaaatttc ccaagatttt caacatctgg accactatag accaagtcct
     9721 ctaaaccaat cgcaatcggt gccttggtat caatggtcgc tagtgtttta gacaaaaagg
     9781 cctgttcctt gtcattgatg agattttcct tcatcttaga agtcttcatt ccatcaatat
     9841 tttcataaat cccctcaagc gaaccatgct ccagcaagag cttaataccc gtcttttcac
     9901 cgactttggt caccccaggg atattatccg acttatcacc catgagcgcc ttgagatcga
     9961 taaactgagc tggtgtgagg cccatttctt ccatgaggta atctggcgta aaggcctcaa
    10021 actcagccac acctttcttg gaaatttcaa ccaccgtatg ctcatccgtc agctgaatca
    10081 aatccttgtc cccactgaca atggtaatat caaaaccatc ctgctctgct agcttatcca
    10141 gcgtcccaat gatgtcatcc gcctcatact gagccagctc atagtgacga atccccatat
    10201 gatccagcaa ctcacgaatg aaaggaaatt gctcacgaaa ctcatcagga gtcttggccc
    10261 gaccaccctt atagtccgca tacatctctg tccggaaggt cgtctttccc gcatcaaaag
    10321 ccaccaaaat atgactcggc tcaacccgct ccaacaaatg actcaacatc aactgaaaac
    10381 cataaatcgc attggtatgc aaaccagccg cattcttaaa acggtccaac tgctgataca
    10441 gcgcaaaaaa cgcccgaaaa gctacagaag acccatcaat caataataat tttttcttat
    10501 ccatacaccc attataaagg aaagaatcaa aaaataccat tgggaagagc tagagcaagt
    10561 atttttcaaa ctttttccga ataaatagat agagccagag aatttagtaa acctagattt
    10621 aaaaatgtgc tataatatag tatattgaat ctataatagt acac
//
LOCUS       DAVKHZ010000071        10330 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000071 DAVKHZ010000000
VERSION     DAVKHZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.071"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(23..640)
                     /locus_tag="VWW99_001710"
     CDS             complement(23..640)
                     /locus_tag="VWW99_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9539518.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(630..1403)
                     /locus_tag="VWW99_001711"
     CDS             complement(630..1403)
                     /locus_tag="VWW99_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9539519.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(1414..2151)
                     /locus_tag="VWW99_001712"
     CDS             complement(1414..2151)
                     /locus_tag="VWW99_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9539520.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(2156..3286)
                     /locus_tag="VWW99_001713"
     CDS             complement(2156..3286)
                     /locus_tag="VWW99_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9539521.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(3370..3759)
                     /locus_tag="VWW99_001714"
     CDS             complement(3370..3759)
                     /locus_tag="VWW99_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9539522.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(3775..4158)
                     /locus_tag="VWW99_001715"
     CDS             complement(3775..4158)
                     /locus_tag="VWW99_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9539523.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(4159..4947)
                     /locus_tag="VWW99_001716"
     CDS             complement(4159..4947)
                     /locus_tag="VWW99_001716"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9539524.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(4940..5875)
                     /locus_tag="VWW99_001717"
     CDS             complement(4940..5875)
                     /locus_tag="VWW99_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9539525.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(6005..6181)
                     /locus_tag="VWW99_001718"
     CDS             complement(6005..6181)
                     /locus_tag="VWW99_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9539526.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(6322..7029)
                     /locus_tag="VWW99_001719"
     CDS             complement(6322..7029)
                     /locus_tag="VWW99_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9539527.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            7180..8208
                     /gene="queA"
                     /locus_tag="VWW99_001720"
     CDS             7180..8208
                     /gene="queA"
                     /locus_tag="VWW99_001720"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9539528.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(8877..10004)
                     /locus_tag="VWW99_001721"
     CDS             complement(8877..10004)
                     /locus_tag="VWW99_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9539529.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNASGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT         3043 a         2347 c         1841 g         3099 t
ORIGIN      
        1 caaattaaaa acatctttca tttcacttcc tacgactttt cagacacaga tacccaaaga
       61 agctttcata gagggcaaaa aagaggagga aggcatgaag aaagaaggtc tctggcaaaa
      121 tcataataac aggatccttg gctggatcaa aaagccaggt atcatctccc acaaagagaa
      181 tttgatggaa aagagtaaag aattggtcaa aaccaatcaa aactccccca agtccaatca
      241 tcacaggtaa gactactaga gtcaggagac tttttcgata aagagacaaa aagtcctttt
      301 tcacaatcct attgacaaag acatagaaac ttggcagtgt cactagagct actagctgaa
      361 ccaaatgaaa gagattcttg accactgcga aatggtgcag accagctgct gacgaacgaa
      421 aatcaggcat ctgtaagacc tgactaaaag gattggtcag ataattcatc aagatatgaa
      481 aattgtattg aatggtttct ggttttagat agactcgatt cgttaagttt agccactgaa
      541 tctccatagg atagaaaatc caagccagat aaatggtcag aaggattgag agggagagga
      601 gaaagagcat agagccccaa aagatcaatt tagttttcat caaaatccca ctccgcaagg
      661 ctagaaacca catgtgtcgg tgcgattggc aggccagcta cttcttctgc cttagtaaaa
      721 cctgtcgtca ccaagagcgt tggaatgcca ttgtcaatcc cagcccggat atcagtcaaa
      781 taattgtccc caaccatgat taactcttca cgttccaaac ctaagtgctc aaccgccttg
      841 tccataatga tggcatttgg ttttccgata taaaccggct tcactcgtgt cgctacttca
      901 agcagcgtaa tcagtgagcc agcacctggc aaaagaccgc gttccgtcgg gatgttgagg
      961 tcaggattgg ttccgataaa atgggcaccc ttttgaatag caagagttgc tgtggcaaat
     1021 ttttcatagt cgacttgcca atccagacca actaccacgt aggcagggtt ttccttgtct
     1081 tccacataac cagccgcctt gatggcttcc ttgagtcctg cttctccgac gacatagacg
     1141 gtcttttcaa gccccaaatc attcatatag tcgatggttg ccaaagtcgc tgtgtagaca
     1201 gtcgataggg gcgtatcgat attaaaattc tgagccaaca tctccttaac actctctgga
     1261 gtgcgggttg tattgttggt tacaaagaga tagggaatgt cccgcttttg caattcatga
     1321 acaaaagtct ctccagcagg gattcggtct ttccccttat aaatggttcc gtctaaatca
     1381 attaaatagc ctttatattt catctatttc tccctaagcc ttttttattt cttgccaagt
     1441 aatgattgct tgggcattga taaccccatc acttgtaatt tcatgcttgc tttccagtcc
     1501 agtccgttca acagccgatg taatcacccc acctggtcga acttccttga catacttgag
     1561 gttgattttc ttgggaatat agtgggtcaa aaaatccgct cccatgacct caaaaatcca
     1621 gtccaagtat ttactgttat tgacatgacc attcatatcc aagtcgtaaa aacgaacatg
     1681 gtaatccttg ctgatcggct cttccaagga ttcatacttc ggtccacgga taagtttttt
     1741 atcaaaatca gactggtaag gagccacaat ctcaggttca acaacatgga cttttcgact
     1801 gtcgcggtcc atgagaacaa aggtcgccat catgtggatg agctcctgcc ccgcttcatt
     1861 ataaatagta aagcgacggt agcaaaaaag tcgattgtag ctcaaggctt ccgtttcgat
     1921 ggtaatttct tccgcaaaac gaggcaaacg aaccacctca atatcatatt ctgcgataat
     1981 ccagaccaga ttatagtctt ccaaaatggc cttatcacta actcccagtt caatcgactg
     2041 catccctgaa acttgcagtg acagcaaaat cacatctgga agtttgatat gaccgttcat
     2101 atcagccata tcaaaaggaa ttttcatttt catttgataa gttaagccca tgatcctact
     2161 ccaaaataaa tcgttctgct acagtatctc ccaaaaagag acctctcttt gtcatgcgaa
     2221 cgtggtcacc ctcaatctgc atgaggcctt gttgaaccaa atctctgaca atttctccat
     2281 aaagtccagc aaaagactgt ccaaattttt cctcaaatcg cgccatggaa accccggatt
     2341 tcttgcggag tcccaagaac atttcttctt ccatttgctc cttttgactc aggtgatctt
     2401 ctgtaataca agcattgcct tcctcaaccg cactaagata atgacgaatg ggaccatgat
     2461 ttttataacg tactccattg acataaccag atgcccctgc accaatacca tagtattcag
     2521 cattgtccca gtacatgaga ttatgacgac tttcaaagcc gggtttggag aaattagaaa
     2581 tctcataatg ctcaaaaccc gctcgctcca gctctgcaat gatgtactca aacatctccg
     2641 cttctagttc ctccttaggc agaggcaatt tcccacgtcg catccggttc ataaagaccg
     2701 tatggttttc taaaatcaaa ctatacaaac tcatgtgggg aatatccagt ccaatggctt
     2761 tagccacatt ttcctttact tgctccatgg tctgaccagg cagagcataa atcaaatcaa
     2821 tggagatgtt gtcaaaacca gccagtttca ggcgatcgat attttcataa atatccttct
     2881 ccaaatgact gcgcccaatc tttttcaaca tcttatcatc aaaggtctgg acacctagcg
     2941 aaacacgatt gacagccgaa tttttcaaaa cagctatctt atccgcatcc aaatcgcctg
     3001 gattggcttc aatggtcaac tcttctaaga cagacaagtc caagttttta gtcaagccat
     3061 tcagtaaaac ctccagttgc ggagccgaca gggctgtcgg tgttccacca ccgatataaa
     3121 gggttgacaa cttttcaata tcataagaac gaaactcttc cagcagatgc tctaaatagc
     3181 tgtcgactgg ctgatttttg atgaagacct ttgaaaaatc acaataataa caaatctggg
     3241 tacaaaatgg gatgtgcaca taggctgacg ttggtttttt ctgcatagta attattatac
     3301 cacaaagact agattccaga taaaaatcac catccccaga tacatagtcc gttcggagat
     3361 ggtgatggtt tattcttctg ttatatcaat cacaatctct tctgagtcat caagagcttc
     3421 ggctttttct tgccattgtt ccttgagatt atttaattga ttttttgatg cttctgtcgc
     3481 ttgaaaagca taggatttag cttgagcaag tatactgtcc acagtgattt cacctgactc
     3541 aacctgttct tttgttttca gaacaaaatc tgtagcctgc tccttaactt ctgtcagttt
     3601 ttcacagact tgctccttgg catactccgg atcttctctc aaatcatcta gaaaatcttg
     3661 agcctgactg caaacttgtt tgcccttatc acttgttaaa aacaaggcaa gagctgcacc
     3721 tgaaacggtt cctaaaagga ttgaggataa tttacccata aggattctcc ttttttattt
     3781 tttgaaaaat ttacttgcaa gacgaagagc tgacagactt gcaccagtct tgagtgtttt
     3841 tgaaccagct gatgaagctt tcttgctcaa gacacgcgca tggtcattga ggtctgaaac
     3901 agatagagat aaatctgcaa cagcactgaa gagtggatca atcgtagcca ccttgacatt
     3961 gatatcatct gccaagacat tgaccttagc caacaactca ttggtgtgat gcaaggtcac
     4021 atccacatct gaagtcaagg ttttaatcgt cttttctgtt tcatcgatga cacgaccaag
     4081 cttttgtaca gtaatgatca gatagaccaa aaagacaatc aaagctaggg caacaagaat
     4141 atatgcaact tctaacattt agttttcctc ctctgtaata tagtaagggg ccttctttcg
     4201 attttgataa ataacgatca ttataccgag accgataagg acaactgaca gccattggga
     4261 cactcgaagt ccgaagaaca tgagactatc tgttcgcata ccttcgatga ccatacgacc
     4321 gaaaccatac caaatcaagt aaaaggccgt gatatgacct cgtctgagac tcttccattt
     4381 ccgtctaaaa atcagaatca aggcaaagcc aagcagattc catagagact cataaaggaa
     4441 agtcggttga cggtagctcc cctcaatata catctggtca cggataaagc caggtagata
     4501 atccagatta tccactgttg caccataagc ttcttggtta aagaaattac cccaacgccc
     4561 caaactttga gcaatcataa cgctaggcgc cgcaatatct agaaagtccc aagtattgat
     4621 gagtttacgg tcagcaaaga tatagagcac aagagcccca gttatcaaac caccgtaaat
     4681 ggccaaacca ccattccaaa tggcaaaaat ctctcctaaa ttctgactat agtaatcaaa
     4741 tcggaaaata acatagtaga gacgagctcc taaaatagcc aagggaaagg ctactaagat
     4801 aaaatctaaa atatcgtctg gtatgatctt ctttctaggt gcttctttca tggtcaaata
     4861 aaccgcaaga atcaagcctg tcacaataca taaggcatac caacgaatgg ctaggggtcc
     4921 tagttgaata gcaattggat caagcatttt gcacctcatt tcgagcgatg agacttgtca
     4981 gtcgttcgtc gaacaaacgg gtcgcatcaa agcccatttc cttggcacga taattcatgg
     5041 cagctgcctc aatcacaaca gagatattac gacctgtttt aactggaata cgaatacgag
     5101 gaatggctac gccagaaact tcaagttcct ctgcattgtt tccaagacga tcaaaggtct
     5161 tatgcgtatc gtaattttcc aaatagacag caagctgaac ctgtgaagaa tccttgacag
     5221 cactcgcacc gtagagactc ataacatcga taataccaac cccacgaatt tcaatcaagt
     5281 gtttcaaaat ttcagctggt tcaccccaga gagtaatctc atccttggca aagatatcga
     5341 cacggtcatc ggctaccaaa cggtgaccac gtttgacaag ctcaagacct gtctcgctct
     5401 taccaattcc actatctccc tgaatcaaga cgcccatccc ataaatatcc atcaagacac
     5461 cgtgcacact ggtacgttct gccaaacgag aatccagata gctagataac tctccagata
     5521 aacgactggt agctgcacgg ctggttaaaa tagcaatctt acattctcta gcagccttta
     5581 acatctcctc tggaaccacc aaaccacggg caacaatgac tgctggtgtc tcaggtagaa
     5641 acattttttt caaaacttca taacggctgt tggaaggcat gctgatcaga taagaccact
     5701 ccttcatccc caaaagttga atccgctctg gtgtatagta gtcaaaatag cctgtcattt
     5761 caagaccagg tcgcgtaata tccgctgtat tgatttcctt ttcaagcaat tctggttcac
     5821 catagacaat gtctagtcta agcttttcaa tcacttcttt tactaaaacc gacatcattt
     5881 cctccttcaa atgagttctt ctctctatta tatcaaattg taggaggaag tttcagattt
     5941 ttgcaggctt tggagtattt atttaaaaga aaaaggacta gatttctcta gcctttcatt
     6001 tcaattagaa ttttttacgt ttacgagcta cttctgattt acgtttacgt tttacagaag
     6061 gtttttcata gaattcacgt ttgcgtgttt cttgaagagt accagcttta gtaaccgcac
     6121 gtttgaaacg gcgaagtgcg tcgtcaagag attcattctt acgtactact gttttagaca
     6181 tttttttcac tcccttcaag ttcaatatct atttcattct acacgtaaaa ggaataattg
     6241 tcaagcaaaa atgcggttta attttgattt ttttcttcca tttatacaag agttgaaagc
     6301 gttcaataga acattgcttt tttatttttc gagtaagcta agcgcttcag catctgcgat
     6361 gatggttaca tcagggtgat tttggaggct acttgcaggt aggttctcag tcactgggcc
     6421 agatactgtt ccggcaatgg cttctgcttt cgactcaccg taagcaaaaa gaataataga
     6481 cttggcatcc aaaatgtttt taatccccat tgaaatagct tgggttggga cgtcttcaat
     6541 cttagcaaag aagcgtgcat tagcttcgat agtagactgg tcaagttcta ctagatgcgt
     6601 ttgactgtca aatggagtac caggctcatt aaagccgata tgcccattgc gaccgattcc
     6661 caagatttgg aggtcaactg gatggtcagc caaaatttgg ttgtagcgtt caacttcagc
     6721 ttcagcattg tccttaacac cacgaggcaa gaaactttct ttaaatggtt tttggttgaa
     6781 caagttttct tgcatgaagt aacgataaga ctgtggattg tccccatcaa ggcccacata
     6841 ctcatcaagg ttgacactgg ttagatttga aaaatcaagg tcactctcaa caatttcctt
     6901 gtaaaattca agtgggctac ttcctgtcgc aagtcctaga gtttgagctc cattagccaa
     6961 tttttccttc aaaatctcaa aagcaacttt tccaccttga acttggtttt caactttaat
     7021 aactttcatc ttttcatcct ccatttctgt ctatatttat tatatggtat agaccaattt
     7081 ttgtcaagtg aaaacgattt aatttctaaa aaaagagcgt ccaaacttga aacgtgttat
     7141 aatggatagg attagaactt agaaagaatg aacagatata tgaatacagc tgattttgat
     7201 ttccacttgc cagaggaatt gattgcccaa acgccccttg aaaaacgtga tgcctccaaa
     7261 ctcctcatcg tcaaccgtga gacaggagaa atgcaagata aacacttcca ctctattatt
     7321 gatatgctgg aacctggtga tgcccttgtc atgaacgaca cccgagttct ccctgcccgc
     7381 ctctatggtc aaaaagtgga gacaggaggt catgtggaac ttctcctcct taagaacact
     7441 agtggagacg agtgggaagt tctggctaaa cctgccaaac gcctcaaggt cggtactcgt
     7501 atcagctttg gtgatggccg cctcagcgct gtcgttacag aagaattgac ccacggggga
     7561 cgcattgtcc gctttgaata ccaaggaatt ttcctagaag tcttggaaag tctgggagaa
     7621 atgcctctgc caccttatat ccacgaaaaa ttagatgacc gtgaacgtta tcaaaccgtc
     7681 tacgccaagg aaagcggctc tgctgcagca ccgactgctg gtcttcactt caccaaagaa
     7741 ctgctggcag aaatccaagc taagggtgtt catctagtct atctgactct ccatgtcgga
     7801 ctcggaacct ttagacctgt ttctgtggat aatctggacg aacacgaaat gcactcagag
     7861 ttctatcaac tttctgagga agctgctgcc acccttcgct ctgtcaaaaa aaatggtggt
     7921 cgtgtcatcg ctgtcggaac cacttctatc cgcaccttgg aaactattgg ttccaagttt
     7981 gatgggcaaa tccaagcaga ttctggttgg accaatatct ttatcaaacc tgggtatgag
     8041 tggaaggtcg tggatgcctt ctcaaccaac ttccatctgc caaaatcaac tctggtcatg
     8101 ttggtttctg cctttgcagg ccgtgaatta gtcttagatg cctaccacca tgccatccaa
     8161 gaacactacc gcttcttcag ttttggtgac tccatgttta tttattgaga aagaatttct
     8221 ctaaatcttc taataccaat aaatcgctaa gatattattt caaagaacat ctacaattga
     8281 aactctagct agctgtagaa gacgcctagt acattgaaat taaaatgctt ccccctagct
     8341 tcgaaaatat tgccatagat tgcgttgact ctccaaattg attcatctat attttatttc
     8401 agcttcctat attttcttcg ctgtttgtaa atcaaaatgc aagacacatg agtagcacca
     8461 tatttgttac tcttatctgt cctctcaaga gactattatg agttatttca gaatcattca
     8521 ctactttgac cctgactctc cttagtctca aaatcaaaga cttatactct tcaaaaatct
     8581 cttcaaaccg cgtcaacgtc accttggatt atatatgtga ctgacttcgt cagttctatc
     8641 tacaacctca aagcagtact ttgagcaacc tgcgactagt tttctagttt tctagtttgc
     8701 tctttgattt tcattgagta ttaaacaaaa agtgaacaaa tctgaattct aatgtacaga
     8761 agactaggct tgttcacttt tttatagtcg ctataagatg accttatcta tagcttttta
     8821 tatataatta tatattcaga catactatta tcaattttgt ctcagggagg aatctgttaa
     8881 cgcacccatt caccattatc attgactcta tagccatcta tacttgtatt gaccgctaac
     8941 tcacccgatg catttacata ataccattta ccaccaactt ggaaccattg attaactttc
     9001 atagaaccgt tgctgttgag gtagtaccat gaaccattaa cttgtaccca acctgttgcc
     9061 atggaaccat cagtattata aaaataccac ataccatttt cttgtttcca gcctgttgtt
     9121 ggagcaactg ctttagctgg ttctactgct acatctgttc cttggttaga tgtaacagat
     9181 acaggatacg aaggaataga tgattgctca ggaacaacaa ctttttcagg ttctctcgtc
     9241 cctctcctta tacgtctttt taccatctct ttagtaattt gacgagaagt agtttcttca
     9301 attgttccat cacgttcatc tacagcatag attgtagtaa gagtaatttt accaatttct
     9361 cctacttctt gacctttatc aagagttggg tcatcgagat attctgtttc gattggaatt
     9421 tcttggacaa gaacttgggg cttggttcct tttttaacaa ctcttgtttg agagtctttt
     9481 ttttgactta aagtactctc agttacttgt ccactctttc catctacatt ataagttatc
     9541 gttgtaactg ttttcccatt ctttcctaga gtaatctctt gctcctgtcc tgcagaaagg
     9601 tcattgtctg cttcatattt agtagcaaat ggaacaagaa cttcttcaac cttgctttta
     9661 gctggaactt tgataactgt atccgtggct tcttttctat caacagtaac ctgttcggta
     9721 acataaccag tctctggatt aacatcgtag gtccttgtcg tagttacata gccatcctct
     9781 ccatcaattg taacaggatt ttcactacgg tcttttgttt catctttttc ataacgaatt
     9841 cgcgtacttg aaattttctt ggttactacc ttaggtttag tcgctacttt tacaataata
     9901 tccccattgt cagcgtcatc atactctatt ccctcttctt tatctctagt atcatctctg
     9961 acatattgaa tcccatcagc agcatgaaca aaacttgtat tcagattcct cctaaaaata
    10021 aagttagccc gattaccgca gaaccaaaaa tctttccgag tttacgtatt gcatagcgct
    10081 tattagtatt agattttgcc attacatcct acttctagta tagcatcttt tctatcaaac
    10141 gttaaacaat atactttata tataaaatag acttagaatg atatatttac tattgaacta
    10201 acactttaac tatatcgtaa tcaatctcat atataaagga ttgcagacat cttatctaaa
    10261 tacatgctaa tatatttaga tacaaacatt ccaacttgat aactttcact catctttcat
    10321 cattccttat
//
LOCUS       DAVKHZ010000072        10188 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000072 DAVKHZ010000000
VERSION     DAVKHZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.072"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(1..242)
                     /locus_tag="VWW99_001722"
                     /pseudo
     CDS             complement(1..242)
                     /locus_tag="VWW99_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            654..749
                     /locus_tag="VWW99_001723"
                     /pseudo
     CDS             654..749
                     /locus_tag="VWW99_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1002..1622)
                     /locus_tag="VWW99_001724"
     CDS             complement(1002..1622)
                     /locus_tag="VWW99_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9539530.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIVQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1622..2104)
                     /locus_tag="VWW99_001725"
     CDS             complement(1622..2104)
                     /locus_tag="VWW99_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9539531.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(2104..2946)
                     /locus_tag="VWW99_001726"
     CDS             complement(2104..2946)
                     /locus_tag="VWW99_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9539532.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(3041..3841)
                     /locus_tag="VWW99_001727"
     CDS             complement(3041..3841)
                     /locus_tag="VWW99_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9539533.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3985..5364
                     /gene="glmU"
                     /locus_tag="VWW99_001728"
     CDS             3985..5364
                     /gene="glmU"
                     /locus_tag="VWW99_001728"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9539534.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            5374..5919
                     /locus_tag="VWW99_001729"
     CDS             5374..5919
                     /locus_tag="VWW99_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9539535.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5931..6245
                     /locus_tag="VWW99_001730"
     CDS             5931..6245
                     /locus_tag="VWW99_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9539536.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            6262..6954
                     /locus_tag="VWW99_001731"
     CDS             6262..6954
                     /locus_tag="VWW99_001731"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9539537.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            7031..7522
                     /gene="rocS"
                     /locus_tag="VWW99_001732"
     CDS             7031..7522
                     /gene="rocS"
                     /locus_tag="VWW99_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9539538.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7620..8210
                     /locus_tag="VWW99_001733"
     CDS             7620..8210
                     /locus_tag="VWW99_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9539539.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            8219..9982
                     /locus_tag="VWW99_001734"
     CDS             8219..9982
                     /locus_tag="VWW99_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9539540.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3140 a         1771 c         2152 g         3125 t
ORIGIN      
        1 tgttccaatt gattaagtga tttaaatgtt tgctcatagc cataaaacat ttcggatttt
       61 aaaatgccaa agaaagattc catcctaccg ttgtcttggc tgtttccttt gcgtgacata
      121 gatgcttgaa ttcccttact ctctaggaag cgatgataag aatcgtgttg gtattgccag
      181 ccttggtcac tatggagaat cgtattctcg tagtgcttct ctttgaatgc ctgttccaac
      241 attaacgatc aatcaattta atcatgtacc taagattaga attgtttatc ccaaatttat
      301 ttgaaagctt ctctaagcta tatccttgtt ttctaagttc atagatctga actttatcat
      361 cataagttaa tttcataata aaaacacccc aaaagttaga ttttttctgt ctaacttttg
      421 gggtgtagtt catgtacacc tgatatgatg cgttttataa ttttaaagac tttttgacca
      481 gcctcatttt ttaacttgat actcagtgaa aagcaaagat taaactagga agctagctgt
      541 aggctgttca aagaacagct ttgaggttgc agataaaact tgtgaggtaa ccaacatata
      601 taatgtgaag ctgacgtggt ttgaatagat tttaggaggg tatgagtctg gaagttttaa
      661 tggataatgc aagattccat agaatgggta agctagagtt cttatgtgaa gagtttgggc
      721 ataaactttt accttttcct ccctactcat cttagtatag aaaagtgaat ctgaaatagt
      781 acataactgc ttctaaaaca ttcttataaa ttgatttaaa ttctcaaatc atattattca
      841 gttcttattt cattttgctc tacaatcctg ttgagaagac acgtgttcat atcaaaaagg
      901 tattggcaag ttgcaatacc tttttacaag gttcttttgt cttatttttg tttcaactga
      961 ctatatctcc tatggttcta gttcagaagg ctaggctata attatgattg ataagaagta
     1021 tcattccaag tattgagagt gaatgtttca aaatcatggg tttctataat ggtcaggctg
     1081 gcatttgcta gaccgccatc tttacgaaga agtggttctt tatagcctag gagagtacga
     1141 agactggcag taagattggc gccgtgtccg acaattagaa tacgctcagc tggactatct
     1201 tttaatgatt tgataaattg gatggtccgc tgagttgtac tatagaggga ttcggctccg
     1261 aacattcgag tgtcaaattg agcaagattt gaacgaaaag cctggatttg ttgcgggtaa
     1321 atagcttcca gggttgcaat tttcaaacct tctaacttcc caagttgcca ttcacggaga
     1381 ttaggaacga tttctaaaga acagggggta tggagttgac tttggacaat ctcagcagat
     1441 ttgaccgctc gaggtaaatc acttgaataa atctgatcaa aaggaatttc cttgagatac
     1501 tggccaagtc gttttagggt ttcaatggat tcaggaagaa gaggagaatc accgctagca
     1561 ccttgaaaac gaccttcttg gttccagagg gtacgaccgt ggcggacaaa gtagagtttc
     1621 attacttgat gtcctccaaa atatctacaa aatctgcctt tacaaagcta gccaagtctt
     1681 gtggcgcgac gataatgctg tgtccgactt cgcctgcaga gacaatcatt cgatccaaat
     1741 ctagagcaat tttatcgata aaaatgggat aattgtgttt ctgacgaatt ccgacaggat
     1801 tattggctcc atgaatgtaa ccagttgttt tttctaagtc cttttgtgga atcatgctca
     1861 cttttttatt gccagaaatt ttagctagtt tcttttcaga caagtgctga gtgataggga
     1921 caattccgat aatcggtccg gtcttgtctc ccaaaagcgc caaggttttg aaaatctgat
     1981 ctcgttcata accttgagga agctctcctt ctagggcatt gatttgaatc ccctgatgag
     2041 ggatagctgc tttagatagg atttgttcca ccaatgtttt tttgatttta actttttttg
     2101 ccattattta tatttatcct ccaattgact catccaaata ccaagccaga ttcccagcgc
     2161 aaagaagaag gcgatgatga cataaccgac aagtgaaagt cctgtgtatt ggatactttc
     2221 agcgtttcct gcatttggaa ttaagatcaa aagggtactt gataggacga taccgatgat
     2281 gaaatgatag acgcgtgagt ggtagttgtt taaggcataa tccatcaatt ttgaaaaaac
     2341 gatgagagtt gcacctgcac caattccaat cggaaagaag gttcccaaga gatcaaaagt
     2401 tttaaaacca gtcaacatag gagcatagag tcccaaaatc aaaagtaaat ttgatgggct
     2461 gaggccagga accaagacgc caagggccaa tagtgcgcct gctaggacga agttaagaaa
     2521 gctggcgctt aaggttccaa cgacaaaatt taaggcatag agtcctaatc cagaaatgat
     2581 aaaggttgtc cataaccaag ctaaatcaat cttgtctcgg tcagattctc gagttgattc
     2641 tttgaggagg ctaggaactg taccgataat agctcccgca aagctccata atacaaagac
     2701 ctgataattt tcaagcaggt attcaatcgg gtaggaaaat aagccgattc ctagaagcat
     2761 accgatggca actggaataa agtacaaaac attttcttta aagtctttaa agggatgggc
     2821 cagaaagcca atcattcgtt catagattcc taagattgct gctagaaccc ctccggaaat
     2881 tcccggtagg ataaatccaa gagcaattac aatcccttta ataacgcgtg ctaaccatga
     2941 gagcatatat ttcctccaac tatttctatt tcaaaaaatc cttactatat tgtatcacaa
     3001 tcagacacaa aaagaaaaag caaatgataa acaaatgctt ttaaggtttt aaaaaaagtt
     3061 ttcgaaaggt ttcttcttta ttttttaagg gagagataac gttgatatct aaatcgtggt
     3121 caaagccggc aatttttcct ttagatgtgt attggtgaat atcataatct aaatcagttt
     3181 taggactgct ctccaaaaat cctgagtctg agccgtagga cggaatccaa acagaggtaa
     3241 acttgcctgt atcaatactg tgttcttcca tgaagtagac accaacgtag atgccgatgt
     3301 ttttagcacc tagtgatgct agttttgctc gaaagttttc gacaccttcg ttcatattag
     3361 acatggtttt gtcttccacg tcaagccaat agtaactagg gctgtaagga gaggcagcat
     3421 tgtagaaaac ttcggcagcc ttttccattt cttggacact ttttccagct acataagcgt
     3481 agacagcaac tgggacattc cgcttttgaa gttcagtgat atgactctta taggccttgt
     3541 ctattccatt gataaatgaa gcatcatttt cttttgtcgt ttgagcacca ctgtgaacac
     3601 gaacaatagc acctgaaata ttttgtgaga gggcatcgta gttgatttct tcaggacgct
     3661 gccagccaga gaggtcaata atcggtttgt ctaagtgttt caaagcctgt gcttcaatct
     3721 gtgctatatt ggattttgtt ttaaacgatt ggttgtcatt aagtgggcga ttgatgatta
     3781 aaatgaacat cataatcccc aaaaaactaa ataaaataag tggatgaatt tgttttctca
     3841 tatcttataa ttctacccta aaaatcaaaa aaaatcaaaa aaatgggtta aggaagagac
     3901 tttagagcat tttttcattc aagagtgcgg aatgatttga aatatggtat aataaaaggg
     3961 aatttctaca gaaaagagaa gattatgtca aattttgcca ttattttagc agcgggtaaa
     4021 gggactcgca tgaaatctga tttgccaaaa gttttgcaca aggttgcggg tatttctatg
     4081 ttggaacatg ttttccgtag tgtgggagct atccaacctg aaaagacagt aacagttgta
     4141 ggacacaagg cagaattggt tgaggaggtc ttggctggac agacagaatt tgtgactcaa
     4201 tctgaacagt tgggaactgg tcatgcagtt atgatgacag aacctatctt agaaggtttg
     4261 tcaggacaca ccttggtcat tgcaggagat actcctttaa tcactggtga aagcttgaaa
     4321 aacttgattg atttccatat caatcataaa aatgtggcca ctatcttgac tgctgaaacg
     4381 gataatcctt ttggctatgg acgaattgtt cgtaatgaca atgctgaggt tcttcgtatt
     4441 gttgagcaga aggatgctac agattttgaa aagcaaatca aggaaatcaa cactggaaca
     4501 tacgtctttg acaacgagcg tttgtttgag gctttgaaaa atatcaatac caataacgct
     4561 caaggcgaat actatattac agacgtcatt ggtattttcc gtgaaactgg tgaaaaagtt
     4621 ggcgcttata ctttgaaaga ttttgatgaa agtcttgggg taaatgaccg tgtggcgctt
     4681 gcgacagctg agtcagttat gcgtcgtcgc atcaatcata aacacatggt caatggtgtt
     4741 agctttgtca atccagaagc aacttatatc gatattgatg ttgagattgc tccggaagtt
     4801 caaatcgaag ccaatgttac cttgaaaggg caaacgaaaa ttggtgctga gactgttttg
     4861 acaaacggta cttatgtagt ggacagcact atcggagcag gagcggtcat taccaattct
     4921 atgattgagg aaagtagtgt tgcagacggt gtgacagtcg gtccttatgc tcacattcgt
     4981 ccaaattcaa gtctgggtgc ccaagttcat attggtaact ttgttgaggt gaaaggatct
     5041 tcaatcggtg agaataccaa ggctggtcat ttgacttata tcggaaactg tgaagtggga
     5101 agcaacgtta atttcggtgc tggaactatt acagtcaact atgacggcaa aaacaaatac
     5161 aagacagtca ttggagacaa tgtctttgtt ggttcaaatt caaccattat tgcaccagta
     5221 gaacttggtg acaattccct cgttggtgct ggttcaacta ttactaaaga cgtgccagca
     5281 gatgctattg ctattggtcg cggtcgtcag atcaataaag acgaatatgc aacacgtctt
     5341 cctcatcatc ctaagaacca gtaggagcct atcatggagt ttgaagaaaa aacgcttagc
     5401 cgaaaagaaa tctatcaagg accaatattt aaactggtcc aagatcaggt tgaattacca
     5461 gaaggcaagg gaactgcccg acgggatttg attttccaca atggggctgt ctgtgtttta
     5521 gcagtaacgg atgaacaaaa acttatcttg gtcaagcagt accgcaaagc tatcgaggct
     5581 gtctcttacg aaattccagc cggaaaattg gaagtaggag aaaacacagc ccctgtggca
     5641 gctgcccttc gtgaattaga ggaagaaaca gcctatacag ggaaattaga actcttgtac
     5701 gatttttatt cagctattgg cttttgtaat gagaagttaa aactatattt agcaagcgat
     5761 ttgacaaaag tggaaaatcc gcgtccgcag gatgaggatg aaaccttgga agtccttgaa
     5821 gtgagcttag aagaagcgaa agaattaatc caatcaggtc atatctgtga tgccaagaca
     5881 attatggctg ttcagtattg ggagttgcag aaaaaataga ggaggtcagt atgggtaaat
     5941 ctttattaac ggatgaaatg attgaaagag ctaatagagg cgaaaaaatt tcaggtcctc
     6001 ctttgctaga tgataatgag gaaactaaga ttttaccaac ctcttcttcc cgttttggtt
     6061 atgccaatcc taaggatcat ggttttagcc aggaaacctt gaagattcag gtcgaaccat
     6121 ctattcataa aagccgtcgc attgaaaata ccaagagaaa tgtcttcaat tctaagttga
     6181 ataaaatctt attcgcggtc atctttctct tgattttgct tgttttagca atgaaacttt
     6241 tgtaatagaa aaggaattga aatgaaaata ggaattattg ctgctatgcc agaagaactg
     6301 gcttatctgg tccagcattt agacaatgcc caggagcaag ttgttttggg gaatacctat
     6361 catacaggaa ccattgcttc tcatgaagtc gttcttgtag aaagtggaat tggtaaggtc
     6421 atgtctgcta tgagtgtggc gattttggct gatcatttcc aggtggatgc ccttattaat
     6481 acgggttcag ctggggcagt agcagaaggt atcgctgttg gggatgtcgt gattgctgac
     6541 aaattagcct atcatgacgt ggatgtcaca gcttttggct atgcttatgg acaaatggcg
     6601 caacaaccgc tttatttcga atcagacaaa acctttgttg ctcaaatcca agagagttta
     6661 tctcaattgg accaaaactg gcatcttggt ttgattgcta caggagatag ttttgttgca
     6721 ggaaatgaca agatagaagc gattaagtcc catttcccag aagttttagc cgtggagatg
     6781 gagggggcag ctattgctca agcagcgcat gccctcaatc tcccagtctt agtcatcaga
     6841 gctatgagtg acaatgccaa ccatgaagca aacatctttt ttgatgagtt tattatcgaa
     6901 gctggacgtc gctctgccca agtcttgttg acctttttga aggctttaga ttaagcggaa
     6961 atttgacagt ttttctagct tatgataaga tttaagtaaa gaaaagctag aaaacgtttc
     7021 agaggatatt atgagtattg aaatgaccgt cagtgagatt gcagaggtct taggattatc
     7081 tcgccaagca atcaataacc gtgtcaaaga attaccagaa gaagacacag ataaaaatga
     7141 caaaggggta acagttgtta ccagaagtgg cttgattaag ctagaagaaa tctataaaaa
     7201 aacgattttt gaagatgagc ctgtcagtga agatgtcaaa caacgtgaac tgatggagat
     7261 tctagtggat gagaagaatg cagaaattct tcgtctttat gaacaattaa aagccaagga
     7321 tcgtcagtta tcagaaaaag acgagcagat gcgtatcaaa gaccgtcaga ttgctgagaa
     7381 agacaaacaa ttagatcagc aacaacaatt gacccttcaa gccatgaagg atcaagaaaa
     7441 tcttaagcta gagctggacc aagcaaaaga agaagtccaa tccactaaga aaggcttttt
     7501 tgctcgttta tttggaggat aatcaaggag ctgtttaggt taaatgctaa cctgatggct
     7561 tcttttgttt gtgaatagta tagttgatta gaacagagac agtagaggag aggtacagaa
     7621 tggaacgatt agaagattac attgcttttg atttagaatt taatcagcac gaaggacaaa
     7681 cacacttgat tcaagtatcg gcagtgcgtt ttagagatgg tcgggaaatc gatgcctatg
     7741 attcctatgt tcataccagc gtccctctaa aaagctttat caatggtttg accgggatta
     7801 cagctgagac cttaaaagat gctcctccag ttgagaaagt cttagaagaa ttccaagagt
     7861 ttgtgggttc tttgctgatg ataggctaca atgctgctaa aagtgattta cctattttag
     7921 cagagcatgg tttagactat agccaacagt acaaggtgga tttgtatgat gaagcttttg
     7981 aacgtcggag ttcagattta catggtattg ccaatctcaa attgcaaact gttgcctcat
     8041 ttcttggatt tcaaggtcag tctcataata gtttagagga tgctcggatg acggcgcgtg
     8101 tttacgaatc cttcttagag tcggatcaag ctagggaatt gttagagaca gaaagtagct
     8161 tatcaaacaa tccttttggt ggcttggact tgtctcaatt atttgactag gagtgcctat
     8221 gaaacctgaa aaacctaaaa aattgggtct taaacagatt tacctcaatc ttgataaaat
     8281 cctctttcta tttttcttga tttttatgat agtggagtat ttgtgggtac cattcaattc
     8341 atggttggct agcttcttat tagatcagac aggttatctt tttatttcct ataacaatgt
     8401 cttagccatc cttactcatt ctccattgat tagtctagct tttttagcct tgattgtaat
     8461 taaccttctg gttgcttatt ttcaaatagg actcctcttt attggtgctc gtcatctcct
     8521 ctataatgaa aagcgaaggc taattgagta tatccgcaag gttttccatg aaagttttgg
     8581 atttatgaaa aagctaactt gcgctaaagc cttgtttatc tttttctata tagctatgct
     8641 ttttcctttt atacggaaaa tgctgaagat ttattatctt aataaaatcg tcattccaga
     8701 gtttatccaa gcttatttag aagataaata ttggatgtgg tggctgagca tcctacttct
     8761 atctcttatc tttctctatg tctctgtcag attgatgttt gcccttccga agattgtata
     8821 tgatcagctg actgttcgag aagcagtgat gtttagcttg gagaaaacca agaagagagt
     8881 tactttttat gcttggaatt tattttacat cttgctcaaa gcacatttat tattttatct
     8941 tcctttgatt cctttattat tggctcaaac tttggtagat gatatcactc agagagagtc
     9001 tctgattctt ggtattttga attttgtcgt gattaaaaat ctctattata tggctctgac
     9061 ttattttttg gttaaatttg tttcattttt gacaggaaag gagctggata tgcttcctag
     9121 gagagaaaaa gatcacatcg tgcgatgggg tgtcatgact tgtgccagtc ttttctttgc
     9181 cttagaaggt tatatttatc tggaggctcc catggttcat ctacctcaac ttatttctca
     9241 ccgaggggtt tccaatgcaa atgggattca aaatacagta gagtccttgg aaactacagc
     9301 acaactcaaa ccagacttgg tggagacgga cgtgcaggaa acaaaagatg ggcagtttgt
     9361 catgatgcat gatgctaact tgaaaaatct agcaggtatc aataaaagtc ctcaagactt
     9421 aaacttggag gagcttaaag ggattgatat ttttgaaaat ggctaccaga ctaaaatttc
     9481 aagctttgaa gattatctca gtcgagccaa cgaacttggt caaaaattac taattgaaat
     9541 taaaaccagt aaaaaagata gtccagacat gatgaaccgc tttttagccc gttatgctgc
     9601 aaagctcaag atttatggac atcaaatcca gtctttagac taccatgttg tcgaaaaagt
     9661 aagacagtat gatgccgaac tgccagttta tttcatcatg ccctacaatt ctgtctttcc
     9721 taaaacaaga gcgacaggat atactatgga gtactcaacc ttggatgaat attttgtaag
     9781 caaactatgg acaacggatc agaaacttta tgtctggact gtcaatgatt cagaagctat
     9841 cagcaaatct cttcacttag gagtagatgg agtgattaca gatgatttgg aaaaagttca
     9901 gcgggaaata gaagtagccc aagaagatcc agaatatacg gatttgctct tgaaaaaagc
     9961 attggaattc tttgaatttt agtaataaaa aagaggaacg gttgttcctc ccatgatagg
    10021 taagtattct tgtgaatgct tgcttttttg tggttgcaat ccgtttacga gaattgtttg
    10081 tattgttctt gtttcaattt actttatagt gctttgaagt tggaatgcta cactataact
    10141 tctaaaatat agtaaaatga accaaaaata gtacacaatg tggtataa
//
LOCUS       DAVKHZ010000073        10182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000073 DAVKHZ010000000
VERSION     DAVKHZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.073"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            20..1891
                     /locus_tag="VWW99_001735"
     CDS             20..1891
                     /locus_tag="VWW99_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEU9539541.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGEPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLGVLKVT
                     QHNSRKTWYYVPLQDFTVNSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHVREMD"
     gene            2056..5310
                     /locus_tag="VWW99_001736"
     CDS             2056..5310
                     /locus_tag="VWW99_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9539542.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDQAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGNIQKLVDSYFENN"
     gene            complement(5696..6682)
                     /locus_tag="VWW99_001737"
     CDS             complement(5696..6682)
                     /locus_tag="VWW99_001737"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9539543.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            6876..9344
                     /gene="gyrA"
                     /locus_tag="VWW99_001738"
     CDS             6876..9344
                     /gene="gyrA"
                     /locus_tag="VWW99_001738"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9539544.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9344..10087
                     /gene="srtA"
                     /locus_tag="VWW99_001739"
     CDS             9344..10087
                     /gene="srtA"
                     /locus_tag="VWW99_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9539545.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3355 a         1699 c         2161 g         2967 t
ORIGIN      
        1 aatataagag aggttgctga tgacaataga tattagtgaa gaaagtttgg ctaaagaatc
       61 ggctgactta ttaaaaattc ttttaaaaga tcgaacgaca aagaaatcaa ttgtttgggc
      121 tacgcattct tatgaattgt taggaaaggg atttgctcca agtgatcgca tcaatccaag
      181 taaggttact ggaaactttg ccaacttgat tcagcccaga tctgaaaaat ccaaatatga
      241 acaaaaagat cggacaaaaa taagggcaga agtgtttaca ccaacgtggt tggtcgcaaa
      301 gcaaaatgga tatgtagaat cgaaattagg ctcgttaagt cttgaggagt atgtggactt
      361 acgatggtta gaggtaactt gtggtgaagc accttatatg gtaacgagat atgataccgt
      421 aactggtgag gaaattccat tatctgagcg agttggtttt gttgatagaa aactgcaacg
      481 gattagtcgt gaggtttcag atgaagtcac attttataaa cttgtaaaag atgcttatca
      541 tgcttcgtat ggttatgagt atcaaggaga ttctctcctt ttggcgcgtg agaatctttt
      601 ggcaactttt gaggactatt atctagctaa gactggaaat caaccaacat tagagcaaaa
      661 gaaagaaatt gcgactatta tctcttacaa tgtctttcag atggatggat tgaaaaaaag
      721 tagtccttat tcagctaaac aaaagcaatc tcaacaattg agtttgtttg cggatgagct
      781 agaagtccaa gaagtagaag aatctaaaac ccatatcaaa gactggaaaa agaatagaat
      841 gataggtttt gagcgcctat ctagcgagga aagtgaaatg aaatttgatg tcgtgatagg
      901 aaatcctccg tatcaggaaa caggagaagc cagagatgag ccgatatatc actattttat
      961 tgatgaagct tataaaatcg cagataaatc tatactaatc accccagcaa ggtttttatt
     1021 taaagctggt caaactccta aaaattggat ggaaaagatg cttgaagaca agcatttaaa
     1081 agttcaattc tatgaactta aaagcggtaa agtatttaca ggaacggata ttaagggtgg
     1141 agtagcaata acttatcgtg atgttgataa agatttggga ccaatcggag tatttactat
     1201 ttttgaaact ttgaatggta ttgtaaaaaa aatcgaaaaa catccagaat ttgaaacttt
     1261 ttctgattta gtttatcctc aaggtattta tagattttca aataaacttt tttctgatta
     1321 tccaaatgct gaaaagctgc aaggaaaagg gacaaaaaat aaaattgttt cgaaatcact
     1381 aactcagtta gattttgttt tcaaaaacaa cgaagaaaaa gaccatgttg ctctacttgg
     1441 aagaattggc tccgaacgtg tttatcgata tattaataaa aaatatttag atttaccgga
     1501 aacattcgaa aattataatg tttttgtacc agaagctaat ggaagtggtg ccttaggtga
     1561 agtcttatca acacccctaa tcggggaacc cctaatcggg catacagata cttttttatc
     1621 tattggtaat tttaaaacaa aatttgaagc cgatgcttgt attaaattta ttaaaactaa
     1681 attcgctaga gtattattag gtgttttgaa agttactcag cataattcac gcaaaacttg
     1741 gtattacgtc cccctccaag actttacggt caattcggac attgattgga cacaatcagt
     1801 gactgatatt gaccgccagc ttgatcaaaa atatgacctt tcccctgaag aaattgcctt
     1861 tattgagaat catgtaaggg agatggatta gaaaagtatt tttatttgac aaatagtgct
     1921 caatgatcta aaatgactat ataggattag gtcaggaagc atacgatgcc ctgacccttt
     1981 ttgtacttat gagatgagaa agtcatttgt tagataaatt gacttcgtta gcaaacgttc
     2041 aaaaaaggaa aacttatgcc agtagaaatt aaaaccacta aagaaattca tcctaaaatc
     2101 tatgcctaca ccacaccgac agtaaccagt aatgaaggct ggattaagat tgggtataca
     2161 gaacgtgatg tcacacaacg tatcaaggag caaacgcata cagctcatat agctacagat
     2221 gtcttatgga ctggtgatgc agcttataca gaagagcctg ataaggggaa aactttcaag
     2281 gaccatgatt tccaccattt cctttctttc catgatgtag aacgtcgtcc caagacggaa
     2341 tggttctatt ttaatggaac tcctgaaaaa tcaaaaaatc tttttgataa gtttgttcag
     2401 catgatttgt ctggttatca gcctggaaaa ggacaggact atactctgcg acaagagcaa
     2461 gaagaagcag ttgctaagac attagcttat ttccaagaac atgctggagg caagtttctc
     2521 tggaatgcca agccacgctt tggtaaaacc ttgtctacct atgacctagc tcgacggatg
     2581 gaagctgtca atgtcctaat tgtaacaaac cgccctgcca ttgctaactc atggtatgat
     2641 gattttgaaa cattcatagc aggtcaaacg acttacaagt ttgtttctga atcagatagc
     2701 cttaagagtc gtccaatctt gtcacgacaa gaatttcttg gtattttagc tgacgatgta
     2761 agacaacttg cttttatcag tctccaagac ttgaaaggat ctgtttattt aggtggagag
     2821 cacgataaac tcaaatgggt aactgatctg cattgggact tgttggttat tgacgaggct
     2881 catgaaggag ttgatacctt caagactgac caagccttta ataagattcg acgaaatttt
     2941 actctgcatt tgtcaggtac atcatttaaa gcattggcta aaggagattt tacagaggaa
     3001 caaatctaca actggtctta tgctgatgag caggctgcta agtattcgtg gtctcttgag
     3061 caagaagagg aaaatcctta tgaaagcttg cctcagttga atctctttac ctatcaaatg
     3121 tctcagatga ttggcgaaaa gttagaaaaa ggcgctcaga tcgatggtga aaatattgac
     3181 tatgtttttg acttaagtga atttttcgct acagatgata aagggaaatt tattcatgag
     3241 catgatgtca gaaattggtt agatactcta tcaagcaatg aaaaatatcc attttcaacc
     3301 aaagaactcc gtaatgaact caagcatact ttttggcttt tagaacgtgt cgcttcggcc
     3361 aaagcattaa aagccctact agaagaacac ccaatctatg aaaactatga gatcgttcta
     3421 gctgctggtg acggacgtat gtccgaagaa gacgataaag tcaaactcaa atccttggac
     3481 ttggttagaa aagcgatagc agagaatgac aaaaccatta ccctatccgt tggtcagctg
     3541 acgacaggtg tcactatccc tgaatggaca ggtgtattga tgttatcaaa tttgaaatca
     3601 ccagctcttt atatgcaggc cgccttccgt gctcaaaatc cttactcatg gagcgataac
     3661 aaaggaaatc actttcgcaa agaaagagcc tatgtatttg actttgcgcc ggaaagaacc
     3721 ttgattctct ttgatgagtt tgccaacaac ttatcgcttg taactgcagc tggtagagga
     3781 acttcagcta cacgcgaaga aaatattaga gaattattaa acttctttcc aattattgcc
     3841 gaagaccgtg ctggtaagat ggttgaaatt gatgcaaagg cagttctaac cactcctcgc
     3901 cagataaaag ctagagaagt tcttaaacga ggttttatgt ccaatctctt atttgataat
     3961 attagtggta ttttccaagc aagtcaaaca gttttagata ttttaaatga gctgccagtt
     4021 gaaaaggaag ggaaggtaca agatagttct gatttattag atttttcaga tgttacagtc
     4081 gatgatgagg gaaatgcagt agtagaccat gaaattgtag ttaatcagca aatgcgactt
     4141 tttggtgaaa aagtttatgg acttggtgaa tctgttgctg agttagtcac aaaagatgag
     4201 gaacgaactc aaaaacagct ggtcaatgac ttgagtaaga ccgtttcttc agtgattgta
     4261 gaggaattga aagcagatta ttctctaaaa acaagggaaa ctgagcaaat taagaaacaa
     4321 attacagcaa cacttgagaa tgaaattcga aaaaatgata tcgaaagaaa aatttctgaa
     4381 gctcatatca agcaagagtt gcaacagcag ctcaaagaag caaatgataa agcgcaaaaa
     4441 gataagattc aagaagattt ggaaaaacgt ttagaagaaa ataaactcat tcataaagaa
     4501 aaactagaac aaacactcaa aaaagaagtg gaaaaaatgc ctgagaaatt tatcgaacag
     4561 gttgagataa aacgtgtgga acagttgaaa caatcagctc aagatgaaat tcgtgaccat
     4621 ttacgagggt ttgcaagaac aattccaagt tttattatgg cttacggtga tcaaactcta
     4681 acacttgata attttgatgc ctttgttcct gaacatgttt tttatgaagt aacagggatt
     4741 acgattgatc agtttagata tttgcgagat ggtgggcagg attttgcagg gcatctcttt
     4801 gataaagcaa catttgacca agctattcaa gaatttcttc gcaagaaaaa ggagttggcg
     4861 gattatttta aagatcaaaa agaagacatt tttgactata ttccaccgca gaagaccaac
     4921 caaattttca ctcctaaacg agtggtgaaa aggatggtag atgatttgga aaaggaaaat
     4981 ccagggattt ttgatgatcc atctaagact tttattgatt tatatatgaa gtcaggcctc
     5041 tatattgcag aacttgtgaa gcggttatat aatagcaatg gcttgaaaga ggcctttcca
     5101 aatcctgaag aacgcttaaa acatattttg gaaaagcaag tttatggatt tgctccgtct
     5161 gagattatct ataacatttc cactaatttt atatttggta atctttctaa agatatcagt
     5221 aggaagaatt ttgttttagc agataccatt ccagcggcta aagaagggaa cattcaaaag
     5281 ttggttgatt cctattttga aaataattaa aaagaaggcc gagtcaaaat tctttgaaat
     5341 cagaaaaaac gcataatatt gagtgctttt gtactgcccc ccaaaagtta gacagaaaaa
     5401 atctaacttt tggggggcag ttcagacaat ccttggtatt atgcgtttta ttgtgggaag
     5461 atgtataatg gattgaaata agatatgaac aaatcgatta ggaaaggaaa attgatttat
     5521 agaaatgttt tagtagtcga tgcgtactgt tatagattca acgaactata aaatctgaag
     5581 aaagcaattt taaccagatc ttagaagcag ttgtgtacta ctctaacttc aatagactat
     5641 atcatataga taaaaaacaa ctccctgatg attcaaggag ttgtctatag ttaaattagt
     5701 ttttagaagc ttcttggaat tctggatttt tccatgcttc gtcaataata gcttgcaatt
     5761 ctttagcaga tgcttgcatt ttttgagttt ctgcatcgtt caatggaatg tttactggac
     5821 gaacgatacc atgtgcacca acaacagctg gttgaccgat aaagacattc tcaactccgt
     5881 attgaccttc ttggaatact gaaagtggaa gtactgcgtt ttcatcgtca aggattgctt
     5941 tagtgatacg agcaagggct actgcgatac cgtagtatgt tgcacctttt ttgttgatga
     6001 ttgtgtaggc tgcatcacga acaccttcga acaattcaat caattcagct tcttgaacat
     6061 tttgagtgtc tttaaggaat tcttcaaggt ttacaccagc gatgttagcg tgtgaccaaa
     6121 cagcgaactc agagtcaccg tgttcaccca tgatgtaggc gtgcactgaa cgagcatcca
     6181 catccaattt ttcagcaagt gcttgacgga aacgagctga gtcaagtgaa gtacctgaac
     6241 cgataacgcg ttctttaggg aaaccagaga atttccaagt tgagtaagtc aaaacgtcaa
     6301 ctggattagc agcaacaagg aagatacctt tgaaaccaga ttcaacaact tgagttacga
     6361 ttgatttgtt gatagcaagg tttttaccta caaggtcaag acgagtttca cctggttttt
     6421 gaggtgcacc tgcagtgatc acaacaaggt cagcgtctgc acagtcagag tattgagctg
     6481 catagatttt tttaggtgaa gtgaaggcaa gggcgtgact aaggtcaagc gcatcaccaa
     6541 cagctttttc atgcaattgt ggaatttcga taattccaag ctcttgtgca attccttggt
     6601 taacaagtgc aaaagcgtaa gatgaaccta cagcaccatc accgacaagg ataacttttt
     6661 tgtgttgttt agttgaagtc attgttttaa acatctcctt aattttatta ggggattttc
     6721 cctagacaac ttcattctat cacttttaaa aaactttgtc acgaatatgc cttatagttc
     6781 tcgatgtaaa cgttttagtg gtttagaggc tgaaatagat gggaatttat ggtataatgt
     6841 tgttacttac taattgtgaa atgaggcatt tattaatgca ggataaaaat ttagtgaatg
     6901 tcaatctgac aaaggagatg aaggcaagtt ttatcgacta cgccatgagt gttatcgtag
     6961 cgcgagctct tcctgatgtt cgagatggct taaaacctgt tcaccgtcgc attctctacg
     7021 gaatgaatga attgggtgtg accccagaca aaccccataa aaaatctgct cgtattacag
     7081 gggatgtcat gggtaaatat cacccacacg gggattcctc tatttatgaa gccatggtcc
     7141 gtatggctca atggtggagc taccgttaca tgcttgtaga tggtcatggg aattttggtt
     7201 ccatggatgg agatagtgct gccgctcaac gttataccga ggcacgtatg agcaagattg
     7261 ctctggaaat gcttcgtgat atcaacaaaa atacagttga tttcgttgat aactatgatg
     7321 ccaatgaacg ggaacccttg gtcttgccag cgcgttttcc aaaccttttg gttaatggag
     7381 caactggtat cgcggttggg atggcaacca atattccacc tcataatctg ggtgaaacca
     7441 ttgatgcagt gaagttggtc atggataatc ctgaagtgac taccaaggac ttgatggaag
     7501 tcttgcctgg accagatttt ccaactggtg ctcttgtcat ggggaaatca ggtatccata
     7561 aggcttatga aacaggtaaa ggttcgattg tcctacgttc tcgtacagag attgaaacga
     7621 ctaagactgg tcgtgagcgc atcgttgtaa cagaatttcc ttacatggtc aataaaacca
     7681 aggtgcatga gcatattgtt cgcttggttc aggaaaaacg cattgagggt atcacagcag
     7741 tacgtgatga gtcaaaccgt gaaggtgttc gatttgttat tgaagtcaag cgcgacgcct
     7801 cagccaatgt tattctcaat aacctcttca aaatgaccca aatgcaaacc aattttggtt
     7861 tcaatatgct cgctatccaa aatggtatac cgaaaatttt gtctcttcgt cagattttgg
     7921 atgcttatat cgagcaccaa aaagaagtgg ttgttcgtcg tacacgtttt gataaggaaa
     7981 aagcggaagc gcgcgctcat atcttagaag gtctcttgat tgcgctagac catatcgacg
     8041 aagtgattcg tatcatccgt gctagtgaaa cggatgcgga agctcaagct gagttgatga
     8101 gcaagtttaa gctttctgaa cgtcaaagtc aagctatcct tgatatgcgt cttcgtcgtt
     8161 tgacaggttt ggaacgcgat aagattcaat ctgagtatga tgacctcttg gctctgattg
     8221 cggatttagc agatattctt gctaagcctg aacgtgtttc tcaaattatc aaagacgaat
     8281 tggatgaagt taaacgtaaa ttttctgata aacgccgtac agagttgatg gttggacaga
     8341 ttttgagtct cgaggatgag gacttgattg aagaatcgga tgtcttgatt accctttcta
     8401 acagaggcta cattaagcgt ttggatcagg acgagttcac tgctcaaaaa cgtgggggtc
     8461 gtggtgtcca aggaacggga gtgaaagatg atgactttgt tcgtgagtta gtgtcaacta
     8521 gcacacatga tcatctgctc ttcttcacaa acaagggacg tgtctatcgt cttaaaggtt
     8581 atgaaattcc tgagtatggt cggactgcca aagggctacc agtagtcaat ctcttgaaat
     8641 tggatgaaga cgaaagtatt cagacggtta tcaatgttga gtctgatcgc agtgatgatg
     8701 cttatctctt ctttacaacc cgtcacggta ttgtgaagag aactagtgtt aaggagtttg
     8761 ccaatattcg tcaaaatggt ctcaaagcgc tgaatttaaa ggatgaagat gagttaatca
     8821 atgtcttgtt ggcagaagga gatatggata ttatcattgg taccaagttt ggttatgcag
     8881 ttcgctttaa tcaatcagcc gttcgtggta tgagccgtat cgccactggt gtgaaaggtg
     8941 ttaaccttcg tgaaggagac acagttgttg gtgccagctt gattactgat caagatgaag
     9001 ttcttattat cacagaaaaa ggatatggta agcgtacagt cgctactgaa tacccaacaa
     9061 aaggtcgtgg tggtaaggga atgcagacag ctaaaattac cgaaaaaaat ggcttgctgg
     9121 ccggtcttat gactgttcaa ggggatgagg atttgatgat tatcactgat acaggtgtca
     9181 tgattcgaac caatcttgcc aatatttcac aaacaggacg tgcaactatg ggagttaaag
     9241 taatgcgcct ggatcaagat gctcagatag tgactttcac aacggttgcg gtggcagaaa
     9301 aagaagaagt tgggacagaa aacgaaacag aaggtgaagc ataatgtctc aaaaaaataa
     9361 taaaaagaaa aacaagcgaa aaaatctgct gacaaatatc ctagcaggat ttctgatatt
     9421 actgtcactg gctttgattt ttaatactca aattcgaaat attttcatag tctggaatac
     9481 caataagtat caagttagcc aggtatcaaa agaaaaatta gaagaaaatc aggatacaga
     9541 aggcaatttt gactttgatt ctgtcaaagc tatctcttcg gaagctgttc taacttctca
     9601 atgggatgct caaaaattac cagttattgg gggaattgca attcctgaat tggaaatgaa
     9661 tttgccgatt tttaaaggac ttgataatgt taatctcttc tacggagctg gtacaatgaa
     9721 acgcgagcaa gtaatgggag aaggaaatta tagtctagct agtcaccata tctttggtgt
     9781 tgataatgct aataaaatgt tattttctcc tttagataat gctaaaaatg gcatgaagat
     9841 ttatctaacc gataaaaata aagtttatac ttatgaaata cgtgaagtca aacgtgtgac
     9901 accggatcgt gttgatgaag ttgatgatag agatggggtc aatgaaatca cattagtaac
     9961 ctgtgaagac cttgctgcta cagaacgtat tattgtcaaa ggtgatttga aagaaacaaa
    10021 agattattca caaacatctg atgaaatcct aacagctttc aatcaaccat ataaacaatt
    10081 ttattaatac aaatcagtga aatcatggat ttcactgatt ttttaagatt ttcataaaaa
    10141 tatagtaaaa tgaaataaga ataggacgaa ttgttcagga ca
//
LOCUS       DAVKHZ010000074        10129 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000074 DAVKHZ010000000
VERSION     DAVKHZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.074"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            5..451
                     /locus_tag="VWW99_001740"
     CDS             5..451
                     /locus_tag="VWW99_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9539546.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(589..1893)
                     /gene="eno"
                     /locus_tag="VWW99_001741"
     CDS             complement(589..1893)
                     /gene="eno"
                     /locus_tag="VWW99_001741"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9539547.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2057..2503
                     /locus_tag="VWW99_001742"
     CDS             2057..2503
                     /locus_tag="VWW99_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9539548.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2500..3615)
                     /locus_tag="VWW99_001743"
     CDS             complement(2500..3615)
                     /locus_tag="VWW99_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9539549.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVEYLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTYGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLDLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3624..4272)
                     /gene="serB"
                     /locus_tag="VWW99_001744"
                     /pseudo
     CDS             complement(3624..4272)
                     /gene="serB"
                     /locus_tag="VWW99_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4331..5764)
                     /gene="glgA"
                     /locus_tag="VWW99_001745"
     CDS             complement(4331..5764)
                     /gene="glgA"
                     /locus_tag="VWW99_001745"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9539550.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGFPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5761..6900)
                     /gene="glgD"
                     /locus_tag="VWW99_001746"
     CDS             complement(5761..6900)
                     /gene="glgD"
                     /locus_tag="VWW99_001746"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9539551.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKSAKVTEDIHS"
     gene            complement(6890..8032)
                     /locus_tag="VWW99_001747"
     CDS             complement(6890..8032)
                     /locus_tag="VWW99_001747"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9539552.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8022..9950)
                     /gene="glgB"
                     /locus_tag="VWW99_001748"
     CDS             complement(8022..9950)
                     /gene="glgB"
                     /locus_tag="VWW99_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9539553.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3071 a         2249 c         1786 g         3023 t
ORIGIN      
        1 cctattgaga aaacatgggc tcatatcaaa aagcacctca aaaaggtatt accaagttgc
       61 aatacctttt acgaggctct tttgtcttgt tcttgtttca attgactata taaattgtct
      121 aagcgaaaca accgataaga attggcacaa aagcgaccgt atttttgtta ccaatacagg
      181 aaaaccagtt catagttcta tcttgagcaa gtctctccag cgagcaaacg aacgccttaa
      241 aaaaccaatt cccaaacatc tgtcccctca catcttcaga cacaccacta ttagcatctt
      301 atcagaaaat aaaattcctt taaaaacaat cacggacagg gttggtcatt ccgactctga
      361 agtcactact tccatctaca cccacgtcac aaagaacatg aaagatgaag caatcaatgt
      421 actggataaa gttatgaaaa agatttttta aaaagttttg cccctttttt gtcctctaaa
      481 tacaaaaata gcccttcgga taaaatccga ggggctagaa acgttgttaa atcaacggcc
      541 gaacttttga atttcatggt tcgggataaa atagttcact gaactatttt attttttaag
      601 gttgtagaat gatttcaatc cacgatattc agctacttca ccaagttggt cttcgatacg
      661 aagcaattgg ttgtatttag cgatgcggtc tgtacgtgaa agtgaaccag tcttgatttg
      721 tcctgcgtta gttgcaactg cgatatcagc gattgttgaa tcttcagttt cacctgaacg
      781 gtgtgataca acagcagtgt aaccagcttc tttagccatt tcgatagctt caaaagtttc
      841 agtaagagta ccgatttggt taactttgat aaggattgag ttagcagcac cttcttggat
      901 accacgtgca aggtagtcag tgtttgttac gaagaagtcg tcaccaacaa gttgtacttt
      961 cttaccaaga cgttcagtaa gagctttcca accatcccag tcgttttcat ccataccatc
     1021 ttcaatagtg atgattgggt atttgttaac caattcttca aggtagtcga tttgttctgc
     1081 agatgtacga acagcagcac cttcaccttc aaatttagtg tagtcgtaaa ctttacgttc
     1141 tttatcgtag aattctgatg aagcacagtc aaatccaata aatacgtctt tacctggtac
     1201 atatccagca gcttcaatcg cagcaaggat agtttcaaca ccatcttcag ttccttcgaa
     1261 acgaggagcg aatccacctt cgtcacctac ggcagtttcc aaaccacgtg atttaaggat
     1321 tttcttaaga gcgtggaaga tttcagcacc gtaacgaagg gcttctttaa atgttggcgc
     1381 accaactggc aagatcatga actcttggaa agcgattgga gcgtcagagt gagaaccacc
     1441 gttgatgatg ttcatcattg gagttggaag aactttagtg ttgaatccac caaggtagct
     1501 gtaaagtggg atttcaaggt agtcagcagc agcacgagct acagcgatag acacaccgag
     1561 gattgcattc gcacccaatt tacctttgtt aggagtaccg tcaagtgcga tcatagcacg
     1621 gtcaatagct tgttgatcac gtacatcgta gccaatgata gcttcagcaa tgatgttgtt
     1681 tacgttgtca acagcttttt gtgtaccaag accaccgtaa cgagatttgt caccgtcgcg
     1741 aagttcaact gcttcgtgtt caccagtaga agctcctgat ggaaccatac cacgtccgaa
     1801 agcacctgat tcagtgtaaa cttctacttc aagtgttggg ttaccgcgtg agtctaggac
     1861 ttcgcgagcg taaacatcag taataattga cattttttac tctccttatg agttaaattt
     1921 tttacacctc tataatacct taaaacccct cctttttcaa gaaaaaacgt tatctttgtg
     1981 caacttttcc ttaactttat aaagtaatcg ctttcttttg tctgttttat tctaactttt
     2041 atgatatact gttttcatga cagatttatc aaaacaatta cttgaaaaag ctcatggtgg
     2101 gttaaaaata aatccggatg agcaaagacg ctatcttggt acttttgagg aaagagttct
     2161 tggatatgta gatattgaca cagcaaatag ccctcagtta gaaaaaggct ttttatttat
     2221 tttagaaaac cttcaggaaa aagcagagcc actatttgtg aagatttcac caactatcga
     2281 atttgataag caagttttct acttaaaaga agcaaaagaa actgatagtc aagccaccat
     2341 agtatctgaa gagcatatta cttctccttt tggcctggtt attcatagca atgcaccagt
     2401 tcaagtagaa gaaaaagacc ttcgacttgc ttttccaaaa ctttgggaag ttaaaaagga
     2461 agaaccagcc aaaacatcct tatggaagaa atggtttagc taaatcttgc acatatttaa
     2521 taagtgccca atattggcag ccgtgcgctc cagatagaga ctggcatttt tcaaactatc
     2581 ttctaaaggt tcacttttct ccaaaataga aaagacagct tggatatttt caaatggtag
     2641 gggaggtaaa tcttcagcaa gactaccgca aatagcaata acaggaactc caacaggggt
     2701 tctttttgca acacctatag gcgctttccc agcaaagctt tgactatcaa gtcttccttc
     2761 tccaacaaca accaagtcag catctgaaac tttcttatca aagttgatta agtccaagca
     2821 ggtatcaatt ccagacacga tacttgcctg agcaaaggca cacaaaccac cagcaaggcc
     2881 tccacctgct cctgctcctt taatttctaa tgttgcaggt gagaattttt cataaaaatc
     2941 ttggatcgcc tgatctacga ctgcaaacat agtcggatgt agaccttttt gattgccaaa
     3001 agtgtaagtc gcaccttgat gaccacataa gggactcacg acatctgcta gaatatgaat
     3061 ttgaacacct tcaggaattt tatagcaatt ttctgttgaa acagaagcta agtctaataa
     3121 ggattgaccg gaagcaggca agacatttcc atccctatca taaaattgat aacctaaacc
     3181 agcagcaatc cccagtcctc catcattact ggccgtgcca ccaacaccga tataaatatc
     3241 tttaatccct ttagaaatga gatggagaat caactctcca ataccataag tttggatttg
     3301 aagtggattt cgtttctcta gcggaatttt tccaagacca accaagtcag ctacttcaaa
     3361 tagtgccagt tccccttttt gaaaatagcg catggcttct ttttgtccaa aagggtctgt
     3421 cacttggatc catttttctt ttaggtcaag agaatgtcgg atagcatcta cagtaccttc
     3481 tcccccatca ccaacagggc agaggagata ttctacatct gctatcgatt gttggaagcc
     3541 tctttttatt gcttcagcta cctgttgagc tgtcaagctt tccttaaacg aatctggtgc
     3601 aattacaatc ttcatatttt ccctcattct aaacagtcaa tcaaaggaag aacttctaaa
     3661 aaatccctct tgtcaacatg atgtggtatt tcttttttga gcacttcttt ggcacaaaag
     3721 gcgattccta actccgccga cttcaacatt aatagattat tgaccccatc accgattgcc
     3781 accgttcttt ctttagaaag ttttagtttc tttctccatt tttccagagt ctcttttttg
     3841 acctggggac ttataatttg ttcaactaat tttcctgtta aaagaccttc tttgacttca
     3901 agctggttgg cagtgaaata ggcaatacca agggattttg ctaatctctc caactattgg
     3961 tatccaccag acaccacaca ccagaccaac taggatgcca ttcttttgga gaatagagat
     4021 gaactctggg acatttagcg atagatgaat tgagttgaag acgttatcaa agaccaaaat
     4081 aggaagacct tccaacaagg acactctttt tcttaaactg ctttcaaaga ccaactctcc
     4141 tcgcattgcc cgacttgtaa tctgcgaaat ttccgcctca tgacctgcct ctctccctaa
     4201 aagatcaatc acttcttcta ggattaaggt tccatctaca tccaaaacac acaagccttt
     4261 tacttgagac atcagttctc ctctctaaac agcctaaaaa tcgtatgaag tcatcatacg
     4321 attttatcta ttaattaact aaactatggt acaagtcaag gtatgacttg caggctgtat
     4381 cccatgagaa gtcactctcc atagcttgtt tttgtaggtt tctccaaatg tctggatggt
     4441 ttctatacaa gtccaatgct gtttggaaag tccaatttaa ccaataagga gatagattgt
     4501 caaagctaaa gccagtaccg cttccttcga ttggattgaa agcgcgaact gtatctcgca
     4561 agcctccaac ttcatggacc aatggcaagg ttccataacg catagccatc atttgagaca
     4621 agccacacgg ttcaaaacga cttggcatga ggaagaggtc acaagcagcg tagatttcct
     4681 gagcaagttt gacatcaaaa gtgatatttg ctgatagctt gtctgggtaa atctgagcaa
     4741 accatgagaa agctccttca aaggctggat cgccagttcc caaaagaaca atctgaacat
     4801 cttcttgcaa gatatggtga agactttcga ccaccacatc aaaacctttt tgacgtgtca
     4861 aacgagaaac aattcccacc agtggaacgt cagctctaac aggaaagcca actctttctt
     4921 gcaattttgc cttatttttg gctttcccag acaaatcttc ctgattgaaa tgatagtcta
     4981 aaagagcatc cgtctgagga ttataaagat cagcatcaat cccattcacg ataccagata
     5041 ctttaccaga ctccatttta agaatctgat ccaaattaca tccaaactga ctagtcataa
     5101 tttcatgagc atagctaggt gaaacggttg aaacacggtt cgcatagaga atacctgcct
     5161 tcatccagtt cagacagttg ttccatcgaa gggtgccatc agcgtaacgt tcaaagccaa
     5221 ctccaaacaa atcacccaac attccttctg aaaattgtcc ttggaattct aaattatgaa
     5281 tggttaaaac tgtttcaatg tcctcatagg cttgaatcca acggtatttt tccttcaaca
     5341 agaaaggaat catagctgta tggtagtcat gaacatggag aagatcagga ataaagtcaa
     5401 tcctttccat agcctcaatg gcagccagtt ggaaaaaggc aaagcgttct ccgtcatcaa
     5461 aatcaccgta aacatgacca cggaagaaat aatattgatt gtcaataaag tagaaggtta
     5521 caccatttaa tactgttttc ttaattccac aatactgtct gcgccaacca acgctcacct
     5581 caaaatgaag cacatcttca atctgatttc caaatttagc ctctaccata tcatagtagg
     5641 gtaaaatcac tgcaacttcg tgcccagctt ttaccagtga ttttggaaga gcgccaatga
     5701 cgtctcccaa accacctgtt tttgaaaagg gtgcaccctc tgctgctaca aataaaattt
     5761 tcatgaatga atatcctctg ttactttagc acttttctta accacaactg gatgttctgc
     5821 agttcctcga atcacaacac caggctcaac ttcaacccct ttgtccaaga tagcatattc
     5881 gacctgagcc ccttctccaa taacaacacg agggaagagc aggctatctt taaccaagct
     5941 atccttatga acatgaatat tacgtgatag aacagaatta gctacttgac cttcaataat
     6001 actaccagag gcaaactgag aagtgcttac cttagatgta ttagcatagt aagttggctc
     6061 ttcgtttttg acctttgtat aaatcttttg gtttggtgag aaaagagaat agaatttttg
     6121 tgattcaagc atatcgatat tcgcttgata ataagattta acagagtgaa tattggctag
     6181 atagcccgtg tactcgtagg cgaaagctcc ctcttttaca gccaaatccc gtaaaacata
     6241 gcgcaatttc tctggatgtt cttttttagc ttcttcttcc aagtgttcaa tcaaccaagg
     6301 tgtatcaacg acaaagatat ctgtagacat attgaacgtt tcagctgttg acttgctatc
     6361 aaagagttta tgagaaagaa catggtctgt ttcatctaca tccaagattg catttacttc
     6421 tgaaatatct ttcttagcta gttttttata aactacagtg ataggctctt ttgttgtact
     6481 atgtaggtgg aaaacttggt tcaaatcaat gttaataaga acatcgcagt tgagggcaac
     6541 cgtttggttt gagccagaac gtttcaaata agtaagaagc tgttggtagt attcttttcc
     6601 aactgtacta ctttctacac gggtattgta aattcctaga tagtaatggc taagaagggt
     6661 tgataagccc cactcgcgtc ctgaacgaat atggtcaaat actgagctga tattatcctg
     6721 ctggaaaata ccaaagacac tacgaacacc tgcattagca aggcttgaaa gtgggaagtc
     6781 aatcaaacga tatttcccac caaatggcaa acttgctact ggacggtggt ccgtcaatgt
     6841 cgacatattg tgaaaaccaa ctgtatttcc taaaatggca gaatatttat caatcttcat
     6901 ctgttgctac ccccactact tcattatatc ctacaacttg tacttcatct gttccatcaa
     6961 tttcgacacc gtcagaaata atcgcacctt caccaataat ggcacgttta atcttagctc
     7021 cttgaccaat gatagctcca ctcatgataa ctgaatcaag gacttccgct ccttcgcgaa
     7081 cttgcgcgcc tgttgaaagg atagaatgtt taacagttcc atcaacgaaa catccgtcta
     7141 caactaatga gtcttccaca tgagcatttg ccccgaggaa gtttggtggt gaaatcaagt
     7201 ttcttgagta aatcttccat tgacggttac gactatccaa ggcattttct ggagaaatat
     7261 actccatgtt cgcttcccaa agtgactcaa tagtaccaac atctttccaa taaccactaa
     7321 attcgtaagc ataaacactt tcacctgact caaggtaatt tggaatgaca tttttaccaa
     7381 agtctgacat gccaaccttg ctcttttcag cagcgactaa catattacga aggcgttgcc
     7441 aatcaaaaat gtagattccc atagaagctt ttgtagattt aggttgagct ggtttttctt
     7501 caaattcaac aatacgattg ttagcatctg tgttcatgat accaaaacgg cttgcttctt
     7561 taagagggac gtctaaaact gctactgtca agctggcatt attatcctta tgagactgga
     7621 gcatatcatc atagtccatt ttgtagatgt ggtccccaga caaaatcaag acatactcag
     7681 gattgacact gtcgatatag tcgatatttt ggtaaatagc gtgactagtc ccctcaaacc
     7741 aacgatttcc ttcacttgca gaataaggtt gaagaataga gacacctgaa ttaataccgt
     7801 ctagtcccca gcttgaacca ttcccaatat ggttgttgag agcaagtggt tgatactgtg
     7861 taacgacccc aacattgtga atccctgagt tggcacagtt tgatagggca aagtcaatga
     7921 tacggtagcg cccaccaaat tgcacagctg gttttgcgat gctttgagtg agtttaccga
     7981 gacgagttcc ttgcccacca gcaagaatca aagctaacat ttcatttttc attttctact
     8041 cctttttggt ttttatttgt gacggtttta gtagatttca agcgacgttt gattttccat
     8101 acacttgctc ccatagccgg tagggtaaag gttaaggtct gctcataatc tttccatagt
     8161 ccttcttgcg tttgaacagt ttgattatgt tctttccaaa cgcctcccca ctcttccaac
     8221 tcagtattcc atacttcttc gtaaattcct gcaacgggta gtccgattgt aaaatctttc
     8281 cgctcaacag gtaccatatt aaagatacag actaacattt ctcccttttt acccttacga
     8341 ataaaggaaa gaacactctg gtctcgatta tccgcatcaa tgatttcaat accatcatag
     8401 ctggtatcaa tttcccacag acagcgatga tctttgtaaa actggtttag ctgagaagcg
     8461 aaatacttca tcttagcatt cattgggtct tctaggttag accattccaa ctgttcttca
     8521 gatttccatt ctaggaattg accgtattcg ctacccatga agagcaattt cttaccaggg
     8581 tgacaaattt ggtacgtata gagattgcgc aagcctgcaa attgattgta acgatctccc
     8641 cacatcttat gcatcatact cttcttgcca tgaaccactt catcgtgcga gaatggcaag
     8701 agataattct ccttgaaaac atacataaag ctgaaagtca ccaggttaaa gtcatattta
     8761 cgatagatcg gatcttcttc gtagaaacgg aggatatcat tcatccagcc catgttccat
     8821 ttgtagtcaa atcctagacc accaatctct ttcattcccg taatcttggt cgcagacgaa
     8881 ctttcttctg caatcatcat cacatctgga tattctaact taataacctc attcaagcgc
     8941 tgaaggaaat aataaccttc atagttgaga tttccgccat ctttattagg tgtccatgga
     9001 gcatcatcat agtccaaata gagcatgttg ctaacagcat ccacacgaat accatccaaa
     9061 tgatagacat caatccaatg cttaatgcaa gaaattaaga aggactggac ttcatttttt
     9121 ccaaggtcaa aattaagggc accccaacca tggttatgag ccttattatg gtcttggtat
     9181 tcaaaagtcg gtgtcccatc ataataggct aaggcatcat cgttgatggt aaagtgacct
     9241 ggtacccagt ccacaataac cccaatatta tgggtatgac actcctcgac aaaatcttga
     9301 aactcctctg gtcggccata agcatgctct aaagcgaagt aacccataag ctgatacccc
     9361 caactcaagc ccaaaggatg ggacatcaag ggcataaact caatatgagt atagttcatc
     9421 tcaactaggt aaggaatgag ttcatctttc aattgggcaa aactataagg actgccatca
     9481 ggatttctct tccaagagcc agcatgaacc tcgtaaatat tgacaggacg ttcagcaaag
     9541 ccccaacgtt ttcttcgtgc cagccaaagt ccatccctcc atttcttatc aggaagctct
     9601 gttacgattg cccctgttcc tggacgagcc tcatacctga cagcaaaagg gtcaatcttc
     9661 atcagttgat gaccattttg acgtgtgaca tgatatttgt aaatatgccc ttcttgagcc
     9721 atattggtaa agacttccca gaccccaaag tcatttctta ccattggaat ctgattttca
     9781 atccaattgg taaaatcacc aaccaagtga acagcctgag cattaggtgc ccaaacacgg
     9841 aaggtatagc catactctcc atttagttct tccctatgtg ctcctagata atgttggaga
     9901 taaaaatttt cacccgtcat aaaggttttt aatgcttctc tattatccat atactccctt
     9961 tctcatgtaa gcgttttcta tgtttttatt atactacctt tttagagaag attcaagtaa
    10021 attactatac ttctttaatt attttgaaaa tctacaacaa gttcacttac tcgttcaatt
    10081 gtaaatcaat attttttcaa aaaattgcga aaacaccttt ctttttcta
//
LOCUS       DAVKHZ010000075         9911 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000075 DAVKHZ010000000
VERSION     DAVKHZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.075"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            108..407
                     /locus_tag="VWW99_001749"
                     /pseudo
     CDS             108..407
                     /locus_tag="VWW99_001749"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(685..1455)
                     /locus_tag="VWW99_001750"
     CDS             complement(685..1455)
                     /locus_tag="VWW99_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9539554.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(1470..2318)
                     /locus_tag="VWW99_001751"
     CDS             complement(1470..2318)
                     /locus_tag="VWW99_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9539555.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGTPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(2315..3790)
                     /locus_tag="VWW99_001752"
     CDS             complement(2315..3790)
                     /locus_tag="VWW99_001752"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9539556.1"
                     /translation="MVQTKQPNIILIVVDQMRADVLSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(4113..5468)
                     /locus_tag="VWW99_001753"
     CDS             complement(4113..5468)
                     /locus_tag="VWW99_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9539557.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(5478..5795)
                     /locus_tag="VWW99_001754"
     CDS             complement(5478..5795)
                     /locus_tag="VWW99_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9539558.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5812..6129)
                     /locus_tag="VWW99_001755"
     CDS             complement(5812..6129)
                     /locus_tag="VWW99_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9539559.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(6144..7040)
                     /locus_tag="VWW99_001756"
     CDS             complement(6144..7040)
                     /locus_tag="VWW99_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9539560.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(7037..8305)
                     /locus_tag="VWW99_001757"
     CDS             complement(7037..8305)
                     /locus_tag="VWW99_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9539561.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGTVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLEDCE"
     gene            8585..9763
                     /locus_tag="VWW99_001758"
     CDS             8585..9763
                     /locus_tag="VWW99_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9539562.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         3446 a         2078 c         1421 g         2966 t
ORIGIN      
        1 agtttctata gtaaaatgaa ccaaaaatag tacacgttgc ggtataatgt tcttgtggca
       61 tattcattag attttcgtaa aaagttctct cctatttgtg agcgaacagg tatagtcaat
      121 tgattgtcag tttgaagacc aagagtcgta aggcaaagga tatggcagaa tccatccaag
      181 ggtggctggc acaatttcta gtgaacctct ttaaatctat tacgtttgac tgtggaaaag
      241 agttttcaaa atggaaagat atttccaatc atcacgatag tgagagcttt tttgcgaatc
      301 taggatgtcc aagacagcgg tgtctcaacg aacattcaaa tagactttta aggtgtcatg
      361 acttgccaaa gcagacagat ttcaacgaag tcagtcaaga attttaggct gggtaaaaac
      421 tcaatttcag gagaaaatga aataaatatt cccacaataa aacgcatagt atcaaagttt
      481 ttccaacacc tgatattata cattttttaa ttttaaagac tttttcccag cctcaagaaa
      541 tcacatccca cctaaatcct ttggatacca aatacctcat caggtatttt ctgagttggt
      601 aagactgtct agcttaattt gacaaatgga gggcgttaaa tccagttttt acaggaagtc
      661 tatttaaata ttttcacact gaccttacaa aatatgaaat aaattatata aagtcattcc
      721 catgacaaag aataacatcc ctttaaaaca gtatcttacc caattttcag gtaagacttt
      781 tgatacaacc gtccccaata cccctccgca tatagaagca agaaaaataa ctggaacagg
      841 tgctaaatga tactgattta aacctgtaac cacaacaatt gtagccaaat gagacaactg
      901 ggaaaagaaa ataattgcaa gagaatacat cgttgcttct tttatcgaaa tagaaaaaaa
      961 caccatcaat aaagatacat tgagcggacc accaccaatc cctaaaaaac tagaaatcaa
     1021 gcccaataga agaccacaaa tcatatagaa tcctatctta tcaaattgat aagttttttt
     1081 aaaatctgtt aaaacaaatg atacaagtag catcacaaat agtaaaatca tttgaacaat
     1141 ggatactagc cgtactgata actgagataa caaaacttga aaaataagat gacctagaaa
     1201 tcctcctaaa acagaagaaa aggataccgt aaaaatcaat ctccatttta tctctttact
     1261 ttgtgaaaat cttttgattg ttgaaataat cgccattata aaaacagaga aactagaata
     1321 aaaagcaata tcactgaccg aatggtatcc aaaactatcc ataagtggct taattataat
     1381 tccaccaccc atgcctgata tagaacccaa tccactagct attataatga ttagaaaata
     1441 aataatctca accacttttt aagtccatcc tattctttca aacatctaaa acctagatgg
     1501 ctactagtgg aagtagaaat acatccattt ctagcagcta ctcggtatcg attacaataa
     1561 gaacaatgac ataaaaagga acctcctctg atagcatact cctcatcttg tatcccatat
     1621 tttggtaact catagtcatc ttcattaaaa ctagctaata aggtatatct cggattgcga
     1681 caccattccc aaacattccc aatcatttga tatagaccaa aatcattagg taaaaattca
     1741 tagacaggag ccgttcctat aaatccatct aaggcagtat tttcataagg aaatcttcct
     1801 tgccaagtat tggcatgata atatccacct tctaaaagag tatctcccca aggatattca
     1861 gattgtcttc ctcctctagc agcatattcc cactgtgctt cagtcggtaa agacattccg
     1921 ctccaattac agaaagccaa tgcatcttct aaagcaacat gaactactgg atggtcttct
     1981 aaacccacta gattactatt ctcaccgtag ggatgtttcc agcaagcgtt cggaacttgt
     2041 aaccaccacg gagtcccagc aggatgagga tatccctccc tttctgcctc tggaacgaac
     2101 aatataaaaa caaaagacca ctcttgtcgc tcagctagtg ttttatagcc tgtttctttc
     2161 acaaattgag caaattcttg attcgtaact ggagtatccg caatggaaaa atcctttaca
     2221 ctaacagcga cttgaggacc ttcaaaatca tctaaaaagc cctcttcaga atttgttccc
     2281 atcataaatg tcccaccctt gatttttttc attttcatga ttgactatcc cccgttttcg
     2341 ttagagttgg cgttatttta gaaagttcaa caggaactaa ctcttcatcc acaacaaaac
     2401 cttcttctct atatctaaga aagtctacta attttttctt catctgacga acaataggtt
     2461 ggtatttttc acttggatac aaatcattca tttcatgcgg atcttttttc atatcaaata
     2521 actgataatg atttaaaaca ggaaaccaga taaatttcca ctggtctgtc aagatatatt
     2581 gactagaatc ttttcctaaa gcatgttcac catgaaattc tgttcgccac ccctcgtact
     2641 gtccaaataa taaattctta acactccgtc catctagttc atctgtagta gttcctcctg
     2701 ccaaatccac gagtgaagga aaaatatctt gaattttcac taattgcttg attgttcccc
     2761 tattaccagc tatcaatcca gccggatcgt aaataaacga aggaatgtgg atacttccct
     2821 gataaggata tccctttcta aacagataat gttcaccaag ctgatctcca tgatccgaaa
     2881 caaaccagat aatcgtatct ttatcatgac gaaattcttt tagggcagtc aaaaagcgac
     2941 taatttgatg gtctatatga gtaatcaatc caaaataagc agcaaccatt cttctttgat
     3001 catcttcctt taatttccct cttaaagcat aaatagacgg aatttgcttt tctaatactt
     3061 cccaattccc gatatgcaaa tcgagaaatt gaggcaagcg ttccatatac atatcaaaat
     3121 agtattttgg gggatttaat ggagcatgtg gtttttcaaa agacattttg agaaaaaaag
     3181 gaaccgttgg atctcttctt tgtaaaaatg aaatggactc actcacaacc cagtttgttg
     3241 gatgtaactt ctcatcttta tcccaaggtc tagcttccca agaattacaa tccattccgt
     3301 catcaatcaa atcaacatca taaccaactt taccctttaa aaaggctaga tagtcactag
     3361 cataatcaaa ttgacttcca taagccttat catactttct atcaacatgt agatacccat
     3421 cgtgaagtaa gacatgatca aacccaagtc tctgtctgga aggaaaaaca tgcatcttac
     3481 caatacactc tgtttgataa cccatatctt taaagacttt tggaagagta ttagtaaaat
     3541 tccaaggcac ctcatcttga taaccaactc gtccactttt atcttggtct aagccagtca
     3601 ataaagctgc cctagcagga acacaagaag gaactggaga ataggcattt tcaaaattat
     3661 aacctacact agccatcata tctaatgttg gagtgctaac tagcttatcc ttactattca
     3721 aggataagac gtctgctctc atttgatcta caacaatcaa aataatattt ggctgttttg
     3781 tctgaaccat aaaatctcct ttctaatatg gcaaaagagg cacaagaaga tatctacctt
     3841 tactgcaccc ctttctatat caatctctct atataaaaca ataacattct tgttatgttt
     3901 tatagaaaaa tgaactaaaa tatgactaaa tcgattagga aattcaaatc attttctagt
     3961 actgttttag aagttacagt gtactattcc aacttcaata aattataaac ctttgtctaa
     4021 taacaatttt agtggagata agaaatccta cacctaactc atcttacacg taatctattt
     4081 ctattttatc acaaaaaacg caagtaagac cattaactca attcagtttt atctgccatt
     4141 ttcacaaatg ggaaataagt caagacacta ataatcaaac aaacaactga taagatgatg
     4201 gcacgccaat caaatgctgt agagaagaaa ccatataaaa ttggaggcat tacccaagta
     4261 acgttttgtg taacaggtga aacaagaccc cagcttgttg cccagtaagc taccgttgcc
     4321 atgaaaaccg ggctaagtac aaatggtata aatagcaaag gattcaagac aactggtaaa
     4381 ccataattga ccggctcacc aatattaaac agaactggtg ctagaccaag tttagcaact
     4441 tttcgataat gactgtttct tgaaaaaatt aaaatagcaa gtactaatcc taatcctcca
     4501 aaccagacaa acgccccaaa agacccactt gtccatatat aaggaatcgg ttcacctttt
     4561 tggaaagcat ccagattcgc taacatagca actccaaaca gcccttccat gatgggagcc
     4621 aatacatttc ctccatggag accaaaaaac cagaataact tattcaaaaa gatcatcaga
     4681 ataactgcaa agaaactttg agacaaacct agtaatggcg tttgtaacac cttgtaaacc
     4741 caatcaatca ataagtcatt gctaagtaaa tggaaaacat aagtcaagat ggctactata
     4801 tacatcgcca taaatcctgg aatgatagaa gtgaacggct tagcaatcgc agggggaact
     4861 gaatctggta acttgattac ccagttcttt ttcattactt tacagaaaat aatagaggct
     4921 aaaaatccaa tcatcatggc tgtaaagtag cctctggcat taatatggtt tcctggaatc
     4981 acattcccaa tagttaccat cagattttta ccatcaaatg ctagattatc aattccttgt
     5041 aagatttgat ctaatttcac atctcctaca tttgccaaag ggaaactctt tgtaactgta
     5101 cttccaatcg aaatgacaaa cgaagcaagc gataccaaac cagcagaaac tgtatcaacc
     5161 ttataaatct tagcgatatt cactcccaag caatagatga acaataagga aacaattggt
     5221 atacttccct tgaataccaa attattgatg tcaacaagcc actgaaaggt tttcgtaata
     5281 ctttctaggt gaaattgttg tggtaaatcc actagaaaag catttaataa caaagcaatg
     5341 gaacctgtca taataacagg catagtcccc acaaatgaat cacgtaaagc aactaaaaac
     5401 ttctgattcc caactttcat tgcaattgga gaaagttttt cttgcatttt atccaacatt
     5461 gtattcatag caaattccta atttttttca aatcgttcgt atacttctac cacttctttc
     5521 gccaagtcaa caaaggtcag actcgtcatt aaatgatctt gcgcgtgtac catcaagagg
     5581 ctaattgatg tttcttcccc ctgtgcctct tttgtcaaca tttctgtttg agccttatgc
     5641 gcctgtaata aggcagcatt cgcatcagct aatcttcgat tggctttaga gaaatcccct
     5701 ttttttgctg cctgaatagc ctccatagca ttacttttgg cttccccacc atacataatt
     5761 agccccattg cagattctaa attactttca ttcatagcat tctcctcaat cttagtctaa
     5821 taaactttca gctaaatcta agactttaga accattcatc attccataat ctgtcatcgg
     5881 aataaccgcc acaggaatct gtctatcttt tagtttttct tgaaagtccc ctagtaaata
     5941 gcgaacttga gggcctaaaa gcaacacatt tacttctttt gttgctacaa tttcttctgc
     6001 ttcaggagct ggaactgcaa aaatttctgc atccaacccc ttatcttctg ctgccttttg
     6061 catctttgtc actagcagac ttgtactcat acctgctgca catgctaaca taattgttac
     6121 tttagccata gcttactcct ttatcaccta tttactgacc tagccaagcg agatactgta
     6181 tgtctcactc ctctaattgc cttgctcaat gcataaatat tatcgactgt tgccagtccc
     6241 ccatatcctg cgtcacctat atgttgaata tcaactccac aaattttatt tctaagtgca
     6301 atttccttaa tagtatctgt atcagatgtt tcttggctag taccaatagc actcaataca
     6361 agaccaccct tactatgaac gagatcaacg acttcacgca actcttggtc atgaaaggct
     6421 ggaacggttc caactgctgg aacaagtatc acatccgcac ctgcttccaa caattgctct
     6481 gcgacagaaa gctctgccac aggctcattc actcctgccc cgtgcatctt acctgcaata
     6541 atcaaaccag aaaagttttc cttagcagtt tgaacagcct taatgatttc tcgattgcta
     6601 actcctgttc caggatttcc agtcaaacag acaaagtcaa aacctaactc ctctattcgc
     6661 ttcaatgttt caacactagc aacacgacct gcaacaattt cctgtgtttc ctctagcatc
     6721 tttgcagatg ggtcaatcgg ttccaaattt acaccaattg gacaagcaac aagctggtgt
     6781 aagcgatgaa taacatcatc acctgaacta tccaaagcat aaattttagg ctcaaaaaca
     6841 tcaacacaat tcaataaaat catatcagct ccaaaagctc tagcaatttc agaattagtt
     6901 atatccccaa caaatgtctc acgagtaact acattttcag atagaacaac tctaccttca
     6961 cttgctaaaa tactttgttt taattcttca gcattcatct gtaatatctc agatggattt
     7021 gcactaatta aacgtttcat tcgcaatcct ccaataactt aaaccgtttt tcaatagcta
     7081 ttacaaatgg taaataaagt aagacatcca aaacaatcaa tacaatttgt aataatgaac
     7141 cagaaatact tcctgtagca agtaaaccac ttataacagg tggcatagtc caacctggat
     7201 ctgtataagt tagcggaacc aaacctgatg ccattgctcc ccacgctact agtaagttaa
     7261 acattggagc taagataaat ggaactagta acaaaatgtt caaaacaata ggaacgccaa
     7321 acattgctgg ttcatttata ttaaataatc caggtataac agttataggt gccaaagttt
     7381 ttagttgttt actagctttc ttcttatgtg ctaaatatcc taacgcaaga actaaaccta
     7441 tggttgcacc accgccacct atgaatacaa aattatccat aaatacgcta gtaaaaatat
     7501 ttggaagagt ttctccagtt tgataagcta ctttatttgc atctagatta gctaaccata
     7561 acggtttcat aactgcatta acaacatttc ctccgtgtaa tcctacaaac caaaaactac
     7621 tattaaggaa aattaagata agaaggccaa taacattatt tcctaaaagt ccaagtggac
     7681 ctcccaaagc tacttgagca atttcatgaa gattaggtaa acttaattta tctaaagttg
     7741 cataaacaat caaccaacca acaatagtca ctgtaccagg aataatggca ctaaagcttt
     7801 tagatactgc tggtggaaca ccatccggca tttttatctg tatattatga tttataaacc
     7861 attggtagat atagccatta atcaatccta aaatcatagc aacaaataaa ccttttgatg
     7921 ccatgaaagc ggtaggcatt cctgctccag cctctcctct aatgaaaggc gtaactgtaa
     7981 taaaagatga taatgacaca ataccagcag gaataccatc aactttgtat tgtctagcca
     8041 taaagtaagc gataccaaaa ctagctacaa gaccaagtaa tccaaaacta ctatcaacac
     8101 ccttccataa atagtcagca acaccaatat cacccagata tttttcccat cctggaatag
     8161 gaaaacttgc aataaccatt agtaatgaac ctatcaacaa taaaggtata gttagagtaa
     8221 taccgtctcg aatagaaacc aacgctttat tattacccaa tctagccgct actggaagta
     8281 acttttcttg taataatttt tccataacct tttcctctta aatatatttt attatcaaaa
     8341 gttatcaatt atctttattt tttgacgtca agttgttatt tgattatctt ttgttattta
     8401 tattttacaa ctataagaaa gccttgtcaa ggatttgtgt aaacatttga ttatttttta
     8461 ttatcaatat ataataaaaa gagaccagta agtaatgaaa agcttaaaaa gattaacatc
     8521 tacatttatc catagcaaaa aatcatagga ttttaagaat ctgacaacta ggaagaaaac
     8581 taatatgacc ttatctaaga aacaattaca actaagagct aaaatactgg aaacagttta
     8641 cacattaggt cccatttcac gaattgaaat cgctactaaa acaggaataa cacctgcaac
     8701 aactagcagt atcacaaatg atctgattaa agaaaatatt ctcttagaac ttggtgaaga
     8761 cgaacacgat acaagcgtag ggagaaaaaa aatcctgcta gacattcaag ctaaacgatt
     8821 ttactacatt ggttgtgaac tatctgaaaa acatttcact tttgctttag gagataatct
     8881 aggaaatatc ttaaaggaag agaaagaaat tgttactaaa cagttaatcc aagaaaaggg
     8941 aaatcaactc attaaccaga cactaaagca attcttaaat aactgctctg attatgaaat
     9001 agaagcaatt ggaatcgcgc tacctggtcg ctatttagat aattataaaa taacaacaaa
     9061 caatccttta tggcaacata tcgacttaga aatgattcaa tcccactttg ataagccttt
     9121 gtttttctct aacaatgtta actgtatggc tataggtaaa cgcctattta gtcgccaaca
     9181 gaatgaccct aattttgctt attttcattt tgctagaggt atgcactgtt cttatatcta
     9241 cgacggaaac atttatggga aaggaaatct aatgattgga gaaatagggc atacagttgt
     9301 ttcatctgag ggagaagaat gtagctgcgg gcgcaaggga tgcttacaaa cttttgctgg
     9361 agaatcttgg ttaatcaaaa aatcaaaaat tctatatcat caatctccct attctctact
     9421 tcctagttta gtcaaaaatg ccgatgatat tgatatccaa gttattttaa ccgcttatca
     9481 attaggtgac actggaataa tcacacttat ccatcaagcc ttactctatc ttagtcaaac
     9541 cattttaaat atcagtatga tgattgactc tcaaaaaatc tacctacata gtcctttact
     9601 aacaaatcaa cacatcatcc aaaaactata tagtgaaatg aactacaaac ccaaactgct
     9661 ctataatcgc ctacctgaag tcattattga accttacaat gattttacag ccgcacactc
     9721 agctattgca ctttgtttgt atcatacaat tctacattct taattttcta ttacatttaa
     9781 taaataggct aaaaagaggg agtatatcat aaaaagcgaa taaacctgtt ttctgaaaat
     9841 cagaattctg ttttgttcgc tttctttata tttggaatta tactcctatc tccccttttt
     9901 cataatgtat a
//
LOCUS       DAVKHZ010000076         9682 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000076 DAVKHZ010000000
VERSION     DAVKHZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.076"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..885)
                     /locus_tag="VWW99_001759"
     CDS             complement(<1..885)
                     /locus_tag="VWW99_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU9539563.1"
                     /translation="MKKIKEYEILKVIAIILVVVGHSTYYNIYTGYGGIDYQQYINNH
                     LSLAVYKLLSKLTEIIYYFHMPLFMAISGAFFSIQVKNNRWHGIGSLLTNKFRRLMIP
                     FLVFTLVYTIPIKYISNYFDGISFWKAVSGQLFLFGNSHLWYLYALFIVFIVGFYILK
                     KRTLFYVYLLLYILHILSYSVHLTLVSAPLQYLFWFSMGFLFEFKREQYNHYLKNNKW
                     LSFLLVLLFIACVALKFVLKDNHEVLNRIIMDLAAVLGSLICYNISYYLSDKRELTEN
                     RLFNVILINGLGIYIFSDT"
     gene            complement(951..1846)
                     /gene="glf"
                     /locus_tag="VWW99_001760"
                     /pseudo
     CDS             complement(951..1846)
                     /gene="glf"
                     /locus_tag="VWW99_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272499.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(1909..2760)
                     /gene="rfbD"
                     /locus_tag="VWW99_001761"
     CDS             complement(1909..2760)
                     /gene="rfbD"
                     /locus_tag="VWW99_001761"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU9539564.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATKDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            complement(2826..3875)
                     /gene="rfbB"
                     /locus_tag="VWW99_001762"
     CDS             complement(2826..3875)
                     /gene="rfbB"
                     /locus_tag="VWW99_001762"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU9539565.1"
                     /translation="MTKYKNIIVTGGAGFIGSNFVHYVYNNFPDVYVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDLRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILIKGQIGETYLIGADGEKNNKEVLELILKEMGQTAD
                     AYDHVTDRAGHDLRYAIDASKLCDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(3885..4481)
                     /locus_tag="VWW99_001763"
     CDS             complement(3885..4481)
                     /locus_tag="VWW99_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU9539566.1"
                     /translation="MTDNFFGKTLAVRKIDAISGMLEFDIPVHGDNRGWFKENFQKEK
                     MVPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADNGKVLGTWVDLREG
                     ETFGNIYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYS
                     DPELGIEWENLEEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(4482..5351)
                     /gene="rfbA"
                     /locus_tag="VWW99_001764"
     CDS             complement(4482..5351)
                     /gene="rfbA"
                     /locus_tag="VWW99_001764"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU9539567.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFIIGEKFIGDDSV
                     ALILGDNIYHGPGLSKMLQKAAQKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PKDPRSNYAVTGLYFYDNDVVEIAKGIKPSARGELEITDINKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLELAQPLKKN
                     EYGRYLLRLIGEV"
     gene            complement(5379..6479)
                     /gene="glf"
                     /locus_tag="VWW99_001765"
     CDS             complement(5379..6479)
                     /gene="glf"
                     /locus_tag="VWW99_001765"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEU9539568.1"
                     /translation="MYDYLIVGAGLSGAIFAHEATKRGKKVKVIDKRDHIGGNIYCEN
                     VEGINVHKYGAHIFHTSNKKVWDYVNQFAEFNNYINSPVANYKGSLYNLPFNMNTFYA
                     MWGTKTPQEVKDKIAEQTADTKDVEPKNLEEQAIKLIGPDIYEKLIKGYTEKQWGRSA
                     TELPPFIIKRLPVRLTFDNNYFNDRYQGIPIGGYNVIIENMLKDVEVELGVDFFAHRE
                     ELEVSAEKVVFTGMIDQYFDYKHGELEYRSLRFEHEILDEENYQGNAVVNYTEREIPY
                     TRIIEHKHFEYGTQPKTVITREYPADWKRGDEPYYPINDEKNNAMFAKYQEEASKNDK
                     VIFCGRLADYKYYDMHVVIERALSVVEEEFSN"
     gene            complement(6481..7905)
                     /locus_tag="VWW99_001766"
     CDS             complement(6481..7905)
                     /locus_tag="VWW99_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEU9539569.1"
                     /translation="MKVLKNYAYNLSYQLLLIILPVITTPYITRVFSSDDLGTYGYFN
                     SIVTYFILLATLGVANYGTKEISGHRKEIRRNFWGIYTLQLGATILSGTLYAILCLSL
                     PSMQDSVAYILGFSLLSKGLDISWLFQGLEDFRKIIVRNITVKLVGVISIFLFVKSAK
                     DLYLYVFLLTIFELLGQLSMWVPAREFIGKPHIDINHAKKHLKPVILLFLPQIAISLY
                     VTLDRTMLGALASKTDVGIYDQALKLVNILLTLVTSLGSVMLPRVSSLLSQSDYKSVK
                     KMYQMSFLIYNSVIFPIIAGMLIVNDDFVQFFLGRDFQDARYAIAIMMFRMFFIGWTN
                     IMGIQILIPHNKNKEFMISTTVPAIVSVGLNLIFLPNLGFIGAAIVSVLTEALVWGIQ
                     LHFTWSYLKEVPIIGSMIKIVIASGIMYAFLVSMKSVVHVSPAINVALYALLGGVIYV
                     ALILLFKVVNLKELKQELLKNREI"
     gene            complement(7902..8699)
                     /locus_tag="VWW99_001767"
     CDS             complement(7902..8699)
                     /locus_tag="VWW99_001767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539570.1"
                     /translation="MSYNYAGLNIAPGIIEERGVNWTRYGAVRLQALFLDGYVLAYLL
                     CKLLISDSKNKVSYSLLLLITLFYEGVIYASRSQLIGFSIMFIAIYLLKNKNLLNKLL
                     SFLSLSLASFAILLSPYYEKFIDSFSVSNSDYGAGTMVRIVGRRYYQEIWNQNKLLGF
                     GPISDGNIFAGWKYYLSDLGIIRMLYQFGIIGFIICLLPILYGCLVGFKNRLHFKGML
                     LFCLSLFVLVTSFASQNLYDYNRIMLLPLLLGLANAVSSTQADKDMV"
     gene            complement(9119..>9682)
                     /locus_tag="VWW99_001768"
     CDS             complement(9119..>9682)
                     /locus_tag="VWW99_001768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9539571.1"
                     /translation="GIAGKFFRTSLINDIQLRFNTQLSFAEDILFNFTLLTASRHVIL
                     SPVNFYHVNGTHSLMYYNEKNLKGQIVFVDRIRQILKGYSESSEIVLIENFIILKAMT
                     VYIDRYFGPLWLNGTYSLNEASKLMKSTIEDNDFSKAFNSNRLDYSIGNRYVVFRKLL
                     RFRQYKLCLLYNRIMDKIKGYERFRKQ"
BASE COUNT         3160 a         1977 c         1538 g         3007 t
ORIGIN      
        1 agtatcagaa aagatgtaaa ttcctaatcc gttaatcaaa ataacattaa atagtctatt
       61 ctctgttaat tctcttttat cactgagata atacgatata ttataacaaa taagtgagcc
      121 tagcacagct gctagatcca taattatcct attcaaaact tcatggttat ctttaagcac
      181 aaattttagg gctacacatg ctataaatag tagcaccaat aaaaagctta gccacttatt
      241 attctttaaa taatgattat actgctccct cttaaattca aataagaagc ccatagaaaa
      301 ccaaaagaga tattgtaatg gtgcacttac taatgttaaa tgaactgagt aactcaagat
      361 gtgtagaata tatagcagta gataaacata aaataacgta cgttttttca gaatataaaa
      421 gccaactata aagacaataa acaatgcata caaataccac aagtgagaat ttccaaatag
      481 aaataattgt ccagagaccg ctttccagaa agagatacca tcaaagtaat ttgatatgta
      541 ttttattggt attgtatata ctagagtaaa gactaaaaat ggtatcatta gtcttctaaa
      601 cttatttgtt aataatgagc caatgccatg ccatcgatta tttttaacct gaatagagaa
      661 aaacgcacca gatatagcca tgaataaagg catatgaaaa taataaataa tctctgtcaa
      721 tttacttaac agcttatata cagctaagga taaatggttg tttatgtatt gttgataatc
      781 tatcccacca taaccagtat aaatattata gtaggtgctg tgtcctacca caactaagat
      841 aatggctatt acttttaaaa tttcgtattc ttttattttt ttcatactaa tccttgtcat
      901 ttagagttcc tcttataatg ttttctatta tgcaaaaaga aatcagctgg gtattcacga
      961 gtaataaccg ttttctcctg agtaccatac tcaaagtgtt tatgctcaat aatacgagta
     1021 taaggaattt ctcgttctgt atagtttaca tctgcgtttc cttgatagtt ttcctcatct
     1081 agaacttcat gatcaaaacg aagactacgg tattctaact caccatgttt ataatcaaag
     1141 tattggtcaa tcattcctgt aaagacaacc ttttcagcag aagcctctaa ttcttgacga
     1201 ttggcaaaaa agttcactcc aagttctact tctacatcct tcagcatatt ttcgatgata
     1261 acattataat caccaattgg aatcccttga taggtatcgt taaaatagtt gttatcatag
     1321 gtcaggtgta ctggtaaacg gcgaataata aaggatggaa gttcagtagt tggtttgccc
     1381 cactgtttct ctgtatagtc tttgattaat ttttcgtaga tatctgtacc tacaagagaa
     1441 atctcctgtt cttccaaatt ttcaggagtt ttaccattta aaatagcacg ttgttcctca
     1501 atcttagctt gtgctcctgc tggcgttaca actccccaga gtttattgaa agtattcata
     1561 ttaaaaggaa ggttataaat ctcaccctta tagtttgcaa caggagaatt tgtgtaacgg
     1621 ttaaactctg caagctgatt tacataatca cagatctcct tatcagaagt atggaagata
     1681 tgagcaccat attgataaac ttgaattcct tcctcttcac gagtatagat attacccgcg
     1741 atatgatttc gtttttcaat aacttttact tttttccttt taaggctgct tcatgggcaa
     1801 agactgcacc aaagagacca gcaccaacaa caagataatc gtacatctta agctcctttg
     1861 gagttcatta tctgcctctt ttattagact agaaaatcat tctactactt atctcacttc
     1921 ttgtttgtaa aattcttgca atgcatcttg ccaagttgga ataacaaatc cagtagcttt
     1981 ggctttggcc aggctcatcg ttgagtttag cggacgttta gctttggctg gaaattgact
     2041 ggaatctact ggcttgactt cgacatctgt atctttcaaa atttcaactg caaaatcata
     2101 ccatgttgtg tcttttgtcg catcatttga caaatgataa taaccaaatt ccttgcggtt
     2161 ttcagctagg taggtcatga attcagccaa ggtacgagtc caagtcggac gaccgtactg
     2221 gtcatttaca actgttaaag tcttatgagt tttcgcaaga ttttgcatgg taaaaacgaa
     2281 gtttttgcca taatttccaa atacccaggc agtacggata atatagaaat tagacacatg
     2341 cttctcaact aactcttccc ccatacgctt agtacggcca tattctgtct gtggatctgg
     2401 tcggtcatca acttcccact cttgtccaac tggtttctta ccgtcaaaga catagtccgt
     2461 agaaatataa actagagttg caccatgctt ttcagatgct tttgcgacat tttttgtccc
     2521 cgtcacattg atggcgaagt ccaactcttt tccttcatcc tctgctgcat caacagcggt
     2581 gtaggctgca cagtggtaga ctaaagtcgg tttcacctct tcaaaaactt tctcaaccat
     2641 ttctgcatcg gtaatgtcca tctcagccac atctactgcc acgtattctt cattacgttc
     2701 atccaataaa tagcgaagtt ccgttcctaa ttggccattt gcccctgtaa ttaaaatcat
     2761 ctctaggcct ttctaatatg taactcttcc caatatagca ataaaaagta gctatttcct
     2821 gctttttata ctgtaataat ctcctgagtc ttagcataat tggcttcaac agcttctttt
     2881 tctgctttcc accattcttg gttatctgta taccacttga ttgttgcctt gagcccagct
     2941 tcaaagttgg taaattcagg tttccacccc aactcatcac agagcttgct tgcatcaatc
     3001 gcatagcgaa ggtcatgtcc tgcacgatca gtcacatgat cataggcatc cgcagtttgt
     3061 cccatttcct taaggataag ttccaaaact tccttattgt tcttctcacc atcagcccca
     3121 atcaagtagg tttcaccgat ttgccctttt atcaagattg tccaaactcc tgaagaatgg
     3181 tcattggtat ggatccagtc acgaacgttt ttaccttcac catagagttt tggcttgata
     3241 ccgctcaaga tattggtgat ttgtcgtgga ataaacttct caatatgctg gtaaggacca
     3301 tagttgttgg aacagttgga aatagtcgct ttgacgccaa atgagcgtac ccaagctttg
     3361 acaatcaagt ctgaagccgc cttagtcgat gagtaaggcg agcttggatt gtacttggtt
     3421 tcagccgtaa atttctcacc tggtccttcc ccatgacctg gtaaatcttc acgcagcggc
     3481 agatccccat agacttcgtc agtcgacaca tggtggaaac gaaggtcgta tttacgagcc
     3541 gcttccaaga gtgtataagt tccgatgaag ttggtatgga taaatggcga ggggtcatta
     3601 agcgaattgt cattgtggct ttctgccgca tagtgtacaa tggcatccgc ttcagctgct
     3661 aacttatcta ccaaggctgc atcagcaata tctccaacga ctaactcaac acggtcgcct
     3721 aaaatttctt caatattggc acgattacct gcataagtca gcttgtccag cactgtcaca
     3781 tacacatctg gaaagttatt gtaaacatag tggacaaagt tagaaccgat aaaaccagcc
     3841 ccacctgtca cgatgatgtt tttatattta gtcatattat ttccttattc caaatcttct
     3901 tttttcaaag gtttcacgtc cttaagtagg ggatgatgtt tgtctgcttc agatacctct
     3961 gcttcttcca gattttccca ctcaattcct aattctgggt cagagtagtt aacaaatgca
     4021 tacttaggtt tgagttcaag cgcccaatag tcattaacca gataactata agaaactgta
     4081 tcagatagga cttgaaatcc attggctacg ccacgaggga caaagattcc cttactagcg
     4141 tcaatctctg tctggtaaat attcccaaaa gtttcaccct cacgcaaatc aacccatgtt
     4201 cctagaacct tcccattatc tgcaacagag atgtacttgt cccaaggctc agcatggagg
     4261 cctcgaagaa catttttgcg agaaaagctg acgttatttt gcagtttccc ttcagcaaag
     4321 aagctttcag gaaagccaag tggcaccatc ttttccttct ggaaattttc cttaaaccaa
     4381 ccacgattgt ctccatgaac gggaatatca aactctaaca tacctgaaat ggcatcaatt
     4441 ttacgaactg caagtgtttt tccaaaaaaa ttatctgtca tctagacttc tccaatcaaa
     4501 cggagcaaat atcgtccgta ttcattcttc ttcagaggtt gtgccaactc tagtacatct
     4561 tcacgactga tatagcccat acgataggca atttcttcca agtttgcaac ttgaacattc
     4621 tgcatccgtt gaactgtttc gatatactga gaagcttcta gtaaactttc atgagtgcca
     4681 gtatccaacc aagcaaaacc acgtcccatg agttcaacag acaaatcacc acgatccaag
     4741 taagccttgt tgatatctgt aatttctaat tcgccacgtg cacttggttt aatgccttta
     4801 gcaatttcta caacatcatt atcatagaaa tacagaccag tcacagcata gttagagcga
     4861 ggatccttcg gtttttcttc tatggaaatg gcattcatgt ctgtatcaaa ctcgactaca
     4921 ccaaaacgct ctggatcctt cacttggtag ccaaaaacag tcgcaccttt ctctttctgg
     4981 gctgcctttt gaagcatttt gctcaaacca ggtccatgat agatattatc gcctaaaatt
     5041 aaagcaacac tatcgtcacc gataaatttt tcaccgatga taaaggcttg cgctagtcca
     5101 tctggactag gctgctcggc ataagaaagc ttgataccaa actcggaacc atctaaaagt
     5161 aaatccttaa aacgaggtag atcttgagga gttgagataa tcaaaatatc tttaattcca
     5221 gccaacatta atgtcgacaa aggataataa atcataggtt tatcataaac cggcatcagt
     5281 tgttttgatg cagctcgtgt aagggggtac agtcgggtac ctgagccgcc tgcaagaata
     5341 atacctttca taatggaata acctttctcc gttgtttttc aattactaaa ttcttcttct
     5401 actacgctaa gcgctcgctc aataactaca tgcatatcat aatatttata atctgctaac
     5461 cgtccacaga agataacttt atcattcttt gaagcttcct cttggtactt agcaaacata
     5521 gcattatttt tctcatcgtt gatcggatag tagggctcgt ctccacgctt ccagtcagcc
     5581 ggatattcac gcgtgataac tgtctttggc tgcgttccat attcaaaatg tttgtgttcg
     5641 atgatacgag tataagggat ctcacgctct gtatagttca ctacagcatt cccttgataa
     5701 ttttcctcgt ccaaaatttc atgctcaaag cgaaggctac ggtattctaa ctccccgtgt
     5761 ttgtagtcaa aatactggtc gatcattcct gtgaagacaa ctttttcagc tgatacttct
     5821 aactcttcac ggtgagcaaa gaaatcaaca ccaagctcaa cttcaacgtc tttaagcatg
     5881 ttttcgataa ttacattata accaccaata ggaattcctt ggtaacggtc gttaaaatag
     5941 ttattatcaa atgttagacg aactggaaga cgtttaatga taaatggagg aagttccgtt
     6001 gctgagcgtc cccattgctt ttcagtatag cccttgatca acttttcata gatatccgga
     6061 ccaatcaact tgatagcttg ttcttccaga ttttttggct caacatcctt cgtatcagcc
     6121 gtctgctcag caatcttatc tttcacttcc tgtggagttt ttgtccccca catagcatag
     6181 aaagtattca tattgaaagg aagattataa agacttcctt tgtagttcgc gacaggtgaa
     6241 ttgatatagt tgttaaattc agcgaattga ttgacatagt cccaaacttt tttattagaa
     6301 gtatggaaga tatgggcacc atatttatga acattgatac cttctacatt ctcacagtat
     6361 atgttccctc caatgtgatc gcgtttatca atcactttta ctttttttcc acgttttgta
     6421 gcttcgtgag caaaaatagc tccagacaaa ccagctccaa cgattaaata gtcgtacata
     6481 ttaaatctcc ctatttttta atagttcctg tttcaactct ttcaaattca ccaccttgaa
     6541 aagcaaaatc aaggctacat aaatcactcc acctagcaaa gcatacagag caacattaat
     6601 agcaggtgaa acatgaacaa cggacttcat agataccaag aaagcataca tgatccccga
     6661 agcaataaca atcttgatca ttgagccaat aattggtacc tctttaagat aagaccatgt
     6721 gaagtgaagt tgaatccccc ataccaaagc ttctgtcaac acagacacaa tcgctgcccc
     6781 gataaaacct agattcggta agaagatgag gttcaatccg acactgacaa tagcgggaac
     6841 agtggtcgaa atcataaatt ctttattttt attatggggg atgaggattt gaattcccat
     6901 gatattggtc catccaataa agaacattct aaacatcata attgcaattg catagcgggc
     6961 atcttggaaa tcccttccta aaaagaattg aacaaagtcg tcattcacga tcaacatccc
     7021 agcaataatt ggaaaaatga ctgaattata aattaaaaag gacatttggt acattttctt
     7081 cacagactta taatcacttt gtgatagaag actagacact cgtggcaaca taacactccc
     7141 tagtgaggtt actagagtca acaagatgtt tactagcttt aaagcctgat cataaatccc
     7201 tacatctgtt tttgaagcta aagctccaag catagttcta tccaaggtca catacaagga
     7261 aatggcgatt tgaggaagaa agagaagaat gacaggcttt agatgcttct ttgcgtggtt
     7321 aatatctata tgtggctttc caataaattc tcgagcaggc acccacatac tcaactggcc
     7381 taacagttca aatatggtta gtaaaaaaac atagagataa aggtctttag cagattttac
     7441 aaacaagaag attgagatta caccgacaag tttgactgta atatttcgca caataatctt
     7501 ccggaagtct tccaaacctt gaaataacca agaaatatct aatccctttg aaagcaaact
     7561 aaaccctaag atataggcaa ctgaatcttg catagatggc aaactcagac atagtatagc
     7621 atagagggta ccggataaaa tagtcgctcc tagctgtagg gtataaatcc cccagaaatt
     7681 ccttcgaatt tcttttcgat gccctgaaat ttccttggta ccataattag ctactcctaa
     7741 agttgccaat aagataaaat aggtgacgat tgagttgaaa taaccatagg tacccaagtc
     7801 atccgagcta aacacccgag taatgtaagg agtagtaata accggcaaaa taatcagcaa
     7861 cagttgataa gagagattat aggcgtaatt tttaagaact ttcataccat atccttatct
     7921 gcttgagttg atgaaacagc atttgctaac cctaataaca acggaagaag cataattcta
     7981 ttataatcat ataaattttg cgaagcaaag ctagttacta atacaaatag agaaagacaa
     8041 aataacagca tacctttaaa gtgtaatcta ttcttgaaac caactaaaca tccatacaaa
     8101 ataggtaata aacaaatgat aaaacctata attccgaatt gatagagcat tctaataata
     8161 cctaaatcag ataaataata cttccaacca gcaaaaatat tgccatctga gattggccca
     8221 aaacctaaaa gtttattttg attccatatt tcttgatagt atctccttcc aacaatacga
     8281 accatcgtac ctgcaccata atcgctattt gatacagaga aactatcaat aaatttttca
     8341 taatatggac tcagtagaat tgcaaaagaa gctaaactga gtgataaaaa actgagtaat
     8401 ttatttagta agttcttatt ttttaataaa taaattgcaa taaacattat actgaaacca
     8461 ataagttgtg acctagaagc ataaatcact ccctcataga acaaagtaat caacaaaagt
     8521 agactataag atactttatt ctttgaatca cttattaata acttacataa caaatatgcc
     8581 aaaacgtagc catccaaaaa aagggcttgt agacgaaccg ctccataacg tgtccaattc
     8641 acacctcttt cttcaattat acccggagcg atatttaaac cagcataatt ataactcatc
     8701 caaataaaag tacgaaataa aattgcaaga attccaaata caaaaactac ttttaaaaaa
     8761 tttaaactat actttggctt taatatctct gatattggaa agactagtag aataaaactg
     8821 aaatttctca acaaatatat tttactatta aaagacaaaa agccattaac aggtagtcct
     8881 tgtctatata aattgaacat tatcaataag aaaaataata gaactgtaat tgacgtgaaa
     8941 attgttaact ttgatattcg tctttgtagt aaatacattc ccatcaataa aaatgtcaaa
     9001 taaaccgaaa aattttggat tgtataggtt aggtctgaaa caatattaaa gaataataag
     9061 ttcataaaaa aaatgaggta gagaaaataa cgatatatat cgtgaattga aagtttaatc
     9121 attgtttcct aaacctctca tatcccttaa ttttatccat tatacgattg tataacaaac
     9181 atagtttgta ctgtctaaac ctcaacaatt ttctgaaaac aacataacga tttccaatag
     9241 aataatctag gcgattacta ttaaaagctt ttgaaaagtc attatcctca attgtagatt
     9301 tcattaattt tgatgcttca ttcaatgaat aagttccatt caaccacaat ggtccaaaat
     9361 aacgatcaat ataaacagtc atagccttca agataataaa attctctatt aagactattt
     9421 ccgatgattc agagtatcct tttaaaattt gacgtattct atctacaaat acaatctgcc
     9481 cttttaaatt tttttcattg tagtacatta aactatgagt cccattgaca tgataaaaat
     9541 ttacagggga tagaatcaca tgtcttgagg ctgtcagcaa tgtaaaatta aataaaatat
     9601 cctcagcaaa tgacagttgc gtgttaaatc tcaattgaat atcattaatt aaacttgttc
     9661 taaaaaattt cccagctatt cc
//
LOCUS       DAVKHZ010000077         9618 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000077 DAVKHZ010000000
VERSION     DAVKHZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.077"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            25..1026
                     /locus_tag="VWW99_001769"
     CDS             25..1026
                     /locus_tag="VWW99_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9539572.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1238..1467
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1633..1710
                     /locus_tag="VWW99_001770"
                     /pseudo
     CDS             1633..1710
                     /locus_tag="VWW99_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2257..3618
                     /gene="trpE"
                     /locus_tag="VWW99_001771"
     CDS             2257..3618
                     /gene="trpE"
                     /locus_tag="VWW99_001771"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9539573.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNSFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGVTDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3615..4181
                     /locus_tag="VWW99_001772"
     CDS             3615..4181
                     /locus_tag="VWW99_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9539574.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4192..5196
                     /gene="trpD"
                     /locus_tag="VWW99_001773"
     CDS             4192..5196
                     /gene="trpD"
                     /locus_tag="VWW99_001773"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9539575.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDVRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5193..5960
                     /gene="trpC"
                     /locus_tag="VWW99_001774"
     CDS             5193..5960
                     /gene="trpC"
                     /locus_tag="VWW99_001774"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9539576.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLNYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5947..6546
                     /locus_tag="VWW99_001775"
     CDS             5947..6546
                     /locus_tag="VWW99_001775"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9539577.1"
                     /translation="MTKVKICGLSTKEAAETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPVDVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6524..7747
                     /gene="trpB"
                     /locus_tag="VWW99_001776"
     CDS             6524..7747
                     /gene="trpB"
                     /locus_tag="VWW99_001776"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9539578.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7740..8516
                     /locus_tag="VWW99_001777"
     CDS             7740..8516
                     /locus_tag="VWW99_001777"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9539579.1"
                     /translation="MPKTLTEKLNAIKVTGKGIFVPYIMAGDHEKDLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(8488..9147)
                     /locus_tag="VWW99_001778"
     CDS             complement(8488..9147)
                     /locus_tag="VWW99_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9539580.1"
                     /translation="MIDIYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLH
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFQLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9217..>9618
                     /locus_tag="VWW99_001779"
     CDS             9217..>9618
                     /locus_tag="VWW99_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539581.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLDSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRVQNLYQKEGFEIVQM"
BASE COUNT         2923 a         1797 c         2314 g         2584 t
ORIGIN      
        1 gaatgttcgt aatggaggac tgaaatgaaa aatataaatg ggaaaaaggt aactatttat
       61 gatattgcac gtttatcagg tttttctcca aaaacagttt cacgagttat taatggtgga
      121 gtaaatgtta aagaagaaac ttatcaagcg attcaaaaag ttatagagga attgtcgtat
      181 attccaaatg cgtatgcaaa aaatttaact aaaaaagaag ctataaatat attgatttct
      241 gtaaaaaaaa tcgactcttt tcctttaatt tggtttcata cgctgttaga taaagtgtta
      301 cggacgtgta aagaatttgg tgttaatgct atagttgagt actttggaga ggaggataca
      361 attagtaatt caattatatc aagcacaggt agcctagtag atggtgttat tgttttttat
      421 gaaagtgtag atgatatcag aattcagtat ttaaagaaga accatatgcc ttttcttgtt
      481 ttcggtgaat ctcaaacgtc tggagtagtc tatgtatcta ataataattt tcaagctact
      541 tatgatatga tgaaagctgt aactgaagaa aaatttaaaa atatgtggtt gcttatggga
      601 ggagaatccc atgttaataa ggatcgtgaa agaggtgtgc gttcttttct gaatgataaa
      661 aattacttta tggatttaaa agtcatttat ggcttatcta caattgattc agtttattct
      721 tatgcaatgc agcacttgac tacccaaaat tatccggaca ttatttttgt ttcaggagat
      781 gaaaaggttc agggcttgat tcgtgcgtgc tatgagaaag gaattcttat tccagatgat
      841 atttctatca ttggatttga taatattcct atttctcagt attatactcc agctttgtct
      901 acaattgctc ccaattatgt taaattggct aaagaaatga tagaaggtgt tttagcaatt
      961 attaaagggg aaagtgtcac atctgttgaa gtttctccta aatttgttag gagacagagt
     1021 ttctagtatt gtttaattct tgaaaatcct agtggaaaag atggagtgtt ctaaaataag
     1081 ttctgtttag cctccattca gtttctattg agttggatcc aatttctaac aagtgggaca
     1141 tgttcatgtt cgatctaagg aagacatggt cacattcgaa tgatattttc aaaaaaatcg
     1201 taaaaaagtc ttgacaaaga aaaaacaaag tattaaaata agttcaacaa atcagaaaag
     1261 taactatttt tgatcttcag ggagcctgtg gtggttgtga ataggtggtt ggaagtagtg
     1321 aaagtgggct gattttgaaa atgaatttga aacaatgaaa attcggtgcg cacaccttac
     1381 agtgcagctt gttgttaaac aaggcagaga tgtaaaggga tagtcccttt ataattgagg
     1441 tggcaccgcg ttaccaacgc cctcacacgg aagtatattt tgtgtgtggg cttttttcta
     1501 tccgtcgttt ggtttatctt ttattagggc cttaagtcgc tttgatgaac ttgagttcta
     1561 tctacagctc cttgcctact actaaaagca aacaaaaagc tggactaata cgaaaaaagc
     1621 tagtaatact caatgaaaat caaagagcaa actaggaaac tagccgcagg ttgctcaaaa
     1681 cactgttttg aggttgtgga tagaactgac gaagtcagct caaaatactg ttttgaggtt
     1741 gtggatagaa ctgacgaagt cagctcaaaa cactgttttg aggttgcaga tagaactgac
     1801 gaagtcagta accataccta cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa
     1861 gagtataaag gtgtatcgaa ataaaaatct cctcatcaat atctctggtc gtggaggcaa
     1921 ggacctagct gcgattgcag attacctaga agctaaaaaa taatagatgg aaaaattaca
     1981 gtcttttctc tcacagagag cttgtggttg ctgaaaacaa gcagagaaag ctgtaaagtt
     2041 gggttcatct gaaacgagag aagaaaacat cattctcaag ggagtgccct ttatcgcaca
     2101 gcctgttaca ggaggtatct gagacaggaa tgagagatag gagaaatcct ataattgagg
     2161 tggcaccgcg aatttcgtcc tcacgcaagt tattttgcgt ggggattttt catacagtca
     2221 ccacggacta gaagtagaac tgaaagggaa attacaatgg aacgaatcat tcatggagat
     2281 gtcttatcac caatcttggc ttatatgcgc ttaaaggggc aacacaaggt tatcttagag
     2341 agtattccga gagacaagga aacagctcgt ttttctatct tagcctataa tcctgttttt
     2401 gagattaagt ttgaaaatgg agttctttat caaaatggtc aagtgattga tcgagatccc
     2461 ttggatttcc tttatgaagt gattcataag agtcagcacc actcagagct accttttggt
     2521 ggtggagcca ttggttttgt gggttacgat atgatttcgc tttatgaaga aattggtcaa
     2581 atccctgagg atacaattgg gacgccagac atgcatttct ttgtctatga gagctatatg
     2641 gtctttgacc acaagaagga gaagatacat gtcatagagg atgctctcta tagtgagcgc
     2701 agtcaagagg ccttggaaaa atccttgaac caagtgcttg aggaattacg cattcctgct
     2761 ccaaatgaat ttgaagactt ggatttatca ccacttgact tcaaaccgca tatcgctcca
     2821 cataagtttg aggtaatggt agaaacagct cgtgacttga ttcgtaatgg tgatatgttc
     2881 caatgtgtac tcagccagcg tttctcagca gaagttactg gaaattcatt tgacttctac
     2941 agaaatctcc gcgtgaccaa tccatccaat tacctctatt tctatgattt tggggattat
     3001 caaatcatcg gagctagtcc agaaagttta gtttctgtca aaaatggcat cgtgacaacc
     3061 aatccgattg cagggacgcg accaagaggg gttacggatg aagaagacaa gaccttggcg
     3121 actgacctcc tctctgatga gaaggaaaca gcagaacatc gaatgttggt agacttgggg
     3181 cgtaacgata ttggccgcat ctctgaaacg gccagtgtcc aagttactaa gtatatgaag
     3241 gtggagctct tccgctacgt catgcatttg accagcgtgg tcaaggggcg tttgcttgca
     3301 gaactcactg ccatggatgc cttgaaagca acgcttcctg ctggaaccgt ttctggagca
     3361 ccaaagattc gggcgatgag acgcatctat gaactggaaa cggaaaaacg gggcgtatat
     3421 gcaggagcaa tcggctactt gtctgcgacg ggtgatatgg atttggccat tgccatccga
     3481 accatgattc tcaaaaatca aagagcctat gtgcaggctg gggcagggat tgtctacgac
     3541 tctatcgccc aaaacgaata ccaagaaacc attaacaaag caaaatctat gactagaatt
     3601 ggagaactaa gaccatgatt ttattgattg acaactatga ttcttttacc tataacttgg
     3661 cccaatacat tgggaatttt gcagaagttc aggttctgag aaatgatgat tccaagctgt
     3721 atgaagaagc tgaaaaagca gatggtctgg tcttttctcc tggtcctggt tggccagttg
     3781 atgctggaaa gatggaagac atgattcgtg attttgcagg caagaagccg attctaggga
     3841 tttgtttggg ccaccaagcc attgcagaag tctttggtgg taagctaggt ttggctccaa
     3901 aagtcatgca tgggaaacag agcaatatca actttgaagc gccatctgtt ttgtatcaag
     3961 gtattgagga tggacgtgcg gtcatgcgtt atcacagtat tttgattgag gaaatgccag
     4021 aagactttga agtgacagct cgttcgactg atgaccaagc catcatgggg attcaacata
     4081 aaaacctacc gatttatggc ttccagtacc atccagagag cattggaacg ccagacggct
     4141 tgtcttctat tcggaatttt atcgagaagg ttgtaaagtg aggaaactag gatgaaagag
     4201 attattgaaa aactagcaaa atttgaaaat ttatcaggtg tggaaatgac ggatgtcatt
     4261 gagcgtatcg taactgggcg tgtaactgaa gcgcagattg cttctctcct cttagctctt
     4321 aagatgaagg gggaaacacc tgaagaacgc acagccattg cccaagtcat gagaggacat
     4381 gcccagcata ttccaactga gattcatgat gccatggaca actgtggtac aggtggggac
     4441 aagtctttca gttttaatat ttccacaact gcagcctttg tcttggctgg tggcggtatt
     4501 cacatggcca agcatggtaa ccgctcgatt tcttctaaat ctggttccgc agatgtcctc
     4561 gaagccttgg gaatcaatct tgacctcaaa ccagctgaac taggtaaggt ctttgataaa
     4621 actggaatcg tctttctctt cgctaaaaat atgcacccag ctatgaaata catcatgcca
     4681 gctcgtttgg aattgggaat tccaacgatt atgaacttga ctggtccact gattcatcca
     4741 atggctttag aaacacagct tcttggaatt agtcgtccag aactcctaga aagtacagct
     4801 caggttttga aaaatatggg tcgcaaacgt gccatcgtgg ttgctggacc agaagggttg
     4861 gatgaagctg gcttgaacgg aacaaccaag attgcacttc ttgaaaatgg cgaaatcagc
     4921 ttgtcaagct ttactccaga ggatttggga atggaaggct atgctatgga agatgttcgt
     4981 ggtgggaatg ctcaggaaaa tgcagaaatt ttgcttagcg ttctgaaaaa cgaagcaagt
     5041 ccattcttgg aaacgacagt cttgaatgct ggtcttggtt tctatgctaa tggtaagatt
     5101 gatagcatca aggaaggagt tgccttggcc cgtcaagtga ttgctagagg caaggccctt
     5161 gaaaaactca gactgttaca ggagtatcaa aaatgagtca ggaattttta gcacgaatct
     5221 tagagcagaa ggcgcgtgag gtggagcaaa tgaagctgga gcaaatccag cctctgcgcc
     5281 agacctatcg cttggcagaa tttttgaaga atcatcagga ccgcttgcag gtaatcgctg
     5341 aggtcaagaa agctagccct agtttgggag atatcaatct cgatgtggat attgtgcaac
     5401 aggcccagac ttatgaagaa aacggagcag tgatgatttc ggtgttgaca gatgaggttt
     5461 tctttaaagg gcatttgaat tatctacggg aaatttccag tcaggtagag attccgacgc
     5521 tcaacaaaga ctttatcata gatgaaaagc aaatcatccg cgctcgcaat gcaggtgcga
     5581 cagttatctt gcttattgtg gcagccttgt ccgaagaacg cctcaaggaa ctgtatgact
     5641 acgcgacaga gcttggtctg gaagtcttag tggagactca caatctagct gaactagagg
     5701 tagcccacag acttggtgct gagattatcg gggtcaacaa ccgcaacttg actacctttg
     5761 aggtcgactt gcagaccagt gtagatttag ccccttactt tgaggaaggt cgctattaca
     5821 tttctgaatc tgccattttc acagggcagg atgcggaacg actagcccca tactttaacg
     5881 gaattttggt agggacagct cttatgcagg cagaaaatgt ggcccagaga atcaaggagt
     5941 tgcagattga caaaggttaa aatttgtgga ctatcgacca aagaagcggc ggaaacagcc
     6001 gtttcagcag gagccgacta tatcggtttt gtctttgcac ctagtaaaag acaggtgact
     6061 ttagaagagg cagctgagtt ggcaaagctt attcctgtag atgtgaaaaa ggttggagta
     6121 tttgtttcac caagtcgggt agaactgctg gaagcgattg acaaagttgg cttggacttg
     6181 gttcaagttc acggtcaggt ggcagatgat ttatttgaaa atttgccttg tgccagtatt
     6241 caggctgtgc aggtagatgg aaatgggcat gtccctaatt ctcaggcaga ttatctactc
     6301 tttgatgccc ctgtggcagg aagtggccag ccctttgatt ggggtcaact ggatacgact
     6361 ggactagcac agcccttctt tatcgcaggt ggccttaatg aagataatgt agtaaaagca
     6421 attcaacatt ttactcccta tgcagtagat gtatcgagcg gagtggagac agatggacaa
     6481 aaagatcatg aaaagattag aagatttata gagagggtaa agcatggcat atcaggaacc
     6541 aaataaagat ggattttacg gaaaattcgg cggacgtttt gtcccagaaa cattgatgac
     6601 agcagttttg gagttggaga aggcctaccg tgaaagtcag gcagacccaa gtttccaaga
     6661 ggaattaaac cagcttttgc gccagtacgt aggacgtgaa actcctcttt attacgcaaa
     6721 aaacttgacc cagcatatcg gcggagccaa gatttatctc aaacgggaag accttaacca
     6781 tacaggagcc cacaagatta acaatgcctt aggacaagtt tggcttgcca aacgcatggg
     6841 taaaaagaaa attatcgcag aaacgggtgc tggtcagcac ggtgtggcaa ctgcaactgc
     6901 tgcagccctc tttaacatgg aatgtaccat ctacatgggt gaggaagatg tcaaacgcca
     6961 agccctcaat gtcttccgca tggagctttt gggagccaag gtcgaggccg tgacagatgg
     7021 ttcgcgcgtg ctcaaggatg cggtcaatgc agcccttcgt tcatgggtgg ctaatatcga
     7081 tgatacccac tatatccttg gttctgcctt gggtcctcat ccattcccag aaatcgttcg
     7141 tgacttccaa agtgttattg gtcgagaagc taaacaacag taccgtgact tgacaggtcg
     7201 agatctgcca gatgccctag tagcctgtgt tggtggtggg tcgaatgcta tcgggctctt
     7261 ccatcccttt gtagaagatg agtcagtagc tatgtatgga gctgaagcag cagggcttgg
     7321 tgtggataca gagcaccacg cagctacctt gaccaagggt cgtccaggtg tccttcacgg
     7381 ttcccttatg gatgtgctcc aagatgccca tggtcaaatt cttgaagcct tctctatctc
     7441 agcaggtttg gactatcctg gtatcggtcc agagcattct cactaccacg atattaaacg
     7501 tgccagctat gtccctgtga cagacgaaga agccttggaa ggatttcaac tcttgtctcg
     7561 tgtggaaggg attatcccag ctttggaatc tagccatgct atcgcttttg cggtgaaatt
     7621 ggccaaagaa cttggaccag aaaagtctat gattgtctgc ctatcaggtc gtggggacaa
     7681 ggatgtggtt caagtcaaag accgcttgga agcagatgca gcaaagaagg gagaagctca
     7741 tgcctaagac actaacagaa aaattgaatg ctataaaagt aactggaaaa ggaattttcg
     7801 ttccttatat catggctggt gaccacgaaa aagatttgga cggtcttgct gaaacaattc
     7861 actttttaga agatttgggt gtctctgcaa ttgaagtggg tattcccttt tcagaccctg
     7921 ttgcagatgg acctgttatc gaagaagctg gcttgcgcag tttagcccac gggacctcta
     7981 cccaggcttt agttgaaacc ttgaaaacca ttgaaacaga gattccactg gtcatcatga
     8041 cctacttcaa ccccctcttt cagtacggtg tggagaactt tgtcaaagat ctggctgata
     8101 cagctgtgaa gggcttgatt atcccagacc tgcctcatga acatgccaac tttgtagaac
     8161 catttttggc agatacagat atcgccttga ttcctttagt aagcttgacc acaggaattg
     8221 agcgccagaa agagttgatt gaaggggcgg agggattcgt ctatgccgtt gccatcaatg
     8281 gggtgacagg gaaatcaggc aattaccgtg cagatttgga caagcacttg gcgcaacttc
     8341 atcaagtggc cgacatccca gtcttgacag gttttggtgt atctagtcaa gccgatctgg
     8401 aacgcttcaa tgcggtgtca gatggtgtta tcgtcggttc aaaaatagta aaagctctcc
     8461 accaaggaga gccgattcag gattttatca gacaagcagt agcttaccaa aaataatcaa
     8521 acaagtagca agtaagagga aaggcacgaa aggaagtctt tcctttttct tttgcaggag
     8581 aaaggccagg atacccgtcg cagaagcgaa ctgaatcaag atcagtaact ccgttacgct
     8641 aaagacgaga gcacaagaag ctaaaaagag gaaatcccct gcacccatgc ggatatcgat
     8701 aaaatgagcc aaaattccaa gtatgaggaa ggagaccatg accagattcc agccagagga
     8761 agctattagg attagctgga aagtcatcca gaccagtaag ggatattcct gatggtgaaa
     8821 gtcgtagata cccaaggtca aaccagcggt gattaggacg acttgcccca aggaaagcaa
     8881 ttcccaagag taaagcagaa agaggagtcc taagcctagt tcaaagaggg cataccagac
     8941 aggatagcga actttgcagt agcgacagcg aaagcgattg aagacctgtg agagaatcgg
     9001 aatcaaatct aagggatgca agcgagtctg acaggaatcg cagtgactgg ctgaactgat
     9061 aatggattgc tctggaaaac ggtcaatgac caaaccaaga aaggaagcga gaatgctccc
     9121 gacaagaaaa aaataaatat caatcatact tatctattcg taaaaaatgg gagaagtagt
     9181 ataatggaga aacatagata agatggggag gaaaagatga caattcgttt tgaagaaaag
     9241 gtgagtatag aaaacgctca gctcgtatgc cagtggtcca actccttagg caaagtcttt
     9301 caagaacaat ggatgggacc aaggattcct tttccactga cccttcaagc cttggaagga
     9361 gtcttttcaa tctttgatga acaagagttt gtgggactta tccagaaaat caggctagaa
     9421 gacagcaatc ttcatatcgg gagatttttt atcaaccccc agaaacaggg gcaaggctta
     9481 gatagccaag ctttaaggaa atttgttagt ttggcctttg aaaatgaaga tatagatagt
     9541 atttctctaa atgtcttcga ggcaaatcaa agagttcaga atctttacca aaaagaagga
     9601 tttgaaatcg ttcaaatg
//
LOCUS       DAVKHZ010000078         9394 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000078 DAVKHZ010000000
VERSION     DAVKHZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.078"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(58..450)
                     /locus_tag="VWW99_001780"
     CDS             complement(58..450)
                     /locus_tag="VWW99_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9539582.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNSFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNITEKLDKLVLEND"
     gene            complement(749..1378)
                     /locus_tag="VWW99_001781"
     CDS             complement(749..1378)
                     /locus_tag="VWW99_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9539583.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(1423..1662)
                     /locus_tag="VWW99_001782"
     CDS             complement(1423..1662)
                     /locus_tag="VWW99_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539584.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1812..2078)
                     /locus_tag="VWW99_001783"
                     /pseudo
     CDS             complement(1812..2078)
                     /locus_tag="VWW99_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2103..2414)
                     /locus_tag="VWW99_001784"
     CDS             complement(2103..2414)
                     /locus_tag="VWW99_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9539585.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2339..2580)
                     /locus_tag="VWW99_001785"
                     /pseudo
     CDS             complement(2339..2580)
                     /locus_tag="VWW99_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2581..3213)
                     /locus_tag="VWW99_001786"
     CDS             complement(2581..3213)
                     /locus_tag="VWW99_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9539586.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLTLE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(3227..4222)
                     /locus_tag="VWW99_001787"
     CDS             complement(3227..4222)
                     /locus_tag="VWW99_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9539587.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4219..4917)
                     /locus_tag="VWW99_001788"
     CDS             complement(4219..4917)
                     /locus_tag="VWW99_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9539588.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4994..6004)
                     /locus_tag="VWW99_001789"
     CDS             complement(4994..6004)
                     /locus_tag="VWW99_001789"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9539589.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(5988..6995)
                     /locus_tag="VWW99_001790"
     CDS             complement(5988..6995)
                     /locus_tag="VWW99_001790"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9539590.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6982..7935)
                     /gene="mvaD"
                     /locus_tag="VWW99_001791"
     CDS             complement(6982..7935)
                     /gene="mvaD"
                     /locus_tag="VWW99_001791"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9539591.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7917..8795)
                     /gene="mvk"
                     /locus_tag="VWW99_001792"
     CDS             complement(7917..8795)
                     /gene="mvk"
                     /locus_tag="VWW99_001792"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9539592.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEVLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8917..9246)
                     /locus_tag="VWW99_001793"
     CDS             complement(8917..9246)
                     /locus_tag="VWW99_001793"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539593.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFCKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFHRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVR"
BASE COUNT         2785 a         2165 c         1635 g         2809 t
ORIGIN      
        1 ctaactaaaa cactcataaa gtatagacaa ttttagacta ctttggatat cttttggtta
       61 gtcattttcc aaaactaatt tatctaattt ttcagttata tttgacttca tcttctcagt
      121 aacatgagta taaacactta aaatctttgt ttcattagta taatctacac gatccattat
      181 cgctgttata ggtacttcca attcagcaag caaacttata tgcgaatatc taaaaacatg
      241 agcagtcaat acaaaatcat ctttcttaat ttcacttttc ctaaaactgt ttttcaaaaa
      301 gtaattgaaa caagttatca ttaagggaga accattattt gtaaatatat aatcaccttc
      361 ttctaaaaga agatattctt ttaaaatttc taacactcta tcactaatac caattactct
      421 atatgaagct aaacttttcg gactatccaa aaatacttcg ttattggacg attacattat
      481 agtaaaacga aataagaaca ggacaaatcg atcaggacag tcaaatcaat ttctaacaat
      541 gttttagaag tagaggtgta ctattatagt ttcaatctac tatacaagac gaaattgaag
      601 aaatctttcc attataaaac gcatattatc aaggttttta aacacctaat aatatgcgtt
      661 ttttcctatt ttaaagactt ttatccacct attatacaaa gttaagtatc catcacagtt
      721 tacaattagc cttcattacc taacccattc accatccttc aagagagtaa cctttaagaa
      781 ccgaataaat aaatggcaaa tttcgttcat ttaatttaac tgcactgtta atttgattag
      841 ctccatctcc taaagtatgc actcctacta cacggtgact agcatcataa actgtagatc
      901 cactagaccc ctctaaagta tcaacttggt aatccaagaa catgccatca tcacttaaaa
      961 cttgtttttt atctgtatac atttgatgaa ttttaaaatt atatgatgga tagcctgtga
     1021 tagtcacagt tattcctgtc aaattttttt gactagtagg aagacccaaa gtccctaatt
     1081 ttgcaccaat gggctcttct agaattaata aagccaagtc atattccctt gcatccttag
     1141 aatttaaatt tctaaattcc ttcaaataac gaacttcctt tactttgatc tttccaaata
     1201 gttcttgact tggactaaca gccggaagaa cataaatatc atccgcttct ttcccatagt
     1261 catgtctgta gtagttgtga gccgctgtaa ttaacacatt atctttaata aaattcgcac
     1321 ttcccctccc ttctacattc attccatcgg ctactccata atacttagat aaaaccatag
     1381 ctgaagtcga atacggatac tgtaaagtat tatcaatttt aatcaaatca tcattaccga
     1441 taatacttct gattgctttt ggtagtatga accatacgtt ggtgaaatct cagataatga
     1501 agaatcatta gactctggac ctttttctag tgtctcactt acctcatatt cttcacctgt
     1561 actagaaata acactcaaag cagatactgt cgataactgg ctagccaata aagtactcgc
     1621 aataattgaa atacccaatt ttttataaac agttttcttc attattgtgt cctcctaatg
     1681 taattatagc gtactattct aaatttctta atctactata gaatcaagaa atctaccacc
     1741 ttctttaaat accctccatt atcacataaa caggtaaact tttcaattaa tgactgcgct
     1801 tttcaatcac gctagaggta cttacttgct tctttgatac taagttcagc cattctttcc
     1861 ttgtttttct caataaagca tgttacccaa gtgggattcg ttttggagta gtctcgcaga
     1921 gtccagccaa tggctttatt gataaaaaat tctgtttggt tcaagttatg aaggagaatc
     1981 ttttccatta attgagtatt ggtcttctct tttcttaaca actggtggtc aatagcgaca
     2041 cgtctcagcc agatattatc tgataggctc cattttatac tcaatgaaaa tcaaagagca
     2101 aactaggaag ctagccgcag attgctcaaa acactgtttt gaggttgcag atagagctga
     2161 cgtggtttga agagattttc gaagaatatt aagattattt cttctagttc agggtgttta
     2221 tacaccaaac tccctactac tcgatctagg atatctaccg tgtcccacca ggattttgtc
     2281 acgactaact gctctagctt aggcaaatcg gtttccttta gataagactg cattgctttc
     2341 aaatagttag cagccacata ttggtatttt ctaggctcct tttcccagca agtgtctgca
     2401 aaatcccaat cgataatctt tgtttttttc gcttctggaa aatattttta tagagtttat
     2461 ttctttcagg caccgcaata cctagaaaag aaaattgatg gcgcatatag gcttccatgg
     2521 gccttgcttt ttagagtctt ttgctgcttc tagctcctca agtaaatctg ctaaactcat
     2581 ctaaaactcc tcttgcccca ccaaatggtg ctgaaaggca tagacagccg cctgagtacg
     2641 atcgctgact tcaagtttgg caagaatgtt ggacacgtgg gtcttgaccg tcttgagaga
     2701 gataaagagg tcatctgcga tgcgctgatt ttcgtagccc ttggcgatga gttggagaac
     2761 atctcgctca cgcgcagtca attcttcatg aagttccata tgattgcggt ggtattcaac
     2821 cttcttgcta acctcttgct caatggccag ctcgccagca gctaccttac tgacggcatg
     2881 aagcaattca tctgcactag aagtcttgag catatagcct ttggcaccag catctaagac
     2941 tggcatgatt ttttcattgt ccaaataaga ggtcacaatc aaaatcttgg cttcaggcca
     3001 ttctttaagg attgctaagg tcgcgtcaat cccattcatc tcaggcatga caatatccat
     3061 gacaatgaca tctggacgca gttccaaggt caagtcaatc ccttgagacc cgttggacgc
     3121 ctcacccaca acttctacat cgtcttggag gtcaaagtag cttttcaagc ccaaacggac
     3181 catttcatgg tcatctacta gtaaaatttt catctttact cctttatcat tccttatcta
     3241 acaggggaat acggatatca actgccaacc cttgcttggg agctgttaat aactgaaccg
     3301 tccctgccat atcttcaacc cgctccttga tatttcgcag tccataactc aagtcgtcta
     3361 agctccctaa ctggaaacca atcccattgt ccaccacctt cagttgcaat tcaacatctg
     3421 tctgatagag gtagacatct aggcaagatg cctgggcatg gcggagcgta ttgctaatca
     3481 actcttgcag gatacggaag atatgctcct cgattttctt aggcaatttc gtcatattct
     3541 gcttgagact aaccctaaga tcactcttgt cctcaagctc ttttaagaga atttgaatcc
     3601 cttctatcaa gctcttctgc tccagttcaa ctggtcgcaa atgcaagagc aaaacccgca
     3661 aatccttctg ggcagtttct aaaatagctg tgacactctg caactggatc tgcatctttt
     3721 ctctatccaa tttcaaagcc tgctgactga tacccgataa aatcatgtgg gccgcaaaca
     3781 actcctgact gactgtatcg tgcaaatccc gagcaattcg cttccgttcc ttctcgatga
     3841 tttcctcttc ctgagcaagg ctatgatttt cagctttttg aagagcttct gtcaaaaggt
     3901 taagtttacc tgataaggac ttgaaactgg catccaaatc tggatctgca acctgaacca
     3961 cttcttgccc tgccaataaa cgcttgagat tagcctgcat ttttcttaga gaaagctctt
     4021 cgatccctcg ccaaaacagg gctaagagac aggttatgga catgctgaaa accaacaata
     4081 aaaagacaaa tttttctgtt ttttcgacat cgtgcaaaaa gatagaccag tcaaaatcaa
     4141 gtatttccag caagctgtgg gagaaaaaaa agacaaacag gaaggaggtg agagcaataa
     4201 tgacataggc ttgttttttc atcctctaac cacctccaca tcaccaatca tagtggtcaa
     4261 gaaaatcttg acactcttgt tactcttgag atagtctttt gtttcttgat gatagtgttc
     4321 attgcggagg gctcgcttgg gctggttgaa aaaagtcaaa tccccataga gacagttaac
     4381 gctgagactg acttccacat ctacaggtac gatgattttg gtcgttccta ccatctttct
     4441 gaggataatg acattgtcat gattggttaa gatgaccctc tccagatgaa tagtgtcctt
     4501 gcccatgaag cgaaagagat tgatatcatc gaattggcaa gtctggtagc ttgaaaaatg
     4561 atgaagattt ccaaaccaac gatttttctc cttcttaacc gtcacgacct cttcaaaaac
     4621 caaattggtc tgctcttttt cctggttcat catcgggtaa agaagaaaga ggctatagat
     4681 aaccgcaaca aaaatagcta gaatcacaaa aggattgagc ataacgatga aaaagaagag
     4741 aatggttgcc gctactaaaa gaagattatt tccctcttta ccagtgtagt agcgaatcaa
     4801 aagcaaaaag aggaatagta tcagcagaaa acgcgaaaaa tgctctgata ccatcaaaat
     4861 cagagctcct gtcagaagac aggcttcgat aaataaaaag attttaaatt ttctcatagg
     4921 ttcatcctct cccttctatt ttatcacaat tcaaaaaagt cacctcagtc tgaggatgga
     4981 aaaaaggcgc tggttacgcc tttttcatct gatcctttgc ttcttttaat tttccataaa
     5041 gaagatagtc tactttttgt agatctgcta tggtggcaca gttaagggaa cacataatca
     5101 agcgtaggtc tgctttccag ccttggacaa tgccaatcac ttcttcaact gtgtaggttt
     5161 caaccaattc cagaacggtt cgtgacaatc ccacagactt agcaccaaaa accaagcact
     5221 taatcatatc cagcggattc cgaacccctc cactaaccaa gagttcgacc ttatctttcc
     5281 attcttgggc attgagaagg gcctgcatgg tagactgacc ccattgattg aggtaatcac
     5341 gctggccact acgacggttt tcgatatagg caaagctggt gccaccacga cccgataggt
     5401 ccactgtacg aacaccgaat tcataggctc tttcgattgt cttggcatcc attccaaagc
     5461 ccacttcctt gaggacaata ggaacgggaa tttgctcgct ataatctgct agatgcgatt
     5521 gccagcttct aaacttcctt tctccctcgg gcatgagtaa ttcctgcatg acattgacat
     5581 gcacttgcaa tagaacagga ttcatctctt ctacagtctg aagtcctaac tcgacaggct
     5641 tgtccaatcc aatattggtt ccaaggagga gattgggatg actagacttg acagaaaaag
     5701 aatcatccgt tggatttttg agggctgcgc tataagaacc cgttacaaat aaaataccac
     5761 aggattccgc cacctgagcc agcttttgat tgatttctct tcccttatta cttccaccag
     5821 tcatggcatt gatataaaaa ggaaagtccc actttcgacc agcaaactct gtcgaaagat
     5881 cgatttcatc cagattgtaa agaggcaagg aagaatgaat cagctccacc tcatcaaagc
     5941 tattatagga acttttctgc tcaagggcat agaggatatg ctcgtcctta cgatttgtcg
     6001 tcatgtccta tcctttcttg atataagagc tcaatcccca gatcggccca acgatttttt
     6061 aaggttttgg ttgattgcgc atcaaaactc aaggcgatgc cacagtcacc accaccagca
     6121 ccactactct tggcaacggc ctgcaaatct tgactggctt ctttcaactg tctaagcaaa
     6181 ggcgtgtaaa tatctgtact caagccttct aaaagcttgc tggctacttc tacttgctcg
     6241 ataacttttt cggctttccc ctgctccaag gcttcgacca aagaaaccac cgtttctttt
     6301 gaggaactta aaaaattttg attgatattt tgcttgattt gctggaccat gtgactcgat
     6361 acagccactt ccttggtcca tcccactaag aaatcacatt ctaaagttgg tttcacttgt
     6421 gagataaaaa atccccaatc acgctccaga actgtcgcca agttttcttc ttctaaccaa
     6481 gcagccacct tctggcgatc aaatgactgg tagagaacca aatcctctgc cacaatacag
     6541 gcaaggtcgc ccatggaacc attgtctcct cgcttgagca agacagcgct agtcagcttg
     6601 aacaagagat tctgatcaac cgaaagatta tagagagcca gtaaagcctt gacaaccaag
     6661 acaacgacgc tgccactaga acctagacca aactttttcc cttctcgttc cattttgcca
     6721 cagattttta gggaaaaagg tcttaaattc tgaccgcgaa cagcgaggaa gtctcccatc
     6781 aaagcaatcg tttcttgaat caagctgtag tcaggattag gccttaagtc cactgcgaaa
     6841 tcaaacatat ctgaatagat acggtagctg tcagaaaaag caatttcagc cctcatatag
     6901 atgggaatat cctttatcaa agctaactgc cctggctcta aaatagcata ttcacctgcc
     6961 caatagagtt ttccgcaagt tttaacagca atcatcttga ctcaaatcct ttgtttttga
     7021 cacaatcaag cgataacgct gaccgaaaat ttctgataaa tgctccaagt ctttctcctg
     7081 acagaagacc ttaacattgg gaccagcatc catggtaaag tagcaggcct ctcctttctc
     7141 acgaagctgg cgaacaaagt ccatagcctc ataagaggca tccgtcagat aagaaaaggc
     7201 tggactagca gtctttgtcg tagcatgcat agccagggca tttttctccg ttaattctcc
     7261 aatcttggca aaatcatttt ccttgagata aatcagcata tcctgatagt ccttctcaga
     7321 ctgacgaacc cagtcgtcga aagtcgtcga ggtttccaca caaagtttca tcccgtcacg
     7381 gctagagatt ggttttttct tgtcctctag caccaacata atcatagcta gtttcaagtc
     7441 tgtctctaca gggtaaattt ctccactatc cttatcccag gctcctagtg gtccataaaa
     7501 actccgagaa gaagagcctg aggcaaactt ggcttcctgc gctaactgac ttctatccaa
     7561 tccaagcttg aaataagcat tacaagcctt gaccagggcg gacaaaccac tagaacttga
     7621 ggacaggccc gctgccgtag gcatattgtt ttgagtatcg atacggacaa agccctcacc
     7681 agctggacga taacggtcaa taatcttact catcttggca tgctcgacct cattttgtag
     7741 ctgaccattg atgtaaaatt cgtcagctgt tacattggct ggtaaaggcg acaaggtcgt
     7801 ctctgtatac atattttcca aagttagaga aatactgcta gtagcaggca ccatctcttt
     7861 ttcttttttc tttccccaat atttgataat agcaatattt gcgtaggaac gtactgttac
     7921 aggctctcta tccatgtctg aacagctcct ttctcttcta atctttctgc tagttcttgt
     7981 gcgtgtgtca aattggttac caaggctatg atacaacctc ctagcccacc accgctcatc
     8041 ttggcaccca gagcaccatg gctaagagtc gtttcaacca aaaagtctgc ctcagggcta
     8101 ctgactccaa tttcttttaa atgtaaatgc gcttgactga ggatttgtcc cagtacttca
     8161 gcatcttttt gtgaaatcgc aacttctgct tgctgggtta attctcccaa ggcatgcaaa
     8221 aacggtaggg catccttgcc cttattttga accacttgga tggcttcacg agtatgacca
     8281 taaacacccg tatcggcaat caccaaatag gcggataaat ccatctcaag ttctgtaaat
     8341 cctacgttct tgataaagcg aataggttgg tcactgagac aggtcttagc atccaaacca
     8401 ctaggattca tatgggcaat catttcagct cgattgacca agatttctag tacatcatga
     8461 ggcagatcag cctgatagta gtcaaatacc gcacgaatgg ccgctatgct gatagccgct
     8521 gacgaaccca tcccccgttt ctcagggata gccgagtcaa tctcacaacg aatgcaggct
     8581 tctgtgatat ccaaatactc cagtgaggca taaaccgcca tggacaaggt atcctcctca
     8641 taaaggcgcc aaggactctc tgcaggaact accttacagg tcacctccac ctccaaaaga
     8701 ggcagggaaa tggcaggata accgtaaacg accgcatgtt cccctattaa aattatctta
     8761 ctatgtgcct gaccgacacc aacttttttt gtcatttttt ccttttacta gacgaaaaaa
     8821 cgtcttattt ttcatacaag tattaattct ttcctatcta ttttattata ttttcacaaa
     8881 aaaagcgatt gtttccattc acaatcgctt ctttcattac cgaacccatt caccattata
     8941 attgacagaa tagccatcta cagtcgtatt cactgccaag gcacctgagc tataagcgta
     9001 gtaccatctg ccattgacct ggaaccaacc tgtcgtcata gaacgatgaa agaaactcca
     9061 taccattaag taaagaggaa agtcatgagg gagcatgcgc cattgacaac ctgttttagt
     9121 gacgtacaaa gtttcattaa caagtactcg tttcggccat ttataggtgc ggtgtttgca
     9181 gaaatagggt tcaatcttcg cccattcttg atcgtttaaa tcagtatcat atgctttgcg
     9241 tgtcataact ctagtttaac atttttttgt gaatacaggt tctaaataat cgaccacgaa
     9301 aatttcttaa gtggaaaacg ccttatgaag tatgctacgg gaaacttatg cacttaattt
     9361 gacaactcaa tatgtaaaaa tatatactat atta
//
LOCUS       DAVKHZ010000079         9158 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000079 DAVKHZ010000000
VERSION     DAVKHZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.079"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(551..1399)
                     /gene="dprA"
                     /locus_tag="VWW99_001794"
     CDS             complement(551..1399)
                     /gene="dprA"
                     /locus_tag="VWW99_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9539594.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1495..2184)
                     /locus_tag="VWW99_001795"
     CDS             complement(1495..2184)
                     /locus_tag="VWW99_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9539595.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2196..3074)
                     /locus_tag="VWW99_001796"
     CDS             complement(2196..3074)
                     /locus_tag="VWW99_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9539596.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(3064..3933)
                     /gene="licA"
                     /locus_tag="VWW99_001797"
     CDS             complement(3064..3933)
                     /gene="licA"
                     /locus_tag="VWW99_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9539597.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3950..4972)
                     /locus_tag="VWW99_001798"
     CDS             complement(3950..4972)
                     /locus_tag="VWW99_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9539598.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4977..5684)
                     /locus_tag="VWW99_001799"
     CDS             complement(4977..5684)
                     /locus_tag="VWW99_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9539599.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            6020..7507
                     /gene="tacF"
                     /locus_tag="VWW99_001800"
     CDS             6020..7507
                     /gene="tacF"
                     /locus_tag="VWW99_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9539600.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7517..8320
                     /locus_tag="VWW99_001801"
     CDS             7517..8320
                     /locus_tag="VWW99_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9539601.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8322..9131
                     /locus_tag="VWW99_001802"
     CDS             8322..9131
                     /locus_tag="VWW99_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9539602.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2879 a         1855 c         1529 g         2895 t
ORIGIN      
        1 aatctctact tttgaagaat ttatttttcc ttactaacaa gatttgagat ggtaggaatc
       61 attgaacata acctagccaa catcaatcac aatcatttct cctttctcaa ttacactaaa
      121 ttatagtgta ttgaatctat aacagtgcac cttggctgct aaaatatttc tataaattaa
      181 tttgactttc ctgatagagt tgttcacatc ttatttcaat tcactatact ttcccttata
      241 ctcaacgaaa atcaaagcgc aaactaggaa gctagccaca ggttgctcaa agcactgctt
      301 tgaggttgta gataagactg acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg
      361 aagctgacgt agtttgaaga gattttcgaa gagtattata tgagaaaaat cctctactct
      421 actaatccac ttagaaaaaa cgcatattat caagtgttaa aaacttgcta atatgcgttt
      481 tattatggaa atgttgggtt cattttctct taaaattgat tttttcttag aattctagct
      541 taggtcattt ttaaaattca aattccgcaa gaacatcttg cccactggtg accaattttg
      601 ctccttcttg aatcaaatga tggcaaccgt ctgatagtcc atctaaaatg ctaccaggaa
      661 tagcaaagac atcgcgtcct tcttccattg ctcgctcaca cgtaatgaga ctacctgaac
      721 gcatcttagc ctctgctaca atcacaccac gacaaagtcc agcaatgatg cgattacggg
      781 caggaaaatg aaatttcaga ggttgttcac caggtccata ttcacttaga accagatggt
      841 cattgccgat gtagtcttgc aagcgtttat tggctttagg ataaaacaca tccagtcctg
      901 ttccaatcac tgcaatggtt tttccgccat tctgaagagc tgccatatga gctgctgtgt
      961 caatgccctt ggccagacca ctgacaataa ccagttcatt ttccaagcct tgaatgactt
     1021 tttcaactga cttagctccc tgtttgctac aagcacgact gcccacgacc gctaccttcg
     1081 ggaatttcag gaggtcaaga tttcccttgt aaaataaaag tacaggcgca tcatatattt
     1141 cactcaaatc ccaaggataa cagtcatcta aaatagagaa agatggaaat ttttgaaact
     1201 ctttcgacaa atgcgcatcg tctatctgaa aataacgttc cataaaaacg gctggattac
     1261 ggcaacctga gatatctgca atatcaccca acaaaagctc ctgatcaaca ttttcaccgt
     1321 attctagcac tttcaaaatc tgttgattgg tcaaacctga tttttttaac ttatagattt
     1381 catagtttgt gattttcata aataactcca tttctttttc tactcatcta tctattcgta
     1441 aaagttcgta aaaaatcaaa aaacatccga ctattttgtc agatgttgga atctttaatt
     1501 ttcgttttta agaatttctt ctaatttacg atagtcttgg acactatcga tctcataaat
     1561 gctattgcct tctaattctt caacatagac atctagctct ttgatattat ccttaaccat
     1621 attgtcccaa tagagatcaa caaattcacc acttacataa gccttgtcga taaagctgac
     1681 aatcttttct gcagttggag catcccagaa ggatacacca ctaaggatgc gacctgcctt
     1741 gctatcaaca ataatgtctt gaaccttgta gtcatctcca taaaccaaga accattcgtt
     1801 ggtacaatct tcacgataaa cactaaaata agtcgaacgt gtcaaatcat tgcggaacat
     1861 atttttaaag agataattgt cagcatcaat aacatagctg ttggccaatt cttcttttac
     1921 aagatagaga gagtaaaagt tattgtagtc agcgtattta tcattgaaaa cgagacgaac
     1981 accgtatttc tctttcaagt aatcgaattg ttctttaaga taaccaacaa tgatgatgat
     2041 gtcattgatt cctttttctt tgagaaactc aatttggtac tcaatcaaag gtttttgatt
     2101 aacctgaacc aaggctttag gggtattttc agtcatagga cgcaagcgag ttcccaatcc
     2161 cgctgctaag ataatggctt tcacacgaat ctcctttatt ctttaataat aatataaact
     2221 ccagcaatga cgacaagtga cgtcataatg gtcagcatat ctagcggtgc acccaagaaa
     2281 acaactgcaa acaagaccgt ccatactaca tagctcacgt tcaagcctgt agccttggct
     2341 ggttgcaagc gattgatagc gatataataa gccaagtagg aaatcatatc aaaggctgca
     2401 aaaacaatca tgagacctag caattgtcca ttggctactg cagtaaatga ctgatgagag
     2461 aagagcacaa tcacaagata ggacaagaac gaagttactt gacggattaa gagggcttcg
     2521 atttcactca attcactttc catggcaaaa gagctaagaa cactctcact tccccatgca
     2581 atagcacaaa ccaaagcaca aagaatccca atgtagaaag aattaacctg ttcaacctta
     2641 taggtctgag caataatccc tccaataatc aagacaatcc caaatacagt atttttcgaa
     2701 atcttgtgct tcaaaaagaa gaaagccaat agaactgaaa tcgcagggta aatagccgat
     2761 acagatgaag ctaaagaact tccgatatac ttaactgcat aaagattggc ctgcataccg
     2821 atagggcctg ctagcaaggc tccgatgata acactgacat tgcgaatatt taagaaaatt
     2881 gagaggcgaa ctttcccttc ttttaccaag agaaaagcta gtaagataaa gatgctcaaa
     2941 aaatcatgag ttgcagccac cacaaagggt gacaaatctg taaaaatcga aaagatataa
     3001 gcactaaccg ttagacccaa gccccagaaa atacctgaga gaaggccaaa aggaactccg
     3061 tttttacttt tcatctgaac ctccataaga agccaaacct ttaatagctc tttggtaacg
     3121 attcacacca tagtcaccaa aatcttcacc ttgctcttcc ttatagacag tccatagact
     3181 ccaaatagta tcttgtaaaa ttttataaat agcaatcttt tcatgagaaa ccggtgtttg
     3241 gtcactctca tagtgagata agaaagtttc ttcctcttgg gaagtgaatt cagactctaa
     3301 aaagagggca gccaaatccc acattggatc attcattgat gaatattccc agtcaatcaa
     3361 ataaagtcgt ccttgaggtg attcgataaa gttttcaggc accaaatcga tatgacaaga
     3421 ttttctgtca acacctaagt cagccagtct tttctctaag gagaagactg catttctaac
     3481 agattcatag ttggcataag gaatttgttc ttcaatcaag gattcgtatt ttttgatttc
     3541 ttcaaaagga gcaaattctc ctcttaattc cttagcagac gtatgaatag tttgtaatat
     3601 tggagtaatt ttgtcgaact tggtcttgat tgacgttgaa tcaagcgtaa tcgcagattc
     3661 gatatactca tttactttga taccagcttc aatatcaaaa agataatttt ttacatctaa
     3721 gcctaaatcc tttagtagtt caagattgta cttttcatct tgtcgattga taagcttttc
     3781 tgtcccttta ccaaagaatt taacaatgta ttgcttattt gttgttttgg ccaaatagtt
     3841 ttgattggtc attccaccca gttgttcaac actgaggact tcctcttctt gactaagtaa
     3901 ggaagaaatt ttttctttaa tgattttctc cacaattaac ctccagtact tatacttccc
     3961 acttaaacac tgttttaaag gctgtgttta aatcggttgc aaagacacga tgaatatctt
     4021 taatttctct tacaggttct tctagataaa ggatattttt aagacgattg gcaaatttct
     4081 tgacttccat catttggata gcattttcaa aatcaatgcg accagaacga gatgacccaa
     4141 ccaaaatcaa gcccttttct aaggcatcgc gagtattgag attgacttta tattcgctaa
     4201 ctcccatcat gagaatcgtt ccctgaggac gaatgtagcg aatcaagtca ttaatagctg
     4261 gtccagtacc atcaccacca caacattcaa aagcatggtc aaaggccaaa tcttcaggaa
     4321 tattatccgt aatatagcat tctttggcaa atgagaagag ttccaacttt tcccaatgac
     4381 gaccaataac cacaatctct gcttctggca aagtatagtt gataatattg gcaaccacaa
     4441 aagctaaact tccatctcca ataacggcga tccgctcccg cttgctatga gcaagagtca
     4501 atagacgatt catagcgtgc atgcccacac tgacaaactc tgtaatggct gcaaccgtat
     4561 cttcaatagc atcataagcc accacacgat ctttagggag agaaacaaac tctctcataa
     4621 agccatcaaa tccactagac aagaaatggg tccctgtcat gtagttttca tagaattctt
     4681 catcactctg cataggagac tgattgggaa tcatgacaac tttttgacca acctcgtagg
     4741 ttccggtcgg gtcagaaatg acggttccac atgactcgtg aatcattgcc attggaagct
     4801 ttttattcaa aatcttggga tcacgttttc cctgatagta acgctgatcc gcatgacaga
     4861 cagccatgta gttgggacgg ataaggatat gattctcttg gtcaatagcc tcttcctgat
     4921 atttgacatt gataaactta ggcttagtta gttgataaat ttgattaatc attttactag
     4981 tctttctcaa tcatactttt tgcaatcttc aaatctgtta cggttgtaat cttcagattt
     5041 gagtattcac ctttggccaa agccacatct tttcctttga tcacaaagat tttacatgca
     5101 tctgtcaaga tttccttctc ttcatcagaa agagatccat aaaggtccat gaagtccttg
     5161 caacggaatg tttgaggtgt ttgtccttga taaaggtgag cacgatttgg aatatctgta
     5221 ataaattgac cattggtact ttcaacgata gtatcaaccg cttctaccac tgtgtccact
     5281 gcgtcatgat tttgggcaag ttggatattg tcctgaatca tgcgaagtgt aataaatgga
     5341 cgaacagaat cgtgggtaac aacgatatcc tctggagtaa gcggacgata agcatcaatg
     5401 gcttcaatga tgttcttaat acttgtattg cggtcagcac caccctttgt aatgatgata
     5461 cgttccttat aaagaggaag atatttatct acaagatctt ctgcatgaga aacccagtct
     5521 ccatgaacac caactacaat tttttcaata cttggctcca agacaaattt ttcaattgta
     5581 tgaatcaaaa taggtcgatc acctagctct aaaaattgtt ttggcaagtt actgatcccc
     5641 atgcgtgtgc cagttccacc ggcaagaatt cctgcataaa tcatctattt tctcctttgt
     5701 cttactaaaa aaacttattc tctctattat accacaatct agtcctatca taaaagaaat
     5761 actggtcctc cccttctaga ataggagaat tatgcagttc tattattcaa agaaactagt
     5821 ccatttctag taatgactag gaattctgta gcaattacta ataaatggct gcgaaatttt
     5881 agagtccttc agaataatat actttcctta aagaaaactt aaaacttaag atttgaaact
     5941 tcaaagtgat tacttgactt actgcccttc atttcttatt agctaacttt atgatataat
     6001 gaaaaacgag gtaaattgaa tgaaaagtat aaaattaaat gctctatctt acatgggaat
     6061 tcgtgtcttg aatattattt ttcccatcct aactggaacc tatgtcgcgc gtgtcttgga
     6121 ccgaactgac tatggttact tcaactcagt cgacactatt ttgtcatttt tcttgccctt
     6181 tgcaacttat ggtgtctata actacggttt aagggctatc agtaatgtca aggataacaa
     6241 aaaagatctt aacagaacct tttctagtct tttttatttg tgcatcgctt gtacgatttt
     6301 gaccactgct gtctatatcc tagcctatcc tctcttcttt actgataatc caatcgtcaa
     6361 aaaggtctac cttgttatgg ggattcaact cattgcccag attttttcaa tcgaatgggt
     6421 caatgaagct ctggaaaatt acagttttct cttttacaaa actgccttca tccgtatcct
     6481 gatgctggtc tctattttct tatttgttaa aaatgaacac gatattgttg tctatacact
     6541 tgtgatgagt ttatcgacgc tgattaacta cctgattagt tatttttgga ttaaaagaga
     6601 catcaaactt gttaaaattc acctaagtga ttttaaacca ctctttctcc ctctgacagc
     6661 catgttagtc tttgccaatg ccaatatgct cttcactttt ttagatcgcc tcttcctcgt
     6721 taaaacaggg attgatgtca acgttagtta ctataccata gctcagcgaa ttgtgaccgt
     6781 tatagctggg gttgtaacag gtgcaattgg agtgagtgtg cctcgtctca gttactatct
     6841 ggggaaagga gacaaagaag cctatgtttc tctggttaat agaggtagtc gaatctttaa
     6901 cttctttatc attccactga gttttggact catggtttta ggaccaaatg ccatcctact
     6961 ttacggtagt gaaaaatata tcggaggcgg catcttgacc tctctcttcg cttttcgtac
     7021 gattatcctg gccttagata ccattcttgg ttcccaaatt ctctttacca atggctatga
     7081 aaaacgtatc acagtctata cagtctttgc tgggctactc aatttgggct tgaatagtct
     7141 cctttttttc aaccatatcg tggctcctga atactactta ctgacaacta tgctatcaga
     7201 gacttctcta cttgttttct atatcatttt catccataga aaacaactca tccacttggg
     7261 acatatcttt agctatactg ttcgatactc tctcttttca ctttcctttg tagcaattta
     7321 tttcctgatt aatttcgtgt atcctgtaga tatggtcatt aatttgccat ttttgattaa
     7381 tactggtttg attgtcttgc tatcagctat ctcttatatt agtctacttg tcttcacaaa
     7441 agatagcatt ttctatgaat ttttaaacca tgtcctagcc ttaaaaaata aatttaaaaa
     7501 atcataggag tttaaaatga aacaactaac cgttgaagat gccaaacaaa ttgaattaga
     7561 aattttggat tatattgata ctctctgtaa aaagcacaat atcaactata ttattaacta
     7621 cggtactctg attggggcgg ttcgacatga gggctttatc ccttgggacg acgatattga
     7681 tctgtccatg cctagagaag actaccaacg atttattaac atttttcaaa aggaaaaaag
     7741 caagtataag ctcctatcct tagaaactga taagaactac tttaacaact ttatcaagat
     7801 aaccgacagt acgactaaaa ttattgatac tcgaaataca aaaacctatg agtctggtat
     7861 ctttatcgat attttcccta tagatcgctt tgatgatcct aaggtcattg atacttgtta
     7921 taaactggaa agcttcaaac tgctgtcttt cagtaaacat aaaaatattg tctataagga
     7981 tagcctttta aaagattgga tacgaacagc cttctggtta ctccttcgac cggtttctcc
     8041 tcgttatttt gcaaataaaa tcgagaaaga aattcaaaaa tatagtcgtg aaaatgggca
     8101 atatatggct tttatccctt caaaatttaa ggaaaaggaa gtcttcccaa gtggtacctt
     8161 tgataaaaca atcgatttac cctttgagaa tttaagcctt cctgcacctg aaaaatttga
     8221 tactattttg acacaatttt atggagatta tatgacccta ccaccagaag aaaaacgctt
     8281 ctacagtcat gaatttcacg cttataaatt ggaggattag gatgcaatat ttagaaaaaa
     8341 aagaaattaa agaaattcaa ctagccctgc tggactatat tgatgagact tgtaagaaac
     8401 atgatattcc ttattttctc agttatggaa ccatgcttgg agccatccgc cacaaaggta
     8461 tgattccttg ggatgatgat attgatattt ccctttatcg tgaggattat gagcgtttac
     8521 tgaagattat tgaagaagaa aatcaccctc gctacaaggt tctttcctac gatacatctt
     8581 cttggtactt ccataatttc gcatcgattt tggacacttc tactgttata gaagaccatg
     8641 ttaagtacaa gcgtcatgat accagccttt tcatcgatgt cttcccaatt gatcgattta
     8701 cagacttgag cattgtcgac aagagctata agtatgtggc tcttcgtcaa ctagcttata
     8761 tcaaaaaatc acgagcagtt cacggtgata gcaaactaaa agattttctt agattatgta
     8821 gctggtacgc tctccgattt gtcaatcctc gctactttta caagaaaatt gatcaactag
     8881 tcaaaaatgc tgtaaccaac actcctcaat atgaaggagg agttgggatc ggtaaggaag
     8941 ggatgaaaga aatcttccca gttgatacct ttaaagaact gattttaact gagtttgagg
     9001 gccgtatgtt gcctgttccc aaaaaatatg accaattttt aacccagatg tatggcgatt
     9061 atatgacacc accatcaaaa gaaatgcaag agtggtatag tcatagcatt aaagcttatc
     9121 gcaaaaactg attgaggggg attatacaaa ctactaag
//
LOCUS       DAVKHZ010000080         8928 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000080 DAVKHZ010000000
VERSION     DAVKHZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.080"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            28..183
                     /locus_tag="VWW99_001803"
                     /pseudo
     CDS             28..183
                     /locus_tag="VWW99_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            383..3658
                     /gene="rexB"
                     /locus_tag="VWW99_001804"
     CDS             383..3658
                     /gene="rexB"
                     /locus_tag="VWW99_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9539603.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEVMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILTP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQTEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNHYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3655..7305
                     /gene="addA"
                     /locus_tag="VWW99_001805"
     CDS             3655..7305
                     /gene="addA"
                     /locus_tag="VWW99_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9539604.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILNQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SKSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLAHVVAISKEKNGRALTNASRKADLKPLADAYNEERKTR
                     FAKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(7267..7479)
                     /locus_tag="VWW99_001806"
     CDS             complement(7267..7479)
                     /locus_tag="VWW99_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539605.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHIYSK
                     AKLTWFEEIFEEYKLYFYNLNLVFTE"
     gene            7610..8788
                     /locus_tag="VWW99_001807"
     CDS             7610..8788
                     /locus_tag="VWW99_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539606.1"
                     /translation="MTLPVRKSLHDAVLQASKANTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2763 a         1880 c         1883 g         2402 t
ORIGIN      
        1 ttactatagt tcaatcattc tatcaagaat attgaatata taaaggcacg taatattaaa
       61 acttcttctg atatcgaatt agagtccgat gttgatggcg ataaatcaga caatctaccc
      121 gtcgacatta agatactggg acaacacatt gaagtgtatt ccgtacctaa gaaacaatca
      181 taaaactcaa agtcttgcta ccgaggcttt tttattatgt gattttcagt agccagactt
      241 attctatcta atacaagtaa agctaagtat ttaagattta gcagatgatg aagaaaagaa
      301 aggatggcct aaatatttta acagatttac gaggctaatc atggtataat atagggtgat
      361 gaaaaattcc aacgaggctg agatgaaatt actttatact gatattcgaa cttctttgac
      421 agaaattcta acaagagagg cagaagagct agttgcagct ggcaagcggg tcttctacat
      481 tgcccccaac tctctttctt ttgaaaagga acgcgccgtg ctggaatgct tgtcccagca
      541 ggcttctttt tcgattaccg tcacgcgctt tgctcaaatg gctcgttatc tggtcttgaa
      601 tgatttacca gttaaaacta ctcttgatga tatcggtctt gggttggcct tttacaaatg
      661 ccttgccgaa ctcgatccca aggacttgcg tgtttatggc gctattaagc aggatcctca
      721 attgatccag cagttaattg agctttacca tgagatgacc aaatctcaga tgagtttttt
      781 ggacttggag aatttaacag atgaggataa gagggcggat ttactcttga tttttgagaa
      841 agtaacagcc tatcttaatc aaggtcagtt agcccaggga agtcagttgt cccatttgat
      901 tgaggctatt gagaatgaca aggtaagtag tgattttaat caaatcacct tggtcattga
      961 cggctttact cgtttttctg ctgaggaaga gcgggttgtg gacttacttc acggcaaagg
     1021 tgttgagatt gttatcgggg cttatgctag taagaaagcc tataccagtc cttttagcga
     1081 gggcaatctc taccaagcca gcgtaaaatt tctccatcat ctggcttcta aataccaaac
     1141 gcctgctcag gactgttctc aaactcatga gaagatggat agttttgaca aggcctctcg
     1201 tttgttggag tcttcttatg acttttcaga actcgctttg gatgtcgatg agaaagaccg
     1261 tgaaaattta caaatctggt cttgtttgac gcaaaaggag gagttggagc tagtagcccg
     1321 tagcattcgt cagaaattac atgagaactc agacctgagc tacaagcatt ttcgtattct
     1381 cttgggggat gtagcttctt accagttatc tctcaaaacc atttttgacc agtatcagat
     1441 tcctttttat cttggtagaa gcgaagtcat ggctcatcat cccttgactc agtttgtcga
     1501 gtctatttta gctttaaaac gttaccgttt tcgtcaggag gatttgatta atcttcttag
     1561 aactgatttg tatactgacc tcagtcagtc tgatattgat gcttttgagc aatatatccg
     1621 ctatcttggt atcaatggct tgccagcctt tcagcaaacc ttcaccaaat cccaccatgg
     1681 aaaatttaat cttgagcgtt tgaatgtcct ccgcctgaga attttaacac ctcttgaaac
     1741 cctctttgcc agccgaaaac aaaaggctga aaacctccta caaaaatgga gtgtctttct
     1801 aaaagaagga gctgtgacta agcagttaca agatttgaca accactttgg aagctgtaga
     1861 acaggaaaga caaaccgaag tttggaaggc tttctgccat gttttagaac aatttgcgac
     1921 tgtttttgct ggttcacagg ttagtctgga agacttccta gccttgctcc attctggaat
     1981 gagtttgtcc caataccgta ccattccagc aacagtggac actgttctgg tgcagagtta
     2041 cgatttgatt gcaccattga ctgctgactt tgtctatgct attggactaa ctcaggacaa
     2101 tttaccaaaa atttctcaaa acaccagtct tctgacagat gaagaaaggc aaaacctaaa
     2161 ccaaacgacc gaagaaggcg ttcaattact gattgccagc agtgaaaatc tcaagaaaaa
     2221 tcactacact atgctttcct tggtcaattc tgctcgtaag cagttgttct tgtcggctcc
     2281 aagccttttt aacgaaagtg aaagtaagga atctgcctat cttcaagagt tgatccattt
     2341 tggatttagg cggagagaga agaggatgaa tcacaaagga ctgtctaagg aggatatggg
     2401 gtcctatcac agtcttttgt ctagtctggt tgcctatcac cagcagggtg agatgagcga
     2461 tactgagcaa gatttgactt ttgtcaaggt tctgtcgcgt gtcataggta aaaaactaga
     2521 tcagcaaggt ctggaaaatc cagctatccc aaccagtcca agcagcaaga ccttagccaa
     2581 ggacaccttg caagctctct atcctgccaa acaggagttt tacctgtcta cgtcgggttt
     2641 gacagagttt tatcgcaatg aatacagtta tttcctacgc tacgttttag gcttgcagga
     2701 ggaattacgt ttgcatcctg atgcccgtag tcacgggaat ttcttgcatc gtatctttga
     2761 acgcgcctta cagttgccta atgaagattc ctttgaccaa cgtctagaac aagctattca
     2821 agaaaccagt caagaacgcg aatttgaagc tatttatcaa gaaagtttgg aagcccagtt
     2881 taccaaggaa gttttgcttg atgttgcacg gacgactgga catattctcc gacacaatcc
     2941 agccatcgaa accatcaaag aagaagcaaa ttttggtgga aaagaccaag cctttattca
     3001 attagacaat ggacgcagtg tctttgtacg aggcaaggtg gaccggattg accgtttgaa
     3061 agctaatgga gcgataggag tagtagacta caaatccagt ctgactcagt tccagtttcc
     3121 tcatttcttt aatgggctca attctcagtt accaacctat cttgctgccc taaaaagaga
     3181 aggggagcag aactttttcg gcgccatgta cttggaaatg gctgaacctg tccaatctct
     3241 gatggcggta aaaagtctgg caggagcagt ggtagaagcc agcaaatcta tgaaatacca
     3301 agggctcttc ttggaaaaag aaagcagtta tttaggcgaa ttttataaca aaaacaaggc
     3361 taatcaactg acagatgagg aatttcagct cctactggac tacaatgcct atctttacaa
     3421 gaaagctgct gagaagattt tagcaggccg gttcgccatc aatccttata ctgaaaatgg
     3481 cagaagcatt gccccatacg tccagcaaca tcaggctatt acaggctttg aagccaatta
     3541 ccatctgggc caagcccgtt tcctaaaaaa gttggaccta gctgatggca agcgtctggt
     3601 cggagaaaaa ctcaagcaag cttggtttga aaaaataaga gaggagttaa atcgatgaag
     3661 cttattccct ttttaagtga ggaggagatt caaaaactgc aagaagcaga agcaaattcg
     3721 agcaaggaac aaaagaaaac tgccgagcaa atcgaagcta tctacacttc tggccagaat
     3781 atcctggtct cagcatcggc tggttctgga aagacctttg tcatggcaga gcgcattctg
     3841 aaccaattgg cgcgtggtgt cgaaatttct caactcttta tctcaacctt taccgtcaag
     3901 gctgcaactg aacttaaaga acgtttagag aaaaaaatca gcaagaaaat ccaagaaaca
     3961 gatgatgtcg agctcaaaca acacttgggt cgccagttgg cagacctacc caacgctgcc
     4021 atcggaacca tggactcttt cacacaaaaa ttccttggca aacatggtta tctgcttgat
     4081 attgcaccta atttccgtat tttacaaaac caaagcgagc aacttcttct cgaaaacgaa
     4141 gtctttcatg aggtctttga agcgcattac caaggtaaac agaaagagac ctttagtcat
     4201 ttgctgaaaa actttgctgg acgtggcaag gacgaacggg gtctgcgcca gcaggtctat
     4261 aaaatctatg acttcctcca atccaccagt aatcctcaaa agtggctgag taaatctttc
     4321 ctcaaaggtt ttgagaaagc tgattttacc agtgaaaaag aaaaactgac tgagcaaatc
     4381 aaacaagccc tttgggattt ggaaagcttt ttccgttacc atctggataa cgatgccaag
     4441 gagtttgcaa aggctgccta tttagaaaat gttcagttaa ttctggatga aattggctcc
     4501 ctaaatcagg agtccgatag tcaggcttat caggcagtgc ttgcgcatgt tgtcgccatc
     4561 tctaaggaga aaaacggtcg agctctgact aatgccagcc gtaaggctga tttgaagccc
     4621 ctggctgatg cctacaacga agagagaaag acccggtttg ctaaactagg acaattatca
     4681 gaccagataa ccattctcga ctatcaagaa cgttatcatg gagacacttg gaaactagct
     4741 aaaaccttcc aatctttcat gagcgatttt gtggaggctt atcgtcagag aaaacgacag
     4801 gaaaatgcct ttgaattcgc tgatatcagc cattacacca ttgagatttt agagaatttc
     4861 ccacaagttc gtgagtctta tcaggagcgc ttccatgaag tcatggtcga tgagtatcag
     4921 gataccaacc atattcaaga acggatgctg gaattgttgt ctaatggcca caatcgcttt
     4981 atggtgggag atatcaagca atccatctat cgtttccgtc aggcagaccc gcagattttc
     5041 aatgagaaat tccaacgcta tgcgcaaaat ccccaagaag gcaagctcat tctcctcaag
     5101 gaaaatttcc gtagtagttc agaagtgctg tcagcaacca atgatgtctt tgaacgtctc
     5161 atggaccaag aggtcggcga aatcaactat gataacatgc accagcttgt ttttgccaat
     5221 accaaactga ctcccaatcc agacaacaag gccgaatttc tcctctacga caaggacgat
     5281 acaggtgagg aagaagagag tcaaacagaa acgaaactaa caggtgaaat gcgcttagtt
     5341 atcaaggaga tccttaaact tcatcaggaa aaaggtgttg cctttaagga aattgccctt
     5401 ctgacctcta gtcgtaatcg taatgaccaa attctacttg ccctgtctga gtacgggatt
     5461 cctgtcaaaa ctgacggaga gcaaaacaat tatctccaat ccctagaagt gcaagtcatg
     5521 ctagacactc ttcgtgtcat tcacaatccc ctgcaagact acgccttggt tgcccttatg
     5581 aagtctccaa tgtttggttt tgatgaggat gagctagcac gtttgtccct tcagaaagca
     5641 gaggataaag cccacgaaaa tctctatgag aaactggtca atgcacaaaa aaaggcaagt
     5701 agtcaaaaag gcttgattca cacagctcta gctgaaaaac taaagcaatt catggatatc
     5761 ctagcttctt ggcgcttgta tgccaaaccc cactctctct atgacttgat ttggaagatt
     5821 tacaacgacc gtttttatta tgactatgtt ggggctttgc cgaatggtcc tgctaggcag
     5881 gccaatctct atgccctagc actgcgtgct gatcaatttg aaaagagcaa tttcaaaggt
     5941 ttgtcgcgtt ttattcgtat gattgaccaa gtcttagaag cccagcacga tttggcaagc
     6001 gtggccgtcg caccgccaaa agatgcagta gagctcatga ccatccacaa gagtaaaggg
     6061 ctggagtttc cttacgtctt tatcctcaat atggatcaag atttcaacaa gcaagactct
     6121 atgtcagaag tcattctcag tcgtcagaat ggtcttggtg tcaaatatat tgccaagatg
     6181 gagacagggg cagtagaaga ccactatcct aaaaccatca aactctccat tcctagtctg
     6241 acctataggc agaacgaaga ggaattacag ctagcaagct attctgagca gatgcgtttg
     6301 ctgtatgttg ctatgacgcg ggctgagaaa aagctctatc ttgtcggcaa gggttctcgt
     6361 gaaaagctgg aagccaagca atacccagca gccaaaaatg ggaaactaaa tagcaatact
     6421 agactgcaag cacggaattt ccaagattgg ctttgggcta tcagtaaagt gtttactaag
     6481 gacaagctca actttagtta tcgttttatt gacgaagatc agttgaccag agaagctatc
     6541 ggagagttgg aaaccaagag tcctctccaa gatagctccc aagcagacaa tcgtcagtca
     6601 gataccatca aagaagctct ggaaatgctg aaggaggtgg aagtttataa tactcttcac
     6661 cgcgcagcta ttgaacttcc tagtgttcaa accccaagtc aaatcaagaa attctacgaa
     6721 ccagttatgg atatggaagg tgtcgagatt gctggtcaag gtcagtcagt aggcaagaaa
     6781 atcagcttcg atttgccaga tttttcaacc aaagaaaagg taactggagc tgagattggt
     6841 agtgctactc acgaactcat gcagagaatt gacctcagcc agcaactaac ccttgctagc
     6901 ctaacagaaa cactcaaaca agttcaaact agccaagctg tcagagacaa gatcaatctt
     6961 gataaaattc ttgctttctt tgacacagta ctcggtcagg aaattcttgc taataccgac
     7021 catctctatc gcgagcaacc tttctccatg ctcaaacgag accaaaagag tcaggaagac
     7081 tttgttgtcc gtggtatcct tgatggctat ctgctttacg aaaacaaaat tgttctgttc
     7141 gactacaaga cagaccgcta tgatgaacca agtcaactcg tagaccgcta tcgtggtcag
     7201 ttagctctat acgaagaggc tttatcacga gcctattcga ttgaaaatat tgaaaaatac
     7261 ttgattttac tcggtaaaga cgaggttcaa gttgtaaaag tataacttat actcttcgaa
     7321 aatctcttca aaccacgtca gcttcgcctt gctgtatata tggttactga cttcgtcagt
     7381 tctatccaca acctcaaaac agtgttttga gctgacttcg tcagttctat ccacaacctc
     7441 aaaacagtgt tttgagctga cttcgtcagt tctatccaca acctcaaaac agtgttttga
     7501 gctgacttcg tcagttctat ctacaacctc aaaacagtgt tttgagctga ctgcggctag
     7561 ctttctagtt tgctctttga tttttattga gtattagaaa ggagacctca tgacacttcc
     7621 agttagaaaa tccctgcacg atgcagtttt acaggcttcg aaagcgaata cttgggaaca
     7681 agctaccaag gaatggaatg aagtttcctt gatttttaac ggtatcggcc gtagcaattg
     7741 tgtctgtgga aatgccatca aatacgctta tgaactcttt aacggtgtca caggccaacg
     7801 cctctttcca attggtagcg actgtgttcg tcattttcat cgattgtccc ttgaccagca
     7861 attggaagag gaagaaaaac tgctcagaaa ggttgaaaat ctaaccagaa aagctcagaa
     7921 aaaggaaaaa atcaaggtca ataaaagtga ctttgacgag cgacttctaa aatggctctg
     7981 ggaaaaaggt gttttcaaac ccaatcgtgg caatcaattt gcgcctgaga gagactacca
     8041 gcttttccta gaagtctttc agggaggaag ttggaccaag gcggaaccaa agaaaaaggc
     8101 tcggatggaa gaagtccttg aaaagtgtat caaacccttt ttacttggga aatcggatga
     8161 ccaactctac cttgtcaagc taggcaagga gaaaattgac tacgaacaag aactccgtat
     8221 ccaggcagag aaagaacgca agaagaggga taaaatcgcc aagcaatatg cagacaatct
     8281 ggttctagcc atgggacccg cagagcgtgc ttatcaagat tactttggct ttacagagac
     8341 cctgactcaa gaagaacgca agtgggaaaa aattcttttt ggtaaaaata gagcagaacg
     8401 agctatcaag gccaaacaat accaaaaaga gctggaaaaa gaccaacgaa ttgccagtca
     8461 ggatccaatt gaaagaaaac agaagcagac ttggatcctc aattcctatt ttcgtgagct
     8521 tcctgaagaa aaagctcgat tttctagact cttattagaa tatcgaaaaa gtggtgaagt
     8581 acccttttca actgaatacc tgtcagatca tctcatcgac tttttctata aaatgaaagc
     8641 ctttgaattt gaaatcgcac cagaacaagt ccgagatttt ctaaaaaaaa gcctgcagga
     8701 agaccatcgc tcatcagcac aaggaagctg gatagaagga attctcctca actgcctcaa
     8761 accattttta gaacgattag tgatatgagt aacacctacc gtgtaaattt catggtaggt
     8821 gttattgtac ccccaaataa tataaaacaa agtatgaaag cgcatctcaa ttttaagact
     8881 tttttgtaaa ttaagagtat aattaaggta tactgccttt tctataga
//
LOCUS       DAVKHZ010000081         8701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000081 DAVKHZ010000000
VERSION     DAVKHZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.081"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(242..724)
                     /gene="greA"
                     /locus_tag="VWW99_001808"
     CDS             complement(242..724)
                     /gene="greA"
                     /locus_tag="VWW99_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9539607.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(795..2450)
                     /gene="mltG"
                     /locus_tag="VWW99_001809"
     CDS             complement(795..2450)
                     /gene="mltG"
                     /locus_tag="VWW99_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9539608.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2531..3025)
                     /locus_tag="VWW99_001810"
     CDS             complement(2531..3025)
                     /locus_tag="VWW99_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539609.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(3022..3486)
                     /locus_tag="VWW99_001811"
     CDS             complement(3022..3486)
                     /locus_tag="VWW99_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539610.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3496..4830)
                     /locus_tag="VWW99_001812"
     CDS             complement(3496..4830)
                     /locus_tag="VWW99_001812"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9539611.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4841..5458)
                     /locus_tag="VWW99_001813"
     CDS             complement(4841..5458)
                     /locus_tag="VWW99_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9539612.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5506..8604)
                     /locus_tag="VWW99_001814"
     CDS             complement(5506..8604)
                     /locus_tag="VWW99_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9539613.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFAVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
BASE COUNT         2386 a         1983 c         1656 g         2676 t
ORIGIN      
        1 tgtactattt taacttcaat gcactataca tctaatactc aataaaaatc aaagagcaaa
       61 ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgtagat gaaactgacg
      121 aagtcagtaa ccatacatac ggcaaggcga cgctgacgtg gtttgaagag attttcgaag
      181 agtagcaaaa tggaaaaagg agtgagtgaa gcacatcgcc tccccactcc tttttctgtt
      241 tttaggctgt tttttcaacc ttcaagattt ttacatcata gctaccaaca ggcgtttcaa
      301 tggttgctgt atcacctgtt ttcttgccaa tcaaggcctg cccaattggg ctttcatttg
      361 aaaccttacc tgcaaaggca tccgcaccag ctgaacctac gataatataa acttcttctt
      421 cgtcctcacc aatttcttgg atggtgactg ttttaccaat cgctacttcg tcctgggcaa
      481 ctgcgtcgct attgacgatt tcagcatagc ggatttttgt ttctaagcta gagatttgtc
      541 cttcgacaaa ggcttgttca tccttagctg cttcgtactc actgttttct gaaaggtcac
      601 cgtatgaacg ggcaatctta atgcgttcta ccacttctgg tcgacgaacc aatttcaatt
      661 cttctaattc tttttcaagt ttttcctttt cctcaagggt cataggatat gttttttctg
      721 ccatttttct caactttctt ctgataatat tttctaaaga aaattatgtg aagtatcaca
      781 taattttagt ttgtttagtt taatttgctg ttgacatgtt cagcgacatt gcggtcgtgg
      841 tcttcttgat tgttagcata gtagaccttg ccttctgtga catctgctac aaagtagagg
      901 ttatcgctct tagtttgatt gatgcttgac tcaatcgcat ccagacttgg actatcgact
      961 ggaccaggca tgagacctac atttttataa acattataag gtgaatcaat gttggtatca
     1021 atcgcaacat cctcagctag actgatattt tgccccagtt ttccttgggc atacaagatt
     1081 gcaatattac tttgaagtgg catatcacga ttcaaacgat tgtagaatac acctgcaatg
     1141 agcttacgat cttctgtctt ggcaccttct ttttcgacca aggaagcaat ggtcaacaac
     1201 tcattgacag tcaagttttt agatttgata gtactatagt aaggagatag gttcttatcc
     1261 atagcagcta acatctcatc aatcaagctc tcaatagttg tgctttcctt gatagagtat
     1321 gtagctggga aaaggtatcc ttccaaacga taacgcgcac cgctgtcttt tacaggcaaa
     1381 ctttccagta aagtaggata tttcgctact gcttgactga taaacgtctc atcttgaact
     1441 ttagccaaga aagcctccgc tgtcaaagac tctttgaagt caccttgcaa ttgacccaca
     1501 gtttgagcaa tctgatccaa ggtataacct tctggaattg tcaaagtcgc aagtacaggt
     1561 tcttgcggtt catctgttcc acctttttgc aactctttga gtaagtcttc tgtactcata
     1621 ctcttttgca aattgtagta acctgctttc aagtcggtat aatttttata cttggcataa
     1681 aaactaaaaa tcagaccatg ctttaccaaa ccagcttttt caagcgtcgt accgatttct
     1741 tgaacgtttg aaccttctgg aattccaacc gtcacatatt tcttagaatt agcatcgata
     1801 ggtaataagg aatctagcac gtactggtaa ccaaagtaac cacctgctga gagcaaggca
     1861 aggaatacaa ggaatgaaat cacaaaagct ttcacatgtg acttcttctc tttcttaggc
     1921 ttaacgggtt ttgctccttc tctacggcta cgacgtgatg tatctctgat gatatccact
     1981 gtttctgtag caggcgtagg tgtttctgga cctgcttgtt ctgctttttt ctctgccgtc
     2041 gtataggaaa cagctaccct tgttggggtt tcattgtatt ctctttcaag tttcttaggt
     2101 ctaacaggac ctggacctgg tcttgatcca ctttcttccg ctggagaaga aggtacatct
     2161 tgacttggat gacttggaac accaggagtt tctctttgaa tctcatctgc tggagaagct
     2221 ggtacacctt gacttgggtg agtaggcacg gtaggagctt ttctcataat ctcctctacc
     2281 gttgacaagg aatcagccat gagttcttca gctgaaggtt catttgcagg agtgcgaact
     2341 actgcctcat cttctttcag aacttcatca tagcctttta ctttttctaa atctctcaga
     2401 atctgctctt taaagcttaa tttctcttct tctcttgact tttcactcaa aagtttttcc
     2461 tccttgttga taatccataa tattatatct taaaagtcca aaaaaagcaa tctatgatac
     2521 ttttcctaac ttattttttc atttcccaga ccatatcata ccatgtttcc cctgcaaagg
     2581 ttgactggga ctggccttcg ttgacaaatc catgtttttc ataataggcg atgagatagt
     2641 catgacaggt tagattaatg ccatctctct catcttcaag agcaacttct ttcaaggctg
     2701 tcagcaattt ctgacccagt ccgagtccct gtgcctcctt ggcaatagac agacaggtca
     2761 cagagatata accaccaggc tcatgactat agtcttttat ttcttctgta aaagactggt
     2821 cttgcagatg gcggtgcagg ccaactggtc cttcgatata acccatgatt cttccttctt
     2881 tttcagcaac cagaaaagag gtctgaattt ctctcaaatg tgcttcaaag acagaaggag
     2941 gaatggcttc ttcgaccgaa aaattttcaa attcaagttc aacaatccga tccaaatctt
     3001 ctaatcttgc ttgtctgatt ttcattgttc ctccagataa aagggattaa accaaatcat
     3061 actatagccc tggctagttg catagagcaa aatttcttct tcatcaacaa aaccgttcat
     3121 ttcaaaatag gaaagcagct catcaggact ctccaaacga atccctttgt aatccagctc
     3181 aactgccacc tctttcaagg ctgcaagaag aagtgttccc aggccctgtc tctgatggtc
     3241 agactcgatg actaaagaat gtacttttag acattgcgga ttgtctgact ggggacttga
     3301 taaaatatag cctaaaagtt gattttcatc cctagctaga agaaaggtat ccgcacactt
     3361 acggatactt tcttctaaaa tatgggaaag ttgctgcttt tcagctggaa aagacgaggt
     3421 ctgaagtgcc cctatctcag gcaaatcaaa cttgcttgcc tgaatgatct taattggaat
     3481 ttccatggga acatcctatt gaacattgct tgtcaagtta gacaagagac gctcaaatga
     3541 gtattcatag gtttggatgt ctcctgctcc cataaagacg taaacagcat tgtcatggtc
     3601 taggagtgga gaaacatttt caacagtaat cacttggtgt tttttgttga ttttattggc
     3661 taggtcttct accttaacgt caccatgatc tacttcacga gccgagccat aaatttgcgc
     3721 tagataaaca gcatctgctt ggtttaaagc atgggcaaag tcgtccaaca gggcaatggt
     3781 tcttgtaaag gtatgcggtt gaaagactgc tacaatttcc ttgcttgggt atttctgacg
     3841 agccgcatcc aaggtcgcaa taatttctgt tggatgatgg gcaaagtcat caataatcac
     3901 tgtatcattg acaattttct cagtgaaacg acgtttaaca ccggcaaatg ttttcaagtg
     3961 ctcacgcacc aagttcaaat caaatcctgc tgtgtaaaga agaccaataa cggctgtcgc
     4021 attcatgata ttgtgacgac caaaggttgg aatgtggaat tgccccaagt tttgtccacg
     4081 gaaatgaacg gtgaaggttg aaccagttgt tgaacgaaga agatcactag ctacaaagtc
     4141 attgccttca gcttcaaaac cataataata aattggtgca tcagacgtaa tcttacgcaa
     4201 ttcagcatct tcaccataga caaaaagacc cttggtaatt tgtttggcat agtcgttaaa
     4261 ggcattgaaa acatcctcga gacttgtgaa ataatctgga tggtcaaagt caatgttggt
     4321 gataatagag tattctgggt ggtaaggcat gaagtgacgc tcatattcgt cagattcaaa
     4381 gacaaaatat ttggcattag ccgaaccacg acctgtccca tctccaatca agaagctggt
     4441 atctgtaatg tgagacaaga catgagacaa catacctgtc gttgaagttt ttccatgtgc
     4501 tcctgctact cccatgctaa caaagtcacg cataaagcta cctagaaact catggtaacg
     4561 tttgtagctg ataccatttt ggtccgcata ggcaatttcg acgttgttat ctggacgaaa
     4621 ggcatttcca gcgataattt ccatatcacc gtctagattc ttttcatcaa aaggaagaat
     4681 ggtaattcct gcctgctcaa gaccgcgttg ggtaaagtag tacttttcaa catctgatcc
     4741 ctgaaccttg tgccccatct ggtgcaacat caaggccaag gcactcatcc ctgatccctt
     4801 aattccgata aaatgatatg tctttgacat gttttctccc ctattctgtc attctggtca
     4861 gattcaactc ttgggcaacc cgacgttctt gttctgtttg tttacttttt ttattgtaga
     4921 tttggctctt ctttagaaaa tcataattgt ttttctttgg agcaggtgct gacacttctt
     4981 cattcttggt agggatagaa tgaacttctt ccgccaagat ataatgagac tgggtcaatt
     5041 tttggctata tttgacaaat tcaccaggat tttccttttg gaaaggagtt gtcggttgat
     5101 tgccctgtct aactagactg ggctgagaat gacgtctcgc aaggctgaaa tcctgagtta
     5161 ggtagttagc agagcgtttc tttttcaagt ccgcacgcgc ttcttcacgc gccacctccg
     5221 catagctctt tccttctttt ttaaccccta aaggagcctt tttaggtttt tcgacttgct
     5281 tttcaatcgg ttttactggt ttttcttcag taataggagc ccattctaaa taatttttat
     5341 ctcgatactc acccttgata ttactgatca gatcagactc atcatagaga ttcatgactg
     5401 gcatttcagt caacatgacc tcgtcatctg acaccaatgg aaatcgttct tgtttcattt
     5461 tctatttcct ttcaacactt cattatagcg tattgtcttg atttttcaag tgctggcttc
     5521 agaaattccc aaaatttctc taatttctgc tagggtcaga ctaccacgtg actctgtgcc
     5581 atccaatact tgtgacacca gatgtttctt ttgttcttgg agttcctgaa ttttttcttc
     5641 aatggttccc ttggtcacca agcgatagac ctcaaccgtt tcttcctgac ccatccgatg
     5701 ggcacggcca atggcttgcg cttccaccgc aggattccac caaaggtcaa ccaagatcac
     5761 tgtatctgca cctgtcaggt tcagaccgac cccaccagcc ttgagggaaa tcagaaaggc
     5821 atctctttct ccttggttaa aggccttggt catgtcttgt ctttccttgg ctggggttga
     5881 acccgtaatt ttaaaggatg tcaggcccaa gtctggcagt tcttgttcaa ttttttccaa
     5941 cattcccttg aactgagaga aaatcaagac acggtgtccg ccgtctgcca cctgtaccag
     6001 tagatctcgg agactatcta gtttgccgct ggctccctga taatcttcca taaacagggc
     6061 aggagtgtca catatttgac gcaagcgcat caaaccagat aaaatttcca cacgacttcg
     6121 ctgaaattcc tgttctgaca cttgagccag atggtctcgc atctgttgta actgggcaag
     6181 gtaaatagcc ttttgctggt cttccagttc atttttataa accacctcaa tcaagtctgg
     6241 caattcagtc agaacttctt ctttcttgcg tcgcatcacg aaaggcttga taaactgagc
     6301 cactcgctct gctggcaatt tcataaattc tttcttgctt ggcaaaagtc cgggcatgac
     6361 gatttggaaa atagaccaca actcacccag atggttttca atcggagttc ctgacaaggc
     6421 aaagaccgac ggcaccacaa attgtctcaa ggtctgggca atcttggtct gggcattttt
     6481 catgacctga gcctcatcta agaaaaggaa gtcaaaggcc atcccctgat aaaactcact
     6541 gtcctgacgg aaggtggcat agctagtcac atagatttga tggctctcgg caagaatctc
     6601 ctcacgactt gctttcaaac catgaacaac agccacatcc aactgtgggg caaatttctg
     6661 aaactcatct gcccagttgt aaatcaaacc tgacggagcg agaatcaaaa cccgactttc
     6721 ttttgtcact tgactagtca aaaaagcaat ggtctgaagg gttttcccaa gtcccatatc
     6781 atcagccaaa atcccaccaa aaccataatg atggagcatc tgcaaccagc caattccctt
     6841 ttcctgataa tctcgcaagt cagcctgaac ctgagttgcc tgtcgaggaa agtcctctgg
     6901 atgtgtcaaa tcctgggcca gattctggaa ttcttgtgaa aaagaaacac ggtctcgccc
     6961 ttcaaagaga tgagctaaac tgtaggccaa ggatttccga gcctgcaagg tcccatcttt
     7021 taattcaaat tgccccagtt cctgtagatt ttggcgaatt ttcttggttt cttcatcgaa
     7081 aaagtaaact tgattagacg aatcaatata aaaatcctga ttggcaacca aggcctgcat
     7141 ggcttggtcg atttcctcct gggcaatatc ttgaaaatca aactggattt ccaagagacc
     7201 tcccttggag gcaatctgca cctgaggact cgctaggcta taaagctctt ctagtttatc
     7261 tgataggtca acatgcccga gtttttcaaa gactggaatg atatcatgaa aaaaatgata
     7321 gacagactcc gcttttaagg cctgacgcca agattgaaaa tcggcttcaa agcccgcagc
     7381 caaacagact tggaaaattc tttcttctaa gtccgcgtca cttgaaaatg gtaattcttc
     7441 tagctcttgt cggctagata cctgtctatt tccataatca aactgaattt ctaaacgaat
     7501 ccgattatct tcttccctgt caaagtaaaa agagggcgca aaagttttga tttgtagacg
     7561 ttctggagct gaaacggtgc ccatctggat aaaaagagtc agacaggagg ccaatttgtc
     7621 tcgatcactg ctatcaaatt gcaggtattt ctttccttgt tgacccacag gtaacgcttt
     7681 aatttccttg agaagacgca tctgctggtc tgttaaaaaa taaacctgac ctttatggaa
     7741 aagtactgct ccctgataaa agacattgac cctaggactc tcactgattt ccatttcaaa
     7801 atagtccgag tattctgtta ctgcaaaggc aaatagattg gcatcagcat gcatatcctg
     7861 aaaaagcagg gtttggtagc tatccacttg atggtcaaat tgaaaatggg gcaaggccat
     7921 cagtaaattc acaccctgct caaaaaaggt cagagggaaa aagaggtgcc gaccttggtt
     7981 ttggaaaaag aggtctgaaa ccagcccttc ctccgttagt ccgtgcaaga aagtcaaaag
     8041 ttcttggctg gcatcatcaa aggcttccca agaaagagac tcctcataaa tcttgccaat
     8101 catatacgac tttctctgct cgacaatcct taaaaaaagt ggaatatccc gaatgacata
     8161 gtatttttgg ctattgattt ggccgattct cagagtccac aagatatgat tggttcctgc
     8221 ttccacctga cccacagctg ataactcata ggcgcattct gattttggag ataaaattcg
     8281 atccaaaaac ttgccaccca aggtcacctt ggtttcaaca gcctcttttt cttcatgacc
     8341 ttcttccaga ctccacaaga tttcctgacc acgctcatca tttttcagaa aatgctctag
     8401 cgctgccaaa tgcacacagt aacccctctt ttgaaaaaaa tcacaggcac aaaaaaccaa
     8461 atcatcctct aaactatagc gcagttcttc ttctgcaacg cgagcgtaga gccgattgtt
     8521 cttttccttg atgatatcaa ccttaccagt ttcataaagg gcaacacctt cgatacgaat
     8581 tttccccgga atcaatttag ccatattttc acctttacct tatcttttta ttataccata
     8641 ttttcgccta tgaaaatagc cttctaggaa gacttttctc ctagaaggct ggatttttaa
     8701 c
//
LOCUS       DAVKHZ010000082         8582 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000082 DAVKHZ010000000
VERSION     DAVKHZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.082"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            90..493
                     /locus_tag="VWW99_001815"
                     /pseudo
     CDS             90..493
                     /locus_tag="VWW99_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            709..1290
                     /locus_tag="VWW99_001816"
     CDS             709..1290
                     /locus_tag="VWW99_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9539614.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLGVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            1287..2564
                     /locus_tag="VWW99_001817"
     CDS             1287..2564
                     /locus_tag="VWW99_001817"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9539615.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            2582..3244
                     /gene="deoC"
                     /locus_tag="VWW99_001818"
     CDS             2582..3244
                     /gene="deoC"
                     /locus_tag="VWW99_001818"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9539616.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQFAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            3231..3620
                     /locus_tag="VWW99_001819"
     CDS             3231..3620
                     /locus_tag="VWW99_001819"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9539617.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3710..4762
                     /locus_tag="VWW99_001820"
     CDS             3710..4762
                     /locus_tag="VWW99_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9539618.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4906..6441
                     /locus_tag="VWW99_001821"
     CDS             4906..6441
                     /locus_tag="VWW99_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539619.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            6434..7492
                     /locus_tag="VWW99_001822"
     CDS             6434..7492
                     /locus_tag="VWW99_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539620.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            7495..8451
                     /locus_tag="VWW99_001823"
     CDS             7495..8451
                     /locus_tag="VWW99_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539621.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2437 a         1515 c         2030 g         2600 t
ORIGIN      
        1 caatttattg atgaaatagg agatagattc tactgattta taagcttata aagggggctt
       61 tttagttcac aacttatagg aggtgtacta tgaaaatctt aaaacgttac atattggaac
      121 tctgttttat tttaagtttt gctttacctt ttataaaagg aaccaatgca gataatggta
      181 gatgctttgt ggaaacctat tacggtttta cttttttgat ggaacatgct attgtaacag
      241 ctgtctttat ttgttcgttc ttaattgctt tcttactaaa aaacgatgga cgaaatggat
      301 tgctgcgggt agttattgct ttttagtcct acggattgcg acagagggtt atttcttcca
      361 catgtcacta gaagatttga tacgactttg gacaagtttg gaaatcctga cacaaacgta
      421 tcagttgggc ttttatctaa acattttgtt aggaattttg ttgataataa agtattttaa
      481 ggttaagcaa tagaaataat aatctgcctg attatagatg ataaactttt tgtttttatc
      541 tggactgtga tatttatagt tctaaatggt ttattattta taagtgttga aagagattga
      601 agaaaatggc ttcgtgactt gctttcttcg gtctttttcg ttttattatc aatttgtaaa
      661 cgatttacaa tttgagtcaa attatggtaa aatagtgtca tgagtaaaat gtactatgca
      721 gaaaatcctg acgctgctca cgacattcat gagttgagag tggacttgtt gggagaaaaa
      781 atgacttttt tgacagatgc gggtgttttt agcaagaaaa tggttgactt tggaagtcaa
      841 ctcttgctca agtgtctggg tgtcaaccaa ggagagacag tcctagatgt aggctgtggt
      901 tatggtccat taggtttgtc cttggtcaag gtttacggag ttcaggcgac catggttgat
      961 attaacactc gtgccctgga tttagctcag agaaatgctg aaaaaaataa tgcaaaagcg
     1021 acgatatttc aatctaatat ttatgcaaga gttcaagggc gatttgacca tgtcatttcc
     1081 aatcctccta tccgtgcggg caagcaagtc gttcatgaaa tcatcgagaa aagtaaagat
     1141 ttcttggaaa ttggtggaga tttgaccatc gtcatccaga aaaaacaagg agctccaagt
     1201 gccaagtcta agatggaaga tgtgtttggc aattgtgaaa tccttaaaaa agacaaggga
     1261 tactatatcc ttagaagtgt gaaagaatga gagcagttga tttaatccaa aaaaaacgag
     1321 acggtcaaga actgacttca agtgaaattg aatggctgat agaaggctat gtatcaggaa
     1381 ctgttcctga ttatcagatg tctgcctttg ctatggctgt ttattttaaa ggaatgacga
     1441 ctcgagaaat ctctgattta acgatgaata tggttaagac tgggcaagag tttgacctgt
     1501 cagccattga tggtgtgaaa gttgacaagc attctactgg tggtgtcggc gataaggtaa
     1561 ccttgatttt agctcctctt gttgctagct tcggtgtgcc tgtagcaaaa atgagtggtc
     1621 gtggtcttgg ccatacaggc ggaacaattg ataaattgga gtccattaag ggctatcaag
     1681 tagaacgtag tcaagaggat tttattcgtc aggttcagga tattggagta tctgtcattg
     1741 ggcaatcaga ccagctggtc aaagcagata agcttctcta tgcccttcgt gatgtgaccg
     1801 caactgtcga cacgattcct ttgattgcga gttcggtgat gagcaagaaa attgcggcag
     1861 gggcggatgc tattttgcta gacgtgactg tcggtgaggg tgccttcatg aagacggttg
     1921 atgaggcgcg tgagttggct caaactatgg tagatcttgg taaggttgtt ggacggaaga
     1981 cagtagcagt gattacggat atgagccagc ctttgggacg agcgattgga aatcgtctgg
     2041 aaatccttga agcattggag attttgcaag gtcaaggacg tcaggatatt acccacttta
     2101 tttgtgaatt ggctcaaatt atgcttggtt tggcaaatgt taacaaaact gttgaagaag
     2161 ttcaccaaca tcttgagaat ggtcaagcac tggctaagtt tgaggaaatg gtgcaagccc
     2221 aaggtggaga cttggaagac ctctatcgtc ctgtaaatgt agctcatgtg gtggaaatcc
     2281 ctgctcagga aacgggtgtc atttcagctc ttccagctat ggactttggt ctttatgcca
     2341 tgaggctggg tgctggtcgt gcagttaagt ctgatgcctt ggactatgaa acaggaattg
     2401 tttttgaaaa gaagattgga gactccgttc aaaagggtga aattgttgca aaagtataca
     2461 caaatgaaaa aattccacct caactagtta cagatttcca aaaatgtgtt aaaataagtg
     2521 atgaagtgaa aaaaatccgt gaaattgtag aaatcatctc ctaaatggag ggaaaaccga
     2581 aatgaaatta aataaatata tagatcatac gcttttaaaa caagatgcaa agaaaaaaca
     2641 aattgatagt ttgttgtctg aggctagaga gtatgacttt gccagtgttt gcgttaatcc
     2701 gacctgggtt gaacatgcta aaaaaggact tgaaggcaca gatgttaagg tttgcacagt
     2761 agtaggtttc cctttgggag caacaacttc agccgtgaaa gcatttgaga caaaagaagc
     2821 tatccaaaat ggtgcagatg agattgatat ggtgatcaat gttggagctc tcaaatcagg
     2881 taatttagcc ttggttgagt cagatattcg cgcagtagtg gaagcaagtg gtgataagtt
     2941 agtgaaagtc attattgaag cttgccttct gacagaccaa gaaaaagttg ttgtttgcca
     3001 atttgcccaa aaagctgggg ctgactttgt taaaacatct actggttttt caactggtgg
     3061 tgctacgata gcagatgtta cattaatgcg tgaaacagtt ggatctgata tgggagtcaa
     3121 ggctgctggt ggagctcgtt cttatgcaga tgctcttgcc tttgtcgaag caggtgcgac
     3181 ccgtatcgga acgtcagctg gggtagctat tttaaaagga gaattggcag atggcgacta
     3241 ctgagttgat tgaactggca attgaaacca gcaaacatgc atatgtcccc tattctcact
     3301 ttcctatcgg agcagtttta gtagccaaag acggaagtgt ttacacggga gtgaatatcg
     3361 aaaatgctag ctatcctttg accaattgcg gtgagagaac agctattttt aaggctatat
     3421 ctgaaggcca aagagaattt tcagaattga ttgtctatgg tcaaactgaa aaaccgatct
     3481 cgccatgtgg tgcttgtcgc caagtgatgg ctgaattttt tgaacaagat ctaaaagtga
     3541 ccttagtcgc aaaagataaa tcgacggtcg agatgacggt cggggaatta cttccatact
     3601 cttttacaga cttaaactag tctgagtcgc tctctgagtg gcacggtcct tgtggccaac
     3661 caatccatac ttgcaacatc gttgcacatc ttatttagga ggttcagtaa tgaacaagaa
     3721 acaatggcta ggtcttggcc tagttgcagt ggcagcagtt ggacttgctg catgtggtaa
     3781 ccgctcttct cgtaacgcag cttcatcttc tgatgtgaag acaaaagcag caatcgtcac
     3841 tgatactggt ggtgttgatg acaaatcatt caaccaatca gcttgggaag gtttgcaggc
     3901 ttggggtaaa gaacacaatc tttcaaaaga taacggtttc acttacttcc aatcaacaag
     3961 tgaagctgac tacgctaaca acttgcaaca agcggctgga agttacaacc taatcttcgg
     4021 tgttggtttt gcccttcata atgcagttaa agatgcagca aaagaacaca ctgacttgaa
     4081 ctatgtcttg attgatgatg tgattaaaga ccaaaagaat gttgcgagcg taactttcgc
     4141 tgataatgag tcaggttacc ttgcaggtgt ggctgcagca aaaacaacta agacaaaaca
     4201 agttggtttt gtaggtggta tcgaatctga agttatctct cgttttgaag caggattcaa
     4261 ggctggtgtt gcgtcagtag acccatctat caaagtccaa gttgactacg ctggttcatt
     4321 tggtgatgcg gctaaaggta aaacaattgc agccgcacaa tacgcagccg gtgcagatat
     4381 tgtttaccaa gtagctggtg gtacaggtgc aggtgtcttt gcagaggcaa aatctctcaa
     4441 cgaaagccgt cctgaaaatg aaaaagtttg ggttatcggt gttgatcgtg accaagaagc
     4501 agaaggtaaa tacacttcta aagatggcaa agaatcaaac tttgttcttg tatctacttt
     4561 gaaacaagtt ggtacaactg taaaagatat ttctaacaag gcagaaaaag gagaattccc
     4621 tggcggtcaa gtgatcgttt actcattgaa ggataaaggg gttgacttgg cagtaacaaa
     4681 cctttcagaa gaaggtaaaa aagctgtcga agatgcaaaa gctaaaatcc ttgatggaag
     4741 cgtaaaagtt cctgaaaaat aatggatgga agtcatttct taggaagcgg ccaagggccg
     4801 cttttttaag agttgagaca aagtcatttt gtctcagtaa agcttgctac tagacaaact
     4861 agcaagtttt attgaaataa aataagactc tgaaaggaag agcacatggc acacgaaaat
     4921 gtcattgaga tgcgtgatat taccaaggtg tttggtggat ttgttgccaa cgacaaaatc
     4981 aacttgcacc tacgaaaagg tgaaattcat gcacttttag gagaaaatgg ggctggtaag
     5041 tccacgctaa tgaacatgtt agcaggcctt cttgaaccaa ctagtggtga aatcgcggtc
     5101 aacggtcaag ttgtcaatct cgactcccca tctaaagcag ctagcttggg aatcgggatg
     5161 gttcaccagc actttatgtt ggttgaagcc ttcacagtgg ctgaaaacat cattttaggt
     5221 agtgaattga ctaaaaatgg tgtgctagat atcgctggag ctagcaaaga aatcaaggct
     5281 ctttctgaac gttatggctt agctgttgac ccttctgcca aggtagcaga tatctcagtt
     5341 ggagcccaac aacgtgtaga aattttaaaa acactttatc ggggggctga tatccttatc
     5401 tttgacgaac caacggctgt tttgactcca tcagaaattg atgagttgat ggctattatg
     5461 aaaaatcttg tcaaagaagg aaaatcaatt atcttgatta cccacaaatt ggatgaaatt
     5521 cgcgcagttt ctgaccgtgt tacagttatc cgtcgtggga aatcaattga aaccgttgaa
     5581 attgcagggg ctaccaatgc tgatttggcg gaaatgatgg taggacgttc tgtttccttt
     5641 aaaacagaga agcaagcctc taaaccaaaa gaagtggttt tgtctatcaa agatttggtg
     5701 gtcaatgaaa accgtggtgt tccagctgtt aaaaatctgt ccttggatgt tcgtgctgga
     5761 gagattgttg gtattgcggg gattgatgga aatggtcagt ctgaactgat tcaagccatt
     5821 acaggtcttc gtaaggttga atctggtagc attgagctaa aaggggattc aattgtaggc
     5881 ttgcacccac gtcagattac agaactaagt gttgggcacg ttccagaaga ccgtcaccgt
     5941 gatggcttga ttttggaaat gatgatatct gaaaatattg cccttcaaac ctactataaa
     6001 gaaccacata gtaaaaatgg aattttgaat tattcaaata ttacttctta tgctaaaaag
     6061 ctgatggaag agtttgatgt tcgcgctgcc agtgaattag ttcctgcagc tgcactctca
     6121 ggaggaaatc aacaaaaagc aattattgct cgtgaaattg atcgagatcc tgatctcctt
     6181 atcgttagcc agccaactcg tgggttggat gtcggtgcca ttgagtatat ccacaaacgc
     6241 ttgattgaag agcgtgataa tggcaaggct gtccttgttg tcagctttga attggatgag
     6301 attttaaacg tctcagaccg tattgccgtt atccacgatg gtaagattca aggtattgta
     6361 tcaccagaaa caaccaataa acaagaactt ggtgtcttga tggctggtgg aaacttggga
     6421 aaggagaaga gtgatgttta aaaaattaca acaaatttcg gttcccttga tttctgtatt
     6481 tctaggaatt ttactcggag ccattgtcat gtggatcttc ggttatgatg ctatttgggg
     6541 ctacgaagaa ttgttctata cagcctttgg cagtctgcgt gggattggag aaatcttccg
     6601 tgctatgggt cctctggtct tgattggtct tggttttgcg gttgccagtc gagctggttt
     6661 ctttaacgtc ggacttcctg gtcaggcttt ggcaggttgg attctcagtg gttggtttgc
     6721 gctgtcgcat ccagatatgc cccgtccctt gatgattcta gcaaccatcg tgattgcctt
     6781 gattgctggt gggattgtcg gagcgattcc aggtattctt agggcctatc tagggacgtc
     6841 agaggttatt gtaaccatca tgatgaacta cattgtcttg tatgtaggga atgcctttat
     6901 ccatgctttc cctaaagact tcatgcaaag tacagattcg accattcgtg ttggggctaa
     6961 tgcaacctat cagacacctt ggttggctga gttgactggt aactcacgga tgaatattgg
     7021 tattttcttt gccatcattg ctgttgcagt tatttggttc atgctcaaga aaacaactct
     7081 tggttttgaa atccgtgcag ttggtcttaa tccacatgct tcagaatatg ctggtatttc
     7141 tgccaagcgg actattatcc tatctatgat tatttcaggt gccttggcag gtcttggtgg
     7201 agctgttgaa ggtttgggaa ccttccagaa cgtctatgtt caaggttcgt cattagctat
     7261 cggatttaac ggaatggcgg ttagtttgct tgcggccaac tcaccaattg gtatactctt
     7321 tgcagccttc ctatttggcg ttctccaagt tggggctcct ggtatgaatg cggcgcaggt
     7381 accatctgag cttgtcagca ttgtaacagc gtctattatc ttctttgtca gtgttcatta
     7441 ccttatcgaa cgctttgtta aaccgaaaaa acaagttaaa ggaggtaagt aaagatgtct
     7501 attataacct tgctccccct cttggtgtct tctatgctga tttactcagc acctctcatc
     7561 tttacaagta tcggtggtgt tttctctgaa cgtggtggtg tggtaaacgt tggccttgaa
     7621 ggaattatgg ttatgggtgc cttttctgga gttgtcttta accttgaatt tgcagaacaa
     7681 tttggagcag caactccatg gctatccttg cttgtagcag gattggttgg tagtgttttc
     7741 tctatcatcc acgcagcagc gacggttcat ttccgtgcag accatgttgt cagcggtacg
     7801 gtattgaact tgatggcgcc tgccttggct gttttcttag ttaaagttct ttataacaaa
     7861 ggacaaaccg acaacctaag tcaaactttt ggacgctttg atttcccagt cttggcaaat
     7921 atcccagtga ttggtgatat cttcttcaag tcaactagtc tacttggtta tctggcgatt
     7981 gccttctcat tccttgcttg gtttattctc tttaagactc aatttggtct tcgtctccgc
     8041 tctgtcggtg aacatcctca agcagcggat accttgggaa tcaacgtcta caagatgaga
     8101 tatttagggg ttattatttc aggttttcta ggtggaattg gcggagcgat ttatgcgcaa
     8161 tcaatctcag ttaacttctc agtgacaact attgttggac ctggatttat cgcccttgct
     8221 gcgatgatct ttgggaaatg gaatccaatc ggtgctatgc tttctagtct cttctttgga
     8281 ctttcacaaa gtttggctgt tatcggttct caattgccgt ttctacaagg agtgccagcg
     8341 gtttatcttc aaattgcacc ttatgttttg acaattcttg tcttagcagc cttctttgga
     8401 aaagcagtcg caccaaaagc agatggtatc aactacatca aatcaaaata agcatacaaa
     8461 aaaacgtcag tccaatgact gaactgcccc ccaaaagtta gacagaaaaa atctaacttt
     8521 tggggtgttt ttattatgaa attaagttat gatgataaag ttcagatcta tgaactaaga
     8581 aa
//
LOCUS       DAVKHZ010000083         8426 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000083 DAVKHZ010000000
VERSION     DAVKHZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.083"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..1676
                     /locus_tag="VWW99_001824"
     CDS             <1..1676
                     /locus_tag="VWW99_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9539622.1"
                     /translation="KEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGE
                     YEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQD
                     YGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNGGF
                     APYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKN
                     PLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVS
                     DEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGT
                     KYKDFYYYGYKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKIN
                     MKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMY
                     DKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALIS
                     EIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            1944..2510
                     /locus_tag="VWW99_001825"
     CDS             1944..2510
                     /locus_tag="VWW99_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539623.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            2831..3019
                     /locus_tag="VWW99_001826"
     CDS             2831..3019
                     /locus_tag="VWW99_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9539624.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3081..3839
                     /locus_tag="VWW99_001827"
     CDS             3081..3839
                     /locus_tag="VWW99_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9539625.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            3897..4751
                     /locus_tag="VWW99_001828"
     CDS             3897..4751
                     /locus_tag="VWW99_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9539626.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            4863..5747
                     /locus_tag="VWW99_001829"
     CDS             4863..5747
                     /locus_tag="VWW99_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9539627.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDDYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            5910..6683
                     /locus_tag="VWW99_001830"
     CDS             5910..6683
                     /locus_tag="VWW99_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9539628.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESASNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNYIGSQSLQ"
     gene            6750..7571
                     /locus_tag="VWW99_001831"
     CDS             6750..7571
                     /locus_tag="VWW99_001831"
                     /inference="COORDINATES: protein motif:HMM:NF012356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539629.1"
                     /translation="MTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYW
                     IEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAV
                     MNYPLSDSIKDYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTA
                     NEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFM
                     KRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEK"
     gene            7924..8379
                     /locus_tag="VWW99_001832"
     CDS             7924..8379
                     /locus_tag="VWW99_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539630.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT         2932 a         1187 c         1799 g         2508 t
ORIGIN      
        1 caaaagaaaa aataaaagta tacctctata cacgagtatc tacgtcaata cagatagagg
       61 ggtattcgtt agaggcacaa aaatcacgaa tgaaagcttt tgctatttac aatgattatg
      121 aaattgttgg agaatatgaa gatgccggga agtctggcaa gtctattgaa ggtagaatac
      181 agttcaatcg aatgatggaa gatataaaat ctggaaaaga tggagtatct tttgttcttg
      241 tgtttaagtt atcaagattt gcaagaaatg ccgctgatgt tctttctaca cttcagataa
      301 tgcaggatta tggagttaat cttatctgcg ttgaagatgg cattgattca tctaaagatg
      361 ctgggaaact aatgatttct gttttatcag ctgtggctga aatagaaaga gaaaacatcc
      421 gtattcaaac aatggaaggt cgcattcaaa aagcaaggga aggaaaatgg aatggtggct
      481 ttgctccgta tggatataaa cttgaagacg gcaagctgtt tataaatgag gaagaggcag
      541 ttgcaataag aacgattttc gaccagtatg taaatactac gataggagcc aatgggatat
      601 ctaaatactt agagaatcat ggaattagaa aaatcccaag acagaatggt aagaatcctt
      661 tgtttgatgc aggtcttata agaaagatat taaagaatcc tgtatataat gggaaaatag
      721 catttggaag aagaacttta gaaaaagttc atggtacaag aaatgaatat aagcaggttg
      781 aacaagatga atatctaata tctgaaggga tacatgaagc tatagtttcc gatgaagttt
      841 ggcaagctgc tcaggttaag ctaaaatctc aagcaaagaa atatgagcat gtgaataaag
      901 gaaaagatac acgcacacac ttgctttctg gaattgtaaa atgcccgata tgtggagtgg
      961 gaatgtttgg gaacaagtgt atcaagaaaa agaaagatgg cacaaagtat aaagattttt
     1021 attactatgg ttataaacat aggcagatga taagaggtca taagtgtaca ttcagtaagc
     1081 aaattagaga agaattgtta gatgatgctg ttgcagaggt gattgtcaag atagtaagta
     1141 atccgaaatt tgcttctatg atgcaagaaa aaatcaacat gaaggtggat acctctgaaa
     1201 tagaaaaaga aatagataat taccaaaagg aattgagaaa gagtcattct acaaagttta
     1261 agctaattga ggaaatagat aatttagatg ttgaagataa gcattataag agaaggaaac
     1321 aggatttaga tgatagacta tatcgtatgt atgacaaaat agatgaatta gaatcatcac
     1381 taattgatgc gaaagcaaag aaacagacta ttgaagctga aaagcttaca ggagataaca
     1441 tatataaggt tctgatctat tttgataagc tctataaagt catgaatgat gtagagcgta
     1501 ggcagttaat ttcagctttg atttctgaaa ttcaagttta tgaagaaaaa caatctaacg
     1561 gacaatggct aaaatccatt acttttaagc taccaattat agaagaaaat ctcaacatag
     1621 atttggacaa tgatgaacaa gttgaatgtg taagtttatt agagaaacgt agttaatcct
     1681 caaaaggttc cccaaacttt ttgagtccca ctgacgcatc acgctgaggc ggtatcactg
     1741 cttgtacgag ttgaagcatc agcgaagtaa aagtagatgt tcagccaatg cgacgaggtg
     1801 agaccgaagg tcgataaggt gtgttctggg tataggactc gtaaagtaag taggaaatat
     1861 gatgagatga tacagagagc ctgaattggt caggtgacgt gcttatttac attagcctga
     1921 taatgaaaat ggagagatta tcgatggtta tattggaatt gtaccaaaat gattattcta
     1981 aagatttagt agcatttgat tccatagaag atgggaaagc ctttgtggct caaatcccgg
     2041 gatacactct ggaaacagaa gatggttttg aagtggagta ttttaatcct aaaaacatac
     2101 cggattatat ggaaattatt tttaacggaa atattgttcc tttatctaaa tttatgttcg
     2161 atcctgagga aaatgtaaac attatttgga aagagatttc aaatttatcc ctcaaaaatg
     2221 atagagtgat cgaaggatat tcaaaaattg atgcatatgt cgttaacaac catgaagtta
     2281 aagcctatgt cgaaacaaga gaaacgaact atcgtaaagc aaaagacttc ttagaaagcc
     2341 gcggatatga aatcgacaga agctttttcg gaagtgaaga cggtgaagcg attctttaca
     2401 gaaaaaaagg gatcgaagtt tggcatttct tgtgtcactt agacccaatg tttgtagaga
     2461 ttgaagatgt agaaggatat gttaaagaag aagttggaga gattcaatag tcaaccaaag
     2521 ttccttgtga gtatatacct ttgctagtaa aagagtcaaa acgttataac gaagggggga
     2581 agaaaaccct tgagtgtaag tctttttctt gtttagaggg aaagtattag agtagccgct
     2641 cataatttat actagatttg ataactgatt tgagacagat tttatttcta taaatattgt
     2701 gaaaattttt taataccaaa gatttaaagt tactgtgtta aaaatcctct ccacttttaa
     2761 actaatgtat tataaaagcc agttgacaca agtgttacac ctgtgataga attaacttga
     2821 ggtgatgaaa atggcaaccg ataaaaatag aataatgatt agcttggatg ataaaaatct
     2881 agagaaactt gaaaatttag tagaagatgc tagagataga aggggaatgc gactaacaaa
     2941 gtcccaggtt attgaattac tcctaaatac cgttgattat ttcgatgata ttatgggggc
     3001 tatttactca aaaaaataag agcaaaattg ctattatttt ttacaaattg gtgtaacact
     3061 agtgatatag gagtgatgaa atggttgttt atattagaca aagtaaatta ccaagtgagg
     3121 tttcaattaa taaatacaat gctcaagtag gtgcctactt acaaggagaa gaagctgttt
     3181 tatatcaatc tttttctgaa ataaaagagt taacaagtga agatatagtt gtagattata
     3241 taatggagac tagagcatta ctaaaaatga tgggcttaaa cgttccggtt catgactatc
     3301 ctattgagct taaagagttt tatggtcgaa agatttatgc tggtatttta ggagagattg
     3361 tgaatatacc tgataattgg ggaaaattta ttaagcctaa agctggttca aaagtcttta
     3421 ctggaagagt tgttaatgga acccatgatt taataggtat tggtctacct ttcgactatc
     3481 ctatatggat tagtgaggtt gtagaattca tagctgaatg gcgttgtttt gtgttagatg
     3541 gtcgcgtatt agatgttcga ccctatacag gtgattatca tgcacaattt gatgcaagtg
     3601 taattgatga agcgatatca tgttggaaag atgcgccaat agcttatgga ctagatatcg
     3661 gtgttactcg cgatggcaga acacttgttg ttgaagtaaa tgatggttat gcattgggaa
     3721 attatggcct atctccttta aaatcaatca attttcatag agctaggtgg aaagaaatgg
     3781 taaaacccta ttttgaaaaa aatgaaattt ttaaaatcca acaggatgtt attttctaaa
     3841 ataaatatgg tagataacca atataaaaag catcaaaatg aacaaggagg aatctgatgc
     3901 ttggagcaat tattggagat attgtcggtt ctgtttacga atggaacaat atcaaaacaa
     3961 aggactttcc tttatttcgg aaggactgct tttttacaga tgacacggtt atgacctgtg
     4021 ctgttgcaga agcaattatg aatggtggac aaaaagacga tttcattgac gcgatgaaga
     4081 aatatggcag aatgtatccg aatgctgatt acggtgctcg gtttaatgca tggctaaaca
     4141 gcgataaccg tgagccttat aatagctttg ggaatggatc agctatgcgt atttctccat
     4201 gtgcttggat catggactgt ggtttttatg cgaaaactgg tatgtggcca tcatctagag
     4261 gacttacgag tctttctgca gaggtaactc ataatcatcc agaaggtgtc aaaggcgcta
     4321 tggctacagc tgatgctatc tttctatgtc gtttttactt tggtggttat tgtagagagt
     4381 acgagcaatc aattaacgac aatcctacag agtgtaaaag acgaattaag gattatatag
     4441 aaaaagaata cgattacaat ctatctcaaa ctttagatga aatccgtcct aactatcgtt
     4501 ttaacgaaac atgtcaggaa actgtccctc aagccattat cgcctttctt gaaagtagag
     4561 acttcgaaga tgcgataaga aatgccatct cacttggtgg cgacagtgat actctcgcag
     4621 caatcacttg tagcatagca gaggcagctt acggtattcc tgattggatc aaggataagg
     4681 cctattctta cttggatgaa cccttaaagg atgtagttag gcgatgggaa aatagaataa
     4741 aagcgtatta atctccagta cgtcagattt ctcttttctg tattttcatt gtataatagg
     4801 attacattta cttgcatacg acgaaatgta aacatttctg tttaaaatta aggataaaat
     4861 caatgaaaaa agcaatggta attatcaacc ctacttctgg tggcgagaag gctttggatt
     4921 acaaagaaaa gctggagaat aaagcaaaag aatactttga atatgttgaa accaaaatta
     4981 ccgaaaaagc gctggatgca acacattttg ctgaagaagc ttctcgtgag cagtatgatg
     5041 cagtggttgt gtttggtgga gatggaactg tcaatgaagt catttcaggt attgatgaga
     5101 gagactacat tcctaagtta gggattatcc caggcggtac gggtaacctc attacaaaac
     5161 ttttggaaat caatcaagac atcgatggcg caattgacga actggatttt gatttaacca
     5221 ataagattga tatcggtaaa gcaaatgaca actattttgg ttatatcttt agtatcggtt
     5281 ctctgcctga ggcgattcac aatgttgaaa tagaggacaa aacaaaattc ggtattttaa
     5341 cctatgctgt aaataccatg aagtctgtca tgacagatca ggtctttaac attaaggttg
     5401 agacagaaaa tggaaattat gttggtgaag ctagccatgt tttggtcctt ttgacaaatt
     5461 acttcgctga taagaaaatc tttgaagaaa acaaggacga ctatgccaac attttgattc
     5521 tgaaagatgc ctctatattc tccaaattat ccgtcattcc tgatttatta aaaggggatg
     5581 ttgtcgcaaa tgataatatc gagtatatca aagcgcgtaa tattaaaatc tcttcagata
     5641 gtgaattgga gtcagatgtt gacggagata aatcagataa cctacctgta gaaatcaaag
     5701 tcctagctca gcgagtagaa gtattttcaa aaccgaaaga ggattagtat atagagaaag
     5761 ccttttttaa ggctttttgt atactttaaa agatagttcc tttaacaacg gacattcctt
     5821 gcaaatagtt tacaaaaata gtatactgga ttcattgagt ttgaaaacgt ttgcgtaaaa
     5881 tttgaatgaa tactttagga gacaaattga tggaattgag tgctatttac cataggcctg
     5941 agtcggagta tgcctatctt tataaggata agaaactcca tattcgaatt cgaactaaga
     6001 aaggggacat tgaaagcata aacttgcact atggggaccc ttttatcttt atggaggagt
     6061 tttatcagga tacaaaagaa atggtcaaga taacttctgg taccttattt gaccattggc
     6121 aggttgaagt gtcagttgac tttgcacgta tccagtatct ctttgagctc agagatacag
     6181 aaggtcaaaa tattttgtat ggcgataaag ggtgtgtgga aaattctcta gaaaatcttc
     6241 atgcaattgg gaatggattt aagttgcctt atcttcatga gattgatgcc tgcaaggttc
     6301 ctgactgggt ttcaaatacg gtatggtatc agatatttcc tgaaagattt gccaatggca
     6361 atgctctatt aaacccagaa gggactttag actgggattc atctgtcaca cctaagagcg
     6421 atgatttctt tggtggtgat ttacagggga ttattgatca tatggattac ttgcaagact
     6481 tgggtattac tggactatat ctttgtccca tctttgaatc tgcaagcaat cacaagtaca
     6541 atacgacaga ttactttgaa attgaccgtc attttggaga caaggagacc tttcgggaac
     6601 tggtggatca agcgcatcat cgtggcatga aagtcatgct ggatgcggta tttaattata
     6661 ttggttcgca atctcttcaa tgaaaaaatg tcgtcaaaaa tggtgaacag tctgcttata
     6721 aggattggtt ccatattcaa caattcccag tgacaactga aaagctagtt aataagagag
     6781 acttacccta tcatgttttt ggtttcgagg attatatgcc taagctaaat acagccaatc
     6841 cagaggtcaa gaattatctt ttaaaggttg cgacttattg gattgaagag tttaatatcg
     6901 atgcttggcg tttggatgtg gctaatgaga ttgaccatca gttctggaag gattttcgta
     6961 aggcagtttt agctaaaaat cctgatcttt atatcctagg agaagtctgg catacatctc
     7021 agccttggct aaatggagat gagttccatg ccgtcatgaa ttatccttta tctgatagta
     7081 tcaaggacta tttcttacga ggaattaaga agacagacca gttcatcgat gaaatcaatg
     7141 gagagtctat gtattacaag cagcagattt cagaggtcat gtttaatctc ttggattcac
     7201 atgatacaga gcgaatcctg tggacggcca atgaagatgt tcaactggtt aaatcagcct
     7261 tagcctttct ctttttacaa aaaggaacac cgtgcattta ttacggaacc gagctagcct
     7321 tgactggagg accagatcca gattgtcgtc gttgtatgcc ttgggaacgt gtatcaagtg
     7381 acaatgatat gctgaacttt atgaagaggc tgattaagat tcggaaatac gcgtcagtaa
     7441 tcatttcgca tggcaagtat agccttcaag aaatcaaatc tgatctagta gctctggaat
     7501 ggaaatacga aggacggatc ctcaaagcaa tattcaacca atcaacagaa gattatcttt
     7561 tagagaaata agcagtagca ctagcaagca attgccaaga attggagaat cagcttgtca
     7621 tctctccaga tggatttgtg attttctaaa aactagttga tgaagattat ggtacatttc
     7681 atatcttata tagtataata aggctagtta ctaaacttgt aaaggagaac ttaaatgaat
     7741 tgtagaggac atgaaacaag acaaagaatt gttagagatt ttgaagttta gcctaaagca
     7801 catattaagc tgttagcaaa tcaacaaaaa catagtgatg caggagcaac tattgaagat
     7861 gaatattatg tatttatcgc tgagagtaaa attgatggca agaaggaagt tattcagtgt
     7921 tgcatgggtg cggcaaggga ttttttagaa ctaattaatc acaaagggct acctcttttt
     7981 aatccgcttg taggtgattc tcatgtaaat aatagacaag aatatgacaa tacagggagt
     8041 ggaaatttac aacctgaaaa gtggaatgaa actgcaaagc agctttataa tgttataatg
     8101 tggttgatta ttttatggaa tgctaagccg gatacacctt tatttaattt taaagacgaa
     8161 gtaattaagt ataaaacata tgagcctttt gaaagcagta taaaaagagt aaatactact
     8221 ataaagaatg gtagtaaagg gaaaactctg actgagatga ttaatggcta cagagcggat
     8281 aacgatatta gagatgaaat ttgtaacttt aatattctga aaaataaaat tcgtgatatg
     8341 aaagaccaac aaggaaatac aatggaatct tacttttagt tattgttgaa ttttgggtat
     8401 tctataaaat atcctaattg agattt
//
LOCUS       DAVKHZ010000084         8153 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000084 DAVKHZ010000000
VERSION     DAVKHZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.084"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            9..1436
                     /gene="galT"
                     /locus_tag="VWW99_001833"
     CDS             9..1436
                     /gene="galT"
                     /locus_tag="VWW99_001833"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9539631.1"
                     /translation="MYNEIDKIYVKNRVLALIGEEGTDRISDENDLKQIKDYLVEIAL
                     KNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISPQEAVEEFYKLSKDSDY
                     IKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKLVKATNYPKCLLCMENE
                     GYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIFLNSQHVPMAITSKTFE
                     QLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPMEKAKFESEFRFKDFED
                     VNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEVDIIAMTEDVPHHTVTP
                     IARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPPRL
                     KPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVNSVVQHEAGQVFARVLE
                     DAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1449..2429
                     /gene="galE"
                     /locus_tag="VWW99_001834"
     CDS             1449..2429
                     /gene="galE"
                     /locus_tag="VWW99_001834"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9539632.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGLPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
     gene            2639..3298
                     /locus_tag="VWW99_001835"
     CDS             2639..3298
                     /locus_tag="VWW99_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9539633.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3323..4390
                     /locus_tag="VWW99_001836"
     CDS             3323..4390
                     /locus_tag="VWW99_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9539634.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4494..5504
                     /locus_tag="VWW99_001837"
     CDS             4494..5504
                     /locus_tag="VWW99_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539635.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5516..7207
                     /locus_tag="VWW99_001838"
     CDS             5516..7207
                     /locus_tag="VWW99_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9539636.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7210..7920
                     /locus_tag="VWW99_001839"
     CDS             7210..7920
                     /locus_tag="VWW99_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9539637.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2740 a         1142 c         1602 g         2669 t
ORIGIN      
        1 aatacggaat gtataatgaa attgacaaaa tatatgtgaa aaatagagta ctagcgttaa
       61 taggtgagga gggaactgat cgcatttccg atgaaaatga cctgaaacaa ataaaagatt
      121 acttagtgga aatcgcctta aaaaacggta aaattaaaga tttaatcgag gaaaaggaat
      181 gtttaggagc agagttaatg aatttcatag ttcctttacc tagtcgatta aatgatattt
      241 tttggagttc atatgatata tcacctcaag aagcagttga agagttttat aagttaagta
      301 aagatagtga ttatattaaa acttctgcta ttgctaagaa tattgagttt cgagcatcaa
      361 ctaaatacgg agagctagag attacgataa atttgtcaaa acccgaaaaa gatccaaaaa
      421 caattgctgc tgaaaaatta gtaaaagcca ctaattatcc caaatgttta ctatgtatgg
      481 aaaatgaagg atatcagggg cgaataaatt atccagcacg ttcgaatcat agaattattc
      541 gattgaaact tggtgacgaa gtatggggat tccaatattc tccctactta tactttaatg
      601 agcatgcgat atttttaaat agtcaacatg taccaatggc tattacctct aaaacatttg
      661 aacaattatt ggagattgtt gatattttgc caggatattt tgctggttca aattctgatc
      721 ttcctatctc aggaggctcc attcttagtc ataaccacta ccagggaggg aaacatattt
      781 tcccaatgga aaaagcaaaa tttgagagtg aatttcgttt taaagacttt gaggatgtta
      841 atgctggtat tgtaaaatgg ccaatgtcag taattaggtt acaaagtgaa aataaaaatc
      901 gcttgttaga tttagctact aaaattctaa ataagtggag agaatattca gatttagaag
      961 tagacatcat tgctatgact gaagatgtcc cacaccatac ggtgactcct attgctcgta
     1021 aagtggacgg gagatatgaa ttagatattg ttctaagaga taatcatact actgaacagt
     1081 acccagatgg tgttttccat cctcatcaag atgtacaaca tattaaaaag gaaaatatcg
     1141 ggttaatcga agtgatggga cttgcgatat taccacctcg tttgaaacca gagttagaag
     1201 aagttggaaa atatctttta ggagaggata acgcaattgc ggattatcat ttagaatggg
     1261 ccgaccaatt gaaagaaaaa tatcctcgca ttaataaaga agaggttaat agcgtagttc
     1321 aacacgaagc aggacaagtg tttgcgagag ttctggaaga tgcaggagtt tataagaata
     1381 caccgtctgg gcatgaagcg tttatgagat ttgttaagtc ggttggaatt aattaggagg
     1441 aaacaaaaat ggcaatattg gtaacaggcg gagctggtta tattggtagc cataccgtag
     1501 tagaattact aaatttagga aaggaagtca tcattgtcga caacctttcg aactctagca
     1561 tcttggtgct agaccgtatt gaagcaatta caggaatacg tcccgtgttt tacgaattag
     1621 atgtttgtga taaaccagca ttgagaaagg tatttgaaca agaatcgatt gatgctgcaa
     1681 ttcattttgc aggttataaa gctgtcggag aatccgtgca aaagcctgtg atgtactaca
     1741 aaaataatat tatgagtaca ttggcacttg ttgaagtgat gtcagaattt aatgttaaaa
     1801 agattgtctt ttcttcaagt gcgactgtat atggaattaa caatcagtca cctctaattg
     1861 agacgatgca aacaagtgcg acaaatcctt atgggtatac gaaagtgatg cttgagcaaa
     1921 ttttaaaaga tgttcatgtg gcagattcag aatggagtat tgcgttgctt cgttatttca
     1981 atccaattgg tgctcatgag tctggtttga ttggggaaga tccctcagga cttcctaaca
     2041 acttgatgcc ttatattgca caagtagcgg taggtaagct atcagagcta agtgtgttcg
     2101 gaaatgatta tgatacgctt gatggtactg gtgtgcgtga ttatatccat gtagtagatt
     2161 tagcaatagg acatataaaa gctttagaaa aagtatctga aaaaacagat gtttatatct
     2221 ataacctagg ctcgggagaa ggcactagtg tattacaact tgtaaataca tttgaaagtg
     2281 ttaataagat ccctattcct tataaaatag ttccaagacg ttcaggagac gttgcaactt
     2341 gttatgcaaa tgcagacaaa gcatataagg aattaaattg gaggacaaca aaatcgattg
     2401 aagacatgtg tagagataca tggaattagc aatcaaaaaa tcccaatggc tataattgat
     2461 gatactgtct atatgagtgc tgtattttgg aaaaattact taacctttta atgaaatgca
     2521 tatttaatga aagataaatt aaaaaaaatg tttgacagcg cttgacaaag gaaacggttt
     2581 cattgtataa tgtaaacata atgacaccga tgtcaaatat aaaaaagaag gtctttatat
     2641 gaaaaactta acaaaaatta agttcaaaga gaatggggaa tttaatcatt tccctgggaa
     2701 tacagttgta gcaaatcttt atactaaaca agatttgatg gaagttgttg atattattca
     2761 atcacgttat agagaattac catttatcga taagtttact ttaactccaa ggaattctat
     2821 tcacatgact gtaattgaat tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag
     2881 taatcttcct ctagatacac ctttacagga aatacatgat tactttgcta aacaacttga
     2941 aatttttcca ttgttggatg aagaaattca tatgcgtata actgaaatgg gaaaacaaaa
     3001 catactagtt gaacctgcag atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta
     3061 tgtctcagaa aaagcaggtg tttgtttccc taatcatgat agatatcaat tccatatttc
     3121 aattgggtat cttcggattc ctctaaccga agaggaggaa gaagagttta ctaaagtcag
     3181 agcagaatta actgaaattt tattagagaa gattccaact attactgtaa accgtattga
     3241 ctatactgta tttgaagata tgagacaatt tgttccatat cacgaaaaat ttaaataaaa
     3301 taataaaaaa ggagaactta tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac
     3361 gggagttgct cttactacac ttgctgcttg ctctggaggt tcaaattcta cgactgcttc
     3421 ttcatctgaa gaaaaagctg ataaaagtca agaattagtt atctattcga actcagtctc
     3481 aaatggtcgt ggtgattggt taactgctaa agcaaaagaa gctggtttta atataaaaat
     3541 ggttgatatc gctggcgctc aattagcaga ccgtgttatt gctgagaaga ataatgcagt
     3601 tgcagatatg gtatttggaa ttggtgctgt tgactcaaat aaaattagag atcaaaaatt
     3661 actagtacag tacaagccta aatggttaga taaaattgat caatctttat cagataaaga
     3721 taattattat aatcctgtga ttgttcaacc attagtttta attggggcgc ctgatgtaaa
     3781 agaaatgcct aaagattgga ctgaattagg tagtaagtat aaaggtaaat attcaattta
     3841 tggtcttcaa ggtggtacag gacgggcaat tctagcaagt atcttagttc gttaccttga
     3901 tgaaaaagga gatttaggcg tatcagaaaa aggatgggaa gttgcaaaag aatactttgc
     3961 aaatgcctat actcttcaaa aaggtgaaag ctcagttgtt aaagcgttag ataaagaaag
     4021 cccaattcaa tacggtatga tctggggttc tggagcgtta attggacaaa aagaacaaaa
     4081 tgttgaattc aaagttatga ctcctgagat tggtgtacca tttgtaactg aacaaactat
     4141 ggttttaagc actagtaaaa aacaagcgtt agctaaagaa tttattgatt ggtttggtca
     4201 atcagaaatt caagtagaat atagtaaaaa atttggatct attcctgcaa ataaagatgc
     4261 cctaaaagag ttacctgaag atacgaaaaa atttgttgat caagtgaaac cacaaaatat
     4321 tgactgggaa gctgttggaa agcatttgga tgaatgggta gaaaaagctg aattagaata
     4381 cgtaaaataa gaaataagag ttatttttta tgtccaagtc tctctattat ttctaaattt
     4441 aaggaagaaa taaagagagg cttttggttt tagtaataga gggagttagt gagatgatta
     4501 aatttgataa tattcaaatt aaatatggtg attttgttgc aattgataat ctgaatttag
     4561 atatacatga aggggaattc tttacatttc ttgggccttc aggatgtggt aaatcaacta
     4621 ctttgagagc attggtaggt tttttagatc catcatcagg aagtattgaa gttaatggaa
     4681 cagatgtcac tcatttggaa cctgaaaagc gtggaattgg tattgtattt caatcttatg
     4741 cgctatttcc aactatgact gtttttgata atattgcatt tggtttaaaa gttaagaagg
     4801 tagctccaga tgttattaaa gctaaagtat cagcagtggc agcaaaaatt aagatctctg
     4861 atcaacagtt acagcgtaat gtatcagaat tatctggggg tcaacaacaa agggtagcat
     4921 tggctcgtgc tctggttctt gaacctaaaa ttctttgtct agatgaacca ttgtcaaacc
     4981 ttgacgcaaa attacgtgta gatttgagaa aagagttgaa aagacttcaa aaagagttag
     5041 gtattactac tttatatgtt actcatgatc aagaggaagc cttgacttta tctgatagaa
     5101 ttgcagtctt taacaatgga tacatcgaac aggtcggtac accagtagag atttatcata
     5161 attctcaaac tgaatttgta tgtgatttta ttggagatat taatgttttg accgatgaaa
     5221 cagtccacga agtattattg aaaaatacaa gcgttttctt agaggataaa aaaggataca
     5281 ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca agattttatt ctaaaaggga
     5341 caattattga tgttgagttt tctggagtta caattcacta tacaataaaa gtttctgaaa
     5401 gtcagattct taatgtaaca agtattgata gtcaggctgc tattagatct gtcggagaaa
     5461 gtgtggaatt atttatcaca ccatcagacg ttctgcaatt ttaaggaggt gcagtatgcg
     5521 tcataaatta aatttaaaag attggcttat tcgtttaggg ttaatctggt tcttagtaac
     5581 atttattatt tatccaaact ttgatctagt agtgaatgta tttgtaaaag gaggagaatt
     5641 ttcccttgat gctgtacatc gtgttctaaa atctcagagg gcacttcaga gtattatgaa
     5701 cagttttaag ttagcatttt cactcattat tacagttaat gtcgtaggta ttctttgcgt
     5761 tctatttaca gagtactttg atattaaagg tgctaaaatt ttaaaattag gttatatgac
     5821 ctctttaatt tatggaggag tggttttagc gactggatat aaatttgtct atggtccata
     5881 tggattgatt acaaaatttt tacaaaatgt tatcccttct ttagacccta actggtttat
     5941 tgggtatggt gcagtcttat tcattatgac attttcagga actgctaatc atacattgtt
     6001 tttaacaaat acaattcgaa gcgttgacta tcacactatt gaggctgctc gaaatatggg
     6061 agcaaaacca tttactgttt tccgaaaagt agtgttacca accttaattc caactctatt
     6121 tgcacttact attatggttt ttcttagtgg tttatctgca gtagcagcac ccatgattgt
     6181 tggtggtaaa gaatttcaaa ctataaatcc aatgattatt acatttgcag ggatggggaa
     6241 ttctcgtgat ttagctgcct tacttgcaat tattttaggt attgcaacta caattttgct
     6301 tactatcatg aataagatag aaaaaggtgg aaattatatt tctatctcta agactaaagc
     6361 gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc acattgtagc
     6421 atatggattg ttcacagttt tcatgcttcc actaattttt atagtattat actcatttac
     6481 agatccagtt gcaattcaaa caggtaactt aacattatca aactttactt tagaaaatta
     6541 tcgcttattc tttagtaata gtgcggcatt ctctccattc ttggtcagct ttatttattc
     6601 tattattgct gcgacaacag caacaattct cgcagttgta tttgctcgtg ttgtcagaaa
     6661 acataaatct cgttttgatt tcttatttga atatggtgct ctacttcctt ggttactacc
     6721 aagtacactt ttagcagtaa gtttattatt tacttttaat cagccacaat ttcttgtctt
     6781 gaatcagatt ttggtaggta gtttggtaat tctacttatt gcatatatag ttgtaaaaat
     6841 cccattttct tatagaatgg tacgtgctat tttatttagt gttgatgatg agatggaaga
     6901 tgcagcaaga agtatgggtg cttcaccttt ttatactatg atgaaggtta tcattccatt
     6961 tattttaccg gttgttctct ctgttattgc tttaaacttt aactctttat taactgactt
     7021 cgacttatct gtattccttt accatcccct agctcaacca ttaggtatta cgattcgatc
     7081 tgcaggtgat gaaacagcaa catctaatgc acaagctctg gtatttgttt atacaattgt
     7141 tctgatgatc atttctggaa cggtattata cttcacacaa agaccggggc gtaaagtaag
     7201 gaaataatca tgacagccac tagtcttggg ttatcaaata ttgaaatagt tgtcaggatt
     7261 gttttatcag tagtcattgg tagtataatt ggtttagaga gagggagcaa atcccagcct
     7321 gcaggcatcc gaacttatag tattgtttgt ctagctgcat gtttgattat gatgacgaat
     7381 gaatacgtat cttataaatt tgggacagga gatcctacac gattaggagc tcaagttata
     7441 tcaggtgtgg gttttctagg cgctggaacg attcttatta cagataaaaa gaaaattaca
     7501 ggtctgacaa ctgcagcagg catttgggct tcggcaggaa ttggattagc tattggagta
     7561 ggtttttatg agggagctct tttagtagcc atttctgttt ggggtgtgat atccatgttc
     7621 caaccactaa aaaaatatct gcaaaatcgt tctaaaatga ttgaattgta tatagtggtt
     7681 aaatctacag aagcctataa tcgtgtattg gtttattgtg cagaaaatgg tatcagaatg
     7741 accgattcaa gaacagcgtt tggagatgtt aattctgaca gaattgaata ctttgatgtt
     7801 ccggacaaaa aaatagcgtc atttattact ctggaattat caggtagatt tgaacatctt
     7861 cgactgatgg aagaaattgc taatattgta ggtgttattt atgtcgaaga aattagctga
     7921 aatttagtaa acaaagggac agatgttttt gtccttttgt tttttagatg ttttaatcat
     7981 ctaatttttg agtagatttt tgctcagagg tttttatgaa agattataaa atcaattttg
     8041 atttaggtaa aatagaatat tttgataata attgtttgat acaagtatac aaatttattt
     8101 ctttttatga tatttgtgaa atggtgtttg cctttcattt acctcctgat gag
//
LOCUS       DAVKHZ010000085         7789 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000085 DAVKHZ010000000
VERSION     DAVKHZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.085"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..4064
                     /locus_tag="VWW99_001840"
     CDS             <1..4064
                     /locus_tag="VWW99_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9539638.1"
                     /translation="PEKNGTATKPENSGNTTSENGQTEPEPSNGNSTEDVSTESNTSN
                     SNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYKQHISLEQVPSNPN
                     SYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEIESRYKDNFTFYLA
                     KKGTEETTNFTSFSNLVKAINQNLSGTYHLGASLNANEVELSTDDKSYIKGTFTGQLI
                     GEKDGKHYAIYNLKKPLFESLRGATIEKLSLKNVSISGKDDIGSLANEAQNNTKIKQV
                     HVDGVLAGERGIGGLLAKADQSSITESSFKGRIVNTYETTAAYNIGGMVGHLTGDKAL
                     LTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINNVKHFGRVAGVAGN
                     LWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFSSKDNRVYNVTLVK
                     DEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKKDSDFSKVAYYQAK
                     RNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVVMKDNQVITDIVSNKQ
                     TANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQFLYDQTSIIKQVLL
                     DLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLKKLLSADAGLASAN
                     PVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYHLDFFGKGNASPLD
                     TLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKVFLPNTSNNDWFKS
                     ETKAYIVEEKSTIEEVKTKQGLASTKYSIGVYDRITSATWKYRNMVLPLLTLPERSVF
                     VISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDHYDYWYRILDDNAR
                     EKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVGNKVGHNQHGAYAT
                     GDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGPEFFAKGLLQAPDH
                     PDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVHNMFDVVYMLEYLE
                     GNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEEVERLRSFNDLIDN
                     NILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRRIAYELLAAKGFKD
                     GMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQYHTWSEFKKAMYQE
                     RQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKDAEHNYYHWNNYNP
                     DIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            4116..4292
                     /locus_tag="VWW99_001841"
     CDS             4116..4292
                     /locus_tag="VWW99_001841"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9539639.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            4511..5362
                     /gene="ylqF"
                     /locus_tag="VWW99_001842"
     CDS             4511..5362
                     /gene="ylqF"
                     /locus_tag="VWW99_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9539640.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            5349..6128
                     /locus_tag="VWW99_001843"
     CDS             5349..6128
                     /locus_tag="VWW99_001843"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9539641.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILSKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELLKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            6144..7694
                     /locus_tag="VWW99_001844"
     CDS             6144..7694
                     /locus_tag="VWW99_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9539642.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         2570 a         1456 c         1597 g         2166 t
ORIGIN      
        1 aaccagaaaa aaacggaact gcaacaaaac cagaaaattc aggtaataca acatcagaga
       61 atggacaaac agaaccagaa ccatcaaacg gaaattcaac tgaggatgtt tcaaccgaat
      121 caaacacatc caattcaaat ggaaacgaag aaattaaaca agaaaatgaa ctagaccctg
      181 ataaaaaggt agaagaacca gagaaaacac ttgaattaag aaatgtttcc gacctagagt
      241 tatacagttt gtcaaatggt acttataaac aacacatttc gttagagcaa gttccaagca
      301 atccaaatag ctactttgtt aaagtgaaat cttcttcatt caaagatgta tacctaccag
      361 tagcatcaat atcagaggaa agaaaaaatg ataaaatcct ttataaaatc acagcaaaag
      421 tagagaagct tcagcaggag atagaaagca gatataaaga taattttacc ttctatctag
      481 ctaagaaggg aacagaagaa acaacaaact ttacttcctt tagtaatctg gtcaaagcta
      541 taaaccaaaa tctctctgga acctatcatt taggagctag cctgaatgct aacgaagtgg
      601 agcttagtac tgatgataaa tcctatatca agggaacctt tactggtcag ttgattggtg
      661 aaaaagatgg taagcattat gctatttaca acttgaaaaa gcctcttttt gaaagcttga
      721 gaggggccac aatagaaaag ttgagtctga aaaatgtctc tatttcaggg aaagatgata
      781 tcggttcact ggcaaatgaa gctcagaata acacaaaaat taagcaagtt catgtggatg
      841 gtgtacttgc cggtgaacgt ggtatcggtg gtttgttggc taaggcagat caatcaagca
      901 tcacagagag tagtttcaag ggaagaattg tcaataccta tgaaacgact gctgcctaca
      961 atatcggtgg tatggtcggt catttgacag gtgacaaggc tttacttact aagtcaaaag
     1021 cgacagtggc catttcatct aacacaaata cttcagatca gactgtgggt ggacttgcag
     1081 gcctagtaga ccgagatgca cagatccaag atagctatgc tgaaggtgat atcaacaatg
     1141 tcaagcactt tggtagagtc gctggagtgg caggcaattt gtgggatcga acttctggtg
     1201 atgttaggca tgctggaagt ttgaccaatg ttctcagcga tgttaatgta accaacggaa
     1261 atgccatcac tggttaccac tataacgaaa tgaaggtaaa ggacacattc agcagcaagg
     1321 acaacagagt ctacaatgtc accttggtca aggatgaggt cgtcagcaag gaatcctttg
     1381 aagaaagagg aacaatgcta gatgcttctc aaattgcaag caaaaaagca gaaatcaatc
     1441 ctctcattct accaacagtg gagccacttt caacaagtgg caaaaaagac agtgattttt
     1501 ctaaggtggc ctattatcaa gctaagcgca acttgactta taaaaacatt gaaaaattgc
     1561 tacctttcta caacaaggca accatcgtca aatacggaaa tctggtcaat gagaacagtc
     1621 ttttatatca aaaagaactc ttgtcagcag tcgtgatgaa ggacaaccaa gtcatcacag
     1681 acattgtttc taacaaacag actgcaaaca aactcttgct tcactacaag gatgatttat
     1741 ctgagaagct ggatctcaaa taccagaatg atttcgccaa attagcagaa tatagtctgg
     1801 gcaatactgg acttctctat acgccaaacc aattcctgta tgaccaaacc tctatcatca
     1861 agcaagtctt acttgactta caaaaggttg actatcattc agaagccatc agaaagacgc
     1921 tgggtatttc tccaaacgtc aagcaaactg agctctatct agaagaccag ttcgccaaaa
     1981 caaaacaaca actggaagac agtttgaaaa aactcttgtc agcggatgct ggactggcta
     2041 gtgctaaccc agtcactgaa ggttatcttg tagataaaat caaacgcaac aaggaagcct
     2101 tgctacttgg cttgacctat ctggaacggt ggtataactt tagctatggt caggtgaatg
     2161 tcaaagacct agttctgtac catttggact tctttggtaa ggggaatgct tcaccattag
     2221 atactctgat tgagttgggt aaatctggct ttaacaatct tctagctaag aataatgtcg
     2281 atacttatgg tatcagtctt gccagtcaac atggaacgac agatttgttt agcacgctgg
     2341 aacattaccg aaaagtcttt ttaccaaata caagcaataa tgactggttt aaatcagaga
     2401 ctaaggctta cattgtcgaa gaaaaatcca ctatcgaaga ggtgaaaacg aagcaagggt
     2461 tagctagcac caagtattct atcggtgttt atgatcgtat cacgagtgcc acatggaaat
     2521 accgcaatat ggtcttgcct ctcctgacct tgccagagag atccgtattt gtcatctcga
     2581 ccatgtctag tctaggattt ggagcttatg atcgctaccg cagtagtgac cataaagcgg
     2641 gcaaggctct caatgatttt gttgaagaaa atgcgcgtga aacagccaaa cgtcagcgag
     2701 atcactacga ttattggtat cgtattttag acgacaatgc acgtgaaaaa ctttatagaa
     2761 atattttgct ttacgatgct tataaatttg gcgatgataa taccgtaggg aaagctacag
     2821 aagtggcaga ttttgataat ccaaatcctg caatgcaaca tttctttgga cctgttggaa
     2881 ataaagttgg gcataatcaa cacggtgctt atgctacagg tgatgcagtt tattatatgg
     2941 gttatcgaat gttggataag gatggagcta ttacttatac gcatgagatg acacatgact
     3001 cagatcagga catttatctt ggaggatatg gtcgaagaag tggcttggga ccagagttct
     3061 ttgctaaagg attattacaa gcaccagacc atccagatga tgcgaccatt accatcaact
     3121 ccatcttgaa acattcaaaa tctgatagta cagaaagtcg acgattacaa gtacttgatc
     3181 caactacaag atttaataat gcagatgatt tgaagcaata tgtccacaac atgtttgacg
     3241 ttgtttatat gttggaatat ctcgaaggaa attcaattct taaattggat acgaatcaaa
     3301 aacaacaact tcttagaaaa gttacaaatg agtaccatcc tgatcctgat ggaaataagg
     3361 tctatgcaac aaatgttgtc agaaatctaa cagtagaaga agttgaaaga ctacgttcat
     3421 tcaatgattt gattgataat aatattcttt cgtctaggga atatgcctca ggtaaatacg
     3481 aaagaaatgg ctacttcact attaagttat ttgcaccgat ttatgctgca ttaagtaatg
     3541 atataggaac accaggtgac ctgatgggac gtcgtatagc ctatgaacta ctagctgcta
     3601 aaggctttaa agatggtatg gtaccatata tctcaaacca atacgaagaa gaagccaaac
     3661 aaaagggcaa gacaatcaat ctctacggta aaacaagagg tttggttaca gatgacttgg
     3721 ttttggaaaa ggtatttaat aaccaatatc atacttggag tgagtttaag aaagctatgt
     3781 atcaagaacg acaagatcag tttgatagat tgaacaaagt tacttttaat gatacaacac
     3841 agccttggca aacatttgcc aagaaaacta caagcagtgt agatgaatta cagaaattaa
     3901 tggacgttgc tgttcgtaag gatgcagaac acaattacta ccattggaat aactacaatc
     3961 cagacataga tagtgaagtc cacaagctca agagagcaat ctttaaagcc tatcttgacc
     4021 aaacaaatga ttttagaagt tcaatttttg agaataaaaa atagtgtcta ctattaggaa
     4081 ataaagttta aaaaggtgat gaagaacaaa ccaagattca agcaggaatt cctactgata
     4141 atgaagtaag ttatgatctt atttatcagc aggaaactct tcctgcaaca ggttcatcaa
     4201 cttctgagct tacagcttta ggcctattag ctgttggtag tttagttctt ttggttcata
     4261 atatgacggg aacagttttt tgctccctct gaaaagtcat catttgatgg cttttttcta
     4321 tatagggtaa aagatagggt aaaaggctat catcggacaa aataaagaag gcatgatata
     4381 atataaagta gatttctatg tcataaaaca agaactgttt ggacatcatt catttgaaaa
     4441 ctctctatgt tcaaacaata gtaaaataaa ataggggatc taaatccttg ctatgaaagg
     4501 aaaaaactca atggctacta ttcaatggtt tcctggtcac atgtctaaag ctcgtcgaca
     4561 ggtgcaggag aatttaaaat ttgttgattt tgtgacgatt ttagtagatg cacgcttgcc
     4621 tctatctagt caaaatccta tgttgaccaa gattgttggt gataaaccaa aactcttgat
     4681 tttaaacaag gccgacttgg ctgatccagc aatgaccaag gaatggcgtc agtattttga
     4741 atcacaagga atccagacgc tagctatcaa ctccaaagag caagtgactg taaaagttgt
     4801 aacagatgcg gccaagaagc tcatggctga taagattgct cgccagaaag aacgtgggat
     4861 tcagattgaa accttgcgta ctatgattat cgggattcca aacgctggta aatcaactct
     4921 gatgaaccgt ttggctggta aaaagattgc tgttgttgga aacaagccag gggtcacaaa
     4981 aggtcaacaa tggcttaaaa ccaataaaga cctggaaatc ttggatacac cggggattct
     5041 ctggcctaag tttgaggatg aaactgttgc acttaagttg gcattgactg gagctatcaa
     5101 agaccagttg cttcctatgg atgaggttac catttttggt atcaattatt tcaaagaaca
     5161 ttatccagaa aagctggctg aacgcttcaa acaaatgaaa attgaagaag aagcgcctgt
     5221 gattattatg gatatgaccc gcgccctcgg tttccgtgat gactatgacc gtttttacag
     5281 tctcttcgtg aaggaagtcc gtgatggcaa actcggtaac tataccttag atacattgga
     5341 agacctcgat ggcaacgatt aaagaaatca aagaattcct tgtgacagtc aaggagttag
     5401 aaagccctat ttttttagag cttgaaaagg ataatcgctc aggagttcaa aaggaaatca
     5461 gcaagcgtaa aagagccatt caagctgaat tagatgaaaa tttgcgcttg gaatccatgc
     5521 tttcttatga aaaagaactt tataagcaag gattgacctt aattgcaggt attgatgagg
     5581 ttggtcgtgg tcctcttgct ggtcctgtag tcgctgcggc cgttatttta tctaaaaatt
     5641 gtaagattaa aggtctcaac gacagcaaga aaattcctaa aaagaaacat ctggagattt
     5701 tccaagccgt tcaagaccaa gccttgtcga ttggaattgg tatcatagat aatcaggtca
     5761 tcgaccaagt caacatctat gaagcaacca aactagccat gcaagaagca atctcccagc
     5821 tcagccctca accagagcac cttttgattg atgccatgaa actggacttg cccatttcac
     5881 aaacctccat tatcaaagga gatgccaact ccctctctat cgcagcagca tctatagtag
     5941 ccaaggtaac acgtgatgaa ttgctgaaag aatacgatca gcagttccct ggctatgatt
     6001 tcgctactaa tgcaggatat ggcacagcta aacatctgga aggcctcaca aaactaggag
     6061 ttaccccaat tcaccgaacc agctttgaac ccgttaaatc actggtttta ggtaaaaaag
     6121 aaagttaatt gaaaggaaat aacatggagg aacagtcgga aatagtccgt tctaagaaag
     6181 aattcgcctt tgcatccagc actatactat cccaagttgg tcgaggaatc attgtcggcc
     6241 tcatcgttgg aattatcgtc ggatcctttc gtttcttaat tgaaaagggc ttccacctga
     6301 tacaaggagt ttatcaagat caagggtact tagtgcgcaa tctttttgta ctggttttgt
     6361 tttatatact catctgttgg ctcagtgcca aactaacacg gtcagaaaaa gatattaaag
     6421 gctcaggaat tcctcaagtc gaagccgaac tgaaaggcct catgtccctc aactggtggg
     6481 gcattctttg gaaaaaatat gtgctaggta ttcttgctat tgccagtgga ctcatgctgg
     6541 gtcgagaggg acccagcatt caacttggag cagttggtgg taaaggaatt gccaagtggc
     6601 tcaaatccag tccagtagag gaacgttcct tgattgccag tggagctgca gcaggtttag
     6661 ccgcagcctt taatgctcct attgcagcac ttctctttgt tgtagaagaa gtctatcacc
     6721 atttttcgcg ctttttctgg gtctcaactc tagcagccag catcgtagca aactttgtgt
     6781 ctctactcat gttcggtttg acaccagtat tggatatgcc agataacatt cctcccatga
     6841 ccctagatca gtattggata tatctcgtca tgggaatttt ccttggattt tcaggttttc
     6901 tctatgagaa agctgtatta aacgttggaa gagtttatga cttgattggt caaaaaatcc
     6961 atttggatag ggcttattat cccatcttgg cttttatcct tatcatacca gtcggaatct
     7021 tcttacctca aatcattggt ggcggaaatc agcttgtcct ttctttaact gaacaaaatt
     7081 ttagtttcca agttttatta gcttactttt taatccgctt tatttggagt atgattagct
     7141 atggaagtgg actgccagga ggaattttcc tccccatttt agctcttggt tctttgcttg
     7201 gtgccttagt tggtgttatc tgtgtcaatc ttggacttgt cagtcaagag caattcccta
     7261 tatttgtcat tctaggaatg agtggctatt ttggagccat atcaaaagct cccttaaccg
     7321 ctatgatcct cgtaactgag atggtaggag atattcgcaa ccttatgcca cttggtcttg
     7381 tcactcttgt ttcttatatt atcatggatt tgctcaaagg tacgccagtc tatgaagcca
     7441 tgctggaaaa aatgcttcca gaagaagtat ctagcgaagg agaagttaca cttatcgaaa
     7501 taccagtttc tgataaaatt gctgggaaac aagttcatga actcaactta ccacacaacg
     7561 tcctcatcac aactcaagtc cataatggca agagccaaac agttaacggc tcaaccagaa
     7621 tgtatctggg tgatatgatt cacctggtta ttccaaaaag tgaaattgga aaagtcaaag
     7681 atttgttgtt gtagtatgag tatttacata atttatgtta tgtaaatgat cagtttgatt
     7741 tatttagaaa accgattctc aggaatgaga tcggttattt tttactgat
//
LOCUS       DAVKHZ010000086         7357 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000086 DAVKHZ010000000
VERSION     DAVKHZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.086"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(201..1592)
                     /gene="argH"
                     /locus_tag="VWW99_001845"
     CDS             complement(201..1592)
                     /gene="argH"
                     /locus_tag="VWW99_001845"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9539643.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDVIGSLAH
                     VQMLGQTGILSLEESEKIQVGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGYVLDKLAHLKGVLLDLAENHVATIMPGYTHL
                     QHAQPISFAYHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKQPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TIGSSIMPQKKNPDMAELIRGKTGRVYGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(1621..2817)
                     /locus_tag="VWW99_001846"
     CDS             complement(1621..2817)
                     /locus_tag="VWW99_001846"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9539644.1"
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDF
                     IHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVE
                     IAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
                     GVPIPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEI
                     EFSEGVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAV
                     TLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQALIAYIKETQKVVN
                     GTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSE
                     VQKSAK"
     gene            complement(2834..3640)
                     /locus_tag="VWW99_001847"
     CDS             complement(2834..3640)
                     /locus_tag="VWW99_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9539645.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(3847..4488)
                     /locus_tag="VWW99_001848"
     CDS             complement(3847..4488)
                     /locus_tag="VWW99_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539646.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(4485..6593)
                     /locus_tag="VWW99_001849"
     CDS             complement(4485..6593)
                     /locus_tag="VWW99_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9539647.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHALLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6645..6932)
                     /locus_tag="VWW99_001850"
     CDS             complement(6645..6932)
                     /locus_tag="VWW99_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9539648.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
BASE COUNT         2225 a         1675 c         1213 g         2244 t
ORIGIN      
        1 ctgccaagcc acatagtgtg tttactacta tgtgtccatg tcaccatgaa tcgccgaacg
       61 atttaacgag aaagtatgac ttttacgttt atcccaactc aattatgaca tttttttcaa
      121 aagtcaatat atctcacttt ttcaacgaca agaaagaggc tgccaatcga ccaacctctt
      181 attctgaacc catcactcca tcacttttta gcttcattcg ctttcttagc gactgcaatc
      241 tggtattcga cttggtcaaa ccctgtgcct cccaaggaat ttcgtctttg aacagcagtt
      301 ttaggttgca agtagacata gatatcctcg gtaatgagag aatgataggt ttgtaattcc
      361 tccaaagtcc aatcttgaag atttttagca gacttgatag agtctagcac taatcttccc
      421 acaacctcat gagcttctct aaatggcaag cctttccctg ccaaataatc tgccaactca
      481 gttgcatttg aaaagtcctt ctctgttgat tcttgcattt tttccttgtt tacctgcaaa
      541 ctagataaca tacctgccaa cacatcgagg gaatttaaaa tcgtttcgac cgtatcaaac
      601 attccttcct tatcttcttg caaatcctta ttataagcta aaggcaagga cttcatgact
      661 gtcaacagtc caaacaaatg cccataaact ctgccagtct ttcctcgaat caactcagcc
      721 atatctggat ttttcttttg gggcataata gaggaaccta ttgtaaacgt atctgacaag
      781 gtaatgaatt ggtactcaaa actacaccaa ttaatcattt cttcgcaaaa acgactcata
      841 tgcatcatga gtatgctggc atttgataga aattctaaga taaaatcgcg gtcactaaca
      901 gcatccaaag aatttgtata aggttgctta aactccagca aatcgctaga caattggcga
      961 tcaatgggga aagtcgttcc tgccaaagct gccgcaccta aagggcatag gtctgtatgc
     1021 ttctggttaa attcaaaacg ttcactgtct ctttgaaaca tattgtagta agccatcagg
     1081 tggtaggcaa aactaatagg ctgggcatgt tgcagatggg tatagcccgg catgatcgtc
     1141 gccacatgat tttccgccaa gtctaataaa acacccttaa gatgagccag tttatctagg
     1201 acatagccta gttgttcctt gagatacaag tgcatatctg tcgcaacttg gtcatttcga
     1261 gaacgagccg tatgtaactt ccctgcaagg ggaccaattt tttctgtcag caacacttcc
     1321 atattcatat gaatatcttc gtttgcgata tcaaagtcaa gttgacctgc ctctagctct
     1381 tctaaaagct ctttcaagcc tacttgaatc ttttctgact cctccaaact caaaatgccc
     1441 gtctgaccca acatctgaac gtgggctaaa gaaccaatca catcaaattt agctaatttc
     1501 tggtcaaaag atatactcgc accaaagcgc tctacccagt cttcgacagt accttcaaag
     1561 cgaccacccc ataattttgt attcttagcc atatcacatc cacctctcta tccaatcgtc
     1621 ctatttggcg cttttttgaa cctcagaatg aaccttggtt ggaagtcccc ataacttaat
     1681 aaagccaaca gccgcatctt ggtcaaaggt atctgcacta gtatacgtcg ccaaattttc
     1741 atcgtaaaga gaacttggtg atttccgagc cacgacctga gcgctcccct tatacagttt
     1801 gacttttgca gtcccattga caactttttg agtctcttta atataagcaa tcaaggcttg
     1861 tgtagccgaa ctaaaccaca aagcattata aatgagattt gataattcat tttctatgat
     1921 tggtttaaaa tgagccactt ctctcacaag cgtcaagtcc tcaatttcct tatgagctgt
     1981 cagcagggtc actgcacctg gacactcata gatttctctt gatttaatcc caaccaagcg
     2041 attttccacg tggtcaatcc gtccaacccc atgttttcca gctatttcat tgagtttttg
     2101 aatcaaatct gccaatttta acacttctcc attgagggaa actggaacac cttcactaaa
     2161 ctcaatctca atgtattctg gcatatctgg agcttgctct ggagaagttg tgatgccaaa
     2221 ggcttcttct ggagcctgat tccatggatt ttccaaaata ccacactcat tggcacgtcc
     2281 ccaaagattt tggtcgacag agtagggatt gtcaaggtca gcaggaatag gcaccccatt
     2341 ttccttggca taataaattt cttcctcccg agaccatttc cattcacgaa ctggagcaat
     2401 cacttttaaa ttgagatcca aggctgcaat cgatacttca aaacgtactt ggtcattccc
     2461 cttaccggta caaccatgag caattgtagt cgctcctatc tgatgcgcta tttcaaccaa
     2521 ttttttagaa atcagagggc ggctcaaggc agataccaag ggatactttt gttcataata
     2581 ggcatgtgac tgatgagcca ctagcacata atctgtagca aattcgtcct taacatcaat
     2641 gacataagat tcgactgccc caaccttaag agccttatca tggatgaaat ctaagtcttt
     2701 cccttcaccc acatccatac aaactgcaac aacatcatag tctttcttca gccaggtaat
     2761 agcaactgat gtgtctaaac cgccagaata ggccaaaatt actttttctt tactcatgtt
     2821 cttttccttc tttctatttt tcaatggacg ctttaaaggc atcctcaatg agtttgtcta
     2881 attccccaga ctccttcaac ttttgaatgg ttttatcgac tgcctctttc aattccttgc
     2941 tatctttttt catggctacc gcataggaat catcttgctc tttttcaaaa ttgaggtctg
     3001 cgattgctaa atcaggatta ttttccacaa atcccttggc aacgggttct tcaaaaataa
     3061 cggcatccac ttgccctgat tttaaatctg tgattaaatt cccattttta ggcagagata
     3121 cgagggaaga attttgtagc aaatctttcg ccatcgtctc ttgaatcgaa cctttctgcg
     3181 ctccgacctt tttctgcgcc aaatcgttga cagactgata agtggctaaa tcagattttt
     3241 tgacaatgag tttatttttt gcagtatagt agggagttga aaaatcaaac accttgctcc
     3301 gttcatctgt cttagaaaca cctgatatgg caaggtcagc ttttcctgat tgaacgctag
     3361 ccagtacatt gtcaaaactc ataggagata gttccaattc gacacctagt tctgttgcga
     3421 tagccttggc taattcgata tctgaaccca caatctgatt tttcccatca accacttttt
     3481 ggtattcaaa tggagcaaaa tctggattga gggctacaac caattttcct ttggacttga
     3541 tggcttcaat tccagccgat tgattgttgc tacaagcaaa tagtaggggg agcatcacta
     3601 aaccaaacat cgtcatcaac accttcttca tcttattcat aacaaagtct cctttatgtt
     3661 tattcttttt ggttgtataa ataatactcc tatactcatc ctttgtcaag cctaaattga
     3721 aaaaattttc aatttgaaac aaaaaaaacc tagaaatact acataaaatg tcattcctag
     3781 gcgtatttat gctatttttc tctggaaaac atgagtattc agccagtcaa aaggagctga
     3841 atgaactcat tttccctcgc ccaattcaat gacacgatga cattgttggg ctacataagc
     3901 atcgtgggtc acgataatga ctgttttccc ttctcgattc atctctaaga gaaacttcaa
     3961 gaccaaatct ctattttcag gatccagcga acctgtaggt tcatcggcta aaatcagctg
     4021 gctgggtttt aagatggctc tagcaactgc aattcgttgt tgttcgcccc cagacaactc
     4081 ggagaccctt tgatgcaaag tagctgacaa acctactctc tctaaaatct cttccacctt
     4141 tttgagcttg tctttcttag gcaatttcac atatttcagc gccagcatga gattgtactc
     4201 gaccgtttca tcatcaatca gggcaaaatt ttgaaacaga taagagatat gttcacggat
     4261 tattgtttgc gacttagcag aattaaccgc tagatttgtc tgaccaaaaa tctcataccg
     4321 tccgctataa tcaccatcta tcaaacccaa taaatttaac aaggtcgact tcccactacc
     4381 actcttacca acaatagcta ccaaatcccc ctgatcaatc ctgagagata agttatccaa
     4441 aatcactttt cccccaatgg ttttggtaat atttttcaac tcaatcataa gatgccccct
     4501 ttcaataact ctactagact tcttttctcc atcctagaag ctaagcctag cacaaatagt
     4561 atatccagac atgtaaaacc tgcaaacagc aggagtggca agagcgcatg ggcaaagaaa
     4621 atcaagacta gaagagggaa actatagccc agcaagagca gaacgaggag aggacggtag
     4681 cgatcgacca gtttccaccc cataaacttc ttggtaatga tatccctgcg cttcaataag
     4741 aaagttgtta ctagtaagaa gtaggaaatc atcatgctaa ggagaccaaa caaagcaaag
     4801 agtaggttaa aattccgaac agcatctcga taagaatcca ctttctcttg ttgaatggct
     4861 tgaatagatg aaaattttaa ataatttcca tctgacaatt tctcaactaa ctctgtaatc
     4921 tctttttgat gttgaaccgt attttcaatt ttaatcggat tatttaagcc agttgttgac
     4981 agggaggctt tctcatccca catcatatca gaatcattga ccaagctaat aattggattg
     5041 gagagatttt cctttcgctt atcactatat gggaaaaatg accaatctcc ttcataatag
     5101 gcaatctcga catccatctc ctctatcgtt cgtttttgct gctcttcata cttcatcgaa
     5161 tgaaaggcaa ttaacttccc caagagctga tttttatctt cttcaccttt cgtacttgct
     5221 ggcatcaaaa taactttttt aataccggta tctggtagct tgaatccctt gctctttaga
     5281 aaattgcgat tggcatagta aacatccacc gtatctgtta actgatattg ctgaatctgt
     5341 tctgattgga caaaattttt tacaggaaga ctgctactct gcacatagcc cgcctgcgtt
     5401 ttttctacca aatcctgata aaatcgatag aaataatctg tagatttccc tgaccctgct
     5461 agctcttctt gccacagatt atcattgagt ttgaaggttt ctaaggtcag gtaattacct
     5521 tgacttaccc actgttgctg ataagcaagt tctttgtttt cttgttctaa acttctgccc
     5581 accccaatca gtaaggccgt cagtaaaata gttgtcccta ttttcatcac ataattgaag
     5641 ataagaccaa atttgaaaga tgaaaaacct ttcagcagag agctgattgt cattttttgg
     5701 attaagaggt aagtcaacca actgataaag agataaagct gcaacagcaa aaaatgagac
     5761 aaccacagca taggaaacaa atcttttggc ttataatcaa gcaagaaaaa cacgcctaga
     5821 ttgatcacaa gagccccacc taggaggagg taaaggttgc cttttacaac atcagctaaa
     5881 acagccctat cttgaaaacc aagtaatttt tgtaccccaa ctcttttcat ctccatcatc
     5941 ggttgataca ctgtcactaa cacaagaagc aaaatagcca agacaaaaac aatggcagat
     6001 aaaagcaaat ctcgatttat gacttccact gcacttttgt aggtcggctc tagcaaggta
     6061 gcctggtcta tcttgaaaaa atcgctccat ttctgtacaa tcctatcctt gtccatctct
     6121 tgtgtagaag ttatcgtata gcgaccattt aaactacgag atgtatcctt gatataggtt
     6181 tgaaaagtca taagctgaat aggtttggct tttagaaagg tcggaatcgt accaagttta
     6241 ttggaaattt ctttattact atagactcct tcaccatctg tggtaaaatc aagagaagaa
     6301 atcccaaact cttggtaggg gaaggtatct ttatcaaaaa caccagactt gaccacctca
     6361 tcaccactgt ctgttttgat gatggagact ttatactcct ttgatacatc ctcaaaaaat
     6421 cgaagaacag acgctgcagg ttcgttaata tctttcaaat acaaatccaa agaatctaca
     6481 gtctttccca tttcttgaat ccgaaccact tctcgtgtat agtttacatt aaaaacaaaa
     6541 aaagtggtac acagaagcag gagtaacata cagaaattac tgattttttt cataaaagtg
     6601 cctccactac agttttccct ctccaaatag gagagggaag aattttactc aaatccatag
     6661 aaatattcca ttcctgttgg cggaatcttg gtgagggaag ctctagccca tgcctcaggt
     6721 tttgcataat ccttagaggt tctaccccca tgtctaacag ttgctgtatg atatcgagta
     6781 gaatgtaagt aatcagaata tccaaaagtt cctgtccaac ctactccata gttccattga
     6841 ccaccctcaa cccatactgc taaagcattt gtagtagcaa gtataccaca taccattaaa
     6901 gatgcaacac caattttaac taattttctc atgatttatt tcttctatta tcgagagctg
     6961 ttccgtaaaa acagctatag taatttatca acaagtatcg tagtccattg gactcacctt
     7021 ccttctatct catttcattt tttaacaacg aacttactct taaccgggag aatggtgcgc
     7081 ttctgtatca ggtccttgaa caaccagagt agtattaagc acactctaac tattactcaa
     7141 gaatcagtcc aataaacaat atggatagcg ctttcaaatt tttaaactcc tatcccgtcc
     7201 ttttatctat ataataagtg aaaatataat aactgtcaag taatcgaagt gaattttata
     7261 aaaaaaaatt acaagccaaa tttgcaaagt ttacaataag ccgctagggc aatcgtctat
     7321 cagaatgtcc gtttatttgt caataatccg agaaaat
//
LOCUS       DAVKHZ010000087         7195 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000087 DAVKHZ010000000
VERSION     DAVKHZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.087"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            86..646
                     /locus_tag="VWW99_001851"
     CDS             86..646
                     /locus_tag="VWW99_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9539649.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            703..1611
                     /locus_tag="VWW99_001852"
     CDS             703..1611
                     /locus_tag="VWW99_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9539650.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            1563..2153
                     /locus_tag="VWW99_001853"
     CDS             1563..2153
                     /locus_tag="VWW99_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9539651.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            2156..3262
                     /gene="yqeH"
                     /locus_tag="VWW99_001854"
     CDS             2156..3262
                     /gene="yqeH"
                     /locus_tag="VWW99_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9539652.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTDDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAREEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            3477..3788
                     /gene="yhbY"
                     /locus_tag="VWW99_001855"
     CDS             3477..3788
                     /gene="yhbY"
                     /locus_tag="VWW99_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9539653.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            3840..4469
                     /locus_tag="VWW99_001856"
     CDS             3840..4469
                     /locus_tag="VWW99_001856"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9539654.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            4469..5062
                     /gene="yqeK"
                     /locus_tag="VWW99_001857"
     CDS             4469..5062
                     /gene="yqeK"
                     /locus_tag="VWW99_001857"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9539655.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            5063..5563
                     /locus_tag="VWW99_001858"
     CDS             5063..5563
                     /locus_tag="VWW99_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9539656.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            5576..5929
                     /gene="rsfS"
                     /locus_tag="VWW99_001859"
     CDS             5576..5929
                     /gene="rsfS"
                     /locus_tag="VWW99_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9539657.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            6249..6989
                     /locus_tag="VWW99_001860"
     CDS             6249..6989
                     /locus_tag="VWW99_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9539658.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNVLNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFSMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            7030..>7195
                     /locus_tag="VWW99_001861"
     CDS             7030..>7195
                     /locus_tag="VWW99_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539659.1"
                     /translation="MNIPIPFGDILEKEVITNIPKIYQKLKQIYNDLQFKTEEELGVV
                     YQEYLQFTYKK"
BASE COUNT         2168 a         1397 c         1624 g         2006 t
ORIGIN      
        1 atcaagaatt ttctgtctat tttttgaata tttatggaga atgagactga tgaaaatatg
       61 gtataatgaa ataaaggagt tttatatgca aaaatttatt caggcttata ttgaaaagct
      121 agatgtgaca accattatcg agaatattct aaccaaggtc atttctcttt tactgctttt
      181 gattgtattt tatattgcta aaaaaatgct tcataccatg gtgcagagaa ttgtcaaacc
      241 ttctctaaaa atgtctcgtc atgatgtcgg acgccaaaaa accatctcac gtttactaga
      301 aaatgtgttt aattatacgc tatatttctt tttactctac tgcattttgt cgattttagg
      361 tttgccagtt tctagtttgc tggctggagc tggtattgct ggggtagcga ttggtatggg
      421 agcccaaggc tttctatctg atgtcatcaa tggctttttc atcctctttg aacgtcaact
      481 ggatgtggga gatgaggtcg ttctgacaaa tggaccgatt actgtatcgg gtaaggttgt
      541 cagtgtggga attcgtacga cacagcttcg tagcgaggag caagcccttc actttgtccc
      601 taaccgaaat atcacagttg ttagcaattt ctcacgcaca gactagacct gttattttaa
      661 gtaatttgtg gtacaataga gggagtttaa taaggagaaa agatggtttt agaaaagcag
      721 ttgggcaatg gttgtacctg gatagaccta gacctaggaa agttgaataa actagaagac
      781 ctttctgaaa tttacggttt ggacaaggaa accattgaat acgcactgga tagaaacgag
      841 cgcgcccaca tggactacca ccgtgaaagt gggacggtta cctttatcta taatgtccta
      901 gacgtaaaaa aggacaaggc ctactatgag acttttccca tgacctttat cgtcgagcat
      961 cgtcgcctga ttaccattag taataccaag aacgcctatg tcattgaaca gatgactcgt
     1021 tatctggaga accatgacac gctttcgatt tataagtttc tctttgccag tctggaaatc
     1081 atcagcaatg cctactatcc tgtcattgag cagatggaca agagtaggga tgaggtcaat
     1141 gacctcttgc gccagcgaac taccaagaaa aacctctttg ccctgtctga tttggagact
     1201 ggtatggttt atctgacggc agctgccaaa caaaatcgga ttttgttaga gcatattcaa
     1261 ggtcatgcct tgtatcgtag ttttgatgag attgagagag aacagtttga tgatgccatg
     1321 attgaggctc atcagctggt atccatgaca gacctaatct ctcagatttt acagcagctt
     1381 tcagcctctt acaacaatat tctaaacaat aatctgaatg acaatttgac aaccttgact
     1441 atcatttcag tcttgctagc tgttttggca gtcgtgacag gctttttcgg aatgaatgtt
     1501 cccttacctt taacagatga gccccatgct tggctctata tcagtttggc tagtgcaggt
     1561 ttgtggattg ttttatcctt gttactaagg aaaattgcga aaaaaagtta agaaaaggag
     1621 ccagaatggc gattgaaaat tatataccag attttgccgt ggaagcagtc tatgatctga
     1681 cagtcccaag cctgcaggcg cagggaatca aggctgtttt ggtcgatttg gataataccc
     1741 tcattgcttg gaacaaccct gatggaacgc cagagatgaa gcaatggcta catgaccttc
     1801 gggacgcggg tattggcatt atcgtagtgt caaataacac caaaaaacgc gttcaacgag
     1861 cagttgagaa atttgggatt gattacgttt actgggcctt gaagcccttc acatttggta
     1921 ttgaccgtgc tatgaaggaa ttccactatg acaaaaagga agtggtcatg gttggcgacc
     1981 agctcatgac agatatacga gcagcccacc gtgcagggat tcggtcaatt ttagtcaaac
     2041 ccttggtcca acatgactca atcaaaacgc agattaaccg aactcgtgag cgtcgtgtta
     2101 tgagaaaaat cactgaaaag tacggaccga ttacatataa aaaaggaatt taactatgga
     2161 agaaattctc tgtattggtt gtggagcaac cattcagacg acagataagg ctggtcttgg
     2221 ttttaccccc cagtcggcac ttgaaaaagg tttggagact ggcgaagtct attgccaacg
     2281 ctgtttccgt ctccgccact acaatgaaat cacagatgtc cagttgacgg acgatgattt
     2341 cctcaagctc ttgcacgagg tgggagacag tgatgcctta gtggtcaatg tcattgatat
     2401 ctttgatttt aatggatctg tcatcccagg tttaccacgt ttcgtctcgg gcaatgatgt
     2461 cctcttggta ggaaataaaa aagatatcct tcctaagtca gttaagtctg gtaagattag
     2521 ccagtggctc atgaaacgtg cccgtgaaga aggtcttcgt ccagtcgatg tggtcctaac
     2581 ttcagcacaa aataaacatg ccattaagga agtcattgac aagattgaac actaccgtaa
     2641 gggccgcgat gtctatgtgg tcggtgtgac caacgttgga aaatcaactc taatcaatgc
     2701 tattatccaa gaaatcacgg gtgatcagaa tgtcatcact acttcacgct tcccagggac
     2761 aaccttggac aaaatagaga ttccgcttga cgacggatct tatatttacg atacgccggg
     2821 aattatccac cgccaccaga tggctcacta cttgacggcc aaaaacctca agtatgtcag
     2881 tcctaaaaag gaaatcaagc ctaagaccta tcagcttaat cctgagcaaa ccctattttt
     2941 aggtggtttg ggacgctttg actttatagc aggagaaaag caaggattta ctgctttctt
     3001 tgataatgaa ctcaaactcc atcgtagcaa gcttgaagga gctagtgctt tctacgataa
     3061 gcacctggga actcttctga caccaccaaa tagcaaggaa aaagaagatt tcccaaggct
     3121 agtccagcat gtctttacca ttaaagataa gacagaccta gtcatttcag gcctaggctg
     3181 gattcgtgta acaggcacag caaaagtcgc cgtctgggca ccagaaggcg tcgccgtcgt
     3241 cacacgaaaa gcaattattt aagcacagaa aggaaagggt tgtctgaatt tgggcgagca
     3301 aggcgagccc catagagaat acttttcgct gtggtgtaag ttggtacaag tgattgtacc
     3361 aactgcggaa aatttgagac cttaggctca aattttagtc atgaaagtcc gaaggacttt
     3421 gctgacgtcc gtcaccactt cagaaaagta taaaaagaaa ctcttttaaa gaaattatgt
     3481 cattaacatc aaaacaacgt gccttcctca acagccaggc acacaccctc aaacctatca
     3541 tccaaatcgg gaaaaatgga ctcaacgacc aaatcaaaac cagcgtccgt caagctcttg
     3601 atgcgcgtga attaatcaag gttactctct tacaaaacac agatgaaaac atccacgaag
     3661 tagctgaaat tttggaagaa gaaatcggtg tggatacagt ccaaaaaata ggacgcatct
     3721 tgattttgtt taaacaatct agcaagaaag aaaatcgcaa gatttctaag aaagtcaaag
     3781 aaatctaaga ccgaaactcc aaataactgt ttttatagag aaataaaggg gactagccta
     3841 tgacaatcga actattgact ccctttacca aggtagagtt ggagccagaa atcaaggaga
     3901 aaaaacgcaa acaagttggg attttagggg ggaattttaa ccctgttcac aatgcccatc
     3961 tcattgttgc ggatcaagta cggcaacagt tgggactgga tcaagttctt ctcatgcctg
     4021 aataccaacc tcctcacgtt gataaaaagg aaaccatccc tgaacaccat cgtctcaaga
     4081 tgcttgagtt ggcaattgag ggaattgacg gcctagtcat tgaaaccatt gagttggagc
     4141 gcaagggtat ttcctacacc tacgacacca tgaagatttt gacagagaag aatccagata
     4201 cggattatta ctttatcatc ggtgccgaca tggttgacta tctgcctaag tggtaccgaa
     4261 ttgatgaact ggttgacatg gttcagtttg tgggggttca gcgtccacgc tacaaggtag
     4321 ggacttccta tccagttatc tgggtggacg taccgctcat ggatatctcg tccagcatgg
     4381 tgcgggactt ccttgcccaa ggtcggaaac ccaactttct cctacctcag cctgtgctag
     4441 actatatcga gaaggagggg ctctactgat gtcctatcaa gactatatca actgctcccg
     4501 tgaggccttg ttggaaaaaa tggcagagct tctacctgag aaacgtctaa cccattgtct
     4561 aggtgtggag cgtgccgcta tggaattagc tcagcgcttt ggtgtagatg ttgagaaagc
     4621 aagtctagca ggtcttcttc atgactatgc taaaaagttg tcagatcagg aatttctagc
     4681 cttgattgac cgttaccagc tagaccctga cctcaaaaac tggggcaata atgtctggca
     4741 tggtatggtc ggaatttaca aaattcagga agatttggat ttgcatgatt ctgaaattct
     4801 gcgagccatt gaaattcata cagtcggagc tggtcaaatg acagacctag ataaggtcat
     4861 ctacgtcgca gactatatcg agcacaatcg ggcatttcct ggagtggatg tggcgcgtga
     4921 gattgcaagt ctatcgctca ataaggcggt ggcctacgaa acagctcgta ccgtggagta
     4981 tctagctcat cagggattcc ccatctatcc ccaaaccctt gaaacctata acgcctttgt
     5041 gcactatttg aaagaggact aaatgaagac ggcttttata atcattgatg ttcagaatgt
     5101 tttagtagaa actgggttcc agacaaaaag tctattggaa aaaatttctt atttacaaaa
     5161 ccaggctaga agcaagaata ttgaaattat ctatgttcaa catattgaga actctgaagc
     5221 tcaaacatca gaagattggc agttatctgc gcttttaaat cgaaaacctg ctgaaaaggt
     5281 ctttcagaag aagtataaca gtattttcaa agaaactggc ttaaaagaat acttggataa
     5341 acaggggatt gaaaaattag ttttatgtgg tatgcagaca gaatattgtg tggatacctc
     5401 tgtcaaggtt gcctttgaat atggctatca gcttattgtt ccagaaggtg ctgtcacaac
     5461 ctttgatggg gatgacattc cagcagaaac gattaatgaa ttttatgagg acatttggga
     5521 ggagcgcttt gcagatgtcc tagattacaa acatattttc taaaaagagg attaaatgaa
     5581 cgaaaaagaa ttactagaac tagtcgtgaa agcggctgat gagaaacgtg cggaggatat
     5641 cctcgcactt gatgtacaag atttgactag tgtgacggac tactttgtca tcactagctc
     5701 aatgaatagc cgtcagttgg acgctatcgc agctaatatc cgtgaaaaag tagctcaagc
     5761 aggctttaaa ggtagccatg tcgaaggcga tgcagctgga ggctgggtct tactggacct
     5821 cggtgctgtt gtcgtgcata tcttttcaga agaaatgcgt gcccattata atctagagaa
     5881 gctatggcat gaggcgaatt cagtagatat ttcagaagct cttgcttaga agatgaactc
     5941 agttgtagtc aactgggttt ctttgcttat tttagaaaaa ttgatagaaa ggtaaagggc
     6001 ctcgtgtttg tttcatttcg aaactgaaca cgagctaaaa gctgagataa aaagtaactt
     6061 tctttgtatc tgacgatagg gaagaaagtt tcctatgtac tctttgcttt taaggctctt
     6121 tgtatcttag aaaggatttg aacccgtcct gtaattctcg gaaaatagat agccttcctt
     6181 ggagctcggc tccatcgtaa ggctcctaat tttctatcga attacgggcg gacttggtat
     6241 catgttttat ggcgacttat gaaacctttg cggcggtcta tgatgcggtc atggacgata
     6301 gtttatacga caaatggacg aatttttctc tgcgtcattt gcctaagacc aaggagagaa
     6361 agaaactctt ggaattggct tgtgggacag gaattcaatc agtgcgcttc tctcaggctg
     6421 gttttgatgt gactggactt gacttgagtg cggatatgtt gaagattgcg gagaagagag
     6481 caacttcagc caagcaaaag attgctttta tagaaggtaa tatgctgaat ttgtccaagg
     6541 cagggaaata cgattttgtc acgtgttatt cggactctat ctgctatatg caggatgagg
     6601 tggaagtagg ggacgtcttt aaggacgtgt acaatgtgct gaatgaagag ggagttttca
     6661 tctttgacgt gcactcgacc taccagacag atgaagtatt ccctggctat tcctaccatg
     6721 aaaatgcgga ggatttttcc atgctttggg atacatatga ggacgaagct cctcactcca
     6781 tcgtgcatga gctgactttc tttatcaagg aggctgacgg ttcctttagt cgccacgatg
     6841 aagtacatga ggagaggacc tatgagatct tgacctatga tatcttgctg gaacaggctg
     6901 gattcaagtc cttcaaactc tatgcagact ttgaggataa ggagccaaca gaaactagca
     6961 cccgttggtt ttttgtggcg cagaagtaaa tcaaatattg ttaatcttat gcttaggagg
     7021 taagggaaaa tgaatatacc aataccattt ggtgatattc ttgaaaaaga agtaattacc
     7081 aatataccta aaatttatca aaagttaaag caaatttata acgacttaca gtttaaaaca
     7141 gaagaagagt taggtgttgt ttatcaagaa tatttacaat ttacttataa aaaat
//
LOCUS       DAVKHZ010000088         6822 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000088 DAVKHZ010000000
VERSION     DAVKHZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.088"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            87..3437
                     /locus_tag="VWW99_001862"
     CDS             87..3437
                     /locus_tag="VWW99_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9539660.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMINLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKIDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3943..4413)
                     /locus_tag="VWW99_001863"
     CDS             complement(3943..4413)
                     /locus_tag="VWW99_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9539661.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4430..6703)
                     /locus_tag="VWW99_001864"
     CDS             complement(4430..6703)
                     /locus_tag="VWW99_001864"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9539662.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2088 a         1147 c         1473 g         2114 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgaaaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tggagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttacatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgaattt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa cttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatggcttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttgttttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctctgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaatagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gtagatggaa
     3781 gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
     3841 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
     3901 accaggtttt ttaaaagcat aattgttagt tgtagtcatt tattattctt caaagaaaaa
     3961 tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg catcttcaca
     4021 gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga agctcaaagt
     4081 atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta ggacttggtg
     4141 ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac gtttttccca
     4201 ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc ggacttttga
     4261 taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa tgtcgttctc
     4321 agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga taagagcgcg
     4381 tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt tagcaaggta
     4441 aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg tcacggatgg
     4501 tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct tttagcttgt
     4561 aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta atatcctcta
     4621 ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg actcgttgtg
     4681 cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga ccgttgtcaa
     4741 tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc ccaaattcta
     4801 gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga ttgagctgag
     4861 cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc tgattggctt
     4921 tcccttgata gagctctgta ttgaaggttt ggtatgtctt accatagcgc tcaaaatcct
     4981 tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag gtttcacagt
     5041 tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta ttgtcctgcc
     5101 agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg gtcaataagg
     5161 catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca tgggcaccat
     5221 tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc tggtaaacat
     5281 gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct gcctttactt
     5341 tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc ccagtttttc
     5401 ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcattg ccacggatat
     5461 agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca aagtcctcac
     5521 ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg taccatgatg
     5581 aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc gcaagaccat
     5641 tggacatggt acctagatag gaaagtcctg ttgtagcaac ttttccgttt gaccaatcag
     5701 ccttgacttg acgctggcgc gtgtgatcag taaaggcacg gcaacggccg ttaagccaat
     5761 cgatgacatt tttataagcc tcgatttgct ggtagtctcc attagtcatg aaacctgtcg
     5821 agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga aggaagtagt
     5881 cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct gctataagct
     5941 cagctttacc ttggggttgg acgagattta gttgaggttt ctctagctca atcttgtgag
     6001 gaagtttaac ctcaagctcg ccctccatct tgtagagagc cttgtcacta gccttgtcat
     6061 tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca tcaaaacgag
     6121 ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta tcgacacgag
     6181 actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc aaactcttgc
     6241 cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca agttggtcga
     6301 taagagtatt tccttttttg gtgcgagtat tgagtaactg atagagattt tcaatcaagt
     6361 caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct tcgaagtcaa
     6421 ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct gctgtcagtt
     6481 catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct aggatagaaa
     6541 gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt tcaagtgagt
     6601 ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagttca gataagacat
     6661 tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct ccatatagaa
     6721 tgacagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa cttcaaaagg
     6781 aaataatcca ataaaaatga ataaagtact aaattcaata ta
//
LOCUS       DAVKHZ010000089         6708 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000089 DAVKHZ010000000
VERSION     DAVKHZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.089"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            125..988
                     /locus_tag="VWW99_001865"
     CDS             125..988
                     /locus_tag="VWW99_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539663.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1371..1991
                     /locus_tag="VWW99_001866"
     CDS             1371..1991
                     /locus_tag="VWW99_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539664.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2077..2520
                     /gene="tsaE"
                     /locus_tag="VWW99_001867"
     CDS             2077..2520
                     /gene="tsaE"
                     /locus_tag="VWW99_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9539665.1"
                     /translation="MYIKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLSEELQYGV"
     gene            2510..3028
                     /locus_tag="VWW99_001868"
     CDS             2510..3028
                     /locus_tag="VWW99_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539666.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            3036..4052
                     /locus_tag="VWW99_001869"
     CDS             3036..4052
                     /locus_tag="VWW99_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9539667.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4137..5393
                     /locus_tag="VWW99_001870"
     CDS             4137..5393
                     /locus_tag="VWW99_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9539668.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKTSSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5448..6614
                     /gene="recA"
                     /locus_tag="VWW99_001871"
     CDS             5448..6614
                     /gene="recA"
                     /locus_tag="VWW99_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9539669.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         2133 a         1084 c         1502 g         1989 t
ORIGIN      
        1 ttttatgtaa tttttgactt tttttagttt attaccataa agaattattt atttctttaa
       61 cttgctattt tgaactaaaa attttataat gcaattaagc aaataggaag gtttattatc
      121 tttaatgagt acactcgcag agaaattcag attgaaaaga aaagagttga gactctccca
      181 acaaactctt gcagaaggaa tttgtgaaca aagccagatt agtaaaattg agagagggca
      241 tttcattccc tccgcagacc ttttgttcaa actctctcaa cgacttgaag taccattaga
      301 ttattttttt aatgaacaaa ttgaaattaa atctaacctc tctaatttca agcaattgtc
      361 tgctcgacta ttagacgata gaaattatga cgatttggaa tatatttata gaatagagat
      421 tgaacgaagt acttttctaa cactagaaga ccgaacttac cttgaatgga ttaaagctat
      481 tattgacttc tatcaatatg acagtaagtg tgaggcaatt tcttcattgg aaaatatatt
      541 attaaaagtc tcttcaaata ctctgattta tttaaaggcg ttgaatactc tatctaattt
      601 ctattcctta gtgggtcgtg aacaagaata tgaggcaaac tactctcatt taatagagtt
      661 gtatcagaca aaaaatttgg accatcaaga gtttttattt ggctacatca gagttcgtta
      721 caactacgct cactacctag tatcaaagga aaaatataat gaagccatcc aagaagctct
      781 tgaaacgatt gaactctgta aacaaagaca gacaagctac caattggctc ctctacttat
      841 tcttgtagga aatgctggag ccaaatttct agacagagaa caagtcaaaa attattatat
      901 agaagcaaga gagttatgta agatttataa caatccttta atgttgatga agatagaaaa
      961 ttatttgaag gaactagata ctgtttagtt aatcttgaca ttatagtttt tctgaaacgt
     1021 taaataacct gtaaggctga tagttagatt aatactatca gccttataat tatgtgactc
     1081 tgagtccgtc tcgctccgtg aggtgcgaga caaaacaacc acccgtttga cggattttca
     1141 tgatttcaaa tttatattct tcgaaaatct cttcaaatca cgtcagctct atctgcaatc
     1201 tcaaaacagt gttttgagca acctgtggct agctacctag tttgatcttt gattttcatt
     1261 gagtattagt ctctagtgtt tgtttagcgt cttaaagaga catttgagaa gattagaaac
     1321 agttctctct tttctcctag tataatgatt ggtaggaagg gagagagaag atgaaatcaa
     1381 tgagaatctt atttttgtta gctttaattc aaatcagttt gagtagctgt ttcttatgga
     1441 aggaatgcat cttgtccttt aaacaaagta cagctttttt catcggaagc atggttttcg
     1501 tttcaggaat ctgtgctgga gtaaattatc tttatacccg taagcaagaa gtccatagtg
     1561 tcctagccag taagaagtcg gtgaagcttt tttacagtat gttactctta attaatttgt
     1621 taggagctgt tcttgttttg tcagataact tgttcatcaa aaatacgctg cagcaagaat
     1681 tagttgactt tttattgcca tccttctttt tcctatttgg gctagatttg ctgatttttt
     1741 tacccttgaa aaaatacgtg cgcgattttc ttgctatgct agacagaaaa aagacagtgt
     1801 tggtgactat tttagcaaca cttcttttct taagaaatcc aatgaccatt gtctcacttc
     1861 tgatttatat tggactgggc ttgttttttg cagcctatct tgtcccaaat tcggttaaga
     1921 aggaagtttc cttttatggt catattttcc gagatcttgt attggtcatt gttacgctca
     1981 ttttctttta gagaaagcct gttttttatg gatgttatga tgataagttt agtaggaaaa
     2041 tagacacaaa agaaaagttt ttggtataat aagagtatgt acataaaaaa tgaagaagag
     2101 ttgcaagcct taggggagcg tttgggccat ctattagcaa agaatgatgt tttaatctta
     2161 attggagaac tgggtgcagg taaaacgacc tttactaaag gacttgcaaa aggattacag
     2221 atttctcaaa tgattaaaag tcctacctat actatcgtga gagagtatga aggtcgactt
     2281 ccactttatc acctagatgt ttatcgtatt gaaggagatg ctgattctat cgacttggat
     2341 gagtttatct ttggtggcgg cgtgactgtt attgagtggg gaaatctctt aggagatgcc
     2401 ttgccagatg cttatttaga attggaaatt ctaaaagaag cagatggacg ccgtttaaat
     2461 tttcaggcaa agggtttgcg tgctgagaaa ttgtcagagg agcttcaata tggagtatga
     2521 attgctcatt agggaagcag agcccaaaga tgcagctgaa ttagtggcct ttttaaatcg
     2581 tgtgagtttg gagacagact ttaccagcct agacggagat ggtattctct tgactagtga
     2641 ggagatggaa atattcctca acaagcaagc tagttcggac aatcagataa ccttacttgc
     2701 attcttaaat ggcaaaattg ctggtattgt aaatattaca gctgatcagc gtaagagagt
     2761 ccgtcatatt ggagatctct tcattgtgat tggaaaaaga tattggaata atggcttggg
     2821 aagtttgttg ctagaagaag cgatagagtg ggcacaagca agtggcattc tgcgtcgtct
     2881 ccaactgact gtccaaactc gtaatcaagc agcagtccat ctttatcaaa agcatggctt
     2941 tgtcattgaa ggtagccaag agcgtggggc atatatagaa gaagggaaat ttatcgatgt
     3001 ttacttgatg ggtaaactga taggttagta gaaatatggt taaaaaaatt attggaatgg
     3061 tgctagcttt actttctgta actgtagtag gagtaggtgt ttttgcttat actatttatc
     3121 aacaagggac agaaacctta gctaaaacct ataaaaaaat cggcgaagaa accaaggtta
     3181 ttgaagcgac tgaacctcta accattctgt taatgggagt ggacaccgga aatgttgaac
     3241 gaactgaaac ttgggtcggt agaagtgata gcatgatctt gatgacagtg aatcctaaaa
     3301 cgaaaaaaac aacaatgatg agtttagagc gggatattct gacgcgcatt gaatcaggga
     3361 atggtcaggc tcatgaagcg aaactgaact cagcatatgc agatggtgga gcagagcttg
     3421 ctatagaaac cattcaaaaa atgatgaata tccatattga tcgctatgtg atggtcaata
     3481 tgagagggtt gcaaaaatta gtggatgcag taggaggtat tacagtcaat aatatcctag
     3541 gtttcccaat ttctatcagt gaccaagaag aatttaatac catttctatc ggtgttgggg
     3601 agcaacatat tgggggagaa gaagccctag tctatgcacg aatgcgttac caagatcctg
     3661 agggggatta tggtcgtcaa aaacgtcaac gtgaagttat tcaaaaagtc atggaaaaag
     3721 ctctcagttt aaatagcatt ggtcattatc aagagattct aaaagctttg agtgacaata
     3781 tgcagaccaa tattgatttg tctgcaaaaa gtatccctaa cttgctaggc tataaagatt
     3841 catttaaaac cattgaaact cagcagttgc agggtgaagg agagatactt caaggtgttt
     3901 cttaccagat tgtttcgaga gcacatatgt tggaaatgca aaatatactc cgacgttctt
     3961 tgggacaaga agaagttact cagcttgaaa ccaatgcggt tttatttgaa gatttatttg
     4021 gcagagcacc tgttggtgat gaagataatt aacttttgat acaaataaaa aaatcaatcg
     4081 taggaaaatt cctgcgattt tttcaaaaaa atacgaatag ataggtagga ggaaacatga
     4141 aagcagaaat cattgctgtt ggaacagaga ttttgacagg acagattgtc aacaccaatg
     4201 cccagttttt gtcggaaaaa ctagctgaga ttggggtaga tgtatatttt cagacggctg
     4261 taggagacaa tgaagttcgt ctcttgtctt tgcttgagat tgccagtcaa cgtagcagtc
     4321 tggtgatttt gacaggcggt ttggggccaa ctgaggacga cctaaccaaa caaaccctag
     4381 ctaaattttt agggaaagca ttagtctttg atcctcaggc tcaggagaag ttggatatct
     4441 tttttgccct gcgaccagac tatgcccgaa caccgaataa cgaaagacaa gctcaaattg
     4501 tagaaggagc gattccactg ccaaacgaaa caggactggc tgttggagga aaattagaag
     4561 tagacggagt gacctatgtc gtccttccag gtccgccaag tgaattgaaa cccatggtct
     4621 taaaccaact tctacccaag ttgatgacag ggagcaagct gtattcccga gttcttcgtt
     4681 tctttgggat tggcgagagc cagctggtta cgattttggc tgatttaatt gataatcaga
     4741 tcgatcctac cttggcccct tatgccaaga caggagaagt cactctacgt ctgtcaacaa
     4801 agactagcag tcaagaagag gcgaatcaag cgctggatat cttggaaaat caaatcttgg
     4861 actgccagac tttcgaagga atttctttac gagacttttg ctatggttat ggggaagaaa
     4921 ctagtttagc cagcattgtg gtagaagaac tgaaaaggca agggaaaacc atcgcggctg
     4981 cagagagttt gacggcaggt cttttccaag ctaccgtggc gaatttttct ggagtttcaa
     5041 gtatatttaa gggtggtttt gtgacctata gcttggagga aaaatcaagg atgttggata
     5101 ttcctgccaa gaatttggaa gaacatggtg tggtgtctga atttacagct cagaagatgg
     5161 ctgagcaggc acgaagcaag acccagtctg attttggaat tagtttgact ggagtggcag
     5221 gaccagatag cctagaagga cacccagttg ggacagtctt cataggcttg gcgcaagatc
     5281 aaggaactga ggttatcaag gttaatattg gaggcagaag ccgagcagat gtacgtcaca
     5341 ttgcggttat gcatgccttt aacctagttc gcaaggcttt attaagtgac taacttttga
     5401 tataatagta gataggtctg aggatcatta gaatgtagga gaatagaatg gcgaaaaaac
     5461 caaaaaaatt agaagaaatt tcaaaaaaat ttggggcaga acgtgaaaag gccttgaatg
     5521 acgctcttaa attgattgag aaagactttg gtaaaggatc aatcatgcgt ttgggtgaac
     5581 gtgcggagca aaaggtgcaa gtgatgagct caggttcttt agctcttgac attgcccttg
     5641 gctcaggtgg ttatcctaag ggacgtatca tcgaaatcta tggcccagag tcatctggta
     5701 agacaacggt tgcccttcat gcagttgcac aagcgcaaaa agaaggtggg attgctgcct
     5761 ttatcgatgc ggaacatgcc cttgatccag cttatgctgc ggcccttggt gtcaatattg
     5821 acgaattgct cttgtctcaa ccagactcag gagagcaagg tcttgagatt gcgggaaaat
     5881 tgattgactc aggtgcagtt gatcttgtcg tagtcgactc agttgctgcc cttgttcctc
     5941 gtgcggaaat tgatggagat atcggagata gccatgttgg tttgcaggct cgtatgatga
     6001 gccaggccat gcgtaaactt ggcgcctcta tcaataaaac caaaacaatt gccattttta
     6061 tcaaccaatt gcgtgaaaaa gttggagtga tgtttggaaa tccagaaaca acaccgggcg
     6121 gacgtgcttt gaaattctat gcttcagtcc gcttggatgt tcgtggtaat acacaaatta
     6181 agggaactgg tgaccaaaaa gaaaccaatg tcggtaaaga aactaagatt aaggttgtaa
     6241 aaaataaggt agctccaccg tttaaggaag ccgtagttga aattatgtac ggagaaggaa
     6301 tttctaagac tggtgagctt ttgaagattg caagcgattt ggatattatc aaaaaagcag
     6361 gggcttggta ttcttacaaa gatgaaaaaa ttgggcaagg ttctgagaat gctaagaaat
     6421 acttggcaga gcacccagaa atctttgatg aaattgataa gcaagtccgt tctaaatttg
     6481 gcttgattga tggagaagaa gtttcagaac aagatactga aaacaaaaaa gatgagccaa
     6541 agaaagaaga agcagtgaat gaagaagtta cgcttgactt aggcgatgaa cttgaaatcg
     6601 aaattgaaga ataagctgtt aaagcagtgg agaaatccgc tactttttcg atttttgatt
     6661 caagttttta gattatatat agtagcttga aataagatat gaacaact
//
LOCUS       DAVKHZ010000090         6398 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000090 DAVKHZ010000000
VERSION     DAVKHZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.090"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            132..923
                     /locus_tag="VWW99_001872"
     CDS             132..923
                     /locus_tag="VWW99_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539670.1"
                     /translation="MNFALNKGEIVGQVGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKEMLKDIYLNRSK"
     gene            939..1667
                     /locus_tag="VWW99_001873"
     CDS             939..1667
                     /locus_tag="VWW99_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539671.1"
                     /translation="MKLNKFNFIKENIRDLYSSGVIYLGLIISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRIISRDFSHNTISLFYNQQKNRMIYLLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVTSNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            2053..2262
                     /locus_tag="VWW99_001874"
     CDS             2053..2262
                     /locus_tag="VWW99_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539672.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2252..2740
                     /locus_tag="VWW99_001875"
     CDS             2252..2740
                     /locus_tag="VWW99_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539673.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            2856..4316
                     /locus_tag="VWW99_001876"
     CDS             2856..4316
                     /locus_tag="VWW99_001876"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9539674.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDGDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGNQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4313..5137
                     /gene="nadE"
                     /locus_tag="VWW99_001877"
     CDS             4313..5137
                     /gene="nadE"
                     /locus_tag="VWW99_001877"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9539675.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5487..6038
                     /locus_tag="VWW99_001878"
     CDS             5487..6038
                     /locus_tag="VWW99_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539676.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKVYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1995 a         1071 c         1353 g         1979 t
ORIGIN      
        1 tgttaaaaag aggcgcaact ttttaaaatt aatgagaatc aaagagaaaa ccaataatat
       61 taatggagga ataaaaaatg taagttataa atgtaagtaa gcattatggt cattcaatca
      121 ttctcaaaga cataaatttt gcacttaaca agggtgaaat tgttggtcaa gtagggagaa
      181 atggagttgg taagagtacg ttgatgaaaa ttcttgttca gaataatcaa ccgacttcag
      241 gtaatattat aagcagtgat aatgttgggt atttaatcga agaaccaaaa ttatttttat
      301 ctaaaacagg tttagagaat ttaaaatatt tgtcaaattt atatggtgtt gactacaatc
      361 aagaaagatt tagaagtttg atccaagagt tagatttgac tcagtctatt aataaaaaag
      421 taaagaccta ttctttgggt acaaaacaaa aattagcttt gcttctaact ctcgttacgg
      481 aacctgatat attgatttta gatgaaccga ctaatggttt agatattgaa tcatcacaaa
      541 tagttttatc ggttctaaaa aatttagctt tacatgaaaa tgtgggaatt ttaatatcga
      601 gtcataaatt agaagatatt gaagaaattt gtgagagggt tcttttcttg gagaacgggc
      661 ttttgacatt tcaaaaagta ggaaaagata gtcataattt cttgtttgag atagcttttt
      721 catcagctac agatagagac attttcatta ccaaacaaga atttggggat attgttcagg
      781 aagagggact gagaattact atgtctggga atattcaaag tagtgagctt tttaaatttt
      841 ttaacgaaaa ctctattaaa gtagttgatt ttgaaactaa aaaagagatg cttaaagata
      901 tttatctaaa tcgttcaaaa taaaggaagg ttataatcat gaaattaaat aaatttaatt
      961 ttattaagga aaatataagg gatttatatt catcaggcgt aatatatctc ggtttgatta
     1021 tctcgtttat accgccgata ttgcttacat tctttattct aaagactcaa gggacatcgc
     1081 ttggtattaa gcatatttca aacttttatg ctatgctcgg tatgttaatg gctgttatac
     1141 atgctaaccg aattattagc agggattttt cccacaatac gatcagttta ttttataatc
     1201 aacagaaaaa tcggatgatt tacctcttgt ctaattttct atatgccatc tcagtttcca
     1261 ttatttatgc tttgaatggc attgtgttac tagtcatagt aagtaaattg ggtattccag
     1321 gtgatttagg attagatttt atagtagcta ttgtagtcaa tacaattttg ttagtcctgt
     1381 tttattttct attatcttac attttctatt tatacaaatt gaaaagtggc ttggtatttg
     1441 gtattttagt agctttacta ctctttatcc ctaatatatt aaatacgatg atgatgaata
     1501 ctagtaatga tttgtttatc aaagcaattg aacttcttcc tttttattct ttacctgtat
     1561 ttgtgacttc aaatacgatg tctattagtc agtatcttgt ggtaatcact acaatcattt
     1621 tattgtactt tttcactctc aagaaaagca agaagtattc attttagttt catttagtat
     1681 tattttgcat acctaaaata caataaaaaa atcagtcatc ttggtatgct cctgctttca
     1741 ctattcaaca cgtttttgac ttatactagg ctcatttcca aaagcattat ataatagtga
     1801 tatgaaacca actaaactaa acaagaaata taagcaataa aaactcgttt aaaagattct
     1861 actaaagcta atactaaata aaaataaaag agtaaactag gaagtttatt tcaaacaacc
     1921 taaaatactg attttcggct gaagataata ctggagtgca aattaatggg gttataataa
     1981 atagctgata gcttgtgttg gttttggatt ttttaagagt agatgagtat taaaactata
     2041 aggaggacga aggtggctaa aaatttaaaa ttaaaattag ctcgggtaga gcgtgattta
     2101 acacaaggtc aactggcaga ggctgtcggg gtgacacgcc agactattgg tttgatagag
     2161 gcgggaaaat acaatcccag tctctcgctc tgccagtcta tttgcagatg tttagggaaa
     2221 accctagacc aactattttg ggaggaagaa gatgaaaaat agattttatt attatcaatt
     2281 actagacgaa agagaagaac aactgttcaa taaagcgggc tctgaaagtt tctatatctg
     2341 cattgctttg tcgctcctat cttatatcat ttcagtatta gcaccaagcc tttttaattc
     2401 taatatgctg ctaatcgtta tcatcatagg gacattttac tttttcaatc gtgcccgtta
     2461 tctgggagtg acctactata gtcgttttca ttttacgatt ttgggttgtt ttttcctaac
     2521 cttggctatt acggctcttt tgatgttgca gaattatcaa ttcaacatag aaatttatca
     2581 gcacaatcct ttgaatttta aatacctgtc tgcttgggtc attacttatg tcatttacct
     2641 tccgtgggtc tttattggca atcttggtct taagagctat ggcgaatggg ctcagaaaaa
     2701 atttgaacaa gatatggatg aattggagag tggagaatag cttgttactc ttttctcaat
     2761 ccagctaaaa tgtgatataa tagtactaat ttattggaat acatgaaagt tcttgaaaat
     2821 tttcatgggt ttctagctaa ggaagtagga aaagtatgta tccagatgat agtttgacat
     2881 tgcacacgga cttgtaccag atcaacatga tgcaggttta ctttgaccaa gggattcaca
     2941 ataagaaggc ggtctttgag gtgtatttcc gccaacagcc ttttaagaac ggctatgcgg
     3001 tttttgcagg tttggaaaga attgtgaatt atcttgaaga cttgcgtttt tcagatggtg
     3061 atatagccta tttggagtcg cttggttatc atggggcgtt cttggattac cttcgcaatt
     3121 tcaagttgga gttgaccgtt cgttctgccc aagaagggga tttggttttt gctaatgaac
     3181 cgattgtgca ggtggaagga cctctagccc aatgtcagtt ggtcgaaacg gctcttttga
     3241 acatcgtcaa ctaccagacc ttggtggcga cgaaggcagc tcgtattcgt tcggttatcg
     3301 aagatgaacc cttgatggag tttgggacac gtcgggctca agaaatggat gcggccatct
     3361 ggggaacacg cgcagctgtg attggtggcg ccaatggaac cagcaatgtg cgtgcgggta
     3421 agctctttga cattcctgtt ttgggaaccc atgcccatgc cttggtacag gtttatggca
     3481 atgactatga agctttcaag gcttacgctg cgacccacaa aaattgtgtc tttcttgtgg
     3541 atacctatga cacccttcgc atcggtgtac cagctgccat tcaggtggcg cgtgagctgg
     3601 gtaatcagat taactttatg ggtgtgcgga ttgactctgg ggatattgcc tacatttcta
     3661 agaaagtccg tcagcaactg gatgaggctg gatttacaga ggctaagatt tatgcttcta
     3721 atgatttgga cgaaaatact atcctcaacc tcaagatgca aaaggccaag attgatgtct
     3781 ggggcgtggg taccaagctg attacagcct atgaccagcc agctcttggg gcggtttaca
     3841 agattgttgc aatcgaagat gaaactggtc agatgcgcaa tacgattaag ctgtctaata
     3901 atgcggaaaa agtgtcgacg ccaggtaaga agcaggtgtg gcgcattacc agtcgtgaaa
     3961 aaggcaagtc agaaggtgac tacatcactt atgatggtgt ggatattagc gacatgacag
     4021 aaatcaagat gttccatccg acctatacat acatcaagaa gacggttcgt aattttgatg
     4081 ccgttcctct cttggtggat atcttcaaag aaggaatatt agtttacaac ttgcctagtt
     4141 tgactgacat tcaggattat gcccgtaaag aatttgacaa gttgtgggat gagtataagc
     4201 gtgtgctcaa tccgcagcac tatccagtgg atttggcgcg tgatgtatgg caagataaga
     4261 tggacttgat tgataagatg cgcaaggaag cccttggtga aggagaagaa gaatgagttt
     4321 gcaagaaacg attatccaag agctgggtgt caaaccagtg attgatgccc aggaagaaat
     4381 ccgtcgttct attgatttct taaaaagata tctgaaaaaa catcccttcc taaaaacctt
     4441 tgtactaggg atttctgggg gacaagactc aaccttggca gggcgtttgg cgcaattagc
     4501 tatggaagaa ctgcgagctg aaacgggaga cgacagctac aaatttatcg ctgtccgcct
     4561 gccatacgga gtgcaagctg atgaagcaga tgctcaaaaa gccctagcct tcatccagcc
     4621 agatgtcagc ttggttgtga atatcaagga atcagctgat gccatgacag ctgcagttga
     4681 agcgacaggt agtcctgttt cagacttcaa caaggggaat atcaaggcac gttgccgtat
     4741 gattgctcag tatgcccttg ctggttccca tagcggagcg gtcattggaa cagaccacgc
     4801 cgcggaaaat atcacaggtt tctttaccaa gtttggtgac ggcggtgcgg atattctccc
     4861 tctttaccgc ctcaataaac gccaaggaaa acagctcttg cagaaacttg gcgcagagcc
     4921 agccctttat gaaaaaatcc caacggcaga cctagaagaa gataaaccag gcctagctga
     4981 cgaagtcgca cttggagtca cctacgcaga gattgacgac tacctagaag gcaaaacaat
     5041 cagcccagaa gctcaagcga ccattgaaaa ctggtggcac aaaggccaac acaaacgcca
     5101 cttacccatc accgtatttg atgacttttg ggagtaaaaa ggtccggggg acctttttag
     5161 cttcttgccc tgaaattaaa aagcaagaaa aacctccact ggaggttttc agcctctcat
     5221 cttgaaataa gaaagtgaga gaaggtctgg gggatcttga accccgagtt tagaaataag
     5281 aaaatgaggc agattcggta actcgaagag ttcgatttca tcgtcttacc cctgcaacga
     5341 tgactaggtt tgaaaaagat tgctagagcg catttcaaac caggcagcga ctgcgtcaag
     5401 aaattagaag acaaactcgt tttctagctg ttactgagtt gagtcttttt actacgagca
     5461 tagaaataag gaagtgaggt agcatcatga aatctatcgg tacgcaaata ttacagacag
     5521 aacgtttgat tttaagaaga tttgtggaga gtgatgcaga agctatgttt caaaattggg
     5581 cttcatccgc tgagaatttg acctatgtta cctgggatcc ccatcctgat gtcgaaatca
     5641 ctcgaaactc gattcgcaat tgggttgctt cctatactaa tctcaactat tacaaatggg
     5701 ccatttgtct aaaagaaaac ccagagcaag tgataggaga tatcagcatc gttaagatag
     5761 acgaggctga tttaagctgt gaaatcggct atgtgctagg caaggtttac tggggacatg
     5821 gtatgatgac agaggccttg aaagctgtct tggacttttg ttttactcaa gcaggttttc
     5881 aaaaggtcag agcacgttat gccagtctca atccagcttc aggtcgtgtc atggaaaagg
     5941 ctggaatgtc ctatctacaa accattgtta atggtgtaga gagaaaaggc tatcttgcgg
     6001 atcttattta ttatggtata agtagggaag aatgttgaat tctattttct gtttctatcg
     6061 aagtcaacta tttattgtaa atataataat tagcattcca agtttatttg aaactttaaa
     6121 atagcatatt gattagtaca agacagatgt tctagttcct tctttaatct ggtttagtat
     6181 tagttaaaaa atcgctttaa gcttgtaact aagagggagc taatcgacta gattctccag
     6241 ccgaacaggt ggtaatgtac tttttatagt gtaatcctag ctgttgttaa atttaaaata
     6301 gaatcctcta tcgagttagg gaattaaatt caaccaattt tattcatgtt ttttctatca
     6361 aattatctaa tattaaaata gtctcattct gatgagaa
//
LOCUS       DAVKHZ010000091         6117 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000091 DAVKHZ010000000
VERSION     DAVKHZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.091"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..482
                     /locus_tag="VWW99_001879"
     CDS             <1..482
                     /locus_tag="VWW99_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539677.1"
                     /translation="LYGSSLISFLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLC
                     FSLIHAQESTNQANLIGLQHQVPWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVA
                     LTSVLFALAHHPGTIIAWCLYVSLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            684..821
                     /locus_tag="VWW99_001880"
     CDS             684..821
                     /locus_tag="VWW99_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEU9539678.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            1000..3609
                     /locus_tag="VWW99_001881"
     CDS             1000..3609
                     /locus_tag="VWW99_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9539679.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSERLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGGIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            3642..5582
                     /locus_tag="VWW99_001882"
     CDS             3642..5582
                     /locus_tag="VWW99_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU9539680.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            5579..>6117
                     /locus_tag="VWW99_001883"
     CDS             5579..>6117
                     /locus_tag="VWW99_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539681.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSN"
BASE COUNT         2077 a          766 c         1218 g         2056 t
ORIGIN      
        1 ttctctatgg ttcgtcactc atttcttttt tatggatgta tggccaacgc aaacaagctc
       61 ataaggtaaa tatgaaatct aggatgaaat ggcttggtat tggattcgtt agcctactga
      121 ttataagtct atgttttagt ctaatccatg cgcaagaaag cacgaatcaa gcaaacttaa
      181 ttgggcttca acatcaagtt ccttggtttt catttttatt gtttttaatc aatgcgagta
      241 tggttgaaga atttctgtat agagaaattt tatggaactt ggttagaaaa ttagatattc
      301 gagttgcgtt gacaagtgtt ttatttgcct tagcacatca tccaggaacc attatagctt
      361 ggtgtttgta tgtatcgctg gggatgtttt tagggcttgt acgctacaaa ttggacttat
      421 ggggcagtat ggggctacat ttggtgtgga acctactagt ttatagtttg ctgctttttt
      481 aaaataggtg acctgatttt taggtaactt ctaaaactaa cttccagttt tgggagagag
      541 atggaagtta ctttgaaaag ttacggaaac attattttct atgtgacaac attgtataat
      601 tgcttgcatt ttactggtgg agagagtata atgagggtgg ataaagtact taatcccaaa
      661 aatttaaata ggaggagcta gctatggcag aagaagtatt aaatcttcaa ttagtatctg
      721 ttcaagttga cgaaacagat gaagttgatg gaatgcgatt tagtacattc agtacaaatc
      781 gttgcggtaa ctggagtgct tttagttggg aaaattgtta attcatttaa attgtaaact
      841 ttagagattc tctggagtta tggatgtcgt tccaatatgt gcacgttgga atgttagtgc
      901 ttatatgggg agaatccaaa ggattctccc tttttattta aataagtctg tctagctata
      961 gatatattaa gtttgataaa atttcttgga gattttgtta tggattataa tttcaatttg
     1021 gagcatcctt tctttttcac taataatgat tatagtacag atactagcat aaagtaccaa
     1081 gtaagtctgc cttttaattg gcacgaagta atgaataatg atgagtgggt ttatcaatac
     1141 cctattggaa aatttgttga aagacaaggg tggaaaatac acattagttc agagtataac
     1201 agttcacatg aattgttaca agatgtagca aaaatttgcc atgaaatgcg aattccattt
     1261 aaacatctta gtacagaaga caaatttatc atgcgaaacg ggaaattggt tagtcgagga
     1321 ttttctggta aatttattac atgttatcca aatcaaaatg aattagagtc ggttttgcaa
     1381 aggttagaaa gtgctttaaa gcaatataat gggccatata ttttaagcga taaacgttgg
     1441 gatgaagcac caatatatct gaggtatggt gtgtttaggc cgtcaaggga tgatgaaaag
     1501 aaggttgcta ttgatgaatt aattgttggc gatgaagttg ttaaagatga acgtttacca
     1561 gtatttaaaa ttccaaaagg tatagtgccc cctgactttt tgaataaatg gcttgacaag
     1621 aaagataaaa aacagggaga ctttcccttt attatagata atgcaattag attttcaaac
     1681 agtggtggga tatataatgc taggctaaaa gaagatggga aaaaaataat tttaaaagag
     1741 gctagaccgt atacaggtct aggatttgat ggtacttatt cttcagagag attagcttcg
     1801 gagtgcaaag cactaaaaat tctaaatgaa tggagtgaga cgcctaaaat atactggcac
     1861 ggtaagatct gggagcacac cttcttagga attgaacata tgaagggcgt tccactaaat
     1921 agatgggtta ccaataattt tcctttatat gaagttgttg ataagaccaa agattatcta
     1981 ttgagagttt caaagatagt agaaaaactc atcgatttaa cgaataagtt tcattctgaa
     2041 aatgtatatc atcaagattt acatttagga aatatcttgg ttaaggatga agatgagatt
     2101 agtataatag actgggaaca ggctgtcttt tcaaatgatg aaaaagttgt acataaagtt
     2161 gcagcccctg gttttagagc atggcgagaa acacttccca gtgagataga ttggtatggt
     2221 attagacaaa tagctcatta tttatacatg ccactagtaa ctacatcgga tttgacatac
     2281 aactatgtaa gtcaaaccag gatagaagga aaaaaactat ttgagtcttt gggttataca
     2341 agagaacata tagattatgt agaaagttta ttgtcatatc tagattctaa atgtccacag
     2401 attgagaata tttccaggaa aaaagtttta aaacccatgc atgagattag aactatcgag
     2461 agtgagcaag atatacaaga ttttattata aaattattga gagggtttac gctaacttat
     2521 ggacagtggc gtaaagaatt tcaatcaaga ttcttcccag ttcattatta tggtctgaat
     2581 tttaatcaag gaattgcgtt ttcagattta gctatccttt ggagttatca acaactagct
     2641 aagaaagtaa aaaatttcaa gtttgatgac tattatgaga ttcgaacaca ggttattaat
     2701 gaggcagtaa ataattttaa gaaatcgtcc ctttctggtc tttttgatgg taaaattggt
     2761 acaatctggc tcatttatga atttggggag atagatcggg cagttgaact ttttacaact
     2821 cattttatag agatatttga gaattcacaa aataaaaatt tatatagtgg gcaggctgga
     2881 attttactgg ttggactgta ttttcttagt aaagggggaa ttgataataa actaggagaa
     2941 gaaatactaa ttaggcttag agaatatact ttaaattata tagaaaatcc agaaactttt
     3001 tgtaaagttg gcgctagtga tgttcagagt aacgatcctt atgagaattt tggtggatta
     3061 ctatatggtc acgcaggggt agcttggctc ttcggtgaag cttataaatt aactggtgaa
     3121 agtatctata aaaatggtct agaattggct gtagataaag aattagttgc ttataaagta
     3181 gatagtaata attcattgca gtacagccaa ggtcatagat tactaccata tctagcaact
     3241 ggaagtgctg gcctgctttt acttattaat agaaataaag aaatcttgtc aagcaaatac
     3301 ttaaaatatt taacttcttt agagagggca actgatgtag ttttttgtgt attaccaggt
     3361 ttatttaacg gattttgtgg tcttgaagtt gctaataata tatatagtga tatcgatgat
     3421 aacttttcag gacaaaagaa attaattgaa caactttata gatatttgtg tgtaattgaa
     3481 gaaggatttg ttattgcggg agataatggt ttaaagataa caacagatat tgcttcggga
     3541 tttgcgggtg ttgcaattgg attggtatca ataatggata ataaattgac gattcttcct
     3601 caaatataat atttagatac cattattaag gggtcatact tgtgaatcag agtaatgaaa
     3661 tatttttaaa tacaatacag gaaaaaacac actataagag gtctatctct tcatcatttc
     3721 aggaattaaa aaaatggaat tctttgatat ttaggagagg gcgatattgg taccaatttg
     3781 ataattggag tatacccaat actggtgtaa taaaggaatg gtatgtcgaa gataatgact
     3841 ggattcctaa ggttagttct tccagtttag acaattcaaa ttcacttaaa attgtctttg
     3901 atgtggaaaa atatactttg ccagatgata gtatcttaca ggatgttagg atttcatctg
     3961 atgagagtta tatagttttg gttgtatcct cactgcgtag cactaattta attggaataa
     4021 aaaaatatac tatggaagag ttatttatca ttgaagatat ttcagtagaa agttcgttct
     4081 atttgggtaa attcggtgtt atgtatactc gctccaaaga gtacgggagg ccaagtaagt
     4141 tattctataa atcttttgat agctttacag aagaggaact gtttgaagaa aacgaatgct
     4201 cttttcgatt aaaaatagtc cacattgata gcaataattg ttttgtaaag tcagtagact
     4261 ttcagaaagg caggattttt ctatacagtt tcgaccggac gggttttgtt agacactctt
     4321 acacagaaac ggtagcccca acacctagag atatcgctct atttagtaca gaaaaagcac
     4381 agtactttct tgggctctct tcaactgaag aaaagaaaga tcaactaatt ctaagggaaa
     4441 cttcttcggg agacagagtt tctattacta taccatatca agatagggct agaagagttc
     4501 actgtatagg acgttatatc cttttagact gttcaaacgc agcgaattcc gtcttttatc
     4561 ttgctacatt taaaaataat agtttgaggg agttgagtgt taataagata acatttgatg
     4621 aaaagacaac actttatgaa aattcatttt ctgatcgtgt tcttctgttt ttagagagga
     4681 gaacttttta cgaaaaaata cttagttttg atttaataga aaatacatta aaaactgaat
     4741 ttgaaagacc catcataaaa tcgaataata ctaaatattt ttctaaggtg atttggacta
     4801 aaaaagacag ttatgatgta agcataccga tttctttatt ttggaaaagt gaagatacgg
     4861 atgagctccc aagaagaaaa aaatgtattc taagtgtcta tggggcttat ggtaagaatg
     4921 ataattctga tttagatgaa attatgttat ctataataga tgcaggtttt atttatgcta
     4981 tagttcatgt aagaggtgga ggctatttgg gtggtgaatg gtatcgctca ggaaaggcgt
     5041 taaacaaatg gaattctatc agagatttta ttgagggagt aaattattta agggaaaatg
     5101 atgtaattga cagtaagcga ttaggtttaa taacttctag tgcaggtgga ataattgctg
     5161 gtgcggtgtt gaacgaggaa aaaaatttat tgcaaagcat tctcttattt tcgccattta
     5221 taaatcctta tgatacactt cagaatccaa atgatcctct ttctaagacc gaaatagcag
     5281 agtggggaga tattagagac cctgaagtaa aggcttatat aaagtcatat tcgcctatgc
     5341 aaaatattga aaaggcacga gactcaaata ctgttatagt taatattttg ggtgagaaag
     5401 acccatatat taataataac gaagtgatag agtggtcaaa gaaattaaat tctattggag
     5461 ttaaaagttt gttgtattta aacaaagcag ctggtcatgg aggttttact ccatcagatg
     5521 ttctcttaat gattgatact ttaaattatt tctttgaaga agtgggaagg aataacttat
     5581 gaattattta aaatttataa aaaagactaa acttatatta atggggattt taatatttct
     5641 atcttcgttt aatggtgtct tgctctcagg aattattgta tatgcaggta gtttaaatca
     5701 gacttcctca ttttctgacg ttctaagatt tggtgctata agtattctgg gttggtcagc
     5761 aatctatata tcaaattact atttagaagt aacggaagca tcaataacta aagatataaa
     5821 tgtaaaaatt aaacaaggat attttagaga acagtatctt tcttctgaaa tggttaaaga
     5881 ttactcatct attatttcag ttttgtcaaa tgatttgaga ttaatcgaag aaaactattt
     5941 tagacaaatt tttgaaataa tttcttcaat attgttgttc atcgtctctc taagttttat
     6001 gctttattta aacttcctag tttcaataat atttattgta ttatcagcat tgccgattat
     6061 agtacctgtc tttatgaaga agatgttgtc taattcagct aatgagtact ctaacag
//
LOCUS       DAVKHZ010000092         6064 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000092 DAVKHZ010000000
VERSION     DAVKHZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.092"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..291
                     /locus_tag="VWW99_001884"
     CDS             <1..291
                     /locus_tag="VWW99_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9539682.1"
                     /translation="TLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERG
                     SNENHNRLIRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            472..1479
                     /gene="gap"
                     /locus_tag="VWW99_001885"
     CDS             472..1479
                     /gene="gap"
                     /locus_tag="VWW99_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9539683.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(1547..2383)
                     /locus_tag="VWW99_001886"
     CDS             complement(1547..2383)
                     /locus_tag="VWW99_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9539684.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            2509..4704
                     /locus_tag="VWW99_001887"
     CDS             2509..4704
                     /locus_tag="VWW99_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9539685.1"
                     /translation="MKLDELFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            4757..4909
                     /gene="rpmG"
                     /locus_tag="VWW99_001888"
     CDS             4757..4909
                     /gene="rpmG"
                     /locus_tag="VWW99_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9539686.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            4919..5095
                     /gene="secE"
                     /locus_tag="VWW99_001889"
     CDS             4919..5095
                     /gene="secE"
                     /locus_tag="VWW99_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9539687.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5150..5686
                     /gene="nusG"
                     /locus_tag="VWW99_001890"
     CDS             5150..5686
                     /gene="nusG"
                     /locus_tag="VWW99_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9539688.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            5886..>6064
                     /locus_tag="VWW99_001891"
     CDS             5886..>6064
                     /locus_tag="VWW99_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9539689.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYF"
BASE COUNT         1903 a         1050 c         1330 g         1781 t
ORIGIN      
        1 acgttactat taggggagca tcgtattctg tccattactg cagataatgg ttcggagttc
       61 aaacgattgt ctgaggtatt tcctgaggaa catatctact acgcacatgc ttactcttca
      121 tgggagagag gttcaaatga aaatcataat cgattaattc ggagatggtt acctaaagga
      181 accaagaaaa cgactccgaa agaagtagct tttatcgaaa attggattaa caactaccct
      241 aaaaaatgct tggactacaa gtcgccaagt gaatttcttt tgggtggcta acttcaactt
      301 gaaatttggg tttacagtct taaaaagaaa ctaacataga aaaacaatgg ccatacttgc
      361 aattcaactc aaatagttgt ataataggtt tgttgaaagg tgatttgtag tgattcaagt
      421 tactcttttc acaataaaaa tttcatgatt ttcataagga ggaaatcact aatggtagtt
      481 aaagttggta ttaacggttt cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac
      541 gtagaaggtg ttgaagttac acgcatcaac gaccttacag atccagttat gcttgcacac
      601 ttgttgaaat acgacacaac tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt
      661 ggatttgaag ttaacggtaa attcatcaaa gtttctgctg aacgtgatcc agaacaaatc
      721 gactgggcta ctgacggtgt agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa
      781 gaagcagctg aaaaacacct taaaggtgga gctaaaaaag ttgttatcac tgctcctggt
      841 ggaaacgacg ttaaaacagt tgtattcaac actaaccacg acgttcttga cggtactgaa
      901 acagttatct caggtgcttc atgtactaca aactgcttgg ctccaatggc taaagctctt
      961 caagacaact ttggtgttgt tgaaggattg atgactacta tccacgctta cactggtgac
     1021 caaatgatcc ttgacggacc acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct
     1081 gcaaacatcg ttcctaactc aactggtgct gcaaaagcta tcggtcttgt aatcccagaa
     1141 ttgaatggta aacttgatgg atctgcacaa cgcgttccaa ctccaactgg atcagttact
     1201 gaattggtag cagttcttga aaagaacgtt actgttgatg aagtgaacgc agctatgaaa
     1261 gcagcttcaa acgaatcata cggttacaca gaagatccaa tcgtatcttc agatatcgta
     1321 ggtatgtctt acggttcatt gtttgacgca actcaaacta aagttcttga cgttgacggt
     1381 aaacaattgg ttaaagttgt atcatggtac gacaacgaaa tgtcatacac tgcacaactt
     1441 gttcgtactc ttgaatactt cgcaaagatt gctaaataat tcttgagttg atagaaagca
     1501 aggctttgtg gtcttgcttt tttatatgga aaaatggatg acacgatcat ccattctttt
     1561 ttaattcttt ttcaaatgta tttgaaaggg tagtgaaagt tagcctctct aaagtaagtg
     1621 ggtgggtaaa ggaaagtcgg aaggcatgaa gcataagccg gcttgtcttt gatttactat
     1681 tatagagagg gtctcccagg ataggaagat tatgatgcaa aaggtgcaca cgaatctgat
     1741 gggttcgccc tgtctttagc ttgcaatgag ccaaggaagt cttgtttgag aattgcttta
     1801 atctgcttac atgcgtttca gcatattgcc cattttttgc atcaactatt ctttttctac
     1861 gatcatggcg atcacgtcca attttgtctc tgaaaacaag ttcttttctg ttgatatttc
     1921 catcaactag agcccaatat tctctagaaa tctctttttt ctccaataag cgattgagaa
     1981 tgggcaggat aaaaggattt ttggcaaaga gaactaagcc actggtttcc atgtccagac
     2041 gatgaacgac atagcaggtt tggccaacat aggtactgac atggttaaga agggcaattt
     2101 cgtttggttg attaccatgc gttttcatcc cctctggttt gtttacaata atcaagtgtt
     2161 gatcttgata aacttcctgc actaagtctg ggttgcccca agggatcgtc ttttgggaat
     2221 aatcttcctc gtcaaaagtc aactggcaaa catctccagg atttacgatt tcgttccagt
     2281 ggacttcttc ttgatttatc aaaatatgtt tcttgattct caaaaaatga cggatttttc
     2341 tagggatgag gagttgttcc tcaagtaatt gctttaccgt catttgaggt agagaggcgg
     2401 gtaatgtaaa tgtgaattgc atacagatat tgtaacaaaa aaagccctat ttggatagga
     2461 aatagctaaa ttcttgtctt cctatgatga agatgataaa ataaacgcat gaaattagat
     2521 gaattatttg agaaatttct ttctcttttt aaaaaagaaa caagtgaact agaggactct
     2581 gattctacta tcttacgtcg ctctcgtagt gatcgaaaaa aattagccca agtaggtccg
     2641 attcgaaaat tctggcgtcg ttatcatcta acaaagatta tccttatact aggtttgagt
     2701 gcaggattgc tagttggaat ctatttgttt gctgtagcca agtcgaccaa tgtcaatgat
     2761 ttgcaaaatg ccttgaaaac tcggactctt atttttgacc gtgaagaaaa agaggctggt
     2821 gccttgtctg gtcaaaaggg aacctatgtt gagctgactg acatcagtaa aaacttgcag
     2881 aatgctgtta ttgcgacaga agaccgttct ttctataaaa atgacgggat taactatggc
     2941 cgtttcttct tggctattgt cactgctgga cgttcaggtg gtggctctac cattacccaa
     3001 cagctggcta aaaacgccta tttatcgcag gatcaaactg ttgagagaaa agcgaaagaa
     3061 tttttccttg ccttagaatt aagcaaaaaa tatagtaagg agcaaattct aaccatgtac
     3121 cttaacaacg cttattttgg aaatggtgtg tggggtgtag aagatgcgag taagaaatac
     3181 tttggagttt ctgcatcaga agtgagtctg gatcaagctg cgactctggc agggatgctc
     3241 aaggggccgg aactgtataa tcccttgaat tccgtagaag attctactaa tcggcgcgat
     3301 actgtcttgc agaatatggt tgcagcagga tatattgata aaaaccaaga aaccgaagct
     3361 gctgaagttg atatgacttc gcaattgcac gataagtatg aaggaaaaat ctcagattac
     3421 cgttacccct cttattttga tgcggtggtt aatgaagctg tttccaagta taatctaaca
     3481 gaggaagaga ttgtcaataa tggctaccgc atttacacag agctggacca aaactaccaa
     3541 gcaaatatgc agattgttta tgaaaacaca tcgctatttc cgagggcaga ggatggaacg
     3601 tttgctcaat caggaagtgt agctctcgaa ccgaaaacag ggggagttcg tggagttgtc
     3661 ggtcaagttg ctgacaatga taaaactgga ttccggaatt tcaactatgc aacccaatca
     3721 aagcgtagtc ctggttctac aattaagcct ttagttgttt atacaccagc agttgaagca
     3781 ggctgggctt tgaataagca gttggataac cataccatgc agtatgatag ctataaggtt
     3841 gataactatg cagggatcaa aacaagtcga gaagttccta tgtatcaatc cttggcagaa
     3901 tcgcttaatc tacctgctgt tgccactgtt aatgatttgg gtgttgacaa ggcttttgag
     3961 gcaggcgaaa aattcggact caacatggaa aaggtcgacc gtgttcttgg tgtcgccttg
     4021 ggaagcggtg ttgaaaccaa ccctcttcaa atggctcaag catacgctgc ctttgcaaat
     4081 gaaggtttaa tgcctgaagc tcattttatt agtagaattg aaaatgctag tggacaagtt
     4141 attgcgagtc ataaaaattc acaaaaacgg gtgattgata agtctgtagc tgacaagatg
     4201 accagtatga tgttggggac tttcaccaac ggtaccggta ttagttcatc gcctgcagac
     4261 tatgtcatgg cagggaaaac tggaacaact gaagcagttt tcaatccgga gtacacaagt
     4321 gaccagtggg taattggtta tactccggat gtagtgatta gccactggct tggctttccg
     4381 accactgatg aaaatcacta tctagctggc tctacttcaa acggtgcagc tcatgtcttt
     4441 agaaacattg ccaataccat tttaccttat acgccaggaa gtacctttac ggttgaaaat
     4501 gcttataagc aaaatggaat tgcaccagcc aatacaaaaa gacaagtaca aaccaatgat
     4561 aatagccaga cagatgataa tttgtctgat attcgagggc gtgcgcaaag tctagtagat
     4621 gaggctagcc gggctatctc agatgcgaag attaaggaaa aggctcaaac aatatgggat
     4681 tcgatagtca atctatttcg ctaagatgct tgtcaaagcc tagctttctt gttataatgg
     4741 ataagatgga ggcgttatgg cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc
     4801 gagaaactat tcaatcaaga tcagcggaaa ccccaagcct acacgactag aagtaaataa
     4861 attttgtaag cattgtggca agtacactac acacagagaa acgagatagg agagagcgat
     4921 gcgttttatt ggagatattt ttagacttct taaagacaca acatggccaa ctcgcaagga
     4981 aagctggaga gattttcgtt ctatcatgga atacacagct ttctttgtag taattattta
     5041 catttttgac cagttgattg tttcaggttt gattcgattt attaacattt tttagaagat
     5101 tagtggagtt aattacacta gaaatcttct atttatgaaa ggaaatatca tggatagttt
     5161 tgataaagga tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa
     5221 tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc
     5281 aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt
     5341 tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa
     5401 cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt
     5461 attggaacaa gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga
     5521 tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac
     5581 tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa
     5641 tgacacagtt gcagaagtaa acctaaacca aatcgcagaa ttataaccct gagagaggct
     5701 ggagcctctc ttttttgtgc agtttaggag ctaaagggaa cagaatggag aaaatggaac
     5761 aaatgtgttt tctaatctgt tagactgtat ctagaaaggg gaaaattatg attaaagaat
     5821 tgtatgaaga agtccaaggg atgtgttttc taatctgtta gactgtatct agaaagggga
     5881 aaattatgat taaagaattg tatgaagaag tccaagggac tgtgtataag tgtagaaatg
     5941 aatattacct tcatttatgg gaattgtcgg attgggagca agaaggcatg ctctgcttac
     6001 atgaattgat tagtagagaa gaaggactgg tagacgatat tccacgttta aggaaatatt
     6061 tcaa
//
LOCUS       DAVKHZ010000093         5750 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000093 DAVKHZ010000000
VERSION     DAVKHZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.093"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(1..447)
                     /locus_tag="VWW99_001892"
                     /pseudo
     CDS             complement(1..447)
                     /locus_tag="VWW99_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(827..1687)
                     /locus_tag="VWW99_001893"
     CDS             complement(827..1687)
                     /locus_tag="VWW99_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9539690.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKSGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(1703..2980)
                     /locus_tag="VWW99_001894"
     CDS             complement(1703..2980)
                     /locus_tag="VWW99_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9539691.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(2973..3524)
                     /locus_tag="VWW99_001895"
     CDS             complement(2973..3524)
                     /locus_tag="VWW99_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9539692.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(3540..4799)
                     /locus_tag="VWW99_001896"
     CDS             complement(3540..4799)
                     /locus_tag="VWW99_001896"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9539693.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASIKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
BASE COUNT         1760 a         1300 c          930 g         1760 t
ORIGIN      
        1 ccttgacttg tgacaatcgc ttgagccttc atagcgtgaa atttcttttt accagaataa
       61 ttcgctaatt ctttttttag ggcgattggt ttttacttcc gtcgcatcaa tcattaccgt
      121 gtcctcagaa ctgagaggag ttcttgaaat cgtaaaacca ctttgaacaa gagttacttc
      181 aacccattgg ctccgacgga ttaagttgct ttcgtgaata ccaagatcag ccgcaatttg
      241 ttcataagtt cgatattctc gcacatattg aagagtggcc ataagaaggt cttctaggct
      301 taatttaggt tttcttccac cttttgcatg tttaagttga taagctgttt ttaatacagc
      361 taacatctct tcaaaagtgg tacgctgaac atcaacaaga cgcttaaatc gtgcatcagt
      421 tagttgttta cttgcttcat cattcataga actactatac catgttttgt ttcgcaggaa
      481 gtataatata aaaaaataaa tttaagattg gcaatacgaa cgttaaacag aattccattt
      541 ctctttttag aatcaaaacg acaatattgt gaaatctcta caggaacaat agactgcgac
      601 actaaaatat cctcccttag ttttacttta ctaatagtat ataggaggag agccctgaat
      661 cctagaccct ttgttatgat gcgcttacaa acagagttgg aaaaaatctt aaaagcagaa
      721 aaacgcatga tatcagcccc taacacttga tacaatacgt tttattatga aaagatctac
      781 tggatttttt atcaaaataa acttccagta gtcttttcac tttttattaa taagttcctt
      841 cttcaccttg gctagtcaag ataacaggtc catctttcgt aatgacaaat tggtgttcat
      901 actgacatga caatccaccg tcaatggtct tatgcgccca accagttttc atatctgtat
      961 caatttccca atcgcctgta ttgatcattg gttcaatggt taagaccatt ccttcacgaa
     1021 gacggagtcc acgacctgca ataccatagt taggaaccat tggttcttcg tgcatagttg
     1081 ggccaacacc atgaccaacc aaatcacgca ctacaccgta accacgactt tcagcgtatt
     1141 cttgaatagc cgcaccgata tcaccgatac gatttccaac aacagcttgc tcaataccct
     1201 tgtacatagc ttctttggtt acatccatca agtttttgac ttcttcggac ggtgtaccaa
     1261 cagcataagc ccaacatgag tctgctaaac caccagaata gctctgagta tattttttca
     1321 tttgttcaac attgttgaag tttaattttg agacattcag gtcagattta gcaatgggac
     1381 ctcccaaaac catatcaact ttgagcaaat caccatcttt caagatataa tgacgaggga
     1441 aagcgtgagc cacttcatcg ttaagagagc aacaggtagc ataaggatag tccatcatgg
     1501 caccgtcaac cccaatctga agtggaagga aattttcttc tttacaacga cggcggacat
     1561 attcttcaac ttcccacata tctacgcctg acttaatcaa atcacgtaag cctatatgaa
     1621 tacttgctag aaaatcacca gccttgtcca tagcttcgat ttcacgagct gattttaatg
     1681 ttatcatctt ttctcctagt ttctaattaa ttttaacagt cacatttgct tttgaaacaa
     1741 tctggtgacc atgataaata tcgtaatcaa ttatagctga ccgtctcgta tgatgaataa
     1801 tccgtgcctg aatgcgcaat atatcatcta tctgaacagc ctgcaaaaag tagatcagca
     1861 tctgctcgat aatgagattg cgaccactat taacaactaa atcttgggtc atgtgggtca
     1921 gaatttctgc caatacacca ttagccaaaa ctccattttt ttctagcata aagggttcca
     1981 ctgtaatgac tacttcatca tggtgataag agagcttttg tccaatctgc tcagaaaaag
     2041 ttggtagagc cgaaacttgg gaacggctca tcttctccat gacatctcgt cgcgtcacaa
     2101 cgccaagcaa agtttgattg cttcgaacaa ctggtaccat ttcaaagtct tctgcgatca
     2161 tccgttgact cacattggca atatttgtcg ataatccaac caaaaataga ctacgagaca
     2221 taaccttatc aattgtcgtg cttggtgatt tatcaccagc gtctctcatg gttacaacgc
     2281 caacaacgac ctgatgttga ttgataacag ggaaacggct gctacgattc ttacgaacca
     2341 agtccaaata atctttaact gtatctgtct ctctcagaaa accatactca tgactagggc
     2401 gataaagttt ctcaactgtc agaatatcag tcttgatttg gacatttgac aaggctttat
     2461 tgatcatggt cgcgacggta aaggtatcat gcttacttct tagaacagga atcccttttt
     2521 gattggccag tttaagcaca tcatcatgaa cctgaaatcc ccctgtaacc agaactgcat
     2581 tttcattttc caaggctagc aactgaatac gggttcggtc tccgacaatc aagagccccc
     2641 catcatgaag gtaagacaag atattttgtt cagtcatggc accaattgaa aacttactaa
     2701 attctctctc taaaccttct tgcccagcca gaacctcaga agaagtcact tctgcaattt
     2761 cagcaaacgt taatctctct atagcaactt tctgggattt aacacgaatt gttccacttc
     2821 taggacgggt ctccacaatt ccacggtttt cagcttcttt aatagcccga taggctgttc
     2881 catcactaac tcctagatga ttcgaaatgc tacgaacact gaccctttta cctactggta
     2941 attcctccaa atagcttaga atttcctgat gcttactcat tgaactctcc ttttacatac
     3001 cgaaattcaa gataatcccg catttttctt gtgtaacgat cacttccctt aaactgacgg
     3061 aacaaactaa atccagactt aagagcaact ctttggctag cttcattttc aaggtgggta
     3121 atgatagata attgttttaa gccaaattcc tcaaaagaaa gctgacaaat ttttctaaca
     3181 acctctgtca taaatccttg cgaccaagca tcttttctca aaaaatagcc aagctcagct
     3241 tcttttttga tttcatctaa cttctcaaat ttaatagaac caatcatttg ttgatttttc
     3301 tggtcacaaa ttgcccacac tcccaaaggg gacttcataa agtaattggc cagtgcatat
     3361 tgactttctt ccagacttgc ctgcgttggg aaaataaatt gaagattttc tggatttgaa
     3421 gctatctcgc ggaagtcctg actatcacta aaaaagaaag gacgcaaata caagcgatcc
     3481 gtttcaaaaa aagaaaacat tgctaatttg gtccaaatat tcataaacac ctctacggtt
     3541 taatcctcaa caagtctaat atccgctcct aaattacgta atttttcgat aatatcagaa
     3601 taaccacgta agataaactc gatattggta atttcagttt taccttcagc cataagccca
     3661 gcaatgacta gtgcagcccc agctcttaag tcggtcgctt taatactggc cccacgtaaa
     3721 tcacgtccac ccgtgtacaa aatatgacca tttgttgtcg aaatatccgc atccatcttt
     3781 gctagttcaa aaacatgatt tacacgtttt tcgtaaatcg tatcgacaat tgtaccacga
     3841 ccattcgctc ttagtaaaag aggggtaagc ggttgttgca aatcagttgc aaagcctggg
     3901 taaggagctg tcttaatatt gattgctttc aaattagact gttcctcgac aaaaatgctg
     3961 tcttcagata cagtcattct cactcccatt tcttccaact tagcaataaa cccttccagg
     4021 tgttcgtaaa gaacattatt tatacgaatt cctttaccaa ctgcagcagc taaagatata
     4081 tatgttccag cttcaatgcg gtctggaatc acctgatgac gtgtcccatg taatctttca
     4141 acaccatcaa taatgatgat attagttcct gccccacgga tatgggcacc catattattc
     4201 aagagagtag ctacatcaat aatctcaggt tcacgggctg cattttcaat aatagtacga
     4261 ccatttgctt taaccgcagc aatcatcgta ttaatcgttg ctcccacact aaccgtatcc
     4321 atgtaaatac ttgcaccatg aagtcctgta tctttagcag ataacttcat gttatctccc
     4381 tcgtagctag cagtggcacc catagcttca aacgccttaa ggtgtaagtc aatcggacga
     4441 ggaccaagat cacatcctcc cggtagacca actgtcgctt caccaaaacg gcctaagagg
     4501 ctcccataaa aatagtaaga tgcacgaaga ctgttaattt taccataagg cattggaata
     4561 ttttgaacac ctcttggatc aatctccaag acatcgtcat aacgcttaac agtagctccc
     4621 attaattcca tgatttcgac aagactggct acatccgaaa tatctggaac acaatccaaa
     4681 gtcaccacat catcagccaa tataatagct ggaattaagg ccacaacact atttttagca
     4741 ccactaatag taatttcacc ttgcagtggt aatccaccat tgataacaat ttttctcatt
     4801 ctaagttttc aatactcttt caagatttct aataaggtct tcaaaataaa gtatatcata
     4861 aatttatcct tttttccatc cttttcaatt ttattggata aatcagagtt gacgagttag
     4921 taactgaatt actaatactc aatgaaaatc aaagatcaaa ctaggaaact agccgcaggt
     4981 tgctcaaaac actgttttga ggttgtagat ggaactgacg tagtcagtat cctacacata
     5041 cggcaaggcg atattgacgt gatttgaaga gattttcgaa gagtataatc tttctcgaat
     5101 cgctattgcc ccttttttat cccctaaaac tgagtaggat ctaagactag atctcttatc
     5161 taagtatcgg aatacggctg ttcaactaaa ttttagcaag aacttctccc cttctctcag
     5221 ttcacagaag cgttttatac ataccgtcct atcagacaat ctattttaaa gataaccgtg
     5281 actcaggatc acttctatct gtcgttttct ctttatagtg atagacctca cgggataagt
     5341 cgtacatctt tcctcgtgta tacaagatat aaggacgtta aaaggttaca gtgaaaatag
     5401 aaaatcttag tacgaattca attactctcg tgatttacgc atataggtta cgactacctt
     5461 tttggacttt agagtttgag acccccttat ccgctatata cgccttacta tcacgttcct
     5521 gttcgtaaag ccacgatttc gctatccctt cctctgacga ttcttatttc cagcgtcaga
     5581 cttaaaacgg tctatcccca gaccgtttta atccactacc tcacgatagt cgggcttggg
     5641 aatcgctatt tgactcgccg agaactttga catcagatat actataacaa attgaaataa
     5701 gaatcaaaca atttaatttt gaaattcaaa tcaatttata acaatatagt
//
LOCUS       DAVKHZ010000094         5454 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000094 DAVKHZ010000000
VERSION     DAVKHZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.094"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            74..1828
                     /locus_tag="VWW99_001897"
     CDS             74..1828
                     /locus_tag="VWW99_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539694.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVAFANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1812..3533
                     /locus_tag="VWW99_001898"
     CDS             1812..3533
                     /locus_tag="VWW99_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539695.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3544..4128
                     /locus_tag="VWW99_001899"
     CDS             3544..4128
                     /locus_tag="VWW99_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9539696.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4128..4823
                     /locus_tag="VWW99_001900"
     CDS             4128..4823
                     /locus_tag="VWW99_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9539697.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFIA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            4811..>5454
                     /locus_tag="VWW99_001901"
     CDS             4811..>5454
                     /locus_tag="VWW99_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539698.1"
                     /translation="MVEIKNLSLDYGEERILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKG"
BASE COUNT         1950 a          715 c         1026 g         1763 t
ORIGIN      
        1 aaaagcaatg atagatatta tcaattaagc gaatttatat ctaaaaggga tattaaagaa
       61 aggagatatg cttatgaaga tttacaaaaa actatttgct tatgtccaag ataagaaata
      121 tcttggggtt ttggccataa ttttttctgc tatatctgct ctacttacag tatatggata
      181 ttatttaatc tacaaatttc tagataagtt aataattaat tcaaatttat ccggtgcaga
      241 gagtatagca ttaaaatctg ttattacact aacaagtgga gcgatatttt attttgtctc
      301 aggaatgttt tcacatatct tgggattcag gcttgaaaca aatttaagaa aaaggggaat
      361 cgatggtctg gaaaaagcaa gttttaggtt ctttgactta aatccatctg gtcaaataag
      421 aaagatcata gatgataatg cagcacaaac tcatcaggtg gtagcacaca tgattcccga
      481 tagttctcag gcaataatca cacccgtact tgtacttgca cttggcttta tagtaagtat
      541 aagagttggc ataattttgc ttgctcttac tataattggt ggcttaattt taggggcgat
      601 gatgggcgag caagaattta tgaagatata ccaagaatcc ctatctaaac taagtgctga
      661 aactgttgag tacgtgagag gaatgcaagt tgtaaaaata tttaaagcaa atgtagagtc
      721 atttaaaagc ttttacaagg ctataaaaga ttactcaaag tatgcttatg attattccct
      781 atcttgtaaa aggccttatg ttttgtatca atggttattt tttggactga ttgcaatttt
      841 aattattcct atagtttatt ttatgactag cttagctagc gcaaaggtga ttttacttga
      901 gcttatcatg attttatttt tatcaggagt tctctttgtt tcattcatga gaatgatgtg
      961 gtactccatg tatatttctc aaggaaatta tgcagtagat actttagagg cgctttacga
     1021 agatatgcaa aaagacaaat tagtgcatgg taatgtcaat aattttaaaa actataatat
     1081 agaatttgag aatgttagct ttgcttataa tgataaagct gtcattgaaa atttatcctt
     1141 taatttagaa gaaggaaagt cctacgcact tgtcggttca tctggatcag gcaaatcaac
     1201 agtagcaaaa cttatatcag gtttttataa tgttaataaa ggaagcataa agataggcgg
     1261 gatagcaata agtgaatatt ctgacgaagc cttaattaaa gccatttcct ttgtttttca
     1321 agattcaaaa ttattcaaga agagcattta tgataatgta gctttcgcta ataaagatgc
     1381 gacgaaagat gacgttatga gagccttaaa attagcagga tgcgatttaa tattagacaa
     1441 attcccagaa agagaaaata caatcatagg ctcaaaaggt gtttatttat ccggtggaga
     1501 aaaacaaaga attgcaattg ctagagcaat tttaaaggat tccaaaatta ttattatgga
     1561 tgaagcatca gcatctattg acccagataa cgagtttgaa ttgcaaaaag cttttaaaaa
     1621 tcttatgaag gataaaacag ttatcatgat tgcacacagg ctatctacaa ttaaagacct
     1681 tgatgaaatt attgtcatgg atagtggaaa aattatagaa agagggtctg acaaagaatt
     1741 aatgtcaaaa gatacaaggt ataagagcct gcaagagatg tttaacagtg cgaatgaatg
     1801 gagggtttca aatgaaagag ttttataaaa aaagatttgc tcttacagat ggaggagcaa
     1861 gaaatttaag taaagcaaca ctggcttcat ttttcgttta ttgtataaac atgcttcctg
     1921 ccatattact tatgattttt gctcaggaag ttttggaaaa tatgggcaaa agcaatggct
     1981 tttatatagt attctcagtt ttgactttga tagcaatgta tattttgctt tctatcgaat
     2041 acgataaatt atataacaca acctatcaag aaagtgcaga tttaagaata aggacagcgg
     2101 agaatttatc aaaattacct ctatcttact tttctaaaca tgacatttcc gacatttcac
     2161 aaacaatcat ggctgatatt gaaggcatag agcatgcaat gagccactca ataccaaagg
     2221 tgggcggcat ggtactgttt ttcccattaa tatctgtaat gatgctagcg ggcaatgtca
     2281 agatgggttt agctgtaatt attccatcta ttttaagctt tatatttata cctttatcta
     2341 aaaaatatca ggttaatgga cagaatagat attatgatgt cttaagaaaa aactcagaaa
     2401 gctttcaaga aaatatcgaa atgcaaatgg agattaaagc atataattta tcgaaggata
     2461 ttaaagatga cttatataaa aaaatggaag atagtgagag agtacactta aaggcggaag
     2521 taactacaat tttaactttg tctatatctt caatatttag ctttatatct cttgctgttg
     2581 tgatatttgt cggcgtaaat ctaattatta ataaagagat aaattctctc taccttatag
     2641 gatatttact agctgctatg aagataaaag actctttaga tgcatctaaa gagggcttga
     2701 tggaaatatt ttatttatcg cccaaaatag aaagattaaa agaaattcaa aatcaagatt
     2761 tacaagaagg cgatgactat agcttaaaaa aatttgatat tgatctaaaa gatgttgagt
     2821 ttgcctacaa taaagacgca aaagttttaa atggtgtaag ttttaaagct aagcagggag
     2881 aggtcactgc tttggtaggt gcaagtggct gcggtaaaac aactatcttg aaacttatat
     2941 caagacttta tgattatgac aagggacaaa tcttaatcga tggcaaagat ataaaagaaa
     3001 tatcaacaga atctcttttt gataaggtct ctatagtttt ccaagatgtg gttctcttta
     3061 atcaaagcgt tatggaaaat attagaatcg gaaagcaaga tgcaagtgac gaagaggtta
     3121 aaagagcagc aaaacttgca aattgcacag attttataga aaaaatggat aaaggtttcg
     3181 atacagttat tggtgagaac ggagctgagc tatcaggagg agaaagacaa agattatcaa
     3241 tagccagagc cttcttaaaa gatgcgccga tattgatctt agatgagata acagcaagcc
     3301 ttgatgttaa caacgagaaa aagattcaag agtctttaaa taatttagtt aaagataaaa
     3361 ctgttgtaat catttcacat agaatgaaat ccatagaaaa tgcagacaag atagtagttc
     3421 ttcaaaacgg aagagtagaa agcgaaggta agcatgaaga gcttttacaa aaatcaaaaa
     3481 tttacaaaaa tttaatagaa aagacaaaaa tggcagaaga atttatttat taggaggact
     3541 acaatggata ataaaaaatt aaaagtaaaa gatttagtaa gcatcggtgt ttttggcgta
     3601 atttattttg ccttcatgtt tggagttggt atgatgggct tgattccaat attgttctta
     3661 atatacccga cagtattagc catagttgca ggaactgttg ttatgttatt tatggctaag
     3721 gttcaaaagc catgggcact atttatattt ggtatgatat caccacttgt gatgtttgca
     3781 gctggtcata cctacgtagt tgtggtttta tcacttatag taatgataat agcagaatta
     3841 attagaaaga ttggtaatta taattcattt aaatacaata tgctttctta tgcaatcttc
     3901 agcacatgga tatgtagctc tttaatgcaa atgcttttag caaaagaaaa atatatggag
     3961 tggtctttga tgacgatggg aaaagattat gttgatgtgt tagaaaagtt aataacttat
     4021 cctcatatgg ctttagtagc cttaggtgct ttcttaggag gaattcttgg agcatatata
     4081 ggcaaggctc tattgaaaaa acactttgaa aaagcaggca ttgtataatg cctacttttt
     4141 cttcaaatgc tccctttaat cccaatccga tctctaagat attagtcgta tttatcacag
     4201 gcttaactgt tatgcatagc ataaacatcc gttttgagct ggcaattgtt tgtcttattg
     4261 gtattttgct ttatttgaat ggatacaaaa aaacactttt caaatggatt atattgtgtg
     4321 ggatacttta ctccttgcct aattttatgg tgttatctga attaaaccct atagttaaga
     4381 tgtttttgag tatacctatt gttattagaa tgtttatttt accatttata gcagcaagct
     4441 ttatgataaa gacctcggat gtaggcgcaa taatttcatc gatggataag cttaagattt
     4501 caaagaatgt atccatacct attgcggtta tgtttagatt cttcccatct tttaaggagg
     4561 agaagaaaaa catcaaaatg gctatgagag taagagggat aagttttaaa aacccagtca
     4621 aatatcttga atatgtttct atgccactac tcattatatc atctaatata tcagatgaca
     4681 ttgcaaaagc ggcagaaaca aaggcaatag aaaatccaat tgccaagacc agatacattc
     4741 gcgtaaagat acagctaatt gattttgttt atgttttagc ggttgctgga cttattgtgg
     4801 gaggcttaat atggttgaaa taaaaaattt aagtcttgat tatggtgaag agcgtatatt
     4861 agatgatata tcactatcca tagccgaggg agagtgcgtg ctatttacag gaaaaagtgg
     4921 aaatggtaag tcatctttaa taaattcaat caatggacta gctgtaaggt atgataacgc
     4981 aaagacaaag ggcgaaataa ttattgatgg taagaatata aaaaatttgg aactttatca
     5041 aatctcaatg cttgtttcaa ctgtttttca aaatcctaag acctattttt ttaatgtcaa
     5101 tacgacatta gaattattat tttatttgga aaatatcggt cttgcaagag aagagatgga
     5161 caggcgtttg aaggatatgc ttaagatatt cccgataaaa aatcttttga acagaaatat
     5221 atttaatcta tccggcggtg aaaaacaaat tctttgcatt gcagcttctt atatagcagg
     5281 tacaaagatt atagttatgg atgagccttc atcgaattta gatattaaaa gcataagtgt
     5341 tttggcaaag atgctaaaga tattaaaaga gaaaggtata agcataattg ttgcagagca
     5401 tagaatttat tatttgatgg acatagttga ccgtgtattt ttaatagata aagg
//
LOCUS       DAVKHZ010000095         5365 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000095 DAVKHZ010000000
VERSION     DAVKHZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.095"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(45..2603)
                     /locus_tag="VWW99_001902"
     CDS             complement(45..2603)
                     /locus_tag="VWW99_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9539699.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            3151..4351
                     /locus_tag="VWW99_001903"
                     /pseudo
     CDS             3151..4351
                     /locus_tag="VWW99_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4466..5269)
                     /locus_tag="VWW99_001904"
     CDS             complement(4466..5269)
                     /locus_tag="VWW99_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9539700.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT         1742 a         1163 c          843 g         1617 t
ORIGIN      
        1 ctaaagaaat gaaaatcata catagaaatt tttaaaaatt ttctctatcg taaacctgcc
       61 actagttcag aaactatact agctgattcc aaacgtaata tggtcatctc atctaccttc
      121 cctaataatg gcattgcacc ataccaaaca atagtcttat tcaccaaaag aataggcgtt
      181 actatttttg attgagtaaa agacacttta attttctggc tcttcaactg gtctagccat
      241 gctttatttg caaccgtctc tggtatacat atctcaattt gactaatcct aaattctttt
      301 agaaaattca tcagttttgt ctggtgcaca taaggtaaaa taagcagaca ttcctgtctt
      361 tcccctgcta agtcctctct caacaccttc tcatatctac tatcaacata aacgaattgt
      421 ttctcctcac cctcgatgac acgataatcc atctttcgat aagctacttg tcgtttctga
      481 aacatctttt ctaaataagg aacatgaata tccacataat cgaaaatacg caccaaagac
      541 ttatccttgt agtttctatg aatccgacct gcatactgaa ttaaattatt tttccaagaa
      601 aagggtgctg ccaagataag cgtgtccaac tgaggtaagt caaaaccttc gccaatgtat
      661 tttccagtag acaacaaaac aaacccttta tctaactgtt ctaacgtctc cagtaaactc
      721 gttctttctc ggactttggt ttttccgcta ataatgtaac agccatcaac ctctttctct
      781 ttcaataact tttcaaagac atctatctgt tgaattcgat taactaaaac caagatattc
      841 cgtccttctg ccacttgggc tagaatatcc ttgagaatca actgattcct cgctgagtct
      901 gtagcaatcc aatcactgag ctgtataaaa ttacttgctt tggtcttttc aatctccaaa
      961 tgaccaaaag aagtgaatct taattgcaat tgccgtttaa aatccgtttc cctcttatca
     1021 gcagtatgga gtatctcacc aattctctga aaaacaatag gctcatgacc attcttacgc
     1081 tcaggcgtag ccgtcaaacc gtaaagatac ttccctctaa actgagcaac aactttttca
     1141 aacatcaagg cagagacatg atgacactca tccacaatca tcatctcata ctcatccaaa
     1201 agactttgac tattttccaa cttaaataga gattgaatca taacgacatc aaccagttta
     1261 ctcagccatt tcttagtccc accgtactgt ccaacatagc cgattacctt ttcacgacct
     1321 gatgccgtat aacggatagc ctcctcctct tcgaaagtca aaaagcagtt taagcgatct
     1381 agccattggt ctaagagttg cttattatgg actagaataa ttgtttttgt tttccgttca
     1441 gagataagag cagcacctaa aacggtcttc ccaaaaccag tctccgcatg aagtaaacca
     1501 ttttctttag aagtcatatc tgacagggct aactcttgct caaaagtgag ttctccctta
     1561 aattccacgc taatagacct ttgtaccttt ctcctatctt ccacagatac ctgcttaaat
     1621 ttatcttgca atggatacag caaacctctt ggcaaccata aataatgatc ggattctcca
     1681 aataaataca ttctctcagg aatttgatag gttggctgtc gcatagcctg ctttaaataa
     1741 aattcgggat tagaaaatga agccatattt ttcaagaaaa acagtgtctt gggtgtcaca
     1801 gacgattttt caagttggat catattggat aagacgatct tcaactcctt gtccaattct
     1861 tgcttgccta actcctcttg gattaacagt gccactttag cagttgaaat cttctgaatt
     1921 tcttgtagat acctccattg atcttcataa ggctgaaact gttcatccac aaagaccgtt
     1981 cgcccttgat gataagcttc tccttgaaaa ggaagggaaa ttaaattatc aaatccacct
     2041 ttaggaagaa catcctgatt tggaaacatg cgatcaaaag aatcaaagga cagttgcata
     2101 ctttcctgca tagccaattc taatagtttc tttccaaaca ggcgagcctt tcgactcaga
     2161 atcgcttcct caaagaagaa ccaaatatgg agtccgtaac ccgaacgaga aatctctaaa
     2221 tgggcttcca tctggcgttc cctggctatt cttcgaatgg ttaaaccagc ttctttccaa
     2281 tctccttcat ccaaatccag taccaaaaaa taacagctat catctaagtg cataggaaag
     2341 ataccgatag ctgcctcccc acgaaaatga gatttcaaaa cggagttcgt caatgtctgg
     2401 aaactccgtt tttcaggtgg caactgcttc caaccataat cataggaagg aaaatactga
     2461 attttccctt gttcattgat aaaattctta gcataaacat cataacggcc agcaaacaca
     2521 gaggtaaaca attgtaattt ttcttgcgtc gtcaagcgac tactttgaag ttggaacatc
     2581 tcctcgaact gagagcgttc cataacaaac tgactcttgt ctttaagacg tttatagtgg
     2641 aatgaaataa gatatgaaca aattgattag gaaaatcaaa ttaatttata gaaatctttt
     2701 agcagtcatg gcgtcctatt ccagattcaa aatgctatac acaagaaaaa ctcactatcc
     2761 ccattaaaag aagaaaagac atcaaggact tctaccacaa ttccatccaa agacacaaaa
     2821 acagccataa gagtcacctc cttgattcct ataggctgat tataacaaga ctggctgaaa
     2881 ttgtacatga aaataaaatc ctaatagtac tcattttgta tgtgactaat attccgtctc
     2941 gctccagaag gtacgaagta aatagagttt aaacgagcag tctataaaat tcaaaaaacg
     3001 catattatca agcgacctaa aactttgggc tctataatat ttgtagtggg taaattccct
     3061 atggatatta tggagcctat tttgggtaga aaaaaagtcc cataagatct ataatgaaaa
     3121 gcgacaaaac aactcattag aaagaatcat atggaacaat tacattttat cacaaaatta
     3181 ctagacatta aagaccctat aaaattcctt accttgaaac gactggtatg cctactagaa
     3241 ttctccttag aaaacgtcgt tttaagtgct atcattgctt gaaaatgatg gtcgctgaaa
     3301 cttctatcgt caagaaaaat caccaaattc ctcgtattat caaccaaaag attgcgcaaa
     3361 agttaattga aaagatttct atgaccgata ttgcccatca gctggccatt tcaacttcaa
     3421 ctgtcattcg aaagctcaat gacttccact ttgagtgtaa ttttagaaat ctgcctaaga
     3481 ttatgtcttg ggacgttgaa acagtccggg gagtgactgt ttcaatcggg agatggagat
     3541 gagctttatt gcgcaagatt ttgaaaagct cgatatcatc actgttcttg agggtagaac
     3601 acaagctgtc atccgagatc actttcttaa atatgataga gccgtccgat gtcgcgtcaa
     3661 aattattact atggatatgt ttagtcctta ctatgactta gctaaacagc ttcgctttca
     3721 aatttctagg ctcaggctga aacagtctcc cagactgttt cactcccgaa tgctaaaatc
     3781 gttcttgatc gctttcatat tgtacaacat cttagccgtg ctatgagtcg tgtgcgtgtc
     3841 caaatcatga atcagtttca tcgaaaatcc catgaataca aggctatcaa gcgctactgg
     3901 aaactcattc aacaggatag ccgtaaactc agcgataaac atttttatcg ccctactttt
     3961 cgtatgcatt taaccaataa agagatttta gacaagcttt tgagctattc acaagacttg
     4021 aaacatcact atcagctcta tcaactcttg ctgtttcact ttcagaataa ggaaccggag
     4081 aaatttttcg aacttatcga ggacaatctt aagcaggttc atcctatttt tcagactgtc
     4141 tttaaaacct tcctcaaaga taaagaaaag gttatcaacg cccttcaact acactattct
     4201 aatgccaaac tggaagcgac caataatctc atcaaactta tcaagcgcaa tgcctttggt
     4261 tttcgaaact ttgaaaactt caaaaaaacg gatttttatc gctctgaaca tcaaaaaaga
     4321 aaggacgaaa tttgtccttt ctcgaactta gcttttcttc aacccactac aattgacaaa
     4381 gagccaaact ttgatgatat acgttttctt gtgagaatat ttacttcatt ttttcctaaa
     4441 attcaatgtt tactcagtat ttggattatg aaaaatcgag gtctaaatct agatacattt
     4501 tttctgaaga caaatcattt tgaccaccga gcaagagatt ttcaaaaaaa gctgttaaaa
     4561 actcaggacg tcgctgtaaa atctttgcat tatctaatac caaggcatca cgaaaatact
     4621 tggaatgttg ctgaaatggt atattatcaa tatcaaaacc aaactcacga agatactgaa
     4681 tcaaaaagac cgttactgtc cgagtgtttc cttcgcgaaa tggatgaatc tgccagattc
     4741 ctgaaataaa atgctggatt tgtttaacca catccgcctg agttagtgtc gcatatgcaa
     4801 cttgtttttc ctgattaaaa tcataatcta aggtcatttg aatcatggag taatcagagt
     4861 acacaacact ttcaccattc aaaacaggtt cattctttgt gatattggtc tgacgaaatt
     4921 gccccaccgg aatagagggt tcaaatatat cttgaaacaa ctccttatga atagcaagta
     4981 aggtcgcagg actaaagcta aagcctcttc gagacaatag ttctacaata cgtagagaaa
     5041 ccaagtctgc ctcttccgta cttgcatcaa tagtatggtg ataagccgtt gcatcctcat
     5101 aaacctgctc ataagtcagt tctccccggg actgtttctc agccaaagat tccatatacg
     5161 ctgatggcac tagattgtca actttctgca gaccaaaacc tatccgccat aaatcacgct
     5221 tcgcttcata agacaagttt ggattgtcaa tgttgtaagt tggttgcata gaaatatcct
     5281 ttcaattgtt tttcttcatt accagtaaat cacgaaattg gttggaaact ggcttaaact
     5341 ataaaaagtc ctcacaatag gttct
//
LOCUS       DAVKHZ010000096         5346 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000096 DAVKHZ010000000
VERSION     DAVKHZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.096"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            47..994
                     /locus_tag="VWW99_001905"
     CDS             47..994
                     /locus_tag="VWW99_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU9539701.1"
                     /translation="MTEEKFQKIIQSKDTETLAFILESTPYHLSIDILEDPSQTEISL
                     MTKLVNDYRWAYAESPSDIIVTLFALRYVYHNIKVLLKSKAAIKKDFSKLLIPIGIFD
                     IESLKHLVSSLHSDTLPDFMVREVESIWNEYETFNNIRVLDVGADLAYFKHLKLLSNE
                     LDEVLSQVIVEMIDFYNIITVKRGLSQNKSHGDILQLLSDEGSISAKEFIYIVENQEI
                     FVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLA
                     RYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
     gene            984..1304
                     /locus_tag="VWW99_001906"
     CDS             984..1304
                     /locus_tag="VWW99_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU9539702.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            1367..3142
                     /locus_tag="VWW99_001907"
     CDS             1367..3142
                     /locus_tag="VWW99_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU9539703.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTISIGQKVSTGDILGTVKETEVVNHKIMVPYGISGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            3143..4528
                     /locus_tag="VWW99_001908"
     CDS             3143..4528
                     /locus_tag="VWW99_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU9539704.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            4551..5162
                     /locus_tag="VWW99_001909"
     CDS             4551..5162
                     /locus_tag="VWW99_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU9539705.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1773 a          763 c         1093 g         1717 t
ORIGIN      
        1 tttttcaaaa ataaatacga cgatttcggt aaaagaaaac gattttatta cagaagaaaa
       61 atttcaaaaa attatacaat ccaaagatac ggagacattg gcatttatct tagaatcaac
      121 tccctatcat ttatcgattg acatcttaga agatcctagt cagacagaga tttcgctaat
      181 gacaaaatta gtcaatgatt atagatgggc ctatgctgaa agtccgtctg atataattgt
      241 gactttattt gctttacgat atgtttatca taatatcaaa gttttattaa aatctaaggc
      301 ggcaattaag aaagattttt ctaaattatt aattccaata gggatttttg atatagaaag
      361 tttaaaacat ttagtttctt ccttacattc agatacactt cctgatttta tggttcgtga
      421 agtagaatca atttggaatg agtatgaaac ttttaataat attcgtgtac ttgatgtcgg
      481 agctgatcta gcatatttta aacatctgaa acttttatct aatgagttag atgaggtact
      541 gtctcaggtt attgtcgaaa tgattgactt ttataatatt attactgtaa aacgtggttt
      601 atctcaaaat aagagtcatg gggatatttt acaattactt tcagatgaag gaagtatttc
      661 tgctaaagaa tttatataca ttgtagaaaa tcaagaaata tttgtgtggt tcaataaaat
      721 aaatccaagc ttagattcaa tcttttcaac ttatgaattg aagatgcagg acgcaacaat
      781 ttcatcttct gagttagaat ttttatgtga tttactattg tataaaactt tagatcaagg
      841 aaggtacaat gtagaggggc cgttagttct tgctagatat ttattgggat gtgagtttga
      901 agtaaagaat ctcagaatga tcatatcagc tcttcaaaat acaattccct ttgaatcaat
      961 aaaagaaagg atacgcccac attatggaag ctaataagta taaaattggc ataattggta
     1021 gccgtgatat tattttacca tttagcatga ttgggtttga tatatttcct gcctaccaag
     1081 aacaagaagc tataaataca ctaagaaaat tagctcaatc tgattatggt gtcatttata
     1141 tcactgaaga cattgcttca atgatattag atacaattcg ccattatgat tcccaagttg
     1201 tgcctgctat tattttatta ccgactcata aacaaggttt aaatttagga ttaaaacgta
     1261 tagaggataa tgtagagaaa gcagtaggac acaatatttt ataataatgt acaaaattgt
     1321 ctgtaatatt attctataat ttttggactt agtaaggaga ataactttga ctcaagggaa
     1381 gattataaaa gtatcgggac ctctagttat tgcatcaggt atgcaggagg ctaatattca
     1441 agatatttgc cgtgtaggta agctagggtt aatcggtgaa attattgaaa tgagaagaga
     1501 tcaggcatct atccaagtct atgaagaaac atctggtctt ggtccgggag aacctgttgt
     1561 tacaactgga gaacctctct cggttgaatt agggcctgga ttgatttctc aaatgtttga
     1621 tggcatacaa cgcccattag atcgatttaa attggctact cataatgatt ttctagttcg
     1681 tggggtagaa gttccaagtt tggatagaga tattaagtgg cattttgatt ccactatatc
     1741 aattggtcaa aaagtgagta cgggtgatat tcttggaact gtcaaggaaa ccgaagtagt
     1801 taatcataaa attatggttc cttatggaat atctggcgaa gtcgtttcta ttgcctctgg
     1861 cgattttaca attgatgaag ttgtatatga aataaaaaaa ttggacggta gtttctataa
     1921 aggaacactt atgcaaaaat ggcctgtccg caaggcgcgt cctgtttcta aacgtttaat
     1981 cccagaagaa ccattaatca caggtcaacg agttattgat acattctttc cagtaaccaa
     2041 agggggagca gcagctgttc ctggaccgtt tggagcagga aagacagttg tacaacacca
     2101 agtagctaaa tttgccaatg ttgatattgt tatttatgtc ggttgtggag aacgtggaaa
     2161 tgaaatgacg gatgtactga atgagtttcc tgagttgatt gaccctaata ccggacaatc
     2221 aattatgcaa cggacagttc tgattgctaa tacttcaaat atgcctgttg ctgctcgtga
     2281 ggcttcaatt tatacaggaa ttaccatggc tgagtatttt cgtgatatgg gctactctgt
     2341 cgccattatg gctgattcaa cttcacgttg ggcagaagcg ctacgtgaaa tgtcaggacg
     2401 tctagaagaa atgcctggtg atgagggtta tcctgcttat ctgggaagtc gtatcgctga
     2461 atattatgaa agagcaggac gttctcaggt tctagggctt ccagaacgtg aaggaacgat
     2521 tactgctatt ggagctgtat cgccacctgg tggagatatt tcagaaccag ttactcaaaa
     2581 cactttacgg attgtgaaag ttttttgggg gcttgatgct ccgttggcac agcgacgtca
     2641 ttttcctgca attaactggc ttacatctta ttcactatat aaagacagtg tgggcactta
     2701 tatagatggt aaagagaaga cagattggaa tagtaaaata actcgtgcga tgaactactt
     2761 acaacgggaa tctagtttag aggaaattgt tcgtcttgtt ggaattgatt ctctgtctga
     2821 taatgaacga ctaacgatgg aaattgctaa acaaattcga gaagattatt tgcaacagaa
     2881 cgcttttgat tcggtagata cattcacttc gtttgcaaaa caagaagcaa tgctaagtaa
     2941 tattctcact tttgctgatc aggcaaatca tgctttagag ttgggttctt actttacaga
     3001 gattatggaa ggtaccgtgg cagttcgaga ccgtatggcg agaagtaaat atgtttcaga
     3061 agatagatta gatgaaatca aaattatatc aaatgagatt acacatcaaa ttcatttgat
     3121 attagaaaca ggaggtctat aaatgagtgt tataaaagaa tacagaactg ctagtgaagt
     3181 tgttgggcct cttatgattg ttgaacaagt aaataatgtg tcttacaatg agttagttga
     3241 aattcaactt cataatggag aaattcgtcg tggacaagtt ttagagatcc acgaagataa
     3301 agcaatggtt cagctttttg aaggatctag tggaataaat ttagaaaagt ctaaaattcg
     3361 ttttgctggt catgcattag aattggctgt atctgaggat atggttggtc gtatttttaa
     3421 tgggatggga aaaccaattg atggtggacc agatttaatt ccagagaaat atttagatat
     3481 tgatggtcaa gctattaatc ctgtatctag agattatcca gatgaattta ttcagacagg
     3541 gatctcctct attgatcatt tgaatactct tgtacgtggt caaaaattac cagtattttc
     3601 aggttcgggc ttacctcata atgaattagc tgctcagata gcaagacaag cgactgtttt
     3661 aaattctgat gaaaattttg cggttgtatt tgcagcaatg ggtattactt ttgaagaagc
     3721 tgagtttttt atggaagaac tcagaaaaac aggagcgatc gatcgttcgg ttttatttat
     3781 gaacttggca aatgatcctg caattgagcg tattgcaact ccccgcattg ctttaactgc
     3841 ggcagagtat ctagcttttg aaaaagatat gcacgttcta gttatcatga cggatatgac
     3901 taactattgt gaagcgttac gtgaagtctc ggcagctcgc cgtgaagttc cagggagacg
     3961 aggctatccg ggatatttat atacaaattt atcaactcta tacgaaaggg ctggtcgctt
     4021 agttggtaaa aaaggttcgg tgacacagat tcctatttta acaatgccag aagatgacat
     4081 aacacatcca attcctgatt taactggata cattactgaa gggcaaatta ttttgtcgca
     4141 tgagttgtat aatcaaggtt atcgtccacc aatcaatgtt ttaccttctc tctctcgatt
     4201 aaaagataag ggatctggag aaggtaaaac tcgtggagat catgctccaa ctatgaatca
     4261 actgtttgca gcctatgccc aagggaaaaa ggttgaagag ttagcagtag tattaggaga
     4321 atcggcttta tctgatgtag ataaattgta tgtgaggttt acaaagcgtt ttgaagaaga
     4381 gtacataaac caaggatttt ataaaaatcg aaatatagaa gatacgttga atcttgggtg
     4441 ggaattacta tcaattcttc ctagaacaga gttaaaacgt atcaaagatg atttgcttga
     4501 taaatactta cctttggtag aagtttaatc cggaaatgga gtgattatct atggtacgtt
     4561 tgaatgtaaa accaactcgt atggaattga ataacttaaa ggaacgtttg acaacagctg
     4621 aacgtggaca taagttatta aaggataaaa gagatgaatt gatgaggcga tttatttctt
     4681 tgattcgtga gaataatcaa cttcggaaag aagtggaaag ttatctaatt gataatctaa
     4741 aatcctttgc agttgctaaa tcattaaaga attctcaaat ggtggaggaa ttattttcaa
     4801 ttccatcgaa agaaattgaa ttatttgttg agaaagaaaa tatcatgagt gtaacagttc
     4861 ctagaatgca tatgaatatt acttctcaaa atgagaacag tgaatacagc tatttatctt
     4921 ctaatagtga aatggatgat gtatttgcta caatgaatag tttaatttat aaattactaa
     4981 gactggcaga agttgaaaaa acgtgtcagt taatggctga tgaaatagaa aaaacacgta
     5041 gacgtgtaaa tggtttagaa tactcgatta ttccaaactt gtcggaaact attcattata
     5101 tagaattgaa actagaggag gcagaaagag ccaatttagt tcgtattatg aaagtgaagt
     5161 agatccttta tttagattat taattagatg aacaaatatc agcttggata aggctttaag
     5221 cctttctaag ctttttttat tgacagtatc aggatatctt tttcaaaatt ttggtttgtt
     5281 agataatgaa aatgtttcta ctaatctaga tttaggatta gtaaatcgta aatgtaatta
     5341 tataga
//
LOCUS       DAVKHZ010000097         5256 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000097 DAVKHZ010000000
VERSION     DAVKHZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.097"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(40..906)
                     /locus_tag="VWW99_001910"
     CDS             complement(40..906)
                     /locus_tag="VWW99_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539706.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
     gene            complement(1020..2843)
                     /locus_tag="VWW99_001911"
     CDS             complement(1020..2843)
                     /locus_tag="VWW99_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9539707.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            complement(2830..3195)
                     /locus_tag="VWW99_001912"
     CDS             complement(2830..3195)
                     /locus_tag="VWW99_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9539708.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            complement(3200..3556)
                     /locus_tag="VWW99_001913"
     CDS             complement(3200..3556)
                     /locus_tag="VWW99_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539709.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            complement(4388..4840)
                     /locus_tag="VWW99_001914"
     CDS             complement(4388..4840)
                     /locus_tag="VWW99_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539710.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNTTIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            complement(4768..5256)
                     /locus_tag="VWW99_001915"
                     /pseudo
     CDS             complement(4768..5256)
                     /locus_tag="VWW99_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1527 a         1066 c          788 g         1875 t
ORIGIN      
        1 tagatttaaa aaacgatgct cactagaaca ccgtattcct tattctgtta tatctgcaaa
       61 ataatgttcg actttcaaag ccattgacat gttttcctca agtttgtaga gaaaatgagc
      121 agtctcaaaa tattctttta ctgaactttt agaagaaaaa ttttcactta cacttcccat
      181 caataaatac aaatcagcta atagataagt tgtcctgtac tccttacatt gctttatcgt
      241 agctgtgatt ttcgtaatag cttcttcaat atttttctgt aaccataggt atcgacaaac
      301 gttataattg aacttaattg atagttctaa ctcttcaatt gtgtttaaat taagctgatt
      361 cacttgatgc tctagcttgt ctctaatctc attaaaacgt tctaaatctt caatatcata
      421 ataaaaattg aatagagtgt ttgaaacttg aaggtagttc aaatcagata catttaactg
      481 aggtagcata ttctctaatc gtgtaattgc ctcttctttc tgtccataaa aataaaaatc
      541 tataagagac tttatccatt ccatgtaaaa tttatctgct agagataagc gatgggcttt
      601 tacattttcc aattcatata tatattttaa agattcataa ttacgatttg tgataaatgt
      661 ctgtgctaac ttcttaaact ctgataactc attgacttcc tctacaacct gctcatcaaa
      721 aaagtagtcc atactaactt ttaatttctt agcaagggca tgtaaaaaat ccgctccagg
      781 ggtgaactct ccactctcta atcgactaat ttgcccctgt ttacagatac cctcagctaa
      841 ttctcgctga gacattttta attcttttcg tctatttttt aatcttgttg ctaatagtgt
      901 acccacgttt gcatcttcct tttctatttg atttcaatgt taattgtaac atgattaaat
      961 ctaaatttca aatatatttt acaaaagata cacattgtat ataattattg gctagattcc
     1021 tatttttgaa aatctatccc atctcctttt gaaagtcctg tatttaagcg atttataaat
     1081 ttatctagcc tgactaccat cttttcatac ctgccccttt ccaattccag ttgattcccc
     1141 aggctcttca attttttctc aacaatatta gcactgatat tctcagttat attcagtttt
     1201 gaaagattta atttcatttc ttgcttactt tcaactgaag ccagtagata ttctgctact
     1261 tgattcaagg ttgacattcg ttcttgcaac tcattgattc tcaattcaga cgctgctagt
     1321 tcatgcacta actcatcttt aatcgtgaca taagattgat tttctacttc cagttcaatc
     1381 aaagcatcca cttccgcaat cttctcttta atcatatcga ctgtcgcttt tctgcgtaat
     1441 ggaatggttt gattttcata gctgaactgt ctaattaagg ttcgaccttt catataacaa
     1501 tttttctcgg cagcgtcttt gtggtagaca tagtaggagc tggtttccct gatgaaaacc
     1561 ttcaccttat cctcttccat atcaagctgc atgttcggca caaagataag tccctcctga
     1621 cgaataccaa acttgacctt gacgtaaatt ccatcatcca ctactttttc aatttgattg
     1681 agtgacaact ccacctcaaa ttcatgaacg gcatctctgt tactcttgaa ctcttgatag
     1741 gactcccaga acttctcatc gcttggaagt tctttttctt tggataactt ttcttcttga
     1801 taaagttgaa cgaaatcctc agtttctgga gcttgccaat cttttctatt tttaaaataa
     1861 tcttggaaaa actctacatc ataaagattt ttctggtcta gctctgtctc ctttacctcc
     1921 actcctgcaa attgaaaaga aacatgcttt tgtttaggat tgatagttag tccaaaaagt
     1981 tttgctttct gaagtaaatc atccatattc tcaactttca gcaataaaaa ttccatgaga
     2041 ctttctattt ctcttttggc aaagtagttc ttaaaaaatt cttctgtgta aggctgcttg
     2101 cgattgagtt gcttgccacg caccacctgt ttcatagttg agtccgtaat aaaaaaggtg
     2161 gcatgcttgt gacggaaatc catctccaca tgtagtagcg gagcattctt tttgaaatcc
     2221 tcaaagtccc tagaatgttc catcaaaaaa tagagtcgct gcttgagttc atacttgtga
     2281 ttagtcttac gatagacttc ataccgctgg tgagaatagc ggttctcaat gattttagca
     2341 cctgcgattt tagaaaaacg gtcagaaatc atgcgaagat ttcgctccac cttgtagtcc
     2401 cacttgagct ttttgtcaga attgctatct actgaattga taatgatgtg attgtgcagg
     2461 tggtctttat caacatgggt cgcaacgata aaacgaaatt tgccaccagt taattccttc
     2521 acagtctcat aacctatccg attgatttgt tcaggagtga gatgatcctc tggtgagaaa
     2581 gactgaatga tgtggtgagc gtgtattttt cgttgatttt cttccatcct gtcgtggcga
     2641 aaatcgtaaa gcgtatcgtt gctgatgaaa ttttcatgat acatctgcac catttcctca
     2701 tagctaggaa agtccagaaa attcttcatg ccatagtccg acaccaaggc aagattattg
     2761 gttttctcag gattgagaat gtacttgata agctttctgc ggtaactctt tccgtgaatg
     2821 gcaaagtgtt tagtgatgac catggaactc ctttagcttc tgcgcttgca gattaaattc
     2881 cttatcaact tcttttatca attcaccaat tccttttttc aactcctgca attcttcacc
     2941 agaaattaac tgagattgat taacacttcg agcaatctgg ttgatattat tgccaatcct
     3001 ctttaactca aacactaaat cttggtaacc gtttgtgtca atcgtgataa aattcatacc
     3061 aggatcgagt agagttcgtc tcgcatattc agaaaaggat ttacaaccac tttgatcgat
     3121 atttatattt agttggttca actcactatc agacaaaaac actttcttga gattggttcg
     3181 gtaacgatgt tccatgtgct tacctcatgt acttctcacg aaattcttta ctgaggggta
     3241 tggtgtttgc aacctcttga atcaattcct gcacgcacgt taagagaata gatacatgct
     3301 cttgggtgac ttgacgttct gactgtgcca agattaaaac ttcatgaacg tcacgactga
     3361 tttgttctag tttttgggat tgccagaggg caaaccatgt ctccatctgt ttttgtaaat
     3421 cagaggaaag taaactttta cgaagaaaat cagagaaatt agtctctccc cgctctctca
     3481 tcaaagctag aatttgcttt tcttctgctt ctgttaaacg aaattgtttc cgaatatcac
     3541 gcacatctcg tttcatactg ctacttcctt tccaaaatac ttgtactgac aatgtgagct
     3601 accatacaat gtcagtacac ttttccaaca actgattttc attcttgtaa ggctttgcga
     3661 gctcagatat tgtgtccaca atatcccaaa aatcatatcg ccagcctatc aaaacctacc
     3721 agttttgaca gctaacgata agataactta gtggctccgc ccccaaaccc ccaggtaaaa
     3781 ttcttagttg gatttttaaa agttgagaaa agtatagcag aagaaatttg gggaagttat
     3841 cacaaatcaa gtaaggacta atctctaaca ccactgcatg taccgtccta tatttatgat
     3901 cagctcccat cgtttacatt ttcaggtcac tgtcttttca taccaggaat tagaattgac
     3961 aaatttaatt agagaggtat tcatttaatt gttcacttct tcgctccatt tccattacag
     4021 aaatttcttc aatactatgt gttcagctga ctcccatgat tcgttgtact acgtcctaaa
     4081 cgctcacgga ataagccgta catctttcct agtttactct cgtaatttac gtatataggt
     4141 tacgacacct atttggattt tagagttcct catccgctat atatggcttc aagtcacgat
     4201 tctgtttgta gaaccttgat ttcgctaatt ccttcctatc ccccctacct cacgatattc
     4261 aggcttagga gtcgctattg gtttcatctg cagcaaatat ccacagagga cttggacctc
     4321 agatgtacac tatgcctgtc ggacaaagaa aaaagtaggc taaatttcta acctactttt
     4381 tctgactcta ctctgataaa gccgtctcat atatagacat tttatgcttg aaggaatctt
     4441 ttcctccctc taattgattg atgatatgat tttttatata tttttcacta acatatccct
     4501 gtgattcaat ccatccgtta actccatctg actccataat aacaacttga tctgtcatag
     4561 aatttgttac aattgtagta ttatgtgttg ctaaaataat ttgacgttga gctttcacat
     4621 ctctaaactg ctgaactaaa tgcctgtaaa tataacgatt gtctagattg tcttcaggct
     4681 gatcaataat caatggtctg aagtctttag aataatcact atatgctaat aaaaaatcaa
     4741 gcatggctac aactttttgt cccaaactta atacaccaac ttctttatag agaatatcaa
     4801 gttgttgcga agattctttc gagttaatat taaactccaa aaatcgtgta ttttctttag
     4861 aaaaaatttt tcgtttatat gcacctaata agtgagaacc ttttttcaaa atgtcataac
     4921 tattgaaatg agcaatatag ttaactattt tttgattatt gaaatccttc attatagtca
     4981 gtgaatgttg ataacttcca tctttctcac ttataatatt ttcacttaac cattgattaa
     5041 cccatgattc ttgttcataa tcatatatac atacaatatg caatttatct gctgcactaa
     5101 tttctactcc atgtaaaata tgaggatgta tatcataaat cggataattt ttcattatga
     5161 ttgagactgc catttgtaac tttttaatac ctttggtagt attatgatca gttactacaa
     5221 ctatttctat tccatttttt atgattgcct ctgcta
//
LOCUS       DAVKHZ010000098         5116 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000098 DAVKHZ010000000
VERSION     DAVKHZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.098"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            556..1902
                     /locus_tag="VWW99_001916"
     CDS             556..1902
                     /locus_tag="VWW99_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9539711.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
     gene            2084..3307
                     /gene="dcm"
                     /locus_tag="VWW99_001917"
     CDS             2084..3307
                     /gene="dcm"
                     /locus_tag="VWW99_001917"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEU9539712.1"
                     /translation="MIWVLDLFAGIGGFRMGMEAQGHECLGFCEIDKFARKSYKSIFQ
                     TEGEIEFHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIA
                     RAAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPKNR
                     ERVFIIGHSRKKGTRLLFPFRREGQATNSETLKALGNLNPSRSGMSGKVYYSEGLAPT
                     LVRGKGEGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRYGIVVVGDLPTSF
                     KETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINL
                     ERPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYQVAGVLIDGQFYRLRIR
                     RITPKECFRLHGFPD"
     gene            3335..3445
                     /locus_tag="VWW99_001918"
     CDS             3335..3445
                     /locus_tag="VWW99_001918"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9539713.1"
                     /translation="MNSQLYKQVGNSVTVPVIAAIAKKLKEIEEKDKSIK"
     gene            3429..3878
                     /locus_tag="VWW99_001919"
                     /pseudo
     CDS             3429..3878
                     /locus_tag="VWW99_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3871..4251
                     /locus_tag="VWW99_001920"
     CDS             3871..4251
                     /locus_tag="VWW99_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9539714.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIGLPFVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            4264..4497
                     /locus_tag="VWW99_001921"
     CDS             4264..4497
                     /locus_tag="VWW99_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9539715.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQILDLDYEDLFRR"
     gene            4500..5087
                     /locus_tag="VWW99_001922"
     CDS             4500..5087
                     /locus_tag="VWW99_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9539716.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQVLYGASLFS
                     FLWMYGQRKQVVKVNMKTRMKCLGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNFLVYSLLLF"
BASE COUNT         1605 a          866 c         1062 g         1583 t
ORIGIN      
        1 tggaatgtta gtgcttatat ggggggaaca gaatcctctc ttgattgaag acaagctagt
       61 cattaggctg gtttgtcttt ttgtcaactg tagtgggttg atataatagt attagtgagt
      121 gggataaaag tttcatttag tttattcagt acaaatttaa cgggtcaaga tttatatact
      181 agtggtgttt ttggggctga gagaagtatc ttgattttat gtgtggtttt tatacttaca
      241 gttgttctgc tccaaagagc ttgtagagaa gaattagctc ataaaggaga ttgattattt
      301 tgatatcaaa aaaatgcaca ggataacctg atgcattttt ttagcgacaa tgcttgctac
      361 ttccttctgt cgaatttaga caattttaaa ccccaattat tcaccccaaa tctaaaaacc
      421 atccagaatc cttgccttag cttagatcct ggatggtttc ttttttcacc caatgggtgt
      481 tttttactag acaaaaaaga gtttcccctt tatggtataa gtgtagaaaa aaacacaaaa
      541 agaaaggaaa ctcacatgaa cagtttacca aatcatcact tccaaaacaa gtctttttac
      601 caactatctt tcgatggagg tcatttaacc cagtatggtg gtcttatctt ttttcaggaa
      661 cttttttccc agctgaaact aaaagagcgg atttctaagt atttagtaac gaatgaccaa
      721 cgccgctact gtcgttattc ggattcagat atccttgtcc agttcctctt tcaactgtta
      781 acaggttatg gaacggacta tgcttgtaaa gaattgtcag ctgatgccta ctttccaaaa
      841 ttattggaag gagggcagct tgcttcacag ccaaccttat cccgttttct ttccagaact
      901 gacgaggaaa cagtccatag tttgcgatgc ctcaaccttg aattggtcga attcttttta
      961 cagtttcacc agctaaacca actcattgta gatatcgatt ctacccattt cacaacttat
     1021 ggcaagcaag aaggtgttgc ttataacgcc cactatcgtg ctcatggcta tcatcctctt
     1081 tatgctttcg aggggaagac aggttattgt ttcaatgccc agcttcgtcc tggtaatcgt
     1141 tattgttctg aagaggcaga cagctttatc acacctgttt tagaacggtt taatcaactt
     1201 ctctttcgaa tggatagtgg ctttgcgacc ccaaaattat acgatttaat tgaaaaaaca
     1261 gggcaatact acctcataaa actcaagaaa aatactgttc tgagccgtct tggagacctt
     1321 tccctccctt gcccacagga tgaggactta accatcttgc cccactccgc ctactcagaa
     1381 actctctatc aagcaggatc ttggtcgcac aagcgtcgtg tctgccagtt ctctgaacga
     1441 aaagaaggaa acttgttcta cgatgttatt tctctcgtta caaatatgac gagtggaaca
     1501 agccaagacc agtttcagct ttatcgtgga cgtggtcaag ccgagaattt catcaaggag
     1561 atgaaggagg gattttttgg cgataaaacg gatagttcaa ccttaatcaa aaacgaagtt
     1621 cgtatgatga tgagctgtat cgcctacaat ctctatcttt ttctcaaaca tctagctgga
     1681 ggtgacttcc aaactttaac aatcaaacgc ttccgccatc tttttcttca cgtggtagga
     1741 aaatgtgttc gaacaggacg caagcagctc ctcaaattgt ctagtctcta tgcctattcc
     1801 gaattgtttt cagcacttta ttctaggatt agaaaagtca acctgaatct tcctgttcct
     1861 tatgaaccac ctagaagaaa agcgtcgtta atgatgcatt aaagaacagt cgagatgaaa
     1921 aaatcgtgtg acgaaccaag ggaggagtct gcccttttga ggaaatctag cgaggaaaaa
     1981 cgatactgga acagcagaaa gtaaaactga cctcatgagg aggaagaaag tggctcatga
     2041 ggtcaggggt tttgtaagtt acatctagtt gagagaggta tgaatgattt gggttttaga
     2101 tttatttgct gggataggag gctttaggat gggaatggaa gctcaagggc atgagtgctt
     2161 gggattttgt gagattgata aatttgctag gaaatcttat aagtccattt ttcaaacgga
     2221 aggagaaata gaatttcatg acatacgaga tgtttcagat gacgaattta aaaaacttag
     2281 agggaaagtc gatgtcatct gtgggggatt cccttgtcaa gcattttcaa tcgcaggaag
     2341 acgattggga tttgaagata ctagaggaac tttgttcttt gaaattgctc gggcggccaa
     2401 acaaatccaa ccacgttttc tttttcttga aaatgttaaa ggcctactca atcacgataa
     2461 gggacggacg ttcaccacaa tacttaccac acttgatgag ttggggtttg atgttgagtg
     2521 gcaggtgctt aacagtaagg attttggcgt tcccaaaaac agagagaggg tgtttattat
     2581 cggacattct agaaagaaag gtaccagact cttatttcct ttcagacgag aaggtcaagc
     2641 aactaactct gagactttga aagcactagg aaatttgaat ccatcaagaa gtggaatgag
     2701 cggtaaagtc tattattcag aaggtcttgc gccaacctta gttcgtggta aaggagaagg
     2761 atttaaagtt gcgattcctt gtatgacacc agatagatta gacaagagac aaaatgggag
     2821 acgctttaag gaaaatcaag agccgatgtt tactttaaat actcaggatc gttatggcat
     2881 tgtcgttgtt ggagatttac cgactagctt taaggagaca ggtcgtgtct atggaagtga
     2941 gggcttatct ccaacactga ctacgatgca aggaggagat aaaatcccca aaatactaat
     3001 tccagaacca atccaatttc taaaagtacg ggaagcaacg aaaaaaggat atgctcaagc
     3061 agagattggg gattcaatca atttagaaag accaagttct cagtatcgtc gtggtagagt
     3121 tggaaaaggt atagctaata cgttaacaac tagtggtcaa atgggagtag tagtggctag
     3181 ctatgaagga gaagataaac aagtttacca agtagctggt gttttaatag atggacaatt
     3241 ttaccgtttg agaatacgac gaatcactcc taaagagtgt tttcgattgc atggctttcc
     3301 tgattaggct tttgaagctg ctagaaaagt ctctattaat agtcagctct ataaacaagt
     3361 tggtaatagt gtaaccgttc ctgtgattgc cgcaatcgca aagaaattaa aagaaataga
     3421 ggaaaaagat aaaagcatta aataaagaat caatactaga ttgtgatgaa ttagaaacag
     3481 aattacatga tgcagaaatc aaacagctgg atgaacaaat atttttgatg cccaattatc
     3541 catgtgagtt tgaggtaaca tttttagatg attaccataa aaaacataat tacccactat
     3601 tttatgaatc ctatcttcaa aacgttatgg aattccttga aagtcaggat ataaagaacg
     3661 gagttaatgc ctttgtagat gataatcaga atcttgtttt tgttttatat tgacaagact
     3721 atcgagccga gggaaaagag gggatactta caacccaagt aactgtaaaa gcttatgatg
     3781 aagacaagaa atcgattaac ttctcaaagt tgttagattc cttaatcgtc tcagaatatc
     3841 aaatggaagc aaatttgttg gaggtctccc atgattgatc tctatctaag taaaaatagc
     3901 caaagaaatc aacttctttt agacttcttc caaaactatg gcattgaggt atcttgtcat
     3961 tcagtttctg aaatgacaaa ggacaaatta attgagatga tgagctattc ttcagattgt
     4021 tttgagtttt tatctcccaa tttattacga tttaagaacc gtgacaatct aagattaacg
     4081 gatttcgttg agataatttt aaaaaatcct gaactaacca tcggacttcc ttttgtggtt
     4141 tcaaataagc gggtttatcc aagtctgaat ttggaagagg ctagagctct attgccaaga
     4201 gatacgaaac aattgattta catggcacaa acacattatt tatctaacta aaggagaagt
     4261 aatatggctg aacgattttg ggagaacttg tccattattt tggctgaaag aaatatcagc
     4321 tggattgagt taaccagaaa aatgtttgcg ggagagtttc actatccaag tgaattgaat
     4381 cgtttatatc aaaagattcg ccactacaag atggaacaac gaatgcctca aagtccatgg
     4441 gtagaaagga ttgtgcagat attagattta gattatgaag atttatttcg gaggtaacta
     4501 tgaaaagaat aattccagtt tatatattcc aacaagtaaa tgtcctattg gtatctctat
     4561 acttattgaa atttctttgt atcggtgagt taactatact acaggttctc tatggtgcgt
     4621 ctcttttttc ttttttatgg atgtatggtc aacgaaaaca agtggtcaag gtcaatatga
     4681 aaactaggat gaaatgcctt ggtattggac tagctagtct actgattata agtctatgtt
     4741 ttagcctgat tcatgctcaa ggaagcacga atcaaacaaa tttaattggc cttcaacatc
     4801 aagttccttg gttttcattt ttattgttct taatcaatgc gagtatggtt gaagaatttc
     4861 tgtatcgtga aattttatgg aacttggtta gaaaattaga tattcgagtt gctttgacaa
     4921 gtgttttatt tgccttagta catcatccag gaaccattct agcttggtgt ctgtacgttt
     4981 cacttggaat gtttttaggt atggtgcgct ataaatctga cttatgggga agtatggggt
     5041 tacatttggt gtggaacttt ttagtttata gtttgctgct tttttaaaat aggtgacctg
     5101 atttatctca taaatc
//
LOCUS       DAVKHZ010000099         4892 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000099 DAVKHZ010000000
VERSION     DAVKHZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.099"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            73..669
                     /locus_tag="VWW99_001923"
                     /pseudo
     CDS             73..669
                     /locus_tag="VWW99_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            745..1194
                     /locus_tag="VWW99_001924"
                     /pseudo
     CDS             745..1194
                     /locus_tag="VWW99_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1207..2298
                     /locus_tag="VWW99_001925"
     CDS             1207..2298
                     /locus_tag="VWW99_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539717.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            2288..3923
                     /locus_tag="VWW99_001926"
                     /pseudo
     CDS             2288..3923
                     /locus_tag="VWW99_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4026..4718)
                     /locus_tag="VWW99_001927"
     CDS             complement(4026..4718)
                     /locus_tag="VWW99_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9539718.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1530 a         1043 c          845 g         1474 t
ORIGIN      
        1 tagaattgta ggttttaaaa acgcttacat tgttttaaaa taaacttaaa taacacaacg
       61 gaggtatcca tcatgaaaaa gaaatggatg tattatgctg cttgttcttc taatgaatct
      121 gccgatgaca gttcatctga taaaggagac ggtggttcgc tagtcgttta ttgaccaaac
      181 tcagaaggct taattggagc aactattcct gcctttgaag aaaaatatgg tatcaaaata
      241 gaactgattc aagctggtac tggagaactt ttcaaaaaac tagagtcaga aaaagaagtt
      301 cctgtagctg atgttatctt tggtggttct tatacacaat atgctaccca cggagaactc
      361 tttgaaaact atatttcaaa agaaaatgat aatgttatca aagaatatca aaacacaact
      421 ggcttctcta ctccttatac actagatggt aatgttttaa tcgtccaccc tgatttaact
      481 aaaggcatga acatcgaagg atatagcgac cttctcaaac ctgaactaaa aggaaaaatc
      541 gcaactgctg acccagcaaa ctcttctagc gcctttgctc aattaacaaa tatgctacaa
      601 gctcaaggtg gttacaaaga tgataaggct tggtcttatg taaaagatct tttcacactt
      661 attgatggta tagtaaaatg aaataagaac aggacaaatt aatcaggaca gtcaaatcga
      721 tttctaacaa tgttttagaa gtagatgtgt actattctag tttcaatata ctataaaatc
      781 ggttcaagtt catctggtgt ctataaagca gtcgctgatg gagaaatgac tgttggtctc
      841 tcttatgaag atccagcagt taaactctta aatgacggag ctaacattaa ggtagtctat
      901 ccaaaagaag gaaccgtctt cctaccttct agtgctgcta tcgttaaaaa atctaaaaat
      961 atggaaaatg ccaagaaatt tatcgatttt attatctctc aagaagtaca agatacactt
     1021 ggtacaacca ctactaaccg tcctgttcgt aaaaatgcta aaacaagcga aaacatgaaa
     1081 ccaattgaca aaatcaaaac actcacttaa gattatgatt atgtcatcaa gaataaatca
     1141 gatatcgtta agaaatacaa cgaagtcttt acagatatcc aatctaaaca gtaaaagagg
     1201 ttcactatga gtgagatcga aattattaac gccaaaaaaa tctaccacga tgtccctgtt
     1261 attgagaatt tgaacattac aattccaaaa ggaagtctct ttacccttct tggagcttca
     1321 ggatgtggga aaacgaccct tcttcgtatg attgcaggtt tcaacagtat caaagatgga
     1381 gaattttact tcgatgatac aaaaatcaat aatatggaac ccagcaaacg caatatcggg
     1441 ctggttttcc aaaactacgc tattttccca catttgactg tccgagacaa cgttgctttt
     1501 ggtcttatgc aaaagaaggt tccaaaagaa gaattgattc aacagaccaa caagtatctt
     1561 gaactcatgc aaattgctca atatgcggat cgaaagcccg ataaactcag tggtggacaa
     1621 caacaacgtg tcaccttggc atgcgcctta gcggttaatc caagtgttct cctcatggac
     1681 gagccactta gtaatctgga ggccaaactt cgcttggata tgcgtcaaac catccgagaa
     1741 atccaacacg aagtgggaat tacaactgtt tatgtaaccc acgaccaaga agaagccatg
     1801 gctatttcag accaaattgc tgttatgaaa gatggggtga tccaacaaat cggccgacca
     1861 aaagaactct atcataaacc agctaatgag tttgtggcaa cctttatcgg acgcacaaat
     1921 attatccctg ccaatcttga aaaacggagc gacggcgctt atatcgtctt ttcagatggc
     1981 tatgcccttc gaatgctagc tcttgatcag gttgaggagc aagctattca tgtaagcatt
     2041 cgtcccgaag agtttatcaa agatgaatct ggagatatcg aaggaactat tagcgatagc
     2101 gtctatcttg gactaaatac ggagtatttc attgagacag gttttgcctc aaaaattcaa
     2161 gttagtgaag aatcaacttt tgaagaagat ctacaaaaag gcaatcgtat tcgtctacga
     2221 atcaatacgc aaaaattaaa catcttttct gcagatggtt cccaaaacct gataaaagga
     2281 gtcaaccatg gaatgtaaaa aactaaatat ttggacagcc tcctctttct tcctctttct
     2341 tacctatctt gtctttctcg tttatcctat cgttaccgtg ctcaagcaag cacttataca
     2401 tgaaggacaa ttctcactag ctaattttgt cactttcttt agtaaagcct actactctga
     2461 gacactagtc aacagtttca gggtttccat taccgctact gtcacttcct tagttgtagg
     2521 aaccctatta gcttatctct tctctatgta tgacttcaag gggaagaaat ttctacaaat
     2581 attgattatc attgcttcca tgtcagctcc tttcgtagga gcctactcct ggattctctt
     2641 gctgggacga aatgaggtca tcactaaatt tttgacaaat gccctttatc ttccagctat
     2701 cgatatttat ggattcaaag gaattgtact tgtctttaca ctgcaactat tcccactggt
     2761 atttctatat gttgctggaa caatgaacag tattgacaat tctctacttg aagcagctga
     2821 aagcatgggg tccttcggat ttaaacctat cgtaacggtt gttttacctc tcctagttcc
     2881 aaccttacta gcagctcctt gcttgtattt atgagagcat tctcagactt tggaacgcct
     2941 atgttgattg gcgaagggta tcggactttc cctgtcctga tttataccca atttattagc
     3001 gaggttggag gaaattctgc ttttgcaatt atggcgatta tcattgcctt ggcaattttc
     3061 cttatccaaa aacacattgc aaaccgctac agtttcagca tgaatctgct ccatccaatt
     3121 gagcctaaaa aaactacaaa aggaaaaatg gctgccattt atgcaacagt ctacggaatt
     3181 atctttatct ctgttttacc tcaaatctac ttaatttata cctctttcct aaaaacatca
     3241 ggtatggtat ttgttaaagg ttattctcca aacagttaca agctagcttt caatcgtatg
     3301 ggatctgcta ttttcaatac cattcgtatc cctttgattg ccttagttct agttgttcta
     3361 tttacgacat ttatctccta cctagccgtt agaaaacgga atttgtttac aaacttaatt
     3421 gacagcctca gtatggtacc ttatattgta ccaggaaccg ttctagggat tgccttcatt
     3481 tctctggtct atttggaagt ggatttctta tgattacagg gactgctttc atcttgatta
     3541 tgtctctatc tgccagaaaa ttaccttata ctattcgctc atctgttgct agcttacaac
     3601 aaatagcacc aagtattgaa gaagctgctg aaagcttagg aagtagtcgt ctcaatatct
     3661 ttgctaagat tacaactcca atgatgctat cggatattat ttctggagcc atcctatctt
     3721 gggtcacact gatttcagaa ctctctactt ctatcctcct ctacaatgtc aaaacaagaa
     3781 caatgactgt agctatttat acagaggttc tcagaggaaa ttacggtgta gctgcagcct
     3841 tgtcaactat cctgactgtt ctaacagtag gttccttgct cttgtttatg aaaatctcta
     3901 aaagcaatag cattacactt tagttttcca catcaaaaac agccgaaagg agtaccctcg
     3961 gctgtttttt cttatcttga tattcttatc aaatccctag ctcatggcaa gcaagtctag
     4021 actgattaaa tagagccttc ttccatcttc tcacgtccta gctctcggta actacggtca
     4081 ccgacaactt caaccctaaa ctgaccgtcc tcatattcaa gaatcgtcac gctaccatta
     4141 tctagaccat gcggatgcat gccattaatc agataaacaa tggttccaat agtcattcca
     4201 tggctgacaa caagggcgtt acctccacct tgatcttcca tttcttttgc aatcatttca
     4261 aagccttcct tgattcggcc actgagtttt tcccagcctt cagcccaacc agctgtatcg
     4321 acctctacca agccctcagc cagttcagca taagacaatt ggtgaacgtg gtccacatta
     4381 aagatacgag gaataatgcc catgaaaaga tcgccatcat aggctccatc aaaactaccg
     4441 aaacaccatt ctctgatacg cttgtccatg cgataaggga ttttcccctg caagccaagt
     4501 tcttcaagga taattcccat ggtctgaatg gtacgaccag aatcactcga ataagcgcgc
     4561 tcaaactgta gatcagattc tcgcaaaccg attcctaact cttgaatccc tcgttcacct
     4621 tcagcagtta agggagtatc gctccaacct tgcgcgcgac caatcgtgtt aaacatggtc
     4681 ttgccatgac gtaccaaata caatcgtact tttaccattt tccgtcctcc gtttgcttct
     4741 attatatcat gaatgataaa ataaaagcca cccatacagg cgacttttga aggagattat
     4801 tatgaaaaag tttaggagtt cattaaataa agatattaga tgaaaatcaa attcaaacta
     4861 attcagtgtt atctgtttca aataatatta gg
//
LOCUS       DAVKHZ010000100         4888 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000100 DAVKHZ010000000
VERSION     DAVKHZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.100"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(65..445)
                     /locus_tag="VWW99_001928"
     CDS             complement(65..445)
                     /locus_tag="VWW99_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU9539719.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVGL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFA"
     gene            complement(591..1943)
                     /locus_tag="VWW99_001929"
     CDS             complement(591..1943)
                     /locus_tag="VWW99_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU9539720.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLVKLRAKEAIFEKFEQSLNEKYNRMSNSGEEQEYYGKVEYYLSQLNSENYNN
                     STQYSKIQDEYTKLNKITAERNQLDADLQTLQNELLQLQQQGDSPSLSDTTSADDKAK
                     LETKILEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLVPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(1940..2407)
                     /locus_tag="VWW99_001930"
     CDS             complement(1940..2407)
                     /locus_tag="VWW99_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9539721.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(2400..4619)
                     /locus_tag="VWW99_001931"
     CDS             complement(2400..4619)
                     /locus_tag="VWW99_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9539722.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVVDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYENMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIVEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
BASE COUNT         1581 a          984 c          672 g         1651 t
ORIGIN      
        1 acatatatac aataaataac ttacaaagtt tatttcacca attcctgcaa aaaaatgttt
       61 cattttaagc aaaaatataa ccatctaagc taccgtcagc ccaatcagcc ccatatgcga
      121 ttgctccacc tagtgaaaat ccagtaacag ttagtgcaac gtttagtgca gtattgacta
      181 aatggccagc tgcactacct acatgtttaa ttattaaact acgtaatgtt ctcgtaatag
      241 cccctctatt agctgagata gcttttttta tcgcataagc agaataaatt gttgcaccac
      301 cactatagat ggccaaacct acatccagaa ctgttgcaaa actacctcta ctattccacc
      361 agttacgacc aacaccaccc ccatcaagat acatcatctc ttcttgctca agagcaacat
      421 aattatttgg taatacgagt tccatttatt ttctcctttt ttattaagca tggttaggat
      481 agtttcctaa caagtgagca tcgactttgt ttatagcact tagttaccta gataagatat
      541 cttcccttta tcttacttta ttttataggt gacttaatgt ttaaaaccaa ttattgcttg
      601 aaacttaaca tttccaaaat ccaatcaaga taggtttctt tatcatagac aatccgcact
      661 tcaactggca tatccttttg gaggacgact gtctcacttc catgttttag agttaaggtt
      721 tctaattcta tcatgacttt ataaagacta atattgccct ctttcgtttc ttgggaaatt
      781 gttcctgaat caatctgtct gacttgtccc tttagtgttc catatttttg actatttaca
      841 ccagtaatag caacatcagc ttttgctcct ttcgaaacac gagaaatatc actcgcaagt
      901 acaaaagctt ctacatagta acctttttct ttccctgaaa cttctgctat cgtttgcccc
      961 tgctgaatag acattccttg tttcaaaggt accaaataat gcagagtacc ttcatttggc
     1021 gctaatatac tatgagcttt atcttgacct gttgccactc caagattagc ctcaagctct
     1081 gtaatacttt tattgttgtt tgaacgagca gtacctaact cactaactaa ttgagcataa
     1141 atattataag cctgagaagt tggatcatta taggtacggt tcatatcttt aatattgctt
     1201 tgttgactat caatctcgct attcttagaa gtaatatttg tttttaatgc ttcgattttt
     1261 gttgttattt ctaatatctt agtttctaac ttagctttat catcagctga tgtcgtatct
     1321 gataaggaag gggagtctcc ttgctgttga agttgaagca attcattttg tagagtttgc
     1381 aagtcggcat ctaactgatt tctttcagct gttattttat tcaacttcgt atattcatcc
     1441 tgaatctttg aatactgggt actattatta taattttccg aatttaactg agataggtag
     1501 tattcaactt tcccataata ttcctgttct tcaccagaat tagacatacg gttgtatttc
     1561 tcatttaatg attgttcaaa tttttcaaag atagcttctt ttgcacgtaa cttcaccaat
     1621 tgtttattta aagagctcgc ttgtaaacct tcttgtccac ttgataacga aataatgaca
     1681 tctccagcat ttaccatatc tccttctgac ttatgtaaag taactacctt ccctgaacca
     1741 attgctgata ggaactctgt acctgttata actgaatttc cattcgcttt tacaatatag
     1801 tttttgggta tataagctgc gccaaccaat gcaccgctta agataatagc agttgaaata
     1861 atgagaataa acgcaaaagc tggtggtctc ttatcaaaga aaatacgaga ataacgtaat
     1921 tctgatttat tatataattt cataggctta caattggtct aaaaatatct actaccattt
     1981 tttcaggaga agaattaaca taaactgtat agacaatccc atccgtttga atatcatttt
     2041 catagacata tagatccaat ttagaatacg catactgtag atactctgga ctgtcttcaa
     2101 aacgaacata taaacaatat ggaacagaga tagaatcctg tacatcataa atgttactgt
     2161 actgttgagc attatgagct tgaatataaa actcaaaatc agtcgttatt aatccatcat
     2221 catgaatagt agtaccacaa ctttttacaa ttaatggacc aaaaatttgt gcttttaaca
     2281 actgcaaatg ttgatgaaat ttattaattt cctgatctac atcttctact tttgtatcat
     2341 gtaacttttt acagataact gactttagta ccagtttttt attatctttt acctctaact
     2401 tagccataag taacctcctc tgtatctaac acagcctgtg actgaatttg ttgattcact
     2461 tgaacgctct gcaaaccaac cattctagca tactttccat ttttcgccat tagttcttca
     2521 tgagtcccat attccacaat agtcccattt tctaaaaagc atatcttatc acatcgaaga
     2581 attgtagaca gcctgtgggc tactacaatt gttgtcttat ccattatttt attaaagatt
     2641 aaatcctgaa taatctgttc actaaatgaa tctaagttag aggttgcctc atcaaatata
     2701 tacaaatcag ctttactcag tagtgctctt gcaatagcca atcgttgttt ctctccaccc
     2761 gagaaatttt gaccgccttc ttctacaaag gagccataac gattttgaag acgttgaatc
     2821 gtatcatgaa taccaacaat acgacaaact ctcaccatat tctcatatgt aacagaagga
     2881 ttaccaattt ttagattatc aataattgtc cctgtaaaga gttctatggt ctgaggaatg
     2941 taggaaattt tcttacgtag tttaccatag tcaagatctg ataagtcatt ttctcctata
     3001 cgaattttcc cctcactcgg atttataaat cttaataaca aacgaactag cgtacttttt
     3061 cctgctccgc tttctccaac aaaagctatt ttttctcctt gtttaattcg caaattaaag
     3121 tccttgataa caggagggcg tgaaccatag gcaaatgtta catctttaaa ttcaatgtca
     3181 tcacataagg tgaagtctct aatactataa tccaaggcaa tgtcttcacg atctacttcc
     3241 atcaattctt gaagccgact aaccgcaacc tgtacctctt gaaaggttaa ctgcaagcca
     3301 actaaattct gaataggttc tgtaaaatat tgacttaatg tctgaaaaac caagaggtcc
     3361 ccaatagtca tttttccatc aatgataaat aaggcgccca ctcccataaa taataaccca
     3421 cccatggttg aggtcattga agatattgta gattgaatat tctgcaaaac accctcttta
     3481 taaccaattt ctaaagtgtg aacaaatttt tcttcaattt tgtttaaacg ttgttcctca
     3541 tcatgctgag acttaatggt atcaatgtta cgaatagatt caattaactg tgaattaaga
     3601 agtccatttg cttccatttg ttcatgatta atttttttat aaggcttttt aaaacagtat
     3661 ataagaatga tgttaacaat aaccataaaa actaatatca gaaataaaga ttgattaata
     3721 gtccaaagga caaccgcact aataactgac aaggtgatat ccacgactaa tgaaatagag
     3781 actgacgtaa acacattctt tatcgtcatc gcatcttgaa atcttgttaa aacatcacca
     3841 actctacgac ttccaaaaaa actataagga agatgaataa tatggtcata atatcccatt
     3901 aaaaccggta tatcaatttt tcgagataga aacaaaagaa cgtgctgacg aaatgcagat
     3961 agaagagtct gtaagaacga cactattcca aatactatca aaaacatata cagactattt
     4021 tttaaagcat atggaatcac ctcgtccatg ataacttttg aaaagagact cgataaaata
     4081 ccaatgatac ttaaaacaaa ggaagctaat atgactgtac taatcaatcc tttttgatta
     4141 aaaattaacc ttccaaataa atcaagaagt ccttttccct ttagtttccc tctttcaaaa
     4201 tccgaatttg gaaccataaa aagagtaata ccttgataaa tttctcccag ttcgtcactt
     4261 gacatcttgc gtatgccact agcagggtct gctacgtaga acttactatt ccttttgata
     4321 ctatgtaata ccacaaaatg attttgacct aaatcattct taacttgaag aatcgcagga
     4381 aatgttaatt ttggagtcaa atcttccagt gctactcgaa cagcttttac tgtaaaattc
     4441 aacttattca gacctgaaac aataccactg acagtcgttc catacatgtc tgtaccaata
     4501 atttccctaa tcttcatgat tgatagttcc tttttgtaag ataaaagaac tgttgaaaca
     4561 actgccgcag cacaatctga ctggtcatgt tgcatcgtac aaggatattt ttttaacata
     4621 gaaatactca attctttaga attaatctaa cttttctttg atgtcaccca aacctcaatc
     4681 actgcccagt agttcttgaa ttaatttatt ttcaaccgta tcattctttg ttcttgatat
     4741 ctttccaagc attgtagtac agcagatttt tgcccaagga ctgagcctcc tttaacaact
     4801 caaactccct tacattatat cggccagccc catggaaaga ttatagccag actaatcaat
     4861 aatataatac atactagaat acaaaata
//
LOCUS       DAVKHZ010000101         4570 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000101 DAVKHZ010000000
VERSION     DAVKHZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.101"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            11..739
                     /locus_tag="VWW99_001932"
     CDS             11..739
                     /locus_tag="VWW99_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9539723.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1199..1393
                     /locus_tag="VWW99_001933"
     CDS             1199..1393
                     /locus_tag="VWW99_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9539724.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(1759..3069)
                     /locus_tag="VWW99_001934"
     CDS             complement(1759..3069)
                     /locus_tag="VWW99_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9539725.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            3306..4178
                     /locus_tag="VWW99_001935"
     CDS             3306..4178
                     /locus_tag="VWW99_001935"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9539726.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            4309..4570
                     /locus_tag="VWW99_001936"
                     /pseudo
     CDS             4309..4570
                     /locus_tag="VWW99_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1464 a          739 c          955 g         1412 t
ORIGIN      
        1 accagattaa atgagtacaa aatatttatt tatttacaat gagattcgtg aaaagattct
       61 ttgtaataaa tataccatga acgaacaatt acctgatgaa atgacattag ctaaacagtt
      121 tgcctgtagt cgaatgacga tcaaaaaagc tttagacttg ttagtttctg agggcttaat
      181 ttttagaaaa cgtgggcagg gaacctttgt tctctctcgt ggcagctcaa aaagaaaatt
      241 aatcgttcca gaaagagata tccggggact gacaaaaata tctgaagatg ctcattctac
      301 aattgactcg aggattattc acttcaaatt agaatttgca aatgaatttt tagcagaaaa
      361 actacaggtc gctttgcaga gtccagttta taatatttac cgcctgcgta ttattgacgg
      421 taaaccttat gttctggaac aaacttatat gagtaccgat gttattccag gtattactga
      481 agatatttta caaaaatcga tttacaatta cattgaagga aagttaggat tgcatattgc
      541 cagtgctaca aaaatcttac gagcttcttc tagttcagaa aatgagcaac attacttgca
      601 gctccttcca acggaaccgg tatttgaagt agaacaagtg gcttatttgg ataacggaac
      661 tccgtttgag tactcgatta gtcgtcatcg ctatgattta tttgaattta attcttttgc
      721 attacgacat tcctcctagg agaaaatgtg aaaatgaagc caatctttta cagactctag
      781 tttaagaaaa atttaaaaca gggcaagaag gtcccatcta tgcttaaatg gtttctcttt
      841 tctaaataag atggctttaa aagagtgatc gttttatcca tcatgttgaa aaatatcttc
      901 gtatagctta tagagtaggt actgaaattg ttcacctgat ctacttctta tagttattta
      961 gttttaaata gtgtttcaaa tattcttaca ctgacgagaa gtttttgagt cttttcttgt
     1021 aacacatata gtatactgag gttagaatag tagactgtga cttctaacaa attgctagaa
     1081 atgaatttca atctcccaat ttatttgttc aaatattgtt ttaatatatt gaataaattc
     1141 tgaatcataa tcatttaaaa aaattttatt tttttatttt tcattacgaa taatatagat
     1201 gaaggggaaa gagtatgaaa acagaactgt ttcttttgct attagttcaa aaggagaaaa
     1261 aatgaaagta gaaaatattt cgtatagggt ggatcatcgt atattgtttg ataatatttc
     1321 ttttgatact tcgagttcag gcgtgacatt aattactggt aaaaatggta caggaaagtc
     1381 aactttacta tagtagattg aaactagaat agtacacatc tacttctaaa atattgttag
     1441 aaatcgattt gactatcctg atctatttgt cctgttctta tttcatttca ctatatctca
     1501 aattgagtat gacgaagtgc gctcccatgt cctgggaacg cactttcttc atatttttca
     1561 tattcttgaa tccatcgata aagactattg ggatgaattt ttaaagttga actaatcatt
     1621 tttacaggat gagatttaca gcagagagtt tgaaggcttt atcaaaggtt tttcttggca
     1681 taatgacttt tcctcgtttc cacttaattt tgtgtctact ttattatacc aagtccaccc
     1741 ttaagttaga taataaatct aacttaagga agctagaagg atgagaatcc aggtggtcaa
     1801 gagtcccaaa cttaagctga tggggacacc cagaataatt tgctttttga aggcaaggcc
     1861 acgttcctct atattgggaa gtgagagttg aatgagagaa ccagctgatg aaaagggtga
     1921 gatattagta gatagagcgc caataacggt ggctgttgtg agtaagtgaa tatcaatctg
     1981 aggattttga gcactgatga tagcaatgat gggaaagagg gctggagcta caacggatag
     2041 ggtggaacta aagagtgaca tcactccggc tatcacacaa aagaacagag gtaaccagaa
     2101 atgaggaatg gttgttgtca tgaggtgccc tatcagtgtg actaaacctg acttgaccgc
     2161 tagagacatt agtaagctca tgccgcagag catgataatt gtagcccagg gaaccttagc
     2221 taaaatggct tcttgcttcc ctaatttgag ccttaaggcg aggcagacca tgagtattga
     2281 gacaaagcca atatcaaatg ttttttgata agtagctatc caggcgatgt ttgggaaaat
     2341 gagatgcaac aagggaaaaa gccaaaccaa aaccatgctg ctgatcatga gcaaggtggt
     2401 ttgtctttga accttgctga ggagtggtgg ttggtcaata gtcaaggatg agtttgttct
     2461 tcccttacta tagtggctgt aacaggataa taaaagcaag acgatgagtg ggtagataat
     2521 gctgacgata aagatatgat tgccaagtga aaaagcttgc tcttcccatc ccatttgctt
     2581 aaacaggcct tgaaagacaa tgcctgagct actggttatc aaattagccc ctcctgaagc
     2641 tccccaattg acggcttgag ctccaatcaa agggtgtttg tccgcttttt gacagagggt
     2701 aatcgctaga ggacagcaaa cggccatagt agtgaaaaat ccagcaccta aagcagacaa
     2761 aagggttgcc atcaggtata aaatcatgta gagggcgtta gggtgggtgc gtgtgcggta
     2821 gagaatgtgt tgagccaaaa catcaagagt accgttagtt gttgcaacgt tataaaagag
     2881 agagacgcta aaaatggtaa aaaagagtga ggttggccaa aaatgaagaa gttctttggg
     2941 gcttaatccc atgagagtgg ttgcgatgag gtaagaaaaa gcaatagcca gcaggccaat
     3001 attgattttg gtgcggtaac caattccaat ggctagagca atggcgctaa tcattattaa
     3061 atgaatcatt gaattgtcct ttcagttaga atatggaaat agattggcaa ttttggcgct
     3121 caaaagacca cctgtttcct attggtactt gactattata cacaaaaaaa atacaattgt
     3181 cttgacaatt gtattgacac ttgttagaat taaaaatgag gtattcaggc ttgtgatttc
     3241 ctatggaagt taatagtaat tgcctctagt gcttacaagt gatattaaaa atagaggact
     3301 tagtgatgtc aatcatttca actgatttaa ccccttttca aatagatgat acattgaaag
     3361 cagccttgcg agaagatgtt cattccgaag attacagtac caatgccatt tttgatcatc
     3421 atggccaagc caaggtgtcg ctttttgcca aggaagctgg tgttttagcg ggattaaccg
     3481 tttttcaaag ggtttttacc ctatttgatg ccgaggtgac cttccagaat cctcatcaat
     3541 ttaaggatgg ggatcgtttg actagtggcg atttggtttt agaaatcata ggctcggtga
     3601 gaagtctctt aacatgtgaa cgcgttgcct tgaatttttt acaacattta tcagggattg
     3661 cttcgatgac agctgcttat gtagaagcct taggcgatga ttgcattaag gtatttgata
     3721 ctcgaaaaac tactcctaat ttacgtcttt ttgagaaata tgccgtgaga gttggcggtg
     3781 gctataatca tcgctttaat ttatcagatg ctatcatgct aaaagacaat cacattgcgg
     3841 cagtaggtag tgttcaaaag gcaattgctc aagcgcgtgc ctatgcccct tttgtgaaaa
     3901 tggtcgaggt ggaagtggaa agccttgctg ctgccgaaga agctgcggcg gcgggtgctg
     3961 atattatcat gttggataat atgtcattgg aacagattga acaggctatt accctaattg
     4021 caggacgttc tcggattgaa tgttctggaa atattgatat gaccactatt agccgttttc
     4081 gtggtttagc gattgattac gtctccagtg gtagtttaac ccatagtgct aagagtcttg
     4141 atttttccat gaaggggtta acctaccttg atgtctaagt tgtaaaataa actaactttt
     4201 taaaggatgt ttttcctcta gaacgagttt tatgtcagat agtttaaacg cctcttcaaa
     4261 tatagtaaaa tgaaccaaaa atagtacaca atgtggtata atctttttat ggcatattca
     4321 atagattttc gtaaaaaagt tctctcttat tgtgagcaaa caggtagtgt aacagaagca
     4381 taacacgttt tccaaatctc acgtaatacc atttatggct ggttaaagct aaaagagaaa
     4441 acaggagagc taaaccacca agtaaaagga acaaaaccaa gaaaagttga tagagataga
     4501 cttaaaaact atcttactga caatccagat gcttatttga ttgaaatagc ttctgaattt
     4561 ggctgtcatc
//
LOCUS       DAVKHZ010000102         4394 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000102 DAVKHZ010000000
VERSION     DAVKHZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.102"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            104..328
                     /locus_tag="VWW99_001937"
     CDS             104..328
                     /locus_tag="VWW99_001937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539727.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDGTDVVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFEEIFYEYKSKTFVNRDTL"
     gene            complement(640..801)
                     /locus_tag="VWW99_001938"
     CDS             complement(640..801)
                     /locus_tag="VWW99_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9539728.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
     gene            complement(786..1004)
                     /locus_tag="VWW99_001939"
     CDS             complement(786..1004)
                     /locus_tag="VWW99_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9539729.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(1102..1887)
                     /locus_tag="VWW99_001940"
     CDS             complement(1102..1887)
                     /locus_tag="VWW99_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539730.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1889..2707)
                     /locus_tag="VWW99_001941"
     CDS             complement(1889..2707)
                     /locus_tag="VWW99_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9539731.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2709..3701)
                     /locus_tag="VWW99_001942"
     CDS             complement(2709..3701)
                     /locus_tag="VWW99_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9539732.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1479 a          936 c          754 g         1225 t
ORIGIN      
        1 tctccataaa aacagtatat catttaaaaa aatttaattc aagaaaaatt aacatgcatt
       61 aatttatttt ttagcacacg ttaaaaaata agattagtac tcaatgaaaa tcaaagagca
      121 aactaggaaa ctagccgcag gttgctcaaa acactgtttt gaggttgtag atggaactga
      181 cgtagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag tcagtaacca
      241 tacgcacggc aaggcgacgt tgacgtggtt tgaagagatt ttctatgagt ataagtccaa
      301 aactttcgtt aaccgggata cattataagc acattcatac catcaaaaat agtagcaata
      361 aacttgaaca actaccagaa acacgaaaac aggcataatt gatgaaatat cagcgctatt
      421 aaacaatata aataaccgct aagaataagg cttaacggtt atcttctacc tatctatttt
      481 ttcaactcct cagtcgttta tctacctata acctaggctt tacgagtgcc ttgctctacc
      541 aactgagcta aatcggcgat tacacttaca gtatggcact ctgagaatag atgtcaagta
      601 atattattca agctttttaa tcaaaatctg acaaggattt tacggattcc atagctgaga
      661 aaaaatctct aactttttaa agccaagacc tcgataaaag tcatttgttc gatcataatc
      721 tttattagaa ccttctgcca ctgttttgac ctgcagataa ccaacttttt tacgagcttc
      781 actctctaaa gtagcaagca attggctccc aatttttcta ccttgataag cttttttacg
      841 ccgagacaat caatctctgc acaatcttca ctcgaatagg ataaccttac aaatccagtc
      901 aaatcactct cctgataggc ggtccaaact tgcagtgtcg tggttccttc tatataggct
      961 tgtgtgcttt ctgggattcc aaaccattct ggcaaatcct tcaaaacctc aacgacaact
     1021 gccttttttg attttcatcc ttgacttctt caattacaaa catcataagt tcctttcttg
     1081 attttagtct tactttagct tttacgaacc cgcactttcg taggaatcta agcccttatc
     1141 ccaaagttta agggaaataa caaagaaaac cagagaaatc aacatcaaac ctcctacgtt
     1201 aaagaacaca tccttttcct gtaagaaata gctagctgga tagtaggctg taaaggcgaa
     1261 aggcacgata aagctaatca accaacgaag aagagaattg taaatagaaa tcggatactt
     1321 agcaaagtca ttgaacatat agaagatgta aatcatggcg cctgactgct tagtccaaaa
     1381 ggcgatactg gctgttgcga ttttaagaga agtataaatc aaggtcgcaa aaggaataca
     1441 aactaggaaa agcaggaatt ttggaagagt ccaaacaatg ctggtcactg ttgttcccaa
     1501 taaaatacca ccgactaaga gttcacccaa ggcatcaatc tgaaaggttt caactaggat
     1561 gtgaaagaga ggattgatgg gacgagtcag atacttgtca aactctcctt ttcggactag
     1621 gcgttgccct agtgcccaga gattgtcaaa aaagagatgg tccattccct tgggaatcaa
     1681 ggaaaatcca taaatgaaag ctatctcttg aaaggtccag ccttctagga atggaatatg
     1741 ttgaaagatg acattgagaa acaagagatt caagccttga gccagaaaga ctcccaagac
     1801 accaaccaca aaatccacct tatattccat gatttgtttg atgtattgtc tgataaaaat
     1861 cagatgcatt cgttgatatt ttttcatact aacctccttg aatggtgata aaggactgga
     1921 cccgtttcca aattaactga gacaatccca ccatcactaa gagccagaag aactgcaaaa
     1981 ggagtgcctg aagaatctga ctggcatcgt attttccaac aatgatcata actggagtat
     2041 aaatcaagga cgaaaaaggc aaaaaggaga gaatatctga aacaaccttt ggaaagaatg
     2101 ccaagggaat caaactcccc gacataaaag ccactatgga agtcttaagt aggttggaac
     2161 cccaaagatt tttaaacaca aaggctgaaa atccaaagca aatattaaag aaaaagttaa
     2221 tcagataggc gagcgttaag ctaaaaagat aaaggacagt taatcctagc acctctacaa
     2281 taccttgacc cgatatgatt ttcatcaaga caatgacact taaaaatgga aggccaacgc
     2341 tgataaaaat caaccacttg gaaccaagct cggtgaaaag ataggaggcc gcaaaatgca
     2401 ctggtcgcaa caaacgcatg ataatggagc catccttgac ctcctcccca atcataaagg
     2461 acgaatcgga tctagtcaga agattggtca caaaactcat gatgatgtag agggtgatat
     2521 ccgccatact gaagccctga atcaaagact cttgcgaaga atcaaagaca gccttccaga
     2581 gataaaaggc cacaaaagcc cccatgacat cgccaatccg atagagaata aagttgactc
     2641 ggtaagtaat caactcctga acccctgcat tgataaaggg tttataacgt ctccacaatt
     2701 tgatcatcct agagctcctt tcggtagaag cgacggataa tatcctcaat atccgtatcc
     2761 accatcttca aatcgcggat ttcaaaatca gatagggttt gcttgataat gtcagctgac
     2821 tggtagcgag aactatcaaa ttcaatgttg aggctgtttc cttgtctatc aatggtcata
     2881 tcagacagac cttcatagtg agagacgaga tgactttgac ctggtagcag ttcaaaagag
     2941 agagtcttca tcttaccaaa ggtctccttg agttggctca ccgttccatc aaaaatcttt
     3001 tgccccttgt caatcatgaa aatccgatca caaagttgct caatatcact caaatcgtga
     3061 gtggtcaaaa gaatggtagt ttcttcctct tgattgatct gagtaattgc ccgacgaata
     3121 ttatccttaa ccgaaacgtc caaaccaatg gtcggctcat ctaaaaaaag aaccttggga
     3181 ttgtggagca aggaggccgc aatatccgct cgcatccgtt gtcccagtga aagagtccgc
     3241 acgggatcct tgataaagtc cttcaaatcc aagacttcat tcaaaaagtc catacgctta
     3301 tgaaagagcg agtttggcac atcataaatc tcttttaaga cagtgtaggt ctcttgcaga
     3361 gccaaatccc accatagctg ggtgcgttgt ccaaagacta cgccaatatc tttgacataa
     3421 tcttgccgat tgtcctgggg aatcttgccg ttaatccgac aaaaaccaga tgttggtttc
     3481 aaaattcctg tcagcatttt aatggttgtc gacttcccag caccatttgc cccgataaat
     3541 cctaaaatct gcccttttgg aacctcaaag gtcaaatcct tgaccgcttc aaaggtctgc
     3601 ttttcaggat gaataaagga gcgcaaagcc cccttcaagc ccggttcctt aacaatcttc
     3661 acaaaatgtt tctgaagatg ttccacttct atcattgcca tatctatctc cctatcgtaa
     3721 ggaatagcaa cattgtgttt ttaactacaa atattctaaa tccttacttt ctacaccttc
     3781 tacattttat tactacagaa ggctttatac caacctatta tagtccaata aaaaacgaaa
     3841 tgcaagcgtt tgcctataga ttgtgaaatt aggatttctc tcatgaaata atacttttaa
     3901 tcaaaaattc tgtttaatag tttaaattac caacctactc atcttctcac cagagggcgg
     3961 tattaacttt gatcataaaa tcggatactc cctctatcca gaaaatatgc tcagaatgat
     4021 tgtctctaaa caatacctgc tcttttacct cattcatgaa aaagaagtcc ataccctgag
     4081 aattatcaac tcacgcaccg actacctaaa ccaactcgac cacctctttc gtacatgtag
     4141 gaaactattt tcctcacaaa taatctcatt ataaaatcca ataaattttt ttagctcact
     4201 tgaatatttt ctttgtatcg ttttatcagc ttcaatctga tctagacttg ttaccttgga
     4261 acgacttgaa ataacctttg taaaataaca cttaaggctt tcatcatctc gtataaattt
     4321 ttcaatagtg atataaaaag ctttcatatt ctgtttgtaa tcagatatta attgattaat
     4381 tttatcattc tgat
//
LOCUS       DAVKHZ010000103         4282 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000103 DAVKHZ010000000
VERSION     DAVKHZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.103"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..246
                     /locus_tag="VWW99_001943"
     CDS             <1..246
                     /locus_tag="VWW99_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539733.1"
                     /translation="GIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVV
                     INEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            560..1537
                     /locus_tag="VWW99_001944"
     CDS             560..1537
                     /locus_tag="VWW99_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539734.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     SGIAVFYKESQTTNNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLNKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            2008..2382
                     /locus_tag="VWW99_001945"
     CDS             2008..2382
                     /locus_tag="VWW99_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9539735.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            2590..3492
                     /locus_tag="VWW99_001946"
     CDS             2590..3492
                     /locus_tag="VWW99_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539736.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEMSISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            3548..3901
                     /locus_tag="VWW99_001947"
     CDS             3548..3901
                     /locus_tag="VWW99_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9539737.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGFGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            3898..>4282
                     /locus_tag="VWW99_001948"
     CDS             3898..>4282
                     /locus_tag="VWW99_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539738.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQS"
BASE COUNT         1423 a          554 c          770 g         1535 t
ORIGIN      
        1 ggaatcattt acgataatga agcattaaga gatttgtcaa aacaatattt aaacaaaaat
       61 atttcggaat tacaagatgg ttctttcgag ggcggtatac aagatgctgt tgcttctaaa
      121 caaatagtag ttataaatga atttatagct tgtttaaatt ccagatgcgt gccatcccaa
      181 tatgatagat ttttttataa agatagacct tatatttttt ccagaaagag tcctattaag
      241 gggtgaaaat ttgttacatt tggattaaca agtgtcctag ggattagaaa taactctagt
      301 atagctagat atttttgagt aactagaaat atcattaggc tacccatatg attgccagaa
      361 aagatggctg atgaatctta tttatactgt tgcagtaggc gttgtctagg ataaataagc
      421 tatcttgatg ggggagattg gaataatttt agtagaaaca ttaaaggatt atggacaagt
      481 agcgcaggat taggtgatgc aatgtgctaa gttggcttgg tgttagcagt agtattattt
      541 aacaatggaa agtagactaa tgaaaaataa gcgttatttt tttgacacaa ttttaataat
      601 tttactgctt ataagcacga tattttgtgt cagcccagta tttataaaat tggacatctt
      661 aggaactccc tctcatgcca tcttaacctt tgtattggcg ataccgttat tctatatttt
      721 gtctcaatgc ttacatactt tgctattact agtttcctca atcttttgta aattaagacc
      781 aatttatttt tattttatat ttgtgatcat tataggagct aggaaatact atcgaattct
      841 ctttcaccaa ctaatgggat ttagttcagg aatagcagtg ttttataagg agagtcaaac
      901 aactaacaat ctgtttaaat tttactactt cttgtacttt acaacactaa tttcatacta
      961 ttttttcttt acttttgtgt atgacaaacc gttgcttttg cctcttattc cgttttctat
     1021 tatcatagca ttagtacaaa aactttatag aattgagaat caacaattat ttctgttaaa
     1081 atcaaaggta ctaacaatac tggaatctaa gagggattgt gagtttaatc ttcaggatta
     1141 tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca tgcgtggctt tatcttatat
     1201 cagtttgatc aagccatatc tttcagagag cgtgcgtgaa caaattgatt tattagaagt
     1261 taagcgattt aaaaaaataa atcatccaat atcactttat ggtatgttgg atgttataaa
     1321 attaaatcta tatttacggc attataatga aaaaaataaa tatgaatcaa tgttaaataa
     1381 aattttagag gtaagaccag actttgtttt aatcgagcag aatattgatg atagtttaaa
     1441 tagctcgcaa ccattatctt tatctttagc catttcagaa atccaacttt tgcttgaagt
     1501 gtatatgggg atcaaacatg tttcaattag gagataaaac tagaataaca tttgctatta
     1561 taggggcaag ttgcatatta gtcagtatgt atcttattcc acttattaaa actgagaata
     1621 aattttggga aatactttta tttatattag gtgtattgct aattggaatt gcgttactaa
     1681 gtcaaaaaaa ataaattttg tgttagtgaa tacgtaatta ttactattga ctgttttatg
     1741 gtggaaagca tccatagctt tttgtaaaat aatctgattg gctaatattt gcagtaggtg
     1801 ttgtctatga taaagaaacc tatcttgatt ggattttaga actgttaagt ttcaagcaat
     1861 aattggtttt aaaccttagg taacctataa aaacaaataa ggtagagaaa ggatatctta
     1921 tttaggtaac taagtactat aaacacagtc gacaatcact tgttaggaaa ccatcctaac
     1981 tatgcttaat aaaaaaggag aaaataaatg gaactcgtat taccaaataa ttatgttgtt
     2041 cttgagcaag aagagatgat gtatcttgat gggggatttt ctattccgag atggcctgtt
     2101 gcaacagcca ttaatatagc ttttaatggt gttttaggtg gaggagcaat cagtctagtt
     2161 agaaattata ttcgtaatta tggtttgcgg cgagttacaa gcgcaattgc tggagcagct
     2221 gcaagatatg ttggggtacg agttgcaaat agagtggcag gatttgcact gtctgctatt
     2281 aatggatttg cagcttggat gtcaattggc gatgctatta caacaatctg ggccaataat
     2341 gatgtaaata ggagagaccc aaatttaaac gccttgtggt aactagatat gggtattaaa
     2401 atggaaaaaa tttttgttat tatttttttt gtatgtctat ttatatcatc cataactttt
     2461 ttagcctatg attttgttag cgaagaaata aaaaagttga ttatttggat gaatgtcgtt
     2521 tttttgattt taatcatagc aatgatgatt tatccaaaat taagaaaatg ataaggacac
     2581 tgtaaaatta tgatacgtaa actaattgtt ttcttactaa tgctccctct ttttgtcttg
     2641 tggctagagt tgcatgtcct agtgaataac ttactattga atctagaatc tccccttgat
     2701 tttgtgatta gtatgagtct tgctttctgt agtttgattt tatcaaaaat agttttagac
     2761 atactatacg cattgaaaga tttatacaag aaagaaggtc ttattaagat ttttcccttt
     2821 atttttataa gaaggaaaaa agtgaatgtg agattctcgc cctatttttc attccatcgt
     2881 aaaagtttgt cgcctgatga tttaagatct aggattatat ggagttttat tcttgaaatt
     2941 gctattatcc tagtatttat tttaaaaatt ccttttgcaa tcatcatgct tactacaata
     3001 tttttctgga ctataatgga tatcaatcat ctagttttta ataagacgga atttcttttt
     3061 aatcaaaata aatgggaaaa agaagattcg tttgaaagtg atttgacaaa tacgttaaag
     3121 gataaaatac aaaaatcaga tctcagttat tctgacttaa tgtctcttct tctctatgat
     3181 gcgatgaacc aatccacctt tttaacggat agtgaacttt ttgaggatat tttgaaaaaa
     3241 atcgaagatt ctcataacac ccttctgtgt acaggtcttg tcgagttact gctgtatgaa
     3301 atgtctatta gtaagaatca taattggtca gaaaagattg ataaaattag gatagagctt
     3361 atcaagatta atcaattaga ttttttctat tatacaagtt ggttaaggca aaattttgat
     3421 ttctgtatga atagagagta tcataaaatg aaatctcgga aattattact gagtaataaa
     3481 aagatagttt aagttcgtcc tatttgtaaa acaaatgaaa caatttcaac taaggaggag
     3541 aaaacaaatg gaacttgtat taccaaataa ttatgttgtg attgatgaag aagaaatgat
     3601 gtatcttgat gggggagctt atttaagcaa gcgtgcttgc caaggaattt gcgcagcttt
     3661 agctatgagt ccaggaactt ttatagcatt agctggagct gcagttttaa ccaaaaaact
     3721 aataaactat attaaggttg gaggctttgg aggctggctt attggtgcag cagcaggtgt
     3781 attggctggg gcggcaggaa gaatagctta ctgtattgga tatggtgctc ttaatagagg
     3841 ttgtgatatt agcgggaacc cttatccttg ggatggattc atatctgcga cagtaagatg
     3901 agtaatgtag ataagattcg aaaaatacac attatcgtat gttggatgta tatattttta
     3961 tcattcaggg cgattataaa tgacacggag tattttttgc ttattttttt agcttttatc
     4021 tactcaatag tatcactacc tctatattct gtgaaaaata aaatagtatc tatctgtcta
     4081 gccataaatt cgattctgtt aatgagtttc cccattttaa tcaataagtt tttcccagaa
     4141 agttttttga cttataccgt attaataagt gtttttatcc tagagttagc aatctttcat
     4201 ttaattagga aagattttga tattaaattg actaacgaat ataagaaaat tagtcagttt
     4261 agaagcaagg tgtctcaatc tc
//
LOCUS       DAVKHZ010000104         4261 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000104 DAVKHZ010000000
VERSION     DAVKHZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.104"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            246..446
                     /locus_tag="VWW99_001949"
     CDS             246..446
                     /locus_tag="VWW99_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539739.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            471..887
                     /locus_tag="VWW99_001950"
     CDS             471..887
                     /locus_tag="VWW99_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539740.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1118..1825
                     /gene="ccdA2"
                     /locus_tag="VWW99_001951"
     CDS             1118..1825
                     /gene="ccdA2"
                     /locus_tag="VWW99_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9539741.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1836..2393
                     /gene="sdbB"
                     /locus_tag="VWW99_001952"
     CDS             1836..2393
                     /gene="sdbB"
                     /locus_tag="VWW99_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9539742.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2768..4159
                     /locus_tag="VWW99_001953"
     CDS             2768..4159
                     /locus_tag="VWW99_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9539743.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT         1245 a          771 c          898 g         1347 t
ORIGIN      
        1 gatgacgagc gacttttttg aagtatggtt tcagaagttt ctcttaccaa cattaaccac
       61 accatcggtt attagagtaa aatgaaataa gaacaggaca aatcgatcag gacagtaaaa
      121 tcgaattcta acaatgtttt agaagtctat atgtactatt ctagtttcaa tataccataa
      181 ctgctcctag tcaacctgca agtcctgtcc cagctccaag ctcagctccg actcaagatc
      241 ctagagtggt agcaagcgct tcggctagct caacgagcac acaagctcaa gagcaagttg
      301 acaagtctga acttcgtgcc ttgagtcaag agttagacca acgcttgaaa gctttggcaa
      361 cagtatctga tccgaaaatc gatgcaacca aggctgtcct cctagatgct caaaaagctc
      421 cggaagatag tgccttgaca gagtaaggtc tgcatactgc agtagagtct gtgaaggctg
      481 ccttggactc tctcaaagat gtgaaagcaa atgctagcga cagcaagcct gcacaggaca
      541 agaaggatgc aaaacaagga acggaagata gtaaggattc agataagatg actgaaacaa
      601 actcagttcc ggcaggagtg attgtggtca gtctacttgc cctcctaggc gtgattgcct
      661 tctggctgat tcgccgtaag aaagagtcag aaatccagca attaagcacg gaattaatca
      721 aggttctagg acagctagat gcagaaaaag cggataaaaa agtccttgcc aaagcccaaa
      781 accttctcca agaaaccctt gatttcgtga aagaagaaaa tggctcagca gagacagaaa
      841 ctaaactagt agaggagctt aaagcaatcc ttgacaaact caagtaaaac atgatttatg
      901 aaatgtcaga cttaaaagac attttcgttc acaaaagcag cgacgagtaa tccttgtcgt
      961 tgctttttgg ctattttggg ggataagatc cgccttgact agcaaaagaa aagattggtc
     1021 caatagtgcc cgatacgatt ttgcgtagca gaaagcttac aaataaaatt gaatatagta
     1081 aaataggatt agaattatta agaaagttgg ttctttattg gaaacgatag tatttttaat
     1141 ctctgttttt ctagcaggtg ttttatcctt tttttctcct tgtatttttc ctcttctgcc
     1201 agtctatgct gggattttat tggatgatca agaaagtgca aaaagctttt ctttgtttgg
     1261 gagaaaggtt ctctggtcag gcttgattcg aacactttgc tttatcgctg gtatctctct
     1321 cattttcttt attctaggct ttggtgctgg ttactttggt catattctct atgcaaattg
     1381 gtttcgatat ggcatgggag ctattattat cattttgggt cttcaccaga tggaaatttt
     1441 tcatttgaag aaattagaag ttcaaaaaag ttttaccttt aaaaaatcag attctaatcg
     1501 ttattggtca gcttttttac ttggtattac ctttagcttt ggttggacac cttgtattgg
     1561 tccagtttta agttctgttt tagcacttgc ggcttctgga ggcaatggcg cttggcaagg
     1621 agcgatttat actctcattt acactctggg catggccctt cctttcttgg tattggcact
     1681 agcttcaggt ctagtcatgc catattttag taaaatcaag cgtcatatga tgctactaaa
     1741 gaaaattggt ggtttcctca ttgttttaat gggaattttg ttactattag gacaagtaaa
     1801 tgttctagct ggaatttttg aataaaggag gacaaatgaa aaaagtaatg tttgctggct
     1861 taagtctctt gtcattagtt gtattgatgg cctgtggtga ggaagaaact aaaaagactc
     1921 aagcagcaca acagccaaaa caacaaacga ctgtacaaca aattgctgtt ggaaaagatg
     1981 ctccagactt tacattgcaa tccatggatg gcaaagaagt taagttatct gattttaagg
     2041 gtaaaaaggt ttacttgaag ttttgggctt catggtgtgg tccatgcaag aaaagtatgc
     2101 cagagttgat ggaactagcg gcgaaaccag atcgtgattt cgaaattctt actgtcattg
     2161 caccaggaat tcaaggtgaa aaaactgttg agcaattccc acaatggttc caggaacaag
     2221 gatataagga tatcccagtt ctttatgata ccaaagcaac caccttccaa gcttatcaaa
     2281 ttcgaagcat tcctacagaa tatttaattg atagccaagg aaagattgga aagattcaat
     2341 ttggtgctat cagtaatgcg gatgcagaag cagcatttaa agaaatgaac tagaatcgat
     2401 aaaatctgat tacaagatgt agtcagattt tggtaacttc taaaactaac ttccagtttc
     2461 ccacaaccta gcttttagta tgagaaaaac gcgtgacatc agtggaaatc cgtcttagac
     2521 taacttccac tggttttctt tttcttgata tataagggtt caaaagcgaa aagactcaga
     2581 aaaaagtgca cgacaaatag ccctaaaaca gagtcgtttt agagtaactt ccagttttgg
     2641 gagagagatg gaagttactt tgagaagtta cggtagtcag atttttttag aaaaacattt
     2701 tataaatatt cacatatctc ctatatttat ggtaaaatag aatcatcagt ttattttgga
     2761 gtcaaagatg aatatattta gaacaaagaa tgttagttta gataaaacag agatgcatag
     2821 gcatttgaag ttatgggatt tgattttgct gggtatcgga gccatggtag ggacaggcgt
     2881 ctttacaatc acaggtactg cagctgcaac acttgctggc ccagccctag tgatttcaat
     2941 cgttatttct gccttgtgtg tgggattatc agccctcttt tttgcagaat ttgcctcgcg
     3001 agtacccgct acaggaggtg cctatagtta cctctatgct atcttaggag aattccctgc
     3061 ctggttggct ggttggttaa ccatgatgga gttcatgaca gccatatcag gcgtagcttc
     3121 gggttgggca gcttatttta aaggcttgct ctctcaatat gggatagccc ttcctcaggc
     3181 tttaaatggt acctttaatc ctcaagcagg gacatttgtt gatttattgc ctattcttgt
     3241 cttggtcttg gtgacttcgc ttgttttact aaatgccaag gcagccttac gctttaattc
     3301 gattctagtt attttgaaat tttccgcttt agctctcttt gttttagtag gaatttggaa
     3361 tattaagttt gataattgga gcaattttgc tccttacggc tttggacaaa tctatggagc
     3421 tagtactggt attatggctg gtgcgtcctt gatgttcttc ggttttttgg gatttgaatc
     3481 catctctatg gctgtagatg aagtcaagac tcctcaaaaa aatattccta gagggattgt
     3541 cttatcgctt tctatcgtaa ccattctcta tgccttggtg acccttgttt tgactggtgt
     3601 ggttcactat agtcatttaa atgtcgatga tgccgttgcc tttgcccttc gtagtgttgg
     3661 gattagttgg gcagccaact atgtgtcatt agtggctatc ttgaccttga ttacagtttg
     3721 tatttcgatg acctatgccc tatcgcgtat gatttacagt ttagcacgtg acggcttagt
     3781 gcctgctgcc tttaaggaac tgacgaagac tagcaaggta ccaaagaatg ctactatctt
     3841 gacaggtcta gcttcagcag tagcagcagg aatgttccca ctagccagta ttgcagcctt
     3901 tttaaatatt tgtaccttag cctacttgat catgctggct tatggtctga ttcgcttacg
     3961 gaaagaaaaa ggaatgccca aagctgggga atttaaaaca ccattggtac ctctgttacc
     4021 aattttatcg atcatcattt gtttatcctt catgttacag tataatatga acacctggat
     4081 tgctttccta gttgcgttgt tagtaggaag tattatttac ttcacttatg gctataagca
     4141 ttctaccata gaagaataaa gtgaaagtct attgagttgt tgacactcag cagattttta
     4201 tatgtgaaag aaatttcaaa ttttttctaa aaatatcttg acagattaaa ttttcaggag
     4261 t
//
LOCUS       DAVKHZ010000105         3917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000105 DAVKHZ010000000
VERSION     DAVKHZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.105"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..418
                     /locus_tag="VWW99_001954"
     CDS             <1..418
                     /locus_tag="VWW99_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9539744.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VWW99_001955"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VWW99_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9539745.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSISKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHKEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1468 a          631 c          761 g         1057 t
ORIGIN      
        1 taaaaataag gcagaccaag atagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 ggcggaggcc ttgctagaaa aagtaacaga ttctagtatt agacaaaatg ctgtggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa agaaagtcaa ccgactccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtttatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaat tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacataa agaagatggc tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact ctgactttgg gagaatttca ttttattatt
     3841 aatatataaa atttcttgac atacaactta aaaagaggtg gaatatttac tagttaatta
     3901 actggttaat taattta
//
LOCUS       DAVKHZ010000106         3867 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000106 DAVKHZ010000000
VERSION     DAVKHZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.106"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            153..380
                     /locus_tag="VWW99_001956"
     CDS             153..380
                     /locus_tag="VWW99_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539746.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            668..3580
                     /gene="nanA"
                     /locus_tag="VWW99_001957"
     CDS             668..3580
                     /gene="nanA"
                     /locus_tag="VWW99_001957"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9539747.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNDQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEII
                     GIAKGSIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKG
                     SDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1330 a          640 c          866 g         1031 t
ORIGIN      
        1 aaaaatgagg tataataaat ttattaacac ttataaaaag tgatagaatc tatctttatg
       61 tatatttaaa gatagattgc tgtaaaaata gtagtagcta tgcgaaataa cagatagaga
      121 gaagggattg aagcttagaa aaggggaata atatgatatt taaggcattc aagacaaaaa
      181 agcagagaaa aagacaagtt gaactacttt tgacagtttt tttcgacagt tttctgattg
      241 atttatttct tcacttattt gggattgtcc cctttaagct ggataagatt ctgattgtga
      301 gcttgattat atttcccatt atttctacaa gtatttatgc ttatgaaaag ctatttgaaa
      361 aagtgttcga taaggattga gcaggaagta tggtgtaaat agcataagct gatgtccatc
      421 atttgcttat aaagagatat tttagtttaa ttgcagcggt gtcctggtag ataaactaga
      481 ttggcaggag tctgattgga gaaaggagag gggaaatttg gcaccaattt gagatagttt
      541 gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa gaaagttaat aaaagacaaa
      601 ctaagtgcat tttctggaat aaatgtctta tttcagaaat cgggatatag atatagagag
      661 gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt tatagcatta ggaaactatc
      721 ggtaggagcg gtttctatga ttgtaggagc agtggtattt ggaacgtctc ctgttttagc
      781 tcaagaaggg gcaagtgagc aacctctggc aaatgaaact caactttcgg gggagagctc
      841 aaccctaact gatacagaaa agagccagcc ttcttcagag actgaacttt ctggcaataa
      901 gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt ccaagagatt actatgcacg
      961 agatttggaa aatgtcgaaa cagtgataga aaaagaagat gttgaaacca atgcttcaaa
     1021 tgatcagaga gttgatttat caagtgaact agataaacta aagaaacttg aaaacgcaac
     1081 agttcacatg gagtttaagc cagatgccaa ggccccagca ttctataatc tcttttctgt
     1141 gtcaagtgct actaaaaaag atgagtactt cactatggca gtttacaata atactgctac
     1201 tctagagggg cgtggttcgg atgggaaaca gttttacaat aattacaacg atgcaccctt
     1261 aaaagttaaa ccaggtcagt ggaattctgt gactttcaca gttgaaaaac cgacagcaga
     1321 attgccaaat ggtcgagttc gcctctacgt aaacggggta ttatctcgaa caagtctgaa
     1381 atctggcaat ttcattaaag atatgccaga tgtaacgcat gtgcaaatcg gagcaaccaa
     1441 gcgtgccaac aatacggttt gggggtcaaa tctacagatt cggaatctca ctgtgtataa
     1501 tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa ctttttaaac gctcagattt
     1561 agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa acggacatat tcgaaagcgg
     1621 gcgtaacggt aaaccaaata aagatggaat caagagttat cgtattccag cacttctcaa
     1681 gacagataaa ggaactttga tcgcaggtgc agatgaacgc cgtctccatt cgagtgactg
     1741 gggtgatatc ggtatggtca tcagacgtag tgaagataat ggtaaaactt ggggtgaccg
     1801 agtaaccatt accaacttac gtgacaatcc aaaagcttct gacccatcga tcggttcacc
     1861 agtgaatatc gatatggtgt tggttcaaga tcctgaaacc aaacgaatct tttctatcta
     1921 tgacatgttc ccagaaggga agggaatctt tggaatgtct tcacaaaaag aagaagccta
     1981 caaaaaaatc gatggaaaaa cctatcaaat cctctatcgt gaaggagaaa agggagctta
     2041 taccattcga gaaaatggta ctgtctatac accagatggt aaggcgacag actatcgcgt
     2101 tgttgtagat cctgttaaac cagcctatag cgacaagggt gatctataca agggtgacca
     2161 attactagga aatatctact tcacaacaaa caaaacttct ccatttagaa ttgccaagga
     2221 tagctatcta tggatgtcct acagtgatga cgacgggaag acatggtcag cgcctcaaga
     2281 tattactccg atggtcaaag ccgattggat gaaattcttg ggtgtaggtc ctggaacagg
     2341 aattgtactt cggaatgggc ctcacaaggg acggattttg ataccggttt atacgactaa
     2401 taatgtatct cacttaaatg gctcgcaatc ttctcgtgtc atctattcag atgatcatgg
     2461 aaaaacttgg catgctggag aagcggtcaa cgataaccgt caggtagacg gtcaaaagat
     2521 ccactcttct acgatgaaca atgaacgtgc gcaaaataca gaatcaacgg tggtacaact
     2581 aaacaatgga gatgttaaac tctttatgcg tggtttgact ggagatcttc aggttgctac
     2641 aagtaaagac ggaggagtga cttgggagaa ggatatcaaa cgttatccac aggttaaaga
     2701 tgtctatgtt caaatgtctg ctatccatac gatgcacgaa ggaaaagaat acatcatcct
     2761 cagtaatgca ggtggaccga aacgtgaaaa tgggatggtc cacttggcac gtgtcgaaga
     2821 aaatggtgag ttgacttggc tcaaacacaa tccaattcaa aaaggagagt ttgcctataa
     2881 ttcgctccaa gaattaggaa atggggagta tggcatcttg tatgaacata ctgaaaaagg
     2941 acaaaatgcc tataccctat catttagaaa atttaattgg gaatttttga gcaaagatct
     3001 gatttctcct accgaagcga aagtgaagcg aactagagag atgggcaaag gagagatggg
     3061 caaaggagag atgggcaaag gagttattgg cttggagttc gactcagaag tattggtcaa
     3121 caaggctcca acccttcaat tggcaaatgg taaaacagcg actttcctaa cccagtatga
     3181 tagcaagacc ttgttgtttg cagtagataa ggaagatatc ggacaggaaa ttattggtat
     3241 agctaaagga agcatcgaaa gtatgcataa tcttcctgta aatctagcag gtgccagagt
     3301 tcctggcgga gtaaatggta gcaaagcagc ggtgcatgaa gttccagaat ttacaggggg
     3361 agttaatggt acagagccag ctgttcatga aatcgcagag tataagggat ctgattcgct
     3421 tgtaactctt actacaaaag aagattatac ttacaaagct cctcttgctc agcaggcact
     3481 tcctgaaaca ggaaacaagg agagtgacct cctagcttca ctaggactaa cagctttctt
     3541 ccttggtctg tttacgctag ggaaaaagag agaacaataa gagaagaatt ctaaacattt
     3601 gattttgtaa aaatagaagg agatagcagg ttttcaagcc tgctatcttt ttttgatgac
     3661 attcaagctg atacgaaatc ataagaggtc tgaaactact ttcagagtag tctgttctat
     3721 aaaatatagt agtttgaaat aagatgtgaa caactctatc aggaaagtca aattaattta
     3781 tagaaaaatt ttagcagtca aggtgtactg ttatagattc aatatattat gcgactatta
     3841 accttgtctt ctcctaaaat tgacttt
//
LOCUS       DAVKHZ010000107         3859 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000107 DAVKHZ010000000
VERSION     DAVKHZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.107"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(163..1578)
                     /locus_tag="VWW99_001958"
     CDS             complement(163..1578)
                     /locus_tag="VWW99_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9539748.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1591..2802)
                     /locus_tag="VWW99_001959"
     CDS             complement(1591..2802)
                     /locus_tag="VWW99_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9539749.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGSNLAQGAASMAVALKSKNNNTKQIAFAAGFSALLAGITEPALYG
                     VTLKYKKPLYAAMIGGGLAGLFAGLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALI
                     VAVISVVITFVLAYIFGIDEEESSSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTF
                     SDKLIGDGVAIIPSEGKVYAPFDGKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELK
                     GQGFISHVEEGDRVFKNQLIFEMDLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQT
                     IEHSKELLVIL"
     gene            complement(2820..3659)
                     /locus_tag="VWW99_001960"
     CDS             complement(2820..3659)
                     /locus_tag="VWW99_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9539750.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
BASE COUNT         1259 a          753 c          481 g         1366 t
ORIGIN      
        1 ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatta atccccctat
       61 aaaataaaga ggtttagaat ttgatgtctt ccaaacctct ttatttcata tttaatgaga
      121 tgctgcctta accgtgataa tatctaatca tcaataaaca aactatttga ataaggattc
      181 tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat atcgattgaa
      241 tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtacctct tagaaagctg
      301 tgcagtggac atagaaacgc agtcaataca tccccaagac gtatagccca taatttccac
      361 accatcttgt agagcttcag ctacttgcaa taaatgttct ttcatatact gaattctata
      421 atcatcttgg actgttaagt tattaaattc atcttttatt agttgatctt tagcacctaa
      481 tccgttttcc actataaata atggaatttg ataacggtca taatatctat ttaaaattat
      541 acgtagtcca attggatcaa tttgccatcc ccactcagaa gactctaaat aaggatttac
      601 taaaccacca ataatattcc cttctcctga attatactgt gttggaagag cagattgagt
      661 cacactcatg taatagctaa aggataaaaa atctacagta taatttttta ataactctgc
      721 atcttcagct gcaaactcta tgttaatgtc attttcctta aaatatcttt ttgcataatt
      781 cggataataa cctctaacat gcacatctga aaatagataa tttagattct catactcatg
      841 agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag ctaataccat
      901 acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca aactcgaggc
      961 gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct ccttaggtat
     1021 atctattcct ccactagtaa acggtaattc caaaacagag tttacttcgt taaatgtaag
     1081 ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt tttcataaaa
     1141 atgaatcatt ctcctatcaa tccatccatg atattttctt gctaaatata atggagtctc
     1201 atagtgtgaa agagttacaa gtggttctat cccgtgagca tgtagttcat caaacaattc
     1261 atcataatat ttcaacccag cttcgttagg ttcttcctca tctccttttg gaaaaattct
     1321 actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca aggatatatc
     1381 ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt ctgtaggatt
     1441 ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata agcccttacc
     1501 atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc aaagaaaatc
     1561 atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt ccttagaatg
     1621 ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg tattcgttac
     1681 aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt ccatttcaaa
     1741 aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa aaccttgacc
     1801 ttttagctca acagtatcta atccaatatg aattagtaac tcaacaccct catcactctt
     1861 caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa atggtgcata
     1921 aaccttacct tcacttggaa taatcgctac tccgtctcca attagtttat ctgaaaatgt
     1981 tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa atatcatttt
     2041 tttatttgaa actccagctt taacttctaa attgctagaa ctctcttctt catcgattcc
     2101 aaatatataa gctaatacaa aggtaataac aaccgaaata accgccacaa ttaaagcatt
     2161 tacaatattt gatggcacat cagaataaat aaattgaggc aacgctatca aagatgggac
     2221 agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta atccaccacc
     2281 aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata atgcaggttc
     2341 ggtaatccct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt ttgtattatt
     2401 atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt ttgaccctaa
     2461 cattgctgga atgattggag tcatggaacc agcgatatac tcaatgattc tttctaaaat
     2521 attccctttg tgcccttgaa caactgaatc ggattcaaaa ttgccaagtt taacgaattc
     2581 tttataataa ttagctacat cattaccaag tataatttga tattgtccat tctttttcat
     2641 aatacctatt acacctggta tcttcttcac atcatcatca ttgactaaat tttcatcttt
     2701 taattctaat cttaaacgtg ttacacaatg ggtaactcta ttgacatttt tttcacctcc
     2761 aattacatcg aggatttttt gtaccgtatc tttataactc atggtattct cctattctat
     2821 taatctaaat tttttgttaa gcgacgaata tgagccatca aataaactaa ttcactagaa
     2881 gtcagcaaat aattgtactc cgtttgtata aacattgcca cctgttcacc acattcatat
     2941 tctctaggat atttattttt cattaatgct aacaagtctt catcatcatc gtcacaataa
     3001 gttgtatttt caaccaagcg atgggcaaat aattttatat gagttatgaa tctataataa
     3061 tcaatagaat cttggttaaa ctcagtacaa tagaaatctt gaatgatttt ctcaattccc
     3121 ataattattt cagtgatttt atacgactct tgaaaattat tttctaaatt agcattaaca
     3181 aaatgtagcg ctatgaatgc agcttcgtca attggaagtt caatacctaa ctcttttttt
     3241 attatttgta aagccctttt cccaagttca aattcttctt tataatattt cgatatttcc
     3301 cagcgtaggg gattcgaaat aataatccct tctttataac gttctatgct cgaagaaata
     3361 tgatccgtta aattaatata gataatttca tcaaaattat tacccaatgt tattttacct
     3421 aggttaatta ttttttcggt gatgtacacc aactccaaag gaacgttaat aaacatctct
     3481 gtaaaacggg tcatattatc tctatttttg agaaaaaaac ttttctcaat atcaattgga
     3541 tttactttat ctccagtttt tcttccaaaa gctattcccc ttccaaaaag aagaatatcg
     3601 atatcttttt tactttgcgc aattacggca ttgtggttta gtattcgttt tatgatcata
     3661 aaatttccct tttaaaataa aaggaaccta cttaaaaaca tcaagaaact tcttctgatg
     3721 tgattaaata ggttcctgct cacttgtatt agtgataaca tccttttatt tcaattttta
     3781 atggtgacat gaagcttaaa taattaataa ctctgtaacc gtttttattt tatgatattt
     3841 caatctattt gtcaagtac
//
LOCUS       DAVKHZ010000108         3802 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000108 DAVKHZ010000000
VERSION     DAVKHZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.108"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            154..810
                     /locus_tag="VWW99_001961"
     CDS             154..810
                     /locus_tag="VWW99_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9539751.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            918..2013
                     /gene="prfB"
                     /locus_tag="VWW99_001962"
     CDS             join(918..989,991..2013)
                     /gene="prfB"
                     /locus_tag="VWW99_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9539752.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQGEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            2031..2723
                     /gene="ftsE"
                     /locus_tag="VWW99_001963"
     CDS             2031..2723
                     /gene="ftsE"
                     /locus_tag="VWW99_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9539753.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            2716..3642
                     /locus_tag="VWW99_001964"
     CDS             2716..3642
                     /locus_tag="VWW99_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539754.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         1196 a          622 c          864 g         1120 t
ORIGIN      
        1 aaaatttctt accttcattc acagagctcg atttcagagc tctttttgct agcttattca
       61 tacttttctg aatttcgaaa aagaaatgta agcgtttgat agatttacaa aaagattgta
      121 taatagggat aagaatagaa aaggagaagt ctcatggcag ttaaagattt tatgacccgc
      181 aaggtagttt atattagtcc agatacaaca gtatctcatg cagcagattt gatgagagag
      241 caaggtttgc accgtctgcc tgttatcgaa aatgatcaat tagttggttt ggtgactgag
      301 ggaaccattg cacaagcaag tccatctaaa gcaacaagtc tttctatcta tgagatgaat
      361 tatcttctga ataagacaaa agtaaaagat gtcatgattc gcgatgttgt cactgtctca
      421 ggctatgcta gtctagaaga tgcaacttat ctgatgttga aaaataagat tagtattctc
      481 cctgtcgtag ataaccatca agtatacgga gttattactg accgtgacgt tttccaagcc
      541 tttcttgaaa ttgcaggtta tggcgaagaa gggattcgtg tacgctttgt tacagaagat
      601 gaagttggtg ttcttggaaa aattgtttct ttgattgtag aagaaaattt gaatatctcc
      661 catacagtca atattccgcg taaggatggt aaggtgatta tcgaagtgca aatcgatgga
      721 tcaattgatt taccagcctt gaaagaaaaa tttgaagcaa atggtattca agtggaagaa
      781 atcgctcgca cttcagcaaa agtcttgtaa gaagggaagc ccaaaggctt cttttttcat
      841 gaaaagggga ttagagaaaa agatggaaag aaatgataaa atatgctata atgaaataat
      901 gtaaaaaagg agtatttatg gacatttcag taattcgtca gaaaattgac gcaaatcgtg
      961 aaaaattagc ttctttcagg gggtctcttt gacctcgaag ggctagagga agagattgcc
     1021 atcttggaaa acaagatgac agaacctgat ttttggaacg ataatattgc ggcccaaaaa
     1081 acgtcgcaag aattgaatga attaaaaaac acttacaata ccttccataa gatggaagag
     1141 ttgcagggtg aagtcgaaat tttattggat tttttggctg aagacgagtc agtgcatgat
     1201 gaactggtag cgcagttagc cgaacttgat aagataatga ccagctacga gatgactcta
     1261 ctcttgtcag aaccttatga ccacaacaat gccatcttgg aaatccatcc aggttctggt
     1321 ggtactgagg cgcaggactg gggtgatatg ttgcttcgta tgtatactcg ttatggtaat
     1381 gctaaaggct ttaaagtgga agtgttggat taccaagcag gtgatgaggc tggtattaag
     1441 tcggtaactt tatcatttga agggcctaat gcctatggtc tcctcaagtc agaaatgggt
     1501 gttcaccgct tagtgcgaat ctcaccattt gactctgcca aacgtcgcca tacctctttc
     1561 acatctgtag aagtgatgcc agaattggat gatactattg aagtggaaat ccgtgaagat
     1621 gatatcaaga tggatacctt ccgttcaggt ggtgccggtg gacaaaacgt caataaggtt
     1681 tcaacaggtg tacgtttaac ccacattcca actggaattg ttgtccaatc aacagtggat
     1741 cgtacccagt atggaaatag agatcgtgcc atgaagatgt tgcaggctaa gctctatcaa
     1801 atggagcaag ataagaaggc tgcggaggta gattctctca aaggtgagaa aaaggagatc
     1861 acttggggaa gccaaatccg ttcttatgtc ttcacgcctt atactatggt aaaagatcac
     1921 cgaactagct ttgaggttgc tcaggtagat aaggttatgg atggggacct agatggtttt
     1981 atcgatgctt atctcaagtg gcgaattagc taagatagaa aggaactcac atgtcaatta
     2041 ttgaaatgag agatgtcgtt aaaaaatacg acaacggaac gactgctcta cgcggtgttt
     2101 cggttagcgt tcaaccgggg gaatttgctt acatcgtagg accttcagga gcagggaagt
     2161 caacttttat tcgttctctg tatcgtgaag taaaaatcga taaaggaagc ctatcagttg
     2221 ctggttttaa tctggttaag atcaaaaaga aagatgtccc gcttctacgt cgtagtgttg
     2281 gggttgtctt ccaggattat aaattgttac caaagaaaac tgtctatgaa aatattgctt
     2341 acgctatgga agtaatcggg gaaaatcgcc gtaatatcaa aagacgagtg atggaagttt
     2401 tggacttggt tggattgaag cataaggttc gttctttccc aaatgaactc tcaggtgggg
     2461 agcaacagcg gattgcgatt gcgcgtgcaa ttgtaaataa tcccaaagta ttgatagctg
     2521 atgagccaac aggaaatctg gatccggata attcatggga aattatgaat ctcttggaac
     2581 ggattaacct acaaggaaca actattttga tggcgactca taatagccag attgtaaata
     2641 ccttgcgcca ccgtgtcatt gccattgaaa atggccgtgt cgttcgtgac gaatcaaaag
     2701 gagagtatgg atacgatgat tagtagattt tttcgccatt tatttgaagc cttaaaaagt
     2761 ttgaaacgaa atggttggat gacagtagct gctgtcagtt cagtcatgat tactttgacc
     2821 ttggtggcaa tatttgcatc tgttattttc aatacagcga aactagctac agatattgaa
     2881 aataatgtcc gtgtagtagt ttatatccga aaggatgtgg aagataatag tcagacaatt
     2941 gaaaaagaag gtcaaactgt tacaaataat gactaccaca aggtatatga ttctttgaag
     3001 aacatgtcta cggttaaaag tgttaccttt tcaagtaaag aagaacaata tgaaaaatta
     3061 accgagataa tgggagataa ctggaaaatc tttgaaggag atgccaatcc tctctatgat
     3121 gcctatattg tagaggcaaa cactccaaat gatgtaaaaa ctatagccga agatgctaaa
     3181 aaaattgaag gtgtctctga ggttcaagat ggcggtgcca atacagaaag actcttcaag
     3241 ttagcttcat ttatccgtgt ttggggacta gggattgctg ctttgttaat ttttatcgca
     3301 gttttcttga tttcaaatac cattcgtatt accattattt cccgcagtcg cgaaattcaa
     3361 atcatgcgct tggtcggagc taaaaacagt tatatccgtg gaccgttctt gttagaagga
     3421 gcctttatcg gtttattggg agctatcgca ccatctgttt tggtctttat tgtttatcaa
     3481 attgtttacc aatctgtcaa caaatcgttg gtagggcaaa atctatccat gattagtcca
     3541 gatttattta gtccgttgat gattgcccta ctatttgtga ttggggtttt cattggttca
     3601 ttgggatcag gaatatccat gcgccgattc ttgaagattt aggtaaaata gctgctttta
     3661 tgaggagatt gtaaaatctc cttttttgct acaagagttt ttgaaaagag atgcgtagaa
     3721 gaaaagagct tccaaagaag tcccccagag aagacttctt gatggctaat ttgaacttga
     3781 aatttggcgc actagaaatt tt
//
LOCUS       DAVKHZ010000109         3770 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000109 DAVKHZ010000000
VERSION     DAVKHZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.109"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..287
                     /locus_tag="VWW99_001965"
     CDS             <1..287
                     /locus_tag="VWW99_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539755.1"
                     /translation="YGTVDNHVLTLIFLIKICVDTTIKFLFIRLFDTSTLMKRRIFFL
                     FALDVIVYLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEFELFKNSK"
     gene            1421..1660
                     /locus_tag="VWW99_001966"
     CDS             1421..1660
                     /locus_tag="VWW99_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539756.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1645..1923
                     /locus_tag="VWW99_001967"
     CDS             1645..1923
                     /locus_tag="VWW99_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539757.1"
                     /translation="MYNDVIERISLCEFIGDIFYSKITSCCIVAKDVSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSEVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            2209..>3770
                     /locus_tag="VWW99_001968"
     CDS             2209..>3770
                     /locus_tag="VWW99_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEU9539758.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTFVRAEESPVASQSKAEK
                     DYDAAVKKSEAAKKHYEEAKKKAKEAQKKYDEDQKKTEDKAEAVKKADEELQKANLEV
                     QKAYVEYREAKAKDKASAEQKIKEATQKQEEANKKFNEEQAKVVPEASDLAVTKQKAE
                     EAKKEAEVAKKKSEEAAKEVEVEKNKILEQDAENEKKIDVLQNKVADLEKEIADAEKT
                     VADLEKEVAKLEKDVEGFKESDGEYAEFYLEAAEKDLATKKAELAEAKIKATTKKAEL
                     EKAEAELENLLSTLDPEGKTQDELDKETAEAELNKKVEALQNKVAELEEELSKLEDNL
                     KVAETNNVEDYIKEGLEEAIATKQAELEKTQKALDTALNELGPDGDEEETPAPAPKPE
                     KPAPAPAPKPEQPAPAPKPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPAPA
                     PKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQYN
                     GSWYYLNANGAMA"
BASE COUNT         1411 a          551 c          735 g         1073 t
ORIGIN      
        1 tttatggtac agttgataat catgtactga ctcttatctt tttgataaaa atttgtgtag
       61 atactacgat aaaattttta ttcatcagat tatttgacac aagtacttta atgaagagga
      121 gaatattttt tcttttcgca ttggatgtta tagtttactt atttttagga tatcttttag
      181 tgattcaaaa agcaggctat ttgttttcgg ttttacttct tttttctaat ttttcagttc
      241 catttatcaa agaaaaggaa ttcgaattat ttaaaaatag caagtaatag aaacataggt
      301 taaatcttag aacggcttaa aatcagatat gataaaggga aatagtctta tctaggtaac
      361 taagtgctac aaacacagtc gaggatcact tgttaggatt acattttaat catgcttaat
      421 aagaatggag aattcctatg gggattgtat tactctgaag tttcgaatac cttagttctc
      481 gtgattttga gctattctag ctttagaatc cttcagaatg taaaatttta agcagtcaaa
      541 gacttagaat agtccgaaaa catttatttt atagcaattc taagattaga attgctcata
      601 tatatgtatt tgagctgctc gatagttagg acagctctat tctgagaaat ctgagctatc
      661 aatgcttcag aatagtattt cgccaatggt ttttattgaa gaggtgattg tgttatgata
      721 gtctaaaata aaactgattt aatggagtaa atggagaatc gttacatgaa acattttttt
      781 gcaggaattg gtgagataaa acttgttggc tacttattgt atatatgtgt aggactagcc
      841 cttctttttc atatatatgt cataggttcg gaactgagtt ttgtgaaaat agtgttatct
      901 attttgggag ttatatttgt aagtatgtta actattgctc gaatttatcg gacttttcta
      961 taaagaatag tttggacttg aactgtatct aaaaacagag gttggtcgat tatcagcctc
     1021 tttcttgtcg ttgaaaagag tgagatatat tgacttttga aaaaaagatg tcataattga
     1081 gttgggataa acctaaaagt aatatagtag attgaatctg gaatagtccg ttatgactgt
     1141 taaaatattt ctagaaatta atttgacttt cctaatcgat ttgttcacat cttgtttcaa
     1201 tctactatac tttctcgtta aaccgttcgg caattcatgg tgacatggac acatagtagt
     1261 aagcacacta tgtggcttgg cagagctaaa aactgttccc ttgcgataag cctaataagc
     1321 acaacatagg gagttagaga agccgactct aatcaaccac tttgggcagt agtgagaact
     1381 gccccgtgct ttttattttg agaaaatatg gagtttgtcg ttgaaattac ttgattgtat
     1441 tttggactat caagaaagat tcaatggaaa aacatgtcaa gtatcaacga attataagta
     1501 tttagagatt ttcaaagtga atttttgctt gactgattta catcatttat ttggcttaca
     1561 caaaatcaca cgagattatg ctagtcaaac aaaacctgct attcaagctg gtgtttttat
     1621 tttagaagat tttagaaata tcctatgtac aatgatgtaa tcgaaaggat atctttatgt
     1681 gagtttatcg gcgatatttt ctattctaaa ataacaagtt gttgcatcgt agctaaggat
     1741 gtatctaaaa atactatgaa attggacgtc atattttttg aggataaaaa taaaagatcc
     1801 gcagttttag gtttacgaag agacaaaagc gaagtattta aaccagttac tctacatttt
     1861 acaagcgcta agaaatatgc taaagttcgt aaaacagatg tgaaagaaat gaaatggcta
     1921 taaagattgt ccgcaggctt aagcttgcgt tttttgttgt ttaacgaaat taaaaaacaa
     1981 gctctctcat cggaaatgtt tttataggaa tgaaggaaga tgatgctatg tttaaaaaga
     2041 gaatcgaaaa gggcaaaagt agtgtcttta tctttttgga gtagaagctt atgatataga
     2101 aatttgtaat aaaaatgtaa tataaaacat ttgacaaata tttacggggg gagtatactt
     2161 aatataagta tagtctaaaa atgactatca gaaaagaggt aaatttagat gaataagaaa
     2221 aaaatgattt taacaagtct agccagcgtc gctatcttag gggctggttt tgttgcgtct
     2281 cagcctactt ttgtaagagc agaagaatct cccgtagcta gtcagtctaa agctgagaaa
     2341 gactatgatg cagcagtgaa aaaatctgaa gctgctaaga aacattacga agaagctaaa
     2401 aagaaagcaa aagaagctca gaaaaaatat gatgaggatc agaagaaaac tgaagataag
     2461 gcagaggcag ttaagaaagc tgatgaagaa cttcaaaaag caaatttaga agttcaaaaa
     2521 gcttatgtag aatatagaga agcgaaagcg aaagataaag ctagcgctga acagaagatt
     2581 aaagaagcaa cacaaaaaca agaagaagcg aacaaaaaat ttaatgagga gcaagcaaaa
     2641 gtagttcctg aagcaagtga cttagctgtt actaaacaaa aagcggaaga agctaaaaaa
     2701 gaagcagaag tagctaagaa aaaatctgaa gaggcagcta aagaggtaga agtagagaaa
     2761 aataaaatac ttgaacaaga tgctgaaaac gaaaagaaaa ttgacgtact tcaaaacaaa
     2821 gtcgctgatt tagaaaaaga aatagctgat gctgaaaaaa cggtagcaga tttagaaaaa
     2881 gaagttgcta aacttgaaaa agatgtagaa ggtttcaaag aatcagatgg tgaatatgcc
     2941 gaattctacc ttgaagctgc tgaaaaagac ctagctacta aaaaagctga attagcagaa
     3001 gctaaaatta aagctactac taaaaaagct gaattagaaa aagcagaagc tgaacttgaa
     3061 aacctattat ctacactaga ccctgaaggt aagactcagg atgaattaga taaagaaact
     3121 gctgaagctg agttgaataa aaaagttgaa gctcttcaaa acaaagttgc tgaattagaa
     3181 gaagaacttt caaaacttga agataatctt aaagttgctg aaacaaacaa cgttgaagac
     3241 tacattaaag aaggtttaga agaagctatc gcgactaaac aagctgagtt agaaaaaact
     3301 caaaaagcat tagatactgc tcttaatgaa ttaggtcctg acggtgatga agaagaaact
     3361 ccagctccag caccaaaacc agaaaaacca gctccagctc ctgcaccaaa accagagcaa
     3421 ccagctccag caccaaaacc agagaagtca gcagatcaac aagctgaaga agactatgct
     3481 cgtagatcag aagaagaata taaccgcttg actcaacagc aaccgccaaa agcagaaaaa
     3541 ccagctccag ctcctgcacc aaaaccagag caaccagctc ccgcaccaaa aacgggctgg
     3601 aaacaagaaa acggtatgtg gtacttctac aatactgatg gttcaatggc gacaggatgg
     3661 ctccaaaaca acggttcatg gtactacctc aacagcaatg gcgctatggc tacaggatgg
     3721 ctccaataca acggttcatg gtattaccta aacgctaacg gcgctatggc
//
LOCUS       DAVKHZ010000110         3674 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000110 DAVKHZ010000000
VERSION     DAVKHZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.110"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            108..1517
                     /locus_tag="VWW99_001969"
     CDS             108..1517
                     /locus_tag="VWW99_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9539759.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1535..2830
                     /locus_tag="VWW99_001970"
     CDS             1535..2830
                     /locus_tag="VWW99_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9539760.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2835..3143
                     /locus_tag="VWW99_001971"
     CDS             2835..3143
                     /locus_tag="VWW99_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9539761.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3140..3448
                     /locus_tag="VWW99_001972"
     CDS             3140..3448
                     /locus_tag="VWW99_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9539762.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1242 a          617 c          707 g         1108 t
ORIGIN      
        1 gaaatgaggc aaacacaaga tatgtatcaa caatttttaa aaatgtctag acaaatttta
       61 aaattatgct atactttatg tatagaataa tagaaaggag gttgagcatg aaaaaaaagt
      121 tagtatttcc taatctgttt tggtggggag cagcttctag cggacctcag acagaaggtc
      181 aatatggaaa agtacatgaa aatgtgatgg actactggtt caaaacgcat ccagaagatt
      241 ttttcgataa tgtcggacct cttgtagcca gtaacttttt tcatacttac accgaagatt
      301 tccacttgat gaaggaaatt ggagttaatt ctttccgcac ttccatccaa tggagtcgac
      361 tcatcaagaa tttagagaca ggtgagcctg atccaaaagg tattgctttc tacaatgcca
      421 tcattgaaga agctaaaaag aaccagatgg atcttgtgat gaatttacat cattttgatt
      481 taccagtgga acttcttcaa aaatacggtg gttgggaaag caaacatgta gtggagttat
      541 tcgtgaagtt tgccaagact gctttcacat gctttggaga taaggttcat tactggacaa
      601 ctttcaatga gccaatggtc attccagaag caggatactt atatgctttc cattatccaa
      661 atctaaaagg aaagggaaaa gaggccgtac aagtcatcta taatctaaac cttgctagtg
      721 caaaagtgat tcaactatat cgctcattag aacttgatgg aaagattggg attattttaa
      781 acttgacacc tgcttatcca agaagtaatt ctccagaaga cttagaagca agtcgattta
      841 cagatgactt ctttaacaaa gtcttcttga atccagctgt taaaggaact ttcccagaaa
      901 gattggtaaa acagctagag agagatggcg tgttatggag tcataccgaa aaagagcttc
      961 aactgatgaa atcaaatacg gttgattttc ttggagtaaa ctactaccat ccaaaacgtg
     1021 ttcaagcaca agcaaatcct gaggaatatc agacgccctg gatgccagac caatacttca
     1081 aagagtatga atggccggag cgtcgcatga atccatatcg tggttgggaa atttttccga
     1141 aatccattta tgatattgct atgattgtga aggaagaata tggtaatatc ccatggttta
     1201 tcagtgaaaa cggaatgggt gttgaaaacg aagcacggtt tatcgatgaa aatggagtta
     1261 tcgatgacgt gtatcgtatt gaattttatg aagaacattt aagatggcta cataaagcca
     1321 ttgaagaggg aagtcactgt tttggatacc acgcttggac cgcatttgat tgctggtctt
     1381 ggaataatgc atataagaat cgttacggat ttatctccgt tgatttagaa acgcaaaaga
     1441 gaaccatcaa gagctcagga agatggtatc gcaaagtaag tgacaataac ggttttgaag
     1501 tagaaattga ggagtaaaga gaggagatat tcatatgaaa aactcaaaat ttatagacca
     1561 atttgccacc tttgctggta aactagggaa ccaaattcat ttaaaaaccc taagagatgc
     1621 attcgtaaca gtaatgccat tatatatttt ggcaggttta atcgttcttt tgaacaacac
     1681 ggtatttaag tggattttcc aaggggatac attaacaaga ttccaatatt ggggaataac
     1741 aattgcaaac ggtactttaa gtatttcagg tatgattatt gctgtaatgg ttggttattt
     1801 cttagctaaa aacagagatt tcgaaaaccc gttagcagca tcaatgctat cattagtttc
     1861 tttaattgtg atgatgccaa atacagtttc tgtagttcct gacggagcaa aagatgctgt
     1921 aaacatttca ggtgttcttt cattcaacaa cacaggtaca ggcgcaatgt tcgccggggt
     1981 tatcgtagcg attattgcaa cagaattatt cattgaatta tcaaatgtta aagctttaca
     2041 aatgaacctt ggtgaaaata ttccaccagc tgttagtaga tcatttagcg tattacttcc
     2101 agtcatgacc gtcatctcct tatttggggt tgtttcagca ttattattca atataactgg
     2161 aatgaactta atctcaatca ttacaatctt tattcaagaa ccaattcgtc atattggtac
     2221 aagcttaatc ggggtcatta ttatttactc tttaggaaat atgttatggc tatttggtat
     2281 tcaccaagca gttatttaca gtgccatcct agaaccatta ctattaatta acattactga
     2341 aaacatcact gcagcaaata atggacaagc cattccacac atcatcaacc tatcacaaat
     2401 acaaacattc gctttaatgg gtggtagtgg atctacatta tgtttattaa tagcaacatt
     2461 cttagtgagt cgcaatgctg tctctaaaaa cgtggctaaa ttatcttttg gacctggtat
     2521 cttcaatatc aatgaaccag tattattcgg ttacccaatc gtttataata tttcattagc
     2581 tattccattt atcacagttc cagtccttgg tattttaatc agctacttag caacagttac
     2641 aggattcatg agtcctgcat ttatacaagt tccttggact acaccagtat tcttaaatgc
     2701 atggttagca acagcagggg acgtgagagc agttctagtt caattcatca tctttgcact
     2761 tggagttctt ctatacattc catttatcaa agttaatgac aaagttgttg aacaagaaat
     2821 ggaaggttaa gattatgaaa caaattttat tagtatgtaa tgcgggaatg tcgacaagta
     2881 tgcttgttaa aaaaatgcaa caaagtgcga ctgaacgtgg gattgaaatc tctatccaag
     2941 caaaatcaat gacagaagct aaaaagaata tccatgaagc agatgtcatt ctaattgggc
     3001 cacaaattcg ttatgagtta cttgctgtga aagaaattgc aggaaatatc cctgtagata
     3061 ccattgatat gcgggattac ggaatgatga atggagcaaa agttctggaa caagcacttg
     3121 catggatagg agaaataaga tgacagaaga aatggaatat atctgttttc agctcattgc
     3181 caatagtgga gcagccaaat catcctttat tgaagccatc caactagcta aggcaggaaa
     3241 tttaaaagaa gcgaaaatta aagttgaaga agcggaagac tccttagtag aagcgcacaa
     3301 gattcactct aatctcatac aaaaagaagc tactggagaa aaaataggat tctccttgct
     3361 atttatgcat gcagaagacc aaatggcttc aacagagatt attcagttgc tatctaaaga
     3421 attcatcgat ctctatcaaa ataaatagga acactgtcat tcccatctgt ctttattaat
     3481 gacgactaca ttgtaggaac ctccaaaagt tggacaaaat gattgaaagg attggatcct
     3541 gcattacaca agatccaatc cttttctttt actctagttt ttataatatg ttgaatctag
     3601 aatagtccac tgtagtttct aaaaaattgc tggaaatgga tgttaagctc caatttattt
     3661 gtttatatct tatt
//
LOCUS       DAVKHZ010000111         3632 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000111 DAVKHZ010000000
VERSION     DAVKHZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.111"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(144..1280)
                     /gene="lctO"
                     /locus_tag="VWW99_001973"
     CDS             complement(144..1280)
                     /gene="lctO"
                     /locus_tag="VWW99_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9539763.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKDEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1904..3394)
                     /gene="lysS"
                     /locus_tag="VWW99_001974"
     CDS             complement(1904..3394)
                     /gene="lysS"
                     /locus_tag="VWW99_001974"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9539764.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3522..>3632)
                     /locus_tag="VWW99_001975"
     CDS             complement(3522..>3632)
                     /locus_tag="VWW99_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9539765.1"
                     /translation="DFINTYDLETMAQQVIPKAAFGYIASGAEDTFASFQ"
BASE COUNT         1043 a          779 c          708 g         1102 t
ORIGIN      
        1 tctaaaacat tgttagaaat cgatttaaat atcctgattg atttgtcctg tttttattcc
       61 attttactat ggttttcgat agcgatttat tcttccaata cacgaagaaa aacctccaca
      121 ttcagtggag gcaatctgtt ttatcaatac aattttaagt cacgagggtc aactgggaag
      181 gttgggttgt atggattgtg acggagctta aagtgtttga catcttcaat ggtctgagct
      241 ccagacaatt gcataactgt cttcaattcc gcattcaagt gttcaaagac ttgacgcaca
      301 ccgacactac caccgagagc caagccataa ataaccgggc gaccaatagc taccaagtct
      361 gctcctgaag ccaaggcttt aaagacgtgt tgaccacgac gaacaccaga gtcaaagaca
      421 atcggcacac gtctatcaac tgcttctgcc acttcttgaa gcgagtcaaa ggcagctggt
      481 ccaccgtcga tttgacgacc accgtggttg gttacccaga taccagaagc tcctgcagca
      541 agcgaacgtt caacgtcctc acggcattgt ggtcccttga catacacagg aagaccagag
      601 tattcagcga taaattctac atcgcgtgga gacaagcgtt gtttagctga tttgtaaaca
      661 aagtccattg atttaccagc accttctggc aggtattctt caacaatcgg catgccaact
      721 gggaagacaa aaccattacg cttatccact tcacgattcc cccctacagt agcatctgcc
      781 gtcaagacaa tcgctttata accttcatcc ttcacacggt ccatgatgtg gcggttgata
      841 ccgtcatcct tactaaagta aaattgaaac caatgaggtg tcccttgaag ggcttcagaa
      901 atctctggaa ggtcaacagt agagtaagaa ctggttgtat aaagagaacc aaactcatgc
      961 acaccacgcg cagtcgccac ttccccctgt tcatttgcca atttatgagc cgcaacaggt
     1021 gccataatga ttggcgaaga tagtttttca cctgcaaatt caatctctgt acttggattt
     1081 tcaacatcgc aaagtgtatg aggaacgatg agtttgtggt taaaggcgcg gatattctct
     1141 cttaaagtga aagtatcttc cgccccacta gcgatatagc caaatgctgc tttaggaata
     1201 acttgttgcg ccattggctc caaatcatag gtattgatga agtctacatg accttctgca
     1261 ttgcttgttt tgtatgacat aaactgtcct cctcgattaa gtaagcgttt gctttttact
     1321 atacaactat aataactctt ttttcaatca ttttcaagag ttttgcttaa aaaccatctt
     1381 attttttcat acaatatact agccctactt tccttaaact ctaaacctta gtctatatta
     1441 agtgaatcgt tgagattttt tgctgccagg catttgaact acttttttat ttgctgattc
     1501 tatctactaa tactataaaa aacaagtgaa taagacacca gaacatcctc taataggacc
     1561 tatggactta tatcagtccc atatatactg tttaagcaag atactatcct agttgacgct
     1621 aacttcctat cccacttatt aattttttat ttatagtgaa tctgaacaaa tcaattgggg
     1681 aatccaagtc aatttctagt actttttgaa aagtttcagc gtactatcgt aaaatgaaat
     1741 aaaacatgca caaattgatt agggaattta aaccaatttc taacaatgtc ttagaaatca
     1801 aagtgtacta tttcaatttc aatacactac acttcatttt ctccttaaat tcagtctctt
     1861 tttgaataaa aaaatcctct ggtaagagcc agaggataag aatttatttc attgttggga
     1921 agagcaatac atcacggata gttgttgtat cagtgaggag catgcagaga cggtcgatac
     1981 cgattcccaa accacctgtt ggtggcatac cgtattcaag agcttcaatg tagtcatagt
     2041 cgattcctgt cgcttcatca tcaccaagtt ctttggcttt agcttgggct tcaaaacggc
     2101 taagttggtc gattgggtcg ttgagctcag taaaggcatt accgtactcc ttagtcatga
     2161 taaagagctc gaaacggtca gtaaagcgtt ggtcttcagg atttttctta gcgagtggag
     2221 atacagctac tggatgtcca tagacaaagg ttggttggat taaagtttct tcaacaaact
     2281 cttcaaagaa ggcattgatg atgtgaccaa cctcagtgta gtgtttctca actggaactt
     2341 tcttctcagc agcgatagct ttagcttctt ccaaagtcat gtcttgccag aaatcgacac
     2401 cagtaatttc tctgatagca tccaccatat gaacacgctt aaatggttcg ttaatcttga
     2461 tttcagtacc ttgatagttg actgggccat cgcccttaac tgatttagca gcatgttgga
     2521 taatgccttc cgtcaagtcc atgatatctt ggaagtctgc ataagcttgg taaacttcga
     2581 tagaagtgaa ctcagggtta tgagtagcat ccattccttc gttacggaag atacgcccaa
     2641 tttcatagac acgttccata ccacccacga taaggcgttt taagtgaagc tcagtcgcga
     2701 tacgaagcac catgtcaatg ttttgggcat tgtggtgggt gataaatgga cgggcagcag
     2761 caccaccggc ttcgttgtga agaacaggtg tttccacttc aaggaatcct ttttggtcaa
     2821 ggtaacgacg gatttcagag atgatttttg aacgagtgac aaaacgttca aagctttcac
     2881 ggttagaaat caagtcaagg taacgtttac ggtaaattgt ttcaacgtct gtcaaaccat
     2941 ggaatttctc aggaagagga cgaagagcct tagacaagtg tgtgatgtgg gttgccttga
     3001 tagagagttc tcccatatcc gtacgcatca cttcaccttc gacaccaagg aagtcaccaa
     3061 ggtctgcttt tttgaagatt tcgtagtttt cttcaccgac agcatcctta cgaacataga
     3121 tctgaatctg gccttcgcgg tcttgaaggt gggcaaaccc cactttacct ttaccacgtt
     3181 tggttaccaa gcgtcctgcg atagtagctg tttcgttttt atcgtgtaat tgttctttat
     3241 cgaggttggc atatttatct tttaattctt gtgaatttgc agtacgttca aaacgttttc
     3301 cgaaaggatc gattccttgt tcgcggagcg cagccatttt ttcacggcga acgatctgct
     3361 ggtcatttag ttcttccata tgttctgtag acatgggatc ctcctagttt tactcaaaat
     3421 tgaatacgat gagtcatttt ttgcgatact cccattttat cataaataag taagaaattc
     3481 acggataatc tttaaaaact aaaaagaacc tgacgctaaa atcactggaa agaagcgaaa
     3541 gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc
     3601 attgtctcca aatcataggt attgatgaaa tc
//
LOCUS       DAVKHZ010000112         3534 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000112 DAVKHZ010000000
VERSION     DAVKHZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.112"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            68..1417
                     /locus_tag="VWW99_001976"
     CDS             68..1417
                     /locus_tag="VWW99_001976"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9539766.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            1541..3001
                     /locus_tag="VWW99_001977"
     CDS             1541..3001
                     /locus_tag="VWW99_001977"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9539767.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            3015..3446
                     /locus_tag="VWW99_001978"
     CDS             3015..3446
                     /locus_tag="VWW99_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9539768.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT         1188 a          676 c          740 g          930 t
ORIGIN      
        1 acaaaaaaac ttgaaaaaaa cgaaggtaag cgttatgata gaaaagaaga aatattggag
       61 gaagaacatg tcacatatta aatttgatta ttcaaaagtt ttagacaaat ttgttgcacc
      121 acatgaagtg gaatacatgc aatcacaagt aacagcagca gatgaattga tccgtaaagg
      181 aactggtgct ggtagcgact tcttgggatg gttggacctt cctgaaaaat acgaccgcga
      241 agaatttgac cgcatcttga aagctgctga gcaaatcaag tcagacagcg atgttttggt
      301 tgtgatcggt attggtggat cttaccttgg tgcaaaagca gcaatcgact tcttgaatca
      361 ccattttgct aacttgcaaa caaaagaaga acgcaaagct ccacaaatcc tttacgcagg
      421 aaactcaatc tcatctactt accttgctga cttggtagag tatgttgcag acaaagactt
      481 ctcagtaaac gtaatttcta aatcaggtac aacaactgaa ccagcgattg ctttccgtgt
      541 ctttaaagaa ctcttggtta agaaatacgg tcaagaagaa gctaacaaac gtatctatgc
      601 aacaactgac cgccaaaagg gtgctgttaa ggttgaagca gacgctaacg gttggggaac
      661 atttgttgtt ccagatgata tcggtggacg cttctcagta ttgacagccg ttggtttgct
      721 tccaatcgca gcatcaggag ctgacatcaa agctcttatg gaaggtgcga atgcagctcg
      781 caaagactac acttcagaca aaatctctga aaacgaagct taccaatacg cagtcgttcg
      841 taacatcctt tatcgtaaag gctatgcaac tgagatcttg gtaaactatg agccatcact
      901 tcaatacttc tcagaatggt ggaaacaatt ggctggtgaa tcagaaggaa aagaccaaaa
      961 aggtatctac ccaacttcag ccaacttctc aactgacttg cactcacttg gtcaatttat
     1021 ccaagaagga actcgtatca tgtttgaaac agttgtccgt gttgacaaac ctcgtaaaaa
     1081 cgtgcttatt cctactttgg aagaagacct tgacggactt ggttaccttc aaggaaaaga
     1141 cgttgacttt gtaaacaaaa aagcaactga cggtgttctt cttgcccaca cagatggtga
     1201 tgtaccaaac atgtatgtga ctcttccaga gcaagacgct ttcactcttg gttacactat
     1261 ctacttcttc gaattggcaa ttgccctttc aggttacttg aatgctatca acccatttga
     1321 ccaaccaggt gttgaagctt ataaacgtaa catgtttgcc cttcttggaa aaccaggatt
     1381 tgaagaattg agcaaagaac ttaacgcacg tctataatag aagaaaagag tggtttgccc
     1441 actcttttta ctctctttat ccatagaaat tggactcagc caagacttgt gatataatat
     1501 agaaagcaaa aaggcagacg cctagataat aggagaaact atgtcaaaag atatccgcgt
     1561 acgttacgca ccaagtccaa caggactact acacatcgga aatgcccgta cagcattgtt
     1621 taactacttg tatgcgcgcc atcatggtgg aacatttctc atccgtatcg aagatactga
     1681 ccgtaaacgc catgtcgagg atggtgaacg ttcacaactt gaaaaccttc gctggttagg
     1741 catggattgg gatgaaagtc cagaatcaca tgagaattat cgccagtctg agcgtttgga
     1801 cttgtatcaa aaatacattg accaactatt agctgaagga aaagcctata aatcttacgt
     1861 tacagaagaa gagttggcag ctgaacgtga acgtcaagaa gtagctggcg aaacaccacg
     1921 ctacatcaat gaataccttg gtatgagtga agaagaaaaa gcggcttata tcgcagaacg
     1981 tgaagcagct ggtatcatac caacagttcg tttggctgtc aatgagtcag gtatctacaa
     2041 gtggcatgat atggtcaaag gtgatatcga atttgaaggt ggcaatatcg gcggtgactg
     2101 ggttatccaa aagaaagacg gttacccaac ttacaacttt gccgttgtta tcgatgacca
     2161 cgatatgcaa atctctcatg ttatccgtgg agatgaccat attgctaata caccaaaaca
     2221 gcttatggtc tatgaagctc ttggttggga agctccagag tttggtcaca tgaccttgat
     2281 tatcaactct gagactggta agaagttatc taaacgtgat actaatacac ttcaatttat
     2341 cgaagattac cgaaagaaag gttaccttcc agaagcagtc tttaacttta ttgctcttct
     2401 tggttggaac ccaggtggtg aagatgagat tttctctcgt gaagaactca ttaaactttt
     2461 tgatgaaaac cgcctcagca agtcaccagc agcctttgat cagaaaaaat tagactggat
     2521 gagcaatgat tatatcaaga atgcagacct tgaaaccatc tttgaaatgg caaaaccatt
     2581 cttagaggaa gcaggccgtt tgactgacaa ggctgaaaaa ttagttgagc tctataaacc
     2641 acaaatgaaa tcagtagatg agattatccc attgacagat cttttcttct cagatttccc
     2701 agaattgaca gaagcagagc gcgaagtcat gacgggtgaa acagttccaa cagttcttga
     2761 agcattcaaa gcaaaacttg aagcgatgac agatgatgaa tttgtgacag aaaatatctt
     2821 cccacaaatt aaagcagtcc aaaaagaaac aggtattaaa gggaaaaatc ttttcatgcc
     2881 tattcgtatc gcagtttcag gcgaaatgca tgggccagaa ttaccagata caattttctt
     2941 gcttggacgt gaaaaatcaa ttcagcatat cgaaaacatt ctaaaagaaa tctctaaata
     3001 aagaggatac aataatggac atctgggaaa agatgtacga agaagcacag aaactataca
     3061 atccacatga agtatcagac tttgtttatg ccaatcatgt tgtagctgca gtagaagcag
     3121 gagatggaca aatatttaca ggattctgta tggagggaac ctgtggtgtt ttccatctct
     3181 gcgcagaacg ggcgaccctc ttcaatatgt accaattttc aggacaaact aaggttaaga
     3241 aagtattagc ctttcgagac aaaccacctt atggtggaag ttcagccatg ccttgcggtg
     3301 cttgtagaga attcctttta gagcttaatg ctgaaaataa agatgcagaa ttcatgatgg
     3361 actataatat aagaaaaaca gttaaagtcg cagaactaat cccttattgg tggggagaag
     3421 aacgtgcttc taagtttaat gagtgataga agagtcagta taattctggc tctttttact
     3481 ataagttgaa aaaataaaaa aaattagaaa aagtagttga caaaatttga aaag
//
LOCUS       DAVKHZ010000113         3402 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000113 DAVKHZ010000000
VERSION     DAVKHZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.113"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..114)
                     /locus_tag="VWW99_001979"
     CDS             complement(<1..114)
                     /locus_tag="VWW99_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9539769.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGW"
     gene            complement(299..910)
                     /gene="rpsD"
                     /locus_tag="VWW99_001980"
     CDS             complement(299..910)
                     /gene="rpsD"
                     /locus_tag="VWW99_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9539770.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1105..2157)
                     /locus_tag="VWW99_001981"
     CDS             complement(1105..2157)
                     /locus_tag="VWW99_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9539771.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2154..2852)
                     /locus_tag="VWW99_001982"
     CDS             complement(2154..2852)
                     /locus_tag="VWW99_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9539772.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(3019..>3402)
                     /locus_tag="VWW99_001983"
     CDS             complement(3019..>3402)
                     /locus_tag="VWW99_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9539773.1"
                     /translation="GKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMN
                     HNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMA
                     SIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          952 a          762 c          651 g         1037 t
ORIGIN      
        1 ccagccataa atggtattac gtgagatttg gaaaacgtgt gatgcttctg ttatactacc
       61 tgttcgctca caataagaga gaactttttt acgaaaatct attgaatatg ccataaaaga
      121 ttataccaca ttgtgtacta tttttggttc attttactat atcactttgg actatatcgg
      181 tctagttatg tctcaacgat tatagctgga tactaagaga aatcaaaaaa gcactaaggg
      241 agaacgtccc aaagtgctta atatcaaggc ctcaaagcct gtcttattca ataaaatatt
      301 acaacatctt gttgtagaat tcaacgacaa gtgcttcgtt gatttctggg ttgatttcgt
      361 cgcgttctgg caagcgagtc aatgaacctt ccaacttttc agcgtcgaat gatacgaatg
      421 ctggacgtcc aagagtagct tctactgctt caaggattgc tggaactttc aatgattttt
      481 cacgaactga gatcacttga cctggagtta cgcggtatga tgggatatca acgcgtttcc
      541 cgtcaacaag gatgtgaccg tggtttacga attgacgagc ttgacgacga gtagtcgcga
      601 gaccaagacg gtaaacaacg ttatccaaac gacgctccaa aagaagcata aagttgaaac
      661 ctaggattcc gcctttgatt tttgtagctt gtacgaacaa gttacggaat tgtttttcac
      721 ctacaccgta agtgaaacga agtttttgtt tttcagccaa ttgcaaaccg tattctgaca
      781 atttagaacg gttgtttggg ccgtgttgtc ctggtacgta gttacgacgt gccaattctt
      841 tacctgtacc tgtaagtgaa aggccaaggc gacgagcttg tttccaagat ggtcctgtat
      901 aacgtgacat atgaatgtcc tcctgatata aataatattt cggtggaaat agtcacttag
      961 aaagccctga ttcgtgcaga tgcccttcgc ctaaacagcc aaggttactt gtcataagac
     1021 acctgttgac gagcttcatg ctttcctgct gatatttcac acaaaatcca ttttaccatg
     1081 aatagctgga tttgtaaagg ggttttaggc tttattttca ctaccagaga ggttgagaac
     1141 gagagtaaag gtacttccta gaccgtactg gctgctgact gtgatttccc cacccaattg
     1201 atgggccaat tcacgcgcaa tcgcaagtcc taatccatga ccacctgtct tcatgttacg
     1261 cgaagtttcg acacgataaa ggcgtttgaa aatattttcc aaatcctctg gggcaatacc
     1321 ctgcccttca tcggtcacac tgattgaaag ctggtccttc tccagcttag ccaccacttc
     1381 cagcttggtt cctggagcag aatatttaaa agcgttatcg accagattca ccaagatacg
     1441 agaaagctta gcataatctc cctcaatccg ggcagactct gggattacct gcaagtggac
     1501 atctcttctc tcctgctcaa tcaaaaactg aaattcactc atgcactcaa ttaagagctt
     1561 gtccagaaaa atactgtctt tactggtagt ttccacctga tttctagctg tgtttagggt
     1621 caaaaaattc aactcctcaa ccagtttatt gagcctctcc gtctggcgtc caatggttgc
     1681 tagataatga gcttgctccg actccttgat aatcccatcc aaaatccctt ctaccgtcgc
     1741 ttggatcgaa gtgataggag tcttaatatc atgcgacaac tgagcaatca tcaagccctt
     1801 ttctcgttcg ctttcttcca aggaatcaaa gcttacctgc aaatcatggg acatctcatt
     1861 aaaagtttgc cctaattgct gaaattctac aggaccttga acctccaaat ttgaaggaaa
     1921 atccttggcc gctacccgct tggcatgctc cttgagtttg cccaacgacg taaagactgg
     1981 caataggaga aagagactaa tcccagcacc gacaaggctg gcaacgatgg tcatcccaag
     2041 caaaaagtaa atctcgcctt tcgcaatcag cattttttga acagcccaaa aaacaaccaa
     2101 aatggttaag agggttgaaa taatatatcc aaccaaaata taacttttta gtttcatgtt
     2161 tgtcctctcg gtttctctat cttatatccc aacccccaaa ctgtcttaat agtgggcgtt
     2221 tggtcactac tatattttgc cagctcctgt cgaagagcat ggatatgcac attcaaggta
     2281 ttggtgtcat ccacgtagtc ttctttccag atcttttcat agaggtctgt cttggagaaa
     2341 actcgctctg gattgctagc taaaatccac agcaattcaa atgatttaac agttaaatcc
     2401 agcatttctt ctcctatttg aacttcatga ctactatgat tcatttttag attgccaagg
     2461 gaaatcagct ctgtttcgcc cccacgatga aggcggcgca aaatattgtg gacacgcaaa
     2521 actagctcac gtgggctaaa aggcttagca ataaaatcat ctgctcccaa gctcaggccg
     2581 taaatcttgt cctgttcact ggtcttagca gtaataaata ggaaaggctg ctctggtgat
     2641 aagtattgaa cctcactgat taaatcataa ccatccatcc gaggcatcat gacatctgtg
     2701 ataatcaaat caatcggttt tttcttgaac agctctagcg cttccagtcc atcatgggct
     2761 accaagacct gataacctgc ctgaattaag tatctctgat gaatatctgt gatttctacc
     2821 tcgtcgtcaa cgagtaaaat tgtctttccc atctgtctct cctttgataa aaacagtgct
     2881 atactgcttt aagtataaca ctatttttat tgttttacga tgattttaat ctgctattga
     2941 aagaaagtcc tgattgctga ttgagttgtt aaactaatct tagtcagccc actatccatc
     3001 atttatttcc ttagctgatt agttttcttc ttttttgttt ttcaaaccta gaccaccgag
     3061 taaacctgca agcgcaagcc caaggaaacc aatacttgcc attgatgttt gagtctcacc
     3121 agtatttggt agcatagctt tatcctctga catcatcgta tcagacatct tgttagcaga
     3181 agcagccatg ttttcacctg ccatcgtgtt ggtagaactt gtcatggtgt cagcaggcat
     3241 gctatctgta gtacctgtag catgattgtg attcatcgga gtcacgccag aaccagagtt
     3301 agaaggtgat aatgaaccat ttgctgtatc tgatgtttct ttaacattta tcttaatagt
     3361 gactttttta gttgctacga tgttgtccaa gtctggttta cc
//
LOCUS       DAVKHZ010000114         3383 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000114 DAVKHZ010000000
VERSION     DAVKHZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.114"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..911)
                     /gene="phtA"
                     /locus_tag="VWW99_001984"
     CDS             complement(<1..911)
                     /gene="phtA"
                     /locus_tag="VWW99_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9539774.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHV"
     gene            complement(1165..2898)
                     /gene="ptsP"
                     /locus_tag="VWW99_001985"
     CDS             complement(1165..2898)
                     /gene="ptsP"
                     /locus_tag="VWW99_001985"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9539775.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(2904..3167)
                     /locus_tag="VWW99_001986"
     CDS             complement(2904..3167)
                     /locus_tag="VWW99_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9539776.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT          888 a          730 c          640 g         1125 t
ORIGIN      
        1 acatggcgtt gactcaaagg cagtttgtag agctgtttca agagactatc aatgtcatta
       61 ctttgacttg cttgactgtt agtgttgctg ttgttgcttg tgttagtatt tgtagttcct
      121 ggattgctta cagaaggtac ccagtttgtt cttggagtgt tatcgctatt ttgtcggcga
      181 taggttctta aatttgacag attttcccga ccagatagga aggcttctgc agcagccaac
      241 tcgctagctg ataactcatt cttaggaatg taatggtaat gatctccatg aggaacgata
      301 taagcatcac cagtatcctc tatgatatca gaagcattaa agatataacc atcatctgtg
      361 gtgtagcgtc cctgtgaacg tgcaaaggct accgcaccat cgtttgctga agtccctcct
      421 tcacgatgct gactatgttc ttgtttttgc cgattgattt cttcttttgt acggacatta
      481 tccgcgtggg cagcatcctt aaggtaaaca tagtattttc catctacctt gataacatat
      541 ccacccttga cctcattaac aatatcctca tcttttagct tatagtttgg atctttcatg
      601 agtaattctt cactgaaaat cgcatcataa ggaaccttac cattgtaata atgatagtgg
      661 tcgccatgtg aagtgacata gccttggtct gttatcttga tgacgatttg ctcagcattg
      721 attccttcac gcttgctaac ctcatcagga gtcaaattct ccgttttttg cgtcgcttgt
      781 tttccatcta tataggaaac acgattattt tccttaaccg ttctagcttg atacagtccc
      841 aactcgtaag aacaaacact taaaatcaaa gctgccgcag aaccaacaag gtatttctta
      901 ttaattttca tcgaaactct ctttctattt gatatttcta gttgcaattc atttctaaat
      961 tgcagaaact cctctctatt acttaactgg ttaatagttt actcctaaat ctccaacttg
     1021 tcaagaattt tatgaaccat ttaagtattt tttgttctga ctactacttt ttcaaggttg
     1081 gactttctat aagaaagatt ttcttgaaaa ttctttttaa aatatcaaaa aaatccctgt
     1141 aactgagttg cagggacttt tcgattaatc aaaattaacg tattcttttt gaagttcaag
     1201 aacttcttcc attgttgagc attctgtaag ggcacggttt gcgtactctt ccatcttagc
     1261 tgtgtcgagt ttcttcatca agctgcgtgt acgaagtaca gacgttgctg acatagagaa
     1321 ctcatccaag cccattccga caagaagtgg aacagcttgt tggtcaccag ccatctcacc
     1381 acacatacca gcccatttac cttcagcgtg agctgctttg atcacattgt taatcaagcg
     1441 taggattgat gggttgtatg gttggtaaag gtatgaaact tgttcgttca tacggtctgc
     1501 tgccattgta tattggatca agtcgtttgt accaattgag aagaagtcaa cttctttagc
     1561 aaattggtct gcaagcatag ccgctgcagg aatctcgatc atgataccaa cttggatgtt
     1621 atccgcaact gcaacacctt cagcaagaag gtttgctttt tcttcatcaa agactgcttt
     1681 cgctgcacgg aattctttca agagcgcaac cattgggaac atgatacgca attgaccgtg
     1741 aacagacgca cgaagaagag cacggatttg tgtgcggaac atagcatctc cagtctcaga
     1801 gatagagata cgaagagcac ggaatccaag gaatgggttc atttcgtgag gcatatcgaa
     1861 gtaaggaagt tccttatctc caccgatatc cattgtacga acgacaacag gtttaccgtt
     1921 cattccttca agaacagcct tgtatgcttc atactgctca tcttcagttg ggaagtcttg
     1981 agaatccatg tacaagaact ctgtacggta aagtccaaca gcttctgcac cgttgttgtt
     2041 aacaccttca acgtcttttg gagtaccaat attggcagcc aactcgaagt gtttaccgtc
     2101 agcagtcact gtttgagcat ctttcaaaag tgcccattca gctttttgtt tcgcataggc
     2161 ttcaccagct gctttaaatt ctgccgcttg ttcatctgtt gggttgataa tcacttctcc
     2221 agtgatcccg ttaacagcaa ggatgtcacc gtctttaacg atttcagtga tgttgtttgt
     2281 acctaataca gcagcaattt caagtgtacg tgccatgata gctgagtggc ttgtacgtcc
     2341 accaatgttg gttacaaaag cttttacaaa gtttttgtcc aattgagctg tatctgaagg
     2401 agtcaagtca tgcgcaatca caatcacttc ttcattgata gaagctgggt ttggcaattt
     2461 tttaccaaga aggtttgcca atacacgttt tgtcacgtcg cggatatccg ctgcgcgttc
     2521 ttgcatgtat gggttgtctt ccatgccttc aaagatagtg ataaacatat ctgtaacttc
     2581 tttcagacct gcttctgcat tcactttctt cgcacggata gtttccttga tttggctgat
     2641 catttctggg tcagcaagaa ccattaagtg agcatcaaaa acttgagctg cttcttcacc
     2701 gagcgtacct actgctttct cgcgaataac agaaagctcg tcttgtgatg cctgtagagc
     2761 ggcatcaagg cgagcttctt ctgcgtttgt atcttcgact gtaatagtct caaatgacaa
     2821 atccggctga acgagtagat atgcttttgc aactgcaaca ccgtcagatg ctgcgattcc
     2881 tttaagcatt tctgtcattt cccttatgcc aatccttctt tttccattgt ttctgagatt
     2941 gcagcgatag cgtcatctgc atctgcacct tcagctgaga tagttacgtc agcaccttgg
     3001 ccaacaccaa gactcataac acccataatt gatttaaggt taactgattt acctttgtac
     3061 tcaagagtga tatctgaagc aaatttgcta gcagtttgta ccaacaatgt tgctggacgt
     3121 gcgtgaatac ctgtttctgc cactacgtgg aaatctttag aagccatagt ttgactctcc
     3181 ttttgttctt tctttttttg agttatatgt gataaccctt acaatttagt attataccat
     3241 cttctaggat ttttttcaag cattttctgg attttccttc aatttccttt cagataactt
     3301 gctgaaaatc ttctatccta gataacaaaa cacaggatat agtttcttta aaaacaattt
     3361 ataggataat cattgctatt tca
//
LOCUS       DAVKHZ010000115         3233 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000115 DAVKHZ010000000
VERSION     DAVKHZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.115"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(422..955)
                     /locus_tag="VWW99_001987"
     CDS             complement(422..955)
                     /locus_tag="VWW99_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9539777.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1044..1820)
                     /gene="recX"
                     /locus_tag="VWW99_001988"
     CDS             complement(1044..1820)
                     /gene="recX"
                     /locus_tag="VWW99_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9539778.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1858..3213
                     /gene="rlmD"
                     /locus_tag="VWW99_001989"
     CDS             1858..3213
                     /gene="rlmD"
                     /locus_tag="VWW99_001989"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9539779.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1036 a          556 c          575 g         1066 t
ORIGIN      
        1 tgagtttatt gaatctaaaa gtattgcttt atattttcaa aaacgattaa atgagctgat
       61 agattaaata tcattttctc tgttaagata ttgtttttaa aatattgtac taaatgattg
      121 atgctatgtg gaaatacaaa aaaatgtttt tgatacgaag ttgacctgta ttttttatac
      181 taatcatttt cgtatttttt gtattaaacg atataagttt gttgtaaact tacaaggaat
      241 aaagacatta aaaaataaca gtatatctat ttgttttata tattttacga attttgcata
      301 aatctctttc tagtaatgtg ttgtaactct gctataatag atttattcct ttttgtgttt
      361 acacaattta ttttatacta ccaaaaaaag gtcaggattt tgttcctgac ctttgacaac
      421 tttaccgatt ctttagttct acatagcgct tgtaccaaat gtttacatag gcttctgaga
      481 aaggaccacg tccattgtta atccaatcaa caagaatttt gacatgttct tttaaaatat
      541 agtccaagtc atcagaataa ttcattttgc gtttgtgacg ctcgtactct tcaacgtcca
      601 agagacgttt ttccccatct gtaaaaattt taacatccaa atcgtaatca atatacttca
      661 gtgcttcttc atccagatag taggggctag ccatattgca atagtaagaa gttccattat
      721 cacgaatcat ggcaatgata ttaaaccaat atttcttgtg aaagtaaaca atagccggtt
      781 ctcgagtgac ccaacgacga ccatcacttt cggtaacaag tgtatgatcg ttgacaccaa
      841 taatggcgtt ttctgttgtt tttagtacca tggtgtcccg ccaagttcgg tggagactcc
      901 catcatgctt ataactttga attgtaataa agtcgccttc ttttggaagc ttcataacta
      961 accaactttc tacaatttat aagtttatca tttactattg taccataaaa ttacctaaaa
     1021 tctgtgaatt tcacttggaa atattaaaga tattctctaa gagcgcttgc tatatccgaa
     1081 aaatcgtagc cctttcgtgc taaaacttga gttaaacgct gcttcagttc gtatccttca
     1141 tactttcgag catacttagt atattgctta tcaagttcct tgaagatgag ttcttgagtc
     1201 gtttcttggt caacttggct atctaagtcg tcaaaggcaa ttttagcatc agaataagag
     1261 aagcccttgt tagtcaagtt ctggataatc ttatcttgca gggcacgagc tggaagtttt
     1321 ccctcatatt ttttcaatag tttattggct acacgttgag caacttccga aaaatcaaaa
     1381 tcattcaaga tttcttctat agtagatttt gaaattcctt tttgtgctaa tttctgagtc
     1441 agtacataag gtcccttgtc tcctgaaagt tgattggcat tgatgatagc ataagcgtac
     1501 tgaccatcat taatccactt atcttcttta agattagcaa taacttgaga aacgatgttt
     1561 ttatcaatat cgtatttttt cagatattct ctgacttctt tttcagtgcg tgctttaaag
     1621 gataagtggt agagggccag attcttacca taagaaaatt gagcaaagtc ttgaatctct
     1681 ttcaattcct cttcgcttat caccttatct ctcgataaca taaaacgaac aattgtatct
     1741 tcggtgatat agcatttgtc gccattatca agctccatca gatagagtct ttttttcttt
     1801 tcaagttttg tgattttcat agctctatta taactcaaaa tgtgataaga taggggtatg
     1861 aatctgaaag tgaaacaaaa aataccatta aaaatcaagc gcatgggaat taacggtgag
     1921 ggaatcggct tttaccaaaa aacattagtc tttgtaccag gagctctcaa aggcgaagat
     1981 atctattgtc agattacttc tattagacgc aactttgttg aagcaaaatt actgaaggtc
     2041 aacaagaagt ctaaatttcg aattgtgcca tcttgtacta tttataatga atgcggaggc
     2101 tgccaaatca tgcacctgca ttatgataag cagctggagt tcaagacgga cttacttcat
     2161 caagcgctga aaaaatttgc tcctgcagga tatgaaaatt atgaaattcg tccaactatt
     2221 ggaatgcagg aaccaaaata ttacagagct aagttacaat ttcagacccg aaaatttaaa
     2281 aatcaggtca aggcgggctt atatgcacaa aactctcact atttagtaga gttgaaagac
     2341 tgcctggtac aagataagga aacccaagtg attgctaatc gcttagcaga attacttact
     2401 tatcaccaga ttccaatcac ggatgagaga aaagttctag gtgtccgtac tattatggtc
     2461 cgacgcgcga gaaagaccgg acaggttcag attattattg ttacaaaccg ccagcttaat
     2521 ttaactcaat tggtaaaaga gttggttaaa gatttcccag aagttgtgac agtagctgtt
     2581 aatacaaata cagctaaaac cagtgagata tatggtgaaa agacagagat tatctggggg
     2641 caagagagta ttcaagaagg tgtactcaat tatgaatttt cactatcccc tcgagctttt
     2701 tatcaactaa atcctgagca aacagaagtc ctctatagcg aagcagtaaa agcgctggat
     2761 gttgataaag aagaccattt gattgacgct tattgtggag ttggaacgat tggatttgcc
     2821 tttgcaaaga aagtaaaaac actcagaggt atggatatta ttccagaagc tattgaagat
     2881 gccaagcgaa atgctaaaag aatgggattt gacaatactc attatgaagc tggaacggca
     2941 gaagagatta ttcctcgttg gtacaaggaa ggctaccgag cagatgcttt gattgttgac
     3001 ccaccacgta caggtctgga tgataagtta ttagatacta ttcttactta tgtaccagaa
     3061 aaaatggttt atatttcttg taatgtttcg accttggctc gtgatttggt acgcttagta
     3121 gaagtctatg atcttcatta tatccagtcg gtcgatatgt tcccacatac agctcgaact
     3181 gaagctgttg taaaattaat aaaaaaagtt taaaaaaggt gttgacaaaa cat
//
LOCUS       DAVKHZ010000116         3162 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000116 DAVKHZ010000000
VERSION     DAVKHZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.116"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..314
                     /locus_tag="VWW99_001990"
     CDS             <1..314
                     /locus_tag="VWW99_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539780.1"
                     /translation="KKGQFDELVSNLNLSEQEQLDLLQQYKEEHPRRAKRGIKSAIIK
                     KVARFLAAKVGQKSVVEITDYLFEWQDNLEAGAENYLVQYGWDRNIAHWTIKTVSFIF
                     L"
     gene            801..1763
                     /locus_tag="VWW99_001991"
     CDS             801..1763
                     /locus_tag="VWW99_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEU9539781.1"
                     /translation="MEKWENQDKILLDKNKRGKDRNWRGRKLLSLKLADIFKELGYRE
                     TLIERVETCGDTLRFIRREDGSLRLYQAYFCKNKLCPMCNWRRSMKYSYQTSQIVDEA
                     IKEQPKGRFLFLTLTVKNVPGKELNATISQLTQSFDRLFRRAKVKKNLIGFLRSVEVT
                     HNQEEETYHPHIHVLMMVKSSYFSGAGDNYVSQEEWGRMWEQSLKVDYVPIVDIRSVK
                     EIGKGLKGAILETAKYPIKPIKLDVENKQVVGDLYNGLYRKRQLGYGGLFKEIRKRLQ
                     LSNVENGDLVYISDDNDEMSKGTKIVAIWNATKQNYFVKNKGWN"
     gene            2208..2537
                     /locus_tag="VWW99_001992"
     CDS             2208..2537
                     /locus_tag="VWW99_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539782.1"
                     /translation="MSISFRTNTLMMNIAYKNRDLSDEEFKEEAHQFRDKEIKILEEK
                     LKELNISCKVVGINSIKDMNEYKEIMENVNQAKEELKRIDELIAVRSSRIDFLEDKTL
                     RITGNQR"
     gene            3000..>3162
                     /locus_tag="VWW99_001993"
     CDS             3000..>3162
                     /locus_tag="VWW99_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539783.1"
                     /translation="MNKKFLKCGTLFLISCSILGSTIPAVTVFSDEVTITYNSENNSE
                     KNELYNQLSA"
BASE COUNT         1116 a          364 c          650 g         1032 t
ORIGIN      
        1 aaaaaaaagg acagtttgat gagttggtaa gtaatttgaa tttgagcgaa caagaacaat
       61 tagacttgtt gcagcagtat aaagaggaac atcctcggcg agctaaaagg gggataaaat
      121 cagctataat aaaaaaagtc gctcgtttcc ttgctgctaa agtaggacaa aaaagtgtag
      181 tggagataac tgactattta tttgaatggc aagacaattt agaagctgga gcggagaatt
      241 atcttgtaca atatggttgg gatagaaata ttgctcactg gacaataaag acagttagct
      301 ttattttctt ataagtcatt atgaataaag cttggttatt ttggagtgtc atagtcttat
      361 atttttttat aaagtttttt gataaagttt tagatataaa gctttttggt tctattcaaa
      421 tatggctaga taatctccct attccgatga aaatactgtt aacaatagca ttagttttgt
      481 ttttattcat tgtatttcca tatagaggaa aacggtaaaa acggtaatta gatatataaa
      541 aggagctgaa agctccgtaa aaaacctact cgaagagtag gttttttctt atcttgatac
      601 tagtaggaaa taactctcat gctattttta gggtttcgac agggtctaaa cccttgatac
      661 tactgacttt ttaggtaaaa aaatgccctt gacttttttc aacggatctt ttataattta
      721 gttgttcaga aaaaattata aaaagagaaa aaaggcaaag gtatttttgt atttctttgt
      781 aattacaggt aaattataac atggagaagt gggaaaatca agataaaatt cttttagata
      841 aaaataaaag aggcaaggat agaaactggc gaggacgaaa attattaagt ttgaaattgg
      901 cagatatttt taaagagtta ggttataggg aaaccttgat agaacgcgtt gagacttgtg
      961 gagatactct tcgatttatt cgtcgagaag atgggagttt gcgtttatat caagcctatt
     1021 tttgtaagaa taaattgtgt cctatgtgta attggagacg gtcaatgaaa tattcttatc
     1081 aaacatctca gatagttgat gaagcaatta aagaacaacc aaaagggcgt tttcttttct
     1141 tgactttgac tgtgaaaaat gttccaggta aagaattgaa cgcgacaatt agccaactaa
     1201 ctcagtcctt tgaccgtctt tttagacggg caaaggtaaa gaaaaatcta atcgggtttt
     1261 tacgttcggt tgaggtgact cataatcaag aagaagagac ctatcaccct catatccatg
     1321 ttttgatgat ggttaagtct agctatttta gtggggctgg agataattat gtatctcaag
     1381 aagaatgggg gcggatgtgg gaacagtcgc ttaaggttga ctatgtgcca atagttgata
     1441 ttcggtcagt taaagaaata ggaaaaggtc ttaaaggggc aattcttgaa acggcaaaat
     1501 atccaattaa gccgattaag ttagatgttg aaaacaagca agttgttggt gatttgtata
     1561 atgggttata tcgtaaacgg caattgggtt acggtggatt gtttaaagaa attcggaaga
     1621 gattgcaact gtctaatgtc gaaaatggag atctggttta tatttctgat gacaatgatg
     1681 aaatgtcaaa aggtacaaaa atagtcgcga tttggaatgc gactaaacaa aactattttg
     1741 taaaaaataa gggttggaat taaagtatca actcttttat tataacataa aaaaagagta
     1801 aaatagactt gatttttttg agtttagatg tatattgaag gtgtgaaaat taaaaagata
     1861 gctatgtttt tttctgggta tacttgttgg ggtatttatt gttctatttt ttagaaatta
     1921 ttttgtttta cttttggaat atatttttgg ttaaaaagca ccctctaaaa agggtgcttt
     1981 ttgtgcggtt attcgcacga aagttctctg caagagttcc tcggaagagc gtttgtattt
     2041 tgattgtacg tcaaaactat aaaaacattt actctttgtc aaagagtagc agaaaagcta
     2101 gaataaaatt tttaatttta ttctagctaa tagtgcaagt aatagctttt gtagcttgcc
     2161 tcttgtccgt tattcagaca agaggcagga gtttgtattt gttctaaatg agtatttcat
     2221 ttagaacaaa tacattgatg atgaacattg cttacaagaa tagagatttg agtgatgaag
     2281 aatttaaaga agaagctcat cagtttagag ataaagagat aaaaatactc gaagaaaaat
     2341 taaaggaact taatatttct tgtaaggttg ttggaataaa tagtataaaa gatatgaacg
     2401 agtataaaga gattatggaa aatgttaatc aagcaaaaga agaattaaag agaatagatg
     2461 aattaatagc cgtcaggagc tccagaattg attttttaga ggataaaacc cttagaatta
     2521 caggaaacca aagatgagct taaaaacgaa atggagagcc taaaaagaga tttatctttt
     2581 tttagaaacg aaaatactcg actgaaaaat gtagtttcag attgtatcaa ggtagtattt
     2641 ttggggtatt tttagtagaa agtatattgt aatttctgag cgatttatgc cgtgaaagct
     2701 acttgaccgt aagctagatc aaagtacgct agtcagggac gagccgaaag gctgtagcgt
     2761 ttcggagcgg cacggacaaa gtacgtcgac ctaggttact tgcggtcaag tagaccatgg
     2821 aataaaacaa gcgggtatga tataaaaaag ttatcggcaa accgaaatat agacttgttt
     2881 ttttgtttat aaaaatgtat tattatattg agaataagat tgtttaattt atttagagag
     2941 gagtgtaagt tgatcagtaa acgattttaa ttctgccttt aaataacaag gagtttgtta
     3001 tgaataaaaa atttttgaaa tgtggtactt tatttttgat ttcttgttct attttaggaa
     3061 gtactattcc tgcagttaca gtattttctg atgaagtaac aattacttat aattcagaga
     3121 ataattcaga aaaaaatgaa ttatataatc aactttctgc tg
//
LOCUS       DAVKHZ010000117         3080 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000117 DAVKHZ010000000
VERSION     DAVKHZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.117"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            280..1566
                     /locus_tag="VWW99_001994"
     CDS             280..1566
                     /locus_tag="VWW99_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9539784.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAAIEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1581..1892
                     /locus_tag="VWW99_001995"
     CDS             1581..1892
                     /locus_tag="VWW99_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539785.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2138..2866
                     /locus_tag="VWW99_001996"
                     /pseudo
     CDS             2138..2866
                     /locus_tag="VWW99_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          956 a          537 c          671 g          916 t
ORIGIN      
        1 atccttttcc attataggag caacagagtg ggagtagtca tctaaggact aatttatgta
       61 tttttacgag tcagtatctt tggatactgg tttttacttt tctagacttt ttgactactt
      121 gttaaaactg ggataatttt cgactgttta acagttatta tgcaaagtct aaaagattag
      181 aattgtcaaa acaatccgtc taggcttgat tttatccttt atttactata aaatgagaag
      241 gaaaaatgtc aaacttttat attgcaaata ggagaaatca tgacaaaaac attaaaacgt
      301 cctgaggttt tatcacctgc agggacttta gagaagctaa aggtagctgt tcagtatgga
      361 gcagatgctg tctttatcgg tggtcaggcc tatggtcttc gtagccgtgc gggaaacttt
      421 actttcgaac agatggaaga aggcgtgcag tttgcggcca agtatggtgc caaggtctat
      481 gtagcggcta atatggttat gcacgaagga aatgaagctg gtgctggtga gtggttccgt
      541 aaactgcgtg atatcgggat tgcagcagtt atcgtatctg acccagcctt gattatgatt
      601 gcagcgattg aagcaccagg ccttgaaatc cacctttcta cccaagccag tgccactaac
      661 tatgaaaccc ttgagttctg gaaagagcta ggcttgactc gtgtcgtttt agcgcgtgag
      721 gtttcaatgg aagaattagc tgagatccgc aaacgtacag atgttgaaat tgaagccttt
      781 gtccatggag ctatgtgtat ttcatactct ggacgttgta ctctttcaaa ccacatgagt
      841 atgcgtgatg ccaaccgtgg tggatgttct cagtcatgcc gttggaaata cgacctttac
      901 gatatgccat ttgggaaaga acgtaagagt ttgcagggtg agattccaga agaattttca
      961 atgtcagccg ttgacatgtc tatgattgac cacattccag atatgattga aaatggtgtg
     1021 gacagtctaa aaatcgaagg acgtatgaag tctattcact acgtatcaac agtaaccaac
     1081 tgctacaagg cggctgtgga tgcctatctt gaaagtcctg aaaagtttga agctatcaaa
     1141 caagacttgg tggacgagat gtggaaggtt gcccaacgtg aattggcaac aggtttctac
     1201 tacggtatac catctgaaaa tgagcagttg tttggtgctc gccgtaaaat tcctgagtac
     1261 aagtttgtcg ctgaagtggt ttcttatgat gatgcggtac aaacagcaac tattcgtcaa
     1321 cgaaacgtca ttaacgaagg ggaccaagtt gagttttatg gtccaggttt ccgtcatttt
     1381 gaaacctata ttgaagattt gcatgatgcc aaaggcaata aaatcgaccg cgctccaaat
     1441 ccaatggaac tattgactat taaagtccca caacccgttc aatcaggaga catggttcgt
     1501 gcattaaaag aaggactcat caatttttat aaggaagatg gaaccagcgt cacagttcga
     1561 gcttaagaaa ggaaaaggaa atgatagagg cacagggttt cttagtggat aagcaaacaa
     1621 gatgcattca ttaccatagc aagctggata ttattgcttt acaatgctat gattgtaaaa
     1681 agtattatgc ttgttatcgg tgtcatgatt cattagaaaa tcaccctttt gagccgtatc
     1741 ccttatcttt gatacaggat aagcctattt tatgtggtgt ttgtctaaaa ctactaacat
     1801 ataagcaata taaagaaagt ttaagttgcc ccttttgttt ttctcgcttt aatccaggtt
     1861 gccaaaatca taaggaacgc tattttaaat agcaaatcat ctagttttga agtaggagaa
     1921 aactcaattc caagagaaaa tgaagtaaat cttcccacaa taaaacgcat aatatcaaga
     1981 ttgttcaata cctgatacta tgcgttttta agattttaaa gacttttttc ctttatctgg
     2041 tattttgact acttgttaaa actgggttaa ttttcaactg tttaacagtt attatgcaaa
     2101 gtctaaaaga ttagaattgt caaaacaatc cgtctagagt atgcgtgatg ccaaccgtgg
     2161 tggatgttct cagtcatgcc gttggaagta cgacctttac gatatgccat ttgggaaaga
     2221 acgtaagagt ttgcagggtg agattccaga agaattttca atgtcagccg ttgacatgtc
     2281 tatgattgac cacattccag atatgattga aaatggtgtg gacagtctaa aaatcgaagg
     2341 acgtatggag tctattcact atgtattaac agtaaccaac tgctacaagg cggctgtgga
     2401 tgcctatctt gaaagtcctg aaaagtttga agctatcaaa caagacttgg tggacgagat
     2461 gtggaaggtt gcccaacgtg aactggctac aggattttac tatggtacac catctgaaaa
     2521 tgagcagttg tttggtgctc gtcgtaaaat tcctgagtac aagtttgtcg ctgaagtggt
     2581 ttcttatgat gatgcggcac aaacagcaac tattcgtcaa cgaaatgtca ttaacgaagg
     2641 ggaccaagtt gagttttatg gtccaggttt ccgtcatttt gaaacctata ttgaagattt
     2701 gcatgatgcc aaaggaaata aaatcgaccg cgctccaaat ccaatggaac tattgactat
     2761 taaagtccca caacctgttc aagctggaga catggttcga gctcttaaag aggggcttat
     2821 caatctttat aaggaagatg gaaccagcgt cacagttcgt gcttaatgta gttgtttagt
     2881 tttaaaaaat tatgcaaagc tccatataca acacttaaac gagattaaag aatggcgaaa
     2941 tcccctgatg cgcaagagat tagctgtctt ttttattttt taagtgataa agtcggagct
     3001 taggtatcaa agcctatcaa attaaacaaa gaagcgatgt cttagatatt ttgaaaaaaa
     3061 ttaataagca gaaaactctc
//
LOCUS       DAVKHZ010000118         3077 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000118 DAVKHZ010000000
VERSION     DAVKHZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.118"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..470
                     /locus_tag="VWW99_001997"
     CDS             <1..470
                     /locus_tag="VWW99_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickase"
                     /protein_id="HEU9539786.1"
                     /translation="FNLTNYLLTYSEIQKEKSKYLKGYAKTNKEKYDFKKLTSAYSYL
                     RKNNIETIGQLQTKIETLKSNSYRLNKKAKTIHKEMEDVEKKILYYEIYKAKKEVYEE
                     YQKKNIFTKEAFYNKHKKDIDQYKVVSGKLKKLLSDKEKLSPKKWNEEKILLM"
     gene            796..969
                     /locus_tag="VWW99_001998"
     CDS             796..969
                     /locus_tag="VWW99_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539787.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            1155..1682
                     /locus_tag="VWW99_001999"
     CDS             1155..1682
                     /locus_tag="VWW99_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU9539788.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1809..2285
                     /locus_tag="VWW99_002000"
                     /pseudo
     CDS             1809..2285
                     /locus_tag="VWW99_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
     gene            2877..3038
                     /locus_tag="VWW99_002001"
     CDS             2877..3038
                     /locus_tag="VWW99_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539789.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT         1493 a          392 c          511 g          681 t
ORIGIN      
        1 tttttaatct aacaaattat ctattaactt attcagaaat tcaaaaagaa aaaagtaaat
       61 atctaaaagg atatgcaaaa actaataaag aaaaatatga cttcaaaaag ctaacaagtg
      121 catatagtta tttaagaaaa aacaacatag aaaccatagg tcagttacaa acaaaaatag
      181 aaactttaaa gtctaatagt tataggctaa ataaaaaagc aaagacaatc cataaagaaa
      241 tggaagatgt agaaaagaaa attctatact atgaaatcta caaagccaaa aaagaagttt
      301 atgaagaata tcaaaagaaa aatatattca caaaagaagc attttataac aaacataaga
      361 aagatataga ccaatataaa gtagtaagtg ggaaattaaa aaaactccta tcagataagg
      421 aaaaactaag tcctaaaaag tggaatgaag aaaaaattct tttaatgtaa aatcttgaag
      481 aaataaataa agaaaaagac aagataaaag atgaatacca ggaaattaat catataaaat
      541 attcagtaga ttttgtaaac aaagaacttg gtatagattt atccatagaa atagataagc
      601 taataaaaca gggagaaaaa ccaagtgtaa tagcacagat taaaaagttc caagaccaag
      661 tcaataaaga taatgaatac agagaaatga tgaaaaacaa gaaaatggat caagaaagat
      721 aagacctggt aaaaatatct tatccaagct ataatatgac caaacaacta aagtcagatc
      781 taggaggtaa tagagatgaa taaaaggcaa gagctaatag atgaactcat aaaagcaaat
      841 gaagatggaa catacaaaac ctataaaagc acagaagaaa taaaagcaat gaacaatgaa
      901 gaaatacaga ttatatattc caacatgaaa aactatctat cagacaaaag aacacacata
      961 aactattaaa ggtggaaagg tagtgtaagc tatttttaaa agtataaaag gtacaaagac
     1021 ctaagaaata tataaaaaca tcaaaataaa gtattccacc accaagaaca ataaaataaa
     1081 aacaagcaaa gggaagtata gaaaaattaa aacctatact tccctttttt aattcaaaca
     1141 aaggagagaa aagcatgata aacgaagaaa taagcaagga agcaggtcaa gcagcacaaa
     1201 ccataatatc atacacaata aaggcaacaa aagaatcaat caatttagaa aaagaaataa
     1261 gaaaaaagat gaatgaaact ttagaaaaag caaatggaaa cttaaaaagt cttatgggcg
     1321 atgaaatgaa aataaaagat ctctacaaga aaggacaact agaaaatata agcatagatc
     1381 aaatcgacct caaagactta aaaaaagaac taaacaaact tggagtaagt ttctcagtaa
     1441 tgaaaaacaa agaaagcaaa aactatgaaa tattcttcca agccaaagac ataaaagtaa
     1501 tggaatatgc ctttaagcaa gtcatagcca aggaaaataa aaaagaaaaa gaaagtatcc
     1561 taaaacaaat aaagaaatac aaagacctat ccaaaaacaa agataagaca aaagaaaaag
     1621 gaaaaaggaa agtaaaagaa aaagtaaaac caaacaaaaa agatatgacc agagaaatct
     1681 aaagggagta acgaaaagtt acacccttaa ataaccataa taacaagact tccgaaaaac
     1741 agaagtccag aattccgaaa atcggaaatc aagaatttcg attatcgaaa atcaagaaag
     1801 gagcagatat ggcaacaaac aaaagatact attggataaa attaaaagaa gaatttttca
     1861 cagacaaaag gataaaaagg ttaaggagaa tatctggagg agatacctat acgataatct
     1921 acctaaaact tctactacta agcctaaaag atgagggcaa actctattat gacggagtag
     1981 aaagtgattt tataaaagag ttagcactaa ccatagatga aacagacgat gatgtaatgg
     2041 taacagtaaa ttatttaatc aatcaaggct tattagaagt tgtaacagaa aatgatgaat
     2101 actacctaac tgaaatacca aacttaatcg gatcagaaac agcctgggca gagaaaaaaa
     2161 gaagatacag acaaaataaa cagaggacat tgtccttaat gagtccaacc catgtccgac
     2221 aagagataga gatagataaa gagatagaga tagataaaga gagagaggga gagatagata
     2281 aaaagaggtg gaaaggcact attaaagact ttaagtctaa aaaggtatca aagtatgcca
     2341 gagataaaaa aatataaata tgacacttta aaggcgatta gaaaattaaa gaaatgctag
     2401 aagactttta gatgacagtt accaagaaag acttcttttc actgttaagt ataagagggt
     2461 aatttataga attttgttac atcaagataa atattttctg atggtcaaat tttaaaaaga
     2521 ttaataactc tcgataaatt ttgaacctta acaattgaat agaccaagac aagacgttaa
     2581 ttatttttgg agcgtaataa cctatccgtc aagatctttc ggctgaataa ttcggcaaaa
     2641 aaagttctgc taagcaaaaa gaaagtgagc gataagtaag agtttgaata tagggaggga
     2701 taccctatcc gctatgaaga aaatgaggat aatgatactt ctaaggttga agccggctca
     2761 tccggaatag aggcggaggt gagattccta tgttgctaat ccaaagcact tgtcaatgtc
     2821 aaaaaactta attaaacaaa taattattag atataaaact ttagaggagg tatatattgg
     2881 acaatgactg gaatggattg gctgatttaa tagcaaatct gattgcaaaa tatgctggtg
     2941 ctttagattt agataatcta cccgatccaa cgccagcaaa aaatcaagag atgaaaaata
     3001 gttttgacat ggcaaaaaca caaattgaga cggactaata aaagtgttat aatatacttg
     3061 aaatgatagt ccaaact
//
LOCUS       DAVKHZ010000119         2966 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000119 DAVKHZ010000000
VERSION     DAVKHZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.119"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            424..1044
                     /locus_tag="VWW99_002002"
     CDS             424..1044
                     /locus_tag="VWW99_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9539790.1"
                     /translation="MKDVVYSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLIN
                     GSLKPQKGYVNLLSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVL
                     KKVNLYEELEKIDILNYQCGENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDN
                     DNSNSIHDILFNLGISFIEVSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            1059..1799
                     /locus_tag="VWW99_002003"
     CDS             1059..1799
                     /locus_tag="VWW99_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539791.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            1802..>2966
                     /locus_tag="VWW99_002004"
     CDS             1802..>2966
                     /locus_tag="VWW99_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9539792.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYCKSY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMA"
BASE COUNT         1018 a          369 c          523 g         1056 t
ORIGIN      
        1 aaagaaattt ttaatggttt taagacatta aaatcttatt ctgttactaa agaaataatt
       61 agtttgtcgg ataaaaagtt ggataaacta gaagattcta cttttaattt gaaacgatca
      121 gaggttcttt caaaattggt tgcagtacta atttcaggtt tttgctttct agttcccttg
      181 gttgtaggat gttattttgt aatttatcat aaaagccttt cttttagtga actgataggt
      241 attttcttag caaacgataa agttttaggg ccgatacaat caattgccta ttcattaaat
      301 aagataaata caaccaaaga tttaaggaaa ccgtttttaa aatacttaag tggagagaag
      361 aattttatag acgctgaaca tgataataac ggactgtata cttcatcaat agatgagata
      421 cacatgaaag atgttgtata ttctattaca ccagaaaata aattaagtat tgacttctca
      481 tttaagtcac catttagggt attattaaca ggaacttctg gtagtgggaa aacaacgatt
      541 ttaaatttaa ttaatggttc tttaaagcca caaaaaggtt atgtaaattt gttatcacat
      601 gggaaaaaga gttcagattc aataccaaca gttgatcaga caccatatat ttttgacact
      661 actattcgtg agaacgtaac tttatttcaa aatgaatatt tttcagatga tcagataatt
      721 gaggtgttaa aaaaggtaaa tctatatgaa gaattagaaa agatagatat actaaattat
      781 caatgtggtg aaaatggtag taatttgtct ggaggtcaaa aacaaaaaat agctttagct
      841 agagctctga ttagaaataa taaagtgtac ttatttgacg aaatatcagc taatttagat
      901 aatgataatt caaattccat acatgatatt ctgttcaatt taggtatttc atttattgaa
      961 gtttcacatc attatgactt aaatgacaag agatacactg atatatataa attggaaaat
     1021 ggaacacttt tcaaaattaa gtgattggag gcaagaatat ggataaagat tatatattaa
     1081 aagtgaaagg gctgtatcat caatttttac taggaaataa taaaacgttg caagtgctga
     1141 aaaatgtttc tctttctgct tcgagaggag aatttataag tattctagga attagtggtt
     1201 ctggaaagtc aactttatta aaatgtattt ctagtttgct tgaaccaaca agtggggaag
     1261 taattttaaa tggaatcaac ccctataaaa tcagaaatgc aaaattgtca agtataagac
     1321 gtaacgaagt atcttttata tttcaagcat acaatttaat accttccctg ccggtaatag
     1381 aaaatatagc acttcctttg cgattatcac aaaaaaaatt aactattaaa aatgtagaaa
     1441 acttactcaa aagaatgaag tttaatgctg gcttaaacga ttttgttgga actctgtctg
     1501 gtggagaaca acagaaggtt gctatagcta gagcggttat tgctgatagt gatataatat
     1561 ttgctgatga gccaactggg gctttagaca gcgtttctcg tgaagtaatt tttgaattat
     1621 tgagagagtt agtaggggcg ggtaagtgtg taattatggt aacgcacgat atagaattgg
     1681 cctcgaaaac tgatcgtgca ttaatattga aagatggaaa aattttcaaa gaacttcata
     1741 gacctagcgg ggaagagttg tataaaatct tagaggtaca atcaactacg gaggaataga
     1801 gatgctcaga ttagtttact atcaattttt acataataag aaacaatggt taggcgtttc
     1861 accagtaatt tttgtttcga gtttagttat gggattggct gttaatgggg taattaatgt
     1921 tgagaataat tcgcaagtat ttgtcggact tccagatcct aaaccaattt ttatgtttcc
     1981 gatagtattc ggaggagtaa cattattctt tgtgctctct aacataataa atatgttggt
     2041 tgaaattttt agagatgatt acgaacttct ggaggtgttg ggagcaagtc gccttcaact
     2101 atcatttctt gtggggggac agatttttat catttcctcg attattagtt ttattgcata
     2161 tctatgctct atatttgtta cgtcaaatta ttattatttt ttacaatact ttttcgggga
     2221 aaatatatta cctgatatcc aatttcaaac gagtgctgtt ggatgtatca ttacagtagt
     2281 gttgatttca tttttagcct ttctaagtgg ttgcttttat acgttcaaga agattagaaa
     2341 taggaagagt tctaaaataa ggcatgttct ttcaatagta aaaagaatac tgctattggc
     2401 tggttttagt gttatctggt tgctttcttt gcaacaaatt ttccaagata gcacaatttt
     2461 agcgaaggcc cagataatat tcaatattgt aatcttagat attgtgatta tttatcaatt
     2521 atctcctttt atacagtcat gttttatcaa actactttct ataattatat ttagaaataa
     2581 ttttatgttc attgtttcga agtggaattt actatattgt aagtcttata taaaatctat
     2641 cagtgctgca attacaggag ctatattact aatttctagt tttcaaatga tttctcagaa
     2701 tatactatct caatttcaag atgacagtga tttagagcta aaagtagcat ttattgtcta
     2761 tgtaggagca ccaattttga tagtacttgc aaatattatt tcaattgcat ttttgtcatc
     2821 gcatcaagaa aggattgaga ttcagcaatt tgaaatttta ggaacttcta attaccagat
     2881 ggttaagatt aaagttggtg aggctatatt tttgacattt gttacttcat taattgcatt
     2941 tttacttaac attaaaatta tggctt
//
LOCUS       DAVKHZ010000120         2920 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000120 DAVKHZ010000000
VERSION     DAVKHZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.120"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            312..1658
                     /gene="gdhA"
                     /locus_tag="VWW99_002005"
     CDS             312..1658
                     /gene="gdhA"
                     /locus_tag="VWW99_002005"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9539793.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2164..2334)
                     /locus_tag="VWW99_002006"
     CDS             complement(2164..2334)
                     /locus_tag="VWW99_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539794.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2347..>2920)
                     /locus_tag="VWW99_002007"
     CDS             complement(2347..>2920)
                     /locus_tag="VWW99_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9539795.1"
                     /translation="KIPSRLYKIDTGKVLFDGVNINQIDKKILSQNLGVVPQDSFLLN
                     RSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIA
                     LARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIVAHRLSTIKDADVIFV
                     MKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
BASE COUNT          925 a          545 c          488 g          962 t
ORIGIN      
        1 ttttagtctg gtataaatat tgctttcctc caaaatggtc atagttttac tggcaaatct
       61 aacatatcac ggataaatta acaagtgatt tctgaattgc taaacatttt cttttcttat
      121 agcatacttt aagattttgt ctttgagaaa gatatttcca agaaaaacgt tcgttttttt
      181 atctattttc aaaaaaacca aaaaactgaa ttgaaagaat ttttagaaaa tttctgtttt
      241 ttctttacag tgaaaaaaaa ttctgctata atgtcttatg taataaaata tgacaataaa
      301 aggagaacga tatgacatct gctaaagaat atatccaaag cgtgtttgaa actgtgaaag
      361 ctcgtaacgg gcacgaggct gaattcctcc aagctgttga agaatttttc aacactttag
      421 aacctgtatt tgaaaaacac cctgagtata tcgaagaaaa tatcttggca cgtattactg
      481 agcctgagcg cgtggtttct ttccgtgttc cttgggttga ccgtgatgga aaaattcaag
      541 taaaccgtgg ttaccgtgtt caattcaact cagctgttgg accatacaaa ggtggacttc
      601 gtttccaccc aactgtaaac caagggatct tgaaattcct cggatttgaa caaatcttta
      661 aaaatgtctt gactggactt cctatcggtg gaggtaaagg tggatcagac ttcgatccta
      721 aaggtaaaac agatgctgaa gtgatgcgct tctgccaaag cttcatgact gaattgcaaa
      781 aacacatcgg accatcactt gacgtacctg ctggtgatat cggtgttggt ggacgtgaaa
      841 ttggttacct ttacggtcaa tacaaacgtc ttaaccaatt tgatgctggt gtcttgactg
      901 gtaaacctct tggatttggt ggtagcttga ttcgtccaga agcaactggt tacggtttgg
      961 tttactatac tgaagaaatg ctcaaagcta acggtaacag ctttgctggt aagaaagtcg
     1021 ttatttcagg ttctggtaac gttgctcaat atgctcttca aaaagcaact gaactcggtg
     1081 caactgttat ttctgtttct gactcaaatg gttatgtcat cgatgaaaat ggtatcgact
     1141 tcgatctttt ggttgatgtt aaagaaaaac gtcgtgctcg tttgactgag tatgcagctg
     1201 aaaaagcaac tgcaacctac cacgaaggta ctgtatggac ttacgctgga aactatgaca
     1261 ttgctcttcc atgtgccact caaaacgaaa tcaacggtga agcagctaaa cgtttggttg
     1321 ctcaaggcgt tatctgtgta tctgaaggtg ccaacatgcc gagcgacctt gatgccatca
     1381 aagtttacaa agaaaatggt atcttctacg gacctgcaaa agctgccaac gctggtggtg
     1441 tagccgtttc agctcttgaa atgagccaaa acagtcttcg cctctcatgg actcgtgaag
     1501 aagttgatgg ccgtctcaaa gacatcatga caaacatctt caacacagct aaaacgactt
     1561 cagaaacata cggtcttgat aaagactacc ttgcaggagc taacattgct gcctttgaaa
     1621 atgtagcaaa tgccatgatt gcacaaggta ttgtttaaga tttatttact caatcctcaa
     1681 tcactacgat tggggatttt tagcttgaat taaaaaattg gaaaatctaa ttaaataaaa
     1741 tgaaatgaaa taaaaactga acaaattgat tgcggaattc aaaccaattt ctaacatatt
     1801 tctagaaatt aatttgactt ttctaatcga tttgttcata tcttatttca atttactata
     1861 cttacttgac cctttttata ctcaatgaaa atcaaagagc aaactaggaa gcgagccgca
     1921 ggctgtactt gagtacggta aagcaaagct gacgtggttt gaagagattt tcaaagagta
     1981 ttaaacaaaa agaaaaaccg ctactcctaa gaatagcgat ttaatcatgt ttttaagcta
     2041 ctactgtagt tgctctatta tttaagtgtt atgaataact tcaatatctt taaagctttt
     2101 tgttagattt ataatttcaa ctatagccat ctctactcct tagtttacag caaaaaatta
     2161 tgcttaattc ttattgtttt tgtaaacaat cagcgaaccc aatactattc cattcaacaa
     2221 gcctaaagaa ataccatatt caactggaaa atctaaaaaa attgcaaaca ctattccaat
     2281 catcacccct agtgaggctg ttgttaaaat tacatattca ttttctttct tcattttctt
     2341 tacacctcat ttcctttttg tatataagct gtagtactct ccaccaagat ccattaagta
     2401 cttatgattt cctgactcaa caatcttacc acctttcatt acaaaaataa catccgcatc
     2461 cttaatcgtt gacaatctat gagctacaat tatttgagta cagccctgac tttgtatata
     2521 ctttgttatt ctttcctcat taatagtgtc taatgcacta gttgcttcat ctaaaattac
     2581 aatactagga ttatttatta atgcacgtgc cagtgctatc cgttgccttt gcccacctga
     2641 aatatttgac cccatctctg agatgatagt attaaatttc atcggcatag ccatgatttc
     2701 atcatagatt tgaactgctt tacaaacttc ctctatcttt tgtgaagtaa cttcgtgctt
     2761 taaagttata ttatcaagaa tacttcggtt caataaaaat gaatcctgtg gaactactcc
     2821 taaattttga cttaatattt ttttatctat ttgattaata tttacaccat caaataaaac
     2881 ttttcctgta tcaatcttat ataatcttga tggtattttt
//
LOCUS       DAVKHZ010000121         2801 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000121 DAVKHZ010000000
VERSION     DAVKHZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.121"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..2626
                     /locus_tag="VWW99_002008"
     rRNA            <1..2626
                     /locus_tag="VWW99_002008"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2703..>2801
                     /gene="rrf"
                     /locus_tag="VWW99_002009"
     rRNA            2703..>2801
                     /gene="rrf"
                     /locus_tag="VWW99_002009"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          791 a          566 c          817 g          627 t
ORIGIN      
        1 tttactcttc ggggttgtag gactgcaatg tggactcaaa gattatagaa gaatgatttg
       61 ggaagatcag ccaaagagag taatagcctc gtatttaaaa tagtctttgt acctagcagt
      121 atcctgagta cggcgggaca cgtgaaatcc cgtcggaatc tgggaggacc atctcccaac
      181 cctaaatact ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac
      241 cccgggaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt cgagcccgtt
      301 aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgttatg atgcgaggtt
      361 aagttgaaga gacggagccg tagggaaacc gagtctgaat agggcgcctt agtatcatga
      421 cgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgcggt aagacgcact
      481 ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta gcggagaaat
      541 tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct agcgtcgaca
      601 ttagagattc ttggaggtag agcactgttt gggtgagggg tccatcccgg attaccaatc
      661 tcagataaac tccgaatgcc aatgaattat ggtcggcagt cagactgcga gtgctaagat
      721 ccgtagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata attgttaagt
      781 ggaaaaggat gtggggttgc acagacaact aggatgttag cttagaagca gctattcatt
      841 caaagagtgc gtaatagctc actagtcgag tgaccctgcg ccgaaaatgt accggggcta
      901 aaacaattta ccgaagctgt ggataccttt ataggtatgg taggagagcg ttctatgtgt
      961 gatgaaggta taccgtgagg agtgctggaa cgcatagaag tgagaatgcc ggtatgagta
     1021 gcgaaagaca ggtgagaatc ctgtccaccg taagactaag gtttccaggg gaaggctcgt
     1081 ccgccctggg ttagtcggga cctaaggaga gaccgaaagg tgtatccgat ggacaacagg
     1141 ttgatattcc tgtactagag tatgtagtga tggagggacg cagtaggcta actaaagcag
     1201 acgattggaa gagtctgtct aagcagtgag gtgtgaattg agtcaaatgc ttaattctat
     1261 aacattgagc tgtgatgggg agcgaagttt agtagcgaag ttagtgacgt cacactgcca
     1321 agaaaagctt ctagcgttta aacatactct acccgtaccg caaaccgaca caggtagtcg
     1381 aggcgagtag cctcaggtga gcgagagaac tctcgttaag gaactcggca aaatgacccc
     1441 gtaacttcgg gagaaggggt gctgacttaa agtcagccgc agtgaatagg cccaagcaac
     1501 tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct
     1561 gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga attgaagccc
     1621 cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
     1681 ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag actcggtgaa
     1741 attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc ccatggagct
     1801 ttactgcagt ttgatattga gtgtctgtac cacatgtaca ggataggtag gagtctaaga
     1861 gatcgggacg ccagtttcga aggagacgct gttgggatac tacccttgtg ttatggccac
     1921 tctaacccag ataggtgatc cctatcggag acagtgtctg acgggcagtt tgactggggc
     1981 ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc ctcagaatgg ttggaaatca
     2041 ttcgcagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc gagcagggac
     2101 gaaagtcggg cttagtgatc cggtggttcc gtatggaagg gccatcgctc aacggataaa
     2161 agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg gggaggtttg
     2221 gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt
     2281 tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta
     2341 tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg accagagtgg
     2401 acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct atgtagggaa
     2461 gggataaacg ctgaaagcat ctaagtgtga aacccacctc aagatgagat ttcccatgat
     2521 tatatatcag taagagccct gagagatgat caggtagata ggttagaagt ggaagtgtgg
     2581 cgacacatgt agcggactaa tactaatagc tcgaggactt atccaaagta actgagaata
     2641 tgaaagcgaa cggttttctt aaattgaata gatattcaat tttgagtagg tattactcag
     2701 agttaagtga cgatagccta ggagatacac ctgtacccat gccgaacaca gaagttaagc
     2761 cctagaacgc cggaagtagt tgggggttgc cccctgtgag a
//
LOCUS       DAVKHZ010000122         2796 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000122 DAVKHZ010000000
VERSION     DAVKHZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.122"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(35..496)
                     /locus_tag="VWW99_002010"
     CDS             complement(35..496)
                     /locus_tag="VWW99_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539796.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     DRYLYRPYAGAPRGEKVYGKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(463..564)
                     /locus_tag="VWW99_002011"
     CDS             complement(463..564)
                     /locus_tag="VWW99_002011"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539797.1"
                     /translation="MKGGHRHVEAAKVFDVGVRTLFTWEKKDVNKGT"
     gene            875..1255
                     /locus_tag="VWW99_002012"
     CDS             875..1255
                     /locus_tag="VWW99_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9539798.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1354..1575
                     /locus_tag="VWW99_002013"
     CDS             1354..1575
                     /locus_tag="VWW99_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9539799.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1592..1906
                     /gene="trxA"
                     /locus_tag="VWW99_002014"
     CDS             1592..1906
                     /gene="trxA"
                     /locus_tag="VWW99_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9539800.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2211..2744
                     /gene="queF"
                     /locus_tag="VWW99_002015"
     CDS             2211..2744
                     /gene="queF"
                     /locus_tag="VWW99_002015"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9539801.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT          873 a          593 c          517 g          813 t
ORIGIN      
        1 gtttatattc ctatttttat gcataattat tctttcattc tctaatttgt gaatcaatat
       61 atctgattag aaaactacca ttgagttttt gctccacctc attagtccgc tcaaaacgcc
      121 gtccgctaat cttgccatag actttctccc ctctaggagc ccctgcataa ggacgataga
      181 ggtagcggtc gattcccgtt tcgtcaatat ataggactgg taaatctttt aggttatcca
      241 aaatatcaag aaactcagaa actttctcag gcttttgttc cctaaaactg gtcgtctttt
      301 ttaaaatgac cttaatctgc tttaaaactg cccatacgga gggcaaagca caatcaaaac
      361 gggccgcaat ttcccgtaaa aaagcgtctg gatgagcctc tacaaaggct ttcaattctt
      421 ctaaagggat cttacgcttt ttgacgactc gctttttccg ctctaagttc ccttgtttac
      481 gtctttcttt tcccacgtga agagagttct gacgccgaca tcaaaaactt tggccgcctc
      541 gacatgtctg tgccctcctt taatggaatc taatgctcct tgtttaaaat cagtactata
      601 tgcatagctt tattagacca tgcttatttt aaactaagca actatagata gagaaatgaa
      661 ataaaatctg aattcgaatg tgataaggag ttagaaattg aaacaatagg attcccccat
      721 tattcacaca aaaataaaac cactaaaaaa cagagcttgc cagcattctt gaaaagtagt
      781 ataattattg catgcgcaat catcttatga cgcaaagact gcccgttaat ggactttctt
      841 ctataattat gactctaaaa agaaaggaga cactatgacc tatttggaaa aatggtttga
      901 cttcaatcgg cgtcagaaag aaattgaaag tctcttggaa gagaccattg cccaacagag
      961 cgagcaaagt ctgaccttga aagagttcta cctgctctac tatctggact tggctgaaga
     1021 aaaatctcta cgccagattg atctgccaga taagcttcat ctgagtccaa gcgcagtttc
     1081 taggatggtg gctcgcttgg aagctaaaaa ttgcggtcta ctcagtcgca tgtgttgcca
     1141 tcaagataga cgctctagtt ttatctgcct gacaaatgat gggcaaaaga cactggcctc
     1201 gctacaaaag actgtcgaag aaagcttgga agcaggtttg gatttcttga tttaagatga
     1261 ttttaaaaaa agttgcgtgc gcaatcattt ttcttgacat tctcttttat aaggagtaaa
     1321 ataaagttag cattaaacaa aggagtttta aacatgattg aaattaccta tatagatgcc
     1381 agcaagaacg aaagaactgt gactttcgag tcttatgaag actttgaacg ttcgcaacaa
     1441 gcttgcctta tcggcgtcgc agactactac cctgtccaaa aattaactta caagggtcat
     1501 aatttggact accatgggac ttatggagat atcttcttct atctcatgaa acaagattta
     1561 agccaatata actaaaaaaa ggagaaatac aatggcaaaa gcaattacag atgcaacatt
     1621 cgaacaagaa acaaaagacg gtttggtctt agtagacttc tgggcaactt ggtgtggtcc
     1681 atgtcgtatg caaggtccaa tcttggacaa attgtctgaa gaactttcag aagatgtctt
     1741 gaaaatcgtt aaaatggacg ttgatgaaaa tccaaataca gctcgtgctt ttggaatcat
     1801 gtctattcca actcttctct tcaaaaaaga cggccaagtt gtcaaacaag ttgcaggtgt
     1861 tcacacagca gaacaaatca aggccatcat tgctgaattg agctaatcaa acgagagacc
     1921 aagtactttc tttggtctct tttttcttgc cctttgccat ttttcaaaaa atatgctaga
     1981 ctatagtaga ttgaaactag aatagtacac ctctacttct aaaacattgt tagaaatcga
     2041 tttaactgtc ctgatcgatt tgtcctgttc ttatttcatt ttactatata agtagaatat
     2101 tacgacgttt gggatatgcc atcgctaaaa aaactcgatt tggtattttt tagctcccta
     2161 atgggagctt tttgcgtgct ctgaacattt cccattttgg aaggagtact atgaaacatc
     2221 aatcagcctt ggtcgtcttt agtggtggtc aagattcaac aacctgcctt ttctgggcca
     2281 tgcaacacta tgaaacagtc gaagctgtca cctttgccta cggccaacgt catcacctcg
     2341 aaattcaaat tactagagaa atcgctaagg aacagggcat tcgtcaccat atcctcgata
     2401 tgtctctgct gggacaaatc actgctcagc cagactttgc gacgattcat atttcctaca
     2461 ttcctgacaa gctctgtgtc gagtcaaaat ccctcaaact atatctattt agctaccgaa
     2521 accacggaga tttccacgaa aactgtatca acaccatcgg gaaagacttg gtcaacttgc
     2581 tagaccctcg ctatttagaa gtctggggaa aattcactcc gcgcggtggc atttcaatcg
     2641 acccctacta caactacggt aagcaaggaa ctaagtatga gggcttggca gaacaacgcc
     2701 tcttccaaca cgacctttat ccagagaaaa ttgacaaccg ctaaagtcat actcaatgaa
     2761 aatcaaagag caaactagga agttagccgc aggttg
//
LOCUS       DAVKHZ010000123         2758 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000123 DAVKHZ010000000
VERSION     DAVKHZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.123"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            38..109
                     /locus_tag="VWW99_002016"
     tRNA            38..109
                     /locus_tag="VWW99_002016"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:70..72,aa:Gln,seq:ttg)
     gene            271..1473
                     /gene="rpsA"
                     /locus_tag="VWW99_002017"
     CDS             271..1473
                     /gene="rpsA"
                     /locus_tag="VWW99_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9539802.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1558..2758)
                     /locus_tag="VWW99_002018"
                     /pseudo
     CDS             complement(1558..2758)
                     /locus_tag="VWW99_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          831 a          463 c          619 g          845 t
ORIGIN      
        1 ttcgggggtt cgaatccctc tctctccatt tcattaatgg ggtatagcca agcggtaagg
       61 caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt tcttaggtaa
      121 taatcaagat aaaaagcaaa atatcttagg gtattttatt tttataattg aaagacgtga
      181 atgatatgaa catgtccttg cgggtgctta ggaaaaaaat tataagtatg tcaagtttaa
      241 gaaaaacttg attgttggag gattttttag atgaacgaat ttgaagattt gctaaatagc
      301 gttagtcaag ttgagactgg tgatgttgtt agtgctgaag tattgacagt tgatgcgact
      361 caagctaacg ttgcaatctc tggaactggt gttgaaggtg tcttgactct tcgcgaattg
      421 acaaacgatc gtgatgcaga tatcaatgac tttgttaaag taggagaagt attggatgtt
      481 cttgtacttc gtcaagtagt tggtaaagat actgatacag ttacatacct tgtatctaaa
      541 aaacgccttg aagctcgcaa agcatgggac aaacttgttg gtcgcgaaga agaagttgtt
      601 actgttaaag gaacgcgtgc cgttaaaggt ggactttcag tagaatttga aggtgttcgt
      661 ggatttatcc cagcttcaat gttggatact cgtttcgtac gtaacgctga gcgttttgta
      721 ggtcaagaat ttgatactaa aatcaaagaa gttaacgcta aagaaaaccg cttcatcctt
      781 tcacgtcgtg aagttgttga agcagctact gcagcagctc gcgctgaagt attcggtaaa
      841 ttggctgttg gtgatgttgt aactggtaaa gttgctcgta tcacaagctt cggcgctttc
      901 atcgaccttg gtggtgttga cggattggtt cacttgactg aattgtcaca tgaacgtaac
      961 gtatcaccaa aatcagttgt aactgttggt gaagaaattg aagtgaaaat ccttgatctt
     1021 aacgaagaag aaggacgtgt atcactttca cttaaagcaa cagtaccagg accatgggat
     1081 ggcgttgagc aaaaattggc taaaggtgat gtagtagaag gaacagttaa acgtttgact
     1141 gacttcggtg catttgttga agtattgcca ggtatcgatg gacttgttca cgtatcacaa
     1201 atttcacaca aacggattga aaatccaaaa gaagctctta aagttggtca agaagttcaa
     1261 gttaaagttc ttgaagttaa cgcagatgca gaacgcgtat cactttctat taaagctctt
     1321 gaagaacgtc cagcccaaga agaaggacaa aaagaagaaa aacgtgctgc tcgtccacgt
     1381 cgtccaagac gtcaagaaaa gcgtgatttc gaacttccag aaacacaaac aggattttca
     1441 atggctgatt tgtttggtga tatcgaactt taatcaaatt gaaaattcac aaaatccttt
     1501 gtttactaaa caagggattt ttctggctct ttgtcaactg tagtgggttg aagtcagcta
     1561 aggttgagaa aggacaaatt tcgtcctttc ttttttgatg ttcagagcga taaaaatccg
     1621 ttttttgaag ttttcaaagt tccgaaaacc aaaggcattg cgtttgataa gtttgatgag
     1681 attattggtc gcttccaatt tggcgtttga atagggtagt tgaagggcgt tgacgatttt
     1741 ctctttgttc tttagaaagg ttttaaagac agtctgaaaa agaggatgaa cctgcttaag
     1801 attgtcctca ataagtccga aaaatttctc tggttcctta ttatagagct gatagtggtg
     1861 tttcaagtct tctgaatagc tcaaaagctt gtttaaaatc tctttattgg ttaagtacat
     1921 acgaaaagta gaacgataaa atcgcttatc actcagttta cggctatcct gttgaatgag
     1981 tttccagtag cgcttgatag ccttgtattc atgagacttt cgatgaaact gattcatgat
     2041 ttggacatgc acacgactca tagcacggct aagatgttgt acaatatgaa agcgatcaag
     2101 aacgatttta gcattcggga gtgaaacagt ctgggagact gtttcagcct gagcctagaa
     2161 atttgaaagc gaagttgtct agctaagtca tagtaaggac taaacatatc catagtaatg
     2221 attttcactt gacaacgaac ggctctatca tatttaagaa agtgatctcg gatgatagct
     2281 tgtgttctac cttcaagaac agtgatgata ttgagctttt caaaatcttg cgcaataaag
     2341 ctcatctcca tctcccgatt gaaacagtca ctccccggac tgtttcaacg tcccaagaca
     2401 taatctcagg cagatttcta aaattacact caaagtggaa gtcattgagc tttcgaatga
     2461 cagttgaagt tgagatggaa agctgatggg caatatcggt catagaaatc ttttcaatta
     2521 acttttgagc aatcttttgg ttgatgatac gagggatttg gtgattcttc ttgacgatag
     2581 aagtttcagc gaccatcatt tttgaacagt gatagcactt gaatcgacgc tttctaagga
     2641 gaattctagt aggcatacca gtcgtttcaa ggtaaggaat tttagacggt ttttgaaagt
     2701 catgtttctt catttgactt ccgcactcag ggcaagatgg ggtgtcataa tccagtct
//
LOCUS       DAVKHZ010000124         2741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000124 DAVKHZ010000000
VERSION     DAVKHZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.124"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..339)
                     /locus_tag="VWW99_002019"
     CDS             complement(<1..339)
                     /locus_tag="VWW99_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539803.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCIGDFPHDFF
                     DIYLDHVAKYA"
     gene            complement(397..1110)
                     /locus_tag="VWW99_002020"
     CDS             complement(397..1110)
                     /locus_tag="VWW99_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9539804.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1569..1943)
                     /locus_tag="VWW99_002021"
     CDS             complement(1569..1943)
                     /locus_tag="VWW99_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9539805.1"
                     /translation="MESIGIVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGGIG
                     TSFDQVDRVVSENPADTLLVFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQIQLAELEINK"
     gene            complement(2360..>2741)
                     /locus_tag="VWW99_002022"
     CDS             complement(2360..>2741)
                     /locus_tag="VWW99_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9539806.1"
                     /translation="RNKNGQADTNQTEKPNEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLL
                     FGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          834 a          533 c          434 g          940 t
ORIGIN      
        1 ggcatatttt gctacatgat ctaggtatat atcaaaaaag tcatgaggaa aatctcctat
       61 acagccatta cgattcatat tgaggctata ttttcgatta gtaagataat tcggaaatat
      121 atttcctatt actctaagat tatctacttg tgaatccctt ctcccaatat aatccccttc
      181 tttgactaat gatggagatt tccaagtaaa aattaaatcg ccagagaaat ttttcttatc
      241 aaaaaactta aaaagagatt gataaatact atttgctaat tgacaagtgt cagatgtaaa
      301 ttcataatta acagcttcat taaaatcaac ttcagacatt tcaatgtact cttcaatagt
      361 aatattagct tttttgtagt cattagctcg tctcctttaa aatgtgatct tttcaagaat
      421 atttttatct aaacaatcca gcaagtcttg gtaagaatag acttcgtaag tcggctgggc
      481 ttgtgtgtga ttttcgaggt gatgaggatt ataccagata gtgtcaatcc ccgcattatt
      541 gccaccttga atgtcggcgg ttagagaatc tccaatcatc agcgtctttt ctttactaaa
      601 tccagcaatt tgctggccaa tcttttcata aaaaagagca tccggctttt gagtttgcaa
      661 ctgttctgag ataaagactt gattgaaata aggtgctaga ccagattgag ccaaacgtcc
      721 tgtctgaatg gcagtaatgc catttgtcgc agcatacaag ttataatcac gctcaatgag
      781 gctgtccaag agatcatgag cgcccgatag tgtttgtccc tgctgggcga ggtaaaattg
      841 gtaacgctgg gcaagaaaac taccgtcttt ttcctgtcca aaatgagcaa ataaacgaga
      901 aaagcgcgtg ttaaccagct cttgtttact gattttcttc agctccaagt ctttccagag
      961 agccttgttc ataggaacgt aataatcttt ataagcctga atatccgcaa ctccttcttc
     1021 ttttagaagt tgggtcaaag ccacatcctc agcagcatca aaatcaagaa gagtgtggtc
     1081 gaggtcgaag agtagaaatt tgtagaacaa tttgaggttt tcctttctga aaattcatta
     1141 agaacattat atcataaagc acctcataca attaactaat ttaatcactt aaaaaaaatt
     1201 cgaacacttt ctatacaact gacagctcaa atctttcaga atagaacaat actaactatc
     1261 gagcacccta tattcataaa tatatgtgta attctagacc tagaattcct ataaactaaa
     1321 tgctttcata ctcttccaag taattgattt ccttaaattt taatttttga aggattctaa
     1381 agctagaata gccccatcac aatcagtttt gattgattca caatttagaa gcactatagt
     1441 ttcactcctg ttaaaataaa aaggaattgc ataaagcaat ccctttctga ttttgaaatc
     1501 atttacttaa cattttatag ttgagataat caatagctta cctataaaaa gagttatagt
     1561 aaaattcctt atttattgat ttcaagctcc gctaactgta tttgaataac tgacagttct
     1621 gcaccagcct gaagaagagc agctgcatta taggcacctt ctacaattgg aaccctgttg
     1681 atgatgatac ttttatcact gaaatcagtc accattttta agttcatttt agcagaacct
     1741 aggtcaaaaa atacaagtaa agtatctgct ggattttcgg aaacaaccct atctacttga
     1801 tcaaaactcg ttccaattcc tccgccctcg gttcctccta cataagtaat cggaacatct
     1861 ttagctactt tactaatcag ttcaacaaca ccttctgcaa tgtgtttgga atgtgaaacg
     1921 ataacaatac caatactttc catcaaacca ccccagtttc taaaatagca gtaaagagta
     1981 atcctgatga gaatgatcca ggatcaatat gtccaagaaa ccatatgctc ctaagacaag
     2041 agccaacaga ctggtcatca ataatagtat tgttcttttt ttcatcatta ctccttaact
     2101 agtgtttaac tgattaatta gccagtaaat agtttatctt tatttacact atctgttaag
     2161 atatagtaaa ataagaacag gacagtcaaa tcgatttcta acaatgtttt agaagtagag
     2221 gtatactatt ctagtttcaa tctactatat tttgcacatt ttcatgaaaa aaatgagaac
     2281 tagaactcac attctgctct catttttcgt tttcccgttc tcctatcctg tttttaggag
     2341 ttagaaaatg ctgctacctt tacttactct cctttaataa agccaatagt ttttcagctt
     2401 ctgccataat agtattgttg tcctgggtgc caaatagtaa attatttttt aatcctgtga
     2461 gagtctcttt ggcattggac ttgataattg gatcctggat ttttccaagt aaatcttcag
     2521 cctctctcag tttttcttca accttttcag tctcgacctg aggttcttct gattcctctg
     2581 gtgattcttc ttctggttcc tctgttggtt ttggagactc tggtttctcg ctttgcggtt
     2641 tctcttctcg aggggtttct tcctcaggtt tttctgtctg aggtttctcc tcgtttggtt
     2701 tttccgtttg attggtatca gcttgaccat ttttgtttct t
//
LOCUS       DAVKHZ010000125         2722 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000125 DAVKHZ010000000
VERSION     DAVKHZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.125"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..279
                     /locus_tag="VWW99_002023"
     CDS             <1..279
                     /locus_tag="VWW99_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9539807.1"
                     /translation="LDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHE
                     TLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            373..444
                     /locus_tag="VWW99_002024"
     tRNA            373..444
                     /locus_tag="VWW99_002024"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:406..408,aa:Glu,seq:ttc)
     gene            696..2241
                     /locus_tag="VWW99_002025"
     rRNA            696..2241
                     /locus_tag="VWW99_002025"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2293..2365
                     /locus_tag="VWW99_002026"
     tRNA            2293..2365
                     /locus_tag="VWW99_002026"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2326..2328,aa:Ala,seq:tgc)
     gene            2490..>2722
                     /locus_tag="VWW99_002027"
     rRNA            2490..>2722
                     /locus_tag="VWW99_002027"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          818 a          529 c          767 g          608 t
ORIGIN      
        1 ttagactata tccgtaaaca ggaaagtcag aagcgtagat acgataaaga accctatgaa
       61 gaagtgggtg agatcagtca tcgtataagt gaggggggtc tctggctaga tgattattat
      121 ctctttcatg aaacactaag agattataga aacaaacaaa gtaaagagaa acaagaagaa
      181 ctagaacgcg tcttaagcaa tgaacgattt cgagggcgtc aaagagtatt aagagactta
      241 cgcattgtgt ttaaggagtt tactatccgt acccattagt aagtcatgca aaaaaaatga
      301 aaaaaattag aaaaagtagt tgacaaagtt tgaaaaggct gtataatagt aagagttgaa
      361 aataacaact caggtccgtt ggtcaagggg ttaagacacc gccttttcac ggcggtaaca
      421 cgggttcgaa tcccgtacgg actatggtat gttgcgtcag gagcacttga tgaaaaaaag
      481 tttaaaaaaa cttaaaaatc ttcaaaaaag tgttgacaag cgaaagcagt tgtgatatac
      541 taatatagtt gtcgcttgag agaagcaagt gacaaagacc tttgaaaact gaacaagacg
      601 aaccaatgtg cagggcgcta caacataagt tgtagtactg aacaatgaaa aaacaataaa
      661 tctgtcagtg acagaaatga gtgagaactc aaacttttaa tgagagtttg atcctggctc
      721 aggacgaacg ctggcggcgt gcctaataca tgcaagtaga acgctgaagg aggagcttgc
      781 ttctctggat gagttgcgaa cgggtgagta acgcgtaggt aacctgcctg gtagcggggg
      841 ataactattg gaaacgatag ctaataccgc ataagagtag atgttgcatg acatttgctt
      901 aaaaggtgca cttgcatcac taccagatgg acctgcgttg tattagctag ttggtggggt
      961 aacggctcac caaggcgacg atacatagcc gacctgagag ggtgatcggc cacactggga
     1021 ctgagacacg gcccagactc ctacgggagg cagcagtagg gaatcttcgg caatggacgg
     1081 aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa agctctgttg
     1141 taagagaaga acgagtgtga gagtggaaag ttcacactgt gacggtatct taccagaaag
     1201 ggacggctaa ctacgtgcca gcagccgcgg taatacgtag gtcccgagcg ttgtccggat
     1261 ttattgggcg taaagcgagc gcaggcggtt agataagtct gaagttaaag gctgtggctt
     1321 aaccatagta ggctttggaa actgtttaac ttgagtgcaa gaggggagag tggaattcca
     1381 tgtgtagcgg tgaaatgcgt agatatatgg aggaacaccg gtggcgaaag cggctctctg
     1441 gcttgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt
     1501 agtccacgct gtaaacgatg agtgctaggt gttagaccct ttccggggtt tagtgccgta
     1561 gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat
     1621 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc
     1681 ttaccaggtc ttgacatccc tctgaccgct ctagagatag agttttcctt cgggacagag
     1741 gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc
     1801 aacgagcgca acccctattg ttagttgcca tcattcagtt gggcactcta gcgagactgc
     1861 cggtaataaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg
     1921 gctacacacg tgctacaatg gctggtacaa cgagtcgcaa gccggtgacg gcaagctaat
     1981 ctcttaaagc cagtctcagt tcggattgta ggctgcaact cgcctacatg aagtcggaat
     2041 cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg
     2101 cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaaccgt aaggagccag
     2161 ccgcctaagg tgggatagat gattggggtg aagtcgtaac aaggtagccg tatcggaagg
     2221 tgcggctgga tcacctcctt tctaaggata aggaactgcg cattggtctt gtttagtctt
     2281 gagaggtctt gtggggcctt agctcagctg ggagagcgcc tgctttgcac gcaggaggtc
     2341 agcggttcga tcccgctagg ctccattggt gagagatcac caagtaatgc acattgaaaa
     2401 ttgaatatct atatcaaata gtaacaagaa aataaaccga aaacgctgta gtattaataa
     2461 gagtttatga ctgaaaggtc agaaaataag gttaagttaa taagggcgca cggtggatgc
     2521 cttggcacta ggagccgacg aaggacgtga caaacgacga tatgccttgg gtagctgtaa
     2581 gtaagcgatg atccagggat ttccgaatgg gggaacccaa caggtaatac ctgttaccca
     2641 catctgttaa ggatgtgagg aggaagacgc agtgaactga aacatctaag tagctgcagg
     2701 aagagaaagc aaaagcgatt gc
//
LOCUS       DAVKHZ010000126         2686 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000126 DAVKHZ010000000
VERSION     DAVKHZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.126"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..270
                     /locus_tag="VWW99_002028"
     CDS             <1..270
                     /locus_tag="VWW99_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539808.1"
                     /translation="KILADSGYQGLMKIYSQAQTPRKSSKLKPLTVEDKTYNHALSKE
                     RSKVENIFAKVKTFKMISTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(290..416)
                     /locus_tag="VWW99_002029"
                     /pseudo
     CDS             complement(290..416)
                     /locus_tag="VWW99_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            534..2516
                     /locus_tag="VWW99_002030"
     CDS             534..2516
                     /locus_tag="VWW99_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9539809.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT          901 a          482 c          543 g          760 t
ORIGIN      
        1 aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga tatattctca agcacaaact
       61 ccacgtaaat ccagcaaact caagccgcta acagttgaag ataaaaccta taaccatgcg
      121 ctatctaagg agagaagcaa ggttgagaac atctttgcca aagtaaaaac gtttaaaatg
      181 atttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct
      241 ggtattatca atcatgaact aggattctag ttttgcagga agtctattga aggatctttt
      301 gctttagttc cttggtcaag gttgattatt ttcacggagc gcttccgatt gttttcataa
      361 accatttgcg catagtcagc agaataaatc tttttgaaac gtccctttcc tagacattat
      421 agaactttca gattcatctg aatttaccaa attaacacaa aaattctgaa aattctgttg
      481 acatctttct gaaaagagtt tataatggag agaaagtttt aaaggagaaa atgatgaaaa
      541 gttcaaaact atttgccctt gcgggcgtga cattattggc ggcgactact ttagctgcat
      601 gctctggatc aggttcaagc actaaaggtg agaagacatt ctcatacatt tatgagacag
      661 accctgataa cctcaactat ttgacaactg ctaaggctgc tacagcaaat attaccagta
      721 acgtggttga tggtttgcta gaaaatgatc gctacgggaa ctttgtgccg tctatggctg
      781 aggattggtc tgtatccaag gatggattga cttacactta tactatccgt aaggatgcaa
      841 aatggtatac ttctgaaggt gaagaatacg cggcagtcaa agctcaagac tttgtaacag
      901 gactaaaata tgctgctgat aaaaaatcag atgctcttta ccttgttcaa gaatcaatca
      961 aagggttgga tgcctatgta aaaggggaaa tcaaagattt ctcacaagta ggaattaagg
     1021 ctttggatga tcagacagtt cagtacactt tgaacaaacc agaaagcttc tggaattcta
     1081 agacaaccat gggtgtgctt gcgccagtta atgaagagtt tttgaactca aaaggggatg
     1141 attttgccaa agcgacggat ccaagtagtc tcttgtataa cggtccttat ttgttgaaat
     1201 ccattgtgac aaaatcctct gttgaatttg cgaaaaatcc gaactactgg gataaggaca
     1261 atgtgcatgt tgacaaagtt aaattgtcat tctgggatgg tcaagatacc agcaaacctg
     1321 cagaaaactt taaagatggt agccttacag cagctcgtct ctatccaaca agtgcaagtt
     1381 tcgcagagct tgagaagagt atgaaggaca atattgtcta tactcaacaa gactctatta
     1441 cgtatctagt tggtacaaat attgaccgtc agtcctataa atacacatct aagaccagcg
     1501 acgaacaaaa ggcatcgact aaaaaggctc tcttaaacaa ggatttccgt caggctattg
     1561 catttggatt tgaccgtaca gcctatgcct ctcagttgaa tggacaaact ggagcaagca
     1621 aaatcttacg taatatcttt gttccaccaa catttgttca agcagatggt aaaaactttg
     1681 gcgatatggt caaagagaaa ttggttactt atggggatga atggaaggat gttaatcttg
     1741 cagattctca ggatggtctt tacaatccag aaaaagccaa ggctgaattt gctaaagcta
     1801 aatcagcctt acaagcagaa ggtgtgactt tcccaattca tttggatatg ccagttgacc
     1861 agacagcaac tacaaaagtt cagcgcgtcc aatctatgaa acaatccttg gaagcaactt
     1921 taggagctga taatgtcatt attgatatcc aacaactaca aaaagacgaa gtaaacaata
     1981 ttacatattt tgctgaaaat gctgctggcg aagactggga tttatcagat aatgtcggtt
     2041 ggggtccaga ctttgccgat ccatcaacct accttgatat catcaaacca tctgtaggag
     2101 aaagtactaa aacatattta gggtttgact caggggaaga taatgtagct gctaaaaaag
     2161 taggtctata tgactacgaa aaattggtta ctgaggctgg tgatgaggct acagatgttg
     2221 ctaaacgcta tgataaatac gctgcagccc aagcttggtt gacagatagt gctttgatta
     2281 ttccaactac atctcgtaca gggcgtccaa tcttgtctaa gatggtacca tttacaatac
     2341 catttgcatt gtcaggaaat aaaggtacaa gtgaaccaat cttatataaa tacttggaac
     2401 ttcaagacaa ggcagtcact gtagatgaat accaaaaagc tcaggaaaaa tggatgaaag
     2461 aaaaagaaga gtctaataaa aaggctcaag aagatctcgc aaaacatgtg aaataactgt
     2521 tgcaaaatat aagaaaggat ttagtatttc tcttgaatgc tgaatccttt tttacatttg
     2581 taaagaaaga ttctaaatgt actgaccccc aaaagttgga gcctcttttt gtcagaatag
     2641 agaaaatttt tgttaatttt acttgtttcc tattgctttc tcagct
//
LOCUS       DAVKHZ010000127         2633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000127 DAVKHZ010000000
VERSION     DAVKHZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.127"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..748)
                     /locus_tag="VWW99_002031"
     CDS             complement(<1..748)
                     /locus_tag="VWW99_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539810.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIIKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKE"
     gene            complement(1630..1842)
                     /locus_tag="VWW99_002032"
     CDS             complement(1630..1842)
                     /locus_tag="VWW99_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539811.1"
                     /translation="MLIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            complement(1850..2221)
                     /locus_tag="VWW99_002033"
     CDS             complement(1850..2221)
                     /locus_tag="VWW99_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9539812.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
BASE COUNT          975 a          490 c          320 g          848 t
ORIGIN      
        1 tttctttata aagcaaaggt aaaactttat aaaaaatatc taagtcaacc tcactctgat
       61 tctgaagcaa gtaggagtca agcaaatttt caaaaatagc taacaatatg attggtgtaa
      121 ggtttttgta ttcacatgtc aagtattctg agtatcgtgc agatgatatt tcttttatat
      181 aactttttga aagcaagatt ttttcaaatt catcatctat tattaatctt ctatttccaa
      241 tccaagcggt attaccaccc aaataagatt gtgcaaatag caatatgtaa gaaaatatga
      301 acgtaaatac tgaaagaggt agaattctat gcgtaaaaaa atagacgata atggtagcca
      361 ggagtaaaga tatctctcgt gacaatcgaa ttcttttcag catctttttt aacagtccgt
      421 aaggttgtcc ttggcaatat tcttgaacta aattaatcgg aatgacatct ctcaccattt
      481 ctggaaagta gagtaagttt ataggttgaa atctccagct cccatcataa gtaaatggat
      541 atagaaccaa atacttaaaa gattttttta ttataaactt aattatcaca atatgaatga
      601 ctgttgcaat gaagtatatt aaatataaaa gtaaaaccgc catcaatata cttacaaaaa
      661 tccccttgga ctcaatcaat ggaaaataaa atattgcaac tagaagcgaa taaagtcccg
      721 ctaataaata tttctgatca tctttcattc aaaacaccat attatttact aagaatacat
      781 caatacaata gagcaactct gtaagtcaat atttgataaa gcaaagcttt aaccccttcc
      841 ccagacgtta gcttttcttc aacccactac agttgacaag gagcccaaat tattgataaa
      901 aactttagtc atcatcgtct ctataaaaat attttctata cactcttgat aataccaaaa
      961 ttaataccaa tatcatacca agagctataa taatagaatt tattagattt attttagtat
     1021 atccaatcac tagtccaata attggactag cagacatgct tagatataat aaaaataaca
     1081 aaaaatgaat ttcaccaatt cctgcaaaaa aatgtttcat agctataatt ttacgcccat
     1141 aaatacccat caaatttacc gtctacgata tctaaaccat atgcaatggc tcctcctaat
     1201 gagaagactt cagcaacagt agccgcaata ttaagagcat tagtaatagt tgccatacta
     1261 actccaccaa tatatcttgt tatagcactt agttacctag ataagatatc ctttagcttg
     1321 cttccaaaat ttaagtctgc acctataata tgagttcata ttagtactgc taaatcctat
     1381 ctatagtaat gtcaaacacc taaataaagt catctctccg attcattcaa gaaatgaaga
     1441 aaggcaaaaa atctttaaaa taagaaaagt gcatattatc aggtgttcaa aaatattgat
     1501 gatatacgtt atatagatag aaagatttac tcaatctcac tttcaaattg agttttttcc
     1561 caacctcgtt tttaatttat atcttcaata aaatctaatg acctagttga tatgtcatat
     1621 ggaataatat tatttaatct gtaatttttt taaaaaactt aataataaat ttgctccact
     1681 gattgctatc aatatttcaa ttggtaaaaa atagtaattc caaataaaat gagaaatcag
     1741 caatgataga aaaataacaa tatcaatttt cctgttgttg acatactctg taattttatg
     1801 atacaggaat gcaacaataa ctgaaattaa aacaatcaac ataagattat tatcctattc
     1861 tcaattgatt atcacgtcgc ccatcccagc catcaataat attggtaaaa atccaatcag
     1921 ctccagcacc aattccagca gatgcaatac ttctaataac agtatttgca aaagcatttc
     1981 caatatgaat tcctaatact cctaaacctt taacaatccc agctaccagt gcggagcgta
     2041 atccataaga tgcaagaact agttgaaggc ctccacctcc tgctgctgct aatgctgcat
     2101 atgctgcacc agtaatggct cctgtaattg cccacctggg tatatatata gcccccccat
     2161 caaggtacat catctcttct tcatcaatca caacataatt atttggtaat acaagttcca
     2221 tttattttct ccttttttat taagcatgct taggatggtt tcctaacaag tgatcgcagg
     2281 ctttgtttag ttacctaaat tagacttcct tcctttatct tattttaaat atttaaaacc
     2341 agttactgct tgaagcttaa cagtttactt aattcaaata acagctaatc actgattccc
     2401 tgattttatc aaatatacta ataacaacaa ttgtgatacg atcatcatta taagaacaac
     2461 atttaacgaa tcaaagtttt tccgagataa taataaagca acagcaagca gtattattac
     2521 taaagattga ataactacta ttactcttaa cattttatcc tctactaaca actcaagctc
     2581 ccttacatta tatcgaccag ccccatggaa agattatagc tagactaatc aat
//
LOCUS       DAVKHZ010000128         2537 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000128 DAVKHZ010000000
VERSION     DAVKHZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.128"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            29..652
                     /locus_tag="VWW99_002034"
     CDS             29..652
                     /locus_tag="VWW99_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9539813.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            783..2240
                     /locus_tag="VWW99_002035"
     CDS             783..2240
                     /locus_tag="VWW99_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9539814.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFWLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            2263..>2537
                     /locus_tag="VWW99_002036"
     CDS             2263..>2537
                     /locus_tag="VWW99_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9539815.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQAL"
BASE COUNT          734 a          457 c          551 g          795 t
ORIGIN      
        1 tttttgctag ttagaggagg ttaaactgat gttgaataag ataagagact atctagactt
       61 ttctggtttg cagtaccgta gtcctgataa agcgggagca gagcgagaga agatgctggc
      121 gttccgccac aaaggacaag aggcccgaaa ggcttttaca gaactggcca aagcctttca
      181 agcaagccat ccagaatggc aactccaaca gactagtcag tggatgaatc aggcccagcg
      241 tttgagacca catttttggg tttatctaca gagagacgga caagtgacag aacctatgat
      301 ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag tcagtttcat
      361 cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag acattccaac
      421 cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt gggaggcgaa
      481 tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc gaaaagtttt
      541 agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg tagaaggctt
      601 attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat aagataattt
      661 gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa ttcttggtct
      721 ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa aggagaacaa
      781 ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg cagaatcccg
      841 catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa cctgcccatg
      901 acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac gtgggtgctg
      961 gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa ttccaaattg
     1021 gtgcagcggt tatcgaccct tattttggac ttgctgcagc aaacaacaaa attgtagcag
     1081 agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt ggaataaata
     1141 tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt actggtcaca
     1201 tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta ccacaattgc
     1261 gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg gcagttagtt
     1321 caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt gcgattggtc
     1381 accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt ggtaagaaag
     1441 aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac gatacagttg
     1501 ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc ttgggtccag
     1561 acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct gctaaacaag
     1621 atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg ttcgttttga
     1681 tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt atttcaaaca
     1741 aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga tttggttctc
     1801 caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt acaattgttt
     1861 tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca gtgttctttg
     1921 acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg gctgttatcc
     1981 tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg gcccttctcg
     2041 atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca tggcttggat
     2101 ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt ctcttctggc
     2161 ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat tacaacggtg
     2221 aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa agtattagca
     2281 gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga aaatgctctc
     2341 cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga agctaaaggt
     2401 ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga attggaaggg
     2461 cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga aatcaccgaa
     2521 aaactcagtc aagcact
//
LOCUS       DAVKHZ010000129         2532 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000129 DAVKHZ010000000
VERSION     DAVKHZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.129"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..246)
                     /locus_tag="VWW99_002037"
     CDS             complement(<1..246)
                     /locus_tag="VWW99_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU9539816.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNL"
     gene            complement(449..853)
                     /locus_tag="VWW99_002038"
     CDS             complement(449..853)
                     /locus_tag="VWW99_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9539817.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            complement(850..1014)
                     /locus_tag="VWW99_002039"
     CDS             complement(850..1014)
                     /locus_tag="VWW99_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539818.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            complement(1311..1430)
                     /locus_tag="VWW99_002040"
     CDS             complement(1311..1430)
                     /locus_tag="VWW99_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9539819.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(1534..1683)
                     /gene="blpO"
                     /locus_tag="VWW99_002041"
     CDS             complement(1534..1683)
                     /gene="blpO"
                     /locus_tag="VWW99_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9539820.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(1927..2130)
                     /gene="blpN"
                     /locus_tag="VWW99_002042"
     CDS             complement(1927..2130)
                     /gene="blpN"
                     /locus_tag="VWW99_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9539821.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAEIGGIGGAVVCGYLAYTATS"
     gene            complement(2146..2400)
                     /gene="blpM"
                     /locus_tag="VWW99_002043"
     CDS             complement(2146..2400)
                     /gene="blpM"
                     /locus_tag="VWW99_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9539822.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
BASE COUNT          868 a          522 c          349 g          793 t
ORIGIN      
        1 taagttgctt tcgtggatac caaaatcagc cgcaatttct tcataagtgc ggtattctcg
       61 cacatattga agagtggcca taagaaggtc ttctaggctt aatttaggtt ttcgtccacc
      121 ttttgcgtgt ttaagttgat aagctgtttt taatacagct agcatctctt caaaagtcgt
      181 gcgctgaaca ccaacaagac gcttaaatcg tgcatcagtt agttgtttac ttgcttcata
      241 attcatagaa ctatagtaaa atgaaataag aacaggataa atcgatcagg acagtcaaat
      301 cgatttctaa caatgtttta gaagtagagg cgtactattc tagtttcaat ctactatact
      361 ataccatatt ttgtttcgca gggaatctat tataaaaggg taagtattgc aaaaacactt
      421 acccttttct tttatacttc attaagctct actttttata atacttcaag ccccacatga
      481 gcagaagcat gatgattaag cagagaacag cgccaatata agcgattatt tgttggtagg
      541 attctcctgc tgtgatacct ctatacaaac aaataataga cataaaacct gtcaagccga
      601 tgaacataag ttgattggtt ctaggactaa ccaaatcatc atcttcaaac tctcttatcc
      661 tcatttccct agtgagataa acagtaacca aaatagaagc caagttaata actactaaaa
      721 gaaattggaa aactacggaa aaatttaaaa actgacgaga tagaaataga taagtagaaa
      781 caagcaaggg caactgacct aagaacaatc tcgcaaggaa gatgttccgt tttttagcaa
      841 gaaaagtttt catttctttt ctcctttctt tttattgata gcaaaataga tcataactgc
      901 aatcacatag gctatggtat aaaatagctg ataccaagca ctctccctaa gcggatatag
      961 aaagatggac atgattagat acagaacgaa aataatcagt atttttttct tcataagatt
     1021 tcctcctaaa tgtgcgattt atcttagttg agcaagaaca tttacactgc tagtatagca
     1081 cttattttga ccttggatca ctcaaatcat aaatggtcat caaaacctct tgaattgtaa
     1141 aaattaaaaa agcaagcatg aaaaacatac tttcctcttt atattgtatt gataccaact
     1201 tgtttgtaga cttttcatcc tgctatcaca tatcattttg acaggcgaaa caatattaaa
     1261 gaaactcccc tgtaaattaa gctagcaaat acaggggaga aatttatttt ttagagagta
     1321 ctatccgtat cctttttgga agattttgaa aatatttttc taattaagtc atccatataa
     1381 ggaccaaata taccaactac taaaccaata ataaaacttt taaaatccat aattaccacc
     1441 aacatattgc tgcataggct acacctccaa gtatagctcc acctgcagca ccagttacac
     1501 ctattcctat agcaaatggt cccaatagaa atgtcaaacc gttgttgcac acccatcaat
     1561 tgcgccatat gcaacccctg ctgcacaact aatttttctt ccccaatcaa tatctccacc
     1621 ttcaacgcaa gcaagcattt cattatccat aactgcaaat tgtgacatca tttttgtatc
     1681 catatagtat atcacttttc agttacggaa caagtttaat ataaaaatta tcaaaaaaac
     1741 ataggcaata aagagaaaaa ttaatttatc atagattaga aataatatga caaaacaatt
     1801 caatgatgtt aattcaatag tcttttgttt tttatcggag atacttatgg atagataaat
     1861 aagataggtt tgaaaagcga agagaataat aaagaatata gccttcataa aatttagctt
     1921 tcatttttat gatgtagcgg tataggctaa atatccacaa accactgctc ctccaattcc
     1981 tcctatttca gcgccccatg gtcctagaag tctcccatat ttcactccac ccgctgcaca
     2041 acctaaagca gcaactacag ctgctcctcc ggaattacct ccataaacct cactcagcat
     2101 tgtttcattt atattacaat aagtattcat acaagtctcc ttttattaaa atccacccgt
     2161 tgcccctgtt actcctgccc aaagatccac accaaattta gctcctatgt atccacatgc
     2221 tcccataaat ggtgctccaa caccactcgc agcacaaata gctgtcccta gcccccagcc
     2281 accaaaagca gcaccaccac cttctaagac attagtttgc caattattct tgcctccttc
     2341 aatactagat aacatagtta tatccatttc atgaaattgt tccataattt ttgtatccat
     2401 gacaaatact cttttttatt tttaattttt gtcttgttgt aactttgaca agtttagtat
     2461 atcatcgttt tttaaaattt ttcatccaga tcttgaatag tcatcgaaac gtcttgaatt
     2521 gcaaaaatta ca
//
LOCUS       DAVKHZ010000130         2514 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000130 DAVKHZ010000000
VERSION     DAVKHZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.130"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            31..354
                     /gene="tnpB"
                     /locus_tag="VWW99_002044"
     CDS             31..354
                     /gene="tnpB"
                     /locus_tag="VWW99_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9539823.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(509..817)
                     /locus_tag="VWW99_002045"
                     /pseudo
     CDS             complement(509..817)
                     /locus_tag="VWW99_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            808..1248
                     /locus_tag="VWW99_002046"
     CDS             808..1248
                     /locus_tag="VWW99_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539824.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            1343..1772
                     /locus_tag="VWW99_002047"
                     /pseudo
     CDS             1343..1772
                     /locus_tag="VWW99_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1843..2001
                     /locus_tag="VWW99_002048"
     CDS             1843..2001
                     /locus_tag="VWW99_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9539825.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            2094..2514
                     /locus_tag="VWW99_002049"
                     /pseudo
     CDS             2094..2514
                     /locus_tag="VWW99_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          763 a          474 c          524 g          753 t
ORIGIN      
        1 tagaacagct tttggataag gtgttgctct atgacaattc atctatctag cctaggggag
       61 gtctatctcg tgtgtgggaa aactgatatg agacaaggaa tcgattcact ggcttatctg
      121 gttaaaaccc actttgaatt ggatcctttc tccggtcaag tctttctctt ttgtggtgga
      181 cgtaaagacc gctttaaagt cctttactgg gatggtcaag gattttggct actatataaa
      241 cgctttgaga acggcagatt gatttggcta agtacagaaa aggatgtcaa agctctcaca
      301 ccagaacaag tagactggct tatgaagggc ttttctatca ctccaaaaat atagtagatt
      361 gaaactagaa tagtacacct ctgcttctaa aacattgtta gaaatcgatt ttactgtcct
      421 gatcgatttg tcctgttctt atttcatttt actataaatc catcagaaag tcgtgatttc
      481 tattgaaatg aggactttct ttttatactc atctgctttc aaaaagcatt ctagtccatc
      541 tccgattaac gatggacttt atcacctcct tctccagtcc ttgtataaca tcttggagtt
      601 gattcatgac atcttccaaa gtttaaaagg ctttattctt aaatccacgt ttacgaatct
      661 ctttccacac ttgttcaatg gggttcatct ctggtgtgta tggaggaata aatgcaaagc
      721 caatattagt cggaatcttt aaggtacttg atttatgcca tatagcattg tccataacga
      781 gtaaaagata atcatctgga taagcttgtg aaatctccta ttcctaaagc ccctttagcg
      841 cataactttg gctcagcttc tattatcgct cacaccatcc atcagaagtt taatctgaag
      901 gtacccaatt atcgccaaga agaagattgg gctaggatgg gtttaccaat cacacgtaag
      961 gaaatctcta attggcatat caaggcgagt caatactatt tggagcccct ttataacctc
     1021 ttgcgagaga gactattgac tcagccctta cttcatgcgg atgaaacttc ttatagggtg
     1081 ctagagagtg atagtcagct gacttactat tggacttttt tgtcaggtaa agcagagaaa
     1141 caagggatta cgctttacca ccatgatcag tgtcgaagtg gttcagtagt acaagaattc
     1201 ctaggagatt attctggcta tgtgcattgt gatattttgc ggcagtaact taggacttta
     1261 gtcctctagt tctgcctatg cgatagcagt ccaaggttta ggagcaaggc gacgctaagc
     1321 ttggtaaact tcgaaccgct cgtctgctta tcgtcaactg gaagaagctg aacttgttgg
     1381 atgttgggcg catgtgagaa ggaagttttt tgaagcgccc ccccaagcaa gcggataaat
     1441 catccttagg agctaaaggt ttagcttatt gtgatcagtt attttccttg gaaagagact
     1501 gggaggcttt gccagctgat gaacgactac agaaacgtca agaacatctc cagcccttaa
     1561 tggaagactt ctttgcttag tgccggcgtc agtcagtttt agcaggttca aaactaggaa
     1621 gggcaattga atacagcctc aagtatgaag aaacctttaa gaccattttg aaagacggac
     1681 atctggtcct ttccaataat ctagctgaac gcgccattaa atcattggtt atgggacgga
     1741 gtaaaagagt ccagtggact cttttagcct aagctcagtt taaaaaagcg agggtggtta
     1801 ttttctcaaa gttttgaagg agctaaagca agagctatta ttatgagttt gttggaaaca
     1861 gctaaacgtc atcaattaaa tagcgagaaa tatctatcct atcttctaga atgtcttcca
     1921 aacgaggaaa ctctcgtaaa caaagaggtt ttagaggctt atttaccatg gactaaagtt
     1981 gtacaagaaa agtgcaaata agaaatctcc agattaggaa ctatccgtga gttctccagt
     2041 ctggagattt ttcaatagac ttcctgcgaa acaaaatatg gtataatagt tctatgaatg
     2101 atgaagcaag taaacaacta accgatgcac gatttaagcg tcttgttggt gttcaacgca
     2161 cgacttttga agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag
     2221 gtggacgaaa acctaaatta agtctagaag accttcttat ggccactctt caatatgtgc
     2281 gagaatatcg aacttatgaa caaattgcgg ctgtttttgg tattcacgaa agcaacttaa
     2341 tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggtgttacg atttcaagaa
     2401 ctcctctcag ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc
     2461 ctaaaaaaag aattagcgaa ttattctggt aaaaagaaat ttcacgctat gaag
//
LOCUS       DAVKHZ010000131         2499 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000131 DAVKHZ010000000
VERSION     DAVKHZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.131"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            130..294
                     /locus_tag="VWW99_002050"
     CDS             130..294
                     /locus_tag="VWW99_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9539826.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            378..944
                     /gene="amaP"
                     /locus_tag="VWW99_002051"
     CDS             378..944
                     /gene="amaP"
                     /locus_tag="VWW99_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9539827.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            956..1126
                     /locus_tag="VWW99_002052"
     CDS             956..1126
                     /locus_tag="VWW99_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9539828.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1165..1773
                     /locus_tag="VWW99_002053"
     CDS             1165..1773
                     /locus_tag="VWW99_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9539829.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1804..2007
                     /locus_tag="VWW99_002054"
     CDS             1804..2007
                     /locus_tag="VWW99_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9539830.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2118..2390)
                     /locus_tag="VWW99_002055"
                     /pseudo
     CDS             complement(2118..2390)
                     /locus_tag="VWW99_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          871 a          376 c          482 g          770 t
ORIGIN      
        1 atagtattaa tcaaagaaat tgattttttg atattaaaat aaaaaaggag ggtaggcagt
       61 gttgtgatca attattgctg gaggtcttat tggtctcttg gcaggtaaaa tcactaaaaa
      121 agtagttcta tgggaatcat cgcaaatgta ttcgctggtt tagtcggggc atatgcagga
      181 caatctcttt taggtagttg gggtccatca atcgctggaa tggctttgct cccatctatt
      241 gtaggtgcag cgattgtgat tactgtagtg tcattcttta caggtagaaa gtaaactttt
      301 cgccagtaaa gttagcaaac tatttttaaa tcaatgacgg gaaaaatagt ttaaatgtta
      361 aatcgaaagg attgtatatg tcaaaagcaa agaaaatatg tttcattatt ttctgtattt
      421 taatcttgac aattttcctt cctgttttga tagattatca tcaagttagt gatctaggta
      481 ttcatctact tagctggaga cagaactccg tagttgaatt ctatcttgct agatatgtct
      541 tttgggggac agtggttcta tcaactttag ttttattatc cattttagtt gtgatgtttt
      601 atcctaaacg ttacttggaa atccaacttg aaactaaaaa cgatacatta aaattaaaga
      661 attcggcaat cgaaggtttt gttagaagtt tggtgagtga tcatagattg atcaagaacc
      721 caactgttca tgtaaattta cgaaaaaata aatgtttcgt tcatgtagaa ggtaaaattc
      781 ttccttcaga caacatcgct gacagatgcc aaataattca aaatgaaata actaatggat
      841 tgaagcagtt ttttggtatt gagcgtcaag taaaacttga agttgcagta aaaaattacc
      901 aaccaaaacc tcaaaacaaa aagactgtta gtcgtgtgaa gtaaggaagt aaaaaatgga
      961 atggcttaaa caatatcgat atccaattat cgctggtctc ataggcgtat ttctggcttg
     1021 tttgattgtc tcctttggct tcttcaaaac aatatttgta ttgattttag gagcactggg
     1081 agttgcagct ggattatata tcgaaaaaaa ctatatagat aaataaaaaa ataaaaatta
     1141 ctaatttaat taaaggagtt tcatatgtca aacgaaaaaa acacaaacac taacgtagaa
     1201 aagaaagatg ctactgttgt agctcacgaa atcaaagggg aacttactta cgaagataaa
     1261 gttatccaaa aaatcattgg tctttcacta gaaaacgttt caggtctttt gggaatcgat
     1321 ggtggtttct tctcaaatct taaagaaaaa atcgttaaca gcgatgacgt aacaagtggt
     1381 gttaacgtag aagttggtaa aacacaagtt gcagttgact taaacgttat tgttgagtac
     1441 caaaaaaatg ttccagcttt atattcagaa atcagagaaa tcgtatcttc agaagttgct
     1501 aaaatgactg acttggaaat tgttgaaatc aacgtaaacg ttgtcgacat caaaactaaa
     1561 gaacagcatg aagcagactc agtaagcctt caagatcgcg tatctgacgt tgctgaatca
     1621 acaggagaat tcacttcaga acaattcgaa aaagctaaat ctggtcttgg atctggtttc
     1681 tcaactgttc aagaaaaagt tagcgaaggt gtagaagctg ttaaaggtgc agcaaatggt
     1741 gtagtatctc acgaaaacac tcgtgtaaac taagataaaa taaatataac aggagaaatt
     1801 atcatgtcag tagaagaaaa attaaatcaa gctaaaggtt ctattaaaga aggtgttggg
     1861 aaagccatcg gtgatgaaaa aatggaaaaa gaaggagcag ctgaaaaagt tgtttctaaa
     1921 gtaaaagaag ttgccgaaga cgctaaagac gctgtagaag gtgctgtaga aggtgttaaa
     1981 aacatgttga gtggcgacga taaataaggt taaaagttac tttatctttt ttggtaacat
     2041 tagtcaaaag agtctgagtc aagatgattc tcagaaaaca aaaagctaga gattcccaat
     2101 tgcggaactc tagcttttta attttgcctc tttctcttat tatatttcag caggttgttg
     2161 gccatgagta tgaatcccat gtcaattctc acttgacgct tacctctcag atgacatctc
     2221 ttataaccca aacaaacctt tatctgccca aagacagatt tcatatcaat cttacgttta
     2281 gcgaaaattt gtctaccctt ggaagataaa agtgcctgat attctttagt ttttaaacac
     2341 tggtaacgtt cattcatata cagtcccttt tgaggggctg attcaggttc ataatcgcag
     2401 tcaacattga tttcaaggct gtttgctttc tatctcccct ttttcataat gtatagtaaa
     2461 atgaaataat aacaggacga atcgatcggg acagtcaaa
//
LOCUS       DAVKHZ010000132         2448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000132 DAVKHZ010000000
VERSION     DAVKHZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.132"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            33..484
                     /locus_tag="VWW99_002056"
                     /pseudo
     CDS             33..484
                     /locus_tag="VWW99_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            649..948
                     /locus_tag="VWW99_002057"
     CDS             649..948
                     /locus_tag="VWW99_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9539831.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1209..1496
                     /gene="tnpB"
                     /locus_tag="VWW99_002058"
                     /pseudo
     CDS             1209..1496
                     /gene="tnpB"
                     /locus_tag="VWW99_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1582..2448)
                     /locus_tag="VWW99_002059"
                     /pseudo
     CDS             complement(1582..2448)
                     /locus_tag="VWW99_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          792 a          496 c          438 g          722 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactttc ggagaaaaaa
       61 agtgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggaaaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca agctgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca actcctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatcaaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaatc acgtaagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa atcaagcgac
      601 tctattcaaa aagtgttata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaacttgta aaaagtcaac tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aagacctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttccata acaaaacgca tagtaacaag
     1021 agtttctaca cccactatta tgcgttttta tcattttgaa atctactgct aatcaatgaa
     1081 gaagcaaaga tcaaactagg cagctagtta caagttacgt aagcgcgtca taacaaggta
     1141 tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg gaggaaaggt
     1201 attgtactat gataattcaa ctaagtgatt taggtcaagt tcaccttgtt tgtggtaaga
     1261 cagatatgag gtagggaata gactcattag cctatgtagt taaaacccac tttgaattgg
     1321 attctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc tttaaagccc
     1381 tttactggga tggtcaagga ttttggctac tatataaacg ctttgagaac ggaaaactga
     1441 cttggctaag tgcagaaaag gatgtcaaag ctctcgcacc tgaacaagta gattgactga
     1501 tgaagggctt ttctatcact ccaaaaataa atctatcaga aagtcgtgat ttctattgaa
     1561 atgaggactt tctttttata ctcatctgct ttcaaaaagc attctattcc atctccgatt
     1621 accgatggac tttatcacct ccttctccag tccttgtatg acatcttgaa gttgattcat
     1681 gacatcttcc aaagttcgaa aggctttatt cttaaatcca catttacgaa tctctttcca
     1741 cacttgttca atggggttca tctctggtgt gtatggagga ataaatgcaa agccaatatt
     1801 agtcggaatc tttaagatac ttgatttttg ccatatagca ttgtccataa cgagtaaaag
     1861 ataatcatct ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc actcagtatt
     1921 acatccacca gctattaaga aaaatgattc gcctgtatgg gcatcaacag ctccataaca
     1981 atagcgaaat tctcgtatat agtgactatg gatatgtgga cctactccta ttggagccca
     2041 acaagatccc agtttactga ttctaccgaa accagcctca tcttggtaca tcaagcgaac
     2101 cttacgaaaa cgtctactat atttaaagcg ctttcttatc ttcttgaatt gagattttat
     2161 ttttagacgc aacaatggtt tgagcgtctg ctttcttagg atgttctgga cgtggcataa
     2221 tatttcgcca accatggtgc ttcaacagtt gatagaaggc atcacgtgtg taggaacgac
     2281 ctaactcctt tttataagcc tgaaataagg catcaattgt aacaaattct cctgcctctg
     2341 cagccttcaa atggcgggca agaaaggctt tctcttcctc aactgtcata tatgcatggt
     2401 tacgaccacc acgtgtttct tgaaggagag agtcgagtcc gaactcct
//
LOCUS       DAVKHZ010000133         2443 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000133 DAVKHZ010000000
VERSION     DAVKHZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.133"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..184
                     /locus_tag="VWW99_002060"
     CDS             <1..184
                     /locus_tag="VWW99_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9539832.1"
                     /translation="AQPQLPITKLKKILLPLPPLALQNEFADFVALVDKSQLAIQKSL
                     EELETLKKSLMQEYFG"
     gene            244..480
                     /locus_tag="VWW99_002061"
     CDS             244..480
                     /locus_tag="VWW99_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9539833.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            477..890
                     /locus_tag="VWW99_002062"
     CDS             477..890
                     /locus_tag="VWW99_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9539834.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1008..1973
                     /locus_tag="VWW99_002063"
     CDS             1008..1973
                     /locus_tag="VWW99_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9539835.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1939..>2443
                     /locus_tag="VWW99_002064"
     CDS             1939..>2443
                     /locus_tag="VWW99_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9539836.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSS
                     GKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWIS
                     DNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQ
                     NEFADFVA"
BASE COUNT          881 a          382 c          461 g          719 t
ORIGIN      
        1 tgctcagcct cagttaccaa ttacaaaatt aaaaaaaata cttctccccc ttcccccact
       61 agccctccaa aatgagttcg cagactttgt agccctggtc gacaaatcac aattggcaat
      121 ccaaaaatct ctggaagaac ttgaaacttt gaagaaatct ctgatgcagg agtattttgg
      181 ctgatattct gccattgtaa ttacggtaat gatttgttat aatacttcaa aggaggaaat
      241 cagatggtag taaaaacaag aaaacaagga aattcaatca ccattacgat tccaagtgaa
      301 tttaatattc caagtggtgt taaatacgaa gcgaaattgt taccaagtgg tgagattatc
      361 tttactcctg aagaattggg gcagcaggtt tcttatgtat ctgatgatgc ctttgactta
      421 aatttagata aaatatttga cgaatacgac gatgttttca aagctttggt ggaaaaatga
      481 caatctatct gacagaaaag caaattgaaa aaataaatgc tttagcaatt caacggtatt
      541 ctccaaatga gaaaattcaa acagttagtt cttctgcctt aaatatgatt gtgaacttac
      601 cagaacaatt tgtctttggg aagtctcttt atccaacaat ttttgataaa gcaacgatac
      661 tatttgtcca attgataaag aagcatgttt ttgctaatgc taataaaaga actgctttct
      721 tcgttttggt caaattttta caattaaacg gctatcgttt ttctgtaacg gtagaagaag
      781 cagtaaaaat gtgtgtaacc atcgcagtag aagctttaac tgatgaaaaa atgacaagct
      841 actccaaatg ggtttctgaa cattctgtta gagaaaaggt caaaaagtaa cctagtatgc
      901 tggatttgaa tgagcacaag aaaataaatg aacagacaat attagaattc tgtaatgcag
      961 aaactgatat tgtctctttt tattgatgaa taagaaagtg agaaattatg gaatcaaaag
     1021 ttacaattat catgcaagaa atgttacctc ttttaaataa tgaacaatta ctagcgttga
     1081 gagagagttt agaacatcat ctagtagacg gaaaaaagca gcagaagtat tcgaataata
     1141 acctgttgca actatttatt accgccaagc aggtagaggg ctgtagctca aaaacaattc
     1201 gttattacca gaggacgatt gaaaacttgt ttaatgctat taaagagtct gtgacacaac
     1261 tcacaacaga tgatttaagg agttatttag caaattacca gtctgaaaag gattgtagta
     1321 aggcaaattt agacaatatt aggcgtatat tgtcttcttt ttttgcttgg cttgagcaag
     1381 aggaatatat cattaaaaat cccattcgac ggataaagaa aattaagact gagcaaaatg
     1441 tgaaggaaac ttatactgat gaacatttgg aaattatgcg tgataactgt gaaaatttga
     1501 gagatttggc aataatagac ctactagcat cgacaggtat gcgtgtaggg gagcttgtac
     1561 agttgaatcg ttcagatatt gattttgaaa acagagagtg tgttgtcttt ggtaaaggaa
     1621 agaaggagag accagtatat tttgatgctc ggacgaaaat tcatttaaga aattatctta
     1681 acgacagaaa agatagtcac cctgctcttt ttgtaacgct agttggaaaa gcccagagac
     1741 ttggaattgc tggtgtagag attcgcttaa gaaagttagg agacaaactc ggcatacaaa
     1801 aggttcaccc acataagttc agaagaactt tagcgactaa ggcaattgat aaaggtatgc
     1861 ctatcgaaca agtccaaaaa ctgctaggtc atagcaagat tgacacaacc ctggcctatg
     1921 ccatggtcaa tcaaaataat gtcaagcatt cacaccaaaa attcatctct taaaagcaaa
     1981 tcccgattta acgagatgtt tggggatcct ttaaataata acaagaaatt tgcagtaaaa
     2041 acgggacaac agtgttttaa attttcaagt ggaaaatttc ttgataaaca tgatagagtt
     2101 tttgaaggtt atcctgccta tggtggaaat ggcattgctt ggaaaagtcg aaaatattta
     2161 attgataatc ctactatcat cattggtcgt gtaggtgcat attgtggtaa tgtaagaact
     2221 acccacggta aggtatggat aagtgacaat gctatatata taaaagaatt taagaattcc
     2281 gatttcaatc ttgtattttt attagaatta atgaaagtaa ttgattttag taaatttgct
     2341 gatttctcag gtcaaccaaa gataactcaa aaaccattag aaaatcagaa atatatcctc
     2401 cccccactag ccctccaaaa tgagttcgca gactttgtag ccc
//
LOCUS       DAVKHZ010000134         2392 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000134 DAVKHZ010000000
VERSION     DAVKHZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.134"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..375)
                     /locus_tag="VWW99_002065"
     CDS             complement(<1..375)
                     /locus_tag="VWW99_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539837.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKHDFQKPSKIPYLETTGMPSRILLRKRRFKCYHSSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAH"
     gene            complement(514..1038)
                     /locus_tag="VWW99_002066"
     CDS             complement(514..1038)
                     /locus_tag="VWW99_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9539838.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1091..1327)
                     /locus_tag="VWW99_002067"
     CDS             complement(1091..1327)
                     /locus_tag="VWW99_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9539839.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(1395..2315)
                     /locus_tag="VWW99_002068"
     CDS             complement(1395..2315)
                     /locus_tag="VWW99_002068"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9539840.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
BASE COUNT          686 a          424 c          467 g          815 t
ORIGIN      
        1 atgggcaata tcagtcatag aaattttttc aattaacttt tgagcaattt tttggttgat
       61 gatacgaggg atttggtgat ttttctttac caggggagtc tcagcaacca tcatttttga
      121 agagtgatag cacttgaaac ggcgttttct aaggagaatt ctagaaggca taccagttgt
      181 ttcgaggtaa gggatcttag acggtttttg aaagtcatgt ttcttcatta gacttccaca
      241 atcagggcaa gatggagcct cataatccag cttagcgata atttctttgt gggtatccat
      301 attgatgata tctagaatct tgatgtttgg gtctttaata tcgagcagtt ttgtgataaa
      361 atgtaattgt tccatatgaa tctttctaat gatggttttg tcgcttttca ttataggtca
      421 tatgggactt tttttctaca ataaaatagg ctccataata tctatagtgg atttacccac
      481 tacaaatatt atagagccca aaaaggaagc cctttatgaa ttgtaggact tccttttctt
      541 atccagaaat tgatctagct ctctctgatt tcgaagaata gtgactttat gtgaatattc
      601 ttggcaaagt ttttggtaat tttctttttg agttttgcta cgcccatccc aaagaatcca
      661 tctgataaac tcccactcaa agcgttcagg gcaatctacc gccatacttt ctctgacttt
      721 tccacggtat ttaagataac gcttaaaggc tctaaagaga caggtcaatg gcgaaaaatt
      781 gagaaagatg atttggtcag cttcttgcat tcgttcttgg tagtagcacc aagaataatt
      841 accatcgatg acccaagctt tatgcttggt gagaaagttt tttatctcgg ttaacatcca
      901 ttcgcagtca ctgtcttgcc aaccaggttg aaattggagt gtgtccatgt gcagttttgg
      961 aatggagtag tagttagata acttttctgc tatagttgac ttaccagaac cagaatatcc
     1021 gataattgcg attttcattt tctacctttt cctatttgga gacaaaaaaa cagcctctat
     1081 ggactgtttc ttatttaaca agtttagctg aaagacgagc tttatcgcgg cttgctttgt
     1141 ttttgtgaat caaaccttta gtttctgctt tatcgatagc tgagctagca gcacggaaaa
     1201 gttcttcaga tgggtttgct tcgaaagctt ttatagcagt acgcatagct gatttttgag
     1261 ctgagttctt ttcgttttgt ttaacgttca attcagcgcg tttgatagct gatttaatgt
     1321 ttgccaatgt tcttacctcc atatttacta actataccat tatatatgaa aacttacgtt
     1381 ttgacaaggg aaaattactt ttttaagtaa atttcatcga tttcatggtt cttgctttta
     1441 tgaagaatca cttctgcacg atttctggtt ggttcaatat aattttgcag atttgtgaga
     1501 ttgatactga tccagacctg atgggcaaag gactccactt ccccaatcgg catctgagta
     1561 aaacgataat agtagctatc agggtcgttt tgggctagac tcagcatttt caagaaacgg
     1621 tccagatacc aactttcaat atcatccact ccagcatcta catagatgga aaagtcaaag
     1681 aagtcagtga tatagagacg atcgttttgt ggattttgaa agacattgat tccctcaaca
     1741 attacaaaat cagcagcttt gacactttgt ttctcttcgg gtacgatgtc gtaaacttca
     1801 tgagaataga caggaatatc tacatcttgt ccatttttga tgcggtccaa gaagttgaga
     1861 agagcttcca tatcatagct ttcaggaaat cctttacgat ttaaaatccc ctgctcaatc
     1921 aaggtttgat tgggatagag aaaaccatca gttgtaacca actcaaccgt agcatctgta
     1981 aacgtacggg acagtaggat ttgaagtagg cgactggttg tggattttcc aacggcaaca
     2041 ctcccagaaa ccccaataat aaaaggttga gatttacttt cacgttggag gaaaattcct
     2101 tttgaaaagg ctaaatcttc cttagttcgc ttgtaaatct gaatcaaatg agccaagggg
     2161 agatagatat ctgtaacgtc ttggagactg atttggtcat taaaactctt gatagattcc
     2221 aattcttctt ctgtcaaagg aggtgttgtc tttcgatgta aagattgcca agtctggcgg
     2281 ctgatttttt caaaatgtaa aaattcgttg gtcatttgtt ttcccctaga tattttgtct
     2341 atcataccat aaaaagctaa aaaaataaag cggtttcttg aatgtggtaa gt
//
LOCUS       DAVKHZ010000135         2382 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000135 DAVKHZ010000000
VERSION     DAVKHZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.135"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..415)
                     /locus_tag="VWW99_002069"
     CDS             complement(<1..415)
                     /locus_tag="VWW99_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU9539841.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
     gene            537..1283
                     /locus_tag="VWW99_002070"
     CDS             537..1283
                     /locus_tag="VWW99_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9539842.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNMATLENAGIMVKVVS"
     gene            1296..2276
                     /locus_tag="VWW99_002071"
     CDS             1296..2276
                     /locus_tag="VWW99_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9539843.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKESSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          854 a          292 c          454 g          782 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccggac
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
      481 aggttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg tgtaaaatga
      541 aaagattgga acaaattatt aaattagtat cagaatatga aaagatcgac gttaatacat
      601 tatcggaaaa attaaatgta tcgaaagtaa cgattagaaa agatttagat aaattagagt
      661 caaaaggttt attacacaga gagcatggat atgctgtatt aaatagtgga gatgacttaa
      721 atgtacgttt gtcaattaac tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat
      781 tggtgtcaga taatgaaaca ataatgatag aatctggatc gacctgtgct ttacttgctg
      841 aggaaatttg caagcaaaaa agaaatgtta cgattgtaac aaattcattt tttatagcaa
      901 attttgtgag agcttatgat tcatgtcgta ttattgttct tggtggagag tttcagaagg
      961 attcacaggt tactgtggga cctttattaa aagaaatgat acagactttt catgtgcgtc
     1021 aagcttttgt tgggacagat ggctacgata aagagatggg ctttacagga aaagatttaa
     1081 tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga taaagtcatt gtgctaactg
     1141 actcaagtaa atttgataaa aggggtacag taagaagatt tgctttaagt caagtctatg
     1201 aagtaataac agacgaaaaa ctttctaaac aaaatatggc tacattagaa aatgctggga
     1261 taatggttaa ggtagtttcg taagaggtta agtgtatgaa tcaagatagg aataaactgc
     1321 tctctaaaat tgcttatctg tattatattg aaaacttaaa tcagtcacaa atagcagcaa
     1381 aattaggaat ttatagaacc tctattagta gaatgttaac agaagcaagg aatgcaggaa
     1441 ttgttaaaat tgaaatagag aattttgata ccaatatgtt taagttggaa aattatgtaa
     1501 aagaaaaata tggtctggaa agtttagaaa ttattccaaa tgaatttgat gataatccaa
     1561 caattttatc tgaaagaatt tctcaagttg cagcaggtgt ccttaggaat ctaattgatg
     1621 ataatatgaa aattggcttt tcttggggga aaagtttaag taatttagta gatttaattc
     1681 acagtaaaag tgtccgaaat gttcacttct atcctctagc aggtggtcct agtcacatac
     1741 acgctaaata ccatgtgaat acactgattt atgaaatgtc tagaaaattt catggagagt
     1801 gtacatttat gaatgcaacg attgtgcaag aaaataaatt gttagcagat ggtattttgc
     1861 aatcaagata ttttgaaaat ttgaaaaata gttggaaaga tttagatata gctgtagtcg
     1921 gaattggtga ttttagcaat aaaggaaagc atcaatggtt agacatgctt acagaggatg
     1981 actttaaaga attgaccaaa gttaaaactg taggagaaat ttgttgtcga ttttttgatt
     2041 caaaaggtaa agaagtctat gaaaacttac aggaaagaac gatagcaatc tctttggaag
     2101 atttaaaaaa tattcctcaa agtttagctg ttgcttacgg tgatacgaaa gaatcttcga
     2161 ttctttctgt cttgcgtgct aatttagtaa atcatttgat tacagacaaa aatacaattt
     2221 taaaagtttt ggaagaagat ggggatttga cttttagaga gattctaggt gagtgaaaat
     2281 gatagactga ttcagtttat cgtttttctt tttagttgat tgcacatttg tgcttatata
     2341 aacaaaaata gtttatctgt tgtttttgga ttgacaactt ta
//
LOCUS       DAVKHZ010000136         2382 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000136 DAVKHZ010000000
VERSION     DAVKHZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.136"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..812
                     /locus_tag="VWW99_002072"
     CDS             <1..812
                     /locus_tag="VWW99_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9539844.1"
                     /translation="IYFYSKSKDFKFNTIFTEYSSTTNIDQILVERKRDGNSKTIYKV
                     DNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIKIATDKNDI
                     VLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIEAYR
                     TKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNECLNESISLLQNA
                     INSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKSQQLL"
     gene            complement(1327..2319)
                     /locus_tag="VWW99_002073"
     CDS             complement(1327..2319)
                     /locus_tag="VWW99_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539845.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          873 a          367 c          315 g          827 t
ORIGIN      
        1 acatttactt ttattcaaag tcaaaagatt ttaaatttaa tacaattttt acagagtatt
       61 cttctactac aaatatcgac caaatactag tggaacgaaa acgagatgga aactctaaaa
      121 ctatatataa ggttgataat aatggtaact atattctagc aaaagagaaa aatggagttc
      181 ccctttcaga tgtttggaat ataccatttc ttaatccaaa agctaaagaa agagtaggtt
      241 atcctacaca aaaacctatt ctgttattag aacaaattat aaagattgct actgataaaa
      301 atgatatagt tttagacccg ttctgtggaa gtggaactac tttagtagcc tccaagattt
      361 tgaatagaaa ttatatgggg attgatttat ctgaggaagc tatcaatata actcagcaac
      421 gtctggaaaa tgttataaaa acaagttcaa atttattgaa taaaggaatc gaagcatata
      481 gaaccaaaac tgaggaagag gaaaacattc ttaaattatt acaggcaaaa attgttcaaa
      541 gaaataaagg aattgatggt tttttaccta aacattttca aaaaaaaccg atacctataa
      601 aaattcaaaa aaataatgaa tgtctgaatg agagtatctc tttattacag aatgctataa
      661 actccaaaaa acttgatttt ggagtagtta taaaaactca ttcagataat ttattatttg
      721 attttgatac gattcctgaa aatataatag tagttgacca ttttgaactt acaatcgaaa
      781 aatggttaag taaaagtcaa cagttactat agtttgcaaa ttatttacag aaagaacaaa
      841 aatgctctga aacactgatt tcaaagcatt tttttgttag tttaaaatta ctcccattct
      901 tcttttccaa acgtacaata tatccaaaac cattcaaaat actagattct attttttata
      961 atatcactaa atccacctaa ttataggacg ttttcagatt tttagtcccg tcccagtccc
     1021 agtaccggag aaatattgtt ttaatataat atctcttttt gtcttctaag ctcttaaaag
     1081 caaaagaaca agtaaagagt caagacaagg ataaaaagtc catattaggg caaataaaaa
     1141 gctttaagac agatgacaaa tctaagtcaa ataagaaaga ccatagcaaa ggtgcagaga
     1201 gataaatatt ggcggtcttc ggactgcctt tattttttta tccatttttc aaatcaaatt
     1261 tattcagact atatatgcac atatacactt aaattcatat aaaaacatgg cttgtaaaaa
     1321 attactttaa tcacaataat cgcatttaaa attgtgatgt ttgcaagcta aattacggac
     1381 ttcacttgga agttttccct tgtatctttt ataatagata gaaaatttgc tggcagatga
     1441 atatccaaca gattctgcta tctcttttat aggtagttca gtgtttaaaa gaagagtttc
     1501 agctacattc attctttttc tttgagtgta ctctgtaatg ctttgacaat atttttcctt
     1561 aaataaattt tttaatttag taccactcat tttagatatt ttttcaagcg tgccttgatt
     1621 tacattcgtt gcaaaatgat catctaagaa tcttgctaca tcttcaagtg ctttatcatc
     1681 atcaatttca attttatatt tttttctatt taagtatgtg tcaattacta tacttatcca
     1741 ttcatttgcc tttgctttaa agaaaaaatc agcggcagga gcgtccatct tacaatttaa
     1801 tatttccatt gccactcttt ctaaggcctt tgtaagtatt atttgattcg gttgaagcaa
     1861 ggttgaataa aaagattctg gattaatgtt aatagatgct aaatgttttt ctattagctc
     1921 ttttttaaaa ccaatggaaa cagcaagata acaacaattc tcgtgtaata aaaaaacaaa
     1981 atcatctttt atattatcaa aatcaaaagt acatagagag tttgcggtaa tagtttgata
     2041 cggattaaac ttttctccgt ttgcactgac aatgtaactt gaataaattg aaacatagtc
     2101 tgacatacta taagtgctat tttgaactac ttcctctttg atataaaaat catgtatatc
     2161 gataatgaag ctgcctcctt cataaaacca atataggcct tctgcataag ttgaattctc
     2221 ttttttccaa caaaatgtat gacctgcact tgaatacttc tcattgtttt tacattcatc
     2281 tactttcata taatctttta caaaatcata atatgacata acacactatc ccttttagac
     2341 aatattccaa ttagccttat taattcaaaa ctattgtatt ag
//
LOCUS       DAVKHZ010000137         2240 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000137 DAVKHZ010000000
VERSION     DAVKHZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.137"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..454
                     /locus_tag="VWW99_002074"
     CDS             <1..454
                     /locus_tag="VWW99_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEU9539846.1"
                     /translation="LSENSKKFISKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLL
                     ILPATANVIGKIANGIADDLVTATVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKEL
                     GHIFLFESKKTYEVGLRKAIDSTCSMLQPQSLVKELIKLENIVLEEGH"
     gene            676..1782
                     /locus_tag="VWW99_002075"
     CDS             676..1782
                     /locus_tag="VWW99_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU9539847.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHHNDFMREVYLWTKNLTY"
     gene            1758..>2240
                     /locus_tag="VWW99_002076"
     CDS             1758..>2240
                     /locus_tag="VWW99_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539848.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     K"
BASE COUNT          821 a          271 c          383 g          765 t
ORIGIN      
        1 actttctgaa aattctaaaa aatttataag taaggaatta attagttatt tttgtgataa
       61 agtttatgat gaaatatttg ttgaagaggt agttccgcac gtttttctgc catatgaatc
      121 tgacttactt cttattttac cagctacggc aaatgtgatt ggcaaaattg ctaatggtat
      181 tgctgatgat ttagttacag caactgtttt aaactttaat aaaaaaataa ttttttgtcc
      241 caatatgaac tctactatgt gggacaatca catagttcaa agaaatgtat caattctaaa
      301 ggagttggga catatatttt tatttgagtc taaaaaaaca tatgaggtag gattgcgtaa
      361 agcaatagat tcaacatgtt caatgttaca accacagtcg ttagtaaaag aacttatcaa
      421 attagaaaat attgtccttg aagagggaca ttaaaaacta ctgagaatat taaggagggg
      481 aaaaaaatgg aaaattcatc aatcgatgta gatatgctgt tggaagaatt gacacaagaa
      541 gcaatggtcg ttgttgctgt tgataaggac tgttaattta aacttatggc aatatatgaa
      601 aggttactgg atgttttaaa ttatgcaggc agtagccttt tattatatac aaatggataa
      661 agtaaggata atacaatgat taataaaaaa atacaacaag ttgttttgga atcattacag
      721 aattttttga atgggaactt catttcgcct tgtgtagtct atgattttgg cttgctggaa
      781 actgtacttg atgaatttaa aaatcaaatt cctgtaacat tcaattacca acttttttat
      841 gccgttaaag caaattcaaa tgagaagata cttgaattct tagtagataa aattgatgga
      901 gttgatgtgg cgtcattatc tgaattagat gtggctaaaa aatttttccc accaactcaa
      961 atttctgtta atggtcccgc attttcttat gaaactttat ataatctgat taaaaaacaa
     1021 tataaagttg atattaactt tttggaacat cttcaacaat tttccccaaa agaatctgtt
     1081 ggaataagag taacggagcc agatgaactt aataatcgta tgagtcgatt tggaataaat
     1141 atttgcagtg ataattggac tagtaattta caaaatcctt taattacacg actgcatttt
     1201 cattttggag aaaaagatga taaatttatt gttaagttag ataaaatatt atttaagtta
     1261 caagaaatta ataaacttag agaggttaga gaaataaatc ttggaggcgg ttttatgaaa
     1321 ttatttatgg aaaatcgttt gaaagaattt tttctatcac ttatggaaat ctataaaaag
     1381 tacgatattg atagtactgt gactacaata atagaaccag gtagtgcaat tacttcattt
     1441 tctgcctata tgattactag cccagttaat gttagtgagg tgaatgagca gcaggttatc
     1501 acgttagaca catcaatata caccaataca ttatggtttg ttccgcatat tattacaacg
     1561 ttaaattcaa gtagtaaaga gcgttatagt actattctct atggtaatac ctgttatgaa
     1621 catgacaagt ataaaatgaa agtttcgctt ccaaggttaa ctcaaaatag cagtatagtg
     1681 ttttttcctg taggagctta tataaaaagc aatcattcaa atttacatca taatgatttt
     1741 atgcgggagg tatatttgtg gacaaaaaac ttgacatatt agataaagtt aaggaatatt
     1801 taggaaataa aactactcaa attctggata atcaatataa agaatttttg aaacttaatg
     1861 atataaggcg agcgtttggt atttcagaaa aagtattaaa caattctttt aattttacga
     1921 gtaaagaatt taatgattta attaataacg aaaattattt attcgaatat gcatgtagaa
     1981 ttagagagga atggagaaaa aaatgcttta atcattctta tcgttttcta tgctcaccta
     2041 taattacaga tgattttctt aacacgaaga cattgagaag tagccaaatt gaatataaat
     2101 atgagcgata tttatcgaaa agttcgatag gcgatagagc ggttgatggc tttgtttcct
     2161 tcaatacttt aacagctaat ggtatgtctg ctattaaact atgtcttgag atattaaact
     2221 ctattttctt caagaagaag
//
LOCUS       DAVKHZ010000138         2168 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000138 DAVKHZ010000000
VERSION     DAVKHZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.138"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            43..915
                     /locus_tag="VWW99_002077"
     CDS             43..915
                     /locus_tag="VWW99_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9539849.1"
                     /translation="MIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQT
                     MLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKD
                     GFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLI
                     VSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPI
                     ACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSP
                     YLIYLLIKMNRRNI"
     gene            915..1709
                     /locus_tag="VWW99_002078"
     CDS             915..1709
                     /locus_tag="VWW99_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539850.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          802 a          332 c          358 g          676 t
ORIGIN      
        1 ttcattttac ttatcggaga tgaaagttat tctttttcaa ctttgattaa agtcttaaat
       61 agtgaaactg ttcctggagc tagtttttcg attatggaaa ttagattacc aaaattatta
      121 gcaggaatta tagctggctg gtcttttgga ttggcaggat ttatctttca aactatgtta
      181 agaaatcctc ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc tattgcagcg
      241 gtcttttgca tattggtatt aaaaacaaat agtttaacta ctggaattat ttcaataact
      301 tgtggactaa catcatcttt aatattattt ttactagcta aaaaagatgg tttttcagca
      361 gcaagactga taatattagg tattggtttt caagctgtca caagagcagg cacctcattt
      421 ttattgttga aagtagcaag atatgaatta caagaagtta tgagatggct cagtggctct
      481 ttatctttta caaagttaga tgacatacct cttgttctaa tagtaagtat tattgctact
      541 atattagttt tattttttaa taaaagacta gaaattattg aacttggtga agaaatagca
      601 atcggacttg gagcaaatcc cgagctttca aggcttgttt taattttttg cgctgtatct
      661 ttaactgctt tttctacttc aattacagga ccaatagctt gtatatcttt tttagctggt
      721 cccatagcct taaatattgg caagaaaaga agcccaatat tagctggatt ggttggaatt
      781 ttactagttt tgttatcaga catattctct caaaatattt taccagctag atatccagta
      841 ggtgttgtaa ctggcttgtt aggttcacca tacttaatat acttactaat aaaaatgaac
      901 aggaggaata tataatgaaa ggtctgtggt caaataatct tacctgcggt tatgatgaaa
      961 aaataatctt ggaaaatata aatataaaaa tacctgaaga aaaaatatca gttattattg
     1021 ggtcaaatgg ttgtgggaaa tcaacactca ttaaaacctt gtctcgactt ataaagccat
     1081 tagagggaga agtattgctt gataataaat caattaattc ttataaagaa aaagatttag
     1141 caaaacacat agctatatta cctcaatctc caataatccc tgaatcaata acagtagctg
     1201 atcttgtaag ccgtggtcgt ttcccctaca gaaagccttt taagagtctt ggaaaagatg
     1261 accttgaaat aataaacaga tcaatggtta aggccaatgt tgaagatcta gcaaataacc
     1321 tagttgaaga actttctggg ggtcaaaggc aaagagtatg gatagctcta gccctagccc
     1381 aagatacaag tatcctactt ttagatgagc caactactta cttggatatc tcatatcaaa
     1441 tagaactatt agacctcttg actgatctaa accaaaaata taagacaacc atttgcatga
     1501 ttttgcacga tataaatcta acagcaagat acgctgatta cctatttgca attaaagaag
     1561 gtaaacttgt tgcagaggga aagcctgaag atatactaaa tgataaacta gttaaagata
     1621 tctttaatct tgaagcaaaa attatacgtg accctatttc caattcgcct ctaatgattc
     1681 ctattggcaa gcaccatgtt agctcttaat aattgaatat cggaattcat ttaaaagtcg
     1741 caaattttga actgaccctc aaaagttagg ccaaaaaact aactaaagga ggtcagtttt
     1801 ttaatggcaa atacagcaca gaatttaaga taaaattagt aaaagaatat catgaatgaa
     1861 aaataggata tagagaataa cagcaatatt aaaaagatca ggactgataa taaacaaaaa
     1921 gaaagtacaa cgactagttc aaaagctaaa acttcaagta aaaagttatt caagaaaatc
     1981 tagaaaatac tcatcctaca aaggacaggt aggaaaaata tcagacaaca aaataaaaag
     2041 aaatatttat taccgaaaaa aagaggaaat cgagtcaagt accaacaggc tcatctattc
     2101 caaaaagata tttactcgat ggaaaaactc ctagaattag tgctacaaat attgacaatg
     2161 gtatctta
//
LOCUS       DAVKHZ010000139         2064 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000139 DAVKHZ010000000
VERSION     DAVKHZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.139"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..998
                     /locus_tag="VWW99_002079"
     CDS             <1..998
                     /locus_tag="VWW99_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9539851.1"
                     /translation="LAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKK
                     PERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDD
                     LDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDS
                     KALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSK
                     DPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQ
                     KDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            988..1995
                     /locus_tag="VWW99_002080"
     CDS             988..1995
                     /locus_tag="VWW99_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9539852.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
BASE COUNT          729 a          293 c          365 g          677 t
ORIGIN      
        1 ttttagctat gtgtatagtt tttagcgctt gttcttctaa ttctgttaaa aatgaagaaa
       61 atacttctaa agagcatgcg cctgataaaa tagttttaga tcatgctttc ggtcaaacta
      121 tattagataa aaaacctgaa agagttgcaa ctattgcttg gggaaatcat gatgtagcat
      181 tagctttagg aatagttcct gttggatttt caaaagcaaa ttacggtgta agtgctgata
      241 aaggagtttt accatggaca gaagaaaaaa tcaaagaact aaatggtaaa gctaacctat
      301 ttgacgattt ggatggactt aactttgaag caatatcaaa ttctaaacca gatgttatct
      361 tagcaggtta ttctggtata actaaagaag attatgacac tctatcaaaa attgctcctg
      421 tagcagcata caaatctaaa ccttggcaaa ctttatggag agatatgatt aaaattgatt
      481 caaaagcctt aggtatggaa aaagaaggtg atgagttaat caaaaatact gaagctcgta
      541 tatccaaaga attagaaaaa catccagaaa tcaaaggaaa aatcaaagga aaaaaagtat
      601 tatttactat gattaatgct gcagatacat caaaattctg gatttatact agcaaagatc
      661 caagagcaaa ttatttaaca gatttaggtc tagttttccc tgaatcatta aaagaatttg
      721 agagtgaaga tagttttgca aaggaaattt ctgcagaaga agcaaataag ataaatgatg
      781 ctgatgtaat cataacttat ggtgatgata aaactcttga agctttacaa aaagatcctc
      841 ttttaggtaa aataaatgca attaaaaatg gtgccgttgc tgtaattcca gataatacac
      901 cgttagcagc ctcatgcact ccaacaccac tttcaataaa ctatactatt gaagaatatc
      961 taaatctttt aggaaatgca tgcaaaaatg cgaaataaaa aacaaataaa cctaggcata
     1021 atttttgtaa tctgcctagg tcttcttatt acaatatttt tgtcattaaa gcttggaaca
     1081 aaagaaatta atatcagaga ttttttagca gcttttggaa tgggtaatac aaatgatgat
     1141 tttattaaat caattatata taaaagaata cctagaacta tttttgcaat tttagcaggt
     1201 tctagtcttg ccataagcgg tgtattgatg caatcagtta ctagaaaccc aatagctgat
     1261 ccaggtatac tcggtataaa cacaggagca agtcttagtg tagtaattgg tctttctttt
     1321 ttaggaattt catcaagcat aagccatata agttttgcaa tcattggtgg cttagtaagt
     1381 gcaatttttg tatacgcgat tgctgtaagc ggaaaagcag gccttacccc tataaaactt
     1441 gccttatcag gaacttgtgt tagtatggct ttaagcagtt ttgtaagttt tttaatttta
     1501 ccgaataata acgtcttaga caaatttaga ttttggcaaa taggtagcct tggagcagct
     1561 acattatctt ctatatctac actactacct tttataattt taggtcactt gatagctata
     1621 tttatttcat cagatttaaa cgctttagct atgggtgatg aaatggctgt tggtcttgga
     1681 gttaatgtta ataggataag atcacttgca ataattgcaa gtgtgctttt atgttcaagt
     1741 attactgcaa ttggtggacc tattggcttc gtaggtctta tagttcctca cttttgtggc
     1801 ttatttataa gcaaagatat acgcacaatg accatttctt catcttttat aggtgcagag
     1861 ctcttgctta tatgtgatat aatcggccgt atgttaggta aaccaggtga aattgaagta
     1921 gggataatta ctgcaataat cgggggtcca gtacttattt atgtaactat gaaaaataga
     1981 ggggttaata actaatgcaa aatttaatta taggtattca aaaaagaaaa aatagaataa
     2041 cactattttc ctcactattt ttat
//
LOCUS       DAVKHZ010000140         1947 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000140 DAVKHZ010000000
VERSION     DAVKHZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.140"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            9..1601
                     /locus_tag="VWW99_002081"
     CDS             9..1601
                     /locus_tag="VWW99_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539853.1"
                     /translation="MKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMNLERFLKLS
                     IYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLS
                     WLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKI
                     FASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQ
                     RKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGE
                     LGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEI
                     LKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMV
                     SNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDD
                     GRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVT
                     HAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          685 a          237 c          356 g          669 t
ORIGIN      
        1 aatcaaatat aaaaaaattt agtttattaa cgatatttat tgttgctggt caattattgc
       61 taatttatgc agcaactatt aatgctctgg tgttgaatga attaattgcg atgaatttag
      121 agcggttttt gaaattgtca atctaccaaa tgattgtctg gtgtgggata atattccttg
      181 actgggtagt gaaaaattat caggttgaag tgatccaaga gtttaatcta gagattcgaa
      241 atagagttgc cacagacatc tctaactcta cctatcaaga atttcatagt aaatcatcag
      301 gaacatatct ttcgtggcta aataatgatg ttcagacttt aaatgatcag gcttttaaac
      361 aacttttttt agtaataaaa ggaatttctg gtactatatt tgcagttgtg actcttaatc
      421 actatcattg gtcattgact gtagccacct tgttttcatt aatgattatg ctacttgtac
      481 caaaaatctt tgcatcgaaa atgcgagaag ttagtctaaa tttaactaac caaaatgaag
      541 cttttttaaa atctagtgag actatattga atggatttga tgtgttagcg tccttgaatc
      601 ttttatatgt attgcctaag aaaattaaag aagcaggaat tttattaaag atggttatac
      661 aaagaaagac aactgtagaa acgttagcag gcgctattag cttctttctc aatatttttt
      721 ttcagatatc tctcgttttt ttaacaggct atcttgcaat aaaaggaata gtgaaaattg
      781 gtactattga agcaatagga gcactaacag gtgttatttt tacagcgcta ggtgaattag
      841 gaggtcaatt atcctctatt attggtacga agcctatttt tttaaaattg tattcaatta
      901 atccaattga gtcaaataaa atgaatgata tcgaaccaaa tgaggtgaat agagattttc
      961 cgttatatga agcaaaaaat atttgctata agtatggaga taaagaaata ttaaaaaact
     1021 taaatttttg ttttcaacgt aatgaaaagt atttaatttt aggtgaaagt ggaagcggga
     1081 aatctacatt attaaaatta ttgaatggct ttttgagaga ttatagtgga gaattgcgat
     1141 tctgcgggga tgatataaaa aaaacctcct atttaaatat ggtttcgaat gttctatatg
     1201 tagatcaaaa agcttatttg tttgaaggta cgattagaga taatatttta ttggaagaaa
     1261 attatactga tgaagaaata ctacagtctt tagagcaagt tggtttgagt gtaaaagatt
     1321 ttcctaataa cattttagat tattatgttg gtgatgatgg gagattactg tcaggagggc
     1381 agaaacaaaa aattacttta gctagaggcc taattagaaa taagaaaata gtattaattg
     1441 acgagggaac ttctgctatc gataggagaa cttcgttagc gattgaacgt aagatattag
     1501 atagagagga tttgactgtc attattgtta cccatgctcc gcatccggaa cttaaacaat
     1561 attttactaa gatatatcaa tttccaaagg attttattta agaatatagg ggagggaaac
     1621 atgagattct attgtgaaac ttatgaggta attactgaag agaaaaaagt atcatttttt
     1681 tatgtagatg agaaaccctt aaggtatctg ttatttgact atatatttct actagttcta
     1741 gctaaaaaaa atatcccaat cttatgaact gcaccccaaa agttagattt attctgtcta
     1801 acttttgggg tgcagttcat tttgcatagt gttggcattc atcccagata tccatataaa
     1861 atgtctgaga ttgttgaaat gtctaaaaaa atatcagatc taaacgataa aaaagaattt
     1921 cacaaaaaga actgtgagtt gaggttt
//
LOCUS       DAVKHZ010000141         1923 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000141 DAVKHZ010000000
VERSION     DAVKHZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.141"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(146..687)
                     /locus_tag="VWW99_002082"
                     /pseudo
     CDS             complement(146..687)
                     /locus_tag="VWW99_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(763..1074)
                     /locus_tag="VWW99_002083"
     CDS             complement(763..1074)
                     /locus_tag="VWW99_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539854.1"
                     /translation="MGLPITRKEISNWHIKASQYYLESLYNLLREKLLEQALLHADET
                     SYRVLESDSQLTYYWTFLSGKAEKQGITLYHHDQRRSGSVVQEFLGNYSGYMHCDMLR
                     Q"
     gene            1194..1501
                     /locus_tag="VWW99_002084"
                     /pseudo
     CDS             1194..1501
                     /locus_tag="VWW99_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1656..>1923)
                     /gene="tnpB"
                     /locus_tag="VWW99_002085"
     CDS             complement(1656..>1923)
                     /gene="tnpB"
                     /locus_tag="VWW99_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9539855.1"
                     /translation="MRQGIDSLAYLVKTHFELNSFSGQVFLFCGGRKDRFKVLYWDGQ
                     GFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFSITPKI"
BASE COUNT          602 a          388 c          367 g          566 t
ORIGIN      
        1 gaagacattc tagaagatag gatagatatt tctcgctatt tataatggat tgaaataaga
       61 tatgaacaaa tcgattagga aaggaaaatt gatttataga aatattttag cagtcgatgc
      121 gtactgttat agattcaacg cactataatt gatgacgttt agctgtttcc aacaaactca
      181 taataatagc tcttgcttta gctccttcaa aactttgaga aaataaccac cctcgctttt
      241 ttaaactgag ctcaggctaa aagagtccac tggactcttt tactccgtcc cataaccaat
      301 gatttaatgg cgcgttcagc tagattattg gaaaggacca gatgtccgtc ttttaaaatg
      361 gtcttaaagg tttctttata cttgaggctg tattcaattg cccttcctag ttttgaacct
      421 gctaaaactg actgacggcg gcaccaagca aagaagtctt ccattagggg ctggacgttt
      481 ctgtagtcgt tcatcagctg gcaaagcctc ccagtctctt tccaaggcaa ataactggtc
      541 acgataggct aaacctttag ctcctaagga tgatttatct gcttgcttgg gggtcgcttc
      601 aaaaaatttc cttctcacat gcgcccaaca tccaacaagt tcagcttctt ccagttgacg
      661 ataagcttct agcggttcgc agtttaccaa gcttagcgtc gccttgctcc taaaccttag
      721 actgctatcg cataggcaga actagaggac taaagttcta agttactgcc gcaacatatc
      781 acaatgcata tagccagaat aatttcctag gaattcttgc actaccgaac cactccgacg
      841 ctgatcatgg tggtacagcg taatcccttg tttctcagct ttcccagaca aaaaagtcca
      901 atagtaagtc agctgactat cactttctaa gacccgataa gaggtttcat ctgcatgaag
      961 aagagcttgt tctaacaact tttctcgtaa aaggttataa agggactcta aatagtattg
     1021 actcgccttg atatgccaat tagaaatttc cttacgtgtg attggtaaac ccatcttagc
     1081 ccaatcttct tcttagcgat aattaggtac cttcagatta aacttctgat ggatggtgtg
     1141 agcgataata gaagctgagc caaggctatg cgccaaaggg gctttaggaa taggagcttt
     1201 cacaagctta tccagatgat tatcttttac tcgttatgaa caatgctata tggcataaat
     1261 caagtacctt aaagattccg actaatattg gctttgcatt tattcctcca tacacaccag
     1321 agatgaaccc attgaacaag tggggaaaga gattcgtaaa cgtggattta agaataaagc
     1381 ctttcaaact ttggaagatg tcatgaatca acttcaagat gttatacaag gactggagaa
     1441 ggaggtgata aagtccatcg ttaatcggag atggactaga atgctttttg aaagcagatg
     1501 agtataaaaa gaaagtcctc atttcaatag aaatcacgac tttctgatgg atttatagta
     1561 aaatgaaata ataacaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt
     1621 tagaagcaaa ggtgtactat tctagtttca atctactata tttttggagt gatagaaaag
     1681 cctttcatca gccaatctac ttgttcaggt gcgagagctt tgacatcctt ttctgtactg
     1741 ggccaagtca gtctgccgtt ctcaaagcgt ttatataata gccaaaatcc ttgaccatcc
     1801 cagtaaagga ctttaaagcg gtctttacgt ccaccacaaa agagaaaaac ttgacctgag
     1861 aaagaattca attcaaagtg ggttttaacc agataagcca gtgaatcgat tccttgtctc
     1921 ata
//
LOCUS       DAVKHZ010000142         1844 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000142 DAVKHZ010000000
VERSION     DAVKHZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.142"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            28..>1844
                     /gene="iga"
                     /locus_tag="VWW99_002086"
     CDS             28..>1844
                     /gene="iga"
                     /locus_tag="VWW99_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HEU9539856.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYHEETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPKYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQGAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPENNDSKNENSEKTGEEVPVNPNEGTVEGTS"
BASE COUNT          717 a          306 c          370 g          451 t
ORIGIN      
        1 atataaattt tatttcaagg aggaataatg gaaaagtatt ttggtgaaaa acaagagcgt
       61 ttttcattta gaaaattatc agtaggactt gtatctgcaa cgatttcaag tttatttttt
      121 atgtctgtat tagctagttc atctgtggat gctcaagaaa ctgcgggagt tcactataaa
      181 tatgtggcag attcagagct atcatcagaa gaaaagaagc agcttgtcta tgatattccg
      241 acatacgtgg agaatgatga tgaaacttat tatcttgttt ataagttaaa ttctcaaaat
      301 caactggcgg aattaccaaa tactggaagc aagaatgaga ggcaagccct agttgctggt
      361 gctagcttag ctgctctggg aattttaatt tttgctgttt ccaagaaaaa ggttaagaat
      421 aaaacggtat tacatttagt attggttgcg ggaatgggaa atggtgtctt agtttcagtc
      481 catgctttag aaaatcatct tttgctaaat tacaatacgg actatgaatt gacctctgga
      541 gaaaaattac ctcttcctaa agagatttca ggttacactt atattggata tatcaaagag
      601 ggaaaaacga cttctgattt tgaagtaagt aatcaagaaa aatcagcagc cactcctaca
      661 aaacaacaaa aggtggatta taatgttaca ccaaattttg tagaccatcc atcaacagta
      721 caagctattc aggaacaaac acctgtttct tcaactaagc cgacagaagt tcaagtagtt
      781 gaaaaacctt tctctactga attaatcaat ccaagaaaag aagagaaaca atcttcagat
      841 tctcaagaac aattagccga acataagaat ctagaaacga agaaagagga gaagatttct
      901 ccaaaagaaa agactggggt aaatacatta aatccacagg atgaagtttt atcaggtcaa
      961 ttgaacaaac ctgaactctt atatcatgag gaaactatag agacaaaaat agattttcaa
     1021 gaagaaattc aagaaaatcc tgatttagct gaaggaactg taagagtaaa acaagaaggt
     1081 aaattaggta agaaagttga aatcgtcaga atattctctg taaacaagga agaagtttcg
     1141 cgagaaattg tttcaacttc aacgactgcg cctagtccaa gaatagtcga aaaaggtact
     1201 aaaaaaactc aagttataaa ggaacaacct gagactggtg tagaacataa ggacgtacag
     1261 tctggagcta ttgttgaacc cgcaattcag cctgagttgc ccgaagctgt agtaagtgat
     1321 aaaggcgaac cagaagttca accgacatta cccgaagcag ttgtgaccga caaaggtgag
     1381 actgaggttc aaccagagtc gccagatact gtggtaagtg ataaaggtga accagagcag
     1441 gtagccccat tgccaaaata taagggtaat attgagcaag taaaacctga aactccggtt
     1501 gagaagacca aagaacaagg tccagaaaaa actgaagaag ttccagtaaa accaacagaa
     1561 gaaacaccag taaatccaaa tgaaggtact acagaaggaa cctcaattca aggagcagaa
     1621 aatccagttc aacctgcaga agaatcaaca acgaattcag agaaagtatc accagataca
     1681 tctagcgaaa atactgggga agtgtccagt aatcctagtg attcgacaac ctcagttgga
     1741 gaatcaaata aaccagaaaa taatgactct aaaaatgaaa attcagaaaa aactggagaa
     1801 gaagttccag taaatccaaa tgaaggcaca gtagaaggta cctc
//
LOCUS       DAVKHZ010000143         1812 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000143 DAVKHZ010000000
VERSION     DAVKHZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.143"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            19..936
                     /gene="adcAII"
                     /locus_tag="VWW99_002087"
     CDS             19..936
                     /gene="adcAII"
                     /locus_tag="VWW99_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9539857.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLDRVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            944..>1812
                     /gene="phtB"
                     /locus_tag="VWW99_002088"
     CDS             944..>1812
                     /gene="phtB"
                     /locus_tag="VWW99_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9539858.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     NQAGQKAENLTPDEVSKREGISAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHV"
BASE COUNT          607 a          332 c          395 g          478 t
ORIGIN      
        1 atggttaagg agttgttcat gaagaaacaa aatttatttt tagtcctgtt aagtgtcttt
       61 cttttgtgct tgggggcttg tggtcaaaag gaaagtcaga caggaaaggg gatgaaaatt
      121 gtgaccagtt tttatcctat ctacgctatg gttaaggaag tatctggtga cttgaatgat
      181 gttcggatga ttcagtcaag tagtgggatt cactcctttg aaccttcggc aaatgacatc
      241 gcggccatct atgatgcaga tgtctttgtt taccattctc atacgctaga atcttgggca
      301 ggaagtttag atcccaatct aaaaaaatct aaagtgaagg ttttagaggc ttcagaggga
      361 atgactttag accgcgtccc aggactagag gatgtggaag caggggatgg agttgatgaa
      421 aaaacgctct atgaccctca cacttggcta gatcctgaaa aagctggaga agaagcccaa
      481 attatcgctg ataaactttc agaggtggat agtgagcata aagagactta tcaaaaaaat
      541 gcgcaagcct ttatcaaaaa agctcaggaa ttgactaaga aattccaacc aaaatttgaa
      601 aaagcgactc agaaaacatt tgtaacacaa catacagcct tttcttatct agcgaagaga
      661 tttgggctta atcaacttgg tattgcaggt atctctcctg aacaagaacc aagtccacga
      721 caactaacag aaattcagga atttgttaag acctataagg ttaaaacgat ttttacagaa
      781 agtaacgctt cttcaaaagt agctgaaact cttgtcaaat caacaggtgt gggtcttaaa
      841 actctgaatc ctttagagtc agacccacaa aatgacaaga cctatttaga aaatcttgaa
      901 gaaaatatga gtattctagc agaagaatta aagtgaggaa agaatgaaaa tcaataaaaa
      961 atatctagca ggttcagtgg cagtccttgc cctaagtgtt tgttcctatg aacttggtcg
     1021 tcaccaagct ggtcaggtta agaaagagtc taatcgagtt tcttatatag atggtaatca
     1081 ggctggtcaa aaggcagaaa atttgacacc agatgaagtc agtaagaggg aggggatcag
     1141 cgccgaacaa atcgtcatca agattacgga tcaaggttat gtgacctctc atggagacca
     1201 ttatcattac tataatggca aggtccctta tgatgccatc atcagtgaag agctcctcat
     1261 gaaagatccg aattatcagt tgaaggattc agacattgtc aatgaaatca agggtggtta
     1321 tgtcattaag gtaaacggta aatactatgt ttaccttaag gatgcggccc atgcggacaa
     1381 tattcggaca aaagaagaga ttaaacgtca gaagcaggaa cgcagtcata atcacaactc
     1441 aagagcagat aatgctgttg ctgcagccag agcccaagga cgttatacaa cggatgatgg
     1501 gtatatcttc aatgcatctg atatcattga agatacgggc gatgcctata tcgttcctca
     1561 cggcgaccat taccattaca ttcctaagaa tgagttatca gctagcgagt tagctgctgc
     1621 agaagcctat tggaatggga agcagggatc tcgtccttct tcaagttcta gttataatgc
     1681 aaatacagct caaccaagat tgtcagagaa ccacaatctg actgtcactc caacttatca
     1741 tcaaaatcaa ggggaaaaca tttcaagcct tttacgtgaa ttgtatgcta aacccttatc
     1801 agaacgccat gt
//
LOCUS       DAVKHZ010000144         1811 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000144 DAVKHZ010000000
VERSION     DAVKHZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.144"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..581)
                     /locus_tag="VWW99_002089"
     CDS             complement(<1..581)
                     /locus_tag="VWW99_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9539859.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFAIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVY"
     gene            complement(578..1060)
                     /locus_tag="VWW99_002090"
     CDS             complement(578..1060)
                     /locus_tag="VWW99_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU9539860.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKDRGIKNILIYIKSNLKWYIISYLLLILAALIEGIKL
                     "
BASE COUNT          720 a          292 c          210 g          589 t
ORIGIN      
        1 tcataaaccc gtccttcctc tattaaaatc ctttgagatt tgctaatcaa ttcttgattc
       61 aataaattat gagatacata tataatagta gagctttctt tcatggaaat taagcgttta
      121 gcaatcatat caatacttct tgcatccact gttgataaag tctcatcaat aaaaatataa
      181 tcccaatttt tagcaaaaat taaggataag cctaatttga attttgttcc agtagatgct
      241 gtcatatagt tatctccgat tttattatca aaaaaagtat taatttgttc tttatcctca
      301 aatgatagat ggatttgaaa agaatcacac aacaactgca aattttcttc tatagcaaat
      361 ggaacaaccc caagctgatt ggtatcaagg tatagtacat ctttggagac acaattcttt
      421 aaaccactat caaaagtctc tagcccagct aaaagttgta gaagggtgct ctttcctgaa
      481 ccattctctc cttcaactct gtaaattccc ttatcaatct ctagtgatat atttttcaat
      541 atacatttat cataactttt agattcattt tcaattttca taatttaatt ccctctatca
      601 acgctgctaa aattaataaa agataagaaa tgatatacca ttttaaatta ctttttatat
      661 aaataagtat atttttaatt cccctatcct tcaataatcg gtataaaaga tactgactca
      721 taccttgata ccaaataaaa ttcggtattt caataagagc atgaggtaat attcctttta
      781 ttgtagctac tattccaatc atctgaccag atatacccaa ttgtattcct aaagatagca
      841 aatcaaaaag ttgcaataag ggcgaaatcg gaaaacagaa tagatatagc aaaaaatttg
      901 tagaatttgc ctgtaaaact gtcaatgtaa attgtaattc cagtttcgga ggtatagtag
      961 caaaagtctc attttttaat ataaagctta aaagtaaacc tagtaaaaaa cagactatac
     1021 aaaataacac atagtaaatc agaaaaccac tttttctcat ctaatgcccc ctactaaaat
     1081 tacaaatatt atcagaaaac aatataaaac cagaaaactc catctcaata aactaactgt
     1141 acctttaatt aaaaatagag ctttcttata atagacatag gctataaaat aattgaatgg
     1201 aatcaataac aaattcaaca aaatactttt tatatcatta ctataacttg tcaccggaat
     1261 caaacataac aacaatgaaa attgtaagaa agagattaaa tgcgtcaaca tcattagttg
     1321 tgcattctta aaaaagaaat tcattttaga atgattaaga gcagataatt gaagtaaagc
     1381 tagatctacc ccaattgagg atttatgatt ccctattatt gcctccatta atgtaatata
     1441 tattgaggta taaagaaata atattggtat ttttgtgtca tcaattgata acatcgtcat
     1501 catataccaa aatagatagg aaagaagaat actaatcaaa ataaaagaaa aagttaaccc
     1561 tttatttaac caacaaaaac gaagcaatat aaatagactg ctacgaaatc ttttttgact
     1621 cattcgactg cttttaaaat agaaattact cgcatgatta tctaatatcc ccatgactaa
     1681 aataaaataa ttgagtagta tgacataaat tacaacttgt ccttgttcta tagacaatag
     1741 tcctatagat aaaaataaga acaatttact tataattgaa aacaaactag gtagaattaa
     1801 agaaataatt t
//
LOCUS       DAVKHZ010000145         1798 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000145 DAVKHZ010000000
VERSION     DAVKHZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.145"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            370..846
                     /locus_tag="VWW99_002091"
     CDS             370..846
                     /locus_tag="VWW99_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539861.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            846..1607
                     /locus_tag="VWW99_002092"
     CDS             846..1607
                     /locus_tag="VWW99_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HEU9539862.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          663 a          240 c          347 g          548 t
ORIGIN      
        1 atatcagtaa aaacaacact ttattgaggt attggatacg ctttgctaat agcctaataa
       61 tcacatgtgg agtgttgcta caacgaaaaa ggtgataatc cttgatttca agctatttta
      121 taagcatttt gtctttgtag ataaaggcaa ttttgacaat aaaaatccta aaaggtgaat
      181 cgttatagat gtatttgtag atatcgtttg cgcatcgaaa aaattaatac aagaataaat
      241 atttatagct ctttaggtga cttttataga agtaaagttt aggatagaaa aacaagaaat
      301 aacgcaccat ttttggtgcg ttatgctttt ttatgctata atggatttat aaaaataaag
      361 gagtttgcta tgattggaaa gaacataaaa tccttacgta aaacacatga cttaacacaa
      421 cccgaatttg cacgaattat aggaatttct cgaaatagct tgagtcgtta tgaaaatgga
      481 actagttcag tctctacgga attaattgac atcatttgtc agaagtttaa tgtatcttat
      541 gtcgatattg taggagaaga taaaatgctc aatcctgttg aagattatga attgactttg
      601 aaaattgaaa ttgtgaaaga aagaggtgct aatctattat ctcgactcta tcgttatcaa
      661 gatagtcagg gaattagcat tgatgatgaa tctaatcctt ggattttaat gagtgatgat
      721 ctatctgact tgattcatac gaatatctat ttagtagaaa cttttgatga aatagagaga
      781 tatagtggct atttggatgg aattgaacgt atgttagaga tatctgaaaa gcggatggta
      841 gcttaatgga aatccaagat tatactgata gtgaattcaa acatgcttta gcaaggaatc
      901 ttcgttcact gacaagagga aaaaagtcca gtaagcaacc tatagcgatt ttgcttggag
      961 ggcaaagtgg tgccggtaag actacaattc atcgtattaa acagaaagaa tttcaaggaa
     1021 atattgttat catagatggt gatagttttc gttctcagca tccacactat ttagaactgc
     1081 agcaagaata tggcaaagac agtgtagaat ataccaaaga ttttgcagga aaaatggtag
     1141 agtctttagt aacaaaattg agtagtttga gatacaatct tttgatagag ggaactttac
     1201 gaacagttga tgttccaaag aaaacagcac aactcttgaa aaataaggga tatgaagtac
     1261 aattggcctt aattgcgaca aagcctgaat tgtcgtatct aagtactctt atccgttatg
     1321 aagaactgta cattatcaat ccaaatcaag cacgcgcaac tccaaaagaa catcatgatt
     1381 tcattgtaaa tcatctagtt gataacacac gaaaattgga agaactagct atctttgaaa
     1441 gaattcaaat ttaccaacga gatagaagtt gtgtatatga ttcaaaagaa aatacaactt
     1501 cagcagcaga tgttcttcaa gagttactct ttggggagtg gagtcaggta gagaaggaga
     1561 tgttgcaggt gggggaaaag agacttaatg aattacttga aaaataaaca attgatattt
     1621 ttaggagaat agaaatgaga gggtttaata acaagataaa gtctgtttat caagaactaa
     1681 caaattccaa agagaaattc ggtagctttc acaagacttt aattcatttg catacacctg
     1741 tttcttatga ttacaagcta ttttctaatt ggactgcaac gaaatataga aaaattac
//
LOCUS       DAVKHZ010000146         1646 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000146 DAVKHZ010000000
VERSION     DAVKHZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.146"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            534..1120
                     /locus_tag="VWW99_002093"
                     /pseudo
     CDS             534..1120
                     /locus_tag="VWW99_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1133..1603
                     /locus_tag="VWW99_002094"
     CDS             1133..1603
                     /locus_tag="VWW99_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539863.1"
                     /translation="MVRVYKSVRLAYEAKVWIDELIQKKERKIKELNQDNFLNTLEQT
                     LLSNHYDELNGVSFNIVLKASIGSIIEEAYRNTKHYSIAKWQNLRQKMEKDIKTVNPS
                     LETSVTPRLYLDENVLNGLDEFRYHLMKEDGATRLPRLSYIIKLVVYSYWKTQQ"
BASE COUNT          637 a          232 c          253 g          524 t
ORIGIN      
        1 gtacaactga tgaaaagatt taaaattata acaacgcata atatttcggt aaaacttgat
       61 attgtgcgtt ttatcatgga catttttcta ctcaaattga gatttatgtt tttccacatc
      121 agtctttaat taatttttta atacttaact taacaagtgg gaaacaacga aacaatagac
      181 agcttaaaaa attggagttt ttttaaaaaa actcttgcaa ttctttttta aagtgttata
      241 ataatttgta taagttgtcc taatacaaca atcaaaaaaa caggaggatt cttatgaaac
      301 atcaagaagt tattgattgg ttgaaatatt gtgatgaaaa taatatcaaa ccatgggaat
      361 gggattttaa aaaccactca ctttctccac tacccaatga tagtattact gcttttttaa
      421 aatacagaca gctccctgaa aatgagggta tagattgtga taaagctgca ttctttctat
      481 ataaatgttt gggatggcaa gaatcacaag atagtaccat tagaggagaa acaatgaact
      541 cctttattac catctttaag gaggccattg gaaatagttc aaattttgat gaagagtcaa
      601 caaaattagg cataacaaag aagaaaatcc caatttctgt tctttgtgaa catcagaatt
      661 atttaaaatt tgaaactatt caaaataatt tagagaactt taaattattt gcaagaacaa
      721 ctcatacaat cggaaatttt acagtatttc ctaattggat gaattgtggt agaggtctta
      781 gactaggtga ttactgggat atcactttaa tatcacttca agaattttta aacattctat
      841 ctcctgaagc atgggaaaac ttcataaaaa tgtaccacct tcaaccatat gttaattctg
      901 attatagtgt agagttattt tgggataatc ataataataa tgccatcagg cccacaaaag
      961 aagagaattc tcaattttcc tcaaaaaagt caatgagcga attgaagaac gtggtaaatt
     1021 tatcattaaa caaatctgtg ataagttaga tcaaaaagat ttcaatttct ataaggaaat
     1081 taaaaatatg gagacaataa gattttctaa tgaattttaa aaaggaggat ttgtggtcag
     1141 agtatataaa agtgttcgtc ttgcatacga agccaaagta tggatagatg agcttattca
     1201 aaaaaaagag cgtaaaatca aagaactaaa tcaggataat tttctaaata ccctagaaca
     1261 aacacttcta tcaaatcatt acgatgaatt aaatggtgtt tctttcaata tcgttttaaa
     1321 agcttctatc ggaagtatta ttgaagaagc atatagaaat acaaaacatt actccattgc
     1381 taaatggcag aatttgcgtc aaaaaatgga aaaagatatc aaaactgtca atcctagtct
     1441 agaaacatcg gtaaccccta gactctattt agatgaaaat gttctaaatg gtcttgatga
     1501 atttcgatat catttaatga aagaagatgg tgctactaga ttaccaaggc ttagttacat
     1561 cattaaactt gttgtttatt cctattggaa gacacaacaa taacctaagc aaaacctgct
     1621 taggttcctt ataaaaacaa atgtat
//
LOCUS       DAVKHZ010000147         1567 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000147 DAVKHZ010000000
VERSION     DAVKHZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.147"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..1331)
                     /locus_tag="VWW99_002095"
     CDS             complement(<1..1331)
                     /locus_tag="VWW99_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU9539864.1"
                     /translation="MFKVLRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASINAAVFSGNMIVNGAPAINASLNIA
                     KSETKVYTGTGKDSFYNIPIYYQLTVTNDGSKLTFTYTVTYVNPTTKILGNLSNMRPG
                     YSIYNTGTSTQTMLTLGSGLGIPSGVKNSITDKNGRQVRYYNTSTMTKRGSGYTWVNG
                     AQMNGWYAKKEYGLTSSWTVPITGTDTSFTFTPYAAKTDRIGINYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASESASTSASA"
     gene            complement(1329..1442)
                     /locus_tag="VWW99_002096"
                     /pseudo
     CDS             complement(1329..1442)
                     /locus_tag="VWW99_002096"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          445 a          327 c          305 g          490 t
ORIGIN      
        1 gctgaagcac tcgttgatgc ggattcagac gcacttgttg atgctgaagc cgacgcactc
       61 gttgatgctg aagccgacgc acttgttgat gctgaagctg aggcgctttg actagcactt
      121 actgagagtg acttagactg tgaaagtgac tgactggtcg tgctagattc aactaccttt
      181 cctctaccct tgaagtagtt aattccaatt ctatctgttt tagcagcgta aggggtaaat
      241 gtaaaggatg tatccgttcc agtaattggt acagtccaag atgatgttaa tccatattct
      301 ttcttagcat accaaccatt catttgggca ccatttaccc aagtataccc actaccccgc
      361 ttcgtcattg tagatgtatt atagtatcga acctgtctac catttttgtc agtaatggag
      421 ttctttacac ctgaagggat accaagacca ctgccaaggg ttaacattgt ttgtgttgaa
      481 gtacctgtat tatagataga atacccagga cgcatatttg aaagattacc aagaatcttt
      541 gttgtaggat tcacatacgt aaccgtatag gtaaaggtca attttgaacc atcatttgtc
      601 actgtcaatt gatagtaaat tggaatatta taaaatgaat ctttacctgt acccgtgtaa
      661 acttttgtct cacttttagc aatgtttaga cttgcattaa ttgcaggagc gccatttaca
      721 atcatattgc cagaaaaaac agcagcattt atagaagcca gcaattgctc gatggaatcc
      781 actgaacgct tgcgtcgctt agcataagat tggagattga catttgtaac tgatgctaca
      841 taatcactag ctggtttctt acgatcttct tcgaccttct tagcagttgc ttctgtagcg
      901 gcagctgttt gtgaacctac cacagtagat gatgcagaga tacttgtcga agccgatgta
      961 cttgctgatt ctgatgcact tgtactcgct gaggtcgaag cgctggttga ggctgactca
     1021 gatgcagatg tacttgccga ctctgaagct gacaaactag ttgaactcgc tgaattactt
     1081 gtagatatcg tacctagaac tactgtacca tttgttgcca aagcatccgt tttctctaca
     1141 gttttttcaa gtactgctga ctcatttgta aataccttcg tttgagttgc aacggttcca
     1201 cttatgacag ttcccgcagc aactatcccc ttgaggatat caagcccagt aattgaatga
     1261 cttactttat cttctaccgt ttcggtcatg acctgagtag tatcaacacc acctcgcaag
     1321 accttaaaca agccaaaaag agaagtcgag gcccgtagcc aatgcttccc tgatttgatt
     1381 aatttgaacc gagttactcg gtctgtttct ctatattcac ccttctgacg tctaaaaaat
     1441 attttgaaat ttcctttcga gtattatttt catgtatgga tataaataaa ttaccatact
     1501 ataattatag aatagggaac ccaaaatagc aagaaacttg aggtattttt gctaaaaaaa
     1561 aaaagca
//
LOCUS       DAVKHZ010000148         1524 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000148 DAVKHZ010000000
VERSION     DAVKHZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.148"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            83..>1524
                     /gene="pcpA"
                     /locus_tag="VWW99_002097"
     CDS             83..>1524
                     /gene="pcpA"
                     /locus_tag="VWW99_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9539865.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDEKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGS"
BASE COUNT          599 a          206 c          244 g          475 t
ORIGIN      
        1 attgaataaa actttttata acaacggcta gcaaagttat aattttatat ctattagaaa
       61 ataaatatgt aaggaaattt aaatgaaaaa aactacaata ttatcattaa ctacagctgc
      121 ggttatttta gcagcatatg tccctaatga accaatccta gcagatactc ctagttcgga
      181 agtaatcaaa gagactaaag ttggaagtat tattcaacaa aataatatca aatataaggt
      241 tctaactgta gaaggtaaca taggaactgt tcaagtgggt aatggagtta ctcctgtaga
      301 gtttgaagct ggtcaagatg aaaaaccatt cacgattcct acaaaaatca cagtaggtga
      361 taaagtattt accgttactg aagtagctag tcaagctttt agttattatc cagatgaaac
      421 aggtagaatt gtctactatc ctagctctat tactatccca tcaagcataa aaaaaataca
      481 aaaaaaaggc ttccatggaa gtaaagctaa aactattatt tttgacaaag gcagtcagct
      541 ggagaaaatt gaagatagag cttttgattt ttctgaatta gaagagattg aattgcctgc
      601 atctctagaa tatattggaa caagtgcatt ttcttttagt caaaaattga aaaagctaac
      661 cttttcctca agttcaaaat tagaattaat atcacatgag gcttttgcta atttatcaaa
      721 tttagagaaa ctaacattac caaaatcggt taaaacatta ggaagtaatc tatttagact
      781 cactactagc ttaaaacatg ttgatgttga agaaggaaat gaatcgtttg cctcagttga
      841 tggtgttttg ttttcaaaag ataaaactca attaatttat tatccaagtc aaaaaaatga
      901 cgaaagttat aaaacgccta aggagacaaa agaacttgca tcatattcgt ttaataaaaa
      961 ttcttacttg aaaaaactcg aattgaatga aggtttagaa aaaatcggta cttttgcatt
     1021 tgcggatgcg attaaacttg aagaaattag cttaccaaat agtttagaaa ctattgaacg
     1081 tttagccttt tacggtaatt tagaattaaa agaacttata ttaccagata atgttaaaaa
     1141 ttttggtaaa cacgttatga acggtttacc aaaattaaaa agtttaacaa ttggtaataa
     1201 tatcaactca ttgccgtcct tcttcctaag tggcgtctta gattcattaa aggaaattca
     1261 tattaagaat aaaagtacag agttttctgt gaaaaaagat acatttgcaa ttcctgaaac
     1321 tgttaagttc tatgtaacat cagaacatat aaaagatgtt cttaaatcaa atttatctac
     1381 tagtaatgat atcattgttg aaaaagtaga taatataaaa caagaaactg atgtagctaa
     1441 acctaaaaag aattctaatc agggagtagt tggttgggtt aaagacaaag gtttatggta
     1501 ttacttaaac gaatcaggtt caat
//
LOCUS       DAVKHZ010000149         1430 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000149 DAVKHZ010000000
VERSION     DAVKHZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.149"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            1..378
                     /locus_tag="VWW99_002098"
                     /pseudo
     CDS             1..378
                     /locus_tag="VWW99_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            439..1402
                     /locus_tag="VWW99_002099"
                     /pseudo
     CDS             439..1402
                     /locus_tag="VWW99_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
BASE COUNT          442 a          233 c          301 g          454 t
ORIGIN      
        1 ttaaaaggtc agttaagaag aagatattag aggatttctt tggttgcagg tctaaccaat
       61 ggtgagttaa tcgctccaat gacttacgaa gagacgatga cgagcgactt ttttgaagct
      121 tggtttcaga agtttttctt accaacatta aacacaccat cggttattat tatggataat
      181 gtaagattcc atagaatggg gaagctagaa tttttatgcg aggagtttgg gcataaactt
      241 ttacctcttc ctccctactc gcctgagtac aatcctattg agaaaacatg ggctcatatc
      301 aaaaagcacc tcaaaaaggt attaccaagt tgcaatacct tttacgaggc tcttttgtct
      361 tgttcttgtt tcaattgact atatatcacg gatacagttg ggaaagaagc tattaattat
      421 ttggagagtc cttttgggat gaacatgtca aatattacta tcagatgggc aagaaatgga
      481 tctgagtcaa acgatgcctt cttatgctca agtagtgagt ctttatgatg atacaagtat
      541 ctctgttagt aataatgaga cggacaaagt gttggctgga tccctctata ctgatacaaa
      601 tgaacaggga ttaacgattc caagcagttt actaaaaaat tggaatgaac agacaggaaa
      661 aaatttgaca gctaatgatc ttattggcaa atcagtctca gccagcattg tagaaagtgc
      721 tgccgaaact agtaagattg ctcaatttca aacgaagatt gtacgtgtaa tcaatgatga
      781 agatgacatg gaggacagca acagtttcat gctgtctcat caaatggaaa cgattttgaa
      841 agaggctgga tttacgaaag ctgtatctta ttttatcttg gaactcaaag atccatcaca
      901 gacaaaagta gtaacagaag aattacagaa aaataagaag tatactgtgc tttctcaaca
      961 gagagttctt gatattgtga ttacctttat tcgtgttatt cagggattat tgattgtgct
     1021 ttcatcacaa gctattgtgg tagcagcggt tatgattggt atcattattt acatcaatat
     1081 catgcaacgt tccaaggaaa taggtgtcat gaaagcagtt ggttatcaga atcgtgatgt
     1141 caaaggaatt tttatttacg aggctatctg gattgtaggc atcgccttgc tgctggcatt
     1201 tttggttgca caaggggtgg gaagtttggc gaatgcgatt gtaagtcact tttacccatc
     1261 catcactaag gtttttgaat taaatctttt atctgtttta ggaactctag ttttcgctct
     1321 attacttggt tatgtctcag cctacttccc ggcgcgtaag attagtaaaa tggatcctgt
     1381 agaaccgtta cgctatgaat agtaagggat tccttgattt acagtcttaa
//
LOCUS       DAVKHZ010000150         1370 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000150 DAVKHZ010000000
VERSION     DAVKHZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.150"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..>1370
                     /locus_tag="VWW99_002100"
     CDS             <1..>1370
                     /locus_tag="VWW99_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEU9539866.1"
                     /translation="DDNNSDLIETSNTELIFEKTSNVILTGTGGIGKSMLVKHIFINQ
                     VQQATSIPIFIELKSLNESDFSENELVDFIYQEVQNHHLNLEKKYFKATLEAGRYTII
                     FDGLDEVNPSKRSWLDKEIKEFVTLYNSNRYVLSSRPSEEFIGWNQFAEYEIKKLNRE
                     QALSLIDKLNYDEKVKRRFYRELKDHLYDTHESFASIPLLLTIMLMTYEAGASIPNNL
                     TDFYNQAFYTLYQRHDASKSGYKRELKAKLTPEEFRNILAYIGLKTFFEGKVDFDRTT
                     LDEIITKYCFKNNLELKTNDIVYDATHSACMMLQEGVSLKFSHRSFQEYFAAVGVNQL
                     DDKLQKQILIKWSEADRNNIVAHKTFMNALFKIQKERTYKNLCIPIIERMDEKYSRMG
                     DITEKIVTCFNYFICRKDSRENKLELHFLLTKEVHFYFSLQFLIFANLGIDILSISDY
                     ENIEKL"
BASE COUNT          528 a          168 c          223 g          451 t
ORIGIN      
        1 tagatgataa taattcagat ttaatagaaa cctctaacac agaattgatt tttgaaaaaa
       61 catctaacgt tattttgaca ggaacgggtg ggattggtaa atcaatgctt gtaaagcata
      121 tttttatcaa tcaagtacaa caagctacat caattccaat ttttattgaa ttaaaatcac
      181 ttaatgaatc tgatttctca gaaaatgaat tagttgattt tatttaccaa gaggttcaaa
      241 atcatcattt aaatttagaa aagaaatatt ttaaagcgac attagaggct ggaagatata
      301 caataatttt tgatggttta gatgaggtta atccttcaaa acgttcttgg ttggacaaag
      361 aaattaaaga atttgtaacg ttatacaata gcaatagata tgttttgtcg tcaagaccgt
      421 ctgaggaatt tattggttgg aatcagtttg ctgagtatga gataaaaaaa ttaaatagag
      481 agcaagcgtt atccttaatt gataagttga attatgatga gaaagtaaaa aggcgtttct
      541 acagagaatt aaaggatcat ttatatgata ctcatgaatc ttttgcttct atcccactcc
      601 tattaacaat tatgttaatg acttacgaag ctggtgcttc tattccaaat aatttaactg
      661 atttttataa tcaggccttt tatacattgt accaaagaca tgatgcatct aaatcaggat
      721 acaaaagaga actaaaagca aaattaactc ctgaagaatt tcgaaatata ttagcctaca
      781 ttggtttaaa aacattcttt gaaggaaaag tagatttcga tagaactaca cttgatgaaa
      841 taattactaa atattgtttt aaaaataacc ttgaattaaa gacgaatgat atagtttatg
      901 atgcgactca tagtgcttgt atgatgcttc aagaaggcgt tagtttaaaa ttctctcacc
      961 gttcattcca agaatacttt gctgcagtag gtgttaatca gctagatgat aaactccaaa
     1021 aacagatact aattaagtgg tctgaagcag ataggaataa tattgtagca cataaaactt
     1081 ttatgaatgc actttttaaa atacagaaag aaaggactta caaaaattta tgtattccaa
     1141 tcatagaaag aatggatgaa aagtatagca gaatggggga tatcacagag aaaattgtta
     1201 cgtgtttcaa ttactttatc tgtagaaaag atagtagaga aaacaaatta gaattgcact
     1261 ttttactaac aaaggaagtt cacttttatt ttagcttgca atttttaata tttgctaatc
     1321 taggtattga tatattatca ataagtgatt atgaaaatat agaaaaatta
//
LOCUS       DAVKHZ010000151         1367 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000151 DAVKHZ010000000
VERSION     DAVKHZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.151"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(153..338)
                     /locus_tag="VWW99_002101"
     CDS             complement(153..338)
                     /locus_tag="VWW99_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539867.1"
                     /translation="MAKLTNFYLNKQNEINLPRLAKFFKEKNMLERENRTWVINLAIK
                     ELFESLESEIIDFENNN"
     gene            complement(1234..>1367)
                     /locus_tag="VWW99_002102"
     CDS             complement(1234..>1367)
                     /locus_tag="VWW99_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539868.1"
                     /translation="GSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT          492 a          238 c          159 g          478 t
ORIGIN      
        1 tataaatcac gtatatatta tggttatatc atatcatgcc gaggaggaaa aatcaagacc
       61 tcaaacatta catctttgtt gcatatgtat tacatatcaa atacatgaat atatatttat
      121 tgttgcaaaa aaaaagacgc tcagtgcgtc aattaattat tattttcaaa atctataatc
      181 tcagactcca gactttcaaa aagttccttt atcgctagat taatcaccca agtcctattc
      241 tctctttcaa gcatattttt ttctttaaaa aatttagcta gtcttggtaa attaatttca
      301 ttctgtttat tcaaataaaa atttgttaat ttcgccataa tattcctcct acataatata
      361 tatcataaaa acacatactt ttcaagtaat tcctatccaa aaacttctct acacactttg
      421 tacatctgat ataatacctt tgatgaacta tcataacgag taattttgtt taatacttct
      481 acaaactaat gtactccata aattgaaact acaacgccct tatctaatta agaaaatatc
      541 taacctatga taaagcaata ctaataagtg ctatattctg atttccaaag aatattaatt
      601 tatcgtaact gattcctaac tgattaaatt tcaataaatt catatttctc tatttaccat
      661 ttattttatt ttaggtagaa aagctatcaa atcaaggttt tcaaatatca ttacatacct
      721 ttaaaacaac atcttcttca cagaagtgaa gtaagcattc aatacgaatc aatacagaag
      781 aaaaacgctt gatcagcgtt ttttcttttt gttaatttca ctatattgca agggaaatca
      841 ttcgaatgac tacctttttt gaaataagaa tacggattcg gacaattaaa agcaagagga
      901 actcaaatgt aagttggggt tccttttttg tatatttttc aaataacgcc tctacactat
      961 ttgtagcaaa ttcaccaact acagttgtat cttagttaaa ataagttaga atatgtaagt
     1021 gagtaccaga tataccaaga catcgtcacc atctaaggta tattcaaaat acaaaagttg
     1081 accaactaga tttctgaata tccttatata tccattctta aaattggttt aaatagcgta
     1141 gtcttttaaa ctagttttga gaatccaaaa aatcttccta catatgtaag aagatttttt
     1201 agttcagaat gattagattt agctaatgga tacctatcct acccactcac cgttagcatt
     1261 gactcgatag ccatcgggtg tagtcgtgtt tactaataaa tctcctgaat tataggtata
     1321 gtaccattta ccagaaactg taaaccaacc agtagccatt gaacctg
//
LOCUS       DAVKHZ010000152         1288 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000152 DAVKHZ010000000
VERSION     DAVKHZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.152"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            180..1208
                     /locus_tag="VWW99_002103"
     CDS             180..1208
                     /locus_tag="VWW99_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9539869.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     FRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILAEIRNI
                     HAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARFSPAFK
                     AYLKTKLE"
BASE COUNT          394 a          279 c          239 g          376 t
ORIGIN      
        1 tggattagtt catttcctcc aatcgcttga cgatttccga aagggtctaa ccacagcttt
       61 ttgtttcaat tggtaatgag tttgataacg catagacgtt ttatctcgtc taaggtaaca
      121 aggcaaaaag ttcgtggata accaagtaga cttgaaatac tatatctgga ggtactccca
      181 tgctttatgt gggcattgat gtcgctaaaa ataaacacga tgttacagcc ttgaatgttc
      241 caggaaaaac tgttcttaaa ccactcactt tttcaaataa taaagctggt tttgaactct
      301 tagatctgtc ttttcgacag ctcaaccaag actgtctcat cgctcttgaa gatactggac
      361 attatgcctt taatctactg aacttcctgc atgaacaagg atataaagtt tacacctaca
      421 ctcctttgct catcaaggaa tttgccaaat cattatcact tcgcaagact aaaacggata
      481 agaaagacgc tcatggcatc gctcttaaac ttctttctga ccccaatcgt gaacaatttc
      541 aacacgataa tcggcaagta gaactaaaaa tactggctag acatattcat cgtctcaaga
      601 aaaaacagtc tgattggaaa gtacaataca ctcgttgtct tgatatcatc tttcctgagt
      661 tggataaaat cgttggaaag cattcagaat atacctacca actcttgaca tgctacccta
      721 atcctcagaa aaggcttgag gcaggatttg ataagctgat agaaattaag cgattgaccg
      781 cttctaaaat tcaggatatc ctctcagtcg cacctcgttc tatcggaaca acatctcctg
      841 ctcgtgaatt cgaaatcatc gaaatcatca aacattacaa gaggctcatt gacaaggcgg
      901 aaacatgtgt caatgacttg atggctgagt tcaactcggt catcacgacg gttactggga
      961 ttgggggtcg tttaggggcg gtcattttag ccgagattcg aaatattcat gcctttgata
     1021 atcctgctca attacaagct ttcgctggac tggattcttc tatttatcag tcaggtcaga
     1081 ttgatttagc tggaagaatg atcaaacggg gttcccctca tctgcggtgg gcactcatac
     1141 aagctgccaa agcatgcgct cgcttttcac ctgcttttaa ggcctatctt aagactaagt
     1201 tagaataagg aaaacattac aatgtagcca tcatccacct tgcaaaaaaa cttatccgaa
     1261 ccctgtttta tatcctaaaa aagagctg
//
LOCUS       DAVKHZ010000153         1193 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000153 DAVKHZ010000000
VERSION     DAVKHZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.153"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            343..1193
                     /locus_tag="VWW99_002104"
                     /pseudo
     CDS             343..1193
                     /locus_tag="VWW99_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836677.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
BASE COUNT          550 a          163 c          212 g          268 t
ORIGIN      
        1 aaaaatcatc taaagatgaa ataacataat tgtcccttgg ttacaagggc gcaattatac
       61 accctaaagg gtgcttgcgt cctgcggagt caaacccttg cagggagcaa caaccattcg
      121 ctttttaaaa gtcaagggta aataaactcg ctaaggctcg cccttgactt ttaaaatttg
      181 aaatgaaagt aacaattaag ccgacatact gaaacaacaa aaattaattc agtagtcggc
      241 ttttttaatt ttatcatttc aaaacgctca ttcacaaaga ggatataaaa aaactattaa
      301 gcctccacct aaaacaacaa agaaagaaag gaagtgataa aaatggcaga cagttttcat
      361 ttttcagtaa acataatatc aagaggaaaa ggcaaaagtg cagtagcaag tgcagcatat
      421 ataagtggag aaaaaataaa aaatgaatgg gacggagtaa cccatgacta cacaagaaaa
      481 gaaaaaatat tagtaaaaaa tataatattg cctgatcata taccaaaaga atttaatgat
      541 aggtctacct tatggaataa agtagaaatg gcagaaaaaa attctaatgc acaactagca
      601 agacaattca taataggact accaaaagag ttatctttaa gtgaaaataa aaacctggtt
      661 gaaagatata taaaagaaaa tttaacatca caaggaatga tagtagatta tgcaattcat
      721 gatgagagtc aagataaaaa tggaaatatc cattgtcata taatgaccat aatgcgacca
      781 ataaacgaaa aaggagagtt cttagccaag tcaaaaaaag aatatatcct agatgaaaaa
      841 ggagaaaagt attaaacaaa aatggaaaac caaagacaag aaaagtagaa ctaacaacct
      901 ggaacgatac aggcaatgta gaaaaatgga gagaaaattt ttcagatctt tgtaacaaat
      961 atttagaaag agcaggagca gaaaaaagag tagaccatag gagttttaaa agacaaaatt
     1021 cagattatct accgacaatc catttaggaa cagcagcaag tgcaatggaa agaaagggaa
     1081 tagaaactga caagggaaac tataatagag aaataagaaa atataatcaa cttgtaaaaa
     1141 caataaaaga agagataaaa acattaaagg gttggatagg aaatttatta gat
//
LOCUS       DAVKHZ010000154         1034 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000154 DAVKHZ010000000
VERSION     DAVKHZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.154"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..341
                     /locus_tag="VWW99_002105"
     CDS             <1..341
                     /locus_tag="VWW99_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU9539870.1"
                     /translation="HALRKETQIEEKKLPLFGTPGFYETNLNLLNNQPEDIMGLVLLL
                     YWSQNLDDFNVFSKMSFLEAMEYAMNFNSRRLDTLDKSDWLYDIYKKAFNYKYDTFSE
                     LKKDFIEVLK"
     gene            355..>1034
                     /locus_tag="VWW99_002106"
     CDS             355..>1034
                     /locus_tag="VWW99_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539871.1"
                     /translation="MNCFLKMNNVSVRYDDVIALKDITLQINKGDFIGLLGSNGAGKS
                     TLINSIVGFQEIYLGEIEYCDKDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLN
                     LAGKFGKNAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVQIARAIAHNPDFYILDE
                     PTVGLDTESAEKFLMYLKDKSLEGKTIIISSHDINLLEKFCKKILFLQNGSISFFGDM
                     RDFVDNST"
BASE COUNT          362 a          119 c          193 g          360 t
ORIGIN      
        1 agcatgccct aaggaaagaa actcaaattg aagaaaaaaa gttgcctcta tttggaactc
       61 cagggtttta tgaaaccaac ttaaatttat tgaataatca accagaggat attatgggat
      121 tggttttgtt attatattgg agtcagaatt tagatgattt taacgtcttt tcaaaaatga
      181 gttttttaga agcgatggag tatgcaatga attttaattc gcgtcgctta gacacacttg
      241 ataaatcaga ttggctttat gatatttata aaaaagcgtt caattataaa tatgatactt
      301 tttctgaatt aaaaaaagat tttatagagg tattaaaata aaggtagggg tgttatgaat
      361 tgtttcttga aaatgaataa tgtaagtgtt cgttatgatg acgtaatagc tttaaaagat
      421 ataactttac aaataaataa gggagatttc attggcttat taggttcaaa tggtgcaggt
      481 aaatctacgt taattaattc tattgtaggt tttcaagaga tttatttagg agaaatagag
      541 tattgtgata aagatttgat agttagttct caaccttttg ctcatttagg ctttactcct
      601 caaaccacag taattgattt ttatactact gtgaaggaca atgtaatatt ggggctgaac
      661 cttgctggaa agtttgggaa aaatgctgag aagttgtgtc aaatagcctt agaaattgtt
      721 gggttagctg ataaaaaaaa taatttggta gaaacattgt caggtggaca actgcaacgc
      781 gtccagattg ctagagcaat agctcataat ccagattttt atattttaga tgaacctacc
      841 gttggtttag atactgaatc tgccgaaaaa tttttaatgt atttaaaaga taagagtttg
      901 gaaggaaaaa ctattatcat atcttcacat gacataaatc tactcgaaaa gttttgtaaa
      961 aaaatacttt ttttacaaaa tggctccata tcattttttg gtgatatgcg tgactttgta
     1021 gataattcaa ctat
//
LOCUS       DAVKHZ010000155         1029 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000155 DAVKHZ010000000
VERSION     DAVKHZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.155"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(21..821)
                     /locus_tag="VWW99_002107"
     CDS             complement(21..821)
                     /locus_tag="VWW99_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9539872.1"
                     /translation="MKADQLRHKSDLGLRGLAIIAKNEIIAFFRSKGLIISQFLQPIL
                     YVVFIIIGLNSSIKNIQFNDIKTSYAEYTIIGVIALLIIGQMTQVIYRVTIDKKYGLL
                     ALKLCSGVRPLYYILGMSIYSILGLIVQEIIIYIITLAFEINIAMDRFFYTVLLSIVV
                     LLFWDSLAILLTMFINDYRRRDIVIRFVLTPLGFTAPVFYLIDSAPSIVRWIGQLNPL
                     TYQLTILRNFYFKNSTTLELVFLLLTSLLVLISVSFIIPKIKLILIER"
     gene            complement(818..>1029)
                     /locus_tag="VWW99_002108"
     CDS             complement(818..>1029)
                     /locus_tag="VWW99_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9539873.1"
                     /translation="ISRYQIEFLENFRFKVHIEDNDSFTIEVPIEENILDVINEVGKA
                     CEIKNFSTSKLTLQESYLQRIGGEK"
BASE COUNT          391 a          165 c          121 g          352 t
ORIGIN      
        1 aaagttggat gacccaactt ttatctttct atcagtatca attttatctt tggtataata
       61 aaagatacag atataaggac aagtaatgat gttaacaata agaaaactaa ttccaaagtt
      121 gttgaatttt taaaataaaa gtttctcaaa atagttaatt gataagttaa gggatttaac
      181 tgaccaatcc atctcacaat actaggagca gaatctatta agtagaaaac aggagctgta
      241 aaaccaagcg gtgttagtac aaaacgtatt acaatatcac gtcttctgta atcattgata
      301 aacattgtaa gtaaaattgc aagggagtcc caaaataata aaacaacaat agataacaaa
      361 actgtataaa aaaatctatc cattgcgata tttatctcaa acgctaacgt aattatatat
      421 ataataattt cttgaactat caaccctaat atagaataga tactcatccc taaaatataa
      481 tataaaggac gaactccact gcataactta agagcaagta gcccatattt tttatctatt
      541 gtcaccctat aaataacttg agtcatctgc ccgattatca ataaagctat aacaccaatg
      601 attgtatatt ctgcataaga ggtttttata tcattaaact gaatgttctt tatcgaagaa
      661 tttaatccta ttattataaa aacaacatat aagattggtt gtagaaactg agaaataatt
      721 aaacctttac ttctaaaaaa agcaataatc tcatttttag caataatcgc tagacctctt
      781 aaacctaagt ccgatttgtg ccttaattga tcagccttca tttttctcct cctattcttt
      841 gcaaataact ttcttgtaag gttaatttac ttgttgaaaa gtttttaatt tcacatgctt
      901 ttcctacctc attgataaca tctaagatat tttcttctat agggacttct attgtaaaac
      961 tatcattatc ttcgatgtga actttaaatc taaaattttc taaaaattca atttgatatc
     1021 tagaaattc
//
LOCUS       DAVKHZ010000156         1000 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000156 DAVKHZ010000000
VERSION     DAVKHZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.156"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            42..236
                     /locus_tag="VWW99_002109"
     CDS             42..236
                     /locus_tag="VWW99_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539874.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLREQVAYLTQKLYGKSSEKSVCPF
                     GQLSLFEEEQNMEEDSDLPS"
     gene            220..606
                     /locus_tag="VWW99_002110"
     CDS             220..606
                     /locus_tag="VWW99_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEU9539875.1"
                     /translation="MTYPVEREEITYKRKKAKGKRQAILAQFDSEEVHHQVEESICPD
                     CQDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQACSDKNPSDKIVKAPVPKAPL
                     AHSLGSASIIAHTIHQKFNLKVPNYR"
     gene            721..912
                     /locus_tag="VWW99_002111"
     CDS             721..912
                     /locus_tag="VWW99_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539876.1"
                     /translation="MLEQALLHADETSYRVLESDSQLTYYWTFLSGKSEKQGITLYHH
                     DQCRSGSVVQEFLGDYSAL"
BASE COUNT          346 a          178 c          199 g          277 t
ORIGIN      
        1 aataaagtta ggaaataagg aggaaagtct atgggagaag attgaaaatc attcaacaac
       61 agagtgctat aattgatagt ctcactaatg aactttccct tctgcgtgaa caagtggctt
      121 atctaacgca aaagctctat ggaaaatcct ctgagaaaag tgtttgccct tttggacaac
      181 tcagtctttt tgaagaggaa caaaatatgg aagaagactc tgacttaccc agttgaaaga
      241 gaagaaatca cctataaacg taagaaagct aaagggaaac gtcaagctat tcttgcccaa
      301 tttgattcag aagaagttca tcatcaagta gaagagagca tttgccctga ttgtcaggac
      361 gatttaaaag agattggagc gagccttcaa cgacaagaat tggtctttat tcctgcgcaa
      421 ttaaaaagag tggatcatat ccaacacgct tacaaatgcc aagcatgcag tgataaaaat
      481 ccaagtgata aaattgtaaa agctcctgtc cctaaagccc ctttggcgca tagccttggt
      541 tcagcctcta ttatcgctca caccatccat cagaagttta atctgaaggt acccaattat
      601 cgctaagaag aagattgggc taagatgggt ttaccaatca cacgtaagga aatttctaat
      661 tggcatatca agacgagtca atactattta gagtctcttt ataacctttt atgagaaaag
      721 ttgttagaac aagctcttct tcatgcagat gaaacctctt atcgggtctt agaaagtgat
      781 agtcagctga cttactattg gacttttttg tcaggtaaat cagagaaaca agggattacg
      841 ctttaccacc atgatcagtg tcgaagtggt tcagtagtac aagaattcct aggagattat
      901 tcggctctat aatatttgta gtgggtaaat ccactataga tattatggag cctattttat
      961 tgtagaaaaa aagtcccata agatctataa tgaaaagcga
//
LOCUS       DAVKHZ010000157         1000 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000157 DAVKHZ010000000
VERSION     DAVKHZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.157"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            80..828
                     /locus_tag="VWW99_002112"
                     /pseudo
     CDS             80..828
                     /locus_tag="VWW99_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            841..>1000
                     /locus_tag="VWW99_002113"
     CDS             841..>1000
                     /locus_tag="VWW99_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9539877.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKF"
BASE COUNT          347 a          112 c          196 g          345 t
ORIGIN      
        1 ataaggtgaa tttttttgat gatgtggaat ttaatcagac acttagtgag tttttagata
       61 aattgtagag ggaatggtta tgagtaacaa tactaagtat atctttttat ctccacattt
      121 agatgacgca attttttcct gtggtgatta tatttcaaaa ttaacttcag aaggagaaat
      181 tgtacttgtt attactatat tttctggtta ccctttgagc caacagctac agccctctgc
      241 taagcaattt cataagttgt gtaaccttgg gaaatatccg attgaagaaa ggaagaaaga
      301 agacaggtta gcttgtgaac gtctacaatg tgattttaga cacttatcgt actatgaatg
      361 tctctatagg aaagatagaa atggcaattt tctataccgt catatctata gtgaattaaa
      421 aaatgagggc acattaaaga atgatatcat aaaagagctg ttaatgcatt tagacgacaa
      481 atgtgtcgta tattgtccgc tttcacttgg tgaccatgtt gatcatgttt ttgtgaatag
      541 tattggaagg gcgcttgaat ttatgcgtta taaagtaata tattatgagg acttcccata
      601 tgtaagtgat agtagtatgg ttagttatat gggaaaaact aaagaattaa aaatgtatca
      661 agaggaactt gatgaaaaaa ttatattgat agaatatcat caatattgtg ctataaatct
      721 cagattttga taatctggaa atcagttgaa aaacttttaa acaatattaa agagttgtat
      781 ttaaggaatg gggcagctta ttctattaga ttttggatta agaaataagg aagtagtttt
      841 atgaatatag cagtaattgg tttggggcat gttgggctgg cctacgcgtt actatttgca
      901 tctaaatata aagttgttgc atatgatata gattctgtaa aaataaataa tttaaaaaag
      961 ggcattcttc catctaaaaa tgaagagctt atgaagtttt
//
LOCUS       DAVKHZ010000158          928 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000158 DAVKHZ010000000
VERSION     DAVKHZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.158"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            98..280
                     /locus_tag="VWW99_002114"
     CDS             98..280
                     /locus_tag="VWW99_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9539878.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLCFNEKFGTENT"
     gene            290..589
                     /locus_tag="VWW99_002115"
                     /pseudo
     CDS             290..589
                     /locus_tag="VWW99_002115"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            653..>928
                     /locus_tag="VWW99_002116"
     CDS             653..>928
                     /locus_tag="VWW99_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9539879.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRL"
BASE COUNT          387 a          115 c          154 g          272 t
ORIGIN      
        1 aggatagaaa tgaccgaatt agaaagaaaa aatcgaaaaa ttagctaaga aatattctga
       61 taacttaaac atcaaagttc aagagagagt tcgtgaaatg gcaaatgata ataagagcca
      121 ttatttgata tacagagttt taggtatttc atttgaagaa ggagaaaata tcgatttgta
      181 tcaaaataaa ggtcgttttt tatacaaata tgctggttca tttttagaag aagctgcagt
      241 actatgcttt aacgaaaaat ttggtacaga aaatacttaa aaagttaaca ttcctaattc
      301 tgaaagtaca aaacctaaga cttttgaaat tgattgttta gtcggaaaaa aacacgcata
      361 cgaaataaaa tggtgggatg caactacaga tggagaccat ataactaaaa aacacactag
      421 aataaaagtt attcataaca aaggatatat accaattcgg ttaatgttct actatccaaa
      481 tagaactcaa gctataaaaa ttcagcaaac tttagaaaca ttgtataacg gtattggagg
      541 gaaatattat tatggagatt ctgcctggga acatttaaga gcagtgaccg gtattgattt
      601 acttagtatt ctaacagata ttgcaaataa aaaaaacagg ggtaaaatca aaatgacagt
      661 attaaaagga gataacttag aaatattaaa aactattgaa tcctcaagta ttgatttaat
      721 ctatatggac cctcctttct ttacacagaa aacccaaaaa ttatctaata acaaaaatat
      781 tatgtattca ttcgaagata cgtggacttc gattgaggat tacaaagaat ttttgtctgt
      841 aagattagaa gaatgcaaaa gagtgctaaa aaatagtggc agtattttcg ttcattgtga
      901 taaaattgca aatcatcata ttagatta
//
LOCUS       DAVKHZ010000159          919 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000159 DAVKHZ010000000
VERSION     DAVKHZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.159"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            1..919
                     /locus_tag="VWW99_002117"
                     /pseudo
     CDS             1..919
                     /locus_tag="VWW99_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          336 a          170 c          183 g          230 t
ORIGIN      
        1 ataaagtctg acatgaaggc tggactaaag atagagtttc tcttgaatac ggtctcccaa
       61 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
      121 agaaaacaag agggagagta cctgagagcg gagaatgcca tcctaaaaaa gttaagagaa
      181 ctccgattga aggaggaaaa agagaaagaa gaaagacaga aattgttcaa gaattaatga
      241 ctgagttttc gttagttctt cttctaaaag tcattaaact agctcgttcg acctactact
      301 atcacttgaa acagctagat aaaccagata aggaccaaga gcttaaagct gaaattcaat
      361 ccatttttat cgaacacaag ggaaattata cttatcgccg ggttcattta gaactaagaa
      421 atcgtggtta tctggtaaat cataaaagag ttcaaggctt gatgaaagta ctcaatttac
      481 aagctaaaac gcgacagaaa cgaaaatatt cttctcataa aggagacgtt ggcaagaagg
      541 cagagaatct cattcaaggc caatttgaag gctctaaaac aatggaacag tgctacacag
      601 atgtgactga atttgccatt ccagtaagta cttaaaagct ttacttatca ccagttttag
      661 atggctttaa tagcgaaatt atcgcctata atctttcaac ttcacccaac ttagaacaag
      721 tacaaacaat gttggaacag gcattcacag agaagcacta cgagaatacg attctccata
      781 gtgaccaagg ctggcaatac caacacgatt cttatcatcg gttcctagag agtaagggaa
      841 ttcaagcatc catgtcacgt aagggcaaca gccaagacaa cggtaggatg gaatctttct
      901 ttggcatttt aaaatccga
//
LOCUS       DAVKHZ010000160          883 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000160 DAVKHZ010000000
VERSION     DAVKHZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.160"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            83..880
                     /locus_tag="VWW99_002118"
     CDS             83..880
                     /locus_tag="VWW99_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9539880.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          233 a          152 c          193 g          305 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatgatttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaa
//
LOCUS       DAVKHZ010000161          881 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000161 DAVKHZ010000000
VERSION     DAVKHZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.161"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(8..81)
                     /locus_tag="VWW99_002119"
     tRNA            complement(8..81)
                     /locus_tag="VWW99_002119"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="VWW99_002120"
     tRNA            complement(95..168)
                     /locus_tag="VWW99_002120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="VWW99_002121"
     tRNA            complement(174..247)
                     /locus_tag="VWW99_002121"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VWW99_002122"
     tRNA            complement(263..336)
                     /locus_tag="VWW99_002122"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="VWW99_002123"
     tRNA            complement(346..431)
                     /locus_tag="VWW99_002123"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="VWW99_002124"
     tRNA            complement(439..510)
                     /locus_tag="VWW99_002124"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(533..605)
                     /locus_tag="VWW99_002125"
     tRNA            complement(533..605)
                     /locus_tag="VWW99_002125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Thr,seq:tgt)
     gene            complement(624..705)
                     /locus_tag="VWW99_002126"
     tRNA            complement(624..705)
                     /locus_tag="VWW99_002126"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Leu,seq:tag)
     gene            complement(711..783)
                     /locus_tag="VWW99_002127"
     tRNA            complement(711..783)
                     /locus_tag="VWW99_002127"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Lys,seq:ttt)
     gene            complement(808..880)
                     /locus_tag="VWW99_002128"
     tRNA            complement(808..880)
                     /locus_tag="VWW99_002128"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Asp,seq:gtc)
BASE COUNT          232 a          257 c          199 g          193 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      601 ccggctcatt tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc
      661 gccagatcct aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac
      721 tgggctcgaa ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga
      781 gtctaaaata acttgcgtta ccttaaacgg tcccgacggg aatcgaaccc gcgatcttcg
      841 ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc t
//
LOCUS       DAVKHZ010000162          818 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000162 DAVKHZ010000000
VERSION     DAVKHZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.162"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            469..>818
                     /locus_tag="VWW99_002129"
     CDS             469..>818
                     /locus_tag="VWW99_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU9539881.1"
                     /translation="MNVPIRDYYIPRYNTIPDQYIILEVISKNAKGGVYKVFNTQKRV
                     YSLLKEASDLSLVDFTNRDSVNRLINEREILVELEKEEFTPKVFNDFYIKNSYFVEFE
                     FIIADKLSEYNMVSG"
BASE COUNT          305 a           84 c          143 g          286 t
ORIGIN      
        1 tataaaatta gtgataattt tagtgataat tttagtgata attttagtga tgaacattgg
       61 ttatataatt tgccgtctaa tatcacccaa tctaagggat ttaaaataca cctgagcgct
      121 tcaatattaa atgctaattt ggttgctaaa aaattctttg attttatatt ttctagagag
      181 aagaagataa atttcaaaat tttggttagc attaaagaac tttctttgca aaatacaggg
      241 cttaatggtt attcacaagt cgggaaattc attacaattt atccaaaaga taataaagag
      301 tttcaacgtt tgttacacaa attagaaata ttgtataaag gtgtaaaggg tgtaaacata
      361 ccatcagatt tcagatttca acttagcgag gttgtatatt acagatatgg ggagttcgta
      421 aaagatagca ctttcaagga taaaagagat aagaaaatcc cttcaaatgt gaatgttcca
      481 ataagggatt actatattcc aagatataac actattcctg atcaatatat tattttggaa
      541 gtgatttcta aaaatgccaa aggtggagtt tataaagtat ttaatactca aaagcgagtt
      601 tattctttat taaaagaagc tagtgattta tcgttggttg attttacaaa tagagattct
      661 gtgaatcgtt taataaatga aagagaaata ttagtagaat tggaaaagga ggaatttact
      721 ccaaaagttt ttaacgattt ttacataaag aattcgtatt ttgtggaatt tgagtttatt
      781 attgcagata aattaagcga gtataatatg gttagtgg
//
LOCUS       DAVKHZ010000163          802 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000163 DAVKHZ010000000
VERSION     DAVKHZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.163"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            23..538
                     /locus_tag="VWW99_002130"
     CDS             23..538
                     /locus_tag="VWW99_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539882.1"
                     /translation="MNRLKELRQEKKLSQKELAENIGVHYRTLQNWENGESQIKPEKA
                     QQLADYFGVSVGYLLGYSEYRELEKALDKTIFSNYPDVETFLTQEIKELIGERTKDFY
                     EYIDKQFYESYKNTTVPPEIVVKHREDFYSSFLFLPARLQKFIALWSILTETEQENIG
                     KTIELLAMRGR"
BASE COUNT          310 a          133 c          133 g          226 t
ORIGIN      
        1 tctgtgaaag gtgtgataaa acatgaatag attgaaagaa ttaagacaag aaaaaaagct
       61 atctcaaaaa gagttagctg aaaatattgg ggttcattat cgtactcttc aaaattggga
      121 aaacggagaa agccaaatca aaccagaaaa agcccaacaa ctagctgact acttcggagt
      181 aagcgttgga tatctgttgg ggtatagtga gtatagagaa ttagaaaaag cattggataa
      241 aacaattttt tcaaactatc ctgatgttga aacattcctc actcaagaga taaaagagct
      301 aatcggagaa agaacaaaag atttttatga gtatattgat aaacaatttt atgaaagcta
      361 taaaaatact accgtacctc ccgaaatagt tgtaaagcac cgtgaagact tttatagctc
      421 gtttctattt ctccctgcta gattacaaaa atttatagct ttatggagca tactaacaga
      481 aactgaacaa gaaaatatag gtaaaactat tgaattatta gctatgagag ggagataaaa
      541 atccccccat attcgccaat agcaaacctt agcactaata tttttctttt agggtagccg
      601 aaagtgccga aaatgcgacc tatagcatac cacgcgccac aacgttcaac aatcatgaag
      661 cgcgtgtttt ctcagggttc actcttgatt cgttattaga ttaaatatgt aagcattagt
      721 tagcccttat ccaataaaaa ccaacattta ataaaaagat ataactcatt aactttttta
      781 atttttatct ttcaaacaaa ac
//
LOCUS       DAVKHZ010000164          788 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000164 DAVKHZ010000000
VERSION     DAVKHZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.164"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(43..189)
                     /locus_tag="VWW99_002131"
     CDS             complement(43..189)
                     /locus_tag="VWW99_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539883.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            347..565
                     /locus_tag="VWW99_002132"
     CDS             347..565
                     /locus_tag="VWW99_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9539884.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          251 a          116 c          146 g          275 t
ORIGIN      
        1 cgaaatctag tggaatgaat ctggaatagt ccatcgagct ttctaatact ctttgaaaat
       61 ctcttcaaac cacgtcaacg tcgccttgcc gtgcgtatgg ttactgactt cgtcagttct
      121 atccacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgctcttt
      181 ggttttcatt gagtataaca cattgttaga agttggttta aatttcctaa tcagtttgtt
      241 cacatttacc ttcgatatat tatatcccat agttaaggtt ggtcatacag atgattatag
      301 ccatggagcc gtaaaactta gtgtttcttt agttgacaaa gatgccatga aaaaaatatc
      361 tgtaactgta ataggatatt ttgaaataaa tatagatgaa aatatcaccg atattctata
      421 cgtaaatggt actgctattc tttatcctta tttacgttct attgtttcaa tagtttcggc
      481 aattgatagc agtgaagcaa tgttgctacc taccattaat gttttagagt tactagataa
      541 atctcaacct tttgaagaag aataatttat tagctcacta aattgagggt aaggaaaagt
      601 aaaagcagta agaaaaatgt cttgcattat acagcaactt tttgggaatg agtagatgga
      661 ttgaataaaa tttgattaag agtggatgat ttatctgtag attattattg gacagttagt
      721 cttgaagtag tctaagaatt aggttataat cagtagaagc cttgctaata atgaggaggt
      781 tagtttat
//
LOCUS       DAVKHZ010000165          784 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000165 DAVKHZ010000000
VERSION     DAVKHZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.165"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            193..330
                     /locus_tag="VWW99_002133"
                     /pseudo
     CDS             193..330
                     /locus_tag="VWW99_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            433..784
                     /locus_tag="VWW99_002134"
                     /pseudo
     CDS             433..784
                     /locus_tag="VWW99_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          274 a          138 c          147 g          225 t
ORIGIN      
        1 atttgtcttg ttcttatttc attttactat agaaagttag ctacagactg ctcaaaacac
       61 tgtttttagg ttgtagatag aactgacgaa gtcagtaaca tctatacgac aaggcgaagc
      121 tgacgcggtt tgaagagatt ttcgaagagt ataatactag actaaaatca aaaagcatta
      181 tacaatagta atatgaaatc gattaaagaa gaaatccaaa ccatcaaaac acttttaaaa
      241 gactctcgta cagctaaata tcataaacgc cttcaaatcg ttctatttcg tctgatgggc
      301 aaatcttata aagagattat agaactttta tagtggtttg aaataagatg tgaacaactc
      361 tatcaggaaa gtcaaactaa tttatagaaa tattttagca gccaaggtgt actgttatag
      421 attcaataca ctttagactg taatcaaaca acgatttggc gaaatgtaaa aaatatgagg
      481 agttcggact cgactctctc cttcaagaaa cacgtggtgg tcgtaaccat gcttatatga
      541 cggttgagca agagaaagtc tttcttgccc gccatttgaa ggctacagag gcaggagaat
      601 ttgttacaat tgatgcctta tttcaggctt ataaaaagga gttaggtcgt tcctacacac
      661 gtgatgcctt ctatcaactg ttgaagcgcc atggttggcg aaatattacg ccacgtccag
      721 aacatcctag gaaagcagat gctcaaacca ttgtcgcgtc taaaaataaa gtctcaattc
      781 aaga
//
LOCUS       DAVKHZ010000166          765 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000166 DAVKHZ010000000
VERSION     DAVKHZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.166"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..606)
                     /locus_tag="VWW99_002135"
     CDS             complement(<1..606)
                     /locus_tag="VWW99_002135"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEU9539885.1"
                     /translation="MQKPFKTIPEQIELLHKRGLIITDYKRAKKYLLTNNYYSIINGY
                     SKYFEQSPNKYRPGTNFSEITYSYFYDKEIKYSFLKAILEAEKHIKSAIAYVFSERFA
                     STPYFYLDINNYNKSNSDNVRKIKFVIRQMVKIIDKHKSSRQANSIQHYFSKHLEIPF
                     WVIIDYLTFGDMLAILQTLPISLQDKISKKFYSFICEHIKIN"
BASE COUNT          247 a          101 c          102 g          315 t
ORIGIN      
        1 attaatttta atatgctcac aaataaaact ataaaacttt tttgaaattt tatcttgtaa
       61 agaaattggg agagtttgta aaattgctaa catatctccg aaagtcagat aatcaattat
      121 tacccaaaat ggtatttcaa gatgtttact aaaataatgt tgaatactat tcgcttgtct
      181 acttgactta tgtttatcta ttattttaac catttgacgg atcacaaatt taatctttcg
      241 aacattatca ctattcgact tattatagtt atttatatct aagtaaaaat aaggtgtaga
      301 agcaaatctt tcagaaaata catatgcaat tgctgatttt atatgttttt ctgcttccaa
      361 tattgctttt aaaaaagaat attttatctc tttgtcataa aaataactgt aagtgatttc
      421 tgaaaagttt gttccaggtc tatatttatt tggtgactgt tcaaaatatt tactgtaccc
      481 attgataata ctgtagtaat tgttagttaa tagatatttt tttgctcttt tataatctgt
      541 aattattagg ccacgtttat gaagcaattc tatttgttct ggaatagttt taaatggttt
      601 ttgcaaaaaa aagcacctcc atatagaagg tgctattttc gcgttcccgt ttcactgaga
      661 ataatcgggc gctaattcct taagtacctt cattgtatga taattttaaa tgttagtcaa
      721 cagttttgtt caaaataatt gatttttctt tacttcgttt tgttt
//
LOCUS       DAVKHZ010000167          758 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000167 DAVKHZ010000000
VERSION     DAVKHZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.167"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            1..758
                     /locus_tag="VWW99_002136"
                     /pseudo
     CDS             1..758
                     /locus_tag="VWW99_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          228 a          170 c          127 g          233 t
ORIGIN      
        1 atagggattc acacaaagaa atcatcgcca aactggacta cgacgcccca tcttgccctg
       61 agtgcggaaa ccaattgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg
      121 aaacgactgg tatgcctact agaattctcc ttagaaagcg tcgctttaag tgctatcact
      181 gttcaaaaat agcggtcgct gagacttccc tcgtcaagaa aaatcaccaa atccctcgta
      241 tcatcaacca aaagactgct caaaagttga ttgaaaagat ttctatgacc gatattgccc
      301 atcagctttc catctcaact tcaactgtta ttcgtaagct caatgatttt cactttaaac
      361 atgatttttc ttgtcttcct gagattatat cttgggatga gtatgctttt acaaagggaa
      421 agatgagctt cattgcgcaa gattttaaca atctcaatat catcactgtt cttgagggta
      481 gaacacaagc tgtcatccga gatcactttc ttaaatatga tagagttgtc cgatgtcgcg
      541 tcaaaatcat tactatggat atgtttagtc cttactatga cttggctaaa cagcttcgct
      601 ttcaaatttc tagactcagg ctgaaacagt ctcccagact gtttcactcc cgaatgctaa
      661 aatcgttctt gaacgctttc atattgtaca acatcttagc cgtgctatga gtcgtgtgcg
      721 tgttcaaatc atgaatcagt ttcatcgaaa atcccatg
//
LOCUS       DAVKHZ010000168          746 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000168 DAVKHZ010000000
VERSION     DAVKHZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.168"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            139..>746
                     /locus_tag="VWW99_002137"
     CDS             139..>746
                     /locus_tag="VWW99_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539886.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTIL"
BASE COUNT          262 a          139 c          117 g          228 t
ORIGIN      
        1 taagctcatt ttaaaattac gttaactttt ttcacaaatt tatttcgact ttctttttta
       61 ctttttacat tgctatgata caatattttt aacaaaattt tattgtttgt taactttttt
      121 cacgaaaagg agaaaatcat gcgttatgat ttcggaaagg tctataaaga aatacgtgag
      181 tcaaaggggc tgacccaaga agaggtctgt gggggtgttc tctcaagaac cagcttatca
      241 aaaatcgaaa gtggcaagac aaccccaaaa tatgaaaata tggaatttct tctccgtcaa
      301 atcaatatga gttttgaaga gtttgaatat atctgtcaac tttatcaacc aagtcaacgc
      361 acagagatta tgcaaaccta tctcaatatg cgttccatta tcgggaccag cgatcttgtc
      421 aatttatttc aaaaatgcca agactacctc aagacccacc acgatctccc tatagaagaa
      481 atcagagata tgcttgaagt tgttatttat ctccgtcaac acggggcagg ggaactatca
      541 aaacacgcag aacaggttgt caaaaaactt tggaaaaaaa ttgaaaaaca agatacatgg
      601 tatgagagtg atctaaaaat cctcaatacc attcttttca gttttcccat tgaatacctc
      661 catctcatca ctggaaaaat attgcaacgc ctagaagtct ataaaaacta tcaacattta
      721 tatgacttgc gaatgacaat tctatt
//
LOCUS       DAVKHZ010000169          708 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000169 DAVKHZ010000000
VERSION     DAVKHZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.169"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            1..703
                     /locus_tag="VWW99_002138"
                     /pseudo
     CDS             1..703
                     /locus_tag="VWW99_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          241 a          132 c          127 g          208 t
ORIGIN      
        1 ccaagtaaaa ggaacaaaac caagaaaagt tgatagagat agacttaaaa actatcttac
       61 tgacaatcca gatgcttatt tgactgaaat agcttctgac tttggctgtc atccaactac
      121 catccactat gcgctcaaag ctatgggcta cactcgaaaa aaagaaccac acctactatg
      181 aacaagaccc agaaaaagta gccttatttc ttaagaattt taatagatta aagcacctag
      241 cacctgttta gattgacgaa acaggattcg atacttattt ttatcgagaa tatggtcgct
      301 cattaaaagg tcagttaata agaggcaaag tatctggaag aagatatcag agaatttctt
      361 tggttgcagg tctaacaaat gaatggtgag ttaatcgctc caatgactta cgaagagacg
      421 atgacgagcg acttttttga agcttggttt cagaagtttc tcttaccaac attaaccaca
      481 ccatcggtta ttattatgga taatgcaaga ttccatagaa tggggaagct agaactcttg
      541 tgtgaagagt ttgggtataa acttttacat cttccttcct actcacctga gtacaatcct
      601 attgagaaaa catgggctca tatcaaaaag cacctcataa aaggtattac caagttgcaa
      661 taccttttat gaggctcttt tgtcttgttc ttgtttcaat tgactata
//
LOCUS       DAVKHZ010000170          702 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000170 DAVKHZ010000000
VERSION     DAVKHZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.170"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..>702
                     /locus_tag="VWW99_002139"
     CDS             <1..>702
                     /locus_tag="VWW99_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9539887.1"
                     /translation="PTDYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFSN
                     KLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTA
                     NIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRI
                     GNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQILERKP
                     KIVGIYKLGMKYNSDN"
BASE COUNT          259 a           86 c          128 g          229 t
ORIGIN      
        1 ccgacagatt atgatgagaa aattggtagt tttaatacat atgaaatcga acaaacggta
       61 tcgaagattc tgagggtaaa acctaatgga aagattattt taaagtcaac agttccgatc
      121 ggcttttcaa acaaattaaa aaggctgttt gatacaaaaa atatcatttt tgtccctgaa
      181 tttttgagag aaggttgttc tatatatgat aatttatatc caagtcgcat agttgttgga
      241 gatgagacag ttgaaggaag aaaaattgca gagttgttcc tttcgattag tactcatagt
      301 actgccaata ttaaaaatgt tatgttagtt tctcctactg aagcagaagc aattaagctt
      361 ttttctaaca cattcttagc tctccgtgtt gcttttttta atgaactaga ttcttttgct
      421 gagaggagaa gtttaaatgc tgaagttgta ataaaaggtg tttgtttaga tccaagaatt
      481 ggaaattttt ataataatcc ttcttttgga tttggaggat attgtcttcc caaagatact
      541 aaacaattaa aaaaagaatt tatagaaata aatgccccag tgatagaagc gattgatatt
      601 tcaaacacaa atagaaaaca gttcatagtt aaacaaatat tggaacgaaa gccaaagata
      661 gtaggaatat ataaattggg gatgaaatat aattcagata at
//
LOCUS       DAVKHZ010000171          651 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000171 DAVKHZ010000000
VERSION     DAVKHZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.171"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..638
                     /locus_tag="VWW99_002140"
     CDS             <1..638
                     /locus_tag="VWW99_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9539888.1"
                     /translation="LSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYG
                     IIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDE
                     VFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRFAIASVMCKTSPFV
                     FFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          254 a           72 c          122 g          203 t
ORIGIN      
        1 aattaagtaa attaaaagtt ccttatttaa aagaaggtgg agagtatcag ataaaaaatc
       61 ttagctacaa atttactgat gatgagtgtt taagcttaaa agatatttca ttcaagcttg
      121 ggaaaattta tggcataata ggatccaacg gacgaggaaa atcaacgctt ttaagatgtt
      181 taataggtct tgagaaaaaa tcaaaagaag aaatttattt taagggagag aagctatcta
      241 aaaaagaaag actcaaaaac tcttcacttg ttatgcaaga tgtaaatcat caattattca
      301 cagatgaagt attcaacgag cttagattag gagtaaagaa ttttgatgaa gaaaaggcga
      361 aaatcatttt aaaagattta ggcctggacg aatttattga aaggcatccg atgagtttat
      421 caggagggca aaagcaaagg tttgcaatag catctgttat gtgcaagact tctccatttg
      481 tcttttttga cgaaccttca agtggtatgg attattccaa tatgataaaa atatctgaac
      541 tgattaataa gtataaaacc atggataaaa taatttttat tgtttcccat gatatagaat
      601 ttttaaatga agtggcagat gaaatttttg aattgtaaaa tagagtctgt t
//
LOCUS       DAVKHZ010000172          624 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000172 DAVKHZ010000000
VERSION     DAVKHZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.172"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            90..386
                     /locus_tag="VWW99_002141"
     CDS             90..386
                     /locus_tag="VWW99_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9539889.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          212 a           79 c          111 g          222 t
ORIGIN      
        1 agacgaacat ttgaagagag ttaggaatac aacatgtgcg aattaactct caataaaaca
       61 tacataatat acaaaaggag tacactaaaa tgaaatctaa aagaatggaa tttcataata
      121 aatttcttat tgtttcagca atgttagcag tcattagttg gttaagctta ggtgttgttt
      181 cttttcctat gttggctggg acattaggaa tttcgacaaa agctgctgct actgtagtca
      241 atttgattag tgcttatagt acagttacag cagtaatttc aattgtaggt gcaatcacag
      301 gagtaggttc tattggttcg ggtattgcag caacagttct ttatatattg aaaaagaaag
      361 gtgctgctaa agcagctcta tggtagaact agatgaaaat actttctaaa aaagaatgtg
      421 aaagtatttt aggttatctt aattttattg ggataaccct tttgtttttt gttatcataa
      481 gtttgtcaat ctattttaaa agctatcttc cttttcaagg gaaattattt ttactttttt
      541 tggtagtaga ttacgctact ttgaatcgtt ataatatgaa actgcctatc caaagtaaaa
      601 aaataaatac tttctatata agga
//
LOCUS       DAVKHZ010000173          600 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000173 DAVKHZ010000000
VERSION     DAVKHZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.173"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..576)
                     /locus_tag="VWW99_002142"
     CDS             complement(<1..576)
                     /locus_tag="VWW99_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
                     /protein_id="HEU9539890.1"
                     /translation="MDAEAEALVDADSDAEVLAEADALVDAEADALVDAEAEALVLAE
                     TDALVDAEAEALVLADSDALVLAEAEALVDAEADALVLAEAEALVDADSDAEVLAEAE
                     TLVDAEADALVLAEAEALVDAEAEADVDADSDALVLAEAEALVDADSEALVDAETEAL
                     VLAEAEALVLAEAEALVDAEAEALVDAETDAL"
BASE COUNT          151 a          194 c          132 g          123 t
ORIGIN      
        1 gagtgcgtca gtctcagcat caacaagtgc ttcagcctca gcatcgacaa gcgcttcagc
       61 ttcagcaagc accagtgctt cggcttcagc aagcacaagt gcttcagtct cagcgtcaac
      121 cagtgcctct gaatccgcat caaccagtgc ctcggcctca gcaagcacaa gtgcgtctga
      181 atccgcatca acgtctgcct cagcctcagc atcgacaagc gcttcagctt cagcaagtac
      241 tagtgcatca gcttccgcat caacaagtgt ctcagcctca gcaagcacct cagcatctga
      301 atcagcgtca accagtgctt cagcctcagc aagtaccagt gcgtcagctt ccgcatcaac
      361 gagtgcttcg gcctcagcaa gtacaagtgc atcagagtca gcaagtacca gtgcctcggc
      421 ttcagcgtca accagtgcat cagtctcagc aagcacaagt gcttcggctt cagcgtcgac
      481 aagtgcgtca gcttcagcat caacgagtgc gtcagcctca gcaagcacct cagcgtctga
      541 atcggcatca acaagtgctt cggcttcagc atcaataagt gcttcagtct cagcatcaac
//
LOCUS       DAVKHZ010000174          594 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000174 DAVKHZ010000000
VERSION     DAVKHZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.174"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            138..389
                     /locus_tag="VWW99_002143"
     CDS             138..389
                     /locus_tag="VWW99_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539891.1"
                     /translation="MSSQMKAFLNELQGNMEVVGEFFDNPEKVIRKFGIVGREKEALL
                     IRDLNDMDDYALSIQNSVASPSGAHSSTCHFVRQDLQSA"
BASE COUNT          215 a           61 c          113 g          205 t
ORIGIN      
        1 attaagcttt aaaaaattat ttttagcaat ttgattttga aagtgtcatg cagcaatatg
       61 aaagcgtaga atagaggtgt ttccaatgta caagtaagtt ttgattccaa ataaagtaat
      121 agaaaaggag ctatataatg agtagtcaaa tgaaagcttt cttgaatgaa cttcaaggaa
      181 atatggaagt tgttggagaa ttttttgata atcctgaaaa ggtaattaga aaatttggaa
      241 ttgtcggaag agagaaggaa gcattgctaa tccgtgactt aaatgatatg gatgattatg
      301 ctctttcaat tcaaaatagc gttgcatcgc cttctggggc acatagtagc acttgccatt
      361 ttgttagaca agatttacaa tccgcataat tgttaatata taaaacataa aatttcaatt
      421 ttcaattagg gataatagaa ttccttacta tgatatagaa agtgaattaa ttaggaaatc
      481 ttagtcagag gtttctgatt aattcatttt ttatatatta agagtgatgt gaaatgttca
      541 aattgtgaga ctactctcgg attgttgtat agaaatttaa aattttattg tgag
//
LOCUS       DAVKHZ010000175          543 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000175 DAVKHZ010000000
VERSION     DAVKHZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.175"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..238
                     /locus_tag="VWW99_002144"
     CDS             <1..238
                     /locus_tag="VWW99_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9539892.1"
                     /translation="IPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFGENKYRL
                     LNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            313..510
                     /locus_tag="VWW99_002145"
     CDS             313..510
                     /locus_tag="VWW99_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9539893.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          189 a           99 c           72 g          183 t
ORIGIN      
        1 aatcccagtt attcctaatt ttaacaatag tttagaggat gcagaaaaat tcgctactct
       61 atttaactca ttaaatatcg accaagttca actactccct tttcatcaat ttggtgaaaa
      121 caaatatcgt ttattaaatc ggaaatatga aatggatgga gtcaacgcac ttcatcctga
      181 agatcttatt gattatcaaa aggtatttct gaaccaccat attaattgtt atttctagtt
      241 tatttccttg aaatgctcta gctatttgca gataacaagc atctatataa ggttatcaaa
      301 gaagacttcc ccatgactct aaatgaatat atcttacaat atcgcctgaa acaagctata
      361 gataagatgg ctgaatctcc caactcctct ttaagcgata tctctgacca agttggattt
      421 tcagactata aatattttgc caaagtattt aaaaagtatc tccatatttc cccaaagaaa
      481 ttgaaatcac tcggaagaat agtaaaatag gctattcttc tttttgtaga gaattttact
      541 cta
//
LOCUS       DAVKHZ010000176          486 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000176 DAVKHZ010000000
VERSION     DAVKHZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.176"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            260..472
                     /locus_tag="VWW99_002146"
     CDS             260..472
                     /locus_tag="VWW99_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9539894.1"
                     /translation="MDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELI
                     KMRFNSSAIDPIYGKKDDASIVVIDV"
BASE COUNT          188 a           48 c           86 g          164 t
ORIGIN      
        1 actatattat cgcttaccaa catttgcttt tatatttgct aaactttcag aaaaaaagtt
       61 ggagtatatg atgctaggtg actgcgtcat gttagtaaac gaaatggaaa taacggatca
      121 tagagtagat aacttatttg aaaaagggaa aaatgagata aaagatccaa ttggtacaaa
      181 tagtgtttta aataaaaaaa ttattttaca aaaaatcaga aaattatcta atcaaccgag
      241 tgggtactgg ataggttcgt tggatgagag atttttagac catgctatta ttaatcaaat
      301 agatgttact agtgaacaaa ttgttttaat gagcgatggc ttttatgaat tctatcaaaa
      361 taatcaaaat aaaacttttg aagagttaat taagatgaga tttaattctt ctgctattga
      421 tccaatctat ggaaagaaag atgatgcaag tattgtagtt attgatgttt gatatattgt
      481 ttaaaa
//
LOCUS       DAVKHZ010000177          472 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000177 DAVKHZ010000000
VERSION     DAVKHZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.177"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            16..234
                     /locus_tag="VWW99_002147"
     CDS             16..234
                     /locus_tag="VWW99_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9539895.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
BASE COUNT          200 a           53 c           90 g          129 t
ORIGIN      
        1 aaaaagaggg atattatggc acttaactat aaaccattat ggatacagtt agcaaaaaaa
       61 ggactaaaga aaacagatgt aatagctatg gcaggactta caacaaatgt tatggcacaa
      121 atgggaaagg ataaaccaat tacatttaag aatttagaaa gaatatgtaa ggctttatct
      181 tgcactccta atgatattat tagttttgaa gataatttta gtgacgagga atagaaaatg
      241 actttaagga cagaagatca agttagggat tatgcaagag aagtataggc tttaatgaag
      301 ttgaagaaaa catcaatcaa ggtactggtc aaataactac ttttaatcaa ttaggcttca
      361 agggatattc agataagcca gatggttggt atttacctaa aaatatgaat gatgtagcaa
      421 taatccttga aacaaaatca gaagaaagag atattagcaa acaaattttt at
//
LOCUS       DAVKHZ010000178          432 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000178 DAVKHZ010000000
VERSION     DAVKHZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.178"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(155..>432)
                     /locus_tag="VWW99_002148"
     CDS             complement(155..>432)
                     /locus_tag="VWW99_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539896.1"
                     /translation="PEKFFGLIEDNLKQVHPLFQTVFKAFLKDKEKIVNALQLHYSNA
                     KLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT          130 a           62 c           88 g          152 t
ORIGIN      
        1 attcaaagag aagtttttga aatttttcta ttactctaga aatagatact ggaagattgt
       61 ggtcatgtat ttcttatcta ttttgtaacc gcccaataac gaagtatatt gaaaaatctc
      121 ggctctttgt caactgtagt gggttgaaaa aaagctaagc tcgagaaagg acaaattttg
      181 tcctttcttt tttgatattc agagcgataa aaatccgttt tttgaagttt tcaaagtttc
      241 gaaaaccaaa ggcattgcgt ttgataagtt tgatgagatt attggtcgct tccaatttgg
      301 cgttagaata gtgtagttga agggcgttga cgattttctc tttgtccttt agaaaggctt
      361 taaagacagt ctgaaaaaga ggatgaacct gctttagatt gtcctcaatg agtccgaaaa
      421 atttctccgg tt
//
LOCUS       DAVKHZ010000179          427 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000179 DAVKHZ010000000
VERSION     DAVKHZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.179"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            183..359
                     /locus_tag="VWW99_002149"
                     /pseudo
     CDS             183..359
                     /locus_tag="VWW99_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          178 a           72 c           64 g          113 t
ORIGIN      
        1 cataaacaca gtatatcatt tttaaatttt aagtcaagga aaattaaccc actttaattt
       61 cttttttaac acgtgttaaa aaagaaatta actgagtaaa aactctcgaa attcaattcg
      121 ctatattatc actcaacttg aacaaatacc agaaccacaa aaaacagatg taaaggaaaa
      181 atatggactt aaaatttgaa ggggttgatt tagaatacaa aaaagctaaa aacaatcttc
      241 cagagtcttt ctgggaaacc tactcagcat ttgcaaatac aaatggcggg aaaataattc
      301 tcgggataga tgaaaaaaac attgacacct accaaagagt caatagactc cctgcgaaac
      361 aaaattatga agcaagtaaa caactaactg atgcacgatt taagagtctt gttggtgttc
      421 agcgtac
//
LOCUS       DAVKHZ010000180          391 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000180 DAVKHZ010000000
VERSION     DAVKHZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.180"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            1..391
                     /locus_tag="VWW99_002150"
                     /pseudo
     CDS             1..391
                     /locus_tag="VWW99_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a           68 c           76 g          106 t
ORIGIN      
        1 attctggtaa aaagaaattt cacgctatga aggctcaagc gattgtcaca agtcaaggga
       61 gaattgtttc tttggatatc actgtgaact attgtcatga tatgaagttg ttcaaaatga
      121 gtcgcagaaa tatcagacaa gctggtaaaa tcttggctga cagtggttat caagggctca
      181 tgaagatata tcctcaagca caaacttcac gtaaatccag caaactcaaa ccgctaacaa
      241 ttgaagataa agtctataac catgcgctat ctaaggagag aagcaaggtt gagaacatct
      301 ttgccaaagt aaaaacgttt aaaatgattt caacaaccta tcgaaatcat ctaaacgctt
      361 cggattacga atgaatttga ttgctggtat t
//
LOCUS       DAVKHZ010000181          377 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000181 DAVKHZ010000000
VERSION     DAVKHZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.181"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(83..377)
                     /gene="tnpB"
                     /locus_tag="VWW99_002151"
                     /pseudo
     CDS             complement(83..377)
                     /gene="tnpB"
                     /locus_tag="VWW99_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          122 a           72 c           64 g          119 t
ORIGIN      
        1 tttctataaa ttaatttgac ttttctgata gagatgttca tatcttattt caattcacta
       61 tactaaaaag aaagtcctca tttcaataga aatcacgact ttctgataaa tttattttgg
      121 gagtgataga aaagcccttc ataagccagt ctatttgttc aggtgtgaga gctttgacat
      181 ccttttatgt actggaccaa gtcagtctgc cgttctcaaa gcgtttatat agtagccaaa
      241 atccttgacc atcccagtaa agggctttaa agcggtcttt acgtccacca caaaagagaa
      301 aaacttgacc ggagaaagga tctaattcaa aatgggtttt aaccagataa gccagtgaat
      361 caatgccttg cctcata
//
LOCUS       DAVKHZ010000182          348 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000182 DAVKHZ010000000
VERSION     DAVKHZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.182"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(<1..>348)
                     /locus_tag="VWW99_002152"
     CDS             complement(<1..>348)
                     /locus_tag="VWW99_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9539897.1"
                     /translation="IMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLR
                     YDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQ
                     FERKSHEYKAIKR"
BASE COUNT          116 a           67 c           66 g           99 t
ORIGIN      
        1 taacgcttga tagccttgta ttcatgagat tttcgttcaa actgattcat aatttgaaca
       61 cgaaaacgac tcatggcacg gctgagatgt tggataatat ggaaacgatc tagaacgatt
      121 ttagcacacg gaaaaagctg tttagccaag tcatagtaag gactaaacat atccatcgta
      181 atgattttca cttgacaacg aacggctcta tcgtagcgaa gaaagtgatt tcggatgaca
      241 gcttgtgttc tgccttcaag aacagtgata atattaagat tatcaaaatc ttgcgcaatg
      301 aaactcatct ttcccttagt gaaggcatac tcatcccaag acataatc
//
LOCUS       DAVKHZ010000183          339 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000183 DAVKHZ010000000
VERSION     DAVKHZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.183"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            231..>339
                     /locus_tag="VWW99_002153"
     CDS             231..>339
                     /locus_tag="VWW99_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9539898.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGL"
BASE COUNT          115 a           53 c           76 g           95 t
ORIGIN      
        1 taccttagtc aagaattaga tgtttcacta tgtttgagta agttgatgat ttcatttgat
       61 aacaggtttg aaactgttag gctaggtggc caaggctaat catagccttg gtttagctga
      121 aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca
      181 cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag atgcaagaac
      241 attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt gagcggtgga
      301 agaatgaaaa taagtccaat cgtgaaattg caggcttgt
//
LOCUS       DAVKHZ010000184          338 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000184 DAVKHZ010000000
VERSION     DAVKHZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.184"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..>338
                     /locus_tag="VWW99_002154"
     CDS             <1..>338
                     /locus_tag="VWW99_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9539899.1"
                     /translation="LKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQV
                     AKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKE
                     KGLTPPSTDH"
BASE COUNT          106 a           72 c           76 g           84 t
ORIGIN      
        1 actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc
       61 tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga
      121 tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga
      181 tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag
      241 ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc
      301 taaagagaaa ggtttgaccc ctccttcgac agaccatc
//
LOCUS       DAVKHZ010000185          312 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000185 DAVKHZ010000000
VERSION     DAVKHZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.185"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
BASE COUNT          115 a           53 c           43 g          101 t
ORIGIN      
        1 aaaattacgt taactttttt cacgaaaagg agaaaattat gcgttacgat tatggatagc
       61 gctttcaaat ttttaaactc ctatcccatc cttttatcta tattatgagc gaaaatataa
      121 taactgtcaa gtaactaaag tgaattttat aaaaaaatta caagccaaat ttgtaaagtt
      181 tacactaagc cgctagggca atcgtctatc agaatgtccg tttatttgtc aataatccga
      241 gaaaatcttg caacgcttag aagtctataa aaactatcaa catttatatg acttgcgaat
      301 agcaatcctg ct
//
LOCUS       DAVKHZ010000186          303 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000186 DAVKHZ010000000
VERSION     DAVKHZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.186"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
BASE COUNT           99 a           58 c           48 g           98 t
ORIGIN      
        1 atttgtcctg ttctcatttc attttactat attttagaag atatgctgtg ctattccaga
       61 ttcagttcgc tatacatgcc cgtcgtacca caaaatcccg tgtttgatga ggcacgggat
      121 ttttggctct ataatatttg tagtgggtaa cccccctatg gatattatgg agcctatttt
      181 gtgtagaaaa aaagtcccat atgacctata atgaaaagcg acaaaacaac tcattagaaa
      241 gaatcatatg gaacaattac attttatcac aaaattacta gacattaaag accctaatat
      301 cca
//
LOCUS       DAVKHZ010000187          300 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000187 DAVKHZ010000000
VERSION     DAVKHZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.187"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..234
                     /locus_tag="VWW99_002155"
     CDS             <1..234
                     /locus_tag="VWW99_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9539900.1"
                     /translation="YNQDKNMCKQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKL
                     IQKGFSLLELTEETSMLSHLKKEVETYYQPKER"
BASE COUNT          103 a           60 c           54 g           83 t
ORIGIN      
        1 tacaatcaag ataaaaacat gtgtaagcaa atctgctaca ctttactaga ggacgccaag
       61 aataagaaaa gctacgatag gctggctatc tgctatgtcc gtattgggat ttgtacagac
      121 gattctaaac ttatccaaaa agggttctcc cttctggagc taaccgagga aacttctatg
      181 ctgtctcatc tcaaaaaaga agtagaaacc tattatcaac cgaaggaaag ataaaaaagc
      241 cgagggattt cctcgactct ttacatatta tttgttcgtt agttcttaat accagccatt
//
LOCUS       DAVKHZ010000188          262 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000188 DAVKHZ010000000
VERSION     DAVKHZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.188"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            complement(80..153)
                     /locus_tag="VWW99_002156"
     tRNA            complement(80..153)
                     /locus_tag="VWW99_002156"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Ile,seq:gat)
     gene            complement(188..258)
                     /locus_tag="VWW99_002157"
     tRNA            complement(188..258)
                     /locus_tag="VWW99_002157"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Gly,seq:tcc)
BASE COUNT           84 a           70 c           55 g           53 t
ORIGIN      
        1 atagtttata cgggaacaag cccgtgtcct cttagtgaac agataatatt ccaatcccct
       61 agtaatggac taaacactat gggcacgagt ggactcgaac caccgacctc acgcttatca
      121 ggcgtgcgct ctaaccacct gagctacgcg cccaagtcaa aaacttggta caaagaacaa
      181 agttcaaagc gggtgacgag aatcgaactc gcgacaacag cttggaaggc tgtagtttta
      241 ccactaaact acacccgcta aa
//
LOCUS       DAVKHZ010000189          251 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000189 DAVKHZ010000000
VERSION     DAVKHZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.189"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
BASE COUNT           84 a           47 c           44 g           76 t
ORIGIN      
        1 aaaaaaactt acttcaagct tgccaatacg aattttaagc agaatatcat ttctcttttt
       61 agaatcaaaa cgacgagatt gtggaatctc tacaggaaca ataggttgtg gcattaaaat
      121 atcctccaat agttttactt tactaatagt atacaggagg agctccatga atgatagata
      181 ccttgttatg acgcgcttac taaacaacta actgatgcac gatttaagcg ccttgttggt
      241 gttcagcgca c
//
LOCUS       DAVKHZ010000190          248 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073742
            SAMN33606149-rid17954573.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVKHZ010000190 DAVKHZ010000000
VERSION     DAVKHZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606149
            Sequence Read Archive: SRR23727679
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722721.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722721.1
            Genome Coverage       :: 256x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,158
            CDSs (total)                      :: 2,118
            Genes (coding)                    :: 1,983
            CDSs (with protein)               :: 1,983
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 67 of 135
            Pseudo Genes (incomplete)         :: 60 of 135
            Pseudo Genes (internal stop)      :: 39 of 135
            Pseudo Genes (multiple problems)  :: 26 of 135
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606149-rid17954573.denovo.190"
                     /strain="AUSMDU00073742"
                     /serotype="21"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST193"
     gene            <1..>248
                     /locus_tag="VWW99_002158"
     CDS             <1..>248
                     /locus_tag="VWW99_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9539901.1"
                     /translation="NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEW
                     FDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT           76 a           51 c           51 g           70 t
ORIGIN      
        1 caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt atgccttaca atcttcaata
       61 tactgtagaa gtcaaaaacg gtagtttaat catacctcat tatgaccatt accataacat
      121 caaatttgag tggtttgacg aaggccttta tgaggcacct aaggggtata ctcttgagga
      181 tcttttggcg actgtcaagt actatgtcga acatccaaac gaacgtccgc attcagataa
      241 tggttttg
//
